The rat brain hippocampus proteome.
The hippocampus is crucial in memory storage and retrieval and plays an important role in stress response. In humans, the CA1 area of hippocampus is one of the first brain areas to display pathology in Alzheimer's disease. A comprehensive analysis of the hippocampus proteome has not been accomplished yet. We applied proteomics technologies to construct a two-dimensional database for rat brain hippocampus proteins. Hippocampus samples from eight months old animals were analyzed by two-dimensional electrophoresis and the proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The database comprises 148 different gene products, which are in the majority enzymes, structural proteins and heat shock proteins. It also includes 39 neuron specific gene products. The database may be useful in animal model studies of neurological disorders.
Fountoulakis
Michael
M
F. Hoffmann-La Roche Ltd., Center for Medical Genomics, Basel, Switzerland. mfountoulakis@bioacademy.gr
Tsangaris
George T
GT
Maris
Antony
A
Lubec
Gert
G
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D008568:Memory / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013347:Subcellular Fractions / Q000737:chemistry
D009419:Nerve Tissue Proteins; D020543:Proteome