Human mesenchymal stem cells are tolerized by mice and improve skin and spinal cord injuries.
BACKGROUND
We sought to use human mesenchymal stem cells (HMSC) for skin and spinal cord repair in mice.


METHODS
Human bone marrow obtained from a young healthy donor was used to separate and culture human mesenchymal stem cells (HMSC). Ten mice were included in each of four groups. A full-thickness skin defect was surgically performed on all mice in groups 1 and 2. A transverse complete medullar section was performed in groups 3 and 4. Groups 1 and 3 received HMSC IV infusion and local HMSC polymer implant. Groups 2 and 4 received only the IV HMSC infusion. Five control animals from each group went through the same lesions but they didn't receive treatment.


RESULTS
After local administration of HMSC into the fibrin polymer combined with the IV infusion of HMSC, there was no immune rejection; all skin defects healed without scar or retraction at a median time of 14 days. Sixty percent of the animals treated with IV infusion and polymer with HMSC simultaneously had improved neurological activities, while all control mice with spinal cord injury experiments died or perpetuated their paralysis with worsening muscular atrophy and increasing propensity to skin damage.


CONCLUSIONS
HMSC are not immunologically reactive and can trespass species defense barriers. Animals treated with these cells repaired injuries better than controls. In this way we propose that universal HMSC from donors can be cultured, expanded, and cryopreserved to be used in human organ or tissue regeneration.
Mansilla
E
E
National University of La Plata, La Plata, Argentina.
Marin
G H
GH
Sturla
F
F
Drago
H E
HE
Gil
M A
MA
Salas
E
E
Gardiner
M C
MC
Piccinelli
G
G
Bossi
S
S
Salas
E
E
Petrelli
L
L
Iorio
G
G
Ramos
C A
CA
Soratti
C
C
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D012867:Skin / Q000293:injuries*; D013119:Spinal Cord Injuries / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous