Spectrophotometric determination of oxiconazole in topical lotion using methyl orange.
A spectrophotometric method is described for the determination of oxiconazole in raw material and in topical lotion. This method is based on the reaction of the oxiconazole with methyl orange in buffered aqueous solution of citric acid at pH 2.3. The chromogen, being extractable with dichloromethane, could be measured quantitatively with maximum absorption at 427 nm. The Lambert-Beer law was obeyed in the concentration range of 4.0-14.0 microg ml(-1). A prospective validation of the method showed that the method was linear (r=0.9995), precise (intra-day: CV=1.57% and inter-day: CV=1.50%) and accurate (mean recoveries: 99.69%). The results compared favourably with those of the HPLC method.
Milano
Julie
J
Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, CEP 97119-900-RS, Brazil.
Cardoso
Simone Gonçalves
SG
D000287:Administration, Topical; D000935:Antifungal Agents / Q000032:analysis*; D001391:Azo Compounds / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D001391:Azo Compounds; D007093:Imidazoles; D007202:Indicators and Reagents; C100258:methyl orange; C022155:oxiconazole