title: Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
abstract: Abstract Heat shock proteins (Hsp) can deliver antigen into the major histocompatibility complex class I presentation pathway of antigen-presenting cells (APC), a process called cross priming, thus stimulating antigen-specific CD8+ T-cell reactions. Hsp were shown to elicit proinflammatory responses in APC. Both processes require interaction of Hsp with APC via specific receptors. This study describes the interaction of recombinant Hsp70 (rHsp70) of Mycobacterium avium subspecies paratuberculosis with bovine peripheral blood mononuclear cells that was restricted to CD14+ cells. Characterized monocyte-derived macrophages, monocyte-derived dendritic cells (DC) and BoMac, an immortalized bovine macrophage cell line, were used to investigate the interaction of rHsp70 with different bovine APC. Saturation of immature DC with high concentrations of rHsp70 is demonstrated, and it was found that interaction of rHsp70 with DC was related to the maturation stage of the DC. Involvement of CD91 as a cellular receptor for rHsp70 was demonstrated; however, competition studies with immature DC demonstrated that other receptors exist on bovine APC. These data suggest that rHsp70-based vaccines may be useful for the successful immunization of cattle.
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, Netherlands. m.f.m.langelaar@vet.uu.nl
authors.forename: M F M
authors.initials: MF
authors.lastname: Langelaar
authors.forename: J C
authors.initials: JC
authors.lastname: Hope
authors.forename: V P M G
authors.initials: VP
authors.lastname: Rutten
authors.forename: J P T M
authors.initials: JP
authors.lastname: Noordhuizen
authors.forename: W
authors.initials: W
authors.lastname: van Eden
authors.forename: A P
authors.initials: AP
authors.lastname: Koets
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000494:pharmacology; D001667:Binding, Competitive; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D002454:Cell Differentiation; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000276:immunology / Q000517:prevention & control; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018840:HSP70 Heat-Shock Proteins; D018950:Lipopolysaccharide Receptors; D000511:alpha-Macroglobulins