title: Direct interaction and cooperative role of tumor suppressor p16 with band 3 (AE1).
abstract: By using the C-terminal 112-residue of band 3 to screen the K562 cDNA library, we find that the p16 interacts with band 3, which was confirmed both in yeast and in mammalian cells. Functional experiments show that p16 facilitates the movement of band 3 to plasma membrane with increased anion transport activity in 293t cells. Moreover, expression of endogenous p16 in 293t cells was increased at 24 and 36 h after transfection with band 3. Our findings provide a novel regulation pathway for both band 3 and p16.
authors.affiliation: Department of Pathophysiology, Shanghai Institute of Hematology, Rui-Jin Hospital, Shanghai Second Medical University (SSMU), Shanghai 200025, PR China. guohuifu@shsmu.edu.cn
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Fu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Xi
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Shen
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Pan
authors.forename: Wu-Zhong
authors.initials: WZ
authors.lastname: Shen
authors.forename: Xiao-Shu
authors.initials: XS
authors.lastname: Jiang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D019941:Cyclin-Dependent Kinase Inhibitor p16