title: Diversity of carbazole-degrading bacteria having the car gene cluster: isolation of a novel gram-positive carbazole-degrading bacterium.
abstract: Twenty-seven carbazole-utilizing bacterial strains were isolated from environmental samples, and were classified into 14 groups by amplified ribosomal DNA restriction analysis. Southern hybridization analyses showed that 3 and 17 isolates possessed the car gene homologs of Pseudomonas resinovorans CA10 and Sphingomonas sp. strain KA1, respectively. Of the 17 isolates, 2 isolates also have the homolog of the carAa gene of Sphingomonas sp. strain CB3. While the genome of one isolate, a Gram-positive Nocardioides sp. strain IC177, showed no hybridization to any car gene probes, PCR and sequence analyses indicated that strain IC177 had tandemly linked carAa and carC gene homologs whose deduced amino acid sequences showed 51% and 36% identities with those of strain KA1.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Inoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D002227:Carbazoles; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C041514:carbazole; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase