Nested genes: biological implications and use of AFM for analysis.
A "nested" gene is located within the boundaries of a larger gene, often within an intron and in the opposite orientation. Such structures are common in bacteria and viruses, but have also been described in higher species as diverse as Drosophila and humans. Expression of nested and host genes may be simultaneously up-regulated due to use of common enhancers, or down-regulated through steric hindrance or interference caused by annealing of the complementary RNAs, leading to degradation. Methods for RNA analysis such as RT-PCR and in situ hybridization reveal the presence of specific mRNAs, but do not address regulation of expression within a single cell at a single genetic locus. Atomic force microscopy is a relatively new technology, which allows visualization of the movement of an RNA polymerase along a DNA template. The potential of this technology includes a greater molecular understanding of cellular decision making processes, leading to enhanced opportunities to intervene in disease progression through use of novel treatment modalities.
Gibson
Carolyn W
CW
University of Pennsylvania School of Dental Medicine, Department of Anatomy and Cell Biology, 240 S. 40th Street, Philadelphia, PA 19104, USA. gibson@biochem.dental.upenn.edu
Thomson
Neil H
NH
Abrams
William R
WR
Kirkham
Jennifer
J
D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D032041:Nested Genes / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D004247:DNA; D012321:DNA-Directed RNA Polymerases