title: Overexpression of Bop3 confers resistance to methylmercury in Saccharomyces cerevisiae through interaction with other proteins such as Fkh1, Rts1, and Msn2.
abstract: We found that overexpression of Bop3, a protein of unknown function, confers resistance to methylmercury in Saccharomyces cerevisiae. Bmh2, Fkh1, and Rts1 are proteins that have been previously shown to bind Bop3 by the two-hybrid method. Overexpression of Bmh2 and the homologous protein Bmh1 confers resistance to methylmercury in yeast, but overexpression of either Fkh1 or Rts1 has a minimal effect. However, the increased level of resistance to methylmercury produced by overexpression of Bop3 was smaller in Fhk1-deleted yeast as compared with that of the wild-type strain. In contrast, the degree of resistance was significantly elevated in Rts1-deleted yeast. Msn2 and Msn4 were previously reported as proteins that bind to Bmh1 and Bmh2. Overexpression of Msn2 conferred a much greater sensitivity to methylmercury in yeast, while deletion of the corresponding gene lowered the degree of resistance to methylmercury induced by overexpression of Bop3. These results suggest that multiple proteins are involved in minimizing the toxicity of methylmercury induced by overexpression of Bop3.
authors.affiliation: Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Gi-Wook
authors.initials: GW
authors.lastname: Hwang
authors.forename: Yuko
authors.initials: Y
authors.lastname: Furuoya
authors.forename: Ayano
authors.initials: A
authors.lastname: Hiroshima
authors.forename: Takemitsu
authors.initials: T
authors.lastname: Furuchi
authors.forename: Akira
authors.initials: A
authors.lastname: Naganuma
mesh_terms: D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D051858:Forkhead Transcription Factors; D008767:Methylmercury Compounds / Q000494:pharmacology*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; C464906:Fkh1 protein, S cerevisiae; D051858:Forkhead Transcription Factors; C081935:MSN2 protein, S cerevisiae; D008767:Methylmercury Compounds; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors