title: Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation.
abstract: Biomaterial surface chemistry has profound consequences on cellular and host responses, but the underlying molecular mechanisms remain poorly understood. Using self-assembled monolayers as model biomaterial surfaces presenting well defined chemistries, we demonstrate that surface chemistry modulates osteoblastic differentiation and matrix mineralization independently from alterations in cell proliferation. Surfaces were precoated with equal densities of fibronectin (FN), and surface chemistry modulated FN structure to alter integrin adhesion receptor binding. OH- and NH(2)-terminated surfaces up-regulated osteoblast-specific gene expression, alkaline phosphatase enzymatic activity, and matrix mineralization compared with surfaces presenting COOH and CH(3) groups. These surface chemistry-dependent differences in cell differentiation were controlled by binding of specific integrins to adsorbed FN. Function-perturbing antibodies against the central cell binding domain of FN completely inhibited matrix mineralization. Furthermore, blocking antibodies against beta(1) integrin inhibited matrix mineralization on the OH and NH(2) surfaces, whereas function-perturbing antibodies specific for beta(3) integrin increased mineralization on the COOH substrate. These results establish surface-dependent differences in integrin binding as a mechanism regulating differential cellular responses to biomaterial surfaces. This mechanism could be exploited to engineer materials that control integrin binding specificity to elicit desired cellular activities to enhance the integration of biomaterials and improve the performance of biotechnological culture supports.
authors.affiliation: Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Keselowsky
authors.forename: David M
authors.initials: DM
authors.lastname: Collard
authors.forename: Andrés J
authors.initials: AJ
authors.lastname: García
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000187:drug effects*; D005353:Fibronectins / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016023:Integrins / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D016023:Integrins