title: [Total radical trapping antioxidants parameters on plasma in smoking and non-smoking matched maternal-cord pairs].
abstract: Oxidative stress in smoking pregnant women is assumed to be enhanced by oxidants and free radicals of tobacco smoke. Therefore we studied the effect of cigarette smoking during pregnancy on plasma total radical trapping antioxidants parameters (TRAP) in mothers and their babies. Pregnant women were selected into the groups according to concentration of cotinine in serum. TRAP was determined by method of Wayner et al. using oxygen electrode (YSI Model 5331). The plasma concentration of TRAP was lower in smoking pregnant women than in tobacco abstinent group (533.0 micromol/l vs 600.0 micromol/l in I trimester, 578.0 micromol/l vs 583.5 micromol/l in II trimester and 583.5 micromol/l vs 589.0 micromol/l in III trimester. The differences were statistically significant in first and second trimester (p<0.05). Value of TRAP in umbilical cord blood from newborns of smoking mothers was significantly lower and amounted only 74% of that observed in non-smoking ones (366.5 micromol/l vs 492.0 micromol/l; p<0.001). Plasma level of TRAP was significantly correlated with serum concentration of cotinine both in smoking pregnant women (r=-0.32; p<0.01) and in umbilical cord blood of their children (r=-0.49; p<0.01). Our results indicate that smoking depletes plasma TRAP in mothers and their babies. Decreased level of total plasma antioxidants parameters may have a negative effect on antioxidant protection systems in neonates.
authors.affiliation: Zakład Biochemii i Diagnostyki Laboratoryjnej Instytutu Matki i Dziecka w Warszawie. lipidy@imid.med.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chełchowska
authors.forename: Teresa
authors.initials: T
authors.lastname: Laskowska-Klita
authors.forename: Jerzy
authors.initials: J
authors.lastname: Leibschang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D003367:Cotinine / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D018384:Oxidative Stress; D011247:Pregnancy; D012907:Smoking / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003367:Cotinine