Genetically engineered mouse melanoma and mouse Lewis lung carcinoma models.
Melanoma B16-F10 cells and Lewis lung carcinoma LL/2 cells were engineered with a bacterial gene -- chloramphenicol acetyl transferase (CAT) -- by establishing stable transductants. Expression of CAT in both cell types did not alter the ability of these cells to grow into tumors when injected subcutaneously into mice. In addition, the measurement of CAT levels in the lung using a simple ELISA assay revealed a close correlation with direct counting of metastatic nodules. Thus, the CAT-expressing cells will likely have wide ranging applications to quantify tumor metastasis especially in situations where visual counting is difficult. The availability of genetically labeled mouse B16-F10 melanoma and Lewis lung carcinoma cell lines will facilitate future studies of the mechanism and progression of cancer and the discovery of new therapies.
Galcheva-Gargova
Zoya
Z
Momenta Pharmaceuticals Inc., Cambridge, MA 02142, USA. zoya@momentapharma.com
Qi
Yi Wei
YW
Geisler
Sarah
S
Zhidkova
Natalia
N
Nugent
Matthew A
MA
Venkataraman
Ganesh
G
D000818:Animals; D001419:Bacteria / Q000235:genetics; D018827:Carcinoma, Lewis Lung / Q000473:pathology*; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D005818:Genetic Engineering; D008545:Melanoma / Q000235:genetics* / Q000662:veterinary*; D051379:Mice; D012878:Skin Neoplasms / Q000235:genetics* / Q000662:veterinary*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D015500:Chloramphenicol O-Acetyltransferase