Expanding screening space through the use of alternative reservoirs in vapor-diffusion experiments.
Setting up vapor-diffusion crystallization experiments against four different reservoir solutions showed that the reservoir solution may have a profound effect on the outcome of a crystallization experiment. This suggests that a facile way to increase crystallization space through screening is not to add more crystallization conditions to the process, but to set up the same conditions over different reservoirs.
Newman
Janet
J
In Stilla Consulting, 736 Arden Drive, Encinitas, CA 92024, USA. xtaljanet@hotmail.com
D000645:Ammonium Sulfate; D003460:Crystallization / Q000295:instrumentation* / Q000379:methods*; D004058:Diffusion; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012965:Sodium Chloride
D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D009113:Muramidase; D000645:Ammonium Sulfate