Complement inhibiting properties of dragon's blood from Croton draco.
The latex of Croton draco, its extracts and several latex components have been investigated for their influence on both classical (CP) and alternative (AP) activation pathways of the complement system using a hemolytic assay. The best inhibition was found for the classical pathway. The latex, ethyl acetate and ethyl ether extracts exhibited extremely high inhibition on the CP (94, 90 and 77%, respectively) at a concentration of 1 mg/ml. The flavonoid myricitrin, the alkaloid taspine and the cyclopeptides P1 and P2 showed high inhibition on CP (83, 91, 78 and 63%, respectively) at a concentration of 0.9 mM.
Tsacheva
Ivanka
I
Department of Biochemistry, Faculty of Biology, Sofia University, 1164 Sofia, Bulgaria.
Rostan
Joerg
J
Iossifova
Tania
T
Vogler
Bernhard
B
Odjakova
Mariela
M
Navas
Hernan
H
Kostova
Ivanka
I
Kojouharova
Michaela
M
Kraus
Wolfgang
W
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology; D003167:Complement Activation / Q000187:drug effects*; D003169:Complement Inactivator Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D029802:Croton / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine
D000470:Alkaloids; D003169:Complement Inactivator Proteins; D005419:Flavonoids; D010456:Peptides, Cyclic; D002392:Catechin; C018394:taspine