Sodium pertechnetate (Na99mTcO4) biodistribution in mice exposed to cigarette smoke.
BACKGROUND: The biological effects of cigarette smoke are not fully known. To improve our understanding of the action of various chemical agents, we investigated the biodistribution of sodium pertechnetate (Na99mTcO4) in mice exposed to cigarette smoke. METHODS: Fifteen BALB/c male mice were exposed to the smoke of nine whole commercial cigarettes per day, 3 times/day, for up to 10 days to whole body exposure in a chamber. A control group of 5 BALB/c male mice was sham-smoked. One day later, the exposed and control groups of mice received (7.4 MBq/0.3 ml) of Na99mTcO4 before being killed at 30 min. Bones, brain, heart, intestine, kidney, liver, lungs, muscle, pancreas, spleen, stomach, testis and thyroid were weighed and these organs and blood radioactivity recorded with a gamma counter. The percentage per gram of tissue of injected dose (%ID/g) was determined for each organ. RESULTS: Cigarette smoke significantly decreased (p < 0.05) the %ID/g in red blood cells, bone, kidney, lung, spleen, stomach, testis and thyroid of the exposed mice. CONCLUSION: The toxic effects of cigarette smoke reduced the Na99mTcO4 biodistribution.
Valenca
Samuel S
SS
Departamento de Histologia e Embriologia, Universidade do Estado do Rio de Janeiro, Av, Prof, Manoel de Abreu 444 3° andar – Rio de Janeiro, RJ – 20551-170 Brasil. valenc@zipmail.com.br.
Lima
Elaine Ac
EA
Dire
Gláucio F
GF
Bernardo-Filho
Mário
M
Porto
Luís Cristóvão
LC