[Association of different alleles in interleukin-1 family genes with the coded cytokines production and outcome of sepsis].
OBJECTIVE
To investigate the association of alleles in interleukin-1(IL-1) family genes with IL-1alpha, IL-1beta, IL-1ra production as well as mRNA expression and the outcome of sepsis.


METHODS
Sixty septic patients and equal number of healthy volunteers were enrolled in this study. Monocytes of healthy volunteers segregated by Ficoll were stimulated by lipopolysaccharide (LPS) in vitro. IL-1alpha, IL-1beta and IL-1ra mRNA expressions were semi-quantitatively analyzed with reverse transcription-polymerase chain reaction (RT-PCR). Cytokine production was determined with enzyme linked immunoadsorbent assay(ELISA). Alleles in IL-1A (intron 6, VNTR), IL-1B (-511, RLFP) and IL-1RN (intron 2, VNTR) were screened in all cases.


RESULTS
The number of individuals carrying allele of IL-1RN2 was significantly greater in septic patients than that in healthy volunteers. Mortality in septic patients with IL-1RN2, compared with IL-1RN1, was markedly higher. Notably, IL-1ra production as well as mRNA expression of monocytes challenged with LPS was significantly higher in IL-1RN2 allele than in IL-1RN1 carriers. However, there was no difference in the coded gene expression between alleles of IL-1A1 and IL-1A2, also between IL-1B1 and IL-1B2.


CONCLUSIONS
IL-1RN2 allele induces high IL-1ra expression and is closely with the outcome of patients in sepsis. It could be one of high risk genetic factors in septic patients.
Yang
Ming-shi
MS
Affiliated the Third Hospital of Xiangya Medical College, Zhongnan University, Changsha 410013, Hunan, China. plma1019@yahoo.com
Ma
Peng-lin
PL
Chen
De-chang
DC
D000483:Alleles; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018598:Minisatellite Repeats; D009000:Monocytes / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D018805:Sepsis / Q000235:genetics* / Q000378:metabolism
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012333:RNA, Messenger