title: Determination of deracoxib in feline plasma samples using high performance liquid chromatography.
abstract: A new HPLC procedure for the determination of deracoxib, a selective cyclooxygenase-2 inhibitor, has been developed and validated. Following a liquid-liquid extraction using isopropyl alcohol and chloroform, samples were separated by isocratic reversed-phase HPLC on an Atlantis C18 column and quantified using UV detection at 252 nm. The mobile phase was a mixture of 10 mM potassium phosphate (pH 4.5) and acetonitrile, with a flow-rate of 1.0 ml/min. The procedure produced a linear curve over the concentration range 10-1500 ng/ml. The development of the assay allowed the determination of pharmacokinetic parameters after oral administration of deracoxib in cats and would be suitable for other pharmacokinetic studies.
authors.affiliation: Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37966, USA. scox6@utk.edu
authors.forename: Sherry K
authors.initials: SK
authors.lastname: Cox
authors.forename: Jacob
authors.initials: J
authors.lastname: Roark
authors.forename: Adam
authors.initials: A
authors.lastname: Gassel
authors.forename: Karen
authors.initials: K
authors.lastname: Tobias
mesh_terms: D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000097:blood*; D000096926:Benzenesulfonamides
chemical_list: D016861:Cyclooxygenase Inhibitors; D013449:Sulfonamides; C471996:deracoxib; D000096926:Benzenesulfonamides