title: Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant.
abstract: An outbreak of canine parvovirus type 2 infection caused by the Glu-426 mutant in 2 litters of pups is reported. The infected pups (n = 6) were monitored daily for evidence of clinical signs and hematological changes and for the evaluation of viral shedding in the feces. The disease induced by the Glu-426 mutant was mild in all the infected pups. Vomiting and hemorrhagic diarrhea were not observed; however, the pups developed mucoid diarrhea (3.5 median days), depression (1.5 median days), and relative leukopenia and lymphopenia (2.5 median days). Fever and loss of appetite were observed only in 2 pups. Virus was detected in the feces for 4.5, 6.5, and 46 median days by hemagglutination, virus isolation on cell cultures, and real-time polymerase chain reaction (PCR), respectively. By real-time PCR, the highest viral DNA titers were detected in the feces of both litters at day 10, reaching median values of more than 10(10) DNA copies/mg of feces.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Marco
authors.initials: M
authors.lastname: Campolo
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Dominga
authors.initials: D
authors.lastname: Ricci
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lorusso
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000737:chemistry; D004196:Disease Outbreaks / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005260:Female; D006386:Hemagglutination Tests / Q000662:veterinary; D007558:Italy / Q000453:epidemiology; D008297:Male; D009154:Mutation; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral