title: Molecular insights into the initiation of sporulation in Gram-positive bacteria: new technologies for an old phenomenon.
abstract: The last decade has witnessed extensive, and widespread, changes in scientific technologies that have impacted significantly upon the study of the life sciences. Arguably, the biggest advances in our comprehension of simple and complex biological processes have come as a consequence of obtaining the complete DNA sequence of organisms. It is likely that we will become accustomed to hearing of quantum leaps in the study and understanding of the biology of higher eukaryotes in the coming years, now that (near) complete genome sequences are available for man, mouse and rat. In this review, we will discuss the impact of genome sequence data, and the use of new scientific technologies that have emerged largely as consequence of the availability of this information, on the study of the master regulator of sporulation, Spo0A, in low G+C Gram-positive endospore-forming bacteria.
authors.affiliation: School of Biochemistry and Microbiology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. r.lewis@ncl.ac.uk
authors.forename: Keith
authors.initials: K
authors.lastname: Stephenson
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Lewis
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D023281:Genomics; D016937:Gram-Positive Endospore-Forming Rods / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008958:Models, Molecular; D040901:Proteomics; D015398:Signal Transduction; D013171:Spores, Bacterial / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors