Expression of 8-oxoguanine DNA glycosylase (OGG1) in Parkinson's disease and related neurodegenerative disorders.
Oxidative stress including DNA oxidation is implicated in Parkinson's disease (PD). We postulated that DNA repair enzymes such as 8-oxoguanosine DNA glycosylase (OGG1) are involved in the PD process. We performed immunohistochemical and biochemical studies on brains of patients with PD and those of patients with progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD) as disease controls, and control subjects. We found higher expression levels of mitochondrial isoforms of OGG1 enzymes in the substantia nigra (SN) in cases of PD. Furthermore, Western blot analysis revealed high OGG1 levels in the SN of the patients with PD. Our results indicate the importance of oxidative stress within the susceptible lesions in the pathogenesis of PD.
Fukae
Jiro
J
Department of Neurology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan.
Takanashi
Masashi
M
Kubo
Shin-ichiro
S
Nishioka
Ken-ichi
K
Nakabeppu
Yusaku
Y
Mori
Hideo
H
Mizuno
Yoshikuni
Y
Hattori
Nobutaka
N
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001480:Basal Ganglia Diseases / Q000201:enzymology*; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D002452:Cell Count / Q000379:methods; D045647:DNA Glycosylases / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D010300:Parkinson Disease / Q000201:enzymology*; D011180:Postmortem Changes; D013347:Subcellular Fractions / Q000201:enzymology; D013494:Supranuclear Palsy, Progressive / Q000201:enzymology*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D003576:Electron Transport Complex IV; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human