title: Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry.
abstract: The availability of genome sequence offers the opportunity to further expand our knowledge about proteins expressed by Rickettsia conorii, strictly intracellular bacterium responsible for Mediterranean spotted fever. Using two-dimensional polyacrylamide gel electrophoresis combined with MALDI-TOF mass spectrometry, we established the first reference map of R. conorii proteome. This approach also allowed identification of GroEL as the major antigen recognized by rabbit serum and sera of infected patients. Altogether, this work opens the way to characterize the proteome of R. conorii, to compare protein profiles of different isolates or of bacteria maintained under different experimental conditions and to identify immunogenic proteins as potential vaccine targets.
authors.affiliation: Unité des Rickettsies, CNRS UMR 6020, IFR-48, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille, France. patricia.renesto@medecine.univ-mrs.fr
authors.forename: Patricia
authors.initials: P
authors.lastname: Renesto
authors.forename: Saïd
authors.initials: S
authors.lastname: Azza
authors.forename: Alain
authors.initials: A
authors.lastname: Dolla
authors.forename: Patrick
authors.initials: P
authors.lastname: Fourquet
authors.forename: Guy
authors.initials: G
authors.lastname: Vestris
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Gorvel
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis*; D018834:Chaperonin 60 / Q000032:analysis / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020543:Proteome / Q000032:analysis*; D011817:Rabbits; D020114:Rickettsia conorii / Q000737:chemistry* / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D020543:Proteome