title: Brain-derived neurotrophic factor promotes neurite growth and survival of antennal lobe neurons in brain from the silk moth, Bombyx mori in vitro.
abstract: This study was conducted to investigate effects of brain-derived neurotrophic factor on the neurite growth and the survival rate of antennal lobe neurons in vitro, and secretion of brain-derived neurotrophic factor-like neuropeptide from brain into hemolymph in the silk moth, Bombyx mori. In primary culture of antennal lobe neurons with brain-derived neurotrophic factor, it promoted both a neurite extension of putative antennal lobe projection neurons and an outgrowth of branches from principal neurites of putative antennal interneurons with significance (p<0.05). Brain-derived neurotrophic factor also increased significantly a survival rate of antennal lobe neurons (p<0.05). Results from immunolabeling of brain and retrocerebral complex, and ELISA assay of hemolymph showed that brain-derived neurotrophic factor-like neuropeptide was synthesized by both median and lateral neurosecretory cells of brain, then transported to corpora allata for storage, and finally secreted into hemolymph for action. These results will provide valuable information for differentiation of invertebrate brain neurons with brain-derived neurotrophic factor.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Dong Kyung
authors.initials: DK
authors.lastname: Sung
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Park
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Park
authors.forename: Cheolin
authors.initials: C
authors.lastname: Park
authors.forename: Soung-Hoo
authors.initials: SH
authors.lastname: Jeon
authors.forename: Pil Don
authors.initials: PD
authors.lastname: Kang
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006458:Hemolymph / Q000378:metabolism; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D007797:Laminin / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009475:Neurons, Afferent / Q000378:metabolism / Q000502:physiology*; D009479:Neuropeptides / Q000097:blood; D012701:Serotonin / Q000494:pharmacology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D007797:Laminin; D009479:Neuropeptides; D003208:Concanavalin A; D012701:Serotonin; D006735:Horseradish Peroxidase