Leupaxin binds to PEST domain tyrosine phosphatase PEP.
PEST domain tyrosine phosphatase (PEP) is an intracellular protein tyrosine phosphatase and characterized by PEST motifs and proline-rich domains in the carboxyl terminal half. PEP is primarily expressed in hematopoietic cells, and together with PEP-binding Csk, may act as a negative regulator of antigen receptor signaling in lymphocytes. Here, we show the binding capability of PEP for leupaxin, which is preferentially expressed in hematopoietic cells and a comparatively new member of the paxillin family characterized by two protein-protein interaction modules, LIM domains and LD motifs. These results suggested that leupaxin might participate in the regulation of the signaling cascade through the binding to PEP in lymphocytes.
Watanabe
Noriyuki
N
Department of Life Science and Frontier Project "Life's Adaptation Strategies to Environmental Changes," Rikkyo (St Paul's) University, Nishi-Ikebukuro, Toshima-ku, Tokyo, Japan.
Amano
Natsuko
N
Ishizuka
Hajime
H
Mashima
Keisuke
K
D020816:Amino Acid Motifs; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
D015815:Cell Adhesion Molecules; C112251:LPXN protein, human; D010750:Phosphoproteins; C516738:PTPN12 protein, human; D054594:Protein Tyrosine Phosphatase, Non-Receptor Type 12; D017027:Protein Tyrosine Phosphatases