Dynein promotes achiasmate segregation in Schizosaccharomyces pombe.
Most organisms use crossovers (chiasmata) to maintain physical connections between homologous chromosomes that ensure their proper segregation at the first meiotic division. The fission yeast Schizosaccharomyces pombe has a residual ability to segregate homologous chromosomes in the absence of meiotic recombination (achiasmate segregation). Using cytologically tagged chromosomes, we established a role for the microtubule motor dynein in meiotic chromosome segregation. Dhc1, the motor subunit of dynein, is required for chromosome segregation in both the presence and the absence of recombination. Dlc1, a member of the Tctex-1 dynein light-chain family, preferentially affects the segregation of achiasmate chromosomes. Dlc1 is the first identified protein, outside of Drosophila, that preferentially affects achiasmate chromosome segregation. We discuss possible roles of the dynein motor in this process.
Davis
Luther
L
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
Smith
Gerald R
GR
D000782:Aneuploidy; D002842:Chromatids / Q000378:metabolism; D020090:Chromosome Segregation; D004398:Dyneins / Q000737:chemistry / Q000502:physiology*; D005656:Fungal Proteins / Q000378:metabolism; D005838:Genotype; D008540:Meiosis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000378:metabolism / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008941:Spindle Apparatus; D056427:t-Complex Genome Region; D000099257:Meiotic Recombination Protein SPO11
D004398:Dyneins; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C456270:rec12 protein, S pombe; D000099257:Meiotic Recombination Protein SPO11; C505433:Dhc1 protein, S pombe; C505434:Dlc1 protein, S pombe