Immunohistochemical study of p47Phox and gp91Phox distributions in rat brain.
NADPH oxidase is multi-component enzyme, which comprises the cytosolic proteins p40Phox, p47Phox, and p67Phox and the two membrane proteins, gp91Phox and p22Phox, and which is well characterized in phagocytic cells. NADPH oxidase is a primary source of reactive oxygen species (ROS), and recent studies indicate that free radicals and ROS might be causative factors of several brain degenerative diseases and dysfunctions. However, though previous studies have shown the presence of NADPH oxidase subunits in cell culture and mouse brain, they have not provided detailed high power resolution data. Therefore, we investigated the distributions of the p47Phox and gp91Phox subunits in rat brain using immunohistochemical approach. Cortex, hippocampus, and Purkinje cells of cerebellum were prominently stained by p47Phox and gp91Phox antibodies. As compared with the distributions of p47Phox, gp91Phox in mouse, some differences in the rat brain were observed in the hippocampus, thalamus, amygdala, reticular nucleus, and basal ganglia. Additionally, at the cellular level, most p47Phox immunoreactivity was largely confined to cell bodies and proximal portions of the dendritic tree. Taken together, the widespread observed distributions of p47Phox and gp91Phox subunits indicate that they are probably needed to maintain normal brain function.
Kim
Myeung Ju
MJ
Department of Anatomy, Cheju National University College of Medicine, Ara 1 Dong, Jeju, Jeju-do 690-756, South Korea.
Shin
Kyung-Sue
KS
Chung
Young-Bae
YB
Jung
Kee Wook
KW
Cha
Choong Ik
CI
Shin
Dong Hoon
DH
D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000096:biosynthesis; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000032:analysis* / Q000096:biosynthesis; D010750:Phosphoproteins / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D008562:Membrane Glycoproteins; D010750:Phosphoproteins; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1