title: Carbohydrate supplementation and alterations in neutrophils, and plasma cortisol and myoglobin concentration after intense exercise.
abstract: The present study examined the effect of carbohydrate supplementation on changes in neutrophil counts, and the plasma concentrations of cortisol and myoglobin after intense exercise. Eight well-trained male runners ran on a treadmill for 1 h at 85% maximal oxygen uptake on two separate occasions. In a double-blind cross-over design, subjects consumed either 750 ml of a 10% carbohydrate (CHO) drink or a placebo drink on each occasion. The order of the trials was counter-balanced. Blood was drawn immediately before and after exercise, and 1 h after exercise. Immediately after exercise, neutrophil counts (CHO, 49%; placebo, 65%; P<0.05), plasma concentrations of glucose (CHO, 43%; P<0.05), lactate (CHO, 130%; placebo, 130%; P<0.01), cortisol (CHO, 100%; placebo, 161%; P<0.01), myoglobin (CHO, 194%; placebo, 342%; P<0.01) all increased significantly. One hour post-exercise, plasma myoglobin concentration (CHO, 331%; placebo, 482%; P<0.01) and neutrophil count (CHO, 151%; placebo, 230% P<0.01) both increased further above baseline. CHO significantly attenuated plasma myoglobin concentration and the neutrophil count after exercise (P<0.01), but did not affect plasma cortisol concentration. The effects of CHO on plasma myoglobin concentration may be due to alterations in cytokine synthesis, insulin responses or myoglobin clearance rates from the bloodstream during exercise. Plasma cortisol responses to CHO during exercise may depend on the intensity of exercise, or the amount of CHO consumed. Lastly, cortisol appears to play a minor role in the mobilisation of neutrophils after intense exercise.
authors.affiliation: School of Human Movement Studies, The University of Queensland, St. Lucia, 4072 Brisbane, Queensland, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Peake
authors.forename: Gary
authors.initials: G
authors.lastname: Wilson
authors.forename: Laurel
authors.initials: L
authors.lastname: Mackinnon
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Coombes
mesh_terms: D000328:Adult; D004040:Dietary Carbohydrates / Q000378:metabolism*; D019587:Dietary Supplements; D015444:Exercise / Q000502:physiology*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology; D009211:Myoglobin / Q000097:blood*; D009504:Neutrophils; D010101:Oxygen Consumption; D010919:Placebos; D012420:Running / Q000502:physiology
chemical_list: D004040:Dietary Carbohydrates; D009211:Myoglobin; D010919:Placebos; D006854:Hydrocortisone