Biosensors based on binding-modulated donor-acceptor distances.
The promising recognition characteristics exhibited by biomolecules have caused significant interest in biomolecule-based sensor strategies. Here we review several emerging biosensor designs that use modulated electron or energy transfer to a bio-specific ligand as the signaling mechanism. The efficiencies of both electron transfer and energy transfer are strongly dependent on donor-acceptor distance. When coupled with the large conformational changes sometimes associated with biomolecular recognition, these distance-dependent processes provide a robust means for generating optical and electronic signals.
Fan
Chunhai
C
Division of Nanobiology and Nanomedicine, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China. fchh@sinap.ac.cn
Plaxco
Kevin W
KW
Heeger
Alan J
AJ
D015374:Biosensing Techniques / Q000379:methods / Q000639:trends*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods / Q000639:trends; D045663:Quantum Dots