title: Membranes for biohybrid liver support: the behaviour of C3A hepatoblastoma cells is dependent on the composition of acrylonitrile copolymers.
abstract: Co-polymers based on acrylonitrile, N-vinylpyrrolidone, aminoethylmethacrylate and sodium methallylsulfonate were used to prepare flat membranes by phase inversion. The surface properties of membranes were characterised by water contact angle measurements, atomic force microscopy and X-ray photoelectron spectroscopy (XPS). Membrane permeability was estimated by porosity measurements with water as test liquid. Human C3A hepatoblastoma cells were plated on these materials. Cell-material interaction was characterised by overall cell morphology, formation of focal adhesion contacts and intercellular junctions. Furthermore, cell proliferation was measured and compared with the functional activity of cells as indicated by 7-ethoxycoumarin-O-deethylation. More hydrophilic materials reduced spreading of cells, formation of focal adhesion and subsequent proliferation while homotypic cell adhesion was facilitated in correlation with stronger expressions of intercellular junctions and improved functional activity. In contrast, membranes with stronger adhesivity enhanced cell proliferation but reduced the functional activity of cells. It was concluded that the co-polymerisation of acrylonitrile with hydrophilic co-monomers, such as N-vinylpyrrolidone, could be used to tailor membrane materials for the application in biohybrid liver support systems.
authors.affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Str. Acad. G. Bonchev, bl. 21, BG-1113 Sofia, Bulgaria.
authors.forename: N
authors.initials: N
authors.lastname: Krasteva
authors.forename: B
authors.initials: B
authors.lastname: Seifert
authors.forename: M
authors.initials: M
authors.lastname: Hopp
authors.forename: G
authors.initials: G
authors.lastname: Malsch
authors.forename: W
authors.initials: W
authors.lastname: Albrecht
authors.forename: G
authors.initials: G
authors.lastname: Altankov
authors.forename: Th
authors.initials: T
authors.lastname: Groth
mesh_terms: D000181:Acrylonitrile / Q000737:chemistry*; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D021522:Bioartificial Organs; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D065702:Cytochrome P-450 CYP2B6; D018197:Hepatoblastoma / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D013057:Spectrum Analysis; D016596:Vinculin / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D014867:Water; D016596:Vinculin; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating; D000181:Acrylonitrile