Production of heterogeneous dimer lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli cells.
The lignostilbenedioxygenase isozyme I and III genes, lsdA and lsdB, were coexpressed within the same Escherichia coli cells. The lignostilbenedioxygenase isozymes produced were separated on QAE anion-exchange column chromatography. Three parts of active fractions corresponding to alphaalpha, alphabeta, and betabeta dimers were detected. The purified isozyme from second active fractions corresponded to the native heterogeneous isozyme II.
Kamoda
Shigehiro
S
University Forest in Hokkaido, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Hokkaido, Japan. kamo@uf.a.u-tokyo.ac.jp
Terada
Tamami
T
Saburi
Yoshimasa
Y
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019281:Dimerization; D049308:Dioxygenases / Q000096:biosynthesis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial
D049308:Dioxygenases; C069684:lignostilbene-alpha,beta-dioxygenase