Insights into hydrophobicity and the chaperone-like function of alphaA- and alphaB-crystallins: an isothermal titration calorimetric study.
Alpha-crystallin, composed of two subunits, alphaA and alphaB, has been shown to function as a molecular chaperone that prevents aggregation of other proteins under stress conditions. The exposed hydrophobic surfaces of alpha-crystallins have been implicated in this process, but their exact role has not been elucidated. In this study, we quantify the hydrophobic surfaces of alphaA- and alphaB-crystallins by isothermal titration calorimetry using 8-anilino-1-napthalenesulfonic acid (ANS) as a hydrophobic probe and analyze its correlation to the chaperone potential of alphaA- and alphaB-crystallins under various conditions. Two ANS binding sites, one with low and another with high affinity, were clearly detected, with alphaB showing a higher number of sites than alphaA at 30 degrees C. In agreement with the higher number of hydrophobic sites, alphaB-crystallin demonstrated higher chaperone activity than alphaA at this temperature. Thermodynamic analysis of ANS binding to alphaA- and alphaB-crystallins indicates that high affinity binding is driven by both enthalpy and entropy changes, with entropy dominating the low affinity binding. Interestingly, although the number of ANS binding sites was similar for alphaA and alphaB at 15 degrees C, alphaA was more potent than alphaB in preventing aggregation of the insulin B-chain. Although there was no change in the number of high affinity binding sites of alphaA and alphaB for ANS upon preheating, there was an increase in the number of low affinity sites of alphaA and alphaB. Preheated alphaA, in contrast to alphaB, exhibited remarkably enhanced chaperone activity. Our results indicate that although hydrophobicity appears to be a factor in determining the chaperone-like activity of alpha-crystallins, it does not quantitatively correlate with the chaperone function of alpha-crystallins.
Kumar
M Satish
MS
Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India.
Kapoor
Mili
M
Sinha
Sharmistha
S
Reddy
G Bhanuprakash
GB
D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002151:Calorimetry; D004229:Dithiothreitol / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D005822:Genetic Vectors / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007313:Insecta; D007328:Insulin / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D038202:alpha-Crystallin A Chain / Q000737:chemistry*; D038203:alpha-Crystallin B Chain / Q000737:chemistry*
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D007328:Insulin; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D038202:alpha-Crystallin A Chain; D038203:alpha-Crystallin B Chain; C027132:1-anilino-8-naphthalenesulfonate; D004229:Dithiothreitol