title: Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.
abstract: Interactions between proteins are essential for their functioning and the biological processes they control. The elucidation of interaction maps based on yeast studies is a first step toward the understanding of molecular networks and provides a framework of proteins that possess the capacity and specificity to interact. Here, we present a comprehensive plant protein-protein interactome map of nearly all members of the Arabidopsis thaliana MADS box transcription factor family. A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers. Clustering of proteins with similar interaction patterns pinpoints proteins involved in the same developmental program and provides valuable information about the participation of uncharacterized proteins in these programs. Furthermore, a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved. Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower.
authors.affiliation: Business Unit Bioscience, Plant Research International, 6700 AA Wageningen, The Netherlands.
authors.forename: Stefan
authors.initials: S
authors.lastname: de Folter
authors.forename: Richard G H
authors.initials: RG
authors.lastname: Immink
authors.forename: Martin
authors.initials: M
authors.lastname: Kieffer
authors.forename: Lucie
authors.initials: L
authors.lastname: Parenicová
authors.forename: Stefan R
authors.initials: SR
authors.lastname: Henz
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.forename: Marco
authors.initials: M
authors.lastname: Busscher
authors.forename: Maarten
authors.initials: M
authors.lastname: Kooiker
authors.forename: Lucia
authors.initials: L
authors.lastname: Colombo
authors.forename: Martin M
authors.initials: MM
authors.lastname: Kater
authors.forename: Brendan
authors.initials: B
authors.lastname: Davies
authors.forename: Gerco C
authors.initials: GC
authors.lastname: Angenent
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods; D019281:Dimerization; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances; D010802:Phylogeny; D040901:Proteomics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins; D046911:Macromolecular Substances; D014157:Transcription Factors