title: Corticotropin-releasing factor mRNA and substance P receptor binding in the paraventricular hypothalamic nucleus, central nucleus of the amygdala, and locus coeruleus of Sprague-Dawley rats following restraint-induced stress.
abstract: The central mechanism of stress is poorly understood. This study was designed to examine how corticotropin-releasing factor (CRF) neurons, together with substance P (SP) receptors in the paraventricular hypothalamic nucleus (PVN), central nucleus of the amygdala (CeA), and locus coeruleus (LC), are affected by stress. Sprague-Dawley rats were restrained for 2 h. Animals were sacrificed by decapitation immediately after the 2-h restraint (the 0-h group) and 4, 24, or 48 h after restraint. Tissue sections were cut and collected on two sets of slides. Tissue sections of the first set were processed for studying CRF mRNA using 33P-labeled 60-mer oligonucleotide probe. Immediately adjacent tissue sections were processed for studying SP receptor-binding capacity using 125I-SP ligand. Quantitative results showed that CRF mRNAs in the PVN were significantly up-regulated at the 4- and 24-h stages, and they seemed not to be regulated by SP receptors. In addition, SP receptors in the CeA were up-regulated at the 24- and 48-h stages, whereas SP receptors were down-regulated in the LC at the same stages. In concert with the literature indicating SP antagonist's antidepressive effects, up-regulated SP receptors in the CeA might contribute to the development of stress-related depression.
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. bhwang@iupui.edu
authors.forename: Bang H
authors.initials: BH
authors.lastname: Hwang
authors.forename: Jason
authors.initials: J
authors.lastname: Katner
authors.forename: Smriti
authors.initials: S
authors.lastname: Iyengar
mesh_terms: D000679:Amygdala / Q000502:physiology; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism*; D005243:Feces; D008125:Locus Coeruleus / Q000502:physiology; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D003346:Corticotropin-Releasing Hormone