Effect of Chlamydia pneumoniae infection and hyperlipidaemia on the expression of PPARgamma, P50 and c-Fos in aortic endothelial cells in C57bL/6J mice.
OBJECTIVE
To investigate the effect of C. pneumoniae infection and/or hyperlipidaemia on the expression of peroxisome proliferator-activated receptor gamma (PPARgamma), nuclear factor-kappa B (NF-kappaB) and activated protein-1 (AP-1) in aortic endothelial cells in C57BL/6J mice.


RESULTS
Forty-eight, 8-week-old female C57BL/6J mice were divided into four groups:A, B, C and D (each twelve mice). Group A (as blank control) and B were fed a regular diet. Group C and D were fed an atherogenic diet (consisting of 15% fat, 2.5% cholesterol and 0.5% sodium cholate). Group B and D were infected with C. pneumoniae. Fourteen weeks later, the expression of PPARgamma, P50 (subunit of NF-kappaB) and c-Fos (subunit of AP-1) was determined by indirect immunofluorescence in the aortic endothelial cells. Slides of aortic sinus were prepared by cryosection, and stained with Sudan IV for examination of atherosclerotic plaque. The score of atherosclerotic plaque was determined by microscopy. The score of atherosclerotic plaque in group B was not increased, while it was significantly higher in groups C and D (P < 0.01), still the score in group D was higher than in group C (P < 0.01). The expression of PPARgamma, NF-kappaB and AP-1 in endothelial cells in aortic sinus was upregulated in group B, C and D, in comparison with group A (P < 0.05). There was no significant difference among groups B, C and D.


CONCLUSIONS
The expression of PPARgamma, NF-kappaB and AP-1 was upregulated in the endothelial cells in mice infected with C. pneumoniae and/or fed with an atherogenic diet. An atherogenic diet or this diet combined with C. pneumoniae infection accelerated the process of atherosclerosis. The diet infected with C. pneumoniae alone would not accelerate this process. PPARgamma might play an anti-atherosclerotic role in this process.
Huang
Bingsheng
B
Department of Cardiology, First Affiliated Hospital of Zhongshan University, Guangzhou, China.
Dong
Yugang
Y
Mai
Weiyi
W
Li
Yongqiang
Y
D000818:Animals; D001707:Biopsy, Needle; D002690:Chlamydia Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D016993:Chlamydophila pneumoniae; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016762:Genes, fos; D006949:Hyperlipidemias / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D047495:PPAR gamma / Q000032:analysis / Q000378:metabolism*; D011336:Probability; D011897:Random Allocation; D012680:Sensitivity and Specificity; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000378:metabolism*
D018836:Inflammation Mediators; D016328:NF-kappa B; D047495:PPAR gamma; D018808:Transcription Factor AP-1