Synthesis and evaluation of gadolinium complexes based on PAMAM as MRI contrast agents.
Diethylenetriaminepentaacetic acid (DTPA) and pyridoxamine (PM) were incorporated into the amine groups on the surface of ammonia-core poly(amidoamine) dendrimers (PAMAM, Generation 2.0-5.0) to obtain dendritic ligands. These dendritic ligands were reacted with gadolinium chloride to yield the corresponding dendritic gadolinium (Gd) complexes. The dendritic ligands and their gadolinium complexes were characterized by(1)HNMR, IR, UV and elemental analysis. Relaxivity studies showed that the dendritic gadolinium complexes possessed higher relaxation effectiveness compared with the clinically used Gd-DTPA. After administration of the dendritic gadolinium complexes (0.09 mmol kg(-1) ) to rats, magnetic resonance imaging of the liver indicated that the dendritic gadolinium complexes containing pyridoxamine groups enhanced the contrast of the MR images of the liver, provided prolonged intravascular duration and produced highly contrasted visualization of blood vessels.
Yan
Guo-Ping
GP
School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430073, P. R. China. guopyan@hotmail.com
Hu
Bin
B
Liu
Mai-Li
ML
Li
Li-Yun
LY
D000818:Animals; D003287:Contrast Media / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D005682:Gadolinium; D007089:Image Enhancement; D008099:Liver / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D004369:Pentetic Acid / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D011733:Pyridoxamine / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D003287:Contrast Media; D009942:Organometallic Compounds; D011073:Polyamines; D011733:Pyridoxamine; D004369:Pentetic Acid; D005682:Gadolinium