title: NMR experiments on aligned samples of membrane proteins.
abstract: NMR methods can be used to determine the structures of membrane proteins. Lipids can be chosen so that protein-containing micelles, bicelles, or bilayers are available as samples. All three types of samples can be aligned weakly or strongly, depending on their rotational correlation time. Solution NMR methods can be used with weakly aligned micelle and small bicelle samples. Solid-state NMR methods can be used with mechanically aligned bilayer and magnetically aligned bicelle samples.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: De Angelis
authors.forename: D H
authors.initials: DH
authors.lastname: Jones
authors.forename: C V
authors.initials: CV
authors.lastname: Grant
authors.forename: S H
authors.initials: SH
authors.lastname: Park
authors.forename: M F
authors.initials: MF
authors.lastname: Mesleh
authors.forename: S J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D008051:Lipid Bilayers / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017433:Protein Structure, Secondary; D013997:Time Factors
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins