Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy.
Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell.
Singh
Gajendra P
GP
ICFO-Institut de Ciències Fotoniques, Jordi Girona 29, Nexus II, 08034 Barcelona, Spain.
Creely
Caitriona M
CM
Volpe
Giovanni
G
Grötsch
Helga
H
Petrov
Dmitri
D
D000431:Ethanol / Q000032:analysis*; D005990:Glycerol / Q000032:analysis*; D006358:Hot Temperature; D052898:Optical Tweezers; D055095:Optics and Photonics; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000431:Ethanol; D005990:Glycerol