Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol-gel-glued cyclodextrin-modified silica particles.
By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kV) and pressure (300 bar) and possesses a high efficiency (up to 100,000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.
Wistuba
Dorothee
D
Institute of Organic Chemistry, University of Tübingen, D-72076 Tübingen, Germany. dorothee.wistuba@uni-tuebingen.de
Banspach
Leena
L
Schurig
Volker
V
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D005782:Gels; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
D003505:Cyclodextrins; D005782:Gels; D012822:Silicon Dioxide