[Changes in the expression of apoptotic genes in the intestinal tissue of scalded rats before and after resuscitation].
OBJECTIVE
To explore the dynamic changes in the expression of c-fos, proliferation cell nuclear antigen (PCNA) and Bax in the intestinal tissue of scalded rats before and after resuscitation.


METHODS
Wistar rats inflicted with 30% TBSA full thickness scald were employed as the model and randomly divided into four groups with 8 in each group, i.e. 2.0, 2.5, 4.0, 6.0 postscald hour (PSH) groups. Rats in each group received routine fluid infusion at 2 PSH, and were sacrificed at 2, 2.5, 4, 6 PSH, respectively. Then the intestinal tissue of the rats was harvested for the detection of the expression of c-fos, PCNA and Bax.


RESULTS
The expression of c-fos, PCNA and Bax at 2.0 PSH group (65.8 +/- 4.2%, 74.5 +/- 2.4%, 26.3 +/- 5.7%, respectively) significantly increased when compared with those in 2.5 PSH group (92.4 +/- 5.7%, 85.6 +/- 4.5%, 67.1 +/- 6.6%, respectively) (P < 0.01). The expression of 3 genes increased dramatically at 2.5 and 4.0 PSH, and reached the peak at 2.5 PSH. There was no obvious difference in the gene expression between 4 PSH and 2 PSH groups.


CONCLUSIONS
The expression of apoptotic genes in the intestinal tissue of scalded rats increased significantly during early resuscitation stage after burn injury.
Chen
Jing
J
Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100041, P.R. China.
Wang
Jia-Han
JH
Ren
Jia-Liang
JL
D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000378:metabolism* / Q000473:pathology*; D015870:Gene Expression; D016762:Genes, fos; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D012774:Shock, Traumatic; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494828:Bax protein, rat; D018809:Proliferating Cell Nuclear Antigen; D051028:bcl-2-Associated X Protein