[The effect of ultraviolet B on interleukin-8 secretion in human keratinocyte cell line].
OBJECTIVE
To investigate the effect of ultraviolet B (UVB) on Interleukin-8 secretion in human keratinocyte cell line.


METHODS
The concentration of IL-8 was detected by ELISA kit 24 h after human keratinocytes (HKC) were irradiated by different doses of UVB, and the level of IL-8 was also determined at different times after the same dose of UVB irradiation.


RESULTS
The secretion of IL-8 was increased after the HKC were irradiated by UVB, the effect was dose-dependent when UVB ranged from 10 to 40 mJ/cm2, and there was statistically significant difference between the IL-8 level of UVB groups (20-70 mJ/cm2) and the control (0 mJ/cm2) (P<0.01). The level of IL-8 was increased 1 h after the irradiation of 30 mJ/cm2 UVB, and it reached the peak at 12 h. There was statistically significant difference between the IL-8 levels detected at different times (3 h, 6 h, 12 h, 24 h after irradiation) and that at 0 h (P<0.01).


CONCLUSIONS
UVB increase the secretion of IL-8, and the effect is dose-dependent to some extent.
Jiang
Xian
X
Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang
Guo-xing
GX
Zhou
Min-yan
MY
Feng
Yun
Y
Wu
Qi
Q
Ran
Yu-ping
YP
D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism* / Q000528:radiation effects; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
D016209:Interleukin-8