Aspergillus vadensis, a new species of the group of black Aspergilli.
A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the beta-tubulin sequences of the individual strains. The new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone B, nigragillin, asperazine and kotanins. RFLP analysis using three genes as probes also resulted in a unique pattern. These data indicate that the strain was closely related but not identical to Aspergillus foetidus, Aspergillus niger and Aspergillus tubingensis. It was therefore designated as a novel species and named Aspergillus vadensis.
de Vries
Ronald P
RP
Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, 6703 HA, Wageningen, The Netherlands. r.p.devries@bio.uu.nl
Frisvad
Jens C
JC
van de Vondervoort
Peter J I
PJ
Burgers
Kim
K
Kuijpers
Angelina F A
AF
Samson
Robert A
RA
Visser
Jaap
J
D001230:Aspergillus / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D007211:Indoles / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D010879:Piperazines / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D006603:Hexuronic Acids; D007211:Indoles; D010879:Piperazines; D014404:Tubulin; C437185:asperazine; C007819:galacturonic acid; D005990:Glycerol