Frequent overexpression of cyclin D2/cyclin-dependent kinase 4 in Wilms' tumor.
The expression status of the three cyclin D genes (CCND1, CCND2 and CCND3), the two cyclin D-dependent kinase genes (CDK4 and CDK6) and the p16(INK4a) gene was studied in a series of 47 Wilms' tumors, 16 normal mature kidneys and two fetal kidneys. We showed predominant overexpression of CCND2 and CDK4 compared to CCND1/D3 and CDK6 respectively. We found a specific correlation between relapse and CDK4 overexpression, but not CDK6 overexpression. We did not identify any methylation of the p16(INK4a) promoter. This suggests that dysregulation of CCND2 and CDK4 plays a specific role in WT tumorigenesis.
Faussillon
Marine
M
INSERM U383, Hôpital Necker-Enfants Malades, Université René Descartes, 149-161 rue de Sèvres, 75743 Paris Cedex 15, France.
Monnier
Lucie
L
Junien
Claudine
C
Jeanpierre
Cécile
C
D019938:Cyclin D1 / Q000235:genetics; D056742:Cyclin D2; D051358:Cyclin-Dependent Kinase 4; D051361:Cyclin-Dependent Kinase 6; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D016213:Cyclins / Q000235:genetics*; D019175:DNA Methylation; D006801:Humans; D007668:Kidney / Q000196:embryology / Q000201:enzymology; D007680:Kidney Neoplasms / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
C080336:CCND2 protein, human; D056742:Cyclin D2; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D011518:Proto-Oncogene Proteins; D019938:Cyclin D1; C495900:CDK4 protein, human; C495911:CDK6 protein, human; D051358:Cyclin-Dependent Kinase 4; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases