Reestablishment of a transgenic rabbit line by artificial insemination using cryopreserved semen.
The production of recombinant proteins in the milk of transgenic animals is an alternative to traditional cell culture methodology. Transgenic rabbits can serve in the small-scale production of recombinant proteins, underscoring the need to maintain valuable transgenic lines. In this study, the authors used cryopreserved transgenic rabbit semen to artificially inseminate does, demonstrating the utility of this method for the reestablishment of a transgenic rabbit herd.
Blash
Stephen
S
GTC Biotherapeutics, Spencer, MA 01562, USA. Stephen.Blash@gtc-bio.com
Chen
Lihow
L
Harvey
Merry
M
Gavin
William G
WG
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001947:Breeding / Q000379:methods; D015925:Cryopreservation; D005260:Female; D007315:Insemination, Artificial / Q000379:methods*; D008098:Litter Size; D008297:Male; D011817:Rabbits / Q000235:genetics; D012661:Semen / Q000166:cytology / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D014730:Veterinary Medicine / Q000379:methods