In vivo antitumor activity by 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone in a solid human carcinoma xenograft model.
Previously we have shown that 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), which is isolated from the buds of Cleistocalyx operculatus, significantly inhibits the growth of human liver cancer SMMC-7721 cells and is able to induce apoptosis of SMMC-7721 cells in vitro. Here we report the antitumor effects of DMC in vivo, using a solid human tumor xenograft mouse model using human liver cancer SMMC-7721 cells. The average tumor weights in the control group and in mice injected with 150 mg/kg DMC were 1.42+/-0.11 g and 0.59+/-0.12 g, respectively. Flow cytometric analysis of the tumor cell population demonstrated an aneuploid peak (representing 33.60+/-0.80% of the total in mice injected with 150 mg/kg DMC). To our knowledge, this is the first time that chalcone compounds have been applied to a human tumor xenograft model.
Ye
Chun-Lin
CL
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Liu
Jian-Wen
JW
Wei
Dong-Zhi
DZ
Lu
Yan-Hua
YH
Qian
Feng
F
D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D047188:Chalcones; D004195:Disease Models, Animal; D004354:Drug Screening Assays, Antitumor; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D014183:Transplantation, Heterologous
C501649:2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; D047188:Chalcones; D010936:Plant Extracts; D002599:Chalcone