Drought-inducible-but ABA-independent-thaumatin-like protein from carrot (Daucus carota L.).
Drought treatment induces the accumulation of dcTLP, which is similar in structure to the thaumatin-like proteins (TLPs) found in the embryogenic calli, seedlings, and mature plants of carrot (Daucus carota). We isolated a full-length dcTLP cDNA clone from carrot and characterized the 5' upstream sequences. The coding region of dcTLP consisted of 645 nucleotides; the theoretical pI value was 4.9, and its molecular weight was approximately 22 kDa. The production of dcTLP transcripts in the seedlings increased dramatically with dehydration treatment but was not affected by abscisic acid (ABA), salicylic acid, or jasmonic acid. The expression patterns of dcTLP mRNA at different developmental stages and in response to a variety of signal molecules was analyzed using reverse transcriptase-PCR and promoter analysis with fused genes of 0.5-kb 5' upstream sequences in which beta-glucuronidase (GUS) reporter genes (gus) were established. The induction of dcTLP was found to be highly specific to drought stress in the embryogenic calli, seedlings, and mature plants. Our results suggest that this new isoform of TLP that has been isolated from carrot is a drought-specific, ABA-independent, non-organ-specific, and non-developmental-stage-specific protein.
Jung
Y C
YC
Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.
Lee
H J
HJ
Yum
S S
SS
Soh
W Y
WY
Cho
D Y
DY
Auh
C K
CK
Lee
T K
TK
Soh
H C
HC
Kim
Y S
YS
Lee
S C
SC
D000595:Amino Acid Sequence / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D018552:Daucus carota / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003681:Dehydration / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
C092226:ATLP-1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010940:Plant Proteins; D012333:RNA, Messenger