In vitro bioactivity of MOEP grafted ePTFE membranes for craniofacial applications.
The bioactivity of three methacryloyloxyethyl phosphate (MOEP) grafted expanded polytetrafluoroethylene (ePTFE) membranes with varying surface coverage as well as unmodified ePTFE was investigated through a series of in vitro tests: calcium phosphate (CaP) growth in simulated body fluid (SBF), serum protein adsorption, and a morphology and attachment study of human osteoblast-like SaOS-2 cells. The graft copolymers were prepared by means of gamma irradiation induced grafting and displayed various surface morphologies and wettabilities depending on the grafting conditions used. Unmodified ePTFE did not induce nucleation of CaP minerals, whereas all the grafted membranes revealed the growth of CaP minerals after 7 days immersion in SBF. The sample with lowest surface grafting yield (24% coverage), a smooth graft morphology and relatively high hydrophobicity (theta(adv) = 120 degrees, theta(rec) = 80 degrees) showed carbonated hydroxyapatite growth covering the surface. On the other hand, the samples with high surface grafting yield (76% and 100%), a globular graft morphology and hydrophilic surfaces (theta(adv) = 60 degrees and 80 degrees, theta(rec) = 25 degrees and 15 degrees, respectively) exhibited irregular growth of non-apatitic CaP minerals. Irreversibly adsorbed protein measured after a 1h immersion in serum solution was quantified by the amount of nitrogen on the surface using XPS, as well as by weight increase. All grafted membranes adsorbed 3-6 times more protein than the unmodified membrane. The sample with the highest surface coverage adsorbed the most protein. Osteoblast-like SaOS-2 cells cultured for 3 h revealed significantly higher levels of cell attachment on all grafted membranes compared to unmodified ePTFE. Although the morphology of the cells was heterogeneous, in general, the higher grafted surfaces showed a much better cell morphology than both the low surface-grafted and the control unmodified sample. The suite of in vitro tests confirms that a judicious choice of grafted monomer such as the phosphate-containing methacrylate monomer (MOEP) significantly improves the bioactivity of ePTFE in vitro.
Suzuki
Shuko
S
Tissue BioRegeneration and Integration Program, School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane QLD, Australia.
Grøndahl
Lisbeth
L
Leavesley
David
D
Wentrup-Byrne
Edeline
E
D000327:Adsorption; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D019465:Craniofacial Abnormalities / Q000601:surgery; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008689:Methacrylates / Q000032:analysis / Q000737:chemistry*; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology* / Q000637:transplantation; D011138:Polytetrafluoroethylene / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D019651:Plastic Surgery Procedures / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; D008689:Methacrylates; C026575:2-hydroxyethyl methacrylate phosphate; D011138:Polytetrafluoroethylene