Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids.
An investigation of the liquid chromatography of the minor capsaicinoids in a commercial capsaicinoid mixture is reported. Twelve stationary phases including C8, C18, C30, phenyl, and cation-exchange chemistries were examined in combination with isocratic aqueous methanol and aqueous acetonitrile mobile phases. A phenyl stationary phase and aqueous acetonitrile mobile phase baseline-resolved 7 of 11 capsaicinoids, and selected ion chromatograms (LC-ESI-MS) demonstrated this was the most effective reversed-phase separation. Argentation chromatography with an alkyl or phenyl column and aqueous silver nitrate-methanol mobile phase revealed the presence of the 6-ene-8-methyl and 6-ene-9-methyl homocapsaicin isomers and the absence of 7-ene-9-methyl homocapsaicin. A mixed phenyl-cation-exchange stationary phase (charged with silver ion) enabled unique and useful separations of the capsaicinoids.
Thompson
Robert Q
RQ
Department of Chemistry, Oberlin College, 119 Woodland Street, Oberlin, OH 44074, USA.
Phinney
Karen W
KW
Sander
Lane C
LC
Welch
Michael J
MJ
D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D015394:Molecular Structure; D012834:Silver
D012834:Silver; D002211:Capsaicin