Tethered or adsorbed supported lipid bilayers in nanotubes characterized by deuterium magic angle spinning NMR spectroscopy.
2H solid-state NMR experiments were performed under magic angle spinning on lipid bilayers oriented into nanotubes arrays, as a new method to assess the geometrical arrangement of the lipids. Orientational information is obtained from the intensities of the spinning sidebands. The lipid bilayers are formed by fusion of small unilamellar vesicles of DMPC-d54 inside a nanoporous anodic aluminum oxide, either by direct adsorption on the support or by tethering through a streptavidin/biotin linker. The results support that the quality of the lipid bilayers alignment is clearly in favor of the tethering rather than an adsorbed strategy.
Wattraint
Olivier
O
Warschawski
Dror E
DE
Sarazin
Catherine
C
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D016062:Porosity; D019809:Streptavidin / Q000737:chemistry
D008051:Lipid Bilayers; D001710:Biotin; D019809:Streptavidin; D003903:Deuterium; D000537:Aluminum Oxide; D004134:Dimyristoylphosphatidylcholine