[Analysis of residual solvents in natural flavorings by headspace GC using the standard addition method].
Headspace GC using the standard addition method has been developed for the simultaneous determination of organic solvents in natural flavorings. The procedure can be outlined as follows: an aliquot of the sample is transferred to a 10 mL vial. To each vial, a DMSO solution containing solvents at different concentrations is added as the standard solution. The vials are kept at 50 degrees C (for automatic injection) or 40 degrees C (for hand-operated injection) for 40 minutes. One mL of the vapor phase in each vial is injected into a gas chromatograph equipped with an Aquatic-2 column (0.25 mm i.d. x 60 m). To evaluate this method, we conducted a performance study in collaboration with 10 laboratories, using ginger oleoresin. We analyzed 6 solvents (methanol, 2-propanol, acetone, dichloromethane, hexane, and 1,1,2-trichloroethene) for which the maximum residue limits are established in Japan's Specifications and Standards for Food Additives. Methanol and acetone existed in the ginger oleoresin, so only the other that four kinds of solvents were added to it. Eight of the laboratories used automatic injection, while the remaining two used hand-operated injection. Statistical analyses were conducted on the data obtained from the 8 laboratories. Repeatability standard deviations (RSDr) ranged from 4.3 to 11.4%, and reproducibility standard deviations (RSDR) ranged from 8.4 to 19.0%.
Sato
Kyoko
K
National Institute of Health Sciences, Tokyo, Japan.
Uematsu
Yoko
Y
Isagawa
Satoshi
S
Tateba
Hideki
H
Tomizawa
Masahito
M
Oosaki
Kazuhiko
K
Hasebe
Akio
A
Shibuya
Saburo
S
Nii
Hiromichi
H
Higashinaka
Ryuji
R
Watanabe
Ichiro
I
Yamazaki
Takeshi
T
Tanamoto
Ken-ichi
K
Maitani
Tamio
T
D019840:2-Propanol / Q000032:analysis; D000096:Acetone / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D005421:Flavoring Agents / Q000737:chemistry*; D020939:Zingiber officinale; D006586:Hexanes / Q000032:analysis; D000432:Methanol / Q000032:analysis; D008752:Methylene Chloride / Q000032:analysis; D012997:Solvents / Q000032:analysis*; D014240:Trichloroethanes / Q000032:analysis
D005421:Flavoring Agents; D006586:Hexanes; D012997:Solvents; D014240:Trichloroethanes; D000096:Acetone; C024567:1,1,2-trichloroethane; D008752:Methylene Chloride; D019840:2-Propanol; D000432:Methanol