The application of quantum dots as fluorescent label to glycoarray.
A sensitive, specific, and rapid method for the detection of carbohydrate-protein interactions was demonstrated using quantum dots (QDs) as a fluorescence label coupled with protein. 1,3-Dipolar cycloaddition between azide and alkyne was exploited to attach alpha-d-glucopyranoside to a C(14) hydrocarbon chain that noncovalently binds to the microtiter well surface, and the product formation was detected by both electrospray ionization-mass spectrometry (ESI-MS) and QD- (or fluorescein isothiocyanate (FITC))-conjugated lectin binding. It indicated that the peak intensity of the fluorescence emission was proportional to the initial concanavalin A (Con A) concentration in the range of 2 x 10(-3) micromol/L to 2 x 10(-2)mmol/L with a detection limit at least 100 times lower than that of the FITC-based method.
Huang
Gang-Liang
GL
Key Laboratory of Biomedical Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China. hgl226@126.com
Liu
Tian-Cai
TC
Liu
Man-Xi
MX
Mei
Xin-Ya
XY
D000327:Adsorption; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005453:Fluorescence; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D011485:Protein Binding; D045663:Quantum Dots; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling / Q000379:methods*
D002241:Carbohydrates; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate