title: Serum antioxidant activities, malondialdehyde and nitric oxide levels in human cutaneous leishmaniasis.
abstract: Leishmania sp. are obligate intracellular protozoa that infect and replicate within mammalian macrophages. Macrophages, neutrophils and other phagocytic cells are key components of the antimicrobial and tumoricidal immune responses. These cells are capable of generating large amounts of reactive oxygen species (ROS) and reactive nitrogen species (RNS). To examine antioxidant status and lipid peroxidation in cutaneous leishmaniasis (CL) patients, activities of two ROS scavenging enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the levels of malondialdehyde (MDA) and nitric oxide (NO(.)) have been studied in serum. Blood samples were taken from CL patients before treatment (n = 27) and after the treatment (n = 18). NO(.) and MDA levels, SOD and GSH-Px activities were compared between untreated and treated CL patients and control subjects (n = 23). There was a significant decrease in SOD and GSH-Px activities in the CL patients (P < 0.0001). Significantly higher levels of serum MDA and NO(.) levels were found in CL patients, compared to controls and treated patients. It may be suggested that the overproduction of ROS and RNS results in oxidative stress and the acceleration of lipid peroxidation in CL patients, resulting from altered enzymatic antioxidant activities.
authors.affiliation: Department of Dermatology, Mustafa Kemal University, Faculty of Medicine, Hatay, Turkey. gserarslan@hotmail.com
authors.forename: G
authors.initials: G
authors.lastname: Serarslan
authors.forename: H R
authors.initials: HR
authors.lastname: Yilmaz
authors.forename: S
authors.initials: S
authors.lastname: Söğüt
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000097:blood* / Q000188:drug therapy; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008536:Meglumine / Q000627:therapeutic use; D000077485:Meglumine Antimoniate; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D009942:Organometallic Compounds / Q000627:therapeutic use; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D000981:Antiprotozoal Agents; D009942:Organometallic Compounds; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D008536:Meglumine; D000077485:Meglumine Antimoniate; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase