Laser desorption/ionization time-of-flight mass spectrometry of triacylglycerols in oils.
Selected triacylglycerols (TAGs) were directly analyzed on a standard stainless steel target plate by laser desorption/ionization time-of-flight mass spectrometry (LDI-TOFMS). Sodium and potassium ion adducts of TAGs were produced, and the thermal desorption/ionization mechanism was invoked to rationalize the experimental observations. The method permits a simple and fast qualitative analysis of TAGs. Advantages of this approach relative to matrix-assisted laser desorption/ionization (MALDI) are simpler sample preparation, lack of need to use a matrix with consequent absence of matrix interference peaks in the spectra, and potential improvements in shot-to-shot reproducibility due to the absence of the crystallization step resulting in a more homogenously deposited sample. The procedure was successfully applied to the determination of TAGs in whole oils, yielding very fast TAG fingerprints.
Calvano
Cosima D
CD
Università degli Studi di Bari, Dipartimento di Chimica, Italy.
Palmisano
Francesco
F
Zambonin
Carlo G
CG
D010938:Plant Oils / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry*
D010938:Plant Oils; D014280:Triglycerides