title: Preparation of mono- and diacylglycerols by enzymatic esterification of glycerol with conjugated linoleic acid in hexane.
abstract: Esterification of glycerol with conjugated linoleic acid (CLA) was carried out in hexane. Lipase from Rhizomucor miehei provided a high degree of esterification (80%) in 8 h at 50 degrees C when used at 15% (w/w) in a system containing a 1:2 molar ratio of glycerol to free fatty acids. Esterification levels >80% were obtained in 8 h at 40 degrees C with 15% (w/w) lipase from Candida antarctica at the same molar ratio of reactants. The extent of esterification of CLA was >90% after 4 h of reaction at 50 degrees C with a 5% (w/w) loading of either R. miehei or C. antarctica lipase, together with a 1:1 molar ratio of substrates. Both enzymes incorporated the original CLA as acylglycerol residues in primarily 1,3-diacylglycerol and 1-monoacylglycerol. The CLA-rich acylglycerols can be employed as emulsifiers or as substitutes for natural fats and oils.
authors.affiliation: Coordinación de Posgrado e Investigación, Instituto Tecnológico de Tuxtepec, Tuxtepec, Oax. 68300, Calz. Dr. Víctor Bravo Ahuja s/n, Mexico.
authors.forename: C E
authors.initials: CE
authors.lastname: Martinez
authors.forename: J C
authors.initials: JC
authors.lastname: Vinay
authors.forename: R
authors.initials: R
authors.lastname: Brieva
authors.forename: C G
authors.initials: CG
authors.lastname: Hill
authors.forename: H S
authors.initials: HS
authors.lastname: Garcia
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000096:biosynthesis*; D004800:Enzymes, Immobilized; D005524:Food Technology / Q000379:methods; D005989:Glycerides / Q000096:biosynthesis* / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism*; D006586:Hexanes; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D020103:Rhizomucor / Q000201:enzymology; D013696:Temperature
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D005989:Glycerides; D006586:Hexanes; D044243:Linoleic Acids, Conjugated; D008049:Lipase; D005990:Glycerol