[Angiotensin converting enzyme gene polymorphism and type 2 diabetic nephropathy].
OBJECTIVE
To prospectively clarify the relationship between angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and diabetic nephropathy (DN) in Type 2 diabetic patients.


METHODS
We examined 66 Type 2 diabetic patients with normal buminure. All patients suffered from diabetes mellitus for more than 5 years and matched well in age, body mass index (BMI), mean arterial pressure (MAP), fasting blood sugar (FBS), and urinary excretion of albumin (UAE). Patients were classified into 3 groups according to genotypes of ACE. ACE gene I/D polymorphism was identified by polymerase chain reaction (PCR). The patients were followed up for 5 years and their UAE, serum creatinine (Scr), and HbAlc were checked once a year.


RESULTS
Eight subjects (12%) were discontinued prematurely. At the end of the study, there were no significant differences in the clinical parameters such as BMI, MAP, FBS, HbA1c, UAE, and Scr among the 3 groups (P > 0.05), and also in DN incidence in Type 2 diabetic patients among the 3 groups (II 45.8%, ID 52.3%, and DD 46.1%, respectively, P > 0.05 ). The increased degree of UAE among the 3 groups was similar at the end-point of the study (P > 0.05).


CONCLUSIONS
The DD genotype of ACE gene may not be a clinically useful genetic marker for predicting the genesis and development of DN in Type 2 diabetic patients in Chinese. There is no association between ACE gene I/D polymorphism and the genesis and development of DN in Type 2 diabetic patients.
Liao
Lan
L
Department of Endocrinology, Xiangya Hospital, Central South University, Changsha 410008, China.
Lei
Min-xiang
MX
Chen
Hui-ling
HL
D000328:Adult; D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D017353:Gene Deletion; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D011446:Prospective Studies
D007703:Peptidyl-Dipeptidase A