title: Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells.
abstract: Cytokine-induced differentiation of myeloid leukemia cells has important therapeutic implications, but the mechanism remains to be clarified. M1 cell, a mouse acute myeloid leukemia cell line, which underwent growth inhibition, terminal differentiation and apoptosis in response to IL-6, was selected as an experimental model to study on the molecular mechanisms of myeloid cell differentiation on a proteome-wide scale. Cell differentiation was evaluated by cell morphology and CD11b expression. With two-dimensional (2D) gel analyses, 17 protein spots showed obvious changes in quantity during the process of differentiation were found. With matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) or/and nano-electrospray ionization MS/MS (ESI-MS/MS) analysis, 15 protein spots were identified. The mRNA levels of these 15 proteins during differentiation were also examined using a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Except two proteins, the mRNA levels demonstrated similar expression patterns to what the proteomic analysis revealed. The identified proteins were known to be involved in different cellular functions, including protein synthesis, transcription, signal transduction, cell cycle control, cell rescue and defense, cellular organization, and metabolism. Notably, seven proteins were not described before to be involved in differentiation. Our data provide novel information for a better understanding of the mechanisms by which terminal differentiation of acute myeloid leukemia cells induced by IL-6.
authors.affiliation: Department of Molecular Immunology, Beijing Institute of Basic Medical Science, TaiPing Road 27, Beijing 100850, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Zhang
authors.forename: Bing-Ying
authors.initials: BY
authors.lastname: Liu
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Lv
authors.forename: Mei-Ru
authors.initials: MR
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Feng
authors.forename: Song-Cheng
authors.initials: SC
authors.lastname: Yang
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
authors.forename: Bei-Fen
authors.initials: BF
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D039481:CD11b Antigen / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D055313:Cystatin B; D015891:Cystatins / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis; D015850:Interleukin-6 / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology*; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D010544:Peroxidases / Q000096:biosynthesis; D054464:Peroxiredoxins; D040901:Proteomics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D039481:CD11b Antigen; C524992:Cstb protein, mouse; D015891:Cystatins; D015850:Interleukin-6; D008565:Membrane Proteins; D011994:Recombinant Proteins; D055313:Cystatin B; D010544:Peroxidases; D054464:Peroxiredoxins; D005634:Fructose-Bisphosphate Aldolase