title: Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle.
abstract: Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67deltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67deltaSS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 microg per dose, which is a major improvement over previous recombinant p67 products.
authors.affiliation: Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Kaba
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Musoke
authors.forename: Dick
authors.initials: D
authors.lastname: Schaap
authors.forename: Theo
authors.initials: T
authors.lastname: Schetters
authors.forename: John
authors.initials: J
authors.lastname: Rowlands
authors.forename: Arno N
authors.initials: AN
authors.lastname: Vermeulen
authors.forename: Vishvanath
authors.initials: V
authors.lastname: Nene
authors.forename: Just M
authors.initials: JM
authors.lastname: Vlak
authors.forename: Monique M
authors.initials: MM
authors.lastname: van Oers
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D007114:Immunization; D008297:Male; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000302:isolation & purification; D013801:Theileriasis / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C114309:p67 antigen, Theileria