title: Translocation of Bax in rat hepatocytes cultured with ferric nitrilotriacetate.
abstract: Hepatic fibrosis occurs after many years of iron overload in liver. An effective iron deposition model induced by ferric nitrilotriacetate (FeNTA) in cultured rat hepatocytes was assumed. It has been shown that treatment of rat hepatocytes with FeNTA lead to oxidative stress and hepatocyte apoptosis. Hepatocyte apoptosis can promote liver fibrosis. The mechanisms of hepatocyte apoptosis induced by FeNTA have not yet been fully elucidated. The present study demonstrated that FeNTA-induced hepatocyte apoptosis was related to Bax translocation, cytochrome c release, and caspase-3 activation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Nan Tong University 226001, Jiangsu Province, PR China. zhouyajun64@hotmail.com
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yajun
authors.forename: Chen
authors.initials: C
authors.lastname: Hongshan
authors.forename: Sha
authors.initials: S
authors.lastname: Baoxi
authors.forename: Yao
authors.initials: Y
authors.lastname: Dengbing
authors.forename: Shi
authors.initials: S
authors.lastname: Jianhua
authors.forename: Ge
authors.initials: G
authors.lastname: Xinshun
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Cao
authors.initials: C
authors.lastname: Yi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003124:Colorimetry; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005290:Ferric Compounds / Q000633:toxicity*; D005452:Fluoresceins; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007211:Indoles; D008297:Male; D008954:Models, Biological; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity*; D021381:Protein Transport / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein
chemical_list: C494828:Bax protein, rat; D005290:Ferric Compounds; D005452:Fluoresceins; D007211:Indoles; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C029569:diacetyldichlorofluorescein; C007293:DAPI; D045304:Cytochromes c; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate