BSP A1/A2-like proteins in ram seminal plasma.
The objective of this study was to assess the protein profile of ovine seminal plasma using 2D-PAGE and verify if BSP A1/A2 are present in ovine seminal plasma. Seminal plasma was collected from three mature rams and pooled to eliminate individual differences. Seminal plasma samples were submitted to 2D-PAGE using 12% acrylamide gels. The image analysis software identified 21 protein spots on the air-dried gel, with molecular weight ranging from 15 to 115 kDa and pI 3.2 to 8.7. The most prominent spots were those <30 kDa. The most intensely stained spots were: 3 (18-19 kDa, pI 4.8-5.0), 5 (17-18 kDa, pI 5.0-5.2), 7 (15-16 kDa, pI 6.2-6.4), and 23 (105-108 kDa, pI 6.8-7.0). Three of these spots (spots 3, 5 and 7, respectively) accounted for 41.1% of the relative intensity of the spots of the gels, based on the intensity of the Comassie blue staining. Western blot analysis indicated that spots 3 and 5 were similar to BSP A1/A2 (16.5, pI 4.7-5.0 and 16 kDa, pI 4.9-5.2) identified in Manjunath's studies [Manjunath P, Sairam MR. Purification and biochimical characterization of three major acid proteins (BSP A1, BSP A2 and BSP A3) from bovine seminal plasma. Biochem J 7 (1987) 685-92.], based on the specific reaction of the polyclonal antibody to those spots.
Jobim
M I M
MI
Department of Veterinary Clinical Pathology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. ines.jobim@ufrgs.br
Oberst
E R
ER
Salbego
C G
CG
Wald
V B
VB
Horn
A P
AP
Mattos
R C
RC
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000737:chemistry / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*
D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins