[Genetic polymorphisms of fifteen short tandem repeat loci in Chengdu Han population].
OBJECTIVE
To acquire the population genetic data of fifteen short tandem repeat (STR) loci in Chengdu Han population.


METHODS
A total of 210 EDTA-blood specimens were collected from the unrelated individuals in Chengdu Han population. The DNA samples were extracted with Chelex method and amplified by multiplex PCR technique. The PCR products were analyzed by an automatic genetic analyzer; the relative fragment's lengths of PCR products were calculated by gene scan analysis software and afterward genotyped by genotype software.


RESULTS
Fifteen STR loci of the 210 samples showed a successful result of genotyping. The heterozygosities of the fifteen STR loci in Chengdu Han population were found to be 0.529-0.881; the combined exclusion probability and discrimination power for the fifteen STR loci in Chengdu Han population were determined to be 0.999998 and 7.3 x 10 (-17); respectively.


CONCLUSIONS
The distinct genotype of fifteen STR loci and the sex of sample could be unveiled just through PCR and electrophoresis once, and a higher measured value could be obtained for both the combined discrimination power and the exclusion probability; the fifteen STR loci can meet the needs of the parentage testing and personal identification in forensic medicine.
Li
Yingbi
Y
School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China. liyingbiscu@163.com
Wu
Jing
J
Hou
Yiping
Y
Zhang
Ji
J
Liao
Miao
M
Zhang
Lin
L
Chen
Guodi
G
D044466:Asian People / Q000235:genetics; D002681:China; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*