title: Enzymatic synthesis of oligosaccharides from branched cyclodextrins.
abstract: 6''-alpha-Maltosyl-maltotriose and 6''-alpha-D-glucosyl-maltotriose were prepared from Novamyl degradation of 6-O-alpha-maltosyl-alpha-cyclodextrin and 6-O-alpha-D-glucosyl-alpha-cyclodextrin, respectively. NMR spectroscopy was used to elucidate their structural identities, in a combination of COSY experiments. Further, a mechanism for the degradation was proposed based on the Novamyl active site geometry.
authors.affiliation: Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark.
authors.forename: Christel T
authors.initials: CT
authors.lastname: Jørgensen
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.forename: Jesper
authors.initials: J
authors.lastname: Brask
mesh_terms: D002240:Carbohydrate Sequence; D003505:Cyclodextrins / Q000737:chemistry* / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000378:metabolism*
chemical_list: D003505:Cyclodextrins; D004798:Enzymes; D009844:Oligosaccharides