Detection and analysis of alternative splicing in the silkworm by aligning expressed sequence tags with the genomic sequence.
We identified 277 alternative splice forms in silkworm genes based on aligning expressed sequence tags with genomic sequences, using a transcript assembly program. A large fraction (74%) of these alternative splices are located in protein-coding regions and alter protein products, whereas only 26% are in untranslated regions. From the alternative splices located in protein-coding regions, some (43%) affect protein domains that bind various biological molecules. The vast majority of the detected alternative forms in this study appear to be novel, and potentially affect biologically meaningful control of function in silkworm genes. Our results indicate that alternative splicing in silkworm largely produces protein diversity and functional diversity, and is a widely used mechanism for regulating gene expression.
Zha
X-F
XF
The Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing, China.
Xia
Q-Y
QY
Zhao
P
P
Li
J
J
Duan
J
J
Wang
Z-L
ZL
Qian
J-F
JF
Xiang
Z-H
ZH
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D019295:Computational Biology; D017931:DNA Primers; D020224:Expressed Sequence Tags; D016678:Genome; D016366:Open Reading Frames / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers