title: Expression of Duffy antigen receptor for chemokines during reticulocyte maturation: using a CD71 flow cytometric technique to identify reticulocytes.
abstract: Flow cytometric methods commonly used to identify reticulocytes are of limited usefulness in malarious areas, since RNA staining also detects plasmodia. An important antigen expressed on reticulocytes is Duffy antigen receptor for chemokines (DARC, also known as Fy), the receptor for Plasmodium vivax. An early marker for reticulocytes is CD71 (transferrin receptor). We have been interested in CD71 as an alternative marker for reticulocytes in the context of Fy expression. Flow cytometry was used to determine the expression of Fy on CD71-positive and -negative reticulocytes and to correlate serology and genotype. A reduction of 13 percent was seen in Fy6 expression between CD71-positive reticulocytes and RNA-positive reticulocytes. CD71 disappears early during reticulocyte maturation, while Fy6 expression is relatively preserved. CD71 is an alternative to staining for RNA for reticulocyte assays relating to Fy6 expression.
authors.affiliation: Department of Infectious Diseases & Clinical Epidemiology, Monash Medical Centre, Clayton, Victoria 3168, Australia.
authors.forename: I J
authors.initials: IJ
authors.lastname: Woolley
authors.forename: E M
authors.initials: EM
authors.lastname: Wood
authors.forename: R M
authors.initials: RM
authors.lastname: Sramkoski
authors.forename: P A
authors.initials: PA
authors.lastname: Zimmerman
authors.forename: J P
authors.initials: JP
authors.lastname: Miller
authors.forename: J W
authors.initials: JW
authors.lastname: Kazura
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D016922:Cellular Senescence / Q000276:immunology*; D004375:Duffy Blood-Group System / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D016130:Immunophenotyping; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics; D011990:Receptors, Transferrin; D012156:Reticulocytes / Q000166:cytology* / Q000276:immunology
chemical_list: C473745:ACKR1 protein, human; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015415:Biomarkers; C068126:CD71 antigen; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin