[Effects of Radix Salviae Miltiorrhizae on Ca2+ in hepatic stellate cells].
OBJECTIVE
To investigate the effects of Radix Salviae Miltiorrhizae (RSM) on intracellular free calcium in hepatic stellate cells (HSCs).


METHODS
After the model of hepatic fibrosis was established in SD rats, RSM [20 ml/(kg x d)] was given via gastrogavage to the rats of treatment groups while the same volume of 0.9% NaCl was given to the rats of control groups twice a day for 6 consecutive days. Then the blood sample was drawn from the inferior vena cava, and the serum was extracted for pharmacological studies. After 24 h incubation with 10% drug serum, HSCs were loaded with Fluo-3/AM, a Ca2+ marker, and were observed with laser scanning confocal microscopy (LSCM).


RESULTS
By comparison with controls, both RSM pharmacological serums decreased [Ca2+]i in HSCs significantly in the condition of using Ang II or not (P<0.05).


CONCLUSIONS
RSM decreased [Ca2+]i in activated HSCs, which may be one of important ways to block liver fibrosis.
Wang
Jun-min
JM
Department of Gastroenterology, Hebei Medical University Affiliated Second Hospital, Shijiazhuang 050000, China.
Yao
Xi-xian
XX
Li
Xiao-tian
XT
Yang
Shu-liang
SL
Su
Zheng
Z
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D015398:Signal Transduction / Q000187:drug effects
D015220:Calcium Channels; D004365:Drugs, Chinese Herbal; D000804:Angiotensin II; D002118:Calcium