title: Does the diagnosis of primary biliary cirrhosis or autoimmune cholangitis depend on the 'phase' of the disease?
abstract: BACKGROUND
  It is unclear whether autoimmune cholangitis (AIC) is a separate disease entity or primary biliary cirrhosis (PBC) without antimitochondrial antibodies (AMA) since fluctuation of AMA titres by immunofluorescence (IF) is often observed during the course of PBC. The aim of this study was to determine the serial changes in AMA profiles during the course of initially diagnosed PBC or AIC.
  
  
  METHODS
  In this prospective study, 32 patients with PBC or AIC were followed-up for at least 20 months and tested for AMA by IF, enzyme-linked immunosorbent assay (ELISA), and immunoblotting (IB).
  
  
  RESULTS
  When positive AMA result was defined as 'AMA by IF positive', 'AMA by IF and/or ELISA positive', and 'AMA by IB positive', the diagnosis of PBC or AIC did not change in 78%, 91%, and 97%, respectively, throughout follow-up. However, the diagnosis changed in one patient, and three patients were diagnosed as AIC throughout follow-up, despite the use of all three assays.
  
  
  CONCLUSIONS
  Our results suggested that the diagnosis of PBC and AIC was dependent on the 'phase' of the respective disease in 22% of the patients when negative AMA result was defined as 'AMA by IF negative'. This may result in recommending IB analysis before making the diagnosis of AIC.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kadokawa
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Omagari
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ohba
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Masuda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hazama
authors.forename: Hideki
authors.initials: H
authors.lastname: Kinoshita
authors.forename: Ken
authors.initials: K
authors.lastname: Ohnita
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mizuta
authors.forename: Hajime
authors.initials: H
authors.lastname: Tanioka
authors.forename: Takeo
authors.initials: T
authors.lastname: Imanishi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology; D015153:Blotting, Western; D002761:Cholangitis / Q000175:diagnosis* / Q000276:immunology* / Q000503:physiopathology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis* / Q000503:physiopathology; D008111:Liver Function Tests; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology; D011336:Probability; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
chemical_list: D000974:Antibodies, Antinuclear