Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography.
Electron tomography (ET) is a new technique for high resolution, three-dimensional (3D) reconstruction of pleiomorphic macromolecular complexes, such as virus components. By employing this technique, we resolved the 3D structure of Ebola virus nucleocapsid-like (NC-like) structures in the cytoplasm of cells expressing NP, VP24, and VP35: the minimum components required to form these NC-like structures. Reconstruction of these tubular NC-like structures of Ebola virus showed them to be composed of left-handed helices spaced at short intervals, which is structurally consistent with other non-segmented negative-strand RNA viruses.
Noda
Takeshi
T
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Aoyama
Kazuhiro
K
Sagara
Hiroshi
H
Kida
Hiroshi
H
Kawaoka
Yoshihiro
Y
D002478:Cells, Cultured; D029043:Ebolavirus / Q000648:ultrastructure*; D006801:Humans; D019251:Nucleocapsid / Q000737:chemistry*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D014758:Viral Core Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; C446938:VP24 protein, Ebola virus; C080980:VP35 protein, filovirus; D014758:Viral Core Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins