title: Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae beta-glycosidase.
abstract: The gene encoding beta-glycosidase of the hyperthermophilic archaea Sulfolobus shibatae (SSG) was expressed in Escherichia coli. Recombinant SSG (referred to as rSSG hereafter) was efficiently purified, and its transglycosylation activity was tested with lactose as a donor and various sugars as acceptors. When sucrose was used as an acceptor, we found a distinct intermolecular transglycosylation product and confirmed its presence by TLC and high performance anion exchange chromatography (HPAEC). The sucrose transglycosylation product was isolated by paper chromatography, and its chemical structure was determined by 1H and 13C NMR. The sucrose transfer product was determined to be beta-D-galactopyranosyl-(1-->6)-alpha-D-glucopyranosyl-beta-d-fructofuranoside with a galactose molecule linked to sucrose via a beta-(1-->6)-glycosidic bond.
authors.affiliation: Department of Food Science and Biotechnology and Institute of Life Sciences and Resources, KyungHee University, Yongin 449-701, Republic of Korea.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Park
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Jaeho
authors.initials: J
authors.lastname: Cha
authors.forename: Soo-Bok
authors.initials: SB
authors.lastname: Lee
authors.forename: Joong-Hyuck
authors.initials: JH
authors.lastname: Auh
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
mesh_terms: D002855:Chromatography, Thin Layer; D005959:Glucosidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D013395:Sucrose; D005959:Glucosidases; C104851:Sulfolobus solfataricus beta-glycosidase