Quantitative measurement of the levels of melanocortin receptor subtype 1, 2, 3 and 5 and pro-opio-melanocortin peptide gene expression in subsets of human peripheral blood leucocytes.
Levels of the melanocortin receptor (MCR) 1, 2, 3 and 5 subtypes and pro-opio-melanocortin (POMC) protein mRNA were measured by the real-time quantitative reverse transcriptase polymerase chain reaction method in CD4+ T helper (Th) cells, CD8+ T cytotoxic cells, CD19+ B cells, CD56+ natural killer (NK) cells, CD14+ monocytes and CD15+ granulocytes from healthy donors. We found high levels of all of the MC1, 2, 3 and 5R subtype mRNA in Th cells and moderate levels in NK cells, monocytes and granulocytes. POMC peptide mRNA was found in all examined leucocyte subsets, but only low levels were present in granulocytes. Our findings suggest a co-ordinating role for MCR subtypes and their naturally occurring ligands in the co-operation between innate and adaptive immunity. Moreover, our findings are compatible with earlier finding of MCR-mediated tolerance induction in Th cells.
Andersen
G N
GN
Department of Rheumatology, Umea University Hospital, Umea, Sweden. grethe_n_andersen@hotmail.com
Hägglund
M
M
Nagaeva
O
O
Frängsmyr
L
L
Petrovska
R
R
Mincheva-Nilsson
L
L
Wikberg
J E S
JE
D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D007962:Leukocytes / Q000145:classification / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics; D044103:Receptor, Melanocortin, Type 2 / Q000235:genetics; D044104:Receptor, Melanocortin, Type 3 / Q000235:genetics; D018043:Receptors, Corticotropin / Q000235:genetics; D044101:Receptors, Melanocortin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012333:RNA, Messenger; D044102:Receptor, Melanocortin, Type 1; D044103:Receptor, Melanocortin, Type 2; D044104:Receptor, Melanocortin, Type 3; D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin; C086971:melanocortin 5 receptor; D011333:Pro-Opiomelanocortin