Acute biochemical effects of La(NO3)3 on liver and kidney tissues by magic-angle spinning 1H nuclear magnetic resonance spectroscopy and pattern recognition.
High-resolution magic-angle spinning (MAS) 1H nuclear magnetic resonance (NMR) spectroscopic and pattern recognition (PR) based methods have been applied to studies on the acute biochemical effects of La(NO3)3 on rats. Male Wistar rats were treated with various doses of La(NO3)3 (2, 10, and 50 mg/kg body weight), and MAS 1H NMR spectra of their intact liver and kidney tissues were analyzed using principal components analysis to extract metabolic information. The biochemical effects of La(NO3)3 were characterized by the increase of triglyceride and bile acid and the decrease of glycogen in liver tissue, together with a slight elevation of triglyceride level in kidney tissue. The target lesion of La(NO3)3 to liver was found by MAS NMR-PR methods. This study illustrated the power of the combination of MAS 1H NMR and pattern recognition for the analysis of biochemical effects of rare earths.
Wu
Huifeng
H
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Zhang
Xiaoyu
X
Li
Xiaojing
X
Wu
Yijie
Y
Pei
Fengkui
F
D000818:Animals; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007811:Lanthanum / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008110:Liver Extracts / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010363:Pattern Recognition, Automated; D051381:Rats; D017208:Rats, Wistar
D008110:Liver Extracts; C016534:lanthanide nitrate; D007811:Lanthanum