Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome.
The double-stranded DNA genome of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) was sequenced and analysed in the context of other group I nucleopolyhedroviruses (NPVs). The genome consists of 129,593 bp with a G + C content of 50.1 mol%. A total of 146 open reading frames (ORFs) of greater than 150 bp, and with no or minimal overlap were identified. In addition, five homologous regions were identified containing 7-10 repeats of a 36 bp imperfect palindromic core. Comparison with other completely sequenced baculovirus genomes revealed that 139 of the CfMNPV ORFs have homologues in at least one other baculovirus and seven ORFs are unique to CfMNPV. Of the 117 CfMNPV ORFs common to all group I NPVs, 12 are exclusive to group I NPVs. Overall, CfMNPV is most similar to Orgyia pseudotsugata MNPV based on gene content, arrangement and overall amino acid identity. Unlike other group I baculoviruses, however, CfMNPV encodes a viral enhancing factor (vef) and has two copies of p26.
de Jong
Jondavid G
JG
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Lauzon
Hilary A M
HAM
Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada, P6A 2E5.
Dominy
Cliff
C
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Poloumienko
Arkadi
A
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Carstens
Eric B
EB
Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada, K7L 3N6.
Arif
Basil M
BM
Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada, P6A 2E5.
Krell
Peter J
PJ
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins