title: Characterization of the human T cell response to antigen 5 from Vespula vulgaris (Ves v 5).
abstract: BACKGROUND
  The T cell reactivity to the major allergen of bee venom, phospholipase A2, has been thoroughly characterized. In contrast, only little is known about the human cellular response to major allergens from wasp venom.
  
  
  OBJECTIVE
  To characterize the human T cell response to antigen 5 from Vespula vulgaris, Ves v 5.
  
  
  METHODS
  Recombinant Ves v 5 was used to establish allergen-specific T cell lines (TCL) and T cell clones (TCC) from the peripheral blood of vespid-allergic and non-allergic individuals. Ves v 5-specific TCL were mapped for T cell epitopes using overlapping synthetic peptides representing the complete amino acid sequence of Ves v 5. Ves v 5-specific TCC were analysed for antigen-induced secretion of IL-4, IFN-gamma and IL-10.
  
  
  RESULTS
  Seventeen distinct T cell epitopes were recognized by allergic individuals among which Ves v 5(181-192) was identified as a dominant T cell epitope. Partially different epitopes were observed in TCL from non-allergic subjects and the dominant epitope Ves v 5(181-192) was not prevalent in these cultures. Ves v 5-specific TCC isolated from allergic individuals did not show the typical T helper type 2 (Th2)-like cytokine profile in response to specific stimulation, i.e. high amounts of IL-4 and low IFN-gamma. TCC from non-allergic individuals showed a Th1-like cytokine pattern.
  
  
  CONCLUSIONS
  Our findings provide evidence that the allergic T cell response to Ves v 5 is not Th2-dominated and that different immunogenic sites on this major wasp venom allergen are recognized by allergic and non-allergic individuals.
authors.affiliation: Department of Pathophysiology, Medical University of Vienna, VA-1090 Vienna, Austria. barbara.bohle@meduniwien.ac.at
authors.forename: B
authors.initials: B
authors.lastname: Bohle
authors.forename: B
authors.initials: B
authors.lastname: Zwölfer
authors.forename: G F
authors.initials: GF
authors.lastname: Fischer
authors.forename: U
authors.initials: U
authors.lastname: Seppälä
authors.forename: T
authors.initials: T
authors.lastname: Kinaciyan
authors.forename: C
authors.initials: C
authors.lastname: Bolwig
authors.forename: M D
authors.initials: MD
authors.lastname: Spangfort
authors.forename: C
authors.initials: C
authors.lastname: Ebner
mesh_terms: D000485:Allergens / Q000276:immunology*; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D016022:Case-Control Studies; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014862:Wasp Venoms / Q000276:immunology*
chemical_list: D000485:Allergens; D000941:Antigens; D000939:Epitopes; D011994:Recombinant Proteins; C431905:Ves v 5 allergen; D014862:Wasp Venoms; D007073:Immunoglobulin E