title: Leupaxin binds to PEST domain tyrosine phosphatase PEP.
abstract: PEST domain tyrosine phosphatase (PEP) is an intracellular protein tyrosine phosphatase and characterized by PEST motifs and proline-rich domains in the carboxyl terminal half. PEP is primarily expressed in hematopoietic cells, and together with PEP-binding Csk, may act as a negative regulator of antigen receptor signaling in lymphocytes. Here, we show the binding capability of PEP for leupaxin, which is preferentially expressed in hematopoietic cells and a comparatively new member of the paxillin family characterized by two protein-protein interaction modules, LIM domains and LD motifs. These results suggested that leupaxin might participate in the regulation of the signaling cascade through the binding to PEP in lymphocytes.
authors.affiliation: Department of Life Science and Frontier Project "Life's Adaptation Strategies to Environmental Changes," Rikkyo (St Paul's) University, Nishi-Ikebukuro, Toshima-ku, Tokyo, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Watanabe
authors.forename: Natsuko
authors.initials: N
authors.lastname: Amano
authors.forename: Hajime
authors.initials: H
authors.lastname: Ishizuka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Mashima
mesh_terms: D020816:Amino Acid Motifs; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D015815:Cell Adhesion Molecules; C112251:LPXN protein, human; D010750:Phosphoproteins; C516738:PTPN12 protein, human; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12; D017027:Protein Tyrosine Phosphatases