[Immunoprotection induced by recombinant plasmids pVAX1-Mzp5-7 of Eimeria tenella].
OBJECTIVE
To observe the immunoprotection of recombinant plasmids of lambda Mzp5-7 to chicken challenged with Eimeria tenella (E. t) oocysts.


METHODS
All the chickens were immunized with the recombinant plasmids by different inoculation pathways, three times on d7, d14 and d21, and challenged with E. t oocysts. The response of specific humoral and cell immunity were detected by the immunological methods such as the specific antibody response, the ratio of CD4+/CD8+ T cells.


RESULTS
The results showed that the recombinant plasmids can induce immune response. The specific immune responses were strengthened with the increase of immunization times. The difference of the immunity indexes among experiment groups was not significant, while the ratio of CD4+/CD8+ T cells and antibody titer were significantly higher in experiment groups than those in the control groups. The number of oocysts shed in experiment groups decreased and the shedding duration was shorter significantly. The body weight of the chicken in experiment groups increased significantly and the lesion in cecum was slighter than that of control groups.


CONCLUSIONS
The recombinant plasmids of lambda Mzp5-7 show an immunoprotection to chicken challenged with E. t oocysts.
Qin
Rui-ling
RL
Faculty of Military Veterinary Medicine, Quartermaster University of PLA, Changchun 130062, China.
Zhang
Xi-chen
XC
Li
Jian-hua
JH
Xu
Zhan-yun
ZY
Yin
Ji-gang
JG
Yang
Ju
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D016516:CD4-CD8 Ratio; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control*; D016786:Eimeria tenella / Q000276:immunology*; D008297:Male; D010957:Plasmids / Q000276:immunology*; D011995:Recombination, Genetic; D019444:Vaccines, DNA / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D019444:Vaccines, DNA; C061194:Eimeria tenella sporozoite antigen