title: Asthmatic bronchial epithelium activated by the proteolytic allergen Der p 1 increases selective dendritic cell recruitment.
abstract: BACKGROUND
  Airway dendritic cells (DCs) are crucial for allergen-induced sensitization and inflammation in allergic asthma. After allergen challenge, an increased number of DCs is observed in airway epithelium from patients with allergy.
  
  
  OBJECTIVE
  Because Der p 1, a cysteine protease allergen from Dermatophagoides pteronyssinus , induces chemokine production by bronchial epithelial cells (BECs), the purpose of this investigation was to evaluate the capacity of BEC exposed to Der p 1 to recruit DCs.
  
  
  METHODS
  Chemotactic activity of BEAS-2B, a bronchial epithelial cell line, and BECs from nonatopic controls and patients with allergic asthma was evaluated on the migration of precursors, immature and mature monocyte-derived DCs (MDDCs), and CD34 + -derived Langerhans cells (LCs).
  
  
  RESULTS
  C-C chemokine ligand (CCL)-2, CCL5, and C-X-C chemokine ligand 10 production by BEAS-2B and BEC was increased after Der p 1 exposure, whereas the proenzyme proDer p 1 devoid of enzymatic activity had no effect. Der p 1 stimulation of BEAS-2B and BEC from both groups increased significantly the recruitment of MDDC precursors, depending on CCL2, CCL5, and C-X-C chemokine ligand 10 production. In a reconstituted polarized epithelium, apical application of Der p 1 enhanced MDDC precursor migration into the epithelial layer. Moreover, Der p 1 stimulation of BEC from patients with asthma but not from controls increased the migration of LC precursors, mainly dependent on CCL20 secretion. No migration of immature and mature DCs was observed.
  
  
  CONCLUSIONS
  These data confirmed that BECs participate in the homeostasis of the DC network present within the bronchial epithelium through the secretion of chemokines. In allergic asthma, upregulation of CCL20 production induced LC recruitment, the role of which remains to be determined.
authors.affiliation: INSERM U416, IFR 17, Institut Pasteur, 1 rue du Professeur Calmette, BP 245, 59019 Lille Cedex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Pichavant
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Charbonnier
authors.forename: Solenne
authors.initials: S
authors.lastname: Taront
authors.forename: Anne
authors.initials: A
authors.lastname: Brichet
authors.forename: Benoît
authors.initials: B
authors.lastname: Wallaert
authors.forename: Joel
authors.initials: J
authors.lastname: Pestel
authors.forename: André-Bernard
authors.initials: AB
authors.lastname: Tonnel
authors.forename: Philippe
authors.initials: P
authors.lastname: Gosset
mesh_terms: D000328:Adult; D039741:Antigens, Dermatophagoides / Q000276:immunology / Q000494:pharmacology*; D060829:Arthropod Proteins; D001249:Asthma / Q000276:immunology*; D001980:Bronchi / Q000276:immunology*; D002460:Cell Line; D018925:Chemokines / Q000378:metabolism; D003546:Cysteine Endopeptidases; D005260:Female; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000276:immunology; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013234:Stem Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D018925:Chemokines; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1