Optimized DNA targeting using N,N-bis(2-pyridylmethyl)-beta-alanyl 2'-amino-LNA.
Incorporation of N,N-bis(2-pyridylmethyl)-beta-alanyl 2'-amino-LNA (bipyridyl-functionalized 2'-amino locked nucleic acid) monomers into DNA strands enables high-affinity targeting of complementary DNA with excellent Watson-Crick selectivity in the presence of divalent metal ions. Positioning of bipyridyl-functionalized 2'-amino-LNA monomers in two complementary DNA strands in a "3'-end zipper" constitution allows modulation of duplex stability, i.e., a strong stabilizing effect with one equivalent of divalent metal ion per bipyridyl pair, or a strong destabilizing effect with an excess of divalent metal ions.
Babu
B Ravindra
BR
Nucleic Acid Center, I Department of Chemistry, University of Southern Denmark, DK-5230, Odense M, Denmark.
Hrdlicka
Patrick J
PJ
McKenzie
Christine J
CJ
Wengel
Jesper
J
D000409:Alanine / Q000737:chemistry*; D000586:Amination; D002413:Cations, Divalent / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry; D008745:Methylation; D008958:Models, Molecular; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D011725:Pyridines / Q000737:chemistry*; D044366:Transition Temperature
D002413:Cations, Divalent; D019216:Metals, Heavy; D011725:Pyridines; D004247:DNA; C023666:pyridine; D000409:Alanine