Real-time reporting of circadian-regulated gene expression by luciferase imaging in plants and mammalian cells.
Luciferase enzymes have been used as reporters of circadian rhythms in organisms as diverse as cyanobacteria, plants, fruit flies, and mice. This article details methodology for real-time reporting of circadian-regulated gene expression by imaging of luciferase bioluminescence in plants and mammalian cells.
Welsh
David K
DK
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Imaizumi
Takato
T
Kay
Steve A
SA
D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D002940:Circadian Rhythm / Q000502:physiology*; D005583:Fourier Analysis; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D051379:Mice; D008822:Mice, Transgenic; D030821:Plants, Genetically Modified / Q000201:enzymology
D008156:Luciferases