Conversion of proteins from a non-polarized to an apical secretory pattern in MDCK cells.
Previously it was shown that fusion proteins containing the amino terminus of an apical targeted member of the serpin family fused to the corresponding carboxyl terminus of the non-polarized secreted serpin, antithrombin, are secreted mainly to the apical side of MDCK cells. The present study shows that this is neither due to the transfer of an apical sorting signal from the apically expressed proteins, since a sequence of random amino acids acts the same, nor is it due to the deletion of a conserved signal for correct targeting from the non-polarized secreted protein. Our results suggest that the polarity of secretion is determined by conformational sensitive sorting signals.
Vogel
Lotte K
LK
Department of Medical Biochemistry and Genetics, University of Copenhagen, Denmark. vogel@imbg.ku.dk
Larsen
Jakob E
JE
Hansen
Martin
M
Truffer
Renato
R
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D004285:Dogs; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000502:physiology; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D021382:Protein Sorting Signals; D011506:Proteins