[High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium].
OBJECTIVE
To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC).


METHODS
ODS HYPERSIL C(18) column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used.


RESULTS
The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively; relative standard deviations (RSDs) of within-day and between-days were less than 1.5% and 5.1%, respectively.


CONCLUSIONS
The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium.
Miao
Yu
Y
Department of Cardiology, Xi yuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. miaoyu0609@sina.com
Wang
Cheng-long
CL
Yin
Hui-jun
HJ
Shi
Da-zhuo
DZ
Chen
Ke-ji
KJ
D000227:Adenine Nucleotides / Q000032:analysis; D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate