Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography.
A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi (Huang-qin in Chinese) was successfully established by using ethyl acetate-methanol-1% acetic acid water (5:0.5:5, v/v) as the two-phase solvent system. The upper phase of ethyl acetate-methanol-1% acetic acid water (5:0.5:5, v/v) was used as the stationary phase of HSCCC. Baicalin (58.1 mg) and wogonoside (17.0mg) with the purity of 99.2 and 99.0%, respectively, were separated successfully in one-step separation from 120 mg of crude sample from S. baicalensi, Georgi. The structures of baicalin and wogonoside were identified by 1H NMR and 13C NMR.
Wu
Sujuan
S
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
Sun
Ailing
A
Liu
Renmin
R
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D044950:Flavanones / Q000302:isolation & purification*; D005419:Flavonoids / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012997:Solvents
D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D012997:Solvents; C038044:baicalin; C473995:wogonoside