[Biological features of mouse macrophage transfected with Toxoplasma gondii GRA-1 gene].
OBJECTIVE
To investigate the change of biological features of macrophages after transfected by Toxoplasma gondii GRA-1 genes (P24).


METHODS
The transfected cells Cyto-P24-RAW264.7, Nuc-P24-RAW264.7, Mito-P24-RAW264.7 and ER-P24-RAW264.7 were studied by RT-PCR to determine the P24-mRNA expression. Growth features of the cells were examined with microscopy and the cell growth curve was developed.


RESULTS
In four cell lines, expression of ER-P24-RAW 264.7 was found to be higher than the other three, and there was no P24-mRNA expression in either of the cells without P24 insert. The attachment and the proliferation of ER-P24-RAW264.7 were more rapid than normal RAW264.7.


CONCLUSIONS
Transfection of mouse macrophages ER-RAW264. 7 strain with T. gondii P24 gene leads to a prominent change of biological features in the studied cell line.
Peng
Bi-wen
BW
Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 350004, China.
Lin
Jian-yin
JY
Zhang
Tao
T
Jiang
Ming-sen
MS
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014122:Toxoplasma / Q000235:genetics*; D014162:Transfection
D000953:Antigens, Protozoan; C412913:GRA1 antigen; D012333:RNA, Messenger