title: Mycoplasma pneumoniae infection increases airway collagen deposition in a murine model of allergic airway inflammation.
abstract: Mycoplasma pneumoniae (Mp) has been linked to chronic asthma. Airway remodeling (e.g., airway collagen deposition or fibrosis) is one of the pathological features of chronic asthma. However, the effects of respiratory Mp infection on airway fibrosis in asthma remain unclear. In the present study, we hypothesized that respiratory Mp infection may increase the airway collagen deposition in a murine model of allergic airway inflammation in part through upregulation of transforming growth factor (TGF)-beta1. Double (2 wk apart) inoculations of Mp or saline (control) were given to mice with or without previous allergen (ovalbumin) challenges. On days 14 and 42 after the last Mp or saline, lung tissue and bronchoalveolar lavage (BAL) fluid were collected for analyses of collagen and TGF-beta1 at protein and mRNA levels. In allergen-naïve mice, Mp did not alter airway wall collagen. In allergen-challenged mice, Mp infections did not change airway wall collagen deposition on day 14 but increased the airway collagen on day 42; this increase was accompanied by increased TGF-beta1 protein in the airway wall and reduced TGF-beta1 protein release from the lung tissue into BAL fluid. Our results suggest that Mp infections could modulate airway collagen deposition in a murine model of allergic airway inflammation with TGF-beta1 involved in the collagen deposition process.
authors.affiliation: Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA. chuhw@njc.org
authors.forename: Hong Wei
authors.initials: HW
authors.lastname: Chu
authors.forename: John G
authors.initials: JG
authors.lastname: Rino
authors.forename: Rachel B
authors.initials: RB
authors.lastname: Wexler
authors.forename: Krista
authors.initials: K
authors.lastname: Campbell
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Harbeck
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Martin
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003094:Collagen / Q000378:metabolism*; D004195:Disease Models, Animal; D005355:Fibrosis; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009177:Mycoplasma pneumoniae; D011019:Pneumonia, Mycoplasma / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D012137:Respiratory System; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1
chemical_list: C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen