Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3.
BACKGROUND
The search for the dengue virus receptor has generated many candidates often identified only by molecular mass. The wide host range of the viruses in vitro combined with multiple approaches to identifying the receptor(s) has led to the notion that many receptors or attachment proteins may be involved and that the different dengue virus serotypes may utilize different receptors on the same cells as well as on different cell types.


RESULTS
In this study we used sequential extraction of PS Clone D cell monolayers with the detergent beta-octylglucopyranoside followed by sodium deoxycholate to prepare a cell membrane-rich fraction. We then used 2 dimensional (2D) gel electrophoresis to separate the membrane proteins and applied a modified virus overlay protein binding assay (VOPBA) to show that dengue virus serotypes 1, 2 and 3 all interact with the 37 kDa/67 kDa laminin receptor (LAMR1), a common non-integrin surface protein on many cell types.


CONCLUSIONS
At least 3 of the 4 dengue serotypes interact with the 37 kDa/67 kDa laminin receptor, LAMR1, which may be a common player in dengue virus-cell surface interaction.
Tio
Phaik Hooi
PH
Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia. phtio@yahoo.com
Jong
Wan Wui
WW
Cardosa
Mary Jane
MJ
D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000378:metabolism*; D005786:Gene Expression Regulation; D034921:Lamin Type B; D011485:Protein Binding; D011869:Radioligand Assay; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014764:Viral Proteins
D034921:Lamin Type B; D017474:Receptors, Laminin; D014764:Viral Proteins