title: Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids.
abstract: An investigation of the liquid chromatography of the minor capsaicinoids in a commercial capsaicinoid mixture is reported. Twelve stationary phases including C8, C18, C30, phenyl, and cation-exchange chemistries were examined in combination with isocratic aqueous methanol and aqueous acetonitrile mobile phases. A phenyl stationary phase and aqueous acetonitrile mobile phase baseline-resolved 7 of 11 capsaicinoids, and selected ion chromatograms (LC-ESI-MS) demonstrated this was the most effective reversed-phase separation. Argentation chromatography with an alkyl or phenyl column and aqueous silver nitrate-methanol mobile phase revealed the presence of the 6-ene-8-methyl and 6-ene-9-methyl homocapsaicin isomers and the absence of 7-ene-9-methyl homocapsaicin. A mixed phenyl-cation-exchange stationary phase (charged with silver ion) enabled unique and useful separations of the capsaicinoids.
authors.affiliation: Department of Chemistry, Oberlin College, 119 Woodland Street, Oberlin, OH 44074, USA.
authors.forename: Robert Q
authors.initials: RQ
authors.lastname: Thompson
authors.forename: Karen W
authors.initials: KW
authors.lastname: Phinney
authors.forename: Lane C
authors.initials: LC
authors.lastname: Sander
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Welch
mesh_terms: D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D015394:Molecular Structure; D012834:Silver
chemical_list: D012834:Silver; D002211:Capsaicin