Embryonic mortality in buffaloes synchronized and mated by AI during the seasonal decline in reproductive function.
The aim was to determine the factors that contribute to embryonic mortality in buffaloes mated by AI during a period of increasing day length which corresponds to a natural decline in reproductive activity. Italian Mediterranean buffalo cows (n=243) showing regular estrous cycles were synchronized using the Ovsynch-TAI program and mated by AI at 16 and 40 h after the second injection of GnRH. Blood samples were collected on Days 10 and 20 after the first AI and assayed for progesterone (P4). Pregnancy diagnosis was undertaken on Days 26 and 40 after the first AI using rectal ultrasonography. Buffaloes with a conceptus on Day 26 but not on Day 40 were judged to have undergone embryonic mortality and for these animals uterine fluid was recovered by flushing and analysed for common infectious agents. Estrus synchronization was achieved in 86% of buffaloes and the pregnancy rate on Day 40 was 34%. Embryonic mortality between Days 26 and 40 occurred in 45% of buffaloes and was associated with the presence of significant infectious agents in only 10 buffaloes (8%). Concentrations of P4 on Day 10 after AI were higher (P<0.05) in buffaloes that established a pregnancy than in buffaloes that showed embryonic mortality that was not associated with infectious agents. Similarly, on Day 20 after AI P4 concentrations were higher (P<0.01) in pregnant buffaloes compared with non-pregnant buffaloes and buffaloes that had embryonic mortality. It is concluded that a reduced capacity for P4 secretion can explain around 50% of embryonic mortalities in buffaloes synchronised and mated by AI during a period of low reproductive activity and that other as yet unidentified factors also have a significant effect on embryonic survival.
Campanile
Giuseppe
G
DISCIZIA, Faculty of Veterinary Medicine, Via F. Delpino 1, Federico II University, 80137 Naples, Italy. giucampa@unina.it
Neglia
Gianluca
G
Gasparrini
Bianca
B
Galiero
Giorgio
G
Prandi
Alberto
A
Di Palo
Rossella
R
D'Occhio
Michael J
MJ
Zicarelli
Luigi
L
D000818:Animals; D002020:Buffaloes / Q000196:embryology / Q000502:physiology*; D003338:Corpus Luteum / Q000502:physiology; D020964:Embryo Loss / Q000453:epidemiology / Q000662:veterinary*; D004973:Estrus Synchronization; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000502:physiology*; D012621:Seasons; D013997:Time Factors
D011374:Progesterone