title: [Purification of human serum apolipoprotein A I and A II by middle-pressure liquid chromatography].
abstract: OBJECTIVE
  This study is made for the purification of human HDL apolipoproteins by middle-pressure liquid chromatography (MPLC).
  
  
  METHODS
  Human HDL was isolated by one step density ultracentrifugation. Delipided human HDL was separated by Sephacryl S-200 molecular sieve chromatography. Sephadex G-75 molecular sieve chromatography was used to separate apoA I and apoA II.
  
  
  RESULTS
  Purified apoA I and apoA II were obtained and SDS-PAGE and immunodiffusion test indicated that the proteins are the same as those theoretically predicted.
  
  
  CONCLUSIONS
  We have established a purification procedure for human HDL apolipoproteins with high efficiency and simplicity by MPLC. It could serve as a base for preclinical and clinical trials of HDL apolipoproteins.
authors.affiliation: Apolipoprotein Research Unit, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Xin
authors.forename: Bing-wen
authors.initials: BW
authors.lastname: Liu
authors.forename: Zu-hui
authors.initials: ZH
authors.lastname: Zhang
mesh_terms: D016632:Apolipoprotein A-I / Q000032:analysis / Q000302:isolation & purification*; D016633:Apolipoprotein A-II / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008075:Lipoproteins, HDL / Q000302:isolation & purification
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D008075:Lipoproteins, HDL