title: RNA duplexes with biphenyl substituents as base replacements are less stable than DNA duplexes.
abstract: Introduction of a hydrophobic biphenyl-C-nucleotide pair into a 11-mer RNA duplex is associated with a net penalty in the free energy of duplex formation of 2.0 kcal mol(-1) or 10 degrees C in Tm, relative to DNA. These differential stabilities are of relevance with respect to the transcriptional and translational aspects of hydrophobic base-pairs.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
authors.forename: Christine
authors.initials: C
authors.lastname: Brotschi
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Leumann
mesh_terms: D020029:Base Pairing; D001713:Biphenyl Compounds / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D044366:Transition Temperature / Q000528:radiation effects
chemical_list: D001713:Biphenyl Compounds; D004247:DNA; D012313:RNA; C010574:biphenyl