In vivo effects of a recombinant molt-inhibiting hormone on molt interval and hemolymph ecdysteroid level in the kuruma prawn, Marsupenaeus japonicus.
In order to determine the function of molt-inhibiting hormone (MIH) in vivo, we examined the effects of injecting of a recombinant MIH on the molt interval and hemolymph ecdysteroid level in the kuruma prawn, Marsupenaeus japonicus. The injection of recombinant MIH significantly prolonged the molt interval (9.0 +/-0.4 days in the control group, 9.5+/-0.5 days in the 2500 ng/g-body weight/injection-group, mean+/-SD), and significantly decreased the hemolymph ecdysteroid level (ratio of levels between after and before injection: 1.94+/-1.09 in the control and 1.28+/-0.39 in the 3000 ng/g-body weight/injection-group, mean+/-SD). These results conclusively show the inhibitory effects of MIH on molting in vivo.
Okumura
Takuji
T
National Research Institute of Aquaculture, Fisheries Research Agency, Nansei, Mie, Japan. takuji@affrc.go.jp
Ohira
Tsuyoshi
T
Katayama
Hidekazu
H
Nagasawa
Hiromichi
H
D000818:Animals; D026461:Ecdysteroids / Q000097:blood*; D006458:Hemolymph / Q000378:metabolism*; D007447:Invertebrate Hormones / Q000378:metabolism / Q000494:pharmacology*; D007564:Japan; D018978:Molting / Q000187:drug effects* / Q000502:physiology; D033561:Penaeidae / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D026461:Ecdysteroids; D007447:Invertebrate Hormones; D011994:Recombinant Proteins; C012445:molt-inhibiting hormone