Augmentation of tissue ATP level during digestion using preoxygenated perfluorocarbon results in high islet yield-new strategy for islet isolation.
BACKGROUND
Recently, preservation using oxygenated perfluorocarbon (the two-layer method) has shown beneficial effects on islet yield and viability. In this paper, we apply this concept on isolation processes to examine the effectiveness of oxygenation.


METHODS
Rat pancreata were digested using four different methods: (groups 1A, 1B, 2A, and 2B) with or without oxygenated perfluorocarbon in groups 1 and 2, respectively. Adenosine was added into the collagenase solution in subgroup A whereas it is not added in subgroup B.


RESULTS
Tissue oxygen tension in group 1 was about 0 during digestion; whereas it rapidly reached about 300 mm Hg and was maintained in group 2. Tissue ATP level just after laparotomy (control) was 4.2 +/- 0.7 micromol/g dry weight. The ATP levels after digestion were 0.12 +/- 0.03 in group 1A (P < 0.01 vs control); 0.70 +/- 0.10 in group 1B (P < 0.01 vs control); 0.30 +/- 0.18 in group 2A (P < 0.01 vs control); and 2.90 +/- 0.80 in group 2B (P = 0.19 vs control). Islet yields (IEQ/pancreas) were 1600 +/- 400 in group 1B; 1400 +/- 400 in group 1B; 1300 +/- 400 in group 2A; and 2400 +/- 100 in group 2B. The amount in group 2B was significantly greater than that in the other three groups.


CONCLUSIONS
Oxygen provision by preoxygenated perfluorocarbon itself showed no beneficial effect on islet yield. However, if oxygen provision was associated with adenosine administration into the pancreas, tissue ATP levels after digestion were well maintained, and a greater number of islets were retrieved.
Tanioka
Y
Y
Division of Gastrointestinal Surgery, Graduate School of Medicine, Kobe University, Kobe, Hyogo, Japan.
Tanaka
T
T
Gotoh
T
T
Kakinoki
K
K
Li
S
S
Yoshikawa
T
T
Sakai
T
T
Fujino
Y
Y
Suzuki
Y
Y
Kuroda
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation / Q000379:methods; D005466:Fluorocarbons / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption; D010180:Pancreatectomy; D051381:Rats; D011917:Rats, Inbred Lew
D005466:Fluorocarbons; D000255:Adenosine Triphosphate