title: The association of the carrier state of the tumor necrosis factor-alpha (TNFalpha) -308A allele with the duration of oxygen supplementation in preterm neonates.
abstract: BACKGROUND
  High levels of inflammatory cytokines lead to lung damage in premature newborns. We investigated whether single nucleotide polymorphisms (SNP) of innate immunity cytokine genes influence the length of oxygen supplementation.
  
  
  METHODS
  We genotyped 123 very low birth weight (VLBW) infants for the tumour necrosis factor (TNF)- alpha G(-308)A, interleukin (IL)-1beta C(3954)T, IL-6 G(-174)C and IL-10 G(-1082)A SNPs. Genomic DNA was isolated from remnant dried blood samples from the neonates. We tested the association between SNPs and ventilation characteristics using a stepwise multiple regression analysis model.
  
  
  RESULTS
  The carrier state of the TNF-alpha G(-308)A allele was associated with a 40-hour longer period of mechanical ventilation (p=0.004) and, on average, an additional 36 hours of oxygen supplementation (p=0.0008). The association was significant after its adjustment for perinatal risk factors for lung damage.
  
  
  CONCLUSIONS
  The TNF-alpha G(308)A genotype - which is associated with increased TNF-alpha levels - might influence the supplemental oxygen requirement of VLBW infants.
authors.affiliation: First Department of Pediatrics, Semmelweis University, Bókay u. 53, H-1083 Budapest, Hungary.
authors.forename: Géza
authors.initials: G
authors.lastname: Bokodi
authors.forename: András
authors.initials: A
authors.lastname: Treszl
authors.forename: László
authors.initials: L
authors.lastname: Derzbach
authors.forename: Adám
authors.initials: A
authors.lastname: Balogh
authors.forename: Barna
authors.initials: B
authors.lastname: Vásárhelyi
mesh_terms: D000483:Alleles; D005260:Female; D005838:Genotype; D005865:Gestational Age; D006579:Heterozygote; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007223:Infant; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D020641:Polymorphism, Single Nucleotide; D012044:Regression Analysis; D012121:Respiration, Artificial; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D010100:Oxygen