In vitro and in vivo assays to assess the functions of calnexin and calreticulin in ER protein folding and quality control.
Newly synthesized polypeptides entering the endoplasmic reticulum (ER) encounter a large array of molecular chaperones and folding factors that facilitate proper folding as well as assess folding status, retaining non-native proteins within the ER. Calnexin (CNX), an ER membrane protein, and its soluble homologue, calreticulin (CRT), are two important molecular chaperones that contribute to both processes. They are highly unusual chaperones in that they act as lectins, binding the Asn-linked oligosaccharides of newly synthesized glycoproteins, as well as recognizing the polypeptide segments of glycoproteins. Furthermore, they associate with ERp57, a thiol oxidoreductase, that is thought to enhance the oxidative folding of glycoproteins bound to CNX/CRT. These characteristics of CNX and CRT as well as their mode of action have been elucidated though the use of multiple in vitro and in vivo approaches. This chapter will focus on the description of a number of in vitro assays that have been used to characterize the lectin and ERp57-binding functions of CNX/CRT and also their abilities to act as molecular chaperones to suppress protein aggregation. In addition, we will describe insect and mammalian expression systems in which major histocompatibility complex class I molecules are used as model glycoprotein substrates for CNX and CRT. These systems have been valuable in assessing folding and quality control events in vivo that are influenced by CNX or CRT as well as in characterizing the spectrum of substrates that are recognized by these chaperones.
Paquet
Marie-Eve
ME
Department of Biochemistry, University of Toronto, Toronto, Canada M5S 1A8.
Leach
Michael R
MR
Williams
David B
DB
D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001681:Biological Assay; D037281:Calnexin / Q000235:genetics / Q000502:physiology*; D037282:Calreticulin / Q000235:genetics / Q000502:physiology*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007535:Isomerases / Q000378:metabolism*; D008958:Models, Molecular; D017510:Protein Folding
D037282:Calreticulin; D006360:Heat-Shock Proteins; D037281:Calnexin; D007535:Isomerases