Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae.
A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated both metabolically and hierarchically. The method is expounded in terms of its implications for diverse types of regulation. It is also fine-tuned for cases where isoenzymes catalyze the flux through a single metabolic step.
Rossell
Sergio
S
Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, The Netherlands.
van der Weijden
Coen C
CC
Kruckeberg
Arthur L
AL
Bakker
Barbara M
BM
Westerhoff
Hans V
HV
D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D023281:Genomics; D005947:Glucose / Q000378:metabolism*; D018869:Heat-Shock Response; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose; D009584:Nitrogen