title: The synthesis of 7-substituted-3-dinitrostyryl cephalosporins and their ability for detecting extended spectrum beta-lactamases (ESBLs).
abstract: We synthesized 7-substituted-3-(2,4-dinitrostyryl)cephalosporin derivatives which were Nitrocefin analogs, for detecting extended spectrum beta-lactamase (ESBL) specifically. HMRZ-86 which has carboxypropyloxyimino group on 7-aminothiazolacetamide substituent were not hydrolyzed by class A, C and D beta-lactamases, but it was hydrolyzed by ESBL and metallo beta-lactamase (class B), then its color changed from yellow to red. The hydrolysis of metallo beta-lactamase was inhibited by adding sodium mercapto acetic acid (SMA). Therefore HMRZ-86 is a useful chromogenic agent to detect ESBL specifically.
authors.affiliation: Research Center for Anti-infective Drugs, Kitasato Institure, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hanaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Harada
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kubo
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Atsuda
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sunakawa
mesh_terms: D002511:Cephalosporins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D003116:Color; D006868:Hydrolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D013329:Structure-Activity Relationship; D001618:beta-Lactamases / Q000187:drug effects*
chemical_list: D002511:Cephalosporins; C487639:HMRZ 86; D001618:beta-Lactamases