title: First outbreak of respiratory disease associated with swine influenza H1N2 virus in pigs in Korea.
abstract: This report describes the first diagnosis of swine influenza H1N2 virus infection in growing pigs in Korea by virus isolation, reverse transcription-polymerase chain reaction (RT-PCR), histopathology, and in situ hybridization. The subtype of swine influenza virus isolates was determined to be H1N2 by RT-PCR. The most consistent and predominant histological feature was bronchointerstitial pneumonia. Lung tissues from these pigs were hybridized with the nonradioactive digoxigenin-labeled complementary DNA (cDNA) probe from H1 HA and N2 NA genes but not from H3 HA and N1 NA genes. A strong hybridization signal was seen in bronchial- and bronchiolar-lining epithelial cells and in alveolar and interstitial macrophages.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
authors.forename: Kwonil
authors.initials: K
authors.lastname: Jung
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D001980:Bronchi / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007723:Korea / Q000453:epidemiology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*
chemical_list: D012367:RNA, Viral