Agrobacterium tumefaciens-mediated transformation of leek (Allium porrum) and garlic (Allium sativum).
Transgenic leek (Allium porrum) and garlic (Allium sativum) plants have been recovered by the selective culturing of immature leek and garlic embryos via Agrobacterium-mediated transformation using a method similar to that described by Eady et al. (Plant Cell Rep 19:376-381, 2000) for onion transformation. This method involved the use of a binary vector containing the m-gfp-ER reporter gene and nptII selectable marker, and followed the protocol developed previously for the transformation of onions with only minor modifications pertaining to the post-transformation selection procedure which was simplified to have just a single selection regime. Transgenic cultures were selected for their ability to express the m-gfp-ER reporter gene and grown in the presence of geneticin (20 mg/l). The presence of transgenes in the genome of the plants was confirmed using TAIL-PCR and Southern analysis. This is the first report of leek and "true seed" garlic transformation. It now makes possible the integration of useful agronomic and quality traits into these crops.
Eady
Colin
C
New Zealand Institute for Crop & Food Research Limited, Private bag Box 4704, Christchurch, New Zealand. eadyc@crop.cri.nz
Davis
Sheree
S
Catanach
Andrew
A
Kenel
Fernand
F
Hunger
Sarah
S
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D003049:Coccidiostats / Q000494:pharmacology; D005737:Garlic / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D018745:Genome, Plant; D005839:Gentamicins / Q000494:pharmacology; D019697:Onions / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic / Q000235:genetics*; D019076:Transgenes / Q000235:genetics
D003049:Coccidiostats; D005819:Genetic Markers; D005839:Gentamicins; C010680:antibiotic G 418