BLG-e1 - a novel regulatory element in the distal region of the beta-lactoglobulin gene promoter.
beta-Lactoglobulin (BLG) is a major ruminant milk protein. A regulatory element, termed BLG-e1, was defined in the distal region of the ovine BLG gene promoter. This 299-bp element lacks the established cis-regulatory sequences that affect milk-protein gene expression. Nevertheless, it alters the binding of downstream BLG sequences to histone H4 and the sensitivity of the histone-DNA complexes to trichostatin A treatment. In mammary cells cultured under favorable lactogenic conditions, BLG-e1 acts as a potent, position-independent silencer of BLG/luciferase expression, and similarly affects the promoter activity of the mouse whey acidic protein gene. Intragenic sequences upstream of BLG exon 2 reverse the silencing effect of BLG-e1 in vitro and in transgenic mice.
Reichenstein
Moshe
M
Institute of Animal Science, ARO, The Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel.
German
Tania
T
Barash
Itamar
I
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007782:Lactoglobulins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D017931:DNA Primers; D007782:Lactoglobulins