Biocompatibility of Poly(epsilon-caprolactone) scaffold modified by chitosan--the fibroblasts proliferation in vitro.
In this study, the surface of poly(epsilon-caprolactone) (PCL) scaffold was modified by chitosan (CS) in order to enhance its cell affinity and biocompatibility. It is demonstrated by scanning electronic microscopy (SEM) that when 0.5-2.0 wt% chitosan solutions are used to modify the PCL scaffold, the amount of adhesion of the fibroblasts on the chitosan-modified PCL scaffolds dramatically increase when compared to the control after 7 days cell culture. The results indicate that the chitosan-modified PCL scaffolds are more favorable for cell proliferation by improving the scaffold biocompatibility. The improvement may be helpful for the extensive applications of PCL scaffold in heart valve and blood vessel tissue engineering.
Mei
Na
N
The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Macromolecular Science Department, Fudan University, Shanghai 200433, P.R. China.
Chen
Guang
G
Zhou
Ping
P
Chen
Xin
X
Shao
Zheng-Zhong
ZZ
Pan
Luan-Feng
LF
Wu
Chun-Gen
CG
D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan