Teicoplanin-dependent antibodies: detection and characterization.
There are only a few reports of thrombocytopenia associated with clinical doses of teicoplanin, a glycopeptide antibiotic used against Gram-positive bacteria. We investigated 39 patients receiving teicoplanin; 31 were thrombocytopenic with platelet counts between 1-105 x 10(9)/l and 8 were not thrombocytopenic. We identified 14 thrombocytopenic cases (45%) and two (25%) non-thrombocytopenic cases with IgG teicoplanin-dependent platelet-reactive antibodies. Use of glycoprotein (GP) capture enzyme-linked immunosorbent assay with platelets and GPIIb/IIIa transfected Chinese Hamster Ovary cells as well as flow cytometry with GP-deficient platelets indicated that the GPIIb/IIIa complex is a major target antigen of these antibodies.
Garner
Stephen F
SF
National Blood Service, Cambridge, UK. sfg21@cam.ac.uk
Campbell
Kate
K
Smith
Graham
G
Hurd
Colin
C
Davidson
Simon J
SJ
Treacy
Marilyn
M
Burman
John F
JF
Kroll
Hartmut
H
Ouwehand
Willem H
WH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000906:Antibodies / Q000097:blood*; D000937:Antigen-Antibody Reactions; D001792:Blood Platelets / Q000276:immunology; D016466:CHO Cells; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D017334:Teicoplanin / Q000276:immunology*; D013921:Thrombocytopenia / Q000139:chemically induced*
D000900:Anti-Bacterial Agents; D000906:Antibodies; D007074:Immunoglobulin G; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D017334:Teicoplanin