An increased level of alternative sigma factor RpoS partially suppresses drug hypersensitivity associated with inactivation of the multidrug resistance pump AcrAB in Escherichia coli.
Constitutive expression of stationary phase alternative sigma factor RpoS in Escherichia coli during the exponential phase was found to partially suppress drug sensitivity associated with an acrAB mutation that inactivates the major multidrug resistance pump, suggesting that Rpos might significantly contribute to multidrug resistance, underscoring yet another role for this important stress-related transcription factor.
Rami
Abderrahmane
A
Institut de Génétique et Microbiologie, UMR 8621, CNRS/Université Paris-Sud, Bâtiment 400, Université Paris-Sud, Orsay 91405 cedex, France.
Toutain
Christine M
CM
Jacq
Annick
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D024901:Drug Resistance, Multiple, Bacterial / Q000502:physiology*; D049071:Endopeptidase Clp / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008074:Lipoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*
C083497:AcrA protein, E coli; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; C483572:ClpP protease, E coli; D049071:Endopeptidase Clp