title: Ki-67 expression in benign breast ductal cells obtained by random periareolar fine needle aspiration.
abstract: Ki-67 expression in ductal cells obtained by random periareolar fine needle aspiration (RPFNA) is currently being used as a response biomarker in phase II breast cancer chemoprevention trials; however, Ki-67 in RPFNA has not been well studied as a risk predictor for cancer, which would support its use as a response indicator. We examined the expression of Ki-67 in RPFNA specimens with hyperplasia +/- atypia obtained from 147 women at high risk for development of breast cancer. Median Ki-67 was 1.4% (range 0-24%). Ki-67 was higher in specimens from women < 50 versus those > or = 50 (median 2% versus 0.6%; P = 0.006) and from premenopausal women versus postmenopausal women (P = 0.037); however, hormone replacement therapy (predominately low-dose estrogen without progestins) had no effect. By univariate analysis, Ki-67 was positively correlated with ductal cell number (P = 0.001) and hyperplasia with atypia (P = 0.007). By multivariable analysis, the proportion of ductal cells expressing Ki-67 was again predicted by cell number, which, in turn, was predicted by cytologic atypia. The association of Ki-67 expression with cytologic atypia, a known risk factor for development of breast cancer, provides preliminary justification for its use as a response biomarker in phase II chemoprevention trials.
authors.affiliation: University of Kansas Medical Center, Kansas City, KS 66160, USA.
authors.forename: Qamar J
authors.initials: QJ
authors.lastname: Khan
authors.forename: Bruce F
authors.initials: BF
authors.lastname: Kimler
authors.forename: Julie
authors.initials: J
authors.lastname: Clark
authors.forename: Trina
authors.initials: T
authors.lastname: Metheny
authors.forename: Carola M
authors.initials: CM
authors.lastname: Zalles
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Fabian
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000145:classification / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000473:pathology* / Q000517:prevention & control; D018270:Carcinoma, Ductal, Breast / Q000473:pathology* / Q000517:prevention & control; D015331:Cohort Studies; D005260:Female; D020249:Hormone Replacement Therapy; D006801:Humans; D006965:Hyperplasia; D019394:Ki-67 Antigen / Q000302:isolation & purification*; D008593:Menopause; D008875:Middle Aged; D017698:Postmenopause
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen