title: [A study on the chondrogenesis of the compound of alginate gelatin and bone marrow stromal cells in vivo].
abstract: OBJECTIVE
  To investigate the chondrogenisis by alginate gelatin and rats' bone marrow stromal cells (BMSCs) chondrogenicly induced in vitro.
  
  
  METHODS
  Thirty-two male adult SD rats were assigned randomly to experimental and control groups. In experimental group, bone marrow was obtained from the right tibias of all the rats. After expanding and culturing 3 passages, induced BMSCs by chondrogenic culture medium for 10 days. Suspended induced cells in alginate gelatin, and injected the complex into the hypodermic tissue of the backs of rats autogenously. In control group only alginate gelatins were injected. The grafts were taken out for examinations 4 and 8 weeks after the operations.
  
  
  RESULTS
  Considerable cartilage appeared in experimental group 8 weeks after operations. Regular HE staining and alcian blue staining showed a great deal of cartilage holding chondrocyte masses surrounded by abundant matrix. Alginate gelatin decompounded obviously, and the rest distributed among newly formed cartilage. No cartilage appeared in control group all through.
  
  
  CONCLUSIONS
  BMSCs and alginate gelatin have a beautiful future in cartilage tissue engineering.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Rurt-liang
authors.initials: RL
authors.lastname: Chen
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Tian
authors.forename: Zheng-bin
authors.initials: ZB
authors.lastname: Yan
authors.forename: Xi-zhe
authors.initials: XZ
authors.lastname: Chen
authors.forename: Sheng-wei
authors.initials: SW
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D000464:Alginates; D000818:Animals; D020219:Chondrogenesis; D005780:Gelatin; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008297:Male; D059630:Mesenchymal Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin