title: Glutathione S-transferase P1 genotype and prognosis in Hodgkin's lymphoma.
abstract: OBJECTIVE
  Glutathione S-transferase P1 (GSTP1) is a member of the GST enzyme superfamily that is important for the detoxification of several cytotoxic drugs and their by-products. A single nucleotide polymorphism results in the substitution of isoleucine (Ile) to valine (Val) at codon 105, causing a metabolically less active variant of the enzyme. We assessed the impact of the GSTP1 codon 105 genotype on treatment outcome in patients with Hodgkin's lymphoma.
  
  
  METHODS
  The Ile(105)Val polymorphism in the GSTP1 gene was analyzed using a PCR-RFLP technique. Ninety-seven patients with Hodgkin's lymphoma were included and associations with patient characteristics and treatment outcome were analyzed.
  
  
  RESULTS
  The GSTP1 Ile(105)Val polymorphism was associated in a dose-dependent fashion with an improved failure-free survival in patients with Hodgkin's lymphoma (P = 0.02). The probability of 5-year survival for patients homozygous for the (105)Val/(105)Val GSTP1 genotype was 100%, for heterozygous patients 74% (95% confidence interval, 56-85), and for patients homozygous for the (105)Ile/(105)Ile genotype 43% (95% confidence interval, 23-61). The Cox multivariate analysis showed that GSTP1 codon 105 genotype was an independent prognostic factor.
  
  
  CONCLUSIONS
  The GSTP1 genotype predicts clinical outcome in patients with Hodgkin's lymphoma.
authors.affiliation: Istituto di Ematologia, Universita' Cattolica S. Cuore, Largo A. Gemelli 1, 00168 Rome, Italy. stefanhohaus@hotmail.com
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohaus
authors.forename: Annalisa
authors.initials: A
authors.lastname: Di Ruscio
authors.forename: Annalaura
authors.initials: A
authors.lastname: Di Febo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Massini
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Alo'
authors.forename: Francesco
authors.initials: F
authors.lastname: Guidi
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mansueto
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Voso
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Leone
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018572:Disease-Free Survival; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics*; D006689:Hodgkin Disease / Q000201:enzymology* / Q000235:genetics* / Q000628:therapy; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011379:Prognosis; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase