[Purification of a small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus and its inhibition of platelet aggregation].
OBJECTIVE
To investigate the inhibitory effect of the small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus on platelet aggregation.


METHODS
The small peptide SV-PP-3 was isolated and purified by centrifugation at low temperature, sephadex G-75 column chromatography and reverse-phase (HPLC) on C-18 column. The platelet aggregation induced by adenosine diphosphate (ADP) was measured by LBY-NJ aggregometer.


RESULTS
SV-PP-3 was purified from the venom of Agkistrodon blomhoffii siniticus, which inhibited the platelet ADP-induced platelet aggregation in a dose-dependent manner.


CONCLUSIONS
SV-PP-3 can inhibit ADP-Induced platelet aggregation.
Xu
Wei
W
Institute of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. xuwei@fimmu.com
Wu
Shao-Yu
SY
Zhang
Jia-Jie
JJ
Li
Zhi-Qin
ZQ
Wu
Shu-Guang
SG
D017836:Agkistrodon; D000818:Animals; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D012910:Snake Venoms / Q000737:chemistry*
D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D012910:Snake Venoms