The effect of normovolemic hemodilution on c-Fos protein immunoreactivity in the postischemic rat brain cortex.
The hemodilution effect was evaluated in the parietal, temporal, and basal regions of postischemic rat brain neocortex by the immunohistochemical detection of the c-Fos protein. Rats were subjected to the four-vessel-occlusion and the hemodilution was used in one group of animals. Coronal sections of rat brains were used for immunohistochemical processing of c-Fos protein after different postischemic reperfusion intervals. The number of c-Fos positive neuronal nuclei was significantly decreased in the hemodilution group after 4 h of reperfusion in the parietal and basal neocortex compared to the reperfusion without hemodilution.
Miroslava
Némethová
N
Institute of Neurobiology, Slovak Academy of Sciences, Koice, Slovak Republic. nemeth@saske.sk
Burda
Jozef
J
Danielisová
Viera
V
Marala
Jozef
J
D000818:Animals; D000906:Antibodies; D017209:Apoptosis; D015415:Biomarkers; D001810:Blood Volume; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D006438:Hemodilution; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy
D000906:Antibodies; D015415:Biomarkers; D016760:Proto-Oncogene Proteins c-fos