[Laboratory diagnostics of urogenital clamidiosis].
Laboratory diagnostic tools of urogenital clamidiosis--PCR, ELISA (IgG and IgM) and direct immunofluorescence (DIF)--were comparatively analyzed. The positive PCR result was checked by another PCR test with a different primer; 5 false positive responses were registered (specificity 99.6%). As against PCR, the sensitivity of ELISA made 53%, its specificity -75.5%, the diagnostic value of positive result -58%, the diagnostic value of negative result -71.6% and the diagnostics accuracy -66.7%. The respective DIF parameters were as follows: 36%, 90%, 81.5%, 54.2% and 60.9%. A high rate of detection (above 90%) of the conditionally pathogenic microflora associated with Chlamydia trachomatis (above 110 microbe cells/ml) was pointed out. Hardnerelli and ureaplasms were more often found in female smears, staphylococci and enterococci--in male sperm. It is underlined as important to hold complex examinations for Chlamidia (PCR, ELISA and DIC as an additional test) combined with bacteriological quantification of the conditionally pathogenic microflora and determination of its resistance to antibiotics.
Churakov
A A
AA
Kulichenko
A N
AN
Kzakova
E S
ES
Serebrianik
N E
NE
Suvorov
A P
AP
Kutyrev
V V
VV
Glybochko
P V
PV
D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases; D016133:Polymerase Chain Reaction