Interferon-gamma associated cytokines and chemokines produced by spleen cells from Brucella-immune mice.
It is known that interferon (IFN)-gamma plays a critical role in protection against brucellosis. In this study we have investigated several cytokines and chemokines that are associated with IFN-gamma for potential in vitro correlates of protection. We cultured spleen cells in vitro from mice immunized orally with a live, attenuated Brucella melitensis vaccine candidate (WR201) and stimulated these cells with a lysate of B. melitensis. Differential gene expression of several cytokines and chemokines in stimulated spleen cells was analysed by real-time PCR, and secreted proteins were determined by ELISA. Immunized mice produced higher levels of both protein and gene transcripts for IFN-gamma, interleukin (IL)-2, IL-18 and MIP1-alpha. Immunized mice also had elevated gene expression levels for IL12-p40, IL23-p19, IP-10, MIG and MCP-1 when compared to normal mice. In this study we have identified new cytokines and chemokines as potential immune correlates in responses to protection in Brucella-vaccinated mice.
Paranavitana
Chrysanthi
C
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Bldg 503, Room 3E10, Robert Grant Avenue, Silver Spring, MD 20910, USA. chrysanthi.paranavitana@na.amedd.army.mil
Zelazowska
Elzbieta
E
Izadjoo
Mina
M
Hoover
David
D
D000818:Animals; D002004:Brucella Vaccine / Q000276:immunology; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control; D018925:Chemokines / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007371:Interferon-gamma / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012016:Reference Values; D013154:Spleen / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
D002004:Brucella Vaccine; D018925:Chemokines; D016207:Cytokines; D007371:Interferon-gamma