TNFR2 gene polymorphism in coronary artery disease.
BACKGROUND
Recently atherosclerosis and coronary artery disease (CAD) are considered to be inflammatory diseases. The genetic polymorphism in inflammatory markers has been well studied and found to be associated with development of CAD.


OBJECTIVE
To study the association of biallelic polymorphism at position 196 in exon 6 of tumor necrosis factor 2 (TNFR2) gene and coronary artery disease.


METHODS
The study design was a prospective case control study conducted at a tertiary referral center mainly catering to the north Indian population.


METHODS
One hundred and fifty angiographically proven patients with coronary artery disease and one hundred and fifty age matched controls were genotyped for TNFR2 gene by polymerase chain reaction followed by analysis of restriction fragment length polymorphism.


METHODS
Genotype frequencies were compared in patients and controls by Chi-square test. Binary logistic regression analysis was used to examine the relationship between genotypes and disease, incorporating other variables into the model.


RESULTS
The incidence of CAD in those with MM genotype was 65% and in those with RM genotype was 42%. Genotype frequency shows significant association of MM genotype with development of CAD (P < 0.001; odds ratio-2.585; 95% confidence interval 1.533-4.359). The association of TNFR2 genotype with CAD persisted on logistic regression analysis.


CONCLUSIONS
MM genotype of TNFR2 gene is associated with development of CAD and RM genotype appears to be protective.
Sankar
V H
VH
Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow - 226014, India.
Girisha
K M
KM
Gilmour
A
A
Singh
V P
VP
Sinha
N
N
Tewari
S
S
Ramesh
V
V
Mastana
S
S
Agrawal
Suraksha
S
D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D003327:Coronary Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015994:Incidence; D007194:India / Q000453:epidemiology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*
D018124:Receptors, Tumor Necrosis Factor