title: [Mapping of regulatory domain of T-protein from Escherichia coli].
abstract: To map the regulatory domain of Escherichia coli T-protein.
  
  
  
  Fragmentation cloning was employed in cloning of 11 fragments from T-protein. The regulatory activity of each fragment was determined respectively.
  
  
  
  The regulatory domain of T-protein was located in the C-terminal 270 amino acids, which was the same location as PDH domain.
  
  
  
  T-protein has no independent regulatory domain.
authors.affiliation: Department of Pharmacy, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Shu-qing
authors.initials: SQ
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular; D015253:Deoxyribonucleases, Type I Site-Specific / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D050436:Regulatory Elements, Transcriptional / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D015253:Deoxyribonucleases, Type I Site-Specific; C109830:HsdR protein, E coli