title: Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles.
abstract: Signaling by G protein-coupled receptors coupled to Galpha(i) assists in triggering lymphocyte movement into and out of lymph nodes. Here, we show that modulating the signaling output from these receptors dramatically alters B cell trafficking. Intravital microscopy of adoptively transferred B cells from wild-type and Rgs1-/- mice revealed that Rgs1-/- B cells stick better to lymph node high endothelial venules, home better to lymph nodes, and move more rapidly within lymph node follicles than do wild-type B cells. In contrast, B cells from Gnai2-/- mice enter lymph nodes poorly and move more slowly than do wild-type B cells. The Gnai2-/- mice often lack multiple peripheral lymph nodes, and their B cells respond poorly to chemokines, indicating that Galpha(i1) and Galpha(i3) poorly compensate for the loss of Galpha(i2). These results demonstrate opposing roles for Rgs1 and Gnai2 in B cell trafficking into and within lymph nodes.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious, Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Chantal
authors.initials: C
authors.lastname: Moratz
authors.forename: Ning-Na
authors.initials: NN
authors.lastname: Huang
authors.forename: Brian
authors.initials: B
authors.lastname: Kelsall
authors.forename: Hyeseon
authors.initials: H
authors.lastname: Cho
authors.forename: Chong-Shan
authors.initials: CS
authors.lastname: Shi
authors.forename: Owen
authors.initials: O
authors.lastname: Schwartz
authors.forename: John H
authors.initials: JH
authors.lastname: Kehrl
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000276:immunology*; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D020710:RGS Proteins / Q000276:immunology*
chemical_list: D011518:Proto-Oncogene Proteins; D020710:RGS Proteins; C488188:Rgs1 protein, mouse; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C498016:Gnai2 protein, mouse