title: 3'-immobilized probes with 2'-caps: synthesis of oligonucleotides with 2'-N-methyl-2'-(anthraquinone carboxamido)uridine residues.
abstract: [reaction: see text] A synthesis for oligodeoxynucleotides with a 3'-terminal 2'-N-methyl-2'-acylamido-2'-deoxyuridine residue was developed. Unlike their unmethylated counterparts, these oligodeoxynucleotides can be stably immobilized on aldehyde-displaying glass surfaces to provide DNA microarrays. An anthraquinone carboxamido group as a 2'-substituent doubled the capture efficiency of an immobilized tetradecamer.
authors.affiliation: Institute for Organic Chemistry, Universität Karlsruhe (TH), D-76131 Karlsruhe, Germany.
authors.forename: Samy
authors.initials: S
authors.lastname: Al-Rawi
authors.forename: Carolin
authors.initials: C
authors.lastname: Ahlborn
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D015342:DNA Probes / Q000138:chemical synthesis*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D020411:Oligonucleotide Array Sequence Analysis; D014529:Uridine / Q000138:chemical synthesis*
chemical_list: D000880:Anthraquinones; D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D014529:Uridine