title: Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei.
abstract: Class D beta-lactamase OXA-57 was identified in a range of isolates of Burkholderia pseudomallei and Burkholderia thailandensis. Comparative kinetic analyses of wild-type and mutant forms of B. pseudomallei OXA-57 are reported. Implications of these data for beta-lactam resistance and the proposed role of Ser-104 in beta-lactam hydrolysis are discussed.
authors.affiliation: Department of Biological Sciences, CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Keith
authors.forename: Petra C
authors.initials: PC
authors.lastname: Oyston
authors.forename: Ben
authors.initials: B
authors.lastname: Crossett
authors.forename: Neil F
authors.initials: NF
authors.lastname: Fairweather
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Walsh
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D019117:Burkholderia / Q000187:drug effects / Q000201:enzymology*; D016957:Burkholderia pseudomallei / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases