HIC1 promoter methylation and 17p13.3 allelic loss in invasive ductal carcinoma of the breast.
The HIC1 gene is a transcriptional regulator commonly methylated in a variety of human cancer. Thirty-three invasive ductal carcinomas of the breast and 21 matched normal breast tissues were analysed for HIC1 promoter methylation, and allelic loss of a 700 kb region spanning the gene locus. At least one genetic or epigenetic abnormality was found in 27 of the carcinomas tested (82%). Promoter methylation was demonstrated in 21 carcinomas (64%), and nine normal tissues (43%), whereas 18 malignant tumors (54%) showed allelic loss. Concomitant loss of heterozigosity and promoter hypermethylation in the region spanning HIC1 was detected in eight carcinomas (24%) suggesting that in this subset of tumors both copies of the gene are functionally lost. These observations support a role for the HIC1 gene in the pathogenesis of breast ductal carcinomas.
Parrella
Paola
P
Laboratory of Gene Therapy and Oncology, IRCCS, Casa Sollievo della Sofferenza, Viale Padre Pio, 71013 San Giovanni Rotondo (FG), Italy. pparrella@operapadrepio.it
Scintu
Marina
M
Prencipe
Maria
M
Poeta
Maria Luana
ML
Gallo
Antonietta Pia
AP
Rabitti
Carla
C
Rinaldi
Monica
M
Tommasi
Stefania
S
Paradiso
Angelo
A
Schittulli
Francesco
F
Valori
Vanna Maria
VM
Toma
Salvatore
S
Altomare
Vittorio
V
Fazio
Vito Michele
VM
D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D018895:Microsatellite Repeats; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C499808:HIC1 protein, human; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors