Potential in a single cancer cell to produce heterogeneous morphology, radiosensitivity and gene expression.
Morphologically heterogeneous colonies were formed from a cultured cell line (KYSE70) established from one human esophageal carcinoma tissue. Two subclones were separated from a single clone (clone13) of KYSE70 cells. One subclone (clone13-3G) formed mainly mounding colonies and the other (clone 13-6G) formed flat, diffusive colonies. X-irradiation stimulated the cells to dedifferentiate from the mounding state to the flat, diffusive state. Clone 13-6G cells were more radiosensitive than the other 3 cell lines. Clustering analysis for gene expression level by oligonucleotide microarray demonstrated that in the radiosensitive clone13-6G cells, expression of genes involved in cell adhesion was upregulated, but genes involved in the response to DNA damage stimulus were downregulated. The data demonstrated that a single cancer cell had the potential to produce progeny heterogeneous in terms of morphology, radiation sensitivity and gene expression, and irradiation enhanced the dedifferentiation of cancer cells.
Ban
Sadayuki
S
RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences, Anagawa, Chiba, Japan. s_ban@nirs.go.jp
Ishikawa
Ken-Ichi
K
Kawai
Seiko
S
Koyama-Saegusa
Kumiko
K
Ishikawa
Atsuko
A
Shimada
Yutaka
Y
Inazawa
Johji
J
Imai
Takashi
T
D002454:Cell Differentiation / Q000528:radiation effects*; D045744:Cell Line, Tumor; D048429:Cell Size / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects*; D003001:Cloning, Molecular / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D004938:Esophageal Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000235:genetics; D014965:X-Rays
D009363:Neoplasm Proteins