Photothermal image flow cytometry in vivo.
The capability of photothermal (PT) microscopy to image moving, unlabeled cells in real time in vivo is demonstrated in a study of circulating red and white blood cells in blood and lymph microvessels of rat mesentery. Potential applications of this optical tool, called PT flow cytometry, are discussed.
Zharov
Vladimir P
VP
Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA. zharovvladimirp@uams.edu
Galanzha
Ekaterina I
EI
Tuchin
Valery V
VV
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008643:Mesentery / Q000098:blood supply* / Q000166:cytology*; D008833:Microcirculation / Q000166:cytology*; D051381:Rats; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013817:Thermography / Q000295:instrumentation / Q000379:methods*; D014741:Video Recording / Q000295:instrumentation / Q000379:methods