Transplantation of neonatal porcine islets and sertoli cells into nonimmunosuppressed nonhuman primates.
A Mexican group reported transplantation of cocultured neonatal porcine islets and Sertoli cells resulting in insulin independence in nonimmunosuppressed type 1 diabetes patients. We have transplanted similar islets alone (naked islets) or cocultured islets with sertoli cells (islet/sertoli cells) into an omental site and other locations of seven nondiabetic, nonimmunosuppressed, nonhuman primates. Porcine endogenous retrovirus was not detected in recipient blood 8 weeks after porcine islet grafts, and porcine C-peptide was detected at a very low level in all animals. Histology examination failed to demonstrate obviously recognizable islets, but in the animals transplanted with islet/Sertoli cells at the omentum site, there were some surviving glucagons, pan-cytokeratin, and inhibin stained cells at 8 weeks.
Isaac
J R
JR
Department of Surgery, National University of Singapore, Singapore.
Skinner
S
S
Elliot
R
R
Salto-Tellez
M
M
Garkavenko
O
O
Khoo
A
A
Lee
K O
KO
Calne
R
R
Wang
D Z
DZ
D000818:Animals; D000831:Animals, Newborn; D006085:Graft Survival; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D008251:Macaca; D008297:Male; D012708:Sertoli Cells / Q000637:transplantation*; D013552:Swine; D014183:Transplantation, Heterologous