title: Effect of the major repeat sequence on chromosome loss in Candida albicans.
abstract: The major repeat sequence (MRS) is found at least once on all but one chromosome in Candida albicans, but as yet it has no known relation to the phenotype. The MRS affects karyotypic variation by serving as a hot spot for chromosome translocation and by expanding and contracting internal repeats, thereby changing chromosome length. Thus, MRSs on different chromosomes and those on chromosome homologues can differ in size. We proposed that the MRS's unique repeat structure and, more specifically, the size of the MRS could also affect karyotypic variation by altering the frequency of mitotic nondisjunction. Subsequent analysis shows that both natural and artificially induced differences in the size of the chromosome 5 MRS can affect chromosome segregation. Strains with chromosome 5 homologues that differ in the size of the naturally occurring MRSs show a preferential loss of the homologue with the larger MRS on sorbose, indicating that a larger MRS leads to a higher risk of mitotic nondisjunction for that homologue. While deletion of an MRS has no deleterious effect on the deletion chromosome under normal growth conditions and leads to no obvious phenotype, strains that have the MRS deleted from one chromosome 5 homologue preferentially lose the homologue with the MRS remaining. This effect on chromosome segregation is the first demonstration of a phenotype associated with the MRS.
authors.affiliation: Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Lephart
authors.forename: Hiroji
authors.initials: H
authors.lastname: Chibana
authors.forename: Paul T
authors.initials: PT
authors.lastname: Magee
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics*; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D007621:Karyotyping; D008969:Molecular Sequence Data; D010641:Phenotype; D020079:Terminal Repeat Sequences / Q000235:genetics*
chemical_list: D004271:DNA, Fungal