title: Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion.
abstract: Interstitial fluid pressurization plays an important role in cartilage biomechanics and is believed to be a primary mechanism of load support in synovial joints. The objective of this study was to investigate the effects of enzymatic degradation on the interstitial fluid load support mechanism of articular cartilage in unconfined compression. Thirty-seven immature bovine cartilage plugs were tested in unconfined compression before and after enzymatic digestion. The peak fluid load support decreased significantly (p < 0.0001) from 84 +/- 10% to 53 +/- 19% and from 80 +/- 10% to 46 +/- 21% after 18-hours digestion with 1.0 u/mg-wet-weight and 0.7 u/mg-wet-weight of collagenase, respectively. Treatment with 0.1 u/ml of chondroitinase ABC for 24 hours also significantly reduced the peak fluid load support from 83 +/- 12% to 48 +/- 16% (p < 0.0001). The drop in interstitial fluid load support following enzymatic treatment is believed to result from a decrease in the ratio of tensile to compressive moduli of the solid matrix.
authors.affiliation: Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Ines M
authors.initials: IM
authors.lastname: Basalo
authors.forename: Robert L
authors.initials: RL
authors.lastname: Mauck
authors.forename: Terri-Ann N
authors.initials: TA
authors.lastname: Kelly
authors.forename: Steven B
authors.initials: SB
authors.lastname: Nicoll
authors.forename: Faye H
authors.initials: FH
authors.lastname: Chen
authors.forename: Clark T
authors.initials: CT
authors.lastname: Hung
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Ateshian
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D019765:Chondroitin ABC Lyase / Q000494:pharmacology*; D017364:Collagenases / Q000494:pharmacology*; D019245:Compressive Strength / Q000187:drug effects / Q000502:physiology; D045604:Extracellular Fluid / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007719:Knee Joint / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013314:Stress, Mechanical; D016474:Weight-Bearing / Q000502:physiology*
chemical_list: D017364:Collagenases; D019765:Chondroitin ABC Lyase