title: [Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats].
abstract: OBJECTIVE
  To investigate the influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats, so as to provide a theoretical basis for the clinical use of hyperoxic fluid.
  
  
  METHODS
  Sprague-Dawley (SD) rats with 35% TBSA full-thickness scald were randomly divided into scald control (S, n = 6,with intraperitoneal fluid infusion after scalding), hyperoxic (H, n = 6, with hyperoxic fluid infusion after scalding) groups. Six rats without scald injury served as normal group. The proteins in the intestinal mucosa were separated with the two-dimensional electrophoresis (2-DE), and were analyzed with ImageMaster 2D Elite. The influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats was studied with bio-spectrum, protein bank and document analysis.
  
  
  RESULTS
  (1) Among the 34 down-regulated protein spots in S group, 9 of them definitely exhibited up-regulation compared with those in H group. (2) The expression of mitochondrial aconitase, alpha-propionyl-CoA carboxylase, short chain of hydroxyacyl-Coenzyme A dehydrogenase, transcription factor EB (estradiol benzoate), triosephosphate isomerase 1, T cell receptor V delta 6, and dynein-like protein-5 in H group were significantly up-regulated.
  
  
  CONCLUSIONS
  The hyperoxic fluid could up-regulate the down-regulated proteins in rat intestinal mucosa at early postburn stage, so that the barrier function of intestinal mucosa of rats with severe burns could be partially recovered.
authors.affiliation: General Surgery Department, The Sixth Municipal Hospital of Beijing, Beijing 100007, P.R. China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Sun
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Wang
authors.forename: Qin-Xue
authors.initials: QX
authors.lastname: Ding
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Guo-Cheng
authors.initials: GC
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011506:Proteins; D010100:Oxygen