title: Isolation of Ganjam virus from ticks collected off domestic animals around Pune, Maharashtra, India.
abstract: Studies on viruses of zoonotic importance in certain villages around Pune were undertaken between December 2000 and January 2002. A total of 1,138 adult ticks belonging to six different species were collected off domestic animals and processed for virus isolation. Six virus isolates were obtained. All six isolates were identified as Ganjam virus by Quick Complement Fixation test and reverse transcriptase-polymerase chain reaction using RNA nucleocapsid gene amplification. Five isolates were from the pools of adult Hemaphysalis intermedia ticks, and one isolate was from a pool of adult Rhipecephalus hemaphysaloides. This is the first report of isolation of Ganjam virus from Maharashtra state of India.
authors.affiliation: Division of Epidemiology National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, Maharashtra, India. mvjoshi46@rediffmail.com
authors.forename: M V
authors.initials: MV
authors.lastname: Joshi
authors.forename: G
authors.initials: G
authors.lastname: Geevarghese
authors.forename: G D
authors.initials: GD
authors.lastname: Joshi
authors.forename: Y S
authors.initials: YS
authors.lastname: Ghodke
authors.forename: D T
authors.initials: DT
authors.lastname: Mourya
authors.forename: A C
authors.initials: AC
authors.lastname: Mishra
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D002417:Cattle; D006041:Goats; D007194:India; D029281:Nairobi sheep disease virus / Q000302:isolation & purification*; D048494:Rhipicephalus / Q000821:virology; D012424:Rural Population; D012756:Sheep; D013987:Ticks / Q000821:virology*