Proteomic and genomic characterization of chromatin complexes at a boundary.
We have dissected specialized assemblies on the Saccharomyces cerevisiae genome that help define and preserve the boundaries that separate silent and active chromatin. These assemblies contain characteristic stretches of DNA that flank particular regions of silent chromatin, as well as five distinctively modified histones and a set of protein complexes. The complexes consist of at least 15 chromatin-associated proteins, including DNA pol epsilon, the Isw2-Itc1 and Top2 chromatin remodeling proteins, the Sas3-Spt16 chromatin modifying complex, and Yta7, a bromodomain-containing AAA ATPase. We show that these complexes are important for the faithful maintenance of an established boundary, as disruption of the complexes results in specific, anomalous alterations of the silent and active epigenetic states.
Tackett
Alan J
AJ
Rockefeller University, New York, NY 10021, USA.
Dilworth
David J
DJ
Davey
Megan J
MJ
O'Donnell
Michael
M
Aitchison
John D
JD
Rout
Michael P
MP
Chait
Brian T
BT
D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D020868:Gene Silencing / Q000502:physiology*; D016681:Genome, Fungal; D023281:Genomics; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D029701:Saccharomyces cerevisiae Proteins