Screening method of agents against the R factor by the use of an Hfr made by integrative suppression with an R factor.
By the use of an Hfr strain made by integrative suppression of a derepressed R factor, R100-1 in a dnaA-ts mutant of Escherichia coli and several other appropriate control strains with and without the R factor, a screening system was established which can be used to detect agents which are either inhibitory to the replication of the R factor and lead to its "curing" or are preferentially inhibitory to the growth of the R-carrying bacteria. The validity of this system was shown with several agents already known to be experimentally effective.
Yoshikawa
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D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D008828:Microbiological Techniques; D009154:Mutation; D011815:R Factors / Q000235:genetics*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D000362:Agar