title: [Clinical significance of heparanase and basic fibroblast growth factor expression in human non-small cell lung cancer].
abstract: OBJECTIVE
  To assess protein and mRNA expression levels of heparanase and basic fibroblast growth factor (bFGF) genes in human non-small cell lung cancer (NSCLC) and their roles in tumor invasion, metastasis and prognosis.
  
  
  METHODS
  A total of 115 paraffin-embedded and 45 fresh-frozen tissue specimens of NSCLC were studied by immunohistochemistry, Western Blot and in situ hybridization to evaluate the protein and mRNA expression status of heparanase and bFGF genes. The data was analyzed by SPSS statistical software.
  
  
  RESULTS
  Both human heparanase and bFGF were highly expressed in NSCLC cells, in contrast to none or a low expression in normal lung tissue. Expression of heparanase also showed a significantly higher than that in the normal tissue by Western blot (P = 0.041). Immunohistochemistry showed that heparanase expression was both cytoplasmic and membranous. The agreement between heparanase and bFGF was significant. A significant correlation was found between the expression of either protein and TNM stage, vascular invasion, lymphatic metastasis and microvascular density (MVD). Co-expression of the two proteins demonstrated an even higher correlation with the tumor stage and MVD. In addition, expression of bFGF correlated with tumor cell differentiation. Data of a multivariate analysis indicated that tumor cell differentiation, vascular invasion, lymphatic metastasis and expression of bFGF were identified as significant prognostic parameters.
  
  
  CONCLUSIONS
  Both heparanase and bFGF may play important roles in tumor angiogenesis, metastasis, and prognosis of NSCLC.
authors.affiliation: Department of Pathology, China Medical University, Shenyang 110001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Shu-yu
authors.initials: SY
authors.lastname: Li
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yu
authors.forename: Xue-shan
authors.initials: XS
authors.lastname: Qiu
authors.forename: Ping
authors.initials: P
authors.lastname: Hou
authors.forename: En-hua
authors.initials: EH
authors.lastname: Wang
authors.forename: Iris
authors.initials: I
authors.lastname: Pecker
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D011379:Prognosis; D015996:Survival Rate; D000099175:Heparanase
chemical_list: D016222:Fibroblast Growth Factor 2; D005966:Glucuronidase; D000099175:Heparanase