Interaction of the conserved meiotic regulators, BOULE (BOL) and PUMILIO-2 (PUM2).
Germ cell development is complex; it encompasses specification of germ cell fate, mitotic replication of early germ cell populations, and meiotic and postmeiotic development. Meiosis alone may require several hundred genes, including homologs of the BOULE (BOL) and PUMILIO (PUM) gene families. Both BOL and PUM homologs encode germ cell specific RNA binding proteins in diverse organisms where they are required for germ cell development. Here, we demonstrate that human BOL forms homodimers and is able to interact with a PUMILIO homolog, PUM2. We mapped the domain of BOL that is required for dimerization and for interaction with PUM2. We also show that BOL and PUM2 can form a complex on a subset of PUM2 RNA targets that is distinct from targets bound by PUM2 and another deleted in azoospermia (DAZ) family member, DAZ-like (DAZL). This suggests that RNA sequences bound by PUM2 may be determined by protein interactions. This data also suggests that although the BOL, DAZ, and DAZL proteins are all members of the same gene family, they may function in distinct molecular complexes during human germ cell development.
Urano
Jun
J
Program in Human Stem Cell Biology, Department of Obstetrics, Center for Reproductive Sciences, and Program in Human Genetics, University of California, San Francisco, California 94143-0556, USA. reijo@itsa.ucsf
Fox
Mark S
MS
Reijo Pera
Renee A
RA
D002460:Cell Line; D006801:Humans; D008297:Male; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*
C481351:BOLL protein, human; C471243:PUM2 protein, human; D016601:RNA-Binding Proteins