title: Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2.
abstract: The accumulation of H2O2 by NaCl was observed in the roots of rice seedlings. Treatment with NaCl caused an increase in the activities of ascorbate peroxidase (APX) and glutathione reductase (GR) and the expression of OsAPX and OsGR in rice roots. Exogenously applied H2O2 also enhanced the activities of APX and GR and the expression of OsAPX and OsGR in rice roots. The accumulation of H2O2 in rice roots in response to NaCl was inhibited by the NADPH oxidase inhibitors, diphenyleneiodonium chloride (DPI) and imidazole (IMD). However, DPI, IMD, and dimethylthiourea, a H2O2 trap, did not reduce NaCl-enhanced activities of APX and GR and expression of OsAPX and OsGR. It appears that H2O2 is not involved in the regulation of NaCl-induced APX and GR activities and OsAPX and OsGR expression in rice roots.
authors.affiliation: Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tsai
authors.forename: Chwan-Yang
authors.initials: CY
authors.lastname: Hong
authors.forename: Li-Fei
authors.initials: LF
authors.lastname: Liu
authors.forename: Ching Huei
authors.initials: CH
authors.lastname: Kao
mesh_terms: D060387:Ascorbate Peroxidases; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005980:Glutathione Reductase / Q000187:drug effects / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D012275:Oryza / Q000201:enzymology; D010544:Peroxidases / Q000187:drug effects / Q000235:genetics*; D036226:Seedlings / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D005980:Glutathione Reductase