title: Osteolytic osteomyelitis associated with visceral leishmaniasis in a dog.
abstract: A dog was examined with a history of weight loss and lameness of the left hind limb. A painful response to examination of the left hip joint, and lymphadenopathy were noted. Amastigote forms of Leishmania sp. were observed by cytology in samples from the popliteus lymph node, and anti-Leishmania sp. antibodies at a titer of 1:640 were detected in serum by indirect immunofluorescence. Radiological changes included osteolysis and a periosteal proliferative reaction in the left femoral greater trochanter. These changes were histologically characterized as an osteolytic granulomatous osteomyelitis associated with amastigotes within macrophages. Non-decalcified fragments of the periosteum were processed for immunohistochemistry, observed with prominent immunolabelling of amastigotes of Leishmania sp. within macrophages. The diagnosis was further confirmed by positive PCR for Leishmania sp., belonging to the Leishmania donovani complex.
authors.affiliation: Curso de Medicina Veterinária, Centro de Ciências Biológicas, Agrárias e da Saúde (CCBAS), Universidade para o Desenvolvimento do Estado e da Região do Pantanal (UNIDERP), Rua Alexandre Herculano 1400, 79037-280 Campo Grande, MS, Brazil. alda@nin.ufms.br
authors.forename: Alda Izabel
authors.initials: AI
authors.lastname: de Souza
authors.forename: Raquel Soares
authors.initials: RS
authors.lastname: Juliano
authors.forename: Tatiana Scanoni
authors.initials: TS
authors.lastname: Gomes
authors.forename: Soraia
authors.initials: S
authors.lastname: de Araujo Diniz
authors.forename: Alan Maia
authors.initials: AM
authors.lastname: Borges
authors.forename: Wagner Luiz
authors.initials: WL
authors.lastname: Tafuri
authors.forename: Renato Lima
authors.initials: RL
authors.lastname: Santos
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D004285:Dogs; D017809:Fatal Outcome; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006614:Hindlimb / Q000000981:diagnostic imaging / Q000469:parasitology / Q000473:pathology; D007893:Leishmania donovani / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D008264:Macrophages / Q000469:parasitology; D008297:Male; D010019:Osteomyelitis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D011859:Radiography