Characterization of light chain immunoglobulin in urine from animals and humans infected with prion diseases.
The necessity of a non-invasive in-vivo test for prion diseases has become more apparent since the transmission of vCJD from the blood of a healthy individual incubating the disease. Here we show that prion urine comprises an array of protease resistant peptides, among them light chain immunoglobulin (LC). This was observed by sequencing gel bands comprising hamster urine samples, as well as by immunoblotting of similar samples with anti mouse IgG reagents for hamster samples, or with anti human IgG reagents for human samples. Our result suggests that urine samples from CJD patients can be identified by the presence of protease resistant proteins such as LC.
Kariv-Inbal
Zehavit
Z
Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem 91120, Israel.
Halimi
Michele
M
Dayan
Yael
Y
Engelstein
Roni
R
Gabizon
Ruth
R
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001536:Bence Jones Protein / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D007562:Creutzfeldt-Jakob Syndrome / Q000276:immunology* / Q000652:urine*; D006224:Cricetinae; D003956:Dialysis / Q000379:methods; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000187:drug effects / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods; D010447:Peptide Hydrolases / Q000494:pharmacology; D014461:Ultracentrifugation / Q000379:methods
D001425:Bacterial Outer Membrane Proteins; D007147:Immunoglobulin Light Chains; D001536:Bence Jones Protein; D010447:Peptide Hydrolases