title: In-vitro study of mouse peritoneal macrophage response to virulent and avirulent strains of mycobacteria.
abstract: An immunological study of pathogenesis of tuberculosis was carried out in BALB-c mice in-vitro. Peritoneal macrophages obtained from BALB-c mice were challenged with virulent (H37Rv) and avirulent (H37Ra, BCG, M. phlei) strains of mycobacteria. Activated peritoneal macrophages showed enlargement, presence of intracellular bacteria and vacuolation. These significant changes in macrophage morphology were clearly evidenced in cells infected with virulent strains of Mycobacterium tuberculosis i.e. H37Rv while being absent in cells infected with avirulent H37Ra, BCG and M. phlei. Virulent mycobacteria (H37Rv) survive the phagocytic action of macrophages by residing inside the vacuoles. The capacity of virulent and avirulent strain to stimulate TNF-alpha production from peritoneal macrophage of BALB-c mice was also examined at different time interval i.e. 1,2,4,6 and 8th day by measuring cytolytic activity of culture supernatant against murine fibroblast cell line. The pattern of highest TNF release was in case of H37Rv and least with M. phlei as measured in culture supernatant after 1,2,4,6 and 8th day.
authors.affiliation: National Institute of Conmunicable Disease, 22-Shanmath Marg, Delhi.
authors.forename: Sanjay Singh
authors.initials: SS
authors.lastname: Negi
authors.forename: H G
authors.initials: HG
authors.lastname: Bramhne
authors.forename: Sunil
authors.initials: S
authors.lastname: Gupta
authors.forename: Gulshan
authors.initials: G
authors.lastname: Kumar
authors.forename: Usha Soren
authors.initials: US
authors.lastname: Singh
mesh_terms: D000818:Animals; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009168:Mycobacterium phlei / Q000472:pathogenicity*; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D010587:Phagocytosis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D014409:Tumor Necrosis Factor-alpha