Acetylecholinesterase-based biosensor electrodes for organophosphate pesticide detection. II. Immobilization and stabilization of acetylecholinesterase.
The dry and wet stability of Drosophila acetylcholinesterase non-covalently immobilized onto polyethyleneimine modified screen-printed carbon electrodes was improved when compared to non-immobilized acetylcholinesterase, and acetylcholinesterase covalently immobilized onto dialdehyde and polyethyleneimine modified electrodes. Stabilizer mixtures were characterized for additional stabilization of acetylcholinesterase during storage in the dry state, with dextran-sulphate/sucrose and polygalacturonic acid/sucrose mixtures proving highly effective for long-term storage of biosensor electrodes.
Vakurov
A
A
School of Biochemistry and Microbiology, University of Leeds, Leeds, LS2 9JT, UK.
Simpson
C E
CE
Daly
C L
CL
Gibson
T D
TD
Millner
P A
PA
D000110:Acetylcholinesterase / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D010755:Organophosphates / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D010755:Organophosphates; D010575:Pesticides; D000110:Acetylcholinesterase