The isolation and antioxidative effects of vitexin from Acer palmatum.
Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental factors are critical players in cellular damage and aging. In order to develop a new anti-photoaging agent, this work focused on the antioxidant effects of the extract of tinged autumnal leaves of Acer palmatum. One compound was isolated from an ethyl acetate soluble fraction of the A. palmatum extract using silica gel column chromatography. The chemical structure was identified as apigenin-8-C-beta-D-glucopyranoside, more commonly known as vitexin, by spectral analysis including LC-MS, FT-IR, UV, 1H-, and 13C-NMR. The biological activities of vitexin were investigated for the potential application of its anti-aging effects in the cosmetic field. Vitexin inhibited superoxide radicals by about 70% at a concentration of 100 microg/mL and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals by about 60% at a concentration of 100 microg/mL. Intracellular ROS scavenging activity was indicated by increases in dichlorofluorescein (DCF) fluorescence upon exposure to UVB 20 mJ/cm2 in cultured human dermal fibroblasts (HDFs) after the treatment of vitexin. The results show that oxidation of 5-(6-)chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) is inhibited by vitexin effectively and that vitexin has a potent free radical scavenging activity in UVB-irradiated HDFs. In ROS imaging using a confocal microscope we visualized DCF fluorescence in HDFs directly. In conclusion, our findings suggest that vitexin can be effectively used for the prevention of UV-induced adverse skin reactions such as free radical production and skin cell damage.
Kim
Jin Hwa
JH
R&D Center, Hanbul Cosmetics Co Ltd, 72-7 Yongsung-ri, Samsung-myun, Umsung-kun, Chungbuk, Korea.
Lee
Bum Chun
BC
Kim
Jin Hui
JH
Sim
Gwan Sub
GS
Lee
Dong Hwan
DH
Lee
Kyung Eun
KE
Yun
Yeo Pyo
YP
Pyo
Hyeong Bae
HB
D031002:Acer / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009580:Nitroblue Tetrazolium; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D047310:Apigenin; C032731:vitexin; C004931:1,1-diphenyl-2-picrylhydrazyl