Elevation of plasma and cerebrospinal fluid osteopontin levels in patients with atypical teratoid/rhabdoid tumor.
Osteopontin, a cancer metastasis-associated gene, is specifically up-regulated in central nervous system (CNS) atypical teratoid/rhabdoid tumor (AT/RT), but its biological behavior in the progression of CNS AT/RT has never been studied. We obtained plasma, cerebrospinal fluid (CSF), and brain tissue specimens from lobectomy or hemispherectomy samples from 39 patients (medulloblastoma, 16; AT/RT, 8; epilepsy, 6; hydrocephalus, 9). By enzyme-linked immunosorbent assay, the median osteopontin levels in plasma and CSF in AT/RT (852.0 and 1,175.0 ng/mL, respectively) were significantly higher than in medulloblastoma (492.5 and 524.5 ng/mL, respectively) and hydrocephalus and epilepsy (208.0 and 168.0 ng/mL, respectively) (P < .05). The results of real-time reverse transcriptase-polymerase chain reaction and immunohistochemical analysis demonstrated that osteopontin expression in AT/RT (n = 5) was significantly higher than in medulloblastoma (n = 8) samples. The differences in osteopontin expression in plasma, CSF, and tumor samples in AT/RT and medulloblastoma correlated with survival differences. In 5 patients with AT/RT, plasma osteopontin levels decreased after treatment but increased with relapse. Osteopontin might be a potential marker to aid in identifying AT/RT recurrence.
Kao
Chung-Lan
CL
Department of Physical Medicine and Rehabilitation, the Neurological Institute, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan.
Chiou
Shih-Hwa
SH
Ho
Donald Ming-Tak
DM
Chen
Yann-Jang
YJ
Liu
Ren-Shyan
RS
Lo
Chih-Wen
CW
Tsai
Fu-Ting
FT
Lin
Chi-Hung
CH
Ku
Hung-Hai
HH
Yu
Shang-Ming
SM
Wong
Tai-Tong
TT
D000293:Adolescent; D000328:Adult; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008527:Medulloblastoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D009364:Neoplasm Recurrence, Local; D053495:Osteopontin; D018335:Rhabdoid Tumor / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D012795:Sialoglycoproteins / Q000097:blood / Q000134:cerebrospinal fluid; D015996:Survival Rate; D013724:Teratoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin