S100A1 and KIT gene expressions in common subtypes of renal tumours.
OBJECTIVE
The aim of this study is to evaluate the S100A1 and KIT as gene markers for the differentiation of common subtypes of renal tumours.


METHODS
Fifty-five tissue samples (15 clear cell RCCs, 15 papillary RCCs, 7 chromophobe RCCs, 8 oncocytomas and 10 normal renal tissues) were studied The gene expressions of S100A1 and KIT were analysed by one-step RT-PCR by using the specific primers.


RESULTS
S100A1 was expressed in 2/15 clear cell RCCs, 11/15 papillary RCCs, 7/8 oncocytomas and in 0/7 chromophobe RCCs. KIT gene was expressed in 6/7 chromophobe RCCs and 7/8 oncocytomas while 0/15 clear cell RCCs and 1/15 papillary RCCs expressed kit gene. Normal tissue expressed neither S100A1 nor KIT gene.


CONCLUSIONS
S100A1 and KIT can be used as gene markers for the differentiation of common subtypes of renal tumours.
Li
G
G
Department of Urology, North Hospital, CHU of Saint-Etienne, 42055 Saint-Etienne Cedex 2, France. grli2001@yahoo.fr
Gentil-Perret
A
A
Lambert
C
C
Genin
C
C
Tostain
J
J
D018262:Adenocarcinoma, Clear Cell / Q000737:chemistry; D018249:Adenoma, Oxyphilic / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis*; D002291:Carcinoma, Papillary / Q000737:chemistry; D002292:Carcinoma, Renal Cell / Q000737:chemistry; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry*; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009418:S100 Proteins / Q000032:analysis* / Q000235:genetics
D014408:Biomarkers, Tumor; D009418:S100 Proteins; C072337:S100A11 protein, human; D019009:Proto-Oncogene Proteins c-kit