Induction of de novo volatile terpene biosynthesis via cytosolic and plastidial pathways by methyl jasmonate in foliage of Vitis vinifera L.
The terpene biosynthesis in leaves of Vitis vinifera L. cv. Morio Muskat was studied using methyl jasmonate to induce defensive responses in vivo. The experiments demonstrated the strong activation of the de novo biosynthesis of terpenoids via the octadecanoid-signaling cascade and release of the compounds to the gas phase. Feeding experiments with [5,5-(2)H(2)]-1-deoxy-d-xylulose and [5,5-(2)H(2)]mevalonic acid lactone allowed the investigation of the dynamic allocation of resources via the mevalonic acid and 1-deoxy-d-xylulose/2-C-methyl-d-erythritol 4-phosphate (DOXP/MEP) pathway under induced conditions and after treatment with the specific inhibitors mevastatin and fosmidomycin. The experiments reveal that monoterpenes are almost exclusively synthesized via the DOXP/MEP pathway, whereas sesquiterpenes are generated via both pathways at approximately equal rates. The biosynthesis of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene was not affected by mevastatine or fosmidomycin.
Hampel
Daniela
D
Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany.
Mosandl
Armin
A
Wüst
Matthias
M
D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D054883:Oxylipins; D018515:Plant Leaves / Q000378:metabolism* / Q000648:ultrastructure; D018087:Plastids / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*; D027843:Vitis / Q000187:drug effects / Q000378:metabolism*; D014835:Volatilization
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D013729:Terpenes; C072239:methyl jasmonate