title: Distinct patterns of angiogenesis in oral and skin wounds.
abstract: Clinical observation suggests that oral mucosal wounds heal faster than skin; however, little is known about the site-specific differences. Since fetal skin wounds heal rapidly, but are less vascular than adult wounds, we hypothesized that less robust wound angiogenesis might be observed in healing oral mucosa. This study investigated angiogenesis in equivalent-size oral and skin murine wounds. Change in wound bed vascularity was significantly lower in oral wounds than in skin. Also, vascular endothelial growth factor (VEGF) levels were less in oral than cutaneous wounds. Because keratinocytes are a prominent source of VEGF in wounds, we compared VEGF production by oral and epidermal keratinocytes in vitro. Significantly higher levels of VEGF protein and mRNA were observed in epidermal keratinocytes than in oral keratinocytes after 18 hrs of hypoxia. This study demonstrates distinct angiogenesis patterns in oral and skin wounds and intrinsic site-specific differences in VEGF production by keratinocytes.
authors.affiliation: Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, IL 60153, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Szpaderska
authors.forename: C G
authors.initials: CG
authors.lastname: Walsh
authors.forename: M J
authors.initials: MJ
authors.lastname: Steinberg
authors.forename: L A
authors.initials: LA
authors.lastname: DiPietro
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000098:blood supply* / Q000293:injuries* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000098:blood supply* / Q000293:injuries* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2