The shape of cells adhering to sulfonated copolymer surfaces.
We studied the shape of L1210 leukaemia cells adhering in a protein-free medium to sulfonated (styrene/methyl methacrylate) copolymer surfaces of two sulfonic group densities, and thus of differing wettability. The use of our image analysis method and the mathematical procedure [Kowalczynska, H.M. et al, Colloids Surfaces B: Biointerfaces, 30 (2003) 193-206.] allowed us to calculate the values of the so-called shape parameter, which quantitatively determines the three-dimensional cell shape. Here, we show that the values of the shape parameter of the adhering cells and the F-actin concentration, in the region near the cell-substratum interface, depend on the density of sulfonic groups present on the substratum surface.
Kowalczyńska
Hanna M
HM
Department of Biophysics and Biomathematics, Medical Centre for Postgraduate Education, Marymoncka 99, 01-813 Warszawa, Poland. hmkowal@cmkp.edu.pl
Nowak-Wyrzykowska
Małgorzata
M
Inkielman
Marcin
M
Stołowska
Liliana
L
Marciniak
Ewa
E
D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008768:Methylmethacrylates; D011108:Polymers; D013343:Styrenes; D014407:Tumor Cells, Cultured
D000199:Actins; D008768:Methylmethacrylates; D011108:Polymers; D013343:Styrenes