Stearoyl-CoA desaturase 1 deficiency increases CTP:choline cytidylyltransferase translocation into the membrane and enhances phosphatidylcholine synthesis in liver.
Stearoyl-CoA desaturase (SCD) is the rate-limiting enzyme in monounsaturated fatty acid synthesis. Previously, we showed that Scd1 deficiency reduces liver triglyceride accumulation and considerably decreases synthesis of very low density lipoprotein and its secretion in both lean and obese mice. In the present study, we found that Scd1 deficiency significantly modulates hepatic glycerophospholipid profile. The content of phosphatidylcholine (PC) was increased by 40% and the activities of CTP:choline cytidylyltransferase (CCT), the rate-limiting enzyme in de novo PC synthesis, and choline phosphotransferase were increased by 64 and 53%, respectively, in liver of Scd1-/- mice. In contrast, the protein level of phosphatidylethanolamine N-methyltransferase, an enzyme involved in PC synthesis via methylation of phosphatidylethanolamine, was decreased by 80% in the liver of Scd1-/- mice. Membrane translocation of CCT is required for its activation. Immunoblot analyses demonstrated that twice as much CCTalpha was associated with plasma membrane in livers of Scd1-/- compared with wild type mice, suggesting that Scd1 mutation leads to an increase in CCT membrane affinity. The incorporation of [(3)H]glycerol into PC was increased by 2.5-fold in Scd1-/- primary hepatocytes compared with those of wild type mice. Furthermore, mitochondrial glycerol-3-phosphate acyltransferase activity was reduced by 42% in liver of Scd1-/- mice; however, the activities of microsomal glycerol-3-phosphate acyltransferase, diacylglycerol acyltransferase, and ethanolamine phosphotransferase were not affected by Scd1 mutation. Our study revealed that SCD1 deficiency specifically increases CCT activity by promoting its translocation into membrane and enhances PC biosynthesis in liver.
Dobrzyn
Agnieszka
A
Department of Biochemistry, University of Wisconsin-Madison, 53706, USA.
Dobrzyn
Pawel
P
Miyazaki
Makoto
M
Sampath
Harini
H
Chu
Kiki
K
Ntambi
James M
JM
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism*; D051048:Diacylglycerol O-Acyltransferase; D004982:Ethanolaminephosphotransferase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D006720:Homozygote; D015151:Immunoblotting; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009154:Mutation; D010713:Phosphatidylcholines / Q000096:biosynthesis* / Q000378:metabolism; D021381:Protein Transport; D013230:Stearoyl-CoA Desaturase / Q000172:deficiency*; D019076:Transgenes
D005227:Fatty Acids; D010713:Phosphatidylcholines; D013230:Stearoyl-CoA Desaturase; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase; D019916:Choline-Phosphate Cytidylyltransferase; D004982:Ethanolaminephosphotransferase; D005990:Glycerol