Alternative nucleic acid analogues for programmable assembly: hybridization of LNA to PNA.
Complementary locked nucleic acid (LNA) and peptide nucleic acid (PNA) hexamers bind to each other with significantly higher affinity than each binds to DNA, and with far greater affinity than DNA binds to complementary DNA. The hybridization is highly specific with a single mismatch causing decreases in T(m) values ranging from 12 (G/T) to 30 degrees C (A/A). Importantly, the hybridization of an LNA oligomer to a PNA oligomer is unaffected by the ionic strength of the buffer. These properties make the LNA/PNA pair an attractive candidate as a replacement for DNA in programmable assembly.
Ng
Pei-Sze
PS
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Buildings, Purdue University, 201 South University Street, West Lafayette, Indiana 47907-2064, USA.
Bergstrom
Donald E
DE
D002021:Buffers; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D009994:Osmolar Concentration; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011119:Polynucleotides / Q000737:chemistry*
D002021:Buffers; D037742:Nanotubes, Carbon; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D011119:Polynucleotides; C477371:locked nucleic acid; D004247:DNA