title: Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity.
abstract: Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.
authors.affiliation: Department of Chemistry, American University, Washington, DC 20016, USA. Donohue.maura@epa.gov
authors.forename: Maura J
authors.initials: MJ
authors.lastname: Donohue
authors.forename: Mary B
authors.initials: MB
authors.lastname: Satterfield
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Dalluge
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Welch
authors.forename: James E
authors.initials: JE
authors.lastname: Girard
authors.forename: David M
authors.initials: DM
authors.lastname: Bunk
mesh_terms: D002021:Buffers; D003959:Diamines; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D017430:Prostate-Specific Antigen / Q000235:genetics*; D020033:Protein Isoforms / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D002021:Buffers; D003959:Diamines; D020033:Protein Isoforms; C009475:trimethylenediamine; D017430:Prostate-Specific Antigen