Study of Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile for odorant biosensors.
To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we have investigated mixed Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile. Under optimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration of 4 mg L(-1), target pressure 35 mN m(-1)), the mixed monolayer at the air/water interface is very stable and has been efficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers (SAMs) with 1-octadecanethiol (ODT). Atomic force microscopy and electrochemical impedance spectroscopy were used to characterize mixed Langmuir-Blodgett (LB) films before and after contact with a specific odorant molecule, isoamyl acetate. AFM phase images show a higher contrast after contact with the odorant molecule due to the new structure of the OBP-1F/ODA LB film. Non-Faradaic electrochemical spectroscopy (EIS) is used to quantify the effect of the odorant based on the electrical properties of the OBP-1F/ODA LB film, as its resistance strongly decreases from 1.18 MOmega (before contact) to 25 kOmega (after contact).
Hou
Yanxia
Y
Centre de Génie Electrique de Lyon (CEGELY), Ecole Centrale de Lyon, 69134 ECULLY Cedex, France. yanxiahou24@yahoo.com
Jaffrezic-Renault
Nicole
N
Martelet
Claude
C
Tlili
Chaker
C
Zhang
Aidong
A
Pernollet
Jean-Claude
JC
Briand
Loïc
L
Gomila
Gabriel
G
Errachid
Abdelhamid
A
Samitier
Josep
J
Salvagnac
Ludovic
L
Torbiéro
Benoit
B
Temple-Boyer
Pierre
P
D000327:Adsorption; D040761:Biomimetic Materials / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry; D000439:Pentanols / Q000737:chemistry; D018035:Receptors, Odorant / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
D000439:Pentanols; D018035:Receptors, Odorant; D013438:Sulfhydryl Compounds; C045648:odorant-binding protein; C055360:n-octadecyl mercaptan; D006046:Gold; C020377:isoamyl acetate