title: Reestablishment of a transgenic rabbit line by artificial insemination using cryopreserved semen.
abstract: The production of recombinant proteins in the milk of transgenic animals is an alternative to traditional cell culture methodology. Transgenic rabbits can serve in the small-scale production of recombinant proteins, underscoring the need to maintain valuable transgenic lines. In this study, the authors used cryopreserved transgenic rabbit semen to artificially inseminate does, demonstrating the utility of this method for the reestablishment of a transgenic rabbit herd.
authors.affiliation: GTC Biotherapeutics, Spencer, MA 01562, USA. Stephen.Blash@gtc-bio.com
authors.forename: Stephen
authors.initials: S
authors.lastname: Blash
authors.forename: Lihow
authors.initials: L
authors.lastname: Chen
authors.forename: Merry
authors.initials: M
authors.lastname: Harvey
authors.forename: William G
authors.initials: WG
authors.lastname: Gavin
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001947:Breeding / Q000379:methods; D015925:Cryopreservation; D005260:Female; D007315:Insemination, Artificial / Q000379:methods*; D008098:Litter Size; D008297:Male; D011817:Rabbits / Q000235:genetics; D012661:Semen / Q000166:cytology / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D014730:Veterinary Medicine / Q000379:methods