Identification of salt stress-induced transcripts in potato leaves by cDNA-AFLP.
Potato (Solanum tuberosum L.) is highly sensitive to salt stress, which is one of the most important factors limiting plant cultivation. The investigation of plant response to high salinity was envisaged in this report using cDNA-amplified fragment length polymorphism (AFLP). This technique was applied to salt- stressed and control potato plants (cv. Nicola). The expression profiles showed approx 5000 bands. Of these, 154 were upregulated and 120 were repressed by salt stress. In this study we have only considered cDNA fragments that seem to be originated from salt-induced mRNA. Eighteen fragments were then reamplified, cloned, and sequenced. Sequence comparison of these cDNA, identified in response to salt stress in potato, revealed that some of them present homologies with proteins in other species that are involved in cell wall structure and turnover such as proline-rich proteins and beta-galactosidase. A number of identified clones encoded putative stress response proteins such as NADP-dependant glyceraldehyde dehydrogenase and wound-induced protein. In addition, some of them encode proteins related to hypersensitive response against pathogens such as putative late blight and nematode as well as putative pathogenesis-related proteins. These cDNA seem to be differentially expressed in the presence of salt stress as shown by Northern blot or reverse Northern hybridization experiments.
Hmida-Sayari
Aïda
A
Laboratoire des Biotechnologies Végétales appliquées à l'amélioration des cultures, Département de Génie Biologique, Ecole Nationale d'Ingénieur de Sfax, B.P. W, 3038, Sfax, Tunisia.
Costa
Antonello
A
Leone
Antonella
A
Jaoua
Samir
S
Gargouri-Bouzid
Radhia
R
D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012965:Sodium Chloride / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D018076:DNA, Complementary; D012965:Sodium Chloride