title: Oceanimonas smirnovii sp. nov., a novel organism isolated from the Black Sea.
abstract: A slightly creamy, melanogenic, gram-negative, aerobic bacterium was isolated from seawater sample collected in the Karadag Natural Reserve of the Eastern Crimea, the Black Sea. The novel organism was chemoorganotrophic, had no obligate requirement in NaCl, tolerated to 12% NaCl, grew between 10 and 45 degrees C, was slightly alkaliphilic, and was not able to degrade starch, gelatin, agar, and Tween 80. 16S rRNA gene sequence-based analyses of the new organism revealed that Oceanimonas doudoroffii ATCC 27123T, Oceanimonas baumanii ATCC 700832T, and Oceanisphaera litoralis DSM 15406T were the closest relatives (similarity around 97%-96%). The G + C content of the DNA of the strain 31-13T was 55.5mol%. Phosphatidylethanolamine (49.0%), phosphatidylglycerol (41.8%), and diphosphatidylglycerol (9.2%) were the predominant phospholipids. The major fatty acids were 16:0 (24.1%), 16:1omega7 (40.3%), and 18:1omega7 (29.2%). On the basis of the significant differences demonstrated in the phenotypic and chemotaxonomic characteristics, it is suggested that the bacterium be classified as a novel species; the name Oceanimonas smirnovii sp. nov. is proposed. The type strain is 31-13T (UCM B-11076T = LMG 22147T = ATCC BAA-899T).
authors.affiliation: Swinburne University of Technology, P.O. Box 218, Hawthorn, Vic. 3122, Australia. eivanova@swin.edu.au
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.forename: Olga M
authors.initials: OM
authors.lastname: Onyshchenko
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.forename: Anatoly M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: Ludmila S
authors.initials: LS
authors.lastname: Shevchenko
authors.forename: Vlado
authors.initials: V
authors.lastname: Buljan
authors.forename: Brett
authors.initials: B
authors.lastname: Hambly
authors.forename: Elena A
authors.initials: EA
authors.lastname: Kiprianova
mesh_terms: D044044:Aeromonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014455:Ukraine
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S