title: In vitro genotoxic effects of the anticancer drug gemcitabine in human lymphocytes.
abstract: This research was carried out to investigate in vitro genotoxic effects of the anticancer agent gemcitabine on the induction of chromosomal aberrations and sister-chromatid exchange in human lymphocytes. Three doses of gemcitabine (0.001, 0.002 and 0.004 microg/ml) were applied to lymphocyte cultures from 15 donors. There was a significant increase in the induction of chromosome aberrations and in the occurrence of sister-chromatid exchange in these cells. In addition, gemcitabine significantly decreased the mitotic index and replicative index for all doses. Dose-response regression lines were used to compare the individual susceptibilities to gemcitabine with respect to the chromosome aberration and sister-chromatid exchange frequencies. Our results indicate that gemcitabine is able to induce both cytotoxic and genotoxic effects in human lymphocyte cultures in vitro in a dose-dependent manner.
authors.affiliation: Uludag University, Faculty of Arts and Sciences, Department of Biology, Gorukle, Bursa 16059, Turkey. aydemirn@uludag.edu.tr
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Aydemir
authors.forename: Serap
authors.initials: S
authors.lastname: Celikler
authors.forename: Rahmi
authors.initials: R
authors.lastname: Bilaloğlu
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D012854:Sister Chromatid Exchange; D000093542:Gemcitabine
chemical_list: D000970:Antineoplastic Agents; D003841:Deoxycytidine; D000093542:Gemcitabine