Influence of insulin immobilization to thermoresponsive culture surfaces on cell proliferation and thermally induced cell detachment.
Temperature-responsive culture dishes immobilized with insulin have been fabricated and studied to shorten cell culture periods by facilitating more rapid cell proliferation. Cells are recovered as contiguous cell sheets simply by temperature changes. Functionalized culture dishes were prepared by previously reported electron beam grafting copolymerization of N-isopropylacrylamide (IPAAm) with its carboxylate-derivatized analog, 2-carboxyisopropylacrylamide (CIPAAm), having similar molecular structure to IPAAm but with carboxylate side chains to tissue culture polystyrene dishes. Insulin was then immobilized onto culture dishes through standard amide bond formation with CIPAAm carboxylate groups. Adhesion and proliferation of bovine carotid artery endothelial cells (ECs) were examined on these insulin-immobilized dishes. Insulin immobilization was shown to promote cell proliferation in serum-supplemented medium. Increasing the grafted CIPAAm content on the tissue culture surfaces reduces cell adhesion and proliferation, even though these surfaces contained increased amounts of immobilized insulin. This result implies that a discrete balance exists between the amount of CIPAAm-free carboxylate groups and immobilized insulin for optimum cell proliferative stimulation. Cells grown on the insulin-immobilized surfaces can be recovered as contiguous cell monolayers simply by lowering culture temperature, without need for exogenous enzyme or calcium chelator additions. In conclusion, insulin-modified thermoresponsive culture dishes may prove useful for advanced cell culture and tissue engineering applications since they facilitate cell proliferation, and cultured cells can be recovered as viable contiguous monolayers by merely reducing culture temperature.
Hatakeyama
Hideyuki
H
Center of Excellence Program for the 21st Century, Tokyo Women's Medical University, and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo 162-8666, Japan.
Kikuchi
Akihiko
A
Yamato
Masayuki
M
Okano
Teruo
T
D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects*; D003080:Cold Temperature; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*
D000178:Acrylamides; D016895:Culture Media, Serum-Free; D005353:Fibronectins; D007328:Insulin; C404844:poly(IPAAm-co-CIPAAm)