[Study on technique of extraction and purifying chlorogenic acid in Eucommia ulmoides Oliver leaves].
Ethanol was used as solvent to extract chlorogenic acid from leaves of Eucommia ulmoides Oliver leaves. The optimum conditions are obtained by response surface analytical method. Optimized conditions for the extraction of chlorogenic acid were obtained: ethanol 51.7%, extraction temperature 55.8 degrees C, ratio of material to liquor 1:12.8, extraction time 2 h. The static adsorption tests are conducted among six kinds of macroporous resins to select NKA-II resin as a optimum resin to adsorb the chlorogenic acid in Eucommia ulmoides Oliver leaves. The optimum adsortion-elution conditions are as follow: adsorption flow rate is 2 ml/min, crude liquid flew twice through the adsorption column,the optimum adsorbent is 40% ethanol.
Liu
Junhai
J
School of Food and Technology, Jiangnan University, Wuxi.
Qiu
Aiyong
A
D000327:Adsorption; D000837:Anion Exchange Resins; D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D031283:Eucommiaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
D000837:Anion Exchange Resins; D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid