title: HIC1 promoter methylation and 17p13.3 allelic loss in invasive ductal carcinoma of the breast.
abstract: The HIC1 gene is a transcriptional regulator commonly methylated in a variety of human cancer. Thirty-three invasive ductal carcinomas of the breast and 21 matched normal breast tissues were analysed for HIC1 promoter methylation, and allelic loss of a 700 kb region spanning the gene locus. At least one genetic or epigenetic abnormality was found in 27 of the carcinomas tested (82%). Promoter methylation was demonstrated in 21 carcinomas (64%), and nine normal tissues (43%), whereas 18 malignant tumors (54%) showed allelic loss. Concomitant loss of heterozigosity and promoter hypermethylation in the region spanning HIC1 was detected in eight carcinomas (24%) suggesting that in this subset of tumors both copies of the gene are functionally lost. These observations support a role for the HIC1 gene in the pathogenesis of breast ductal carcinomas.
authors.affiliation: Laboratory of Gene Therapy and Oncology, IRCCS, Casa Sollievo della Sofferenza, Viale Padre Pio, 71013 San Giovanni Rotondo (FG), Italy. pparrella@operapadrepio.it
authors.forename: Paola
authors.initials: P
authors.lastname: Parrella
authors.forename: Marina
authors.initials: M
authors.lastname: Scintu
authors.forename: Maria
authors.initials: M
authors.lastname: Prencipe
authors.forename: Maria Luana
authors.initials: ML
authors.lastname: Poeta
authors.forename: Antonietta Pia
authors.initials: AP
authors.lastname: Gallo
authors.forename: Carla
authors.initials: C
authors.lastname: Rabitti
authors.forename: Monica
authors.initials: M
authors.lastname: Rinaldi
authors.forename: Stefania
authors.initials: S
authors.lastname: Tommasi
authors.forename: Angelo
authors.initials: A
authors.lastname: Paradiso
authors.forename: Francesco
authors.initials: F
authors.lastname: Schittulli
authors.forename: Vanna Maria
authors.initials: VM
authors.lastname: Valori
authors.forename: Salvatore
authors.initials: S
authors.lastname: Toma
authors.forename: Vittorio
authors.initials: V
authors.lastname: Altomare
authors.forename: Vito Michele
authors.initials: VM
authors.lastname: Fazio
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D018895:Microsatellite Repeats; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C499808:HIC1 protein, human; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors