High-resolution functional proteomics by active-site peptide profiling.
Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.
Okerberg
Eric S
ES
ActivX Biosciences, 11025 North Torrey Pines Road, Suite 120, La Jolla, CA 92037, USA.
Wu
Jiangyue
J
Zhang
Baohong
B
Samii
Babak
B
Blackford
Kelly
K
Winn
David T
DT
Shreder
Kevin R
KR
Burbaum
Jonathan J
JJ
Patricelli
Matthew P
MP
D000818:Animals; D001665:Binding Sites; D019075:Electrophoresis, Capillary; D051379:Mice; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012697:Serine Endopeptidases / Q000032:analysis / Q000737:chemistry
D010455:Peptides; C431704:Serhl protein, mouse; D012697:Serine Endopeptidases