title: The inhibition of diacylglycerol acyltransferase by terpenoids from Youngia koidzumiana.
abstract: The EtOAc extract of Youngia koidzumiana significantly inhibited the diacylglycerol acyltransferase (DGAT) from rat liver microsomes. Bioactivity-guided fractionation led to the isolation of nine compounds, the structures of which were established using physicochemical and spectral data. Of the isolated compounds, oleanolic acid (2), methyl ursolate (7) and corosolic aicd (8) inhibited DGAT, with IC50 values of 31.7, 26.4, and 44.3 microM, respectively. However, sesquiterpenoids showed only weak inhibitory effects toward DGAT.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Nguyen Tien
authors.initials: NT
authors.lastname: Dat
authors.forename: Xing Fu
authors.initials: XF
authors.lastname: Cai
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D051048:Diacylglycerol O-Acyltransferase; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013729:Terpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013729:Terpenes; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase