[Analysis of trichothecenes in barley tea and beer by liquid chromatography/tandem mass spectrometry].
A simple method for analysis of trichothecenes [Type A: diacetoxyscirpenol, neosolaniol, HT-2 toxin, and T-2 toxin, Type B: deoxynivalenol, nivalenol, fusarenon-X, 3-acetyldeoxynivalenol, 15-acetyldeoxynivarenol] in barley tea and beer using liquid chromatography tandem mass spectrometry (LC/MS/MS) was developed. Trichothecenes were extracted with ethyl acetate-methanol (19:1). The solvent was evaporated to dryness and the residue was dissolved in water-methanol (3:1) for injection into the LC/MS/MS. The LC separation was performed with an octadecylated silica column at a flow-rate of 0.2 mL/min, using a mobile phase consisting of water, methanol and acetonitrile. MS/MS was used in multiple reaction monitoring, employing electrospray ionization (ESI-MRM). The recoveries of trichothecenes from drinks at 1 microg/L (Type A) and 10 microg/L (Type B) were 52.5-115.2% (barley tea) and 68.1-127.5% (beer). Five barley tea and ten beer samples were analyzed by this method. Trichothecenes were not detected in them. This method may have applications in quality assurance.
Suga
Keiko
K
Asahi Breweries, Ltd., Ibaraki, Japan.
Mochizuki
Naoki
N
Harayama
Koichi
K
Yamashita
Hiroshi
H
D001515:Beer / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry* / Q000382:microbiology; D013058:Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*
D014255:Trichothecenes