Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture.
Applying a newly developed user-interactive optical trapping system, we controllably surrounded individual cells of one yeast species, Hanseniaspora uvarum, with viable cells of another yeast species, Saccharomyces cerevisiae, thus creating a confinement of the former. Growth of surrounded and non-surrounded H. uvarum cells was followed under a microscope by determining their generation time. The average generation time of surrounded H. uvarum cells was 15% higher than that of non-surrounded cells, thereby showing that the confinement imposed by viable S. cerevisiae cells on H. uvarum inhibits growth of the latter. This study is the first to demonstrate that confinement is a determinant of growth in a microbial ecosystem.
Arneborg
Nils
N
Department of Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
Siegumfeldt
Henrik
H
Andersen
Grith H
GH
Nissen
Peter
P
Daria
Vincent R
VR
Rodrigo
Peter John
PJ
Glückstad
Jesper
J
D003470:Culture Media; D017753:Ecosystem; D018869:Heat-Shock Response; D007091:Image Processing, Computer-Assisted; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D004718:Saccharomycetales / Q000254:growth & development*
D003470:Culture Media