Role of gap junctional intercellular communication (GJIC) through p38 and ERK1/2 pathway in the differentiation of rat neuronal stem cells.
Gap junctional intercellular communications (GJIC) contributes to neural function in development and differentiation of CNS. In this study, we have investigated the expression of GJIC during the differentiation of neuronal stem cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced neuronal stem cell-derived cells from rat brain. During neuronal stem cell differentiation, expressions of Cx43 and 32 were increased for the duration of 72 hr, however the effect were decreased on the 7d. In the neuronal stem cell-derived cells, pretreatments with p38 MAP kinase inhibitor, SB203580, and MEK inhibitor, PD98059, could protect GJIC against TPA-induced inhibition of GJIC. Our data suggest that GJIC plays an important role during neuronal stem cell differentiation, and ERK1/2 and p38 MAP kinase signaling pathway may be closely related functionally to regulate gap junction in rat neuronal stem cell-derived cells.
Yang
Se-Ran
SR
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea.
Cho
Sung-Dae
SD
Ahn
Nam-Shik
NS
Jung
Ji-Won
JW
Park
Joon-Suk
JS
Jo
Eun-Hye
EH
Hwang
Jae-Woong
JW
Jung
Ji-Youn
JY
Kim
Tae-Yung
TY
Yoon
Byoung-Su
BS
Lee
Bong-Hee
BH
Kang
Kyung-Sun
KS
Lee
Yong-Soon
YS
D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D018031:Connexin 43 / Q000378:metabolism; D017630:Connexins / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D017629:Gap Junctions / Q000502:physiology*; D007093:Imidazoles / Q000494:pharmacology; D009474:Neurons / Q000166:cytology*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats / Q000196:embryology*; D013234:Stem Cells / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate; D000097002:Gap Junction beta-1 Protein
D018031:Connexin 43; D017630:Connexins; D005419:Flavonoids; D007093:Imidazoles; D011725:Pyridines; D013755:Tetradecanoylphorbol Acetate; D000097002:Gap Junction beta-1 Protein; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one