High-resolution magic-angle spinning 13C NMR spectroscopy of cerebral tissue.
Monitoring the metabolism of (13)C-labelled substrates by biological tissues allows both the rate of metabolism and the relative importance of metabolic pathways to be determined. In this study high-resolution magic-angle spinning (HRMAS) (13)C NMR spectroscopy is assessed as a technique for determining the labelling of metabolites in brain slices. Freshly prepared rat brain slices were superfused in isotonic salt solution containing [1-(13)C] glucose. HRMAS (1)H and (13)C NMR spectra were acquired of the slices ( approximately 10 mg) at 3 degrees C. Using (1)H NMR spectroscopy it was demonstrated that the concentration of key metabolites indicative of metabolic degradation, including N-acetyl aspartate and lactate, did not change significantly across the approximately 11 h time period required for (13)C NMR spectra. The approach produced high-resolution spectra of intact tissue with the labelling patterns of tissues being indicative of both labelling via pyruvate dehydrogenase found in both neuronal and glial cells, and pyruvate carboxylase, found only within glial cells. This approach is a versatile tool for monitoring the compartmentation of metabolites directly, and will also allow the investigation of aqueous and lipid metabolites simultaneously.
Griffin
J L
JL
Department of Biochemistry, University of Cambridge, UK. jlg40@mole.bio.cam.ac.uk
Corcoran
O
O
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D005260:Female; D005947:Glucose / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D012815:Signal Processing, Computer-Assisted; D013113:Spin Labels
D002247:Carbon Isotopes; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D013113:Spin Labels; D019344:Lactic Acid; D018698:Glutamic Acid; D005947:Glucose