Influence of seed extract of Syzygium Cumini (Jamun) on mice exposed to different doses of gamma-radiation.
The radioprotective activity of the hydroalcoholic extract of jamun seeds (SCE) was studied in mice exposed to different doses of gamma radiation. The mice were injected with 0, 5, 10, 20, 40, 60, 80, 100, 120, 140 or 160 mg/kg body weight of SCE, before exposure to 10 Gy of gamma radiation, to select the optimum dose of radiation protection. The 80 mg/kg SCE was found to offer highest protection, therefore, further studies were carried out using this dose. The drug was more effective when administered through the intraperitoneal route at equimolar doses than the oral route. Since higher survival was observed for the i.p. route (50%), than the oral route (29.2%), all other studies were carried out by injecting SCE intraperitoneally. The mice treated with 80 mg/kg body weight SCE intraperitoneally before exposure to 6, 7, 8, 9, 10 and 11 Gy of gamma radiation showed reduction in the symptoms of radiation sickness and mortality at all exposure doses and caused a significant increase in the animal survival when compared with the concurrent double distilled water (DDW) + irradiation group. The SCE treatment protected mice against the gastrointestinal as well as bone marrow deaths and the DRF was found to be 1.24.
Jagetia
Ganesh Chandra
GC
Department of Radiobiology, Kasturba Medical College, Manipal (India). gc.jagetia@kmc.manipal.edu
Baliga
Manjeshwar Shrinath
MS
Venkatesh
Ponemone
P
D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D007274:Injections, Intraperitoneal; D008136:Longevity / Q000528:radiation effects; D008297:Male; D051379:Mice; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage*; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000209:etiology* / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry*; D016019:Survival Analysis; D027842:Syzygium / Q000737:chemistry*
D010936:Plant Extracts; D011837:Radiation-Protective Agents