title: Transplantation of neonatal porcine islets and sertoli cells into nonimmunosuppressed nonhuman primates.
abstract: A Mexican group reported transplantation of cocultured neonatal porcine islets and Sertoli cells resulting in insulin independence in nonimmunosuppressed type 1 diabetes patients. We have transplanted similar islets alone (naked islets) or cocultured islets with sertoli cells (islet/sertoli cells) into an omental site and other locations of seven nondiabetic, nonimmunosuppressed, nonhuman primates. Porcine endogenous retrovirus was not detected in recipient blood 8 weeks after porcine islet grafts, and porcine C-peptide was detected at a very low level in all animals. Histology examination failed to demonstrate obviously recognizable islets, but in the animals transplanted with islet/Sertoli cells at the omentum site, there were some surviving glucagons, pan-cytokeratin, and inhibin stained cells at 8 weeks.
authors.affiliation: Department of Surgery, National University of Singapore, Singapore.
authors.forename: J R
authors.initials: JR
authors.lastname: Isaac
authors.forename: S
authors.initials: S
authors.lastname: Skinner
authors.forename: R
authors.initials: R
authors.lastname: Elliot
authors.forename: M
authors.initials: M
authors.lastname: Salto-Tellez
authors.forename: O
authors.initials: O
authors.lastname: Garkavenko
authors.forename: A
authors.initials: A
authors.lastname: Khoo
authors.forename: K O
authors.initials: KO
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Calne
authors.forename: D Z
authors.initials: DZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006085:Graft Survival; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D008251:Macaca; D008297:Male; D012708:Sertoli Cells / Q000637:transplantation*; D013552:Swine; D014183:Transplantation, Heterologous