Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
OBJECTIVE
To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.


METHODS
A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.


RESULTS
Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.


CONCLUSIONS
SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes.
Cui
Xiao-Nan
XN
Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China.
Tang
Jian-Wu
JW
Hou
Li
L
Song
Bo
B
Li
Li
L
Liu
Ji-Wei
JW
D000818:Animals; D001201:Ascites / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000556:secondary*; D045744:Cell Line, Tumor; D015252:Deoxyribonucleases, Type II Site-Specific; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000556:secondary*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases