Inducing P-selectin ligand formation in CD8 T cells: IL-2 and IL-12 are active in vitro but not required in vivo.
In vitro studies have demonstrated that IL-2 and IL-12 can support formation of P-selectin ligands (P-SelL) in activated T cells, ligands that are variably required for efficient lymphocyte recruitment to sites of inflammation. To ascertain whether these cytokines were required for P-SelL formation in vivo, TCR transgenic CD8 T cells specific for male Ag (HY) were transferred into male mice under conditions in which either IL-2 and/or IL-15 or IL-12Rp40 were absent. P-SelL formation at day 2 was unperturbed in HY-TCR IL-2(null) CD8 T cells responding in doubly deficient IL-2(null)IL-12(null) or IL-2(null)IL-15(null) male recipients. HY-specific CD8 T cell proliferative responses detected in both spleen and peritoneum occurred vigorously, but only splenic CD8 T cells up-regulated P-SelL, demonstrating that in vivo induction of P-SelL is an active, nonprogrammed event following T cell activation and that despite the efficacy of IL-2 and IL-12 in supporting P-SelL formation in vitro, these cytokines appear to be dispensable for this purpose in vivo.
Carlow
Douglas A
DA
Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada. doug@brc.ubc.ca
Williams
Michael J
MJ
Ziltener
Hermann J
HJ
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D049109:Cell Proliferation; D006182:H-Y Antigen / Q000276:immunology; D018664:Interleukin-12 / Q000502:physiology*; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2 / Q000502:physiology*; D017710:Lymphocyte Transfusion; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D021122:Protein Subunits / Q000502:physiology*; D013154:Spleen / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects
D006182:H-Y Antigen; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D021122:Protein Subunits; D018664:Interleukin-12