title: Structure of a liganded type 2 non-specific lipid-transfer protein from wheat and the molecular basis of lipid binding.
abstract: In plants, a family of ubiquitous proteins named non-specific lipid-transfer proteins (ns-LTPs) facilitates the transfer of fatty acids, phospholipids and steroids between membranes. Recent data suggest that these secreted proteins play a key role in the formation of cuticular wax layers and in defence mechanisms against pathogens. In this study, X-ray crystallography has been used to examine the structural details of the interaction between a wheat type 2 ns-LTP and a lipid, L-alpha-palmitoyl-phosphatidyl glycerol. This crystal structure was solved ab initio at 1.12 A resolution by direct methods. The typical alpha-helical bundle fold of this protein is maintained by four disulfide bridges and delineates two hydrophobic cavities. The inner surface of the main cavity is lined by non-polar residues that provide a hydrophobic environment for the palmitoyl moiety of the lipid. The head-group region of this lipid protrudes from the surface and makes several polar interactions with a conserved patch of basic residues at the entrance of the pocket. The alkyl chain of a second lipid is bound within an adjacent smaller cavity. The structure shows that binding of the lipid tails to the protein involves extensive hydrophobic interactions.
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048-INSERM U554, 29 Rue de Navacelles, 34090 Montpellier, France.
authors.forename: François
authors.initials: F
authors.lastname: Hoh
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pons
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Gautier
authors.forename: Frédéric
authors.initials: F
authors.lastname: de Lamotte
authors.forename: Christian
authors.initials: C
authors.lastname: Dumas
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D019281:Dimerization; D057927:Hydrophobic and Hydrophilic Interactions; D050356:Lipid Metabolism; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D016415:Sequence Alignment; D014908:Triticum / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; C072087:sterol carrier proteins