title: Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol-gel-glued cyclodextrin-modified silica particles.
abstract: By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kV) and pressure (300 bar) and possesses a high efficiency (up to 100,000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.
authors.affiliation: Institute of Organic Chemistry, University of Tübingen, D-72076 Tübingen, Germany. dorothee.wistuba@uni-tuebingen.de
authors.forename: Dorothee
authors.initials: D
authors.lastname: Wistuba
authors.forename: Leena
authors.initials: L
authors.lastname: Banspach
authors.forename: Volker
authors.initials: V
authors.lastname: Schurig
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D005782:Gels; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D003505:Cyclodextrins; D005782:Gels; D012822:Silicon Dioxide