title: Effect of a fermented ginseng extract, BST204, on the expression of cyclooxygenase-2 in murine macrophages.
abstract: This paper investigates how BST204, a fermented ginseng extract, affects the expression and mechanism of cyclooxygenase-2 (COX-2). BST204 was prepared by incubating crude ginseng extract with ginsenoside-beta-glucosidase. Unexpectedly, BST204 had no effect on the level of COX-2 protein in unstimulated RAW 264.7 cells, and it suppressed the level of COX-2 protein and PGE(2) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. It did not show any suppressive effect, though, on the COX-2 mRNA level. To investigate the suppressive mechanism of COX-2 protein, the activating phosphorylation of p70 S6 kinase and 4E-BP1, which are important for translation, were measured. The phosphorylation of p70 S6 kinase, not 4E-BP1, was increased by LPS in a time-dependent manner, and was inhibited by BST204 in a dose-dependent manner. The expression of COX-2 protein, however, was partially suppressed by rapamycin, an upstream inhibitor of p70 S6 kinase. Therefore, this paper suggests that the suppression of COX-2 protein by BST204 was partially correlated with the inhibition of p70 S6 kinase activation.
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, South Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Seo
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Nam Wook
authors.initials: NW
authors.lastname: Kim
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Chang
authors.forename: Na Young
authors.initials: NY
authors.lastname: Ko
authors.forename: Young Hyo
authors.initials: YH
authors.lastname: Yoo
authors.forename: Jie Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Dong Wan
authors.initials: DW
authors.lastname: Seo
authors.forename: Jeung Whan
authors.initials: JW
authors.lastname: Han
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D051546:Cyclooxygenase 2; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D005285:Fermentation; D015870:Gene Expression / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology*; D051379:Mice; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D036145:Ginsenosides; D010936:Plant Extracts; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D020123:Sirolimus