title: Flow cytometry: a new method to investigate the properties of water-in-oil-in-water emulsions.
abstract: We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.
authors.affiliation: Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Givat Ram, 91904 Jerusalem, Israel.
authors.forename: Mingtan
authors.initials: M
authors.lastname: Hai
authors.forename: Kalia
authors.initials: K
authors.lastname: Bernath
authors.forename: Dan
authors.initials: D
authors.lastname: Tawfik
authors.forename: Shlomo
authors.initials: S
authors.lastname: Magdassi