'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins.
Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.
Brown
D D
DD
School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
Collins
F M
FM
School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
Duprex
W P
WP
Centre for Cancer Research and Cell Biology, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.|School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
Baron
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MD
The Institute for Animal Health, Pirbright, Surrey, UK.
Barrett
T
T
The Institute for Animal Health, Pirbright, Surrey, UK.
Rima
B K
BK
Centre for Cancer Research and Cell Biology, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.|School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004217:Distemper Virus, Canine / Q000235:genetics / Q000502:physiology; D004285:Dogs; D017930:Genes, Reporter; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D008459:Measles virus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011995:Recombination, Genetic; D012302:Rinderpest virus / Q000235:genetics* / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D014764:Viral Proteins