Chromatographic investigation of macromolecular affinity interactions.
High-performance monolithic disk affinity chromatography was applied to the investigation of formation of complexes between (1) complementary polyriboadenylic and polyribouridylic acids, e.g. poly(A) and poly(U), respectively, (2) poly(A) and synthetic polycation poly(allylamine), pAA. Polyriboadenylic acid and poly(allylamine) were immobilized on macroporous disks (CIM disks). Quantitative parameters of affinity interactions between macromolecules were established using frontal analysis at different flow rates.
Platonova
Galina A
GA
Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg 199004, Russia.
Tennikova
Tatiana B
TB
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007700:Kinetics; D011061:Poly A / Q000737:chemistry*; D011072:Poly U / Q000737:chemistry*
D011061:Poly A; D011072:Poly U