title: Techniques: high-throughput measurement of intracellular Ca(2+) -- back to basics.
abstract: High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, including the regulation and role of intracellular Ca(2+) in a plethora of biological systems. Although an awareness of specific assay conditions is crucial for reliable and reproducible measurements of intracellular free Ca(2+) whichever system of study is used, the complex temporal nature of Ca(2+) signals has posed some unique limitations for its measurement in high-throughput mode. Progress in high-throughput design has overcome many of these limitations and will complement other technical approaches to understanding the underlying regulation and role of intracellular Ca(2+).
authors.affiliation: The School of Pharmacy, The University of Queensland, St Lucia Campus, Brisbane, QLD 4072, Australia. G.Monteith@pharmacy.uq.edu.au
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Monteith
authors.forename: Gary St J
authors.initials: GS
authors.lastname: Bird
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D020013:Calcium Signaling; D002478:Cells, Cultured; D019295:Computational Biology / Q000379:methods*; D015195:Drug Design; D005470:Fluorometry; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans
chemical_list: D019204:GTP-Binding Proteins; D002118:Calcium