Molecular cloning and sequence analysis of the porcine precursor of endothelin-2.
The amino acid sequences of two of the three endothelin (ET) family peptides, ET-1 and ET-3, are identical among mammals, whereas for the other family member, ET-2 or vasoactive intestinal contractor (VIC), the mouse and rat sequences differ from the human counterpart ET-2 by one amino acid residue. To examine more deeply the structural diversity among ET-2/VIC orthologs (EDN2), we screened porcine ET-2/VIC-like cDNAs using the 5' rapid amplification of cDNA ends (RACE) method with degenerate primers based on ET-2/VIC mature peptides. Sequence analysis of the cDNAs showed that ET-2 is present in pig. The full-length cDNA sequence, produced by combining 5' RACE and 3' RACE products, revealed the porcine precursor protein of ET-2 (PPET-2). Porcine PPET-2, made up of 214 amino acids, includes a 26-residue putative signal sequence, big ET-2, mature ET-2, and ET-2-like peptide. The percent sequence identity of porcine PPET-2 with human PPET-2, and rat or mouse precursor protein of VIC runs between approximately 70% and 74%. ET-2, although expressed in intestine, has no anti-microbial activity.
Saida
Kaname
K
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Japan. k.saida@aist.go.jp
Uchide
Tsuyoshi
T
Zhang
Shaoxuan
S
Adur
Javier
J
Kawano
Yasuhiro
Y
Ogiso
Masayo
M
Oka
Shu-ichi
S
Takizawa
Satoshi
S
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D016232:Endothelins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D007422:Intestines / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013552:Swine
D016232:Endothelins; D011498:Protein Precursors; C099648:preproendothelin 2