Detection of influenza A virus by RT-PCR and standard methods in experimental infection of Ducks.
Cloacal swabs collected from mallard ducks (Anas platyrhynchos) experimentally infected with a H7N1 avian influenza strain were examined by Reverse Transcription Polymerase Chain Reaction to detect the influenza A virus. Reverse Transcription Polymerase Chain Reaction was compared with standard methods: inoculation of embryonated chicken eggs and inoculation of three established cell lines: Newborn Swine Kidney cells, Newborn Pig Trachea cells and Madine Darby Canine Kidney cells. Reverse Transcription Polymerase Chain Reaction was performed using a set of primers based on conserved regions of the matrix and nucleoprotein genes.
Foni
Emanuela
E
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini", Sezione diagnostica di Parma, via dei Mercati 13/A, Parma, Italy. emanuela.foni@bs.izs.it
Chiapponi
Chiara
C
Lori
Daniela
D
De Marco
Maria Alessandra
MA
Delogu
Mauro
M
Raffini
Elisabetta
E
Massi
Paola
P
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002988:Cloaca / Q000821:virology; D004195:Disease Models, Animal; D004372:Ducks / Q000821:virology*; D009980:Influenza A virus / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D011201:Poultry Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014776:Virus Cultivation