[Effect of conjugated linoleic acid on gene expression of adiponectin of obese rat fed with high fat diet].
OBJECTIVE
To study the effect of conjugated linoleic acid (CLA) on expression of adiponectin in white adipose tissue of obese rats.


METHODS
Male Wistar rats were randomly divided into control group, high-fat group and high fat + CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight), we observed the effect of CLA on serum insulin and glucose levels of obese rats, and the reverse transcription polymerase chain reaction (RT-PCR) technique was used to measure the expression level of adiponectin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA.


RESULTS
The serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) microIU/ml, (5.09 +/- 0.66) mmol/L. The supplement of CLA decreased the hyperinsulinemia and hyperglycemia, the serum insulin in CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight) were (6.99 +/- 1.77) microIU/ml, (7.36 +/- 1.48) microIU/ml, (7.85 +/- 1.60) microIU/ml (P < 0.05), and glucose were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L (P < 0.05), (4.06 +/- 0.63) mmol/L (P < 0.05), CLA can increase the expression of adiponectin and PPARgamma in adipose tissue of obese rat.


CONCLUSIONS
The CLA might improve the insulin resistance of the obese rat and increase the expression of adiponectin mRNA, which might possibly act through activating PPARgamma.
Zhou
Xiao-rong
XR
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150001, China.
Sun
Chang-hao
CH
Wang
Hai-ying
HY
Jiang
Li-ying
LY
Liu
Rong
R
D052242:Adiponectin / Q000096:biosynthesis* / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D007333:Insulin Resistance / Q000502:physiology; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D052242:Adiponectin; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D012333:RNA, Messenger