Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery.
The emergence of a relatively new technique resulting from a combination of frontal affinity chromatography coupled with MS detection (FAC-MS) has extended the capabilities of MS in drug discovery and development. Its application in a broad range of biological systems, together with its label-free operation, relatively high throughput, ability to rank ligands and determine Kd, makes FAC-MS a universal tool enabling convenient and efficient screening in the identification of new potential drug leads. Here we will highlight FAC-MS screening studies and discuss where it can be applied in evaluating multiple protein-binding sites, protein-protein interactions and inactive proteins, and also in determining selectivity.
Slon-Usakiewicz
Jacek J
JJ
Protana, Lead Discovery and Optimization Division, 251 Attwell Drive, Toronto, ON M9W 7H4, Canada. jslon@protana.com
Ng
William
W
Dai
Jin-Rui
JR
Pasternak
Andrew
A
Redden
Peter R
PR
D001665:Binding Sites; D002846:Chromatography, Affinity; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008024:Ligands; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D011506:Proteins / Q000737:chemistry*
D008024:Ligands; D011506:Proteins