Molecular mechanisms by which rRNA mutations confer resistance to clindamycin.
The mechanisms by which rRNA mutations confer clindamycin resistance were examined in Mycobacterium smegmatis strains containing homogeneous populations of ribosomes with base substitutions at nucleotides A2058 and A2059. Computer graphic predictions based on structural studies correlate with the resistance phenotypes for six of seven strains with unique rRNA mutations.
Poehlsgaard
Jacob
J
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. jacobp@bmb.sdu.dk
Pfister
Peter
P
Böttger
Erik C
EC
Douthwaite
Stephen
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics
D000900:Anti-Bacterial Agents; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D002981:Clindamycin