The effect of Lactobacillus acidophilus and Bifidobacterium spp. administration on the morphology of the gastrointestinal tract, liver and pancreas in piglets.
The aim of the study was to investigate the influence of administration of probiotic bacteria on morphology of the gastrointestinal tract, liver and pancreas. The experiment was performed on 15 piglets at the age from 3 to 35 days, intragastrically administered with Bifidobacterium breve, B. animalis, and Lactobacillus acidophilus bacteria. In all piglets examined, the development of the gastrointestinal tract, liver and pancreas was observed to progress normally. After microflora administration to the piglets, an increase in the number of fibrocytes and fibroblasts was observed in the mucosa lamina propria of stomach and intestines. An increase was also reported in the number and activation of endocrine cells in the stomach and small intestines. The activity of alkaline and acid phosphatases, as well as succinic (SDH) and lactic (LDH) dehydrogenases, was found to be higher after the administration of probiotics. The administration of bacteria, especially of Lactobacillus acidophilus, caused an increase in the number of lymphocytes and lymphoid cells in lamina propria and intraepithelial lymphocytes in the small intestine. Enhanced proliferation of crypt cells was observed in the crypts of intestinal glands; however, there were no statistically significant differences in the PCNA index between the control and probiotic-administered groups. The performed study showed that the administration of probiotic bacteria has no negative impact on the morphology of the gastrointestinal tract, liver and pancreas and is found beneficial to its functioning and immune processes.
Babińska
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Department of Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland. Izabella.Babinska@uwm.edu.pl
Rotkiewicz
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T
Otrocka-Domagała
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I
D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development; D001644:Bifidobacterium / Q000502:physiology*; D004064:Digestive System / Q000254:growth & development*; D005260:Female; D041981:Gastrointestinal Tract / Q000254:growth & development; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology / Q000648:ultrastructure; D007779:Lactobacillus acidophilus / Q000502:physiology*; D008099:Liver / Q000254:growth & development; D008297:Male; D010179:Pancreas / Q000254:growth & development; D019936:Probiotics / Q000494:pharmacology*; D013552:Swine / Q000254:growth & development*