Molecular epidemiology of Salmonella enterica serovar Enteritidis strains by pulsed-field gel electrophoresis isolated in the Slovak Republic.
A total 44 isolates of S. enterica serovar Enteritidis (S. Enteritidis) belonged to three different phage types (PTs) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (PFGE). Thirty and three strains were from two outbreaks (central and southern regions of the Slovak Republic PTs 13a, 25) and 11 isolates were sporadic isolates (PT9a). These isolates showed two different patterns in PFGE with XbaI digestion. Strains of PT13a generated PFP A and isolates of PT25 showed uniform PFP B. Nine sporadic isolates of PT9a belonged to PFP A and two isolates to PFP B. The PFPs A and B were differed by only two bands. The distribution of XbaI profiles did not corresponded with PTs. We conclude that the close genetic similarity observed between epidemiologically unrelated and outbreak-related isolates of S. Enteritidis suggest clonal relationship of these isolates.
Majtánová
L
L
Slovak Medical University, Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Szaboová
M
M
Majtán
V
V
D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017720:Molecular Epidemiology; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics*; D018154:Slovakia / Q000453:epidemiology