Immunohistochemical evaluation of periprosthetic membrane from loose cemented and uncemented total hip arthroplasty.
OBJECTIVE
Evaluate the cellular mechanisms responsible for the aseptic loosening of total hip replacements.


METHODS
Twenty periprosthetic membranes were collected during revision procedures at the Department of Orthopedics, Hacettepe University, Ankara, Turkey, during 1998 to 1999. Arthroplasties with polyethylene components and without polyethylene components were examined under light microscopy and immunohistochemically using the indirect immunoperoxidase technique into 2 different groups.


RESULTS
Immunohistochemical analysis with monoclonal antibodies reactive to certain cell surface antigens (CD45, CD44, CD98, CD31, CD26, CD71) revealed different morphological characteristics regarding the adjacent fibrosis, leukocyte activation, and immunologic response.


CONCLUSIONS
These findings suggested the role of different cellular mechanisms in each group.
Atilla
Bulent
B
Department of Orthopedics and Traumatology, Faculty of Medicine, Hacettepe University, Samanpazari 06100, Ankara, Turkey. b-atilla@hacettepe.edu.tr
Atilla
Pergin
P
Tokgözoglu
Ahmet M
AM
Cakar
Ayse N
AN
Alpaslan
Ahmet M
AM
D015703:Antigens, CD / Q000378:metabolism; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects*; D001843:Bone Cements; D003094:Collagen / Q000648:ultrastructure; D006622:Hip Prosthesis; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology; D011475:Prosthesis Failure; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
D015703:Antigens, CD; D001843:Bone Cements; D003094:Collagen