Coulter counter use in the enumeration of muscle and fat stem cells.
Although the manual counting chamber (hemacytometer) is the gold standard for counting cells, this method is subject to great variability due to the 'human factor'. The automated cell counter (Coulter Counter) can enumerate cells in less time and with greater accuracy than the hemacytometer by removing many of the steps in which errors are made. While the Coulter Counter (and others of its type) has been used for many years in the cell culture field, there have been few studies to validate its use with specific cell types. We conducted several experiments in which we assessed the accuracy of the Coulter Counter over counts made with a hemacytometer as well as validated its use for the counting of satellite cells and preadipocytes.
Fernyhough
Melinda E
ME
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Helterline
Deri L
DL
Vierck
Janet L
JL
Hill
Rod A
RA
Dodson
Michael V
MV
D017667:Adipocytes / Q000166:cytology*; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D009132:Muscles / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*