Monitoring dioxins in food and feedstuffs using accelerated solvent extraction with a novel integrated carbon fractionation cell in combination with a CAFLUX bioassay.
The concentrations of dioxins in fish oil and fish meal were determined with accelerated solvent extraction, using a novel integrated carbon fractionation extraction cell followed by a miniturized multilayer silica column and bioanalysis on a recently-developed chemically-activated fluorescent gene expression cell bioassay. The developed method allows for simultaneous gravimetric lipid weight determination, which was shown for both matrices under study (about 100% lipid recovery of each sample). Initial results practically meet the quality criteria on screening methods for control of dioxins in food and feedstuffs laid down in the EU Commission Directives 2002/69/EC (food) and 2002/70/EC (feed). This demonstrates that the developed method can be used as a screening tool for monitoring dioxins in food and feed after some additional improvements and testing on a greater number of matrices.
Nording
Malin
M
Environmental Chemistry, Department of Chemistry, Umeå University, 901 87 Umeå, Sweden. malin.nording@chem.umu.se
Sporring
Sune
S
Wiberg
Karin
K
Björklund
Erland
E
Haglund
Peter
P
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004147:Dioxins / Q000032:analysis*; D005395:Fish Oils; D005396:Fish Products; D005506:Food Contamination / Q000032:analysis*; D049452:Green Fluorescent Proteins; D012997:Solvents
D004147:Dioxins; D005395:Fish Oils; D012997:Solvents; D049452:Green Fluorescent Proteins