Isolation and physical mapping of sex-linked AFLP markers in nile tilapia (Oreochromis niloticus L.).
Gynogenetically produced XX and YY Nile tilapia (Oreochromis niloticus) and diploid control groups were screened for amplified fragment length polymorphisms (AFLPs) to search for sex-linked or sex-specific markers. Family-level bulked segregant analysis (XX and YY gynogenetic family pools) and individual screening (XX and YY gynogenetics and XX and XY control individuals) identified 3 Y-linked (OniY425, OniY382, OniY227) and one X-linked (OniX420) AFLP markers. OniX420 and OniY425 were shown to be allelic. Single locus polymerase chain reaction assays were developed for these markers. Tight linkage was demonstrated between the AFLP markers and the sex locus within the source families. However, these markers failed to consistently identify sex in unrelated individuals, indicating recombination between the markers and the sex-determining loci. O. niloticus bacterial artificial chromosome clones, containing the AFLP markers, hybridized to the long arm of chromosome 1. This confirmed previous evidence, based on meiotic chromosome pairing and fluorescence in situ hybridization probes obtained through chromosome microdissection, that chromosome pair 1 is the sex chromosomes.
Ezaz
M Tariq
MT
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK.
Harvey
Simon C
SC
Boonphakdee
Chuta
C
Teale
Alan J
AJ
McAndrew
Brendan J
BJ
Penman
David J
DJ
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D023681:Cichlids / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010375:Pedigree; D020161:Physical Chromosome Mapping; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012730:Sex Chromosomes / Q000235:genetics*; D019849:Sex Determination Processes
D017931:DNA Primers; D005819:Genetic Markers