title: Methods for predicting superior genotypes under multiple environments based on QTL effects.
abstract: Methods were developed for predicting two kinds of superior genotypes (superior line and superior hybrid) based on quantative trait locus (QTL) effects including epistatic and QTL x environment interaction effects. Formulae were derived for predicting the total genetic effect of any individual with known QTLs genotype derived from the mapping population in a specific environment. Two algorithms, enumeration algorithm and stepwise tuning algorithm, were used to select the best multi-locus combination of all the putative QTLs. Grain weight per plant (GW) in rice was analyzed as a working example to demonstrate the proposed methods. Results showed that the predicted superior lines and superior hybrids had great superiorities over the F(1) hybrid, indicating large breeding potential remained for further improvement on GW. Results also showed that epistatic effects and their interaction with environments largely contributed to the superiorities of the predicted superior lines and superior hybrids. User-friendly software, QTLNetwork, version 1.0, was developed based on the methods in the present paper.
authors.affiliation: Institute of Bioinformatics, Zhejiang University, Hangzhou, Zhejiang, 310029, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000465:Algorithms; D002523:Edible Grain / Q000235:genetics; D004777:Environment; D004843:Epistasis, Genetic; D005838:Genotype; D006824:Hybridization, Genetic; D008957:Models, Genetic; D012275:Oryza / Q000235:genetics; D040641:Quantitative Trait Loci