Formation of loops in DNA under tension.
We study the formation of loops along a DNA molecule under applied tension, as might occur in single-DNA micromanipulation experiments with proteins which are able to simultaneously bind two DNA sites. We consider the case of "bare" DNA in the loop, which forms a "teardrop" shape, and the case where a single DNA-bending protein produces a "kink" in the middle of the loop; the presence of a right-angle kink in the loop reduces its bending energy by a factor of 3. Using the bending energy plus an estimate of the free energies associated with fluctuations and the elasticity of the extended nonlooped DNA, we obtain a probability distribution for loops as a function of loop size and force. Force strongly suppresses formation of all loops, but suppresses large loops more severely than small ones. This quenching effect of force is reduced in the presence of a kink in the loop. We also calculate the speed at which length is absorbed into loops between arbitrary positions along the DNA (i.e., for non-sequence-specific loop forming proteins). The speed of retraction of the molecule decays as a stretched exponential function of the force with characteristic force scales depending on the geometry of the loops.
Sankararaman
Sumithra
S
Department of Physics, University of Illinois at Chicago, 845 W Taylor Street, Chicago, Illinois 60607-7059, USA. sumithra@uic.edu
Marko
John F
JF
D003198:Computer Simulation; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004548:Elasticity; D008846:Micromanipulation / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010812:Physical Stimulation / Q000379:methods*; D013314:Stress, Mechanical; D013718:Tensile Strength
D004247:DNA