title: Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state.
abstract: The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
authors.affiliation: Institute of Pharmaceutical Chemistry, University of Milano, Italy. roberto.artali@tiscali.it
authors.forename: R
authors.initials: R
authors.lastname: Artali
authors.forename: N
authors.initials: N
authors.lastname: Marchini
authors.forename: F
authors.initials: F
authors.lastname: Meneghetti
authors.forename: D
authors.initials: D
authors.lastname: Cavazzini
authors.forename: A
authors.initials: A
authors.lastname: Cassetta
authors.forename: C
authors.initials: C
authors.lastname: Sassone
mesh_terms: D019943:Amino Acid Substitution; D016227:Benzoquinones / Q000737:chemistry; D018360:Crystallography, X-Ray; D045285:Desulfovibrio desulfuricans / Q000737:chemistry / Q000235:genetics*; D005418:Flavodoxin / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D013056:Spectrophotometry, Ultraviolet
chemical_list: D016227:Benzoquinones; D005418:Flavodoxin; C025232:semiquinone radicals