Quantification of genetically modified soybean by quenching probe polymerase chain reaction.
Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.
Tani
Hidenori
H
Department of Chemical Engineering, Waseda University, Shinjuku-ku, Tokyo 169-8555, Japan.
Noda
Naohiro
N
Yamada
Kazutaka
K
Kurata
Shinya
S
Tsuneda
Satoshi
S
Hirata
Akira
A
Kanagawa
Takahiro
T
D015342:DNA Probes; D018744:DNA, Plant / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000235:genetics*
D015342:DNA Probes; D018744:DNA, Plant; D004274:DNA, Recombinant