title: Immunogenicity of two Echinococcus granulosus antigens EgA31 and EgTrp in mice.
abstract: Here, we investigate in mice the immunogenicity of two antigens EgA31 and EgTrp which are expressed by the larval stage of Echinococcus granulosus. These recombinant proteins were used alone or as a mixture (EgA31-EgTrp) to immunize BALB/c mice. By flow cytometry, we have shown that the ratio CD4+/CD8+ of splenocytes were significantly higher in the antigen-immunized groups. The specific antibody in the sera and cytokine producing splenocytes was evaluated by enzyme-linked immunosorbent assay. EgA31, EgTrp or EgA31-EgTrp elicited high antibody titer of IgG and IgA. Among IgG isotypes, IgG1 was predominant for each antigen tested alone or combined. The production of IL-12, IFN-gamma, IL-10 and IL-6 cytokines was significantly higher in mice immunized with recombinant proteins. Our results suggest that, in BALB/c mice, a mixed Th1/Th2, response to EgA31, EgTrp and EgA31-EgTrp is obtained. The use of both antigens separately or in combination as candidate vaccine proteins is discussed.
authors.affiliation: Laboratory of Parasitology and Medical Mycology EA3741, Faculty of Pharmacy, Claude-Bernard University, 8, avenue Rockefeller, 69373, Lyon cedex 08, France.
authors.forename: M
authors.initials: M
authors.lastname: Fraize
authors.forename: M E
authors.initials: ME
authors.lastname: Sarciron
authors.forename: S
authors.initials: S
authors.lastname: Azzouz
authors.forename: N
authors.initials: N
authors.lastname: Issaadi
authors.forename: G
authors.initials: G
authors.lastname: Bosquet
authors.forename: A F
authors.initials: AF
authors.lastname: Petavy
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000378:metabolism; D004443:Echinococcosis / Q000401:mortality / Q000517:prevention & control; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014612:Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; C401864:EgA31 protein, Echinococcus granulosus; D007074:Immunoglobulin G; D014612:Vaccines