title: Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae).
abstract: Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (approximately 37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.
authors.affiliation: Cell Engineering & 3-D Structure Laboratory, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Jai-Hoon
authors.initials: JH
authors.lastname: Eum
authors.forename: Sung-Moon
authors.initials: SM
authors.lastname: Yoe
authors.forename: Young-R
authors.initials: YR
authors.lastname: Seo
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Han
mesh_terms: D000818:Animals; D001483:Base Sequence; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D007313:Insecta / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D019476:Insect Proteins