title: Heterologous protein production in Zygosaccharomyces bailii: physiological effects and fermentative strategies.
abstract: The optimisation and scale-up of a specific protein production process have to take into account cultivation conditions as well as cell physiology of growth and the influence of foreign protein expression on host cell metabolism. The ability of Zygosaccharomyces bailii to tolerate high sugar concentrations as well as high temperatures and acidic environments renders this "non-conventional" yeast suitable for the development of biotechnological processes like heterologous protein production. This work addresses the production of human interleukin-1beta by a recombinant Z. bailii strain. We found that the heterologous protein production causes some modifications of the Z. bailii carbon metabolism, leading to a reduced biomass yield. The other important factor is the dependence of the recombinant IL-1beta production/secretion on the growth rate. Among the cultivation strategies studied, the most appropriate in terms of production and productivity was the fed-batch mode.
authors.affiliation: Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.
authors.forename: Ileana
authors.initials: I
authors.lastname: Vigentini
authors.forename: Luca
authors.initials: L
authors.lastname: Brambilla
authors.forename: Paola
authors.initials: P
authors.lastname: Branduardi
authors.forename: Annamaria
authors.initials: A
authors.lastname: Merico
authors.forename: Danilo
authors.initials: D
authors.lastname: Porro
authors.forename: Concetta
authors.initials: C
authors.lastname: Compagno
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D020068:Zygosaccharomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D000431:Ethanol; D005947:Glucose