title: Lectin chromatography of extrarenal renin protein in human plasma and tissues: potential endocrine function via the renin receptor.
abstract: Secretion of prorenin from extrarenal tissues comprises approximately half of the renin protein in plasma; its origin is unknown. The discovery of a prorenin/renin receptor that activates vascular tissue kinases raises interest in this otherwise inactive component. We have studied its glycosylation as this may distinguish it from renal renin. The binding of renin protein (active and prorenin) in human plasma and tissues to concanavalin (Con A) and wheat germ lectins was deployed. Immunoradiometric and enzyme kinetic assays were applied to column fractions. Mannosylated renin protein binds to Con A and has been shown to be taken up by human vascular and hepatic cells on mannose-6-phosphate receptors, possibly as a clearance mechanism. But the other binding sites of prorenin/renin that elicit a cellular phosphorylation response are apparently independent of glycosylation. The tissues examined (kidney, adrenal, ovary) each contain high proportions of Con A binding renin, but the plasma of normal resting males and females contain mainly non-binding renin, the proportion increasing as renal renin secretion decreases. The extreme of this relationship is seen in anephric patients and in some normal women on oral contraceptives with suppressed renal renin, in whom plasma renin is entirely non-binding to Con A. Conversely, when renal renin secretion was stimulated, the increased plasma active renin bound to Con A. However, extrarenal tissues containing exclusively Con A non-binding renin protein, and hence potential sources of anephric plasma renin protein, were not identified, but are unlikely to include adrenal or female reproductive tract. The findings are consistent with the view that normal human plasma contains a considerable amount of amannosylated prorenin of extrarenal origin that escapes hepatic clearance and has a longer half-life than renal renin. This plasma renin form would be expected to be activated in association with the recently described renin/prorenin aspartyl protease receptor and to participate in local pathophysiological processes.
authors.affiliation: Department of Physiology, The University of Melbourne, Melbourne 3010, Australia.
authors.forename: Anthony J M
authors.initials: AJ
authors.lastname: Stubbs
authors.forename: Sandford L
authors.initials: SL
authors.lastname: Skinner
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D002847:Chromatography, Agarose; D003208:Concanavalin A / Q000378:metabolism; D003276:Contraceptives, Oral / Q000494:pharmacology; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D015592:Immunoradiometric Assay; D007668:Kidney / Q000378:metabolism; D008297:Male; D009392:Nephrectomy; D010053:Ovary / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000502:physiology*; D012083:Renin / Q000097:blood* / Q000378:metabolism / Q000502:physiology; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D003276:Contraceptives, Oral; D011956:Receptors, Cell Surface; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D012083:Renin