Identification of the thrombin light chain a as the single best mass for differentiation of gastric cancer patients from individuals with dyspepsia by proteome analysis.
Gastric cancer mortality is second only to lung cancer, and its prognosis is dismal. Using surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry, we previously identified a single best mass, which could separate gastric cancer from patients without cancer, with a sensitivity of 89.9% and a specificity of 90%. Using protein liquid chromatography systems with various chromatography media and MS/MS analysis, we were able to identify thrombin light chain A, a proteolytic fragment of prothrombin, as the single best mass for early detection of gastric cancer patients. These findings indicate that disturbances in the coagulation-system are early events in gastric cancer biology and that a decrease or loss of thrombin light chain A, which we termed negative serum protein profiling, may contribute to the diagnosis of cancer patients.
Ebert
Matthias P A
MP
Department of Gastroenterology, General Surgery and Pathology, Otto-von-Guericke University, D-39120 Magdeburg, Germany. Matthias.Ebert@medizin.uni-magdeburg.de
Lamer
Stephanie
S
Meuer
Jörn
J
Malfertheiner
Peter
P
Reymond
Marc
M
Buschmann
Thomas
T
Röcken
Christoph
C
Seibert
Volker
V
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D003937:Diagnosis, Differential; D004415:Dyspepsia / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry
D020543:Proteome; D013917:Thrombin