Breeding genetically modified rodents: tips for tracking and troubleshooting reproductive performance.
Infertility or reduced fertility in genetically modified mouse strains represents a serious bottleneck for planned research projects. The authors describe simple methods for troubleshooting poor reproductive performance in breeding colonies and introduce various means of assisted reproduction that might be used to 'rescue' infertile lines.
Murray
Kathleen A
KA
Charles River Laboratories, Transgenic Services, Wilmington, MA 01887, USA. kmurray@criver.com
Parker
Nancy Jean
NJ
D000822:Animal Husbandry; D000818:Animals; D030801:Animals, Genetically Modified; D001947:Breeding / Q000379:methods*; D005260:Female; D007246:Infertility / Q000235:genetics*; D008297:Male; D051379:Mice; D012098:Reproduction / Q000235:genetics*; D027724:Reproductive Techniques, Assisted / Q000662:veterinary; D014730:Veterinary Medicine / Q000379:methods*