Neutrophil chemokines in epithelial inflammatory processes of human tonsils.
CXC chemokines are thought to play an important role at sites of inflammation. Because ELR(+) CXC chemokines are expressed in different types of tonsillitis we investigated the role of the surface/crypt epithelium of human tonsils in producing ELR(+) CXC chemokines: interleukin (IL)-8 (CXCL8), ENA-78 (CXCL5), GRO-alpha (CXCL1) and GCP-2 (CXCL6). Tonsillar tissue was obtained from patients undergoing tonsillectomy and chemokine expression was investigated by means of immunohistochemistry. A549 cells were used as a model to study kinetics of chemokine expression in epithelial cells. Cells were stimulated with tumour necrosis factor (TNF)-alpha, lipopolysaccharide (LPS) and supernatants derived from aerobic/anaerobic Staphylococcus aureus strains. Chemokine expression was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). We observed epithelial expression of IL-8, GRO-alpha and GCP-2 in different types of tonsillitis, whereas ENA-78 was rarely expressed. In A549 cells abundant expression of ENA-78 was detected. IL-8 and GCP-2 are expressed in an acute type of tonsillitis whereas GRO-alpha was frequently detectable both in chronically and acutely inflamed tonsils. ENA-78 does not seem to play a pivotal role in tonsillitis in vivo.
Sachse
F
F
Department of Otolaryngology, Head and Neck Surgery, University of Münster,Kardinal-von-Galen-Ring 10, D-48149 Münster, Germany. sachsef@mednet.uni-muenster.de
Ahlers
F
F
Stoll
W
W
Rudack
C
C
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D054360:Chemokine CXCL1; D054427:Chemokine CXCL6; D018925:Chemokines / Q000378:metabolism*; D019743:Chemokines, CXC / Q000378:metabolism; D002648:Child; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013211:Staphylococcus aureus / Q000276:immunology; D014069:Tonsillitis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
C475829:CXCL1 protein, human; C515503:CXCL6 protein, human; D054360:Chemokine CXCL1; D054427:Chemokine CXCL6; D018925:Chemokines; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha