title: Prepubertal unilateral spermatic vessel ligation decreases haploid cell population of ipsilateral testis postpubertally in rats.
abstract: DNA flow cytometry is a sensitive and rapid technique to evaluate the germ cell maturation of testis. The haploid cell population of testis specifically denotes testicular maturation. High ligation of spermatic vessels (HLSV) and low ligation of spermatic vessels (LLSV) have been described in the surgical treatment of high undescended testis. Estimation of germ cell population of testis after these maneuvers has not been performed so far by DNA flow cytometry; hence this study was designed. Thirty male rats, aged 30 days, were randomized into three groups. Group I underwent sham surgery; group II underwent unilateral HLSV-ligation and division of main spermatic vessels, similar to the Fowler-Stephens technique; and group III underwent unilateral LLSV-ligation and division of main spermatic vessels close to the testis, similar to the Koff and Sethi technique. Thirty days later, the ipsilateral testes were harvested, and haploid, diploid, and tetraploid cells were counted by DNA flow cytometry. The mean (+/-SD) percentage of the haploid cell population was 68.7+/-4.8, 49.3+/-6.5, and 50.8+/-6.4 in groups I, II, and III, respectively. There was a significant (p<0.05) decrease in haploid cell population between groups I and II and between groups I and III. However, there was no significant difference between groups II and III. In conclusion, prepubertal HLSV as well as LLSV decreases the haploid cell population of ipsilateral testis in rats. LLSV has no advantage over HLSV.
authors.affiliation: Children's Research Center, Our Lady's Hospital for Sick Children, Crumlin, Dublin, 12, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Srinivas
authors.forename: Barry
authors.initials: B
authors.lastname: Kilmartin
authors.forename: S N
authors.initials: SN
authors.lastname: Das
authors.forename: Prem
authors.initials: P
authors.lastname: Puri
mesh_terms: D000818:Animals; D003456:Cryptorchidism / Q000601:surgery; D005434:Flow Cytometry; D006238:Haploidy; D008026:Ligation; D008297:Male; D008833:Microcirculation / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013085:Spermatic Cord / Q000098:blood supply / Q000601:surgery*; D013737:Testis / Q000098:blood supply / Q000166:cytology* / Q000503:physiopathology