Determination of deracoxib in feline plasma samples using high performance liquid chromatography.
A new HPLC procedure for the determination of deracoxib, a selective cyclooxygenase-2 inhibitor, has been developed and validated. Following a liquid-liquid extraction using isopropyl alcohol and chloroform, samples were separated by isocratic reversed-phase HPLC on an Atlantis C18 column and quantified using UV detection at 252 nm. The mobile phase was a mixture of 10 mM potassium phosphate (pH 4.5) and acetonitrile, with a flow-rate of 1.0 ml/min. The procedure produced a linear curve over the concentration range 10-1500 ng/ml. The development of the assay allowed the determination of pharmacokinetic parameters after oral administration of deracoxib in cats and would be suitable for other pharmacokinetic studies.
Cox
Sherry K
SK
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37966, USA. scox6@utk.edu
Roark
Jacob
J
Gassel
Adam
A
Tobias
Karen
K
D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000097:blood*; D000096926:Benzenesulfonamides
D016861:Cyclooxygenase Inhibitors; D013449:Sulfonamides; C471996:deracoxib; D000096926:Benzenesulfonamides