title: Enhanced sodium absorption in middle ear epithelial cells cultured at air-liquid interface.
abstract: CONCLUSIONS
  As we demonstrated previously that transcription of alpha-ENaC was correlated with oxygen tension in the culture medium, this study suggests that the increase in alpha-ENaC expression observed under ALI conditions may result from greater oxygenation of ME cells.
  
  
  OBJECTIVE
  The physiology of the middle ear (ME) is primarily concerned with keeping the cavities fluid-free, to allow transmission of sound vibrations from the eardrum to the inner ear. ME epithelial cells are thought to play a key role in this process as they actively absorb sodium and water in order to clear any excess fluid present in the cavities.
  
  
  METHODS
  As an air-liquid interface (ALI) model has been shown to improve differentiation and enhance sodium absorption in other respiratory epithelia, we established an ALI model for ME cells.
  
  
  RESULTS
  ME cells cultured under ALI conditions exhibited a fourfold increase in sodium absorption, which was not related to either a metabolic effect or to enhanced morphological differentiation, but instead to an increase in expression of the alpha-subunit of the epithelial sodium channel (alpha-ENaC).
authors.affiliation: Laboratoire d'Otologie Expérimentale, UMR 7060, Faculté Lariboisière-St-Louis, Assistance Publique des Hôpitaux de Paris, Université Paris VII, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Portier
authors.forename: R
authors.initials: R
authors.lastname: Kania
authors.forename: C
authors.initials: C
authors.lastname: Planès
authors.forename: W C
authors.initials: WC
authors.lastname: Hsu
authors.forename: S
authors.initials: S
authors.lastname: Couette
authors.forename: P
authors.initials: P
authors.lastname: Tran Ba Huy
authors.forename: P
authors.initials: P
authors.lastname: Herman
mesh_terms: D000042:Absorption / Q000502:physiology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000388:Air; D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D001826:Body Fluids / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D003584:Cytological Techniques; D004432:Ear, Middle / Q000166:cytology* / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005849:Gerbillinae; D042541:Intracellular Space / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism; D012964:Sodium / Q000493:pharmacokinetics*
chemical_list: D000255:Adenosine Triphosphate; D012964:Sodium