title: Phospholipid polymer surfaces reduce bacteria and leukocyte adhesion under dynamic flow conditions.
abstract: Persistence of infection can occur when the host immune response is compromised because of the presence of a foreign implant. Surface modification of biomaterials with phospholipid polymers may enhance biocompatibility and reduce incidence of infection by impeding bacterial and leukocyte adhesion. A rotating disk model, which generates shear stress from 0 to 18 dynes/cm(2), was used to characterize adhesion of neutrophils, monocytes, and bacteria in phosphate-buffered saline (PBS) or 25% human serum on polyethylene terephthalate surfaces coated with a phospholipid polymer, poly[omega-methacryloyloxyalkyl phosphorylcholine (MAPC)-co-n-butyl methacrylate (BMA)]. The material designated PMB30 contains a methylene chain length, (CH(2))(n), of n = 2, whereas PMHB30 contains a chain length of n = 6. In PBS, bacterial adhesion was shear stress dependent with the lowest bacterial density observed on PMB30. However, the presence of serum proteins eliminated shear stress and surface chemistry effects in addition to bacterial adhesion reduced to <10% of adhesion in PBS. Trends for leukocyte adhesion in serum demonstrated shear dependence with PMB30 exhibiting the lowest cell density throughout the range of shear stresses. In conclusion, modification of the polyethylene terephthalate surfaces with phospholipid polymers resulted in reduced bacterial and leukocyte adhesion. Furthermore, shortening the methylene chain length of the MAPC copolymer most effectively reduced adhesion.
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: Jasmine D
authors.initials: JD
authors.lastname: Patel
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.forename: James M
authors.initials: JM
authors.lastname: Anderson
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000502:physiology* / Q000648:ultrastructure; D008422:Materials Testing; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013210:Staphylococcus / Q000502:physiology / Q000648:ultrastructure; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D010743:Phospholipids; D011108:Polymers