3'-immobilized probes with 2'-caps: synthesis of oligonucleotides with 2'-N-methyl-2'-(anthraquinone carboxamido)uridine residues.
[reaction: see text] A synthesis for oligodeoxynucleotides with a 3'-terminal 2'-N-methyl-2'-acylamido-2'-deoxyuridine residue was developed. Unlike their unmethylated counterparts, these oligodeoxynucleotides can be stably immobilized on aldehyde-displaying glass surfaces to provide DNA microarrays. An anthraquinone carboxamido group as a 2'-substituent doubled the capture efficiency of an immobilized tetradecamer.
Al-Rawi
Samy
S
Institute for Organic Chemistry, Universität Karlsruhe (TH), D-76131 Karlsruhe, Germany.
Ahlborn
Carolin
C
Richert
Clemens
C
D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D015342:DNA Probes / Q000138:chemical synthesis*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D020411:Oligonucleotide Array Sequence Analysis; D014529:Uridine / Q000138:chemical synthesis*
D000880:Anthraquinones; D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D014529:Uridine