Diversity of ectomycorrhizal fungi naturally established on containerised Pinus seedlings in nursery conditions.
The study examined the diversity of ectomycorrhizal fungi, naturally established on roots of containerised Pinus seedlings in a nursery, using PCR-RFLP and sequencing of the nuclear ribosomal internal transcribed spacer. Seventy-two samples, including ectomycorrhizae and fruit bodies, were examined. Molecular typing assigned the fungal symbionts to four ectomycorrhizal Boletales: Rhizopogon rubescens, Suillus bovinus, S. variegatus, and R. luteolus. R. rubescens was abundant (37.5%), while Suillus and R. luteolus species were moderately established (25-26%) and rare (2.8%), respectively. In addition, Rhizopogon species colonised P. nigra ssp. salzmannii seedlings, whereas Suillus species were identified on Pinus nigra ssp. nigra seedlings. The diversity and the ability of these naturally established symbionts under artificial nursery conditions were discussed. The molecular survey investigated here should contribute to successful monitoring of mycorrhizal application under both nursery and plantation conditions.
El Karkouri
Khalid
K
UMR INRA-ENSA.M Sol et Environnement, 2, Place Viala, 34060 Montpellier Cedex 1, France. khalid.2@wanadoo.fr
Martin
Francis
F
Douzery
J P Emmanuel
JP
Mousain
Daniel
D
D001487:Basidiomycota / Q000235:genetics / Q000302:isolation & purification*; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D038821:Mycorrhizae / Q000235:genetics / Q000302:isolation & purification*; D028223:Pinus / Q000254:growth & development / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology*; D013045:Species Specificity; D013559:Symbiosis
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer