title: [The influence of dermal template on the expressions of signal transduction protein Smad 3 and transforming growth factor beta1 and its receptor during wound healing process in patients with deep burns].
abstract: OBJECTIVE
  To investigate the influence of dermal template on the expressions of signal transduction protein Smad 3 and transforming growth factor beta1 and its receptor during wound healing process in patients with deep burns.
  
  
  METHODS
  Twenty burn patients with excision of full thickness burn in the extremities were enrolled in the study and divided into two groups, i.e. template interfering group (E, n = 20, grafting of dermal template [allogeneic acellular dermal matrix] with razor thin autoskin) and control group (C, n = 20, grafting of razor thin autoskin only). The contralateral side served as the self-control. Tissue samples from the burn wounds were harvested at 1, 2, 3 and 4 post-operative weeks (POW) for immunohistochemistry staining. The positive expression rates of TGF-beta1, TbetaRI, TbetaRII and Smad3 proteins were determined by image analysis system.
  
  
  RESULTS
  The positive expressions of TGFbeta1, TbetaRI, TbetaRII and signal transduction protein Smad 3 in the tissue samples in both groups could be identified during 1 approximately 4 POW, and they diminished thereafter with the process of wound healing. The expression rate of TGF-beta1 in E group was (13.08 +/- 4.65)% at 1 POW and (9.03 +/- 1.89)% at 4 POW. The positive expression rate of above indices in E group was obviously lower than that in C group in corresponding time points (P < 0.05).
  
  
  CONCLUSIONS
  The expression levels of TGFbeta1, TbetaRI, TbetaRII and Smad 3 protein in deep burn wounds could be lowered by mixed grafting of dermal template with razor thin autoskin, which might be beneficial in ameliorating of scar hyperplasia in the burn wound.
authors.affiliation: Department of Burns, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.forename: Xi-Qiao
authors.initials: XQ
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Qing
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Liao
authors.forename: Shu-Liang
authors.initials: SL
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000378:metabolism* / Q000601:surgery; D020405:Dermis / Q000637:transplantation*; D006801:Humans; D008875:Middle Aged; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis*; D015398:Signal Transduction; D016038:Skin Transplantation; D051900:Smad3 Protein / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D014183:Transplantation, Heterologous; D014945:Wound Healing
chemical_list: D018125:Receptors, Transforming Growth Factor beta; C497790:SMAD3 protein, human; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1