A new renal mitochondrial carrier, KMCP1, is up-regulated during tubular cell regeneration and induction of antioxidant enzymes.
The mitochondrial carrier family transports a variety of metabolites across the inner mitochondrial membrane. We identified and cloned a new member of this family, KMCP1 (kidney mitochondrial carrier protein-1), that is highly homologous to the previously identified protein BMCP1 (brain mitochondrial carrier protein-1). Western blotting and in situ experiments showed that this carrier is expressed predominantly within the kidney cortex in the proximal and distal tubules. KMCP1 was increased during fasting and during the regenerative phase of glycerol-induced renal failure. We show that both situations are associated with transiently increased expression of superoxide-generating enzymes, followed by increased mitochondrial metabolism and antioxidant defenses. Given that KMCP1 expression occurs simultaneously with these latter events, we propose that KMCP1 is involved in situations in which mitochondrial metabolism is increased, in particular when the cellular redox balance tends toward a pro-oxidant status.
Haguenauer
Anne
A
CNRS UPR9078, Faculté Necker-Enfants Malades, 156 Rue de Vaugirard, 75730 Paris Cedex 15, France.
Raimbault
Serge
S
Masscheleyn
Sandrine
S
Gonzalez-Barroso
Maria del Mar
Mdel M
Criscuolo
Francois
F
Plamondon
Julie
J
Miroux
Bruno
B
Ricquier
Daniel
D
Richard
Denis
D
Bouillaud
Frederic
F
Pecqueur
Claire
C
D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism; D047468:Immunoprecipitation; D007473:Ion Channels; D007668:Kidney / Q000378:metabolism*; D007684:Kidney Tubules / Q000502:physiology*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000502:physiology*; D008969:Molecular Sequence Data; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012038:Regeneration; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D000071256:Uncoupling Protein 1; D015854:Up-Regulation
D000975:Antioxidants; D002352:Carrier Proteins; D018076:DNA, Complementary; D007473:Ion Channels; C502452:KMCP1 protein, mouse; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D016877:Oxidants; D000071256:Uncoupling Protein 1; D005973:Glutamine; D012313:RNA; D013482:Superoxide Dismutase; D005990:Glycerol; D010100:Oxygen