title: [Biological features of mouse macrophage transfected with Toxoplasma gondii GRA-1 gene].
abstract: OBJECTIVE
  To investigate the change of biological features of macrophages after transfected by Toxoplasma gondii GRA-1 genes (P24).
  
  
  METHODS
  The transfected cells Cyto-P24-RAW264.7, Nuc-P24-RAW264.7, Mito-P24-RAW264.7 and ER-P24-RAW264.7 were studied by RT-PCR to determine the P24-mRNA expression. Growth features of the cells were examined with microscopy and the cell growth curve was developed.
  
  
  RESULTS
  In four cell lines, expression of ER-P24-RAW 264.7 was found to be higher than the other three, and there was no P24-mRNA expression in either of the cells without P24 insert. The attachment and the proliferation of ER-P24-RAW264.7 were more rapid than normal RAW264.7.
  
  
  CONCLUSIONS
  Transfection of mouse macrophages ER-RAW264. 7 strain with T. gondii P24 gene leads to a prominent change of biological features in the studied cell line.
authors.affiliation: Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Bi-wen
authors.initials: BW
authors.lastname: Peng
authors.forename: Jian-yin
authors.initials: JY
authors.lastname: Lin
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Ming-sen
authors.initials: MS
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014122:Toxoplasma / Q000235:genetics*; D014162:Transfection
chemical_list: D000953:Antigens, Protozoan; C412913:GRA1 antigen; D012333:RNA, Messenger