[Effect of intranasal glucosteroid on the gene expression of interleukin-5 in nasal polyps].
OBJECTIVE
To investigate the effect of intranasal glucosteroid treatment on the expression of interleukin (IL)-5 gene in nasal polyps.


METHODS
Nasal polyps from topical steroid treated patients (n = 20) and untreated patients (n = 20) were investigated with the technique of mRNA in situ hybridization.


RESULTS
The majority of IL-5 mRNA positive cells in nasal polyps were lymphocytes or eosinophils. No statistical significance was found in the densities of IL-5 mRNA positive cells between allergic patients [(12.6 +/- 4.6)/0.25mm2] and nonallergic patients [(14.3 +/- 4.1)/0.25mm2] (t = -0.775, P > 0.05). Compared with the control group [(13.9 +/- 4.2)/0.25mm2], the density of IL-5 mRNA positive cells was decreased in the steroid-treated group [(10. 2 +/- 3.1)/0.25mm2], and the difference reached statistical significance (t = 3.114, P < 0.01).


CONCLUSIONS
These findings indicate that topical steroid treatment may suppress the IL-5 gene expression, and steroids may inhibit eosinophil functions.
Zhang
Luo
L
Beijing Institute of Otorhinolaryngology, Department of Otorhinolaryngology, Head and Neck Surgery, Affiliated Beijing Tongren Hospital of Capital University of Medical Sciences, Beijing, China. luozhang@trhos.com
Han
De-min
DM
Zhou
Bing
B
Fan
Er-zhong
EZ
Liu
Zhong-yan
ZY
D000281:Administration, Intranasal; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000627:therapeutic use; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009298:Nasal Polyps / Q000188:drug therapy* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D005938:Glucocorticoids; C508599:IL5 protein, human; D015848:Interleukin-5; D012333:RNA, Messenger