title: Purification of genomic DNA by short monolithic columns.
abstract: The isolation and purification of nucleic acids is essential for many procedures in molecular biology. After showing that bacterial and eukaryotic genomic DNA can be specifically bound to the CIM DEAE monolithic column, this characteristic was exploited in development of a simple and fast chromatographic procedure for isolation and purification of genomic DNA from cell lysates that does not include the usage of toxic organic solutions. The purity and the quality of the isolate as well as the duration of the procedure was similar to other chromatographic methods used today for isolation of genomic DNA, but the initial sample volume was not restricted.
authors.affiliation: Institute of Immunology Inc, Rockefellerova 10, 10000 Zagreb, Croatia. dforcic@imz.hr
authors.forename: Dubravko
authors.initials: D
authors.lastname: Forcic
authors.forename: Karmen
authors.initials: K
authors.lastname: Branovic-Cakanic
authors.forename: Jelena
authors.initials: J
authors.lastname: Ivancic
authors.forename: Renata
authors.initials: R
authors.lastname: Jug
authors.forename: Milos
authors.initials: M
authors.lastname: Barut
authors.forename: Ales
authors.initials: A
authors.lastname: Strancar
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D016678:Genome
chemical_list: D004247:DNA