title: Minute virus of mice small non-structural protein NS2 localizes within, but is not required for the formation of, Smn-associated autonomous parvovirus-associated replication bodies.
abstract: The non-structural proteins NS1 and NS2 of the parvovirus minute virus of mice (MVM) are required for efficient virus replication. It has previously been shown that NS1 and NS2 interact and colocalize with the survival motor neuron (Smn) gene product in novel nuclear structures that are formed late in infection, termed Smn-associated APAR (autonomous parvovirus-associated replication) bodies (SAABs). It is not clear what molecular viral intermediate(s) contribute to SAAB formation. The current results address the role of NS2 in SAAB formation. In highly synchronized wild-type MVM infection of murine A9(2L) cells, NS2 colocalizes with Smn and other SAAB constituents. An MVM mutant that does not produce NS2 still generates SAABS, albeit with a temporal delay. The lag in SAAB formation seen in the absence of NS2 is probably related to the temporal delay in virus replication, suggesting that, whilst NS2 is required for efficient viral infection, it is dispensable for SAAB formation.
authors.affiliation: Department of Veterinary Pathobiology, Life Sciences Center 471G, University of Missouri, Columbia, MO 65211-7310, USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Young
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Newman
authors.affiliation: Novozymes, 1AS.31, Novo Alle, DK-2880 Bagsværd, Denmark.
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Jensen
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Burger
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Pintel
authors.affiliation: Department of Veterinary Pathobiology, Life Sciences Center 471G, University of Missouri, Columbia, MO 65211-7310, USA.
authors.forename: Christian L
authors.initials: CL
authors.lastname: Lorson
mesh_terms: D000818:Animals; D001324:Autoantigens; D002460:Cell Line; D020541:Coiled Bodies / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D051379:Mice; D008915:Minute Virus of Mice / Q000472:pathogenicity / Q000502:physiology*; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication; D055517:snRNP Core Proteins
chemical_list: D001324:Autoantigens; D017411:Ribonucleoproteins, Small Nuclear; D017361:Viral Nonstructural Proteins; D055517:snRNP Core Proteins