title: 19F NMR measurements of NO production in hypertensive ISIAH and OXYS rats.
abstract: Recently we demonstrated the principal possibility of application of 19F NMR spin-trapping technique for in vivo *NO detection [Free Radic. Biol. Med. 36 (2004) 248]. In the present study, we employed this method to elucidate the significance of *NO availability in animal models of hypertension. In vivo *NO-induced conversion of the hydroxylamine of the fluorinated nitronyl nitroxide (HNN) to the hydroxylamine of the iminonitroxide (HIN) in hypertensive ISIAH and OXYS rat strains and normotensive Wistar rat strain was measured. Significantly lower HIN/HNN ratios were measured in the blood of the hypertensive rats. The NMR data were found to positively correlate with the levels of nitrite/nitrate evaluated by Griess method and negatively correlate with the blood pressure. In comparison with other traditionally used methods 19F NMR spectroscopy allows in vivo evaluation of *NO production and provides the basis for in vivo *NO imaging.
authors.affiliation: Institute of Chemical Kinetics and Combustion, Novosibirsk 630090, Russia. bobko@kinetics.nsc.ru
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Bobko
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Sergeeva
authors.forename: Elena G
authors.initials: EG
authors.lastname: Bagryanskaya
authors.forename: Arkadii L
authors.initials: AL
authors.lastname: Markel
authors.forename: Valery V
authors.initials: VV
authors.lastname: Khramtsov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Reznikov
authors.forename: Nataljya G
authors.initials: NG
authors.lastname: Kolosova
mesh_terms: D000818:Animals; D005461:Fluorine; D006973:Hypertension / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats
chemical_list: D005461:Fluorine; D009569:Nitric Oxide