[Preparation of a monoclonal antibodies against Plasmodium falciparum glutamate dehydrogenase and establishment of colloidal gold-immunochromatographic assay].
OBJECTIVE
To prepare a monoclonal antibodies (mAbs) against glutamate dehydrogenase (GDH) of Plasmodium falciparum (FCC1/HN strain) and establish colloidal gold-immunochromatographic assay (GICA) for diagnosis of Plasmodium falciparum malaria.


METHODS
Recombinant GDH was used to immunize Balb/C mice and the mAbs against GDH were prepared using hybridoma technique followed by identification of IgG isotype and its affinity. Protein-G affinity chromatography was employed to purify the antibodies, which were labeled with colloidal gold for establishment of GICA for Plasmodium falciparum detection.


RESULTS
Six mAbs were obtained and identified as IgG1(kappa) of IgG isotypes with affinity constants (Kaff) ranging from 1 x 10(-8) to 2.8 x 10(-10). GICA had a sensitivity of 86.66%; and specificity of 96.43%; for Plasmodium falciparum detection compared with routine microscopic examination.


CONCLUSIONS
The established GICA is rapid and accurate for Plasmodium falciparum detection with such potential utility as for instant diagnosis of Plasmodium falciparum malaria.
Li
Yan
Y
Department of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Ning
Yun-Shan
YS
Li
Li
L
Peng
Dan-Dan
DD
Dong
Wen-Qi
WQ
Li
Ming
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000913:Antibodies, Protozoan / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005969:Glutamate Dehydrogenase / Q000276:immunology*; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D017979:Gold Colloid; D005969:Glutamate Dehydrogenase