Synthesis and bioactivities of two multiple antigen peptides as potential vaccine against schistosoma.
Based on the two antigenic peptides, 26-43 (P26) and 116-131 (P116), derived from 28 kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST), two multiple antigenic peptides (MAPs), (P26)4-MAP and (P116)4-MAP with the same oligomeric lysine core, were synthesized by stepwise solid-phase peptide synthesis method. The antigenicities and protective effects of these two MAPs were examined on experimental animals. As shown in the dot-ELISA result, the synthetic MAPs could be recognized and bound by immunoglobins in both patient's and infected-rabbit's sera. After Kunming mice were immunized with (P26)4-MAP, the worm burden reduction rate and the liver egg reduction rate were 59.9% and 61.1%. In (P26)4-MAP or (P116)4-MAP immunized BALB/c mice, the worm burden reduction rates were 37.5% and 62.5%, respectively, and the liver egg reduction rates were 35.1% and 54.0%, respectively.
Huang
He-Qing
HQ
Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China.
Li
Shu-Chun
SC
Qin
Zhi-Hui
ZH
Cao
Sheng-Li
SL
Yao
Yun
Y
Liu
Yu-Shi
YS
Li
Huai-Yu
HY
Cai
Meng-Shen
MS
Li
Zhong-Jun
ZJ
Shi
You-En
YE
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000201:enzymology / Q000382:microbiology; D012555:Schistosomiasis mansoni / Q000276:immunology*; D014612:Vaccines
D000947:Antigens, Helminth; D010455:Peptides; D014612:Vaccines; D005982:Glutathione Transferase