[The protective effect of recombinant adenovirus of catalase on oxidative damaged rat lens].
OBJECTIVE
To investigate the validity of catalase recombinant adenovirus on the treatment of oxidative cataract.


METHODS
The coding sequence of catalase was cloned and the catalase recombinant adenovirus was constructed. The expression time course of catalase gene in rat lens infected by recombinant adenovirus was determined by Western blotting. Cultured rat lens were randomly divided into 3 groups: the control group, the group treated by hydrogen peroxide and the group treated by hydrogen peroxide combined with catalase recombinant adenovirus. The transparence and apoptosis ratio of lens on the time points of 6, 12, 18, 24 hours were determined by image analysis and double colour flowcytometry.


RESULTS
The coding sequence of catalase was cloned and recombinant adenovirus was successfully constructed. The expression of catalase in cultured rat lens infected by recombinant adenovirus reached peak point on 9 hours post infection and maintained the level in the whole experiment period. The transparence of the lens in the group treated by hydrogen peroxide combined with catalase recombinant adenovirus was higher than that of group treated by hydrogen peroxide and lower than that of the control group on the time points of 6, 12, 18, 24 hours post infection. The differences among groups were statistically significant (P < 0.05). On the same time points, the apoptosis ratio of the group treated by hydrogen peroxide combined with catalase recombinant adenovirus was lower than that of the group treated by hydrogen peroxide and higher than the control group. The differences among groups were statistically significant (P < 0.05).


CONCLUSIONS
The catalase recombinant adenovirus, which can inhibit the turbidity and cell apoptosis of lens caused by oxidant, may be used as the gene therapy of oxidative cataract.
Li
Li-mei
LM
Shantou University/Chinese University of Hongkong Joint International Eye Center, Shantou 515041, China. llm_dn@sina.com
Yang
Xiao-tian
XT
Liu
Ge-fei
GF
Yang
Fu-hui
FH
Zhang
Jin-song
JS
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002374:Catalase / Q000235:genetics*; D005260:Female; D015316:Genetic Therapy; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000633:toxicity; D011995:Recombination, Genetic
D017382:Reactive Oxygen Species; D002374:Catalase