Peroral infectivity of Toxoplasma gondii in bile and feces of interferon-gamma knockout mice.
Toxoplasama gondii appeared in the bile and feces of interferon-gamma knockout (GKO) but not wild type mice on days 7-8 after peroral infection with T. gondii cysts of Fukaya strain. Both tachyzoite-specific SAG1 and bradyzoite-specific T.g. HSP30 mRNAs were detected in the bile and feces of GKO mice. Tachyzoites converted to bradyzoites by culturing in the bile. By feeding uninfected mice with the bile and washed feces of T. gondii-infected GKO mice, T. gondii-specific antibody formation in the serum and cyst formation in the brain were observed. The novel migration route of T. gondii from liver to bile and feces in GKO mice was confirmed.
Piao
Lian-Xun
LX
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Japan.
Aosai
Fumie
F
Mun
Hye-Seong
HS
Yano
Akihiko
A
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001646:Bile / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D050890:HSP30 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D007371:Interferon-gamma / Q000172:deficiency* / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D015800:Protozoan Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000469:parasitology*
D000953:Antigens, Protozoan; D050890:HSP30 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C047168:SAG1 antigen, Toxoplasma; D007371:Interferon-gamma