First microsatellite loci of red mullet (Mullus barbatus) and their application to genetic structure analysis of Adriatic shared stock.
In order to study the genetic structure of the Adriatic shared stock of red mullet (Mullus barbatus), we developed a set of dinucleotide microsatellite markers. A dinucleotide-enriched genomic library was obtained, and 6 polymorphic dinucleotide loci were successfully optimized. The markers showed high expected heterozygosity (from 0.68 to 0.92) and allele number (from 12 to 33); thus they appear to be suitable for detecting genetic differences in the population of red mullet. Four Adriatic samples were subsequently analyzed for microsatellite variation, and the results showed subtle but statistically significant genetic differentiation, indicating that the Adriatic red mullet may group into local, genetically isolated populations. No correlation between geographic distance and genetic differentiation was observed. In addition, the evidence of recent bottlenecks in the Adriatic samples indicates that the observed population subdivision might reflect random local allelic variations, generated by reproductive success, survival rates, or fishing pressure.
Garoia
Flavio
F
Molecular Genetics for Environmental and Fishery Resources Laboratory, Interdept. Center for Research in Environmental Sciences, University of Bologna, Ravenna, Italy.
Guarniero
Ilaria
I
Piccinetti
Corrado
C
Tinti
Fausto
F
D000818:Animals; D017931:DNA Primers; D018900:Dinucleotide Repeats / Q000235:genetics*; D005787:Gene Frequency; D015723:Gene Library; D014644:Genetic Variation; D005828:Genetics, Population; D008522:Mediterranean Sea; D008957:Models, Genetic; D010473:Perciformes / Q000235:genetics*; D011157:Population Dynamics; D013045:Species Specificity
D017931:DNA Primers