title: Epidemiological study of invasive pulmonary aspergillosis in a haematology unit by molecular typing of environmental and patient isolates of Aspergillus fumigatus.
abstract: In order to determine the possible relationship between environmental contamination by Aspergillus fumigatus and occurrence of invasive aspergillosis, a one-year prospective study was carried out in the haematology ward of Hautepierre Hospital, Strasbourg, France. During the study period, 21 environmental isolates and 26 clinical isolates of A. fumigatus were collected. Each was genotyped using a random amplification of polymorphic DNA (RAPD) technique. Thirty-four distinct profiles were identified by RAPD analysis, indicating the great genetic diversity of A. fumigatus isolated from infected patients and from the environment. For two patients, RAPD analysis demonstrated concurrent infection by at least two different strains. In two cases, a genetic similarity was noted between isolates obtained from a patient and from the environment.
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale, Faculté de Médecine de Strasbourg, Université Louis Pasteur, France. jean.menotti@sls.ap-hop-paris.fr
authors.forename: J
authors.initials: J
authors.lastname: Menotti
authors.forename: J
authors.initials: J
authors.lastname: Waller
authors.forename: O
authors.initials: O
authors.lastname: Meunier
authors.forename: V
authors.initials: V
authors.lastname: Letscher-Bru
authors.forename: R
authors.initials: R
authors.lastname: Herbrecht
authors.forename: E
authors.initials: E
authors.lastname: Candolfi
mesh_terms: D000391:Air Microbiology; D001228:Aspergillosis / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D001232:Aspergillus fumigatus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001706:Biopsy; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D016002:Discriminant Analysis; D004784:Environmental Monitoring / Q000379:methods; D016021:Epidemiologic Studies; D062665:Epidemiological Monitoring; D004866:Equipment Contamination / Q000706:statistics & numerical data*; D005602:France / Q000453:epidemiology; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006405:Hematology; D006748:Hospital Departments; D006801:Humans; D015994:Incidence; D017053:Infection Control / Q000379:methods; D008172:Lung Diseases, Fungal / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D011446:Prospective Studies; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012307:Risk Factors; D013183:Sputum / Q000382:microbiology
chemical_list: D004271:DNA, Fungal