An in vitro study of cytotoxic effects of gossypol on human epidermoid larynx carcinoma cell line (HEp-2).
OBJECTIVE
The study was aimed on an evaluation of the effect of gossypol at concentration of 25-150 microM on the level of apoptosis in the human epidermoid larynx carcinoma cell line, HEp-2, in vitro.


METHODS
To determine the apoptotic effects of gossypol on growth of human epidermoid larynx carcinoma cell lines (HEp-2 cells), morphological evaluation of the cells and DNA fragmentation analysis were used.


RESULTS
6 h incubation of HEp-2 cells with gossypol at concentrations of 50 microM and 75 microM resulted in the increase of apoptosis rate by 26 and 23%, respectively.


CONCLUSIONS
This preliminary study may constitute a base for in vivo studies of anticancer properties of gossypol.
Konac
Ece
E
Department of Medical Biology and Genetics, Gazi University, Faculty of Medicine, Besevler, Ankara, Turkey.
Ekmekci
Abdullah
A
Yurtcu
Erkan
E
Ergun
Mehmet Ali
MA
D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006072:Gossypol / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000473:pathology*
D000970:Antineoplastic Agents; D006072:Gossypol