Metal ion promoted hydrogels for bovine serum albumin adsorption: Cu(II) and Co(II) chelated poly[(N-vinylimidazole)-maleic acid].
Poly[(N-vinylimidazole)-maleic acid] (poly(VIm-MA)), copolymeric hydrogels were prepared by gamma-irradiating ternary mixtures of N-vinylimidazole-maleic acid-water in a (60)Co-gamma source. Cu(II) and Co(II) ions were chelated within the gels at pH=5.0. The maximum adsorption capacity of the gels were 3.71 mmol/g dry gel for Cu(II) and 1.25 mmol/g dry gel for Co(II) at pH=5.0. The swelling ratios of the gels were 1200% for poly(VIm-MA), 60 and 45% for Cu(II) and Co(II)-chelated poly(VIm-MA) gels at pH=5.0 in acetate buffer solution. These affinity gels with different swelling ratios for plain poly(VIm-MA), Cu(II)-, and Co(II)-chelated poly(VIm-MA), in acetate and phosphate buffers were used in the bovine serum albumin (BSA) adsorption/desorption studies in batch reactor. The maximum BSA adsorption capacities of the gels were 0.38 g/g dry gel for plain, 0.88 g/g dry gel for Cu(II)-chelated poly(VIm-MA) and 1.05 g/g dry gel for Co(II)-chelated poly(VIm-MA) gels. Adsorption capacity of BSA by the gels was reduced dramatically by increasing the ionic strength adjusted with NaCl. More than 95% of BSA were desorbed in 10 h in desorption medium containing 0.1M of EDTA for metal ion-chelated gels at pH=4.7.
Pekel
Nursel
N
Department of Chemistry, Hacettepe University, Beytepe, 06532 Ankara, Turkey.
Salih
Bekir
B
Güven
Olgun
O
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007477:Ions; D007700:Kinetics; D008298:Maleates / Q000737:chemistry*; D008956:Models, Chemical; D012709:Serum Albumin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013861:Thiocyanates / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002614:Chelating Agents; D003102:Colloids; D020100:Hydrogels; D007093:Imidazoles; D007477:Ions; D008298:Maleates; D012709:Serum Albumin; D013861:Thiocyanates; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003035:Cobalt; D003300:Copper; C029899:imidazole; C030272:maleic acid; D004492:Edetic Acid; C009941:potassium thiocyanate