Pseudorabies virus propagated in rabbit kidney-derived RK13 cells is neutralized by natural IgM antibodies in normal swine serum which specifically lyse host cells.
Pseudorabies virus (PRV) propagated in rabbit kidney-derived RK-13 cells (PRV-RK) was neutralized by serum obtained from specific pathogen-free pigs through the activation of complement. The virus-neutralizing activity of swine serum was lost after treatment with ethylene glycol-bis-aminoethylether-N,N,N',N'-tetraacetic acid (EGTA) or ethylenediaminetetraacetic acid (EDTA). Anti-C1q and anti-IgM antibodies also inhibited virus-neutralizing activity. Though IgG-depleted swine serum neutralized PRV, IgM and IgG-free swine serum lost virus-neutralizing activity. Pre-incubation of swine serum with RK-13 cells, but not with swine kidney-derived CPK cells, at 4 degrees C eliminated the virus-neutralizing activity to PRV-RK. Results indicated that swine serum contained natural IgM against an antigen(s) on the RK-13 cell surface and that this surface antigen was integrated into the PRV envelope during the budding process. Thus the natural IgM in swine serum reacted with the RK-13 antigen on the viral envelope, activated the complement cascade and neutralized the PRV-RK.
Hayashi
Sunao
S
Department of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Takashima
Yasuhiro
Y
Otsuka
Haruki
H
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002478:Cells, Cultured; D003167:Complement Activation / Q000187:drug effects / Q000276:immunology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007106:Immune Sera / Q000187:drug effects / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D009500:Neutralization Tests; D011817:Rabbits; D013552:Swine / Q000097:blood* / Q000276:immunology
D000914:Antibodies, Viral; D000954:Antigens, Surface; D007106:Immune Sera; D007075:Immunoglobulin M; D004533:Egtazic Acid; D004492:Edetic Acid