The detection of oxycodone in meconium specimens.
A procedure for the determination of oxycodone in meconium using direct ELISA microplate technology followed by electron impact gas chromatography-mass spectrometry (GC-MS) is described for the first time. The abuse of oxycodone (OxyContin) has been widely discussed in mainstream media, and it has been described as a cheap form of heroin. Oxycodone has been reported as having a high degree of abuse and potential complications in neonates from maternal drug use. Using a standard enzyme multiplied immunoassay screening technology, the cross-reactivity of oxycodone to the morphine antibody is only 5-6%. A positive screening value would require a high concentration of drug to be present, so a protocol for the detection of oxycodone in meconium using a direct ELISA microplate immunoassay followed by GC-MS was developed. The assay is now routinely used in our laboratory.
Le
Ngoc L
NL
U.S. Drug Testing Laboratories, 1700 S. Mount Prospect Road, Des Plaines, Illinois 60018, USA.
Reiter
Andrea
A
Tomlinson
Kimberly
K
Jones
Joseph
J
Moore
Christine
C
D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D009020:Morphine / Q000276:immunology; D009294:Narcotics / Q000032:analysis* / Q000276:immunology; D010098:Oxycodone / Q000032:analysis* / Q000276:immunology
D009294:Narcotics; D009020:Morphine; D010098:Oxycodone