title: Genotyping of Norovirus strains detected in outbreaks between April 2002 and March 2003 in Osaka City, Japan.
abstract: Noroviruses (NVs) are the major cause of food- and waterborne nonbacterial gastroenteritis in Japan. Between April 2002 and March 2003, a total of 111 fecal specimens from 40 outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan were subject to NV detection. Seventy-two samples (64.9%) from 31 outbreaks (77.5%) were NV positive by a real time reverse transcription (RT)-PCR assay. To further determine the genotype of individual NV strains, we sequenced the capsid N-terminal/shell (N/S) domain of some representative strains from each outbreak. The 51 NV strains detected in this study were segregated into 15 genotypes (6 in genogroup I and 9 in genogroup II), and GII/5 genotype NV was a dominant outbreak genotype.
authors.affiliation: Department of Virology, Osaka City University Medical School, Osaka, Japan. yoshi3486@med.osaka-cu.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seto
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Iritani
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kubo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaida
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Murakami
authors.forename: Kosuke
authors.initials: K
authors.lastname: Haruki
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishio
authors.forename: Minoru
authors.initials: M
authors.lastname: Ayata
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ogura
mesh_terms: D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins