title: Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery.
abstract: The emergence of a relatively new technique resulting from a combination of frontal affinity chromatography coupled with MS detection (FAC-MS) has extended the capabilities of MS in drug discovery and development. Its application in a broad range of biological systems, together with its label-free operation, relatively high throughput, ability to rank ligands and determine Kd, makes FAC-MS a universal tool enabling convenient and efficient screening in the identification of new potential drug leads. Here we will highlight FAC-MS screening studies and discuss where it can be applied in evaluating multiple protein-binding sites, protein-protein interactions and inactive proteins, and also in determining selectivity.
authors.affiliation: Protana, Lead Discovery and Optimization Division, 251 Attwell Drive, Toronto, ON M9W 7H4, Canada. jslon@protana.com
authors.forename: Jacek J
authors.initials: JJ
authors.lastname: Slon-Usakiewicz
authors.forename: William
authors.initials: W
authors.lastname: Ng
authors.forename: Jin-Rui
authors.initials: JR
authors.lastname: Dai
authors.forename: Andrew
authors.initials: A
authors.lastname: Pasternak
authors.forename: Peter R
authors.initials: PR
authors.lastname: Redden
mesh_terms: D001665:Binding Sites; D002846:Chromatography, Affinity; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008024:Ligands; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D011506:Proteins