title: Cloning and expression of a lipoxygenase from Pseudomonas aeruginosa 42A2.
abstract: In order to produce (S) 10-monohydroxy-8E-octadecenoic acid (MHOD) from oleic acid, a full-length probable lipoxygenase cDNA from Pseudomonas aeruginosa 42A2 was cloned and expressed in Escherichia coli BL21(DE3). The recombinant protein was purified by affinity chromatography to electrophoretic homogeneity and specifically stained. Its molecular mass was 70 kDa. The activity of the rec-LOX with oleic acid was about 30% of that of the preferred substrate, linoleic acid (100%). Bacterial LOX forms a new subfamily in the lipoxygenase phylogenetic tree.
authors.affiliation: Laboratori de Microbiologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona E-08028, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Vidal-Mas
authors.forename: Montserrat
authors.initials: M
authors.lastname: Busquets
authors.forename: Angeles
authors.initials: A
authors.lastname: Manresa
mesh_terms: D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D008084:Lipoxygenase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019301:Oleic Acid / Q000378:metabolism; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019301:Oleic Acid; D019787:Linoleic Acid; D008084:Lipoxygenase