title: Separation and quantification of beer carbohydrates by high-performance liquid chromatography with evaporative light scattering detection.
abstract: An HPLC method with an evaporative light scattering detector was optimized and validated for quantification of carbohydrates in beer. The chromatographic separation was achieved using a Spherisorb NH2, 5 microm chromatographic column and gradient elution with acetonitrile/water. The determinations were performed in the linear range of 0.05-5.0 g/L for fructose, 0.05-5.0 g/L for glucose, 0.05-15.0 g/L for maltose, 0.05-10.0 g/L for maltotriose, and 0.05-5.0 g/L for maltotetraose. The detection limits were 0.005 g/L for fructose, 0.008 g/L for glucose, and 0.01 g/L for maltose, maltotriose, and maltotetraose. The reliability of the method in terms of precision and accuracy was evaluated in three beer matrices, low alcohol beer, 6% alcohol beer, and beer made with part of adjuncts (4.5% alcohol). Relative standard deviations (RSDs) ranged between 1.59 and 5.95% (n = 10), and recoveries ranged between 94 and 98.4%.
authors.affiliation: Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Brazil. luciana.nogueira@ig.com.br
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Nogueira
authors.forename: Filipe
authors.initials: F
authors.lastname: Silva
authors.forename: Isabel M P L V O
authors.initials: IM
authors.lastname: Ferreira
authors.forename: L C
authors.initials: LC
authors.lastname: Trugo
mesh_terms: D001515:Beer / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D002241:Carbohydrates