title: TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs.
abstract: Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an approximately 700-bp integration window upstream of tRNA genes with a periodicity of approximately 80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.
authors.affiliation: The Johns Hopkins University School of Medicine, Department of Molecular Biology and Genetics, Baltimore, Maryland 21205, USA.
authors.forename: Nurjana
authors.initials: N
authors.lastname: Bachman
authors.forename: Marnie E
authors.initials: ME
authors.lastname: Gelbart
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsukiyama
authors.forename: Jef D
authors.initials: JD
authors.lastname: Boeke
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013045:Species Specificity; D035361:Transcription Factor TFIIIB / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C474805:Bdp1 protein, S cerevisiae; D017931:DNA Primers; C086468:ISWI protein; D009707:Nucleosomes; D021122:Protein Subunits; D018626:Retroelements; D029701:Saccharomyces cerevisiae Proteins; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors; D012343:RNA, Transfer; D000251:Adenosine Triphosphatases