title: Molecular mechanisms by which rRNA mutations confer resistance to clindamycin.
abstract: The mechanisms by which rRNA mutations confer clindamycin resistance were examined in Mycobacterium smegmatis strains containing homogeneous populations of ribosomes with base substitutions at nucleotides A2058 and A2059. Computer graphic predictions based on structural studies correlate with the resistance phenotypes for six of seven strains with unique rRNA mutations.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. jacobp@bmb.sdu.dk
authors.forename: Jacob
authors.initials: J
authors.lastname: Poehlsgaard
authors.forename: Peter
authors.initials: P
authors.lastname: Pfister
authors.forename: Erik C
authors.initials: EC
authors.lastname: Böttger
authors.forename: Stephen
authors.initials: S
authors.lastname: Douthwaite
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D002981:Clindamycin