Gene expression profiles of HeLa Cells impacted by hepatitis C virus non-structural protein NS4B.
By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RTPCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeleton and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.
Zheng
Yi
Y
Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China.
Ye
Lin-bai
LB
Liu
Jing
J
Jing
Wei
W
Timani
Khalid A
KA
Yang
Xiao-jun
XJ
Yang
Fan
F
Wang
Wei
W
Gao
Bo
B
Wu
Zhen-hui
ZH
D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006367:HeLa Cells; D016174:Hepacivirus / Q000472:pathogenicity*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D017361:Viral Nonstructural Proteins