title: Antimicrobial potential of medicaments used in healing process in dogs' teeth with apical periodontitis.
abstract: The objective of this study was to evaluate the influence of irrigants on the antimicrobial potential of calcium hydroxide paste in dogs' teeth with apical periodontitis. Forty-eight premolar teeth of adult mongrel dogs had their root canals opened to the oral environment for 6 months. The root canals were prepared and treated with different irrigating solutions and intracanal medicaments, according to the following groups: 1) 2.5% sodium hypochlorite (NaOCl) + calcium hydroxide paste (CHP); 2) 2% chlorhexidine (CHX) + CHP; 3) vinegar + CHP; 4) vinegar + vinegar. In group 4, both the irrigating solution and intracanal medicament were vinegar, which was renewed every 7 days. Each sample was collected by using two paper points maintained in position for 1 min, and individually transported and immersed in 7 mL of Letheen broth (LB), followed by incubation at 37 degrees C for 48 h. Microbial growth was analyzed by two methods: turbidity of the culture medium and subculture on a specific nutrient broth (brain heart infusion). After 21 days, all experimental groups had microbial growth, however, in different percentages: group 1 - 30%; group 2 - 30%; group 3 - 40%; group 4 - 60%. All materials tested had antimicrobial potential; however, the influence of calcium hydroxide paste on the control of microorganisms must be remembered.
authors.affiliation: Faculty of Dentistry, Federal University of Goiás, Goiânia, GO, Brazil. estrela3@terra.com.br
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
authors.forename: Roberto
authors.initials: R
authors.lastname: Holland
authors.forename: Pedro Felício Estrada
authors.initials: PF
authors.lastname: Bernabé
authors.forename: Valdir
authors.initials: V
authors.lastname: de Souza
authors.forename: Cyntia R A
authors.initials: CR
authors.lastname: Estrela
mesh_terms: D019342:Acetic Acid / Q000627:therapeutic use; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology; D004285:Dogs; D010485:Periapical Periodontitis / Q000382:microbiology / Q000628:therapy*; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide; D019342:Acetic Acid; D002710:Chlorhexidine