Stable maintenance and expression of a foreign gene in transgenic pear shoots retrieved from in vitro conservation.
Shoot-tips of transgenic pear were successfully conserved in vitro by slow-growth and cryopreservation methods. After 1 year of storage in slow growth conditions, all shoot-tips survived showing shoot re-growth. Similarly, shoot-tips showed high survival and regeneration rates after cryopreservation. The genetic stability of the transgenic GUS construct in shoots recovered from slow-growth and cryopreservation was assessed. The results from polymerase chain reaction and Southern blotting showed the stable maintenance of the GUS gene in genome, and the Single-Strand Conformation Polymorphism assay did not detect single base variation. X-Gluc staining suggested a normal expression of the GUS gene in shoots retrieved from these storage methods.
Hao
Yu-Jin
YJ
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, PR China. yjcxhao@hotmail.com
Cheng
Yun-Jiang
YJ
Deng
Xiu-Xin
XX
D015139:Blotting, Southern; D015925:Cryopreservation; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D066298:In Vitro Techniques; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D031989:Pyrus / Q000235:genetics* / Q000254:growth & development