title: Quantitative microarray-based DNA-DNA hybridization assay for measuring genetic distances among bacterial species and its application to the identification of family Enterobacteriaceae.
abstract: Quantitative DNA-DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3-labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis. These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA-DNA hybridization for measuring chromosome relatedness among bacterial species.
authors.affiliation: Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd., Amagasaki, Hyogo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Amano
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusu
authors.forename: Koji
authors.initials: K
authors.lastname: Kusaba
authors.forename: Hironori
authors.initials: H
authors.lastname: Ikeda
authors.forename: Zenzo
authors.initials: Z
authors.lastname: Nagasawa
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Aoki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Shinzo
authors.initials: S
authors.lastname: Kobatake
authors.forename: Takumi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Shuuji
authors.initials: S
authors.lastname: Matsuura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics*; D004925:Escherichia / Q000145:classification / Q000235:genetics; D007709:Klebsiella / Q000145:classification / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics; D012705:Serratia / Q000145:classification / Q000235:genetics; D012760:Shigella / Q000145:classification / Q000235:genetics; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial