[MGB probes detect Streptococcus mutans and Streptococcus sobrinus in real time].
OBJECTIVE
To detect and distinguish Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus) quickly in epidemiology and investigate the distribution of S. mutans in the oral of children with rampant caries.


METHODS
Designed minor groove binder (MGB) probes according to the gtf gene of S. mutans and S. sobrinus. Detected 9 reference strains of Streptococcus mutans group by MGB probes in real time and after cultivation. Evaluated the results of these two methods. 92 dental plaques from pre-school children with rampant caries were detected in real time with MGB probes.


RESULTS
The primers could amplify the target sequences specificity and distinguished S. mutans and S. sobrinus from each other using MGB probes. Though the fluorescence occurred earlier in S. mutans than in S. sobrinus, they had the same results in nature. In 92 children with rampant caries, the detective ratio of S. mutans was 96.7% and that of S. sobrinus was 32.6%. All the samples which could detect S. sobrinus were positive for S. mutans.


CONCLUSIONS
The primers and probe designed from gtf genes of S. mutans and S. sobrinus can amplify the target sequence and distinguish them from each other in real time.
Ling
Jun-qi
JQ
Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Lin
Jia-cheng
JC
Tang
Zhi-ying
ZY
Chen
Han
H
Gao
Yan
Y
D002648:Child; D003731:Dental Caries; D003773:Dental Plaque; D006801:Humans; D013295:Streptococcus mutans; D016986:Streptococcus sobrinus