Antigen recognition determinants of gammadelta T cell receptors.
The molecular basis of gammadelta T cell receptor (TCR) recognition is poorly understood. Here, we analyze the TCR sequences of a natural gammadelta T cell population specific for the major histocompatibility complex class Ib molecule T22. We find that T22 recognition correlates strongly with a somatically recombined TCRdelta complementarity-determining region 3 (CDR3) motif derived from germ line-encoded residues. Sequence diversity around these residues modulates TCR ligand-binding affinities, whereas V gene usage correlates mainly with tissue origin. These results show how an antigen-specific gammadelta TCR repertoire can be generated at a high frequency and suggest that gammadelta T cells recognize a limited number of antigens.
Shin
Sunny
S
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
El-Diwany
Ramy
R
Schaffert
Steven
S
Adams
Erin J
EJ
Garcia
K Christopher
KC
Pereira
Pablo
P
Chien
Yueh-Hsiu
YH
D000595:Amino Acid Sequence; D000941:Antigens; D001665:Binding Sites; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D011487:Protein Conformation; D011506:Proteins / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D015395:Histocompatibility Antigens Class I; D008024:Ligands; D011506:Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; C089395:T22 antigen