title: [Rat alveolar type II injured by bleomycin].
abstract: OBJECTIVE
  To explore dysfunction mechanism of rat alveolar type II (AT-II) injured by bleomycin (BLM).
  
  
  METHODS
  SD rats were injected with a single intratracheal dose of bleomycin or control saline. On day 7, 14, and 28 following intratracheal bleomycin or saline instillation, animals were killed under overdose of 1.5% sodium pentobarbital (0.25 ml/100 g, i.p.) and bronchoalveolar lavage fluid (BALF) from the lung was tested for the activity of pulmonary surfactant (PS) by the Whihelmy Film Balance. Several concentrations of bleomycin stimulated the culture of rat AT-II cells, and surfactant protein (SP) A, B, and aquaporin-1 (AQP) mRNA were analyzed by fluorescent quantitative polymerase chain reaction (FQ-PCR).
  
  
  RESULTS
  The activity of PS and hypoxemia significantly decreased on day 7 and improved on day 14 and completely recovered to normal status on day 28. SP-A, B, and AQP-1 mRNA expression in BLM-stimulated group were significantly lower than those in the control group (P<0.001).
  
  
  CONCLUSIONS
  BLM-injured AT-II cells decrease the levels of SP-A, B, and AQP-1 mRNA and cause PS dysfunction, resulting in hypoxemia and pneumonedema.
authors.affiliation: Laboratory of Cell Biochemistry, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Kong
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Jian-zhao
authors.initials: JZ
authors.lastname: Niu
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Jin
authors.forename: Mei-juan
authors.initials: MJ
authors.lastname: Yang
authors.forename: Ling-qiao
authors.initials: LQ
authors.lastname: Wang
authors.forename: Bing-hua
authors.initials: BH
authors.lastname: Tang
authors.forename: Qiu-ju
authors.initials: QJ
authors.lastname: Zhang
authors.forename: Heng-jing
authors.initials: HJ
authors.lastname: Tu
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000096:biosynthesis* / Q000235:genetics; D001761:Bleomycin / Q000008:administration & dosage / Q000633:toxicity*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D000860:Hypoxia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000187:drug effects; D037662:Pulmonary Surfactant-Associated Protein A / Q000096:biosynthesis* / Q000235:genetics; D037701:Pulmonary Surfactant-Associated Protein B / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; D012333:RNA, Messenger; D001761:Bleomycin; D051398:Aquaporin 1