Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles.
A branched peptide amphiphile system was designed for enhanced recognition of biotin on nanofibers formed by self-assembly of these molecules. Branching at a lysine residue was used to design peptide amphiphiles that are capable of presenting more than one epitope per molecule. We found that biotinylated branched structures form nanofibers that enhance recognition by the avidin protein receptor relative to similar nanostructures formed by linear peptide analogues. Biotin-avidin binding to the supramolecular nanofibers was characterized by measurement of fluorescence from nanofibers incubated with chromophore-conjugated avidin.
Guler
Mustafa O
MO
Department of Chemistry, Feinberg School of Medicine, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA.
Soukasene
Stephen
S
Hulvat
James F
JF
Stupp
Samuel I
SI
D001665:Binding Sites; D040761:Biomimetic Materials / Q000032:analysis / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D043942:Nanotubes / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation
D020099:Coated Materials, Biocompatible; D046912:Multiprotein Complexes; D010455:Peptides; D001710:Biotin