Preparative separation and purification of deoxyschisandrin and gamma-schisandrin from Schisandra chinensis (Turcz.) Baill by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of deoxyschisandrin and gamma-schisandrin from the crude petroleum ether extracts of Schisandra chinensis (Turcz.) Baill. The optimum solvent system composed of n-hexane-methanol-water (35:30:3, v/v) led to the successful preparation of deoxyschisandrin and gamma-schisandrin. The analysis of HPLC for each peak fraction of preparative HSCCC showed that the purity of deoxyschisandrin (8 mg) was over 98% and gamma-schisandrin (12 mg) was over 96% from 100 mg of the crude petroleum ether extracts in one-step separation.
Huang
Tianhui
T
Institute of Chemical and Pharmaceutical, East China University of Science and Technology, 897 #, 130 Meilong Road, Shanghai 200237, China. hth169@yahoo.com.cn
Shen
Pingniang
P
Shen
Yongjia
Y
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D034242:Cyclooctanes / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D017705:Lignans / Q000302:isolation & purification*; D011083:Polycyclic Compounds / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*; D012997:Solvents
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D012997:Solvents; C015499:schizandrin B; C034734:schizandrin A