Solid-supported synthesis of polymerizable lanthanide-ion chelating lipids for protein detection.
Usually, lipids are synthesized employing solution-phase organic synthesis techniques. Though successful, the purifications can be difficult to accomplish due to the amphiphilic nature of the molecules. Herein, we demonstrate the advantages of a solid-phase approach for preparing a variety of metal-chelating lipids. A number of saturated and polymerizable metal-chelating lipids were prepared using this methodology. This approach requires one chromatographic purification after cleaving the lipids from the solid support. We also demonstrate that the resulting polymerized liposomes (containing Eu3+) possess the appropriate luminescence properties for the qualitative and quantitative determination of proteins.
Nadi
Suad
S
Department of Chemistry and Molecular Biology, North Dakota State University, Fargo, North Dakota 58105, USA.
Santos
Marina
M
Haldar
Manas K
MK
Roy
Bidhan C
BC
Mallik
Sanku
S
Campiglia
Andres D
AD
D002614:Chelating Agents / Q000138:chemical synthesis*; D005063:Europium / Q000737:chemistry*; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008055:Lipids / Q000138:chemical synthesis*; D008081:Liposomes / Q000138:chemical synthesis*; D008163:Luminescent Measurements; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis*
D002614:Chelating Agents; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements; D008055:Lipids; D008081:Liposomes; D011506:Proteins; D005063:Europium