title: Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts.
abstract: In a quantitative comparative study, we elucidated the glucose metabolism in fourteen hemiascomycetous yeasts from the Genolevures project. The metabolic networks of these different species were first established by (13)C-labeling data and the inventory of the genomes. This information was subsequently used for metabolic-flux ratio analysis to quantify the intracellular carbon flux distributions in these yeast species. Firstly, we found that compartmentation of amino acid biosynthesis in most species was identical to that in Saccharomyces cerevisiae. Exceptions were the mitochondrial origin of aspartate biosynthesis in Yarrowia lipolytica and the cytosolic origin of alanine biosynthesis in S. kluyveri. Secondly, the control of flux through the TCA cycle was inversely correlated with the ethanol production rate, with S. cerevisiae being the yeast with the highest ethanol production capacity. The classification between respiratory and respiro-fermentative metabolism, however, was not qualitatively exclusive but quantitatively gradual. Thirdly, the flux through the pentose phosphate (PP) pathway was correlated to the yield of biomass, suggesting a balanced production and consumption of NADPH. Generally, this implies the lack of active transhydrogenase-like activities in hemiascomycetous yeasts under the tested growth condition, with Pichia angusta as the sole exception. In the latter case, about 40% of the NADPH was produced in the PP pathway in excess of the requirements for biomass production, which strongly suggests the operation of a yet unidentified mechanism for NADPH reoxidation in this species. In most yeasts, the PP pathway activity appears to be driven exclusively by the demand for NADPH.
authors.affiliation: Institute of Biotechnology, Building HPT E58, ETH Zürich, 8093 Zürich, Switzerland. blank@biotech.biol.ethz.ch
authors.forename: Lars M
authors.initials: LM
authors.lastname: Blank
authors.forename: Frank
authors.initials: F
authors.lastname: Lehmbeck
authors.forename: Uwe
authors.initials: U
authors.lastname: Sauer
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D002952:Citric Acid Cycle; D015966:Gene Expression Regulation, Fungal; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway
chemical_list: D002247:Carbon Isotopes; D009249:NADP