title: [Cloning and expression of superoxide dismutase gene from Deinococcus radiodurans in E. coli].
abstract: OBJECTIVE
  To construct expressing recombinant of Mn-SOD of Deinococcus radiodurans and express the target protein in E. coli BL21(DE3).
  
  
  METHODS
  SOD gene was amplified by PCR from genomic DNA of Deinococcus radiodurans and inserted into expression plasmid pET-30a(+) to create the recombinant pET-SOD. After being analyzed by the restriction endonuclease, the plasmid was transformed into E. coli BL21(DE3), and the recombinant protein was expressed after induction by the isopropyl-beta-D-thiogalactopyranoside (IPTG) and was analyzed with SDS-PAGE.
  
  
  RESULTS
  The recombinant plasmid pET-SOD was obtained, and the recombinant protein was highly expressed in E. coli BL21(DE3). The activity of recombinant superoxide dismutase was 51,800 U per gram of wet bacteria.
  
  
  CONCLUSIONS
  This study has provided a foundation for further studies and applications of the recombinant Mn-SOD.
authors.affiliation: Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Meng
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Dong-li
authors.initials: DL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhan
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Pei
mesh_terms: D003001:Cloning, Molecular; D034301:Deinococcus / Q000201:enzymology / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D007544:Isopropyl Thiogalactoside; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D007544:Isopropyl Thiogalactoside; D013482:Superoxide Dismutase