title: Perfluorohexane vapor has only minor effects on spatial pulmonary blood flow distribution in isolated rabbit lungs.
abstract: We tested the hypothesis that administration of perfluorohexane (PFH) vapor does not significantly affect the relative pulmonary blood flow (Qrel) distribution in isolated rabbit lungs. Fourteen isolated rabbit lungs were perfused with a Krebs-Henseleit buffer solution (flow 150 mL/min). Pulmonary afterload was set to 3 mm Hg. The lungs were ventilated with 4% CO(2) in room air using a small animal ventilator (respiratory rate, 30 breaths/min; tidal volume, 12 mL/kg body weight; positive end-expiratory pressure, 2 cm H(2)O). After a steady-state period, 18 vol. % of PFH vapor was administered to 9 lungs for 30 min. In a second set of experiments five lungs served as controls. Change in (Qrel) distribution was assessed using fluorescent-labeled microspheres. The unpaired Student's t-test was used to compare variables between groups. The paired Student's t-test, the one-sample Student's t-test, the Anderson-Hauck test of equivalence, and Pearson correlation were used to analyze changes within groups. The mean correlation coefficients of (Qrel) were 0.564 +/- 0.182 for the PFH group and 0.502 +/- 0.295 for the control group, respectively. No significant changes in (rel) distribution over time and between groups were found. However, in the PFH group a tendency towards redistribution of (Qrel) to more ventral lung areas was noted. Our results suggest that PFH vapor has no significant effects on redistribution of (Qrel) in isolated rabbit lungs.
authors.affiliation: From the Department of Anesthesiology and Intensive Care Medicine, Technical University Dresden, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hübler
authors.forename: Axel R
authors.initials: AR
authors.lastname: Heller
authors.forename: Jörg U
authors.initials: JU
authors.lastname: Bleyl
authors.forename: Marcelo Gama
authors.initials: MG
authors.lastname: de Abreu
authors.forename: Tobias
authors.initials: T
authors.lastname: Kroll
authors.forename: Thomas
authors.initials: T
authors.lastname: Rössel
authors.forename: Thea
authors.initials: T
authors.lastname: Koch
mesh_terms: D000465:Algorithms; D000818:Animals; D002682:Chinchilla; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000502:physiology; D008297:Male; D009929:Organ Size; D011652:Pulmonary Circulation / Q000187:drug effects*; D011659:Pulmonary Gas Exchange; D011817:Rabbits
chemical_list: D005466:Fluorocarbons; C078626:perflexane