title: Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis.
abstract: Myosin VI plays a role in the maintenance of Golgi morphology and in exocytosis. In a yeast 2-hybrid screen we identified optineurin as a binding partner for myosin VI at the Golgi complex and confirmed this interaction in a range of protein interaction studies. Both proteins colocalize at the Golgi complex and in vesicles at the plasma membrane. When optineurin is depleted from cells using RNA interference, myosin VI is lost from the Golgi complex, the Golgi is fragmented and exocytosis of vesicular stomatitis virus G-protein to the plasma membrane is dramatically reduced. Two further binding partners for optineurin have been identified: huntingtin and Rab8. We show that myosin VI and Rab8 colocalize around the Golgi complex and in vesicles at the plasma membrane and overexpression of constitutively active Rab8-Q67L recruits myosin VI onto Rab8-positive structures. These results show that optineurin links myosin VI to the Golgi complex and plays a central role in Golgi ribbon formation and exocytosis.
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, England, UK.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Sahlender
authors.forename: Rhys C
authors.initials: RC
authors.lastname: Roberts
authors.forename: Susan D
authors.initials: SD
authors.lastname: Arden
authors.forename: Giulietta
authors.initials: G
authors.lastname: Spudich
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Taylor
authors.forename: J Paul
authors.initials: JP
authors.lastname: Luzio
authors.forename: John
authors.initials: J
authors.lastname: Kendrick-Jones
authors.forename: Folma
authors.initials: F
authors.lastname: Buss
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells; D018797:Cell Cycle Proteins; D002645:Chickens; D006224:Cricetinae; D005089:Exocytosis; D015870:Gene Expression; D006056:Golgi Apparatus / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D000071058:Huntingtin Protein; D008562:Membrane Glycoproteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D018995:Myosin Heavy Chains / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D035582:Transcription Factor TFIIIA / Q000235:genetics / Q000378:metabolism*; D022161:Transport Vesicles / Q000502:physiology; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C028391:G protein, vesicular stomatitis virus; C086055:HTT protein, human; D000071058:Huntingtin Protein; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C409460:OPTN protein, human; D035582:Transcription Factor TFIIIA; D014759:Viral Envelope Proteins; C089785:myosin VI; C082377:RAB8A protein, human; D018995:Myosin Heavy Chains; D020691:rab GTP-Binding Proteins