title: Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh.
abstract: In previous studies with strains of the Shigella dysenteriae provisional serovars E22383 and E23507 from diarrhoeal stools from patients in Bangladesh, two strains of Shigella species were identified as Shigella boydii provisional serovar E16553 by a reference laboratory. Further tests with an antiserum to an international type strain of the provisional serovar E16553 identified an additional 15 isolates. None of the isolates reacted with antisera to the established Shigella serovars or any other provisional serovars reported so far and all showed biochemical reactions typical of S. boydii. All of the isolates harboured the 140 MDa invasion plasmid, had the ipaH gene and produced keratoconjunctivitis in the guinea pig eye. All isolates were susceptible to ampicillin, sulfamethoxazole-trimethoprim, nalidixic acid, ciprofloxacin and mecillinam but eight strains were resistant to tetracycline. A single PFGE type (type A) was shown for all 17 clinical isolates, indicating a common source of origin. The pulsotype of the Bangladeshi isolates was closely related to that of a Japanese strain but was different from that of the type strain. On the basis of these biochemical, serological and virulence markers, and diverse geographical origin, it is recommended that the provisional status of serovar E16553 be changed and that it be included in the international serotyping classification scheme as S. boydii 19.
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: M
authors.initials: M
authors.lastname: Ansaruzzaman
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Marzia
authors.initials: M
authors.lastname: Sultana
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Kaisar A
authors.initials: KA
authors.lastname: Talukder
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: K
authors.initials: K
authors.lastname: Alam
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: S
authors.initials: S
authors.lastname: Matsushita
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Ashrafus
authors.initials: A
authors.lastname: Safa
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Bijay K
authors.initials: BK
authors.lastname: Khajanchi
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Dutta
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Zhahirul
authors.initials: Z
authors.lastname: Islam
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: M John
authors.initials: MJ
authors.lastname: Albert
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001459:Bangladesh; D004352:Drug Resistance, Microbial; D004405:Dysentery, Bacillary / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D007637:Keratoconjunctivitis / Q000382:microbiology; D012761:Shigella boydii / Q000145:classification* / Q000472:pathogenicity / Q000502:physiology; D012762:Shigella dysenteriae / Q000145:classification; D014774:Virulence
chemical_list: D000890:Anti-Infective Agents