title: Temporal profiling of gene expression during neurogenesis and remodeling in the olfactory epithelium at short intervals after target ablation.
abstract: Neurogenesis in the olfactory epithelium (OE) is induced by olfactory bulbectomy (OBX), which effectively axotomizes olfactory sensory neurons (OSNs) and removes their synaptic targets, resulting in apoptosis. We used Affymetrix high-density oligonucleotide arrays to investigate changes in gene expression during initiation of signaling in pathways that regulate apoptosis and neurogenesis in the murine OE at 2, 8, 16, and 48 hr after bilateral OBX compared to that in sham-operated controls. We focused on regulation of a defined set of genes associated with apoptosis, stem/progenitor cell regulation, and cell cycle progression because of the activation of these processes in OE degeneration and remodeling after OBX. After data scrubbing and categorical analysis, one-way analysis of variance identified 72 genes (4.9% of the present known genes) as being regulated significantly (P < 0.05) at one or more points; 50 were defined as regulated differentially with the false discovery rate at 10%. Significant changes in gene expression occurred in all categories as early as 2 hr post-OBX, with the greatest number of differentially regulated genes at 16 and 48 hr. Hierarchical cluster analysis and correlation coefficients were used to identify similarities in patterns of gene expression changes within and across categories. Validation was carried out with SuperArray macroarrays and real-time RT-PCR. Our results confirmed the participation of many genes in known signaling pathways and identified changes in the expression of 42 genes not identified previously as participating in apoptosis and neurogenesis in the OE. Additionally, our analyses indicated the early involvement of genes regulating cytoskeletal reorganization and angiogenesis in the response to OBX. These studies are an important first step in defining early time-dependent changes in gene expression after target ablation that lead to neurogenesis in the olfactory sensory epithelium.
authors.affiliation: Department of Physiology, University of Kentucky, Lexington, 40536-0230, USA. tgetche@uky.edu
authors.forename: Thomas V
authors.initials: TV
authors.lastname: Getchell
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Radhika A
authors.initials: RA
authors.lastname: Vaishnav
authors.forename: Kevin
authors.initials: K
authors.lastname: Kwong
authors.forename: Arnold J
authors.initials: AJ
authors.lastname: Stromberg
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Getchell
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D003714:Denervation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D005810:Multigene Family / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D009830:Olfactory Bulb / Q000293:injuries*; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism*; D061219:Olfactory Nerve Injuries; D020411:Oligonucleotide Array Sequence Analysis; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; D009419:Nerve Tissue Proteins