title: [Expression and significance of mRNA for MIP-1alpha in cerebral tissue of newborn rat with hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To observe the expression of mRNA for the MIP-1alpha in cerebral tissue after hypoxic-ischemic brain damage (HIBD) in newborn rat.
  
  
  METHODS
  The dynamic changes of expression of MIP-1alpha mRNA were studied using 7 d-old SD rat hypoxic-ischemic brain damage model and quantitative, real-time, fluorogenic PCR (TaqMan) assay.
  
  
  RESULTS
  Peaking transcription was shown at 6 hours after HIBD when MIP-1alpha mRNA level in the HI cerebral hemisphere was almost 10 times compared with that of control group. The mRNA level of 12 and 24 hours after HIBD was also greater than that of control group.
  
  
  CONCLUSIONS
  This appearance suggests that, as an inflammatory mediator, the MIP-1alpha transcription after HIBD may play important roles in the evolvement of brain damage.
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger