Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.
Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.
Digman
Michelle A
MA
Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Sengupta
Parijat
P
Wiseman
Paul W
PW
Brown
Claire M
CM
Horwitz
Alan R
AR
Gratton
Enrico
E
D000465:Algorithms; D000818:Animals; D001703:Biophysics / Q000379:methods; D002460:Cell Line; D006224:Cricetinae; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015233:Models, Statistical; D016011:Normal Distribution; D017785:Photons; D013997:Time Factors
D049452:Green Fluorescent Proteins