title: End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe.
abstract: The rod-shaped Schizosaccharomyces pombe cell grows in a polarized fashion from opposing ends. Correct positioning of the growth zones is directed by the polarity marker Tea1 located at the cell ends where actin patches accumulate and cell growth takes place. We show that the S. pombe homologue of Saccharomyces cerevisiae SLA2, a protein involved in cortical actin organization and endocytosis, provides a link between the polarity marker and the growth machinery. In wild-type fission yeast cells, this homologue End4/Sla2 is enriched at cell ends during interphase and localizes to a medial ring at cell division, mirroring the actin localization pattern throughout the cell cycle. Proper localization relies on membrane trafficking and is independent of both the actin and microtubule cytoskeletons. End4/Sla2 is required for the establishment of new polarised growth zones, and deletion of its C-terminal talin-like domain prevents the establishment of a new growth zone after cell fission. We propose that End4/Sla2 acts downstream of the polarity marker Tea1 and is implicated in the recruitment of the actin cytoskeleton to bring about polarised cell growth.
authors.affiliation: Cell Cycle Lab Cancer Research UK, 44 Lincoln's Inn Field, London, WC2A 3PX, UK. s.castagnetti@cancer.org.uk
authors.forename: Stefania
authors.initials: S
authors.lastname: Castagnetti
authors.forename: Ralf
authors.initials: R
authors.lastname: Behrens
authors.forename: Paul
authors.initials: P
authors.lastname: Nurse
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology; D020126:Brefeldin A / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins; D004705:Endocytosis; D005656:Fungal Proteins / Q000502:physiology; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000502:physiology*; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D012568:Schizosaccharomyces / Q000378:metabolism / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000096:biosynthesis* / Q000502:physiology*; D013696:Temperature; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines
chemical_list: D000199:Actins; D000935:Antifungal Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; C082266:SLA2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C432671:Tea1 protein, S pombe; D013844:Thiazoles; D053778:Thiazolidines; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; C037067:latrunculin A