Protective effect of diphenyl diselenide on acute liver damage induced by 2-nitropropane in rats.
The effect of diphenyl diselenide, (PhSe)2, administration on 2-nitropropane (2-NP)-induced hepatic damage was examined in male rats. Rats were pre-treated with a single dose of diphenyl diselenide (10, 50 or 100 micromol/kg). Afterward, they received only one dose of 2-NP (100 mg/kg body weight dissolved in olive oil). The parameters that indicate tissue damage such as plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alpha-fetoprotein (AFP), creatinine and urea were determined. Since toxicity induced by 2-NP is related to oxidative stress, lipid peroxidation was also evaluated. Diphenyl diselenide (100 micromol/kg) significantly reduced plasma ALT, gamma-GGT, AFP levels when compared to 2-NP group. Treatment with diphenyl diselenide, at all doses, effectively protects the increase of lipid peroxidation when compared to 2-NP group. Histological examination revealed that 2-NP treatment causes a moderate swelling and degenerative alterations on hepatocytes and diphenyl diselenide (100 micromol/kg) protects against these alterations. Diphenyl diselenide (50 and 100 micromol/kg) significantly decreased the urea level. This study evidences the protective effect of diphenyl diselenide by 2-NP-induced acute hepatic damage.
Borges
Lysandro P
LP
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil.
Borges
Vanessa Corralo
VC
Moro
Angelica Venturini
AV
Nogueira
Cristina Wayne
CW
Rocha
Joao Batista Teixeira
JB
Zeni
Gilson
G
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001555:Benzene Derivatives / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control; D003404:Creatinine / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009595:Nitroparaffins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D011407:Propane / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000097:blood
D001555:Benzene Derivatives; D009595:Nitroparaffins; D016566:Organoselenium Compounds; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D000509:alpha-Fetoproteins; C061132:diphenyldiselenide; D003404:Creatinine; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C016617:2-nitropropane; D011407:Propane