Diacylglycerol pyrophosphate is a second messenger of abscisic acid signaling in Arabidopsis thaliana suspension cells.
In plants, the importance of phospholipid signaling in responses to environmental stresses is becoming well documented. The involvement of phospholipids in abscisic acid (ABA) responses is also established. In a previous study, we demonstrated that the stimulation of phospholipase D (PLD) activity and plasma membrane anion currents by ABA were both required for RAB18 expression in Arabidopsis thaliana suspension cells. In this study, we show that the total lipids extracted from ABA-treated cells mimic ABA in activating plasmalemma anion currents and induction of RAB18 expression. Moreover, ABA evokes within 5 min a transient 1.7-fold increase in phosphatidic acid (PA) followed by a sevenfold increase in diacylglycerol pyrophosphate (DGPP) at 20 min. PA activated plasmalemma anion currents but was incapable of triggering RAB18 expression. By contrast, DGPP mimicked ABA on anion currents and was also able to stimulate RAB18 expression. Here we show the role of DGPP as phospholipid second messenger in ABA signaling.
Zalejski
Christine
C
Physiologie Cellulaire et Moléculaire des Plantes, Unité Mixte de Recherche 7632-Centre National de la Recherche Scientifique, case 156, Université Pierre et Marie Curie, Le Raphaël, 3 rue Galilée 94200 Ivry sur Seine, France.
Zhang
Zongshen
Z
Quettier
Anne-Laure
AL
Maldiney
Régis
R
Bonnet
Magda
M
Brault
Mathias
M
Demandre
Chantal
C
Miginiac
Emile
E
Rona
Jean-Pierre
JP
Sotta
Bruno
B
Jeannette
Emmanuelle
E
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002478:Cells, Cultured; D011756:Diphosphates / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005990:Glycerol / Q000031:analogs & derivatives* / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D015290:Second Messenger Systems; D020691:rab GTP-Binding Proteins / Q000378:metabolism
D029681:Arabidopsis Proteins; D011756:Diphosphates; D010712:Phosphatidic Acids; C408642:diacylglycerol pyrophosphate; C078703:rab18 protein, Arabidopsis; D000040:Abscisic Acid; D020691:rab GTP-Binding Proteins; D005990:Glycerol