Brucella spp. lumazine synthase: a novel antigen delivery system.
Lumazine synthase from Brucella spp. (BLS) was evaluated as a protein carrier to improve antigen delivery of KETc1, one of the peptides of the anti-cysticercosis vaccine. KETc1 becomes antigenic, preserved its immunogenicity and its protective capacity when expressed as a recombinant chimeric protein using Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2(d), H-2(k) nor H-2(b) haplotype-restricted albeit KETc1 is preferentially presented in the H-2(b) haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.
Sciutto
Edda
E
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, México 04510, DF, México. edda@servidor.unam.mx
Toledo
Andrea
A
Cruz
Carmen
C
Rosas
Gabriela
G
Meneses
Gabriela
G
Laplagne
Diego
D
Ainciart
Natalia
N
Cervantes
Jacquelynne
J
Fragoso
Gladis
G
Goldbaum
Fernando A
FA
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002002:Brucella / Q000201:enzymology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009097:Multienzyme Complexes / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D041201:Taenia solium / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D000947:Antigens, Helminth; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C044822:6,7-dimethyl-8-ribityllumazine synthase