Differentiation of Mycoplasma gallisepticum strains using amplified fragment length polymorphism and other DNA-based typing methods.
Amplified fragment length polymorphism (AFLP) was used to type 34 strains of Mycoplasma gallisepticum (MG) including vaccine strains ts-11, 6/85, and F. Using AFLP, a total of 10 groups, with 30 distinguishable AFLP typing profiles, were generated in the analysis. The AFLP method was able to identify and differentiate both MG field strains from recent outbreaks and those that were epidemiologically related. The AFLP procedure will provide assistance in identifying the sources of mycoplasma infections. Vaccine strains were also differentiated from other field strains, which will be useful in the evaluation of vaccination programs. The AFLP discrimination potential was compared to other molecular typing techniques such as gene-targeted typing by DNA sequence analysis of the MG cytadhesin-like protein encoding gene, mgc2, and random amplified polymorphic DNA assay on the same MG isolates. The three assays correlated well with one another, with AFLP analysis having a much higher discriminatory power and reproducibility.
Hong
Yang
Y
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-4875, USA.
García
Maricarmen
M
Levisohn
Sharon
S
Savelkoul
Paul
P
Leiting
Victoria
V
Lysnyansky
Inna
I
Ley
David H
DH
Kleven
Stanley H
SH
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D045686:Mycoplasma gallisepticum / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D001426:Bacterial Proteins; D017931:DNA Primers