title: Insulin-like growth factors I and II in the aseptic loosening of total hip implants.
abstract: Periprosthetic interface tissue and pseudocapsule samples surrounding aseptically loosened hip implants and control knee synovium were studied by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Endothelial cells, fibroblasts, and monocyte/macrophages contained bone formation-enhancing insulin-like growth factors (IGFs). In interface tissue we found fewer IGF-I and IGF-II positive cells than in control tissue. In pseudocapsular tissue we found fewer IGF-I positive cells and an equal amount of IGF-II positive cells compared to control tissues. Decreased bone formation may contribute to net loss of bone around aseptically loosened hip implants.
authors.affiliation: Molecular Biology Laboratory, Musculoskeletal Diseases and Inflammation Research Group (TULES RYHMA), Institute of Biomedicine, Department of Anatomy, University of Helsinki, Helsinki, Finland. Ville.Waris@helsinki.fi
authors.forename: V
authors.initials: V
authors.lastname: Waris
authors.forename: D S
authors.initials: DS
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Leminen
authors.forename: S
authors.initials: S
authors.lastname: Santavirta
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: L
authors.initials: L
authors.lastname: Nordsletten
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects*; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D016022:Case-Control Studies; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000378:metabolism*; D007593:Joint Instability / Q000175:diagnosis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011475:Prosthesis Failure; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018570:Risk Assessment; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II