Expressed sequence tags from Diabrotica virgifera virgifera midgut identify a coleopteran cadherin and a diversity of cathepsins.
The Western corn rootworm is the major pest of corn in the USA and has recently become the target for insect-resistant transgenic crops. Transgenic crops have switched the focus for identifying insecticide targets from the insect nervous system to the midgut. Here we describe a collection of 691 sequences from the Western corn rootworm midgut, 27% of which predict proteins with no matches in current databases. Of the remaining sequences, most predict proteins with either catalytic (62%) or binding (19%) functions, as expected for proteins expressed in the insect midgut. The utility of this approach for the identification of targets for novel toxins is demonstrated by analysis of the first coleopteran cadherin gene, a putative Bt receptor, and a large class of cysteine-proteases, the cathepsins.
Siegfried
B D
BD
Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, USA. bsiegfried1@unl.edu
Waterfield
N
N
ffrench-Constant
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D002403:Cathepsins / Q000235:genetics*; D016000:Cluster Analysis; D001517:Coleoptera / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D020224:Expressed Sequence Tags; D041981:Gastrointestinal Tract / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D015820:Cadherins; D002403:Cathepsins