Myocardial protective effect of octylguanidine against the damage induced by ischemia reperfusion in rat heart.
This study shows that the hydrophobic cation octylguanidine protects against myocardial damage induced by ischemia-reperfusion. The protective effect of the amine was analyzed after 5 min of coronary occlusion followed by 5 min reperfusion in rat hearts. ECG tracings from rats treated with an i.v., injection of 5 mg/kg of octylguanidine showed a total absence of post-reperfusion arrhythmias, conversely to what was observed in untreated rats. The histological images showed that myocardium fibers from treated rats were in good shape and retained their striae, also there was absence of edema. Furthermore, the accumulation of 201Tl in hearts from these rats indicated that the tissue did not suffer disruption or impairment in membrane functions. The above correlated with the fact that mitochondria isolated from the ventricular free wall from treated rats preserved their ability to synthesize ATP. We propose that the protective effect of octylguanidine might be due to its documented inhibitory action on the opening of mitochondrial non-specific pores, a mechanism which is associated in heart injury as induced by reperfusion.
Parra
Elías
E
Departamento de Bioquímica, Instituto Nacional de Cardiología, México.
Cruz
David
D
García
Gerardo
G
Zazueta
Cecilia
C
Correa
Francisco
F
García
Noemí
N
Chávez
Edmundo
E
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004562:Electrocardiography; D006146:Guanidines / Q000627:therapeutic use*; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology / Q000473:pathology; D020011:Protective Agents / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
D006146:Guanidines; D020011:Protective Agents; C015403:octylguanidine; D002118:Calcium