title: Induction of Gag-specific T-cell responses by therapeutic immunization with a Gag-expressing Sendai virus vector in macaques chronically infected with simian-human immunodeficiency virus.
abstract: Recent prophylactic vaccine trials inducing virus-specific CD8+ T-cell responses have shown control of primary infections of a pathogenic simian-human immunodeficiency virus (SHIV) in macaques. In the chronic phase, therapeutic immunization replenishing virus-specific CD8+ T-cells is likely to contribute to sustained control of virus replication. In this study, we have administered a recombinant Sendai virus (SeV) vector into five rhesus macaques that had received prophylactic vaccinations and had controlled SHIV replication for more than 1 year after challenge. Our results indicate that virus-specific CD8+ T-cell responses can be expanded and broadened by therapeutic immunization with SeV vectors in the chronic phase after prophylactic vaccine-based control of primary immunodeficiency virus infections.
authors.affiliation: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Moriaki
authors.initials: M
authors.lastname: Kato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Igarashi
authors.forename: Akiko
authors.initials: A
authors.lastname: Takeda
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Munehide
authors.initials: M
authors.lastname: Kano
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Akihiro
authors.initials: A
authors.lastname: Iida
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horie
authors.forename: Eiji
authors.initials: E
authors.lastname: Higashihara
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagai
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matano
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000276:immunology; D015683:Gene Products, gag / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D018655:Lymphocyte Count; D008253:Macaca mulatta; D008297:Male; D009000:Monocytes / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D029082:Sendai virus / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology*; D019562:Viral Load
chemical_list: D015683:Gene Products, gag; D012367:RNA, Viral; D004247:DNA