South West Indian Ocean islands tomato begomovirus populations represent a new major monopartite begomovirus group.
Biological and molecular properties of Tomato leaf curl Madagascar virus isolates from Morondova and Toliary (ToLCMGV-[Tol], -[Mor]), Tomato leaf curl Mayotte virus isolates from Dembeni and Kahani (ToLCYTV-[Dem], -[Kah]) and a Tomato yellow leaf curl virus isolate from Reunion (TYLCV-Mld[RE]) were determined. Full-length DNA components of the five isolates from Madagascar, Mayotte and Reunion were cloned and sequenced and, with the exception of ToLCMGV-[Tol], were shown to be both infectious in tomato and transmissible by Bemisia tabaci. Sequence analysis revealed that these viruses had genome organizations of monopartite begomoviruses and that both ToLCMGV and ToLCYTV belong to the African begomoviruses but represent a distinct monophyletic group that we have tentatively named the South West islands of the Indian Ocean (SWIO). All of the SWIO isolates examined were apparently complex recombinants. None of the sequences within the recombinant regions closely resembled that of any known non-SWIO begomovirus, suggesting an isolation of these virus populations.
Delatte
Hélène
H
CIRAD, UMR C53 PVBMT, CIRAD-Université de la Réunion, Pôle de Protection des Plantes, Ligne Paradis, 97410 Saint Pierre, Réunion, France.
Martin
Darren P
DP
Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory 7925, South Africa.
Naze
Florence
F
CIRAD, UMR C53 PVBMT, CIRAD-Université de la Réunion, Pôle de Protection des Plantes, Ligne Paradis, 97410 Saint Pierre, Réunion, France.
Goldbach
Rob
R
Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
Reynaud
Bernard
B
CIRAD, UMR C53 PVBMT, CIRAD-Université de la Réunion, Pôle de Protection des Plantes, Ligne Paradis, 97410 Saint Pierre, Réunion, France.
Peterschmitt
Michel
M
CIRAD, UMR BGPI, CIRAD-INRA, TA 41/K, 34398 Montpellier Cedex 5, France.
Lett
Jean-Michel
JM
CIRAD, UMR C53 PVBMT, CIRAD-Université de la Réunion, Pôle de Protection des Plantes, Ligne Paradis, 97410 Saint Pierre, Réunion, France.
D000818:Animals; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D018562:Disease Transmission, Infectious; D004199:Disease Vectors; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006430:Hemiptera / Q000821:virology; D007195:Indian Ocean Islands; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004279:DNA, Viral