title: Localization of the Drosophila protein FL(2)D in somatic cells and female gonads.
abstract: The Drosophila gene female-lethal(2)d [fl(2)d] has been implicated in the alternative splicing regulation of genes involved in sexual determination, such as Sex-lethal and transformer, and in the alternative splicing of the gene Ultrabithorax. Here, the expression and subcellular localization of the FL(2)D protein is reported. FL(2)D is ubiquitously expressed in embryos, in imaginal discs of larvae and in ovaries. In Schneider cells, the protein is detected throughout the nucleoplasm, excluding the nucleolus, and partially co-localizes with SXL in nuclear speckles. In addition, FL(2)D associates with multiple transcriptionally active loci on salivary gland polytene chromosomes, consistent with the proposal that the protein is involved in the post-transcriptional regulation of a variety of genes. Interestingly, the localization of FL(2)D in nurse cells changes during oogenesis, from a rather diffuse early nuclear pattern in the germarium to a preferential accumulation at the nuclear periphery of nurse cells of developing cysts.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany. aortega@ugr.es
authors.forename: A
authors.initials: A
authors.lastname: Ortega
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004330:Drosophila / Q000166:cytology* / Q000196:embryology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes; D007211:Indoles; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000378:metabolism; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D012235:Rhodamines
chemical_list: D029721:Drosophila Proteins; D005456:Fluorescent Dyes; D007211:Indoles; D019476:Insect Proteins; D009687:Nuclear Proteins; D012235:Rhodamines; C410299:fl(2)d protein, Drosophila; C007293:DAPI; D016650:Fluorescein-5-isothiocyanate