Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse.
To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders.
Schuler
Michael
M
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur, Collège de France, Illkirch-Cedex, France.
Ali
Faisal
F
Metzger
Elisabeth
E
Chambon
Pierre
P
Metzger
Daniel
D
D000199:Actins / Q000235:genetics*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D051927:CD24 Antigen; D002460:Cell Line; D022201:Chromosomes, Artificial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000196:embryology* / Q000254:growth & development*; D016296:Mutagenesis; D011960:Receptors, Estrogen / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000199:Actins; D015703:Antigens, CD; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D008562:Membrane Glycoproteins; D011960:Receptors, Estrogen; C045073:Cre recombinase; D019426:Integrases