[Neuroprotection by resveratrol against cytotoxicity of oxidized low density lipoprotein in PC12 cells].
OBJECTIVE
To investigate neuroprotection of resveratrol against cytotoxicity of oxidized low density lipoprotein (oxLDL) in PC12 cells.


METHODS
PC12 cells were pretreated with resveratrol for one hour as a neuron model and then exposed to oxLDL at varied concentrations. Effects of resveratrol on cell viability, permeability of cell membrane, cell nucleus and activity of caspase-3 were evaluated with MTT assay, lactic acid dehydrogenase (LDH) release assay, DNA fragmentation (TUNEL) assay and assay for caspase-3 activity (caspase assay).


RESULTS
Cell viability, LDH release rate, percentage of cells with positive TUNEL and activity of caspase-3 were (62 +/- 3)%, (23 +/- 3)%, (26 +/- 5)% and (0.811 +/- 0.049) mol.min(-1).microg(-1), vs (84 +/- 7)%, (13 +/- 4)%, (12 +/- 4)% and (0.553 +/- 0.048) mol.min(-1).microg(-1) in PC12 cells treated with 10 mg/L oxLDL vs in those treated with 10 mg/L oxLDL plus 50 micromol/L resveratrol, respectively, with statistically significant difference.


CONCLUSIONS
Resveratrol could attenuate cytotoxicity induced by oxLDL in PC12 cells with neuroprotection.
Cao
Li
L
Department of Neurology, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Wang
Yong-jun
YJ
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000633:toxicity*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D008077:Lipoproteins, LDL; D018696:Neuroprotective Agents; D013267:Stilbenes; C099252:oxidized low density lipoprotein; D000077185:Resveratrol