title: [High-performance liquid chromatography combined with mass spectrum analysis for chromatographic fingerprinting of compound dandelion enema].
abstract: OBJECTIVE
  To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases.
  
  
  METHODS
  Three-dimensional high-performance liquid chromatography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting.
  
  
  RESULTS
  By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde.
  
  
  CONCLUSIONS
  HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
authors.affiliation: Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yan
authors.forename: Shi-Ting
authors.initials: ST
authors.lastname: Liu
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Xu
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Luo
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Tan
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000285:Administration, Rectal; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D031205:Taraxacum / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal