title: DNA hypermethylation of tumor-related genes in gastric carcinoma.
abstract: The hypermethylation of the CpG islands is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed the methylation status of genes for cell repair such as hMLH1, MGMT, and GSTP1, and a gastric cancer-specifically methylated DNA fragment, MINT 25 in gastric cancer cases and control groups. The study population consisted of 100 gastric cancer patients (50 distal and 50 proximal carcinomas), and 238 healthy controls. All genes showed more frequent hypermethylation in the cases than in the control group (p<0.0001). We investigated the association between promoter hypermethylation and relevant parameters including age, gender, alcohol consumption, smoking, and family history. There was a common hypermethylation of hMLH1 (p=0.008), MGMT (p= 0.0001), and GSTP1 (p=0.0003) in females. This study also demonstrates that hypermethylation was strongly associated with non-drinkers (MGMT, p=0.046 and MINT 25, p=0.049) and non-smokers (hMLH1, p=0.044; MGMT, p=0.0003; MINT 25, p=0.029). Moreover, the frequency of MINT 25 hypermethylation increased with age (p=0.037), and MGMT methylation was frequently detected in distal gastric cancer than in proximal type (p=0.038). Our study suggested that promoter hypermethylation of the genes involved in cell repair system and MINT 25 is associated strongly with some subgroups of primary gastric carcinoma.
authors.affiliation: Department of Dental Microbiology, Kyungpook National University School of Dentistry, Daegu 700-422, Korea.
authors.forename: Su Hyung
authors.initials: SH
authors.lastname: Hong
authors.forename: Ho Gak
authors.initials: HG
authors.lastname: Kim
authors.forename: Woon Bok
authors.initials: WB
authors.lastname: Chung
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Jong Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Sang Mo
authors.initials: SM
authors.lastname: Yoon
authors.forename: Joong Goo
authors.initials: JG
authors.lastname: Kwon
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Sohn
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Kwak
authors.forename: Jung Wan
authors.initials: JW
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D002352:Carrier Proteins; D019175:DNA Methylation; D005260:Female; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D007527:Isoenzymes; C501486:MLH1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D019853:O(6)-Methylguanine-DNA Methyltransferase; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D000070957:MutL Protein Homolog 1