cDNA cloning, sequence analysis and organ distribution of horse preproendothelin-2.
We cloned and characterized horse preproendothelin-2 (PPET-2) cDNA from intestinal tissue. The cDNA encoded 178 amino acids of the PPET-2 polypeptide, in which a 21-amino-acid mature endothelin-2 peptide and a 16-amino acid endothelin-2-like peptide were found. For the open reading frame the correspondence of horse PPET-2 cDNA with those of the ferret, human, dog, mouse and rat was 85.1%, 84.9%, 82.1%, 77.8% and 77.2%, respectively. Analysis of the organ distribution of PPET-2 mRNA by reverse transcription-polymerase chain reaction demonstrated that the kidney, stomach and small intestine are major sites of expression of the PPET-2 gene. Surprisingly, the mRNA is not detected in the large intestine, where high expression is demonstrated in the mouse and rat. This difference may result from the underlying functional differences of the large intestine between a herbivore (horse) and an omnivore (mouse and rat).
Uchide
Tsuyoshi
T
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. uchide@vmas.kitasato-u.ac.jp
Fujimori
Yuki
Y
Temma
Kyosuke
K
Sasaki
Takushi
T
Kizaki
Keiichiro
K
Hara
Yukio
Y
Takizawa
Satoshi
S
Saida
Kaname
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004285:Dogs; D019333:Endothelin-2 / Q000737:chemistry / Q000235:genetics*; D005289:Ferrets; D006736:Horses; D006801:Humans; D007421:Intestine, Small / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013270:Stomach / Q000737:chemistry
D019333:Endothelin-2; D011498:Protein Precursors; D012333:RNA, Messenger