title: Photoprotective effect of Pothomorphe umbellata root extract against ultraviolet radiation induced chronic skin damage in the hairless mouse.
abstract: In this study we evaluated the activity of Pothomorphe umbellata root extract on hairless mice chronically exposed to UVB radiation (76.5 mJ/cm(2), 4 days per week for 22 weeks). Mouse dorsal surfaces were treated topically with 20 mg/cm(2) of a carbomer 940 gel (vehicle) with or without P. umbellata root extract to a final concentration of 0.1%, for 2 h before irradiation. Another irradiated group received no topical treatment. A fourth group received no treatment and was not irradiated. Visible skin wrinkling was evaluated using a scale ranging from 0 to 4, where 0 corresponds to no skin modification, and 4 to the maximum visual skin alteration observed in our experiments. Histological measurements were carried out on standard haematoxylin & eosin stained sections. The mean distances between the outermost surface of the epidermis (excluding the stratum corneum) and the dermal-epidermal junction were determined by morphometric analysis. These distances were statistically increased in the irradiated control groups when compared to the nonirradiated control group and to the irradiated group using P. umbellata root extract. These data demonstrate that P. umbellata may be successfully used as a topical skin-protecting agent against the deleterious effects of UV radiation.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, School of Pharmaceutical Sciences, University of São Paulo, Avenue. Lineu Prestes, 580, São Paulo-P, 05508-00, Brazil. cdropke@usp.br
authors.forename: C D
authors.initials: CD
authors.lastname: Ropke
authors.forename: T C H
authors.initials: TC
authors.lastname: Sawada
authors.forename: V V
authors.initials: VV
authors.lastname: da Silva
authors.forename: N S
authors.initials: NS
authors.lastname: Michalany
authors.forename: S B
authors.initials: SB
authors.lastname: de Moraes Barros
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D051379:Mice; D008812:Mice, Hairless; D008517:Phytotherapy / Q000379:methods*; D026321:Piperaceae; D010936:Plant Extracts / Q000627:therapeutic use; D018517:Plant Roots; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D012720:Severity of Illness Index; D015595:Skin Aging / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D010936:Plant Extracts; D011837:Radiation-Protective Agents