Application of bubble separation for quantitative analysis of choline in Dioscorea (yam) tubers.
A modified assay based on the AACC official method 86-45 (AACC, 2000) for the determination of choline in three cereals and three varieties of Dioscorea (yam) tubers was developed. When tested in wheat, rice, and oat flour, choline estimated by the modified method was 34.0-45.3% higher than that of the original AACC method. In a system with higher contents of starch and mucilage, such as Dioscorea (yam) tubers, extra procedures in sample preparation needed to be carried out to separate starch and mucilage. The choline contents of the following Dioscorea (yam) tubers using the original AACC method and the present modified AACC method through coupling an additional bubble separation procedure, respectively, were (mean +/- SD, mg/g solid) Keelung yam (D. pseudojaponicaY.) 0.92 +/- 0.09 and 2.21 +/- 0.12, Yangmingshan yam (D. alata L.) 0.77 +/- 0.09 and 1.78 +/- 0.28, and Ming-Chien yam (D. purpurea) 0.44 +/- 0.09 and 1.35 +/- 0.19. Choline was 231-306% higher than when the original AACC method was used. Dioscorea (yam) tubers were much higher in choline content than they were in cereals. Bubble separation is an appropriate procedure in the practice for the maximum assay of choline in yams. It is accurate, rapid, easy to handle, and especially good for recovering choline from a starch and polysaccharide-protein-containing system.
Fu
Yi-Chung
YC
Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung City, 40227, R.O.C., Taiwan.
Chen
Shiau-Huei
SH
Huang
Pau-Yau
PY
Li
Yun-Ju
YJ
D000269:Adhesives / Q000302:isolation & purification; D018554:Avena / Q000737:chemistry; D002794:Choline / Q000032:analysis*; D027742:Dioscorea / Q000737:chemistry*; D005433:Flour / Q000032:analysis; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D012275:Oryza / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013213:Starch / Q000302:isolation & purification; D014908:Triticum / Q000737:chemistry
D000269:Adhesives; D013213:Starch; D002794:Choline