title: Identification of an imipenem-resistant Pseudomonas aeruginosa clone among patients in a hospital in Rio de Janeiro.
abstract: A total of 85 Pseudomonas aeruginosa isolates were obtained from October 1999 to April 2000 in a tertiary care hospital in Rio de Janeiro, Brazil. The imipenem susceptibility was evaluated by disk diffusion and agar dilution methods, and the clonal relationship among 67 isolates was examined by macrorestriction profile analysis following pulsed-field gel electrophoresis. Imipenem resistance was observed in 52 (61.2%) isolates. Imipenem-resistant P. aeruginosa isolates were separated into 10 genotypes, 73% of which belonged to genotype A. Identification of a single P. aeruginosa clone with a high rate of imipenem resistance emphasizes the need to control the transmission of this organism among patients.
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Kokis
authors.forename: B M
authors.initials: BM
authors.lastname: Moreira
authors.forename: F L P C
authors.initials: FL
authors.lastname: Pellegrino
authors.forename: M G
authors.initials: MG
authors.lastname: Silva
authors.forename: J B
authors.initials: JB
authors.lastname: Long
authors.forename: C C R
authors.initials: CC
authors.lastname: Bastos
authors.forename: K R N
authors.initials: KR
authors.lastname: Santos
mesh_terms: D001938:Brazil / Q000453:epidemiology; D015169:Colony Count, Microbial; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006301:Health Services Needs and Demand; D006772:Hospitals, Military; D006785:Hospitals, University; D006801:Humans; D015378:Imipenem; D005779:Immunodiffusion; D017053:Infection Control / Q000379:methods; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D015378:Imipenem