title: Influence of the membrane lipid structure on signal processing via G protein-coupled receptors.
abstract: We have recently reported that lipid structure regulates the interaction with membranes, recruitment to membranes, and distribution to membrane domains of heterotrimeric Galphabetagamma proteins, Galpha subunits, and Gbetagamma dimers (J Biol Chem 279:36540-36545, 2004). Here, we demonstrate that modulation of the membrane structure not only determines G protein localization but also regulates the function of G proteins and related signaling proteins. In this context, the antitumor drug daunorubicin (daunomycin) and oleic acid changed the membrane structure and inhibited G protein activity in biological membranes. They also induced marked changes in the activity of the alpha(2A/D)-adrenergic receptor and adenylyl cyclase. In contrast, elaidic and stearic acid did not change the activity of the above-mentioned proteins. These fatty acids are chemical but not structural analogs of oleic acid, supporting the structural basis of the modulation of membrane lipid organization and subsequent regulation of G protein-coupled receptor signaling. In addition, oleic acid (and also daunorubicin) did not alter G protein activity in a membrane-free system, further demonstrating the involvement of membrane structure in this signal modulation. The present work also unravels in part the molecular bases involved in the antihypertensive (Hypertension 43:249-254, 2004) and anticancer (Mol Pharmacol 67:531-540, 2005) activities of synthetic oleic acid derivatives (e.g., 2-hydroxyoleic acid) as well as the molecular bases of the effects of diet fats on human health.
authors.affiliation: Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Regina
authors.initials: R
authors.lastname: Alemany
authors.forename: Jesús
authors.initials: J
authors.lastname: Casas
authors.forename: Klára
authors.initials: K
authors.lastname: Kitajka
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Lanier
authors.forename: Pablo V
authors.initials: PV
authors.lastname: Escribá
mesh_terms: D000818:Animals; D000068438:Brimonidine Tartrate; D004305:Dose-Response Relationship, Drug; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D011810:Quinoxalines / Q000378:metabolism / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D008563:Membrane Lipids; D011810:Quinoxalines; D043562:Receptors, G-Protein-Coupled; D000068438:Brimonidine Tartrate