title: Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS.
abstract: The recombinant nucleocapsid protein (rNP) of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) was expressed in a baculovirus system. The purified SARS-CoV rNP was used as an antigen for detection of SARS-CoV antibodies in IgG enzyme-linked immunosorbent assay (ELISA). The ELISA was evaluated in comparison with neutralizing antibody assay and the authentic SARS-CoV antigen-based IgG ELISA. Two-hundred and seventy-six serum samples were collected from health care workers in a hospital in which a nosocomial SARS outbreak took place and used for evaluation. The SARS-CoV rNP-based IgG ELISA has 92% of sensitivity and specificity compared with the neutralizing antibody assay and 94% sensitivity and specificity compared with the authentic SARS-CoV antigen-based IgG ELISA. The results suggest that the newly developed SARS-CoV rNP-based IgG ELISA is a valuable tool for the diagnosis and seroepidemiological study of SARS. The SARS-CoV rNP-based IgG ELISA has an advantage over the conventional IgG ELISA in that the antigen can be prepared by laboratory workers without the risk of infection.
authors.affiliation: Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama 208-0011, Tokyo, Japan. msaijo@nih.go.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Toshio
authors.initials: T
authors.lastname: Ogino
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Taguchi
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Tsugunori
authors.initials: T
authors.lastname: Notomi
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kanda
authors.forename: Harumi
authors.initials: H
authors.lastname: Minekawa
authors.forename: Shutoku
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Hoang Thuy
authors.initials: HT
authors.lastname: Long
authors.forename: Nguyen Thi Hong
authors.initials: NT
authors.lastname: Hanh
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002522:Chlorocebus aethiops; D000086462:Coronavirus Nucleocapsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D019590:Nucleocapsid Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D007074:Immunoglobulin G; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins