title: DNA array analysis of Candida albicans gene expression in response to adherence to polystyrene.
abstract: Candidiasis is often initiated by the colonization of inert surfaces. In order to elucidate the mechanisms involved in this adherence process, DNA macroarrays were used to analyze the transcriptome of Candida albicans, the main causative agent of this mycoses, in a simple adherence model using germ tubes produced in polystyrene Petri dishes. Non-adherent germ tubes produced on glass surface were used as a control. Analysis of gene expression displayed 77 genes identified as statistically overexpressed in adherent germ tubes. Among these genes, some encoded enzymes participating in metabolism of lipids (such as LIP6), of proteins (such as SAP1) or of carbohydrates (like PGI1, PMI40 and PSA1. Some of these genes have already been reported as playing a role in pathogenesis of C. albicans. However, functions were unknown for a large part (45.5%) of the overexpressed genes which will be analyzed further in order to define their relationship with adherence.
authors.affiliation: Groupe d'Etude des Interactions Hôte-Parasite, UPRES EA 3142, UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 16 Bd Daviers, 49045 Angers Cedex, France. veronique.marchais@univ-angers.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Marchais
authors.forename: Marie
authors.initials: M
authors.lastname: Kempf
authors.forename: Patricia
authors.initials: P
authors.lastname: Licznar
authors.forename: Corinne
authors.initials: C
authors.lastname: Lefrançois
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Bouchara
authors.forename: Raymond
authors.initials: R
authors.lastname: Robert
authors.forename: Jane
authors.initials: J
authors.lastname: Cottin
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005898:Glass; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011137:Polystyrenes; D020543:Proteome; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D011137:Polystyrenes; D020543:Proteome