title: Direct detection of Mycobacterium tuberculosis complex in pulmonary and extrapulmonary samples by BDProbeTec ET system.
abstract: BDProbeTec ET (Becton Dickinson, Sparks, Md, USA) is a fully automated walkaway system based on strand displacement amplification (SDA) technology that provides a method for the direct detection of Mycobacterium tuberculosis complex (MTBC) target sequence. The purpose of this study was to evaluate the ability of BDProbeTec ET system to detect MTBC directly from clinical specimens and compare the results with staining and culture. From February 2002 through December 2003 a total of 1521 [pulmonary (n=1329) and extrapulmonary (n=192)] specimens from 1518 patients were examined by BDProbeTec ET system for the detection of MTBC and the results were compared to those obtained by microscopy and liquid culture (BACTEC 9000 MB, Becton Dickinson). MTBC was cultivated from 65 specimens (60 pulmonary and 5 extrapulmonary) of which 43 (66.2%) (42 pulmonary and 1 extrapulmonary) were smear positive and 22 (33.8%) (18 pulmonary and 4 extrapulmonary) were smear negative. BDProbeTec ET detected MTBC in 58 (55 pulmonary and 3 extrapulmonary) of the 65 culture-positive specimens. Although the BDProbeTec ET system gave five false-negative results among the 18 smear-negative culture-positive pulmonary specimens, our results demonstrate that the BDProbeTec ET system is a reliable tool in smear-positive samples and given its technical characteristics it can be used for the rapid detection of MTBC in either pulmonary or extrapulmonary samples.
authors.affiliation: Section of Microbiology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Italy. miragliotta@midim.uniba.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Miragliotta
authors.forename: Raffaele
authors.initials: R
authors.lastname: Antonetti
authors.forename: Anna
authors.initials: A
authors.lastname: Di Taranto
authors.forename: Adriana
authors.initials: A
authors.lastname: Mosca
authors.forename: Raffaele
authors.initials: R
authors.lastname: Del Prete
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D010996:Pleural Effusion / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*; D014556:Urine / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial