Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques.
The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-alpha-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples.
Caputo
Emilia
E
Institute of Genetics and Biophysics-I.G.B., A.Buzzati-Traverso, CNR, Via G. Marconi 10, I-80125 Naples, Italy. caputo@igb.cnr.it
Lombardi
Maria Luisa
ML
Luongo
Vincenza
V
Moharram
Ramy
R
Tornatore
Pete
P
Pirozzi
Giuseppe
G
Guardiola
John
J
Martin
Brian M
BM
D000328:Adult; D000368:Aged; D000510:Alpha-Globulins / Q000032:analysis; D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D020869:Gene Expression Profiling; D006801:Humans; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008545:Melanoma / Q000097:blood*; D009363:Neoplasm Proteins / Q000097:blood*; D010455:Peptides / Q000097:blood*; D040901:Proteomics / Q000379:methods*; D012878:Skin Neoplasms / Q000097:blood*
D000510:Alpha-Globulins; D009363:Neoplasm Proteins; D010455:Peptides; C011877:inter-alpha-inhibitor; D005340:Fibrinogen