High-throughput screens and selections of enzyme-encoding genes.
The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.
Aharoni
Amir
A
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Griffiths
Andrew D
AD
Tawfik
Dan S
DS
D000818:Animals; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D006801:Humans
D004798:Enzymes