title: Peripheral nerve allografts stored in green tea polyphenol solution.
abstract: BACKGROUND
  We previously demonstrated the successful 1-month storage of peripheral nerve segments in a green tea polyphenol extract. We investigated whether this method could reduce the donor-host immune reaction associated with peripheral nerve allotransplantation.
  
  
  METHODS
  Sciatic nerve segments (20 mm long) were harvested from Dark Agouti (DA) rats, stored in polyphenol solution (1 mg/mL) for 1 month, and transplanted into recipient major histocompatibility complex-mismatched Lewis rats to bridge 15-mm-long sciatic nerve gaps (polyphenol-treated allograft group). The controls were an isograft group (nerve segments harvested from Lewis rats were immediately transplanted into Lewis rats), a polyphenol-treated isograft group (nerve segments harvested from Lewis rats were treated by polyphenol in the same method and transplanted into Lewis rats), and a fresh allograft group (nerve segments harvested from DA rats were transplanted into Lewis rats without storage). To investigate the origins of the cells in the transplanted nerves, sciatic nerve segments harvested from the male DA rat donors were transplanted into female Lewis rat recipients; genomic DNA was extracted from each nerve segment and amplified by polymerase chain reaction using primers specific for the rat sex-determining region of the Y-chromosome (Sry).
  
  
  RESULTS
  Nerve regeneration in the polyphenol-treated allograft group was similar to that in the isografted group. Sry-specific bands were detected in all samples in the sex-mismatched polyphenol-treated allograft specimens despite their major histocompatibility complex incompatibility.
  
  
  CONCLUSIONS
  Storage in green tea polyphenol solution can reduce both ischemic damage to nerve tissue and donor-host immune reactions after allotransplantation.
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. kakinoki@kuhp.kyoto-u.ac.jp
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ikeguchi
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kakinoki
authors.forename: Taiichi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Suong-Hyu
authors.initials: SH
authors.lastname: Hyon
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation / Q000276:immunology / Q000379:methods; D004594:Electrophysiology; D005419:Flavonoids / Q000494:pharmacology*; D023281:Genomics; D006085:Graft Survival / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D009416:Nerve Regeneration; D010636:Phenols / Q000494:pharmacology*; D010641:Phenotype; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D012996:Solutions / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D012996:Solutions; D013662:Tea