Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles.
Cytotoxicity of CdSe and CdSe/ZnS nanoparticles has been investigated for different surface modifications such as coating with mercaptopropionic acid, silanization, and polymer coating. For all cases, quantitative values for the onset of cytotoxic effects in serum-free culture media are given. These values are correlated with microscope images in which the uptake of the particles by the cells has been investigated. Our data suggest that in addition to the release of toxic Cd(2+) ions from the particles also their surface chemistry, in particular their stability toward aggregation, plays an important role for cytotoxic effects. Additional patch clamp experiments investigate effects of the particles on currents through ion channels.
Kirchner
Christian
C
Center for Nanoscience, Ludwig Maximilians Universität München, Germany.
Liedl
Tim
T
Kudera
Stefan
S
Pellegrino
Teresa
T
Muñoz Javier
Almudena
A
Gaub
Hermann E
HE
Stölzle
Sonja
S
Fertig
N
N
Parak
Wolfgang J
WJ
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000493:pharmacokinetics; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D016466:CHO Cells; D019187:Cadmium Compounds / Q000009:adverse effects* / Q000493:pharmacokinetics*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids / Q000009:adverse effects / Q000493:pharmacokinetics; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008422:Materials Testing; D043942:Nanotubes / Q000009:adverse effects*; D018570:Risk Assessment / Q000379:methods; D012307:Risk Factors; D018036:Selenium Compounds / Q000009:adverse effects* / Q000493:pharmacokinetics*; D013440:Sulfides / Q000009:adverse effects* / Q000493:pharmacokinetics*; D017967:Zinc Compounds / Q000009:adverse effects* / Q000493:pharmacokinetics*
D001672:Biocompatible Materials; D019187:Cadmium Compounds; D003102:Colloids; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide