Effective protection of pigs against lethal Pseudorabies virus infection after a single injection of low-dose Sindbis-derived plasmids encoding PrV gB, gC and gD glycoproteins.
This study compared protection of pigs against lethal Pseudorabies virus (PrV) infection induced by a single injection of various quantities of Sindbis virus-derived plasmids encoding PrV glycoproteins gB, gC and gD. Pigs were injected with 340, 68 or 13 microg of each plasmid. Few immune differences were observed after DNA injection and more importantly the pigs of the three groups were equally protected against virulent PrV infection. Single-shot injection of 13 microg of each PrV glycoprotein encoding Sindbis virus-derived plasmid is able to effectively protect pigs from PrV infection.
Dory
Daniel
D
Viral Genetics and Biosafety Unit, Afssa, BP-53, F-22440 Ploufragan, France. d.dory@ploufragan.afssa.fr
Torché
Anne-Marie
AM
Béven
Véronique
V
Blanchard
Philippe
P
Loizel
Christophe
C
Cariolet
Roland
R
Jestin
André
A
D000818:Animals; D005822:Genetic Vectors / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage* / Q000276:immunology; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D022421:Pseudorabies Vaccines; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins