title: GC/MS identification and quantification of constituents of bacterial lipids and glycoconjugates obtained after methanolysis as heptafluorobutyrate derivatives.
abstract: In previous articles [Anal. Biochem. 284 (2000) 201; J. Lipid Res. 43 (2002) 794], we reported that the GC/MS identification and quantification of nearly all constituents of glycolipids could be obtained on the same sample in a single GC/MS analysis as heptafluorobutyrate derivatives of the products liberated using acid-catalyzed methanolysis. The same type of data could be obtained on glycoproteins and proteoglycans [Biochemistry 42 (2003) 8342]. These experiments were performed on material from higher organisms, and there was no evidence that bacteria-specific constituents could also be identified and quantified. The current article reports that the GC/MS analysis of compounds liberated by acid-catalyzed methanolysis as heptafluorobutyrate derivatives allows the simultaneous qualitative and quantitative determinations of pentoses, deoxyhexoses, hexoses, hexosamines, uronic acids, Kdo, Mur, heptose, Kdn, and neuraminic acid as well as of most fatty acids (including hydroxylated fatty acids). This approach provides a way of obtaining fingerprints of bacterial constituents and quantification of the overall effect of gene inactivation or of culture conditions.
authors.affiliation: CNRS Unité Mixte de Recherche 8576, Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille Bâtiment C9, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bohin
authors.forename: Franck
authors.initials: F
authors.lastname: Bouchart
authors.forename: Colette
authors.initials: C
authors.lastname: Richet
authors.forename: Osarath
authors.initials: O
authors.lastname: Kol
authors.forename: Yves
authors.initials: Y
authors.lastname: Leroy
authors.forename: Philippe
authors.initials: P
authors.lastname: Timmerman
authors.forename: Guillemette
authors.initials: G
authors.lastname: Huet
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Bohin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Zanetta
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification; D005466:Fluorocarbons / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006001:Glycoconjugates / Q000302:isolation & purification*; D006880:Hydroxy Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D009005:Monosaccharides / Q000302:isolation & purification; D009112:Muramic Acids / Q000032:analysis; D011073:Polyamines / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D005227:Fatty Acids; D005466:Fluorocarbons; D006001:Glycoconjugates; D006880:Hydroxy Acids; D008055:Lipids; D009005:Monosaccharides; D009112:Muramic Acids; D011073:Polyamines; C007924:heptafluorobutyric anhydride; D000432:Methanol