Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major).
A cDNA encoding hepcidin was isolated from a library of cDNA from spleen of red sea bream (Chrysophrys major) by expressed sequence tag analysis. The expression of the hepcidin mRNA in various tissues was examined. Challenge of red sea bream with Escherichia coli DH5alpha elevated hepcidin mRNA levels in spleen, gill, liver, and intestine.
Chen
Song-Lin
SL
Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Nanjing Road 106, 266071 Qingdao, People's Republic of China. chensl@ysfri.ac.cn
Xu
Mei-Yu
MY
Ji
Xiang-Shan
XS
Yu
Guo-Cai
GC
Liu
Yang
Y
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D064451:Hepcidins; D008969:Molecular Sequence Data; D009928:Organ Specificity; D021541:Sea Bream / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D064451:Hepcidins