title: [Influence of different doxorubicin-salt aggregates on the leakage of liposomal doxorubicin in vitro and long circulation in vivo].
abstract: OBJECTIVE
  To develop liposomes containing doxorubicin with different salts and to investigate their influence on the stability of liposomal doxorubicin in vitro and in vivo.
  
  
  METHODS
  Liposomes were prepared by the film method, treated further by extruded through nuclear membrane. The entrapment efficiency was determined by column chromatography. In vitro drug release experiments were carried out with a dialysis bag (Mw cut-off 12000 - 14000). Reverse-phase HPLC was used to study the pharmacokinetics of liposomal doxorubicin.
  
  
  RESULTS
  The particle size of liposomes with glycinate buffer, citrate buffer and ammonium sulfate as the inner water phase were (103 +/- 8), (102 +/- 12) and (97 +/- 8) nm. The zeta potential and the encapsulation ratio were (-21.3 +/- 0.5), (-21.7 +/- 0.4), (-20.9 +/- 0.7) mV and 47.8%, 96.7%, 98.6%, respectively. The leaking rate of doxorubicin from liposomes was related to the pH value of the release medium. The leaking rate increased at lowered pH. Pharmacokinetic study showed that the MRT (mean retention time) of liposomes with glycinate buffer, citrate buffer and ammonium sulfate as the inner water phase were 12.13, 23.31 and 29.79 h, respectively.
  
  
  CONCLUSIONS
  Doxorubicin showed different stability in liposomes with different inner water phases, the weaker the acid in the inner water phase, the stabler the liposome.
authors.affiliation: School of Pharmacy, Fudan University, Fudan-Pharmaco Targeting Drug Research Center, Shanghai 200032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Wei-yue
authors.initials: WY
authors.lastname: Lu
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D019540:Area Under Curve; D019343:Citric Acid; D004317:Doxorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004337:Drug Carriers; D004355:Drug Stability; D005998:Glycine; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008297:Male; D010316:Particle Size; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D019343:Citric Acid; D004317:Doxorubicin; D000645:Ammonium Sulfate; D005998:Glycine