title: Overexpression of human copper/zinc-superoxide dismutase in transgenic animals attenuates the reduction of apurinic/apyrimidinic endonuclease expression in neurons after in vitro ischemia and after transient global cerebral ischemia.
abstract: Oxidative stress after ischemia/reperfusion has been shown to induce DNA damage and subsequent DNA repair activity. Apurinic/apyrimidinic endonuclease (APE) is a multifunctional protein in the DNA base excision repair pathway which repairs apurinic/apyrimidinic sites in DNA. We investigated the involvement of oxidative stress and expression of APE in neurons after oxygen-glucose deprivation and after global cerebral ischemia. Our results suggest that overexpression of human copper/zinc-superoxide dismutase reduced oxidative stress with a subsequent decrease in APE expression. Production of oxygen free radicals and inhibition of the base excision repair pathway may play pivotal roles in the cell death pathway after ischemia.
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Stanford, California 943005, USA.
authors.forename: Purnima
authors.initials: P
authors.lastname: Narasimhan
authors.forename: Taku
authors.initials: T
authors.lastname: Sugawara
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Noshita
authors.forename: Pak H
authors.initials: PH
authors.lastname: Chan
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology / Q000235:genetics; D002478:Cells, Cultured; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D013482:Superoxide Dismutase; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase