Genotoxic effects of eugenol, isoeugenol and safrole in the wing spot test of Drosophila melanogaster.
In the present study, the phenolic compounds eugenol, isoeugenol and safrole were investigated for genotoxicity in the wing spot test of Drosophila melanogaster. The Drosophila wing somatic mutation and recombination test (SMART) provides a rapid means to evaluate agents able to induce gene mutations and chromosome aberrations, as well as rearrangements related to mitotic recombination. We applied the SMART in its standard version with normal bioactivation and in its variant with increased cytochrome P450-dependent biotransformation capacity. Eugenol and safrole produced a positive recombinagenic response only in the improved assay, which was related to a high CYP450-dependent activation capacity. This suggests, as previously reported, the involvement of this family of enzymes in the activation of eugenol and safrole rather than in its detoxification. On the contrary, isoeugenol was clearly non-genotoxic at the same millimolar concentrations as used for eugenol in both the crosses. The responsiveness of SMART assays to recombinagenic compounds, as well as the reactive metabolites from eugenol and safrole were considered responsible for the genotoxicity observed.
Munerato
Maria Cristina
MC
Departamento de Odontologia Conservadora, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Brazil.
Sinigaglia
Marialva
M
Reguly
Maria Luíza
ML
de Andrade
Heloísa Helena Rodrigues
HH
D000818:Animals; D004331:Drosophila melanogaster; D005054:Eugenol / Q000031:analogs & derivatives* / Q000633:toxicity*; D005260:Female; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D012451:Safrole / Q000633:toxicity*; D014921:Wings, Animal / Q000187:drug effects*
D009153:Mutagens; D005054:Eugenol; C036643:isoeugenol; D012451:Safrole