Direct interaction and cooperative role of tumor suppressor p16 with band 3 (AE1).
By using the C-terminal 112-residue of band 3 to screen the K562 cDNA library, we find that the p16 interacts with band 3, which was confirmed both in yeast and in mammalian cells. Functional experiments show that p16 facilitates the movement of band 3 to plasma membrane with increased anion transport activity in 293t cells. Moreover, expression of endogenous p16 in 293t cells was increased at 24 and 36 h after transfection with band 3. Our findings provide a novel regulation pathway for both band 3 and p16.
Fu
Guo-Hui
GH
Department of Pathophysiology, Shanghai Institute of Hematology, Rui-Jin Hospital, Shanghai Second Medical University (SSMU), Shanghai 200025, PR China. guohuifu@shsmu.edu.cn
Wang
Yong
Y
Xi
Yu-Hui
YH
Shen
Wei-Wei
WW
Pan
Xiao-Yan
XY
Shen
Wu-Zhong
WZ
Jiang
Xiao-Shu
XS
Chen
Guo-Qiang
GQ
D000595:Amino Acid Sequence; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D001457:Anion Exchange Protein 1, Erythrocyte; D019941:Cyclin-Dependent Kinase Inhibitor p16