New antibacterial peptide derived from bovine hemoglobin.
Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis after separation by reversed-phase HPLC. From this fraction a pure peptide was isolated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). This peptide correspond to the 107-136 fragment of the alpha chain of bovine hemoglobin. The minimum inhibitory concentrations (MIC) towards the four strains and its hemolytic activity towards bovine erythrocytes were determined. A MIC of 38 microM was reported against L. innocua and 76 microM for other various bacterial species. This peptide had no hemolytic activity up to 380 microM concentration.
Daoud
Rachid
R
Laboratoire de Technologie des Substances Naturelles, IUT A Lille I, BP 179, 59653 Villeneuve d'Ascq Cedex, France.
Dubois
Veronique
V
Bors-Dodita
Loredana
L
Nedjar-Arroume
Naima
N
Krier
Francois
F
Chihib
Nour-Eddine
NE
Mary
Patrice
P
Kouach
Mostafa
M
Briand
Gilbert
G
Guillochon
Didier
D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D004926:Escherichia coli / Q000187:drug effects; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006461:Hemolysis; D006868:Hydrolysis; D008087:Listeria / Q000187:drug effects; D016982:Micrococcus luteus / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides; C503955:hemoglobin (107-136), bovine