title: Heterologous protein production from the inducible MET25 promoter in Saccharomyces cerevisiae.
abstract: Heterologous protein production late in Saccharomyces cerevisiae fermentations is often desirable because it may help avoid the unintentional selection of more rapidly growing, non-protein-expressing cells or allow for the expression of toxic proteins. Here, we describe the use of the MET25 promoter for the production of human serum albumin (HSA) and HSA-fusion proteins in S. cerevisiae. In media lacking methionine, the MET25 promoter yielded high expression levels of HSA and HSA fused to human glucagon, human growth hormone, human interferon alpha, and human interleukin-2. More importantly, we have shown that this system can be used to delay heterologous protein production until late log phase of the growth of the culture and does not require the addition of an exogenous inducer.
authors.affiliation: Human Genome Sciences, Inc., Rockville, Maryland 20850, USA. solow@ncbi.nlm.nih.gov
authors.forename: Steven P
authors.initials: SP
authors.lastname: Solow
authors.forename: Jennifer
authors.initials: J
authors.lastname: Sengbusch
authors.forename: Michael W
authors.initials: MW
authors.lastname: Laird
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D008715:Methionine