title: Identification of an active transposon in intact rice plants.
abstract: A transposable element that is active in intact plants has been identified in rice (Oryza sativa L.). The 607-bp element itself, termed nonautonomous DNA-based active rice transposon (nDart), has no coding capacity. It was found inserted in the gene encoding Mg-protoporphyrin IX methyltransferase in a chlorophyll-deficient albino mutant isolated from backcross progeny derived from a cross between wild-type japonica varieties. The nDart has 19-bp terminal inverted repeats (TIRs) and, when mobilized, generates an 8-bp target-site duplication (TSD). At least 13 nDart elements were identified in the genome sequence of the japonica cultivar Nipponbare. Database searches identified larger elements, termed DNA-based active rice transposon (Dart) that contained one ORF for a protein that contains a region with high similarity to the hAT dimerization motif. Dart shares several features with nDart, including identical TIRs, similar subterminal sequences and the generation of an 8-bp TSD. These shared features indicate that the nonautonomous element nDart is an internal deletion derivative of the autonomous element Dart. We conclude that these active transposon systems belong to the hAT superfamily of class II transposons. Because the transposons are active in intact rice plants, they should be useful tools for tagging genes in studies of functional genomics.
authors.affiliation: Hokkaido Green-Bio Institute, Naganuma, Hokkaido, 0691317, Japan. fvgk0144@mb.infoweb.ne.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Fujino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiguchi
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Kiguchi
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003433:Crosses, Genetic; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences / Q000235:genetics
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D008780:Methyltransferases; C060234:magnesium-protoporphyrin methyltransferase