Effects of leptin on expression of acyl-coenzymea: cholesterol acyltransferases-1 in cultured human monocyte-macrophages.
To investigate the effects of leptin on expression of acyl-coenzymeA: cholesterol acyl-transferases-1 (ACAT-1) in monocyte-macrophage differentiation, human monocytic cells (THP-1) were cultured in RPMI 1640 and made to differentiate into macrophages under the incubation with phorbol myristate acetate (PMA) for 48 h. The cells were divided into 4 groups according to different intervention factors as follows: MCs cultured in RPM11640 medium with 10% FBS for 48 h served as MC group (control group), MCs cultured in medium with serum-free RPM11640 containing 5% BSA, 100 nmol/L PMA for 48 h as MP group, MCs cultured in RPMI1640 medium with 10% FBS, 10 micromol/ml leptin for 48 h as leptin-MC group, and MCs cultured in medium with serum-free RPMI1640 containing 5% BSA. 100 nmol/L PMA, and 10 micromol/ml leptin for 48 h as leptin-MP group. Immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and Western blot were performed, respectively, to observe the effects of leptin on expression of ACAT-1 in the monocyte-macrophage differentiation. Our results showed that expression of ACAT-1 protein and mRNA in MP-group is two times that in MC-group (P<0.05), and the expression of ACAT-1 protein and mRNA increased by up to 4 folds in leptin-MP group-as compared with that of MC group (P<0.01). Thus, our results support the idea that expression of ACAT-1 increases more in cultured human macrophages than in monocytes, and leptin can significantly promote ACAT-1 expression. It was concluded that high expression of ACAT-1 may accelerate the development of human atherogenesis, and leptin might participate in atherogenesis by increasing expression of ACAT-1.
Bai
Zhifeng
Z
Department of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China.
Cheng
Bei
B
Yu
Qizhen
Q
Li
Changyun
C
He
Ping
P
Mao
Xiaobo
X
D050197:Atherosclerosis / Q000201:enzymology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000201:enzymology*; D009000:Monocytes / Q000166:cytology / Q000201:enzymology*; D002785:Sterol O-Acyltransferase / Q000096:biosynthesis* / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate
D020738:Leptin; D002785:Sterol O-Acyltransferase; D013755:Tetradecanoylphorbol Acetate