[Effects of root-applied glycinebetaine on the composition and function of wheat thylakoid membrane under drought stress].
Two wheat cultivars (Triticum aestivum L.), HF9703 tolerant to drought and SN215953 sensitive to drought, were used to study the effects of glycinebetaine on the composition and function of thylakoid membrane. The wheat seedlings with two leaves were pretreated with Hoagland solution containing 1.5 mmol/L glycinebetaine (GB) for 72 h, then cultured with Hoagland solution containing 15% PEG-6000 for 48 h. The seedling leaves were used for mensuration. The results indicated that the chlorophyll, monogalactosyl diaylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and phosphatidylglycerol (PG) contents of the two wheat cultivars decreased significantly (P<0.05) under drought stress. GB alleviated their decrease. The sulfoquinovosyl diacylglycerol (SQDG) content, trans-hexadecenoic [16:1(3t)] and saturated fatty acid content of MGDG in HF9703 increased significantly (P<0.05), while in SN215953, the sulfoquinovosyl diacylglycerol (SQDG) and trans-hexadecenoic [16:1(3t)] contents decreased significantly under drought stress, but the saturated fatty acid content of MGDG increased slightly. These differences between the two wheat cultivars might account for much of the difference in drought tolerance between them. Ca2+-ATPase activity of thylakoid membrane, Hill-reaction activity and photosynthesis were decreased markedly (P<0.05) by drought stress. GB ameliorated these effects on thylakoid membrane, and the effect of GB on SN215953 was stronger than on HF9703. Discussion was made on the possible mechanism of the alleviating effect of root-applied GB on the composition and function of thylakoid membrane.
Zhao
Xin-Xi
XX
Department of Plant Science, Shandong Agricultural University, Tai'an 271018, China.
Ma
Qian-Quan
QQ
Yang
Xing-Hong
XH
Liang
Chao
C
Fang
Yan
Y
Wang
Wei
W
Zou
Qi
Q
D001622:Betaine / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D055864:Droughts; D038983:Galactolipids / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D020524:Thylakoids / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D038983:Galactolipids; D010715:Phosphatidylglycerols; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; D002734:Chlorophyll; D001622:Betaine; D000252:Calcium-Transporting ATPases