Epithelial differentiation of human adipose tissue-derived adult stem cells.
Adult human stem cells are employed in novel treatments and bio-artificial devices. Recent studies have identified an abundant source of stem cells termed adipose-derived adult stem (ADAS)-cells in the subcutaneous adipose tissue. Under appropriate culture conditions ADAS-cells differentiate to various cell types, including chondrocytes, adipocytes, and smooth muscle cells. Aiming at epithelial differentiation this study investigated the effect of all-trans retinoic acid (ATRA) on human ADAS-cells. ATRA-induced cytokeratin 18 expression in ADAS-cells and nearly abolished vimentin expression as shown by Western blot. In immunofluorescence, the formation of keratin fibers in ATRA-treated ADAS-cells could be observed. The percentage of ADAS-cells being able to undergo epithelial differentiation as quantified by FACS-analysis was above 80%. Inhibition of cell growth by ATRA was shown using DAPI- and MTT-assays. ATRA can differentiate ADAS-cells toward the epithelial lineage. This finding, along with a previously described neural differentiation, shows that ADAS-cells have epithelial potential.
Brzoska
Martin
M
Department of Medicine/Nephrology, Johann Wolfgang Goethe-University, Theodor-Stern-Kai 7-9, 60590 Frankfurt am Main, Germany. brzoska@em.uni-frankfurt.de <brzoska@em.uni-frankfurt.de>
Geiger
Helmut
H
Gauer
Stefan
S
Baer
Patrick
P
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
D014212:Tretinoin