Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional analysis in yeast cells.
Trehalose is a nonreducing disaccharide composed of two glucose units joined by an alpha, alpha-1, 1 linkage, and has been found in bacteria, yeast, fungi, invertebrates and plants. Accumulation of trehalose in organisms plays a role in enhancing the stress tolerance. A trehalose-6-phosphate phosphatase gene, NtTPPL, was isolated from tobacco in this report. The predicted NtTPPL protein has a putative trehalose_PPase domain. The transcription of the NtTPPL gene was significantly induced by heat stress, and was only slightly induced by NaCl, PEG and low-temperature treatments. When expressing in yeast tps2 mutant, NtTPPL rescued the mutant phenotype under high temperature. This result indicated that NtTPPL functioned as a trehalose-6-phosphate phosphatase in yeast and may play similar roles in plants.
Wang
Yu-Jun
YJ
National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100101, China.
Hao
Yu-Jun
YJ
Zhang
Zhi-Gang
ZG
Chen
Tao
T
Zhang
Jin-Song
JS
Chen
Shou-Yi
SY
D000595:Amino Acid Sequence; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D030781:Organisms, Genetically Modified; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics*
C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases