title: Experimental and bioinformatic approaches for interrogating protein-protein interactions to determine protein function.
abstract: An ambitious goal of proteomics is to elucidate the structure, interactions and functions of all proteins within cells and organisms. One strategy to determine protein function is to identify the protein-protein interactions. The increasing use of high-throughput and large-scale bioinformatics-based studies has generated a massive amount of data stored in a number of different databases. A challenge for bioinformatics is to explore this disparate data and to uncover biologically relevant interactions and pathways. In parallel, there is clearly a need for the development of approaches that can predict novel protein-protein interaction networks in silico. Here, we present an overview of different experimental and bioinformatic methods to elucidate protein-protein interactions.
authors.affiliation: Health and Environment Unit, Faculty of Medicine, Eastern Quebec Proteomics Centre, 2705 Laurier Blvd, Ste-Foy, Quebec, Canada G1 V 4 G2.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Droit
authors.forename: Guy G
authors.initials: GG
authors.lastname: Poirier
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Hunter
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D016208:Databases, Factual; D006801:Humans; D020407:Internet; D013058:Mass Spectrometry / Q000379:methods; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics
chemical_list: D011506:Proteins