Osteoblastic proliferative activity of Epimedium brevicornum Maxim.
The effect of the extracts of Epimedium brevicornum Maxim. was investigated on proliferative activity in vitro. The osteoblast-like UMR106 cells was employed as an osteoblast model. The EtOH extract and the n-butanol fraction from the crude extract were found to show proliferation stimulating activity. Three flavonoid compounds (icariin, epimedin B and epimedin C) were isolated from this fraction by activity-guided assay, and the effects on cell proliferation were studied. Icariin produced the most significant promoting effect on the proliferation in osteoblast-like UMR106 cells. The results suggested that E. brevicornum Maxim. extracts might have potential activity against osteoporosis, and flavonoids such as icariin might be the active constituents stimulating osteoblasts.
Meng
F H
FH
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Li
Y B
YB
Xiong
Z L
ZL
Jiang
Z M
ZM
Li
F M
FM
D016723:Bone Remodeling; D049109:Cell Proliferation / Q000187:drug effects*; D034181:Epimedium; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy / Q000473:pathology; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D010936:Plant Extracts