title: Induction of CD69 antigen expression in peripheral blood mononuclear cells on exposure to silica, but not by asbestos/chrysotile-A.
abstract: While cases of silicosis are often complicated by various autoimmune disorders, patients with asbestosis develop malignant tumors such as lung cancer and malignant mesothelioma. These differences may derive from different biological effects, particularly on immunological cells, of silica and asbestos. To find differences between silica and asbestos, the early activation antigen, CD69, on T cells was examined because dysregulated and continuous activation of T cells may promote the survival of self-recognizing T cells. After cultivation of peripheral blood mononuclear cells with or without silica or chrysotile-A, an asbestos, only silica induced CD69 expression on the lymphocytes. This induction of CD69 expression was mediated by protein kinase C activation. In addition, cell-cell contact mediated by HLA-DR was more important than soluble factors secreted from silica-phagocytosed cells such as IL-1beta, IL-6, and IL-8, even though IL-6 and IL-8 were produced during the culture of PBMCs with silica and chrysotile-A. It should be examined how these activated, CD69-expressing lymphocytes affect other immune systems as well as alter themselves in terms of cytokine production and cell-cell interaction, leading to autoimmune disorders in silicosis patients.
authors.affiliation: Department of Hygiene, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Fuminori
authors.initials: F
authors.lastname: Hyodoh
authors.forename: Tamayo
authors.initials: T
authors.lastname: Hatayama
authors.forename: Haruko
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Satomi
authors.initials: S
authors.lastname: Hatada
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Miura
authors.forename: Akiko
authors.initials: A
authors.lastname: Takata-Tomokuni
authors.forename: Hironobu
authors.initials: H
authors.lastname: Katsuyama
authors.forename: Takemi
authors.initials: T
authors.lastname: Otsuki
mesh_terms: D000906:Antibodies; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D017632:Asbestos, Serpentine / Q000494:pharmacology*; D018920:Coculture Techniques; D006801:Humans; D037181:Lectins, C-Type; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D000099192:CD69 Antigens
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017632:Asbestos, Serpentine; D037181:Lectins, C-Type; D011493:Protein Kinase C; D000099192:CD69 Antigens