title: High-level aminoglycoside resistant enterococci isolated from swine.
abstract: Approximately 42% (187/444) of swine enterococci collected between the years 1999 and 2000 exhibited high-level resistance to gentamicin (MIC > or =500 microg/ml), kanamycin (MIC > or =500 microg/ml), or streptomycin (MIC > or =1000 microg/ml). Eight aminoglycoside resistance genes were detected using PCR, most frequently ant(6)-Ia and aac(6')-Ii from Enterococcus faecium. Twenty-four per cent (45/187) of total high-level aminoglycoside-resistant isolates and 26% (4/15) of isolates resistant to high levels of all three antimicrobials were negative for all genes tested. These data suggest that enterococci isolated from swine contain diverse and possibly unidentified aminoglycoside resistance genes.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Antimicrobial Resistance Research Unit, USA. cjackson@saa.ars.usda.gov
authors.forename: C R
authors.initials: CR
authors.lastname: Jackson
authors.forename: P J
authors.initials: PJ
authors.lastname: Fedorka-Cray
authors.forename: J B
authors.initials: JB
authors.lastname: Barrett
authors.forename: S R
authors.initials: SR
authors.lastname: Ladely
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D016908:Gram-Positive Bacterial Infections; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial