WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of stem cell fate in Arabidopsis.
SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. However, the biological targets controlled by these ATPases are largely unknown. Using genetic and molecular analyses we have identified WUSCHEL (WUS) as a biologically important target of the SNF2-class ATPase SPLAYED (SYD) in the shoot apical meristem of Arabidopsis. We present evidence that SYD is recruited to the WUS promoter and that it is involved in regulation of the stem cell pool maintenance via direct transcriptional control of this master regulator.
Kwon
Chang Seob
CS
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Chen
Changbin
C
Wagner
Doris
D
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D018519:Meristem / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D018520:Plant Shoots / Q000166:cytology; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013234:Stem Cells / Q000166:cytology*
D029681:Arabidopsis Proteins; D017931:DNA Primers; D018398:Homeodomain Proteins; C116421:WUSCHEL protein, Arabidopsis; D005966:Glucuronidase; D000251:Adenosine Triphosphatases; C452429:SPLAYED protein, Arabidopsis