Senescence-associated HSP60 expression in normal human skin fibroblasts.
Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.
Di Felice
Valentina
V
Dipartimento di Medicina Sperimentale, Sezione di Anatomia Umana Emerico Luna, Università di Palermo, Palermo, Italy. vdfelice@inwind.it
Ardizzone
Nella
N
Marcianò
Vito
V
Bartolotta
Tommaso
T
Cappello
Francesco
F
Farina
Felicia
F
Zummo
Giovanni
G
D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D018834:Chaperonin 60 / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D014746:Vimentin / Q000378:metabolism
D015415:Biomarkers; D018834:Chaperonin 60; D014746:Vimentin