title: Reproduction and cytogenetic characterization of interspecific hybrids derived from crosses between Brassica carinata and B. rapa.
abstract: The tri-genomic hybrid (ABC, 2n=27) between Brassica carinata (BBCC, 2n=34) and B. rapa (AA, 2n=20) is a unique material for studying genome relationships among Brassica species and a valuable bridge for transferring desirable characteristics from one species to the other within the genus Brassica. The crossability between B. carinata and B. rapa was varied with the cultivar of B. rapa. Hybrid pollen mother cells (PMCs), confirmed by morphological observation and molecular marker assay, could be grouped into 20 classes on the basis of chromosome pairing configurations. More than 30% of the PMCs had nine or more bivalents. Genomic in situ hybridization confirmed that two of the bivalents most likely belonged to the B genome. Nearly one-half of the PMCs had trivalents (0-2) and quadrivalents (0-2), which revealed partial homology among the A, B, and C genomes and suggested that there is a good possibility to transfer genes by means of recombination among the three genomes. The advantages of using the tri-genomic hybrids as bridge material for breeding new types of B. napus are discussed.
authors.affiliation: National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: M T
authors.initials: MT
authors.lastname: Li
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Li
authors.forename: C Y
authors.initials: CY
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Qian
authors.forename: J L
authors.initials: JL
authors.lastname: Meng
mesh_terms: D001937:Brassica / Q000235:genetics* / Q000502:physiology; D001947:Breeding / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics*; D016000:Cluster Analysis; D020732:Cytogenetic Analysis; D006824:Hybridization, Genetic; D017403:In Situ Hybridization; D021141:Nucleic Acid Amplification Techniques; D011058:Pollen / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012098:Reproduction / Q000502:physiology