title: Recognition of human cytomegalovirus by human primary immunoglobulins identifies an innate foundation to an adaptive immune response.
abstract: Most primates, including humans, are chronically infected with cospecifically evolved, potentially pathogenic CMV. Abs that bind a 10-aa linear epitope (antigenic determinant 2 site 1) within the extracellular domain of human CMV glycoprotein B neutralize viral infectivity. In this study, we show that genes generated by recombinations involving two well-conserved human germline V elements (IGHV3-30 and IGKV3-11), and IGHJ4, encode primary Ig molecules that bind glycoprotein B at this key epitope. These particular V(H), J(H), and V(kappa) genes enable humans to generate through recombination and N nucleotide addition, a useful frequency of primary Igs that efficiently target this critical site on human CMV and thus confer an innate foundation for a specific adaptive response to this pathogen.
authors.affiliation: The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Gary R
authors.initials: GR
authors.lastname: McLean
authors.forename: Ole A
authors.initials: OA
authors.lastname: Olsen
authors.forename: Ian N
authors.initials: IN
authors.lastname: Watt
authors.forename: P
authors.initials: P
authors.lastname: Rathanaswami
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Leslie
authors.forename: John S
authors.initials: JS
authors.lastname: Babcook
authors.forename: John W
authors.initials: JW
authors.lastname: Schrader
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D003587:Cytomegalovirus / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007113:Immunity, Innate; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D003062:Codon; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus