[A laplace transform method for analysis of fluorescence dynamics].
A Laplace transform formula is developed for analysis of a multi-exponential fluorescence dynamic process. A dynamic spectrum function is introduced, calculation of which directly yields parameters of the corresponding dynamic process. The formula is applied to analyze fluorescence dynamics of beta subunit from R-phycoerythrin. It is demonstrated that electronic excited-states of the biomolecular system exhibit two exponential decay processes, the faster of which relates to excitation equilibrium among chromophores, and the slower to radiative relaxation of the chromophores.
Zheng
X
X
Institute for Laser and Spectroscopy, Zhongshan University, 510275 Guangzhou.
Wang
H
H
Yu
Z
Z
Gao
Z
Z