title: Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies.
abstract: A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis.
authors.affiliation: Instituto de Biologíia Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia, Laboratorio de Genómica, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Forment
authors.forename: J
authors.initials: J
authors.lastname: Gadea
authors.forename: L
authors.initials: L
authors.lastname: Huerta
authors.forename: L
authors.initials: L
authors.lastname: Abizanda
authors.forename: J
authors.initials: J
authors.lastname: Agusti
authors.forename: S
authors.initials: S
authors.lastname: Alamar
authors.forename: E
authors.initials: E
authors.lastname: Alos
authors.forename: F
authors.initials: F
authors.lastname: Andres
authors.forename: R
authors.initials: R
authors.lastname: Arribas
authors.forename: J P
authors.initials: JP
authors.lastname: Beltran
authors.forename: A
authors.initials: A
authors.lastname: Berbel
authors.forename: M A
authors.initials: MA
authors.lastname: Blazquez
authors.forename: J
authors.initials: J
authors.lastname: Brumos
authors.forename: L A
authors.initials: LA
authors.lastname: Canas
authors.forename: M
authors.initials: M
authors.lastname: Cercos
authors.forename: J M
authors.initials: JM
authors.lastname: Colmenero-Flores
authors.forename: A
authors.initials: A
authors.lastname: Conesa
authors.forename: B
authors.initials: B
authors.lastname: Estables
authors.forename: M
authors.initials: M
authors.lastname: Gandia
authors.forename: J L
authors.initials: JL
authors.lastname: Garcia-Martinez
authors.forename: J
authors.initials: J
authors.lastname: Gimeno
authors.forename: A
authors.initials: A
authors.lastname: Gisbert
authors.forename: G
authors.initials: G
authors.lastname: Gomez
authors.forename: L
authors.initials: L
authors.lastname: Gonzalez-Candelas
authors.forename: A
authors.initials: A
authors.lastname: Granell
authors.forename: J
authors.initials: J
authors.lastname: Guerri
authors.forename: M T
authors.initials: MT
authors.lastname: Lafuente
authors.forename: F
authors.initials: F
authors.lastname: Madueno
authors.forename: J F
authors.initials: JF
authors.lastname: Marcos
authors.forename: M C
authors.initials: MC
authors.lastname: Marques
authors.forename: F
authors.initials: F
authors.lastname: Martinez
authors.forename: M A
authors.initials: MA
authors.lastname: Martinez-Godoy
authors.forename: S
authors.initials: S
authors.lastname: Miralles
authors.forename: P
authors.initials: P
authors.lastname: Moreno
authors.forename: L
authors.initials: L
authors.lastname: Navarro
authors.forename: V
authors.initials: V
authors.lastname: Pallas
authors.forename: M A
authors.initials: MA
authors.lastname: Perez-Amador
authors.forename: J
authors.initials: J
authors.lastname: Perez-Valle
authors.forename: C
authors.initials: C
authors.lastname: Pons
authors.forename: I
authors.initials: I
authors.lastname: Rodrigo
authors.forename: P L
authors.initials: PL
authors.lastname: Rodriguez
authors.forename: C
authors.initials: C
authors.lastname: Royo
authors.forename: R
authors.initials: R
authors.lastname: Serrano
authors.forename: G
authors.initials: G
authors.lastname: Soler
authors.forename: F
authors.initials: F
authors.lastname: Tadeo
authors.forename: M
authors.initials: M
authors.lastname: Talon
authors.forename: J
authors.initials: J
authors.lastname: Terol
authors.forename: M
authors.initials: M
authors.lastname: Trenor
authors.forename: L
authors.initials: L
authors.lastname: Vaello
authors.forename: O
authors.initials: O
authors.lastname: Vicente
authors.forename: Ch
authors.initials: Ch
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: Zacarias
authors.forename: V
authors.initials: V
authors.lastname: Conejero
mesh_terms: D002957:Citrus / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D018749:RNA, Plant