title: The first two-dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins.
abstract: Cytosolic proteins of Schizosaccharomyces pombe were separated by two-dimensional (2-D) gel electrophoresis, to construct the first 2-D reference map. In the pI range 4-7, more than 500 spots were detected by silver staining, and 70 different proteins corresponding to 111 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and tandem mass spectrometry, where necessary. In the pI range 6-9, approximately 330 spots were detected, and 31 proteins corresponding to 38 spots were identified by mass spectrometry. More than 50% of the identified proteins were involved in amino acid, carbohydrate or nucleotide metabolism, and energy production. A second large group of identified proteins comprises heat shock and other stress related proteins and chaperones.
authors.affiliation: Laboratory of Protein Chemistry, Chungnam National University, Daejeon, South Korea.
authors.forename: Namkyu
authors.initials: N
authors.lastname: Sun
authors.forename: Jaewon
authors.initials: J
authors.lastname: Jang
authors.forename: Seunghee
authors.initials: S
authors.lastname: Lee
authors.forename: Sunkyung
authors.initials: S
authors.lastname: Kim
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Kwang-Lae
authors.initials: KL
authors.lastname: Hoe
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Chung
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Hyang-Sook
authors.initials: HS
authors.lastname: Yoo
authors.forename: Misun
authors.initials: M
authors.lastname: Won
authors.forename: Kyung Bin
authors.initials: KB
authors.lastname: Song
mesh_terms: D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D029702:Schizosaccharomyces pombe Proteins