Molecular analysis of primary and recurrent giant cell tumors of bone.
The status of microsatellite markers located on chromosomes 1p36, 3p25, 5q23, 9p22, 10q23, 10q24, 17p13, and 19q12 was used to determine loss of heterozygosity (LOH) in primary giant cell tumors (GCT) of bone in 12 patients. The cases included primary, locally recurrent, and metastatic GCT; three tumors were classified as malignant GCT, based on their morphological features. Microdissection was performed on 24 paraffin-embedded tissue samples. An average of three separate topographic sites were microdissected from each tumor. Case selection in each instance was based on the availability of paired samples of tumor in primary GCTs and their corresponding recurrences, and the presence of normal tissue. The number of cases studied is too small for statistical studies, and thus the analysis is descriptive. All cases were informative for >80% of the markers used. Both primary GCTs and local recurrences and lung metastases displayed LOH of three or more markers, and intratumoral heterogeneity was frequent. Fractional allelic losses (FAL) were not different in recurrent and nonrecurrent GCT. FAL was greatest (>30%) in the metastatic group of GCT. Allelic losses of 1p, 9q, and 19q regions were frequent in all groups. LOH of 17p (in proximity to the p53 locus) and 9p occurred exclusively in the pulmonary metastases from GCT. LOH of 9q and 19q was present in primary as well as recurrent GCTs and in one malignant GCT. Involvement of 1p (including MYCL) and 9q regions has not been previously reported in GCT of bone. The pattern of LOH evident in the 17 markers used in the present study suggests that GCT with malignant features may follow an evolutionary pathway similar to the usual primary GCT of bone.
Rao
Uma N M
UN
Department of Pathology, University of Pittsburgh Medical Center-Presbyterian Shadyside, Room WG02.9, 5230 Centre Avenue, Pittsburgh, PA 15213, USA. raounm@upmc.edu
Goodman
Mark
M
Chung
Wen-Wei
WW
Swalski
Patricia
P
Pal
Raj
R
Finkelstein
Sydney
S
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D005260:Female; D005819:Genetic Markers; D005870:Giant Cell Tumors / Q000000981:diagnostic imaging / Q000235:genetics* / Q000473:pathology; D006801:Humans; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D042282:Microdissection; D018895:Microsatellite Repeats; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local / Q000473:pathology*; D016133:Polymerase Chain Reaction; D011859:Radiography
D005819:Genetic Markers