Relationship between reactive oxygen species production and lipid peroxidation in human sperm suspensions and their association with sperm function.
OBJECTIVE
To investigate if reactive oxygen species (ROS) production by human sperm suspensions could predict oxidative stress manifest as lipid peroxidation and if lipid peroxidation was a useful predictor of human sperm function.


METHODS
Laboratory study.


METHODS
Research laboratory associated with reproductive medicine clinic.


METHODS
Eighty-three couples under evaluation for IVF donated the surplus from a diagnostic semen sample.


METHODS
Cells were separated from seminal plasma on a 30% Percoll gradient.


METHODS
Reactive oxygen species production, lipid peroxidation, sperm motility measured by computer-assisted semen analysis and ability of the sperm to acrosome react.


RESULTS
Forty-four samples exhibited ROS production < or =20 000 relative light units (rlu)/s/10(8) sperm and lipid peroxidation < or =40 nmol malondialdehyde/10(8) sperm. The remainder fell into two distinct groups; both exhibited a linear relationship between lipid peroxidation and ROS production but of markedly differing slopes. Abstinence time had no effect. Lipid peroxidation was a good predictor of sperm motility and their ability to acrosome react.


CONCLUSIONS
Reactive oxygen species production in sperm suspensions is an index of overall oxidative stress experienced by sperm, but sperm divide into two classes of ability to resist oxidative stress. Lipid peroxidation should be investigated as a clinical test of sperm function.
Williams
Andrew Colin
AC
Division of Obstetrics and Gynaecology, University Department of Clinical Science South Bristol, St. Michael's Hospital, Southwell Street, Bristol BS2 8EG, United Kingdom.
Ford
William Christopher Liberty
WC
D020101:Acrosome Reaction / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
D017382:Reactive Oxygen Species