title: The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1.
abstract: Cohesion established between sister chromatids during pre-meiotic DNA replication mediates two rounds of chromosome segregation. The first division is preceded by an extended prophase wherein homologous chromosomes undergo recombination. The persistence of cohesion during prophase is essential for recombination and both meiotic divisions. Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis. During S- and prophase, Mnd2 prevents activation of the APC/C by a meiosis-specific activator called Ama1. In cells lacking Mnd2 the APC/C-Ama1 enzyme triggers degradation of Pds1, which causes premature sister chromatid separation due to unrestrained separase activity. In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Oelschlaegel
authors.forename: Martin
authors.initials: M
authors.lastname: Schwickart
authors.forename: Joao
authors.initials: J
authors.lastname: Matos
authors.forename: Aliona
authors.initials: A
authors.lastname: Bogdanova
authors.forename: Alain
authors.initials: A
authors.lastname: Camasses
authors.forename: Jan
authors.initials: J
authors.lastname: Havlis
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zachariae
mesh_terms: D000705:Anaphase / Q000235:genetics / Q000502:physiology; D064173:Anaphase-Promoting Complex-Cyclosome; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D017489:CDC28 Protein Kinase, S cerevisiae / Q000235:genetics / Q000378:metabolism; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002842:Chromatids / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics / Q000502:physiology*; D010450:Endopeptidases / Q000378:metabolism; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011489:Protein Denaturation / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D064249:Securin; D064247:Separase; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism*
chemical_list: C577515:Ama1 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; C577468:MND2 protein, S cerevisiae; D009687:Nuclear Proteins; C578004:PDS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D016203:CDC2 Protein Kinase; D017489:CDC28 Protein Kinase, S cerevisiae; D010450:Endopeptidases; C577986:ESP1 protein, S cerevisiae; D064247:Separase