title: Capillary zone electrophoresis separation of enantiomers of lisuride.
abstract: Lisuride is an ergot alkaloid derivative with dopaminergic activity. It is used for treatment of Parkinsonism and some other diseases associated with high level of prolactine. Lisuride is a chiral compound derived from natural ergot alkaloids. A new capillary zone electrophoresis (CZE) method capable of separating the enantiomers of lisuride was developed. Using the optimized conditions (acidic electrolyte with the addition of gamma-cyclodextrin (gamma-CD)) as low as 0.02% of undesirable L-lisuride can be detected. Selected method characteristics, i.e., linearity (0-20 mg/l), precision (2.0% at 5 mg/I), and accuracy (101 +/- 4% at 5 mg/l) were evaluated. The optimized method was applied for the analysis of real batches of Lisuride hydrogenmaleate and Lisuride base manufactured by IVAX Pharmaceuticals. It was found that they contain less than 0.02% of undesirable L-enantiomer.
authors.affiliation: Institute of Chemical Technology, Department of Food Preservation and Meat Technology, Technickà 3, Prague 6, Czech Republic. kvasnicf@vscht.cz
authors.forename: Frantisek
authors.initials: F
authors.lastname: Kvasnicka
authors.forename: Bohumír
authors.initials: B
authors.lastname: Bíba
authors.forename: Ladislav
authors.initials: L
authors.lastname: Cvak
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents; D008090:Lisuride / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D047408:gamma-Cyclodextrins
chemical_list: D007202:Indicators and Reagents; D047408:gamma-Cyclodextrins; D008090:Lisuride