[Production of recombinant fragment of VacA protein and development of non-invasive method for the diagnosis of Helicobacter pylori infection].
Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in the diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum. The thus designed coagulating test-system was successfully tested under the modeling conditions with clinical samples. Therefore, the designed express method can be used for the invasion-free determination of VacA in patients with gastric and duodenal pathologies.
Belyĭ
Iu F
IuF
Sheklakova
L A
LA
Vakhrameeva
M S
MS
Zhukhovitskiĭ
V G
VG
Petrukhin
V G
VG
Belaia
Iu A
IuA
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis / Q000097:blood / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; C074689:VacA protein, Helicobacter pylori