title: The overexpression of an intracellular ABCA-like transporter alters phospholipid trafficking in Leishmania.
abstract: In parasites, ATP-binding cassette (ABC) transporters represent an important family of proteins related to drug resistance and other biological activities. Here we report the characterization of LtrABCA2, a new ABC transporter of the ABCA subfamily in the protozoan parasite Leishmania tropica, localized at the flagellar pocket region and in internal vesicles. The overexpression of this transporter reduced the accumulation of fluorescent glycerophospholipid analogs, increased the exocytic activity, and decreased infectivity of macrophage, but did not confer resistance to drugs. Together, these results suggest that this new ABC transporter plays a role in phospholipid trafficking, which may be modifying the vesicular trafficking and the infectivity of the parasite.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, C.S.I.C, Parque Tecnológico de Ciencias de la Salud, Avda del Conocimiento s/n 18100 Armilla, Granada, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Araújo-Santos
authors.forename: Adriana
authors.initials: A
authors.lastname: Parodi-Talice
authors.forename: Santiago
authors.initials: S
authors.lastname: Castanys
authors.forename: Francisco
authors.initials: F
authors.lastname: Gamarro
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005089:Exocytosis; D019084:Fluorescent Antibody Technique, Indirect; D007895:Leishmania tropica / Q000378:metabolism* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D010743:Phospholipids