Molecularly sensitive optical coherence tomography.
Molecular contrast in optical coherence tomography (OCT) is demonstrated by use of coherent anti-Stokes Raman scattering (CARS) for molecular sensitivity. Femtosecond laser pulses are focused into a sample by use of a low-numerical-aperture lens to generate CARS photons, and the backreflected CARS signal is interferometrically measured. With the chemical selectivity provided by CARS and the advanced imaging capabilities of OCT, this technique may be useful for molecular contrast imaging in biological tissues. CARS can be generated and interferometrically measured over at least 600 microm of the depth of field of a low-numerical-aperture objective.
Bredfeldt
Jeremy S
JS
Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.
Vinegoni
Claudio
C
Marks
Daniel L
DL
Boppart
Stephen A
SA
D000818:Animals; D002417:Cattle; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods*; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D009124:Muscle Proteins / Q000378:metabolism / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
D009124:Muscle Proteins