title: Scoparone from Artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by interferon-gamma Plus LPS.
abstract: Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-gamma/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-gamma/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.
authors.affiliation: Department of Skin & Beauty, Seojeong College, Yangju 482-860, Korea.
authors.forename: Seon Il
authors.initials: SI
authors.lastname: Jang
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Woo-Yiel
authors.initials: WY
authors.lastname: Lee
authors.forename: Kyung Chell
authors.initials: KC
authors.lastname: Kwak
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Baek
authors.forename: Gyu Beum
authors.initials: GB
authors.lastname: Kwak
authors.forename: Young-Gab
authors.initials: YG
authors.lastname: Yun
authors.forename: Tae-Oh
authors.initials: TO
authors.lastname: Kwon
authors.forename: Hun Taeg
authors.initials: HT
authors.lastname: Chung
authors.forename: Kyu-Yun
authors.initials: KY
authors.lastname: Chai
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2; D016207:Cytokines / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013268:Stimulation, Chemical; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C022616:thiazolyl blue; C018145:scoparone; D015232:Dinoprostone