High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format.
Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the cryogel monolith making the monolith columns drainage protected. The application of a certain volume of liquid on top of a cryogel monolith column results in the displacement of exactly the same volume of liquid from the column. The concept of using supermacroporous gels in 96-well plate format offers new possibilities to the biotechnologist allowing separation of particulate matter, capturing of soluble material from particle containing media, and parallel assay of large number of non-clarified samples.
Galaeva
Igor Yu
IY
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden.
Dainiak
Maria B
MB
Plieva
Fatima M
FM
Hatti-Kaul
Rajni
R
Mattiasson
Bo
B
D018929:Cell Culture Techniques; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D003470:Culture Media; D012371:Robotics
D003470:Culture Media