Towards a polymeric binding mimic for cytochrome CYP2D6.
A series of fluorescent molecularly imprinted polymers has been prepared with a view to generating material capable of mimicking the binding characteristics of the metabolically important cytochrome isoform CYP2D6. Such polymers would have the possibility to form the sensing element in a high-throughput assay for the prediction of CYP2D6 affinity. The imprinted polymers possessed binding-dependent fluorescence. They re-bound their templates and various cross-reactivities were encountered for test compound/drug recognition. One polymer in particular exhibited a rational discrimination amongst the related synthetic templates and was reasonably successful in recognising CYP2D6 substrates from a drug panel.
Rathbone
Daniel L
DL
School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK. D.L.Rathbone@aston.ac.uk
Ali
Aisha
A
Antonaki
Polyxeni
P
Cheek
Sarah
S
D001665:Binding Sites; D040761:Biomimetic Materials / Q000032:analysis / Q000737:chemistry*; D003198:Computer Simulation; D019389:Cytochrome P-450 CYP2D6 / Q000032:analysis* / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D008958:Models, Molecular; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations; D011108:Polymers; D019389:Cytochrome P-450 CYP2D6