Functional interaction of Oct transcription factors with the family of repeats in Epstein-Barr virus oriP.
The family of repeats (FR) is a major upstream enhancer of the Epstein-Barr virus (EBV) latent C promoter (Cp) that controls transcription of six different latent nuclear proteins following interaction with the EBV nuclear protein EBNA1. Here, it was shown that Cp could also be activated by octamer-binding factor (Oct) proteins. Physical binding to the FR by the cellular transcription factors Oct-1 and Oct-2 was demonstrated by using an electrophoretic mobility-shift assay. Furthermore, Oct-1 in combination with co-regulator Bob.1, or Oct-2 alone, could drive transcription of a heterologous thymidine kinase promoter linked to the FR in both B cells and epithelial cells. Cp controlled by the FR was also activated by binding of Oct-2 to the FR. This may have direct implications for B cell-specific regulation of Cp.
Almqvist
J
J
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Nobels väg 16, Box 280, S-171 77 Stockholm, Sweden.
Zou
J
J
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Nobels väg 16, Box 280, S-171 77 Stockholm, Sweden.
Linderson
Y
Y
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Nobels väg 16, Box 280, S-171 77 Stockholm, Sweden.
Borestrom
C
C
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden.
Altiok
E
E
Acibadem Genetic Diagnostic Center, Libadiye Cad, Bogazici Sitesi, Goztepe, 34724 Istanbul, Turkey.
Zetterberg
H
H
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden.
Rymo
L
L
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden.
Pettersson
S
S
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Nobels väg 16, Box 280, S-171 77 Stockholm, Sweden.
Ernberg
I
I
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Nobels väg 16, Box 280, S-171 77 Stockholm, Sweden.
D001402:B-Lymphocytes / Q000821:virology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000821:virology; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*; D018741:Replication Origin / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
D004268:DNA-Binding Proteins; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; D014157:Transcription Factors