Antibody immobilisation on the metal and silicon surfaces. The use of self-assembled layers and specific receptors.
The use of Staphylococcal protein A and lectins as intermediate immobilising agents allows operators to orient antibodies (Ab) towards the solution due to the presence of a specific binding sites of immunoglobulin (Ig) molecules. Antibodies of different species of animals have unequal affinities to individual lectins. The effective thickness of immobilised Ab's depends on the type of substrates used and increases in the following sequence: bare gold or silicon surface, the surface treated with self-assembled polyelectrolytes (PESA) or with protein A or some lectins deposited on the preliminary formed polyelectrolyte layer. The glycolysated protein of jp51 may be selectively immobilised from the mixture of retroviral proteins (p24 and jp51), if it is necessary to distinguish infected animals from preliminarily immunised ones by means of a vaccine based on p24 protein. It was shown that the use of Staphylococcal protein A, instead of some lectins as intermediate layer for the Ab immobilisation, does not lead to a more sensitive determination of such low-weight toxins as 2,4-dichlorophenoxyacetic acid (2,4-D). The above-mentioned results were obtained with surface plasmon resonance (SPR) technique.
Starodub
N F
NF
Department of Biochemistry of Sensory and Regulatory Systems, A.V. Palladin Institute of Biochemistry, Ukrainian National Academy of Sciences, 9 Leontovicha Str., 01030 Kiev 30, Ukraine. nstarodub@hotmail.com
Pirogova
L V
LV
Demchenko
A
A
Nabok
A V
AV
D000906:Antibodies / Q000737:chemistry*; D001666:Binding Sites, Antibody; D006046:Gold / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D000906:Antibodies; D037102:Lectins; D013205:Staphylococcal Protein A; D006046:Gold; D012825:Silicon