title: Mucosal targeting of allergen-loaded microspheres by Aleuria aurantia lectin.
abstract: Murine intestinal M-cells express alpha-L-fucose residues. We constructed alpha-L-fucose-targeting particles for oral immunotherapy of IgE-mediated allergy. Poly(D,L-lactic-co-glycolic acid)-microspheres were loaded with birch pollen allergens, and functionalised with the alpha-L-fucose specific Aleuria aurantia lectin (AAL). The AAL-microspheres had a size of 1-3 microm, protected the entrapped allergens from gastric degradation and released 46.6+/-1.3% allergen over 21 days in vitro. Oral gavages of AAL-particles to naive BALB/c mice induced birch pollen-specific IgG2a, but not IgG1 antibodies. We conclude that targeting allergens to alpha-L-fucose-receptor bearing cells using AAL-microspheres induces specific Th1-antibody responses possibly counteracting Th2-dominated allergy, and therefore provides a potentially useful formulation for oral immunotherapy.
authors.affiliation: Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
authors.forename: Franziska
authors.initials: F
authors.lastname: Roth-Walter
authors.forename: Isabella
authors.initials: I
authors.lastname: Schöll
authors.forename: Eva
authors.initials: E
authors.lastname: Untersmayr
authors.forename: Adolf
authors.initials: A
authors.lastname: Ellinger
authors.forename: George
authors.initials: G
authors.lastname: Boltz-Nitulescu
authors.forename: Otto
authors.initials: O
authors.lastname: Scheiner
authors.forename: Franz
authors.initials: F
authors.lastname: Gabor
authors.forename: Erika
authors.initials: E
authors.lastname: Jensen-Jarolim
mesh_terms: D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D029662:Betula / Q000276:immunology; D018938:Caco-2 Cells; D003888:Desensitization, Immunologic; D020895:Enterocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019344:Lactic Acid / Q000008:administration & dosage*; D037102:Lectins / Q000008:administration & dosage / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011058:Pollen / Q000276:immunology*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D037102:Lectins; D011108:Polymers; C064549:lectin, Aleuria aurantia; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid