title: Effect of diesel exhaust on immune responses in C57BL/6 mice intranasally immunized with pollen antigen.
abstract: To investigate the effect of diesel exhaust or particle-free diesel gas on immune responses in IgE low responder mice, C57BL/6 mice immunized intranasally with sugi basic protein were exposed to diesel exhaust or diesel gas components. We evaluated the changes in lymphocyte subpopulations, cell proliferation, chemokine production of cervical lymph nodes cells, and antigen-specific-antibody levels in plasma. Exposure to diesel gas decreased the percentage of CD4+ and TCR-beta+ T cells of cervical lymph nodes from immunized mice. Culture supernatants of cervical lymph nodes cells from diesel gas-exposed mice had significantly increased levels of macrophage inflammatory protein-1alpha and thymus and activation-regulated chemokine, but exposure to diesel exhaust did not affect it. Antigen-specific IgG2a titers in plasma were significantly enhanced after their exposure to diesel exhaust or gas. In contrast, exposure to diesel exhaust or gas markedly decreased antigen-specific IgG1 titers in immunized mice. These facts indicate that concurrent exposure to allergen and diesel exhaust or diesel gas modulates chemokine production in cervical lymph nodes cells and antibody production in plasma differentially.
authors.affiliation: Environmental Health Sciences Division, National Institute for Environmental Studies, Onogawa, Tsukubashi, Ibaraki 305-8506, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Fujimaki
authors.forename: Shoji
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Yoshika
authors.initials: Y
authors.lastname: Kurokawa
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D028203:Cedrus / Q000276:immunology; D018925:Chemokines / Q000096:biosynthesis; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000276:immunology; D001335:Vehicle Emissions
chemical_list: D000941:Antigens; D018925:Chemokines; D001335:Vehicle Emissions; D007073:Immunoglobulin E