Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle.
Hyaluronan binding protein 1 (HABP1) is a negatively charged multifunctional mammalian protein with a unique structural fold. Despite the fact that HABP1 possesses mitochondrial localization signal, it has also been localized to other cellular compartments. Using indirect immunofluorescence, we examined the sub-cellular localization of HABP1 and its dynamics during mitosis. We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell. Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis. This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan, suggesting that in concert with each other the two molecules play critical roles in this dynamic process.
Sengupta
Aniruddha
A
Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Banerjee
Bhaswati
B
Tyagi
Rakesh K
RK
Datta
Kasturi
K
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006056:Golgi Apparatus / Q000378:metabolism*; D018960:Hyaluronan Receptors / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008938:Mitosis
D018960:Hyaluronan Receptors; D024101:Mitochondrial Proteins