Effects of selected flavonoids and carotenoids on drug accumulation and apoptosis induction in multidrug-resistant colon cancer cells expressing MDR1/LRP.
The effects of various flavonoids and carotenoids on Rhodamine 123 accumulation in multidrug-resistant Colo 320 human colon cancer cells expressing MDR1/LRP were studied. The Colo 205 cell line was used as a drug-sensitive control. Rotenon, Catechin, Neohesperidin, Naringin, Robinin, Phloridzin, Robinetin, Dihydrobinetin, Dihydrofisetin, Kampferol, Dihidroquercetin, Sakuranin and Sakuranetin were tested on Colo 320 cells: only Rotenon was found to be effective as regards multidrug resistance (MDR) reversal, while a majority of the flavonoids, such as Catechin, Neohesperidin, Naringin, Robinin, Phloridzin, Dihydrobinetin and Sakuranetin, had only marginal effects on Rhodamine 123 accumulation. The tested carotenoids (beta-Cryptoxanthin, Luteoxanthin, Anteroxanthin, Violeoxanthin, Apple peel fetoxanthin, Lutein, Violaxanthin and Neoxanthin) were able to increase Rhodamine 123 accumulation in Colo 320 cells. Verapamil was applied as a resistance-modifying positive control. The levels of apoptosis induction in drug-resistant and sensitive cell lines were also compared. The results indicated that the tested flavonoids were weak apoptosis inducers on MDR and parent cells, without significant differences. A majority of the carotenoids induced only early apoptosis, but apoptosis and cell death were not induced in MDR colon cancer cells.
Ugocsai
Katalin
K
Department of Medical Microbiology and Immunobiology, Faculty of General Medicine, University of Szeged, Hungary.
Varga
Andreas
A
Molnár
Péter
P
Antus
Sándor
S
Molnár
Joseph
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002338:Carotenoids / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D020112:Rhodamine 123 / Q000378:metabolism; D020394:Vault Ribonucleoprotein Particles / Q000096:biosynthesis*; D000099187:Major Vault Protein
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D002338:Carotenoids; D005419:Flavonoids; D009363:Neoplasm Proteins; D020112:Rhodamine 123; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein