Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography--fluorescence detection.
An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for pre-column derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6% for FB1 and 95.3% for AP1. The limit of quantification (LOQ) in swine liver was 75 microg/kg for FB1 and 42 microg/kg for AP1.
Pagliuca
Giampiero
G
Department of Veterinary Public Health and Animal Pathology, Food Hygiene and Technology Section, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, I 40064, Italy. pagliuca@vet.unibo.it
Zironi
Elisa
E
Ceccolini
Alberto
A
Matera
Riccardo
R
Serrazanetti
Gian Paolo
GP
Piva
Andrea
A
D000818:Animals; D002264:Carboxylic Acids / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013552:Swine
D002264:Carboxylic Acids; D037341:Fumonisins; C110744:aminopentol; C056933:fumonisin B1