title: [Influence of tangential excision within 24 postburn hours on the local wound inflammatory response in patients with deep partial thickness burn].
abstract: OBJECTIVE
  To investigate the influence of early tangential excision within 24 postburn hours on local wound inflammatory response and tissue injury in patients with deep partial thickness burn.
  
  
  METHODS
  Twelve patients with deep partial thickness burn were enrolled in the study. Tangential excision was performed within 24 postburn hours. The same wound of a patient was divided into three areas for harvesting tissue samples, i.e. pre-operation, post-operation and non-operation areas. For each patient, the biopsies from the pre-operation area were harvested before tangential excision, while those from post-operation and non-operation areas were harvested during 5 to 7 postburn days (PBDs). The contents of IL-8, MPO and MDA in wound tissue were determined by tissue culture and chemical colorimetry, respectively. HE and Mason's staining were employed to assess the degree of necrosis of the wound tissue.
  
  
  RESULTS
  The IL-8, MPO and MDA contents in the local wound tissue after tangential excision were 6.83 +/- 1.85 microg/L, 4.07 +/- 0.87 U/g, and 8.94 +/- 5.66 micromol/g, respectively, which were significantly lower than those from non-operation area (P < 0.01). The inflammatory response in the non-operative wound area was distinct with expansion of necrotic tissue area. In contrast, the local inflammatory response of the wound after tangential excision was ameliorated without the enlargement of necrotic tissue area.
  
  
  CONCLUSIONS
  Tangential excision within 24 postburn hours could be beneficial in ameliorating local inflammatory response, and in preventing progressive deepening of the burn wounds, thus it could accelerate wound healing in patients with deep partial thickness burn.
authors.affiliation: Department of Burns, Ruijin Hospital of Shanghai Second Medical University. Shanghai 200025, P.R. China.
authors.forename: Shu-Liang
authors.initials: SL
authors.lastname: Lu
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Liao
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Yang
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Jin
authors.forename: Ji-Xiang
authors.initials: JX
authors.lastname: Shi
mesh_terms: D000328:Adult; D002056:Burns / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D007249:Inflammation; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D009195:Peroxidase / Q000378:metabolism; D013530:Surgical Wound Infection; D014945:Wound Healing
chemical_list: D016209:Interleukin-8; D008315:Malondialdehyde; D009195:Peroxidase