[NOD2/CARD15 gene polymorphisms and susceptibility to Crohn's disease in Chinese Han population].
OBJECTIVE
Previous studies have shown NOD2/CARD15 gene is the first susceptibility gene to Crohn's disease (CD), three single nucleotide polymorphisms (SNPs) of the gene have been identified to be associated with CD in the Caucasians, but not in the Japanese. Here we have evaluated the NOD2/CARD gene polymorphisms in Chinese patients to determine whether the gene is associated with susceptibility to CD in Chinese Han population.


METHODS
Blood samples were obtained from 32 patients with CD, 110 patients with ulcerative colitis, and 292 healthy controls in Zhejiang location. Genotyping for 3 common NOD2/CARD15 (Arg702Trp, Gly908Arg, Leu1007fsinsC) polymorphisms was carried out using polymerase chain sequence with specific primer.


RESULTS
None of the patients with CD had heterozygous or homozygous SNPs variants. Similarly none of the ulcerative colitis or health controls.


CONCLUSIONS
The common variants in NOD2/CARD15 found in Caucasians with CD are not associated with CD in the Chinese Han population.
Gao
Min
M
GI Division of Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Cao
Qian
Q
Luo
Ling-he
LH
Wu
Min-liang
ML
Hu
Wei-ling
WL
Si
Jian-min
JM
D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000208:ethnology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D053473:Nod2 Signaling Adaptor Protein; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D047908:Intracellular Signaling Peptides and Proteins; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein