title: Testosterone responsiveness of spleen and liver in female lymphotoxin beta receptor-deficient mice resistant to blood-stage malaria.
abstract: Disrupted signaling through lymphotoxin beta receptor (LTbetaR) results in severe defects of the spleen and even loss of all other secondary lymphoid tissues, making mice susceptible to diverse infectious agents. Surprisingly, however, we find that female LTbetaR-deficient mice are even more resistant to blood stages of Plasmodium chabaudi malaria than wild-type C57BL/6 mice. Higher resistance of LTbetaR-deficient mice correlates with an earlier onset of reticulocytosis, and the period of anemia is shorter. After surviving fulminant parasitemias of about 35%, mice develop long-lasting protective immunity against homologous rechallenge, with both spleen and liver acting as anti-malaria effectors. Testosterone suppresses resistance, i.e. all mice succumb to infections during or shortly after peak parasitemia. At peak parasitemia, testosterone does not essentially affect cellularity and apoptosis in the spleen, but aggravates liver pathology in terms of increased cell swelling, numbers of apoptotic and binucleated cells and reduced serum alkaline phosphatase levels, and conversely, reduces inflammatory lymphocytic infiltrates in the liver. In the spleen, hybridization of cDNA arrays identified only a few testosterone-induced changes in gene expression, in particular upregulation of INFgamma and IFN-regulated genes. By contrast, a much larger number of testosterone-affectable genes was observed in the liver, including genes involved in regulation of the extracellular matrix, in chemokine and cytokine signaling, and in cell cycle control. Collectively, our data suggest that testosterone dysregulates the inflammatory response in spleen and liver during their differentiation to anti-malaria effectors in malaria-resistant female LTbetaR-deficient mice, thus contributing to the testosterone-induced lethal outcome of malaria.
authors.affiliation: Division of Molecular Parasitology and Biological and Medical Research Center, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Wunderlich
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Dkhil
authors.forename: Liv I
authors.initials: LI
authors.lastname: Mehnert
authors.forename: Juliane V
authors.initials: JV
authors.lastname: Braun
authors.forename: Manal
authors.initials: M
authors.lastname: El-Khadragy
authors.forename: Elena
authors.initials: E
authors.lastname: Borsch
authors.forename: Derik
authors.initials: D
authors.lastname: Hermsen
authors.forename: W Peter M
authors.initials: WP
authors.lastname: Benten
authors.forename: Klaus
authors.initials: K
authors.lastname: Pfeffer
authors.forename: Horst
authors.initials: H
authors.lastname: Mossmann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Krücken
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D008099:Liver / Q000187:drug effects*; D053259:Lymphotoxin beta Receptor; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018512:Parasitemia / Q000276:immunology; D016788:Plasmodium chabaudi; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000187:drug effects*; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C505699:Ltbr protein, mouse; D053259:Lymphotoxin beta Receptor; D018124:Receptors, Tumor Necrosis Factor; D013739:Testosterone