Interleukin-8 can stimulate progesterone secretion from a human trophoblast cell line, BeWo.
Precise paracrine cross-talk between the embryo and the endometrium is essential for the establishment of a successful pregnancy. Previous studies have demonstrated that the expression of interleukin-8 (IL-8) in the endometrium is enhanced during the late-secretory phase and early pregnancy. Furthermore, IL-8 receptor (IL-8R) expression has been detected in trophoblast cells of the developing embryo. To clarify the roles of IL-8 in the endometrium-embryo interactions, the effects of IL-8 on hormone secretion by trophoblast cells were studied using the BeWo trophoblast cell line that retains hormone-secreting properties of normal trophoblast cells. Using reverse transcription-polymerase chain reaction, we found that IL-8R messenger ribonucleic acid (mRNA) was expressed in BeWo cells. The levels of IL-8R mRNA and protein expression in BeWo cells were similar to those in primary first-trimester trophoblast cells. Progesterone (P4) secretion of BeWo cells was comparable with that of first-trimester trophoblast cells but higher than that of third-trimester trophoblast cells. Treatment of BeWo cells with recombinant human IL-8 (rhIL-8) had no effect on cell proliferation, as determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Interestingly, secretion of P4, but not human chorionic gonadotropin, from cultured BeWo cells was significantly enhanced when the cells were incubated with rhIL-8. Our results demonstrate that IL-8 may play an important role in the endometrium-embryo interactions by stimulating trophoblast secretion of P4 for maintenance of a successful pregnancy.
Tsui
Kuan-Hao
KH
Department of Obstetrics and Gynecology, Veterans General Hospital, 201 Section 2, Shih-Pai Road, Taipei, Taiwan 112, Republic of China,
Chen
Ling-Yun
LY
Shieh
Miawh-Lirng
ML
Chang
Sheng-Ping
SP
Yuan
Chiou-Chung
CC
Li
Hsin-Yang
HY
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017931:DNA Primers; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011863:Radioimmunoassay; D023063:Receptors, Interleukin-8B / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D014327:Trophoblasts / Q000378:metabolism*
D017931:DNA Primers; D016209:Interleukin-8; D012333:RNA, Messenger; D023063:Receptors, Interleukin-8B; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D011374:Progesterone; C022616:thiazolyl blue