IL-1beta stimulates argininosuccinate synthetase gene expression through NF-kappaB in Caco-2 cells.
Argininosuccinate synthetase (ASS) is limiting the arginine synthesis and can be stimulated by immunostimulants. We previously identified a putative NF-kappaB element in the human ASS gene promoter but its functionality was unknown (Husson et al., Eur. J. Biochem. 270 (2003) 1887). In the present study, using Caco-2 cells, a human enterocyte line, we demonstrate that IL-1beta rapidly induces the expression of the ASS gene at a transcriptional level through NF-kappaB activation. Using gel shift assay and double-strand oligonucleotide sequence of the identified putative NF-kappaB binding site of the ASS promoter, we provide evidence that NF-kappaB may functionally interact with this element.
Brasse-Lagnel
Carole
C
Groupe Appareil Digestif, Environnement et Nutrition, EA 3234, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (23), Université de Rouen, Rouen, France.
Lavoinne
Alain
A
Fairand
Alain
A
Vavasseur
Karine
K
Husson
Annie
A
D001124:Argininosuccinate Synthase / Q000235:genetics*; D018938:Caco-2 Cells / Q000187:drug effects / Q000201:enzymology; D024202:Electrophoretic Mobility Shift Assay; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
D007375:Interleukin-1; D016328:NF-kappa B; D001124:Argininosuccinate Synthase