[Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats].
OBJECTIVE
To investigate the influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats, so as to provide a theoretical basis for the clinical use of hyperoxic fluid.


METHODS
Sprague-Dawley (SD) rats with 35% TBSA full-thickness scald were randomly divided into scald control (S, n = 6,with intraperitoneal fluid infusion after scalding), hyperoxic (H, n = 6, with hyperoxic fluid infusion after scalding) groups. Six rats without scald injury served as normal group. The proteins in the intestinal mucosa were separated with the two-dimensional electrophoresis (2-DE), and were analyzed with ImageMaster 2D Elite. The influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats was studied with bio-spectrum, protein bank and document analysis.


RESULTS
(1) Among the 34 down-regulated protein spots in S group, 9 of them definitely exhibited up-regulation compared with those in H group. (2) The expression of mitochondrial aconitase, alpha-propionyl-CoA carboxylase, short chain of hydroxyacyl-Coenzyme A dehydrogenase, transcription factor EB (estradiol benzoate), triosephosphate isomerase 1, T cell receptor V delta 6, and dynein-like protein-5 in H group were significantly up-regulated.


CONCLUSIONS
The hyperoxic fluid could up-regulate the down-regulated proteins in rat intestinal mucosa at early postburn stage, so that the barrier function of intestinal mucosa of rats with severe burns could be partially recovered.
Wang
Xiao-Jun
XJ
General Surgery Department, The Sixth Municipal Hospital of Beijing, Beijing 100007, P.R. China.
Sun
Yong-Hua
YH
Wang
Jian-Ning
JN
Ding
Qin-Xue
QX
Zhang
Li-Ping
LP
Song
Guo-Cheng
GC
Xiao
Hong
H
D000818:Animals; D002056:Burns / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D011506:Proteins; D010100:Oxygen