Morphine modulates inducible nitric oxide synthase expression and reduces pulmonary oedema induced by alpha-naphthylthiourea.
This study was designed to investigate the possible participation of morphine in pulmonary oedema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent in the lung. Injection of ANTU (15 mg/kg i.p.) produced pulmonary oedema as indicated by an increase in lung weight/body weight ratio and pleural effusion reaching a maximum within 4 h in rat. Administration of morphine prior to ANTU significantly inhibited to pulmonary oedema with a dose-dependent manner. The protective effect of morphine is prevented by peripheral opioid receptor antagonist, naloxone methiodide. ANTU-treated rats were shown positive by inducible nitric oxide synthase immunohistochemical staining. There was no staining in the control group. On the other hand, the degree of staining was markedly reduced in tissue sections by morphine. These results suggest that previous administration of subcutaneous morphine has preventive effect on ANTU-induced pulmonary inflammatory reaction and its effect mediated via peripheral opioid receptors. Application of naloxone with ANTU has no effect on the lung parameters indicating that endogenous opioids do not modulate ANTU-induced damage.
Comert
Mustafa
M
Department of General Surgery, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey.
Sipahi
Emine Yilmaz
EY
Ustun
Huseyin
H
Isikdemir
Fulden
F
Numanoglu
Gamze
G
Barut
Figen
F
Altunkaya
Hanife
H
Ozer
Yetkin
Y
Niyazi Ayoglu
Ferruh
F
Sipahi
Tunc Hakan
TH
Tekin
Ishak Ozel
IO
Banoglu
Z Nur
ZN
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000031:analogs & derivatives* / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D011654:Pulmonary Edema / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D000644:Quaternary Ammonium Compounds; D051381:Rats; D013890:Thiourea / Q000031:analogs & derivatives* / Q000633:toxicity
D000644:Quaternary Ammonium Compounds; D009270:Naloxone; C041269:N-methylnaloxone; D009020:Morphine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013890:Thiourea; C005277:alpha-naphthyl thiourea