Interaction analysis of the carcinoembryonic antigen (CEA) with its monoclonal antibody immobilized on a gold surface using Fourier transform infrared reflection-absorption spectroscopy (FT-IR RAS).
A monoclonal antibody for the carcinoembryonic antigen (CEA) was immobilized on a gold chip surface covered by a self-assembled monolayer of 11-mercaptoundecanoic acid. Upon the addition of CEA, a Fourier transform infrared reflection-absorption spectroscopy (FT-IR RAS) measurement showed an increased absorption at around 1500 - 1700 cm(-1), corresponding to its amide structures. Another addition of CEA polyclonal antibody on this chip caused a further increase of the absorption in this region only after a treatment with CEA. This result shows that an antibody-fixed gold surface coupled with an FT-IR RAS measurement provides a new tool for detecting the antibody-antigen interaction.
Ohtsuka
Keiichi
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan. staketcm@mbox.nc.kyushu-u.ac.jp
Kuroki
Masahide
M
Nojima
Takahiko
T
Waki
Michinori
M
Takenaka
Shigeori
S
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002272:Carcinoembryonic Antigen / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D046208:Microchip Analytical Procedures; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D006046:Gold