Application of SSB-like protein from Thermus aquaticus in multiplex PCR of human Y-STR markers identification.
Here, we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in multiplex PCR of human Y-STR markers identification. The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction of a number of primers available for annealing in PCR reaction.
Olszewski
Marcin
M
Department of Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-952 Gdańsk, Poland.
Rebała
Krzysztof
K
Szczerkowska
Zofia
Z
Kur
Józef
J
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*; D013824:Thermus
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins