title: The reciprocal association of adipocytokines with insulin resistance and C-reactive protein in clinically healthy men.
abstract: In experimental models, adiponectin improves and tumor necrosis factor alpha (TNF- alpha ) impairs insulin action, and the expression of these adipocytokines seems to have a reciprocal regulation. The aim was to examine whether in a cross-sectional study, associations supporting this concept may be found in 58-year-old clinically healthy men, and also the relation to C-reactive protein (CRP). In 102 men, euglycemic hyperinsulinemic clamp was used to assess glucose infusion rate (GIR). Total body fat (dual-energy x-ray absorptiometry), plasma adiponectin (radioimmunoassay), TNF-alpha , and CRP (enzyme-linked immunosorbent assay) were measured. Adiponectin correlated positively to GIR (r=0.33, P<.001) and negatively to total fat mass (r=-0.29, P=.004), whereas TNF- alpha showed reverse associations (r=-0.31, P<.01, and r=0.31, P<.01). Adiponectin and TNF- alpha were negatively correlated (-0.28, P=.006). An interaction term (TNF- alpha /adiponectin ratio) and body fat together explained 31.3% (P<.001) in GIR variability. The odds ratio for having insulin resistance was 9.3 (95% CI, 2.2-38.9) in subjects with TNF-alpha values above and adiponectin levels below the median, as compared to subjects with TNF- alpha values below and adiponectin levels above the median. Total fat and TNF-alpha , but not adiponectin, were significantly associated with log CRP (R2=20%, P<.001). In conclusion, this study in man showed that plasma adiponectin and TNF-alpha were independently and reversely associated with insulin resistance. C-reactive protein levels were related to TNF-alpha and obesity.
authors.affiliation: Institute of Internal Medicine, Sahlgrenska University Hospital, Göteborg, Sweden. carljohan.behre@wlab.gu.se
authors.forename: Carl Johan
authors.initials: CJ
authors.lastname: Behre
authors.forename: Björn
authors.initials: B
authors.lastname: Fagerberg
authors.forename: Lillemor Mattsson
authors.initials: LM
authors.lastname: Hultén
authors.forename: Johannes
authors.initials: J
authors.lastname: Hulthe
mesh_terms: D015502:Absorptiometry, Photon; D052242:Adiponectin; D000273:Adipose Tissue; D001823:Body Composition; D002097:C-Reactive Protein / Q000032:analysis*; D003430:Cross-Sectional Studies; D015309:Glucose Clamp Technique; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood; D016017:Odds Ratio; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D052242:Adiponectin; D036341:Intercellular Signaling Peptides and Proteins; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein