title: Crystallographic data collection using a 0.22% bandwidth multilayer.
abstract: To bridge the gap between traditional multilayer and crystal optics a high-resolution multilayer monochromator with a bandwidth of 0.22% has been designed and installed on a bending-magnet beamline (F3) at the Cornell High Energy Synchrotron Source (CHESS) to provide an unfocused monochromatic X-ray beam for protein crystallography experiments. Crystallographic data of excellent quality from a medium-sized protein, Concanavalin A, were collected and processed using standard crystallographic programs. The data were successfully used for a structure solution and refinement. The flux from the multilayer monochromator is enhanced, relative to that from a flat Si(111) monochromator, by a factor of 5; consequently, data collection is faster and/or smaller samples may be used. At the same time, the bandwidth is narrow enough to avoid streaked spots. This experiment suggests that multilayer optics may play a valuable role in satisfying the demands of the structural biology community for rapid X-ray data collection, particularly at under-utilized bending-magnet beamlines.
authors.affiliation: Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, NY 14853-8001, USA.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Englich
authors.forename: Alexander
authors.initials: A
authors.lastname: Kazimirov
authors.forename: Qun
authors.initials: Q
authors.lastname: Shen
authors.forename: Don H
authors.initials: DH
authors.lastname: Bilderback
authors.forename: Sol M
authors.initials: SM
authors.lastname: Gruner
authors.forename: Quan
authors.initials: Q
authors.lastname: Hao
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry*; D055095:Optics and Photonics; D011487:Protein Conformation; D017356:Synchrotrons
chemical_list: D046911:Macromolecular Substances; D003208:Concanavalin A