title: Analysis of diltiazem and its related substances by HPLC and HPLC/MS.
abstract: Diltiazem (DTZ) is an optically active calcium channel blocker having a benzodiazepine structure. The drug used in therapy is (+)-cis-diltiazem with configuration (2S,3S). To describe the analytical profile of DTZ different stationary phases (RP-18, RP-8, monolithic support) were tested. The best separation of DTZ from A, B, E and F was obtained using as stationary phase a RP-8 or a monolithic RP-18. The characterization of impurities was carried out using two analytical systems, HPLC and HPLC/MS.
authors.affiliation: Dipartimento Studi Farmaceutici, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, Rome 00185, Italy. mariagiovanna.quaglia@uniroma1.it
authors.forename: M G
authors.initials: MG
authors.lastname: Quaglia
authors.forename: E
authors.initials: E
authors.lastname: Donati
authors.forename: S
authors.initials: S
authors.lastname: Fanali
authors.forename: E
authors.initials: E
authors.lastname: Bossù
authors.forename: A
authors.initials: A
authors.lastname: Montinaro
authors.forename: F
authors.initials: F
authors.lastname: Buiarelli
mesh_terms: D002121:Calcium Channel Blockers / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000032:analysis* / Q000302:isolation & purification; D004340:Drug Contamination; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D013237:Stereoisomerism
chemical_list: D002121:Calcium Channel Blockers; D007202:Indicators and Reagents; D004110:Diltiazem