title: Evaluation of the phospholamban gene in purebred large-breed dogs with dilated cardiomyopathy.
abstract: OBJECTIVE
  To evaluate the role of the phospholamban gene in purebred large-breed dogs with dilated cardiomyopathy (DCM).
  
  
  METHODS
  6 dogs with DCM, including 2 Doberman Pinschers, 2 Newfoundlands, and 2 Great Danes.
  
  
  METHODS
  All dogs had clinical signs of congestive heart failure, and a diagnosis of DCM was made on the basis of echocardiographic findings. Blood samples were collected from each dog, and genomic DNA was isolated by a salt extraction method. Specific oligonucleotides were designed to amplify the promoter, exon 1, the 5'-part of exon 2 including the complete coding region, and part of intron 1 of the canine phospholamban gene via polymerase chain reaction procedures. These regions were screened for mutations in DNA obtained from the 6 dogs with DCM.
  
  
  RESULTS
  No mutations were identified in the promoter, 5' untranslated region, part of intron 1, part of the 3' untranslated region, and the complete coding region of the phospholamban gene in dogs with DCM.
  
  
  CONCLUSIONS
  Results indicate that mutations in the phospholamban gene are not a frequent cause of DCM in Doberman Pinschers, Newfoundlands, and Great Danes.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
authors.forename: Polona
authors.initials: P
authors.lastname: Stabej
authors.forename: Peter A
authors.initials: PA
authors.lastname: Leegwater
authors.forename: Arnold A
authors.initials: AA
authors.lastname: Stokhof
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Domanjko-Petric
authors.forename: Bernard A
authors.initials: BA
authors.lastname: van Oost
mesh_terms: D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002311:Cardiomyopathy, Dilated / Q000235:genetics / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D004452:Echocardiography / Q000662:veterinary; D004562:Electrocardiography / Q000662:veterinary; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D017422:Sequence Analysis, DNA / Q000662:veterinary; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D009841:Oligonucleotides; D000099211:Phospholamban