Molecular cloning and characterization of a porcine UL16 binding protein (ULBP)-like cDNA.
UL16 binding proteins (ULBPs) are ligands for the NK cell activating receptor NKG2D. A cDNA encoding a porcine ULBP-like protein (PULBP) was cloned and the predicted amino acid sequence exhibited 35-52% identity to human ULBPs. Southern blot analysis suggested that there is only one ULBP-like gene in the pig genome. Transcripts of PULBP and another potential NKG2D ligand, MIC2, were detected by RT-PCR in a wide range of tissues. Recombinant PULBP-Fc and human ULBP2-Fc fusion proteins were made and used to examine PULBP binding to porcine PBMCs and a human NK cell line (NKL cells). PULBP-Fc bound to a subpopulation of porcine PBMCs but not NKL cells. Conversely, human ULBP2-Fc did not bind to porcine PBMCs but did stably interact with NKL cells.
García-Borges
Carmen N
CN
Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, 151 Research, 4300 West 7th Street, Little Rock, Arkansas 72205, USA.
Phanavanh
Bounleut
B
Saraswati
Sarika
S
Dennis
Richard A
RA
Crew
Mark D
MD
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary