title: The cellular uptake of sensitizers bound to cyclodextrin carriers.
abstract: Photodynamic therapy of cancer uses the interaction of sensitizers and light to destroy cancer cells. In this study we tested the cellular uptake of meso-tetrakis(4-sulfonatophenyl)porphine (TPPS4) and its complex PdTPPS4 in the presence or absence of 2-hydroxypropyl-cyclodextrins (hpCDs) on G361 human melanoma cells. Self-fluorescence in G361 cells were measured by Perkin-Elmer LS50B luminometer equipped with well plate reader accessory. Morphological changes in cells have been evaluated using inversion fluorescent microscope Olympus IX 70 and image analysis. The uptake of the sensitizer PdTPPS4 at the given time interval from 1 to 48 hours is markedly higher than the uptake of TPPS4. The highest uptake was found for sensitizer PdTPPS4 in combination with hpbetaCD. TPPS4 and PdTPPS4 especially in the supramolecular complex with nontoxic cyclodextrin carriers represent efficient sensitizers for photodynamic therapy in vitro on G361 cells.
authors.affiliation: Department of Biophysics, Faculty of Medicine, Palacky University Olomouc, Czech Republic. kol@tunw.upol.cz
authors.forename: Hana
authors.initials: H
authors.lastname: Kolárová
authors.forename: Martin
authors.initials: M
authors.lastname: Huf
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Macedek
authors.forename: Pavla
authors.initials: P
authors.lastname: Nevrelová
authors.forename: Marek
authors.initials: M
authors.lastname: Tomecka
authors.forename: Robert
authors.initials: R
authors.lastname: Bajgar
authors.forename: Jirí
authors.initials: J
authors.lastname: Mosinger
authors.forename: Miroslav
authors.initials: M
authors.lastname: Strnad
mesh_terms: D045744:Cell Line, Tumor / Q000378:metabolism; D003505:Cyclodextrins / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D008546:Melanoma, Experimental / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010165:Palladium; D011166:Porphyrins / Q000493:pharmacokinetics*; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*; D012878:Skin Neoplasms / Q000378:metabolism*
chemical_list: D003505:Cyclodextrins; D004337:Drug Carriers; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C016136:tetraphenylporphine sulfonate; D010165:Palladium