Specific detection of the PVY(N)-W variant of Potato virus Y.
PVY(N)-W is one of the variant populations of Potato virus Y (PVY). This variant is of concern in seed potato production and requires a specific diagnosis since it induces more or less symptomless infections and is not detectable easily in field inspections. Moreover, this variant is serologically indistinguishable from the common strain PVY(O). This study describes a simple and specific molecular detection test for the PVY(N)-W variant using a PCR protocol based on the recombinant point within the HC-Pro/P3 region of PVY(N) variants (PVY(NTN), PVY(N)-W). To avoid both detection of recombinant PVY(NTN) and PVY(N)-W isolates, a forward PVY(N)-like primer located in the HC-Pro region coupled to a reverse PVY(O)-like primer located in the NIa region was designed to amplify a specific PCR product of 4114 nt from PVY(N)-W isolates. This technique was assessed on 41 PVY reference and field isolates. Only isolates referenced as PVY(N)-W were amplified and gave the expected PCR product of 4114 nt, whereas no band was obtained from PVY(N), PVY(NTN) or PVY(O) isolates. In conclusion, this PVY(N)-W diagnosis tool is rapid, easy-to-use and suitable for large-scale testing in laboratories of seed potato certification.
Glais
L
L
Institut National de la Recherche Agronomique, centre de Rennes, INRA/AGROCAMPUS UMR BiO3P, F-35653 Le Rheu, France.
Tribodet
M
M
Kerlan
C
C
D017931:DNA Primers; D014644:Genetic Variation; D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017800:Potyvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D011198:Solanum tuberosum / Q000821:virology*
D017931:DNA Primers