Approaches to Scrapie diagnosis by applying immunohistochemistry and rapid tests on central nervous and lymphoreticular systems.
Comparative studies evaluating the performance of Transmissible Spongiform Encephalopathies (TSE) rapid tests (validated for Bovine Spongiform Encephalopathy samples) on Scrapie samples have not been reported widely, particularly those dealing with lymphoreticular system tissues to a much lesser extent. The main objective of this study was to compare the ability of two current rapid tests (Western blot and Luminiscence Immunoassay Prionics-Check; WB and LIA, respectively) to detect PrPsc using central nervous system as well as lymphoreticular system samples corresponding to naturally infected animals. Thirty-four Scrapie-affected sheep, 26 with clinical signs of the disease, were included in the study. Tonsil, retropharyngeal lymph node and medulla oblongata were assessed by three tests: immunohistochemistry (confirmatory test), WB and LIA (rapid tests). The conclusion which can be drawn from this study is the fact that all animals involved in the study, including those at a preclinical stage, could be diagnosed regardless of the test used (with immunohistochemistry consistently showing higher sensitivity) only when the analyses of both the central nervous system and the lymphoreticular system were considered. The choice of these tissues for routine diagnosis is, therefore, proposed as a valuable tool to highly reduce the number of undetected positive cases.
Monleón
E
E
National Reference Centre for Transmissible Spongiform Encephalopathies, University of Zaragoza, Avda. Miguel Servet 177, 50013 Zaragoza, Spain.
Monzón
M
M
Hortells
P
P
Bolea
R
R
Acín
C
C
Vargas
F
F
Badiola
J J
JJ
D000818:Animals; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D002490:Central Nervous System / Q000737:chemistry*; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000032:analysis*; D012608:Scrapie / Q000175:diagnosis / Q000453:epidemiology
D018620:PrPSc Proteins