[Association of interleukin-6 induction and the novel gene LX3].
OBJECTIVE
To analyze the association between the expression of the novel gene LX3 and interleukin-6 (IL-6) induction, and explore a new target gene for action mechanism of IL-6.


METHODS
The total RNA was extracted from U937 cells induced by IL-6 at different concentrations and varied lengths of time. Reverse transcriptional (RT)-PCR and Northern blotting were employed to determine the expression of LX3 and IL-6 induction.


RESULTS
The expression of novel gene LX3 was increased while concentration of IL-6 was improved gradually. High expression was induced by IL-6, which was highest at the concentration was 500 ng/ml and no expression at 0 ng/ml. Time- expression pattern proved that expression of novel gene LX3 of higest at 8 hour after induced by IL-6. Northern blotting confirmed that the expression quantity of LX3 increased in U937 cells induced by IL-6.


CONCLUSIONS
The quantity of LX3 expression is associated with the dose and duration of IL-6 induction, suggesting that LX3 gene is a novel gene correlated with IL-6 induction.
Xia
Rong
R
Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Li
Yan
Y
Feng
Jian-Nan
JN
Lan
Jiong-Cai
JC
Shen
Bei-Fen
BF
D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells
D015850:Interleukin-6