Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport.
Cortactin is an actin-binding protein that has recently been implicated in endocytosis. It binds directly to dynamin-2 (Dyn2), a large GTPase that mediates the formation of vesicles from the plasma membrane and the Golgi. Here we show that cortactin associates with the Golgi to regulate the actin- and Dyn2-dependent transport of cargo. Cortactin antibodies stain the Golgi apparatus, labelling peripheral buds and vesicles that are associated with the cisternae. Notably, in vitro or intact-cell experiments show that activation of Arf1 mediates the recruitment of actin, cortactin and Dyn2 to Golgi membranes. Furthermore, selective disruption of the cortactin-Dyn2 interaction significantly reduces the levels of Dyn2 at the Golgi and blocks the transit of nascent proteins from the trans-Golgi network, resulting in swollen and distended cisternae. These findings support the idea of an Arf1-activated recruitment of an actin, cortactin and Dyn2 complex that is essential for Golgi function.
Cao
Hong
H
Center for Basic Research in Digestive Diseases and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
Weller
Shaun
S
Orth
James D
JD
Chen
Jing
J
Huang
Bing
B
Chen
Ji-Long
JL
Stamnes
Mark
M
McNiven
Mark A
MA
D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D051356:Cortactin; D034285:Dynamin II / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D016253:Microscopy, Immunoelectron; D021381:Protein Transport / Q000502:physiology; D051381:Rats
D000199:Actins; D051356:Cortactin; C495870:Cttn protein, rat; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D020823:ADP-Ribosylation Factor 1; D034285:Dynamin II