Expression of retinoid X receptor transcripts and their significance for developmental competence in in vitro-produced pre-implantation-stage bovine embryos.
Retinoid X receptors are frequently implicated in their role in embryonic development. However, there is no report regarding their expression in embryos with different qualities. In this study, expression pattern and levels of retinoid X receptor transcripts in different qualities and stages of in vitro-produced pre-implantation bovine embryos were examined using real-time polymerase chain reaction (PCR) techniques. Four independent cDNA pools per sample were prepared from mRNAs isolated from different developmental-stage embryos, after classifying them to be of good and bad quality. Primers were also designed to amplify products with PCR. Based on analysis, except retinoid X receptor gamma (RXRG) transcripts, the remaining two types (alpha and beta forms) were expressed in all samples with significantly higher (p < or = 0.05) levels until the eight-cell stage compared with the stages thereafter. However, the transcript levels for bad-quality embryos were consistently lower in all cases in comparison with good-quality embryos. RXRG was identified in 39% of the samples, but all in the pre-embryonic genome activation development stage. Therefore, strong expression patterns of these transcripts in earlier stages indicates their possible role during the maternal phase of embryo development, and the variations of copy number in embryos with different qualities substantiates their possible candidacy as potential quality markers. Moreover, identifying the transcript variations in different qualities and expression of RXRG at these stages is a novel input to the pre-implantation-stage knowledge.
Mamo
S
S
Institute of Animal Breeding Sciences, University of Bonn, Bonn, Germany. lilykacha2005@yahoo.com
Ponsuksili
S
S
Wimmers
K
K
Gilles
M
M
Schellander
K
K
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle / Q000196:embryology*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D047108:Embryonic Development; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D047490:Retinoid X Receptor alpha / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D049230:Retinoid X Receptor beta / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D047491:Retinoid X Receptor gamma / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D047488:Retinoid X Receptors / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D047490:Retinoid X Receptor alpha; D049230:Retinoid X Receptor beta; D047491:Retinoid X Receptor gamma; D047488:Retinoid X Receptors; D014157:Transcription Factors