title: 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose induces apoptosis in Hep G2 cells.
abstract: OBJECTIVE
  To determine the effect of 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose (COPADG) on proliferation and apoptosis of human hepatocellular carcinoma cells (Hep G2).
  
  
  METHODS
  Hep G2 cells were cultured in RPMI-1640 medium in the presence of various concentrations of COPADG. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.
  
  
  RESULTS
  At the concentration ranging between 1-30 microM, COPADG potently inhibits growth and induce apoptosis of Hep G2 cells.
  
  
  CONCLUSIONS
  COPADG could effectively induce apoptosis in Hep G2 cells. It may be potentially useful as a new agent for treatment of human hepatocellular carcinoma.
authors.affiliation: Department of Gastroenterology and Hepatology, the First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Delu
authors.initials: D
authors.lastname: Qi
authors.forename: Kou
authors.initials: K
authors.lastname: Wei
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
authors.forename: Aiqing
authors.initials: A
authors.lastname: Wang
authors.forename: Weiming
authors.initials: W
authors.lastname: Liu
authors.forename: Qunji
authors.initials: Q
authors.lastname: Xue
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D046529:Microscopy, Electron, Transmission
chemical_list: D003847:Deoxyglucose