title: Granulocyte macrophage-colony stimulating factor improves impaired anastomotic wound healing in rats treated with intraperitoneal mitomycin-C.
abstract: OBJECTIVE
  Intraperitoneal chemotherapy (IPCT) delivers higher local concentrations of cytotoxic drugs than intravenous (i.v.) chemotherapy, but it can adversely affect the healing of intestinal anastomoses if given in the early postoperative period. Intestinal anastomotic leakage is a serious surgical complication. Experimental and clinical studies have shown that the local administration of granulocyte macrophage-colony stimulating factor (GM-CSF) improves would healing. Therefore, we evaluated the effects of locally applied GM-CSF on anastomotic wound healing in rats treated with intraperitoneal mitomycin-C immediately after surgery.
  
  
  METHODS
  We performed colon anastomoses in albino rats, which were then divided into three treatment groups. Group A was a control group that received no treatment, Group B was given intraperitoneal mitomycin-C postoperatively, and Group C was given intraperitoneal mitomycin-C with a local injection of GM-CSF postoperatively. We measured bursting pressures and hydroxyproline content, and histologically examined the resected anastomoses on postoperative day (POD) 3.
  
  
  RESULTS
  Anastomotic healing was impaired after intraperitoneal mitomycin-C, but this was overcome by the injection of GM-CSF into the perianastomotic area.
  
  
  CONCLUSIONS
  Local GM-CSF administration counteracts the detrimental effects of intraperitoneal mitomycin-C treatment on intestinal anastomoses in rats.
authors.affiliation: Department of General Surgery, Ankara Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Cetinkaya
authors.forename: Soykan
authors.initials: S
authors.lastname: Dinc
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Gulcelik
authors.forename: Nurten
authors.initials: N
authors.lastname: Renda
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ustun
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Caydere
authors.forename: Haluk
authors.initials: H
authors.lastname: Alagol
mesh_terms: D000704:Analysis of Variance; D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000503:physiopathology / Q000601:surgery*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D016685:Mitomycin / Q000633:toxicity*; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016685:Mitomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor