title: Modulation of macrophage functions by sheeppox virus provides clues to understand interaction of the virus with host immune system.
abstract: BACKGROUND
  Poxviruses encode a range of immunomodulatory genes to subvert or evade the challenges posed by the innate and adaptive immune responses. However, the inactivated poxviruses possessed immunostimulating capacity and were used as a prophylactic or metaphylactic application that efficiently reduced susceptibility to infectious diseases in different species. This fact is intensively studied in different genera of poxviruses. However, little is known about the basic mechanisms adopted by sheeppox virus (SPPV). SPPV causes an acute disease of sheep that recently, has been observed to reinfect its host in spite of vaccination.
  
  
  RESULTS
  By injecting inactivated or attenuated sheeppox virus SPPV vaccine in adult male Swiss mice, SPPV was found to reduce macrophages' functions in a local event that occurs at the site of application 12 h after vaccine administration as indicated by increased level of IL-10 and decreased level of SOD from cultured peritoneal macrophages. In contrast increased levels of IL-12, and SOD activity from cultured splenic macrophages, lymphocyte response to PHA-P, and in-vivo response to T-dependant Ag were detected. These effects were observed in both attenuated and inactivated SPPV, but more prominent in attenuated one.
  
  
  CONCLUSIONS
  The results of this study help to elucidate, the phenomenon of existence natural SPPV infections in sheep instead of vaccination and the basic mechanisms responsible for the immunostimulating capacity of sheeppox virus. Locally, SPPV shows evidence for an immune escape mechanism that alleviates the host's immune response. Later and systemically, the virus protects the host from any fatal consequences of the immune system suppression.
authors.affiliation: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Beni-Suef 62511, Egypt. elsaad1@yahoo.com
authors.forename: Abdel-Aziz S
authors.initials: AA
authors.lastname: Abu-El-Saad
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Abdel-Moneim
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D010835:Phytohemagglutinins; D011213:Poxviridae Infections / Q000276:immunology*; D013154:Spleen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology
chemical_list: D010835:Phytohemagglutinins; C028784:phytohemagglutinin-P; D016753:Interleukin-10; D018664:Interleukin-12; D013482:Superoxide Dismutase