title: HPV16, HPV18, and HIV infection may influence cervical cytokine intralesional levels.
abstract: Infection with oncogenic human papillomavirus (HPV) is considered to be the major risk to cervical cancer. This study analyzed the influence of HPV infection on cytokine intralesional levels in cervical lesion in the presence or not of HIV infection. Cervical biopsies from 42 women were studied. HPV detection and typing were performed using amplified DNA hybridized with sequence-specific primers, and cytokine intralesional levels were detected using ELISA. HPV16+ biopsies exhibited increased IFN-gamma and IL-10 when compared to HPV16- (P = 0.03 and 0.04, respectively). HPV18+ biopsies exhibited decreased TNF-alpha (P = 0.009) and IFN- gamma (P = 0.01) when compared to HPV18-. In accordance to HIV status, HIV-/HPV16+ patients exhibited increased IFN-gamma when compared to those presenting HIV-/HPV16- (P = 0.007). HIV-/HPV18+ patients presented decreased IFN-gamma when compared to HIV-/HPV18- (P = 0.02). These results suggest that the presence of HPV16 infection may influence cervical lesion installation, and irrespective of HIV status, HPV18 infection may be more aggressive than HPV-16.
authors.affiliation: Department of General and Specialized Nursing, School of Nursing of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-902 Ribeirão Preto, SP, Brazil.
authors.forename: Ana Paula M
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Maria Alice G
authors.initials: MA
authors.lastname: Gonçalves
authors.forename: Geraldo
authors.initials: G
authors.lastname: Duarte
authors.forename: Fernando Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: Renata T
authors.initials: RT
authors.lastname: Simões
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Donadi
mesh_terms: D000293:Adolescent; D000328:Adult; D002584:Cervix Uteri / Q000378:metabolism* / Q000821:virology; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000503:physiopathology / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000821:virology
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma