[The effect of "Jian Pi Dao Zhi Fang" on human gastric carcinoma cells].
OBJECTIVE
To study the effect of Traditional Chinese medicine "Jian Pi Dao Zhi Fang" (JPDZF) with invigorating the spleen and purgative function on the growth of gastric carcinoma cells and the expression of VEGF and P53 in gastric carcinoma cells.


METHODS
Gastric carcinoma cells were cultured in the RPMI-1640 media with 10% serum containing JPDZF for 48 hours. The cell cycle were tested with flow cytometer. Expression of P53, VEGF and its receptor Fit were evaluated by immunohistochemistry.


RESULTS
The cell cycle of gastric carcinoma cells cultured in the media with JPDZF were different from the control. The expression of P53, VEGF, Flt decreased with the dosage increasing of JPDZF.


CONCLUSIONS
Traaditional Chinese medicine JPDZF can inhibit the growth of gastric carcinoma cells and the expression of P53, VEGF, Flt.
Min
Cunyun
C
Guangdong Provincial Pepople's Hospital, Guangzhou.
Li
Qingming
Q
Liu
Heqiang
H
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D000970:Antineoplastic Agents; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A