Comparison of the chemical composition of extracts from Scutellaria lateriflora using accelerated solvent extraction and supercritical fluid extraction versus standard hot water or 70% ethanol extraction.
The aqueous extract of American skullcap (Scutellaria lateriflora L. (S. lateriflora), Lamiaceae) has been traditionally used by North American Indians as a nerve tonic and for its sedative and diuretic properties. Recent reports stated that flavonoids and possibly amino acids are responsible for the anxiolytic activity. As a part of our search for environmentally friendly solvents to extract the active components from medicinal plants, we used S. lateriflora in a comparison of accelerated solvent extraction (ASE) using water, and supercritical fluid extraction (SFE) using CO2 and 10% EtOH as modifier, at different temperatures. Flavonoids and amino acids were quantified by HPLC-UV and HPLC-MS, respectively. The flavonoid content was compared with conventional extraction methods (hot water extraction and 70% ethanol). The use of ASE at 85 degrees C with water as solvent gave the best results for flavonoid glycosides and amino acids, whereas SFE gave higher yields of flavonoid aglycones. However, the results obtained for total flavonoids were not significatively superior to hot water extraction or 70% aqueous EtOH extract.
Bergeron
Chantal
C
Tom's of Maine, P. O. Box 710, Kennebunk, Maine 04043, USA. chantalb@tomsofmaine.com
Gafner
Stefan
S
Clausen
Edgar
E
Carrier
Danielle J
DJ
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D031363:Scutellaria / Q000737:chemistry*; D012997:Solvents; D014867:Water
D000596:Amino Acids; D005419:Flavonoids; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol