title: Ethanol extract of Polygonum cuspidatum inhibits hepatitis B virus in a stable HBV-producing cell line.
abstract: Chronic hepatitis B virus (HBV) infection is endemic in Asia and its consequences are among the major public health problems in the world. Unfortunately, the therapeutic efficacies of present strategies are still unsatisfactory with a major concern about viral mutation. In search of effective antiviral agent, we examined the efficacy of extracts of Polygonum cuspidatum Sieb. et Zucc. (P. cuspidatum) against HBV in HepG2 2.2.15 cells by quantitative real time polymerase chain reaction. The expressions of viral antigens, HBeAg and HBsAg, were also determined by enzyme linked immunosorbent assay. The ethanol extract of P. cuspidatum could inhibit dose-dependently the production of HBV (p<0.0001) with an effective minimal dosage of 10 microg/ml. The water extract of P. cuspidatum might also inhibit the production of HBV at a higher dosage. The expression of HBsAg was significantly increased by both ethanol extract and water extract of P. cuspidatum dose-dependently (p<0.0001) and time-dependently (p<0.0001). Higher dose of water extract of P. cuspidatum (30 microg/ml) could inhibit the expression of HBeAg (p<0.05). The extract of P. cuspidatum might contain compounds that would contribute to the control of HBV infection in the future. However, its promoting effect on the expression of HBsAg and its cytotoxicity should be monitored. Further purification of the active compounds, identification and modification of their structures to improve the efficacy and decrease the cytotoxicity are required.
authors.affiliation: Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan First Road, Kaohsiung 807, Taiwan.
authors.forename: Jung-San
authors.initials: JS
authors.lastname: Chang
authors.forename: Hong-Wen
authors.initials: HW
authors.lastname: Liu
authors.forename: Kuo-Chih
authors.initials: KC
authors.lastname: Wang
authors.forename: Mei-Chun
authors.initials: MC
authors.lastname: Chen
authors.forename: Lien-Chai
authors.initials: LC
authors.lastname: Chiang
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Hua
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Lin
mesh_terms: D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects* / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D045468:Fallopia japonica / Q000737:chemistry*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D010936:Plant Extracts; D000431:Ethanol