title: Deubiquitinating enzyme USP36 contains the PEST motif and is polyubiquitinated.
abstract: The ubiquitin-mediated protein degradation pathway has been emphasized for the regulation of numerous cellular mechanisms and the significance of deubiquitination, mediated by deubiquitinating (DUB) enzymes, has been emerging as an essential regulatory step to control these cellular mechanisms. Previously, we demonstrated a human DUB enzyme, HeLa DUB-1, expressed in human ovarian cancer cells. Here, we report human USP36, which has the extension of the C-terminal region of HeLa DUB-1 and has conserved amino acid domains as previously shown in other DUBs. Human USP36, encoding a DUB enzyme, was isolated from ovarian cancer cells using RT-PCR and characterized. We identified DUB enzyme activity of USP36 by analyzing its capability to cleave the ubiquitin. Interestingly, structural and immunoprecipitation analyses revealed for the first time that USP36 contains the PEST motif and is polyubiquitinated.
authors.affiliation: Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul 135-081, Republic of Korea.
authors.forename: Myung-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Yu-Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Minu
authors.initials: M
authors.lastname: Seong
authors.forename: Joong-Kook
authors.initials: JK
authors.lastname: Choi
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D025801:Ubiquitin / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; C501066:USP36 protein, human; D025801:Ubiquitin; D043222:Ubiquitin Thiolesterase; D003546:Cysteine Endopeptidases