Identification of the alternative promoters of the KChIP4 subfamily.
The subfamily of voltage-dependent potassium (Kv) channel interacting protein 4 (KChIP4) is made up of the auxiliary interacting protein of voltage-dependent potassium channels. In this study, the structure of four splicing variants of the human KChIP4 gene was analyzed. Three of the four isoforms of the KChIP4 gene, KChIP4.1, KChIP4.2 and KChIP4.4, were amplified from mouse and human fetal brain tissues by reverse transcription-polymerase chain reaction and then identified. Based on the bioinformatics analysis of the genomic sequences of the gene, we cloned and characterized two promoter fragments from the KChIP4 gene. One was a 325 bp fragment upstream of the 5' end of the KChIP4.1 mRNA sequence and the other was an 818 bp fragment located immediately at the 5' end of the KChIP4.4 variant. Both of them can initiate the transcription of the reporter gene in HT1080 cells and Sprague-Dawley (SD) rat fetal brain neurons, and they contain CG islands, except typical TATA boxes and CAAT boxes. This shows that the KChIP4 gene expression is regulated by an alternative promoter.
Deng
Xiao-Yun
XY
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Cai
Fang
F
Xia
Kun
K
Pan
Qian
Q
Long
Zhi-Gao
ZG
Wu
Ling-Qian
LQ
Liang
De-Sheng
DS
Dai
He-Ping
HP
Zhang
Zhuo-Hua
ZH
Xia
Jia-Hui
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000235:genetics* / Q000378:metabolism*; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D051605:Kv Channel-Interacting Proteins; D051379:Mice; D008969:Molecular Sequence Data; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
C496753:KCNIP4 protein, human; D051605:Kv Channel-Interacting Proteins; D024642:Potassium Channels, Voltage-Gated; D020033:Protein Isoforms