Efficiency of magnetic liposomal transforming growth factor-beta 1 in the repair of articular cartilage defects in a rabbit model.
We evaluated the efficacy of a magnetic liposomal delivery system of transforming growth factor (TGF)-beta(1) in the treatment of articular cartilage defects in a rabbit model. Articular cartilage defects were created in the patellar groove of rabbits, and a permanent magnet or a nonmagnetic alloy was implanted in the defect site. Magnetic liposomal drugs, prepared by the conventional film method and sonication, were injected into the defect site 1 week after surgery. First, the efficacy of the magnetic liposomal delivery system was evaluated by using a model compound fluorescence-labeled dextran 40,000 (FD-40). Then, the therapeutic efficiency of magnetic liposomal TGF-beta(1) was evaluated by cartilage histological scoring at 4, 8, and 12 weeks after surgery. The injected magnetic liposomal FD-40 accumulated at the target site where a permanent magnet had been implanted. The histological score showed that the injection of magnetic liposomal TGF-beta(1) under magnetic force was significantly effective in the repair of the defect site over 12 weeks after surgery. Injection of TGF-beta(1) into the cartilage defect was effective as a magnetic liposomal preparation under magnetic force, resulting in acceleration of the cartilage repair, probably because of the desirable accumulation of TGF-beta(1) at the target site.
Tanaka
Haruyuki
H
Department of Orthopaedic Surgery, Programs for Applied Biomedicine, Division of Clinical Medical Science, Hiroshima University, Minami-ku, Japan. haltan-b@r6.dion.ne.jp
Sugita
Takashi
T
Yasunaga
Yuji
Y
Shimose
Shouji
S
Deie
Masataka
M
Kubo
Tadahiko
T
Murakami
Teruo
T
Ochi
Mitsuo
M
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage / Q000378:metabolism*; D008280:Magnetics; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
D008081:Liposomes; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1