The hyperresponsiveness of W/W(v) mice to oral sensitization is associated with a decrease in TCRgammadelta-T cells.
We have already reported that WBB6F1-W/W(v) (W/W(v)) mice, which have mutations in the c-kit gene, are highly susceptible to oral sensitization, and that the proportion of TCRgammadelta-T cells among the intraepithelial lymphocytes (IELs) (gammadelta-IELs) of W/W(v) is much lower than in congenic wild-type (+/+) mice. In this study we examined an inhibitory role of gammadelta-IELs in oral sensitization using two different methods. First, wild-type (+/+) mice were sensitized by oral administration of 1.0 mg ovalbumin (OVA) by gavage every day for 9 weeks after anti-TCRgammadelta antibody treatment 4 times. The treatment resulted in an enhanced OVA-specific IgG1 antibody production, active systemic anaphylaxis (ASA), and Th2-dominant cytokine production. Next, W/W(v) mice whose bone marrow cells were reconstituted from C57BL/6J mice for 5 months were sensitized by oral administration of OVA. The OVA-specific IgG1 antibody titer in the bone marrow-reconstituted W/W(v) mice was neither significantly enhanced, nor ASA was induced. The proportion of gammadelta-IELs in the reconstituted mice was much higher than that in the untreated W/W(v) mice. The above findings suggest that the decrease or increase in number of gammadelta-IELs enhances or decreases oral sensitization respectively. These results show that gammadelta-IELs have an important role in the oral tolerance to food antigens.
Okunuki
Haruyo
H
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Japan.
Teshima
Reiko
R
Sato
Yuji
Y
Nakamura
Ryosuke
R
Akiyama
Hiroshi
H
Maitani
Tamio
T
Sawada
Jun-Ichi
J
D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000941:Antigens / Q000008:administration & dosage; D001831:Body Temperature; D001854:Bone Marrow Cells / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000235:genetics; D018928:Immunity, Mucosal; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D010047:Ovalbumin / Q000276:immunology; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D000941:Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta; D010047:Ovalbumin; D019009:Proto-Oncogene Proteins c-kit