A fluorescent inositol phosphate glycan stimulates lipogenesis in rat adipocytes by extracellular activation alone.
The chemical synthesis of 2,6-dideoxy-2-amino-6-mercaptoglucopyranosyl-(alpha1-6)-myo-inositol 1,2-cyclic phosphate and its conjugation with a lucifer yellow derivative are reported. The resulting fluorescent IPG analogue was able to stimulate lipogenesis in rat adipocytes despite the fact that it was not internalized into the cell. The results demonstrate that internalization of the IPG is not required for manifestation of its insulin-like effects.
Turner
David I
DI
Michael Chemistry Laboratory, Department of Chemistry, Tufts University, Medford, MA 02155, USA.
Chakraborty
Nilanjana
N
d'Alarcao
Marc
M
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D045604:Extracellular Fluid / Q000187:drug effects* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D008055:Lipids; D011134:Polysaccharides; C028039:inositol phosphate glycan