title: Crystallization of mitochondrial respiratory complex II from chicken heart: a membrane-protein complex diffracting to 2.0 A.
abstract: A procedure is presented for preparation of diffraction-quality crystals of a vertebrate mitochondrial respiratory complex II. The crystals have the potential to diffract to at least 2.0 A with optimization of post-crystal-growth treatment and cryoprotection. This should allow determination of the structure of this important and medically relevant membrane-protein complex at near-atomic resolution and provide great detail of the mode of binding of substrates and inhibitors at the two substrate-binding sites.
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley CA 94720, USA.
authors.forename: Li Shar
authors.initials: LS
authors.lastname: Huang
authors.forename: Toni M
authors.initials: TM
authors.lastname: Borders
authors.forename: John T
authors.initials: JT
authors.lastname: Shen
authors.forename: Chung Jen
authors.initials: CJ
authors.lastname: Wang
authors.forename: Edward A
authors.initials: EA
authors.lastname: Berry
mesh_terms: D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003460:Crystallization; D018360:Crystallography, X-Ray; D042963:Electron Transport Complex II / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008929:Mitochondria, Heart / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: C478189:respiratory complex II; D042963:Electron Transport Complex II