Simultaneous determination of ranitidine and metronidazole in human plasma using high performance liquid chromatography with diode array detection.
The development and validation of a simple method for the simultaneous determination of ranitidine and metronidazole in human plasma is described. Plasma samples (250 microL) were deproteinized by precipitation with 60% perchloric acid, centrifuged and the supernatant directly injected into the HPLC. Separation was achieved in isocratic mode with a Shimpak C(18) column and a mobile phase consisting of 10mM potassium dihydrogen phosphate pH 3.5:acetonitrile (90:10, v/v) with UV detection at 315 nm. The method showed good selectivity and sensitivity. Good and consistent recovery for metronidazole and ranitidine was obtained: 96.22+/-3.52 and 95.00+/-4.50% for ranitidine (25-1000 ng/mL) and metronidazole (60-10,000 ng/mL), respectively (n=3). With this one-step sample preparation method, both ranitidine and metronidazole could be quantified simultaneously in human plasma with good precision (R.S.D.<15%) and accuracy (bias values below 15%). The limit of quantification for ranitidine and metronidazole were 20 and 40 ng/mL plasma, respectively.
do Nascimento
Ticiano Gomes
TG
Unidade de Desenvolvimento e Ensaios de Medicamentos (UDEM/ LTF), Universidade Federal da Paraíba-UFPB, Campus I, Cidade Universitária, 58051-970 João Pessoa, Paraíba, Brazil.
Oliveira
Eduardo de Jesus
Ede J
Macêdo
Rui Oliveira
RO
D000890:Anti-Infective Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006635:Histamine H2 Antagonists / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D008795:Metronidazole / Q000097:blood*; D010472:Perchlorates; D011899:Ranitidine / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012972:Sodium Hydroxide; D012997:Solvents; D013048:Specimen Handling; D013395:Sucrose; D013696:Temperature
D000890:Anti-Infective Agents; D006635:Histamine H2 Antagonists; D007202:Indicators and Reagents; D010472:Perchlorates; D012997:Solvents; D008795:Metronidazole; D000431:Ethanol; D012972:Sodium Hydroxide; D013395:Sucrose; D011899:Ranitidine