title: Synthesis and circular dichroism of tetraarylporphyrin-oligonucleotide conjugates.
abstract: The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3' end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28-40 A), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400-450 nm). The porphyrin chromophores act as sensitive probes of geometrical changes in the DNA backbone and sensitively reflect the double-strand to single-strand transition. This study demonstrates the possibility of using exciton-coupled porphyrin CDs for conformational studies of DNA.
authors.affiliation: Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027, USA.
authors.forename: Milan
authors.initials: M
authors.lastname: Balaz
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Holmes
authors.forename: Michele
authors.initials: M
authors.lastname: Benedetti
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Rodriguez
authors.forename: Nina
authors.initials: N
authors.lastname: Berova
authors.forename: Koji
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Gloria
authors.initials: G
authors.lastname: Proni
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D011166:Porphyrins; D004247:DNA