[Determination of the content of geniposide in xinxue granules by high performance liquid chromatography].
A high performance liquid chromatographic method has been developed for the determination of geniposide in Xinxue granules. Geniposide was extracted by ultrasonic extraction for 30 min. The chromatographic separation was carried out on a Diamonsil C18 column (200 mm x 4. 6 mm i.d., 5 microm) with a mobile phase consisting of an acetonitrile-water (15:85, v/v) mixture. The detection wavelength was set at 238 nm. The calibration curve was linear in the ranged of 25 - 400 mg/L for geniposide. The average recovery of geniposide was 101.2% with a relative standard deviation (RSD) of 1.6% and contents of geniposide in the granules ranged from 0.841 to 0.923 mg/g. This method is simple, reliable and suitable for quality control of Xinxue granules.
Ran
Guimei
G
Shenyang Pharmaceutical University, Shenyang 110016, China.
Yang
Ling
L
Zhang
Caihua
C
Guo
Xingjie
X
D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D039823:Iridoids / Q000032:analysis*; D011714:Pyrans / Q000032:analysis*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D039823:Iridoids; D011714:Pyrans; C007835:geniposide