title: T-cell activation is accompanied by an ubiquitination process occurring at the immunological synapse.
abstract: The immunological synapse (IS) is a specialized signaling area formed at the contact site between T-cells and antigen-presenting cells (APC), where sustained engagement and signaling of TCR and accessory molecules occur. A key feature of T-cell antigen recognition is that the process of TCR/peptide-MHC interaction is self-limited by the internalization and degradation of triggered TCR and recruited signaling components. The mechanism of signaling component degradation involves their ubiquitination and targeting for degradation. Yet, the relationship between the ubiquitination process and TCR signaling as well as the cellular localization of TCR-induced ubiquitination are still elusive. In the present work, we visualize for the first time ubiquitination at the TCR signaling area. We show an enrichment of ubiquitin staining in TCR/CD3 caps in T-lymphocytes stimulated by anti-CD3 antibodies. Remarkably, we also show the recruitment of the ubiquitin ligase Cbl-b and a significant ubiquitination at the immunological synapse in antigen-stimulated T-cells. Our results identify the immunological synapse as the cellular area where TCR-induced protein ubiquitination occurs. They imply that the synapse is a specialized site where the activation process is not only triggered, but also controlled via ubiquitination of signaling actors.
authors.affiliation: INSERM U563, Institut Claude de Preval, CHU Purpan, 31059 Toulouse Cedex 3, France.
authors.forename: Aurelie
authors.initials: A
authors.lastname: Wiedemann
authors.forename: Sabina
authors.initials: S
authors.lastname: Müller
authors.forename: Benoit
authors.initials: B
authors.lastname: Favier
authors.forename: Doris
authors.initials: D
authors.lastname: Penna
authors.forename: Martine
authors.initials: M
authors.lastname: Guiraud
authors.forename: Christelle
authors.initials: C
authors.lastname: Delmas
authors.forename: Eric
authors.initials: E
authors.lastname: Champagne
authors.forename: Salvatore
authors.initials: S
authors.lastname: Valitutti
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology / Q000502:physiology*; D050721:Proto-Oncogene Proteins c-cbl; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D011948:Receptors, Antigen, T-Cell; D025801:Ubiquitin; C492211:CBLB protein, human; D050721:Proto-Oncogene Proteins c-cbl; D044767:Ubiquitin-Protein Ligases