[Spectrophotometric assay of polyethyene glycol in urine using ammonium ferrothiocyanate aqueous-chloroform system].
A spectrophotometric method for the quantitative determination of polyethylene glycol-600 (PEG-600) in urine is based on partitioning a chromophore present in ammonium ferrothiocyanate reagent into an aqueous phase and a chloroform phase in the presence of PEG. The method is simple and reproducible, and can detect PEG in a concentration of 0.25 g/L. It gives a linear response over a range of 0.25-2.5 g/L. The recovery of PEG-600 in urine is 107% and relative standard deviation is less than 5%.
Han
R
R
School of Chemistry and Chemical Engineering, Zhengzhou University, 450052 Zhengzhou.
Yang
G
G
Yin
J
J
Wu
X
X
Guan
D
D
D002725:Chloroform / Q000737:chemistry; D006801:Humans; D011092:Polyethylene Glycols / Q000032:analysis*; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D013861:Thiocyanates / Q000737:chemistry; D014556:Urine / Q000737:chemistry*
D013861:Thiocyanates; C025244:ammonium ferrothiocyanate; D011092:Polyethylene Glycols; D002725:Chloroform