End binding protein-1 (EB1) complements microtubule-associated protein-1B during axonogenesis.
The different strains of microtubule-associated protein (MAP)-1B-deficient mice that have been generated appear to express different phenotypes. This variability could be the consequence of the distinct genetic backgrounds of the animals used to generate these lines. Certain proteins might be able to complement the deficit of MAP1B function in these mice. Therefore, we examined whether the concentrations of potential compensatory proteins varied among these mutant strains. In this way, we identified significant differences in the amounts of the microtubule-associated EB1 protein between two of these strains. Furthermore, in transfection studies, we demonstrated that the overexpression of end binding protein-1 (EB1) could facilitate axonogenesis in MAP1B-/- cells in which EB1 is normally weakly expressed. Thus, we suggest that EB1 could complement MAP1B function during neural development.
Jiménez-Mateos
Eva M
EM
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Paglini
Gabriela
G
González-Billault
Christian
C
Cáceres
Alfredo
A
Avila
Jesús
J
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D019556:COS Cells; D002454:Cell Differentiation / Q000502:physiology*; D002522:Chlorocebus aethiops; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D014644:Genetic Variation / Q000502:physiology; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D010641:Phenotype; D013045:Species Specificity; D014404:Tubulin / Q000378:metabolism
C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin; C085284:microtubule-associated protein 1B