[Analysis of cytochrome c oxidase I and cytochrome b genes of F1 in laboratory line Oncomelania hupensis hupensis].
OBJECTIVE
To analyze the diversity of F1 in laboratory line Oncomelania hupensis hupensis.


METHODS
Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes were amplified by polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed by GENETYX-MAC software package (ver. 9).


RESULTS
Pairwise divergences among six F1 individuals were found in 12.2% nucleotides in COI gene fragments. 13.5% of genetic divergences between O. h. hupensis and O. h. robertsoni were identified. 94 amino acids were observed in difference. In Cytb gene fragments, 6.4% of divergence was observed among four F1 samples. 25 amino acids were observed differently. The divergence of Cytb genes from O. h. robertsoni and O. h. hupensis were 13.6%, included 6 amino acids.


CONCLUSIONS
Diversities were found in both COI and Cytb gene sequences of F1 in laboratory line O. h. hupensis.
Zhang
Yi
Y
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Yamasaki
Hiroshi
H
Liu
He-xiang
HX
Feng
Ting
T
Feng
Zheng
Z
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012908:Snails / Q000145:classification / Q000201:enzymology* / Q000235:genetics*
D045303:Cytochromes b; D003576:Electron Transport Complex IV