Radioprotective effect of sesamol on gamma-radiation induced DNA damage, lipid peroxidation and antioxidants levels in cultured human lymphocytes.
Sesamol pretreated (1, 5 and 10 microg/ml) lymphocytes were exposed to different doses of gamma-radiation, i.e., 1, 2 and 4 Gray (Gy) and the cellular changes were estimated by using cytokinesis blocked micronucleus assay (MN), dicentric aberration (DC), thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Radiation significantly increased MN, DC frequencies, TBARS levels and decreased GSH and antioxidant enzyme levels in a dose dependent manner. The highest damage to lymphocytes was observed at 4 Gy irradiation. On the other hand, sesamol pretreatment significantly decreased MN, DC frequencies, TBARS levels and increased GSH levels and SOD, CAT and GPx activities in a concentration dependent manner. At 1 Gy irradiation all concentrations of sesamol (1, 5 and 10 microg/ml) significantly protects the lymphocytes from radiation damage. At 2 Gy irradiation 5 and 10 microg/ml of sesamol shows significant radioprotection. Since the highest damage was observed at 4 Gy irradiation both 1 and 5 microg/ml of sesamol pretreatment were not sufficient to protect the lymphocytes from radiation damage but 10 microg/ml of sesamol significantly (p<0.05) protects the lymphocytes from radiation effect. Thus, sesamol pretreatment gives significant protection to cultured human lymphocytes against gamma-radiation induced cellular damage. The possible mechanism involved in the radioprotective influence of sesamol is discussed.
Prasad
N Rajendra
NR
Department of Biochemistry, Annamalai University, Annamalainagar 608002, Tamilnadu, India. nrprasadnr@rediffmail.com
Menon
Venugopal P
VP
Vasudev
V
V
Pugalendi
K V
KV
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D052117:Benzodioxoles; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004249:DNA Damage; D005720:Gamma Rays; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D048629:Micronuclei, Chromosome-Defective; D010636:Phenols / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D052117:Benzodioxoles; D010636:Phenols; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; C025583:sesamol; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione