title: The KIF3 motor transports N-cadherin and organizes the developing neuroepithelium.
abstract: In the developing brain, the organization of the neuroepithelium is maintained by a critical balance between proliferation and cell-cell adhesion of neural progenitor cells. The molecular mechanisms that underlie this are still largely unknown. Here, through analysis of a conditional knockout mouse for the Kap3 gene, we show that post-Golgi transport of N-cadherin by the KIF3 molecular motor complex is crucial for maintaining this balance. N-cadherin and beta-catenin associate with the KIF3 complex by co-immunoprecipitation, and colocalize with KIF3 in cells. Furthermore, in KAP3-deficient cells, the subcellular localization of N-cadherin was disrupted. Taken together, these results suggest a potential tumour-suppressing activity for this molecular motor.
authors.affiliation: Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Junlin
authors.initials: J
authors.lastname: Teng
authors.forename: Tatemitsu
authors.initials: T
authors.lastname: Rai
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takei
authors.forename: Takao
authors.initials: T
authors.lastname: Nakata
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Hirasawa
authors.forename: Ashok B
authors.initials: AB
authors.lastname: Kulkarni
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hirokawa
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000196:embryology / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D051379:Mice; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology; D046569:Neuroepithelial Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D015534:Trans-Activators / Q000378:metabolism; D051176:beta Catenin
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D020409:Molecular Motor Proteins; D015534:Trans-Activators; D051176:beta Catenin; C100815:Kifap3 protein, mouse