Cold exposure differently influences mitochondrial energy efficiency in rat liver and skeletal muscle.
This study deals with mitochondrial energy efficiency in liver and skeletal muscle mitochondria in 15 days cold exposed rats. Cold exposure strongly increases the sensitivity to uncoupling by added palmitate of skeletal muscle but not liver mitochondria, while mitochondrial energy coupling in the absence of fatty acids is only slightly affected by cold in liver and skeletal muscle. In addition, uncoupling protein 3 content does not follow changes in skeletal muscle mitochondrial coupling. It is therefore concluded that skeletal muscle could play a direct thermogenic role based on fatty acid-induced mild uncoupling of mitochondrial oxidative phosphorylation.
Mollica
Maria Pina
MP
Department of Biological Sciences, Section of Physiology, University of Naples "Federico II", Via Mezzocannone 8, I-80134 Naples, Italy.
Lionetti
Lillà
L
Crescenzo
Raffaella
R
Tasso
Rosaria
R
Barletta
Antonio
A
Liverini
Giovanna
G
Iossa
Susanna
S
D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism; D003080:Cold Temperature; D003576:Electron Transport Complex IV / Q000032:analysis / Q000378:metabolism; D007473:Ion Channels; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000032:analysis / Q000378:metabolism; D024101:Mitochondrial Proteins; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption; D010168:Palmitates / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014475:Uncoupling Agents / Q000494:pharmacology; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D010168:Palmitates; C000604394:Ucp3 protein, rat; D014475:Uncoupling Agents; D000071247:Uncoupling Protein 3; D000226:Mitochondrial ADP, ATP Translocases; D003576:Electron Transport Complex IV