A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes.
OBJECTIVE
To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast.


METHODS
Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T(0) (baseline) and T(4d) just before the breakfast + HT administration and at time points 1, 2, 3 and 4 h after T(0). Urines (24-h) were collected from T(0) to T(4d). Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6-24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal.


RESULTS
The major finding was the 46% decrease in the serum TXB(2) production after blood clotting at T(4d). Plasma vitamin A, E, beta-carotene were not changed. Vitamin C tended to increase (P = 0.075). Plasma antioxidant capacity was enhanced at T(0)+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF(2alpha) levels were highly variable and were not affected significantly by HT administration.


CONCLUSIONS
The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes.
Léger
C L
CL
EA Nutrition Humaine et Athérogénèse, Université Montpellier 1, Institut de Biologie, France. leger@univ-montp1.fr
Carbonneau
M A
MA
Michel
F
F
Mas
E
E
Monnier
L
L
Cristol
J P
JP
Descomps
B
B
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000494:pharmacology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000188:drug therapy / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D008875:Middle Aged; D000069463:Olive Oil; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D013929:Thromboxane B2 / Q000097:blood*; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods; D014866:Waste Products
D000975:Antioxidants; D000069463:Olive Oil; D010938:Plant Oils; D014866:Waste Products; C005975:3,4-dihydroxyphenylethanol; C075750:8-epi-prostaglandin F2alpha; D013929:Thromboxane B2; D015237:Dinoprost; D010626:Phenylethyl Alcohol