title: Saccharomyces cerevisiaeTSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex.
abstract: Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11(ts) mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.
authors.affiliation: Institute of Biochemistry, School of Life Sciences and Department of Biochemistry, School of Medicine, National Yang-Ming University, 155, Sec. 2, Li-Nong St., Shih-Pai, Taipei, 112, Taiwan.
authors.forename: Hsiang-Ling
authors.initials: HL
authors.lastname: Ho
authors.forename: Yu-Shih
authors.initials: YS
authors.lastname: Shiau
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D001483:Base Sequence; D002110:Caffeine / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000235:genetics; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000235:genetics; D018628:Gene Dosage; D005804:Genes, Lethal; D016153:Genes, Suppressor; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000199:Actins; D002110:Caffeine; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D046912:Multiprotein Complexes; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D019869:Phosphatidylinositol 3-Kinases; D029701:Saccharomyces cerevisiae Proteins; C503706:Avo1 protein, S cerevisiae; C503707:Avo2 protein, S cerevisiae; C502930:Slm1 protein, S cerevisiae; C418219:TSC13 protein, S cerevisiae; C081135:TOR2 protein, S cerevisiae