title: Human palatal saliva: adsorption behaviour and the role of low-molecular weight proteins.
abstract: In situ ellipsometry was employed to study adsorption from human palatal saliva (HPalS) in terms of dependence on surface wettability and saliva concentration (<or=1%). Adsorbed amounts, kinetics, and elutability with buffer and sodium dodecyl sulphate (SDS) were determined. The low-molecular weight protein content of bulk HPalS was also investigated using two-dimensional gel electrophoresis, and this revealed the presence of a large group of proteins<100 kDa in size. Adsorption to pure (hydrophilic) and methylated (hydrophobized) silica surfaces revealed that the total adsorbed amounts were greater on hydrophobized silica. Below concentrations of 0.5 and 0.25% saliva, adsorption was concentration dependent on hydrophobized and hydrophilic surfaces, respectively. The initial adsorption (<or=30 min) was faster on hydrophobized surfaces. Addition of SDS removed more material than buffer rinsing on both surfaces. Analysis of the adsorption kinetics indicated that the presence of low-molecular weight proteins plays a role in adsorption from HPalS.
authors.affiliation: Prosthetic Dentistry, Faculty of Odontology, Malmö University, SE-205 06 Malmö, Sweden. ida.svendsen@od.mah.se
authors.forename: Ida E
authors.initials: IE
authors.lastname: Svendsen
authors.forename: Thomas
authors.initials: T
authors.lastname: Arnebrant
authors.forename: Liselott
authors.initials: L
authors.lastname: Lindh
mesh_terms: D000327:Adsorption; D007700:Kinetics; D008970:Molecular Weight; D010159:Palate; D011506:Proteins / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*
chemical_list: D011506:Proteins