Effect of insulin nitration by peroxynitrite on its biological activity.
Insulin may be a potential target of ONOO- during conditions involving accelerated rates of oxygen radical and nitric oxide generation in pancreatic islet beta-cells. To evaluate the effect of protein tyrosine nitration on insulin function, the porcine insulin was nitrated by ONOO-. Insulin nitration was confirmed by spectral changes, Native-PAGE, and mass spectrometry. The bioassay results show that mono-nitro-insulin affected its receptor binding and hypoglycemic capacities to a certain extent, but still hold high biological activities.
Chi
Quan
Q
Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Wang
Tielin
T
Huang
Kaixun
K
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007003:Hypoglycemia / Q000139:chemically induced; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009566:Nitrates / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry* / Q000494:pharmacology; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011972:Receptor, Insulin / Q000378:metabolism; D013057:Spectrum Analysis; D013552:Swine
D007328:Insulin; D009566:Nitrates; D030421:Peroxynitrous Acid; D011972:Receptor, Insulin