Bran extracts from pigmented rice seeds inhibit tumor promotion in lymphoblastoid B cells by phorbol ester.
Using flow cytometry, we quantitatively evaluated anti-tumor-promoting activity of rice bran extracts by measuring inhibition of Epstein-Barr virus early-antigen activation (EBV-EA) induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). This assay measures anti-tumor-promoting activity and cytotoxicity of target substances using the same batch of cells. Using this short-term procedure, we have determined the anti-tumor-promoting activity of 70% ethanol-water extracts of bran (outer layer) from seeds of five pigmented rice cultivars: Jumlalocal-1, DZ 78, Elwee, LK1-3-6-12-1-1, and LK1A-2-12-1-1. The results show that, compared to an extract from the non-pigmented white cooking rice variety Chuchung, the extracts from the pigmented varieties strongly inhibited phorbol ester-induced tumor promotion in marmoset lymphoblastoid cells B95-8 in vitro. These findings may facilitate development and use of new health-promoting rice varieties.
Nam
S H
SH
Department of Biological Science, Ajou University, Suwon 443-749, Republic of Korea.
Choi
S P
SP
Kang
M Y
MY
Koh
H J
HJ
Kozukue
N
N
Friedman
Mendel
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000187:drug effects* / Q000821:virology; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002470:Cell Survival; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014775:Virus Activation / Q000187:drug effects
D016588:Anticarcinogenic Agents; D000956:Antigens, Viral; D002273:Carcinogens; C033305:Epstein-Barr virus early antigen; D010936:Plant Extracts; D013755:Tetradecanoylphorbol Acetate