Preparation of sialylated oligosaccharides employing recombinant trans-sialidase from Trypanosoma cruzi.
Terminally sialylated oligosaccharides were synthesised employing recombinant trans-sialidase from Trypanosoma cruzi. Regio- and stereoselectively Sia-alpha(2-3)-Gal-betaR derivatives could be obtained in respectable yields, using combined chemical and enzymatic methodologies. An array of different disaccharide precursors such as Gal-beta(1-3)-GalNAc-alphaSer/Thr, lactosides and lactosamide derivatives were sialylated and successfully purified by facile isolation procedures. Depending on the acceptor structure isolated, yields for trans-sialylation products were between 20 and 60%.
Neubacher
Bjorn
B
Institute of Organic Chemistry, University of Hamburg, Germany.
Schmidt
Dirk
D
Ziegelmuller
Patrick
P
Thiem
Joachim
J
D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D008297:Male; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry*; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology; D014349:Trypanosoma cruzi / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism
D006023:Glycoproteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D012794:Sialic Acids; C069715:trans-sialidase; D009439:Neuraminidase; D001616:beta-Galactosidase