title: Role of curvature and phase transition in lipid sorting and fission of membrane tubules.
abstract: We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed in vivo with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is observed when phase separation is generated in the tubes. This shows that lipid sorting may depend critically on both membrane curvature and phase separation. Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes.
authors.affiliation: UMR 144 CNRS/Institut Curie, Paris, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Roux
authors.forename: Damien
authors.initials: D
authors.lastname: Cuvelier
authors.forename: Pierre
authors.initials: P
authors.lastname: Nassoy
authors.forename: Jacques
authors.initials: J
authors.lastname: Prost
authors.forename: Patricia
authors.initials: P
authors.lastname: Bassereau
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
mesh_terms: D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry* / Q000378:metabolism; D004548:Elasticity; D006056:Golgi Apparatus / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism; D010777:Photochemistry; D013109:Sphingomyelins / Q000737:chemistry* / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D002784:Cholesterol