[Effects of heating on the epithelia of benign prostate hyperplasia in vitro].
OBJECTIVE
To investigate the biological changes of the epithelia of benign prostate hyperplasia under heating at different temperatures and at different points of time after heating.


METHODS
Cell morphology, MTT, flow cytometer, and the immunocytochemical method for detecting heat shock protein 70 and prostate specific antigen were used to observe the effect of heating on the primarily cultured epithelia of benign prostate hyperplasia at 45 degrees C and 60 degrees C and at 1 hour and 12 hours after heating.


RESULTS
Heating at 45 degrees C for 15 min resulted in apoptosis, and at 60 degrees C, necrosis in most of the cells. The inhibiting effect of heating on the growth of cells was observed, more significant in the 60 degrees C group than in the 45 degrees C group. The cell phase arrest induced by heat, mainly G0/G1 arrest, was more significant in the 12 h group than in the 1 h group. Heating up-regulated the expressions of heat shock protein 70 and prostate specific antigen in cells.


CONCLUSIONS
Heating can induce apoptosis, necrosis and growth suppression of the epithelia of benign prostate hyperplasia. Its process and mechanism are correlated with the cell phase arrest and the up-regulation of the expressions of heat shock protein 70 and prostate specific antigen.
Sun
Ming
M
Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. sunming2003@163.com
Yang
Yu-ru
YR
Li
Hong
H
Lu
Yi-ping
YP
Wei
Qiang
Q
Shen
Hong
H
Shi
Ming
M
D017209:Apoptosis; D002453:Cell Cycle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D006979:Hyperthermia, Induced; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis; D011470:Prostatic Hyperplasia / Q000473:pathology*
D018840:HSP70 Heat-Shock Proteins; D017430:Prostate-Specific Antigen