title: Advanced approaches for the characterization of a de novo designed antiparallel coiled coil peptide.
abstract: We report here an advanced approach for the characterization of the folding pattern of a de novo designed antiparallel coiled coil peptide by high-resolution methods. Incorporation of two fluorescence labels at the C- and N-terminus of the peptide chain as well as modification of two hydrophobic core positions by Phe/[15N,13C]Leu enable the study of the folding characteristics and of distinct amino acid side chain interactions by fluorescence resonance energy transfer (FRET) and NMR spectroscopy. Results of both experiments reveal the antiparallel alignment of the helices and thus prove the design concept. This finding is also supported by molecular dynamics simulations. Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) in combination with NMR experiments was used for verification of the oligomerization equilibria of the coiled coil peptide.
authors.affiliation: Free University Berlin, Department of Chemistry-Organic Chemistry, Takustrasse 3, 14195, Berlin, Germany.
authors.forename: Kevin
authors.initials: K
authors.lastname: Pagel
authors.forename: Karsten
authors.initials: K
authors.lastname: Seeger
authors.forename: Bettina
authors.initials: B
authors.lastname: Seiwert
authors.forename: Alessandra
authors.initials: A
authors.lastname: Villa
authors.forename: Alan E
authors.initials: AE
authors.lastname: Mark
authors.forename: Stefan
authors.initials: S
authors.lastname: Berger
authors.forename: Beate
authors.initials: B
authors.lastname: Koksch
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D031541:Fluorescence Resonance Energy Transfer; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010455:Peptides