title: Structured model for denitrifier diauxic growth.
abstract: We present a model for diauxic growth of denitrifying bacteria in which nitrate reductase synthesis kinetics dominate the overall growth kinetics. The model is based on the assumption of the existence of a nitrate respiration operon, thereby linking the rate of nitrate uptake to the activity of nitrate reductase. We show that this approach can model diauxic growth of Pseudomonas denitrificans by conducting experiments in which nitrate reductase activity was measured during both lag and ensuing exponential growth phases. We consistently observed the pattern of low nitrate reductase enzyme activity during the lag phase, increasing before the onset of growth. By fitting model parameters we were able to successfully match experimental data for growth, nitrate uptake, and enzyme activity level.
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Hamilton
authors.forename: Anna
authors.initials: A
authors.lastname: Casasús
authors.forename: Madeline
authors.initials: M
authors.lastname: Rasche
authors.forename: Atul
authors.initials: A
authors.lastname: Narang
authors.forename: Spyros A
authors.initials: SA
authors.lastname: Svoronos
authors.forename: Ben
authors.initials: B
authors.lastname: Koopman
mesh_terms: D008954:Models, Biological; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D009566:Nitrates; D009573:Nitrites; D009565:Nitrate Reductases; D050901:Nitrate Reductase