The effect of the surface modification of titanium using a recombinant fragment of fibronectin and vitronectin on cell behavior.
The surface of titanium implants is in direct contact with host tissue and plays a critical role in determining biocompatibility. Fibronectin (FN) and vitronectin (VN) are major cell adhesive proteins found in the extracellular matrix (ECM) of various tissues, and in circulating blood. The aim of this study was to evaluate the engineered biomimetic surface of titanium by using recombinant fragment of FN(8-10) and VN(NTD) that contains the binding site for integrins. MC3T3-E1 cells seeded upon the FN(8-10)-coated titanium showed a marked increase in cell adhesion, proliferation, and differentiation over VN(NTD)-coated titanium. In addition, we confirmed that the surface properties of titanium prefer for FN(8-10) over VN(NTD) (p<0.05) in protein adhesion. These results suggest that the FN(8-10)-modified titanium surface can be used to improve the osseointegration of titanium implants by enhancing bone formation.
Ku
Young
Y
Intellectual Biointerface Engineering Center and Department of Periodontology, Seoul National University College of Dentistry, Seoul 110-768, Korea.
Chung
Chong-Pyoung
CP
Jang
Jun-Hyeog
JH
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics; D005353:Fibronectins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics; D019096:Vitronectin / Q000235:genetics*
D020099:Coated Materials, Biocompatible; D004268:DNA-Binding Proteins; D005353:Fibronectins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D019096:Vitronectin; D014025:Titanium; D000469:Alkaline Phosphatase