title: Inhibitory effects of the androgenic gland on ovarian development in the mud crab Scylla paramamosain.
abstract: Isolation and characterization of androgenic hormone in decapod crustaceans depend on an effective bioassay of its action. In the present study, the effect of androgenic gland on ovarian development in the mud crab Scylla paramamosain was investigated with a view to develop a bioassay for androgenic hormone. Ovarian regression with degeneration of oocytes occurred in some female crabs implanted with androgenic gland in vivo. In vitro incubation of ovarian tissues at secondary vitellogenesis in extract of androgenic gland resulted in a significant decrease in amino acid uptake by the tissues. We propose that this inhibitory effect could be established as an effective bioassay for the isolation of androgenic hormone in the mud crab.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Cui
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Ting Sze
authors.initials: TS
authors.lastname: Lo
authors.forename: Ka Hou
authors.initials: KH
authors.lastname: Chu
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D003386:Brachyura / Q000254:growth & development / Q000502:physiology*; D004702:Endocrine Glands / Q000737:chemistry / Q000502:physiology* / Q000637:transplantation*; D005260:Female; D005973:Glutamine / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D008297:Male; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012727:Sex Characteristics; D012733:Sex Differentiation / Q000502:physiology; D014020:Tissue Extracts / Q000494:pharmacology; D014818:Vitellogenesis
chemical_list: D000728:Androgens; D014020:Tissue Extracts; D005973:Glutamine