title: Mouse paneth cell secretory responses to cell surface glycolipids of virulent and attenuated pathogenic bacteria.
abstract: Mouse Paneth cells respond to bacteria and bacterial cell surface antigens by discharging secretory granules into the lumen of small intestinal crypts (T. Ayabe et al., Nat. Immunol. 1:113-118, 2000). To investigate mechanisms regulating these responses, purified surface glycolipid molecules with known acyl chain modifications and attenuated properties were tested for the ability to stimulate Paneth cell secretion. The antigens included lipopolysaccharide (LPS) from wild-type and msbB-null Escherichia coli and phoP-null and phoP-constitutive Salmonella enterica serovar Typhimurium strains, as well as LPS, lipid A, and lipoteichoic acid from Pseudomonas aeruginosa and Listeria monocytogenes grown in Mg2+-limited media. Measurements of total secreted protein, secreted lysozyme, and the bactericidal peptide activities of collected secretions showed that the purified antigens elicited similar secretory responses from Paneth cells in mouse crypts ex vivo, regardless of glycolipid acyl chain modification. Despite their impaired Tlr4 pathway, Paneth cells in ex vivo C3H/HeJ mouse crypts released equivalent amounts of bactericidal peptide activity in response to purified bacterial antigens, including lipid A. Thus, mouse Paneth cells respond equivalently to purified bacterial cell envelope glycolipids, regardless of functional Tlr4, the structural properties of glycolipid acyl chains, or their association with virulence in humans.
authors.affiliation: Department of Pathology, School of Medicine, College of Health Sciences, University of California, Irvine, CA 92697-4800, USA.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanabe
authors.forename: Tokiyoshi
authors.initials: T
authors.lastname: Ayabe
authors.forename: Brian
authors.initials: B
authors.lastname: Bainbridge
authors.forename: Tina
authors.initials: T
authors.lastname: Guina
authors.forename: Robert K
authors.initials: RK
authors.lastname: Ernst
authors.forename: Richard P
authors.initials: RP
authors.lastname: Darveau
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
authors.forename: Andre J
authors.initials: AJ
authors.lastname: Ouellette
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology*; D000954:Antigens, Surface / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D006017:Glycolipids / Q000494:pharmacology*; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D019879:Paneth Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology; D013682:Teichoic Acids / Q000494:pharmacology; D051197:Toll-Like Receptor 4; D014774:Virulence; D023084:alpha-Defensins / Q000502:physiology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D006017:Glycolipids; D008050:Lipid A; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D013682:Teichoic Acids; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D023084:alpha-Defensins; C009900:lipoteichoic acid; D002118:Calcium