title: Lung edema formation during cold perfusion: important differences between rat and porcine lung.
abstract: BACKGROUND
  The aim of this study was to investigate the effect of different perfusion pressures on edema formation during cold flush perfusion with the 2 most commonly used preservation solutions in clinical lung transplantation: Euro-Collins and Perfadex solutions.
  
  
  METHODS
  Isolated rat and porcine lungs were perfused for 3 minutes at 4 degrees C to 8 degrees C at a pressure of either 10, 15 or 20 mm Hg. Weight gain was recorded continuously. Weight gain per minute was calculated after the first phase of rapid weight gain was completed.
  
  
  RESULTS
  In the rat model, perfusion pressure of 10 mm Hg resulted in a macro- and microscopically apparent edema, irrespective of the type of preservation solution. Perfusion pressures of 10, 15 and 20 mm Hg have weight gains of 100%, 150% and 350%, respectively, after 3 minutes of perfusion. The corresponding weight gain per minute was 18%, 31% and 84% of the initial weight. There were no statistically significant differences in weight gain between the different solutions at equal perfusion pressure. In the porcine model the flow was extremely low at 10 mm Hg and no weight gain was registered, whereas the weight gain per minute at 15 and 20 mm Hg was 1.0% and 2.1% of the initial weight.
  
  
  CONCLUSIONS
  In porcine lungs, cold perfusion at 20 mm Hg gives minimal edema formulation, whereas in rat lungs the edema formation is deleterious, irrespective of the solution used.
authors.affiliation: Department of Cardiothoracic Surgery, Skejby Hospital, Aarhus University Hospital, Denmark.
authors.forename: Per
authors.initials: P
authors.lastname: Wierup
authors.forename: Quiming
authors.initials: Q
authors.lastname: Liao
authors.forename: Ramunas
authors.initials: R
authors.lastname: Bolys
authors.forename: Trygve
authors.initials: T
authors.lastname: Sjöberg
authors.forename: Bengt
authors.initials: B
authors.lastname: Rippe
authors.forename: Stig
authors.initials: S
authors.lastname: Steen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007036:Hypothermia, Induced / Q000009:adverse effects* / Q000379:methods; D008168:Lung / Q000098:blood supply / Q000473:pathology; D016040:Lung Transplantation / Q000473:pathology; D008297:Male; D009929:Organ Size; D010477:Perfusion; D011312:Pressure; D011652:Pulmonary Circulation / Q000502:physiology; D011654:Pulmonary Edema / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods