A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins.
The organization of living cells is based on networks of interacting molecules. Systematic analysis of protein interactions of 3-aa loop extension (TALE) homeodomain proteins, fundamental regulators of plant meristem function and leaf development, revealed a highly connected, complex network. The network includes nine members of Arabidopsis thaliana ovate family proteins (AtOFPs), a plant-specific protein family, indicating a close functional connection to TALE homeodomain proteins. Evidence is provided that AtOFP1 is an essential pleiotropic developmental regulator. AtOFP1 and AtOFP5 are shown to associate with the cytoskeleton and to regulate subcellular localization of TALE homeodomain proteins, suggesting a previously unrecognized control mechanism in plant development.
Hackbusch
Jana
J
Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
Richter
Klaus
K
Müller
Judith
J
Salamini
Francesco
F
Uhrig
Joachim F
JF
D016960:Agrobacterium tumefaciens; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005453:Fluorescence; D005822:Genetic Vectors; D018398:Homeodomain Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000235:genetics / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae; D014162:Transfection; D020798:Two-Hybrid System Techniques
D017931:DNA Primers; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D010940:Plant Proteins