Identification of a conserved Moraxella catarrhalis haemoglobin-utilization protein, MhuA.
Moraxella catarrhalis is a leading cause of acute otitis media in children and is a cause of respiratory disease in adults with underlying lung disease. This organism is a strict human pathogen that has an absolute requirement for iron in order to grow and cause disease. Previous studies identified transferrin and lactoferrin receptors used by M. catarrhalis to obtain iron from the human host, yet other iron-acquisition systems remain undefined. In this study, it is demonstrated that this strict mucosal pathogen can utilize haemoglobin (Hb) as a sole source of iron for growth. A novel 107 kDa outer-membrane protein involved in Hb utilization by this pathogen was also identified. An isogenic mutant defective in this Moraxella Hb-utilization protein (MhuA), 7169 : : mhuA, showed a significant lag during growth in the presence of Hb as the sole iron source. This protein appears to be expressed constitutively, regardless of growth conditions, and a mAb directed to MhuA demonstrated that this protein contains highly conserved, surface-exposed epitopes. Data demonstrating that expression of MhuA may be highly specific to isolates of M. catarrhalis are also presented, suggesting a potential role as a diagnostic marker. To our knowledge, this is the first report demonstrating that M. catarrhalis expresses an Hb-binding protein and that this bacterium can utilize Hb as a sole iron source for growth.
Furano
Kristin
K
Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.|Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Luke
Nicole R
NR
Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.|Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Howlett
Amy J
AJ
Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.|Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Campagnari
Anthony A
AA
Division of Infectious Diseases, Department of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA.|Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.|Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA.
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D045828:Moraxellaceae Infections / Q000382:microbiology; D016296:Mutagenesis; D014774:Virulence
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D000939:Epitopes; D006454:Hemoglobins; D007501:Iron