title: [Determination of sennoside A in rhubarb extracts by HPLC].
abstract: OBJECTIVE
  To determine sennoside A in rhubarb extracts by solvent extraction and SFE-CO2.
  
  
  METHODS
  Sennoside A was extracted by ultrasonic vibration. The analytical column was Allitima RP C18 (250 x 4.6 mm, 5 microm). The mobile phase consisted of 0.1% TFA-HCN (44:56). The flow rate was 1.0 ml/min. The UV detector wavelength was 280 nm and the reference wavelength was 600 nm. The column temperature was 25 degrees C.
  
  
  RESULTS
  Considering the extracting rate of sennoside A, solvent extraction was better than SFE-CO2.
authors.affiliation: Guangdong Pharmaceutical Association, Guangzhou.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zheng
authors.forename: Chenchen
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000880:Anthraquinones / Q000032:analysis* / Q000302:isolation & purification; D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012676:Senna Extract; D000081226:Sennosides; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D000081226:Sennosides; D002245:Carbon Dioxide; D012676:Senna Extract