The identification of foam-forming soluble proteins from wheat (Triticum aestivum) dough.
Proteomic methods have been used to identify foam-forming soluble proteins from dough that may play an important role in stabilising gas bubbles in dough, and hence influence the crumb structure of bread. Proteins from a soluble fraction of dough (dough liquor) or dough liquor foam have been separated by two-dimensional gel electrophoresis, and 42 identified using a combination of matrix-assisted laser desorption/ionization-time of flight and quadrupole-time of flight analyses. Major polypeptide components included beta-amylase, tritin and serpins, with members of the alpha-amylase/trypsin inhibitor family being particularly abundant. Neither prolamin seed storage proteins nor the surface-active protein puroindoline were found. Commonly used dough ingredients (NaCl, Na L-ascorbate) had only a minor effect on the 2-DE protein profiles of dough liquor, of which one of the more significant was the loss of 9 kDa nonspecific lipid transfer protein. Many proteins were lost in dough liquor foam, particularly tritin, whilst a number of alpha-amylase inhibitors were more dominant, suggesting that these are amongst the most strongly surface-active proteins in dough liquor. Such proteins may play a role determining the ability of the aqueous phase of doughs, as represented by dough liquor, to form an elastic interface lining the bubbles, and hence maintain their integrity during dough proving.
Salt
Louise J
LJ
Institute of Food Research, Norwich Research Park, Colney, Norwich, UK.
Robertson
James A
JA
Jenkins
John A
JA
Mulholland
Francis
F
Mills
E N Clare
EN
D001205:Ascorbic Acid; D001939:Bread; D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics; D012965:Sodium Chloride; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013501:Surface-Active Agents / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
D010940:Plant Proteins; D013501:Surface-Active Agents; D012965:Sodium Chloride; D001205:Ascorbic Acid