Acute effect of gamma irradiation on gastric acid secretion and gastric mucosal integrity in the rat.
The effect of 3- or 6-Gray (Gy) whole-body gamma irradiation on basal and stimulated gastric acid secretion was studied in pylorus-ligated rats. Different groups of rats were irradiated with a single 3- or 6-Gy fraction and examined 7 days after irradiation. Exposure to 3-Gy fraction led to marked increase in basal (nonstimulated) gastric acid output in the 4-h pylorus-ligated rat (47.5% compared with unirradiated controls). After exposure to 6 Gy, only 18.2% increase in gastric acid output was noted compared with unirradiated controls. Under pentagastrin or histamine stimulation, gastric acid secretion in those irradiated with 3- or 6-Gy fraction was markedly reduced compared to that of unirradiated controls. Exposure to 3- or 6-Gy gamma irradiation intensified the degree of gastric mucosal injury evoked by indomethacin or 50% ethanol in a dose-dependent manner. It is concluded that in the pylorus-ligated rat model, lower doses of gamma irradiation increase basal gastric acid secretion and impair the gastric mucosal barrier with marked increase in its permeability to H+ following stimulation of acid secretion or exposure to barrier breakers. Exposure to irradiation is likely to result in failure of the parietal cell to respond to direct stimulation with histamine or pentagastrin.
Abdel Salam
Omar M E
OM
Department of Pharmacology, National Research Centre, Dokki, Cairo. omasalam@hotmail.com
Hadajat
Ihsan
I
Bayomy
Ayman Ragab
AR
El-Shinawy
Siham
S
Arbid
Mahmoud S
MS
D000818:Animals; D005720:Gamma Rays; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000528:radiation effects*; D006632:Histamine / Q000494:pharmacology; D010418:Pentagastrin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology; D014916:Whole-Body Irradiation
D006632:Histamine; D010418:Pentagastrin