title: [Purification and identification of recombinant varicella-zoster virus glycoprotein E].
abstract: OBJECTIVE
  To purify and identify recombinant Varicella-Zoster Virus Glycoprotein E.
  
  
  METHODS
  The recombinant plasmid pGEX-VZVgE was induced by isopropyl-beta-D-thiogalactoside (IPTG), the fusion protein was purified with affinity chromatography column; then the purified fusion protein was cleaved by thrombin, and the product's antigenicity was examined by Western blot.
  
  
  RESULTS
  The product of pGEX-VZVgE induced by IPTG was separated from the mixture proteins by the affinity chromatography column, the expressed fusion protein's relative molecular mass was about 98 x 10(3). After cleavage, the obtained VZV Glycoprotein E's relative molecular mass was about 72 x 10(3); the purified fusion protein and VZV Glycoprotein E were single band by SDS-PAGE. The available antigenicity of Glycoprotein E was confirmed by Western blot.
  
  
  CONCLUSIONS
  Purification of VZV Glycoprotein E with affinity chromatography is an effective method. It provides a foreground for studies on the application of VZV gE.
authors.affiliation: Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Fu-min
authors.initials: FM
authors.lastname: Li
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Ran
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: De-jian
authors.initials: DJ
authors.lastname: Duan
authors.forename: Xiu-hong
authors.initials: XH
authors.lastname: Zhao
authors.forename: Ju-ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Zhou
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity; D014645:Herpesvirus 3, Human; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C056913:glycoprotein E, varicella-zoster virus