[The effect of transfecting Stat3beta cDNA on human breast cancer].
OBJECTIVE
To study the effect of transfecting Stat3beta cDNA on human breast cancer.


METHODS
Human breast cancer cells of the line SK-BR-3 were cultured and divided into 3 groups: Stat3beta transfection group (to be transfected with plasmid pIRES-Stat3beta containing Stat3beta by transient transfection technique), lipofectin reagent transfection group pIRES-EGFP transfection group, and control group. The positively transfected cells were isolated by fluorescence-activated cell sorter. Flow cytometry was used to analyze the cell cycles and cell apotosis. Western blotting was used to detect the expression of STAT3 protein. MTT method was used to examine the proliferation of the cells.


RESULTS
Forty-eight hours after exposure to the plasmid pIRES-Stat3beta the transfection rate of the SK-BR-3 cells was 13.79%. SK-BR-3 cells expressed STA3 protein during proliferation. In comparison with the SK-BR-3 cells of other 3 group, the proliferation of the cells transfected with pIRES-Stat3beta was significantly decreased. Forty-eight hours after transfection, 81.09% of the cells transfected with the plasmid pIRES-Stat3beta accumulated at the G(0)/G(1) stage, a rate significantly higher than those of the other groups, and displayed a significantly higher rate of apoptosis.


CONCLUSIONS
Transfection of plasmid pIRES-Stat3beta containing Stat3beta blocks the Stat3 pathway, thus inhibiting the proliferation and augment the apoptosis of human breast cancer cells and providing a novel gene therapy target.
Cheng
Lin
L
Department of Breast Surgery and Laboratory of Surgical Oncology, Peking University People's Hospital, Beijing 100044, China.
Wang
Shan
S
Ye
Ying-Jiang
YJ
Zhang
Jia-Qing
JQ
Qiao
Xin-Min
XM
Yang
De-Qi
DQ
Cui
Zhi-Rong
ZR
D000209:Acute-Phase Proteins; D017209:Apoptosis / Q000502:physiology*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D018076:DNA, Complementary; D015316:Genetic Therapy; D006801:Humans; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction; D014162:Transfection
D000209:Acute-Phase Proteins; D018076:DNA, Complementary; D050796:STAT3 Transcription Factor