Inhibitory effects of Stephania tetrandra S. Moore on free radical-induced lysis of rat red blood cells.
Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.
Sekiya
Nobuyasu
N
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Hikiami
Hiroaki
H
Yokoyama
Koichi
K
Kouta
Kazufumi
K
Sakakibara
Iwao
I
Shimada
Yutaka
Y
Terasawa
Katsutoshi
K
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology; D001599:Berberine / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D031609:Stephania tetrandra / Q000737:chemistry*; D013997:Time Factors
D000470:Alkaloids; D000975:Antioxidants; D044182:Benzylisoquinolines; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D001599:Berberine; C060801:cyclanoline; C009438:tetrandrine; C060802:fangchinoline