Genotoxicity of antimicrobial endodontic compounds by single cell gel (comet) assay in Chinese hamster ovary (CHO) cells.
OBJECTIVE
In the current study, the potential DNA damage associated with exposure to a number of antimicrobial endodontic compounds was assessed by the single cell gel (comet) assay in vitro.


METHODS
Chinese hamster ovary (CHO) cells were exposed to formocresol, paramonochlorophenol, calcium hydroxide, or chlorhexidine at final concentration ranging from 0.01% to 1%.


RESULTS
Formocresol, paramonochlorophenol, and calcium hydroxide, as well as chlorhexidine in all concentrations tested did not contribute to the DNA damage.


CONCLUSIONS
These findings are clinically relevant since they represent an important contribution to the correct evaluation of the potential health risk associated with exposure to dental agents.
Ribeiro
Daniel Araki
DA
Department of Pathology, Botucatu Medical School -- São Paulo State University, Botucatu, Brazil. ak92@hotmail.com
Scolastici
Clarissa
C
De Lima
Patricia Lepage Alves
PL
Marques
Mariangela Esther Alencar
ME
Salvadori
Daisy Maria Favero
DM
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D016466:CHO Cells / Q000187:drug effects; D002126:Calcium Hydroxide / Q000633:toxicity; D002710:Chlorhexidine / Q000633:toxicity; D002733:Chlorophenols / Q000633:toxicity; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D005566:Formocresols / Q000633:toxicity; D012388:Root Canal Irrigants / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D005566:Formocresols; D012388:Root Canal Irrigants; C031025:formocresol; C029107:4-chlorophenol; D002126:Calcium Hydroxide; D002710:Chlorhexidine