Soybean DRE-binding transcription factors that are responsive to abiotic stresses.
Three DREB homologue genes, GmDREBa, GmDREBb, and GmDREBc, were isolated from soybean, Glycine max (L.) Merr. Each of the deduced proteins contains an AP2 domain of 64 amino acids. Yeast one-hybrid assay revealed that all of the three dehydration-responsive, element-binding proteins specifically bound to the dehydration-responsive element. Analysis of transcriptional activation abilities of these proteins in yeast indicated that GmDREBa and GmDREBb could activate the expression of a reporter gene, whereas GmDREBc could not. The transcriptions of GmDREBa and GmDREBb were induced by salt, drought, and cold stresses in leaves of soybean seedlings. The expression of GmDREBc was not significantly affected in leaves but apparently induced in roots by salt, drought, and abscisic acid treatments. These results suggest that these three genes function specifically in response to abiotic stresses in soybean.
Li
Xue-Ping
XP
National Key Laboratory of Plant Genomic, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing, 100101, China.
Tian
Ai-Guo
AG
Luo
Guang-Zuo
GZ
Gong
Zhi-Zhong
ZZ
Zhang
Jin-Song
JS
Chen
Shou-Yi
SY
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers; D003681:Dehydration / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D017931:DNA Primers; D014157:Transcription Factors