title: Expression profiling using a tumor-specific cDNA microarray predicts the prognosis of intermediate risk neuroblastomas.
abstract: To predict the prognosis of neuroblastoma patients and choose a better therapeutic protocol, we developed a cDNA microarray carrying 5340 genes obtained from primary neuroblastomas and examined 136 tumor samples. We made a probabilistic output statistical classifier that provided a high accuracy in prognosis prediction (89% at 5 years) and a highly reliable method to validate it. Kaplan-Meier analysis indicated that the patients in an intermediate group defined by existing markers are divided by microarray into two further groups with 5 year survivals for 36% and 89% of patients (p < 10(-4)), i.e., with unfavorably and favorably predicted neuroblastomas, respectively. According to these results, we developed a gene subset chip for a clinical tool, for which our classifier exhibited 88% prediction accuracy.
authors.affiliation: Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Ohira
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Oba
authors.forename: Yohko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Eriko
authors.initials: E
authors.lastname: Isogai
authors.forename: Setsuko
authors.initials: S
authors.lastname: Kaneko
authors.forename: Atsuko
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hirata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kubo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Goto
authors.forename: Saichi
authors.initials: S
authors.lastname: Yamada
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Misa
authors.initials: M
authors.lastname: Fuchioka
authors.forename: Shin
authors.initials: S
authors.lastname: Ishii
authors.forename: Akira
authors.initials: A
authors.lastname: Nakagawara
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D020869:Gene Expression Profiling / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D015233:Models, Statistical; D009367:Neoplasm Staging; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D009447:Neuroblastoma / Q000145:classification / Q000175:diagnosis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D011379:Prognosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016019:Survival Analysis