title: Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome.
abstract: The double-stranded DNA genome of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) was sequenced and analysed in the context of other group I nucleopolyhedroviruses (NPVs). The genome consists of 129,593 bp with a G + C content of 50.1 mol%. A total of 146 open reading frames (ORFs) of greater than 150 bp, and with no or minimal overlap were identified. In addition, five homologous regions were identified containing 7-10 repeats of a 36 bp imperfect palindromic core. Comparison with other completely sequenced baculovirus genomes revealed that 139 of the CfMNPV ORFs have homologues in at least one other baculovirus and seven ORFs are unique to CfMNPV. Of the 117 CfMNPV ORFs common to all group I NPVs, 12 are exclusive to group I NPVs. Overall, CfMNPV is most similar to Orgyia pseudotsugata MNPV based on gene content, arrangement and overall amino acid identity. Unlike other group I baculoviruses, however, CfMNPV encodes a viral enhancing factor (vef) and has two copies of p26.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: Jondavid G
authors.initials: JG
authors.lastname: de Jong
authors.affiliation: Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada, P6A 2E5.
authors.forename: Hilary A M
authors.initials: HAM
authors.lastname: Lauzon
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: Cliff
authors.initials: C
authors.lastname: Dominy
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: Arkadi
authors.initials: A
authors.lastname: Poloumienko
authors.affiliation: Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada, K7L 3N6.
authors.forename: Eric B
authors.initials: EB
authors.lastname: Carstens
authors.affiliation: Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada, P6A 2E5.
authors.forename: Basil M
authors.initials: BM
authors.lastname: Arif
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins