[Changes in the expression of vital cytokines for pancreatic development in early embryo].
OBJECTIVE
To clarify the changes in the expression of vital cytokines for pancreatic development in early embryo and hence provide preclinical data regarding embryonic pancreas transplanted for treatment, of diabetes mellitus.


METHODS
Sample collection was conducted in accordance to the principle of informed consent. Histochemical S-ABC method was adopted in studying pancreas at 7-12 weeks of gestation, and analysis was made on vital cytokines expression as well as the differentiation and forming of pancreatic islets.


RESULTS
It was found that the expression of Insulin, Glucagon, Somatostatin and Cytokeratin begins at 7 weeks, and the change is consistent with the differentiation and formation of pancreatic islets. IGF-I and F-VIII appear at 12 weeks.


CONCLUSIONS
Insulin, Glucagon, Somatostatin, Cytokeratin, IGF-I and F-VIII play a regulatory role in the development of embryonic pancreas and are related to the differentiation and formation of endocrine and exocrine glands of pancreas. The pancreas after 12 weeks of gestation can be used as a donated graft for pancreas transplantation.
Yang
Kai-ming
KM
Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Ai-dong
AD
Yang
Hui-jun
HJ
Wang
Ting-hua
TH
Mei
Yan
Y
Zhou
Hong-ying
HY
Jiang
Ji-ying
JY
D005260:Female; D005865:Gestational Age; D005934:Glucagon / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D010179:Pancreas / Q000196:embryology*; D011247:Pregnancy; D013004:Somatostatin / Q000096:biosynthesis* / Q000235:genetics
D013004:Somatostatin; D007334:Insulin-Like Growth Factor I; D005934:Glucagon