Microbial production of L-ascorbic acid from D-sorbitol, L-sorbose, L-gulose, and L-sorbosone by Ketogulonicigenium vulgare DSM 4025.
Ketogulonicigenium vulgare DSM 4025, known as a 2-keto-L-gulonic acid producing strain from L-sorbose via L-sorbosone, surprisingly produced L-ascorbic acid from D-sorbitol, L-sorbose, L-gulose, and L-sorbosone as the substrate under a growing or resting condition. As the best result, K. vulgare DSM 4025 produced 1.37 g per liter of L-AA from 5.00 g per liter of L-sorbosone during 4 h incubation time at 30 degrees C under the resting cell condition having 5.70 g per liter of wet cells. The precursor of L-AA formation from D-sorbitol and L-sorbose, except for L-gulose, was thought to be the putative furanose form of L-sorbosone. This is the first time it is reported that bacteria can produce vitamin C via L-sorbosone.
Sugisawa
Teruhide
T
Biotechnology R&D, DSM Nutritional Products Ltd., Basel, Switzerland. teruhide.sugisawa@dsm.com
Miyazaki
Taro
T
Hoshino
Tatsuo
T
D001205:Ascorbic Acid / Q000096:biosynthesis*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism*; D013013:Sorbose / Q000031:analogs & derivatives* / Q000378:metabolism*
D006601:Hexoses; C057406:gulose; C013686:sorbosone; D013012:Sorbitol; D013013:Sorbose; D001205:Ascorbic Acid