Ellagic acid inhibits growth and arylamine N-acetyltransferase activity and gene expression in Staphylococcus aureus.
It is well documented that N-acetyltransferase (NAT) plays a key role in the N-acetylation of arylamine compounds. Ellagic acid was demonstrated to elicit dose-dependent bacteriostatic activity and inhibition of N-acetylation of 2-aminofluorene (AF). N-acetylation of AF in S. aureus was determined by high preformance liquid chromatography. The apparent values of Km and Vmax of NAT were decreased after co-treatment with 0.5 mM ellagic acid in the cytosol of S. aureus. PCR also indicated that ellagic acid inhibited NAT gene expression (NAT mRNA) in S. aureus.
Lu
Hsu-Feng
HF
Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan.
Tsou
Mei-Fen
MF
Lin
Jaung-Gung
JG
Ho
Chin-Chin
CC
Liu
Jia-You
JY
Chuang
Jing-Yuan
JY
Chung
Jing-Gung
JG
D000107:Acetylation / Q000187:drug effects; D001191:Arylamine N-Acetyltransferase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004610:Ellagic Acid / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005449:Fluorenes / Q000032:analysis / Q000037:antagonists & inhibitors; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006131:Growth Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
D004791:Enzyme Inhibitors; D005449:Fluorenes; D006131:Growth Inhibitors; D004610:Ellagic Acid; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase