Oxidative stress and nitric oxide in rats with alcohol-induced acute pancreatitis.
OBJECTIVE
Oxygen free radical mediated tissue damage is well established in pathogenesis of acute pancreatitis (AP). Whether nitric oxide (NO) plays a deleterious or a protective role is unknown. In alcohol-induced AP, we studied NO, lipooxidative damage and glutathione in pancreas, lung and circulation.


METHODS
AP was induced in rats (n = 25) by injection of ethyl alcohol into the common biliary duct. A sham laparatomy was performed in controls (n = 15). After 24 h the animals were killed, blood and tissue sampling were done.


RESULTS
Histopathologic evidence confirmed the development of AP. Marked changes were observed in the pulmonary tissue. Compared with controls, the AP group displayed higher values for NO metabolites in pancreas and lungs, and thiobarbituric acid reactive substances in circulation. Glutathione was lower in pancreas and in circulation. Glutathione and NO were positively correlated in pancreas and lungs of controls but negatively correlated in circulation of experimental group. In the experimental group, plasma thiobarbituric acid reactive substances were negatively correlated with pancreas thiobarbituric acid reactive substances but positively correlated with pancreas NO.


CONCLUSIONS
NO increases in both pancreas and lungs in AP and NO contributes to the pathogenesis of AP under oxidative stress.
Andican
Gülnur
G
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. andican@e-kolay.net
Gelisgen
Remisa
R
Unal
Ethem
E
Tortum
Osman-Baran
OB
Dervisoglu
Sergülen
S
Karahasanoglu
Tayfun
T
Burçak
Gülden
G
D000208:Acute Disease; D000437:Alcoholism / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D005978:Glutathione / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010195:Pancreatitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D005978:Glutathione