Inhibition of influenza A virus replication by resveratrol.
We have previously shown that the life cycles of several viruses are influenced by host-cell redox states. Reports of the antioxidant activities of the plant polyphenol resveratrol (RV) prompted us to investigate its effects on influenza virus replication in vitro and in vivo. We found that RV strongly inhibited the replication of influenza virus in MDCK cells but that this activity was not directly related to glutathione-mediated antioxidant activity. Rather, it involved the blockade of the nuclear-cytoplasmic translocation of viral ribonucleoproteins and reduced expression of late viral proteins seemingly related to the inhibition of protein kinase C activity and its dependent pathways. RV also significantly improved survival and decreased pulmonary viral titers in influenza virus-infected mice. No toxic effects were observed in vitro or in vivo. That RV acts by inhibiting a cellular, rather than a viral, function suggests that it could be a particularly valuable anti-influenza drug.
Palamara
Anna T
AT
Institute of Microbiology, University of Rome La Sapienza, Rome, Italy. annateresa.palamara@uniroma1.it
Nencioni
Lucia
L
Aquilano
Katia
K
De Chiara
Giovanna
G
Hernandez
Leyanis
L
Cozzolino
Federico
F
Ciriolo
Maria R
MR
Garaci
Enrico
E
D000818:Animals; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000821:virology; D010084:Oxidation-Reduction; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects*
D004791:Enzyme Inhibitors; D013267:Stilbenes; D014764:Viral Proteins; D000077185:Resveratrol