Regulation of apoptotic pathways in bovine gamma/delta T cells.
T lymphocytes bearing gamma/delta TCRs are a major population of T cells in neonatal calves and discrete subsets of gamma/delta T cells display tissue-specific accumulation and responsiveness to infection. To enhance our understanding of the immunobiology of gamma/delta T cells, we characterized the gene expression profile of circulating bovine gamma/delta T cells following stimulation with recombinant human IL-2 and ConA. Statistical analysis of microarray data identified 108 genes with significantly altered expression, including four genes associated with apoptosis. Real-time reverse transcription-PCR (RT-PCR) analysis of 15 genes related to apoptotic pathways showed that both the Fas-mediated and the mitochondrial apoptotic pathways were repressed in circulating bovine gamma/delta T cells in response to mitogen activation, indicating that stimulated peripheral bovine gamma/delta T cells are resistant to activation-induced apoptosis.
Deng
Mingqi
M
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Liu
Jin
J
Pelak
Colleen N
CN
Lancto
Cheryl A
CA
Abrahamsen
Mitchell S
MS
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D003208:Concanavalin A / Q000276:immunology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D012313:RNA / Q000737:chemistry / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*
D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D003208:Concanavalin A; D012313:RNA