title: Assessment of microheterogeneity of blood flow in the rat urinary bladder by high-resolution digital radiography.
abstract: OBJECTIVE
  To assess high-resolution digital radiography for measuring blood flow and thus examine the microheterogeneity of bladder microcirculation in a rat model.
  
  
  METHODS
  Microheterogeneity of blood flow in both mucosa and detrusor muscle of eight anaesthetized rats was investigated using an imaging technique with very high spatial resolution (0.1 x 0.1 mm(2)) using digital radiography combined with the deposition of (3)H-labelled desmethylimipramine. The spatial pattern of blood flow was quantified by the coefficient of variation of the regional flow (CV = sd/mean). RESULTS Muscle blood flow was less than mucous blood flow (muscle : mucosa, 2.9 : 5) in the empty bladder. In the muscle layer the blood flow distribution was more heterogeneous than that in the mucosa, with a mean (sd) CV in muscle and mucosa of 0.33 (0.033) and 0.16 (0.019), respectively (P < 0.001) at the capillary level.
  
  
  CONCLUSIONS
  There was a heterogeneous distribution of blood flow in the microcirculation to capillary vessels in the muscular layer, possibly reflecting a difference in dynamic blood flow of regional perfusion of the emptied bladder.
authors.affiliation: Department of Urology, Kawasaki Medical School, 577 Matsushima, Kurashiki, 701-0192, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kimura
authors.forename: Tokunori
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sone
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takenaka
authors.forename: Masato
authors.initials: M
authors.lastname: Fujisawa
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D008297:Male; D008833:Microcirculation; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000098:blood supply* / Q000000981:diagnostic imaging