title: Quantification of genetically modified soybean by quenching probe polymerase chain reaction.
abstract: Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.
authors.affiliation: Department of Chemical Engineering, Waseda University, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tani
authors.forename: Naohiro
authors.initials: N
authors.lastname: Noda
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Shinya
authors.initials: S
authors.lastname: Kurata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Akira
authors.initials: A
authors.lastname: Hirata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanagawa
mesh_terms: D015342:DNA Probes; D018744:DNA, Plant / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018744:DNA, Plant; D004274:DNA, Recombinant