title: Force microscopy studies of fibronectin adsorption and subsequent cellular adhesion to substrates with well-defined surface chemistries.
abstract: Molecular force spectroscopy was used to study the mechanical behavior of plasma fibronectin (FN) on mica, gold, poly(ethylene glycol), and -CH(3), -OH, and -COOH terminated alkanethiol self-assembled monolayers. Proteins were examined at two concentrations, one resulting in a saturated surface with multiple intermolecular interactions referred to as the aggregate state and another resulting in a semiaggregate state where the proteins were neither completely isolated nor completely aggregated. Modeling of the force-extension data using two different theories resulted in similar trends for the fitted thermodynamic parameters from which insight into the protein's binding state could be obtained. Aggregated proteins adsorbed on hydrophobic surfaces adopted more rigid conformations apparently as a result of increased surface denaturation and tighter binding while looser conformations were observed on more hydrophilic surfaces. Studies of FN in a semiaggregate state showed heterogeneity in the model's thermodynamic parameters suggesting that, in the early stages of nonspecific adsorption, multiple protein conformations exist, each having bound irreversibly to the substrate. Proteins in this state all demonstrated a more rigid conformation than in the corresponding aggregate studies due to the greater number of substrate contacts available to the protein. Finally, the force spectroscopy experiments were examined for any biocompatibility correlation by seeding substrates with human umbilical vascular endothelial cells. As predicted from the models used in this work, surfaces with aggregated FN promoted cellular deposition while surfaces with FN in a semiaggregate state appeared to hinder cellular deposition and growth. The atomic force microscope's use as a means for projecting surface biocompatibility, although requiring additional testing, does look promising.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Pamela Y
authors.initials: PY
authors.lastname: Meadows
authors.forename: Gilbert C
authors.initials: GC
authors.lastname: Walker
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology; D005353:Fibronectins / Q000737:chemistry* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry*; D011487:Protein Conformation; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000538:Aluminum Silicates; D001672:Biocompatible Materials; D005353:Fibronectins; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold; C011934:mica