DNA extraction from bovine mummified fetuses and detection of factor XI gene deficiency in the mummies.
Genomic DNA extracted from bovine mummified tissue is valuable material for detection of some genes that may contribute to fetal abnormalities. In this study bovine genomic DNA was extracted from the hardened tissue samples of ten bovine mummified fetuses. The amount of genomic DNA extracted from 2 g of the mummified tissues by the phenol/chloroform-ethanol method was low (less than 4 microg/ml) for all samples. The extracted DNA was then amplified by the GenomiPhi DNA amplification system. After amplification, the amount of DNA was increased to more than 100 microg/ml for all samples. This amplification system was shown to be a good tool for amplifying the genomic DNA of the mummified fetuses. The amplified genomic DNA was used for testing the mummies for Factor XI gene deficiency, an autosomal recessive deficiency involved in the early stages of the intrinsic blood coagulation pathway. Exon 12 of the Factor XI gene of the mummies was amplified by PCR. Two of the ten mummified fetuses were heterozygous for the Factor XI gene as indicated by the presence of two amplified DNA fragments of 320 bp and 244 bp. Factor XI deficiency has already been described in Holstein cattle. However, no report is available for bovine fetus. In this study, DNA was extracted and amplified from the bovine mummified fetuses, and the samples were successfully tested for Factor XI gene deficiency in the mummies.
Ghanem
Mohamed Elshabrawy
ME
Laboratory of Animal Science, Graduate School for International Development and Cooperation, Hiroshima University, Japan.
Nishibori
Masahide
M
Nakao
Toshihiko
T
Moriyoshi
Masaharu
M
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000196:embryology / Q000235:genetics* / Q000473:pathology; D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting; D005172:Factor XI / Q000235:genetics*; D005173:Factor XI Deficiency / Q000235:genetics / Q000662:veterinary*; D005333:Fetus / Q000473:pathology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA
D004247:DNA; D005172:Factor XI