title: Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
abstract: OBJECTIVE
  To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
  
  
  METHODS
  A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
  
  
  RESULTS
  Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
  
  
  CONCLUSIONS
  SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes.
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Cui
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Liu
mesh_terms: D000818:Animals; D001201:Ascites / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000556:secondary*; D045744:Cell Line, Tumor; D015252:Deoxyribonucleases, Type II Site-Specific; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000556:secondary*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases