Preserved synaptic vesicle recycling in hippocampal neurons in a mouse Alzheimer's disease model.
A recently described triple-transgenic mouse model (3xTg, PS1(M146V), APP(Swe), and tau(P301L)) develops a neuropathology similar to the brains of Alzheimer's disease patients including progressive deposits of plaques and tangles [Neuron 39 (2003) 409]. These mice also show age-related deficits in hippocampal synaptic plasticity that occurs before the development of plaques and tangles. Here we report unchanged synaptic vesicle recycling, as measured by FM1-43 release, in the hippocampal neurons of the 3xTg mice. Expression levels of presynaptic protein synaptophysin and of proteins involved in synaptic vesicle recycling including AP180, dynamin I, and synaptotagmin I also remain unaffected. These data suggest that the synaptic deficits observed in the 3xTg neurons may not arise from the preserved synaptic vesicle recycling.
Yao
Pamela J
PJ
Laboratory of Neurosciences, NIA/NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. yaopa@grc.nia.nih.gov <yaopa@grc.nia.nih.gov>
Bushlin
Ittai
I
Furukawa
Katsutoshi
K
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*