Adiponectin replacement therapy attenuates myocardial damage in leptin-deficient mice with viral myocarditis.
The effects of adiponectin replacement therapy on myocardial damage were studied in leptin-deficient (OB) mice with acute viral myocarditis. Encephalomyocarditis virus was injected intraperitoneally into OB and wild-type (WT) mice. One subgroup of OB mice received no intervention and another subgroup received daily adiponectin replacement, simultaneously with viral inoculation. Differences in heart weight, cardiac histological score, numbers of infiltrating or apoptotic cells in the myocardium and the immunoreactivity of adiponectin receptors in myocytes were determined. The reactivity of adiponectin receptor 1 in myocytes from OB mice on day 4 and day 8 after viral inoculation was significantly decreased compared with that in myocytes from WT mice; the OB mice also had elevated cardiac weights and severe inflammatory myocardial damage. Adiponectin replacement in OB mice inhibited the development of severe myocarditis by augmenting myocyte adiponectin receptor 1 reactivity. Exogenously administered adiponectin may inhibit the progression of viral myocarditis through binding to the adiponectin receptor 1 in leptin-deficient conditions.
Takahashi
T
T
Department of General Medicine, Kanazawa Medical University, Kahoku-gun, Ishikawa, Japan.
Saegusa
S
S
Sumino
H
H
Nakahashi
T
T
Iwai
K
K
Morimoto
S
S
Kanda
T
T
D052242:Adiponectin; D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D018450:Disease Progression; D004686:Encephalomyocarditis virus / Q000378:metabolism*; D015316:Genetic Therapy; D006321:Heart / Q000502:physiology / Q000821:virology; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000378:metabolism; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D032342:Muscle Cells / Q000378:metabolism; D009205:Myocarditis / Q000628:therapy* / Q000821:virology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size; D011485:Protein Binding; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000378:metabolism; D054411:Receptors, Leptin; D013997:Time Factors
D052242:Adiponectin; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C475419:adiponectin receptor 1, mouse; C515447:leptin receptor, mouse