Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: confocal surface-enhanced Raman spectroscopic study.
Rapid and highly sensitive detection of duplex dye-labelled DNA sequences in a PDMS microfluidic channel was investigated using confocal surface enhanced Raman spectroscopy (SERS). This method does not need either an immobilization procedure or a PCR amplification procedure, which are essential for a DNA microarray chip. Furthermore, Raman peaks of each dye-labelled DNA can be easily resolved since they are much narrower than the corresponding broad fluorescence bands. To find the potential applicability of confocal SERS for sensitive bio-detection in a microfluidic channel, the mixture of two different dye-labelled (TAMRA and Cy3) sex determining Y genes, SRY and SPGY1, was adsorbed on silver colloids in the alligator teeth-shaped PDMS microfluidic channel and its SERS signals were measured under flowing conditions. Its major SERS peaks were observable down to the concentration of 10(-11) M. In the present study, we explore the feasibility of confocal SERS for the highly sensitive detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip.
Park
Taehan
T
Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
Lee
Sangyeop
S
Seong
Gi Hun
GH
Choo
Jaebum
J
Lee
Eun Kyu
EK
Kim
Yang S
YS
Ji
Won Ho
WH
Hwang
Seung Yong
SY
Gweon
Dae-Gab
DG
Lee
Sanghoon
S
D003102:Colloids / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D013499:Surface Properties
D003102:Colloids; D004396:Coloring Agents; D004129:Dimethylpolysiloxanes; D009841:Oligonucleotides; D012834:Silver; D004247:DNA