title: The influence of biosurfactants released by S. mitis BMS on the adhesion of pioneer strains and cariogenic bacteria.
abstract: The influence of Streptococcus mitis BMS biosurfactants on the adhesion of eight pioneer and four cariogenic oral bacterial strains was, for a first screening, examined in a microtiter plate assay. The adhesion to pellicle-coated wells of three cariogenic strains was inhibited >70% by the biosurfactants, while only one pioneer strain showed >70% reduction. The reduction for the other strains did not exceed 50%. Subsequently, adhesion of Streptococcus mutans ATCC 25175 and Streptococcus sobrinus HG 1025, both cariogenic strains, and Actinomyces naeslundii T14V-J1 and Streptococcus oralis J22, two pioneer strains, to biosurfactants-coated enamel with and without a salivary pellicle was studied in a parallel plate flow chamber. A biosurfactants coating to enamel with or without a pellicle caused a reduction in the number of adhering cariogenic organisms, although no such reduction was observed for the pioneer strains. Consequently, it is concluded that S. mitis BMS biosurfactants may play a protective role against adhesion of cariogenic bacteria.
authors.affiliation: Department of Biomedical Engineering, University of Groningen, Groningen, The Netherlands. C.G.van.Hoogmoed@med.rug.nl
authors.forename: Chris G
authors.initials: CG
authors.lastname: van Hoogmoed
authors.forename: Henny C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D001422:Bacterial Adhesion; D003731:Dental Caries / Q000382:microbiology; D003743:Dental Enamel / Q000382:microbiology*; D006801:Humans; D011157:Population Dynamics; D034361:Streptococcus mitis / Q000502:physiology*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents