Canine distemper virus associated proliferation of canine footpad keratinocytes in vitro.
Infection of canine footpads with canine distemper virus (CDV) can result in so-called hard pad disease characterized by footpad epidermal proliferation and hyperkeratosis. Cultured canine footpad keratinocytes (CFK) were inoculated with a virulent canine distemper virus strain (A75/17-CDV) to study the effects of CDV-infection on keratinocyte proliferation. Infection was analyzed by immunohistochemistry and in situ hybridization for CDV nucleoprotein (N-protein) antigen and mRNA. CDV caused a persistent, non-cytocidal infection with spread from single cells to infection of the confluent cell layer 7 days post infection (p.i.). Absolute cell numbers were significantly higher in infected cultures compared to control cultures from day 4 until day 6 p.i. Infected cultures contained significantly more total DNA on day 5 p.i. compared to controls. Immunohistochemical investigation of proliferation markers Ki67 and BrdU demonstrated a nearly two-fold increase in numbers of positive cells on day 5 p.i. compared to controls. These findings demonstrate that canine distemper virus infection of canine footpad keratinocytes in vitro was associated with proliferation.
Engelhardt
P
P
Institut für Tierpathologie, Vetsuisse Fakultät, Universität Bern, Bern, Switzerland.
Wyder
M
M
Zurbriggen
A
A
Gröne
A
A
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D004216:Distemper / Q000473:pathology / Q000821:virology*; D004217:Distemper Virus, Canine / Q000472:pathogenicity*; D004283:Dog Diseases / Q000473:pathology / Q000821:virology*; D004285:Dogs; D005533:Foot Dermatoses / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology / Q000821:virology*; D019394:Ki-67 Antigen / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism; D012367:RNA, Viral
D000956:Antigens, Viral; D019394:Ki-67 Antigen; D009698:Nucleoproteins; D012367:RNA, Viral