Identification of putative sulfurtransferase genes in the extremophilic Acidithiobacillus ferrooxidans ATCC 23270 genome: structural and functional characterization of the proteins.
Eight nucleotide sequences containing a single rhodanese domain were found in the Acidithiobacillus ferrooxidans ATCC 23270 genome: p11, p14, p14.3, p15, p16, p16.2, p21, and p28. Amino acids sequence comparisons allowed us to identify the potentially catalytic Cys residues and other highly conserved rhodanese family features in all eight proteins. The genomic contexts of some of the rhodanese-like genes and the determination of their expression at the mRNA level by using macroarrays suggested their implication in sulfur oxidation and metabolism, formation of Fe-S clusters or detoxification mechanisms. Several of the putative rhodanese genes were successfully isolated, cloned and overexpressed in E. coli and their thiosulfate:cyanide sulfurtransferase (TST) and 3-mercaptopyruvate/cyanide sulfurtransferase (MST) activities were determined. Based on their sulfurtransferase activities and on structural comparisons of catalytic sites and electrostatic potentials between homology- modeled A. ferrooxidans rhodaneses and the reported crystal structures of E. coli GlpE (TST) and SseA (MST) proteins, two of the rhodanese-like proteins (P15 and P16.2) could clearly be defined as TSTs, and P14 and P16 could possibly correspond to MSTs. Nevertheless, several of the eight A. ferrooxidans rhodanese-like proteins may have some different functional activities yet to be discovered.
Acosta
Mauricio
M
Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
Beard
Simon
S
Ponce
Jose
J
Vera
Mario
M
Mobarec
Juan C
JC
Jerez
Carlos A
CA
D042763:Acidithiobacillus / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001665:Binding Sites; D020134:Catalytic Domain; D002474:Cell-Free System; D003001:Cloning, Molecular; D019295:Computational Biology; D018360:Crystallography, X-Ray; D003486:Cyanides / Q000378:metabolism; D003545:Cysteine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D016680:Genome, Bacterial; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010100:Oxygen / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D055672:Static Electricity; D015238:Sulfotransferases / Q000235:genetics*; D013884:Thiosulfate Sulfurtransferase / Q000235:genetics / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism
D003486:Cyanides; D017931:DNA Primers; D007506:Iron-Sulfur Proteins; D012333:RNA, Messenger; D013885:Thiosulfates; C008366:3-mercaptopyruvic acid; D013884:Thiosulfate Sulfurtransferase; D015238:Sulfotransferases; D003545:Cysteine; D010100:Oxygen