Cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase activity.
This communication describes a design of cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase expression in living mammalian cells. This design is based on fluorescence energy transfer resonance and utilizes a peracetylated d-glucosamine to facilitate the transport of the near-infrared probe across cell membranes. This new type of fluorogenic probe may also be applied to image gene expression in living animals.
Xing
Bengang
B
Department of Radiology, Biophysics, Bio-X, Cancer Biology, and Molecular Imaging Programs at Stanford, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5344, USA.
Khanamiryan
Ashot
A
Rao
Jianghong
J
D000107:Acetylation; D000818:Animals; D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D002511:Cephalosporins / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D005910:Glioma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D051381:Rats; D019265:Spectroscopy, Near-Infrared; D014162:Transfection; D001618:beta-Lactamases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D002232:Carbocyanines; D002511:Cephalosporins; D005456:Fluorescent Dyes; C085321:cyanine dye 5; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; D005944:Glucosamine