Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis.
An electrochemiluminescence (ECL) inhibition method was developed as an indirect detection method for the determination of dopamine and epinephrine separated by capillary electrophoresis (CE). When the concentration of Ru(bpy)(3) (2+) was 50 muM diluted by 50 mM phosphate (pH 8.5) in the cell and 0.5 M tripropylamine (TPA) was added to the running buffer (10 mM phosphate, pH 9.0), an inhibition of ECL of the Ru(bpy)(3) (2+)/TPA system by the analytes was observed. Under the optimized conditions, the relative standard deviations of migration time and negative peak area were less than 1% and 3%, respectively, for 1 microM dopamine or 1 microM epinephrine (n = 10). Linear ranges of 0.1-10 microM for both analytes and the detection limits (signal-to-noise ratio S/N = 3) of 10 nM for dopamine and 30 nM for epinephrine were obtained.
Kang
Jianzhen
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. ekwang@ciac.jl.cn
Yin
Xue-Bo
XB
Yang
Xiurong
X
Wang
Erkang
E
D004298:Dopamine / Q000032:analysis*; D019075:Electrophoresis, Capillary; D004837:Epinephrine / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D011437:Propylamines / Q000737:chemistry
D009942:Organometallic Compounds; D011437:Propylamines; C415768:tris(2,2'-bipyridyl)ruthenium(II); C096226:tripropylamine; D004298:Dopamine; D004837:Epinephrine