Protein diversity is generated within the motin family of proteins by alternative pre-mRNA splicing.
The motin family of proteins is comprised of three polypeptides, angiomotin, angiomotin-like 1, and angiomotin-like 2. Angiomotin is an angiostatin-binding protein that promotes endothelial cell motility and is involved in angiogenesis. The function of human angiomotin-like-1 and angiomotin-like-2, however, remains unknown. In this report, we investigated the potential for molecular diversity within the motin family of proteins through the identification and characterization of alternatively spliced transcripts in endothelial cells, human tissues and a variety of cell lines. We report that the motins display variability at the mRNA level suggesting an intricate regulatory system at the transcriptional and potentially protein level. Some alternative transcripts are expressed in a tissue-specific manner and others give rise to novel protein isoforms. The alternative splicing that occurs within this protein family may have important implications in the regulation of the expression and function of the motins.
Moreau
Julie
J
Centre de recherche en rhumatologie et immunologie, Centre de recherche du CHUL, Laval University, Bloc T1-492705, Boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2.
Lord
Mathieu
M
Boucher
Maryse
M
Belleau
Pascal
P
Fernandes
Maria J G
MJ
D017398:Alternative Splicing; D000091082:Angiomotins; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D005091:Exons; D005260:Female; D020869:Gene Expression Profiling; D005796:Genes / Q000235:genetics; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D007438:Introns; D020014:K562 Cells; D008565:Membrane Proteins; D008840:Microfilament Proteins; D008856:Microscopy, Fluorescence; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D014162:Transfection; D020298:U937 Cells
C423985:AMOT protein, human; D000091082:Angiomotins; D002352:Carrier Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D020033:Protein Isoforms; D012322:RNA Precursors; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins