title: Increased activity of lysosomal enzymes in the peritoneal fluid of patients with gynecologic cancers and pelvic inflammatory disease.
abstract: OBJECTIVE
  To investigate whether the activity of lysosomal enzymes is increased in the peritoneal fluid of patients with gynecologic cancers compared to activity in the peritoneal fluid from normal subjects and those with pelvic inflammatory disease, and fluid from benign ovarian cysts.
  
  
  METHODS
  beta-glucuronidase, beta-galactosidase, and alpha-mannosidase activity was measured in the peritoneal fluid from patients with gynecologic cancer, pelvic inflammatory disease, and normal subjects, and fluid from benign ovarian cysts.
  
  
  RESULTS
  The mean+/-SD of beta-glucuronidase, beta-galactosidase, and alpha-mannosidase activity in the gynecologic cancers was 120+/-50 nmol, 203+/-86 nmol, and 240+/-119 nmol 4-methylumbelliferone/ml/h, respectively; in the normal control subjects it was 22+/-9 nmol, 46+/-10 nmol, and 80+/-23 nmol, respectively (P=0.00003, 0.0001, and 0.0001, respectively). The activity was increased even in cases without malignant cells in the peritoneal fluid. In pelvic inflammatory disease it was 148+/-82 nmol, 278+/-112 nmol, and 291+/-140 nmol, respectively. The activity in the fluid of the ovarian cysts was similar to that of the normal peritoneal fluid. There was a significant positive correlation between enzyme activity and stage of cancer, that was stronger for beta-glucuronidase (r=0.889, P=0.003).
  
  
  CONCLUSIONS
  The increased lysosomal enzyme activity in gynecologic cancers, without overlapping between patients and normal subjects or benign ovarian cyst fluid, indicates that such measurements might be applied for diagnostic purposes.
authors.affiliation: Department of Pediatrics, University of Patras Medical School, Rion, 26504, Patras, Greece. nic-bera@med.upatras.gr
authors.forename: N G
authors.initials: NG
authors.lastname: Beratis
authors.forename: A
authors.initials: A
authors.lastname: Kaperonis
authors.forename: M I
authors.initials: MI
authors.lastname: Eliopoulou
authors.forename: G
authors.initials: G
authors.lastname: Kourounis
authors.forename: V A
authors.initials: VA
authors.lastname: Tzingounis
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D018262:Adenocarcinoma, Clear Cell / Q000201:enzymology; D002288:Adenocarcinoma, Mucinous / Q000201:enzymology; D001202:Ascitic Fluid / Q000201:enzymology*; D016022:Case-Control Studies; D018284:Cystadenocarcinoma, Serous / Q000201:enzymology; D016889:Endometrial Neoplasms / Q000201:enzymology; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D009369:Neoplasms / Q000201:enzymology; D010048:Ovarian Cysts / Q000201:enzymology; D010051:Ovarian Neoplasms / Q000201:enzymology*; D000292:Pelvic Inflammatory Disease / Q000201:enzymology*; D043323:alpha-Mannosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D006923:Hymecromone; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D005966:Glucuronidase