title: Immunocytochemical detection and reverse transcription polymerase chain reaction expression of oncostatin M (OSM) and its receptor (OSM-Rbeta) in human fetal and adult ovaries.
abstract: OBJECTIVE
  To investigate the immunocytochemical expression and presence of mRNA transcripts of oncostatin M (OSM) and its exclusive receptor (OSM-Rbeta) in ovaries from human adults and fetuses.
  
  
  METHODS
  Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study.
  
  
  METHODS
  Major tertiary care and referral academic centers.
  
  
  METHODS
  Ten women and girls undergoing laparoscopic ovarian biopsy and 30 women undergoing second-trimester and third-trimester pregnancy terminations.
  
  
  METHODS
  None.
  
  
  METHODS
  Microscopic morphometric analysis, immunocytochemistry for OSM and OSM-Rbeta, and RT-PCR analyses.
  
  
  RESULTS
  There was strong to moderate immunocytochemical staining for OSM in both oocytes and granulosa cells of follicles from primordial stages onwards in ovaries from both fetuses and adults/adolescents. OSM-Rbeta was detected mainly in the oocytes. Transcripts of OSM and OSM-Rbeta RNA were detected by RT-PCR analyses.
  
  
  CONCLUSIONS
  The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles.
authors.affiliation: Infertility and IVF Unit, Department of Obstetrics and Gynecology, Rabin Medical Center, Beilinson Campus, Petah Tikva 49100, Israel. ronita@clalit.org.il
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
authors.forename: Asangla
authors.initials: A
authors.lastname: Ao
authors.forename: Shaoguang
authors.initials: S
authors.lastname: Jin
authors.forename: Miriam
authors.initials: M
authors.lastname: Barnett
authors.forename: Robert
authors.initials: R
authors.lastname: Van den Hurk
authors.forename: Sarit
authors.initials: S
authors.lastname: Freimann
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D053683:Oncostatin M; D006080:Ovarian Follicle / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism*; D053677:Receptors, Oncostatin M; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C508351:OSM protein, human; D010455:Peptides; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053677:Receptors, Oncostatin M; D053683:Oncostatin M