Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus.
A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.
Gibertoni
Aliandra M
AM
Laboratório de Virologia e Imunologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900, Jaboticabal, Sao Paulo, Brazil.
Montassier
Maria de Fátima S
Mde F
Sena
Janete A D
JA
Givisiez
Patrícia E N
PE
Furuyama
Cibele R A G
CR
Montassier
Hélio J
HJ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D001351:Infectious bronchitis virus / Q000276:immunology*; D019590:Nucleocapsid Proteins / Q000276:immunology* / Q000378:metabolism; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins