Identification, evolution, and regulation of expression of Guinea pig trappin with an unusually long transglutaminase substrate domain.
Trappins are found in human, bovine, hippopotamus, and members of the pig family, but not in rat and mouse. To clarify the evolution of the trappin genes and the functional significance of their products, we isolated the trappin gene in guinea pig, a species belonging to a rodent family distinct from rat and mouse. Guinea pig trappin was confirmed to encode the same domain structure as trappin, consisting of a signal sequence, an extra large transglutaminase substrate domain, and a whey acidic protein motif. Northern blot analysis and in situ hybridization histochemistry as well as immunohistochemistry demonstrated that guinea pig trappin is expressed solely in the secretory epithelium of the seminal vesicle and that its expression is androgen-dependent. We confirmed that guinea pig trappin is cross-linked by prostate transglutaminase and that the whey acidic protein motif derived from guinea pig trappin has an inhibitory activity against leukocyte elastase. Genome sequence analysis showed that guinea pig trappin belongs to the family of REST (rapidly evolving seminal vesicle transcribed) genes.
Furutani
Yutaka
Y
Molecular Cellular Pathology Research Unit, RIKEN, Wako-shi, Saitama 351-0198, Japan.
Kato
Akira
A
Fibriani
Azzania
A
Hirata
Taku
T
Kawai
Ryoji
R
Jeon
Ju-Hong
JH
Fujii
Yasuhisa
Y
Kim
In-Gyu
IG
Kojima
Soichi
S
Hirose
Shigehisa
S
D000595:Amino Acid Sequence; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D003432:Cross-Linking Reagents; D018076:DNA, Complementary / Q000737:chemistry; D019143:Evolution, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D006168:Guinea Pigs; D006801:Humans; D007150:Immunohistochemistry; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors; D008297:Male; D051379:Mice; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011467:Prostate / Q000201:enzymology; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D012669:Seminal Vesicles / Q000737:chemistry; D016415:Sequence Alignment; D013552:Swine; D014018:Tissue Distribution; D011503:Transglutaminases / Q000378:metabolism*
D000728:Androgens; D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D008894:Milk Proteins; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; C024471:whey acidic proteins; D011503:Transglutaminases; D019272:Leukocyte Elastase; D002118:Calcium