Altered T helper 1 reaction but not increase of virus load in patients with dengue hemorrhagic fever.
To investigate whether dengue-2 patients with and without dengue hemorrhagic fever had different virus load, immune mediators, or T helper (Th) reaction, we simultaneously measured virus load, immune mediators and the Th1/Th2 transcription factors T-bet/GATA-3 mRNA expression in a large outbreak of dengue-2 infections in Southern Taiwan. Results showed that virus load was not significantly different between patients with and without dengue hemorrhagic fever. Patients with dengue fever had higher IFN-gamma levels, but patients with dengue hemorrhagic fever had significantly higher IL-10 levels. Further studies showed that patients with dengue hemorrhagic fever had a significantly lower T-bet than those with dengue fever, but GATA-3 mRNA expression in peripheral blood leukocytes was not significant difference between both groups. In conclusion, altered Th1 reaction as reflected by lower T-bet mRNA expression associated with higher IL-10 levels might be involved in the pathogenesis of dengue hemorrhagic fever.
Chen
Rong-Fu
RF
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
Liu
Jien-Wei
JW
Yeh
Wen-Ting
WT
Wang
Lin
L
Chang
Jen-Chieh
JC
Yu
Hong-Ren
HR
Cheng
Jiin-Tsuey
JT
Yang
Kuender D
KD
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood; D004268:DNA-Binding Proteins / Q000235:genetics; D003715:Dengue / Q000235:genetics / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000302:isolation & purification; D005260:Female; D050990:GATA3 Transcription Factor; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D019595:Severe Dengue / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D020825:T-Box Domain Proteins; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014766:Viremia / Q000235:genetics / Q000276:immunology / Q000821:virology; D000098815:T-bet Transcription Factor
D016207:Cytokines; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; D016753:Interleukin-10