Strategies for the reconstitution of chromatin.
In eukaryotes, chromatin is the natural form of DNA in the nucleus. For hundreds of millions of years, DNA-binding factors have evolved with chromatin. It is therefore more desirable to study the molecular mechanisms of DNA-directed processes with chromatin than with naked DNA templates. To this end, it is necessary to reconstitute DNA and histones into chromatin. Fortunately, there are a variety of methods by which a nonspecialist can prepare chromatin of high quality. Here, we describe strategies and techniques for the reconstitution of chromatin in vitro.
Lusser
Alexandra
A
Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0347, USA.
Kadonaga
James T
JT
D000818:Animals; D032701:Biomimetics / Q000379:methods*; D002843:Chromatin / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D042002:Chromatin Assembly and Disassembly; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D006801:Humans; D009707:Nucleosomes / Q000737:chemistry*
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes