title: Incidence and distribution of hybrid goblet cells in complete type intestinal metaplasia of the stomach.
abstract: Previous reports suggest that hybrid goblet cells (HGCs) sharing both gastric and intestinal mucin phenotypes are rarely observed in complete intestinal metaplasia (cIM) of the stomach. However, we have made a different observation. Thus, we compared the incidence and distribution of HGCs within the tubules of gastric cIM and the duodenum in order to define the significance of HGCs. Fifteen antral sections and 16 fundic sections from tissue with cIM and gastric cancer, as well as 19 sections from duodenal tissue with cancer of the Papilla of Vater, were stained for human gastric mucin (HGM), Con A, MUC2, CD10, and Ki-67. Multivariate analysis showed that antral location, a distance of 5mm or less from the tumor margin, and the presence of underlying pyloric glands were significant predictive factors for tubules containing >50% HGCs as part of their goblet cell population. The incidence of tubules with HGCs differed significantly in tissue samples from the antrum, body and duodenum. HGCs did not stain for Ki-67 and were not surrounded by gastric foveolar-type epithelium within the tubules of cIM foci. These findings indicate that alterations in the proportion of HGCs may occur under some circumstances, and that HGCs are not precursors to gastric foveolar-type cells in the stomach and duodenum.
authors.affiliation: Division of Molecular and Diagnostic Pathology, Graduate School of Medical and Dental Science, Niigata University, 1-757 Asahimachi-dori Ichibancho, Niigata-city 951-8510, Japan. raihara@med.gunma-u.ac.jp
authors.forename: Ryuusuke
authors.initials: R
authors.lastname: Aihara
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ajioka
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Watanabe
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Shiroshita
authors.forename: Kohei
authors.initials: K
authors.lastname: Akazawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
mesh_terms: D000368:Aged; D002452:Cell Count; D004386:Duodenum / Q000473:pathology; D020397:Goblet Cells / Q000473:pathology*; D006801:Humans; D006822:Hybrid Cells / Q000473:pathology*; D007422:Intestines / Q000473:pathology*; D008679:Metaplasia; D008875:Middle Aged; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*