[Molecular cloning of a protein antigen gene of Cysticercus cellulosae].
OBJECTIVE
To immunoscreen one protein antigen gene from a cDNA library of Cysticercus cellulosae.


METHODS
A eDNA library of C. cellulosae was constructed after cDNA was synthesized, and immunoscreened using rabbit anti-C. cellulosae polyclone antibody. The gene structure and its possible function were analyzed by comparing with the sequences available in the GenBank, after the insert of positive clone was subcloned to pBluescript SK plasmid and the nucleotide sequence of the cDNA was determined by dideoxynucleotide chain termination method using a Taq DyeDeoxy Terminator Cycle Sequencing Kit. The amino acid sequence was deduced from nucleotide sequence using GENETYX software. Homological search of the nucleotide sequences was done using BLAST in GenBank.


CONCLUSIONS
A cDNA clone of 1320 bp was isolated. The clone contained one open reading frame composed of 1260 bp encoding 420 amino acids, in which two potential glycosylation sites were found. The partial nucleotide sequence of the gene fragment showed high homology with the essential spectrin gene of Caenorhabditis elegans and the erythrocyte surface antigen gene of Plasmodium falciparum, when the gene fragment was homologically analyzed in GenBank.
Liu
Dian-wu
DW
Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017, China.
Li
Wen-ling
WL
Zhang
Li-mei
LM
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003552:Cysticercus / Q000235:genetics / Q000276:immunology*; D015723:Gene Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D000947:Antigens, Helminth