The CFTR gene and regulation of its expression.
The cystic fibrosis transmembrane conductance regulator gene (CFTR) shows clear temporal and developmental regulation of its expression. However, there are few well-defined regulatory elements that control this pattern of expression, and their mechanism of action is poorly understood. We review the structure and organization of the CFTR gene and what is known about its regulation. The CFTR gene promoter is clearly important for maintaining levels of CFTR gene expression, but apparently it does not contain any tissue-specific elements. Thus tissue-specificity is probably controlled by sequences lying elsewhere in this large gene. We discuss data from our group and others implicating additional regions of CFTR in regulatory functions, and evaluate candidate transcription factors that may be involved. Further, we summarize aspects of the regulation of the developmental expression of CFTR. Definition of CFTR gene regulatory elements could be of considerable therapeutic significance, since only a small increase in CFTR expression in the correct cell type could alleviate the disease phenotype.
McCarthy
Victoria A
VA
Paediatric Molecular Genetics, Weatherall Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Oxford, UK.
Harris
Ann
A
D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D014158:Transcription, Genetic / Q000235:genetics
D022762:CCAAT-Enhancer-Binding Proteins; D006728:Hormones; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D003851:Deoxyribonucleases