title: Envelope glycoproteins are dispensable for insertion of host HLA-DR molecules within nascent human immunodeficiency virus type 1 particles.
abstract: HLA-DR is a host-derived protein present at the surface of HIV-1. To clarify the mechanism through which this molecule is inserted within viruses, we monitored whether the incorporation process might be influenced by the level of virus-encoded envelope (Env) glycoproteins. Wild-type virions and viruses either lacking or bearing lower levels of Env were produced in different cell types. Results from a virus capture test indicate that HLA-DR is efficiently incorporated and at comparable levels in the tested virus preparations. Therefore, Env does not play an active role in the acquisition of host HLA-DR by emerging HIV-1 particles.
authors.affiliation: Laboratory of Human Immuno-Retrovirology, Research Center in Infectious Diseases, RC709, CHUL Research Center, 2705 Laurier Boulevard, Quebec, Canada G1V 4G2.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Martin
authors.forename: Yannick
authors.initials: Y
authors.lastname: Beauséjour
authors.forename: Jacques
authors.initials: J
authors.lastname: Thibodeau
authors.forename: Michel J
authors.initials: MJ
authors.lastname: Tremblay
mesh_terms: D002460:Cell Line; D016655:HIV Core Protein p24 / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D006684:HLA-DR Antigens