title: Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family.
abstract: Yhr049w/FSH1 was recently identified in a combined computational and experimental proteomics analysis for the detection of active serine hydrolases in yeast. This analysis suggested that FSH1 might be a serine-type hydrolase belonging to the broad functional alphabeta-hydrolase superfamily. In order to get insight into the molecular function of this gene, it was targeted in our yeast structural genomics project. The crystal structure of the protein confirms that it contains a Ser/His/Asp catalytic triad that is part of a minimal alpha/beta-hydrolase fold. The architecture of the putative active site and analogies with other protein structures suggest that FSH1 may be an esterase. This finding was further strengthened by the unexpected presence of a compound covalently bound to the catalytic serine in the active site. Apparently, the enzyme was trapped with a reactive compound during the purification process.
authors.affiliation: Institut de Biochimie et de Biophysique Moléculaire et Cellulaire (CNRS-UMR 8619), Université Paris-Sud, Bâtiment 430, 91405 Orsay, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Quevillon-Cheruel
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Marc
authors.initials: M
authors.lastname: Graille
authors.forename: Nadège
authors.initials: N
authors.lastname: Hervouet
authors.forename: Frank
authors.initials: F
authors.lastname: Coste
authors.forename: Hélène
authors.initials: H
authors.lastname: Bénédetti
authors.forename: Charles
authors.initials: C
authors.lastname: Zelwer
authors.forename: Joel
authors.initials: J
authors.lastname: Janin
authors.forename: Herman
authors.initials: H
authors.lastname: Van Tilbeurgh
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry*; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D006867:Hydrolases