Effect of somatostatin on duodenal glucose absorption in man.
OBJECTIVE
The hyperglycemic hyperinsulinemic clamp technique using intraduodenally infused glucose is an attractive tool for studying postprandial glucose metabolism under strictly controlled conditions. Because it requires the use of somatostatin (SST), we examined, in this study, the effect of SST on intestinal glucose absorption.


BACKGROUND
Twenty-six normal volunteers were given a constant 3-h intraduodenal infusion of glucose (6 mg.kg(-1).min(-1)) labeled with [2-(3)H]glucose for glucose absorption measurement. During glucose infusion, 19 subjects received iv SST at doses of 10-100 ng.kg(-1).min(-1) plus insulin and glucagon, and seven subjects were studied under control conditions. In the controls, glucose was absorbed at a rate that, after a 20-min lag period, equaled the infusion rate.


RESULTS
With all the doses of SST tested, absorption was considerably delayed but equaled the rate of infusion after 3 h. At that time, only 5 +/- 2% of the total amount of infused glucose was unabsorbed in the control subjects vs. 36 +/- 2% (P < 0.001) in the SST-infused subjects. In the latter, the intraluminal residue was almost totally absorbed within 40 min of the cessation of SST infusion. At the lowest dose of SST tested (10 ng.kg(-1).min(-1)), suppression of insulin secretion was incomplete.


CONCLUSIONS
These properties of SST hamper the use of intraduodenal hyperglycemic hyperinsulinemic clamps as a tool for exploring postprandial glucose metabolism.
Féry
Francoise
F
Laboratory of Experimental Medicine and Department of Endocrinology, Hôpital Erasme, University of Brussels, Brussels, Belgium. ffery@ulb.ac.be
Tappy
Luc
L
Schneiter
Philippe
P
Devière
Jacques
J
Balasse
Edmond O
EO
D000328:Adult; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D013004:Somatostatin / Q000494:pharmacology*
D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D013004:Somatostatin; D005947:Glucose