A sensitive liquid chromatography-tandem mass spectrometry method for the quantification of mometasone furoate in human plasma.
A robust, rapid, selective and sensitive liquid chromatography-negative atmospheric pressure chemical ionization (LC-(APCI(-))-MS-MS) method has been developed for the quantification of mometasone furoate (MF) in human plasma utilizing a solid-phase extraction clean-up step and 13C-fluticasone propionate as internal standard. The intra- and inter-day coefficients of variation were < or = 15% and the lower limit of quantification (LLOQ) was 15 pg/ml. This method is ideally suited for pharmacokinetic investigations of low MF levels following inhalation of MF.
Sahasranaman
Srikumar
S
Department of Pharmaceutics, College of Pharmacy, PO 100494, University of Florida, Gainesville, FL 32610, USA.
Tang
Yufei
Y
Biniasz
Diana
D
Hochhaus
Guenther
G
D000893:Anti-Inflammatory Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D000068656:Mometasone Furoate; D011244:Pregnadienediols / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000893:Anti-Inflammatory Agents; D011244:Pregnadienediols; D000068656:Mometasone Furoate