title: Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae.
abstract: A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated both metabolically and hierarchically. The method is expounded in terms of its implications for diverse types of regulation. It is also fine-tuned for cases where isoenzymes catalyze the flux through a single metabolic step.
authors.affiliation: Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, The Netherlands.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rossell
authors.forename: Coen C
authors.initials: CC
authors.lastname: van der Weijden
authors.forename: Arthur L
authors.initials: AL
authors.lastname: Kruckeberg
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bakker
authors.forename: Hans V
authors.initials: HV
authors.lastname: Westerhoff
mesh_terms: D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D023281:Genomics; D005947:Glucose / Q000378:metabolism*; D018869:Heat-Shock Response; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose; D009584:Nitrogen