title: Gene expression profiling of cerebellar development with high-throughput functional analysis.
abstract: We measured the expression levels of 450 genes during mouse postnatal cerebellar development by quantitative PCR using RNA purified from layers of the cerebellar cortex. Principal component analysis of the data matrix demonstrated that the first and second components corresponded to general levels of gene expression and gene expression patterns, respectively. We introduced 288 of the 450 genes into PC12 cells using a high-throughput transfection assay based on atelocollagen and determined the ability of each gene to promote neurite outgrowth or cell proliferation. Five genes induced neurite outgrowth, and seven genes enhanced proliferation. Evaluation of the functional data and gene expression patterns showed that none of these genes exhibited elevated expression at maturation, suggesting that genes characteristic of mature neurons are not likely to participate in neuronal development. These results demonstrate that functional data can facilitate interpretation of expression profiles and identification of new molecules that participate in biological processes.
authors.affiliation: Taisho Laboratory of Functional Genomics, Nara Institute of Science and Technology, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo, Japan.
authors.forename: Sakae
authors.initials: S
authors.lastname: Saito
authors.forename: Kimi
authors.initials: K
authors.lastname: Honma
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kita-Matsuo
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.forename: Kikuya
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D016716:PC12 Cells; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger