Cardiolipin synthase of Arabidopsis thaliana.
Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K(m) for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation.
Nowicki
Marcin
M
RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringerweg 1, 52056 Aachen, Germany.
Müller
Frank
F
Frentzen
Margrit
M
D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D008565:Membrane Proteins / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*
D017931:DNA Primers; D008565:Membrane Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase