title: Chiral speciation and determination of DL-selenomethionine enantiomers on a novel chiral ligand-exchange stationary phase.
abstract: A new type of chiral ligand-exchange stationary phase (CLES) was successfully synthesized by treating silica gel with beta-(3,4-epoxycyclohexyl)ethyltrimethoxy silane and opening the epoxy ring by L-isoleucine. The chiral speciation of DL-selenomethionine (DL-SeMet) by high-performance liquid chromatography (HPLC) with UV absorbance on the CLES column was studied. The influences of the contents of copper ion and methanol as well as the pH value in the mobile phase and temperature of the column on the efficiency of resolution of DL-SeMet were investigated in detail. DL-SeMet could be completely resolved within 40 min under the optimal operating conditions of 0.1 mmol/L Cu2+ at 0.05 mol/L KH2PO4 buffer (pH = 5.5) and 35 degrees C temperature of the column. The limits of detection of D- and L-SeMet were 255 ppb and 286 ppb, respectively. This method was applied to determine the D- and L-enantiomers of DL-SeMet in real samples, such as selenized yeast powder and garlic.
authors.affiliation: Department of Chemistry, MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China. hxj@yanan.xmu.edu.cn
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Huang
authors.forename: Junde
authors.initials: J
authors.lastname: Wang
authors.forename: Qiuquan
authors.initials: Q
authors.lastname: Wang
authors.forename: Benli
authors.initials: B
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005737:Garlic / Q000737:chemistry; D008024:Ligands; D015394:Molecular Structure; D012645:Selenomethionine / Q000032:analysis* / Q000737:chemistry*; D013237:Stereoisomerism; D015003:Yeasts / Q000737:chemistry
chemical_list: D008024:Ligands; D012645:Selenomethionine