Morning glory systemically accumulates scopoletin and scopolin after interaction with Fusarium oxysporum.
An isolate of non-pathogenic Fusarium, Fusarium oxysporum 101-2 (NPF), induces resistance in the cuttings of morning glory against Fusarium wilt caused by F. oxysporum f. sp. batatas O-17 (PF). The effect of NPF on phenylpropanoid metabolism in morning glory cuttings was studied. It was found that morning glory tissues responded to treatment with NPF bud-cell suspension (108 bud-cells/ml) with the activation of phenylalanine ammonia-lyase (PAL). PAL activity was induced faster and greater in the NPF-treated cuttings compared to cuttings of a distilled water control. High performance liquid chromatography analysis of the extract from tissues of morning glory cuttings after NPF treatment showed a quicker induction of scopoletin and scopolin synthesis than that seen in the control cuttings. PF also the induced synthesis of these compounds at 10(5) bud-cells/ml, but inhibited it at 10(8) bud-cells/ml. Possibly PF produced constituent(s) that elicited the inhibitory effect on induction of the resistance reaction. These compounds could potentially be useful as markers to detect early beginning interactions between Fusarium and morning glory tissues cuttings.
Shimizu
Bun-ichi
B
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. bun@biofun.kuicr.kyoto-u.ac.jp
Miyagawa
Hisashi
H
Ueno
Tamio
T
Sakata
Kanzo
K
Watanabe
Ken
K
Ogawa
Kei
K
D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism / Q000382:microbiology; D005670:Fusarium / Q000502:physiology*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D027741:Ipomoea / Q000378:metabolism* / Q000382:microbiology*; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D012603:Scopoletin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D003374:Coumarins; D005960:Glucosides; C417572:scopolin; D012603:Scopoletin