title: Demonstration and operative influence of low prime volume closed pump.
abstract: Various improvements have been made in cardiopulmonary bypass (CPB) in the past few decades. We designed a new type of CPB to reduce the secretion of systemic inflammatory markers. We used a low prime volume pump (LPVP), completely closed CPB circuit and examined coagulant factors and inflammatory cytokines. In this study, we demonstrate the efficacy of LPVP using molecular biological data. Fourteen patients were randomized prospectively into two groups: Group L patients underwent LPVP (n = 8) and Group N patients underwent normal prime volume CPB (n = 6). We measured thrombin-antithrombin III complex (TAT), complement factor (C3a), and interleukin (IL)-10 levels at four time points. TAT (66.1 +/- 15.1 ng.mL(-1)), C3a (1895 +/- 282 ng.mL(-1)) and IL-10 (486 +/- 114 pg.mL(-1)) levels in Group N were significantly higher than in Group L (TAT, 19.5 +/- 4.4 ng.mL(-1); IL-10, 105 +/- 24.6 pg.mL(-1); C3a, 1349 +/- 369 ng.mL(-1)) immediately following CPB. LPVP demonstrated a lower systemic inflammatory response compared to normal prime volume CPB, as assessed using a molecular biological approach.
authors.affiliation: Department of Cardiovascular Surgery, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. shinge@net.nagasaki-u.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takai
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Eishi
authors.forename: Shiro
authors.initials: S
authors.lastname: Yamachika
authors.forename: Shiro
authors.initials: S
authors.lastname: Hazama
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Ariyoshi
authors.forename: Katsuo
authors.initials: K
authors.lastname: Nishi
mesh_terms: D000368:Aged; D000990:Antithrombin III; D002315:Cardiopulmonary Bypass / Q000379:methods*; D015926:Complement C3a / Q000378:metabolism; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000097:blood / Q000601:surgery*; D006400:Hematocrit; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000097:blood; D011446:Prospective Studies; D016896:Treatment Outcome
chemical_list: C046193:antithrombin III-protease complex; D016753:Interleukin-10; D015926:Complement C3a; D000990:Antithrombin III; D010447:Peptide Hydrolases