title: No evidence of cross-reactivity of human antibodies to a 33-mer peptide of the alpha-gliadin component of gluten with Bordetella pertussis pertactin.
abstract: A 33-mer peptide of the alpha-gliadin component of gluten was recently identified as primary initiator of the inflammatory response to gluten in coeliac disease (CD) patients. This proline-glutamine-rich peptide (PG-peptide) is highly homologous to internal sequence of pertactin, an immunogenic protein of Bordetella pertussis. Using enzyme immunoassays, we measured serum antibodies to pertactin and to PG-peptide in 167 Finnish subjects including pertussis vaccine recipients and pertussis patients, CD and non-CD patients and healthy individuals. We found no cross-reactivity between human antibodies to the two different components, suggesting that neither pertussis immunization nor disease contributes to the pathogenesis of CD.
authors.affiliation: Department of Human Microbial Ecology and Inflammation, National Public Health Institute, Turku, Finland. qiushui.he@ktl.fi
authors.forename: Qiushui
authors.initials: Q
authors.lastname: He
authors.forename: Matti K
authors.initials: MK
authors.lastname: Viljanen
authors.forename: Ari E
authors.initials: AE
authors.lastname: Hinkkanen
authors.forename: Heikki
authors.initials: H
authors.lastname: Arvilommi
authors.forename: Jussi
authors.initials: J
authors.lastname: Mertsola
authors.forename: Markku
authors.initials: M
authors.lastname: Viander
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001886:Bordetella pertussis / Q000737:chemistry / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005387:Finland; D005903:Gliadin / Q000276:immunology*; D005983:Glutens / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010566:Virulence Factors, Bordetella; C068929:pertactin; D005983:Glutens; D005903:Gliadin