title: Protection by food-derived antioxidants from UV-A1-induced photodamage, measured using living skin equivalents.
abstract: In a study of biomarkers of ultraviolet-A1 radiation (UV-A1)-induced skin damage, living skin equivalent cultures (LSE) were treated with the antioxidants hesperetin and quercetin-3-glucoside and irradiated with 25 or 50 J/cm2 UV-A1. Changes in the following biomarkers were measured; Interleukin 1-alpha (IL-1alpha), Heme Oxygenase-1 (HO-1), TdT-mediated dUTP nick end labeling (TUNEL) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). IL-1alpha and HO-1 were analyzed by real-time PCR, Western blot, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. TUNEL and 8-OHdG were determined by (immuno)histochemical techniques. Sections were stained with hematoxylin and eosin (H&E). UV-A1 induced keratinocyte and fibroblast vacuolation and nuclear pyknosis, intense TUNEL staining of fibroblasts and increased staining of cells and nuclei for 8-OHdG. Lesser or marginal increases in intensity followed staining for HO-1 and IL-1alpha. The IL-1alpha increase was confirmed by ELISA assays of the medium supernatants. Hesperetin and quercetin-3-glucoside reduced changes in H&E, 8-OHdG, TUNEL and IL-1alpha. Quercetin-3-glucoside reduced the amount of IL-1alpha in LSE media. These observations support the use of the selected biomarkers to monitor UV-A1 damage and provide evidence that dietary ingredients could reduce ultraviolet-A radiation-induced damage.
authors.affiliation: Corporate Research, Unilever R&D, Colworth Laboratory, Sharnbrook, Bedfordshire, MK44 1LQ, UK. pim.dekker@unilever.com
authors.forename: Pim
authors.initials: P
authors.lastname: Dekker
authors.forename: William E
authors.initials: WE
authors.lastname: Parish
authors.forename: Martin R
authors.initials: MR
authors.lastname: Green
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis; D006801:Humans; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants