Enzyme-linked immunosorbent assay for the detection of canine Leptospira antibodies using recombinant OmpL1 protein.
OmpL1 is a 31-kDa outer membrane protein characterized in 1993 and known to be expressed only in pathogenic Leptospira spp. Recombinant OmpL1 (GST-rOmpL1) was expressed for use as an ELISA antigen for the detection of anti-Leptospira antibodies. In immunoblot analysis, the protein reacted with sera of dogs infected with three different serotypes of Leptospira interrogans, while did not react with sera of dogs both uninfected negative controls and infected with Borrelia burgdorferi, which is closely related to Leptospira spp. Moreover, in ELISA using GST-rOmpL1, the optical density (O.D.) values from the positive controls were very high (1.125 +/- 0.549). In contrast, the O.D. values from clinically healthy dogs and dogs with diseases other than leptospirosis were very low (0.109 +/- 0.046 and 0.089 +/- 0.046, respectively). These data suggest that the detection of anti-Leptospira antibodies by ELISA using the GST-rOmpL1 protein can be applied for diagnosis of canine leptospirosis.
Okuda
Masaru
M
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Sakai
Yoshiko
Y
Matsuuchi
Megumi
M
Oikawa
Tatsuo
T
Watanabe
Malaika
M
Itamoto
Kazuhito
K
Iwata
Hiroyuki
H
Kano
Rui
R
Hasegawa
Atsuhiko
A
Onishi
Takafumi
T
Inokuma
Hisashi
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000378:metabolism
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; C081904:ompL1 protein, Leptospira