[The construction of recombinant plasmid with prostate-specific membrane antigen promoter controlling reporter gene expression].
OBJECTIVE
To study the specificity of prostate-specific membrane antigen (PSMA) promoter in controlling gene expression.


METHODS
PSMA promoter gene was amplified with PCR, and then the promoter gene was cloned into the vector pEGFP-1 to construct a recombinant plasmid, which was transfected into different cell lines such as LNcap, PC-3, MCF-7, A549. Green fluorescent protein (GFP) expression was observed.


RESULTS
The recombinant plasmid constructed, and PSMA promoter uniquely showed modulating activity in PSMA positive cell line.


CONCLUSIONS
PSMA promoter possesses PSMA positive cell specificity, as well as prostatic tissue specificity. PSMA promoter may have the potential for use in targeted gene therapy of prostate adenocarcinoma.
Zeng
Hao
H
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Hong
H
Liao
Yong-chuan
YC
Li
Xiang
X
Wu
Qi
Q
Yang
Yu-ru
YR
D000230:Adenocarcinoma / Q000628:therapy; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D043425:Glutamate Carboxypeptidase II / Q000096:biosynthesis / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000276:immunology* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000628:therapy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D012333:RNA, Messenger; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II