title: The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients.
abstract: To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community- (CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p<0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community.
authors.affiliation: Department of Medical Microbiology, University Hospital Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands.
authors.forename: Ruud H
authors.initials: RH
authors.lastname: Deurenberg
authors.forename: Rutger F
authors.initials: RF
authors.lastname: Nieuwenhuis
authors.forename: Christel
authors.initials: C
authors.lastname: Driessen
authors.forename: Nancy
authors.initials: N
authors.lastname: London
authors.forename: Frank R
authors.initials: FR
authors.lastname: Stassen
authors.forename: Frank H
authors.initials: FH
authors.lastname: van Tiel
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Stobberingh
authors.forename: Cornelis
authors.initials: C
authors.lastname: Vink
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D017714:Community-Acquired Infections / Q000453:epidemiology* / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000235:genetics* / Q000378:metabolism; D014890:Granulomatosis with Polyangiitis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D018089:Superantigens / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal