title: Identification of the thrombin light chain a as the single best mass for differentiation of gastric cancer patients from individuals with dyspepsia by proteome analysis.
abstract: Gastric cancer mortality is second only to lung cancer, and its prognosis is dismal. Using surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry, we previously identified a single best mass, which could separate gastric cancer from patients without cancer, with a sensitivity of 89.9% and a specificity of 90%. Using protein liquid chromatography systems with various chromatography media and MS/MS analysis, we were able to identify thrombin light chain A, a proteolytic fragment of prothrombin, as the single best mass for early detection of gastric cancer patients. These findings indicate that disturbances in the coagulation-system are early events in gastric cancer biology and that a decrease or loss of thrombin light chain A, which we termed negative serum protein profiling, may contribute to the diagnosis of cancer patients.
authors.affiliation: Department of Gastroenterology, General Surgery and Pathology, Otto-von-Guericke University, D-39120 Magdeburg, Germany. Matthias.Ebert@medizin.uni-magdeburg.de
authors.forename: Matthias P A
authors.initials: MP
authors.lastname: Ebert
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lamer
authors.forename: Jörn
authors.initials: J
authors.lastname: Meuer
authors.forename: Peter
authors.initials: P
authors.lastname: Malfertheiner
authors.forename: Marc
authors.initials: M
authors.lastname: Reymond
authors.forename: Thomas
authors.initials: T
authors.lastname: Buschmann
authors.forename: Christoph
authors.initials: C
authors.lastname: Röcken
authors.forename: Volker
authors.initials: V
authors.lastname: Seibert
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D003937:Diagnosis, Differential; D004415:Dyspepsia / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry
chemical_list: D020543:Proteome; D013917:Thrombin