Vaccination against fowl cholera with acapsular Pasteurella multocida A:1.
We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
Chung
Jing Yeng
JY
Australian Research Council Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Vic. 3800, Australia.
Wilkie
Ian
I
Boyce
John D
JD
Adler
Ben
B
D000818:Animals; D016667:Bacterial Capsules / Q000502:physiology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D051379:Mice; D008807:Mice, Inbred BALB C; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
D001428:Bacterial Vaccines