Antioxidant defence during cardiopulmonary bypass surgery.
OBJECTIVE
Cardiac surgery may lead to severe oxidative stress due to formation of oxidation products generated during ischemia and reperfusion. We investigated to which extent oxidative stress influences a number of endogenous antioxidants and markers of cellular activation.


METHODS
At six time points blood was withdrawn from patients undergoing coronary artery bypass grafting, using the on-pump procedure.


RESULTS
Both glutathione peroxidase and superoxide dismutase show a gradual and strong increase in activity during surgery (40 and 30%, respectively), returning to baseline values 24 h after surgery. The total antioxidant capacity has a maximum increase of 60%. Markers of cellular activation, such as eosinophil cationic protein and tryptase also increase during the procedure.


CONCLUSIONS
Cardiac surgery results in systemic inflammation accompanied or caused by severe oxidative stress. The human body has a strong innate oxidative defence screen, which is probably not sufficient to fully compensate for the total amount of oxidative damage.
Luyten
Chris R
CR
Department of Respiratory Medicine, University of Antwerp, Universiteitsplein 1, B-2610 Antwerpen-Wilrijk, Belgium.
van Overveld
Frans J
FJ
De Backer
Lieve A
LA
Sadowska
Anna M
AM
Rodrigus
Inez E
IE
De Hert
Stefan G
SG
De Backer
Wilfried A
WA
D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D047150:Eosinophil Cationic Protein / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011184:Postoperative Period; D012697:Serine Endopeptidases / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D053802:Tryptases
D000975:Antioxidants; D015415:Biomarkers; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D047150:Eosinophil Cationic Protein; D012697:Serine Endopeptidases; D053802:Tryptases