19F NMR measurements of NO production in hypertensive ISIAH and OXYS rats.
Recently we demonstrated the principal possibility of application of 19F NMR spin-trapping technique for in vivo *NO detection [Free Radic. Biol. Med. 36 (2004) 248]. In the present study, we employed this method to elucidate the significance of *NO availability in animal models of hypertension. In vivo *NO-induced conversion of the hydroxylamine of the fluorinated nitronyl nitroxide (HNN) to the hydroxylamine of the iminonitroxide (HIN) in hypertensive ISIAH and OXYS rat strains and normotensive Wistar rat strain was measured. Significantly lower HIN/HNN ratios were measured in the blood of the hypertensive rats. The NMR data were found to positively correlate with the levels of nitrite/nitrate evaluated by Griess method and negatively correlate with the blood pressure. In comparison with other traditionally used methods 19F NMR spectroscopy allows in vivo evaluation of *NO production and provides the basis for in vivo *NO imaging.
Bobko
Andrey A
AA
Institute of Chemical Kinetics and Combustion, Novosibirsk 630090, Russia. bobko@kinetics.nsc.ru
Sergeeva
Svetlana V
SV
Bagryanskaya
Elena G
EG
Markel
Arkadii L
AL
Khramtsov
Valery V
VV
Reznikov
Vladimir A
VA
Kolosova
Nataljya G
NG
D000818:Animals; D005461:Fluorine; D006973:Hypertension / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats
D005461:Fluorine; D009569:Nitric Oxide