Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato.
Eighteen clones representing copies of four Kunitz-type proteinase inhibitor group B genes (PKPI-B) obtained by polymerase chain reaction cloning of potato (Solanum tuberosum L. cv. Istrinskii) genomic DNA were sequenced and analyzed. Three new genes were found and named PKPI-B1, PKPI-B2, and PKPI-B10: these were represented by five, two, and seven clones, respectively. The remaining four clones corresponded to the formerly characterized PKPI-B9 gene. These data show that at least four PKPI-B encoding genes are harbored in the genome of potato cv. Istrinskii. Their analysis suggests that variability of PKPI-B encoding genes in potato is limited and could be explained by cross-hybridization events in the ancestor forms rather than by random mutagenesis.
Speranskaya
A S
AS
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Russia. annatar@aha.ru
Krinitsina
A A
AA
Poltronieri
P
P
Fasano
P
P
Santino
A
A
Shevelev
A B
AB
Valueva
T A
TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011480:Protease Inhibitors / Q000378:metabolism; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000235:genetics*
C053865:Kunitz-type protease inhibitor, plant; D010455:Peptides; D010940:Plant Proteins; D011480:Protease Inhibitors