Gas chromatographic method for the analysis of allelopathic natural products in rye (Secale cereale L.).
Accurate and reproducible methods for the analysis of plant allelochemicals are a requirement for the study of chemical interactions between plants. This paper describes a method for sample preparation and quantitative analysis of the allelopathic chemical content of rye (Secale cereale L.) using gas chromatography (GC). Sample preparation consists of extraction of freeze-dried rye vegetative tissue with aqueous ethanol followed by partitioning of the allelochemicals into ethyl acetate, evaporation, and derivatization using the trimethylsilylating reagent N-methyl-N-trimethylsilyltrifluoroacetamide. GC analysis of the silylated mixture was performed using flame ionization detection. This method permits analysis of all known rye allelopathic agents including 2,4-dihydroxy-1,4-benzoxazin-3-one, its corresponding glucoside, 2-benzoxazolinone, beta-hydroxybutyric acid, and beta-phenyllactic acid. Identities of all compounds were confirmed by GC/MS analysis.
Finney
M M
MM
Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA.
Danehower
D A
DA
Burton
J D
JD
D020155:3-Hydroxybutyric Acid / Q000032:analysis; D048588:Benzoxazines; D001583:Benzoxazoles / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D005960:Glucosides / Q000032:analysis; D006540:Herbicides / Q000032:analysis*; D007773:Lactates / Q000032:analysis; D010078:Oxazines / Q000032:analysis*; D012434:Secale / Q000737:chemistry*
D048588:Benzoxazines; D001583:Benzoxazoles; D005960:Glucosides; D006540:Herbicides; D007773:Lactates; D010078:Oxazines; C017648:3-phenyllactic acid; C017057:benzoxazolone; C104846:2,4-dihydroxy-1,4-benzoxazin-3-one; D020155:3-Hydroxybutyric Acid