title: [Effects of basic fibroblast growth factor on protein kinase B activity and c-fos expression in CNE- I nasopharyngeal carcinoma cell line].
abstract: OBJECTIVE
  The relationship between basic fibroblast growth factor (bFGF) and phosphatidylinositol 3-kinase/protein kinase B(PI 3-K/PKB) signal pathway was studied during the course of bFGF regulating carcinoma epithelioid cell lines- I (CNE- I ) nasopharyngeal carcinoma cell proliferation through altering time of bFGF treatment.
  
  
  METHODS
  PKB activities were measured by advanced Takai method. C-fos expression was examined by Western Blot.
  
  
  RESULTS
  bFGF (50 ng/ml) significantly stimulated PKB activity,After pretreatment of Wortmannin for 1 h, the PKB activity decreases to the basal levels(P < 0. 05),compared with control group. After treatment of bFGF (50 ng/ml) at various time, the expression of c-fos is increased. The expression of c-fos increases to the peak when the time of bFGF treatment is 30 min. After pretreatment of Wortmannin for 1 h, the expression of c-fos decreases 20% when the time of bFGF treatment was 30 min and when the time of bFGF treatment was 45 min, the expression of c-fos decreases 35%.
  
  
  CONCLUSIONS
  1. PI 3-K and PKB mediate bFGF-induced signal transduction in CNE- I nasopharyngeal carcinoma cell line; PKB lies in downstream of PI 3-K. 2. bFGF induces the increased expression of c-fos through PI 3-K/PKB in CNE- I nasopharyngeal carcinoma cell line.
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China.
authors.forename: Xiao-peng
authors.initials: XP
authors.lastname: Wang
authors.forename: Li-guang
authors.initials: LG
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Yu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Shan
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016222:Fibroblast Growth Factor 2; D051057:Proto-Oncogene Proteins c-akt