Dietary n-3 polyunsaturated fatty acids increase T-lymphocyte phospholipid mass and acyl-CoA binding protein expression.
Dietary flaxseed oil, which is enriched in alpha-linolenic acid, and fish oil, which is enriched in EPA and DHA, possess anti-inflammatory properties when compared with safflower oil, which is enriched in linoleic acid. The influence of flaxseed oil and fish oil feeding on lipid metabolism in T-lymphocytes is currently unknown. This study directly compared the effects of feeding safflower oil, flaxseed oil, and fish oil for 8 wk on splenic T-lymphocyte proliferation, phospholipid mass, and acyl-CoA binding protein expression in the rat. The data show that both flaxseed oil and fish oil increased acyl-CoA binding protein expression and phosphatidic acid mass in unstimulated T-lymphocytes when compared with safflower oil feeding. Fish oil feeding increased cardiolipin mass, whereas flaxseed oil had no effect. After stimulation, flaxseed oil and fish oil blunted T-lymphocyte interleukin-2 production and subsequent proliferation, which was associated with the lack of increased acyl-CoA binding protein expression. The results reported show evidence for a novel mechanism by which dietary flaxseed oil and fish oil suppress T-lymphocyte proliferation via changes in acyl-CoA binding protein expression and phospholipid mass.
Collison
Lauren W
LW
Division of Nutritional Sciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Collison
Robert E
RE
Murphy
Eric J
EJ
Jolly
Christopher A
CA
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D026261:Diazepam Binding Inhibitor / Q000032:analysis*; D004042:Dietary Fats, Unsaturated / Q000276:immunology / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000276:immunology / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D008043:Linseed Oil / Q000494:pharmacology; D008297:Male; D010743:Phospholipids / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012450:Safflower Oil / Q000494:pharmacology; D013601:T-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D026261:Diazepam Binding Inhibitor; D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010743:Phospholipids; D012450:Safflower Oil; D008043:Linseed Oil