Use of surface-enhanced laser desorption ionization-time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila.
The 70-kDa heat shock protein (Hsp) family in all Drosophila species includes 2 environmentally inducible family members, Hsp70 and Hsp68. Two-dimensional gel electrophoresis revealed an unusual pattern of heat shock-inducible proteins in the species of the virilis group. Trypsin fingerprinting and microsequencing of tryptic peptides using ProteinChip Array technology identified the major isoelectric variants of Hsp70 family, including Hsp68 isoforms that differ in both molecular mass and isoelectric point from those in Drosophila melanogaster. The peculiar electrophoretic mobility is consistent with the deduced amino acid sequence of corresponding hsp genes from the species of the virilis group.
Zatsepina
Olga G
OG
Engelhardt Institute of Molecular Biology, Moscow, Russia.
Karavanov
Alexander A
AA
Garbuz
David G
DG
Shilova
Victoria
V
Tornatore
Peter
P
Evgen'ev
Michael B
MB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D004330:Drosophila / Q000145:classification* / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D018869:Heat-Shock Response / Q000235:genetics; D006358:Hot Temperature; D015151:Immunoblotting; D008970:Molecular Weight; D010449:Peptide Mapping; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018840:HSP70 Heat-Shock Proteins; D020033:Protein Isoforms; D014357:Trypsin