title: Sarcocystis neurona-associated meningoencephalitis and description of intramuscular sarcocysts in a fisher (Martes pennanti).
abstract: A free-ranging juvenile fisher (Martes pennanti) with ataxia, lethargy, stupor, and intermittent, whole-body tremors was examined postmortem. Microscopically, the fisher had protozoal meningoencephalitis caused by Sarcocystis neurona, which was confirmed by immunohistochemistry, polymerase chain reaction (PCR) and restriction fragment length polymorphism testing, and genetic sequencing. Sarcocysts found in the skeletal muscle of the fisher were negative for S. neurona by PCR, but were morphologically similar to previous light and electron microscopy descriptions of S. neurona. This is the first report of clinical neural S. neurona infection in a fisher.
authors.affiliation: Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, The University of Georgia, Athens, Georgia 30602, USA. rgerhold@vet.uga.edu
authors.forename: Richard W
authors.initials: RW
authors.lastname: Gerhold
authors.forename: Elizabeth W
authors.initials: EW
authors.lastname: Howerth
authors.forename: David S
authors.initials: DS
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D020808:Central Nervous System Protozoal Infections / Q000175:diagnosis / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry; D017809:Fatal Outcome; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D045969:Mustelidae / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012522:Sarcocystis / Q000235:genetics / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000175:diagnosis / Q000401:mortality / Q000473:pathology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan