Selection of D2S3, an Aedes aegypti (Diptera: Culicidae) strain with high oral susceptibility to Dengue 2 virus and D2MEB, a strain with a midgut barrier to Dengue 2 escape.
Family-based phenotypic selection was used to breed two genetic strains of Aedes aegypti L. that differ in susceptibility to infection with dengue serotype 2 virus (DEN-2) strain JAM1409. A Dengue 2 Susceptible on 3 chromosomes (D2S3) strain was bred from Ae. aegypti aegypti and Ae. aegyptiformosus P1 parents to have a high midgut infection rate (MIR) and a high disseminated infection rate (DIR). A Dengue 2 Midgut Escape Barrier (D2MEB) strain was bred from D2S3 and Houston P1 parents to have a high MIR and a low DIR. After selection in the F2 generation, single strand conformation polymorphism genotypes were determined at cDNA marker loci throughout the genome to test for Mendelian ratios and thereby identify regions containing deleterious or lethal alleles. Both strains were orally challenged with two other DEN-2 genotypes, two DEN-1 genotypes, one DEN-3 genotype, and two DEN-4 genotypes. There were significant differences in MIR and DIR for the different virus strains in both the D2S3 and the D2MEB mosquito lines.
Bennett
Kristine E
KE
Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft. Collins, CO 80523, USA.
Beaty
Barry J
BJ
Black
William C
WC
D000330:Aedes / Q000235:genetics* / Q000821:virology*; D000818:Animals; D001947:Breeding; D003716:Dengue Virus / Q000145:classification / Q000235:genetics; D005260:Female; D005838:Genotype; D008297:Male; D009055:Mouth; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012641:Selection, Genetic; D012703:Serotyping