The RET finger protein interacts with the hinge region of SMC3.
The structural maintenance of chromosome 3 protein (SMC3) is a component of the multimeric cohesin complex that holds sister chromatids together and prevents their premature separation during mitosis. By screening a human cDNA library for interacting proteins we have established that the proto-oncogene RET finger protein (RFP) interacts with SMC3. The sites of interaction map to part of the central coiled coil region of RFP and to the C-terminal region of the SMC3 globular hinge domain. SMC3/RFP interaction was confirmed in vivo by co-immunoprecipitation studies and by performing mammalian two-hybrid interaction assays. Cytoimmunolocalization experiments showed that SMC3 and RFP co-localize in the same cell substructures. Overexpression of RFP in NIH3T3 cells significantly increased the fraction of SMC3 recovered in the nucleus supporting the idea that RFP regulates the intracellular distribution of SMC3. These studies identify a novel SMC3-interacting protein that may affect SMC3 availability to complex with its cohesin partners.
Patel
Chirag A
CA
Department of Pathology and Cell Biology, Thomas Jefferson University, 1020 Locust Street, JAH 371, Philadelphia, PA 19107, USA.
Ghiselli
Giancarlo
G
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D020798:Two-Hybrid System Techniques; D000070584:Tripartite Motif Proteins
D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; C000715470:MAS1 protein, human; C492318:SMC3 protein, human; C064419:TRIM27 protein, human; D000070584:Tripartite Motif Proteins