Syngeneic islet transplantation into seminal vesicles of diabetic rats.
Pancreatic islet transplantation has been proposed as an attractive option for the treatment of type I diabetes. Transplantation into different sites has been investigated, among them those that are immuno-logically privileged (e.g., thymus, uterus, brain, anterior eye chamber, and testicle). Because of their characteristics, seminal vesicles could be considered as immunologically privileged organs, but there is no worldwide experience that can confirm it. The purpose of the present study is to assess the viability and functionality of islet transplantation into seminal vesicles of diabetic rats. One hundred ninety inbred adult male syngeneic Lewis rats were used as donors (n = 72), receptors (n = 36), and controls(n = 11). Diabetes was chemically induced through a single intraperitoneal injection of streptozotocin. Groups of 1200 purified islets were introduced in the right seminal vesicle of diabetic rats. Diabetic control rats were sham transplanted. Body weight and glycemia were monitored every 2 d. Of transplanted rats, 16.7% achieved a good function due to islet engraftment, while 30.6% achieved a partially good response, and 52.7% were considered as nonresponding. This is the first report about islet transplantation into seminal vesicles of diabetic animals. Our results indicate that islet transplantation into rat seminal vesicles is technically possible, and that islets can function normally after engraftment into the wall of the seminal vesicle.
Luna
A
A
Department of Surgery in Hospital Universitari Germans Trias i Pujol Badalona, Barcelona, Spain.
Julián
J F
JF
Alba
A
A
Garcia-Cuyás
F
F
Broggi
M A
MA
Ciancio
G
G
Pujol-Borrell
R
R
Fernández-Llamazares
J
J
Vives-Pi
M
M
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D006085:Graft Survival; D006943:Hyperglycemia / Q000097:blood / Q000601:surgery; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D012669:Seminal Vesicles / Q000601:surgery*
D001786:Blood Glucose