A fluorimetric assay for the spontaneous release of an N7-alkylguanine residue from duplex DNA.
A fluorimetric assay for monitoring depurination of the N7-alkylguanine adduct derived from the anticancer natural product leinamycin is described. This general approach could potentially provide the foundation for a high throughput assay that detects DNA-alkylating agents or a convenient continuous fluorimetric assay for base excision repair enzymes.
Shipova
Ekaterina
E
Department of Chemistry, University of Missouri-Columbia, Columbia, MO 65211, USA.
Gates
Kent S
KS
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000737:chemistry / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D007769:Lactams / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013871:Thiones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D018736:DNA Adducts; D007769:Lactams; D018942:Macrolides; D009692:Nucleic Acid Heteroduplexes; D013844:Thiazoles; D013871:Thiones; C059000:leinamycin; D006147:Guanine; D004247:DNA