"In vitro" protection of DNA from Fenton reaction by plant polyphenol verbascoside.
The protection effect of verbascoside (Ver) against Fenton reaction on plasmid pBR322 DNA was studied using agarose gel electrophoresis and UV-visible spectroscopy. The pBR322 plasmid DNA is damaged by hydroxyl radical (OH*) generated from the Fenton reaction with H2O2 and Fe(II) or Fe(III). This DNA damage is characterized by the diminution of supercoiled DNA forms or by the increase of relaxed or linear DNA forms after oxidative attack. The UV spectrum study showed that verbascoside can form complexes with Fe(II) or Fe(III), and the complexation can be reversed by the addition of EDTA. The formation constants of verbascoside-Fe complexes were estimated as 10(21.03) and 10(31.94) M(-2) for Fe(II) and Fe(III) respectively. The inhibition of Fenton reaction by verbascoside could be partially explained by the sequestration of Fe ions.
Zhao
Chenyang
C
ITODYS-CNRS UMR 7086, University Paris 7-Denis Diderot, 1, rue Guy de la Brosse, 75005 Paris, France.
Dodin
Guy
G
Yuan
Chenshan
C
Chen
Haifeng
H
Zheng
Rongliang
R
Jia
Zhongjian
Z
Fan
Bo-Tao
BT
D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D005960:Glucosides / Q000378:metabolism / Q000494:pharmacology*; D017665:Hydroxyl Radical; D007501:Iron / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism / Q000494:pharmacology*; D059808:Polyphenols
D000975:Antioxidants; D005960:Glucosides; D017665:Hydroxyl Radical; D007501:Iron; D010636:Phenols; C058956:acteoside; D059808:Polyphenols