title: The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes.
abstract: Polyelectrolyte complexes (PEC) were prepared from chitosan as the polycation and several synthesized functional anion polysaccharides, and their effects on cell attachment, morphology, proliferation and differentiation were estimated using normal human osteoblasts (NHOst). After a 1-week incubation, PEC made from polysaccharides having carboxyl groups as polyanions showed low viability of NHOst on it although the NHOst on it showed an enhancement in their differentiation level. On the other hand, NHOst on PEC made from sulfated or phosphated polysaccharides showed similar attachment and morphology to those on the collagen-coated dish. When the number of NHOst was estimated after 1 week, the number on the PEC was ranged from 70% to 130% of those on the collagen-coated dish, indicating few effects of these PEC on cell proliferation. In addition, NHOst on PEC films made from sulfated polysaccharides differentiated to a level very similar to that observed on the collagen-coated dish, indicating that these PEC films maintain the normal potential of NHOst to both proliferate and differentiate. Measurement of gap junctional intercellular communication of NHOst on PEC revealed that PEC did not inhibit communication, suggesting that PEC films have few effects on cell homeostasis. Thus, PEC made from the sulfated polysaccharide may be a useful material as a new scaffold for bone regeneration.
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, 1-81-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. nagahata@nihs.go.jp
authors.forename: Misao
authors.initials: M
authors.lastname: Nagahata
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Nakaoka
authors.forename: Akira
authors.initials: A
authors.lastname: Teramoto
authors.forename: Koji
authors.initials: K
authors.lastname: Abe
authors.forename: Toshie
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D036681:Fluorescence Recovery After Photobleaching; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D000469:Alkaline Phosphatase; D002118:Calcium; D002686:Chitin; D048271:Chitosan; D006820:Hyaluronic Acid; D046911:Macromolecular Substances; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D011134:Polysaccharides