title: An ex vivo model for reperfusion of laryngotracheal grafts.
abstract: OBJECTIVE
  To describe the development of an ex vivo model to facilitate the study of ischemia-reperfusion injury in laryngotracheal grafts taken from Minnesota minipigs.
  
  
  METHODS
  This is a descriptive study.
  
  
  METHODS
  Laryngotracheal grafts from Minnesota minipigs were harvested and placed in cold storage for 3 hours. Autologous blood was used to reperfuse the graft for up to 8 hours using the described reperfusion model.
  
  
  RESULTS
  By altering retrieval technique and pressures within the graft, we demonstrated healthy grafts after 8 hours of reperfusion.
  
  
  CONCLUSIONS
  Ex vivo reperfusion offers a range of experimental advantages over in vivo reperfusion including close control of interventions, ease of outcome measurement, and reduction in animal use. This model is now ready to be used for further studies of interventions to reduce ischemia-reperfusion injury in these grafts.
authors.affiliation: Division of Surgery, Department of Clinical Medicine, University of Bristol, Bristol, UK. emmabarker@doctors.org.uk
authors.forename: Emma
authors.initials: E
authors.lastname: Barker
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
authors.forename: Pamela
authors.initials: P
authors.lastname: Murison
authors.forename: Alan
authors.initials: A
authors.lastname: Jones
authors.forename: Karin
authors.initials: K
authors.lastname: Haverson
authors.forename: Mick
authors.initials: M
authors.lastname: Bailey
authors.forename: Martin
authors.initials: M
authors.lastname: Birchall
mesh_terms: D000818:Animals; D001769:Blood; D007260:Infusion Pumps; D007830:Larynx / Q000637:transplantation*; D017078:Laser-Doppler Flowmetry; D023421:Models, Animal; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000627:therapeutic use; D009929:Organ Size; D010106:Oxygenators; D012034:Refrigeration; D015424:Reperfusion / Q000379:methods; D015427:Reperfusion Injury / Q000517:prevention & control; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D020858:Tissue and Organ Harvesting; D014132:Trachea / Q000637:transplantation*
chemical_list: D019188:Organ Preservation Solutions