Gene expression profiling reveals sequential changes in gastric tubular adenoma and carcinoma in situ.
OBJECTIVE
To analyze the expression profiles of premalignant and/or preclinical lesions of gastric cancers.


METHODS
We analyzed the expression profiles of normal gastric pit, tubular adenoma and carcinoma in situ using microdissected cells from routine gastric biopsies. For the DNA microarray analysis of formalin-fixed samples, we developed a simple and reproducible RNA extraction and linear amplification procedure applying two polymerase-binding sites. The amplification procedure took only 8 h and yielded comparable DNA microarray data between formalin-fixed tissues and unfixed controls.


RESULTS
In comparison with normal pit, adenoma/carcinoma showed 504 up-regulated and 29 down-regulated genes at the expected false significance rate 0.15%. The differential expression between adenoma and carcinoma in situ was subtle: 50 and 22 genes were up-, and down-regulated in carcinomas at the expected false significance rate of 0.61%, respectively. Differentially expressed genes were grouped according to patterns of the sequential changes for the 'tendency analysis' in the gastric mucosa-adenoma-carcinoma sequence.


CONCLUSIONS
Groups of genes are shown to reflect the sequential expression changes in the early carcinogenic steps of stomach cancer. It is suggested that molecular carcinogenic pathways could be analyzed using routinely processed biopsies.
Lee
Chang-Hee
CH
Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 138-736, Korea.
Bang
Seung-Hyun
SH
Lee
Seung-Koo
SK
Song
Kyu-Young
KY
Lee
In-Chul
IC
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D000818:Animals; D002278:Carcinoma in Situ / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014183:Transplantation, Heterologous