Increasing the efficiency of in-capillary electrophoretically mediated microanalysis reactions via rapid polarity switching.
We report herein a new approach to enhance the sensitivity or speed of CE-based methods that involve in-line reactions. Rapid polarity switching (RPS) is used as a novel means for in-line mixing of two reactant solutions via rapid (1-5 s) and sequential switching of the applied potential field. By employing the RPS approach with a model chemical reaction, that between creatinine and alkaline picrate, significant enhancement in sensitivity (or a decrease in analysis time) is realized. Both increased convection and electrophoretic stacking of the ionic reagent appear to contribute to the rise in apparent reaction rate. When coupled with in-line chemistry of the Jaffe method for creatinine, the RPS methodology allows for 3-fold faster determination of creatinine in the concentration range needed for the analysis of clinical blood serum specimens. The new approach also allows the analysis to be performed without the need for the cumbersome and problematic enhanced sensitivity cell.
Sanders
Brandi D
BD
Department of Chemistry, Bucknell University, Lewisburg, Pennsylvania 17837, USA.
Slotcavage
Rachel L
RL
Scheerbaum
Diana L
DL
Kochansky
Christopher J
CJ
Strein
Timothy G
TG
D003404:Creatinine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008832:Microchemistry / Q000379:methods*; D010851:Picrates / Q000737:chemistry; D012680:Sensitivity and Specificity
D010851:Picrates; D003404:Creatinine