[Immunoglobulin isotype and IgG subclass profiles in American tegumentary leishmaniasis].
The present work investigated the serum antibody profiles in 37 patients with American tegumentary leishmaniasis, who were attended at Hospital de Clinicas - Universidade Federal de Uberlandia, MG, Brazil. The immunoglobulin class and IgG subclass profiles were analyzed by indirect ELISA using Leishmania (Leishmania) amazonensis soluble antigen. The antibody avidity was determined by 6 M urea treatment after incubation with immunoenzymatic conjugate. It was observed that 97% of the serum samples presented anti-Leishmania antibodies for IgE class, 94.6% IgG, 57.5% IgA and 21.5% IgM class. For IgG subclasses the profiles were in the following order of frequency: IgG1>IgG3>IgG2>IgG4. High avidity of anti-Leishmania IgE antibodies was found in 44.4% of the samples. On the other hand, moderate avidity of specific IgG and IgA was observed in 62.8% and 47.8% of samples, respectively. These results indicate a very complex antibody response profile against American tegumentary leishmaniasis.
de Souza
Maria Aparecida
MA
Laboratório de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
da Silva
Adriano Gomes
AG
Afonso-Cardoso
Sandra Regina
SR
Favoreto
Silvio Junior
SJ
Ferreira
Marcelo Simão
MS
D000328:Adult; D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000915:Antibody Affinity; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology*; D007136:Immunoglobulins / Q000097:blood* / Q000276:immunology; D007892:Leishmania braziliensis / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000913:Antibodies, Protozoan; D015415:Biomarkers; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins