Immunogenicity of two Echinococcus granulosus antigens EgA31 and EgTrp in mice.
Here, we investigate in mice the immunogenicity of two antigens EgA31 and EgTrp which are expressed by the larval stage of Echinococcus granulosus. These recombinant proteins were used alone or as a mixture (EgA31-EgTrp) to immunize BALB/c mice. By flow cytometry, we have shown that the ratio CD4+/CD8+ of splenocytes were significantly higher in the antigen-immunized groups. The specific antibody in the sera and cytokine producing splenocytes was evaluated by enzyme-linked immunosorbent assay. EgA31, EgTrp or EgA31-EgTrp elicited high antibody titer of IgG and IgA. Among IgG isotypes, IgG1 was predominant for each antigen tested alone or combined. The production of IL-12, IFN-gamma, IL-10 and IL-6 cytokines was significantly higher in mice immunized with recombinant proteins. Our results suggest that, in BALB/c mice, a mixed Th1/Th2, response to EgA31, EgTrp and EgA31-EgTrp is obtained. The use of both antigens separately or in combination as candidate vaccine proteins is discussed.
Fraize
M
M
Laboratory of Parasitology and Medical Mycology EA3741, Faculty of Pharmacy, Claude-Bernard University, 8, avenue Rockefeller, 69373, Lyon cedex 08, France.
Sarciron
M E
ME
Azzouz
S
S
Issaadi
N
N
Bosquet
G
G
Petavy
A F
AF
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000378:metabolism; D004443:Echinococcosis / Q000401:mortality / Q000517:prevention & control; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014612:Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; C401864:EgA31 protein, Echinococcus granulosus; D007074:Immunoglobulin G; D014612:Vaccines