Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citratus (DC.) Stapf.).
Methanol, MeOH/water extracts, infusion, and decoction of Cymbopogon citratus were assessed for free radical scavenging effects measured by the bleaching of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, scavenging of the superoxide anion, and inhibition of the enzyme xanthine oxidase (XO) and lipid peroxidation in human erythrocytes. The extracts presented effect in the DPPH and superoxide anion assay, with values ranging between 40 and 68% and 15-32% at 33 and 50 microg/mL, respectively, inhibited lipid peroxidation in erythrocytes by 19-71% at 500 microg/mL and were inactive toward the XO at 50 microg/mL. Isoorientin, isoscoparin, swertiajaponin, isoorientin 2' '-O-rhamnoside, orientin, chlorogenic acid, and caffeic acid were isolated and identified by spectroscopic methods. Isoorientin and orientin presented similar activities toward the DPPH (IC(50): 9-10 microM) and inhibited lipid peroxidation by 70% at 100 microg/mL. Caffeic and chlorogenic acid were active superoxide anion scavengers with IC(50) values of 68.8 and 54.2 microM, respectively, and a strong effect toward DPPH. Caffeic acid inhibited lipid peroxidation by 85% at 100 microg/mL.
Cheel
José
J
Instituto de Química de Recursos Naturales and Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, Casilla 747, Talca, Chile.
Theoduloz
Cristina
C
Rodríguez
Jaime
J
Schmeda-Hirschmann
Guillermo
G
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001713:Biphenyl Compounds; D031743:Cymbopogon / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D000432:Methanol; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D000975:Antioxidants; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D005419:Flavonoids; D016166:Free Radical Scavengers; D008055:Lipids; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase; D000432:Methanol