title: The inhibitor protein of the F1F0-ATP synthase is associated to the external surface of endothelial cells.
abstract: The ATPase inhibitor protein (IP) of mitochondria was detected in the plasma membrane of living endothelial cells by flow cytometry, competition assays, and confocal microscopy of cells exposed to IP antibodies. The plasma membranes of endothelial cells also possess beta-subunits of the mitochondrial ATPase. Plasma membranes have the capacity to bind exogenous IP. TNF-alpha decreases the level of beta-subunits and increases the amount of IP, indicating that the ratio of IP to beta-subunit exhibits significant variations. Therefore, it is probable that the function of IP in the plasma membrane of endothelial cells is not limited to regulation of catalysis.
authors.affiliation: Instituto Nacional de Cardiología Ignacio Chávez, Mexico.
authors.forename: Paulina
authors.initials: P
authors.lastname: Cortés-Hernández
authors.forename: Lenin
authors.initials: L
authors.lastname: Domínguez-Ramírez
authors.forename: Adriana
authors.initials: A
authors.lastname: Estrada-Bernal
authors.forename: Delina G
authors.initials: DG
authors.lastname: Montes-Sánchez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zentella-Dehesa
authors.forename: Marietta Tuena
authors.initials: MT
authors.lastname: de Gómez-Puyou
authors.forename: Armando
authors.initials: A
authors.lastname: Gómez-Puyou
authors.forename: José J
authors.initials: JJ
authors.lastname: García
mesh_terms: D000906:Antibodies / Q000276:immunology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D025261:Mitochondrial Proton-Translocating ATPases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D000097795:ATPase Inhibitory Protein
chemical_list: D000906:Antibodies; D025261:Mitochondrial Proton-Translocating ATPases; D021122:Protein Subunits; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D000097795:ATPase Inhibitory Protein; C474451:F1F0-ATP synthase