title: The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.
abstract: Microtubules have a central role in cell division and cell polarity in eukaryotic cells. The fission yeast is a useful organism for studying microtubule regulation owing to the highly organised nature of its microtubular arrays. To better understand microtubule dynamics and organisation we carried out a screen that identified over 30 genes whose overexpression resulted in microtubule cytoskeleton abnormalities. Here we describe a novel nucleoporin-like protein, Amo1, identified in this screen. Amo1 localises to the nuclear rim in a punctate pattern that does not overlap with nuclear pore complex components. Amo1Delta cells are bent, and they have fewer microtubule bundles that curl around the cell ends. The microtubules in amo1Delta cells have longer dwelling times at the cell tips, and grow in an uncoordinated fashion. Lack of Amo1 also causes a polarity defect. Amo1 is not required for the microtubule loading of several factors affecting microtubule dynamics, and does not seem to be required for nuclear pore function.
authors.affiliation: Cell Cycle Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK. mp3@sanger.ac.uk
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pardo
authors.forename: Paul
authors.initials: P
authors.lastname: Nurse
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D016681:Genome, Fungal; D015698:Genomic Library; D022101:Microtubule-Organizing Center / Q000378:metabolism / Q000648:ultrastructure; D008870:Microtubules / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: C504078:AMo1 protein, S pombe; D018076:DNA, Complementary; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins