title: Spectrophotometric determination of oxiconazole in topical lotion using methyl orange.
abstract: A spectrophotometric method is described for the determination of oxiconazole in raw material and in topical lotion. This method is based on the reaction of the oxiconazole with methyl orange in buffered aqueous solution of citric acid at pH 2.3. The chromogen, being extractable with dichloromethane, could be measured quantitatively with maximum absorption at 427 nm. The Lambert-Beer law was obeyed in the concentration range of 4.0-14.0 microg ml(-1). A prospective validation of the method showed that the method was linear (r=0.9995), precise (intra-day: CV=1.57% and inter-day: CV=1.50%) and accurate (mean recoveries: 99.69%). The results compared favourably with those of the HPLC method.
authors.affiliation: Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, CEP 97119-900-RS, Brazil.
authors.forename: Julie
authors.initials: J
authors.lastname: Milano
authors.forename: Simone Gonçalves
authors.initials: SG
authors.lastname: Cardoso
mesh_terms: D000287:Administration, Topical; D000935:Antifungal Agents / Q000032:analysis*; D001391:Azo Compounds / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000935:Antifungal Agents; D001391:Azo Compounds; D007093:Imidazoles; D007202:Indicators and Reagents; C100258:methyl orange; C022155:oxiconazole