Enzymatic synthesis of oligosaccharides from branched cyclodextrins.
6''-alpha-Maltosyl-maltotriose and 6''-alpha-D-glucosyl-maltotriose were prepared from Novamyl degradation of 6-O-alpha-maltosyl-alpha-cyclodextrin and 6-O-alpha-D-glucosyl-alpha-cyclodextrin, respectively. NMR spectroscopy was used to elucidate their structural identities, in a combination of COSY experiments. Further, a mechanism for the degradation was proposed based on the Novamyl active site geometry.
Jørgensen
Christel T
CT
Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark.
Svendsen
Allan
A
Brask
Jesper
J
D002240:Carbohydrate Sequence; D003505:Cyclodextrins / Q000737:chemistry* / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000378:metabolism*
D003505:Cyclodextrins; D004798:Enzymes; D009844:Oligosaccharides