title: Complete genome sequences of two new variants of Grapevine rupestris stem pitting-associated virus and comparative analyses.
abstract: Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have not been determined. Recently, many of the indicator "St George" plants (Vitis rupestris) used throughout the world to index RSP tested positive for GRSPaV. This finding questions the validity of past biological indexing results. In this work, a representative genomic region of GRSPaV was first sequenced from ten "St George" plants from two sources and it was demonstrated that nine of them carried a new variant, GRSPaV-SG1. The genomes of GRSPaV-SG1 and GRSPaV-BS from "Bertille Seyve 5563" plants were sequenced, revealing a genome structure identical to that of GRSPaV-1. It was demonstrated experimentally that infection of "St George" plants with GRSPaV-SG1 is asymptomatic and thus it is proposed that GRSPaV-SG1 infection should not have interfered with the outcome of past indicator indexing. This represents the first attempt to link a GRSPaV variant with pathological properties.
authors.affiliation: Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Ontario, Canada N1G 2W1.|Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456-0462, USA.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Meng
authors.affiliation: Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456-0462, USA.
authors.forename: Caihong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Ontario, Canada N1G 2W1.
authors.forename: Weizhou
authors.initials: W
authors.lastname: Wang
authors.affiliation: Agricultural Research Council, Plant Protection Research Institute, Private Bag X 134, Pretoria 0001, South Africa.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Goszczynski
authors.affiliation: Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456-0462, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Gonsalves
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D027843:Vitis / Q000821:virology*
chemical_list: D018076:DNA, Complementary