title: High-resolution functional proteomics by active-site peptide profiling.
abstract: Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.
authors.affiliation: ActivX Biosciences, 11025 North Torrey Pines Road, Suite 120, La Jolla, CA 92037, USA.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Okerberg
authors.forename: Jiangyue
authors.initials: J
authors.lastname: Wu
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
authors.forename: Babak
authors.initials: B
authors.lastname: Samii
authors.forename: Kelly
authors.initials: K
authors.lastname: Blackford
authors.forename: David T
authors.initials: DT
authors.lastname: Winn
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Shreder
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Burbaum
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Patricelli
mesh_terms: D000818:Animals; D001665:Binding Sites; D019075:Electrophoresis, Capillary; D051379:Mice; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012697:Serine Endopeptidases / Q000032:analysis / Q000737:chemistry
chemical_list: D010455:Peptides; C431704:Serhl protein, mouse; D012697:Serine Endopeptidases