Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential.
The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.
Lu
Wei
W
Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China.
Wu
Xiao-Dong
XD
Shi
Mu De
MD
Yang
Rui Fu
RF
He
You Yu
YY
Bian
Chao
C
Shi
Tie Liu
TL
Yang
Sheng
S
Zhu
Xue-Liang
XL
Jiang
Wei-Hong
WH
Li
Yi Xue
YX
Yan
Lin-Chen
LC
Ji
Yong Yong
YY
Lin
Ying
Y
Lin
Guo-Mei
GM
Tian
Lin
L
Wang
Jin
J
Wang
Hong Xia
HX
Xie
You Hua
YH
Pei
Gang
G
Wu
Jia Rui
JR
Sun
Bing
B
D000595:Amino Acid Sequence; D015153:Blotting, Western; D019295:Computational Biology; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
D010455:Peptides; D014764:Viral Proteins