Excision of nucleoside analogs from DNA by p53 protein, a potential cellular mechanism of resistance to inhibitors of human immunodeficiency virus type 1 reverse transcriptase.
We investigated the ability of p53 in cytoplasm to excise nucleoside analogs (NAs). A decrease in incorporation of NAs by human immunodeficiency virus type 1 reverse transcriptase and their excision from DNA by p53, provided by the cytoplasmic fraction of LCC2 cells, suggest that p53 in cytoplasm may act as an external proofreader for NA incorporation.
Bakhanashvili
Mary
M
Infectious Diseases Unit, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel. bakhanus@yahoo.com
Novitsky
Elena
E
Rubinstein
Ethan
E
Levy
Itzchak
I
Rahav
Galia
G
D019380:Anti-HIV Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D024882:Drug Resistance, Viral; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D019380:Anti-HIV Agents; D004279:DNA, Viral; D009705:Nucleosides; D018894:Reverse Transcriptase Inhibitors; D016159:Tumor Suppressor Protein p53; D054303:HIV Reverse Transcriptase