A custom microarray platform for analysis of microRNA gene expression.
MicroRNAs are short, noncoding RNA transcripts that post-transcriptionally regulate gene expression. Several hundred microRNA genes have been identified in Caenorhabditis elegans, Drosophila, plants and mammals. MicroRNAs have been linked to developmental processes in C. elegans, plants and humans and to cell growth and apoptosis in Drosophila. A major impediment in the study of microRNA function is the lack of quantitative expression profiling methods. To close this technological gap, we have designed dual-channel microarrays that monitor expression levels of 124 mammalian microRNAs. Using these tools, we observed distinct patterns of expression among adult mouse tissues and embryonic stem cells. Expression profiles of staged embryos demonstrate temporal regulation of a large class of microRNAs, including members of the let-7 family. This microarray technology enables comprehensive investigation of microRNA expression, and furthers our understanding of this class of recently discovered noncoding RNAs.
Thomson
J Michael
JM
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Parker
Joel
J
Perou
Charles M
CM
Hammond
Scott M
SM
D000818:Animals; D002460:Cell Line; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D013234:Stem Cells / Q000502:physiology
D035683:MicroRNAs