Liver repopulation after hepatocellular transplantation: integration and interaction of transplanted hepatocytes in the host.
The mechanisms of donor hepatocyte integration into recipient liver are not fully understood. We investigated mechanisms of both the integration and interaction of transplanted hepatocytes with host liver cells as well as the repopulation of the host organ following intraportal transplantation. Mature hepatocytes were injected into the portal vein of dipeptidylpeptidase IV (DPPIV)-deficient rats pretreated with retrorsine and subjected to 30% partial hepatectomy to ensure selective donor growth. The degree of integration and proliferation was studied by colocalizing transplanted cells (DPPIV positive) with connexin 32, MMP-2, and OX-43 (multilayer immunofluorescence imaging). FACS analysis was established to assess the extent of repopulation quantitatively. Transplanted hepatocytes reached the distal portal spaces and sinusoids within 1 h after injection. A small proportion of cells succeeded in traversing the endothelial barrier through mechanical disruption in both locations. Transplanted hepatocytes lost their membrane-bound gap junctions (connexin 32) during this process. Successful integration of the donor cells required up to 5 days, heralded by gap junction reconstitution and the specific basolateral membrane expression of DPPIV. MMP-2 degraded the extracellular matrix in close proximity to donor cells, providing space for cell division. FACS analysis revealed that more than 37% of the liver was repopulated by cells derived from donors at 2 months after transplantation. Our data demonstrate a high degree of donor cell repopulation of the host organ and provide valuable insight into the specific mechanisms of donor cell integration. Connexin 32 expression in transplanted hepatocytes may serve as an indicator of their effective incorporation and communication within the recipient liver. FACS analysis reveals an accurate method to determine quantitatively the extent of liver repopulation.
Koenig
Sarah
S
Department of General Surgery, Georg-August-University Goettingen, 37099 Germany. sarah.koenig@medizin.uni-goettingen.de
Stoesser
Claudia
C
Krause
Petra
P
Becker
Heinz
H
Markus
Peter M
PM
D000818:Animals; D030801:Animals, Genetically Modified; D002450:Cell Communication / Q000502:physiology*; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology; D017690:Cell Transplantation; D017630:Connexins / Q000032:analysis; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D017629:Gap Junctions / Q000737:chemistry / Q000378:metabolism; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism / Q000637:transplantation*; D008099:Liver / Q000166:cytology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D000097002:Gap Junction beta-1 Protein
D017630:Connexins; D018819:Dipeptidyl Peptidase 4; D020778:Matrix Metalloproteinase 2; D000097002:Gap Junction beta-1 Protein