title: The detection of oxycodone in meconium specimens.
abstract: A procedure for the determination of oxycodone in meconium using direct ELISA microplate technology followed by electron impact gas chromatography-mass spectrometry (GC-MS) is described for the first time. The abuse of oxycodone (OxyContin) has been widely discussed in mainstream media, and it has been described as a cheap form of heroin. Oxycodone has been reported as having a high degree of abuse and potential complications in neonates from maternal drug use. Using a standard enzyme multiplied immunoassay screening technology, the cross-reactivity of oxycodone to the morphine antibody is only 5-6%. A positive screening value would require a high concentration of drug to be present, so a protocol for the detection of oxycodone in meconium using a direct ELISA microplate immunoassay followed by GC-MS was developed. The assay is now routinely used in our laboratory.
authors.affiliation: U.S. Drug Testing Laboratories, 1700 S. Mount Prospect Road, Des Plaines, Illinois 60018, USA.
authors.forename: Ngoc L
authors.initials: NL
authors.lastname: Le
authors.forename: Andrea
authors.initials: A
authors.lastname: Reiter
authors.forename: Kimberly
authors.initials: K
authors.lastname: Tomlinson
authors.forename: Joseph
authors.initials: J
authors.lastname: Jones
authors.forename: Christine
authors.initials: C
authors.lastname: Moore
mesh_terms: D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D009020:Morphine / Q000276:immunology; D009294:Narcotics / Q000032:analysis* / Q000276:immunology; D010098:Oxycodone / Q000032:analysis* / Q000276:immunology
chemical_list: D009294:Narcotics; D009020:Morphine; D010098:Oxycodone