title: Dengue virus infections: comparison of methods for diagnosing the acute disease.
abstract: BACKGROUND
  The control of dengue depends solely on the control of the insect vector and efficient diagnosis of human cases as no vaccines or specific treatments are currently available. Existing diagnostic methods for suspected clinical cases are complicated by the short duration of viraemia and by serological cross-reactivity with epitopes from other flaviviruses.
  
  
  OBJECTIVE
  To evaluate PCR-based tests (nested reverse transcription (RT)-PCR and real-time RT-PCR) for the detection and serotyping of dengue virus and compare the results with those obtained with a widely used immunological test (IgM antibody capture ELISA-MAC-ELISA).
  
  
  CONCLUSIONS
  The PCR-based methods were more effective in the first few days of infection, whereas the MAC-ELISA became more sensitive 5 or 6 days after disease onset. These results suggest that the best method for dengue diagnosis is a combination of PCR-based and immunological tests. Real-time RT-PCR was more sensitive than the nested RT-PCR approach. Furthermore, it was rapid, reproducible and highly specific, making it a potential method for the diagnosis of dengue fever.
authors.affiliation: Instituto de Biologia Molecular do Paraná, IBMP, Rua Prof. Algacyr Munhoz Mader, 3775, 81350-010 Curitiba, Paraná, Brazil.
authors.forename: Celina
authors.initials: C
authors.lastname: de Oliveira Poersch
authors.forename: Daniela Parada
authors.initials: DP
authors.lastname: Pavoni
authors.forename: Mario H
authors.initials: MH
authors.lastname: Queiroz
authors.forename: Luana
authors.initials: L
authors.lastname: de Borba
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.forename: Claudia Nunes Duarte
authors.initials: CN
authors.lastname: dos Santos
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Krieger
mesh_terms: D000208:Acute Disease; D000914:Antibodies, Viral / Q000097:blood; D003715:Dengue / Q000097:blood / Q000175:diagnosis*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral