title: Tonic mechanosensitivity of outer hair cells after loss of tip links.
abstract: Tip links - the extracellular connectors between the distal ends of adjacent stereocilia - are essential for the fast mechanical gating of hair-cell transducer channels. Transduction in the absence of tip links was investigated for outer hair cells of the adult guinea-pig cochlea by patch-clamp recordings of the whole-cell current during mechanical stimulation of the hair bundle. Loss of tip links induced by application of BAPTA led to permanently opened transducer channels, as evidenced by a constant inward current, loss of response to sinusoidal mechanical deflection of the hair bundle and block by the open-channel blocker dihydrostreptomycin (100 microM). Step deflection of the hair bundle (200-500 nm) in the inhibitory direction exponentially reduced this current to a constant value with time constant, tau(on), of the order of seconds. The current returned exponentially to the pre-stimulus level with time-constant, tau(off), also of the order of seconds. tau(on) was dependent on the inter-stimulus interval, Deltat, such that reducing this interval below about 40 s resulted in an exponentially faster response. tau(off) was independent of Deltat. Application of the calcium ionophore, ionomycin (10 microM), showed that tau(on) became independent of Deltat after saturating elevation of the intracellular Ca(2+) concentration. Flash-photolytic release of intracellular caged calcium (25-microM NP-EGTA/AM) showed that tau(on) is dependent on intracellular Ca(2+) concentration. These experiments imply an intracellular, calcium-dependent gating mechanism for hair-cell transducer channels.
authors.affiliation: Department of Otolaryngology, Section of Physiological Acoustics and Communication, University of Tübingen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Meyer
authors.forename: Serena
authors.initials: S
authors.lastname: Preyer
authors.forename: Susanne I
authors.initials: SI
authors.lastname: Hofmann
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Gummer
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004096:Dihydrostreptomycin Sulfate / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000502:physiology*; D007425:Intracellular Membranes / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008465:Mechanoreceptors / Q000187:drug effects* / Q000502:physiology*; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D010812:Physical Stimulation; D013997:Time Factors
chemical_list: D007476:Ionophores; D004533:Egtazic Acid; D015759:Ionomycin; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium; D004096:Dihydrostreptomycin Sulfate