GST fusion vector with caspase-6 cleavage site for removal of fusion tag during column purification.
Purbey
Prabhat Kumar
PK
National Centre for Cell Science, Ganeshkhind, Pune, India.
Jayakumar
P Cyril
PC
Deepalakshmi
P D
PD
Patole
Milind S
MS
Galande
Sanjeev
S
D001665:Binding Sites; D053178:Caspase 6; D020169:Caspases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*
D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D053178:Caspase 6; D020169:Caspases