Mutation analysis of the preproghrelin gene: no association with obesity and type 2 diabetes.
OBJECTIVE
To investigate the preproghrelin gene for variants and their association with obesity and type 2 diabetes.


METHODS
82 obese probands were analyzed for mutations using single-strand conformational polymorphism, heteroduplex analyses and sequencing. Association studies were performed in 234 juvenile-onset obese and 323 lean men and in 557 type 2 diabetic and 233 glucose tolerant subjects.


RESULTS
We identified two novel variants, 36C > T and IVS3 + 715delC, and 4 known variants, Arg51Gln, Leu72Met, Gln90Leu, and IVS1 + 169G > A. None of the variants showed any significant association with obesity or type 2 diabetes or estimates of glucose and lipid metabolism in glucose tolerant subjects.


CONCLUSIONS
Variation in the preproghrelin gene is not associated with juvenile-onset obesity, type 2 diabetes or related phenotypes among the examined Danish Caucasian subjects.
Larsen
Lesli H
LH
Steno Diabetes Center and Hagedorn Research Institute, Copenhagen, Denmark. LeiL@steno.dk
Gjesing
Anette P
AP
Sørensen
Thorkild I A
TI
Hamid
Yasmin H
YH
Echwald
Søren M
SM
Toubro
Søren
S
Black
Eva
E
Astrup
Arne
A
Hansen
Torben
T
Pedersen
Oluf
O
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D004252:DNA Mutational Analysis / Q000379:methods; D003718:Denmark; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005838:Genotype; D054439:Ghrelin; D006801:Humans; D008297:Male; D008875:Middle Aged; D009037:Motilin / Q000235:genetics*; D009765:Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic
D054439:Ghrelin; D009037:Motilin