[Determination of protein in urine by spectral probe of molybdenum(VI) salicylfluoronate complex].
The absorption spectral behavior of binding reaction of molybdenum(VI)-salicylfluoronate on bovine serum albumin was investigated. A novel method for the determination of protein in urine using Mo(VI)-salicylfluoronate complex as a spectroprobe was developed. The maximum binding number (n) of molybdenum complex to BSA was found to be 100. The molar absorptivity of binding product at 575 nm was 2.86 x 10(6) L x mol(-1) x cm(-1). The method possesses high sensitivity as well as high selectivity. It could be used to determine protein in human urine directly. The relative standard deviation for the determination is 1.5%.
Shen
H
H
Department of Chemistry, Nankai University, 300071 Tianjin.
Liu
L
L
Liu
Y
Y
D006801:Humans; D008982:Molybdenum / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D014556:Urine / Q000737:chemistry*
D011506:Proteins; D008982:Molybdenum