title: A fluorimetric assay for the spontaneous release of an N7-alkylguanine residue from duplex DNA.
abstract: A fluorimetric assay for monitoring depurination of the N7-alkylguanine adduct derived from the anticancer natural product leinamycin is described. This general approach could potentially provide the foundation for a high throughput assay that detects DNA-alkylating agents or a convenient continuous fluorimetric assay for base excision repair enzymes.
authors.affiliation: Department of Chemistry, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Shipova
authors.forename: Kent S
authors.initials: KS
authors.lastname: Gates
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000737:chemistry / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D007769:Lactams / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013871:Thiones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D007769:Lactams; D018942:Macrolides; D009692:Nucleic Acid Heteroduplexes; D013844:Thiazoles; D013871:Thiones; C059000:leinamycin; D006147:Guanine; D004247:DNA