title: Neurovascular alignment in adult mouse skeletal muscles.
abstract: OBJECTIVE
  Muscle blood flow increases with motor unit recruitment. The physical relationships between somatic motor nerves, which control muscle fiber contraction, and arterioles, which control microvascular perfusion, are unexplored. The authors tested the hypothesis that motor axons align with arterioles in adult skeletal muscle.
  
  
  METHODS
  Transgenic mice (C57BL/6 background, n = 5; 10 months of age) expressing yellow fluorescent protein in all motor nerves underwent vascular casting (Microfil). Excised epitrochlearis, gracilis, gluteus maximus, and spinotrapezius muscles were imaged at 380x and 760x and a computer-integrated tracing system (Neurolucida) was used to acquire 3-dimensional digital renderings of entire arteriolar and neural networks within each muscle.
  
  
  RESULTS
  Arteriolar networks were typically approximately 3-fold longer than neural networks. Nerves coursed with arterioles until terminating at motor endplates. Across muscles, proximity analyses revealed that approximately 75% of total nerve length (9.8-48.8 mm) lay within 200 microm of the nearest arteriole (diameters of 15-60 microm).
  
  
  CONCLUSIONS
  Somatic motor nerves and arterioles align closely within adult mammalian skeletal muscle. Understanding the signals governing neurovascular alignment may hold important clues for the advancement of tissue engineering and regeneration.
authors.affiliation: The John B. Pierce Laboratory & Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06519, USA.
authors.forename: Shawn E
authors.initials: SE
authors.lastname: Bearden
authors.forename: Steven S
authors.initials: SS
authors.lastname: Segal
mesh_terms: D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology*; D001369:Axons; D019521:Body Patterning / Q000502:physiology*; D021621:Imaging, Three-Dimensional; D051379:Mice; D008822:Mice, Transgenic; D009045:Motor Endplate; D009046:Motor Neurons; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000294:innervation*; D010525:Peripheral Nerves / Q000033:anatomy & histology*