High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site.
An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.
Lee
Jin-Ching
JC
Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 128 Yen-Chiu-Yuan Road, Sec. 2, Taipei, Taiwan, Republic of China.
Wu
Tzong-Yuan
TY
Huang
Chien-Fu
CF
Yang
Feng-Mine
FM
Shih
Shin-Ru
SR
Hsu
John T A
JT
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D004686:Encephalomyocarditis virus / Q000235:genetics*; D024861:Genetic Enhancement / Q000379:methods; D006801:Humans; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D011993:Recombinant Fusion Proteins