Use of housekeeping gene sequencing for species identification of viridans streptococci.
Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis-sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.
Kiratisin
Pattarachai
P
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Li
Li
L
Murray
Patrick R
PR
Fischer
Steven H
SH
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005941:Glucokinase / Q000235:genetics*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D034363:Viridans Streptococci / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; D005954:Glucosephosphate Dehydrogenase; D005941:Glucokinase