Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I.
We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtgammaCAL1 and AtgammaCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtgammaCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtgammaCA and AtgammaCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtgammaCAs. All plant species analyzed contain both AtgammaCA and AtgammaCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtgammaCAL sequences show a deviation of functionally important active site residues with respect to gammaCAs but could form active interfaces in the interaction with AtgammaCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.
Perales
Mariano
M
Instituto de Investigaciones Biotecnológicas, IIB-INTECH (CONICET/UNSAM), C.C. 164, 7130 Chascomús, Argentina.
Parisi
Gustavo
G
Fornasari
María Silvina
MS
Colaneri
Alejandro
A
Villarreal
Fernando
F
González-Schain
Nahuel
N
Echave
Julián
J
Gómez-Casati
Diego
D
Braun
Hans-Peter
HP
Araya
Alejandro
A
Zabaleta
Eduardo
E
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D019281:Dimerization; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009842:Oligopeptides / Q000235:genetics; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D029681:Arabidopsis Proteins; D007527:Isoenzymes; D024101:Mitochondrial Proteins; D009842:Oligopeptides; D002256:Carbonic Anhydrases; D042967:Electron Transport Complex I