title: Analysis of aberrant somatic hypermutation (SHM) in non-Hodgkin's lymphomas of patients with chronic HCV infection.
abstract: Hepatitis C virus (HCV) and aberrant somatic hypermutation (SHM) have each been suggested to contribute to the development of B-cell non-Hodgkin's lymphoma (NHL). The incidence of PIM-1, PAX-5, RhoH/TTF, and c-MYC mutations in tumour biopsy specimens from 32 HCV-infected B-cell NHL patients was analysed to determine whether the extent of aberrant SHM among these patients differed from that previously reported for HCV-negative B-cell NHL patients. Mutation of PIM-1, PAX-5, RhoH/TTF, and c-MYC was detected in 4 (13%), 5 (16%), 4 (13%), and 4 (13%) of 32 samples, respectively. In HCV-positive B-cell NHL patients, the frequency of aberrant SHM was lower than that already found in HCV-negative B-cell NHL patients. This indicates that, unlike B-cell lymphomas from HCV-negative patients, aberrant SHM may not contribute significantly to malignant transformation in HCV-associated B-cell lymphomas.
authors.affiliation: Experimental Oncology 1, Centro di Riferimento Oncologico, Istituto Nazionale Tumori, Istituto di Ricovero e Cura a Carattere Scientifico, Aviano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Libra
authors.forename: Daniela
authors.initials: D
authors.lastname: Capello
authors.forename: Annunziata
authors.initials: A
authors.lastname: Gloghini
authors.forename: Pasqualucci
authors.initials: P
authors.lastname: Laura
authors.forename: Eva
authors.initials: E
authors.lastname: Berra
authors.forename: Michaela
authors.initials: M
authors.lastname: Cerri
authors.forename: Daniela
authors.initials: D
authors.lastname: Gasparotto
authors.forename: Stivala
authors.initials: S
authors.lastname: Franca
authors.forename: Valli
authors.initials: V
authors.lastname: De Re
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gaidano
authors.forename: Antonino
authors.initials: A
authors.lastname: Carbone
mesh_terms: D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D016259:Genes, myc; D016174:Hepacivirus; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000235:genetics* / Q000821:virology*; D051757:PAX5 Transcription Factor; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D051573:Proto-Oncogene Proteins c-pim-1; D027041:Somatic Hypermutation, Immunoglobulin; D014157:Transcription Factors / Q000235:genetics; D020741:rho GTP-Binding Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; C447841:RhoH protein, human; D014157:Transcription Factors; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1; D020741:rho GTP-Binding Proteins