Bioactivity quantification of crude bacteriocin solutions.
Bacteriocin activity can be quantified by a simple parallel line model based on an agar diffusion assay. The quotient between the inhibition zone area and the sensitivity of the indicator bacterium can be used to compare different bacteriocins. Deferred calculations of relative potencies are possible, once purified compounds are obtained.
Delgado
Amélia
A
Instituto de Tecnologia Química e Biológica, Department de Microbiology, Av. da República, Quinta do Marquês 2780-505 Oeiras, Portugal. ameliad@itqb.unl.pt
Brito
Dulce
D
Fevereiro
Pedro
P
Tenreiro
Rogério
R
Peres
Cidália
C
D001430:Bacteriocins / Q000494:pharmacology*; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development; D007934:Leuconostoc / Q000187:drug effects* / Q000254:growth & development; D009561:Nisin / Q000494:pharmacology*
D001430:Bacteriocins; D009561:Nisin