title: Analysis of cellular fatty acids in Orientia tsutsugamushi as taxonomic markers.
abstract: Six Orientia strains including 3 prototype strains such as Gilliam, Karp, and Kato, and 3 strains (Boryong, Pajoo, and Yongworl) isolated in Korea, were studied for the profiles of their cellular fatty acids. All tested strains contained octadecenoic acid C (18: 1) omega 9 c(57.3 +/- 3.5%), octadecanoic acid C (18: 0) (15.3 +/- 1.5%), and hexadecanoic acid C (16: 0) (12.7 +/- 1.7%) as major components; however, interestingly, eicosenoic acid C (20: 1) omega 9 c(2.6 +/- 0.6%) was found in all strains except the Yongworl strain. Furthermore none of the strains contained 3-hydroxy fatty acids. The ratios of total saturated fatty acid (SFA) to total unsaturated fatty acid (UFA) were within the range of 0.34 to 0.54. These results showed that the cellular fatty acid profile should provide more reliable information for the identification of these bacteria.
authors.affiliation: Division of Rickettsial and Zoonotic Diseases, Department of Bacteriology, National Institute of Health, Seoul, Korea.
authors.forename: Soo-Kyoung
authors.initials: SK
authors.lastname: Shim
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Shin
authors.forename: Eun-Na
authors.initials: EN
authors.lastname: Choi
authors.forename: Bok-Ghee
authors.initials: BG
authors.lastname: Han
authors.forename: Hae-Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Young-Sill
authors.initials: YS
authors.lastname: Choi
authors.forename: Byung-Chul
authors.initials: BC
authors.lastname: Lee
authors.forename: Kwon-Sang
authors.initials: KS
authors.lastname: Yoon
authors.forename: Mi-Yeoun
authors.initials: MY
authors.lastname: Park
mesh_terms: D015415:Biomarkers; D004537:Eicosanoic Acids / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D007723:Korea; D009829:Oleic Acids / Q000032:analysis / Q000737:chemistry; D012285:Orientia tsutsugamushi / Q000737:chemistry* / Q000145:classification*; D019308:Palmitic Acid / Q000032:analysis / Q000737:chemistry
chemical_list: D015415:Biomarkers; D004537:Eicosanoic Acids; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D019308:Palmitic Acid