[A study on the chondrogenesis of the compound of alginate gelatin and bone marrow stromal cells in vivo].
OBJECTIVE
To investigate the chondrogenisis by alginate gelatin and rats' bone marrow stromal cells (BMSCs) chondrogenicly induced in vitro.


METHODS
Thirty-two male adult SD rats were assigned randomly to experimental and control groups. In experimental group, bone marrow was obtained from the right tibias of all the rats. After expanding and culturing 3 passages, induced BMSCs by chondrogenic culture medium for 10 days. Suspended induced cells in alginate gelatin, and injected the complex into the hypodermic tissue of the backs of rats autogenously. In control group only alginate gelatins were injected. The grafts were taken out for examinations 4 and 8 weeks after the operations.


RESULTS
Considerable cartilage appeared in experimental group 8 weeks after operations. Regular HE staining and alcian blue staining showed a great deal of cartilage holding chondrocyte masses surrounded by abundant matrix. Alginate gelatin decompounded obviously, and the rest distributed among newly formed cartilage. No cartilage appeared in control group all through.


CONCLUSIONS
BMSCs and alginate gelatin have a beautiful future in cartilage tissue engineering.
Liu
Lei
L
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu, China.
Chen
Rurt-liang
RL
Tian
Wei-dong
WD
Yan
Zheng-bin
ZB
Chen
Xi-zhe
XZ
Li
Sheng-wei
SW
Wang
Tao
T
D000464:Alginates; D000818:Animals; D020219:Chondrogenesis; D005780:Gelatin; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008297:Male; D059630:Mesenchymal Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin