Analysis of micronuclei in the transferrin-receptor positive reticulocytes from peripheral blood of nasopharyngeal cancer patients undergoing radiotherapy by a single-laser flow cytometer.
The automated micronucleus test is now accepted as a simple, objective, and accurate method for evaluating potential mutagenic effects caused by physical, chemical or biotic factors. This paper describes a single-laser flow cytometry, based on an immunomagnetic isolation technique in combination with acridine orange staining, to detect frequencies of micronucleated transferrin-receptor positive reticulocytes from human peripheral blood. Using this flow cytometric system, we detected the frequencies of micronucleated transferrin-receptor positive reticulocytes from 10 nasopharyngeal cancer patients undergoing radiotherapy and the baseline of the frequencies of micronucleated transferrin-receptor positive reticulocytes from 7 healthy donors. The results showed that the mean frequency of micronucleated transferrin-receptor positive reticulocytes from healthy donors was 0.236% and that from nasopharyngeal cancer patients before radiotherapy was 0.297%. After radiotherapy it was significantly elevated. When the cumulative dose of radiotherapy was about 20Gy, it reached a maximum of 6.905%, and then, as the cumulative dose of radiotherapy continued to increase to 30Gy, 40Gy and 50Gy, the frequency decreased to 6.258%, 5.119% and 5.007% respectively. Our results indicated that the single-laser flow cytometric system was quick, reasonable and acceptable for detecting the frequency of micronucleated transferrin-receptor positive reticulocytes from human peripheral blood.
Sun
Li Ping
LP
Department of Hygiene Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, Peoples Republic of China.
Li
De Zhi
DZ
Liu
Zhi Mou
ZM
Yang
Lu Jun
LJ
Liu
Jin Yi
JY
Cao
Jia
J
D000328:Adult; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D047569:Laser Scanning Cytometry / Q000379:methods*; D015162:Micronucleus Tests / Q000379:methods*; D009302:Nasopharyngeal Diseases / Q000097:blood / Q000532:radiotherapy; D011832:Radiation Injuries / Q000097:blood* / Q000209:etiology*; D011879:Radiotherapy Dosage; D011990:Receptors, Transferrin / Q000097:blood*; D015203:Reproducibility of Results; D012156:Reticulocytes / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
D011990:Receptors, Transferrin