[Combined use of autologous micro-morselized bone with bone morphogenetic protein and type I collagen graft in repairing rabbit bone defects].
OBJECTIVE
To study the effect of combined use of autologous micro-morselized bone with bone morphogenetic protein(BMP) and type I collagen graft on the treatment of segmental bone defects.


METHODS
The bulk bone of rabbit iliac crest was ground into micro-morselized bone, which was combined with BMP and type I collagen. The model of 1.5 cm bone defect was established in the middle shaft of the radius. Fifty-six rabbits were assigned to four repairing methods: autologous micro-morselized bone graft with BMP and type I collagen, autologous micro-morselized bone graft with type I collagen, autologous micro-morselized bone graft alone, and control group. The defect-repairing capability of each group was assessed by radiographic, histological, bone densitometry and biomechanical studies.


RESULTS
X-ray manifested that at the end of 8 weeks after operation, the bone defect treated with autologous micro-morselized bone graft with BMP and type I collagen was repaired completely, and at the end of 12 weeks after operation the bone defect treated with autologous micro-morselized bone and type I collagen was cured completely, but the bone defect treated with autologous micro-morselized alone was completely repaired. No healing was found in the control group. In the bone densitometry detection, the material with BMP exhibited the strongest defect-repairing capability in terms of amount increased and quality of the new bone at the end of 8 weeks and 12 weeks. The group with BMP has the best mechanical strength of all groups at the end of 12 weeks.


CONCLUSIONS
Autologous micro-morselized bone graft with BMP/type I collagen and autologous micro-morselized bone graft with type I collagen prove to be effective in repairing segmental bone defects. The autologous micro-morselized bone combined BMP and type I collagen is an excellent bone repairing material considering the satisfactory osteogenesis, osteo-conduction, and osteo-induction seen in this method.
Zou
Tianbiao
T
Department of Orthopaedics, Second Clinical College, Harbin Medical University, Harbin Heilongjiang, 150086, PR China.
Yan
Jinglong
J
Fu
Hailiang
H
Hou
Zhanjiang
Z
Wang
Shuhuai
S
Xia
Jingjun
J
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D024042:Collagen Type I / Q000737:chemistry*; D005260:Female; D008297:Male; D011817:Rabbits; D011884:Radius / Q000293:injuries / Q000503:physiopathology / Q000601:surgery*; D011897:Random Allocation; D014182:Transplantation, Autologous
D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D024042:Collagen Type I