title: Optimization of rosmarinic acid production by Lavandula vera MM plant cell suspension in a laboratory bioreactor.
abstract: The all-round effect of dissolved oxygen concentration, agitation speed, and temperature on the rosmarinic acid production by Lavandula vera MM cell suspension was studied in a 3-L laboratory bioreactor by means of the modified Simplex method. Polynomial regression models were elaborated for description of the process of rosmarinic acid production (Y) in the bioreactor as a consequence of the variation of the dissolved oxygen (X(1)) concentration between 10% and 50%; agitation (X(2)) between 100 and 400 rpm; and temperature (X(3)) between 22 and 30 degrees C. The optimization made it possible to establish the optimal conditions for the biosynthesis of rosmarinic acid by L. vera MM: dissolved oxygen (X(1)*), 50% of air saturation; agitation (X(2)*), 400 rpm; and temperature (X(3)*), 29.9 degrees C, where maximal yield (Y(max)) of 3489.4 mg/L of rosmarinic acid was achieved (2 times higher compared with the shake-flasks cultivation).
authors.affiliation: Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
authors.forename: Atanas I
authors.initials: AI
authors.lastname: Pavlov
authors.forename: Milen I
authors.initials: MI
authors.lastname: Georgiev
authors.forename: Ivan N
authors.initials: IN
authors.lastname: Panchev
authors.forename: Mladenka P
authors.initials: MP
authors.lastname: Ilieva
mesh_terms: D019149:Bioreactors; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides; D027523:Lavandula / Q000166:cytology / Q000378:metabolism*; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid