Protein synthesis-dependent LTP in isolated dendrites of CA1 pyramidal cells.
Local dendritic protein synthesis provides a mechanism by which the effects of gene expression can be spatially restricted within synaptodendritic compartments of neurons. In the present study, we show that long-term potentiation (LTP), induced by two strong tetanic trains, can be produced in isolated CA1 apical dendrites and that new protein synthesis is critical for this dendritic LTP. LTP in isolated dendrites is also blocked by rapamycin, an inhibitor of growth-related protein synthesis, similar to that observed for LTP in the intact hippocampal slice. These results show that increased dendritic translation of proteins is a critical molecular mechanism for hippocampal LTP.
Cracco
Joan B
JB
Department of Physiology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA.
Serrano
Peter
P
Moskowitz
Shaye I
SI
Bergold
Peter J
PJ
Sacktor
Todd Charlton
TC
D000818:Animals; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D015870:Gene Expression; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017966:Pyramidal Cells / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D020123:Sirolimus / Q000494:pharmacology
D011500:Protein Synthesis Inhibitors; D020123:Sirolimus