Determination of surface charge properties of PC-12 cells by electrophoresis.
The electrophoretic behavior of pheochromocytoma (PC-12) cells was investigated both experimentally and theoretically. Cell mobility in aqueous media at different pHs and ionic concentrations was measured, and a model, which assumed that the cell surface contains both acidic and basic functional groups, was proposed. As a result, it was revealed that the experimental data gathered can be described satisfactorily by assuming that the cell surface contains two types of monovalent acidic functional groups and one basic functional group. The values of the dissociation constants of the acidic and basic groups are found to be close to those of acidic amino acids, which indicates that the acidic amino acids may play an important role in the surface electrical properties of PC-12 cells.
Young
Tai-Horng
TH
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan 100, Republic of China. thyoung@ha.mc.ntu.edu.tw
Hung
Chih-Huang
CH
Huang
Shih-Wei
SW
Hsieh
Tien-Shing
TS
Hsu
Jyh-Ping
JP
D000818:Animals; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D016716:PC12 Cells; D051381:Rats; D013499:Surface Properties