title: Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models.
abstract: In this study, the anti-inflammatory, analgesic, and antipyretic effects of 50, 100 and 200 mg/kg body weight of methanol extract obtained from Bauhinia racemosa stem bark, the so-called MEBR, were investigated. The effects of MEBR on the acute and chronic phases of inflammation were studied in carrageenan, dextran and mediators (histamine and serotonin)-induced paw oedema and cotton pellet-induced granuloma, respectively. Analgesic effect of MEBR was evaluated in acetic acid-induced writhing and hotplate tests. Antipyretic activity of MEBR was evaluated by yeast-induced hyperpyrexia in rats. The anti-oedema effect of MEBR was compared with 10 mg/kg of indomethacin orally. In acute phase of inflammation, a maximum inhibition of 44.9, 43.2, 44.8 and 45.9% (P<0.001) was noted at the dose of 200 mg/kg b.w. after 3h of treatment with MEBR in carrageenan, dextran, histamine and serotonin-induced paw oedema, respectively. Administration of MEBR (200 mg/kg b.w.) and indomethacin (10 mg/kg b.w.) significantly (P<0.05) decreased the formation of granuloma tissue induced by cotton pellet method at a rate of 50.4 and 56.2%, respectively. The extract also inhibited peritoneal leukocyte migration in mice. The MEBR also produced significant (P<0.01) analgesic activity in both models. Further, the MEBR potentiated the morphine- and aspirin-induced analgesic in mice. Treatment with MEBR showed a significant (P<0.01) dose-dependent reduction in pyrexia in rats. The results suggest that MEBR possess potent anti-inflammatory, analgesic and antipyretic activity.
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India.
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: U K
authors.initials: UK
authors.lastname: Mazumder
authors.forename: R Sambath
authors.initials: RS
authors.lastname: Kumar
authors.forename: P
authors.initials: P
authors.lastname: Gomathi
authors.forename: Y
authors.initials: Y
authors.lastname: Rajeshwar
authors.forename: B B
authors.initials: BB
authors.lastname: Kakoti
authors.forename: V Tamil
authors.initials: VT
authors.lastname: Selven
mesh_terms: D000698:Analgesia; D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000302:isolation & purification / Q000627:therapeutic use / Q000633:toxicity*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use / Q000633:toxicity*; D036481:Bauhinia; D004487:Edema / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy; D007928:Lethal Dose 50; D008297:Male; D000432:Methanol / Q000302:isolation & purification; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use / Q000633:toxicity*; D051381:Rats
chemical_list: D000700:Analgesics; D018712:Analgesics, Non-Narcotic; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D000432:Methanol