title: [Cloning, expression, purification and characterization of human endostatin gene].
abstract: OBJECTIVE
  To procure biologically active human endostatin.
  
  
  METHODS
  Human endostatin gene was acquired by means of reverse transcriptase (RT)-PCR and cloned into PGEM-T vector with subsequent sequence identification. The gene fragment was inserted into the prokaryotic expression vector pBV220 and transformed into E.coli DH5alpha strain. Endostatin expression in the E.coli was identified and the inclusion body isolated, purified and its activity analyzed.
  
  
  RESULTS
  The obtained gene fragment 552 bp in length was identified as the functional section of human endostatin gene by sequence analysis, and SDS-PAGE analysis showed that the expressed product was the target protein with biological activity.
  
  
  CONCLUSIONS
  Human endostatin gene was expressed in E.coli and the protein obtained can inhibit the proliferation of ECV 304 cells.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: He
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Jiang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Dong-Xian
authors.initials: DX
authors.lastname: Peng
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zong
mesh_terms: D003001:Cloning, Molecular; D043169:Endostatins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D043169:Endostatins