Transcription factors as targets of the anti-inflammatory treatment. A cell culture study with extracts from some Mediterranean diet plants.
During the inflammatory response at least 2 transcription factors, NF-kappaB and AP-1, are involved in the altered profile of gene expression. We used human hepatoma (HepG2) and human umbilical vein endothelial cells (HUVEC) as a model system: NF-kappaB and AP-1 were activated by the proinflammatory cytokine IL-1 in the absence or presence of 21 selected plant extracts and the effect was evaluated by the electrophoretic mobility shift assay (EMSA). In both types of cells activation of NF-kappaB by IL-1 was significantly inhibited by extracts from Scandix australis and Artemisia alba, whereas extracts from Amaranthus sp., Eryngium campestre, Thymus pulegioides and Reichardia picroides elicited cell-type dependent response. The IL-1-induced AP-1 activation was diminished by extracts from Scandix australis, Amaranthus sp. and Artemisia alba more potently in HUVEC, while extracts from Urospermum picroides and Scandix pecten-veneris in HepG2 cells. Inhibitory activities of plant extracts towards cytokine activated NF-kappaB and AP-1 depend to some extent on the order of addition of IL-1 and plant extract to the cell culture, but the mechanism of action of extract components is not clear: although plant polyphenols may participate they are unlikely to be the only mediators, and MAP kinases were found generally not involved in down-regulation of transcription factors activity by plant extracts.
Stalińska
K
K
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland.
Guzdek
A
A
Rokicki
M
M
Koj
A
A
D000111:Acetylcysteine / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology; D038441:Diet, Mediterranean; D024202:Electrophoretic Mobility Shift Assay; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D050791:STAT Transcription Factors / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D050791:STAT Transcription Factors; D012717:Sesquiterpenes; D018808:Transcription Factor AP-1; C002669:parthenolide; D011794:Quercetin; D020928:Mitogen-Activated Protein Kinases; D003474:Curcumin; D000111:Acetylcysteine