Continuous fabrication of biocatalyst immobilized microparticles using photopolymerization and immiscible liquids in microfluidic systems.
We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and hydrogel solutions form emulsions in pressure-driven flow in microchannels at high values of the dimensionless capillary number (Ca). The resultant hydrogel droplets are then polymerized in situ via exposure to 365 nm UV light. This technique allows for the generation of monodisperse particles whose size can be controlled by the regulation of flow rates. In addition, both manufacturing microparticles and immobilizing biocatalysts can be performed simultaneously and continuously.
Jeong
Won Je
WJ
Kim
Jeong Yun
JY
Choo
Jaebum
J
Lee
Eun Kyu
EK
Han
Chang Soo
CS
Beebe
David J
DJ
Seong
Gi Hun
GH
Lee
Sang Hoon
SH
D015374:Biosensing Techniques; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010316:Particle Size; D010777:Photochemistry
D004800:Enzymes, Immobilized; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase