Genotyping of Norovirus strains detected in outbreaks between April 2002 and March 2003 in Osaka City, Japan.
Noroviruses (NVs) are the major cause of food- and waterborne nonbacterial gastroenteritis in Japan. Between April 2002 and March 2003, a total of 111 fecal specimens from 40 outbreaks of acute nonbacterial gastroenteritis in Osaka City, Japan were subject to NV detection. Seventy-two samples (64.9%) from 31 outbreaks (77.5%) were NV positive by a real time reverse transcription (RT)-PCR assay. To further determine the genotype of individual NV strains, we sequenced the capsid N-terminal/shell (N/S) domain of some representative strains from each outbreak. The 51 NV strains detected in this study were segregated into 15 genotypes (6 in genogroup I and 9 in genogroup II), and GII/5 genotype NV was a dominant outbreak genotype.
Seto
Yoshiyuki
Y
Department of Virology, Osaka City University Medical School, Osaka, Japan. yoshi3486@med.osaka-cu.ac.jp
Iritani
Nobuhiro
N
Kubo
Hideyuki
H
Kaida
Atsushi
A
Murakami
Tsukasa
T
Haruki
Kosuke
K
Nishio
Osamu
O
Ayata
Minoru
M
Ogura
Hisashi
H
D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction
D036022:Capsid Proteins