title: Expression and distribution of CB1 cannabinoid receptors in the central nervous system of the African cichlid fish Pelvicachromis pulcher.
abstract: Neuroanatomical investigation of the cannabinoid system in a lower vertebrate group such as teleost fishes might improve our understanding of the physiological role of such a signaling system. In the present study, the expression of a CB1 cannabinoid receptor has been demonstrated in the CNS of a teleost fish, the cichlid Pelvicachromis pulcher. Moreover, CB1-like immunoreactivity has been analyzed by using a purified antibody against the CB1 receptor amino-terminus. Immunostained neurons and varicosities were found through the telencephalon as well as in the preoptic area and lateral infundibular lobes of the hypothalamus. Stained cells were observed in the pituitary gland. Several cell bodies and nerve terminals containing an intense CB1-like immunoreactivity were found in the pretectal central nucleus and posterior tuberculum, both lying in a transitional region between diencephalon and mesencephalon. In the brainstem, the CB1 immunopositivity was more restricted than in the prosencephalon, with the exception of some large, intensely immunopositive nerve cells within the dorsal mesencephalic tegmentum, possibly motor neurons of the third cranial nerve. In the cerebellum, among a majority of immunonegative granule cells, a subset of them was immunostained. Some positive Purkinje cells were also observed. In the spinal cord, ventral gray matter, several alpha-motoneurons were stained. Similarities to and discrepancies from the CB1 receptor distributions in other vertebrate CNS are discussed, paying particular attention to the abundant CB1 immunoreactivity observed in the area encompassing the pretectum and glomerular nucleus, which is characterized by a peculiar differentiation in bony fishes.
authors.affiliation: Dipartimento di Biologia Animale e dell'Uomo, Università degli Studi di Torino, 10123 Torino, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Cottone
authors.forename: Sarah
authors.initials: S
authors.lastname: Forno
authors.forename: Ezio
authors.initials: E
authors.lastname: Campantico
authors.forename: Alda
authors.initials: A
authors.lastname: Guastalla
authors.forename: Laura
authors.initials: L
authors.lastname: Viltono
authors.forename: Ken
authors.initials: K
authors.lastname: Mackie
authors.forename: Maria Fosca
authors.initials: MF
authors.lastname: Franzoni
mesh_terms: D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D002490:Central Nervous System / Q000737:chemistry / Q000378:metabolism*; D023681:Cichlids / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D043884:Receptor, Cannabinoid, CB1 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D043884:Receptor, Cannabinoid, CB1