title: Ornithine decarboxylase gene is overexpressed in colorectal carcinoma.
abstract: OBJECTIVE
  To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.
  
  
  METHODS
  Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by RT-PCR. ODC genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting assay.
  
  
  RESULTS
  A cell line, which could steadily secrete anti-ODC mAb, was selected through subcloning four times. Western blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P<0.01). Immunohistochemical staining showed that colorectal carcinoma cells expressed a significantly higher level of ODC than normal colorectal mucosa (98.6+/-1.03% vs 5.26+/-5%, P<0.01).
  
  
  CONCLUSIONS
  ODC gene overexpression is significantly related to human colorectal carcinoma. ODC gene expression may be a marker for the gene diagnosis and therapy of colorectal carcinoma.
authors.affiliation: Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Hu
authors.forename: Xian-Xi
authors.initials: XX
authors.lastname: Liu
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Jiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Shi-Lian
authors.initials: SL
authors.lastname: Liu
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Bian
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Geng
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D009955:Ornithine Decarboxylase