Minute virus of mice small non-structural protein NS2 localizes within, but is not required for the formation of, Smn-associated autonomous parvovirus-associated replication bodies.
The non-structural proteins NS1 and NS2 of the parvovirus minute virus of mice (MVM) are required for efficient virus replication. It has previously been shown that NS1 and NS2 interact and colocalize with the survival motor neuron (Smn) gene product in novel nuclear structures that are formed late in infection, termed Smn-associated APAR (autonomous parvovirus-associated replication) bodies (SAABs). It is not clear what molecular viral intermediate(s) contribute to SAAB formation. The current results address the role of NS2 in SAAB formation. In highly synchronized wild-type MVM infection of murine A9(2L) cells, NS2 colocalizes with Smn and other SAAB constituents. An MVM mutant that does not produce NS2 still generates SAABS, albeit with a temporal delay. The lag in SAAB formation seen in the absence of NS2 is probably related to the temporal delay in virus replication, suggesting that, whilst NS2 is required for efficient viral infection, it is dispensable for SAAB formation.
Young
Philip J
PJ
Department of Veterinary Pathobiology, Life Sciences Center 471G, University of Missouri, Columbia, MO 65211-7310, USA.
Newman
Ann
A
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
Jensen
Klaus T
KT
Novozymes, 1AS.31, Novo Alle, DK-2880 Bagsværd, Denmark.
Burger
Lisa R
LR
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
Pintel
David J
DJ
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
Lorson
Christian L
CL
Department of Veterinary Pathobiology, Life Sciences Center 471G, University of Missouri, Columbia, MO 65211-7310, USA.
D000818:Animals; D001324:Autoantigens; D002460:Cell Line; D020541:Coiled Bodies / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D051379:Mice; D008915:Minute Virus of Mice / Q000472:pathogenicity / Q000502:physiology*; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication; D055517:snRNP Core Proteins
D001324:Autoantigens; D017411:Ribonucleoproteins, Small Nuclear; D017361:Viral Nonstructural Proteins; D055517:snRNP Core Proteins