title: A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum.
abstract: In the search for immunohistochemical markers of the developing human brain, a monoclonal antibody, HFB-16, was raised against homogenates from the cerebrum of a 15-gestational-week-old (GW) human fetus and screened on paraffin-embedded human embryonic brain specimens. This antibody was particularly useful as a marker for Purkinje cells in the developing human cerebellum. Positive immunoreactivities with HFB-16 first appeared in the Purkinje cell layer at 17 GW. From 20 to 24 GW, positive immunoreactivities were found above the lamina dissecans. After 25 GW, dendrites of Purkinje cells were found with the HFB-16 antibody, and the nerve fibers of the Purkinje cells became positive after 35 GW. Neurons in the dentate nucleus and external and internal granular layers reacted negatively to this antibody. After 1 year, when the external granular layer faded out, the dendrites of the Purkinje cells reached the pial surface of the cerebellum, and nerve fibers began to develop in the white matter. This antibody was also useful for characterization of components in heterotopic neurons found in various anomaly syndromes such as trisomy 13. Expressional cloning indicated the antigen against HFB-16 to be human KIAA0864 protein, which is supposed to be an alternative splicing product of p116Rip, whose function has not yet been elucidated. The antigenicity of the KIAA0864 protein was confirmed using human cDNA of the KIAA0864 protein, a protein expression vector, and an HFB-16 antibody.
authors.affiliation: Department of Pathology, St. Mary's Hospital, 422, Tsubukuhon-machi, Kurume-shi, Japan 830-8543. naka@st-mary-med.or.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Munehiko
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Eriko
authors.initials: E
authors.lastname: Oda
authors.forename: Yonehiro
authors.initials: Y
authors.lastname: Kanemura
authors.forename: Eri
authors.initials: E
authors.lastname: Kodama
authors.forename: Atsuyo
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyado
authors.forename: Hirotaka James
authors.initials: HJ
authors.lastname: Okano
authors.forename: Ryouji
authors.initials: R
authors.lastname: Fukagawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Higaki
authors.forename: Mami
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D001933:Brain Stem / Q000378:metabolism; D002531:Cerebellum / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005865:Gestational Age; D006801:Humans; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D011689:Purkinje Cells / Q000378:metabolism*; D013687:Telencephalon / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007074:Immunoglobulin G; C498552:MPRIP protein, human; D020742:rhoA GTP-Binding Protein