Generation and destruction of unstable reagent in flow injection system: determination of acetylcysteine in pharmaceutical formulations using bromine as reagent.
A flow injection spectrophotometric procedure was developed for determination of acetylcysteine in sachets and liquid formulations. The determination of this drug was carried out by reacting it with bromine chemically generated in flow injection system monitored continuously at 400 nm. Acetylcysteine reacts with bromine causing a decrease in the absorbance that is proportional to the analyte concentration. The bromine in excess was destroyed on-line by an ascorbic acid solution before the discard. The calibration curve for acetylcysteine determination was linear in the concentration range from 1.6 x 10(-4) to 1.6 x 10(-3) mol/l with a detection limit of 8.0 x 10(-5) mol/l. The relative standard deviation (R.S.D.) was lesser than 1.2% for a solution containing 5.3 x 10(-4) mol/l acetylcysteine (n=10), and 60 determinations per hour were obtained.
Suarez
Willian Toito
WT
Centro de Ciências Exatas e de Tecnologia, Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, CEP 13.560-970 São Carlos, São Paulo, Brazil.
Vieira
Heberth Juliano
HJ
Fatibello-Filho
Orlando
O
D000111:Acetylcysteine / Q000032:analysis*; D001205:Ascorbic Acid / Q000737:chemistry; D001966:Bromine / Q000737:chemistry*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D003683:Dehydroascorbic Acid / Q000737:chemistry; D017022:Flow Injection Analysis; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D019999:Pharmaceutical Solutions; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D001205:Ascorbic Acid; D001966:Bromine; D000111:Acetylcysteine; D003683:Dehydroascorbic Acid