title: Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering.
abstract: In the present study, we have developed a novel and versatile method for the preparation of chitosan-peptide complex based on the selective reaction of chitosan with 2-iminothiolane. The new type of SH-chitosan derivative showed an excellent solubility to aqueous solution even in the alkaline conditions. This characteristic greatly facilitated further modification study of chitosan with a variety of bioactive substances. A synthetic peptide, RGDSGGC containing RGDS moiety that is known as one of the most important cell adhesive peptides, was readily coupled by disulfide bonds formation with sulfhydryl groups of SH-chitosan in the presence of dimethyl sulfoxide. Next, the effect of the introduction of RGDSGGC moiety to chitosan on cell adhesion and proliferation activity of chondrocytes and fibroblasts were evaluated. As a result, it was suggested that this polysaccharide-peptide conjugate exhibited excellent capacities for both cell adhesion and cell proliferation of chondrocytes and fibroblasts. Considering the growing importance of the biocompatible scaffolds in the recent tailored tissue engineering technique, these results indicate that the present strategy of 2-iminothiolane-based conjugation of polysaccharides with biologically active peptides will become a key and potential technology to develop desirable scaffold materials for the tissue regenerations.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita-Ku, Sapporo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Masuko
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yamane
authors.forename: Tadanao
authors.initials: T
authors.lastname: Funakoshi
authors.forename: Tokifumi
authors.initials: T
authors.lastname: Majima
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
authors.forename: Noriko
authors.initials: N
authors.lastname: Ohsuga
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohta
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000327:Adsorption; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology / Q000601:surgery; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000032:analysis / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology* / Q000637:transplantation; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology / Q000601:surgery; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan