title: c-src activating mutation analysis in Chinese patients with colorectal cancer.
abstract: OBJECTIVE
  To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland.
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases.
  
  
  RESULTS
  Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found.
  
  
  CONCLUSIONS
  c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
authors.affiliation: International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Institute, Shanghai 200438, China.
authors.forename: Ye-Xiong
authors.initials: YX
authors.lastname: Tan
authors.forename: Han-Tao
authors.initials: HT
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Yan
authors.forename: Guan-Long
authors.initials: GL
authors.lastname: Dai
authors.forename: Meng-Chao
authors.initials: MC
authors.lastname: Wu
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016391:Genes, src / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics / Q000556:secondary; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational