Data analysis for a dual-channel virus counter.
A simple algorithm is presented for quantitative analysis of simultaneous events on a dual-channel flow cytometer designed specifically for virus counting. The algorithm, which is based on matrix analysis of burst lag times, was evaluated using baculovirus samples that had previously been quantified by the plaque titer method. The results indicated statistical reliability for the algorithm, with three of six samples yielding the same value, within error, for viruses per unit volume as the plaque titer. The other three samples yielded values within a factor approximately 2, which was deemed acceptable given the limitations of the plaque titer method.
Stoffel
Carrie L
CL
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA.
Rowlen
Kathy L
KL
D000465:Algorithms; D016367:Baculoviridae; D005453:Fluorescence; D005456:Fluorescent Dyes; D014773:Virology / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes