In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract.
Recent reports have suggested that mesenchymal cells derived from bone marrow may differentiate into not only mesenchymal lineage cells but also other lineage cells. There is possibility for insulin-producing cells (IPCs) to be differentiated from mesenchymal cells. We used self-functional repair stimuli of stem cells by partial injury. Rat pancreatic extract (RPE) from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to rat mesenchymal cells. After the treatment of RPE, they made clusters like islet of Langerhans within a week and expressed four pancreatic endocrine hormones; insulin, glucagon, pancreatic polypeptide, and somatostatin. Moreover, IPCs released insulin in response to normal glucose challenge. Here we demonstrate that the treatment of RPE can differentiate rat mesenchymal cells into IPCs which can be a potential source for the therapy of diabetes.
Choi
Kyung Suk
KS
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Shin
Jun-Seop
JS
Lee
Jae-Jeong
JJ
Kim
Young Soo
YS
Kim
Seung-Bum
SB
Kim
Chan-Wha
CW
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005947:Glucose / Q000502:physiology; D051273:Glucose Transporter Type 2; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology*
D051273:Glucose Transporter Type 2; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D014020:Tissue Extracts; D005947:Glucose