The invertor knock-in conditional chromosomal translocation mimic.
Knock-in models of tumor-specific chromosomal translocations can generate lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers.
Forster
Alan
A
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Pannell
Richard
R
Drynan
Lesley F
LF
Codrington
Rosalind
R
Daser
Angelika
A
Metzler
Markus
M
Lobato
M Natividad
MN
Rabbitts
Terence H
TH
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002469:Cell Separation; D002875:Chromosomes / Q000648:ultrastructure*; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D005821:Genetic Techniques; D006801:Humans; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000378:metabolism; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D018076:DNA, Complementary; D012313:RNA