Effect of interleukin-1beta on the variation of adenylyl cyclase expression in rats with seizures induced by L-glutamate.
To explore the mechanism of interleukin-1beta (IL-1beta) in the onset of seizure and the effect of IL-1beta on the expression of adenylyl cyclase (AC) in rats with seizure induced by L-glutamate. Experimental rats were first injected with IL-1beta and then L-glutamate (a dose under the threshold) was injected into the right lateral ventricle. The rats were sacrificed 4 h after the onset of epileptic activity and examined for changes in behavior, immunohistochemistry and compared with those with seizure induced by L-glutamate alone. It was found that the expression of AC in hippocampal and neocortex of rats with seizure induced by IL-1beta and L-glutamate were stronger than that of control group (P<0.05), without significant difference found between the L-glutamate group and IL-1beta plus L-glutamate group in the expression of AC, the latent period and the severity of seizure. When IL-ra were given (i.c.v.) first, there was no epileptic activity and the expression of AC did not increase. There were no differences in the expression of AC of rats with IL-1ra and that of control rats. But when 2-methyl-2-(carboxycyclopropyl) glycine (MCCG) was given (i.c.v.) first, the strongest expression of AC, the shortest latent period and the the most serious seizure activities were observed. The results indicated that IL-1beta could facilitate the onset of epilepsy induced by L-glutamate through IL-1R, metabotropic glutamate receptors might work with IL-1R and the increased expression of AC might be involved in the process.
Wang
Zhen
Z
Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Qingying
Q
Zhu
Changgeng
C
D000262:Adenylyl Cyclases / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D019579:Neocortex / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000201:enzymology*
D007375:Interleukin-1; D018698:Glutamic Acid; D000262:Adenylyl Cyclases