Chemiluminescence of enterococci isolates from freshwater.
All Enterococcus spp., isolated from environmental water samples (n=81), emitted a high chemiluminescence signal in the presence of luminol (10(-2) M). Kinetic studies of chemiluminescence show a close correlation between chemiluminescence and growth curves during the exponential phase, with a maximum chemiluminescence reached just before bacterial growth entered in the stationary phase. On the other hand, genera closely related to Enterococcus such as Streptococcus or Lactococcus produced a very weak chemiluminescent signal. Chemiluminescence of enterococci could therefore offer a rapid test, in aiding the identification of the genus Enterococcus and in the survey of the microbiological quality of water supplies.
Andre
Philippe
P
U.F.R. des Sciences Pharmaceutiques, UE-3429, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France. andre@pharma.u-strasbg.fr
Metzger
Catherine
C
Petey
Sophie
S
Muller
Daniel
D
Vidon
Dominique J-M
DJ
D015373:Bacterial Typing Techniques; D003470:Culture Media; D016983:Enterococcus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D005618:Fresh Water / Q000382:microbiology*; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D013045:Species Specificity
D003470:Culture Media; D008165:Luminol