Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022.
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
Gottfried
Pnina
P
Department of Biochemistry, Tel-Aviv University, Israel.
Lotan
Ofra
O
Kolot
Mikhail
M
Maslenin
Ludmila
L
Bendov
Rosa
R
Gorovits
Rena
R
Yesodi
Vered
V
Yagil
Ezra
E
Rosner
Arie
A
D017360:Arabidopsis / Q000235:genetics*; D025523:Bacteriophage HK022 / Q000201:enzymology*; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D019426:Integrases