Electrogeneration of a poly(pyrrole)-NTA chelator film for a reversible oriented immobilization of histidine-tagged proteins.
This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagged glucose oxidase led to the formation of a close-packed enzyme monolayer at the poly(pyrrole) surface, and the reversibility and reproducibility of this affinity process were demonstrated.
Haddour
Naoufel
N
Laboratoire d'Electrochimie Organique et de Photochimie Rédox (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
Cosnier
Serge
S
Gondran
Chantal
C
D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*
D002614:Chelating Agents; D011108:Polymers; D011758:Pyrroles; D001360:Avidin; C067635:polypyrrole; D010984:Platinum; D006639:Histidine; D001710:Biotin; D003300:Copper; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D009571:Nitrilotriacetic Acid