Cell immobilization using self-assembled synthetic oligopeptide and its application to biological toxicity detection using surface plasmon resonance.
The immobilized cell using self-assembled synthetic oligopeptide was applied to the biological toxicity detection of environmental pollutant. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of Escherichia coli O157:H7 on gold (Au) substrate. Layer formation and immobilization of E. coli O157:H7 were investigated with surface plasmon resonance (SPR) and atomic force microscopy (AFM). Experimental results showed that the thin film of cysteine-terminated synthetic oligopeptide was successfully fabricated and it could be applied for the immobilization of E. coli O157:H7. The attached living cell was exposed to toxic chemical such as phenol, which induced the change of SPR angle. As the exposed concentration of phenol was increased, the change of plasmon resonance angle was increased, which indicates the decrease of cell viability. The detection limit based on SPR was determined as 5 ppm. The proposed cell immobilization method using self-assembly technique can be applied to construct the cell microarray for the diagnosis, drug detection, and on-site monitoring.
Choi
Jeong-Woo
JW
Department of Chemical and Biomolecular Engineering, and Program of Integrated Biotechnology, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, South Korea. jwchoi@ccs.sogang.ac.kr
Park
Kwang-Won
KW
Lee
Doo-Bong
DB
Lee
Woochang
W
Lee
Won Hong
WH
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized / Q000502:physiology; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019453:Escherichia coli O157 / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D019800:Phenol / Q000032:analysis* / Q000494:pharmacology; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D009842:Oligopeptides; D019800:Phenol