Quantitative microarray-based DNA-DNA hybridization assay for measuring genetic distances among bacterial species and its application to the identification of family Enterobacteriaceae.
Quantitative DNA-DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3-labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis. These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA-DNA hybridization for measuring chromosome relatedness among bacterial species.
Amano
Makoto
M
Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd., Amagasaki, Hyogo, Japan.
Ohkusu
Kiyofumi
K
Kusaba
Koji
K
Ikeda
Hironori
H
Nagasawa
Zenzo
Z
Aoki
Yosuke
Y
Kawamura
Yoshiaki
Y
Kobatake
Shinzo
S
Tanaka
Takumi
T
Matsuura
Shuuji
S
Ezaki
Takayuki
T
D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics*; D004925:Escherichia / Q000145:classification / Q000235:genetics; D007709:Klebsiella / Q000145:classification / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics; D012705:Serratia / Q000145:classification / Q000235:genetics; D012760:Shigella / Q000145:classification / Q000235:genetics; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
D004269:DNA, Bacterial