title: Biosynthesis of phloroglucinol.
abstract: Substantial concentrations of phloroglucinol were synthesized by Pseudomonas fluorescens Pf-5 expressing the plasmid-localized phlACBDE gene cluster responsible for biosynthesis of 2,4-diacetylphloroglucinol. Expression in Escherichia coli of a single gene in this cluster, P. fluorescens Pf-5 phlD, led to extracellular accumulation of phloroglucinol. Purification of PhlD to homogeneity afforded an enzyme that catalyzed the conversion of malonyl-CoA into phloroglucinol with Km = 5.6 muM and kcat = 10 min-1. Acetylase and deacetylase activities were observed with the catalyzed interconversions of phloroglucinol, 2-acetylphloroglucinol, and 2,4-diacetylphloroglucinol when phlACB was expressed in E. coli. Beyond the mechanistic implications attendant with the identification of an enzyme that catalyzes the conversion of malonyl-CoA into phloroglucinol, PhlD provides the basis for environmentally benign syntheses of phloroglucinol and resorcinol from glucose.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Jihane
authors.initials: J
authors.lastname: Achkar
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.forename: J W
authors.initials: JW
authors.lastname: Frost
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010696:Phloroglucinol / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C096183:PhlD protein, Pseudomonas; D010696:Phloroglucinol