title: Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways.
abstract: Agonists of the dopamine receptors have been demonstrated to have bronchodilatory properties in pathologically constricted airways. The mechanism by which these agonists induce bronchodilatation is thought to involve airway sensory nerves. In this study, the expression and function of dopamine D(2) receptor were examined in sensory ganglia supplying the airways. Neuronal dopamine D(2) receptor mRNA expression was demonstrated by single-cell RT-PCR following laser-assisted microdissection. The projection of the neurons to the airways was confirmed by retrograde neuronal labeling. In functional studies, dopamine D(2) receptor agonists (AR-C65116AB and ropinirole) inhibited intraneuronal calcium mobilization in rat capsaicin-sensitive primary sensory neurons and capsaicin-induced plasma extravasation in the rat trachea. Our results provide support to the hypothesis that dopamine D(2) receptor activation inhibits neurogenic inflammation and proinflammatory reflex responses.
authors.affiliation: Department of Pediatric Pneumology and Immunology, Charité- Virchow Campus, Berlin, Germany. christian.peiser@charite.de
authors.forename: Christian
authors.initials: C
authors.lastname: Peiser
authors.forename: Marcello
authors.initials: M
authors.lastname: Trevisani
authors.forename: David A
authors.initials: DA
authors.lastname: Groneberg
authors.forename: Q Thai
authors.initials: QT
authors.lastname: Dinh
authors.forename: Doerthe
authors.initials: D
authors.lastname: Lencer
authors.forename: Silvia
authors.initials: S
authors.lastname: Amadesi
authors.forename: Barbara
authors.initials: B
authors.lastname: Maggiore
authors.forename: Selena
authors.initials: S
authors.lastname: Harrison
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Geppetti
authors.forename: Axel
authors.initials: A
authors.lastname: Fischer
mesh_terms: D000402:Airway Obstruction / Q000503:physiopathology; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology; D018491:Dopamine Agonists / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007249:Inflammation / Q000378:metabolism; D008297:Male; D009620:Nodose Ganglion / Q000166:cytology / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000096:biosynthesis*; D011955:Receptors, Drug / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*
chemical_list: D018491:Dopamine Agonists; D007211:Indoles; D017448:Receptors, Dopamine D2; D011955:Receptors, Drug; C046649:ropinirole; D002211:Capsaicin