title: Hepatic function in hypothermically stored porcine livers: comparison of hypothermic machine perfusion vs cold storage.
abstract: Hypothermic machine perfusion (HMP) has a potential to relieve the current donor liver crisis by providing an improved and extended preservation method. This study examined the effect of HMP on hepatocellular functions, using a prototype liver transporter capable of preserving livers for 24 hours. Livers obtained from adult farm pigs (28 to 32 kg body weight) were divided into three groups: fresh control, HMP, and simple cold storage (n = 4 each). A 4-hour normothermic reperfusion of livers was conducted to assess hepato-metabolic and cellular functions. The hepatic transport function, as indicated by canalicular excretion of indocyanine green, was improved in the HMP group than in the SCS group. The overall tissue viability, as indicated by oxygen consumption levels, was notably improved in HMP and control livers as compared to the SCS group. Higher bile production in both the preserved groups as compared to the fresh control livers could be a result of biliary edema and leakage of plasma into the canaliculus. The hepato-cellular injury, measured by ALT, release was significantly greater in the SCS group as compared to the HMP and control groups. These findings suggest that HMP could be a better method to preserve hepatic function and overall tissue viability as compared to SCS. Improved hepatic functions are indirect indicators of superior microcirculation and sinusoidal endothelial cell functions. Further studies in progress will evaluate these functions to confirm the significance of these observations.
authors.affiliation: University of North Carolina, Charlotte, NC, USA.
authors.forename: S
authors.initials: S
authors.lastname: Jain
authors.forename: C Y
authors.initials: CY
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Baicu
authors.forename: H
authors.initials: H
authors.lastname: Duncan
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: J W
authors.initials: JW
authors.lastname: Jones
authors.forename: M G
authors.initials: MG
authors.lastname: Clemens
authors.forename: J
authors.initials: J
authors.lastname: Brassil
authors.forename: M J
authors.initials: MJ
authors.lastname: Taylor
authors.forename: K G M
authors.initials: KG
authors.lastname: Brockbank
mesh_terms: D000410:Alanine Transaminase / Q000032:analysis; D000818:Animals; D001646:Bile / Q000378:metabolism; D007036:Hypothermia, Induced / Q000379:methods; D008111:Liver Function Tests; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D013552:Swine
chemical_list: D000410:Alanine Transaminase