Kinetic spectrophotometric determination of rutin by its inhibitory effect on the oxidation of amaranth by potassium periodate.
A novel kinetic spectrophotometric method is described for the determination of rutin. The method is based on the inhibitory effect of rutin on the oxidation reaction of amaranth by potassium periodate in acidic media at 100 degrees C. The linear range for the determination of rutin is 0.02 - 0.50 microg/ml, and the detection limit is 0.014 microg/ml. The method has been successfully applied to the determination of rutin in medicine of rutin tablet and traditional Chinese medicine.
Wang
Shuhao
S
College of Environmental Science and Engineering, Donghua University, Shanghai 200051, PR China. shuhaowang@sohu.com
Du
Lingyun
L
Yao
Xingjun
X
Niu
Xueli
X
Zhuang
Huisheng
H
D000548:Amaranth Dye / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D012015:Reference Standards; D012431:Rutin / Q000032:analysis*; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet; D013464:Sulfuric Acids / Q000737:chemistry
D017680:Potassium Compounds; D013464:Sulfuric Acids; D010504:Periodic Acid; D012431:Rutin; D000548:Amaranth Dye; C033158:sulfuric acid; C037192:potassium periodate