Antioxidant activity of isoacteoside from Clerodendron trichotomum.
The antioxidant properties of isoacteoside, isolated from Clerodendron trichotomum (Verbenaceae), were investigated. This compound scavenged intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. This radical scavenging activity of isoacteoside protected cell viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2). Furthermore, isoacteoside reduced the apoptotic cells formation induced by H2O2, as demonstrated by the decreased number of sub-G1 hypo-diploid cells and apoptotic cell body formation. However, isoacteoside increased the activities of cellular antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Taken together, these findings suggest that isoacteoside, isolated from C. trichotomum, possesses antioxidant properties.
Chae
Sungwook
S
Natural Products Research Institute and College of Pharmacy, Seoul National University, Seoul, Korea.
Kim
Ju Sun
JS
Kang
Kyoung Ah
KA
Bu
Hyung Deuk
HD
Lee
Youngki
Y
Seo
Young Rok
YR
Hyun
Jin Won
JW
Kang
Sam Sik
SS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D002374:Catalase / Q000096:biosynthesis / Q000494:pharmacology; D018929:Cell Culture Techniques; D032409:Clerodendrum / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins; D005347:Fibroblasts; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000032:analysis; D015227:Lipid Peroxidation; D008168:Lung / Q000166:cytology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000494:pharmacology; D029648:Verbenaceae
D000975:Antioxidants; D003603:Cytotoxins; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C064683:isoacteoside; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase