Anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia/reperfusion injury in rats.
The objective of this study is to investigate the anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia injury in rats and to explore its mechanisms of action. After 24 h of reperfusion following 2 h of cerebral ischemia, the infiltration of neutrophils was observed by myeloperoxidase (MPO) activity determination, the expression of intercellular adhesion molecule-1 (ICAM-1) was observed by western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, and the nuclear translocation and DNA binding activity of nuclear factor-kappaB (NF-kappaB) were observed by western blot and electrophoretic mobility shift assay (EMSA). The results showed that hydroxyethylpuerarin could obviously inhibit the MPO activity and ICAM-1 expression following 2 hours of ischemia with 24 hours of reperfusion. The nuclear translocation and DNA binding activity were also decreased by hydroxyethylpuerarin treatment. These results suggested that hydroxyethylpuerarin could inhibit neutrophil-mediated inflammatory response after brain ischemia reperfusion in rats. This effect may be mediated by down-regulation of ICAM-1 and NF-kappaB activity.
Lou
Hai-Yan
HY
Department of Pharmacology, School of Medicine, Shandong University, Jinan, Shandong 250012, PR China.
Zhang
Xiu-Mei
XM
Wei
Xin-Bing
XB
Wang
Ru-Xia
RX
Sun
Xia
X
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology / Q000473:pathology; D001923:Brain Chemistry / Q000187:drug effects; D002545:Brain Ischemia / Q000473:pathology*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D016328:NF-kappa B / Q000096:biosynthesis; D018696:Neuroprotective Agents; D016759:Oncogene Protein p65(gag-jun) / Q000096:biosynthesis; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000893:Anti-Inflammatory Agents; D007529:Isoflavones; D016328:NF-kappa B; D018696:Neuroprotective Agents; D016759:Oncogene Protein p65(gag-jun); D018799:Intercellular Adhesion Molecule-1; D004247:DNA; D009195:Peroxidase