Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.
OBJECTIVE
To report the first birth after transfer of cryopreserved embryos generated by intracytoplasmic sperm injection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.


METHODS
Case report.


METHODS
Tertiary center for reproductive technology.


METHODS
A 36-year-old woman with primary infertility of 3 years' duration and a 37-year-old man with congenital bilateral absence of the vas deferens.


METHODS
Cryopreservation of human embryos after oocytes and sperm thawing.


METHODS
Live birth.


RESULTS
A healthy, normal female infant with a birth weight of 2,950 g was born by cesarean section at 38 weeks' gestation, with normal 6-month follow-up.


CONCLUSIONS
Embryo cryopreservation can lead to successful results, even with the use of cryopreserved gametes.
Levi Setti
Paolo Emanuele
PE
Operative Unit Reproductive Medicine, Istituto Clinico Humanitas, Rozzano (Milan), Italy. paolo.levi_setti@humanitas.it
Albani
Elena
E
Novara
Paola Vittoria
PV
Cesana
Amalia
A
Bianchi
Silvia
S
Negri
Luciano
L
D000328:Adult; D001724:Birth Weight; D002585:Cesarean Section; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005500:Follow-Up Studies; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology / Q000637:transplantation*; D014649:Vas Deferens / Q000002:abnormalities / Q000502:physiology