title: A case of granulomatous skin lesions caused by Mycobacterium marinum in the Campania region.
abstract: The diagnosis of cutaneous Mycobacterium marinum infection is frequently presumptive, as detection by conventional methods is difficult. We describe a patient with granulomatous skin lesions on the right dorsal hand and forearm. Histological examinations were presumptive for mycobacterium lesions. We identified Mycobacterium marinum directly in the patient's lesional skin biopsy combining polymerase chain reaction (PCR) amplification using Mycobacterium genus-specific primers, and subsequent restriction enzyme analysis enabling identification to the species level. The symptoms were no longer present after specific therapy, thereby confirming the initial diagnosis.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Microbiologia e Microbiologia Clinica, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy. marilena.galdiero@unina2.it
authors.forename: Marilena
authors.initials: M
authors.lastname: Galdiero
authors.forename: Emiliana
authors.initials: E
authors.lastname: Finamore
authors.forename: Emilia
authors.initials: E
authors.lastname: Galdiero
authors.forename: Feliciano
authors.initials: F
authors.lastname: Baldi
authors.forename: Luigi
authors.initials: L
authors.lastname: Petrillo
authors.forename: Giulio
authors.initials: G
authors.lastname: Petrillo
mesh_terms: D000328:Adult; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007558:Italy; D008297:Male; D009161:Mycobacterium; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D019910:Mycobacterium marinum / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017192:Skin Diseases, Bacterial / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes