A proteomics approach to identify changes in protein profiles in pre-cancerous colon.
The development of colon cancer is characterised by alterations in multiple genetic and epigenetic pathways in colon tissue leading ultimately to deregulation of colon epithelial cells. Early detection is an important factor in decreasing colon cancer deaths. Proteomic techniques were used to identify potential early markers in colon tissue exhibiting pre-cancerous activity that may characterise pathological changes in a chemically induced colon cancer rat model. Protein profiles were assessed in soluble and insoluble fractions prepared from distal colon of rats treated with the colonotropic carcinogen, dimethylhydrazine. Alterations in protein profiles were associated with the presence of aberrant crypt foci, hyperplasia and dysplasia, microanatomical changes, and metabolic changes in rat colon. These changes may have a potential role in the identification of pre-pathological features preceding colon tumorigenesis.
Drew
Janice E
JE
Cellular Integrity Division, Rowett Research Institute, Greenburn Road, Bucksburn, ABERDEEN, AB21 9SB Scotland, UK. jed@rri.sari.ac.uk <jed@rri.sari.ac.uk>
Rucklidge
Garry J
GJ
Duncan
Gary
G
Lufty
Awni
A
Farquharson
Andrew J
AJ
Reid
Martin D
MD
Russell
Wendy R
WR
Morrice
Philip C
PC
Arthur
John R
JR
Duthie
Garry G
GG
D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage; D002853:Chromatography, Liquid; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004127:Dimethylhydrazines / Q000008:administration & dosage; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006706:Homeostasis; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015430:Weight Gain / Q000187:drug effects
D002273:Carcinogens; D004127:Dimethylhydrazines; D009363:Neoplasm Proteins