title: [Neuroprotection by resveratrol against cytotoxicity of oxidized low density lipoprotein in PC12 cells].
abstract: OBJECTIVE
  To investigate neuroprotection of resveratrol against cytotoxicity of oxidized low density lipoprotein (oxLDL) in PC12 cells.
  
  
  METHODS
  PC12 cells were pretreated with resveratrol for one hour as a neuron model and then exposed to oxLDL at varied concentrations. Effects of resveratrol on cell viability, permeability of cell membrane, cell nucleus and activity of caspase-3 were evaluated with MTT assay, lactic acid dehydrogenase (LDH) release assay, DNA fragmentation (TUNEL) assay and assay for caspase-3 activity (caspase assay).
  
  
  RESULTS
  Cell viability, LDH release rate, percentage of cells with positive TUNEL and activity of caspase-3 were (62 +/- 3)%, (23 +/- 3)%, (26 +/- 5)% and (0.811 +/- 0.049) mol.min(-1).microg(-1), vs (84 +/- 7)%, (13 +/- 4)%, (12 +/- 4)% and (0.553 +/- 0.048) mol.min(-1).microg(-1) in PC12 cells treated with 10 mg/L oxLDL vs in those treated with 10 mg/L oxLDL plus 50 micromol/L resveratrol, respectively, with statistically significant difference.
  
  
  CONCLUSIONS
  Resveratrol could attenuate cytotoxicity induced by oxLDL in PC12 cells with neuroprotection.
authors.affiliation: Department of Neurology, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000633:toxicity*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D008077:Lipoproteins, LDL; D018696:Neuroprotective Agents; D013267:Stilbenes; C099252:oxidized low density lipoprotein; D000077185:Resveratrol