Organic solvent high-field amplified stacking for basic compounds in capillary electrophoresis.
Many water-miscible organic solvents, especially acetonitrile and acetone, bring along significant degrees (approximately 30 times) of stacking by electroinjection through high-field amplified injection for the basic compounds compared to that for aqueous buffers or water. The relative stacking of different compounds in acetonitrile or acetone is different compared to that for water. Stacking by electroinjection in organic solvents is less stringent and easier to accomplish in practice. Acids and salts, in aqueous solutions, can ruin the stacking for both organic and aqueous solvents; however, this effect can be better tolerated by diluting the sample in acetonitrile. Thus, this stacking is termed "organic solvent high-field amplified injection". This stacking by electroinjection is enhanced by increasing the electrophoresis buffer concentration and can be better than that by pressure injection. From the practical aspects, some cationic drugs present in serum such as amiodarone can be detected at the therapeutic levels by electroinjection on the capillary after protein precipitation by acetonitrile.
Shihabi
Zak K
ZK
Department of Pathology, Wake Forest University School of Medicine, Baptist Medical Center, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
D000096:Acetone / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002021:Buffers; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002627:Chemistry, Physical; D019075:Electrophoresis, Capillary / Q000379:methods*; D011506:Proteins; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D002021:Buffers; D011506:Proteins; D012997:Solvents; D014867:Water; D000096:Acetone; C032159:acetonitrile