[Extraction and analysis of sargassum hemiphyllum polysaccharides].
Sargassum hemiphyllum polysaccharides (SHP) was extracted by thermal water method and the physical and chemical characters, the extraction rate, contents, compositions of SHP was studied. The result showed that SHP was ashen powder, water-soluble, insoluble in organic solvents. The reaction of iodide-potassium iodide was negative to evaluate that SHP was nonstarch polysaccharides. Extraction rate was 7.04% and the content of polysaccharides was 82.9%. Ultraviolet spectrum showed that there were little DNA and protein. Infrared spectroscopy showed that SHP was main pyrano polysaccharides and had beta-linked glycopolysaccharides in molecule structure of SHP. Thin layer chromatography traced that SHP could be xylan. The results indicated that the extract was not only polysaccharides but also higher purity and the method was high efficiency.
Meng
Qing-yong
QY
Analysis Center of Guangdong Medical College, Zhanjiang 524023, China.
Liu
Zhi-hui
ZH
Xu
Mei-yi
MY
Dongye
Guang-zhi
GZ
D002245:Carbon Dioxide / Q000032:analysis; D019411:Clinical Laboratory Techniques; D010936:Plant Extracts; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D044647:Sargassum / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013678:Technology, Pharmaceutical; D014990:Xylans / Q000737:chemistry*
D010936:Plant Extracts; D011134:Polysaccharides; D012997:Solvents; D014990:Xylans; D002245:Carbon Dioxide