Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good model of osteogenesis.
Osteogenesis in the teleost was morphologically observed using regenerating scales of goldfish. Histological observations indicated that osteoblasts around the regenerating scales on days 7 to 10 were greater in size and number than those at other stages. Therefore, further experiments were carried out to examine the activity of osteoblasts in the regenerating period. To quantify their osteoblastic activities, scales on the left side of the body were taken, and the regenerating scales were then used to measure the activities of alkaline phosphatase (ALP), a marker of osteoblasts, on days 7, 10, and 15. The ontogenic scales on the right side of the body were also collected and used to measure ALP activity on the same days. Osteoblasts at all stages of regenerating scales were more active than those in the remaining ontogenic scales. The regenerating scales on day 10 had the highest activity. Furthermore, we found that estrogen receptor (ER) mRNA was expressed in the regenerating scales because estrogen participates in osteoblastic growth and differentiation in mammals. Therefore, using a scale culture system reported previously, the estrogenic response was examined in the ontogenic and regenerating scales on day 10. The reactivity was much higher in regenerating scales, although estrogen treatment significantly activated the osteoblastic activities in both scales. We are the first to demonstrate that ER is expressed in regenerating scales and that estrogen participates in osteogenesis as it does in mammalian bone. Our findings strongly suggest that regenerating scales can be used as a model of osteogenesis in vertebrates.
Yoshikubo
Hiroaki
H
Division of Health Sciences, Graduate School of Medical Science, Faculty of Medicine, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan.
Suzuki
Nobuo
N
Takemura
Keiju
K
Hoso
Masahiro
M
Yashima
Sayaka
S
Iwamuro
Shawichi
S
Takagi
Yasuaki
Y
Tabata
Makoto J
MJ
Hattori
Atsuhiko
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology*; D006054:Goldfish / Q000378:metabolism*; D023421:Models, Animal; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D010010:Osteoclasts / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D012038:Regeneration
D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D000469:Alkaline Phosphatase