Short communication: HIV type 1 phenotype, tropism, and sequence patterns: association and preference.
In this study, the relationship between biological phenotypes, coreceptor usages, and sequence patterns of V1V2 or V3 regions on HIV-1 envelope gp 120 was carefully analyzed based on the existing isolates in the Los Alamos National Laboratory sequence database. Obviously, SI/NSI phenotypes were closely linked to the capability of HIV-1 to use coreceptor CXCR4, but not CCR5. Moreover, compared to NSI or R5 isolates, SI or X4 HIV-1 isolates significantly prefer higher net charges and the loss of the N-linked glycosylation site in the V3 loop, but they show no preference in either net charge or N-linked glycosylation of the V1V2 region. In addition, no significant relationship between V1V2 length and virus tropism or phenotypes was observed.
Dong
Xiao-Nan
XN
Laboratory of Immunology, Research Centre for Medical Science and Department of Biology, Tsinghua University, and Protein Science Laboratory of MOE, Beijing 100084, Peoples Republic of China.
Chen
Xi
X
Chen
Yu
Y
Ablimit
Ablikim
A
Ye
Zi
Z
Wu
Yi
Y
Chen
Ying-Hua
YH
D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000737:chemistry*; D019302:HIV Envelope Protein gp160 / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D010641:Phenotype; D019713:Receptors, CCR5 / Q000502:physiology; D018523:Tropism
D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D019713:Receptors, CCR5