Whole-genome expression profiling of Xylella fastidiosa in response to growth on glucose.
Xylella fastidiosa is the etiologic agent of diseases in a wide range of economically important crops including citrus variegated chlorosis, a major threat to the Brazilian citrus industry. The genomes of several strains of this phytopathogen have been completely sequenced enabling large-scale functional studies. In this work we used whole-genome DNA microarrays to investigate the transcription profile of X. fastidiosa grown in defined media with different glucose concentrations. Our analysis revealed that while transcripts related to fastidian gum production were unaffected, colicin-V-like and fimbria precursors were induced in high glucose medium. Based on these results, we suggest a model for colicin-defense mechanism in X. fastidiosa.
Pashalidis
Stefano
S
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brasil.
Moreira
Leandro M
LM
Zaini
Paulo A
PA
Campanharo
João C
JC
Alves
Lúcia M C
LM
Ciapina
Luciane P
LP
Vêncio
Ricardo Z N
RZ
Lemos
Eliana G M
EG
Da Silva
Aline M
AM
Da Silva
Ana C R
AC
D000595:Amino Acid Sequence; D049109:Cell Proliferation; D003001:Cloning, Molecular; D003087:Colicins / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D015854:Up-Regulation; D044167:Xylella / Q000235:genetics*
D003087:Colicins; D018076:DNA, Complementary; D012333:RNA, Messenger; D005947:Glucose