title: Interstitial cells of Cajal in pancreas.
abstract: We show here (presumably for the first time) a special type of cell in the human and rat exocrine pancreas. These cells have phenotypic characteristics of the enteric interstitial cells of Cajal (ICC). To identify pancreatic interstitial cells of Cajal (pICC) we used routine light microscopy, non-conventional light microscopy (less than 1 mum semi-thin sections of Epon-embedded specimens cut by ultramicrotomy and stained with Toluidine blue), transmission electron microscopy (TEM), and immunocytochemistry. The results showed that pICC can be recognized easily by light microscopy, particularly on semi-thin sections, as well as by TEM. Two-dimensional reconstructions from serial photos suggest a network-like spatial distribution of pICC. pICC represent 3.3+/-0.5% of all pancreatic cells, and seem to establish close spatial relationships with: capillaries (43%), acini (40%), stellate cells (14%), nerve fibres (3%). Most of pICC (88%) have 2 or 3 long processes (tens of mum) emerging from the cell body. TEM data show that pICC meet the criteria for positive diagnosis as ICC (e.g. numerous mitochondria, 8.7+/-0.8% of cytoplasm). Immunocytochemistry revealed that pICC are CD117/c-kit and CD34 positive. We found pICC positive (40-50%) for smooth muscle alpha-actin or S-100, and, occasionally, for CD68, NK1 neurokinin receptor and vimentin. The reactions for desmin and chromogranin A were negative in pICC. At present, only hypotheses and speculations can be formulated on the possible role of the pICC (e.g., juxtacrine and/or paracrine roles). In conclusion, the quite-established dogma: "ICC only in cavitary organs" is overpassed.
authors.affiliation: Department of Cellular and Molecular Medicine, "Carol Davila" University of Medicine and Pharmacy, P.O. Box 35-29, Bucharest 35, Romania. LMP@univermed-cdgm.ro
authors.forename: L M
authors.initials: LM
authors.lastname: Popescu
authors.forename: M E
authors.initials: ME
authors.lastname: Hinescu
authors.forename: N
authors.initials: N
authors.lastname: Ionescu
authors.forename: Sanda M
authors.initials: SM
authors.lastname: Ciontea
authors.forename: D
authors.initials: D
authors.lastname: Cretoiu
authors.forename: Carmen
authors.initials: C
authors.lastname: Ardelean
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D048429:Cell Size; D020541:Coiled Bodies / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008751:Methylene Blue / Q000378:metabolism; D008954:Models, Biological; D010179:Pancreas / Q000166:cytology* / Q000648:ultrastructure; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000378:metabolism; D013194:Staining and Labeling; D014048:Tolonium Chloride / Q000378:metabolism
chemical_list: D000199:Actins; D015703:Antigens, CD; D009418:S100 Proteins; D014048:Tolonium Chloride; D019009:Proto-Oncogene Proteins c-kit; D008751:Methylene Blue