Eicosanoids influence in vitro elongation of plasmatocytes from the tobacco hornworm, Manduca sexta.
Nodule formation is the predominant insect cellular defense reaction to bacterial challenges, responsible for clearing the largest proportion of infecting bacteria from hemolymph circulation. Hemocyte spreading behavior is a critical step in the nodulation process. It has been suggested that eicosanoids mediate several steps in the process. However, the influence of eicosanoids on hemocyte spreading has not been investigated in detail. To test the hypothesis that eicosanoids mediate hemocyte spreading behavior, I treated larvae of the tobacco hornworm, Manduca sexta, with eicosanoid biosynthesis inhibitors and later assessed plasmatocyte elongation on glass slides. Plasmatocytes from larvae treated with dexamethasone did not elongate to the extent of plasmatocytes from untreated control larvae. The dexamethasone effect on plasmatocyte elongation was expressed in a dose-dependent manner and was reversed by injecting dexamethasone-treated larvae with the eicosanoid-precursor fatty acid, arachidonic acid. Palmitic acid, which is not substrate for eicosanoid biosynthesis, did not reverse the influence of dexamethasone on plasmatocyte elongation. Finally, plasmatocytes from larvae treated with a range of eicosanoid biosynthesis inhibitors did not elongate to the extent of plasmatocytes from control larvae. Plasmatocyte width did not appear to be influenced in this study. These findings strongly support the idea that insect plasmatocyte elongation is influenced by eicosanoids.
Miller
Jon S
JS
Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA. jsmiller@niu.edu
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015777:Eicosanoids / Q000378:metabolism*; D006458:Hemolymph / Q000166:cytology; D007814:Larva / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016014:Linear Models; D018402:Manduca / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D019308:Palmitic Acid / Q000494:pharmacology; D013997:Time Factors
D015777:Eicosanoids; D016718:Arachidonic Acid; D019308:Palmitic Acid; D003907:Dexamethasone