[Inter-laboratory evaluation studies for establishment of notified ELISA methods for allergic substances (peanuts)].
Inter-laboratory evaluation studies were conducted for ELISA methods for allergic substances (peanuts). Extracts of biscuit, sauce, chocolate and butter spiked with peanut standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Peanut Protein ELISA Kit (Peanut kit) and a FASTKIT Peanut ELISA kit (Peanut ELISA kit) were mostly below 10%. Mean recoveries of the peanut standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of peanut standard protein in four food extracts were in the ranges of 15.2-49.7% and 3.0-28.3% for the Peanut kit and the Peanut ELISA kit, respectively. Reproducibility relative standard deviations of peanut standard protein in four food extracts were 23.5-44.4%, 9.6-28.4% for the Peanut kit and the Peanut ELISA kit, respectively. The detection limits of both ELISA methods were 2-2.5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of peanut protein levels in extracts of biscuit, sauce, chocolate and butter.
Akiyama
Hiroshi
H
National Institute of Health Sciences, Tokyo, Japan.
Nakamura
Kenjin
K
Harikai
Naoki
N
Watanabe
Hiroko
H
Iijima
Ken
K
Yamakawa
Hirohito
H
Mizuguchi
Yamato
Y
Yoshikawa
Reiji
R
Yamamoto
Miho
M
Sato
Hidetaka
H
Watai
Masatoshi
M
Arakawa
Fumihiro
F
Ogasawara
Takeshi
T
Nishihara
Rikuka
R
Kato
Hisashi
H
Yamauchi
Atsushi
A
Takahata
Yoshihisa
Y
Morimatsu
Fumiki
F
Mamegoshi
Shinichi
S
Muraoka
Shiroo
S
Honjoh
Tsutomu
T
Watanabe
Takahiro
T
Sakata
Kozue
K
Imamura
Tomoaki
T
Toyoda
Masatake
M
Matsuda
Rieko
R
Maitani
Tamio
T
D000485:Allergens / Q000032:analysis*; D010367:Arachis / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards*; D005504:Food Analysis / Q000379:methods*; D007564:Japan; D007753:Laboratories; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000485:Allergens