Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh.
In previous studies with strains of the Shigella dysenteriae provisional serovars E22383 and E23507 from diarrhoeal stools from patients in Bangladesh, two strains of Shigella species were identified as Shigella boydii provisional serovar E16553 by a reference laboratory. Further tests with an antiserum to an international type strain of the provisional serovar E16553 identified an additional 15 isolates. None of the isolates reacted with antisera to the established Shigella serovars or any other provisional serovars reported so far and all showed biochemical reactions typical of S. boydii. All of the isolates harboured the 140 MDa invasion plasmid, had the ipaH gene and produced keratoconjunctivitis in the guinea pig eye. All isolates were susceptible to ampicillin, sulfamethoxazole-trimethoprim, nalidixic acid, ciprofloxacin and mecillinam but eight strains were resistant to tetracycline. A single PFGE type (type A) was shown for all 17 clinical isolates, indicating a common source of origin. The pulsotype of the Bangladeshi isolates was closely related to that of a Japanese strain but was different from that of the type strain. On the basis of these biochemical, serological and virulence markers, and diverse geographical origin, it is recommended that the provisional status of serovar E16553 be changed and that it be included in the international serotyping classification scheme as S. boydii 19.
Ansaruzzaman
M
M
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Sultana
Marzia
M
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Talukder
Kaisar A
KA
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Alam
K
K
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Matsushita
S
S
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Safa
Ashrafus
A
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Khajanchi
Bijay K
BK
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Dutta
Dilip K
DK
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Islam
Zhahirul
Z
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Albert
M John
MJ
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Nair
G Balakrish
GB
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Sack
David A
DA
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh 2Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan 3Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001459:Bangladesh; D004352:Drug Resistance, Microbial; D004405:Dysentery, Bacillary / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D007637:Keratoconjunctivitis / Q000382:microbiology; D012761:Shigella boydii / Q000145:classification* / Q000472:pathogenicity / Q000502:physiology; D012762:Shigella dysenteriae / Q000145:classification; D014774:Virulence
D000890:Anti-Infective Agents