Antitumour activity of Angelica archangelica leaf extract.
BACKGROUND
The purpose of this study was to examine the effect of a leaf extract from A. archangelica on the growth of Crl mouse breast cancer cells in vitro and in vivo.


METHODS
The antiproliferative activity of the extract was measured by 3H-thymidine uptake in the Crl cells in vitro. Twenty mice were injected with the Crl cells, and 11 of them were fed A. archangelica leaf extract, and the progress of the tumours was followed.


RESULTS
The leaf extract was mildly antiproliferative on the Crl cells with an EC50 of 87.6 microg/ml The antitumour activity of the extract was expressed in the mice by marked reduction in tumour growth. In the experimental animals, 9 out of 11 mice developed no or very small tumours, whereas control animals, not receiving the extract, developed significantly larger tumours (p<0.01), as estimated by Mann-Whitney U-test. The antitumour activity of the leaf extract could not be explained by the antiproliferative activity of furanocoumarins present in the extract.


CONCLUSIONS
The results demonstrate the antiproliferative activity in vitro and antitumour activity in vivo of a leaf extract from A. archangelica
Sigurdsson
Steinthor
S
Science Institute, University of Iceland, Vatnsmyrarvegur 16, Reykjavik, Iceland. sts@raunvis.hi.is
Ogmundsdottir
Helga M
HM
Hallgrimsson
Jonas
J
Gudbjarnason
Sigmundur
S
D000284:Administration, Oral; D029970:Angelica archangelica; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013936:Thymidine / Q000378:metabolism; D014185:Transplantation, Isogeneic; D014316:Tritium / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D010936:Plant Extracts; D014316:Tritium; D013936:Thymidine