Cytochrome P4501A induction in brown trout exposed to small streams of an urbanised area: results of a five-year-study.
This case study examines the ability of the cytochrome P4501A (CYP1A) biomarker to distinguish the pollution status of two small streams, Krähenbach and Körsch, receiving different levels of urban and agricultural impact, with low to moderate contamination by arylhydrocarbon receptor (AhR)-binding PAHs and PCBs. Brown trout, Salmo trutta, exposed in enclosure restrictions, showed significant between-stream differences of hepatic CYP1A levels. EROD activities were the better discriminator than CYP1A protein levels. The CYP1A response was consistent and repeatable over the 5-year observation period from 1995 to 1999. In contrast to brown trout, hepatic CYP1A of stone loach, Barbatula barbatula, did not clearly distinguish the streams. The findings of this long-term study lend support to the use of CYP1A as a biomarker of degraded environmental conditions, provided that sufficiently long observation periods are used to average out confounding factors, that sufficiently sensitive detection methods are used, and that a responsive monitoring species is chosen.
Behrens
Anja
A
Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research, Permoserstr. 15, D-04318 Leipzig, Germany.
Segner
Helmut
H
D000383:Agriculture; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002947:Cities; D003531:Cypriniformes / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D004789:Enzyme Activation; D004790:Enzyme Induction; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D045483:Rivers; D012680:Sensitivity and Specificity; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1