Solid films of blended poly(vinyl alcohol)/poly(vinyl pyrrolidone) for topical S-nitrosoglutathione and nitric oxide release.
Nitric oxide (NO) is responsible for biological actions in mammals, ranging from the control of arterial pressure to immunological responses. In this study, S-nitrosoglutathione (GSNO), a spontaneous NO donor, was incorporated in solid films of blended poly(vinyl alcohol) (PVA) and poly(vinyl pyrrolidone) (PVP) comprising a biomaterial with potential for the local delivery of NO. In dry conditions, the extinction of the absorption bands of GSNO was correlated with the increase of the absorption band of its dimmer, GS-SG, implying NO release through the homolytic cleavage of the S-N bond. Mass spectrometry was used to confirm and to monitor the release of free NO from solid PVA/PVP-GSNO films to the gas phase. Kinetic measurement based on the Griess reaction was used to show that solid PVA/PVP-GSNO films are also capable of releasing both NO and GSNO to aqueous solution trough diffusion. Storage experiments have shown that GSNO is highly stabilized in the dry PVA/PVP matrix. The results indicate that GSNO-containing PVA/PVP films may be used for delivering free NO and/or GSNO topically and controllably.
Seabra
Amedea Barozzi
AB
Instituto de Química, Universidade Estadual de Campinas, UNICAMP, CP 6154, CEP 13083-970 Campinas, SP Brazil.
da Rocha
Lilian Lúcia
LL
Eberlin
Marcos N
MN
de Oliveira
Marcelo Ganzarolli
MG
D000287:Administration, Topical; D004058:Diffusion; D007700:Kinetics; D013058:Mass Spectrometry; D020030:Nitric Oxide Donors / Q000008:administration & dosage* / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010592:Pharmaceutic Aids / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D026422:S-Nitrosoglutathione / Q000008:administration & dosage* / Q000494:pharmacology*; D012996:Solutions; D012997:Solvents; D014867:Water
D020030:Nitric Oxide Donors; D010592:Pharmaceutic Aids; D012996:Solutions; D012997:Solvents; D014867:Water; D026422:S-Nitrosoglutathione; D011142:Polyvinyl Alcohol; D011205:Povidone