title: Biological properties of Roakin strain of NDV and TK900 strain of ADV after serial passages in CECC in the presence of methisoprinol and KLP-602.
abstract: Twenty serial passages of the TK900 strain of Aujeszky's disease virus (ADV) and the Roakin strain of Newcastle disease virus (NDV) were made in a chicken embryo cell culture (CECC), in the presence of two antiviral agents: Methisoprinol and KLP-602. The physicochemical properties of passaged viruses were determined. The results obtained suggest that Methisoprinol causes changes in the structure of viral proteins, whereas KLP-602 affects the envelope-dependent properties of the virus. It was also found that the alternations observed in passaged viruses were temporary phenotypic changes only, and not a consequence of permanent transformations of their genotypes.
authors.affiliation: Department of Infectious and Invasive Diseases, Chair of Clinical Microbiology and Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland. stenia@uwm.edu.pl
authors.forename: J
authors.initials: J
authors.lastname: Małaczewska
authors.forename: Z
authors.initials: Z
authors.lastname: Rotkiewicz
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004986:Ether; D005838:Genotype; D011558:Herpesvirus 1, Suid / Q000187:drug effects* / Q000502:physiology; D007542:Inosine Pranobex / Q000494:pharmacology*; D009113:Muramidase / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000187:drug effects* / Q000502:physiology; D010641:Phenotype; D010740:Phospholipases; D012692:Serial Passage; D013696:Temperature; D014508:Urea; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004986:Ether; D014508:Urea; D010740:Phospholipases; C488571:KLP602; D009113:Muramidase; D007542:Inosine Pranobex