The building blocks for basolateral vesicles in polarized epithelial cells.
After the discovery of basolateral sorting signals for polarized delivery in epithelial cells in the early 1990s, it was only about a decade later that the epithelial-cell-specific sorting adaptor AP-1B was discovered. AP-1B decodes a subclass of basolateral sorting signals and localizes to the recycling endosomes as opposed to the trans-Golgi network, suggesting that this is its major site of action. Furthermore, AP-1B does not simply select its cargo but also facilitates the recruitment of the exocyst complex needed for subsequent fusion with the plasma membrane. This review discusses our current knowledge of AP-1B function in cargo sorting to the basolateral membrane and its impact on our understanding of the similarities and differences between AP-1B-minus fibroblasts and AP-1B-positive epithelial cells.
Fölsch
Heike
H
Dept of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208-3500, USA. h-folsch@northwestern.edu
D033961:Adaptor Protein Complex 1 / Q000502:physiology*; D034001:Adaptor Protein Complex Subunits / Q000737:chemistry; D016764:Cell Polarity; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000737:chemistry; D020558:GTP Phosphohydrolases / Q000378:metabolism; D021382:Protein Sorting Signals; D022161:Transport Vesicles / Q000737:chemistry / Q000502:physiology*
D033961:Adaptor Protein Complex 1; D034001:Adaptor Protein Complex Subunits; D021382:Protein Sorting Signals; D020558:GTP Phosphohydrolases