Preparation of molecularly imprinted cyclodextrin microspheres.
Molecularly imprinted cyclodextrins (MI-CDs) are prepared by cross-linking CDs in the presence of a template molecule. The binding ability of MI-CDs to the template molecule is specific; therefore, MI-CDs will prove to be useful materials. In this study, we prepared microspheres of MI-CDs (MSs-MI-CD) in a dimethylsulfoxide/poly(dimethylsiloxane) (PDMS) emulsion, using cholesterol as the template molecule. MSs-MI-CD were prepared under various conditions and were evaluated with respect to their morphology, size, and binding ability. MSs-MI-CD prepared at 65 degrees C were in an aggregated form; however, we could prepare separated and uniform MSs-MI-CD at 95 degrees C. The viscosity of PDMS influenced the size of MSs-MI-CD. The mean particle diameters of MSs-MI-CD prepared with 50 and 1000 mm(2)/s PDMS were 146 and 43 microm, respectively. The binding ability of MSs-MI-CD to cholesterol was higher than that of non-imprinted microspheres. Cholesterol imprinting also promoted the binding ability to other steroids; however, the increase in binding ability was most remarkable in the case of cholesterol, suggesting that we successfully introduced the cholesterol-specific binding ability into MSs-MI-CD. The novel MSs-MI-CD preparation method is useful and simple, and it will provide opportunities for further studies on the specific binding ability of MI-CDs.
Egawa
Y
Y
Pharmaceutical Research Laboratories, Nikken Chemicals Co. Ltd., 1-346 Kitabukuro-cho, Ohmiya-ku, Saitama-shi, Saitama, Japan. moguinukuma@yahoo.co.jp
Shimura
Y
Y
Nowatari
Y
Y
Aiba
D
D
Juni
K
K
D002784:Cholesterol / Q000378:metabolism; D003505:Cyclodextrins / Q000138:chemical synthesis* / Q000378:metabolism; D008863:Microspheres
D003505:Cyclodextrins; D002784:Cholesterol