[Phorbol 12-myristate 13-acetate inhibits apoptosis in erythroleukemia K562 cells induced by some nucleosides].
We studied the ability of phorbol 12-myristate 13-acetat to prevent erythroid differentiation and apoptosis in erythroleukemic K562 cells induced by cytidine, thymidine, and guanosine. The exposure of cancer cells to combinations of phorbol 12-myrsitate 13-acetate (100 nM) nucleosides for two days led to a loss of hemoglobin production (marker of erythroid differentiation) in cells and increased expression of monocyte-macrophage lineage associated surface antigen CD14. The treatment of K562 cells with nucleosides only was accompanied by the activation of caspase-3 and caspase-9, rather than caspase-6, increased fluorescence of ethidium bromide and DAPI upon binding to DNA, and apoptosis. Intracellular activation of caspase-6, inhibition of caspase-9, a markedly decreased activity of caspase-3 and of fluorescence of DNA-binding dyes, and inhibition of apoptosis were observed when the cells were treated with phorbol 12-myeristet 13-acetate combined with nucleosides.
Volkova
T O
TO
Malysheva
I E
IE
Nemova
N N
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D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D003562:Cytidine / Q000494:pharmacology; D003864:Depression, Chemical; D004789:Enzyme Activation; D041905:Erythroid Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006151:Guanosine / Q000494:pharmacology; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D009705:Nucleosides / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013936:Thymidine / Q000494:pharmacology
D006454:Hemoglobins; D018950:Lipopolysaccharide Receptors; D009705:Nucleosides; D006151:Guanosine; C016658:phorbolol myristate acetate; D003562:Cytidine; D020169:Caspases; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine