Chemokines differentially induce matrix metalloproteinase-3 and prostaglandin E2 in human articular chondrocytes.
OBJECTIVE
To explore prostaglandin (PG) E2 production by human articular chondrocytes induced by different chemokines.


METHODS
Human chondrocytes were enzymatically isolated from the articular cartilage of patients with rheumatoid arthritis (RA), osteoarthritis (OA) or traumatic fracture (N) who underwent total joint replacement. They were cultured in vitro as monolayers and then exposed to MCP-1, RANTES or SDF-1 for 24 h. Levels of PGE2 and MMP-3 in the culture supernatant were then immunoassayed.


RESULTS
PGE2 production was enhanced up to 2.7-fold in a subset of samples. Responses to different chemokines were heterogeneous even within the same disease groups. As previously reported, chemokines induced MMP-3 secretion by chondrocytes, but there was no significant correlation between levels of PGE2 and MMP-3.


CONCLUSIONS
We here document the presence of "responders" among OA, RA and normal chondrocytes that produce enhanced levels of PGE2 upon stimulation by chemokines. The relationship between chemokines and prostaglandins could differentially influence the pathogenic network responsible for cartilage degradation in arthropathy.
Masuko-Hongo
K
K
Department of Bioregulation, Institute of Medical Science, St. Marianna University School of Medicine, Kanagawa, Japan. khongo@marianna-u.ac.jp
Sato
T
T
Nishioka
K
K
D000368:Aged; D000369:Aged, 80 and over; D001168:Arthritis / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D019643:Arthroplasty, Replacement; D002358:Cartilage, Articular / Q000276:immunology; D018932:Chemokine CCL2 / Q000276:immunology; D018946:Chemokine CCL5 / Q000276:immunology; D054377:Chemokine CXCL12; D018925:Chemokines / Q000276:immunology*; D019743:Chemokines, CXC / Q000276:immunology; D019902:Chondrocytes / Q000276:immunology*; D015232:Dinoprostone / Q000276:immunology*; D005260:Female; D050723:Fractures, Bone / Q000276:immunology; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000276:immunology*; D008875:Middle Aged; D010003:Osteoarthritis / Q000276:immunology
C515255:CXCL12 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054377:Chemokine CXCL12; D018925:Chemokines; D019743:Chemokines, CXC; D019278:Matrix Metalloproteinase 3; D015232:Dinoprostone