Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress.
DNA microarray was used as a tool to investigate genome-wide transcriptional responses of Yersinia pestis to hyperosmotic and high-salinity stress. Hyperosmotic stress specifically upregulated genes responsible for ABC-type transport and the cytoplasmic accumulation of certain polysaccharides, while high-salinity stress induced the transcription of genes encoding partition proteins and several global transcriptional regulators. Genes whose transcription was enhanced by both kinds of stress comprised those encoding osmoprotectant transport systems and a set of virulence determinants. The number of genes downregulated by the two kinds of stress was much lower than that of upregulated genes, suggesting that neither kind of stress severely depresses cellular processes in general. Many differentially regulated genes still exist whose functions remain unknown. Y. pestis recognized high-salinity and hyperosmotic stress as different kinds of environmental stimuli, and different mechanisms enabled acclimation to these two kinds of stress, although Y. pestis still executed common mechanisms to accommodate both types of stress.
Han
Yanping
Y
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
Zhou
Dongsheng
D
Pang
Xin
X
Zhang
Ling
L
Song
Yajun
Y
Tong
Zongzhong
Z
Bao
Jingyue
J
Dai
Erhei
E
Wang
Jin
J
Guo
Zhaobiao
Z
Zhai
Junhui
J
Du
Zongmin
Z
Wang
Xiaoyi
X
Wang
Jian
J
Huang
Peitang
P
Yang
Ruifu
R
D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic; D014158:Transcription, Genetic / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000502:physiology
D012329:RNA, Bacterial; D012462:Saline Solution, Hypertonic