Separation of hyaluronic acid by ultrahigh-voltage capillary gel electrophoresis.
Hyaluronic acid was separated using 95 kV applied potential in a polyacrylamide gel-filled capillary. The results of this separation were compared to those obtained using a capillary electrophoresis instrument operated at a more conventional potential of 15 kV. For lower-molecular-weight oligomers, the separation efficiency was found to improve by about tenfold, and the resolution by about threefold. However, the improvement in resolution declined as the polymer molecular weight increased.
Hutterer
Katariina M
KM
Department of Chemistry, University of North Carolina at Chapel Hill, NC, USA.
Jorgenson
James W
JW
D019075:Electrophoresis, Capillary / Q000379:methods*; D006820:Hyaluronic Acid / Q000302:isolation & purification*; D007834:Lasers; D008970:Molecular Weight; D013050:Spectrometry, Fluorescence
D006820:Hyaluronic Acid