Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.
To compare the structure of the precursor polypeptide of dog endothelin-3, preproendothelin-3 (PPET-3), with the PPET-3 of other mammals, we cloned dog cDNA from lung tissue using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. An open reading frame encoding a 198-amino-acid polypeptide was found in the cDNA. Regions corresponding to a bioactive mature endothelin-3 peptide, an intermediate form known as big-endothelin-3 and an endothelin-3- like peptide were observed in the putative PPET-3. Comparative analysis showed that the similarity of the dog open reading frame sequence with those from human hypothalamus, mouse intestine, and rat eye is 76.2%, 69.5% and 66.3%, respectively, and that the similarity at the amino acid level is 65.6%, 59.8% and 58.8%, respectively. RT-PCR demonstrated significant elevated expression of PPET-3 mRNA in the kidney of dog with interstitial nephritis.
Fujimori
Yuki
Y
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Uchide
Tsuyoshi
T
Temma
Kyosuke
K
Sasaki
Takushi
T
Kizaki
Keiichiro
K
Hara
Yukio
Y
Takizawa
Satoshi
S
Saida
Kaname
K
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D004285:Dogs; D019334:Endothelin-3 / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D009395:Nephritis, Interstitial / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D019334:Endothelin-3; D011498:Protein Precursors; D012333:RNA, Messenger