title: Use of housekeeping gene sequencing for species identification of viridans streptococci.
abstract: Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis-sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Pattarachai
authors.initials: P
authors.lastname: Kiratisin
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Murray
authors.forename: Steven H
authors.initials: SH
authors.lastname: Fischer
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005941:Glucokinase / Q000235:genetics*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D034363:Viridans Streptococci / Q000145:classification* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005954:Glucosephosphate Dehydrogenase; D005941:Glucokinase