[Platelet-rich plasma made by a modified method promotes proliferation of rat osteoblast and human osteoblast in vitro].
OBJECTIVE
To study the effect of serum rich in growth factors (SRGF) derived from platelet-rich plasma (PRP) on the biological function of human and rat osteoblast.


METHODS
PRP and platelet-poor plasma (PPP) obtained from healthy human and SD rat were activated by thrombin to get SRGF and serum poor in growth factors (SPGF). The level of TGF-beta1 and PDGF-AB in human-SRGF and SPGF were assayed by enzyme-linked immunoassay (ELISA). Rat and human osteoblast were cultured and identified. Rat osteoblasts were treated with 5% rat-SRGF, 5% rat-SPGF and serum-free F12 medium, respectively. And human osteoblast were treated with 5% human-SRGF, 5% human-SPGF and serum-free DMEM. Cellular mitogenic activity was evaluated by thiazolyl blue (MTT) colorimetric assay at 24, 48, 72 and 96 hours.


RESULTS
The level of TGF-beta1 in human-SRGF was 307.67 +/- 35.57 ng/ml, and that of PDGF-AB was 52.76 +/- 7.89 ng/ml. The proliferation of rat and human osteoblast were promoted after treated with rat-SRGF and human-SRGF, respectively. In rat osteoblast groups, there were significant differences in absorbency between rat-SPGF group and rat-SRGF group at 48 and 96 hours (P < 0.05). In human osteoblast groups, the differences between human-SPGF group and human-SRGF group were significant at 48, 72 and 96 hours (P < 0.05). The proliferation of these two kinds of osteoblasts almost stopped in serum-free medium, and the differences in absorbency, compared with other groups, were significant (P < 0.05).


CONCLUSIONS
High quality of PRP can be achieved by the improved method and SRGF is capable of up-regulating the proliferation of rat osteoblast and human osteoblast.
Song
Yang
Y
Department Prosthodontics, West China College of Stomatology, Sichuan University, Chengdu Sichuan 610041, PR China.
Chao
Yonglie
Y
Gong
Ping
P
D000293:Adolescent; D000328:Adult; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D053773:Transforming Growth Factor beta1 / Q000378:metabolism / Q000494:pharmacology*
D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1