title: The invertor knock-in conditional chromosomal translocation mimic.
abstract: Knock-in models of tumor-specific chromosomal translocations can generate lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Forster
authors.forename: Richard
authors.initials: R
authors.lastname: Pannell
authors.forename: Lesley F
authors.initials: LF
authors.lastname: Drynan
authors.forename: Rosalind
authors.initials: R
authors.lastname: Codrington
authors.forename: Angelika
authors.initials: A
authors.lastname: Daser
authors.forename: Markus
authors.initials: M
authors.lastname: Metzler
authors.forename: M Natividad
authors.initials: MN
authors.lastname: Lobato
authors.forename: Terence H
authors.initials: TH
authors.lastname: Rabbitts
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002469:Cell Separation; D002875:Chromosomes / Q000648:ultrastructure*; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D005821:Genetic Techniques; D006801:Humans; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000378:metabolism; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014178:Translocation, Genetic
chemical_list: D018076:DNA, Complementary; D012313:RNA