title: Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis.
abstract: An electrochemiluminescence (ECL) inhibition method was developed as an indirect detection method for the determination of dopamine and epinephrine separated by capillary electrophoresis (CE). When the concentration of Ru(bpy)(3) (2+) was 50 muM diluted by 50 mM phosphate (pH 8.5) in the cell and 0.5 M tripropylamine (TPA) was added to the running buffer (10 mM phosphate, pH 9.0), an inhibition of ECL of the Ru(bpy)(3) (2+)/TPA system by the analytes was observed. Under the optimized conditions, the relative standard deviations of migration time and negative peak area were less than 1% and 3%, respectively, for 1 microM dopamine or 1 microM epinephrine (n = 10). Linear ranges of 0.1-10 microM for both analytes and the detection limits (signal-to-noise ratio S/N = 3) of 10 nM for dopamine and 30 nM for epinephrine were obtained.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. ekwang@ciac.jl.cn
authors.forename: Jianzhen
authors.initials: J
authors.lastname: Kang
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D004298:Dopamine / Q000032:analysis*; D019075:Electrophoresis, Capillary; D004837:Epinephrine / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D011437:Propylamines / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D011437:Propylamines; C415768:tris(2,2'-bipyridyl)ruthenium(II); C096226:tripropylamine; D004298:Dopamine; D004837:Epinephrine