title: Genetic heterogeneity of the tropical abalone (Haliotis asinina) revealed by RAPD and microsatellite analyses.
abstract: Genetic heterogeneity of the tropical abalone, Haliotis asinina was examined using randomly amplified polymorphic DNA (RAPD) and microsatellite analyses. One hundred and thirteen polymorphic RAPD fragments were generated. The percentage of polymorphic bands of H. asinina across overall primers was 85.20%. The average genetic distance of natural samples within the Gulf of Thailand (HACAME and HASAME) was 0.0219. Larger distance was observed when those samples were compared with HATRAW from the Andaman Sea (0.2309 and 0.2314). Geographic heterogeneity and F(ST) analyses revealed population differentiation between H. asinina from the Gulf of Thailand and the Andaman Sea (p < 0.0001). Three microsatellite loci (CUHas1, CUHas4 and CUHas5) indicated relatively high genetic diversity in H. asinina (total number of alleles = 26, 5, 23 and observed heterozygosity = 0.84, 0.42 and 0.33, respectively). Significant population differentiation was also found between samples from different coastal regions (p < 0.0001). Therefore, the gene pool of natural H. asinina in coastal Thai waters can be genetically divided to 2 different populations; the Gulf of Thailand (A) and the Andaman Sea (B).
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Sureerat
authors.initials: S
authors.lastname: Tang
authors.forename: Aporn
authors.initials: A
authors.lastname: Popongviwat
authors.forename: Sirawut
authors.initials: S
authors.lastname: Klinbunga
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
authors.forename: Padermsak
authors.initials: P
authors.lastname: Jarayabhand
authors.forename: Piamsak
authors.initials: P
authors.lastname: Menasveta
mesh_terms: D000818:Animals; D005787:Gene Frequency; D018740:Genetic Heterogeneity; D018895:Microsatellite Repeats; D008974:Mollusca / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013785:Thailand