Platelet activation can occur by shear stress alone in the PFA-100 platelet analyser.
The PFA-100 platelet function analyser simulates high-shear platelet function; however, the relative importance of the high shear rates alone has not been examined in this system. Normal blood was analysed by the PFA-100 using modified cartridges that contained a blank membrane lacking the usual platelet agonists (collagen/epinephrine or collagen/ADP) thus isolating the effects of shear stress alone. We found evidence of platelet activation with significant increases in platelet-leucocyte aggregates. There was no significant change in surface CD62P nor PAC-1 expression. A degree of platelet activation occurs in the PFA-100 due to shear stress alone.
Dunkley
Scott
S
Department of Haematology, Prince of Wales Hospital, Level 4 Campus Centre, Barker St, Randwick, 2031, Sydney, Australia. dunkley@sesahs.nsw.gov.au
Harrison
Paul
P
D001792:Blood Platelets / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis; D010974:Platelet Aggregation / Q000502:physiology*; D010979:Platelet Function Tests; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D013314:Stress, Mechanical
D019007:P-Selectin; D011956:Receptors, Cell Surface; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide