title: [Inhibition of HBV core antigen gene expression in human embryonic kidney cell line AD293 by plasmid-based RNAi].
abstract: To inhibit HBV core antigen gene expression with plasmid-based RNAi.
  
  
  
  The shRNA expression vector targeting HBV core antigen gene was designed and constructed. Human embryonic kidney cell line AD293 was co-transfected with HBcAg-EGFP fusion protein expression vector and shRNA expression vector transiently, and the cells without shRNA-transfection and with non-specific shRNA transfection were used as controls. Inhibitory effect of RNAi was detected by fluorescence-activated cell sorting (FACS) and real-time fluorescence quantificational RT-PCR.
  
  
  
  HBV core antigen gene expression in AD293 was inhibited by shRNA, with the maximal inhibition rate of 76 % measured by FACS and of 63.1 % by real-time PCR.
  
  
  
  Effective inhibition of HBV core antigen gene expression by plasmid-based RNAi provides an alternative for anti-HBV study in vitro, which has potential clinical application.
authors.affiliation: Institute of Infectious Diseases, The First Affiliated Hospital, Key Laboratory of Infectious Diseases Ministry of Health, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Zhu
authors.forename: Hang-ping
authors.initials: HP
authors.lastname: Yao
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Hou
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D004622:Embryo, Mammalian; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D034622:RNA Interference / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D006512:Hepatitis B Core Antigens; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins