Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media.
Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.
Pao
Steven
S
Virginia State University, Agricultural Research Station, P.O. Box 9061, Petersburg, VA 23806, USA. spao@vsu.edu
Patel
Dhartika
D
Kalantari
Aref
A
Tritschler
Joseph P
JP
Wildeus
Stephan
S
Sayre
Brian L
BL
D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D004927:Escherichia coli Infections / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D015511:Goat Diseases / Q000382:microbiology; D006041:Goats; D007124:Immunoenzyme Techniques; D008297:Male; D012418:Ruminants / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D014768:Virginia
D003470:Culture Media