title: Expression and antigenicity of virus-like particles of norovirus and their application for detection of noroviruses in stool samples.
abstract: Human noroviruses (NoVs), members of the genus Norovirus in the family Caliciviridae, are the leading agents of nonbacterial acute gastroenteritis worldwide. Human NoVs are currently divided into at least two genogroups, genogroup I (GI) and genogroup II (GII), each of which contains at least 14 and 17 genotypes. To explore the genetic and antigenic relationship among NoVs, we expressed the capsid protein of four genetically distinct NoVs, the GI/3 Kashiwa645 virus, the GII/3 Sanbu809 virus, the GII/5 Ichikawa754 virus, and the GII/7 Osaka10-25 virus in baculovirus expression system. An antigen enzyme-linked immunosorbent assay (ELISA) with hyperimmune serum against the four recombinant capsid proteins and characterized previously three capsid proteins derived from GI/1, GI/4, and GII/12 was developed to detect the NoVs antigen in stools. The antigen ELISA was highly specific to the homotypic strains, allowing assignment of a strain to a Norovirus genetic cluster within a genogroup.
authors.affiliation: Technical Marketing Department, Denka-Seiken Co., Ltd., Gosen, Niigata, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Kamata
authors.forename: Kuniko
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Mineyuki
authors.initials: M
authors.lastname: Okada
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakae
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Oseto
authors.forename: Katsuro
authors.initials: K
authors.lastname: Natori
authors.forename: Haruko
authors.initials: H
authors.lastname: Shirato-Horikoshi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Naokazu
authors.initials: N
authors.lastname: Takeda
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D017250:Caliciviridae Infections / Q000175:diagnosis; D036022:Capsid Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis; D006801:Humans; D029322:Norovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins