Cell cycle profiles of EcR, USP, HR3 and B cyclin mRNAs associated to 20E-induced G2 arrest of Plodia interpunctella imaginal wing cells.
Using the IAL-PID2 cell line established from pupally committed imaginal wing discs of Plodia interpunctella, we have investigated the dynamics of cellular and molecular events involved in the G2/M arrest. We have first cloned a cDNA sequence named PIUSP-2 that likely encodes a homologue of the Ultraspiracle-2 isoform of Manduca sexta. When the IAL-PID2 cells were exposed to a 8 h 20E treatment applied at different times of the cell cycle, an optimal period of sensitivity of cells to 20E, in inducing G2 arrest, was determined at the S/G2 transition. Using cDNA probes specifically designed from Plodia B cyclin (PcycB), ecdysone receptor B1-isoform (PIEcR-B1) and HR3 transcription factor (PHR3), we provide evidence that the 20E-induced G2 arrest was correlated to a high induction of PHR3, PIEcR-B1, PIUSP-2 mRNAs at the S/G2 transition and a decrease in PcycB mRNA level at the end of G2 phase.
Siaussat
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Laboratoire de Physiologie Cellulaire des Invertébrés, Université Pierre et Marie Curie, Paris, France. David.Siaussat@snv.jussieu.fr
Bozzolan
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Queguiner
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Porcheron
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Debernard
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D000595:Amino Acid Sequence; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D019926:Cyclin B / Q000378:metabolism; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins; D004441:Ecdysterone / Q000494:pharmacology; D016195:G2 Phase / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D019105:Random Amplified Polymorphic DNA Technique; D011987:Receptors, Steroid / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000378:metabolism*
D019926:Cyclin B; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D012333:RNA, Messenger; D011987:Receptors, Steroid; D014157:Transcription Factors; C066030:USP protein, Drosophila; C055469:ecdysone receptor; D004441:Ecdysterone; D000841:Anisomycin