title: Antibody against the carboxyl terminus of intimin alpha reduces enteropathogenic Escherichia coli adherence to tissue culture cells and subsequent induction of actin polymerization.
abstract: The C-terminal third of intimin binds to its translocated receptor (Tir) to promote attaching and effacing lesion formation during infection with enteropathogenic Escherichia coli (EPEC). We observed that the adherence of EPEC strains to HEp-2 cells was reduced and that actin polymerization was blocked by antibody raised against the C-terminal third of intimin alpha.
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799, USA.
authors.forename: Humberto M
authors.initials: HM
authors.lastname: Carvalho
authors.forename: Louise D
authors.initials: LD
authors.lastname: Teel
authors.forename: John F
authors.initials: JF
authors.lastname: Kokai-Kun
authors.forename: Alison D
authors.initials: AD
authors.lastname: O'Brien
mesh_terms: D000199:Actins / Q000378:metabolism*; D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D001422:Bacterial Adhesion; D001665:Binding Sites; D002478:Cells, Cultured; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000276:immunology*; D005434:Flow Cytometry; D006041:Goats; D006801:Humans; D011108:Polymers / Q000378:metabolism
chemical_list: D000199:Actins; D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; D011108:Polymers; C073819:eaeA protein, E coli