title: Expression and regulation of adrenomedullin in renal glomerular podocytes.
abstract: Adrenomedullin (AM) is postulated to exert organ-protective effects. It is expressed in the renal glomeruli, but its roles in the glomerular podocytes have been poorly elucidated. In the present study, we investigated the expression and regulation of AM in recently established conditionally immortalized mouse podocyte cell line in vitro and podocyte injury model in vivo. The cultured differentiated podocytes expressed AM mRNA and secreted measurable amount of AM. AM secretion from the podocytes was increased by H(2)O(2), hypoxia, puromycin aminonucleoside (PAN), albumin overload, and TNF-alpha. Real-time RT-PCR analysis revealed that AM mRNA expression in the podocytes was enhanced by PAN and TNF-alpha, both of which were suppressed by mitochondrial antioxidants. Furthermore, AM expression was upregulated in the glomerular podocytes of PAN nephrosis rats. These results indicated that AM expression in the podocytes was upregulated by stimuli or condition relevant to podocyte injury, suggesting its potential role in podocyte pathophysiology.
authors.affiliation: Department of Nephrology and Endocrinology, University of Tokyo Graduate School of Medicine, Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Hino
authors.forename: Miki
authors.initials: M
authors.lastname: Nagase
authors.forename: Shinya
authors.initials: S
authors.lastname: Kaname
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shibata
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagase
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Oba
authors.forename: Masako
authors.initials: M
authors.lastname: Funaki
authors.forename: Naoto
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawachi
authors.forename: Peter
authors.initials: P
authors.lastname: Mundel
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fujita
mesh_terms: D053607:Adrenomedullin; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000235:genetics*; D011692:Puromycin Aminonucleoside / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D010455:Peptides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D053607:Adrenomedullin; D011692:Puromycin Aminonucleoside