title: Interaction and electron transfer between the high molecular weight cytochrome and cytochrome c3 from Desulfovibrio vulgaris Hildenborough: kinetic, microcalorimetric, EPR and electrochemical studies.
abstract: The complex formation between the tetraheme cytochrome c3 and hexadecaheme high molecular weight cytochrome c (Hmc), the structure of which has recently been resolved, has been characterized by cross-linking experiments, EPR, electrochemistry and kinetic analysis, and some key parameters of the interaction were determined. The analysis of electron transfer between [Fe] hydrogenase, cytochrome c3 and Hmc demonstrates a redox-shuttling role of cytochrome c3 in the pathway from hydrogenase to Hmc, and shows an effect of redox state on the interaction between the two cytochromes. The role of polyheme cytochromes in electron transfer from periplasmic hydrogenase to membrane redox proteins is assessed. A model with cytochrome c3 as an intermediate between hydrogenase and various polyheme cytochromes is proposed and its physiological consequences are discussed.
authors.affiliation: Bioénergétique et Ingénierie des Protéines, CNRS, IBSM, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Guiral
authors.forename: Gisèle
authors.initials: G
authors.lastname: Leroy
authors.forename: Pierre
authors.initials: P
authors.lastname: Bianco
authors.forename: Philippe
authors.initials: P
authors.lastname: Gallice
authors.forename: Bruno
authors.initials: B
authors.lastname: Guigliarelli
authors.forename: Mireille
authors.initials: M
authors.lastname: Bruschi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Nitschke
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Giudici-Orticoni
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D003574:Cytochrome c Group / Q000502:physiology*; D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D007700:Kinetics; D008565:Membrane Proteins / Q000502:physiology*; D008970:Molecular Weight; D009876:Operon
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008565:Membrane Proteins; C017165:cytochrome c(3)