Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron.
A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.
Johnson
Deborah M
DM
School of Biomedical and Molecular Sciences, University of Surrey, Guildford, UK.
Yamaji
Sachie
S
Tennant
Jason
J
Srai
Surjit K
SK
Sharp
Paul A
PA
D019921:Biotinylation; D018938:Caco-2 Cells; D027682:Cation Transport Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D033862:Iron-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D000099236:Solute Carrier Family 11, Member 2; D052118:Lysosomal-Associated Membrane Protein 1
D027682:Cation Transport Proteins; D007501:Iron; D033862:Iron-Binding Proteins; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D000099236:Solute Carrier Family 11, Member 2; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1