Novel TEM-type extended-spectrum beta-lactamase, TEM-134, in a Citrobacter koseri clinical isolate.
A new natural TEM derivative with extended-spectrum beta-lactamase activity, TEM-134, was identified in a ceftazidime-resistant clinical isolate of Citrobacter koseri. Compared to TEM-1, TEM-134 contains the following mutations: Q39K, E104K, R164H, and G238S. The bla(TEM-134) gene was not transferable by conjugation and, apparently, was chromosomally encoded. Expression studies with Escherichia coli revealed efficient cefotaximase and ceftazidimase activity for TEM-134.
Perilli
Mariagrazia
M
Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, L'Aquila, Italy.
Mugnaioli
Claudia
C
Luzzaro
Francesco
F
Fiore
Marianna
M
Stefani
Stefania
S
Rossolini
Gian Maria
GM
Amicosante
Gianfranco
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D018550:Cephalosporin Resistance; D041041:Citrobacter koseri / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000145:classification* / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002442:Ceftazidime; D001618:beta-Lactamases