title: Confinement and crowding effects on tryptophan synthase alpha2beta2 complex.
abstract: Biological molecules experience in vivo a highly crowded environment. The investigation of the functional properties of the tryptophan synthase alpha(2)beta(2) complex either entrapped in wet nanoporous silica gels or in the presence of the crowding agents dextran 70 and ficoll 70 indicates that the rates of the conformational transitions associated to catalysis and regulation are reduced, and an open and less catalytically active conformation is stabilized.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Parma, 43100 Parma, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pioselli
authors.forename: Stefano
authors.initials: S
authors.lastname: Bettati
authors.forename: Andrea
authors.initials: A
authors.lastname: Mozzarelli
mesh_terms: D002384:Catalysis; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D014367:Tryptophan Synthase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014367:Tryptophan Synthase