title: [Analysis of the sequences of the ribosomal DNA ITS2 region for differentiating anopheline mosquitoes within hyrcanus group].
abstract: OBJECTIVE
  To analyze the genetic characteristics of ribosomal DNA ITS2 region for differentiating anopheline mosquitoes within Hyrcanus group.
  
  
  METHODS
  The ribosomal DNA ITS2 region of both laboratory line and filed collected An. anthropophagus and An. sinensis as well as the field collected An. yatsushiroensis and An. lesteri were amplified and sequenced. The sequencing data were then analyzed for the restriction mapping using Omega Sequencing analysis program.
  
  
  CONCLUSIONS
  The length of the sequences of An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri are 472, 452, 456 and 456 bp respectively. The restriction mapping showed that there were different restriction digesting sites among the ribosomal DNA ITS2 region sequences from An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri. On the basis of the sequence differences among the anopheline species within Hyrcanus group, it is possible to develop new technique for genetic identification of anopheline mosquitoes.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.forename: Hua-yun
authors.initials: HY
authors.lastname: Zhou
authors.forename: Ju-lin
authors.initials: JL
authors.lastname: Li
authors.forename: Feng-hua
authors.initials: FH
authors.lastname: Li
authors.forename: Tong Gyu
authors.initials: TG
authors.lastname: Choe
authors.forename: Sung Chan
authors.initials: SC
authors.lastname: Yom
authors.forename: Guo-ding
authors.initials: GD
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D021903:DNA, Ribosomal Spacer