title: Brucella spp. lumazine synthase: a novel antigen delivery system.
abstract: Lumazine synthase from Brucella spp. (BLS) was evaluated as a protein carrier to improve antigen delivery of KETc1, one of the peptides of the anti-cysticercosis vaccine. KETc1 becomes antigenic, preserved its immunogenicity and its protective capacity when expressed as a recombinant chimeric protein using Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2(d), H-2(k) nor H-2(b) haplotype-restricted albeit KETc1 is preferentially presented in the H-2(b) haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, México 04510, DF, México. edda@servidor.unam.mx
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.forename: Andrea
authors.initials: A
authors.lastname: Toledo
authors.forename: Carmen
authors.initials: C
authors.lastname: Cruz
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rosas
authors.forename: Gabriela
authors.initials: G
authors.lastname: Meneses
authors.forename: Diego
authors.initials: D
authors.lastname: Laplagne
authors.forename: Natalia
authors.initials: N
authors.lastname: Ainciart
authors.forename: Jacquelynne
authors.initials: J
authors.lastname: Cervantes
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Goldbaum
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002002:Brucella / Q000201:enzymology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009097:Multienzyme Complexes / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D041201:Taenia solium / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C044822:6,7-dimethyl-8-ribityllumazine synthase