title: Thyrotropin-releasing hormone induced thermogenesis in Syrian hamsters: site of action and receptor subtype.
abstract: Early work in our laboratory has revealed the important role played by thyrotropin-releasing hormone (TRH) in the arousal from hibernation in Syrian hamsters. In the present study, we investigated the thermogenic mechanism of TRH in Syrian hamsters. Six to 10 female Syrian hamsters were used in the respective experiments. Intracerebroventricular (icv) injection of TRH elevated the intrascapular brown adipose tissue (IBAT) temperature (T(IBAT)) and rectal temperature (T rec) in Syrian hamsters. Thermogenic response of icv TRH was suppressed by bilateral denervation of the sympathetic nerve. Icv injection of TRH increased the norepinephrin (NE) turnover rate in IBAT without affecting the total serum triiodothyronine (T3) level. Moreover, TRH microinjections into the dorsomedial hypothalamus (DMH), preoptic area (PO), anterior hypothalamus (AH) and ventromedial hypothalamus (VMH) induced T(IBAT) and T(rec) increases. However, neither T(IBAT) nor T rec was affected by similar TRH administrations into the lateral hypothalamus and posterior hypothalamus. Interestingly, although TRH-induced hyperthermia was suppressed by pretreatment of anti-TRH-R1 antibodies, no changes were induced by anti-TRH-R2 antibodies. These results suggest that the sites of action of TRH associated with thermogenesis are probably localized in the DMH, PO, AH and VMH. In addition, TRH-induced thermogenesis is probably elicited by facilitation of the sympathetic nerve system via the central TRH-R1 irrelevant of T3.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Science Fukuyama University, 1, Gakuen-cho, Fukuyama, Hiroshima, 729-0292, Japan.
authors.forename: Mitsuteru
authors.initials: M
authors.lastname: Shintani
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
authors.forename: Mayuko
authors.initials: M
authors.lastname: Monden
authors.forename: Hirohito
authors.initials: H
authors.lastname: Shiomi
mesh_terms: D002001:Adipose Tissue, Brown / Q000294:innervation / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D006224:Cricetinae; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D007276:Injections, Intraventricular; D007367:Intercostal Nerves / Q000601:surgery; D008647:Mesocricetus; D009638:Norepinephrine / Q000378:metabolism*; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000378:metabolism*; D013562:Sympathectomy; D022722:Thermogenesis / Q000502:physiology*; D013973:Thyrotropin-Releasing Hormone / Q000008:administration & dosage / Q000502:physiology*
chemical_list: D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone; D009638:Norepinephrine