SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella.
Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. This phenotype is dependent upon clg, a gene encoding a collagenase in Salmonella. Using a novel transposon system and an RT-PCR assay for detection of clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on clg expression was restricted to DT104 and other Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component.
Carlson
Steve A
SA
Preharvest Food Safety and Enteric Disease Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Road, Box 70, Ames, IA 50010, USA. scarlson@nadc.ars.usda.gov
McCuddin
Zoe P
ZP
Wu
Max T
MT
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002460:Cell Line; D017364:Collagenases / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D032023:Integrons; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012329:RNA, Bacterial; D014157:Transcription Factors; D017364:Collagenases