Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach.
BACKGROUND
Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.


RESULTS
A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.


CONCLUSIONS
Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.
Harney
D F
DF
Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, 123 St, Stephens Green, Dublin 2, Ireland. dharney@rcsi.ie
Butler
R K
RK
Edwards
R J
RJ
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D002645:Chickens; D019295:Computational Biology / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D019000:Phosphotyrosine; D018995:Myosin Heavy Chains