Spin label EPR-based characterization of biosystem complexity.
Following the widely spread EPR spin-label applications for biosystem characterization, a novel approach is proposed for EPR-based characterization of biosystem complexity. Hereto a computational method based on a hybrid evolutionary optimization (HEO) is introduced. The enormous volume of information obtained from multiple HEO runs is reduced with a novel so-called GHOST condensation method for automatic detection of the degree of system complexity through the construction of two-dimensional solution distributions. The GHOST method shows the ability of automatic quantitative characterization of groups of solutions, e.g. the determination of average spectral parameters and group contributions. The application of the GHOST condensation algorithm is demonstrated on four synthetic examples of different complexity and applied to two physiologically relevant examples--the determination of domains in biomembranes (lateral heterogeneity) and the study of the low-resolution structure of membrane proteins.
Strancar
Janez
J
Laboratory of Biophysics, JoZef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. janez.strancar@ijs.si
Koklic
Tilen
T
Arsov
Zoran
Z
Filipic
Bogdan
B
Stopar
David
D
Hemminga
Marcus A
MA
D000465:Algorithms; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D013113:Spin Labels
D008565:Membrane Proteins; D013113:Spin Labels