[The effects of HSV-tk suicide gene and wild-type p53 gene on pleomorphic adenoma cells of salivary gland].
OBJECTIVE
To study the therapeutic effects of combined gene therapy of wild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.


METHODS
Wild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The efficiency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.


RESULTS
The proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, when wt-p53 gene combined with HSV-tk/GCV system, the killing effects was significantly stronger (P < 0.05) and the cell survival rate decreased to 20%.


CONCLUSIONS
Combining p53 gene with HSV-tk/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.
Wang
Xu
X
Dept. of Oral Pathology, School of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
Wang
Jie
J
Dong
Fu-sheng
FS
Dong
Yu-ying
YY
Hou
Ya-li
YL
D008949:Adenoma, Pleomorphic; D000998:Antiviral Agents; D045744:Cell Line, Tumor; D002470:Cell Survival; D015774:Ganciclovir; D016158:Genes, p53; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D012469:Salivary Glands; D018139:Simplexvirus; D013937:Thymidine Kinase; D014162:Transfection
D000998:Antiviral Agents; D013937:Thymidine Kinase; D015774:Ganciclovir