Translocation of Bax in rat hepatocytes cultured with ferric nitrilotriacetate.
Hepatic fibrosis occurs after many years of iron overload in liver. An effective iron deposition model induced by ferric nitrilotriacetate (FeNTA) in cultured rat hepatocytes was assumed. It has been shown that treatment of rat hepatocytes with FeNTA lead to oxidative stress and hepatocyte apoptosis. Hepatocyte apoptosis can promote liver fibrosis. The mechanisms of hepatocyte apoptosis induced by FeNTA have not yet been fully elucidated. The present study demonstrated that FeNTA-induced hepatocyte apoptosis was related to Bax translocation, cytochrome c release, and caspase-3 activation.
Yajun
Zhou
Z
Department of Biochemistry and Molecular Biology, Medical College, Nan Tong University 226001, Jiangsu Province, PR China. zhouyajun64@hotmail.com
Hongshan
Chen
C
Baoxi
Sha
S
Dengbing
Yao
Y
Jianhua
Shi
S
Xinshun
Ge
G
Li
Yin
Y
Yi
Cao
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003124:Colorimetry; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005290:Ferric Compounds / Q000633:toxicity*; D005452:Fluoresceins; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007211:Indoles; D008297:Male; D008954:Models, Biological; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity*; D021381:Protein Transport / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D005290:Ferric Compounds; D005452:Fluoresceins; D007211:Indoles; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C029569:diacetyldichlorofluorescein; C007293:DAPI; D045304:Cytochromes c; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate