Morphological and molecular typing of the below-ground fungal community in a natural Tuber magnatum truffle-ground.
The aims of the work were to elucidate the distribution of the ectomycorrhizal fungus Tuber magnatum Pico during its symbiotic stage, and to identify the root-associated fungi in a natural truffle-ground located in North Italy. Ectomycorrhizal root tips were harvested in the truffle ground, sorted in morphotypes and analyzed by ITS. Morphological and molecular analyses revealed that (i) T. magnatum mycorrhizae were rare and independent on the fruitbody productions and (ii) the dominant fungal species belonged to Thelephoraceae, followed by Tuberaceae and Sebacinaceae.
Murat
Claude
C
UMR INRA/UHP 1136 "Interactions Arbres/Micro-Organismes", Centre INRA de Nancy, F-54280 Champenoux, France.
Vizzini
Alfredo
A
Bonfante
Paola
P
Mello
Antonietta
A
D001203:Ascomycota / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer