title: [Immunoprotection induced by recombinant plasmids pVAX1-Mzp5-7 of Eimeria tenella].
abstract: OBJECTIVE
  To observe the immunoprotection of recombinant plasmids of lambda Mzp5-7 to chicken challenged with Eimeria tenella (E. t) oocysts.
  
  
  METHODS
  All the chickens were immunized with the recombinant plasmids by different inoculation pathways, three times on d7, d14 and d21, and challenged with E. t oocysts. The response of specific humoral and cell immunity were detected by the immunological methods such as the specific antibody response, the ratio of CD4+/CD8+ T cells.
  
  
  RESULTS
  The results showed that the recombinant plasmids can induce immune response. The specific immune responses were strengthened with the increase of immunization times. The difference of the immunity indexes among experiment groups was not significant, while the ratio of CD4+/CD8+ T cells and antibody titer were significantly higher in experiment groups than those in the control groups. The number of oocysts shed in experiment groups decreased and the shedding duration was shorter significantly. The body weight of the chicken in experiment groups increased significantly and the lesion in cecum was slighter than that of control groups.
  
  
  CONCLUSIONS
  The recombinant plasmids of lambda Mzp5-7 show an immunoprotection to chicken challenged with E. t oocysts.
authors.affiliation: Faculty of Military Veterinary Medicine, Quartermaster University of PLA, Changchun 130062, China.
authors.forename: Rui-ling
authors.initials: RL
authors.lastname: Qin
authors.forename: Xi-chen
authors.initials: XC
authors.lastname: Zhang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Li
authors.forename: Zhan-yun
authors.initials: ZY
authors.lastname: Xu
authors.forename: Ji-gang
authors.initials: JG
authors.lastname: Yin
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D016516:CD4-CD8 Ratio; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control*; D016786:Eimeria tenella / Q000276:immunology*; D008297:Male; D010957:Plasmids / Q000276:immunology*; D011995:Recombination, Genetic; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D019444:Vaccines, DNA; C061194:Eimeria tenella sporozoite antigen