title: Plasticity and rigidity in adaptor protein-2-mediated internalization of the TCR:CD3 complex.
abstract: Many cell surface proteins are internalized via dileucine- or tyrosine-based motifs within their cytoplasmic domains by the heterotetrameric adaptor protein complex, AP-2. In this study we have examined how AP-2 mediates internalization of large cell surface receptors, such as the eight-chain TCR:CD3 complex. Although most receptors have a single signal that drives internalization, the TCR complex has two (D/E)xxxL(L/I) motifs and 20 YxxØ motifs. Using 293T cells, we show that AP-2 is completely dependent on both signals to mediate TCR internalization, because deletion of either completely blocks this process. Significant plasticity and redundancy were observed in the use of the YxxØ motifs, with a clear hierarchy in their use (CD3delta > CD3gamma >or= CD3zeta >> CD3epsilon). Remarkably, a single, membrane-distal YxxØ motif in CD3delta could mediate approximately 75% of receptor internalization, whereas its removal only reduced internalization by approximately 20%. In contrast, significant rigidity was observed in use of the (D/E)xxxL(L/I) motif in CD3gamma. This was due to an absolute requirement for the position of this signal in the context of the TCR complex and for a highly conserved lysine residue, K128, which is not present in CD3delta. These contrasting requirements suggest a general principle by which AP-2 may mediate the internalization of large, multichain complexes.
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Szymczak
authors.forename: Dario A A
authors.initials: DA
authors.lastname: Vignali
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000737:chemistry / Q000502:physiology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017252:CD3 Complex; D002460:Cell Line; D017124:Conserved Sequence; D004705:Endocytosis; D006801:Humans; D007930:Leucine; D021122:Protein Subunits; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D014443:Tyrosine
chemical_list: D033962:Adaptor Protein Complex 2; D017252:CD3 Complex; D021122:Protein Subunits; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D014443:Tyrosine; D007930:Leucine