Cutting edge: IFN-gamma signaling to macrophages is required for optimal Valpha14i NK T/NK cell cross-talk.
Activated NK T cells are known to rapidly stimulate NK cells and, subsequently, CD8(+) T cells and B cells. In this report, we first demonstrate that the downstream effects induced by alpha-galactosylceramide activated NK T cells on NK cells are mainly dependent on IFN-gamma. We found that NK T cell activation of NK cells requires a functional IFN-gamma signaling in macrophages and dendritic cells but not in B cells, NK cells, or NK T cells. NK T cell activation is dendritic cell-dependent whereas NK T cell activation of NK cells is indirect and in part mediated by macrophages. Interestingly, in this context, macrophage participation in the CD1d Ag presentation of alpha-galactosylceramide to NK T cells is not necessary. These data indicate that NK T cell-dependent activation of macrophages is required for optimal NK T cell-induced stimulation of NK cells.
Wesley
Johnna D
JD
Department of Molecular Microbiology and Immunology and Graduate Program in Pathobiology, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.
Robbins
Scott H
SH
Sidobre
Stéphane
S
Kronenberg
Mitchell
M
Terrizzi
Stephanie
S
Brossay
Laurent
L
D000818:Animals; D017951:Antigen Presentation; D002450:Cell Communication / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology; D005699:Galactosylceramides / Q000276:immunology; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000276:immunology / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008099:Liver / Q000166:cytology; D008213:Lymphocyte Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology
D005699:Galactosylceramides; C493154:alpha-galactosylceramide; D007371:Interferon-gamma