The first two-dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins.
Cytosolic proteins of Schizosaccharomyces pombe were separated by two-dimensional (2-D) gel electrophoresis, to construct the first 2-D reference map. In the pI range 4-7, more than 500 spots were detected by silver staining, and 70 different proteins corresponding to 111 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and tandem mass spectrometry, where necessary. In the pI range 6-9, approximately 330 spots were detected, and 31 proteins corresponding to 38 spots were identified by mass spectrometry. More than 50% of the identified proteins were involved in amino acid, carbohydrate or nucleotide metabolism, and energy production. A second large group of identified proteins comprises heat shock and other stress related proteins and chaperones.
Sun
Namkyu
N
Laboratory of Protein Chemistry, Chungnam National University, Daejeon, South Korea.
Jang
Jaewon
J
Lee
Seunghee
S
Kim
Sunkyung
S
Lee
Seunghyun
S
Hoe
Kwang-Lae
KL
Chung
Kyung-Sook
KS
Kim
Dong-Uk
DU
Yoo
Hyang-Sook
HS
Won
Misun
M
Song
Kyung Bin
KB
D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome; D029702:Schizosaccharomyces pombe Proteins