Polymerase chain reaction for identification of herpes simplex virus (HSV-1), cytomegalovirus (CMV) and human herpes virus-type 6 (HHV-6) in oral swabs.
Considering that sensitive and specific methods to detect HSV-1, CMV and HHV-6 on oral mucosa have a great impact on oral diagnosis practice and research, together with the evidence that PCR is a rapid and reliable method, the purpose of the present study was to develop primer sets to detect HSV-1, CMV and HHV-6 in oral swabs by nested polymerase chain reaction (nested PCR). We developed a practical method for sample collection without tissue trauma, and the swabs were stored until used for DNA extraction. After the nested PCR a DNA fragment of 241 bp corresponding to HSV-1 was amplified. DNA fragments of 224 and 369 bp were amplified corresponding to CMV and HHV-6, respectively. DNA sequencing analysis confirmed the expected sequences of each virus. In conclusion, it was demonstrated that these new primer sets are able to identify HSV-1, CMV and HHV-6 in oral swab using nested PCR.
Victória
Jùnia Maria Netto
JM
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, CEP 31270 901 Belo Horizonte-MG, Brazil.
Guimarães
André Luiz Sena
AL
da Silva
Luciano Marques
LM
Kalapothakis
Evanguedes
E
Gomez
Ricardo Santiago
RS
D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D006561:Herpes Simplex / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D009061:Mouth Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019349:Roseolovirus Infections / Q000175:diagnosis; D017421:Sequence Analysis
D017931:DNA Primers; D004279:DNA, Viral