Effects of N-acetylcysteine treatment on oxidative stress in acetic acid-induced experimental colitis in rats.
We assessed the possible protective effects of N-acetylcysteine (NAC) against toxic damage in the rat colon. Two doses of NAC (20 mg/kg and 100 mg/kg) given for 2 days and 7 days after acetic acid administration (to induce colitis) were tested. NAC was dissolved in saline and administered locally (intracolonic), systemically (intraperitoneal) or in a combination (intracolonic and intraperitoneal). Several parameters, including macroscopic and histopathological scores and myeloperoxidase, glutathione and nitric oxide concentrations were measured using standard assay procedures. Treatment with 100 mg/kg NAC for 7 days significantly decreased tissue myeloperoxidase, glutathione and nitric oxide concentrations. The 20 mg/kg dose had no protective effects. The data indicate that NAC substantially reduced the degree of colonic injury, probably by regulating free radical production and inhibiting inflammation. It may, therefore, have a role in the treatment of inflammatory bowel disease.
Akgun
E
E
Department of General Surgery, Ege University School of Medicine, Bornova, Izmir, Turkey. eakgun@med.ege.edu.tr
Caliskan
C
C
Celik
H A
HA
Ozutemiz
A O
AO
Tuncyurek
M
M
Aydin
H H
HH
D019342:Acetic Acid / Q000494:pharmacology*; D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D003092:Colitis / Q000139:chemically induced*; D003106:Colon / Q000201:enzymology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D007249:Inflammation; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D005609:Free Radicals; D009569:Nitric Oxide; D012965:Sodium Chloride; D009195:Peroxidase; D005978:Glutathione; D019342:Acetic Acid; D000111:Acetylcysteine