title: Digital radiographic quantification of myocardial blood flow around a transmyocardial laser channel in rabbit hearts.
abstract: BACKGROUND
  A mechanism underlying the benefits of transmyocardial laser revascularization (TMLR) has been presumed to be improvement in perfusion. We evaluated myocardial blood flow around a laser channel using digital radiography combined with a 3H-labeled desmethylimipramine ([3H]DMI) deposition.
  
  
  RESULTS
  A laser channel was created in the left ventricular wall using a YAG-laser in 6 non-ischemic rabbit hearts. After 8 weeks, [3H]DMI(1.11 MBq) was injected into the left atrium and the TMLR-treated myocardium was sectioned. Another 6 hearts were examined as controls. We measured [3H]DMI density in arbitrary units with digital radiography in the channel remnant, the surrounding area and a remote area. Flow distribution was quantified by the coefficient of variation of flows (CV). The surrounding area had the highest density (p < 0.001) and the lowest CV (p < 0.001), and had higher density (p < 0.001) and lower CV (p < 0.001) than the controls. There was no transmural difference in the density in all domains. The CV increased with depth in the remote area, as well as in controls (p < 0.001), but there was no transmural difference in the surrounding area.
  
  
  CONCLUSIONS
  The TMLR increases myocardial blood flow and decreases flow heterogeneity in the surrounding area. The disappearance of transmural difference in flow heterogeneity might indicate the remodeling of microcirculation to improve regional oxygen delivery.
authors.affiliation: Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
authors.forename: Osami
authors.initials: O
authors.lastname: Honjo
authors.forename: Kozo
authors.initials: K
authors.lastname: Ishino
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Shu
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Asai
authors.forename: Takushi
authors.initials: T
authors.lastname: Kohmoto
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kajiya
authors.forename: Shunji
authors.initials: S
authors.lastname: Sano
mesh_terms: D000818:Animals; D003326:Coronary Circulation; D003891:Desipramine; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000601:surgery*; D053685:Laser Therapy; D023421:Models, Animal; D009204:Myocardial Revascularization / Q000379:methods*; D011817:Rabbits; D011856:Radiographic Image Enhancement; D012039:Regional Blood Flow; D014316:Tritium
chemical_list: D014316:Tritium; D003891:Desipramine