title: Cytotoxicity of Fusarium mycotoxins to mammalian cell cultures as determined by the MTT bioassay.
abstract: Fusarium mycotoxins occur worldwide in cereal grains and animal feeds and cause outbreaks of Fusarium mycotoxicoses in humans and animals. In this study mammalian cell cultures were used to screen the cytotoxicity of the most common Fusarium mycotoxins; deoxynivalenol (DON), zearalenone (ZEN), fumonisin B(1) (FB(1)) and moniliformin (MON). The most sensitive cell line for each Fusarium mycotoxin was determined for further toxicological investigations as an alternative to whole animal testing. Chinese hamster ovary cells (CHO-K1) were found to be the most sensitive for DON and FB(1) with IC(50) values of 0.27 and 85.5 microg/ml, respectively, after 48-h exposure. The hepatocellular carcinoma cells (HepG2) showed the highest sensitivity to MON with IC(50) values of 39.5 for 48 h and 26.8 microg/ml for 72-h exposure. Balb/c mice keratinocyte cell line (C5-O) was found to be the most sensitive to ZEN with IC(50) of 24.1 microg/ml after 72-h exposure. DON was found the most cytotoxic to the cell cultures of all the mycotoxins tested, followed by MON, ZEN, and FB(1). The results indicated that CHO-K1, C5-O, and HepG2 cells were found to be the sensitive cell lines for preliminary screening of DON, ZEN and MON contaminated feed and food extracts, respectively.
authors.affiliation: Department of Food Science and Technology, University of Nebraska-Lincoln, 143 Filley Hall, Lincoln, NE 68583-0919, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Cetin
authors.forename: L B
authors.initials: LB
authors.lastname: Bullerman
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000382:microbiology; D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002460:Cell Line; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D003503:Cyclobutanes / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000633:toxicity; D005670:Fusarium / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000633:toxicity*; D013997:Time Factors; D014255:Trichothecenes / Q000633:toxicity; D015025:Zearalenone / Q000633:toxicity
chemical_list: D003503:Cyclobutanes; D037341:Fumonisins; D009183:Mycotoxins; D014255:Trichothecenes; C008539:moniliformin; C056933:fumonisin B1; D015025:Zearalenone; C007262:deoxynivalenol