Effects of purified herbal extract of Salvia miltiorrhiza on ischemic rat myocardium after acute myocardial infarction.
In the current study, we compared purified Salvia miltiorrhiza extract (PSME) with Angiotensin-converting enzyme inhibitor, Ramipril, in in vitro experiments and also in vivo using animal model of myocardial infarction. PSME was found to have a significantly higher trolox equivalent antioxidant capacity which indicated a great capacity for scavenging free radicals. PSME could also prevent pyrogallo red bleaching and DNA damage. After 2 weeks treatment with PSME or Ramipril, survival rates of rats with experimental myocardial infarction were marginally increased (68.2% and 71.4%) compared with saline (61.5%). The ratios of infarct size to left ventricular size in both PSME-and Ramipril-treated rats were significantly less than that in the saline-treated group. Activity of cardiac antioxidant enzyme superoxide dismutase (SOD) was significant higher while level of Thiobarbituric acid-reactive substances (TBARs) was lower in the PSME treated group. Purified and standardized Chinese herb could provide an alternative regimen for the prevention of ischemic heart disease.
Sun
Jian
J
Department of Pharmacology, National University of Singapore, Singapore.
Huang
Shan Hong
SH
Tan
Benny K-H
BK
Whiteman
Matt
M
Zhu
Yi Chun
YC
Wu
Ya Jun
YJ
Ng
Yeekong
Y
Duan
Wei
W
Zhu
Yi Zhun
YZ
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D052160:Benzothiazoles; D004249:DNA Damage / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D009203:Myocardial Infarction / Q000150:complications*; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000209:etiology; D008517:Phytotherapy; D011748:Pyrogallol / Q000031:analogs & derivatives*; D017257:Ramipril / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013053:Spectrophotometry; D013451:Sulfonic Acids; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects
D000975:Antioxidants; D052160:Benzothiazoles; D004365:Drugs, Chinese Herbal; D013451:Sulfonic Acids; D017392:Thiobarbituric Acid Reactive Substances; D011748:Pyrogallol; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C058731:pyrogallol sulfonphthalein; D013482:Superoxide Dismutase; D017257:Ramipril