Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays.
We have investigated the sensitivity of ex situ (analysis under air condition) and in situ (analysis under liquid condition) spectral SPR sensors, which were self-constructed with fiber optic spectrometers. The sensitivity of SPR sensors was analyzed in the wavelength range of 550-780 nm by the interactions of streptavidin and biotinylated IgG, and the sensitivity was dependent on the wavelength of measurements. The sensitivity of an ex situ SPR sensor operated at the long wavelength range from 712 nm was approximately 2.6 times higher than that at the short wavelength range from 571 nm. In addition, the sensitivity of an ex situ spectral SPR sensor was about twice as high as that of an in situ spectral SPR sensor for the same resonance wavelength range. This was interpreted in that the difference in sensitivity between two SPR sensors was significantly caused by the evanescent field intensity at the metal/dielectric interface. Thus, it was suggested that ex situ spectral SPR sensors operated at the long wavelength range are sensitive biosensors for the high-throughput analysis of protein interactions on protein arrays.
Yuk
Jong Seol
JS
Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University School of Medicine, Chunchon, Kangwon-Do 200-701, South Korea.
Jung
Jae-Wan
JW
Jung
Se-Hui
SH
Han
Jeong-A
JA
Kim
Young-Myeong
YM
Ha
Kwon-Soo
KS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D055100:Optical Fibers; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000032:analysis* / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D014159:Transducers
D007074:Immunoglobulin G; D019809:Streptavidin