Effects of cyclic tensile forces on the expression of vascular endothelial growth factor (VEGF) and macrophage-colony-stimulating factor (M-CSF) in murine osteoblastic MC3T3-E1 cells.
It has been reported that vascular endothelial growth factor (VEGF), expressed by osteoblasts, can induce osteoclast recruitment and thus affects bone remodeling. The purpose of this study was to investigate the effects of cyclic tensile forces on the expression of VEGF and macrophage-colony-stimulating factor (M-CSF) in osteoblastic MC3T3-E1 cells. VEGF and M-CSF gene expression and protein concentration were determined by real-time PCR and enzyme-linked immunoassay. The expression of VEGF and M-CSF mRNA in the experimental group was higher than in the control group. The increase in the concentration of VEGF and M-CSF protein in the experimental group was time-dependent. Moreover, gadolinium (an S-A channel inhibitor), but not nifedipine (L-Type Ca2+ channel blocker), treatment reduced the concentration of VEGF and M-CSF mRNA and protein in the experimental groups. These findings suggest that cyclic tensile forces increase the expression of VEGF and M-CSF in osteoblastic MC3T3-E1 cells via a stretch-activated channel (S-A channel).
Motokawa
M
M
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan. motumo@hiroshima-u.ac.jp
Kaku
M
M
Tohma
Y
Y
Kawata
T
T
Fujita
T
T
Kohno
S
S
Tsutsui
K
K
Ohtani
J
J
Tenjo
K
K
Shigekawa
M
M
Kamada
H
H
Tanne
K
K
D016475:3T3 Cells; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D005682:Gadolinium / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000235:genetics; D007473:Ion Channels / Q000037:antagonists & inhibitors; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis* / Q000235:genetics; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D010006:Osteoblasts / Q000378:metabolism*; D013314:Stress, Mechanical; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
D002121:Calcium Channel Blockers; D007473:Ion Channels; D042461:Vascular Endothelial Growth Factor A; D016173:Macrophage Colony-Stimulating Factor; D005682:Gadolinium; D009543:Nifedipine