title: Echinococcus granulosus: pre-culture of protoscoleces in vitro significantly increases development and viability of secondary hydatid cysts in mice.
abstract: We describe a method for obtaining improved secondary infections of Echinococcus granulosus that involves culturing protoscolex larvae in vitro prior to inoculation into mice. This approach provides a far superior method for obtaining secondary echinococcosis infections in mice compared with the traditional method of direct inoculation of protoscoleces (PSC) where the majority of parasites are killed by the host. We obtained a high rate of recovery both in terms of secondary cyst numbers and their viability. After 50 weeks post-infection (p.i.), brood capsules were formed and the first PSC developed in each of the capsules. After 56 weeks p.i., the fastest developing brood capsule contained four PSC. The approach will prove valuable for investigating parasite development and the host-parasite interaction in secondary echinococcosis.
authors.affiliation: Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, Qld 4029, Australia.
authors.forename: Wen-Bao
authors.initials: WB
authors.lastname: Zhang
authors.forename: Malcolm K
authors.initials: MK
authors.lastname: Jones
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
mesh_terms: D000818:Animals; D004443:Echinococcosis / Q000276:immunology / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D006790:Host-Parasite Interactions; D051379:Mice; D008807:Mice, Inbred BALB C