title: Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica.
abstract: Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.
authors.affiliation: Department of Parasitology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Okada
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Huston
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Mann
authors.forename: William A
authors.initials: WA
authors.lastname: Petri
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kita
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Nozaki
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004748:Entamoeba histolytica / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D010587:Phagocytosis; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism / Q000502:physiology*; D000090802:rab7 GTP-Binding Proteins
chemical_list: D000199:Actins; D015415:Biomarkers; D015800:Protozoan Proteins; D000090802:rab7 GTP-Binding Proteins; D020558:GTP Phosphohydrolases; C464925:Rab7 protein, Entamoeba histolytica