title: Role of gap junctional intercellular communication (GJIC) through p38 and ERK1/2 pathway in the differentiation of rat neuronal stem cells.
abstract: Gap junctional intercellular communications (GJIC) contributes to neural function in development and differentiation of CNS. In this study, we have investigated the expression of GJIC during the differentiation of neuronal stem cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced neuronal stem cell-derived cells from rat brain. During neuronal stem cell differentiation, expressions of Cx43 and 32 were increased for the duration of 72 hr, however the effect were decreased on the 7d. In the neuronal stem cell-derived cells, pretreatments with p38 MAP kinase inhibitor, SB203580, and MEK inhibitor, PD98059, could protect GJIC against TPA-induced inhibition of GJIC. Our data suggest that GJIC plays an important role during neuronal stem cell differentiation, and ERK1/2 and p38 MAP kinase signaling pathway may be closely related functionally to regulate gap junction in rat neuronal stem cell-derived cells.
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Se-Ran
authors.initials: SR
authors.lastname: Yang
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho
authors.forename: Nam-Shik
authors.initials: NS
authors.lastname: Ahn
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Jung
authors.forename: Joon-Suk
authors.initials: JS
authors.lastname: Park
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Jo
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Hwang
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Jung
authors.forename: Tae-Yung
authors.initials: TY
authors.lastname: Kim
authors.forename: Byoung-Su
authors.initials: BS
authors.lastname: Yoon
authors.forename: Bong-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.forename: Yong-Soon
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D018031:Connexin 43 / Q000378:metabolism; D017630:Connexins / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D017629:Gap Junctions / Q000502:physiology*; D007093:Imidazoles / Q000494:pharmacology; D009474:Neurons / Q000166:cytology*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats / Q000196:embryology*; D013234:Stem Cells / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate; D000097002:Gap Junction beta-1 Protein
chemical_list: D018031:Connexin 43; D017630:Connexins; D005419:Flavonoids; D007093:Imidazoles; D011725:Pyridines; D013755:Tetradecanoylphorbol Acetate; D000097002:Gap Junction beta-1 Protein; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one