title: Methods to improve the yield and quality of DNA from dried and processed figs.
abstract: We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.
authors.affiliation: Department of Experimental Medicine, Section of Biotechnologies and Molecular Biology, 2nd University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Di Bernardo
authors.forename: U
authors.initials: U
authors.lastname: Galderisi
authors.forename: M
authors.initials: M
authors.lastname: Cipollaro
authors.forename: A
authors.initials: A
authors.lastname: Cascino
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D030681:Ficus / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018744:DNA, Plant