Near-IR absorption and fluorescence spectra and AFM observation of the light-harvesting 1 complex on a mica substrate refolded from the subunit light-harvesting 1 complexes of photosynthetic bacteria Rhodospirillum rubrum.
The subunit light-harvesting 1 (LH 1) complexes isolated from photosynthetic bacteria Rhodospirillum rubrum using n-octyl-beta-glucoside were reassociated and adsorbed on a mica substrate using spin-coat methods with the aim of using this LH complex in a nanodevice. The near-IR absorption and fluorescence spectra of the LH 1 complexes indicated that the LH 1 complex on the mica was stable, and efficient energy transfer from a carotenoid to a bacteriochlorophyll a was observed. Atomic force microscopy of the reassociated LH 1 complexes, under air, showed the expected ringlike structure. The outer and inner diameters of the ringlike structure of the LH 1 complex were approximately 30 and 8 nm, respectively, and the ringlike structure protruded by 0.2-0.6 nm.
Iida
Kouji
K
Nagoya Municipal Industrial Research Institute, Atsuta-ku Rokuban, Nagoya 456-0058, Japan. iida.kouji@nmiri.city.nagoya.jp
Inagaki
Jun-Ichi
J
Shinohara
Kiyoshi
K
Suemori
Yoshiharu
Y
Ogawa
Makiko
M
Dewa
Takehisa
T
Nango
Mamoru
M
D000538:Aluminum Silicates; D000595:Amino Acid Sequence; D002338:Carotenoids; D017124:Conserved Sequence; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D008823:Micelles; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D011489:Protein Denaturation; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012247:Rhodospirillum rubrum / Q000201:enzymology* / Q000648:ultrastructure; D016415:Sequence Alignment; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013379:Substrate Specificity
D000538:Aluminum Silicates; D045342:Light-Harvesting Protein Complexes; D008823:Micelles; D045331:Photosystem I Protein Complex; D021122:Protein Subunits; D002338:Carotenoids; C011934:mica