Pilot Anopheles gambiae full-length cDNA study: sequencing and initial characterization of 35,575 clones.
We describe the preliminary analysis of over 35,000 clones from a full-length enriched cDNA library from the malaria mosquito vector Anopheles gambiae. The clones define nearly 3,700 genes, of which around 2,600 significantly improve current gene definitions. An additional 17% of the genes were not previously annotated, suggesting that an equal percentage may be missing from the current Anopheles genome annotation.
Gomez
Shawn M
SM
Unité de Biochimie et Biologie Moléculaire des Insectes and CNRS FRE 2849, Institut Pasteur, 75724 Paris Cedex 15, France. sgomez@pasteur.fr
Eiglmeier
Karin
K
Segurens
Beatrice
B
Dehoux
Pierre
P
Couloux
Arnaud
A
Scarpelli
Claude
C
Wincker
Patrick
P
Weissenbach
Jean
J
Brey
Paul T
PT
Roth
Charles W
CW
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001482:Base Composition / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010865:Pilot Projects; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D010457:Peptidoglycan