Detection of oxidative stress induced by low- and high-linear energy transfer radiation in cultured human epithelial cells.
A standardized dichlorofluorescin (DCF) fluorometric assay capable of measuring radiation-induced oxidative stress was used to determine the effectiveness of protons and high-mass, high-atomic number (Z) and high-energy (HZE) particles to produce oxidative stress in vitro. Protons were found to be about equally as effective as X rays in the generation of oxidative stress in cultured cells. However, 56Fe-ion beams with energies of 1 GeV/nucleon and 5 GeV/nucleon were less effective than X rays or gamma rays in inducing dichlorofluorescin (DCFH) oxidation. The relatively lower slope values for the dose responses of HZE-particle radiation-induced DCFH oxidation indicate that the sensitivity of the DCF fluorometric assay is probably dependent on the linear energy transfer (LET) of the radiation beam.
Wan
X Steven
XS
Department of Radiation Oncology, the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Bloch
Peter
P
Ware
Jeffrey H
JH
Zhou
Zhaozong
Z
Donahue
Jeremiah J
JJ
Guan
Jun
J
Stewart
Jelena
J
Kennedy
Ann R
AR
D001940:Breast / Q000502:physiology / Q000528:radiation effects; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000502:physiology* / Q000528:radiation effects*; D005452:Fluoresceins; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D018499:Linear Energy Transfer / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage
D005452:Fluoresceins; C037631:2',7'-dichlorofluorescein