Xenotransplantation of neonatal porcine islets and Sertoli cells into nonimmunosuppressed streptozotocin-induced diabetic rats.
The testis has been shown to be a privileged site for transplantation of allogenic islets in rodents, and the testicular cell aggregates are thought to confer this immunologic privilege. Recently, a group in Mexico reported transplantation of cocultured neonatal porcine islets and Sertoli cells resulting in insulin independence in nonimmunosuppressed type 1 diabetes patients. We have transplanted similar islets alone (naked islets) or cocultured islets with Sertoli cells (islet/Sertoli cells) into an omental site and other locations of nonimmunosuppressed, streptozotocin-induced diabetic male Sprague Dawley (SD) rats. Histologic examination showed viable neonatal porcine islets survived in xenografted rodents for at least 2 days, and some glucagon and inhibin stained cells appear to have survived for 4 days posttransplantation. However, histological examination did not demonstrate any difference in xenograft survival in the islets/Sertoli cells mixture compared to naked islets when transplanted into these nonimmunosuppressed diabetic rats.
Wang
D Z
DZ
Department of Surgery, National University of Singapore, Singapore. dzmwang@yahoo.com
Skinner
S
S
Elliot
R
R
Escobar
L
L
Salto-Tellez
M
M
Garkavenko
O
O
Khoo
A
A
Lee
K O
KO
Calne
R
R
Isaac
J R
JR
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018920:Coculture Techniques; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005934:Glucagon / Q000378:metabolism; D007150:Immunohistochemistry; D007265:Inhibins / Q000378:metabolism; D007515:Islets of Langerhans; D016381:Islets of Langerhans Transplantation / Q000473:pathology*; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology / Q000637:transplantation*; D013552:Swine; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000473:pathology*
D007265:Inhibins; D005934:Glucagon