title: Fibulin-5 interacts with fibrillin-1 molecules and microfibrils.
abstract: Fibulin-5 plays an important role in elastic fibre formation in vivo. We have investigated the molecular interactions between fibulin-5 and components of fibrillin-rich microfibrils which form a template for elastin. Fibulin-5 interacted in a dose-dependent manner with a fibrillin-1 N-terminal sequence and with tropoelastin, but not with MAGP-1 (microfibril-associated glycoprotein-1) or decorin. Fibulin-5 did not inhibit interactions between fibrillin-1 N- and C-terminal fragments, or fibrillin-1 interactions with tropoelastin. Fibulin-5 may provide a link between tropoelastin and microfibrils in the pericellular space during elastic fibre assembly.
authors.affiliation: Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Lyle J
authors.initials: LJ
authors.lastname: Freeman
authors.forename: Amanda
authors.initials: A
authors.lastname: Lomas
authors.forename: Nigel
authors.initials: N
authors.lastname: Hodson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sherratt
authors.forename: Kieran T
authors.initials: KT
authors.lastname: Mellody
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
authors.forename: Adrian
authors.initials: A
authors.lastname: Shuttleworth
authors.forename: Cay M
authors.initials: CM
authors.lastname: Kielty
mesh_terms: D003285:Contractile Proteins / Q000737:chemistry; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D020894:Microfibrils / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000737:chemistry; D000072260:RNA Splicing Factors; D054392:Adipokines
chemical_list: D003285:Contractile Proteins; D058575:Decorin; D016326:Extracellular Matrix Proteins; D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; D011509:Proteoglycans; D000072260:RNA Splicing Factors; C546878:DCN protein, human; C492373:FBLN5 protein, human; C000605722:FBN1 protein, human; D054392:Adipokines; C016598:microfibrillar protein