title: Treatment of streptozotocin-induced diabetes mellitus in rats by transplantation of islet cells from two major histocompatibility complex disparate rats in combination with intra bone marrow injection of allogeneic bone marrow cells.
abstract: BACKGROUND
  We have established a new method for the transplantation of allogeneic pancreatic islets obtained from two different rat strains in combination with a newly developed bone marrow transplantation (BMT) method in which bone marrow cells (BMCs) are directly injected into the bone marrow cavity (intra bone marrow BMT [IBM-BMT]).
  
  
  METHODS
  Streptozotocin-induced diabetic Brown Norway (BN: RT1A(n)) rats were injected with fludarabine, irradiated with 5.0 Gy x 2, and BMCs from two allogeneic rat strains, Fischer 344 (F344: RT1A(1)) and PVG (PVG: RT1A(c)), were then directly injected into the bone marrow cavity (IBM-BMT). Simultaneously, approximately 600 pancreatic islets (PIs) from F344 and PVG rats were mixed and transplanted into the liver by way of the portal vein.
  
  
  RESULTS
  All the recipients thus treated showed normoglycemia 30 days after the treatment. Hematolymphoid cells were completely reconstituted with the two donor-type cells, and immunologic tolerance to F344 and PVG major histocompatibility complex (MHC) determinants were induced.
  
  
  CONCLUSIONS
  The transplantation of PIs from two MHC-disparate donors was completely achieved in combination with IBM-BMT, resulting in the improvement of blood glucose levels and the amelioration of diabetes mellitus.
authors.affiliation: First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Taira
authors.forename: Muneo
authors.initials: M
authors.lastname: Inaba
authors.forename: Keizo
authors.initials: K
authors.lastname: Takada
authors.forename: Susumu
authors.initials: S
authors.lastname: Baba
authors.forename: Junichi
authors.initials: J
authors.lastname: Fukui
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ueda
authors.forename: A-Hon
authors.initials: AH
authors.lastname: Kwon
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hisha
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kamiyama
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001786:Blood Glucose / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology* / Q000628:therapy*; D005951:Glucose Tolerance Test; D006085:Graft Survival / Q000276:immunology; D006648:Histocompatibility / Q000276:immunology*; D007515:Islets of Langerhans / Q000473:pathology; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D051381:Rats; D013311:Streptozocin / Q000494:pharmacology*; D013997:Time Factors; D014019:Tissue Donors; D018183:Transplantation Chimera / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D000941:Antigens; D001786:Blood Glucose; D013311:Streptozocin