[Changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae Rhamnoides L].
OBJECTIVE
Hippophae rhamnoides L. possesses functions of antioxidation and radioprotection. This study was designed to investigate changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae rhamnoides L. (FHR) with cDNA microarray, and to explore possible mechanism of signal transduction on apoptosis.


METHODS
Total RNA was extracted from Bcap-37 cells before and after treatment of FHR. Two cDNA probes, labeled by Cy3-dUTP or Cy5-dUTP fluorescent dyes, were synthesized via reverse transcription, and hybridized with a microarray contained 13 824 human 14K cDNA. Differential gene expression profiles of FHR group and control group were analyzed by Genespring software.


RESULTS
After treatment of FHR, 305 genes were up-regulated, and 361 were down-regulated; 32 apoptosis-related genes were differentially expressed, and accounted for 0.23% of the total genes in cDNA microarray. Of the 32 apoptosis-related genes, 25 were up-regulated (average Ratio: 3.071), and 7 were down-regulated (average Ratio: 0.418). Bioinformatic analyses showed that the 32 genes, including CTNNB1, TSSC3, IGFBP4, IGFBP6, GADD34, TNFRSF10B, Caspase-9, and PCNA, related with apoptosis of Bcap-37 cells when treated with FHR.


CONCLUSIONS
Apoptosis of Bcap-37 cells induced by FHR relates with various genes through co-regulating of intracellular and extracellular signal transduction pathways.
Zhang
Ping
P
School of Life Sciences, East China Normal University, Shanghai, 200062, P. R. China.
Mao
Yu-Chang
YC
Sun
Bin
B
Qian
Min
M
Qu
Wei-Jing
WJ
D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D031281:Hippophae / Q000737:chemistry; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010946:Plants, Medicinal / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D015398:Signal Transduction
D005419:Flavonoids