[Extraction of ferulic acid from enzymatic hydrolysate by ion-exchange adsorption].
Anion-exchange resin, D201 was used to prepare ferulic acid from the enzymatic hydrolysate of wheat-bran and the influence of adsorption and desorption operation was researched. The optimal technology is as follows: under the room-temperature, the concentration of ferulic acid in the extraction solution is 2000 mg/L to approximately 3000 mg/L, pH and flow velocity were kept around 9.0 and 1 ml/min respectively; elution solution is composed of ethanol: water: HCl in ratio 60:36:4 (v/v), and the velocity of elution is 1 ml/min. Under optimal conditions,the recovery percent of ferulic acid from enzymatic hydrolysate exceed 97% and the purification production is in evidence.
Liu
Zili
Z
Dep. of Food Sciences and Engineering, Jinan University, Guangzhou.
Ou
Shiyi
S
Huang
Xuesong
X
Luo
Yanling
Y
Xie
Jing
J
Xu
Haiying
H
Jiang
Duxiao
D
D000327:Adsorption; D002855:Chromatography, Thin Layer / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification*; D002523:Edible Grain / Q000737:chemistry* / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007474:Ion Exchange; D013696:Temperature
D003373:Coumaric Acids; C004999:ferulic acid; D006867:Hydrolases