title: [The construction of recombinant plasmid with prostate-specific membrane antigen promoter controlling reporter gene expression].
abstract: OBJECTIVE
  To study the specificity of prostate-specific membrane antigen (PSMA) promoter in controlling gene expression.
  
  
  METHODS
  PSMA promoter gene was amplified with PCR, and then the promoter gene was cloned into the vector pEGFP-1 to construct a recombinant plasmid, which was transfected into different cell lines such as LNcap, PC-3, MCF-7, A549. Green fluorescent protein (GFP) expression was observed.
  
  
  RESULTS
  The recombinant plasmid constructed, and PSMA promoter uniquely showed modulating activity in PSMA positive cell line.
  
  
  CONCLUSIONS
  PSMA promoter possesses PSMA positive cell specificity, as well as prostatic tissue specificity. PSMA promoter may have the potential for use in targeted gene therapy of prostate adenocarcinoma.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zeng
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yong-chuan
authors.initials: YC
authors.lastname: Liao
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Yu-ru
authors.initials: YR
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000628:therapy; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D043425:Glutamate Carboxypeptidase II / Q000096:biosynthesis / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000276:immunology* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000628:therapy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D012333:RNA, Messenger; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II