Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.
O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion.
Perlińska-Lenart
Urszula
U
Laboratory of Fungal Glycobiology, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland.
Kurzatkowski
Wiesław
W
Janas
Piotr
P
Kopińska
Agnieszka
A
Palamarczyk
Grazyna
G
Kruszewska
Joanna S
JS
D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001483:Base Sequence; D017931:DNA Primers; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006031:Glycosylation; D046529:Microscopy, Electron, Transmission; D009154:Mutation
D017931:DNA Primers; D005656:Fungal Proteins