title: Effective protection of pigs against lethal Pseudorabies virus infection after a single injection of low-dose Sindbis-derived plasmids encoding PrV gB, gC and gD glycoproteins.
abstract: This study compared protection of pigs against lethal Pseudorabies virus (PrV) infection induced by a single injection of various quantities of Sindbis virus-derived plasmids encoding PrV glycoproteins gB, gC and gD. Pigs were injected with 340, 68 or 13 microg of each plasmid. Few immune differences were observed after DNA injection and more importantly the pigs of the three groups were equally protected against virulent PrV infection. Single-shot injection of 13 microg of each PrV glycoprotein encoding Sindbis virus-derived plasmid is able to effectively protect pigs from PrV infection.
authors.affiliation: Viral Genetics and Biosafety Unit, Afssa, BP-53, F-22440 Ploufragan, France. d.dory@ploufragan.afssa.fr
authors.forename: Daniel
authors.initials: D
authors.lastname: Dory
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Torché
authors.forename: Véronique
authors.initials: V
authors.lastname: Béven
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.forename: Christophe
authors.initials: C
authors.lastname: Loizel
authors.forename: Roland
authors.initials: R
authors.lastname: Cariolet
authors.forename: André
authors.initials: A
authors.lastname: Jestin
mesh_terms: D000818:Animals; D005822:Genetic Vectors / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage* / Q000276:immunology; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D022421:Pseudorabies Vaccines; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins