title: A rapid method for determining protein kinase phosphorylation specificity.
abstract: Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.
authors.affiliation: Division of Signal Transduction, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02215, USA.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Hutti
authors.forename: Emily T
authors.initials: ET
authors.lastname: Jarrell
authors.forename: James D
authors.initials: JD
authors.lastname: Chang
authors.forename: Derek W
authors.initials: DW
authors.lastname: Abbott
authors.forename: Peter
authors.initials: P
authors.lastname: Storz
authors.forename: Alex
authors.initials: A
authors.lastname: Toker
authors.forename: Lewis C
authors.initials: LC
authors.lastname: Cantley
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Turk
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D004789:Enzyme Activation; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D019151:Peptide Library; D010766:Phosphorylation; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D017346:Protein Serine-Threonine Kinases