title: Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.
abstract: Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.
authors.affiliation: Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Digman
authors.forename: Parijat
authors.initials: P
authors.lastname: Sengupta
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wiseman
authors.forename: Claire M
authors.initials: CM
authors.lastname: Brown
authors.forename: Alan R
authors.initials: AR
authors.lastname: Horwitz
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D000465:Algorithms; D000818:Animals; D001703:Biophysics / Q000379:methods; D002460:Cell Line; D006224:Cricetinae; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015233:Models, Statistical; D016011:Normal Distribution; D017785:Photons; D013997:Time Factors
chemical_list: D049452:Green Fluorescent Proteins