Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs).
[structure: see text] The alpha-l-threofuranosyl nucleoside triphosphates of T, G, and D (tTTP, tGTP, and tDTP) were synthesized from the described 2'-O-DMT-protected derivatives using the Eckstein method, while the corresponding C derivative (tCTP) was prepared from the 2'-O-acetyl derivative. The prepared alpha-l-threofuranosyl nucleoside triphosphates, despite being one carbon shorter than the native 2'-deoxyfuranosyl nucleoside triphosphates, are effective substrates for selected DNA polymerases.
Zou
Keyong
K
Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02184, USA.
Horhota
Allen
A
Yu
Biao
B
Szostak
Jack W
JW
McLaughlin
Larry W
LW
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013237:Stereoisomerism
D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase