title: Inhibition of production of reactive oxygen species and gene expression profile by treatment of ethanol extract of Moutan Cortex Radicis in oxidative stressed PC12 cells.
abstract: Moutan Cortex Radicis (MCR) is one of the most widely used Oriental medicines. In this study, we assessed the reducing effect of ethanol extract of MCR on hydrogen peroxide-induced reactive oxygen production, the main cause of cell damage or death in PC12 cells. The viability of cells treated with 1 mg/ml of MCR was significantly restored from that of oxidative-stressed PC12 cells. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H(2)DCFDA assay. MCR at 1-0.01 mg/ml concentration inhibited ROS production in oxidative-stressed cells. To identify candidate genes responsible for the anti-oxidative effects of MCR on PC12 cells, an oligonucleotide microarray analysis was performed. The result of gene expression profiles showed that 10 genes were up-regulated and 7 were down-regulated in MCR plus hydrogen peroxide treated cells compared with hydrogen peroxide treated cells. Among them, heme oxygenase (HO) and cathechol-O-methyltransferase (COMT) are related to regulation of ROS generation and the others are known to regulate cell survival and progression. Subsequently, we performed real-time RT-PCR to quantify the ROS related gene. MCR treatment increased the expression of HO by 370% and COMT by 280% at the concentration of 1 mg/ml. These findings suggest that MCR inhibits the production of ROS and cytotoxicity by oxidative-stressed PC12 cells through over-expression of HO and COMT.
authors.affiliation: College of Oriental Medicine, Kyung-Hee University, Seoul, Republic of Korea.
authors.forename: Samwoong
authors.initials: S
authors.lastname: Rho
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Moonkyu
authors.initials: M
authors.lastname: Kang
authors.forename: Euna
authors.initials: E
authors.lastname: Lee
authors.forename: Chongwoon
authors.initials: C
authors.lastname: Cho
authors.forename: Hyunhee
authors.initials: H
authors.lastname: Kim
authors.forename: Seongkyu
authors.initials: S
authors.lastname: Park
authors.forename: Hong-Yeoul
authors.initials: HY
authors.lastname: Kim
authors.forename: Moonchang
authors.initials: M
authors.lastname: Hong
authors.forename: Minkyu
authors.initials: M
authors.lastname: Shin
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D015536:Down-Regulation; D020869:Gene Expression Profiling; D009490:Neurosecretory Systems / Q000187:drug effects; D018384:Oxidative Stress; D016716:PC12 Cells; D029596:Paeonia; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species