Acamprosate involvement in triacylglycerol hydrolysis and transacylation with cholesterol in chronically ethanol-drinking rats.
Acamprosate (AC) is used as a drug for treating alcoholism. We evaluated the effect of AC on serum triacylglycerol hydrolysis (GEH, glycerol ester hydrolysis), triacylglycerol transacylation with cholesterol (GECAT, glycerol ester:cholesterol acyltransferase), and acylcholesterol hydrolysis (Cease, cholesterol ester hydrolysis) in an experimental model of alcoholism. Ethanol-preferring (PRF), non-preferring (NPF), and control (CR) male Wistar rats were treated with AC (500 mg/kg, p.o.) for 21 consecutive days. The beneficial effect of AC on lipid parameters of PRF rats included decreased triacylglycerol, total cholesterol, and LDL-cholesterol, and increased HDL-cholesterol levels. Acamprosate-compensated changes associated with ethanol consumption were observed. Acamprosate treatment decreased GECAT and increased Cease control rats, but increased GECAT and decreased CEase in PRF animals. In all groups of rats, AC treatment did not influence GEH. In conclusion, our results suggest that AC can influence triacylglycerol metabolism by its action on the balance between hydrolysis and transacylation in rats.
Piorunska-Mikolajczak
Anna
A
Department of General Chemistry Poznan University of Medical Sciences Grunwaldzka 6, 60- 780 Poznan, Poland. aspm@amp.edu.pl
Piorunska-Stolzmann
Maria
M
Mikolajczak
Przemyslaw
P
Okulicz-Kozaryn
Irena
I
Kaminska
Ewa
E
D000077443:Acamprosate; D000215:Acylation / Q000187:drug effects; D000427:Alcohol Deterrents / Q000494:pharmacology* / Q000627:therapeutic use; D000428:Alcohol Drinking / Q000097:blood* / Q000517:prevention & control; D000818:Animals; D002784:Cholesterol / Q000097:blood*; D000431:Ethanol / Q000008:administration & dosage*; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D002785:Sterol O-Acyltransferase / Q000097:blood; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use; D014280:Triglycerides / Q000097:blood*
D000427:Alcohol Deterrents; D014280:Triglycerides; D013654:Taurine; D000431:Ethanol; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; D000077443:Acamprosate