title: Establishment of Schistosoma japonicum calpain-specific mouse T cell hybridomas and identification of a T cell epitope that stimulates IFNgamma production.
abstract: Calpain is a calcium-dependent cystein protease, and the homologues of schistosome are known as one of vaccine candidate molecules against schistosomiasis. Here, we established two IL-2 producing T cell hybridoma cell lines specific for Schistosoma japonicum calpain, to identify T cell epitope(s) on the molecule. Overlapping 15mer oligopeptides of calpain were synthesized and tested for their stimulatory abilities to the hybridomas. As a result, epitopes recognized by the two hybridoma lines were the same: EQLKIYAQRC. Spleen cells from calpain multiple antigenic peptide (MAP)-immunized BALB/c mice produced IFNgamma upon stimulation with MAP or soluble worm antigen preparation (SWAP). The identification of the T cell epitope to stimulate Th1 response will contribute to the proper design of synthetic vaccines, evaluation of their protective potentials and elucidation of protective mechanisms in murine experimental schistosomiasis.
authors.affiliation: Department of Parasitology and Tropical Public Health, The University of Occupational and Environmental Health, Japan, Kitakyushu 807-8555, Japan. yosada@med.uoeh-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Osada
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumagai
authors.forename: Mariko
authors.initials: M
authors.lastname: Hato
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Malky
authors.forename: Hiroko
authors.initials: H
authors.lastname: Asahi
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ohta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002154:Calpain / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte; D005260:Female; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D012549:Schistosoma japonicum / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D007371:Interferon-gamma; D002154:Calpain