title: The relation of the XbaI and PvuII polymorphisms of the estrogen receptor gene and the CAG repeat polymorphism of the androgen receptor gene to peak bone mass and bone turnover rate among young healthy men.
abstract: The genes coding for estrogen receptor-alpha (ER-alpha) and androgen receptors (AR) are potential candidates for the regulation of bone mass and turnover, which may contribute to both the achievement of peak bone mass and bone loss after completion of growth. The present study was aimed at elucidating the role of two restriction fragment lengths (XbaI and PvuII) polymorphisms of the ER gene and the CAG repeat polymorphism of the AR gene as determinants of peak bone mass in men; special attention was paid to the interaction between serum free estradiol (E2) levels and the XbaI and PvuII genotypes. A cross-sectional study, with data on lifestyle factors collected retrospectively, was performed in 234 young men, aged 18.3 to 20.6 years. Of the men, 184 were recruits of the Finnish Army and 50 were men of similar age who had postponed their military service for reasons not related to health. Bone mineral content (BMC), density (BMD) and scan area were measured in the lumbar spine and upper femur by dual-energy X-ray absorptiometry (DXA). The bone turnover rate was assessed by measuring serum type I procollagen aminoterminal propeptide (PINP) and tartrate-resistant acid phosphatase 5b (TRACP5b) as well as urinary excretion of type I collagen aminoterminal telopeptide (NTX). After adjusting for age, height, weight, exercise, smoking, calcium and alcohol intake, BMC, scan area and BMD at all measurement sites were similar for the different XbaI and PvuII genotypes of the ER and independent of the number of the CAG repeats of the AR gene. No association was found between free E2 levels and bone parameters among any genotype group of the XbaI and PvuII polymorphisms. Except for urinary NTX, which showed a tendency to higher values for the xx (P=0.08) and pp (P=0.10) genotypes of the ER, bone turnover markers were not related to the genotypes studied. Our study does not support the view that the XbaI and PvuII polymorphisms of the ER gene and the CAG polymorphism of the AR gene would have a substantial impact on the development of peak bone mass in young Finnish men.
authors.affiliation: Division of Endocrinology, Helsinki University Central Hospital, 00290, Helsinki, Finland.
authors.forename: Ville-Valtteri
authors.initials: VV
authors.lastname: Välimäki
authors.forename: Kirsi
authors.initials: K
authors.lastname: Piippo
authors.forename: Stiina
authors.initials: S
authors.lastname: Välimäki
authors.forename: Eliisa
authors.initials: E
authors.lastname: Löyttyniemi
authors.forename: Kimmo
authors.initials: K
authors.lastname: Kontula
authors.forename: Matti J
authors.initials: MJ
authors.lastname: Välimäki
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015519:Bone Density / Q000502:physiology; D001842:Bone and Bones / Q000502:physiology*; D003094:Collagen / Q000652:urine; D003430:Cross-Sectional Studies; D004958:Estradiol / Q000097:blood; D005269:Femur; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008019:Life Style; D008159:Lumbar Vertebrae; D008297:Male; D008889:Military Personnel; D010446:Peptide Fragments / Q000097:blood; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011347:Procollagen / Q000097:blood; D011944:Receptors, Androgen / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D012189:Retrospective Studies; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D010446:Peptide Fragments; D011347:Procollagen; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; C047202:procollagen Type I N-terminal peptide; C461299:urinary N-telopeptide of type I collagen, human; D004958:Estradiol; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase