Cholesterol as target of Fe-NTA-induced lipid peroxidation in rat tissues.
Intraperitoneal injection of the iron-chelate, ferric-nitrilotriacetate (Fe-NTA), induces renal proximal tubular damage associated with oxidative damage in vivo. A sub-lethal dose of Fe-NTA (15 mg Fe/kg body weight) was administered IP to rats; animals were sacrificed and liver, kidney and plasma were collected 1-4 h after injection. In response to the Fe-NTA administration, there were significant time-dependent reductions of the levels of total lipids, cholesterol and total unsaturated fatty acids, and a rise in the concentrations of conjugated dienes, 7-ketocholesterol and fatty acids hydroperoxides, showing a pattern inversely correlated in plasma, kidney and liver. Cholesterol level decreased significantly from 1 h after injection in the kidney and 3-4 h in the plasma and liver of treated rats. This is the first report on cholesterol reduction and accumulated 7-ketocholesterol in the tissues of rats treated with Fe-NTA as a consequence of lipid peroxidation.
Rosa
Antonella
A
Dip. Biologia Sperimentale, Sez. Patologia Sperimentale, Università degli Studi di Cagliari, Cittadella Universitaria, Km 4.5 SS 554, 09042 Monserrato, Cagliari, Italy. anrosa@unica.it
Deiana
Monica
M
Corona
Giulia
G
Atzeri
Angela
A
Incani
Alessandra
A
Dessì
M Assunta
MA
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002784:Cholesterol / Q000378:metabolism*; D005290:Ferric Compounds / Q000633:toxicity*; D007263:Infusions, Parenteral; D007668:Kidney / Q000737:chemistry / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D005290:Ferric Compounds; D002784:Cholesterol; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate