Bone marrow-derived lin(-)c-kit(+)Sca-1+ stem cells do not contribute to vasculogenesis in Lewis lung carcinoma.
The development of tumor vasculature is thought to occur through two complementary processes: sprouting angiogenesis from preexisting blood vessels of the host, and vasculogenesis, which involves the spontaneous development of vessels through specific recruitment, differentiation, and vascular incorporation of circulating endothelial cells (EC), endothelial progenitor cells (EPC), or potentially bone marrow-derived cells. Recent reports, however, have challenged the belief that bone marrow-derived cells contribute to tumor neovascularization, claiming an exclusive role for sprouting angiogenesis in tumor blood vessel development. In the present study, we explored the recruitment behavior of bone marrow-derived lin(-)c-kit(+)Sca-1+ stem cells to subcutaneously implanted Lewis lung carcinoma in a syngeneic bone marrow transplantation model. We observed that although lin(-)c-kit(+)Sca-1+ and their derived cells demonstrate significant recruitment to carcinomas in vivo, they do not appear to functionally contribute to tumor neovascularization. Furthermore, our results support the hypothesis that new vessel formation in carcinomas occurs primarily through endothelialization from adjacent and preexisting vasculature.
Shinde Patil
Vivek R
VR
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
Friedrich
Erik B
EB
Wolley
Allison E
AE
Gerszten
Robert E
RE
Allport
Jennifer R
JR
Weissleder
Ralph
R
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics* / Q000502:physiology*; D001853:Bone Marrow / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D002277:Carcinoma; D018827:Carcinoma, Lewis Lung / Q000473:pathology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D019070:Cell Lineage; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000473:pathology; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D009942:Organometallic Compounds / Q000737:chemistry; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000737:chemistry; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics* / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology; D014018:Tissue Distribution
D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline; D019009:Proto-Oncogene Proteins c-kit