title: Modulation of cytokine production by Porphyromonas gingivalis in a macrophage and epithelial cell co-culture model.
abstract: Epithelial cells and macrophages play a major role in the host response to Porphyromonas gingivalis, a major etiologic agent of chronic periodontitis. Secretion of high levels of cytokines by these cells is believed to contribute to periodontal tissue destruction. To investigate the interactions between P. gingivalis and these two major cell types, we characterized the production of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and regulated on activation normal T cell expressed and secreted (RANTES) by an in vitro co-culture model composed of epithelial-like transformed cells (HeLa cell line) and macrophage-like cells (phorbol myristic acid-differentiated U937 cell line) following a challenge with different strains of P. gingivalis. P. gingivalis cells stimulated the secretion of pro-inflammatory cytokines (IL-1beta and IL-6) and chemokines (IL-8 and RANTES) in the co-culture model. Responses to P. gingivalis infection were influenced by the macrophage/epithelial cell ratios of the cultures. In addition, the level of secretion of these inflammatory mediators was dependent on the bacterial strain and the multiplicity of infection (MOI) used. The use of a gingipain-deficient mutant of P. gingivalis or the addition of a cysteine protease inhibitor suggested that the level of cytokines secreted by the co-culture model was underestimated due to an extensive proteolytic degradation. This study showed that P. gingivalis can modulate the levels of inflammatory mediators, which may contribute to the progression of periodontitis.
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Que., Canada, G1K 7P4.
authors.forename: Charles
authors.initials: C
authors.lastname: Bodet
authors.forename: Fatiha
authors.initials: F
authors.lastname: Chandad
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenier
mesh_terms: D018920:Coculture Techniques; D003546:Cysteine Endopeptidases / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000276:immunology*
chemical_list: D016207:Cytokines; D003546:Cysteine Endopeptidases