title: Differential expression and cytoplasm/membrane distribution of endoglin (CD105) in human tumour cell lines: Implications in the modulation of cell proliferation.
abstract: Endoglin (CD105, an accessory component of the TGF-beta receptor complex) expression and distribution on different human tumour cells and its role in cellular proliferation were evaluated. We examined: 1) sixteen human carcinoma cell lines, 2) eight human sarcoma cell lines, 3) five miscellaneous tumour cell lines. HECV (endothelial cells) were employed as a positive control for endoglin expression. Normal Human Dermal Fibroblasts (NHDF) and 293 cells (epithelial kidney cells) were used as normal controls for connective and epithelial tissues, respectively. The results showed that CD105 was poorly expressed in the majority of human carcinoma cells (10/16), whereas it was highly expressed in most human sarcoma cells (7/8), and differently expressed by miscellaneous tumour cell lines. These data reflect endoglin expression by the normal counterparts of tumour cell lines, i.e. NHDF and 293 cells. However, CD105 levels in sarcoma cell lines, even though consistently lower than in NHDF, were significantly higher than those observed in carcinoma cells. Interestingly, CD105 presented a strong expression in the cytoplasm of MDA-MB-453 (breast carcinoma), NPA (papillary thyroid carcinoma), COLO-853 (melanoma) and SaOS-2 (osteosarcoma), but was weakly expressed on their cell membrane. This differential expression in the cytoplasm and on the membrane of some tumour cells, suggests a complex mechanism of translocation for this protein. The analysis of clonal growth in soft agar of some cell lines, characterized by high CD105 expression, showed an increased colony formation potential that was antagonized by the addition of anti-CD105 blocking mAb. The results indicated that endoglin is differentially expressed in human carcinoma and sarcoma cells and its overexpression modulates the proliferative rate of human solid tumour cells. Moreover, these data suggest that CD105 is involved in the regulation of TGF-beta effects in human solid malignancies, and therefore it could play an important role in tumour diagnosis and treatment.
authors.affiliation: Department of Molecular Biology and Cell Pathology, 'Federico II' University, I-80131 Naples, Italy. lorposti@unina.i
authors.forename: L
authors.initials: L
authors.lastname: Postiglione
authors.forename: G
authors.initials: G
authors.lastname: Di Domenico
authors.forename: M
authors.initials: M
authors.lastname: Caraglia
authors.forename: M
authors.initials: M
authors.lastname: Marra
authors.forename: G
authors.initials: G
authors.lastname: Giuberti
authors.forename: L
authors.initials: L
authors.lastname: Del Vecchio
authors.forename: S
authors.initials: S
authors.lastname: Montagnani
authors.forename: M
authors.initials: M
authors.lastname: Macri
authors.forename: E M
authors.initials: EM
authors.lastname: Bruno
authors.forename: A
authors.initials: A
authors.lastname: Abbruzzese
authors.forename: G
authors.initials: G
authors.lastname: Rossi
mesh_terms: D015703:Antigens, CD; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002462:Cell Membrane; D049109:Cell Proliferation; D003593:Cytoplasm; D000071063:Endoglin; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D011956:Receptors, Cell Surface; D012509:Sarcoma / Q000235:genetics* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D016212:Transforming Growth Factor beta; D019010:Vascular Cell Adhesion Molecule-1