Gender differences in antioxidant capacity of rat tissues determined by 2,2'-azinobis (3-ethylbenzothiazoline 6-sulfonate; ABTS) and ferric reducing antioxidant power (FRAP) assays.
Differences in susceptibility to oxidative stress between males and females have been postulated. Several methods have been developed to assess the total antioxidant capacity of human serum or plasma, but just recently some of them were employed for measurement of antioxidant capacity of tissues. In this study, we measured and compared antioxidant capacity of heart, kidney, liver and brain tissues of male and female rats. Antioxidant capacity was determined using 2,2'-azinobis (3-ethylbenzothiazoline 6-sulfonate; ABTS) and ferric reducing antioxidant power (FRAP) assays. In the same samples, lipid peroxidation products of these tissues were analysed using thiobarbituric acid reactive substances (TBARS) assays. Antioxidant capacity of heart, kidney and liver tissues was higher in female than male rats for both FRAP and ABTS assays. We found positive correlation between FRAP and ABTS values for all tested tissues. FRAP and ABTS proved to be comparable, simple and quick methods for antioxidant capacity scanning in tissues. TBARS levels differed only for brain tissue, being higher in males. These results indicate stronger defense against oxidative damage in females for all observed tissues. These finding may account for the longer lifespan of females.
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Faculty of Chemical Technology, Teslina 10, Split, Croatia.
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D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D052160:Benzothiazoles; D002712:Chlorides; D005260:Female; D005290:Ferric Compounds / Q000032:analysis*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013451:Sulfonic Acids / Q000032:analysis*; D014018:Tissue Distribution / Q000502:physiology
D000975:Antioxidants; D052160:Benzothiazoles; D002712:Chlorides; D005290:Ferric Compounds; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C024555:ferric chloride