Influences of DTC and zinc supplementation on the cellular response restoration in restrained mice.
The studies were conducted on Balb/c mice exposed to restraint stress twice for 12 h at 24 h intervals. Prior to restraint stress the mice were treated with sodium diethyldithiocarbamate (DTC) i.p. at a dose of 20 mg/kg five times at 48 h intervals. DTC was used per se or with zinc ions interaction, by adding zinc sulfate to drinking water at a dose of 72 microgram/mouse daily. The results obtained in the study show that restraint stress causes involution of lymphatic organs, decreased the percentage of immature (CD4+CD8+) and, mature (CD4+) thymocytes and CD4+), CD8+ and CD19+ splenocytes and proliferative response of thymocytes stimulated in vitro with concanavalin A (Con A) and phytohemagglutinin (PHA). The restraint stress decreased also interleukin-1 (IL-1) production by murine intraperitoneal macrophages stimulated in vitro with lipopolysaccharide (LPS) from E. coli. Pretreatment with DTC counteracted restraint stress-induced immunosuppression, which is expressed as partial normalisation of the total number of thymocytes, splenocytes and IL-1 production, accelerated regeneration of thymus and spleen, shorter suppressive action of restraint stress on the percentage of CD4+CD8+thymocytes and in total normalisation of the CD4+thymocytes and splenocytes. DTC administered prior to restraint stress augmented the proliferative response of thymocytes to two mitogens. The immunocorrecting action of DTC is enhanced by zinc supplementation, expressed in the increased percentage of CD4+thymocytes and splenocytes, CD19+splenocytes, proliferative activity of thymocytes stimulated with PHA and IL-1 production. The obtained results show that DTC administration can be supplemented with zinc in order to restore the immune system impaired by stress.
Obminska-Mrukowicz
Bozena
B
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agricultural University, Norwida 31, 50-375 Wroclaw, Poland. m.mrukowicz@triangulum.pl
Szczypka
Marianna
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004050:Ditiocarb / Q000494:pharmacology*; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007375:Interleukin-1 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000096:biosynthesis; D009929:Organ Size / Q000187:drug effects; D012149:Restraint, Physical; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013312:Stress, Physiological / Q000209:etiology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D019287:Zinc Sulfate / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D008934:Mitogens; D019287:Zinc Sulfate; D004050:Ditiocarb