title: Morphine modulates inducible nitric oxide synthase expression and reduces pulmonary oedema induced by alpha-naphthylthiourea.
abstract: This study was designed to investigate the possible participation of morphine in pulmonary oedema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent in the lung. Injection of ANTU (15 mg/kg i.p.) produced pulmonary oedema as indicated by an increase in lung weight/body weight ratio and pleural effusion reaching a maximum within 4 h in rat. Administration of morphine prior to ANTU significantly inhibited to pulmonary oedema with a dose-dependent manner. The protective effect of morphine is prevented by peripheral opioid receptor antagonist, naloxone methiodide. ANTU-treated rats were shown positive by inducible nitric oxide synthase immunohistochemical staining. There was no staining in the control group. On the other hand, the degree of staining was markedly reduced in tissue sections by morphine. These results suggest that previous administration of subcutaneous morphine has preventive effect on ANTU-induced pulmonary inflammatory reaction and its effect mediated via peripheral opioid receptors. Application of naloxone with ANTU has no effect on the lung parameters indicating that endogenous opioids do not modulate ANTU-induced damage.
authors.affiliation: Department of General Surgery, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Comert
authors.forename: Emine Yilmaz
authors.initials: EY
authors.lastname: Sipahi
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ustun
authors.forename: Fulden
authors.initials: F
authors.lastname: Isikdemir
authors.forename: Gamze
authors.initials: G
authors.lastname: Numanoglu
authors.forename: Figen
authors.initials: F
authors.lastname: Barut
authors.forename: Hanife
authors.initials: H
authors.lastname: Altunkaya
authors.forename: Yetkin
authors.initials: Y
authors.lastname: Ozer
authors.forename: Ferruh
authors.initials: F
authors.lastname: Niyazi Ayoglu
authors.forename: Tunc Hakan
authors.initials: TH
authors.lastname: Sipahi
authors.forename: Ishak Ozel
authors.initials: IO
authors.lastname: Tekin
authors.forename: Z Nur
authors.initials: ZN
authors.lastname: Banoglu
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000031:analogs & derivatives* / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D011654:Pulmonary Edema / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D000644:Quaternary Ammonium Compounds; D051381:Rats; D013890:Thiourea / Q000031:analogs & derivatives* / Q000633:toxicity
chemical_list: D000644:Quaternary Ammonium Compounds; D009270:Naloxone; C041269:N-methylnaloxone; D009020:Morphine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013890:Thiourea; C005277:alpha-naphthyl thiourea