[Inter-laboratory evaluation studies for establishment of notified ELISA methods for allergic substances (buckwheat)].
Inter-laboratory evaluation studies were conducted for the ELISA methods for allergic substances (buckwheat). Extracts of snack, bun and udon spiked with buckwheat standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate at 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Buckwheat Protein ELISA Kit (Buckwheat kit) and a FASTKIT Buckwheat ELISA kit (Buckwheat ELISA kit) were mostly below 10%. Mean recoveries of the buckwheat standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of buckwheat standard protein in three food extracts were in the ranges of 6.8-78.5% and 5.0-33.9% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. Reproducibility relative standard deviations of buckwheat standard protein in three food extracts were 11.9-69.5% and 16.5-34.1% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. The detection limits of both ELISA methods were 1 ng/mL in sample solutions. These results suggest that the notified ELISA methods are reliable and reproducible for the inspection of buckwheat protein levels in extracts of snack, bun and udon.
Akiyama
Hiroshi
H
National Institute of Health Sciences, Tokyo, Japan.
Nakamura
Kenjin
K
Harikai
Naoki
N
Watanabe
Hiroko
H
Iijima
Ken
K
Yamakawa
Hirohito
H
Mizuguchi
Yamato
Y
Yoshikawa
Reiji
R
Yamamoto
Miho
M
Sato
Hidetaka
H
Watai
Masatoshi
M
Arakawa
Fumihiro
F
Ogasawara
Takeshi
T
Nishihara
Rikuka
R
Kato
Hisashi
H
Yamauchi
Atsushi
A
Takahata
Yoshihisa
Y
Morimatsu
Fumiki
F
Mamegoshi
Shinichi
S
Muraoka
Shiroo
S
Honjoh
Tsutomu
T
Watanabe
Takahiro
T
Sakata
Kozue
K
Imamura
Tomoaki
T
Toyoda
Masatake
M
Matsuda
Rieko
R
Maitani
Tamio
T
D000485:Allergens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards*; D019612:Fagopyrum / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D007564:Japan; D007753:Laboratories; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000485:Allergens