title: TLR3-induced activation of mast cells modulates CD8+ T-cell recruitment.
abstract: Mast cells play an important role in host defense against various pathogens, but their role in viral infection has not been clarified in detail. dsRNA, synthesized by various types of viruses and mimicked by polyinosinic-polycytidylic acid (poly(I:C)) is recognized by Toll-like receptor 3 (TLR3). In this study, we demonstrate that poly(I:C) injection in vivo potently stimulates peritoneal mast cells to up-regulate a number of different costimulatory molecules. Therefore, we examined the expression and the functional significance of TLR3 activation in mast cells. Mast cells express TLR3 on the cell surface and intracellularly. After stimulation of mast cells with poly(I:C) and Newcastle disease virus (NDV), TLR3 is phosphorylated and the expression of key antiviral response cytokines (interferon beta, ISG15) and chemokines (IP10, RANTES) is upregulated. Interestingly, mast cells activated via TLR3-poly(I:C) potently stimulate CD8+ T-cell recruitment. Indeed, mast-cell-deficient mice (KitW/KitW-v) given an intraperitoneal injection of poly(I:C) show a decreased CD8+ T-cell recruitment, whereas granulocytes normally migrate to the peritoneal cavity. Mast-cell reconstitution of KitW/KitW-v mice normalizes the CD8+ T-cell influx. Thus, mast cells stimulated through engagement of TLR3 are potent regulators of CD8+ T-cell activities in vitro and in vivo.
authors.affiliation: Department of Immunology and Cellular Biology, Research Center Borstel, Parkallee 22, D-23845 Borstel, Germany.
authors.forename: Zane
authors.initials: Z
authors.lastname: Orinska
authors.forename: Elena
authors.initials: E
authors.lastname: Bulanova
authors.forename: Vadim
authors.initials: V
authors.lastname: Budagian
authors.forename: Martin
authors.initials: M
authors.lastname: Metz
authors.forename: Marcus
authors.initials: M
authors.lastname: Maurer
authors.forename: Silvia
authors.initials: S
authors.lastname: Bulfone-Paus
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000096:biosynthesis; D007113:Immunity, Innate; D008407:Mast Cells / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009522:Newcastle disease virus; D010529:Peritoneal Cavity; D010766:Phosphorylation; D011070:Poly I-C / Q000008:administration & dosage / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051196:Toll-Like Receptor 3; D051193:Toll-Like Receptors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D016207:Cytokines; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D051196:Toll-Like Receptor 3; D051193:Toll-Like Receptors; D011070:Poly I-C