title: [Analysis of residual solvents in natural flavorings by headspace GC using the standard addition method].
abstract: Headspace GC using the standard addition method has been developed for the simultaneous determination of organic solvents in natural flavorings. The procedure can be outlined as follows: an aliquot of the sample is transferred to a 10 mL vial. To each vial, a DMSO solution containing solvents at different concentrations is added as the standard solution. The vials are kept at 50 degrees C (for automatic injection) or 40 degrees C (for hand-operated injection) for 40 minutes. One mL of the vapor phase in each vial is injected into a gas chromatograph equipped with an Aquatic-2 column (0.25 mm i.d. x 60 m). To evaluate this method, we conducted a performance study in collaboration with 10 laboratories, using ginger oleoresin. We analyzed 6 solvents (methanol, 2-propanol, acetone, dichloromethane, hexane, and 1,1,2-trichloroethene) for which the maximum residue limits are established in Japan's Specifications and Standards for Food Additives. Methanol and acetone existed in the ginger oleoresin, so only the other that four kinds of solvents were added to it. Eight of the laboratories used automatic injection, while the remaining two used hand-operated injection. Statistical analyses were conducted on the data obtained from the 8 laboratories. Repeatability standard deviations (RSDr) ranged from 4.3 to 11.4%, and reproducibility standard deviations (RSDR) ranged from 8.4 to 19.0%.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sato
authors.forename: Yoko
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Isagawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Tateba
authors.forename: Masahito
authors.initials: M
authors.lastname: Tomizawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Oosaki
authors.forename: Akio
authors.initials: A
authors.lastname: Hasebe
authors.forename: Saburo
authors.initials: S
authors.lastname: Shibuya
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nii
authors.forename: Ryuji
authors.initials: R
authors.lastname: Higashinaka
authors.forename: Ichiro
authors.initials: I
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tanamoto
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D019840:2-Propanol / Q000032:analysis; D000096:Acetone / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D005421:Flavoring Agents / Q000737:chemistry*; D020939:Zingiber officinale; D006586:Hexanes / Q000032:analysis; D000432:Methanol / Q000032:analysis; D008752:Methylene Chloride / Q000032:analysis; D012997:Solvents / Q000032:analysis*; D014240:Trichloroethanes / Q000032:analysis
chemical_list: D005421:Flavoring Agents; D006586:Hexanes; D012997:Solvents; D014240:Trichloroethanes; D000096:Acetone; C024567:1,1,2-trichloroethane; D008752:Methylene Chloride; D019840:2-Propanol; D000432:Methanol