Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity.
Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.
Donohue
Maura J
MJ
Department of Chemistry, American University, Washington, DC 20016, USA. Donohue.maura@epa.gov
Satterfield
Mary B
MB
Dalluge
Joseph J
JJ
Welch
Michael J
MJ
Girard
James E
JE
Bunk
David M
DM
D002021:Buffers; D003959:Diamines; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D017430:Prostate-Specific Antigen / Q000235:genetics*; D020033:Protein Isoforms / Q000302:isolation & purification*; D015203:Reproducibility of Results
D002021:Buffers; D003959:Diamines; D020033:Protein Isoforms; C009475:trimethylenediamine; D017430:Prostate-Specific Antigen