Synthesis and circular dichroism of tetraarylporphyrin-oligonucleotide conjugates.
The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3' end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28-40 A), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400-450 nm). The porphyrin chromophores act as sensitive probes of geometrical changes in the DNA backbone and sensitively reflect the double-strand to single-strand transition. This study demonstrates the possibility of using exciton-coupled porphyrin CDs for conformational studies of DNA.
Balaz
Milan
M
Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027, USA.
Holmes
Andrea E
AE
Benedetti
Michele
M
Rodriguez
Pamela C
PC
Berova
Nina
N
Nakanishi
Koji
K
Proni
Gloria
G
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*
D009841:Oligonucleotides; D011166:Porphyrins; D004247:DNA