title: Antioxidant activity of isoacteoside from Clerodendron trichotomum.
abstract: The antioxidant properties of isoacteoside, isolated from Clerodendron trichotomum (Verbenaceae), were investigated. This compound scavenged intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. This radical scavenging activity of isoacteoside protected cell viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2). Furthermore, isoacteoside reduced the apoptotic cells formation induced by H2O2, as demonstrated by the decreased number of sub-G1 hypo-diploid cells and apoptotic cell body formation. However, isoacteoside increased the activities of cellular antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Taken together, these findings suggest that isoacteoside, isolated from C. trichotomum, possesses antioxidant properties.
authors.affiliation: Natural Products Research Institute and College of Pharmacy, Seoul National University, Seoul, Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.forename: Ju Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Hyung Deuk
authors.initials: HD
authors.lastname: Bu
authors.forename: Youngki
authors.initials: Y
authors.lastname: Lee
authors.forename: Young Rok
authors.initials: YR
authors.lastname: Seo
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D002374:Catalase / Q000096:biosynthesis / Q000494:pharmacology; D018929:Cell Culture Techniques; D032409:Clerodendrum / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins; D005347:Fibroblasts; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000032:analysis; D015227:Lipid Peroxidation; D008168:Lung / Q000166:cytology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000494:pharmacology; D029648:Verbenaceae
chemical_list: D000975:Antioxidants; D003603:Cytotoxins; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C064683:isoacteoside; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase