title: Fabrication of quantum dot-lectin conjugates as novel fluorescent probes for microscopic and flow cytometric identification of leukemia cells from normal lymphocytes.
abstract: The present study describes a synthesis of QD-lectin conjugates and their application for identification of leukaemia cells from normal lymphocytes using fluorescent confocal microscopy and flow cytometry. The results are compared with commercially available FITC-lectin.
authors.affiliation: Single-Molecule Bioanalysis Laboratory, AIST-Shikoku, 2217-14, Hayashi-cho, Takamatsu, Kagawa-ken, Japan.
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Rajan
authors.initials: R
authors.lastname: Jose
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuoka
authors.forename: Toshimi
authors.initials: T
authors.lastname: Nagase
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D037102:Lectins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013677:Medical Laboratory Science; D018613:Microscopy, Confocal; D015394:Molecular Structure; D045663:Quantum Dots
chemical_list: D005456:Fluorescent Dyes; D037102:Lectins; D016650:Fluorescein-5-isothiocyanate