Amplification of 16S rRNA genes of Anaplasma species in China for phylogenetic analysis.
In this study, a phylogenetic tree was inferred through comparing five 16S rRNA gene sequences of four isolates of Anaplasma ovis and one of Anaplasma marginale in China with all nineteen 16S rRNA gene sequences deposited in GenBank (12 A. marginale, 3 A. ovis and 4 Anaplasma centrale derived from America, Uruguay, South Africa, Zimbabwe, Australia, Isreal and Japan). The analysis showed that all A. ovis isolated in China were separated into an A. ovis cluster, while the A. marginale in China was separated into an A. marginale cluster (see Fig. 1). This analysis demonstrated that there are at least two different Anaplasma species widespread among ruminants in North China.
Liu
Zhijie
Z
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 11, Lanzhou, Gansu 730 046, China.
Luo
Jianxun
J
Bai
Qi
Q
Ma
Miling
M
Guan
Guiquan
G
Guan
Guiguan
G
Yin
Hong
H
D000709:Anaplasma / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D042321:Anaplasma centrale / Q000145:classification / Q000235:genetics; D042322:Anaplasma marginale / Q000145:classification / Q000235:genetics; D042323:Anaplasma ovis / Q000145:classification / Q000235:genetics; D000712:Anaplasmosis / Q000175:diagnosis / Q000382:microbiology*; D000818:Animals; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005784:Gene Amplification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D012418:Ruminants / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S