title: First preparation of biotinylated gradient polyethylene surface to bind photoactive caged streptavidin.
abstract: A gradient polyethylene (PE) surface was created through corona treatment, in which the corona power increased along the 5 cm length of the PE. The gradient surface was treated with polyethyleneimine and then biotin. Fluorescein-conjugated streptavidin (SAV) caged within 5-carboxymethoxy-2-nitrobenzyl (CMNB) adsorbed onto the biotinylated gradient PE surface following molecular recognition principles. Photoirradiation decomposed the CMNB cage and allowed the fluorescein-conjugated SAV to fluoresce, the intensity of which increased gradually along the PE surface.
authors.affiliation: Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea.
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Kwang Su
authors.initials: KS
authors.lastname: Seo
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.forename: Hai Bang
authors.initials: HB
authors.lastname: Lee
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D009578:Nitrobenzenes / Q000737:chemistry; D010777:Photochemistry; D020959:Polyethylene / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D001710:Biotin; D020959:Polyethylene; D011094:Polyethyleneimine; D019809:Streptavidin