[Changes in the distribution of mitochondria in mouse embryos blocked at the two-cell stage].
Changes in the distribution of mitochondria in the two-cell mouse embryos preceding the developmental arrest in vitro, caused by a genetically determined "two-cell block in vitro" or genisteine treatment, were examined vitally using the mitochondrial-specific probe rhodamine 123 and conventional fluorescence microscopy. In the former case, serious disturbances in the localization of mitochondria appeared already from the middle of two-cell stage, long before the time corresponding to the 2nd cleavage division. Comparison of the behavior of mitochondria in the embryos successfully developing between the one- and two-cell stages and that in the embryos that ceased to cleave suggests that the developmental arrest was accompanied by aggregation of the mitochondria into clusters. There are many such clusters unlike in the cytoplasm of normally developing embryos. Intracellular localization of clusters observed in the genisteine-treated embryos differed radically from that observed in the embryos blocked in vitro at the two-cell stage.
Bogoliubova
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NA
D000818:Animals; D001757:Blastomeres / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000166:cytology* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D019833:Genistein / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000648:ultrastructure*; D020112:Rhodamine 123
D005456:Fluorescent Dyes; D020112:Rhodamine 123; D019833:Genistein