title: Parallel nanoliter detection of cancer markers using polymer microchips.
abstract: A general multipurpose microchip technology platform for point-of-care diagnostics has been developed. Real-time nucleic acid sequence-based amplification (NASBA) for detection of artificial human papilloma virus (HPV) 16 sequences and SiHa cell line samples was successfully performed in cyclic olefin copolymer (COC) microchips, incorporating supply channels and parallel reaction channels. Samples were distributed into 10 parallel reaction channels, and signals were simultaneously detected in 80 nl volumes. With a custom-made optical detection unit, the system reached a sensitivity limit of 10(-6) microM for artificial HPV 16 sequences, and 20 cells microl(-1) for the SiHa cell line. This is comparable to the detection limit of conventional readers, and clinical testing of biological samples in polymer microchips using NASBA is therefore possible.
authors.affiliation: NorChip AS, Industriveien 8, 3490 Klokkarstua, Norway. anja.gulliksen@norchip.com
authors.forename: Anja
authors.initials: A
authors.lastname: Gulliksen
authors.forename: Lars Anders
authors.initials: LA
authors.lastname: Solli
authors.forename: Klaus Stefan
authors.initials: KS
authors.lastname: Drese
authors.forename: Olaf
authors.initials: O
authors.lastname: Sörensen
authors.forename: Frank
authors.initials: F
authors.lastname: Karlsen
authors.forename: Henrik
authors.initials: H
authors.lastname: Rogne
authors.forename: Eivind
authors.initials: E
authors.lastname: Hovig
authors.forename: Reidun
authors.initials: R
authors.lastname: Sirevåg
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D021161:Self-Sustained Sequence Replication / Q000379:methods; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D014408:Biomarkers, Tumor; D011108:Polymers; D012367:RNA, Viral