title: Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress.
abstract: DNA microarray was used as a tool to investigate genome-wide transcriptional responses of Yersinia pestis to hyperosmotic and high-salinity stress. Hyperosmotic stress specifically upregulated genes responsible for ABC-type transport and the cytoplasmic accumulation of certain polysaccharides, while high-salinity stress induced the transcription of genes encoding partition proteins and several global transcriptional regulators. Genes whose transcription was enhanced by both kinds of stress comprised those encoding osmoprotectant transport systems and a set of virulence determinants. The number of genes downregulated by the two kinds of stress was much lower than that of upregulated genes, suggesting that neither kind of stress severely depresses cellular processes in general. Many differentially regulated genes still exist whose functions remain unknown. Y. pestis recognized high-salinity and hyperosmotic stress as different kinds of environmental stimuli, and different mechanisms enabled acclimation to these two kinds of stress, although Y. pestis still executed common mechanisms to accommodate both types of stress.
authors.affiliation: Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Zongzhong
authors.initials: Z
authors.lastname: Tong
authors.forename: Jingyue
authors.initials: J
authors.lastname: Bao
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Peitang
authors.initials: P
authors.lastname: Huang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic; D014158:Transcription, Genetic / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000502:physiology
chemical_list: D012329:RNA, Bacterial; D012462:Saline Solution, Hypertonic