title: Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles.
abstract: Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions.
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte MG, Brazil. mtmrtf@gwumc.edu
authors.forename: R T
authors.initials: RT
authors.lastname: Fujiwara
authors.forename: L L
authors.initials: LL
authors.lastname: Bueno
authors.forename: A M
authors.initials: AM
authors.lastname: Vale
authors.forename: J C
authors.initials: JC
authors.lastname: Franca-Silva
authors.forename: R T
authors.initials: RT
authors.lastname: da Costa
authors.forename: J da Silva
authors.initials: Jda S
authors.lastname: Quetz
authors.forename: G L
authors.initials: GL
authors.lastname: Machado-Coelho
authors.forename: A B
authors.initials: AB
authors.lastname: Reis
authors.forename: O A
authors.initials: OA
authors.lastname: Martins Filho
authors.forename: O
authors.initials: O
authors.lastname: Genaro
authors.forename: E
authors.initials: E
authors.lastname: Nascimento
authors.forename: W
authors.initials: W
authors.lastname: Mayrink
mesh_terms: D000818:Animals; D001938:Brazil; D004285:Dogs / Q000097:blood*; D005434:Flow Cytometry / Q000662:veterinary*; D007958:Leukocyte Count / Q000379:methods / Q000662:veterinary*; D012016:Reference Values