[Study on RP-HPLC fingerprint of compound hongcao injection].
OBJECTIVE
To establish the fingerprint detecting standard of Compound Hongcao Injection by reversed phase high performance liquid chromatography.


METHODS
The chromatographic conditions were as follows: an Inersil-ODS-3 column was used; the mobile phases (acetontrile and 0.1% phosphoric acid) with gradient elution; the flow rate 1.0 mL x min(-1) and the UV absorbance detection at 300 nm.


RESULTS
Under the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation according to the technical requirements of fingerprint on Injection of Chinese traditional medicine.


CONCLUSIONS
17 common peaks as well as their retention times and peak area ratios on HPLC fingerprint can be used as the important parameters of the quality control for Compound Hongcao Injection.
Lan
Yan-Yu
YY
School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
Liu
Li-Na
LN
Wang
Yong-Lin
YL
Li
Yong-Jun
YJ
Huang
Yong
Y
He
Xun
X
Zheng
Lin
L
Wang
Ai-Min
AM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011786:Quality Control
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal