title: Diacylglycerol pyrophosphate is a second messenger of abscisic acid signaling in Arabidopsis thaliana suspension cells.
abstract: In plants, the importance of phospholipid signaling in responses to environmental stresses is becoming well documented. The involvement of phospholipids in abscisic acid (ABA) responses is also established. In a previous study, we demonstrated that the stimulation of phospholipase D (PLD) activity and plasma membrane anion currents by ABA were both required for RAB18 expression in Arabidopsis thaliana suspension cells. In this study, we show that the total lipids extracted from ABA-treated cells mimic ABA in activating plasmalemma anion currents and induction of RAB18 expression. Moreover, ABA evokes within 5 min a transient 1.7-fold increase in phosphatidic acid (PA) followed by a sevenfold increase in diacylglycerol pyrophosphate (DGPP) at 20 min. PA activated plasmalemma anion currents but was incapable of triggering RAB18 expression. By contrast, DGPP mimicked ABA on anion currents and was also able to stimulate RAB18 expression. Here we show the role of DGPP as phospholipid second messenger in ABA signaling.
authors.affiliation: Physiologie Cellulaire et Moléculaire des Plantes, Unité Mixte de Recherche 7632-Centre National de la Recherche Scientifique, case 156, Université Pierre et Marie Curie, Le Raphaël, 3 rue Galilée 94200 Ivry sur Seine, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Zalejski
authors.forename: Zongshen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Quettier
authors.forename: Régis
authors.initials: R
authors.lastname: Maldiney
authors.forename: Magda
authors.initials: M
authors.lastname: Bonnet
authors.forename: Mathias
authors.initials: M
authors.lastname: Brault
authors.forename: Chantal
authors.initials: C
authors.lastname: Demandre
authors.forename: Emile
authors.initials: E
authors.lastname: Miginiac
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Rona
authors.forename: Bruno
authors.initials: B
authors.lastname: Sotta
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Jeannette
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002478:Cells, Cultured; D011756:Diphosphates / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005990:Glycerol / Q000031:analogs & derivatives* / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D015290:Second Messenger Systems; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D011756:Diphosphates; D010712:Phosphatidic Acids; C408642:diacylglycerol pyrophosphate; C078703:rab18 protein, Arabidopsis; D000040:Abscisic Acid; D020691:rab GTP-Binding Proteins; D005990:Glycerol