[Influence of cryoprotectant with glycerol and freezing-thawing procedure on the motility of human sperm].
OBJECTIVE
To investigate the effects of cryoprotectant with glycerol and the freezing-thawing procedure on the motility of


METHODS
The motion characteristics of human sperm from 18 selected specimens were assessed by computer-assisted human sperm. sperm analysis before and after adding hyper-osmolarity cryoprotectant with glycerol and the freeze-thaw procedure, and the data were evaluated in pairs.


RESULTS
The adding of cryoprotectant caused an increase in the proportion of rapid linear motile sperms (P < 0.05) and sperm velocities, including VCL, VSL and VAP (P < 0.005). But no changes were observed in the proportion of progressively motile sperms and sperm motility. Compared with the data from pre-frozen samples, velocities of post-thawed sperms and the percentage of motile sperms in each grade significantly declined (P < or = 0.01), so did ALH, while WOB, LIN and STR remained unchanged (P > 0.05). Significant differences in ALH, WOB, LIN and STR were observed only in comparison between the post-thawed and pre-treated samples.


CONCLUSIONS
The number of Grade a sperms and sperm velocity increased after adding hyper-osmolarity cryoprotectant with glycerol. The sperm motile potential was impaired and even entirely destroyed in some cases by cryodamage. The influences on the sperm motion were differently induced in freezing-thawing procedures.
Yue
Huan-xun
HX
Human Sperm Bank, Huaxi Second Hospital of Sichuan University, Chengdu, Sichuan 610041, China. huanxuny@vip.139sc.com
Li
Fu-ping
FP
Jiang
Min
M
Lin
Li
L
Xu
Ke-hui
KH
D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D006801:Humans; D008297:Male; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects*
D003451:Cryoprotective Agents; D005990:Glycerol