title: N-acetyltransferase is involved in baicalein-induced N-acetylation of 2-aminofluorene and DNA-2-aminofluorene adduct formation in human leukemia HL-60 cells.
abstract: Many arylamine and hydrazine drugs are acetylated by cytosolic N-acetyltransferase (NAT). The human promyelocytic leukemia cell line (HL-60) has been shown to acetylate arylamine and contain NAT activity. The purpose of this study was to determine whether or not baicalein could affect N-acetylation of 2-aminofluorene (AF) in HL-60 cells. Acetylated and nonacetylated AF were determined by using high performance liquid chromatography. Baicalein displayed a dose-dependent inhibition of cytosolic and intact cells' NAT activity and reduced the number of viable cells. Time-course experiments showed that N-acetylation of AF, measured from intact HL-60 cells, was inhibited by baicalein for up to 48 h. Baicalein also decreased AF-DNA adduct formation in the examined cells. The effects of baicalein on NAT were examined by flow cytometry and NAT gene expression was examined by polymerase chain reaction. The results demonstrated that baicalein inhibited NAT1 mRNA gene expression and reduced the level of NAT in HL-60 cells. These results show that baicalein can affect the NAT activity of human leukemia cells in vitro.
authors.affiliation: Department of Medical Technology, Chungtai Institute of Health Sciences and Technology, Taichung 406, ROC.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Li
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Tyan
authors.forename: Yih-Min
authors.initials: YM
authors.lastname: Lee
authors.forename: Tang-Yi
authors.initials: TY
authors.lastname: Tsao
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Chuang
authors.forename: Hsiu-Maan
authors.initials: HM
authors.lastname: Kuo
authors.forename: Te-Chun
authors.initials: TC
authors.lastname: Hsia
authors.forename: Jen-Hung
authors.initials: JH
authors.lastname: Yang
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000107:Acetylation; D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000096:biosynthesis*; D015536:Down-Regulation; D044950:Flavanones / Q000494:pharmacology*; D005449:Fluorenes / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007527:Isoenzymes; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: C052668:2-aminofluorene-DNA complex; D018736:DNA Adducts; D044950:Flavanones; D005449:Fluorenes; D007527:Isoenzymes; D012333:RNA, Messenger; C012177:2-aminofluorene; C006680:baicalein; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1