title: Low molecular weight fraction of shark cartilage can modulate immune responses and abolish angiogenesis.
abstract: Shark cartilage has proven to have inhibitory effects on angiogenesis. In this research, we studied the effects of shark cartilage on the immune system. Firstly, we isolated and purified a shark cartilage protein fraction with the most immunostimulatory effects. Our fraction was composed of two proteins with molecular weights of about 14 and 15 kDa. This fraction highly augments delayed-type hypersensitivity response against sRBC in mice, and decreases the cytotoxic activity of Natural Killer cells. Furthermore, intraperitoneal injection of this fraction to tumor-bearing mice could increase T-cell infiltration into the tumor, and decrease the tumor lesion size. Also, this fraction has strong inhibitory effect on HBMEC proliferation and migration in fibrin matrix. According to these results, we suppose that this fraction is a good candidate for further studies in cancer therapy.
authors.affiliation: Department of Immunology, School of Medical Sciences, Tarbiat Modarres University, P.O. Box: 14115-111, Tehran, IR Iran. hasan_zm@modares.ac.ir
authors.forename: Zuhair M
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Reza
authors.initials: R
authors.lastname: Feyzi
authors.forename: Ali
authors.initials: A
authors.lastname: Sheikhian
authors.forename: Afshar
authors.initials: A
authors.lastname: Bargahi
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafaie
authors.forename: Kamran
authors.initials: K
authors.lastname: Mansouri
authors.forename: Somayeh
authors.initials: S
authors.lastname: Shahrokhi
authors.forename: Tooba
authors.initials: T
authors.lastname: Ghazanfari
authors.forename: Shahram
authors.initials: S
authors.lastname: Shahabi
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D016516:CD4-CD8 Ratio; D002356:Cartilage / Q000737:chemistry*; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000502:physiology; D004912:Erythrocytes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012754:Sharks / Q000378:metabolism*; D012756:Sheep / Q000276:immunology; D014020:Tissue Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D020533:Angiogenesis Inhibitors; D007155:Immunologic Factors; D014020:Tissue Extracts