title: Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with electrochemical detection.
abstract: We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotarnine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotarnine were less than 4.8%. The method developed here was better than the method reported previously.
authors.affiliation: Department of Pharmacy, Kitasato University Hospital, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan. kokubun@nc.kitasato-u.ac.jp
authors.forename: Hideya
authors.initials: H
authors.lastname: Kokubun
authors.forename: Makiko
authors.initials: M
authors.lastname: Ouki
authors.forename: Motohiro
authors.initials: M
authors.lastname: Matoba
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kubo
authors.forename: Sumio
authors.initials: S
authors.lastname: Hoka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yago
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000097:blood*; D010098:Oxycodone / Q000097:blood*; D044005:Tetrahydroisoquinolines / Q000097:blood*
chemical_list: D000701:Analgesics, Opioid; D044005:Tetrahydroisoquinolines; D010098:Oxycodone; C024594:hydrocotarnine