title: Co-transfection of messenger RNA and siRNA as a method to study the efficiency of siRNA.
abstract: The definition of an optimal siRNA results from the in vitro testing of several siRNA designed to specifically target a gene. Usually, such in vitro tests consist in the transfection of the several siRNA duplexes in a cell expressing stably the gene of interest. When a siRNA specific for a mRNA coding toxic proteins (certain transcription factors, transporters, toxins, cell cycle controlling proteins, etc.) must be tested, the generation of a target cell is difficult. Here we report a quick method to test the efficiency of a siRNA through its co-transfection with the targeted mRNA. This technique can be used as a fast method to test siRNA even when they target genes that cannot be stably expressed in the cells of interest.
authors.affiliation: CureVac GmbH, Tübingen, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Mutzke
authors.forename: Gudrun
authors.initials: G
authors.lastname: Schubkegel
authors.forename: Regina
authors.initials: R
authors.lastname: Teufel
authors.forename: Thomas
authors.initials: T
authors.lastname: Ketterer
authors.forename: Jochen
authors.initials: J
authors.lastname: Probst
authors.forename: Birgit
authors.initials: B
authors.lastname: Scheel
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Carralot
authors.forename: Steve
authors.initials: S
authors.lastname: Pascolo
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghoreschi
authors.forename: Christina
authors.initials: C
authors.lastname: Weigert
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells; D018274:Electroporation; D015870:Gene Expression; D051379:Mice; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering