Pathology of the future: molecular profiling for targeted therapy.
Recent evidence suggests that each patient's cancer has a unique subset of molecular pathogenetic derangements. These derangements may both genetic and proteomic alterations. Genomic and proteomic research tools enable genome-wide assessment of gene expression as well as kinase driven cell signaling events. These tools are illuminating the molecular derangements of individual tumors, even if these tumors have similar morphological characteristics. A combination of laser capture microdissection with multiplexed phosphoproteomic analysis using reverse phase protein microarray technology is being used to identify protein molecular signatures of individual tumors. The in vivo state of multiple kinase driven signal pathways may be evaluated by reverse phase protein microarray with a panel of specific antibodies developed based upon our knowledge of biological processes. Molecular profiling of individual patient's tumors is currently being evaluated in clinical trials at the National Institutes of Health, National Cancer Institute for monitoring Epidermal Growth Factor (EGF) cell signaling events for patients with breast and ovarian cancer.
Espina
Virginia
V
National Cancer Institute, Laboratory of Pathology, Bethesda, Maryland, USA.
Geho
David
D
Mehta
Arpita I
AI
Petricoin
Emanuel F
EF
Liotta
Lance A
LA
Rosenblatt
Kevin P
KP
D005544:Forecasting; D006801:Humans; D007834:Lasers; D042282:Microdissection; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010336:Pathology / Q000639:trends*; D040901:Proteomics / Q000639:trends*; D020133:Reverse Transcriptase Polymerase Chain Reaction