Effect of lycium barbarum polysaccharide on human hepatoma QGY7703 cells: inhibition of proliferation and induction of apoptosis.
Lycium barbarum polysaccharide (LBP), extracted from Lycium barbarum that is a kind of traditional Chinese herb, is found to have anticancer activity. In this study, the effect of LBP on the proliferation rate, cell cycle distribution and apoptosis in the human hepatoma QGY7703 cell line were investigated. The effects of this compound were also tested on the concentration of calcium in cells. LBP treatment caused inhibition of QGY7703 cell growth with cycle arrest in S phase and apoptosis induction. The amount of RNA in cells and the concentration of intracellular Ca2+ were increased. Moreover, the distribution of calcium in cells was changed. Taken together, the study suggests that the induction of cell cycle arrest and the increase of intracellular calcium in apoptotic system may participate in the antiproliferative activity of LBP in QGY7703 cells.
Zhang
Min
M
Department of Food Science and Bioengineering, Tianjin University of Science and Technology, Tianjin 300222, PR China. zm0102@sina.com
Chen
Haixia
H
Huang
Jin
J
Li
Zhong
Z
Zhu
Caiping
C
Zhang
Shenghua
S
D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D032305:Lycium / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use; D012313:RNA / Q000032:analysis
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C500223:lycium barbarum polysaccharide; D012313:RNA; D002118:Calcium