title: Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport.
abstract: Cortactin is an actin-binding protein that has recently been implicated in endocytosis. It binds directly to dynamin-2 (Dyn2), a large GTPase that mediates the formation of vesicles from the plasma membrane and the Golgi. Here we show that cortactin associates with the Golgi to regulate the actin- and Dyn2-dependent transport of cargo. Cortactin antibodies stain the Golgi apparatus, labelling peripheral buds and vesicles that are associated with the cisternae. Notably, in vitro or intact-cell experiments show that activation of Arf1 mediates the recruitment of actin, cortactin and Dyn2 to Golgi membranes. Furthermore, selective disruption of the cortactin-Dyn2 interaction significantly reduces the levels of Dyn2 at the Golgi and blocks the transit of nascent proteins from the trans-Golgi network, resulting in swollen and distended cisternae. These findings support the idea of an Arf1-activated recruitment of an actin, cortactin and Dyn2 complex that is essential for Golgi function.
authors.affiliation: Center for Basic Research in Digestive Diseases and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Shaun
authors.initials: S
authors.lastname: Weller
authors.forename: James D
authors.initials: JD
authors.lastname: Orth
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Chen
authors.forename: Mark
authors.initials: M
authors.lastname: Stamnes
authors.forename: Mark A
authors.initials: MA
authors.lastname: McNiven
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D051356:Cortactin; D034285:Dynamin II / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D016253:Microscopy, Immunoelectron; D021381:Protein Transport / Q000502:physiology; D051381:Rats
chemical_list: D000199:Actins; D051356:Cortactin; C495870:Cttn protein, rat; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D020823:ADP-Ribosylation Factor 1; D034285:Dynamin II