[Mechanism of dracorhodin perchlorate-induced Hela cell apoptosis].
OBJECTIVE
To study the mechanism of dracorhodin perchlorate-induced Hela cell apoptosis.


METHODS
Cell viability was measured by MTT method. Morphological changes were observed by phase contrast microscopy and Hoechst 33258 staining. DNA fragmentation was assayed by agarose gel electrophoresis. Protein expression was detected by Western blot analysis.


RESULTS
Dracorhodin perchlorate induced Hela cell apoptosis. The apoptosis was partially reversed by caspase-1, -3, -8, -9 and caspase family inhibitors. Treatment of Hela cells with dracorhodin perchlorate for 12 h increased the protein expression ratio of Bax/Bcl-XL; procaspase-3, -8, ICAD and PARP were cleaved to smaller molecules.


CONCLUSIONS
Dracorhodin perchlorate induced Hela cell death via alteration of Bax/Bcl-XL ratio and activation of caspases.
Xia
Ming-yu
MY
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Min-wei
MW
Wang
Hao-ran
HR
Tashiro
Shin-ichi
S
Ikejima
Takashi
T
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D028023:Arecaceae / Q000737:chemistry; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry; D011506:Proteins / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D001578:Benzopyrans; D061945:Caspase Inhibitors; D004365:Drugs, Chinese Herbal; D011506:Proteins; C109664:caspase-activated DNase inhibitor; C060327:dracorhodin; D020169:Caspases