Developmentally programmed expression of AID in chicken B cells.
In mice, activation induced deaminase, AID, is expressed only in germinal center B cells. It is required for the initiation of somatic hypermutation and class switch recombination. In chickens and most mammals immunoglobulin gene rearrangement generates limited diversity and the primary immunoglobulin repertoire depends on subsequent somatic hypermutation or gene conversion. Immunoglobulin gene conversion in chickens starts in the embryonic bursa, before antigen exposure. The demonstrated requirement for AID for gene conversion in the bursal lymphoma cell line, DT40, implies developmental regulation of AID expression. To test this prediction, we examined the timing and location of AID mRNA expression. An abrupt increase in AID mRNA coincided with the onset of extensive Ig gene conversion in the bursa. Expression was also detected at earlier stages, implying either that expression of AID is not the only controlling factor for gene conversion, or that gene conversion can precede the formation of bursal follicles.
Withers
David R
DR
Immunology and Pathology Division, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK.
Davison
T Fred
TF
Young
John R
JR
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D001483:Base Sequence; D002060:Bursa of Fabricius / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002645:Chickens / Q000378:metabolism*; D003564:Cytidine Deaminase / Q000096:biosynthesis / Q000235:genetics*; D005785:Gene Conversion; D005786:Gene Expression Regulation / Q000502:physiology*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D007147:Immunoglobulin Light Chains; D012333:RNA, Messenger; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase