title: Chiral stationary phase based on a biostable L-RNA aptamer.
abstract: An immobilized anti-L-arginine d-RNA aptamer, used as a target-specific chiral stationary phase (CSP), was found to be very quickly degraded by RNases under usual chromatographic utilization and storage. To overcome this severe limitation for a practical use, a CSP based on the L-RNA aptamer, that is, the mirror image of the D-RNA aptamer, was created. It was shown that this mirror-image approach was a very simple and powerful strategy to develop a highly stable stationary phase due to the intrinsic insensitivity of l-RNA to the RNase degradation. In addition, such an approach allowed one to reverse the enantiomer elution order relative to that obtained with the corresponding d-RNA CSP.
authors.affiliation: Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Joseph Fourier, UFR de Pharmacie de Grenoble, Avenue de Verdun, 38240 Meylan, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Brumbt
authors.forename: Corinne
authors.initials: C
authors.lastname: Ravelet
authors.forename: Catherine
authors.initials: C
authors.lastname: Grosset
authors.forename: Anne
authors.initials: A
authors.lastname: Ravel
authors.forename: Annick
authors.initials: A
authors.lastname: Villet
authors.forename: Eric
authors.initials: E
authors.lastname: Peyrin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism*; D001120:Arginine / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D020871:RNA Stability; D012260:Ribonucleases / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D052157:Aptamers, Nucleotide; D001426:Bacterial Proteins; C066114:biotin-streptavidin complex; D001710:Biotin; D001120:Arginine; D012260:Ribonucleases