Gene expression profiles reveal that DCN, DIO1, and DIO2 are underexpressed in benign and malignant thyroid tumors.
To investigate the molecular events involved in the pathogenesis and/or progression of thyroid tumors, we compared the gene expression profiles of three thyroid carcinoma cell lines, which represent major tumor subtypes of thyroid cancer and normal thyroid tissue. Using cDNA array methodology, we investigated the expression of 1807 open reading frame expressed sequence tags (ORESTES), selected from head and neck tumor libraries generated through the Brazilian Human Cancer Project-LICR/FAPESP. We found that 505 transcripts were differentially expressed in the thyroid carcinoma cell lines. Using a more stringent criterion, transcripts underexpressed or overexpressed more than fivefold in 1 of 3 or 3 of 3 carcinoma cell lines, a list of 55 ESTs were detected. Five candidate genes were further validated by quantitative polymerase chain reaction (qPCR) in an independent set of 52 thyroid tumors and 22 matched normal thyroid tissues. DCN was found underexpressed in a high percentage of the follicular thyroid adenomas, follicular thyroid carcinomas, and follicular variant of papillary thyroid carcinomas. DIO1 and DIO2 were underexpressed in nearly all papillary thyroid carcinomas. These genes not only could help to better define a tumor signature for thyroid tumors, but may, in part, also become useful as potential targets for thyroid tumor treatment.
Arnaldi
L A T
LA
Laboratory of Molecular Endocrinology, Division of Endocrinology, Department of Medicine, Federal University of São Paulo, Rua Pedro de Toledo 781-12 andar 04039-032, São Paulo, Brazil.
Borra
R C
RC
Maciel
R M B
RM
Cerutti
J M
JM
D018263:Adenocarcinoma, Follicular / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D017931:DNA Primers; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000235:genetics*; D013961:Thyroid Gland / Q000502:physiology*; D013964:Thyroid Neoplasms / Q000235:genetics*
C546878:DCN protein, human; D017931:DNA Primers; D058575:Decorin; D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; D011509:Proteoglycans; D007453:Iodide Peroxidase