title: Vascular corrosion casts mirroring early morphological changes that lead to the formation of saccular cerebral aneurysm: an experimental study in rats.
abstract: OBJECTIVE
  The formation of cerebral aneurysms involves complex processes and little is known about the mechanisms by which they originate, grow, and rupture. The purpose of this study was to identify early ultrastructural morphological changes that lead to the formation of experimental cerebral aneurysms.
  
  
  METHODS
  Twenty male Sprague-Dawley rats were subjected to cerebral aneurysm induction (renal hypertension and right common carotid artery ligation); 10 intact rats served as the control group. The animals were killed after 2 months, and a vascular corrosion cast of their cerebral arteries was prepared and screened for aneurysm development by using a scanning electron microscope. Sequential morphological changes observed at the cerebral artery bifurcation in response to hemodynamic shear stress included endothelial changes, intimal pad elevation, and saccular dilation. Endothelial cell changes were the first observed morphological changes; they were followed by various degrees of artery wall dilation. No aneurysmal changes developed in any of the control rats. Of the 20 surgically treated rats, 11 displayed aneurysmal changes. In five of these animals only changes in the endothelial cell imprints could be identified. In the other six rats morphological changes in endothelial cells were associated with different stages of aneurysmal dilation.
  
  
  CONCLUSIONS
  This is the first study to demonstrate in vivo early morphological changes that lead to the formation of cerebral aneurysms. The morphological findings indicate the principal role of endothelial cells in the pathogenesis of cerebral aneurysms and suggest that hemodynamic shear stress and blood flow patterns may precipitate these early changes.
authors.affiliation: Department of Neurosurgery, School of Medicine, The Institute of Health Bioscience, The University of Tokushima, Tokushima City, Japan. neuros@clin.med.tokushima-u.ac.jp
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Jamous
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagahiro
authors.forename: Keiko T
authors.initials: KT
authors.lastname: Kitazato
authors.forename: Koichi
authors.initials: K
authors.lastname: Satoh
authors.forename: Junichiro
authors.initials: J
authors.lastname: Satomi
mesh_terms: D000818:Animals; D016319:Corrosion Casting; D002532:Intracranial Aneurysm / Q000473:pathology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical