title: The effect of simulated microgravity on hybridoma cells.
abstract: The effect of clinostat-simulated microgravity on SP-2/0 and 1D6 hybridoma cells was studied. Clinorotation during 4-5 days at 1.5 rounds per minute decreased dramatically their proliferating capacity: the rotated cells divided less than once while control cells performed 4-5 divisions. They decreased the non-specific adhesion to tissue culture plastic, but increased the number of cell-to-cell contacts. Such phenomenological changes were accompanied with the alterations in pericellular glycosaminoglycans: decreased accumulation of hyaluronic acid and increased accumulation of chondroitin/dermatan-sulfate, as well as with the increase of cytoplasmic Ca2+ concentration. Clinorotation resulted in hybridoma nicotinic receptor desensitization but not down-regulation. In contrast, both the quantity and quality (molecular isoforms, affinity and specificity) of the antibody produced by 1D6 hybridoma cells were not altered by clinorotation. It is concluded that simulated microgravity affected the proliferating and adhesive, but not biosynthetic properties of hybridoma cells.
authors.affiliation: Palladin Institute of Biochemistry, Kyiv, Ukraine. skok@biochem.kiev.ua
authors.forename: Marina V
authors.initials: MV
authors.lastname: Skok
authors.forename: Ludmila M
authors.initials: LM
authors.lastname: Koval
authors.forename: Yulia I
authors.initials: YI
authors.lastname: Petrova
authors.forename: Olena Y
authors.initials: OY
authors.lastname: Lykhmus
authors.forename: Denis V
authors.initials: DV
authors.lastname: Kolibo
authors.forename: Svitlana I
authors.initials: SI
authors.lastname: Romanyuk
authors.forename: Nataliya Y
authors.initials: NY
authors.lastname: Yevdokimova
authors.forename: Sergiy V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002807:Chondroitin / Q000096:biosynthesis; D003593:Cytoplasm / Q000502:physiology; D003871:Dermatan Sulfate / Q000096:biosynthesis; D015536:Down-Regulation / Q000502:physiology; D006112:Gravitation; D006820:Hyaluronic Acid / Q000096:biosynthesis; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009538:Nicotine / Q000494:pharmacology; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects; D012399:Rotation; D018474:Weightlessness Simulation
chemical_list: D011978:Receptors, Nicotinic; D003871:Dermatan Sulfate; D009538:Nicotine; D006820:Hyaluronic Acid; D002807:Chondroitin; D002118:Calcium