title: [Determination of levodropropizine and its pharmacokinetics in human plasma using LC/MS/MS].
abstract: OBJECTIVE
  To develop a rapid and sensitive LC/MS/MS method for the analysis of levodropropizine in plasma and study the pharmacokinetics of levodropropizine in healthy Chinese volunteers.
  
  
  METHODS
  Levodropropizine and zolmitriptan (internal standard, IS) were extracted from plasma samples and chromatographed on a C18 column and detected using a tandem mass spectrometer with a TurboIon Spray ionization interface. Quantitation was performed using multiple reaction monitoring (MRM) of the transitions of the m/z 237 --> m/z 120 for levodropropizine and m/z 288 --> m/z 58 for the IS.
  
  
  RESULTS
  The limit of quantification of the method for levodropropizine was 0.25 microg x L(-1). The assay was linear over the concentration range from 0.25 to 500.0 microg x L(-1) and intra- and inter-day precision over this range were < 11.4% with good accuracy.
  
  
  CONCLUSIONS
  The method is shown to be accurate, and suitable for clinical pharmacokinetic study of levodropropizine.
authors.affiliation: Laboratory of Clinical Pharmacology, No. 2 Hospital of China Medical University, Shenyang 110004, China. LMZhao16@hotmail.com
authors.forename: Li-mei
authors.initials: LM
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Ya-xin
authors.initials: YX
authors.lastname: Sun
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Shan-bin
authors.initials: SB
authors.lastname: Guo
mesh_terms: D000284:Administration, Oral; D000996:Antitussive Agents / Q000097:blood / Q000493:pharmacokinetics; D019540:Area Under Curve; D002853:Chromatography, Liquid; D006801:Humans; D008297:Male; D011409:Propylene Glycols / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000996:Antitussive Agents; D011409:Propylene Glycols; C035916:dipropizine