title: The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.
abstract: The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues.
authors.affiliation: Miyagi Prefectural Agriculture and Horticulture Research Center, Takadate-kawakami, Natori, Miyagi, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Shirasawa-Seo
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sano
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Taka
authors.initials: T
authors.lastname: Murakami
authors.forename: Yoko
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Yumi
authors.initials: Y
authors.lastname: Naito
authors.forename: Chi Ni
authors.initials: CN
authors.lastname: Hsia
authors.forename: Shigemi
authors.initials: S
authors.lastname: Seo
authors.forename: Ichiro
authors.initials: I
authors.lastname: Mitsuhara
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kosugi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D025682:Nanovirus / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D014764:Viral Proteins