Effect of a fermented ginseng extract, BST204, on the expression of cyclooxygenase-2 in murine macrophages.
This paper investigates how BST204, a fermented ginseng extract, affects the expression and mechanism of cyclooxygenase-2 (COX-2). BST204 was prepared by incubating crude ginseng extract with ginsenoside-beta-glucosidase. Unexpectedly, BST204 had no effect on the level of COX-2 protein in unstimulated RAW 264.7 cells, and it suppressed the level of COX-2 protein and PGE(2) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. It did not show any suppressive effect, though, on the COX-2 mRNA level. To investigate the suppressive mechanism of COX-2 protein, the activating phosphorylation of p70 S6 kinase and 4E-BP1, which are important for translation, were measured. The phosphorylation of p70 S6 kinase, not 4E-BP1, was increased by LPS in a time-dependent manner, and was inhibited by BST204 in a dose-dependent manner. The expression of COX-2 protein, however, was partially suppressed by rapamycin, an upstream inhibitor of p70 S6 kinase. Therefore, this paper suggests that the suppression of COX-2 protein by BST204 was partially correlated with the inhibition of p70 S6 kinase activation.
Seo
Jin Young
JY
Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, South Korea.
Lee
Jun Ho
JH
Kim
Nam Wook
NW
Her
Erk
E
Chang
Sung Ho
SH
Ko
Na Young
NY
Yoo
Young Hyo
YH
Kim
Jie Wan
JW
Seo
Dong Wan
DW
Han
Jeung Whan
JW
Kim
Young Mi
YM
Choi
Wahn Soo
WS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D051546:Cyclooxygenase 2; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D005285:Fermentation; D015870:Gene Expression / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology*; D051379:Mice; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology
D018076:DNA, Complementary; D036145:Ginsenosides; D010936:Plant Extracts; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D020123:Sirolimus