Physical and partial genetic map of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) genome.
A Nicaraguan isolate of Spodoptera frugiperda multicapsid nucleopolyhedrovirus (SfMNPV) is undergoing field trials for control of this pest in the Americas. This isolate is composed of multiple genotypes, some of which are deletion mutants. Identification of the genetic changes in deleted genotypes cannot be accomplished without the construction of a detailed physical map. In the present study, combinations of restriction endonuclease analysis and Southern blot analysis was performed. This map was refined by sequencing the termini of cloned restriction fragments. The SfMNPV genome was estimated to be 129.3 kb, 8 kb larger than the previously characterized Sf-2 variant from the United States, due to a deletion between 14.8 and 21.0 m.u. in the physical map described in this study. A total of 27.92 kb were sequenced, which represented 21.5% of the whole genome and included 38 ORFs. Comparison with other sequenced baculoviruses revealed that SfMNPV displayed the highest sequence identity (66%) and gene arrangement (78%) with Spodoptera exigua MNPV, sharing 36 putative ORFs. In addition, the genome organization was similar to that of SeMNPV, with minor differences. Phylogenetic analysis confirmed the close relatedness between SeMNPV and SfMNPV, suggesting they evolved from a common ancestor.
Simón
Oihane
O
Depto. de Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain.
Chevenet
François
F
Williams
Trevor
T
Caballero
Primitivo
P
López-Ferber
Miguel
M
D000818:Animals; D015139:Blotting, Southern; D023061:Gene Order; D016679:Genome, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D018411:Spodoptera / Q000821:virology*