Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage.
Proliferating cell nuclear antigen (PCNA) is an essential protein for both DNA replication and DNA repair. In the present study using two-hybrid analysis with PCNA from rice, Oryza sativa L. cv. Nipponbare (OsPCNA), we found that OsPCNA interacted with rice DnaJ protein. We have identified DnaJ and designated it as OsDnaJ. OsDnaJ was able to bind to OsPCNA in vitro. Transcripts of OsDnaJ were found to be strongly expressed in the proliferating cells. mRNA of DnaJ was induced by UV and DNA-damaging agents such as H2O2. The expression patterns of OsPCNA were almost the same as OsDnaJ. The relationship between OsPCNA and OsDnaJ is discussed.
Yamamoto
Taichi
T
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Mori
Yoko
Y
Ishibashi
Toyotaka
T
Uchiyama
Yukinobu
Y
Ueda
Tadamasa
T
Ando
Tsuyu
T
Hashimoto
Junji
J
Kimura
Seisuke
S
Sakaguchi
Kengo
K
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004249:DNA Damage / Q000502:physiology*; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D018744:DNA, Plant; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen