Liquid chromatography-mass spectrometric and liquid chromatography-tandem mass spectrometric determination of hallucinogenic indoles psilocin and psilocybin in "magic mushroom" samples.
Accurate and sensitive analytical methods for psilocin (PC) and psilocybin (PB), tryptamine-type hallucinogens contained in "magic mushrooms," were investigated using liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS-MS). The chromatographic separation on an ODS column and mass spectral information gave complete discrimination between PC and PB without derivatization. The mass spectrometric detection had a high sensitivity, and the tandem mass spectrometric detection provided more specificity and accuracy, as well as high sensitivity. The detection limits ranged from 1 to 25 pg by LC-MS in the selected ion monitoring mode, and the intra- and inter-day coefficients of variation were estimated to be 4.21-5.93% by LC-MS-MS in the selected reaction monitoring mode. By applying the present LC-MS-MS technique to four real samples, the contents of PC and PB were found to vary over a wide range (0.60-1.4 and 0.18-3.8 mg/g dry wt. for PC and PB, respectively) between samples.
Kamata
Tooru
T
Forensic Science Laboratory, Osaka Prefectural Police H.Q., 1-3-18, Hommachi, Chuo-ku, Osaka 541-0053, Japan. t-kamata@mahoroba.ne.jp
Nishikawa
Mayumi
M
Katagi
Munehiro
M
Tsuchihashi
Hitoshi
H
D000363:Agaricales / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006213:Hallucinogens / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D011562:Psilocybin / Q000031:analogs & derivatives* / Q000032:analysis*; D012680:Sensitivity and Specificity
D006213:Hallucinogens; D011562:Psilocybin; C009105:psilocin