title: A fluorescence study of sodium hyaluronate/surfactant interactions in aqueous media.
abstract: The interactions between sodium hyaluronate, an anionic polysaccharide, with surfactants (anionic and nonionic) were investigated using pyrene fluorescence measurement methods. The change of micropolarity produced by the interaction was monitored by the measurement of emission intensity ratio between the first and third bands (I1/I3), and the intensity ratio of the excimer and the third vibration monomer band (I(E)/I(M)). Because the hydrophilic heads on the SDS were attracted by the domains formed by the hydroxyl groups of hyaluronate, the I1/I3 ratio was reduced by the addition of hyaluronate at lower than 0.06% of sodium dodecyl sulfate (SDS) concentration. No aggregation was observed between hyaluronate and nonionic surfactants (Tween-80 and Cremophor EL) in the whole concentration range studied. At a higher concentration of surfactant, the I1/I3 ratio of hyaluronate/surfactant was influenced by the addition of saccharide (glucose, lactose, or mannitol). However, the effect of saccharide could be reduced by the addition of salt.
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.
authors.forename: Dian-Shu
authors.initials: DS
authors.lastname: Yin
authors.forename: Wan-Yun
authors.initials: WY
authors.lastname: Yang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Ge
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D002021:Buffers; D006820:Hyaluronic Acid / Q000737:chemistry*; D011136:Polysorbates / Q000494:pharmacology; D011721:Pyrenes / Q000737:chemistry; D012965:Sodium Chloride / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002021:Buffers; D011136:Polysorbates; D011721:Pyrenes; D012996:Solutions; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D006820:Hyaluronic Acid; C030984:pyrene