beta-Hematin (hemozoin) mediated decompostion of polyunsaturated fatty acids to 4-hydroxy-2-nonenal.
beta-Hematin is an important heme metabolite of malarial infection. Its role as an agent mediating the formation of the reactive electrophile 4-hydroxynonenal (HNE) from polyunsaturated fatty acids was investigated. In vitro formation of HNE was found to be facilitated by the presence of hemozoin in a concentration-dependent fashion. The reactivity of HNE derived from reaction with beta-hematin was confirmed through its ability to form protein adducts on myoglobin.
Miller
Crystal M
CM
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA.
Carney
Clare Kenny
CK
Schrimpe
Alexandra C
AC
Wright
David W
DW
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006420:Hemeproteins / Q000032:analysis* / Q000096:biosynthesis; D008288:Malaria / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry; D010963:Plasmodium falciparum / Q000378:metabolism*
D000447:Aldehydes; D005231:Fatty Acids, Unsaturated; D006420:Hemeproteins; D009211:Myoglobin; C020753:hemozoin; D006418:Heme; C027576:4-hydroxy-2-nonenal