title: [Experimental study of human skin fibroblasts cultured in three-dimension(3D)].
abstract: OBJECTIVE
  To investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL).
  
  
  METHODS
  The human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology.
  
  
  RESULTS
  The flow cytometry showed that the G0/G1, stage cells were 79% +/- 3%, 87% +/- 2% after the 7 days and 14 days separately, and there were not apoptosis peak observed. RT-PCR analysis revealed that the mRNA expression of TGF beta1, and fibronectin had no difference between human skin fibroblasts cultured in 3D and 2D. Electron microscope showed the cells were plenty of chromatin and organelles.
  
  
  CONCLUSIONS
  The proliferation of the human skin fibroblasts in FPCL is slow, but its biological viability is better.
authors.affiliation: Center for Special Medicine, Hospital of PLA, Beijing, China.
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Jing-ning
authors.initials: JN
authors.lastname: Huan
authors.forename: Yu-lin
authors.initials: YL
authors.lastname: Chen
authors.forename: Sheng-de
authors.initials: SD
authors.lastname: Ge
authors.forename: Zhi-yang
authors.initials: ZY
authors.lastname: Fang
authors.forename: Tian-xiang
authors.initials: TX
authors.lastname: Ouyang
authors.forename: Xin
authors.initials: X
authors.lastname: Xing
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D051381:Rats; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003094:Collagen