Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription.
Yeast cells adapted to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) exhibit a plasma membrane less susceptible to 2,4-D-induced disruption and are more tolerant than unadapted cells to lethal concentrations of the herbicide. These cells, adapted to grow in the presence of increasing concentrations of 2,4-D, were found to exhibit a dose-dependent increase of the saturation degree of membrane fatty acids, associated to the higher percentage of stearic (C(18:0)) and palmitic (C(16:0)) acids, and to the decreased percentage of palmitoleic (Delta9-cisC(16:1)) and oleic (Delta9-cisC(18:1)) acids. The decreased transcription of the OLE1 gene (encoding the Delta9 fatty acid desaturase that catalyses the conversion of palmitic and stearic acids to palmitoleic and oleic acids, respectively) registered in 2,4-D adapted cells suggests that yeast adaptation to the herbicide involves the enhancement of the ratio of saturated (C(16:0) and C(18:0)) to monounsaturated (C(16:1) and C(18:1)) membrane fatty acids through a reduced OLE1 expression.
Viegas
Cristina A
CA
Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
Cabral
M Guadalupe
MG
Teixeira
Miguel C
MC
Neumann
Grit
G
Heipieper
Hermann J
HJ
Sá-Correia
Isabel
I
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects*; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D017931:DNA Primers; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase; D014158:Transcription, Genetic / Q000502:physiology*
D017931:DNA Primers; D005227:Fatty Acids; D015084:2,4-Dichlorophenoxyacetic Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase