The L-type calcium channel alpha 1C subunit gene undergoes extensive, uncoordinated alternative splicing.
The alpha1C subunit is the pore-forming protein for the L-type calcium channel. Previous studies indicate that there is possible tissue-specific alternative splicing of this gene. In this study we cloned the entire open reading frame of the alpha1C subunit cDNA from adult rat cardiac myocytes in a single piece (6.64 kb). Using 75 positive clones that were identified by restriction enzyme mapping, we tested the alternative splicing patterns of the Ca(v) 1.2 gene that encodes the alpha1C subunit protein and focused on five loci: IS6, post-IS6, IIIS2, IVS3, and the c-terminus. The results indicate that: (1) alternative splicing occurs in most of the loci, giving rise to two or three different isoforms at those sites; (2) there is a predominant form for each splicing site, (3) there does not appear to be consistent coordination of splicing at multiple loci of this gene. Alternative splicing is not tissue-specific in most regions.
Fan
Q Ivy
QI
Molecular and Cellular Cardiology, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan, USA.
Vanderpool
Kathleen M
KM
Chung
Hui-San
HS
Marsh
James D
JD
D017398:Alternative Splicing; D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics*; D003001:Cloning, Molecular; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary; D022821:RNA Splice Sites; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015183:Restriction Mapping; D014018:Tissue Distribution
D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C); D020033:Protein Isoforms; D022821:RNA Splice Sites; D012333:RNA, Messenger