title: Effects of serum deprivation on expression of proteolytic-related genes in chick myotube cultures.
abstract: We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24 h. Ubiquitin and proteasome subunits (C1 and C2) and calpains (m-, mu-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes.
authors.affiliation: Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science, Ikenodai, Tsukuba, Ibaraki, Japan. kaznaka@affrc.go.jp
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakashima
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
mesh_terms: D000818:Animals; D001483:Base Sequence; D002154:Calpain / Q000235:genetics; D002401:Cathepsin B / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D018076:DNA, Complementary; D006868:Hydrolysis; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D025801:Ubiquitin / Q000235:genetics
chemical_list: D016895:Culture Media, Serum-Free; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D025801:Ubiquitin; D002154:Calpain; D002401:Cathepsin B; D046988:Proteasome Endopeptidase Complex