Modified multiplex PCR for identification of Bacillus Calmette-Guérin substrain Tokyo among clinical isolates.
When an adverse reaction occurs and a mycobacterial species is isolated from a person vaccinated with Bacillus Calmette-Guérin (BCG) or a patient receiving BCG immunotherapy, it is essential to identify whether the isolate is BCG or another mycobacterial species. However, differentiation of BCG from other members of Mycobacterium tuberculosis complex has been very difficult. Using several specific primer-pairs, Bedwell et al. [Bedwell J, Kairo SK, Behr MA, Bygraves JA. Identification of substrains of BCG vaccine using multiplex PCR. Vaccine 2001; 19: 2146-51] recently reported that they could distinguish BCG substrains. We modified their method to improve differentiation of Tokyo 172 from other members of the M. tuberculosis complex, and examined whether this modified method could be applied to clinical isolates. Our method clearly identified BCG substrain (BCG Tokyo 172) among clinical isolates and easily distinguished between M. tuberculosis and wild-type Mycobacterium bovis.
Seki
Masaaki
M
Japan BCG Laboratory, 3-1-5 Matsuyama, Kiyose City, Tokyo 204-0022, Japan. seki@bcg.gr.jp
Sato
Akimasa
A
Honda
Ikuro
I
Yamazaki
Toshio
T
Yano
Ikuya
I
Koyama
Akira
A
Toida
Ichiro
I
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D001500:BCG Vaccine / Q000008:administration & dosage / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D007167:Immunotherapy; D007267:Injections; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000235:genetics; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014041:Tokyo; D001743:Urinary Bladder; D001749:Urinary Bladder Neoplasms / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D004269:DNA, Bacterial