Genotype analysis of Campylobacter jejuni isolated from broilers in Poland.
Twenty-six Campylobacter jejuni strains isolated from poultry were analyzed by genotypic typing including ITS-profiling, REP- and ERIC-PCR. ITS-profiling revealed the presence of 8 different genotypes. Amplification of REP sequences by PCR gave similar results with 10 different genotypes. ERIC-PCR was found to be the most discriminatory for typing C. jejuni. As many as 13 different DNA patterns were obtained with this technique. Based on data obtained it was found that C. jejuni isolates recovered from broilers at the slaughterhouses in southwest Poland are characterized by a high degree of genetic heterogeneity.
Wojciech
L
L
Department of Biochemistry, Faculty of Veterinary Medicine Wrocław Agriculture University, Norwida 31, 50-375 Wrocław, Poland.
Bednarski
M
M
Wieliczko
A
A
Ugorski
M
M
D000818:Animals; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology*; D016172:DNA Fingerprinting; D021901:DNA, Intergenic / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D020869:Gene Expression Profiling; D005838:Genotype; D008099:Liver / Q000382:microbiology; D010802:Phylogeny; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D021901:DNA, Intergenic