High-performance liquid chromatographic determination of enalapril in human plasma by enzyme kinetic analytical method.
A high-performance liquid chromatographic method for indirect determination of enalapril in human plasma, was developed and validated. An exogenous angiotensin converting enzyme after drug inhibition was determined by reacting with hippuryl-histidyl-leucine to produce hippuric acid (HA) which was inversely proportional to the amount of enalaprilat in plasma. The HPLC was carried out on a Lichrosphere 60RP-select B, C18, 5 microm (125 mm x 4.0 mm i.d.) column at flow rate of 1.0 ml/min. The analysis time per injection was within 6.5 min. The lowest concentration of enalaprilat to be quantitated was 3.0 ng/ml with the acceptable accuracy and precision. This successfully developed method was practically and accurately used for pharmacokinetics and bioequivalent study of enalapril.
Thongnopnua
Phensri
P
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. tphensri@chula.ac.th
Poeaknapo
Chotima
C
D000328:Adult; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004656:Enalapril / Q000097:blood* / Q000493:pharmacokinetics; D015773:Enalaprilat / Q000097:blood; D006626:Hippurates / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000806:Angiotensin-Converting Enzyme Inhibitors; D006626:Hippurates; D012996:Solutions; D004656:Enalapril; D007703:Peptidyl-Dipeptidase A; D015773:Enalaprilat; C030514:hippuric acid