title: Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution.
abstract: For experimental machine perfusion (MP) of the liver, the modified University of Wisconsin solution (UW-G) is most often used. In our search for an enriched MP preservation solution, Polysol was developed. Polysol is enriched with various amino acids, vitamins, and other nutrients for the liver metabolism. The aim of this study was to compare Polysol with UW-G for MP preservation of the liver. Rat livers were preserved during 24 hours with hypothermic MP using UW-G (n = 5) or Polysol (n = 5). Hepatocellular damage (aspartate aminotransferase [AST], alanine aminotransferase [ALT], lactate dehydrogenase [LDH], alpha-glutathione-S-transferase [alpha-GST]) and bile production were measured during 60 minutes of reperfusion (37 degrees C) with Krebs-Henseleit buffer. Control livers were reperfused after 24 hours of cold storage in UW (n = 5). MP using UW-G or Polysol showed less liver damage when compared with controls. Livers machine perfused with Polysol showed less enzyme release when compared to UW-G. Bile production was higher after MP using either UW-G or Polysol compared with controls. In conclusion, machine perfusion using Polysol results in better quality liver preservation than cold storage with UW and machine perfusion using UW-G.
authors.affiliation: Surgical Laboratory, Department of Surgery, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Maud
authors.initials: M
authors.lastname: Bessems
authors.forename: Benedict M
authors.initials: BM
authors.lastname: Doorschodt
authors.forename: Arlène K
authors.initials: AK
authors.lastname: van Vliet
authors.forename: Thomas M
authors.initials: TM
authors.lastname: van Gulik
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D005978:Glutathione / Q000494:pharmacology; D007036:Hypothermia, Induced; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000473:pathology / Q000502:physiology; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D009929:Organ Size; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; C504758:polysol solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose