title: Application of SSB-like protein from Thermus aquaticus in multiplex PCR of human Y-STR markers identification.
abstract: Here, we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in multiplex PCR of human Y-STR markers identification. The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction of a number of primers available for annealing in PCR reaction.
authors.affiliation: Department of Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-952 Gdańsk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Olszewski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Rebała
authors.forename: Zofia
authors.initials: Z
authors.lastname: Szczerkowska
authors.forename: Józef
authors.initials: J
authors.lastname: Kur
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*; D013824:Thermus
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins