Direct detection of Nocardia spp. in clinical samples by a rapid molecular method.
We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.
Couble
Andrée
A
UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.
Rodríguez-Nava
Verónica
V
de Montclos
Michèle Pérouse
MP
Boiron
Patrick
P
Laurent
Frédéric
F
D001706:Biopsy; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D009615:Nocardia / Q000235:genetics / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology; D013997:Time Factors
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S