The immunomodulatory effects of the dimer of lysozyme (KLP-602) in carp (Cyprinus carpio L)--in vivo study.
The dimer of lysozyme was administered to carp (Cyprinus carpio L.) in an attempt to reduce the suppression of response in fish exposed to the cypermethrin. Experimental studies were carried out on cells isolated from blood and pronephros of carp. In the in vivo studies fish were intoxicated with cypermethrin (synthetic pesticide) at a dose of LD5. After 24 h fish were injected intraperitoneally with a modulator-the dimer of lysozyme (KLP-602) at a dose of 0.02 mg/kg of body weight. In the study we determined the metabolic activity of phagocyte cells and the proliferative ability of lymphocytes stimulated by ConA and LPS. The results indicate that this modulator is useful for stimulation of cellular and humoral immunity after experimentally induced suppression by selected pesticide.
Rymuszka
Anna
A
Department of Physiology and Toxicology, Catholic University of Lublin, 14 Al. Racławickie Str., 20-950 Lubin, Poland. anrym@kul.lublin.pl
Studnicka
Maria
M
Siwicki
Andrzej K
AK
Sierosławska
Anna
A
Bownik
Adam
A
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002347:Carps / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D007111:Immunity, Cellular / Q000187:drug effects*; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D007306:Insecticides / Q000633:toxicity; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D009113:Muramidase / Q000008:administration & dosage / Q000494:pharmacology*; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity; D016897:Respiratory Burst / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D007155:Immunologic Factors; D007306:Insecticides; D008934:Mitogens; D011722:Pyrethrins; D013778:Tetrazolium Salts; D013844:Thiazoles; C017160:cypermethrin; C488571:KLP602; D009113:Muramidase; C022616:thiazolyl blue