Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes.
Hydrophobin cDNA (fv-hyd1), which is specifically expressed during fruiting body development, was isolated from the basidiomycete Flammulina velutipes by differential display screening. Analysis of the genomic structure of fv-hyd1 revealed an open reading frame (ORF) composed of 363 nucleotides and interrupted by three introns. The deduced amino acid sequence of FV-HYD1 showed a similarity to those of other fungal class I hydrophobins and contained eight cysteine residues highly conserved among hydrophobin proteins. The pattern of the hydropathy plot of FV-HYD1 was similar to those of class I hydrophobins. Southern blot analysis of genomic DNA showed that fv-hyd1 existed as a single copy. Northern blot analysis indicated that the fv-hyd1 transcript was not present in vegetative mycelia but markedly increased in level at the primordial stage. Moreover, the fv-hyd1 transcript was abundant even at the mature fruiting body stage. This result indicates that fv-hyd1 could encode a hydrophobin closely associated with fruiting body development.
Yamada
Masato
M
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida Ueda, Nagano, 386-8567, Japan. mashimo@shinshu-u.ac.jp
Sakuraba
Sou
S
Shibata
Kou
K
Inatomi
Satoshi
S
Okazaki
Mitsuo
M
Shimosaka
Makoto
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005800:Genes, Fungal; D008969:Molecular Sequence Data
D005656:Fungal Proteins