Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the quantitation of levodropropizine in human plasma.
A rapid and sensitive LC-MS-MS method for quantifying levodropropizine in human plasma after oral administration of a single-dose (60 mg/day) was developed and validated. The sample preparation used liquid-liquid extraction with a mixture of dichloromethane-diethyl ether (2:3, v/v) in a basic environment. The retention time of levodropropizne and zolmitriptan (used as internal standard) was 1.6 and 1.4 min, respectively. The assay was linear over the range 0.25-500 ng/mL with a LOQ of 0.25 ng/mL. The intra- and inter-day precision were < 8.1% and < 11.5%, respectively, and the accuracy was in the range 87.6-112%. The levodropropizine concentration profile in human plasma was determined.
Tang
Yunbiao
Y
Research Center for Drug Metabolism, Jilin University, Jiefang Road 123, Changchun 130023, China.
Zhao
Limei
L
Wang
Yingwu
Y
Fawcett
J Paul
JP
Gu
Jingkai
J
D000996:Antitussive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011409:Propylene Glycols / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000996:Antitussive Agents; D011409:Propylene Glycols; C035916:dipropizine