Electrochemical studies of danthron and the DNA-danthron interaction.
Danthron is an important natural occurring component in laxative drugs. In this paper, electrochemical investigation of danthron and its interaction with DNA is reported. Via the electrochemical approach assisted by ultraviolet-visible (UV-Vis) spectroscopy, we have proved that danthron intercalates into DNA strands forming some nonelectroactive complexes, which results in the decrease of redox peak currents of danthron. In addition, the decrease of the peak currents is proportional to the concentration of DNA. The difference between the interaction of danthron with double-stranded DNA (dsDNA) and with single-stranded DNA (ssDNA) has also been studied. This character implies the potential of danthron to discriminate dsDNA and ssDNA.
Zhou
Hui
H
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
Sun
Ziyi
Z
Hoshi
Tomonori
T
Kashiwagi
Yoshitomo
Y
Anzai
Jun-Ichi
J
Li
Genxi
G
D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000880:Anthraquinones; D004277:DNA, Single-Stranded; D007364:Intercalating Agents; D004247:DNA; C004315:danthron