[Inhibition of HBV core antigen gene expression in human embryonic kidney cell line AD293 by plasmid-based RNAi].
To inhibit HBV core antigen gene expression with plasmid-based RNAi.



The shRNA expression vector targeting HBV core antigen gene was designed and constructed. Human embryonic kidney cell line AD293 was co-transfected with HBcAg-EGFP fusion protein expression vector and shRNA expression vector transiently, and the cells without shRNA-transfection and with non-specific shRNA transfection were used as controls. Inhibitory effect of RNAi was detected by fluorescence-activated cell sorting (FACS) and real-time fluorescence quantificational RT-PCR.



HBV core antigen gene expression in AD293 was inhibited by shRNA, with the maximal inhibition rate of 76 % measured by FACS and of 63.1 % by real-time PCR.



Effective inhibition of HBV core antigen gene expression by plasmid-based RNAi provides an alternative for anti-HBV study in vitro, which has potential clinical application.
Xu
Ning
N
Institute of Infectious Diseases, The First Affiliated Hospital, Key Laboratory of Infectious Diseases Ministry of Health, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Yang
Zheng-gang
ZG
Zhu
Hai-hong
HH
Yao
Hang-ping
HP
Hou
Xiao-li
XL
Wu
Wei
W
D002460:Cell Line; D004622:Embryo, Mammalian; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D034622:RNA Interference / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D006512:Hepatitis B Core Antigens; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins