title: Isolation and identification of bacteria from spent nuclear fuel pools.
abstract: The aim of the present research was to isolate and identify bacteria from spent nuclear fuel pools of a Spanish nuclear power plant. Water samples were collected and inoculated onto different culture media to isolate the highest number of species. 16S rDNA fragments from colonies growing on solid media were amplified and analysed by denaturing gradient gel electrophoresis. Sequencing revealed the presence of 21 different bacteria belonging to several phylogenetic groups (alpha, beta, and gamma-Proteobacteria, Actinomycetales, Flavobacterium, and the Bacillus/Staphylococcus group). The isolation of these microorganisms in this particular environment (oligotrophic and radioactive) is highly interesting because of the possibility of their being used for the bioremediation of radionuclide-contaminated waters.
authors.affiliation: Departamento de Ingeniería y Ciencia de los Materiales, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006, Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Chicote
authors.forename: Ana M
authors.initials: AM
authors.lastname: García
authors.forename: Diego A
authors.initials: DA
authors.lastname: Moreno
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Sarró
authors.forename: Petra I
authors.initials: PI
authors.lastname: Lorenzo
authors.forename: Felipe
authors.initials: F
authors.lastname: Montero
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D004586:Electrophoresis / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D011210:Power Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D014871:Water Microbiology; D014878:Water Pollution, Radioactive
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S