title: Vaccination against fowl cholera with acapsular Pasteurella multocida A:1.
abstract: We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
authors.affiliation: Australian Research Council Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Vic. 3800, Australia.
authors.forename: Jing Yeng
authors.initials: JY
authors.lastname: Chung
authors.forename: Ian
authors.initials: I
authors.lastname: Wilkie
authors.forename: John D
authors.initials: JD
authors.lastname: Boyce
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000502:physiology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D051379:Mice; D008807:Mice, Inbred BALB C; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines