Transplantation of metanephroi to sites within the abdominal cavity.
A novel approach to circumventing the shortage in transplantable donor organs is the use of embryonic primordia that develop inside the host. Previously published work has shown that transplantation of rat fetal kidney primordia (metanephroi) onto the omentum of adult rat hosts results in growth and development of the metanephroi into functioning kidney units capable of providing a measurable renal function. However, for anatomical and physiological reasons the omentum may not provide the ideal site for transplantation and may limit the maximum renal function that the transplants can achieve. We postulate that it may be possible to increase the renal function of the transplants by transplantation to sites with increased blood flow. To test this we transplanted rat embryonic day 15 metanephroi into the retroperitoneal fat adjacent to major blood vessels in the peritoneum of unilaterally nephrectomized rats; 21 days later the transplants were examined and suitable transplants connected to the host urinary system. Approximately 130 days later the glomerular filtration rate of the connected transplants was analyzed. Our results show that transplantation of metanephroi to the regions highlighted in this study results in an increased presence of urinary cysts, suggesting increased early renal function in the transplants compared to metanephroi transplanted onto the omentum, but most importantly we show that we can increase the renal function of the transplants to a level comparable with other renal therapies such as dialysis. This work suggests life-sustaining renal function could be achieved through transplantation of renal primordia.
Marshall
D
D
Intercytex Ltd, Manchester, UK.
Clancy
M
M
Bottomley
M
M
Symonds
K
K
Brenchley
P E C
PE
Bravery
C A
CA
D000005:Abdomen; D000818:Animals; D004231:Diuresis; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D005919:Glomerular Filtration Rate; D006085:Graft Survival / Q000502:physiology; D007668:Kidney / Q000196:embryology*; D016030:Kidney Transplantation / Q000379:methods*; D011247:Pregnancy; D051381:Rats; D011917:Rats, Inbred Lew; D012187:Retroperitoneal Space / Q000196:embryology / Q000601:surgery