Induction of lymphokine-activated cytotoxic T lymphocytes stimulated by dendritic cells and autologous tumor from a patient with gastric cancer and their effects in vitro.
OBJECTIVE
The purpose of the study was to generate lymphokine-activated cytotoxic T lymphocytes stimulated by dendritic cells (DC) and autologous tumor from a patient with gastric cancer and to clarify their cytotoxic effects in vitro.


METHODS
DC was induced by interleukin-4 (IL-4) and granulocyte-macrophage-colony-stimulating factor (GM-CSF) from the peripheral blood mononuclear cells (PBMC). Then, PBMC was incubated with mitomycin C-treated tumor cells and DC, and following that was activated with IL-2 and anti-CD3. Induction of DC and cytotoxic T cells (CTL) were confirmed by the analyses of the cell surface antigens, killing activities, and blocking tests.


RESULTS
Induction of DC and cytotoxic T cells (CTL) was confirmed by the analyses of the cell surface antigens, killing activities, and blocking tests. In vitro study demonstrated that lymphokine-activated lymphocytes pulsed by DCs and autologous tumor contained the largest population of CTLs, the greatest production of IFN-gamma, and the greatest ATK activity.


CONCLUSIONS
Those results indicated that CTLs could be generated in vitro from a patient with gastric cancer more successfully by this method than by conventional methods, suggesting the possibility of a new immunotherapy for the treatment of gastric cancer.
Yoshikawa
Takaki
T
Department of Gastrointestinal Surgery, Kanagawa Cancer Center, Yokohama, Japan. yoshikawat@koch.jp
Yanoma
Shunsuke
S
Tsuburaya
Akira
A
Kobayashi
Osamu
O
Sairenji
Motonori
M
Motohashi
Hisahiko
H
Noguchi
Yoshikazu
Y
D017252:CD3 Complex / Q000502:physiology; D018929:Cell Culture Techniques; D003713:Dendritic Cells / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods; D007376:Interleukin-2 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*
D017252:CD3 Complex; D007376:Interleukin-2; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor