title: Electrogeneration of a poly(pyrrole)-NTA chelator film for a reversible oriented immobilization of histidine-tagged proteins.
abstract: This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagged glucose oxidase led to the formation of a close-packed enzyme monolayer at the poly(pyrrole) surface, and the reversibility and reproducibility of this affinity process were demonstrated.
authors.affiliation: Laboratoire d'Electrochimie Organique et de Photochimie Rédox (CNRS UMR 5630), Institut de Chimie Moléculaire de Grenoble FR CNRS 2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
authors.forename: Naoufel
authors.initials: N
authors.lastname: Haddour
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Chantal
authors.initials: C
authors.lastname: Gondran
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D011108:Polymers; D011758:Pyrroles; D001360:Avidin; C067635:polypyrrole; D010984:Platinum; D006639:Histidine; D001710:Biotin; D003300:Copper; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D009571:Nitrilotriacetic Acid