[Determination of calcium, magnesium and potassium in nurtured cell by AAS with quick-pulsed nebulization technique and NaOH base digestion].
The sample is digested with 6% NaOH solution and an amount of 50 microl is used for protein content analysis by the method of Comassie Brilliant Blue G250, the residual is diluted with equal 0.4% Lathanurm-EDTA solution. Its Calcium magensium and potassium content are determined by AAS. With quick-pulsed nebulization technique. When a self-made micro-sampling device is used, 20microl of sample volume is needed and it is only the 1/10 approximately 1/20 of the sample volume required for conventional determination. Sensitivity, precision and rate of recovery agree well with those using regular wet ashing method.
Shi
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C
Department of Military Hygienice, the Fourth Military Medical University, 710032 Xi'an.
Gao
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S
Gun
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S
D000818:Animals; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000737:chemistry / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000032:analysis; D008670:Metals / Q000032:analysis*; D009330:Nebulizers and Vaporizers; D011188:Potassium / Q000032:analysis; D011817:Rabbits; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D008670:Metals; D012972:Sodium Hydroxide; D008274:Magnesium; D011188:Potassium; D002118:Calcium