title: Mode of action of piscicocin CS526 produced by Carnobacterium piscicola CS526.
abstract: OBJECTIVE
  Piscicocin CS526 is a unique class IIa bacteriocin produced by Carnobacterium piscicola CS526. The mode of action against the sensitive strain Listeria monocytogenes IID581 was evaluated.
  
  
  RESULTS
  Piscicocin CS526 was adsorbed on both sensitive and insensitive gram-positive and gram-negative bacterial cells. Treatment of L. monocytogenes cells with trypsin, lipase and Triton X-100 did not reduce subsequent adsorption of piscicocin CS526. The activity of piscicocin CS526 against L. monocytogenes cells was bactericidal rather than bacteriostatic, but did not cause bacteriolysis. Piscicocin CS526 induced the efflux of K+ ions from the target cells which cause dissipation of the transmembrane potential (DeltaPsi) of the cell membrane. Moreover, after exposure to piscicocin CS526, intracellular adenosine 5'-triphosphate (ATP) level of the target cells rapidly reduced without leakage of ATP from the cells, indicating that ATP depletion occurred in the cells.
  
  
  CONCLUSIONS
  Pore formation by piscicocin CS526 caused a rapid efflux of small molecules such as K+ from the indicator cells and dissipation of proton motive force (PMF), which lead to the cell death.
  
  
  CONCLUSIONS
  Molecular mechanism of action of piscicocin CS526 is very similar to that of other pediocin-like bacteriocins, although piscicocin CS526 possesses a unique N-terminal sequence in which Val is substituted for by Leu in the amino acid at position 7.
authors.affiliation: Laboratory of Marine Food Science, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kawai
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D001431:Bacteriological Techniques; D003902:Detergents / Q000494:pharmacology; D005399:Fishes; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D017830:Octoxynol / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003902:Detergents; D000255:Adenosine Triphosphate; D017830:Octoxynol; D008049:Lipase; D014357:Trypsin; D011188:Potassium