Monoamine oxidase inhibitory components from the roots of Sophora flavescens.
In our search for monoamine oxidase (MAO) inhibitors from natural resources, we found that the methanol extract of the roots of Sophora flavescens showed an inhibitory effect on mouse brain monoamine oxidase (MAO). Bioactivity-guided isolation of the extract yielded two known flavonoids, formononetin (1) and kushenol F (2), as active compounds along with three inactive compounds, oxymatrine (3), trifolirhizin (4), and beta-sitosterol (5). Formononetin (1) and kushenol F (2) showed significant inhibitory effects on MAO in a dose-dependent manner with IC50 values of 13.2 and 69.9 microM, respectively. Formononetin (1) showed a slightly more potent inhibitory effect against MAO-B (IC50: 11.0 microM) than MAO-A (IC50: 21.2 microM). Kushenol F (2) also preferentially inhibited the MAO-B activity than MAO-A activity with the IC50 values of 63.1 and 103.7 microM, respectively.
Hwang
Ji-Sang
JS
College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
Lee
Seon A
SA
Hong
Seong Su
SS
Lee
Kyong Soon
KS
Lee
Myung Koo
MK
Hwang
Bang Yeon
BY
Ro
Jai Seup
JS
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D008969:Molecular Sequence Data; D008996:Monoamine Oxidase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D007527:Isoenzymes; D008996:Monoamine Oxidase Inhibitors