Expression of a self-processing, pathogen resistance-enhancing gene construct in Arabidopsis.
A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclusion-a (NIa) proteinase of tobacco etch virus (TEV). The protein coding sequences used were: a Trichoderma harzinum endochitinase, a truncated NIa proteinase of TEV, and a wheat oxalate oxidase. When p35S-CNO construct was tested in Arabidopsis thaliana, the polyprotein was properly cleaved after translation and the products exhibited functional enzymatic activity in vivo.
Liang
Haiying
H
School of Forest Resources, The Pennsylvania State University, University Park, PA 16802, USA.
Gao
Hongyu
H
Maynard
Charles A
CA
Powell
William A
WA
D017360:Arabidopsis / Q000235:genetics*; D015153:Blotting, Western; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D036226:Seedlings / Q000201:enzymology; D013347:Subcellular Fractions; D014908:Triticum / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D011994:Recombinant Proteins; D014764:Viral Proteins; D010088:Oxidoreductases; C020598:oxalate oxidase; D002688:Chitinases; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses