title: In vitro effect of cyclosporine A on juvenile Schistosoma mansoni labeled by AF18 (5-N-octadecanoyl aminofluorescein).
abstract: OBJECTIVE
  To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity.
  
  
  METHODS
  Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18, then observe schistosomula under fluorescence microscope.
  
  
  RESULTS
  Schistosomula of different groups labeled by AF18 were damaged by cyclosporine A in vitro.
  
  
  CONCLUSIONS
  Cyclosporine A increases the uptake of AF18 by schistosomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.
authors.affiliation: Medical School of Ningbo University, Ningbo 315211 , China.
authors.forename: Jian-fa
authors.initials: JF
authors.lastname: Liu
authors.forename: John R
authors.initials: JR
authors.lastname: Kusel
mesh_terms: D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019793:Fluorescein / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000502:physiology
chemical_list: C116138:5-(N-octadecanoyl)aminofluorescein; D016572:Cyclosporine; D019793:Fluorescein