title: Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture.
abstract: Applying a newly developed user-interactive optical trapping system, we controllably surrounded individual cells of one yeast species, Hanseniaspora uvarum, with viable cells of another yeast species, Saccharomyces cerevisiae, thus creating a confinement of the former. Growth of surrounded and non-surrounded H. uvarum cells was followed under a microscope by determining their generation time. The average generation time of surrounded H. uvarum cells was 15% higher than that of non-surrounded cells, thereby showing that the confinement imposed by viable S. cerevisiae cells on H. uvarum inhibits growth of the latter. This study is the first to demonstrate that confinement is a determinant of growth in a microbial ecosystem.
authors.affiliation: Department of Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
authors.forename: Nils
authors.initials: N
authors.lastname: Arneborg
authors.forename: Henrik
authors.initials: H
authors.lastname: Siegumfeldt
authors.forename: Grith H
authors.initials: GH
authors.lastname: Andersen
authors.forename: Peter
authors.initials: P
authors.lastname: Nissen
authors.forename: Vincent R
authors.initials: VR
authors.lastname: Daria
authors.forename: Peter John
authors.initials: PJ
authors.lastname: Rodrigo
authors.forename: Jesper
authors.initials: J
authors.lastname: Glückstad
mesh_terms: D003470:Culture Media; D017753:Ecosystem; D018869:Heat-Shock Response; D007091:Image Processing, Computer-Assisted; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D004718:Saccharomycetales / Q000254:growth & development*
chemical_list: D003470:Culture Media