title: Keratin 19 gene drives Cre recombinase expression throughout the early postimplantation mouse embryo.
abstract: The development of Cre-lox technology has created new opportunities for studying the tissue-specific functions of genes in vivo during development and disease. We analyzed the spatial and temporal activity of Cre recombinase whose coding sequence was inserted into the endogenous locus for keratin 19. Rather than providing epithelial-specific recombination during organogenesis, this K19cre allele allows unexpected recombination in early embryonic development, resulting in recombination of a loxP-flanked allele throughout all tissues of the mouse, but with sparing of the extraembryonic endoderm, including the anterior visceral endoderm.
authors.affiliation: Department of Surgical Sciences, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2733, USA. anna.means@vanderbilt.edu
authors.forename: Anna L
authors.initials: AL
authors.lastname: Means
authors.forename: Anna
authors.initials: A
authors.lastname: Chytil
authors.forename: Harold L
authors.initials: HL
authors.lastname: Moses
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Coffey
authors.forename: Christopher V E
authors.initials: CV
authors.lastname: Wright
authors.forename: Makoto M
authors.initials: MM
authors.lastname: Taketo
authors.forename: William M
authors.initials: WM
authors.lastname: Grady
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019426:Integrases / Q000096:biosynthesis*; D007633:Keratins / Q000235:genetics* / Q000502:physiology*; D008297:Male; D051379:Mice; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D007633:Keratins; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase