Pathological prion protein in the tongues of sheep infected with naturally occurring scrapie.
Tongue involvement by prion spreading was shown to be a common outcome after oral or intracranial experimental challenge with scrapie and transmissible mink encephalopathy sources in rodent models. It is also known that bovine spongiform encephalopathy, which is pathogenic for humans, is experimentally transmissible to sheep and can lead to a disease indistinguishable from scrapie. A recent European Food Safety Authority opinion recommended research into PrPsc accumulation in the tongues of ruminants. We report on the detection of PrPsc in the tongues of seven scrapie-infected sheep by immunohistochemistry and Western blotting.
Casalone
Cristina
C
CEA-IZS Piemonte, Liguria e Valle d'Aosta, Via Bologna, 148 Turin 10154, Italy.
Corona
Cristiano
C
Crescio
Maria Ines
MI
Martucci
Francesca
F
Mazza
Maria
M
Ru
Giuseppe
G
Bozzetta
Elena
E
Acutis
Pier Luigi
PL
Caramelli
Maria
M
D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D018620:PrPSc Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D014059:Tongue / Q000033:anatomy & histology / Q000378:metabolism*
D018620:PrPSc Proteins