title: G2 arrest and apoptosis by 2-amino-N-quinoline-8-yl-benzenesulfonamide (QBS), a novel cytotoxic compound.
abstract: We screened a library of 11,000 small molecular weight chemicals, looking for compounds that affect cell viability. We have identified 2-amino-N-quinoline-8-yl-benzenesulfonamide (QBS) as a potent cytotoxic compound that induces cell cycle arrest and apoptosis. Treatment of Jurkat T cells with QBS increased the levels of cyclin B1 as well as phosphorylated-cdc2, which was accompanied by reduced activity of cdc2 kinase, suggesting that QBS may induce cell cycle arrest at G2 phase. Structural analogues of QBS also exhibited similar effects on cell cycle progression and cell viability. Long-term treatment with QBS resulted in DNA fragmentation, cytochrome C release, and PARP cleavage, and an increase in the number of subdiploidy cells, indicative of cellular apoptosis. Moreover, QBS-induced apoptosis was blocked by z-VAD-fmk, a pan-caspase inhibitor. These results suggest that QBS is a novel and potent compound that induces G2 arrest and subsequent apoptosis, implicating it as a putative candidate for chemotherapy.
authors.affiliation: Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31 Hyojadong, Nam-Gu, Pohang, Kyungbuk 790-784, Republic of Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Kum-Joo
authors.initials: KJ
authors.lastname: Shin
authors.forename: Taehoon G
authors.initials: TG
authors.lastname: Lee
authors.forename: Euikyung
authors.initials: E
authors.lastname: Kim
authors.forename: Myoung-Shik
authors.initials: MS
authors.lastname: Lee
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Ryu
authors.forename: Pann-Ghill
authors.initials: PG
authors.lastname: Suh
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001554:Benzene / Q000737:chemistry / Q000633:toxicity*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D019169:Jurkat Cells; D011419:Propidium; D011804:Quinolines / Q000737:chemistry / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D005456:Fluorescent Dyes; D011804:Quinolines; D011419:Propidium; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D016650:Fluorescein-5-isothiocyanate; D001554:Benzene