title: Immunolocalization and expression of kinin B1R and B2R receptors in human inflammatory bowel disease.
abstract: Bradykinin is a mediator of inflammation, responsible for pain, vasodilation, and capillary permeability. Bradykinin receptor 1 (B(1)R) and bradykinin receptor 2 (B(2)R) are G protein-coupled receptors that mediate kinin effects. The latter is constitutive and rapidly desensitized; the former is induced by inflammatory cytokines and resistant to densensitization. The distribution of bradykinin receptors in human intestinal tissue was studied in patients with inflammatory bowel disease (IBD), namely ulcerative colitis (UC) and Crohn's disease (CD). Both B(2)R and B(1)R proteins are expressed in the epithelial cells of normal and IBD intestines. B(1)R protein is visualized in macrophages at the center of granulomas in CD. B(2)R protein is normally present in the apexes of enterocytes in the basal area and intracellularly in inflammatory tissue. In contrast, B(1)R protein is found in the basal area of enterocytes in normal intestine but in the apical portion of enterocytes in inflamed tissue. B(1)R protein is significantly increased in both active UC and CD intestines compared with controls. In patients with active UC, B(1)R mRNA is significantly higher than B(2)R mRNA. However, in inactive UC patients, the B(1)R and B(2)R mRNA did not differ significantly. Thus bradykinin receptors in IBD may reflect intestinal inflammation. Increased B(1)R gene and protein expression in active IBD provides a structural basis of the important role of bradykinin in chronic inflammation.
authors.affiliation: Department of Internal Medicine, Medical University of Silesia, Katowice, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Stadnicki
authors.forename: Ezbieta
authors.initials: E
authors.lastname: Pastucha
authors.forename: Grazyna
authors.initials: G
authors.lastname: Nowaczyk
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Danuta
authors.initials: D
authors.lastname: Plewka
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Machnik
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
authors.forename: Robert W
authors.initials: RW
authors.lastname: Colman
mesh_terms: D000328:Adult; D000906:Antibodies; D003093:Colitis, Ulcerative / Q000378:metabolism / Q000503:physiopathology*; D003424:Crohn Disease / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007422:Intestines / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D043783:Receptor, Bradykinin B1 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D043782:Receptor, Bradykinin B2 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2