[The expression of Fas after fluid percussion brain injury in rats].
OBJECTIVE
To inquire into the pathologic diagnosis and the dating of injuries of light closed encephalon injury.


METHODS
Wistar rats were hurt by fluid percussion, and were killed respectively at 15 min, 30 min, 1 h, 3 h, 6 h, 12 h, 1 d, 4 d, 7 d, and 14 d after injury. The expression of Fas in the cerebral cortex, thalamus, and hippocampi was detected by immunohistochemistry and the results were assessed by image analysis system.


RESULTS
The results showed that the expression of Fas could be detected in thirty minutes after injury, and then it increased significantly in three hours, reached apex in twelve hours after injury, decreased gradually in four days after injury, and returned to normal in 14 days after injury.


CONCLUSIONS
This research demonstrated that Fas-mediated apoptosis appeared not only around brain trauma but also in the brain tissue far away from the traumatic area. It indicted that Fas expression is a useful target for diagnosis of early brain injury and the regularity of Fas expression could be used as one of indications in deducing the time of brain injury.
Pan
Hong-fu
HF
Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wang
Ye
Y
Chen
Xiao-gang
XG
Yang
Jing
J
Li
Lei-bo
LB
Peng
Qi-yi
QY
Wang
Wen-dong
WD
Liu
Min
M
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D015415:Biomarkers; D019014:fas Receptor