Generation of recombinant norovirus-like particles (VLP) in the human endothelial kidney cell line 293T.
Noroviruses (NVs) are the major cause of non-bacterial outbreaks of gastroenteritis. Here we report a new alternative system to generate recombinant NV virus-like particles (rNV-VLP) in a human endothelial kidney cell line (HEK). Transfecting HEK-293T cells with an expression vector coding for the ORF-2 gene lead to the expression of the viral structural protein VP1 which spontaneously assembled into virus-like particles (VLP), as shown by electron microscopy. The transfected cells did not show a cytopathic effect and released rNV-VLP into the culture medium. The HEK-293T cell derived particles were morphologically indistinguishable to the rNV-VLP produced from baculovirus and the Venezuelan equine encephalitis virus (VEE)-replicon. The produced particles were stable for at least 2.5 months at 4 degrees C.
Taube
S
S
Robert Koch-Institut, Berlin, Germany.
Kurth
A
A
Schreier
E
E
D002460:Cell Line; D042783:Endothelial Cells / Q000821:virology*; D006801:Humans; D007668:Kidney / Q000166:cytology*; D029322:Norovirus / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
D011994:Recombinant Proteins