[Plaferon LB in prevention of radiation-induced changes in lipid metabolism indices and erythrocyte deformability rate].
Oxidative damage of biological membranes plays critical role in the process of radiation-induced damages in tissues and cells. It involves reactive oxygen species generated under ionizing radiation. Development of radiation-induced oxidative stress facilitates the intensification of lipid peroxidation and fatty acid reorganization, which, in its turn, results in decreased lipid matrix fluidity, changes in biophysical properties of membranes, increased rigidity of membrane lipid-protein surface. Radiation-induced damage of cellular and subcellular membrane structures results in cellular metabolism disorder, which facilitates farther dysfunction of tissues and changes in homeostasis of whole organism. Hence, the search for effective protection is of actual interest of contemporary radiology. Our study aimed to determine membrane protective properties of Plaferon LB under radiation. On the basis of obtained results we can conclude that intensification of free radical oxidation, accompanying radiation damage, facilitates the activation of lipolysis in a body and functional and structural disorders of erythrocytes revealed by lowered deformability of erythrocytes and accumulation of methaemoglobin. Both vitamin C and Plaferon LB were found ineffective for correction of lipid metabolism at early stage of post radiation damage; although facilitating a decrease in oxidation intensity in blood, they provided retention of erythrocyte deformability and thereby, partial retention of rheological properties of blood. It should be mentioned that in contrast with vitamin C, Plaferon LB, apart from its ability to restrict reactive oxygen formation, revealed ability to stabilize erythrocyte membranes and thereby to prevent hemolysis.
Saralidze
M A
MA
Kasrashvili
M V
MV
Tkhilava
N G
NG
Pavliashvili
A T
AT
Bakhutashvili
V I
VI
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use; D004907:Erythrocyte Deformability; D005609:Free Radicals; D006706:Homeostasis; D050356:Lipid Metabolism; D009479:Neuropeptides / Q000008:administration & dosage / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D017382:Reactive Oxygen Species
D005609:Free Radicals; D009479:Neuropeptides; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C091912:plaferon; D001205:Ascorbic Acid