Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae.
Various selection procedures in chemostats and batch cultures were systematically tested for their efficiency to select for a multiple-stress resistance phenotype in Saccharomyces cerevisiae. To determine the relative stress resistance phenotypes, mutant populations harvested at different time points and randomly chosen clones from selected populations were grown in batch cultures and exposed to oxidative, freezing-thawing, high-temperature and ethanol stress. For this purpose, we developed a high-throughput procedure in 96-well plates combined with a most-probable-number assay. Among all chemostat and batch selection strategies tested, the best selection strategy to obtain highly improved multiple-stress-resistant yeast was found to be batch selection for freezing-thawing stress. The final mutant populations selected for this particular stress were not only significantly improved in freezing-thawing stress resistance, but also in other stress resistances. The best isolated clone from these populations exhibited 102-, 89-, 62-, and 1429-fold increased resistance to freezing-thawing, temperature, ethanol, and oxidative stress, respectively. General selection guidelines for improving multiple-stress resistance in S. cerevisiae are presented and discussed.
Cakar
Z Petek
ZP
Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, TR-34469 Istanbul, Turkey. cakarp@itu.edu.tr
Seker
Urartu O S
UO
Tamerler
Candan
C
Sonderegger
Marco
M
Sauer
Uwe
U
D003470:Culture Media; D019020:Directed Molecular Evolution; D000431:Ethanol / Q000494:pharmacology; D005615:Freezing; D005818:Genetic Engineering / Q000379:methods*; D018869:Heat-Shock Response; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D012641:Selection, Genetic
D003470:Culture Media; D000431:Ethanol