Synthetic wogonin derivatives suppress lipopolysaccharide-induced nitric oxide production and hydrogen peroxide-induced cytotoxicity.
Wogonin (5,7-dihydroxy-8-methoxyflavone) has been reported to exhibit a variety of biological properties including anti-inflammatory and neuroprotective functions. In this study, biological activities of diverse synthetic wogonin derivatives have been evaluated in two experimental cell culture models. Inhibitory activities of wogonin derivatives on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 microglial cells and on hydrogen peroxide (H2O2)-induced neuronal cell death in SH-SY5Y human neuroblastoma were examined. Wogonin derivatives such as WS2 and WS3 showed more potent suppressive activities on LPS-induced NO production and H2O2-induced cytotoxicity than wogonin itself. In addition, thiol substitution played a minor role in enhancing the activities of the derivatives. These findings may contribute to the development of novel anti-inflammatory and neuroprotective agents derived from wogonin.
Chun
Wanjoo
W
Department of Pharmacology, Kangwon National University, Chuncheon 200-701, Korea.
Lee
Hee Jae
HJ
Kong
Pil-Jae
PJ
Lee
Gun Hee
GH
Cheong
Il-Young
IY
Park
Haeil
H
Kim
Sung-Soo
SS
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D014407:Tumor Cells, Cultured
D044950:Flavanones; D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D016877:Oxidants; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C085514:wogonin