Expression and antigenicity of virus-like particles of norovirus and their application for detection of noroviruses in stool samples.
Human noroviruses (NoVs), members of the genus Norovirus in the family Caliciviridae, are the leading agents of nonbacterial acute gastroenteritis worldwide. Human NoVs are currently divided into at least two genogroups, genogroup I (GI) and genogroup II (GII), each of which contains at least 14 and 17 genotypes. To explore the genetic and antigenic relationship among NoVs, we expressed the capsid protein of four genetically distinct NoVs, the GI/3 Kashiwa645 virus, the GII/3 Sanbu809 virus, the GII/5 Ichikawa754 virus, and the GII/7 Osaka10-25 virus in baculovirus expression system. An antigen enzyme-linked immunosorbent assay (ELISA) with hyperimmune serum against the four recombinant capsid proteins and characterized previously three capsid proteins derived from GI/1, GI/4, and GII/12 was developed to detect the NoVs antigen in stools. The antigen ELISA was highly specific to the homotypic strains, allowing assignment of a strain to a Norovirus genetic cluster within a genogroup.
Kamata
Kunio
K
Technical Marketing Department, Denka-Seiken Co., Ltd., Gosen, Niigata, Japan.
Shinozaki
Kuniko
K
Okada
Mineyuki
M
Seto
Yoshiyuki
Y
Kobayashi
Shinichi
S
Sakae
Kenji
K
Oseto
Mitsuaki
M
Natori
Katsuro
K
Shirato-Horikoshi
Haruko
H
Katayama
Kazuhiko
K
Tanaka
Tomoyuki
T
Takeda
Naokazu
N
Taniguchi
Koki
K
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D017250:Caliciviridae Infections / Q000175:diagnosis; D036022:Capsid Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis; D006801:Humans; D029322:Norovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis
D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins