Studying the biological and technical sources of variation in telomere length of individual chromosomes.
BACKGROUND
Consistent average length differences between species and chromosome arm differences within species indicate that telomere length is genetically determined. This seems to contradict an observed large variation in lengths of the same human telomere between metaphases of the same individual. We examined the extent to which the variation in the telomeres of the human X and Y chromosomes is heritable, induced, or technical in origin.


METHODS
Metaphase chromosomes were stained by fluorescence in situ hybridization with a telomere repeat-specific probe, and fluorescence intensities of the X and Y chromosomes were measured. If telomere length variation is predominantly genetically determined and a 50% probability of meiotic recombination between the pseudo-autosomal regions of Yp and Xp in the father is taken into account, one expects an equal chance that the Yp telomere of a son is derived from his father's Xp or Yp telomere. This implies that the Yp/Yq telomere ratios in fathers and sons will be identical in the absence of paternal meiotic recombination and different when recombination occurs.


RESULTS
Among five father-son pairs, four showed similar Yp/Yq ratios (P > 0.05), whereas one pair exhibited a large difference in the Yp/Yq ratio that was attributable to a significantly longer Xp than Yp telomere in the father and a presumptive meiotic exchange between X and Y during paternal meiosis. Further, the Xq telomere exhibited a generally shorter telomere length than the others.


CONCLUSIONS
The high variation in telomere length appeared to be intracellular (between sister chromatids) and, hence, technical in nature. We found no measurable induced variation in the cells studied, implying that, if induced variation exists, it is small compared with the technical variation.
de Pauw
E S D
ES
Department of Haematology, Leiden University Medical Center, Leiden, The Netherlands.
Roelofs
H
H
Zwinderman
A
A
van Houwelingen
J C
JC
Fibbe
W E
WE
de Knijff
P
P
Pearson
P L
PL
Tanke
H J
HJ
D000328:Adult; D000368:Aged; D002877:Chromosomes, Human / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D015342:DNA Probes; D014644:Genetic Variation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D016615:Telomere / Q000737:chemistry / Q000235:genetics*
D015342:DNA Probes