Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria.
When docosahexaenoic acid (DHA)-producing Moritella marina strain MP-1 was cultured in the medium containing 0.5 microg cerulenin ml-1, an inhibitor for fatty acid biosynthesis, the cells grew normally, but the content of DHA in the total fatty acids increased from 5.9-19.4%. The DHA yield of M. marina strain MP-1 cells also increased from 4 to 13.7 mg l-1 by cerulenin treatment. The same effect of cerulenin was observed in eicosapentaenoic acid (EPA)-producing Shewanella marinintestina strain IK-1 grown in the medium containing 7.5 microg cerulenin ml-1, and the cerulenin treatment increased the EPA yield from 1.6 to 8 mg l-1. The use of cerulenin is, therefore, advantageous to increase the content of intracellular polyunsaturated fatty acids (PUFA) in particular PUFA-containing phospholipids in bacterial cells.
Morita
Naoki
N
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, 062-8517, Japan. morita.n@aist.go.jp
Nishida
Takanori
T
Tanaka
Mika
M
Yano
Yutaka
Y
Okuyama
Hidetoshi
H
D018533:Biomass; D002569:Cerulenin / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods*; D034221:Moritella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D020592:Shewanella / Q000187:drug effects / Q000378:metabolism*; D014260:Triclosan / Q000494:pharmacology
D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D002569:Cerulenin; D014260:Triclosan