title: Enteral donor pre-treatment with ursodeoxycholic acid protects the liver against ischaemia-reperfusion injury in rats.
abstract: Liver donor pre-treatment with ursodeoxycholic acid (UDCA) may protect against injury during transplantation. In the present study we evaluated whether enteral administration of UDCA has an effect on bile flow and protects the liver from injury related to transplantation. Wistar rats were used in liver perfusion (LP) and transplantation (LTx) models. Rats were enterally administered UDCA (800 mg/kg) 3 h before cold perfusion. In LP, bile flow and bile acid composition were analysed. In LTx, serum ALT and liver histology were analysed. LP showed biliary UDCA enrichment up to 36+/-13% in pre-treated rats, causing higher bile flow (P = 0.026) compared with control rats. LTx showed lower ALT and TUNEL positive hepatocytes in the UDCA group (P < 0.02 and P < 0.05). In conclusion, augmented bile salt-dependent bile flow is preserved in the liver after cold storage. Enteral donor pre-treatment with UDCA protects the liver against ischaemia-reperfusion injury.
authors.affiliation: Department of Transplantation Surgery, Karolinska Institute, Huddinge University Hospital B56, 141 86 Stockholm, Sweden. grzegorz.nowak@cfss.ki.se
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Nowak
authors.forename: Ulf G
authors.initials: UG
authors.lastname: Norén
authors.forename: Annika
authors.initials: A
authors.lastname: Wernerson
authors.forename: Hanns-Ulrich
authors.initials: HU
authors.lastname: Marschall
authors.forename: Lennart
authors.initials: L
authors.lastname: Möller
authors.forename: Bo-Göran
authors.initials: BG
authors.lastname: Ericzon
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D002756:Cholagogues and Choleretics / Q000494:pharmacology*; D003080:Cold Temperature; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D019172:Transplantation Conditioning / Q000379:methods*; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
chemical_list: D002756:Cholagogues and Choleretics; D014580:Ursodeoxycholic Acid