Up-regulation of beta-catenin in external auditory canal cholesteatoma.
The external auditory canal cholesteatoma (EACC) is a rare disease with hyperproliferation and destructive growth in the adjacent structures. Down-regulation of beta-catenin (key component of the zonula adherens) is a pivotal factor for loose tissue integrity and invasiveness. Transforming growth factor beta1 (TGF-beta1) was reported to decrease beta-catenin in mammary epithelium. We investigated the abrogation of TGF-beta1 and beta-catenin expression in EACC culture cells. Cultured EACC-specimens were incubated with 6 micromol TGF-beta1 antisense. After 48 h, expression of beta-catenin was determined by means of immunohistochemistry. The cells showed an increased mural reactivity to beta-catenin, and intracellular reactivity was unchanged. The untreated cells showed a loss of beta-catenin expression at the membranes. The predominant membranous location after treatment with TGF-beta1 antisense suggests increased tendency of the cells for tissue formation and strong cell-cell adhesion rather than migratory and invasive character, and thus TGF-beta1 antisense application is a useful therapeutical strategy.
Naim
Ramin
R
Universitat-HNO-Klinik, Theodor-Kutzer-Ufer, 68135 Mannheim, Germany. ramin.naim@hno.ma.uni-heidelberg.de
Chang
Ray C
RC
Anders
Clemens
C
Sadick
Haneen
H
Riedel
Frank
F
Bayerl
Christiane
C
Bran
Gregor
G
Hormann
Karl
K
D002478:Cells, Cultured; D002781:Cholesteatoma / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004424:Ear Canal / Q000378:metabolism*; D004427:Ear Diseases / Q000378:metabolism; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D015534:Trans-Activators / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D016376:Oligonucleotides, Antisense; C508960:TGFB1 protein, human; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D051176:beta Catenin