title: [In vivo effect of endometria's and human cervix's secretions on the spermatozoid].
abstract: OBJECTIVE
  To demonstrate the capacitance stages acquired by the spermatozoa after the in vivo contact with the endometrium and the human cervical canal.
  
  
  METHODS
  Experimental, prospective, and longitudinal.
  
  
  METHODS
  Spermatozoa obtained from the cervix and endometrium, plus post-capacitated semen in a 1 and 4 hours process, from couples that have proved fertility, were processed to evaluate motility, morphology, vitality and identification of morphological patterns with chlortetracycline and then inducted to acrosomal reaction with ionophore A23187.
  
  
  RESULTS
  A safe, fast and harmless technique to obtain endometrial secretions is described. The spermatozoa capacitated in vitro and in vivo developed specific capacitance stages, applying Perry's patterns with the CTC test. Spermatozoa capacitated in vitro and then recovered in the cervix responded favorably to the induction of the acrosomal reaction with calcium ionophore A23187, differing from the obtained with endometrium wash.
  
  
  CONCLUSIONS
  The spermatozoa recovered from the endometrium presented a more advanced capacitance stage than that obtained from the cervix, this is demonstrated with the induction of the acrosomal reaction and the CTC test. These findings suggest that endometrial and cervical tissues promote capacitance, different in each one of them and progressive in their biological route.
authors.affiliation: Departamento de Ginecología y Obstetricia, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, NL. merino_ruiz@yahoo.com.mx
authors.forename: Martha
authors.initials: M
authors.lastname: Merino Ruiz
authors.forename: Hilda Magdalena
authors.initials: HM
authors.lastname: Luna Aranda
authors.forename: Rogelio
authors.initials: R
authors.lastname: García Flores
authors.forename: Oscar
authors.initials: O
authors.lastname: Vidal Gutiérrez
authors.forename: Felipe Arturo
authors.initials: FA
authors.lastname: Morales Martínez
authors.forename: Luis
authors.initials: L
authors.lastname: Sordia Hernández
authors.forename: Donato
authors.initials: D
authors.lastname: Saldívar Rodríguez
authors.forename: Juana
authors.initials: J
authors.lastname: Vázquez Méndez
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000378:metabolism* / Q000502:physiology; D004717:Endometrium / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D011446:Prospective Studies; D013075:Sperm Capacitation / Q000502:physiology*