title: High-resolution analysis of the subtelomeric regions of human embryonic stem cells.
abstract: The use of human embryonic stem cells (hESCs) in most applications is dependent on their undifferentiated proliferation in vitro. Recent studies have illustrated the possibility that chromosomal changes may occur in hESCs during in vitro propagation of these cells. However, no studies so far have screened for chromosomal abnormalities in hESCs using high-resolution techniques that can detect alterations on a few base-pair levels. We have used the recently developed multiplex ligation-dependent probe amplification procedure to analyze the possible occurrence of deletions or duplications in the subtelomeric regions of hESCs in early and late passages. In this study we show that no subtelomeric anomalies were detected in any of the nine hESC lines investigated, supporting the conclusion that hESCs, under appropriate conditions, maintain genomic stability during in vitro propagation.
authors.affiliation: Department of Clinical Genetics, Sahlgrenska University Hospital East, 416-85 Göteborg, Sweden. catarina.darnfors@vgregion.se
authors.forename: Catarina
authors.initials: C
authors.lastname: Darnfors
authors.forename: Anna
authors.initials: A
authors.lastname: Flodin
authors.forename: Katarina
authors.initials: K
authors.lastname: Andersson
authors.forename: Gunilla
authors.initials: G
authors.lastname: Caisander
authors.forename: Jenny
authors.initials: J
authors.lastname: Lindqvist
authors.forename: Johan
authors.initials: J
authors.lastname: Hyllner
authors.forename: Jan
authors.initials: J
authors.lastname: Wahlström
authors.forename: Peter
authors.initials: P
authors.lastname: Sartipy
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D016615:Telomere / Q000648:ultrastructure*