DNA-functionalized MFe2O4 (M = Fe, Co, or Mn) nanoparticles and their hybridization to DNA-functionalized surfaces.
Magnetic MFe2O4 (M = Fe, Co, or Mn) nanoparticles with uniform diameters in the 4-20 nm range and with excellent material properties, reported previously, can be rendered soluble in water or aqueous buffers using a combination of alkylphosphonate surfactants and other surfactants such as ethoxylated fatty alcohols or phospholipids. Surfactant-modified oligonucleotides can be incorporated into the particles' organic shell. The particles can withstand salt concentrations up to 0.3 M, temperatures up to 90 degrees C, and various operations such as concentration to dryness, column or membrane separations, and electrophoresis. The particles can be selectively hybridized to DNA-functionalized gold surfaces with high coverages using a two-story monolayer structure. These particles may find valuable applications involving the magnetic detection of small numbers of biomolecules using spin valves, magnetic tunnel junctions, or other sensors.
Robinson
David B
DB
IBM T. J. Watson Research Center, Yorktown Heights, New York 10598, USA.
Persson
Henrik H J
HH
Zeng
Hao
H
Li
Guanxiong
G
Pourmand
Nader
N
Sun
Shouheng
S
Wang
Shan X
SX
D000478:Alkylation; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D006046:Gold; D007501:Iron / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D063065:Organophosphonates / Q000737:chemistry; D013057:Spectrum Analysis; D013499:Surface Properties; D013696:Temperature
D015342:DNA Probes; D063065:Organophosphonates; D003035:Cobalt; D008345:Manganese; D006046:Gold; D004247:DNA; D007501:Iron