Effect of 5-azacytidine and procainamide on CD3-zeta chain expression in Jurkat T cells.
It has been observed that decrease of DNA methyltransferase 1 (DNMT1) activity is associated with low content of the CD3-zeta (zeta) chain in T cell receptor (TCR)/CD3 complex of T cells in systemic lupus erythematosus (SLE) patients. The CD3-zeta chain plays a pivotal role in intracellular signal transmission between TCR/CD3 complex and nuclei. The compounds 5'-azacytidine (AZC) and procainamide (PCA) belong to inhibitors of DNMT1, whose low activity correlates with increase in transcription of various genes. Using the reverse-transcription and real-time quantitative PCR (RQ-PCR) analysis, we indicated that AZC and PCA did not profoundly affect on CD3-zeta chain transcription in Jurkat T leukemia cells clone E6-1. However, the flowcytometric analysis revealed that AZC and PCA decreased intracellular contents of CD3-zeta chain in these cells in dose dependent manner. Our results suggest that decrease of DNMT1 activity may alter intracellular signal transmission without effect on transcription level of CD3-zeta chain.
Januchowski
Radosław
R
University of Medical Sciences, Department of Biochemistry and Molecular Biology, Poznań, Poland.
Jagodzinski
Paweł P
PP
D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D001374:Azacitidine / Q000494:pharmacology*; D017252:CD3 Complex / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D016133:Polymerase Chain Reaction; D011342:Procainamide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects* / Q000302:isolation & purification; D012097:Repressor Proteins / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured
D000889:Anti-Arrhythmia Agents; D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; C411744:DMAP1 protein, human; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D012097:Repressor Proteins; D011342:Procainamide; D001374:Azacitidine