Surface modification of liposomes for cardiomyocytes targeting in vitro.
The effect of novel 3-{4-[2-hydroxyl-(1-methyl ethylamine) propyl oxygen]phenyl}propionic acid cetylester (PAC) as a surface modification ligand on the delivery of liposomes into cultured cardiomyocytes was investigated. Small unilamellar liposomes with and without PAC (PAC-liposome and Plain-liposome) were labeled with a fluorescence marker. The cultured neonatal cardiomyocytes were incubated with liposomes under normoxia or hypoxia conditions, and then the cell-associated fluorescence was measured. A high affinity of the PAC-liposomes to cardiomyocytes was observed. The amount of cell uptake of PAC-liposomes under normoxia conditions was 4-fold higher than that of plain-liposome, and the increase was 8.5-fold when hypoxia occured. The results suggested that PAC is a potential surface modification ligand for liposome targeting the ischemic myocardium.
Chen
Y
Y
Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, Liaoning, PR China. w.chenyan@263.net
Deng
Y J
YJ
Hao
Y L
YL
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008024:Ligands; D008081:Liposomes; D017202:Myocardial Ischemia / Q000188:drug therapy; D032383:Myocytes, Cardiac / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties
D004337:Drug Carriers; D008024:Ligands; D008081:Liposomes