CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines.
In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion.
Sconocchia
Giuseppe
G
Hematology Branch, Stem Cell Allotransplantation Section, National Hearth Lung and Blood Institute, Bethesda, MD, USA. Barrettj@nhlbi.nih.gov.
Provenzano
Maurizio
M
Rezvani
Katayoun
K
Li
Jongming
J
Melenhorst
Jos
J
Hensel
Nancy
N
Barrett
A John
AJ