title: New antibacterial peptide derived from bovine hemoglobin.
abstract: Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis after separation by reversed-phase HPLC. From this fraction a pure peptide was isolated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). This peptide correspond to the 107-136 fragment of the alpha chain of bovine hemoglobin. The minimum inhibitory concentrations (MIC) towards the four strains and its hemolytic activity towards bovine erythrocytes were determined. A MIC of 38 microM was reported against L. innocua and 76 microM for other various bacterial species. This peptide had no hemolytic activity up to 380 microM concentration.
authors.affiliation: Laboratoire de Technologie des Substances Naturelles, IUT A Lille I, BP 179, 59653 Villeneuve d'Ascq Cedex, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Daoud
authors.forename: Veronique
authors.initials: V
authors.lastname: Dubois
authors.forename: Loredana
authors.initials: L
authors.lastname: Bors-Dodita
authors.forename: Naima
authors.initials: N
authors.lastname: Nedjar-Arroume
authors.forename: Francois
authors.initials: F
authors.lastname: Krier
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Chihib
authors.forename: Patrice
authors.initials: P
authors.lastname: Mary
authors.forename: Mostafa
authors.initials: M
authors.lastname: Kouach
authors.forename: Gilbert
authors.initials: G
authors.lastname: Briand
authors.forename: Didier
authors.initials: D
authors.lastname: Guillochon
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D004926:Escherichia coli / Q000187:drug effects; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006461:Hemolysis; D006868:Hydrolysis; D008087:Listeria / Q000187:drug effects; D016982:Micrococcus luteus / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012477:Salmonella enteritidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides; C503955:hemoglobin (107-136), bovine