SPOC1, a novel PHD-finger protein: association with residual disease and survival in ovarian cancer.
We report the identification of a novel human gene (SPOC1) which encodes a protein with a PHD-finger domain. The gene is located in chromosomal region 1p36.23, a region implicated in tumor development and progression. RNA in situ hybridization experiments showed strong SPOC1 expression in some rapidly proliferating cell types, such as spermatogonia, but not in nonproliferating mature spermatocytes. In addition, high SPOC1 mRNA expression was observed in several ovarian cancer cell lines. This prompted us to systematically examine SPOC1 expression in ovarian cancer in relation to prognosis. SPOC1 mRNA expression was quantified in tumor tissue of 103 patients with epithelial ovarian cancer. Interestingly, SPOC1 was associated with residual disease, whereby patients with unresectable tumors showed higher levels compared to patients without residual tumor tissue after surgery (p = 0.029). The univariable proportional hazards model showed an association between SPOC1 expression and survival (p = 0.043, relative risk = 1.535). Median survival time was 1,596 days for patients with low SPOC1 expression vs. only 347 days for patients with high expression, using Kaplan-Meier analysis. However, SPOC1 was not associated with survival when multivariable analysis was adjusted for residual disease. This can be explained by the correlation between residual disease and SPOC1 expression. In conclusion, SPOC1 is a novel PHD-finger protein showing strong expression in spermatogonia and ovarian cancer cells. SPOC1 overexpression was associated with unresectable carcinomas and shorter survival in ovarian cancer.
Mohrmann
Gerrit
G
Institute of Human Genetics, University of Erlangen-Nuremberg, Erlangen, Germany.
Hengstler
Jan G
JG
Hofmann
Thomas G
TG
Endele
Sabine U
SU
Lee
Brendan
B
Stelzer
Christiane
C
Zabel
Bernhard
B
Brieger
Juergen
J
Hasenclever
Dirk
D
Tanner
Berno
B
Sagemueller
Jens
J
Sehouli
Jalid
J
Will
Hans
H
Winterpacht
Andreas
A
D000368:Aged; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013093:Spermatogonia / Q000502:physiology; D016019:Survival Analysis
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D011509:Proteoglycans; D012333:RNA, Messenger