title: Nitric oxide induces apoptosis in mouse C2C12 myoblast cells.
abstract: To investigate whether nitric oxide (NO) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (SNP), NO donor, on the apoptosis of mouse C2C12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme assay. Mouse C2C12 myoblast cells treated with SNP exhibited several apoptotic features. SNP increased p53 expression and bax expression. SNP also enhanced caspase-3 enzyme activity. The data show that NO may induce apoptotic cell death in myoblast cells through the activation of p53-, bax-, and caspase-dependent intracellular death-related pathways.
authors.affiliation: Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Myoung-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Sang-Wan
authors.initials: SW
authors.lastname: Han
authors.forename: Seong-Yeon
authors.initials: SY
authors.lastname: Cho
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D020287:In Situ Nick-End Labeling; D007211:Indoles; D051379:Mice; D008856:Microscopy, Fluorescence; D032446:Myoblasts / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors; D009599:Nitroprusside / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein
chemical_list: C494827:Bax protein, mouse; D004792:Enzyme Precursors; D005456:Fluorescent Dyes; D007211:Indoles; D020030:Nitric Oxide Donors; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D009599:Nitroprusside; D009569:Nitric Oxide; C007293:DAPI; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases