Noise propagation in gene networks.
Accurately predicting noise propagation in gene networks is crucial for understanding signal fidelity in natural networks and designing noise-tolerant gene circuits. To quantify how noise propagates through gene networks, we measured expression correlations between genes in single cells. We found that noise in a gene was determined by its intrinsic fluctuations, transmitted noise from upstream genes, and global noise affecting all genes. A model was developed that explains the complex behavior exhibited by the correlations and reveals the dominant noise sources. The model successfully predicts the correlations as the network is systematically perturbed. This approach provides a step toward understanding and manipulating noise propagation in more complex gene networks.
Pedraza
Juan M
JM
Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
van Oudenaarden
Alexander
A
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D056968:Lac Repressors; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008433:Mathematics; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013269:Stochastic Processes; D013754:Tetracyclines / Q000494:pharmacology; D014158:Transcription, Genetic; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D000097573:Red Fluorescent Protein
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins; D007544:Isopropyl Thiogalactoside; D056968:Lac Repressors; D008164:Luminescent Proteins; D012097:Repressor Proteins; D013754:Tetracyclines; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D000097573:Red Fluorescent Protein; C485182:Cyan Fluorescent Protein; C539064:LacI protein, E coli; C016649:phage repressor proteins; C034811:tetracycline resistance-encoding transposon repressor protein; C054562:yellow fluorescent protein, Bacteria; C016229:4-epianhydrotetracycline