Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a.
Macrophage galactose-type C-type lectins (MGLs), which were recently named CD301, have 2 homologues in mice: MGL1 and MGL2. MGLs are expressed on macrophages and immature dendritic cells. The persistent presence of granulation tissue induced by a protein antigen was observed in wild-type mice but not in mice lacking an endogenous, macrophage-specific, galactose-type calcium-type lectin 1 (MGL1) in an air pouch model. The anti-MGL1 antibody suppressed the granulation tissue formation in wild-type mice. A large number of cells, present only in the pouch of MGL1-deficient mice, were not myeloid or lymphoid lineage cells and the number significantly declined after administration of interleukin 1 alpha (IL-1alpha) into the pouch of MGL1-deficient mice. Furthermore, granulation tissue was restored by this treatment and the cells obtained from the pouch of MGL1-deficient mice were incorporated into the granulation tissue when injected with IL-1alpha. Taken together, MGL1 expressed on a specific subpopulation of macrophages that secrete IL-1alpha was proposed to regulate specific cellular interactions crucial to granulation tissue formation.
Sato
Kayoko
K
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
Imai
Yasuyuki
Y
Higashi
Nobuaki
N
Kumamoto
Yosuke
Y
Onami
Thandi M
TM
Hedrick
Stephen M
SM
Irimura
Tatsuro
T
D000388:Air; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001212:Asialoglycoproteins; D001826:Body Fluids / Q000276:immunology; D004195:Disease Models, Animal; D006097:Granulation Tissue / Q000276:immunology*; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology; D037181:Lectins, C-Type / Q000235:genetics* / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D012595:Scleroderma, Systemic / Q000235:genetics / Q000276:immunology* / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D001212:Asialoglycoproteins; C064720:Clec10a protein, mouse; D007375:Interleukin-1; D037181:Lectins, C-Type; D008565:Membrane Proteins; D011994:Recombinant Proteins