title: Validation of a method to replace frozen section during parathyroid exploration by using the rapid parathyroid hormone assay on parathyroid aspirates.
abstract: OBJECTIVE
  The parathyroid hormone (PTH) content of tissue aspirates is an accurate indicator of parathyroid tissue and can replace frozen section during parathyroid surgery.
  
  
  METHODS
  Prospective data collection in a tertiary care hospital with a single surgeon.
  
  
  METHODS
  One hundred sixty-seven consecutive patients completing limited parathyroid explorations.
  
  
  METHODS
  Parathyroid adenomas removed during limited parathyroid exploration were aspirated through a 22-gauge needle into 0.5 mL isotonic sodium chloride solution and the solution held on ice in a purple-top tube. Aspirates of in situ thyroid tissue were also taken for comparison. Samples were then assessed to monitor the physiologic impact of the surgery.
  
  
  METHODS
  The PTH content of tissue aspirates was compared with histologic identification of removed putative parathyroid tissue.
  
  
  RESULTS
  Elevated tissue PTH content was associated with the identification of origin as parathyroid in every case. Tissue aspirates from pathologically proven parathyroid tissue had a mean PTH level of at least 1691 pg/mL, with 160 having values exceeding the upper limit of the assay. This measure was significantly higher than values obtained from thyroid aspirates, which had a mean PTH level of 88 pg/mL (P<.01), reflective of blood levels at the time of aspiration. Using the 99% confidence interval of histologically confirmed parathyroid glands as the lower limit of a positive test result at 1610 pg/mL, tissue aspirate PTH assay has a sensitivity of 97% and a specificity of 100%.
  
  
  CONCLUSIONS
  Positive identification of removed tissue as parathyroid is necessary adjunct to limited parathyroid exploration, where decreases in false positive blood PTH levels can be a result of operative manipulation of the neck. Analysis of tissue PTH content during the same assay that is being used for assessing blood PTH concentration is an efficient and accurate method for identifying the tissue with certainty. This measure also prevents the occasional ambiguity in frozen sections of parathyroid tissue that apparently contain thyroidlike colloid material.
authors.affiliation: Department of Surgery, Brigham Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Rodney K
authors.initials: RK
authors.lastname: Chan
authors.forename: Shahrul I
authors.initials: SI
authors.lastname: Ibrahim
authors.forename: Peter
authors.initials: P
authors.lastname: Pil
authors.forename: Milenko
authors.initials: M
authors.lastname: Tanasijevic
authors.forename: Francis D
authors.initials: FD
authors.lastname: Moore
mesh_terms: D000236:Adenoma / Q000473:pathology* / Q000601:surgery*; D000704:Analysis of Variance; D001707:Biopsy, Needle / Q000379:methods*; D015925:Cryopreservation; D005260:Female; D005629:Frozen Sections; D006801:Humans; D008297:Male; D008875:Middle Aged; D016343:Monitoring, Intraoperative / Q000379:methods; D010281:Parathyroid Hormone / Q000032:analysis*; D010282:Parathyroid Neoplasms / Q000473:pathology* / Q000601:surgery*; D016105:Parathyroidectomy / Q000379:methods; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D010281:Parathyroid Hormone