title: A proteomics approach to identify changes in protein profiles in pre-cancerous colon.
abstract: The development of colon cancer is characterised by alterations in multiple genetic and epigenetic pathways in colon tissue leading ultimately to deregulation of colon epithelial cells. Early detection is an important factor in decreasing colon cancer deaths. Proteomic techniques were used to identify potential early markers in colon tissue exhibiting pre-cancerous activity that may characterise pathological changes in a chemically induced colon cancer rat model. Protein profiles were assessed in soluble and insoluble fractions prepared from distal colon of rats treated with the colonotropic carcinogen, dimethylhydrazine. Alterations in protein profiles were associated with the presence of aberrant crypt foci, hyperplasia and dysplasia, microanatomical changes, and metabolic changes in rat colon. These changes may have a potential role in the identification of pre-pathological features preceding colon tumorigenesis.
authors.affiliation: Cellular Integrity Division, Rowett Research Institute, Greenburn Road, Bucksburn, ABERDEEN, AB21 9SB Scotland, UK. jed@rri.sari.ac.uk <jed@rri.sari.ac.uk>
authors.forename: Janice E
authors.initials: JE
authors.lastname: Drew
authors.forename: Garry J
authors.initials: GJ
authors.lastname: Rucklidge
authors.forename: Gary
authors.initials: G
authors.lastname: Duncan
authors.forename: Awni
authors.initials: A
authors.lastname: Lufty
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Farquharson
authors.forename: Martin D
authors.initials: MD
authors.lastname: Reid
authors.forename: Wendy R
authors.initials: WR
authors.lastname: Russell
authors.forename: Philip C
authors.initials: PC
authors.lastname: Morrice
authors.forename: John R
authors.initials: JR
authors.lastname: Arthur
authors.forename: Garry G
authors.initials: GG
authors.lastname: Duthie
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage; D002853:Chromatography, Liquid; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004127:Dimethylhydrazines / Q000008:administration & dosage; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006706:Homeostasis; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015430:Weight Gain / Q000187:drug effects
chemical_list: D002273:Carcinogens; D004127:Dimethylhydrazines; D009363:Neoplasm Proteins