In situ identification of hepatitis C virus RNA in muscle.
The authors examined skeletal muscle specimens from four patients with myositis and hepatitis C virus (HCV) infection. PCR analysis identified HCV RNA in muscle homogenates from two patients. In situ hybridization signals for HCV RNA were detected within muscle fibers as well as in infiltrating lymphocytes from the same patients. The results may relate to the pathomechanism of myositis in patients with HCV infection.
Ito
H
H
Department of Neurology, Kansai Medical University, 10-15, Fumizono-cho, Moriguchi, Osaka, 570-8507, Japan. itohi@takii.kmu.ac.jp
Ito
H
H
Nagano
M
M
Nakano
S
S
Shigeyoshi
Y
Y
Kusaka
H
H
D000368:Aged; D001706:Biopsy; D003402:Creatine Kinase / Q000097:blood; D004576:Electromyography; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000150:complications* / Q000175:diagnosis / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000503:physiopathology / Q000821:virology*; D009220:Myositis / Q000175:diagnosis* / Q000188:drug therapy / Q000821:virology*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D013256:Steroids / Q000008:administration & dosage
D012367:RNA, Viral; D013256:Steroids; D003402:Creatine Kinase