title: In vitro function of platelet concentrates prepared after filtration of whole blood or buffy coat pools.
abstract: METHODS
  Data on the quality of platelet concentrates (PC) produced by the buffy coat method and stored beyond 5 days in plasma are limited. We therefore evaluated the quality of PCs prepared by leucocyte depletion of whole blood (Terumo WBSP, n = 10) or a buffy coat pool (Pall Autostop, n = 10), and stored for 7 days in plasma by assessing platelet parameters and markers of platelet activation.
  
  
  RESULTS
  In both types of PC, levels of glucose decreased during storage but were not totally depleted (> 11 mM on day 7). In contrast, lactate levels increased on storage and was consistently < 20 mM throughout, with pH maintained at > 6.8 in all units. Hypotonic shock response scores were > 47% in all units at day 7. On day 1, markers of platelet activation were significantly higher in WBSP PC, but by day 7 were similar for percentage CD63+ and CD62P + (40%) with levels of platelet microparticles and annexin V binding two-fold higher in WBSP. The expression of CD61 did not alter during storage and the percentage of platelets expressing CD42b was > 88% in all units on day 7. RANTES (Regulated on activation, normal, T-cell expressed and secreted) and TGFbeta released from platelets by day 7 was < 800 ng/ml and 90 ng/ml, respectively. C3a(desarg) increased throughout storage in both types of PC, but without a commensurate increase in the terminal complex SC5b-9 or activation of factor XII.
  
  
  CONCLUSIONS
  Our data indicates that the in vitro characteristics of PCs prepared using these methods is maintained over storage for 7 days in plasma and is not associated with significant deterioration of platelet function. ONE SENTENCE SUMMARY: In vitro function of platelet concentrates prepared by either filtration of whole blood, or pooled buffy coats.
authors.affiliation: Components Development Laboratory, National Blood Service, Brentwood, Essex, UK. Craig.Turner@nbs.nhs.uk
authors.forename: C P
authors.initials: CP
authors.lastname: Turner
authors.forename: J
authors.initials: J
authors.lastname: Sutherland
authors.forename: M
authors.initials: M
authors.lastname: Wadhwa
authors.forename: P
authors.initials: P
authors.lastname: Dilger
authors.forename: R
authors.initials: R
authors.lastname: Cardigan
mesh_terms: D000704:Analysis of Variance; D017304:Annexin A5 / Q000737:chemistry; D015703:Antigens, CD / Q000096:biosynthesis; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019007:P-Selectin / Q000096:biosynthesis; D015539:Platelet Activation; D010976:Platelet Count; D010979:Platelet Function Tests; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D017713:Platelet Transfusion / Q000379:methods; D011485:Protein Binding; D013048:Specimen Handling; D060149:Tetraspanin 30; D013997:Time Factors
chemical_list: D017304:Annexin A5; D015703:Antigens, CD; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30