Mucosal targeting of allergen-loaded microspheres by Aleuria aurantia lectin.
Murine intestinal M-cells express alpha-L-fucose residues. We constructed alpha-L-fucose-targeting particles for oral immunotherapy of IgE-mediated allergy. Poly(D,L-lactic-co-glycolic acid)-microspheres were loaded with birch pollen allergens, and functionalised with the alpha-L-fucose specific Aleuria aurantia lectin (AAL). The AAL-microspheres had a size of 1-3 microm, protected the entrapped allergens from gastric degradation and released 46.6+/-1.3% allergen over 21 days in vitro. Oral gavages of AAL-particles to naive BALB/c mice induced birch pollen-specific IgG2a, but not IgG1 antibodies. We conclude that targeting allergens to alpha-L-fucose-receptor bearing cells using AAL-microspheres induces specific Th1-antibody responses possibly counteracting Th2-dominated allergy, and therefore provides a potentially useful formulation for oral immunotherapy.
Roth-Walter
Franziska
F
Department of Pathophysiology, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
Schöll
Isabella
I
Untersmayr
Eva
E
Ellinger
Adolf
A
Boltz-Nitulescu
George
G
Scheiner
Otto
O
Gabor
Franz
F
Jensen-Jarolim
Erika
E
D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D029662:Betula / Q000276:immunology; D018938:Caco-2 Cells; D003888:Desensitization, Immunologic; D020895:Enterocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019344:Lactic Acid / Q000008:administration & dosage*; D037102:Lectins / Q000008:administration & dosage / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011058:Pollen / Q000276:immunology*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*
D000485:Allergens; D007074:Immunoglobulin G; D037102:Lectins; D011108:Polymers; C064549:lectin, Aleuria aurantia; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid