[A tetracycline-inducible gene system allows for regulated production of proteins with therapeutic potential].
The adoption of a bacterial system of control of genic expression with tetracycline, combined with the advances in the identification of regulatory sequences and mechanisms of expression of eukaryotic genes, has increased the versatility and effectiveness of techniques to reintroduce and to make genes function in cells and in superior organisms by being able to control the activity of the transgene in a temporary or reversible manner. This approach has also facilitated making detailed studies of different cellular processes and diseases; for example, the study of the function of oncogenes and other genes involved in the formation and progression of cancer, the generation of cellular models for recombinant protein production with therapeutic purposes, the ex-vivo genetic manipulation of extracted cells from patients and returned to them for gene therapy procedures, as well as the modulation of transgenes to revert hereditary suffering in animal models.
Castillo-Ureta
Hipólito
H
Laboratorio de Biología Celular, Departamento de Bioquímica de la Facultad de Medicina de la Universidad Autónoma de Nuevo León.
Barrera-Saldaña
Hugo A
HA
Martínez-Rodríguez
Herminia G
HG
D000818:Animals; D006801:Humans; D030781:Organisms, Genetically Modified; D014176:Protein Biosynthesis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013752:Tetracycline / Q000493:pharmacokinetics*; D019076:Transgenes / Q000187:drug effects*
D011994:Recombinant Proteins; D013752:Tetracycline