[Substrate properties of dinucleoside 5',5"-oligophosphates in the reactions catalyzed by HIV reverse transcriptase, E. coli DNA polymerase I, and E. coli RNA polymerase].
Dinucleoside 5',5"-oligophosphates dNp4dN', dTp3rA, dTp4rA, and dTp5rA were synthesized and evaluated as substrates for HIV reverse transcriptase, the Klenow fragment of E. coli DNA polymerase I, and E. coli RNA polymerase. A model of the HIV reverse transcriptase interaction with the dinucleoside oligophosphates was proposed and experimentally confirmed. The kinetic constants of some of the synthesized compounds for the HIV reverse transcriptase were determined.
D004256:DNA Polymerase I / Q000737:chemistry*; D015224:Dideoxynucleosides / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D006678:HIV / Q000201:enzymology*; D054303:HIV Reverse Transcriptase / Q000737:chemistry*; D008956:Models, Chemical; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D013379:Substrate Specificity
D015224:Dideoxynucleosides; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I