[Studies on the anticancer effects of total alkaloid from Viscum coloratum].
OBJECTIVE
To study the anticancer effects of total alkaloid from Viscum coloratum in vivo and vitro.


METHODS
In vitro, MTT assays were used t o measure the inhibitory effect. Cells at period of logarithmic growth were incubated for 24 hours. Then total alkaloid of various concentrations were added. 24 hours later, supernatant was removed and MTT was added. 4 hours after that, DMSO was added, then 30 minutes later, A value was measured. In vivo, suspension of carcinoma cells was implanted in the mice's limbs subcutaneously, 0.2mL each. 24 hours later, the mice were grouped randomly. Fed by total alkaloid continuously for 7 days, the mice were sacrificed. The tumors were weighed and calculated the inhibitory rates.


RESULTS
In vitro, it shows that total alkaloid has prominent inhibitory effect on the growth of carcinoma cells. In vivo, it shows that total alkaloid can inhibit the growth of tumors and prolong the survival days of the mice bearing tumors.


CONCLUSIONS
Total alkaloid from Viscum coloratum has the activities of anticancer.
Peng
Hai-yan
HY
The First Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Zhang
Yong-hong
YH
Han
Ying
Y
Wang
Meng
M
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000473:pathology; D005260:Female; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D028181:Viscum / Q000737:chemistry
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic