Changes of anti-oxidative enzymes and membrane peroxidation for soil water deficits among 10 wheat genotypes at seedling stage.
Drought is one of the major factors limiting crop production globally, with increasing global climate change making the situation more serious. Wheat is the staple food for more than 35% of world population, so wheat anti-drought physiology study is of importance to wheat production and biological breeding for the sake of coping with abiotic and biotic conditions. Much research is involved in this hot topic, but the pace of progress is not so large because of drought resistance being a multiple-gene-control quantitative character and wheat genome being larger (16,000 Mb). On the other hand, stress adaptive mechanisms are quite different, with stress degree, time course, materials, and experimental plots, thus increasing the complexity of the issue in question. Additionally, a little study is related to the whole life circle of wheat, which cannot provide a comprehensive understanding of its anti-drought machinery. We selected 10 kinds of wheat genotypes as materials, which have potential to be applied in practice, and measured relative change of anti-oxidative enzymes and membrane peroxidation through wheat whole growth-developmental circle (i.e. seedling, tillering and maturing). Here, we firstly reported the results of seedling stage as follows: (1) 10 wheat genotypes can be grouped into three kinds (A-C, respectively) according to their changing trend of the measured indices; (2) A performed better resistance drought under the condition of treatment level 1 (appropriate level), whose activities of anti-oxidative enzymes (POD, SOD, CAT) were higher and MDA lower and chlorophyll a+b higher; (3) B exhibited stronger anti-drought under treatment level 2 (light stress level), whose activities of anti-oxidative enzymes were higher, MDA lower and chlorophyll higher; (4) C expressed anti-drought to some extent under treatment level 3 (serious stress), whose activities of anti-oxidative enzymes were stronger, MDA lower and chlorophyll higher; (5) these results demonstrated that different wheat genotypes have different physiological mechanisms to adapt themselves to changing drought stress, whose molecular basis is discrete gene expression profiling (transcriptom); (6) our results also showed that the concept accepted by most researchers, 70-75% QF is a proper supply for plants, was doubted, because this level could not reflect the true suitable level of wheat. The study in this respect is the key to wheat anti-drought and biological saving-water; (7) our research can provide insights into physiological mechanisms of crop anti-drought and direct practical materials for wheat anti-drought breeding.
Shao
Hong Bo
HB
State Key Laboratory of Soil Erosion and Dryland Farming, The Center of Soil and Water Conservation and Ecoenvironmental Research, Chinese Academy of Sciences, Yangling 712100, People's Republic of China. shaohongbochu@hotmail.com
Liang
Zong Suo
ZS
Shao
Ming An
MA
Wang
Bo Chu
BC
D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D004190:Disasters; D017343:Genes, Plant; D018745:Genome, Plant; D005838:Genotype; D009195:Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D036226:Seedlings / Q000378:metabolism; D012987:Soil; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*; D014867:Water
D000975:Antioxidants; D012987:Soil; D014867:Water; D002734:Chlorophyll; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase