Formic acid solubilization of marine biological tissues for multi-element determination by ETAAS and ICP-AES.
A simple, fast method is described for the determination of Ag, As, Cd, Cu, Cr, Fe, Ni, and Se in marine biological tissues by electrothermal atomic-absorption spectrometry (ETAAS) and Na, Ca, K, Mg, Fe, Cu, and Mn by inductively coupled plasma-atomic emission spectrometry (ICP-AES). Solubilization of the biological tissue was achieved by using formic acid with vortex mixing followed by heating to 50 degrees C in an ultrasonic bath. Once solubilized, the tissues were diluted to an appropriate volume with water for analysis. Aliquots were sampled into a graphite furnace and ICP-AES using a conventional autosampler. The method was validated by use of biological certified reference materials from NRC, DORM-2, DOLT-2, DOLT-3, LUTS-1, TORT-2, and NIST SRMs 1566b and 2976. Simplicity and reduced sample-preparation time prove to be the major advantages to the technique.
Scriver
Christine
C
Institute for National Measurement Standards, National Research Council of Canada, Ottawa, Ontario, K1A 0R9, Canada. Christine.scriver@nrc.ca
Kan
Masahiko
M
Willie
Scott
S
Soo
Catherine
C
Birnboim
H
H
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005561:Formates / Q000737:chemistry*; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods; D014131:Trace Elements / Q000032:analysis*
D005561:Formates; D014131:Trace Elements; C030544:formic acid