title: Micro/Nanofluidic device for single-cell-based assay.
abstract: In this paper we have proposed and demonstrated a microfluidic device for a fast and parallel single-cell based assay. The proposed device is designed to passively capture single-cells or beads on multiple cell-positioning sites by a pre-defined fluidic stream and inject specific reagents or drugs onto each isolated single-cell. The device consists of surface-modified silicon channels capped with a grooved polydimethylsiloxane (PDMS) cover layer. A cell capture experiment has been performed using polystyrene beads as well as CHO DG44 living cells, and successful positioning of a single-cell on the cell-positioning sites was achieved. Also, we have demonstrated independent drug injection into a specific target cell.
authors.affiliation: Department of Electrical Engineering and Computer Science (Division of Electrical Engineering), Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea. ksyun@iml.kaist.ac.kr
authors.forename: Kwang-Seok
authors.initials: KS
authors.lastname: Yun
authors.forename: Euisik
authors.initials: E
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods