[Effects of basic fibroblast growth factor on protein kinase B activity and c-fos expression in CNE- I nasopharyngeal carcinoma cell line].
OBJECTIVE
The relationship between basic fibroblast growth factor (bFGF) and phosphatidylinositol 3-kinase/protein kinase B(PI 3-K/PKB) signal pathway was studied during the course of bFGF regulating carcinoma epithelioid cell lines- I (CNE- I ) nasopharyngeal carcinoma cell proliferation through altering time of bFGF treatment.


METHODS
PKB activities were measured by advanced Takai method. C-fos expression was examined by Western Blot.


RESULTS
bFGF (50 ng/ml) significantly stimulated PKB activity,After pretreatment of Wortmannin for 1 h, the PKB activity decreases to the basal levels(P < 0. 05),compared with control group. After treatment of bFGF (50 ng/ml) at various time, the expression of c-fos is increased. The expression of c-fos increases to the peak when the time of bFGF treatment is 30 min. After pretreatment of Wortmannin for 1 h, the expression of c-fos decreases 20% when the time of bFGF treatment was 30 min and when the time of bFGF treatment was 45 min, the expression of c-fos decreases 35%.


CONCLUSIONS
1. PI 3-K and PKB mediate bFGF-induced signal transduction in CNE- I nasopharyngeal carcinoma cell line; PKB lies in downstream of PI 3-K. 2. bFGF induces the increased expression of c-fos through PI 3-K/PKB in CNE- I nasopharyngeal carcinoma cell line.
Wang
Xiao-peng
XP
Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
Sun
Li-guang
LG
Liu
Ning
N
Yu
Hui-ying
HY
Zhang
Yi
Y
Shan
Yong-qiang
YQ
D002277:Carcinoma; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D016760:Proto-Oncogene Proteins c-fos; D016222:Fibroblast Growth Factor 2; D051057:Proto-Oncogene Proteins c-akt