title: Liquid chromatographic method for quantifying polyphenols in ciders by direct injection.
abstract: An analytical method for the quantitative determination of the principal phenolic compounds (benzoic acids, hydroxycinnamic acids, 3-phenylpropionic acids, flavanols, procyanidins, dihydrochalcones, quercetin glycosides) in ciders, which successfully employs a RP-HPLC and photodiode-array detection system without prior treatment of the sample, is described. Parameters usually examined in the method validation were evaluated. Good linearity was obtained with correlation coefficients exceeding 0.999 and the detection limits ranged from 0.07 mg/L (p-hydroxybenzoic acid) to 2 mg/L (hydrocaffeic acid). Recoveries ranging between 90 and 104% and the reproducibility of the method was always < 8% (RSD). The method was applied to a set of commercial samples and the results obtained may be helpful to establish a phenolic profile in Asturian cider.
authors.affiliation: Area de Tecnología de los Alimentos, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), 33300-Villaviciosa, Asturias, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Suárez
authors.forename: Noemí
authors.initials: N
authors.lastname: Palacios
authors.forename: Natalia
authors.initials: N
authors.lastname: Fraga
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D015203:Reproducibility of Results; D013030:Spain
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols