Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout (Oncorhynchus mykiss).
BACKGROUND
Comparative genomics, through the integration of genetic maps from species of interest with whole genome sequences of other species, will facilitate the identification of genes affecting phenotypes of interest. The development of microsatellite markers from expressed sequence tags will serve to increase marker densities on current salmonid genetic maps and initiate in silico comparative maps with species whose genomes have been fully sequenced.


RESULTS
Eighty-nine polymorphic microsatellite markers were generated for rainbow trout of which at least 74 amplify in other salmonids. Fifty-five have been associated with functional annotation and 30 were mapped on existing genetic maps. Homologous sequences were identified for 20 of the EST containing microsatellites to identify comparative assignments within the tetraodon, mouse, and/or human genomes.


CONCLUSIONS
The addition of microsatellite markers constructed from expressed sequence tag data will facilitate the development of high-density genetic maps for rainbow trout and comparative maps with other salmonids and better studied species.
Rexroad
Caird E
CE
USDA/ARS National Center for Cool and Cold Water Aquaculture, Kearneysville, West Virginia 25430, USA. crexroad@ncccwa.ars.usda.gov
Rodriguez
Maria F
MF
Coulibaly
Issa
I
Gharbi
Karim
K
Danzmann
Roy G
RG
Dekoning
Jenefer
J
Phillips
Ruth
R
Palti
Yniv
Y
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D016678:Genome; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D005819:Genetic Markers