A 7872 cDNA microarray and its use in bovine functional genomics.
The strategy used to create and annotate a 7872 cDNA microarray from cattle placenta and spleen cDNA sequences is described. This microarray contains approximately 6300 unique genes, as determined by BLASTN and TBLASTX similarity search against the human and mouse UniGene and draft human genome sequence databases (build 34). Sequences on the array were annotated with gene ontology (GO) terms, thereby facilitating data analysis and interpretation. A total of 3244 genes were annotated with GO terms. The array is rich in sequences encoding transcription factors, signal transducers and cell cycle regulators. Current research being conducted with this array is described, and an overview of planned improvements in our microarray platform for cattle functional genomics is presented.
Everts
Robin E
RE
Department of Animal Sciences, University of Illinois, 206 Edward R. Madigan Laboratory, 1201 West Gregory Drive, Urbana, IL 61801, USA.
Band
Mark R
MR
Liu
Z Lewis
ZL
Kumar
Charu G
CG
Liu
Lei
L
Loor
Juan J
JJ
Oliveira
Rosane
R
Lewin
Harris A
HA
D000818:Animals; D002417:Cattle / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology; D020869:Gene Expression Profiling / Q000662:veterinary*; D023281:Genomics / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*