title: The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.
abstract: The Gam1 protein of the avian CELO adenovirus activates transcription through inhibition of histone deacetylase 1 (HDAC1). We investigated the effect of Gam1 on both transient and stable transgene expression in Chinese hamster ovary (CHO) cells, one of the most commonly used mammalian hosts for the large-scale production of recombinant proteins. Transient expression of Gam1 increased reporter protein levels up to 4-fold in suspension cultures of CHO DG44 cells co-transfected with a reporter gene and up to 20-fold in recombinant CHO DG44-derived cell lines. The highest levels of activation were observed when the transgene was under the control of the human cytomegalovirus (HCMV) immediate early promoter/enhancer. Increases in recombinant protein expression in the presence of Gam1 were not accompanied by an enhancement of cell growth or viability. We conclude that Gam1 may serve as a useful genetic tool for increasing recombinant protein expression in CHO DG44 cells.
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland. david.hacker@epfl.ch
authors.forename: David L
authors.initials: DL
authors.lastname: Hacker
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Derow
authors.forename: Florian M
authors.initials: FM
authors.lastname: Wurm
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D049452:Green Fluorescent Proteins / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D019076:Transgenes; D014764:Viral Proteins / Q000494:pharmacology*
chemical_list: C105052:CELO protein, adenovirus; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins