Uncovering hidden in vivo resonances using editing based on localized TOCSY.
A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.
Marjanska
Malgorzata
M
Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota, 2021 6th Street SE, Minneapolis, MN 55455, USA. gosia@cmrr.umn.edu
Henry
Pierre-Gilles
PG
Bolan
Patrick J
PJ
Vaughan
Brooks
B
Seaquist
Elizabeth R
ER
Gruetter
Rolf
R
Uğurbil
Kâmil
K
Garwood
Michael
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D012815:Signal Processing, Computer-Assisted
D019344:Lactic Acid; D005947:Glucose