Comparison of hyaluronate/carboxymethylcellulose membrane and melatonin for prevention of adhesion formation in a rat model.
BACKGROUND
To investigate the effectiveness of melatonin in preventing post-operative adhesion formation and to compare it with the efficacy of hyaluronate/carboxymethylcellulose membrane in a rat model.


METHODS
Following pilot studies, 35 rats were operated on in the full study. In 15 animals (group one), 10 standard lesions were inflicted in each uterine horn (total 30 horns) and melatonin was applied before closure of the abdomen. In the second group, 20 animals were operated on and one of the uterine horns (total 20 horns) with standard lesions was treated with hyaluronate/carboxymethylcellulose membrane and the other uterine horn served as a control. Second-look operations were performed 1 week later and adhesion scores were compared.


RESULTS
The adhesion scores of uterine horns treated with melatonin and of uterine horns treated with hyaluronate/carboxymethylcellulose membrane were significantly lower than the scores of the controls (P < 0.001). There was no statistically significant difference between the adhesion scores of uterine horns treated with melatonin and of uterine horns treated with hyaluronate/carboxymethylcellulose membrane (P > 0.05).


CONCLUSIONS
Both melatonin and hyaluronate/carboxymethylcellulose membrane were effective in prevention of adhesion formation in a rat uterine horn model.
Demirbag
Suzi
S
Department of Paediatric Surgery and Research Centre, Gülhane Military Medical Academy, Ankara, Turkey. sdemirbag@gata.edu.tr
Cetinkursun
Salih
S
Tasdemir
Umut
U
Ozturk
Haluk
H
Pekcan
Mesut
M
Yesildaglar
Narter
N
D000818:Animals; D002266:Carboxymethylcellulose Sodium; D004195:Disease Models, Animal; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage; D006820:Hyaluronic Acid; D008550:Melatonin / Q000008:administration & dosage*; D008567:Membranes, Artificial; D010532:Peritoneal Diseases / Q000517:prevention & control; D011183:Postoperative Complications / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control*
D016166:Free Radical Scavengers; D008567:Membranes, Artificial; D006820:Hyaluronic Acid; D008550:Melatonin; D002266:Carboxymethylcellulose Sodium