Growth suppressing effect of garlic compound diallyl disulfide on prostate cancer cell line (PC-3) in vitro.
Prostate cancer is the most predominant cancer in men and prostate cancer related death increases every year. Till date, there is no effective therapy other than androgen ablation therapy. At this stage, induction of apoptosis is considered as a better strategy to control cancer. Previous studies reported that aged garlic extract suppresses cancer growth and enhances immune system against cancer. In the present study, diallyl disulfide, oil soluble organosulfur compound of garlic, was studied for its antiproliferative effect on prostate cancer cells in vitro. The suppression of cell growth was demonstrated by [(3)H]thymidine incorporation assay. Induction of DNA damage was assessed by agarose gel electrophoresis. The results showed that diallyl disulfide inhibited the growth of prostate cancer cells in a dose dependent manner, compared to the control. At 50 microM and 100 microM concentrations, diallyl disulfide induced DNA damage in PC-3 cells. It is concluded that diallyl disulfide, component of aged garlic extract, inhibits proliferation of prostate cancer cells through the induction of apoptosis.
Arunkumar
Arumugam
A
Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, India. j_arunakaran@hotmail.com
Vijayababu
Marati Radhakrishnan
MR
Kanagaraj
Palaniyandi
P
Balasubramanian
Karundevi
K
Aruldhas
Maria Michael
MM
Arunakaran
Jagadeesan
J
D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004220:Disulfides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; C028009:diallyl disulfide