Application of non-aqueous micellar electrokinetic chromatography to the analysis of active components in radix Salviae miltiorrhizae and its medicinal preparations.
A simple, economical and effective non-aqueous micellar electrokinetic chromatography (NAMEKC) method was developed for simultaneous assay of three bioactive components (cryptotanshinone, tanshinone IIA and tanshinone I) in radix Salviae miltiorrhizae and its medicinal preparations for the first time. After optimization of separation conditions, a buffer of 140 mmol l(-1) sodium cholate (SC) in methanol was selected for the separation of the three tanshinones, but baseline separation of tanshinone I and tanshinone IIA in practical samples was not achieved. Therefore, second-order derivative electropherograms were applied for resolving overlapping peaks. Regression equations revealed good linear relationships (correlation coefficients 0.995-0.999) between peak heights in second-order derivative electropherograms and concentrations of the three analytes. The recoveries of three constituents ranged from 91.3 to 105.7%. The results indicated that baseline separation of the analytes was hard to be achieved in practical samples sometimes and second-order derivative electropherograms was applicable for the resolving and analysis of overlapping peaks.
Chen
Anjia
A
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China. chenanjia888@163.com
Li
Cunhong
C
Gao
Wenhua
W
Hu
Zhide
Z
Chen
Xingguo
X
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D020374:Chromatography, Micellar Electrokinetic Capillary; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008823:Micelles; D012015:Reference Standards; D015203:Reproducibility of Results; D027544:Salvia / Q000737:chemistry*; D012997:Solvents; D013607:Tablets
D004365:Drugs, Chinese Herbal; D008823:Micelles; D012997:Solvents; D013607:Tablets