Insulin-like growth factors I and II in the aseptic loosening of total hip implants.
Periprosthetic interface tissue and pseudocapsule samples surrounding aseptically loosened hip implants and control knee synovium were studied by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Endothelial cells, fibroblasts, and monocyte/macrophages contained bone formation-enhancing insulin-like growth factors (IGFs). In interface tissue we found fewer IGF-I and IGF-II positive cells than in control tissue. In pseudocapsular tissue we found fewer IGF-I positive cells and an equal amount of IGF-II positive cells compared to control tissues. Decreased bone formation may contribute to net loss of bone around aseptically loosened hip implants.
Waris
V
V
Molecular Biology Laboratory, Musculoskeletal Diseases and Inflammation Research Group (TULES RYHMA), Institute of Biomedicine, Department of Anatomy, University of Helsinki, Helsinki, Finland. Ville.Waris@helsinki.fi
Zhao
D S
DS
Leminen
H
H
Santavirta
S
S
Takagi
M
M
Nordsletten
L
L
Konttinen
Y T
YT
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects*; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D016022:Case-Control Studies; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000378:metabolism*; D007593:Joint Instability / Q000175:diagnosis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011475:Prosthesis Failure; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018570:Risk Assessment; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology
D015415:Biomarkers; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II