title: Vitamin A deficiency alters the structure and collagen IV composition of rat renal basement membranes.
abstract: Retinoids can modulate the expression of extracellular matrix (ECM) proteins with variable results depending on other contributing factors. Because changes in these proteins may alter the composition and impair the function of specialized ECM structures such as basement membranes (BMs), we studied the effects of vitamin A deficiency on renal BMs during the growing period. Newborn male rats were fed a vitamin A-deficient (VAD) diet for 50 d. The ultrastructure of renal BMs was analyzed by electron microscopy. Total collagen IV, the different alpha(IV) chains, matrix degrading metalloproteinases (MMP), and tissue inhibitors of metalloproteinases (TIMP) were quantified by immunocytochemistry and/or Western blotting. Tumor necrosis factor-alpha and interleukin-1beta were measured by ELISA. Semiquantitative RT-PCR was used for determining the steady-state levels for each alpha(IV) chain mRNA. VAD renal BMs showed an irregular thickening, particularly tubular BM. The total collagen IV content was increased, but there was a differential expression of the collagen IV chains. The protein amounts for alpha1(IV), alpha4(IV), and alpha5(IV) were similarly increased, whereas alpha2(IV) and alpha3(IV) were decreased. The levels of mRNA for each collagen IV chain changed in parallel with those of the corresponding protein. Both MMP2 and MMP9 were diminished, but no change was detected in TIMP1 or TIMP2. Our data indicate that nutritional VAD leads to alterations in the structure of renal BMs and to quantitative and qualitative variations in its collagen IV composition. These changes may be a factor predisposing to or resulting in kidney malfunction and renal disease.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Marín
authors.forename: Guillermo
authors.initials: G
authors.lastname: Esteban-Pretel
authors.forename: Ruth
authors.initials: R
authors.lastname: Alonso
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sado
authors.forename: Teresa
authors.initials: T
authors.lastname: Barber
authors.forename: Jaime
authors.initials: J
authors.lastname: Renau-Piqueras
authors.forename: Joaquín
authors.initials: J
authors.lastname: Timoneda
mesh_terms: D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017931:DNA Primers; D005260:Female; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014802:Vitamin A Deficiency / Q000378:metabolism* / Q000473:pathology
chemical_list: D024141:Collagen Type IV; D017931:DNA Primers; D012333:RNA, Messenger; D020782:Matrix Metalloproteinases