Ischemic preconditioning improves rat kidney graft function after severe ischemia/reperfusion injury.
Ischemic preconditioning (IP) has been shown to ameliorate renal ischemia reperfusion injury. Using a rat kidney transplantation model we determined if IP improves graft function after prolonged cold storage.


METHODS
Syngeneic rat kidneys were divided into two groups. Prior to 42 hours of cold storage in UW and transplantation, one group (n = 10) received IP (15 minutes of warm ischemia/10 minutes of reperfusion), whereas another group (n = 10) received no treatment. Early graft function and 1-week recipient survival were assessed.


RESULTS
Recipient survival was not significantly different between groups [70% (IP) vs 40% (non-IP); P = .28]. IP treatment led to a quicker recovery of renal function. On PODs 3 and 6, serum creatinine levels in the IP group were significantly lower compared with the untreated group. In conclusion, one cycle of IP (15/10) accelerates recovery of renal graft function after severe ischemia reperfusion injury. This simple treatment modality may improve outcomes of renal transplants with prolonged cold storage.
Fuller
T F
TF
Department of Urology, Charité Hospital, Humboldt University, Berlin, Germany. flofu@yahoo.com
Freise
C E
CE
Feng
S
S
Niemann
C U
CU
D000818:Animals; D003404:Creatinine / Q000097:blood; D006085:Graft Survival; D019194:Ischemic Preconditioning / Q000379:methods*; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000517:prevention & control*; D014185:Transplantation, Isogeneic / Q000502:physiology
D003404:Creatinine