Effects of dendritic cells from cord blood CD34+ cells on human hepatocarcinoma cell line BEL-7402 in vitro and in SCID mice.
OBJECTIVE
To develop a cancer vaccine of dendritic cells derived from human cord blood CD34+ cells and to investigate its cytotoxicity on human hepatocarcinoma cells in vitro and in severe combined immunodeficiency (SCID) mice.


METHODS
Lymphocytes from cord blood or peripheral blood were primed by DCs, which were derived from cord blood and pulsed with whole tumor cell lysates. Nonradiative neutral red uptake assay was adopted to detect the cytotoxicity of primed lymphocytes on human hepatocarcinoma cell line BEL-7402 in vitro. The anti-tumor effect of primed lymphocytes in vivo was detected in SCID mice, including therapeutic effect and vaccination effect.


RESULTS
The cytotoxicity of DC vaccine primed lymphocytes from cord blood or peripheral blood on human hepatocarcinoma cell line BEL-7402 was significantly higher than that of unprimed lymphocytes in vitro (44.09% vs 14.69%, 47.92% vs 19.44%, P<0.01). There was no significant difference between the cytotoxicity of primed lymphocytes from cord blood and peripheral blood (P>0.05). The tumor growth rate and tumor size were smaller in SCID mice treated or vaccinated with primed lymphocytes than those with unprimed lymphocytes. SCID mice vaccinated with primed lymphocytes had a lower tumor incidence (80% vs 100%, P<0.05) and delayed tumor latent period compared with mice vaccinated with unprimed lymphocytes (11 d vs 7 d, P<0.01).


CONCLUSIONS
Vaccine of cord blood derived-DCs has an inhibitory activity on growth of human hepatocarcinoma cells in vitro and in SCID mice. The results also implicate the potential role of cord blood derived-DC vaccine in clinical tumor immunotherapy.
Su
Zhong-Jing
ZJ
Cancer Pathology Laboratory, Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, Guangdong Province, China.
Chen
Hai-Bin
HB
Zhang
Jin-Kun
JK
Xu
Lan
L
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D019496:Cancer Vaccines / Q000276:immunology / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000628:therapy; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology* / Q000628:therapy; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D023041:Xenograft Model Antitumor Assays
D018952:Antigens, CD34; D019496:Cancer Vaccines