Caspase-8 sumoylation is associated with nuclear localization.
The cysteine protease caspase-8 plays a pivotal role in the initiation of different apoptotic pathways and controls the maturation and differentiation of various cell types including neurons, fibroblasts and lymphocytes. Specific substrates of caspase-8 are present in both the cytoplasm and the nucleus, which may determine the ultimate biological effect of caspase-8. However, the mechanisms regulating the cellular localization of caspase-8 are still unknown. We show here that, in contrast to other caspases such as caspase-9 and -3, caspase-8 can be sumoylated at lysine 156. This sumoylation (i) is associated with the nuclear localization of caspase-8 and (ii) did not impair caspase-8 activation.
Besnault-Mascard
Laurence
L
INSERM U542, Hôpital Paul Brousse, Bâtiment Lavoisier, 14 Avenue Paul Vaillant Couturier, 94807 Villejuif cedex, France.
Leprince
Corinne
C
Auffredou
Marie Thérèse
MT
Meunier
Brigitte
B
Bourgeade
Marie Françoise
MF
Camonis
Jacques
J
Lorenzo
Hans Kristian
HK
Vazquez
Aimé
A
D000595:Amino Acid Sequence; D000841:Anisomycin / Q000494:pharmacology; D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D019169:Jurkat Cells; D008214:Lymphocytes / Q000378:metabolism; D008239:Lysine / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D025842:SUMO-1 Protein / Q000378:metabolism / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D019384:Nucleic Acid Synthesis Inhibitors; D025842:SUMO-1 Protein; D000841:Anisomycin; C505407:CASP3 protein, human; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D008239:Lysine; D003545:Cysteine