[Medium effects on derivative reaction between fluorescamine and primary arlyamine].
The effects of pH, various organic solvents, surfactants, beta-CD and PVA on derivative reaction and fluorescence properties of its product between fluorescamine and p-amino phenyl sulfonic acid have been studied. The results show that the derivative reaction needs the organic solvent, many kind of organic solvents are available, but its amount should not be too large. The rate of derivative reaction was slowed and fluorescence intensity of product was decresed with increase in amount of organic solvent and precision is not good because of the fluorescence intensity is changing with delay time. Only tritonX-100 enhanced the fluorescence of product in all of the surfactants tested. Span20, Tween80 and Brij35 which same as tritonX-100 are non-ionic surfactant, and PVA, beta-CD do not exhibit sensitization. Ionic Surfactant CTAB, SDS quench the fluorescence. The sensitization of tritonX-100 is dependent on the amount of organic solvent. A competitive effect between tritonX-100 micellae and organic solvent is exhibition. In this paper, the derivative reaction between fluorescamine and p-chloroaniline is compared and studied too.
Lin
D
D
Dept of Chem, Zhangzhou Normal College, 363000 Zhangzhou.
Li
L
L