Versatile protein microarray based on carbohydrate-binding modules.
Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical approach. These include the prerequisite for numerous proteins in active and purified form, ineffectual immobilization strategies and inadequate means for quality control of the considerable numbers of multiple reagents. This study describes a simple yet efficient strategy for the production of non-DNA microarrays, based on the tenacious affinity of a carbohydrate-binding module (CBM) for its three-dimensional substrate, i.e., cellulose. Various microarray formats are described, e.g., conventional and single-chain antibody microarrays and peptide microarrays for serodiagnosis of human immunodeficiency virus patients. CBM-based microarray technology overcomes many of the previous obstacles that have hindered fabrication of non-DNA microarrays and provides a technically simple but effective alternative to conventional microarray technology.
Ofir
Keren
K
Zephyr ProteomiX, Kiryat-Shmona, Israel.
Berdichevsky
Yevgeny
Y
Benhar
Itai
I
Azriel-Rosenfeld
Ronit
R
Lamed
Raphael
R
Barak
Yoav
Y
Bayer
Edward A
EA
Morag
Ely
E
D015492:AIDS Serodiagnosis / Q000379:methods; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D006678:HIV; D006801:Humans; D040081:Protein Array Analysis; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*
D002241:Carbohydrates; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins