In vitro function of platelet concentrates prepared after filtration of whole blood or buffy coat pools.
METHODS
Data on the quality of platelet concentrates (PC) produced by the buffy coat method and stored beyond 5 days in plasma are limited. We therefore evaluated the quality of PCs prepared by leucocyte depletion of whole blood (Terumo WBSP, n = 10) or a buffy coat pool (Pall Autostop, n = 10), and stored for 7 days in plasma by assessing platelet parameters and markers of platelet activation.


RESULTS
In both types of PC, levels of glucose decreased during storage but were not totally depleted (> 11 mM on day 7). In contrast, lactate levels increased on storage and was consistently < 20 mM throughout, with pH maintained at > 6.8 in all units. Hypotonic shock response scores were > 47% in all units at day 7. On day 1, markers of platelet activation were significantly higher in WBSP PC, but by day 7 were similar for percentage CD63+ and CD62P + (40%) with levels of platelet microparticles and annexin V binding two-fold higher in WBSP. The expression of CD61 did not alter during storage and the percentage of platelets expressing CD42b was > 88% in all units on day 7. RANTES (Regulated on activation, normal, T-cell expressed and secreted) and TGFbeta released from platelets by day 7 was < 800 ng/ml and 90 ng/ml, respectively. C3a(desarg) increased throughout storage in both types of PC, but without a commensurate increase in the terminal complex SC5b-9 or activation of factor XII.


CONCLUSIONS
Our data indicates that the in vitro characteristics of PCs prepared using these methods is maintained over storage for 7 days in plasma and is not associated with significant deterioration of platelet function. ONE SENTENCE SUMMARY: In vitro function of platelet concentrates prepared by either filtration of whole blood, or pooled buffy coats.
Turner
C P
CP
Components Development Laboratory, National Blood Service, Brentwood, Essex, UK. Craig.Turner@nbs.nhs.uk
Sutherland
J
J
Wadhwa
M
M
Dilger
P
P
Cardigan
R
R
D000704:Analysis of Variance; D017304:Annexin A5 / Q000737:chemistry; D015703:Antigens, CD / Q000096:biosynthesis; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019007:P-Selectin / Q000096:biosynthesis; D015539:Platelet Activation; D010976:Platelet Count; D010979:Platelet Function Tests; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D017713:Platelet Transfusion / Q000379:methods; D011485:Protein Binding; D013048:Specimen Handling; D060149:Tetraspanin 30; D013997:Time Factors
D017304:Annexin A5; D015703:Antigens, CD; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30