title: Dynein light chain 1 regulates dynamin-mediated F-actin assembly during sperm individualization in Drosophila.
abstract: Toward the end of spermiogenesis, spermatid nuclei are compacted and the clonally related spermatids individualize to become mature and active sperm. Studies in Drosophila showed that caudal end-directed movement of a microfilament-rich structure, called investment cone, expels the cytoplasmic contents of individual spermatids. F-actin dynamics plays an important role in this process. Here we report that the dynein light chain 1 (DLC1) of Drosophila is involved in two separate cellular processes during sperm individualization. It is enriched around spermatid nuclei during postelongation stages and plays an important role in the dynein-dynactin-dependent rostral retention of the nuclei during this period. In addition, DDLC1 colocalizes with dynamin along investment cones and regulates F-actin assembly at this organelle by retaining dynamin along the cones. Interestingly, we found that this process does not require the other subunits of cytoplasmic dynein-dynactin complex. Altogether, these observations suggest that DLC1 could independently regulate multiple cellular functions and established a novel role of this protein in F-actin assembly in Drosophila.
authors.affiliation: Tata Institute of Fundamental Research, Mumbai 400 005, India.
authors.forename: Anindya
authors.initials: A
authors.lastname: Ghosh-Roy
authors.forename: Bela S
authors.initials: BS
authors.lastname: Desai
authors.forename: Krishanu
authors.initials: K
authors.lastname: Ray
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000483:Alleles; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000072159:Dynactin Complex; D034281:Dynamins / Q000378:metabolism*; D004398:Dyneins / Q000737:chemistry; D008297:Male; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000737:chemistry; D011485:Protein Binding; D013087:Spermatids / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D000199:Actins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; C542608:ctp protein, Drosophila; D004398:Dyneins; D034281:Dynamins