title: Surface-induced unfolding of human lactoferrin.
abstract: We have determined the structural conformations of human lactoferrin adsorbed at the air/water interface by neutron reflectivity (NR) and its solution structure by small angle neutron scattering (SANS). The neutron reflectivity measurements revealed a strong structural unfolding of the molecule when adsorbed at the interface from a pH 7 phosphate buffer solution (PBS with a total ionic strength at 4.5 mM) over a wide concentration range. Two distinct regions, a top dense layer of 15-20 angstroms on the air side and a bottom diffuse layer of some 50 angstroms into the aqueous subphase, characterized the unfolded interfacial layer. At a concentration around 1 g dm(-3), close to the physiological concentration of lactoferrin in biological fluids, the adsorbed amount was 5.5 x 10(-8) mol m(-2) in the absence of NaCl, but the addition of 0.3 M NaCl reduced protein adsorption to 3.5 x 10(-8) mol m(-2). Although the polypeptide distributions at the interface remained similar, quantitative analysis showed that the addition of NaCl reduced the layer thickness. Parallel measurements of lactoferrin adsorption in D2O instead of null reflecting water confirmed the unfolded structure at the interface. Furthermore, the D2O data indicated that the polypeptide in the top layer was predominantly protruded out of water, consistent with it being hydrophobic. In contrast, the scattering intensity profiles from SANS were well described by a cylindrical model with a diameter of 47 angstroms and a length of 105 angstroms in the presence of 0.3 M NaCl, indicating a retention of the globular framework in the bulk solution. In the absence of NaCl but with the same amount of phosphate buffer, the length of the cylinder increased to some 190 angstroms and the diameter remained constant. The length increase is indicative of changes in distance and orientation between the bilobal monomers due to the change in charge interactions. The results thus demonstrate that the surface structural unfolding was caused by the exposure of the protein molecule to the unsymmetrical energetic balance following surface adsorption.
authors.affiliation: Biological Physics Group, School of Physics and Astronomy, the University of Manchester, Sackville Street Building, Sackville Street, Manchester M60 1QD, UK. j.lu@manchester.ac.uk
authors.forename: Jian R
authors.initials: JR
authors.lastname: Lu
authors.forename: Shiamalee
authors.initials: S
authors.lastname: Perumal
authors.forename: Xiubo
authors.initials: X
authors.lastname: Zhao
authors.forename: Fausto
authors.initials: F
authors.lastname: Miano
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Enea
authors.forename: Richard R
authors.initials: RR
authors.lastname: Heenan
authors.forename: Jeff
authors.initials: J
authors.lastname: Penfold
mesh_terms: D000327:Adsorption; D000388:Air; D002021:Buffers; D003903:Deuterium / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007781:Lactoferrin / Q000737:chemistry* / Q000502:physiology; D009502:Neutrons; D011485:Protein Binding; D017510:Protein Folding; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000494:pharmacology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D002021:Buffers; D013501:Surface-Active Agents; D014867:Water; D012965:Sodium Chloride; D003903:Deuterium; D007781:Lactoferrin