title: Casein haplotype structure in five Italian goat breeds.
abstract: The aim of this work was to investigate the genetic structure of the casein gene cluster in 5 Italian goat breeds and to evaluate the haplotype variability within and among populations. A total of 430 goats from Vallesana, Roccaverano, Jonica, Garganica, and Maltese breeds were genotyped at alphas1-casein (CSN1S1), alphas2-casein, (CSN1S2), beta-casein (CSN2), and kappa-casein (CSN3) loci using several genomic techniques and milk protein analysis. Casein haplotype frequencies were estimated for each breed. Principal component analysis was carried out to highlight the relationship among breeds. Allele and haplotype distributions indicated considerable differences among breeds. The haplotype CSN1S1*F- CSN1S2*F-CSN3*D occurred in all breeds with frequencies >0.100 and was the most common haplotype in the Southern breeds. A high frequency of CSN1S1*0-CSN1S2*C-CSN3*A haplotype was found in Vallesana population (0.162). Principal component analysis clearly separated the Northern and Southern breeds by the first component. The variability of the caprine casein loci and variety of resulting haplotypes should be exploited in the future using specific breeding programs aiming to preserve biodiversity and to select goat genetic lines for specific protein production.
authors.affiliation: Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, via Leonardo da Vinci 44, 10095 Grugliasco, Torino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Sacchi
authors.forename: S
authors.initials: S
authors.lastname: Chessa
authors.forename: E
authors.initials: E
authors.lastname: Budelli
authors.forename: P
authors.initials: P
authors.lastname: Bolla
authors.forename: G
authors.initials: G
authors.lastname: Ceriotti
authors.forename: D
authors.initials: D
authors.lastname: Soglia
authors.forename: R
authors.initials: R
authors.lastname: Rasero
authors.forename: E
authors.initials: E
authors.lastname: Cauvin
authors.forename: A
authors.initials: A
authors.lastname: Caroli
mesh_terms: D000818:Animals; D001947:Breeding; D002364:Caseins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D006041:Goats / Q000235:genetics*; D006239:Haplotypes; D007558:Italy; D008297:Male; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000662:veterinary; D025341:Principal Component Analysis
chemical_list: D002364:Caseins