title: A linearization method for low catalytic activity enzyme kinetic analysis.
abstract: A kinetic analysis was made and a linear plot based on the general rate equation derived by Laidler [Can. J. Chem. 33, 1614-1624] is proposed. This linearization method allows determining the kinetic parameters (K(m), k(cat)) and [E](0) for enzymes with low catalytic activity. The method was applied to chloroperoxidase from Caldariomyces fumago [EC 1.11.1.10], whose kinetic parameters K(m)(app), k(cat)(app), and [E](0) with monochlorodimedone as substrate, were obtained by using the linearization plot and the V(max) value (calculated by Eadie-Hofstee plot). This plot could also be useful to the study of abenzyme kinetics provided the concentration of the latter is either higher or equal than K(m) value.
authors.affiliation: Department of Physiology and Biochemistry, Biochemistry Unit, University of Pisa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Toti
authors.forename: Antonella
authors.initials: A
authors.lastname: Petri
authors.forename: Valerio
authors.initials: V
authors.lastname: Pelaia
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Osman
authors.forename: Moreno
authors.initials: M
authors.lastname: Paolini
authors.forename: Carlo
authors.initials: C
authors.lastname: Bauer
mesh_terms: D002384:Catalysis; D002711:Chloride Peroxidase / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology; D007700:Kinetics; D016014:Linear Models
chemical_list: D002711:Chloride Peroxidase