Anti-tumor activities of four chelating agents against human neuroblastoma cells.
BACKGROUND
Iron deprivation may be a therapeutic strategy for cancer. It can be achieved by using iron chelators. In this investigation, anti-neuroblastoma activities of a novel ferric chelator 2LL together with DFO, EDTA and DTPA were evaluated.


METHODS
SH-Sy5y cells were cultured at 37 degrees C in 5% CO2/95% air in DMEM containing 10% fetal bovine serum. The cells were seeded in 96-well microtiter plates overnight. Then, chelating agents were added into the wells. After 48-hour incubation, viabilities were measured using the MTT method.


RESULTS
DTPA had an IC50 value between 60-100 microM; DFO produced about 40% inhibiting effect at 150 microM; 2LL and EDTA displayed about 10% inhibiting effect at high concentrations.


CONCLUSIONS
For SH-Sy5y cells, DTPA showed the strongest inhibiting effect, DFO displayed a moderate inhibiting effect, while 2LL and EDTA produced minor inhibition. To develop iron chelators as powerful anti-cancer agents is still a challenging task.
Shen
Langtao
L
National Isotope Center of Engineering and Technology, China Institute of Atomic Energy, Beijing 102413, China. shenlt@yahoo.com
Zhao
Hui-Yun
HY
Du
Jin
J
Wang
Fan
F
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology*; D009447:Neuroblastoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013329:Structure-Activity Relationship; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D007502:Iron Chelating Agents