Rapid immunofluorescence microscopy for diagnosis of melioidosis.
An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.
Wuthiekanun
Vanaporn
V
Faculty of Tropical Medicine, Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program, Bangkok, Thailand. lek@tropmedres.ac
Desakorn
Varunee
V
Wongsuvan
Gumphol
G
Amornchai
Premjit
P
Cheng
Allen C
AC
Maharjan
Bina
B
Limmathurotsakul
Direk
D
Chierakul
Wirongrong
W
White
Nicholas J
NJ
Day
Nicholas P J
NP
Peacock
Sharon J
SJ
D000942:Antigens, Bacterial / Q000097:blood*; D001431:Bacteriological Techniques; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial