title: [Regulation of TH activity by GAD immunoreactivity in rat peri-LC after chronic variable stress].
abstract: Locus coeruleus (LC) is the major component of noradrenergic neurons in the brain. The corticotropin-releasing hormone (CRH) and norepinephrine (NE) are suggested to play a role in modulating the central stress response. In a previous study we observed a decrease of the basal level of tyrosine hydroxylase (TH) immunoreactivity (-ir) in the LC of rats treated with chronic variable stress (CVS) for 14 days. Furthermore a novel stressor produced an enhanced response of the TH-ir after CVS. In the present study we examined the effect of CVS on the glutamic acid decarboxylase (GAD)-ir activity of periaqueductal gray (PAG), prepositus hypoglossi (PrH) and peri-LC. The GAD-ir was significantly increased in PrH and peri-LC after CVS. The footshock-induced reactivity in the GAD-ir was decreased in both regions after CVS. Moreover, we investigated the influence of the CRH receptor antagonist, alphah-CRH (i.c.v.) on the CVS-induced activation of the TH-ir in the LC. The alphah-CRH i.c.v. diminished the enhanced-TH reactivity by novel stressor after CVS. Our results suggest that the GABA activity in peri-LC and PrH might regulate the LC-TH response, and also the CRH input from central nucleus of amygdala (CeA) and/or the bed nucleus of stria terminalis (BNST) might regulate the TH reactivity.
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, 216-8511, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tanaka
authors.forename: Mikio
authors.initials: M
authors.lastname: Asakura
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kanai
authors.forename: Takuji
authors.initials: T
authors.lastname: Hishinuma
authors.forename: Sachiko
authors.initials: S
authors.lastname: Fujii
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000201:enzymology*; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology*; D010487:Periaqueductal Gray / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D013315:Stress, Psychological / Q000503:physiopathology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D018019:Receptors, Corticotropin-Releasing Hormone; C069206:alpha helical corticotropin-releasing hormone; D005680:gamma-Aminobutyric Acid; D003346:Corticotropin-Releasing Hormone; D005968:Glutamate Decarboxylase