title: Regional abnormalities in retinal development are associated with local ocular hypopigmentation.
abstract: The retinal pigment epithelium (RPE) plays a key role in regulating retinal development. The critical enzyme in pigment production is tyrosinase. Transgenic mice with a tyrosinase construct where the locus control region was deleted (YRT4) display a variegated phenotype of tyrosinase expression. Their central retina is largely pigment free, whereas more peripheral regions are heavily pigmented. We have used this model to ask whether the influence of pigmented RPE over the retina during development is fundamentally governed by local interactions or is global. Our data show that YRT4 eyes have intermediate melanin content and relatively low tyrosinase activity compared with wild-type and albino animals. Rod counts are comparable to those in pigmented mice in peripheral regions but similar to those in albinos centrally. Anterograde labelling of retinal pathways demonstrates the presence of relatively normal ipsilateral chiasmatic projection in YRT4 mice, comparable with that in pigmented animals and consistent with the peripheral pigmented origin of this pathway. Examination of cellular proliferation levels during retinal development reveals that YRT4 mice display an extended period of mitosis, similar to that found in albinos. Hence, our results show that the regulatory influence of the RPE over the developing retina depends on localized interactions between these tissues.
authors.affiliation: Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, Campus de Cantoblanco, C/Darwin 3, 28049 Madrid, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.forename: Alfonso
authors.initials: A
authors.lastname: Lavado
authors.forename: Glen
authors.initials: G
authors.lastname: Jeffery
authors.forename: Lluís
authors.initials: L
authors.lastname: Montoliu
mesh_terms: D016117:Albinism, Ocular / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017496:Hypopigmentation / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D014442:Monophenol Monooxygenase / Q000096:biosynthesis / Q000235:genetics; D010857:Pigment Epithelium of Eye / Q000002:abnormalities* / Q000201:enzymology / Q000378:metabolism; D012160:Retina / Q000002:abnormalities* / Q000378:metabolism
chemical_list: D008543:Melanins; D014442:Monophenol Monooxygenase