Determination of sterols in biological samples by SPME with on-fiber derivatization and GC/FID.
A new procedure for the determination of sterols in serum samples is proposed. The system consists of coating a Solid Phase Microextraction (SPME) microfiber in headspace mode with the derivatizing agent N,O-bis(trimethylsilyl)trifluoracetamide (BSTFA) and then applying this coated fiber to the simultaneous extraction and derivatization of three precursors in the cholesterol biosynthesis pathway (desmosterol, lathosterol and lanosterol) and two phytosterols (sitosterol and sitostanol) in serum samples. Optimization of the analytical procedure via the application of an experimental design, a study of matrix effects, and an analysis of serum pool samples are all described and discussed.
Domeño
Celia
C
Department of Analytical Chemistry, Centro Politécnico Superior (CPS), University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain.
Ruiz
Bárbara
B
Nerín
Cristina
C
D005410:Flame Ionization; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013261:Sterols / Q000032:analysis* / Q000097:blood; D014297:Trimethylsilyl Compounds
D007202:Indicators and Reagents; D013261:Sterols; D014297:Trimethylsilyl Compounds; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide