Clonal analyses of HIV quasispecies in patients harbouring plasma genotype with K65R mutation associated with thymidine analogue mutations or L74V substitution.
We analysed the quasispecies at a clonal level in patients whose plasma genotypic test harboured K65R with L74V or thymidine analogue mutations (TAM). We showed that the K65R and TAM such as M41L, D67N, T215Y/D, L210W and K219E can be borne by the same virus. We found no clone bearing both K65R and L74V substitutions. Moreover, the S68G and V75I mutations are not necessarily linked with K65R, and could thus have their own resistance effect.
Wirden
Marc
M
Department of Virology, Pitié-Salpêtrière Hospital, Paris, France.
Malet
Isabelle
I
Derache
Anne
A
Marcelin
Anne Genevieve
AG
Roquebert
Benedicte
B
Simon
Anne
A
Kirstetter
Myriam
M
Joubert
Laurence Morand
LM
Katlama
Christine
C
Calvez
Vincent
V
D019943:Amino Acid Substitution / Q000235:genetics; D002999:Clone Cells; D005838:Genotype; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics*; D013936:Thymidine / Q000031:analogs & derivatives / Q000235:genetics*
D013936:Thymidine