TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs.
Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an approximately 700-bp integration window upstream of tRNA genes with a periodicity of approximately 80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.
Bachman
Nurjana
N
The Johns Hopkins University School of Medicine, Department of Molecular Biology and Genetics, Baltimore, Maryland 21205, USA.
Gelbart
Marnie E
ME
Tsukiyama
Toshio
T
Boeke
Jef D
JD
D000251:Adenosine Triphosphatases / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013045:Species Specificity; D035361:Transcription Factor TFIIIB / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
C474805:Bdp1 protein, S cerevisiae; D017931:DNA Primers; C086468:ISWI protein; D009707:Nucleosomes; D021122:Protein Subunits; D018626:Retroelements; D029701:Saccharomyces cerevisiae Proteins; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors; D012343:RNA, Transfer; D000251:Adenosine Triphosphatases