title: Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: confocal surface-enhanced Raman spectroscopic study.
abstract: Rapid and highly sensitive detection of duplex dye-labelled DNA sequences in a PDMS microfluidic channel was investigated using confocal surface enhanced Raman spectroscopy (SERS). This method does not need either an immobilization procedure or a PCR amplification procedure, which are essential for a DNA microarray chip. Furthermore, Raman peaks of each dye-labelled DNA can be easily resolved since they are much narrower than the corresponding broad fluorescence bands. To find the potential applicability of confocal SERS for sensitive bio-detection in a microfluidic channel, the mixture of two different dye-labelled (TAMRA and Cy3) sex determining Y genes, SRY and SPGY1, was adsorbed on silver colloids in the alligator teeth-shaped PDMS microfluidic channel and its SERS signals were measured under flowing conditions. Its major SERS peaks were observable down to the concentration of 10(-11) M. In the present study, we explore the feasibility of confocal SERS for the highly sensitive detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip.
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Taehan
authors.initials: T
authors.lastname: Park
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Gi Hun
authors.initials: GH
authors.lastname: Seong
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Yang S
authors.initials: YS
authors.lastname: Kim
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Ji
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
authors.forename: Dae-Gab
authors.initials: DG
authors.lastname: Gweon
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Lee
mesh_terms: D003102:Colloids / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D013499:Surface Properties
chemical_list: D003102:Colloids; D004396:Coloring Agents; D004129:Dimethylpolysiloxanes; D009841:Oligonucleotides; D012834:Silver; D004247:DNA