Reducing contralateral SI activity reveals hindlimb receptive fields in the SI forelimb-stump representation of neonatally amputated rats.
In adult rats that sustained forelimb amputation on the day of birth, >30% of multiunit recording sites in the forelimb-stump representation of primary somatosensory cortex (SI) also respond to cutaneous hindlimb stimulation when cortical GABA(A+B) receptors are blocked (GRB). This study examined whether hindlimb receptive fields could also be revealed in forelimb-stump sites by reducing one known source of excitatory input to SI GABAergic neurons, the contralateral SI cortex. Corpus callosum projection neurons connect homotopic SI regions, making excitatory contacts onto pyramidal cells and interneurons. Thus in addition to providing monosynaptic excitation in SI, callosal fibers can produce disynaptic inhibition through excitatory synapses with inhibitory interneurons. Based on the latter of these connections, we hypothesized that inactivating the contralateral (intact) SI forelimb region would "unmask" normally suppressed hindlimb responses by reducing the activity of SI GABAergic neurons. The SI forelimb-stump representation was first mapped under normal conditions and then during GRB to identify stump/hindlimb responsive sites. After GRB had dissipated, the contralateral (intact) SI forelimb region was mapped and reversibly inactivated with injections of 4% lidocaine, and selected forelimb-stump sites were retested. Contralateral SI inactivation revealed hindlimb responses in approximately 60% of sites that were stump/hindlimb responsive during GRB. These findings indicate that activity in the contralateral SI contributes to the suppression of reorganized hindlimb receptive fields in neonatally amputated rats.
Pluto
Charles P
CP
Department of Anatomy and Neurobiology, Medical Collegeo of Ohio, 3000 Arlington Ave., Toledo, Ohio 43614, USA. cpluto@mco.edu
Chiaia
Nicolas L
NL
Rhoades
Robert W
RW
Lane
Richard D
RD
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000344:Afferent Pathways / Q000503:physiopathology; D000672:Amputation Stumps / Q000503:physiopathology*; D000704:Analysis of Variance; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology; D001931:Brain Mapping; D004347:Drug Interactions; D005552:Forelimb / Q000503:physiopathology*; D007839:Functional Laterality / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D035002:Lower Extremity / Q000294:innervation / Q000503:physiopathology*; D008959:Models, Neurological; D051381:Rats; D013003:Somatosensory Cortex / Q000187:drug effects / Q000503:physiopathology*
D000779:Anesthetics, Local; D018756:GABA Antagonists; D008012:Lidocaine; D001640:Bicuculline