Simple and simultaneous determination of the hiv-protease inhibitors amprenavir, atazanavir, indinavir, lopinavir, nelfinavir, ritonavir and saquinavir plus M8 nelfinavir metabolite and the nonnucleoside reverse transcriptase inhibitors efavirenz and nevirapine in human plasma by reversed-phase liquid chromatography.
Several studies suggest that therapeutic drug monitoring of protease inhibitors and nonnucleoside reverse transcriptase inhibitors may contribute to the clinical outcome of HIV-infected patients. Because of the growing number of antiretroviral drugs and of drug combinations than can be administered to these patients, an accurate high-performance liquid chromatographic (HPLC) method allowing the simultaneous determination of these drugs may be useful. To date, the authors present the first simultaneous HPLC determination of the new protease inhibitor atazanavir with all the others currently in use (M8 nelfinavir metabolite included) and the 2 widely used nonnucleoside reverse transcriptase inhibitors efavirenz and nevirapine. This simple HPLC method allows the analysis all these drugs at a single ultraviolet wavelength following a 1-step liquid-liquid extraction procedure. A 500-muL plasma sample was spiked with internal standard and subjected to liquid-liquid extraction using by diethyl ether at pH 10. HPLC was performed using a Symmetry Shield RP18 and gradient elution. All the drugs of interest and internal standard were detected with ultraviolet detection at 210 nm. Calibration curves were linear in the range 50-10,000 ng/mL. The observed concentrations of the quality controls at plasma concentrations ranging from 50 to 5000 ng/mL for these drugs showed that the overall accuracy varied from 92% to 104% and 92% to 106% for intraday and day-to-day analysis, respectively. No metabolites of the assayed compounds or other drugs commonly coadministered to HIV-positive patients were found to coelute with the drugs of interest or with the internal standard. This assay was developed for the purpose of therapeutic monitoring (TDM) in HIV-infected patients.
Poirier
Jean-Marie
JM
Department of Pharmacology, Saint-Antoine University Hospital, 27 rue Chaligny, 75571 Paris Cedex 12 France. Jean-Marie.Poirier@chusa.jussieu.fr
Robidou
Pascal
P
Jaillon
Patrice
P
D000480:Alkynes; D000069446:Atazanavir Sulfate; D048588:Benzoxazines; D002138:Calibration / Q000592:standards; D002219:Carbamates; D002853:Chromatography, Liquid / Q000379:methods* / Q000639:trends*; D003521:Cyclopropanes; D004355:Drug Stability; D005663:Furans; D015658:HIV Infections / Q000188:drug therapy; D017320:HIV Protease Inhibitors / Q000097:blood* / Q000145:classification* / Q000627:therapeutic use; D006801:Humans; D019469:Indinavir / Q000097:blood / Q000627:therapeutic use; D061466:Lopinavir; D019888:Nelfinavir / Q000097:blood / Q000627:therapeutic use; D019829:Nevirapine / Q000097:blood / Q000627:therapeutic use; D009842:Oligopeptides / Q000097:blood / Q000627:therapeutic use; D010078:Oxazines / Q000097:blood / Q000627:therapeutic use; D011725:Pyridines / Q000097:blood / Q000627:therapeutic use; D011744:Pyrimidinones / Q000097:blood / Q000627:therapeutic use; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood / Q000627:therapeutic use; D019438:Ritonavir / Q000097:blood / Q000627:therapeutic use; D019258:Saquinavir / Q000097:blood / Q000627:therapeutic use; D013048:Specimen Handling / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013449:Sulfonamides / Q000097:blood / Q000627:therapeutic use
D000480:Alkynes; D048588:Benzoxazines; D002219:Carbamates; D003521:Cyclopropanes; D005663:Furans; D017320:HIV Protease Inhibitors; D009842:Oligopeptides; D010078:Oxazines; D011725:Pyridines; D011744:Pyrimidinones; D018894:Reverse Transcriptase Inhibitors; D013449:Sulfonamides; D061466:Lopinavir; D000069446:Atazanavir Sulfate; C095108:amprenavir; D019469:Indinavir; D019829:Nevirapine; D019888:Nelfinavir; C098320:efavirenz; D019258:Saquinavir; D019438:Ritonavir