Phosphorylcholine is favorable for antibody production from hybridoma cells.
Growth of antibody-secreting hybridomas requires special conditions such as serum-free defined media containing growth factors and vitamins. However, the surface on which these cells can proliferate has been shown to play an important role. Phosphorylcholine (PC)-based polymers are zwitterionic compounds with nonbiofouling properties. These polymers are characterized by having reduced protein absorption properties. Our aim was to determine whether well-established hybridoma cell lines were able to proliferate and produce measurable amounts of monoclonal antibodies when grown on PC-polymer-coated surfaces. Comparative experiments using four well-known hybridoma cell lines (PAb421, PAb246, PAb1801 which recognize p53, and PAb280 which recognizes SV40 small t antigen) grown on PC-polymer-coated, uncoated, and two commercially available tissue culture plates showed that PC-polymer-coated plates were more efficient than uncoated plates in sustaining cell growth and monoclonal antibody production/secretion as defined by growth assays and ELISA. Also, results demonstrated that PC-polymer-coated plates were able to perform better than commercially available plates. These observations suggest that PC polymers could be used as an alternative, efficient surface coating to grow hybridoma cell lines and allow detectable antibody secretion.
Montano
Ximena
X
Department of Biological Sciences, Imperial College, London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom. x.montano@imperial.ac.uk
Lewis
Andrew L
AL
Leppard
Simon W
SW
Lichtenstein
Conrad
C
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology / Q000502:physiology; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000502:physiology*; D008422:Materials Testing; D010767:Phosphorylcholine / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D010767:Phosphorylcholine