The inhibitor protein of the F1F0-ATP synthase is associated to the external surface of endothelial cells.
The ATPase inhibitor protein (IP) of mitochondria was detected in the plasma membrane of living endothelial cells by flow cytometry, competition assays, and confocal microscopy of cells exposed to IP antibodies. The plasma membranes of endothelial cells also possess beta-subunits of the mitochondrial ATPase. Plasma membranes have the capacity to bind exogenous IP. TNF-alpha decreases the level of beta-subunits and increases the amount of IP, indicating that the ratio of IP to beta-subunit exhibits significant variations. Therefore, it is probable that the function of IP in the plasma membrane of endothelial cells is not limited to regulation of catalysis.
Cortés-Hernández
Paulina
P
Instituto Nacional de Cardiología Ignacio Chávez, Mexico.
Domínguez-Ramírez
Lenin
L
Estrada-Bernal
Adriana
A
Montes-Sánchez
Delina G
DG
Zentella-Dehesa
Alejandro
A
de Gómez-Puyou
Marietta Tuena
MT
Gómez-Puyou
Armando
A
García
José J
JJ
D000906:Antibodies / Q000276:immunology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D025261:Mitochondrial Proton-Translocating ATPases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D000097795:ATPase Inhibitory Protein
D000906:Antibodies; D025261:Mitochondrial Proton-Translocating ATPases; D021122:Protein Subunits; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D000097795:ATPase Inhibitory Protein; C474451:F1F0-ATP synthase