Effect of a booster dose of serogroup B meningococcal vaccine on antibody response to Neisseria meningitidis in mice vaccinated with different immunization schedules.
The generation and maintenance of memory antibody response by different primary immunization schedules with the Cuban-produced outer membrane protein based vaccine was investigated in a murine model. We analyzed the duration of the antibody response (IgG-ELISA and bactericidal titer) and the effect of a booster dose on the antibody response. The IgG avidity index was determined in an attempt to find a marker for memory development. This study also included an analysis of IgG subclasses induced by primary and booster immunization. The specificity of bactericidal antibodies was investigated using local strains of the same serotype/serosubtype (4,7:P1.19,15) as the vaccine strain and mutant strains lacking major outer membrane proteins. A significant recall response was induced by a booster dose given 7 months after a primary series of 2, 3 or 4 doses of vaccine. The primary antibody response showed a positive dose-effect. In contrast, a negative dose-effect was found on the booster bactericidal antibody response. There was a significant increase in IgG1 levels after the fourth and booster doses. Three doses of vaccine were required to induce a significant increase in IgG avidity. Two injections of vaccine induced a significant antibody response to PorA protein, while 4 injections induced a larger range of specificities.
Gioia
Carolina A C
CA
Fundação Universidade Federal de Rio Grande, Hospital Universitário, Departamento de Patologia/Microbiologia e Imunologia, Rua Osório S/N. CEP: 96200400. Rio Grande, RS, Brasil.
de Sousa
Adriana B
AB
Cruz
Simone C
SC
Junior
Feliciano C S
FC
Andrade
Arnaldo F B
AF
Sassi
Raul M
RM
Frasch
Carl E
CE
Milagres
Lucimar G
LG
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000915:Antibody Affinity; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001770:Blood Bactericidal Activity; D005260:Female; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000145:classification; D007156:Immunologic Memory; D007700:Kinetics; D022401:Meningococcal Vaccines / Q000008:administration & dosage*; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines