title: A2 gene of Old World cutaneous Leishmania is a single highly conserved functional gene.
abstract: BACKGROUND
  Leishmaniases are among the most proteiform parasitic infections in humans ranging from unapparent to cutaneous, mucocutaneous or visceral diseases. The various clinical issues depend on complex and still poorly understood mechanisms where both host and parasite factors are interacting. Among the candidate factors of parasite virulence are the A2 genes, a family of multiple genes that are developmentally expressed in species of the Leishmania donovani group responsible for visceral diseases (VL). By contrast, in L. major determining cutaneous infections (CL) we showed that A2 genes are present in a truncated form only. Furthermore, the A2 genomic sequences of L. major were considered subsequently to represent non-expressed pseudogenes 1. Consequently, it was suggested that the structural and functional properties of A2 genes could play a role in the differential tropism of CL and VL leishmanias. On this basis, it was of importance to determine whether the observed structural/functional particularities of the L. major A2 genes were shared by other CL Leishmania, therefore representing a proper characteristic of CL A2 genes as opposed to those of VL isolates.
  
  
  METHODS
  In the present study we amplified by PCR and sequenced the A2 genes from genomic DNA and from clonal libraries of the four Old World CL species comparatively to a clonal population of L. infantum VL parasites. Using RT-PCR we also amplified and sequenced A2 mRNA transcripts from L. major.
  
  
  RESULTS
  A unique A2 sequence was identified in Old World cutaneous Leishmania by sequencing. The shared sequence was highly conserved among the various CL strains and species analysed, showing a single polymorphism C/G at position 58. The CL A2 gene was found to be functionally transcribed at both parasite stages.
  
  
  CONCLUSIONS
  The present study shows that cutaneous strains of leishmania share a conserved functional A2 gene. As opposed to the multiple A2 genes described in VL isolates, the CL A2 gene is unique, lacking most of the nucleotide repeats that constitute the variable region at the 5'end of the VL A2 sequences. As the variable region of the VL A2 gene has been shown to correspond to a portion of the protein which is highly immunogenic, the present results support the hypothesis of a possible role of the A2 gene in the differential tropism of CL and VL leishmania parasites.
authors.affiliation: Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, UFR Lariboisière, Université Paris VII, France. yves.garin@sls.aphp.fr
authors.forename: Yves J F
authors.initials: YJ
authors.lastname: Garin
authors.forename: Pascale
authors.initials: P
authors.lastname: Meneceur
authors.forename: Francine
authors.initials: F
authors.lastname: Pratlong
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Dedet
authors.forename: Francis
authors.initials: F
authors.lastname: Derouin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lorenzo
mesh_terms: D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D007891:Leishmania / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D037521:Virulence Factors / Q000235:genetics
chemical_list: D015800:Protozoan Proteins; D037521:Virulence Factors