title: 2,4-Dinitrophenol pharmacologically promotes retinal detachment in rabbits.
abstract: OBJECTIVE
  The most difficult and unpredictable step of macular translocation surgery is creating the retinal detachment. The authors evaluated the efficacy of 2,4-dinitrophenol (2,4-DNP) to promote retinal detachment in the rabbit.
  
  
  METHODS
  A vitrectomy was performed in each eye of a Dutch-belted rabbit. One eye was injected with 0.1 cc of a 5 mmol/L 2,4-DNP, the other eye with 0.1 cc of BSS+. After 30 minutes, the minimum aspiration pressure required to visibly elevate the retina was recorded. Four nonvitrectomized eyes received an intravitreal injection of either 0.1 cc of BSS+ or 5 mmol/L 2,4-DNP, and were enucleated and fixated for histopathologic examination.
  
  
  RESULTS
  In the 12 masked eyes, the mean aspiration pressure decreased from 217 +/- 20 mmHg in the six BSS+ eyes to 117 +/- 20 mmHg in the six 2,4-DNP treated eyes (P = 0.0022). A retinal detachment was present in three of six masked and two of two unmasked 2,4-DNP treated eyes and none of eight BSS+ treated eyes. There was no short-term toxicity to the retina at the light microscope level.
  
  
  CONCLUSIONS
  Intravitreal injection of 2,4-DNP reduced the retinal adhesive force by over 50% when compared to the BSS+ treated control eyes, without any short-term retinal toxicity.
authors.affiliation: Department of Ophthalmology, University of Maryland, Baltimore, USA.
authors.forename: Ahad
authors.initials: A
authors.lastname: Deen
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Benner
authors.forename: Theresa
authors.initials: T
authors.lastname: Kramer
authors.forename: Karen
authors.initials: K
authors.lastname: Bassichis-Saland
authors.forename: Amish
authors.initials: A
authors.lastname: Purohit
authors.forename: Scott M
authors.initials: SM
authors.lastname: Steidl
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology*; D000818:Animals; D022001:Focal Adhesions / Q000187:drug effects; D007267:Injections; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000473:pathology / Q000637:transplantation; D012163:Retinal Detachment / Q000139:chemically induced* / Q000473:pathology; D014475:Uncoupling Agents / Q000494:pharmacology*; D014821:Vitrectomy
chemical_list: D014475:Uncoupling Agents; D019297:2,4-Dinitrophenol