title: Thrombelastograph (TEG) analysis of platelet gel formed with different thrombin concentrations.
abstract: Autologous blood transfusion is the safest and most successful way to decrease transfusion-related risks such as postoperative infections, allo-immunization, and short- and long-term immunosuppression. In addition, these fibrin sealants are known to provide coagulation support at the surgical site and act as an adjunct to the control of postoperative bleeding. The physical formation of autologous platelet fibrin gel clot is dependent on both the common pathway of the coagulation cascade and platelet activation. Platelet gel can help provide control of intraoperative and postoperative bleeding. The Thrombelastograph Hemostasis Analyzer (TEG) measures the viscoelastic properties of a clot as it forms. Based on the information that the TEG provides, it promises to be a good choice for point of care measurement of the integrity of thrombus formed by platelet gels. Bovine blood from a single donor was sequestered into platelet-rich plasma and was made into platelet gel using calcium and three different concentrations of thrombin. The platelet gel samples were then analyzed with the TEG analyzer. The results for MA, tMA, CI, and angle were recorded and statistical analysis was performed to accept or reject the null hypothesis, which is: There is no difference between TEG parameters when analyzing platelet gels formed with calcium chloride, platelet-rich plasma and three different concentrations of thrombin A one-way analysis of variance test was performed between thrombin concentrations for MA (p = 0.19), tMA (p = 0.443), CI (p = 0.257), and angle (p = 0.323). The results showed that thrombin concentration did not affect the MA, tMA, CI, or angle as measured by the TEG analyzer. The null hypothesis was accepted. Based on a one-way analysis of variance test for MA, tMA, CI, and angle there was no significant statistical difference for the TEG samples in this experiment as reported with a 95% confidence interval.
authors.affiliation: Circulation Technology Division, The Ohio State University School of Allied Medical Professions, Columbus, Ohio, USA. ellis.287@osu.edu
authors.forename: William Cory
authors.initials: WC
authors.lastname: Ellis
authors.forename: Linsay K
authors.initials: LK
authors.lastname: Cassidy
authors.forename: Angela S
authors.initials: AS
authors.lastname: Finney
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Spiwak
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Riley
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D001792:Blood Platelets; D002417:Cattle; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D005782:Gels; D066298:In Vitro Techniques; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D013916:Thrombelastography / Q000295:instrumentation*; D013917:Thrombin
chemical_list: D015718:Fibrin Tissue Adhesive; D005782:Gels; D013917:Thrombin