title: Identification of 40LoVe, a Xenopus hnRNP D family protein involved in localizing a TGF-beta-related mRNA during oogenesis.
abstract: Asymmetric distribution of cellular components underlies many biological processes, and the localization of mRNAs within domains of the cytoplasm is one important mechanism of establishing and maintaining cellular asymmetry. mRNA localization often involves assembly of large ribonucleoproteins (RNPs) in the cytoplasm. Using an RNA affinity chromatography approach, we investigated localization RNP formation on the vegetal localization element (VLE) of the mRNA encoding Vg1, a Xenopus TGF-beta family member. We identified 40LoVe, an hnRNP D family protein, as a specific VLE binding protein from Xenopus oocytes. Interaction of 40LoVe with the VLE strictly correlates with the ability of the RNA to localize, and antibodies against 40LoVe inhibit vegetal localization in vivo in oocytes. Our results associate an hnRNP D protein with mRNA localization and have implications for several functions mediated by this important protein family.
authors.affiliation: EMBL Gene Expression Programme, Meyerhofstrasse 1, Heidelberg 69117, Germany.
authors.forename: Kevin
authors.initials: K
authors.lastname: Czaplinski
authors.forename: Thomas
authors.initials: T
authors.lastname: Köcher
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Schelder
authors.forename: Alexandra
authors.initials: A
authors.lastname: Segref
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilm
authors.forename: Iain W
authors.initials: IW
authors.lastname: Mattaj
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D009866:Oogenesis / Q000502:physiology*; D010802:Phylogeny; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D016415:Sequence Alignment; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
chemical_list: C513103:GDF1 protein, Xenopus; D006023:Glycoproteins; C544068:HNRNPAB protein, Xenopus; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins