title: [No evidence for genetic association between alpha-2 macroglobulin I1000V polymorphism and sporadic Alzheimer's disease in two independent Chinese populations].
abstract: OBJECTIVE
  Alpha-2 macroglobulin (alpha2M) is a proteinase inhibitor found in association with senile plaques in Alzheimer's disease (AD). Also alpha2M has been implicated in several pathophysiological processes in AD. In view of the recent contradictory reports on the relationship between AD and a common polymorphism I1000V in A2M gene, the present authors studied a relatively large sample, determined the genotype of the I1000V polymorphism in A2M gene in sporadic AD patients and age-matched controls with normal cognition, and examined the possible association of the polymorphism with AD.
  
  
  METHODS
  Genotypes of A2M and apolipoprotein E (apoE) were detected by polymerase chain reaction combined with restriction fragment length polymorphism in 257 patients and 242 controls in Guangzhou, and 112 patients and 113 controls in Chengdu.
  
  
  RESULTS
  The 1000Val allele frequencies in the merged AD and control groups were 7.7% and 8.7%, respectively. The differences of allelic and genotypic frequencies between the patients and control subjects were not statistically significant, even after stratification by apoE epsilon4 status or by age-of-onset of the disease.
  
  
  CONCLUSIONS
  The results of this study revealed no association between the I1000V polymorphism of A2M and Chinese sporadic AD in Guangzhou and Chengdu.
authors.affiliation: Department of Medical Genetics, West China Hospital, Sichuan University, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Chengdu, Sichuan, 610041 P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Jiajun
authors.initials: J
authors.lastname: Shi
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.forename: Mouni
authors.initials: M
authors.lastname: Tang
authors.forename: Xiehe
authors.initials: X
authors.lastname: Liu
authors.forename: Yingcheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Haiying
authors.initials: H
authors.lastname: Han
authors.forename: Yangbo
authors.initials: Y
authors.lastname: Guo
authors.forename: Hehuang
authors.initials: H
authors.lastname: Deng
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhao
authors.forename: Cui
authors.initials: C
authors.lastname: Ma
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000208:ethnology / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000511:alpha-Macroglobulins / Q000235:genetics*
chemical_list: D001057:Apolipoproteins E; D000511:alpha-Macroglobulins