Localization of the endothelin-B receptor using a transgenic approach.
The use of reporter genes such as beta-galactosidase and green fluorescent protein is a powerful molecular tool for the visualization of in-vivo gene expression. In this paper, we describe the generation of mice that possess a beta-galactosidase reporter gene introduced into the endothelin-B receptor locus by homologous recombination in embryonic stem cells. These mice express beta- galactosidase wherever endothelin-B is expressed and therefore provide a precise in-vivo localization profile of endothelin-B expression.
Gordon
Adele
A
Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.
Webb
David
D
Shin
Myung
M
Kotelevtsev
Yuri
Y
D000818:Animals; D053595:Embryonic Stem Cells / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors; D007763:Lac Operon; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D044023:Receptor, Endothelin B / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D044023:Receptor, Endothelin B; D001616:beta-Galactosidase