Production of 3-hydroxy-n-phenylalkanoic acids by a genetically engineered strain of Pseudomonas putida.
Overexpression of the gene encoding the poly-3-hydroxy-n-phenylalkanoate (PHPhA) depolymerase (phaZ) in Pseudomonas putida U avoids the accumulation of these polymers as storage granules. In this recombinant strain, the 3-OH-acyl-CoA derivatives released from the different aliphatic or aromatic poly-3-hydroxyalkanoates (PHAs) are catabolized through the beta-oxidation pathway and transformed into general metabolites (acetyl-CoA, succinyl-CoA, phenylacetyl-CoA) or into non-metabolizable end-products (cinnamoyl-CoA). Taking into account the biochemical, pharmaceutical and industrial interest of some PHA catabolites (i.e., 3-OH-PhAs), we designed a genetically engineered strain of P. putida U (P. putida U DeltafadBA-phaZ) that efficiently bioconverts (more than 80%) different n-phenylalkanoic acids into their 3-hydroxyderivatives and excretes these compounds into the culture broth.
Sandoval
Angel
A
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 Leon, Spain. dbbjlr@unileon.es
Arias-Barrau
Elsa
E
Bermejo
Francisco
F
Cañedo
Librada
L
Naharro
Germán
G
Olivera
Elías R
ER
Luengo
José M
JM
D000144:Acids, Acyclic / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D001704:Biopolymers / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D006880:Hydroxy Acids / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
D000144:Acids, Acyclic; D000473:Alkanes; D001704:Biopolymers; D002264:Carboxylic Acids; D006880:Hydroxy Acids; D002265:Carboxylic Ester Hydrolases; C077226:poly(3-hydroxyalkanoic acid) depolymerase