Functional re-expression of CCR7 on CMV-specific CD8+ T cells upon antigenic stimulation.
During latency circulating human cytomegalovirus (CMV)-specific CD8(+) T cells do not express the chemokine receptor CCR7. We here show that antigen-specific stimulation in vitro with the specific CMV-peptide in combination with CMV-antigen, IL-2 or IL-21 induced re-expression of CCR7 on CMV-specific CD8(+) T cells. Although IL-15 induced strong proliferation of peptide-pulsed CMV-specific CD8(+) T cells, these cells did not re-express CCR7. CMV-specific cells that re-expressed CCR7 also expressed CD62L and were able to react to specific chemokine stimulation with changes in the cytoskeleton. In addition, activated CMV-specific cells specifically migrated towards a chemokine gradient in a transwell assay, with and without an endothelial cell monolayer. We conclude that antigenic stimulation induced functional re-expression of CCR7 which suggests that the migratory properties of virus-primed T cells are flexible and depend on the presence or absence of antigen and cytokines.
van Leeuwen
Ester M M
EM
Department of Experimental Immunology, Avademic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. e.m.vanleeuwen@amc.uva.nl
van Buul
Jaap D
JD
Remmerswaal
Ester B M
EB
Hordijk
Peter L
PL
ten Berge
Ineke J M
IJ
van Lier
Rene A W
RA
D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000494:pharmacology; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D019041:L-Selectin / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism* / Q000821:virology
D000956:Antigens, Viral; C515460:CCL19 protein, human; C515478:CCL21 protein, human; C515370:CCR7 protein, human; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC; D007378:Interleukins; D010446:Peptide Fragments; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D019041:L-Selectin