[Study on thermostability and photo-isomerization of trans-resveratrol by high performance liquid chromatography and liquid chromatography-electrospray ionization-mass spectrometry].
The thermostability and photo-isomerization reaction mechanism of trans-resveratrol were studied by using high performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry ( LC-ESI-MS). A reversed-phase Hypersil-ODS column was used with the mobile phase consisting of 60/40 methanol-water, with addition of 0.05% trifluoroacetic acid for HPLC, and 0.1% formic acid and 5 mmol/L ammonium formate for LC-ESI-MS, at a flow rate of 0.5 mL/min. Diode array detection was set at 300 nm and the injection volume was 20 or 10 microL. Mass spectrometric conditions were in the negative mode. The results of thermostability test under 4, 20 and 40 degrees C indicated that the concentration of trans-resveratrol, after storage for 600 h, was decreased slightly under 4 degrees C, decreased by 5% under 20 degrees C, and 10% under 40 degrees C. In addition, the photo-isomerization reaction of trans- and cis-resveratrol under 254 nm and 365 nm UV irradiation was investigated by LC-ESI-MS, showing that the isomerization is based on p-status, and that some secondary reactions are radical induced.
Liu
Yuping
Y
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Wen
Dawei
D
Chen
Zheng
Z
Liao
Yiping
Y
Liu
Huwei
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006358:Hot Temperature; D007536:Isomerism; D013058:Mass Spectrometry; D000077185:Resveratrol; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry*
D013267:Stilbenes; D000077185:Resveratrol