Evidence of an anti-inflammatory role for Vasogen's immune modulation therapy.
We have reported that Vasogen's immune modulation therapy (IMT), a procedure involving intramuscular administration of autologous/syngeneic blood, which has been exposed ex vivo to increased temperature, UVC light and oxidation, prevents several LPS-induced inflammatory changes in the hippocampus. Here, we investigated neuroprotective effects of IMT in cortical tissue, and report that the treatment acts as an anti-inflammatory and antioxidative agent, reducing the concentration of TNFalpha and the accumulation of reactive oxygen species. The data couple these changes with an increase in the concentration of the anti-inflammatory cytokine IL-10, and a decrease in activation of the stress-activated protein kinase, c-jun N-terminal kinase. Consistent with these putative protective effects of IMT, we report that the LPS-induced increase in TUNEL staining, which is indicative of cell death, is prevented by IMT.
Nolan
Yvonne
Y
Department of Physiology, Trinity College, Dublin, Ireland.
Campbell
Veronica A
VA
Bolton
Anthony E
AE
Lynch
Marina A
MA
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001804:Blood Transfusion, Autologous; D016923:Cell Death / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology / Q000378:metabolism; D007107:Immune System / Q000187:drug effects* / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D007167:Immunotherapy / Q000379:methods*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008297:Male; D018384:Oxidative Stress / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000276:immunology
D000893:Anti-Inflammatory Agents; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D048031:JNK Mitogen-Activated Protein Kinases