Characterization and immunomodulating activities of polysaccharide from Lentinus edodes.
The polysaccharide L-II was isolated and purified from the fruiting body of Lentinus edodes, which consisted of d-glucopyranose and had the molecular weight of 2.03 x 10(5) Da. We evaluated the effects of the polysaccharide L-II on the cellular immune response of Sarcoma 180-bearing mice. Mice were treated with three doses of the polysaccharide L-II (1, 5, and 10 mg/kg body weight) for 10 days. Tumor weight, relative spleen and thymus weight, delayed-type hypersensitivity (DTH) response, phagocytosis of macrophage, splenocytes proliferation were studied. Concentration of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) in mice serum were measured in control and polysaccharide groups. At the dose of 1, 5 and 10 mg/kg, a significant increase (p<0.05) in relative spleen and thymus weight, DTH, phagocytosis of macrophage was observed, as well as a significant decrease in tumor formation. The concentration of TNF-alpha, IFN-gamma in serum increased significantly in the polysaccharide groups compared with the model control group, but IL-2 not. Moreover, the polysaccharide L-II could increase NO production and catalase activity in macrophages. Results of these studies demonstrated the antitumor activity of the polysaccharide L-II on mice-transplanted sarcoma 180 was mediated by immunomodulation in inducing T-cells and macrophage-dependent immune system responses.
Zheng
Ruan
R
Laboratory of Functional Food and Nutrition, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Jie
Su
S
Hanchuan
Dai
D
Moucheng
Wu
W
D000818:Animals; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006968:Hypersensitivity, Delayed; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020942:Shiitake Mushrooms / Q000737:chemistry*
D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide; D002374:Catalase