title: Application of ITS sequence analysis, RAPD and AFLP fingerprinting in characterising the yeast genus Fellomyces.
abstract: Three molecular techniques, ITS sequence analysis, random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to study phylogenetic and genotypic relationships among strains of the genus Fellomyces. In the analyses were included strains isolated predominantly from epiphytic lichens collected in Indonesia, China and Mexico. The polyphasic approach indicated that the Fellomyces isolates are genotypically heterogeneous and that lichens represent a specific environment for selection of large number of the sterigmatoconidia producing species. The phylogenetic and genotypic analysis confirmed the existence of 11 currently accepted Fellomyces species and indicated that several species may be the new representatives of the genus. The RAPD and AFLP analyses demonstrated a higher potential in distinguishing the Fellomyces strains than the ITS regions. Since the sequence analysis showed low or no divergence among several strains, both RAPD and AFLP fingerprinting indicated that the strains may be discriminated at the species level.
authors.affiliation: Austrian Centre of Biological Resources and Applied Mycology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Ksenija
authors.initials: K
authors.lastname: Lopandic
authors.forename: Orsolya
authors.initials: O
authors.lastname: Molnár
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Prillinger
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004251:DNA Transposable Elements