Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii.
Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5o and 3o ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.
Lin
Aifen
A
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
Shin
Eun-Hee
EH
Kim
Tae-Yun
TY
Park
Jae-Hwan
JH
Guk
Sang-Mee
SM
Chai
Jong-Yil
JY
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D017125:Genes, Protozoan; D005838:Genotype; D011110:Polymorphism, Genetic; D016053:RNA, Protozoan; D014122:Toxoplasma / Q000235:genetics*
D000953:Antigens, Protozoan; D016053:RNA, Protozoan