Expression of cell cycle associated proteins cyclin A, CDK-2, p27kip1 and p53 in equine sarcoids.
Equine sarcoids are benign fibroblastic skin tumours affecting equids worldwide. Whilst the pathogenesis is not entirely understood, infection with Bovine Papillomavirus (BPV) types 1 and 2 has been implicated as a major factor in the disease process, however the mechanism by which BPV infection contributes to sarcoid pathology is not clear. In this study, we show that the majority of sarcoids express the BPV-1 major transforming gene E6. Further, we demonstrate that sarcoid lesions are not associated with high levels of cellular proliferation as assessed by Ki67 expression or with expression of cell cycle regulators CDK-2, cyclin A and p27kip1. Our analysis of p53 shows that a subset of sarcoids are associated with abnormal cytoplasmic and nuclear expression of p53 and that the transactivation function of p53 is compromised in cells with cytoplasmic p53.
Nixon
Colin
C
Division of Pathological Sciences, Institute of Comparative Medicine, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 1QH, Scotland, UK.
Chambers
George
G
Ellsmore
Victoria
V
Campo
M Saveria
MS
Burr
Paul
P
Argyle
David J
DJ
Reid
Stuart W J
SW
Nasir
Lubna
L
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000472:pathogenicity; D042846:CDC2-CDC28 Kinases / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism*; D051357:Cyclin-Dependent Kinase 2; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D015967:Gene Expression Regulation, Viral; D006734:Horse Diseases / Q000378:metabolism / Q000821:virology; D006736:Horses; D019394:Ki-67 Antigen / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000662:veterinary / Q000821:virology; D012871:Skin Diseases / Q000378:metabolism / Q000662:veterinary / Q000821:virology; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D019925:Cyclin A; D004279:DNA, Viral; D019394:Ki-67 Antigen; D009856:Oncogene Proteins, Viral; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2