title: Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia.
abstract: Seed dormancy in Nicotiana plumbaginifolia is characterized by an abscisic acid accumulation linked to a pronounced germination delay. Dormancy can be released by 1 year after-ripening treatment. Using a cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach we compared the gene expression patterns of dormant and after-ripened seeds, air-dry or during one day imbibition and analyzed 15,000 cDNA fragments. Among them 1020 were found to be differentially regulated by dormancy. Of 412 sequenced cDNA fragments, 83 were assigned to a known function by search similarities to public databases. The functional categories of the identified dormancy maintenance and breaking responsive genes, give evidence that after-ripening turns in the air-dry seed to a new developmental program that modulates, at the RNA level, components of translational control, signaling networks, transcriptional control and regulated proteolysis.
authors.affiliation: UMR 204 de Biologie des Semences, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, route de Saint Cyr, 78026 Versailles Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bove
authors.forename: Philippe
authors.initials: P
authors.lastname: Lucas
authors.forename: Béatrice
authors.initials: B
authors.lastname: Godin
authors.forename: Laurent
authors.initials: L
authors.lastname: Ogé
authors.forename: Marc
authors.initials: M
authors.lastname: Jullien
authors.forename: Philippe
authors.initials: P
authors.lastname: Grappin
mesh_terms: D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D014176:Protein Biosynthesis / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D018076:DNA, Complementary