[Purification of human serum apolipoprotein A I and A II by middle-pressure liquid chromatography].
OBJECTIVE
This study is made for the purification of human HDL apolipoproteins by middle-pressure liquid chromatography (MPLC).


METHODS
Human HDL was isolated by one step density ultracentrifugation. Delipided human HDL was separated by Sephacryl S-200 molecular sieve chromatography. Sephadex G-75 molecular sieve chromatography was used to separate apoA I and apoA II.


RESULTS
Purified apoA I and apoA II were obtained and SDS-PAGE and immunodiffusion test indicated that the proteins are the same as those theoretically predicted.


CONCLUSIONS
We have established a purification procedure for human HDL apolipoproteins with high efficiency and simplicity by MPLC. It could serve as a base for preclinical and clinical trials of HDL apolipoproteins.
Xin
Yan-fei
YF
Apolipoprotein Research Unit, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liu
Bing-wen
BW
Zhang
Zu-hui
ZH
D016632:Apolipoprotein A-I / Q000032:analysis / Q000302:isolation & purification*; D016633:Apolipoprotein A-II / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008075:Lipoproteins, HDL / Q000302:isolation & purification
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D008075:Lipoproteins, HDL