Lysyl oxidase expression in bronchogenic carcinoma.
BACKGROUND
Lysyl oxidase catalyzes a key step in the cross-linking of collagen and elastin in the extracellular matrix. Recent studies have documented differential lysyl oxidase expression in the stromal reaction to colon, breast, prostate, and lung cancer. The present study was undertaken to test the hypothesis that lysyl oxidase mRNA and protein expression decrease with advancing tumor stage in patients with bronchogenic carcinoma.


METHODS
Tumor specimens were obtained from 17 patients undergoing resection for bronchogenic carcinoma. Real-time polymerase chain reaction was used to determine steady-state lysyl oxidase mRNA expression, and protein expression was qualitatively assessed by immunohistochemistry.


RESULTS
Real-time polymerase chain reaction studies documented a 3.4-fold graded decrease in lysyl oxidase mRNA levels as tumors progressed from stage I to IV. Similar qualitative changes in lysyl oxidase protein expression were demonstrated by immunohistochemistry.


CONCLUSIONS
These results support the hypothesis that variations in lysyl oxidase expression may correlate with the invasive and metastatic potential of bronchogenic carcinoma.
Woznick
Amy R
AR
Department of Surgery, William Beaumont Hospital Research Institute, 3811 W. Thirteen Mile Rd., Royal Oak, MI 48073-6769, USA.
Braddock
Amy L
AL
Dulai
Mohanpal
M
Seymour
Marilyn L
ML
Callahan
Rose E
RE
Welsh
Robert J
RJ
Chmielewski
Gary W
GW
Zelenock
Gerald B
GB
Shanley
Charles J
CJ
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D002283:Carcinoma, Bronchogenic / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008168:Lung / Q000201:enzymology / Q000473:pathology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D009367:Neoplasm Staging; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D008249:Protein-Lysine 6-Oxidase