title: Evidence for icaADBC-independent biofilm development mechanism in methicillin-resistant Staphylococcus aureus clinical isolates.
abstract: Synthesis of a polysaccharide adhesin by icaADBC-encoded enzymes is currently the best-understood mechanism of staphylococcal biofilm development. In four methicillin-resistant Staphylococcus aureus isolates, environmental activation of icaADBC did not always correlate with increased biofilm production. Moreover, glucose-mediated biofilm development in these isolates was icaADBC independent. Apparently, an environmentally regulated, ica-independent mechanism(s) of biofilm development exists in S. aureus clinical isolates.
authors.affiliation: Department of Clinical Microbiology, Education and Research Centre, Smurfit Building, Beaumont Hospital, Royal College of Surgeons in Ireland, Dublin 9, Ireland.
authors.forename: Fidelma
authors.initials: F
authors.lastname: Fitzpatrick
authors.forename: Hilary
authors.initials: H
authors.lastname: Humphreys
authors.forename: James P
authors.initials: JP
authors.lastname: O'Gara
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006801:Humans; D016106:Methicillin Resistance; D009876:Operon; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin; D005947:Glucose