title: Two novel genes, Gpr113, which encodes a family 2 G-protein-coupled receptor, and Trcg1, are selectively expressed in taste receptor cells.
abstract: To identify genes important for taste receptor cell function, we analyzed the sequences and expression patterns of clones isolated from a mouse taste receptor cell-enriched cDNA library. Here, we report the analyses of two novel genes, Gpr113 and Trcg1. Gpr113 encodes a G-protein-coupled receptor belonging to family 2B, members of which are characterized by having long N-terminal, extracellular domains. The predicted N-terminal extracellular domain of GPR113 contains 696 amino acids with two functional domains, a peptide hormone-binding domain and a G-protein-coupled receptor proteolytic site. Expression analyses indicate that Gpr113 expression is highly restricted to a subset of taste receptor cells. TRCG1 is also selectively expressed in a subset of taste receptor cells. Trcg1 is alternatively spliced and encodes Trcg1 isoforms of 209 and 825 amino acids. BLAST searches of genomic sequences indicate that a putative homolog of Trcg1 resides on human chromosome 15q22.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Rockville, MD 20850, USA.
authors.forename: Nelson D
authors.initials: ND
authors.lastname: LopezJimenez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sainz
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Cavenagh
authors.forename: Mayra A
authors.initials: MA
authors.lastname: Cruz-Ithier
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Blackwood
authors.forename: James F
authors.initials: JF
authors.lastname: Battey
authors.forename: Susan L
authors.initials: SL
authors.lastname: Sullivan
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D013650:Taste Buds / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: C503993:Adgrf3 protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1