title: 'Spiking' as a rapid method for differentiation of Candida albicans from other yeast species.
abstract: This paper describes a simple and rapid method for the differentiation of Candida albicans from other yeast species in primary cultures based on colonial morphology following incubation in carbon dioxide. The technique has superior sensitivity to the traditional germ-tube method and requires no additional laboratory tests. In a busy laboratory, this can result in significant savings in cost and time, as well as improvements in patient care.
authors.affiliation: Department of Medical Microbiology, University of Wales College of Medicine, Cardiff CF14 4XN, UK. barnesra@cf.ac.uk
authors.forename: R A
authors.initials: RA
authors.lastname: Barnes
authors.forename: L
authors.initials: L
authors.lastname: Vale
mesh_terms: D000362:Agar / Q000191:economics / Q000592:standards*; D002176:Candida albicans / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D002245:Carbon Dioxide / Q000191:economics / Q000592:standards*; D015169:Colony Count, Microbial; D017046:Cost Savings; D003470:Culture Media / Q000191:economics / Q000592:standards*; D004271:DNA, Fungal / Q000235:genetics; D003937:Diagnosis, Differential; D006358:Hot Temperature; D006801:Humans; D016533:Mycological Typing Techniques / Q000191:economics / Q000379:methods* / Q000592:standards; D010641:Phenotype; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D002245:Carbon Dioxide; D000362:Agar