Effect of argon-plasma treatment on proliferation of human-skin-derived fibroblast on chitosan membrane in vitro.
Chitosan is not only a nontoxic, biocompatible, and biodegradable polymer, but has also a chemical structure similar to glycosaminoglycans (GAGs), which promote scarless wound healing of skin. In this study, chitosan membranes were treated with argon plasma to improve their surface hydrophilicity. The results showed that the water contact angles of these surface-treated membranes were significantly reduced from 60.76 to 11.57 degrees . The total surface energy was increased from 41.06 to 67.31 mJ/m(2), with 60-86.95% improvement in the gamma-negative component and a 20% difference in the nonpolar component. Argon-plasma-treated chitosan membranes exhibited excellent attachment, migration, and proliferation of the human-skin-derived fibroblasts (hSFs) compared to the untreated ones. It was found that the duration of argon-plasma treatment influenced the cell proliferation, and the optical densities in MTT assay were enhanced. Argon-plasma treatment improved the surface hydrophilicity of chitosan membranes and promoted the attachment and proliferation of hSFs.
Zhu
Xiao
X
School of Mechanical and Production Engineering, Nanyang Technological University, Singapore.
Chian
Kerm Sin
KS
Chan-Park
Mary Bee Eng
MB
Lee
Seng Teik
ST
D001128:Argon; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008567:Membranes, Artificial; D015394:Molecular Structure; D013499:Surface Properties
D008567:Membranes, Artificial; D001128:Argon; D048271:Chitosan