title: SCAPE: a new tool for the Selective CApture of PEptides in protein identification.
abstract: A method for the selective capture and analysis of peptides containing neither histidine nor arginine is evaluated. It is based on the reversible modification of alpha- and epsilon-amino groups of peptides and the relatively easy separation of charged and noncharged peptides by cation exchange chromatography. The simplicity of the method and the results obtained in silico and with standard proteins, anticipate the utility of the SCAPE approach for proteome analyses.
authors.affiliation: Department for Proteome Analysis, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba. lazaro.betancourt@cigb.edu.cu
authors.forename: Lazaro
authors.initials: L
authors.lastname: Betancourt
authors.forename: Jeovanis
authors.initials: J
authors.lastname: Gil
authors.forename: Vladimir
authors.initials: V
authors.lastname: Besada
authors.forename: Luis J
authors.initials: LJ
authors.lastname: González
authors.forename: Jorge
authors.initials: J
authors.lastname: Fernández-de-Cossio
authors.forename: Liudy
authors.initials: L
authors.lastname: García
authors.forename: Rolando
authors.initials: R
authors.lastname: Pajón
authors.forename: Aniel
authors.initials: A
authors.lastname: Sanchez
authors.forename: Félix
authors.initials: F
authors.lastname: Alvarez
authors.forename: Gabriel
authors.initials: G
authors.lastname: Padrón
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011506:Proteins