title: Differential gene expression profiling of human umbilical cord blood-derived mesenchymal stem cells by DNA microarray.
abstract: Mesenchymal stem cells (MSCs) retain both self-renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high-density oligonucleotide micro-arrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50-fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton-associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray-generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB-derived MSCs is more or less similar to that of bone marrow-derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs.
authors.affiliation: Research Institute of Biotechnology, Histostem Co. 518-4 Taijul Bldg., Doonchun-dong, Kangdong-gu, Seoul 134-060, Korea.
authors.forename: Ju Ah
authors.initials: JA
authors.lastname: Jeong
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Hong
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Gang
authors.forename: Chiyoung
authors.initials: C
authors.lastname: Ahn
authors.forename: Soo Han
authors.initials: SH
authors.lastname: Hwang
authors.forename: Il Ho
authors.initials: IH
authors.lastname: Yang
authors.forename: Hoon
authors.initials: H
authors.lastname: Han
authors.forename: Hoeon
authors.initials: H
authors.lastname: Kim
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction