title: Effect of adding dietary L-lysine, L-threonine and L-methionine to a low gluten diet on urea synthesis in rats.
abstract: We have shown that urinary urea excretion increased in rats fed a low quality protein. The purpose of present study was to determine whether an addition of dietary limiting amino acids affected urea synthesis in rats fed a low gluten diet. Experiments were done on three groups of rats given diets containing 10% gluten, 10% gluten +0.5% L-lysine or 10% gluten+0.5% L-lysine, 0.2% L-threonine and 0.2% L-methionine for 10 d. The urinary excretion of urea, and the liver concentrations of serine and ornithine decreased with the addition of dietary L-lysine, L-threonine and L-methionine. The fractional and absolute rates of protein synthesis in tissues increased with the treatment of limiting amino acids. The activities of hepatic urea-cycle enzymes was not related to the urea excretion. These results suggest that the addition of limiting amino acids for the low gluten diet controls the protein synthesis in tissues and hepatic ornithine and decline urea synthesis.
authors.affiliation: Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tujioka
authors.forename: S
authors.initials: S
authors.lastname: Lyou
authors.forename: Y
authors.initials: Y
authors.lastname: Fukaya
authors.forename: A
authors.initials: A
authors.lastname: Sano
authors.forename: K
authors.initials: K
authors.lastname: Hayase
authors.forename: H
authors.initials: H
authors.lastname: Yokogoshi
mesh_terms: D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D005983:Glutens / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism; D008239:Lysine / Q000008:administration & dosage*; D008297:Male; D008715:Methionine / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013912:Threonine / Q000008:administration & dosage*; D014508:Urea / Q000652:urine*
chemical_list: D013912:Threonine; D005983:Glutens; D014508:Urea; D008715:Methionine; D008239:Lysine