Mitochondrial localization of Arabidopsis thaliana Isu Fe-S scaffold proteins.
Isu are scaffold proteins involved in iron-sulfur cluster biogenesis and playing a key role in yeast mitochondria and Escherichia coli. In this work, we have characterized the Arabidopsis thaliana Isu gene family. AtIsu1,2,3 genes encode polypeptides closely related to their bacterial and eukaryotic counterparts. AtIsu expression in a Saccharomyces cerevisiae Deltaisu1Deltanfu1 thermosensitive mutant led to the growth restoration of this strain at 37 degrees C. Using Isu-GFP fusions expressed in leaf protoplasts and immunodetection in organelle extracts, we have shown that Arabidopsis Isu proteins are located only into mitochondria, supporting the existence of an Isu-independent Fe-S assembly machinery in plant plastids.
Léon
Sébastien
S
Biochimie et Physiologie Moléculaire des Plantes, Université Montpellier-II, Institut National de la Recherche Agronomique et Ecole Nationale Supérieure d'Agronomie, F-34060 Montpellier cedex 1, France.
Touraine
Brigitte
B
Briat
Jean-François
JF
Lobréaux
Stéphane
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005816:Genetic Complementation Test; D007506:Iron-Sulfur Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins