title: Application of isotope coded affinity tag (ICAT) analysis for the identification of differentially expressed proteins following infection of atlantic salmon (Salmo salar) with infectious hematopoietic necrosis virus (IHNV) or Renibacterium salmoninarum (BKD).
abstract: Aquaculture and commercial fisheries worldwide suffer from significant economic loss due to diseases of net-pen reared fish. In British Columbia, infectious hematopoietic necrosis (IHN) and bacterial kidney disease (BKD) epidemics occur because there are currently no commercially available drugs or fully licensed vaccines to treat these diseases. With a better understanding of the molecular mechanisms underlying these diseases, this circumstance might be significantly improved. In the present study, we have used a proteomics approach in an effort to identify and quantitate differentially expressed proteins in the liver and kidneys of diseased and healthy Atlantic salmon (Salmo salar). Isotope coded affinity tagging (ICAT), 2D gel electrophoresis, and multidimensional liquid chromatography coupled to tandem mass spectrometry (2D-LC MS/MS) were used to identify hundreds of differentially expressed proteins. While the direct significance of changes in expression levels of many proteins remains to be elucidated, others appear to be more clearly related to the infectious process. Examples of the latter are discussed here and include, a natural killer cell enhancement factor (NKEF), procathepsin L, superoxide-producing NADPH oxidase and interferon-induced viral resistance protein Mx (IFI-Mx).
authors.affiliation: University of Victoria, Genome British Columbia Proteomics Centre, #3101-4464 Markham Street, Victoria, British Columbia, Canada V8Z 7X8.
authors.forename: A T
authors.initials: AT
authors.lastname: Booy
authors.forename: J D
authors.initials: JD
authors.lastname: Haddow
authors.forename: L B
authors.initials: LB
authors.lastname: Ohlund
authors.forename: D B
authors.initials: DB
authors.lastname: Hardie
authors.forename: R W
authors.initials: RW
authors.lastname: Olafson
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D029142:Infectious hematopoietic necrosis virus / Q000472:pathogenicity*; D007554:Isotopes; D013058:Mass Spectrometry; D008835:Micrococcaceae / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism*; D020317:Salmo salar / Q000382:microbiology* / Q000821:virology*
chemical_list: D000345:Affinity Labels; D007554:Isotopes; D011506:Proteins