Molecularly imprinted TiO2 thin film by liquid phase deposition for the determination of L-glutamic acid.
For the first time, the feasibility of a molecularly imprinted liquid phase deposition (LPD) thin film has been demonstrated. Thin films of titanium oxide imprinted with L-glutamic acid were prepared by the LPD method on a gold-coated quartz crystal microbalance. The imprinted molecule could be removed upon treatment with immersion in deionized water. A sensor was developed on the basis of this method and showed good sensitivity, selectivity, and reproducibility to the template molecule. An equation was deduced to characterize the interaction between molecularly imprinted films and the template by virtue of Scatchard analysis. X-ray photoelectron spectroscopy was introduced to show the evidence for the molecular imprinting phenomenon. The linear relationship between the frequency shifts and the concentration of analyte in the range of 10-200 microM was obtained. LPD proves to be a powerful method for imprinting titanium oxide thin films.
Feng
Liang
L
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Liu
Yongjun
Y
Hu
Jiming
J
D015374:Biosensing Techniques / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D018698:Glutamic Acid / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D011791:Quartz / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D011791:Quartz; C009495:titanium dioxide; D018698:Glutamic Acid; D006046:Gold; D014025:Titanium