title: Ex vivo amplification of T cells from human cord blood.
abstract: The current status of immunotherapy for cancer is here summarized with particular attention to the new methodologies developed for ex vivo expansion of T cells from neonatal cord blood. Umbilical cord blood (UCB) mononuclear cells (MNC) generate CD45RA naive T lymphocytes when cultured under serum-deprived conditions with appropriate combinations of growth factors. These ex vivo generated T cells resemble precursors for the lymphoid lineage present in adult bone marrow in terms of active transcription of RAG-2 and pTalpha.
authors.affiliation: Laboratory of Clinical Biochemistry, Istituto Superiore di Sanità, Rome, Italy. migliagi@iss.it
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Migliaccio
authors.forename: Elena
authors.initials: E
authors.lastname: Alfani
authors.forename: Viviana
authors.initials: V
authors.lastname: Di Giacomo
authors.forename: Monia
authors.initials: M
authors.lastname: Cieri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Migliaccio
mesh_terms: D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood; D001854:Bone Marrow Cells / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D006133:Growth Substances