On-line monitoring of bioreductions via membrane introduction mass spectrometry.
Real-time and on-line continuous monitoring of reactants, intermediates, and final products for dicarbonyl compound bioreduction in a continuous plug flow reactor packed with baker's yeast (Saccharomyces cerevisiae) whole cells immobilized on calcium alginate beads was performed by membrane introduction mass spectrometry (MIMS) via selective ion monitoring.
Milagre
Cíntia D F
CD
LABIOSIN, Instituto de Química, Universidade Estadual de Campinas CP 6154, CEP 13084-971 Campinas-SP, Brasil.
Milagre
Humberto M S
HM
Rodrigues
J Augusto R
JA
Rocha
Lilian L
LL
Santos
Leonardo S
LS
Eberlin
Marcos N
MN
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods*; D003199:Computer Systems; D017022:Flow Injection Analysis / Q000379:methods; D007659:Ketones / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D008567:Membranes, Artificial; D009862:Online Systems; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D007659:Ketones; D008567:Membranes, Artificial; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase