title: Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes.
abstract: BACKGROUND
  There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD.
  
  
  METHODS
  Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2'-deoxyguanosine (8-OHdG).
  
  
  RESULTS
  Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes.
  
  
  CONCLUSIONS
  The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Chia-Chao
authors.initials: CC
authors.lastname: Wu
authors.forename: Jin-Shuen
authors.initials: JS
authors.lastname: Chen
authors.forename: Wen-Mein
authors.initials: WM
authors.lastname: Wu
authors.forename: Tung-Nan
authors.initials: TN
authors.lastname: Liao
authors.forename: Pauling
authors.initials: P
authors.lastname: Chu
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Lin
authors.forename: Chien-Huei
authors.initials: CH
authors.lastname: Chuang
authors.forename: Yuh-Feng
authors.initials: YF
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000097:blood*; D006435:Renal Dialysis; D013481:Superoxides / Q000097:blood
chemical_list: D015415:Biomarkers; D008567:Membranes, Artificial; D013481:Superoxides; D009195:Peroxidase