title: Oxygenation alters ganglioside expression in rat liver following partial hepatectomy.
abstract: Gangliosides from livers of weanling rats were analyzed after 15% partial hepatectomy (PH) and different pre- and post-operative hyberbaric oxygenation (pre- and postHBO). Neu5Ac was the predominant ganglioside-derived sialic acid (>85%) compared to Neu5Gc. Almost identical low total sialic acid content (Neu5Ac+Neu5Gc) of the control and operated nonHBO animals opposed a 6.4- to 7.6-fold increase in pre- and postHBO animals (69.26 and 81.64pmol/mg wet weight, respectively). NanoESI-QTOF mass spectrometry combined with HPTLC immunostaining revealed GM3(Neu5Ac) and GM3(Neu5Gc) as major gangliosides, correlating with the respective sialic acid concentrations. Minor neolacto-series gangliosides were enhanced in preHBO and postHBO, but GM1-core gangliosides only in preHBO rats. GM2 and GalNAc-GM1b were clearly detectable in oxygenated rats compared to traces in the control and nonHBO animals. These results point at a functional role of gangliosides in liver growth regulation and reconstitution after PH combined with pre- and post-operative HBO treatment.
authors.affiliation: Department of Biochemistry, Split University Medical School, Soltanska 2, 21000 Split, Croatia. markotic@bsb.mefst.hr <markotic@bsb.mefst.hr>
authors.forename: Anita
authors.initials: A
authors.lastname: Markotić
authors.forename: Vedrana Cikes
authors.initials: VC
authors.lastname: Culić
authors.forename: Tina Ticinović
authors.initials: TT
authors.lastname: Kurir
authors.forename: Iris
authors.initials: I
authors.lastname: Meisen
authors.forename: Heino
authors.initials: H
authors.lastname: Büntemeyer
authors.forename: Vesna
authors.initials: V
authors.lastname: Boraska
authors.forename: Tatijana
authors.initials: T
authors.lastname: Zemunik
authors.forename: Nadan
authors.initials: N
authors.lastname: Petri
authors.forename: Marko
authors.initials: M
authors.lastname: Mesarić
authors.forename: Jasna
authors.initials: J
authors.lastname: Peter-Katalinić
authors.forename: Johannes
authors.initials: J
authors.lastname: Müthing
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005732:Gangliosides; D010100:Oxygen