The inhibition of diacylglycerol acyltransferase by terpenoids from Youngia koidzumiana.
The EtOAc extract of Youngia koidzumiana significantly inhibited the diacylglycerol acyltransferase (DGAT) from rat liver microsomes. Bioactivity-guided fractionation led to the isolation of nine compounds, the structures of which were established using physicochemical and spectral data. Of the isolated compounds, oleanolic acid (2), methyl ursolate (7) and corosolic aicd (8) inhibited DGAT, with IC50 values of 31.7, 26.4, and 44.3 microM, respectively. However, sesquiterpenoids showed only weak inhibitory effects toward DGAT.
Dat
Nguyen Tien
NT
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Cai
Xing Fu
XF
Rho
Mun-Chual
MC
Lee
Hyun Sun
HS
Bae
KiHwan
K
Kim
Young Ho
YH
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D051048:Diacylglycerol O-Acyltransferase; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013729:Terpenes / Q000302:isolation & purification / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013729:Terpenes; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase