Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA.
Viral infection in the airway provokes various immune responses, including Th1 and Th2 responses, which are partly initiated by double-stranded RNA (dsRNA), a viral product for its replication. B7-H1 (PD-L1) and B7-DC (PD-L2) are B7-family molecules that bind to programmed death-1 (PD-1) on lymphocytes and are implicated in peripheral tolerance. We investigated the effect of dsRNA on the expression of B7-H1 and B7-DC on airway epithelial cell lines. B7-H1 and B7-DC were constitutively expressed on the cells, and their expression was profoundly upregulated by stimulation with an analog of viral dsRNA, polyinosinic-polycytidylic acid. B7-H1 and B7-DC were also upregulated by stimulation with IFN-gamma, IL-13, and the supernatant from T cell clones. A relatively high concentration of dexamethasone (1 microM) was required to suppress the upregulation of B7-H1 or B7-DC. These results suggest that epithelial B7-H1 and B7-DC play a role in virus-associated immune responses in the airways.
Tsuda
Miyuki
M
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Matsumoto
Koichiro
K
Inoue
Hiromasa
H
Matsumura
Mikiko
M
Nakano
Takako
T
Mori
Akio
A
Azuma
Miyuki
M
Nakanishi
Yoichi
Y
D015703:Antigens, CD; D018122:B7-1 Antigen / Q000378:metabolism*; D060890:B7-H1 Antigen; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002461:Cell Line, Transformed; D002999:Clone Cells; D006801:Humans; D016899:Interferon-beta / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D061027:Programmed Cell Death 1 Ligand 2 Protein; D012330:RNA, Double-Stranded / Q000502:physiology*
D015703:Antigens, CD; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D018793:Interleukin-13; D008562:Membrane Glycoproteins; C556977:PDCD1LG2 protein, human; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D012330:RNA, Double-Stranded; D016899:Interferon-beta; D007371:Interferon-gamma