title: cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients.
abstract: Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with development of peptic ulcer disease and gastric malignancies. The vacuolating cytotoxin (vacA), cagA gene, and babA2 gene are important virulence factor involving gastric diseases. Eighty-nine Helicobacter pylori-positive gastric biopsies were analyzed by polymerase chain reaction and Southern blotting for H. pylori detection and genotyping with primer pairs from each virulence gene. Fifty-three strains (59%) were common vacA genotype s1/m1, and only 14 (16%) were s2/m2, 12% of strains was found to have multiple infection. The cagA presence was detected in 48% (43 strains) and babA2 gene was detected in 44% of our H. pylori strains. We observed high percentage of s1/m1 strains with chronic gastritis and peptic ulcer and a significant correlation between cagA presence with the s1 allele and babA2 gene with chronic gastritis.
authors.affiliation: Faculdade de Medicina de Marília (FAMEMA), Marília, São Paulo 17519-050, Brazil. lobogatti@yahoo.com.br
authors.forename: Luciano Lobo
authors.initials: LL
authors.lastname: Gatti
authors.forename: Ellen Kris
authors.initials: EK
authors.lastname: Fagundes e Souza
authors.forename: Kátia Ramos
authors.initials: KR
authors.lastname: Leite
authors.forename: Eduardo Lemos de Souza
authors.initials: EL
authors.lastname: Bastos
authors.forename: Luciano Roberto
authors.initials: LR
authors.lastname: Vicentini
authors.forename: Luiz Carlos da
authors.initials: LC
authors.lastname: Silva
authors.forename: Marília de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
authors.forename: Spencer Luiz Marques
authors.initials: SL
authors.lastname: Payão
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000483:Alleles; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D005756:Gastritis / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D010437:Peptic Ulcer / Q000382:microbiology; D016133:Polymerase Chain Reaction
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; C400429:BabA protein, Helicobacter pylori; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori