Synthesis of C2-chiral bifunctionalised spin labels and their application to troponin C.
An enantiomeric pair of C2-chiral bifunctionalised spin labels having a pyrrolidine nitroxide moiety, whose configurations were determined by X-ray crystal diffraction analysis, was prepared and applied to troponin C whose binding mode of double disulfide linkage was proved by EPR spectroscopy.
Chatani
Shunsuke
S
Graduate School of Human & Environmental Studies, Kyoto University, Sakyo-ku, Yoshida, Kyoto 606-8501, Japan.
Nakamura
Motoyoshi
M
Akahane
Hidenobu
H
Kohyama
Naoki
N
Taki
Masayasu
M
Arata
Toshiaki
T
Yamamoto
Yukio
Y
D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002645:Chickens; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D013113:Spin Labels; D013237:Stereoisomerism; D019209:Troponin C / Q000138:chemical synthesis / Q000737:chemistry*
D013113:Spin Labels; D019209:Troponin C; D002244:Carbon