The influence of prolactin on the chosen biochemical parameters of the rabbit liver in ischemia.
An experimental model, based on Pringle's scheme of acute warm hepatic ischemia in normothermia was employed in order to study the hepatoprotective properties of prolactin (PRL). In the proposed model one liver lobe was maintained in the portal circulation and the remaining lobes were perfused with HTK solution for 2 hours. The experiment was carried out on female rabbits of the Chinchilla race. In the control group (n= 10) the liver was perfused with HTK solution. In the examined group (n=10), 3 microg of PRL per g of liver per hour was added to HTK solution. Additionally, the animals in the PRL-treated group were intravenously administered a dose of 600 microg of PRL / kg body weight. 1 h before the surgical treatment. The activities of alanine aminotransferase (ALT), alkaline phosphatase (ALP). gamma-glutamyl transferase (GGT) and the lactate concentration were determined in the eluate obtained from the perfused part of the liver. It was found that administration of prolactin during 2 h of perfusion led to a significant decrease of ALT, ALP and lactate concentrations in the eluate. In addition, increase of calcium concentration in the liver was significantly lower with the prolactin group. The observed results let us to draw the conclusion that administration of PRL shows signs of protective effects on hepatocytes in normothermic acute ischemia.
Szulc-Musioł
Beata
B
Department of Applied Pharmacy and Drug Technology, Silesian Academy of Medicine, Poland.
Drózdz
M
M
Ryszka
F
F
Dolínska
B
B
Scigała
P
P
D000818:Animals; D002682:Chinchilla; D005260:Female; D066298:In Vitro Techniques; D007511:Ischemia; D008102:Liver Circulation / Q000187:drug effects / Q000502:physiology*; D008107:Liver Diseases / Q000201:enzymology / Q000517:prevention & control*; D008111:Liver Function Tests; D011388:Prolactin / Q000627:therapeutic use*; D011817:Rabbits; D013552:Swine
D011388:Prolactin