Ethanol physical dependence is accompanied by up-regulated expression of L-type high voltage-gated calcium channel alpha1 subunits in mouse brain.
We investigated how functional changes in high voltage-gated calcium channels (HVCCs) occurred in the cerebral cortex of mouse with ethanol physical dependence. The continuous treatment of mice with ethanol vapor for 8 days significantly increased the expressions of alpha1C, alpha1D, and alpha2/delta1 subunits of L-type HVCCs in association with decreased level of beta4 subunit of L-type HVCCs, although alpha1A and alpha1B subunits of P/Q- and N-type HVCCs, respectively, showed no alterations. [(3)H]Diltiazem binding to the particulate fractions increased with increased Bmax value and no changes of Kd value. These results indicate that ethanol physical dependence accompanies the up-regulation of L- type HVCCs, which is due to increased expression of alpha1C, alpha1D, and alpha2/delta1 subunits.
Katsura
Masashi
M
Department of Pharmacology, Kawasaki Medical School, Kurashiki 701-0192, Japan.
Torigoe
Fumiko
F
Hayashida
Shinsuke
S
Honda
Takeshi
T
Tsujimura
Atsushi
A
Ohkuma
Seitaro
S
D000280:Administration, Inhalation; D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000378:metabolism*; D004110:Diltiazem / Q000378:metabolism; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051379:Mice; D009543:Nifedipine / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*; D015854:Up-Regulation
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D004791:Enzyme Inhibitors; D011720:Pyrazoles; D000431:Ethanol; D004110:Diltiazem; D009543:Nifedipine