Klk1 as one of the genes contributing to hypertension in Dahl salt-sensitive rat.
A genome-wide quantitative trait loci analysis for blood pressure was performed using 107 male F2 rats derived from Dahl salt-sensitive and Lewis rats. Blood pressure was assessed by telemetry, and >400 microsatellite markers were used for genotyping. Two major quantitative trait loci for blood pressure were identified at chromosome 1 and chromosome 10. The expression levels of 366 transcripts around the chromosome 1 quantitative trait loci were assessed by RT-PCR, and we found that the Klk1 (kallikrein 1) and Ngfg (nerve growth factor gamma) mRNA levels were significantly reduced in the kidneys of Dahl salt-sensitive rats compared with those in Lewis rats. The expression levels of kallikrein 1 protein were also suppressed in Dahl salt-sensitive rats compared with those in Lewis rats. Because the kallikrein-kinin system has been shown to be involved in renal function, including salt homeostasis, it is likely that the reduced expression of Klk1 contributes to salt-sensitive hypertension in Dahl salt-sensitive rats.
Iwai
Naoharu
N
Research Institute, National Cardiovascular Center, Suita, Osaka, Japan. iwai@ri.ncvc.go.jp
Yasui
Naomi
N
Naraba
Hiroaki
H
Tago
Naomi
N
Yamawaki
Hideyuki
H
Sumiya
Hiroshi
H
D000818:Animals; D001794:Blood Pressure; D002874:Chromosome Mapping; D008040:Genetic Linkage; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism; D008297:Male; D020932:Nerve Growth Factor / Q000235:genetics; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020303:Rats, Inbred Dahl / Q000235:genetics*; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020840:Tissue Kallikreins / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D020932:Nerve Growth Factor; D020840:Tissue Kallikreins