Effects of chlorophyll-related compounds on hydrogen peroxide induced DNA damage within human lymphocytes.
Chlorophylls (Chl's) are the most abundant natural plant pigments. Four chlorophyll-related compounds (CRCs), including chlorophyllide a and b (Chlide a and b) and pheophorbide a and b (Pho a and b), were investigated for their antioxidative capacities to protect human lymphocyte DNA from hydrogen peroxide (H(2)O(2)) induced strand breaks and oxidative damage ex vivo. Lymphocytes exposed to H(2)O(2) at concentrations of 10 and 50 microM revealed an increased frequency of DNA single-strand breaks (ssb's; as measured by the comet assay) and also an increased level of oxidized nucleoside (as measured by 8-hydroxydeoxyguanosine, 8-OHdG). All Chl's reduced the level of DNA ssb's and 8-OHdG within human lymphocytes following exposure to 10 microM H(2)O(2). Only Pho a and b were able to decrease DNA ssb's and 8-OHdG following treatment of lymphocytes with 50 microM H(2)O(2), in a concentration-dependent fashion. It was demonstrated herein that Pho a and b were more antioxidative than others. We applied DPPH free-radical scavenge assays in vitro, and got similar results. Pho a and b had higher ability in scavenging capacities than others. We conclude that water-extract Chl's are able to enhance the ability of human lymphocytes to resist H(2)O(2)-induced oxidative damage, especially for Pho a and b.
Hsu
Ching-Yun
CY
Graduate Institute of Pharmacy and School of Nutrition and Health Science, Taipei Medical University, Taipei, Taiwan 110, ROC.
Yang
Chi-Ming
CM
Chen
Chiao-Ming
CM
Chao
Pi-Yu
PY
Hu
Shene-Pin
SP
D000975:Antioxidants / Q000494:pharmacology*; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002735:Chlorophyllides / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008214:Lymphocytes / Q000737:chemistry*
D000975:Antioxidants; D002735:Chlorophyllides; D002734:Chlorophyll; C046016:chlorophyllide b; C034388:chlorophyllide a; C032624:pheophorbide b; D006861:Hydrogen Peroxide; C032623:pheophorbide a