title: Identification of galanin receptor 1 on excitatory motor neurons in the guinea pig ileum.
abstract: Exogenously administered galanin inhibits cholinergic transmission to the longitudinal muscle and reduces peristaltic efficiency in the guinea pig ileum with a mechanism partially mediated by galanin receptor 1 (GAL-R1). We investigated the effect of exogenous galanin 1-16, which has high affinity for GAL-R1, on the ascending excitatory reflex of the circular muscle elicited by radial distension in isolated segments of guinea pig ileum. We used a three-compartment bath that allows dissecting the ascending pathway into the oral (site of excitatory motor neurons), intermediate (site of ascending interneurons) and caudal compartment (site of intrinsic primary afferent neurons). Galanin 1-16 (0.3-3 micromol L(-1)) applied to the oral compartment inhibited in a concentration-dependent manner the ascending excitatory reflex elicited by the wall distension in the caudal compartment. This effect was antagonized by the GAL-R1 antagonist, RWJ-57408 (1 and 10 micromol L(-1)). By contrast, galanin 1-16 was ineffective when added to the intermediate or caudal compartment up to 3 micromol L(-1). GAL-R1 immunoreactive neurons did not contain neuron-specific nuclear protein, a marker for intrinsic primary afferent neurons. These findings indicate that GAL-R1s are present on motor neurons responsible for the ascending excitatory reflex, but not on ascending interneurons and intrinsic primary afferent neurons.
authors.affiliation: CURE Digestive Diseases Research Center, Digestive Diseases Division, Veterans Administration Greater Los Angeles Healthcare System, Bldg. 115, Room 224, Vaglahs, 11301 Wilshire Blvd, Los Angeles, CA 90073, USA. lanselmi@mednet.ucla.edu
authors.forename: L
authors.initials: L
authors.lastname: Anselmi
authors.forename: E
authors.initials: E
authors.lastname: Cervio
authors.forename: S
authors.initials: S
authors.lastname: Guerrini
authors.forename: R
authors.initials: R
authors.lastname: Vicini
authors.forename: A
authors.initials: A
authors.lastname: Agazzi
authors.forename: A
authors.initials: A
authors.lastname: Dellabianca
authors.forename: J R
authors.initials: JR
authors.lastname: Reeve
authors.forename: M
authors.initials: M
authors.lastname: Tonini
authors.forename: C
authors.initials: C
authors.lastname: Sternini
mesh_terms: D000818:Animals; D019004:Galanin / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000294:innervation*; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D009046:Motor Neurons / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D009475:Neurons, Afferent / Q000378:metabolism; D009924:Organ Culture Techniques; D010446:Peptide Fragments / Q000494:pharmacology; D010528:Peristalsis / Q000502:physiology; D044091:Receptor, Galanin, Type 1 / Q000378:metabolism*; D012018:Reflex / Q000502:physiology
chemical_list: D010446:Peptide Fragments; D044091:Receptor, Galanin, Type 1; C062019:galanin (1-16); D019004:Galanin