title: Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022.
abstract: The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
authors.affiliation: Department of Biochemistry, Tel-Aviv University, Israel.
authors.forename: Pnina
authors.initials: P
authors.lastname: Gottfried
authors.forename: Ofra
authors.initials: O
authors.lastname: Lotan
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kolot
authors.forename: Ludmila
authors.initials: L
authors.lastname: Maslenin
authors.forename: Rosa
authors.initials: R
authors.lastname: Bendov
authors.forename: Rena
authors.initials: R
authors.lastname: Gorovits
authors.forename: Vered
authors.initials: V
authors.lastname: Yesodi
authors.forename: Ezra
authors.initials: E
authors.lastname: Yagil
authors.forename: Arie
authors.initials: A
authors.lastname: Rosner
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D025523:Bacteriophage HK022 / Q000201:enzymology*; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D019426:Integrases