title: Molecular monitoring of the developmental bacterial community in the gastrointestinal tract of Japanese infants.
abstract: The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.
authors.affiliation: Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Prapa
authors.initials: P
authors.lastname: Songjinda
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kuroki
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Tanaka
authors.forename: Sanae
authors.initials: S
authors.lastname: Fukuda
authors.forename: Chikako
authors.initials: C
authors.lastname: Kiyohara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Kunio
authors.initials: K
authors.lastname: Izuchi
authors.forename: Taro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S