Hydroxyl radical production and oxidative damage induced by cadmium and naphthalene in liver of Carassius auratus.
Freshwater goldfish (Carassius auratus) were exposed to cadmium (Cd) from 0 to 5 mg/L, and naphthalene (NAP) from 0 to 50 mg/L. Twenty-four hours after the exposure, reactive oxygen species (ROS) was trapped by phenyl-tert-butyl nitrone and detected by electron paramagnetic resonance (EPR). Protein carbonyl (PCO) and lipid peroxidation (LPO) content were determined. The activities of superoxide dismutase (SOD) and catalase (CAT) were also measured. The EPR spectra signals were characterized by prominent six-line spectra, which were defined as hydroxyl radical ((.)OH). As compared to the control group, Cd and NAP significantly induced (.)OH production marked by the intensity of the prominent spectra at higher concentrations. Both xenobiotics also increased LPO content and PCO content, depending on the concentrations. Either LPO or PCO content showed significant relation with (.)OH production. Cd increased the activity of SOD and decreased that of CAT at 5 mg/L, and NAP increased the activities of SOD and CAT at 5 mg/L. The results clearly indicated that these two structurally different non-redox cycling xenobiotics could induce (.)OH generation and result in oxidative damage in liver of C. auratus, and these effects were concentration-dependent.
Shi
Huahong
H
State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, China.
Sui
Yunxia
Y
Wang
Xiaorong
X
Luo
Yi
Y
Ji
Liangliang
L
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006054:Goldfish / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009281:Naphthalenes / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology
D009281:Naphthalenes; D002104:Cadmium; C031721:naphthalene; D017665:Hydroxyl Radical