title: Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy.
abstract: Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au(3+)). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable. TOF-SIMS imaging shows the ability to localize directly on a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.
authors.affiliation: Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, France.
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.forename: Frédéric
authors.initials: F
authors.lastname: Halgand
authors.forename: Sabine
authors.initials: S
authors.lastname: De La Porte
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry*; D020388:Muscular Dystrophy, Duchenne / Q000503:physiopathology*; D010712:Phosphatidic Acids / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D012038:Regeneration; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D005989:Glycerides; D008055:Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D006046:Gold