Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei.
Class D beta-lactamase OXA-57 was identified in a range of isolates of Burkholderia pseudomallei and Burkholderia thailandensis. Comparative kinetic analyses of wild-type and mutant forms of B. pseudomallei OXA-57 are reported. Implications of these data for beta-lactam resistance and the proposed role of Ser-104 in beta-lactam hydrolysis are discussed.
Keith
Karen E
KE
Department of Biological Sciences, CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom.
Oyston
Petra C
PC
Crossett
Ben
B
Fairweather
Neil F
NF
Titball
Richard W
RW
Walsh
Timothy R
TR
Brown
Katherine A
KA
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D019117:Burkholderia / Q000187:drug effects / Q000201:enzymology*; D016957:Burkholderia pseudomallei / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases