Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes.
We report the use of quantum dots (QDs) to identify the presence and monitor the progression of respiratory syncytial virus (RSV) infection over time by labeling the F and G proteins. In addition, co-localization of these viral proteins was shown using confocal microscopy. The implications of these results are that QDs may provide a method for early, rapid detection of viral infection and open the door for future studies of the intricate spatial features cell trafficking of viral proteins.
Bentzen
Elizabeth L
EL
Department of Chemistry, Vanderbilt University, Station B 351822, Nashville, Tennessee 37235-1822, USA.
House
Frances
F
Utley
Thomas J
TJ
Crowe
James E
JE
Wright
David W
DW
D002460:Cell Line; D005456:Fluorescent Dyes; D006801:Humans; D045663:Quantum Dots; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000502:physiology; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000737:chemistry; D014760:Viral Fusion Proteins / Q000032:analysis* / Q000737:chemistry; D014779:Virus Replication / Q000502:physiology
D005456:Fluorescent Dyes; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C116204:attachment protein G