Inhibitors of nitric oxide production from the flowers of Angelica furcijuga: structures of hyuganosides IV and V.
The methanolic extract from the flowers of Angelica furcijuga KITAGAWA was found to inhibit nitric oxide production in lipopolysaccharide-activated mouse peritoneal macrophages. From the methanolic extract, two new glycosides, hyuganosides IV and V, were isolated together with 28 known constituents. The structures of the new constituents were determined on the basis of chemical and physicochemical evidence. Furthermore, the inhibitory effects of 11 coumarin constituents on nitric oxide production were examined. Among them, 3'-angeloyl-cis-khellactone (IC(50)=82 microM), (S)-(-)-oxypeucedanin (57 microM), imperatorin (60 microM), isoepoxypteryxin (53 microM), and isopteryxin (8.8 microM) showed inhibitory activity.
Matsuda
Hisashi
H
Kyoto Pharmaceutical University, Kyoto.
Morikawa
Toshio
T
Ohgushi
Teruki
T
Ishiwada
Tomoko
T
Nishida
Norihisa
N
Yoshikawa
Masayuki
M
D029969:Angelica / Q000737:chemistry*; D000818:Animals; D001572:Benzofurans / Q000737:chemistry* / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000737:chemistry; D006868:Hydrolysis; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D010936:Plant Extracts / Q000737:chemistry; D013055:Spectrophotometry, Infrared
D001572:Benzofurans; D008070:Lipopolysaccharides; D010936:Plant Extracts; C502313:hyuganoside IV; C502174:hyuganoside V; D009569:Nitric Oxide