title: Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India.
abstract: Larvae (15 to 21 d post hatch, dph) of the Asian sea bass Lates calcarifer (Bloch) suffered heavy mortalities (60 to 90%) during the hatchery-rearing phase. Darkened and moribund larvae showed no evidence of bacterial or parasitic infections. Tissue sections of brain and spinal cord showed clear necrotic vacuolation. Electron microscopy revealed membrane-bound viral particles in the cytoplasm of the nerve cells. The viral particles measured 28 to 30 nm in diameter. Primer sets, designed for the amplification of the RNA2 segment of the piscine nodavirus coat protein gene, were used in the RT-PCR analysis of moribund larvae of 20 and 21 dph which produced the amplified product of 430 bp. The clinical manifestations, pathology and electron microscopy observations supported by the RT-PCR analysis suggest that the nerve necrosis was due to nodavirus infection in the larvae. This is the first report of piscine nodavirus infection from the Indian sub-continent.
authors.affiliation: Central Institute of Brackishwater Aquaculture, 75-Santhome High Road, R. A. Puram, Chennai 600 028, India. azadis@rediffmail.com
authors.forename: I S
authors.initials: IS
authors.lastname: Azad
authors.forename: M S
authors.initials: MS
authors.lastname: Shekhar
authors.forename: A R
authors.initials: AR
authors.lastname: Thirunavukkarasu
authors.forename: M
authors.initials: M
authors.lastname: Poornima
authors.forename: M
authors.initials: M
authors.lastname: Kailasam
authors.forename: J J S
authors.initials: JJ
authors.lastname: Rajan
authors.forename: S A
authors.initials: SA
authors.lastname: Ali
authors.forename: M
authors.initials: M
authors.lastname: Abraham
authors.forename: P
authors.initials: P
authors.lastname: Ravichandran
mesh_terms: D000818:Animals; D017756:Aquaculture; D036022:Capsid Proteins / Q000235:genetics; D003593:Cytoplasm / Q000821:virology; D017931:DNA Primers; D005393:Fish Diseases / Q000453:epidemiology* / Q000401:mortality / Q000821:virology*; D007194:India / Q000453:epidemiology; D007814:Larva / Q000648:ultrastructure / Q000821:virology; D008854:Microscopy, Electron / Q000662:veterinary; D009474:Neurons / Q000648:ultrastructure / Q000821:virology; D030381:Nodaviridae; D010473:Perciformes; D012327:RNA Virus Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers