Potential ability of hot water adzuki (Vigna angularis) extracts to inhibit the adhesion, invasion, and metastasis of murine B16 melanoma cells.
The 40% ethanol eluent of the fraction of hot-water extract from adzuki beans (EtEx.40) adsorbed onto DIAION HP-20 resin has many biological activities, for example, antioxidant, antitumorigenesis, and intestinal alpha-glucosidase suppressing activities. This study examined the inhibitory effect of EtEx.40 on experimental lung metastasis and the invasion of B16-BL6 melanoma cells. EtEx.40 was found significantly to reduce the number of tumor colonies. It also inhibited the adhesion and migration of B16-BL6 melanoma cells into extracellular matrix components and their invasion into reconstituted basement membrane (matrigel) without affecting cell proliferation in vitro. These in vivo data suggest that EtEx.40 possesses a strong antimetastatic ability, which might be a lead compound in functional food development.
Itoh
Tomohiro
T
Imuraya Confectionary Co., Ltd., Tsu, Mie, Japan. chuken@imuraya.co.jp
Umekawa
Hayato
H
Furuichi
Yukio
Y
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006358:Hot Temperature; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000494:pharmacology*; D014867:Water / Q000737:chemistry*
D010936:Plant Extracts; D014867:Water