title: Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography.
abstract: Electron tomography (ET) is a new technique for high resolution, three-dimensional (3D) reconstruction of pleiomorphic macromolecular complexes, such as virus components. By employing this technique, we resolved the 3D structure of Ebola virus nucleocapsid-like (NC-like) structures in the cytoplasm of cells expressing NP, VP24, and VP35: the minimum components required to form these NC-like structures. Reconstruction of these tubular NC-like structures of Ebola virus showed them to be composed of left-handed helices spaced at short intervals, which is structurally consistent with other non-segmented negative-strand RNA viruses.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Noda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sagara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D002478:Cells, Cultured; D029043:Ebolavirus / Q000648:ultrastructure*; D006801:Humans; D019251:Nucleocapsid / Q000737:chemistry*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D014758:Viral Core Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; C446938:VP24 protein, Ebola virus; C080980:VP35 protein, filovirus; D014758:Viral Core Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins