Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly.
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
Calvo-Muñoz
M-L
ML
Apibio, Zone ASTEC, 15 rue des Martyrs, F-38054 Grenoble Cedex 9, France.
Dupont-Filliard
A
A
Billon
M
M
Guillerez
S
S
Bidan
G
G
Marquette
C
C
Blum
L
L
D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011108:Polymers; D011758:Pyrroles
D008567:Membranes, Artificial; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D008165:Luminol; C031912:6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione