title: Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I.
abstract: We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtgammaCAL1 and AtgammaCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtgammaCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtgammaCA and AtgammaCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtgammaCAs. All plant species analyzed contain both AtgammaCA and AtgammaCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtgammaCAL sequences show a deviation of functionally important active site residues with respect to gammaCAs but could form active interfaces in the interaction with AtgammaCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.
authors.affiliation: Instituto de Investigaciones Biotecnológicas, IIB-INTECH (CONICET/UNSAM), C.C. 164, 7130 Chascomús, Argentina.
authors.forename: Mariano
authors.initials: M
authors.lastname: Perales
authors.forename: Gustavo
authors.initials: G
authors.lastname: Parisi
authors.forename: María Silvina
authors.initials: MS
authors.lastname: Fornasari
authors.forename: Alejandro
authors.initials: A
authors.lastname: Colaneri
authors.forename: Fernando
authors.initials: F
authors.lastname: Villarreal
authors.forename: Nahuel
authors.initials: N
authors.lastname: González-Schain
authors.forename: Julián
authors.initials: J
authors.lastname: Echave
authors.forename: Diego
authors.initials: D
authors.lastname: Gómez-Casati
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Braun
authors.forename: Alejandro
authors.initials: A
authors.lastname: Araya
authors.forename: Eduardo
authors.initials: E
authors.lastname: Zabaleta
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D019281:Dimerization; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009842:Oligopeptides / Q000235:genetics; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D007527:Isoenzymes; D024101:Mitochondrial Proteins; D009842:Oligopeptides; D002256:Carbonic Anhydrases; D042967:Electron Transport Complex I