Ex vivo amplification of T cells from human cord blood.
The current status of immunotherapy for cancer is here summarized with particular attention to the new methodologies developed for ex vivo expansion of T cells from neonatal cord blood. Umbilical cord blood (UCB) mononuclear cells (MNC) generate CD45RA naive T lymphocytes when cultured under serum-deprived conditions with appropriate combinations of growth factors. These ex vivo generated T cells resemble precursors for the lymphoid lineage present in adult bone marrow in terms of active transcription of RAG-2 and pTalpha.
Migliaccio
Anna Rita
AR
Laboratory of Clinical Biochemistry, Istituto Superiore di Sanità, Rome, Italy. migliagi@iss.it
Alfani
Elena
E
Di Giacomo
Viviana
V
Cieri
Monia
M
Migliaccio
Giovanni
G
D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood; D001854:Bone Marrow Cells / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
D015703:Antigens, CD; D018952:Antigens, CD34; D006133:Growth Substances