[Function of auxin-binding protein gene during cucumber fruit development].
A cDNA fragment that encodes auxin-binding protein 1 was amplified by, reverse polymerase chain reaction from ovary of cucumber. Its expression signals were weak in the ovary of 1 d before anthesis, while got strong in 2, 4 and 6 d after pollination. Among the unpollinated ovary of 2 d after anthesis, those that got enlarged had strong expression signals; the others that were wilting had weak signals. This indicated that auxin-binding protein 1 gene possibly play a role in cucumber fruit development. When auxin-binding protein 1 gene of Arabidopsis was transformed into cucumber, the parthenocarpic rate of transgenic plants was 31.7%, higher than the control. This result showed that the sensibility to auxin was increased in transgenic plants.
Bai
Ji Gang
JG
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Wang
Xiu Juan
XJ
Yin
Qian Xun
QX
Xu
Yu Zhen
YZ
Zhao
Rui Xue
RX
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D018553:Cucumis sativus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D007210:Indoleacetic Acids; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1