Optimization of a new preservation solution for machine perfusion of the liver: which is the preferred colloid?
OBJECTIVE
Machine perfusion (MP) has proven to be beneficial in experimental preservation of the liver. The modified University of Wisconsin solution (UW-Gluconate or UW-G) is used as the MP preservation solution of choice. We have developed Polysol, an enriched MP preservation solution based on a colloid. We sought to optimize Polysol by substituting the colloid hydroxyethylstarch (HES) with the colloids dextran and polyethylene glycol (PEG).


METHODS
In an isolated perfused rat liver model, hepatocellular damage and liver function were assessed during reperfusion with Krebs-Henseleit buffer after 24 hours hypothermic MP using Polysol-HES, Polysol-dextran, or Polysol-PEG. Control livers were preserved by MP using UW-G.


RESULTS
Compared to MP-UW-G, MP using Polysol resulted in significantly less damage and improved function during reperfusion. MP using Polysol-dextran or Polysol-PEG resulted in equal or less damage than Polysol-HES. Differences in ammonia clearance and bile production were not significant. Tissue edema was higher after MP using Polysol-HES as compared to Polysol-dextran and Polysol-PEG.


CONCLUSIONS
MP of rat livers for 24 hours using UW-G results in more extensive damage and reduced liver function compared to MP using Polysol. MP using Polysol-dextran or Polysol-PEG results in equal or even better preservation compared to Polysol-HES.
Bessems
M
M
Surgical Laboratory, Academic Medical Center, Amsterdam, Netherlands. m.bessems@amc.uva.nl
Doorschodt
B M
BM
Hooijschuur
O
O
van Vliet
A K
AK
van Gulik
T M
TM
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003102:Colloids; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000473:pathology / Q000502:physiology; D008111:Liver Function Tests; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods*; D011887:Raffinose; D051381:Rats
D003102:Colloids; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose