[Blood circulation and secretory function in the gastric mucosa during inhibition of M1-cholinergic receptors and L-Ca2+-channels].
The gastric secretion and gastric mucosal blood flow (GMBF) under simultaneous action of M1-cholinergic antagonist gastrocepine (3 mg/kg i.v.) and L-Ca2+-channels blocker verapamile (1.25 mg/kg i.v.) were investigated in urethane-anaesthetized rats. The stomach was perfused with saline, and GMBF was measured by using hydrogen gas clearance technique. The acid production under combined action of both blockers was lower by 57.3% and 18.6%, than in case of separate action of gastrocepine and verapamile accordingly. Pepsin concentration decreased by 32%, which did not differ from verapamile action alone. The above changes of gastric secretion was accompanied by significant decrease of GMBF. Inhibition of M1-cholinergic receptors by gastrocepine did not show their key role in basal gastric secretion in rats, but they exacerbate L-Ca2+-channels blocker action. We conclude, that enhance of acid output inhibition is related to the simultaneous depression of nervous impulses in ganglionic neurons and acethylcholine release from parasympathetic postganglionic neurons. Synchronous block of M1-cholinergic receptors and L-Ca2+-channels of smooth muscles stomach vessels resulted in reducing of gastric mucosal blood flow.
Skliarov
O Ia
OIa
Mandryk
Iu V
IuV
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D004357:Drug Synergism; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D010890:Pirenzepine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D043643:Receptor, Muscarinic M1 / Q000037:antagonists & inhibitors*; D013152:Splanchnic Circulation / Q000187:drug effects / Q000502:physiology*; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D018727:Muscarinic Antagonists; D043643:Receptor, Muscarinic M1; D010890:Pirenzepine; D014700:Verapamil