title: [Determination of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma].
abstract: OBJECTIVE
  To establish a method by high-performance liquid chromatography (HPLC) for determining the concentration of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma.
  
  
  METHODS
  Chromatography was performed on a LiChroCART C18 (250 mm x 4 mm, 5 microm) column with the mobile phase consisting of acetonitrile-NaAC (15:85), the flow rate of 1.0 ml/min, and the detection wavelength of 365 nm. Sample extraction was carried out with ethylacetate.
  
  
  RESULTS
  The linear range of mouse plasma mitomycin C concentration was 0.04-1.00 microg/ml, and the linear equation of Y=16 388X-17.17 (r=0.999 8) was derived.
  
  
  CONCLUSIONS
  This method is very easy to operate and suits the need of perclinical pharmacokinetic studies of mitomycin-magnetic nanoparticles and yields accurate and precise results.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lw0913@fimmu.com
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jian-Hai
authors.initials: JH
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Guo
authors.forename: Xi-Qing
authors.initials: XQ
authors.lastname: Yan
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004659:Enbucrilate; D008280:Magnetics; D051379:Mice; D016685:Mitomycin / Q000008:administration & dosage / Q000097:blood*; D053758:Nanoparticles
chemical_list: D004337:Drug Carriers; D016685:Mitomycin; D004659:Enbucrilate