title: Complete nucleotide sequence of the RNA-2 of grapevine deformation and Grapevine Anatolian ringspot viruses.
abstract: The nucleotide sequence of RNA-2 of Grapevine Anatolian ringspot virus (GARSV) and Grapevine deformation virus (GDefV), two recently described nepoviruses, has been determined. These RNAs are 3753 nt (GDefV) and 4607 nt (GARSV) in size and contain a single open reading frame encoding a polyprotein of 122 kDa (GDefV) and 150 kDa (GARSV). Full-length nucleotide sequence comparison disclosed 71-73% homology between GDefV RNA-2 and that of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), and 62-64% homology between GARSV RNA-2 and that of Grapevine chrome mosaic virus (GCMV) and Tomato black ring virus (TBRV). As previously observed in other nepoviruses, the 5' non-coding regions of both RNAs are capable of forming stem-loop structures. Phylogenetic analysis of the three proteins encoded by RNA-2 (i.e. protein 2A, movement protein and coat protein) confirmed that GDefV and GARSV are distinct viruses which can be assigned as definitive species in subgroup A and subgroup B of the genus Nepovirus, respectively.
authors.affiliation: Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy. naboughanem@ucdavis.edu
authors.forename: Nina Abou
authors.initials: NA
authors.lastname: Ghanem-Sabanadzovic
authors.forename: Sead
authors.initials: S
authors.lastname: Sabanadzovic
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
authors.forename: Giovanni P
authors.initials: GP
authors.lastname: Martelli
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017860:Nepovirus / Q000145:classification / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D020815:Polyproteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins; C400561:protein 2A, nepovirus