Differential expression of procollagen lysine 2-oxoglutarate 5-deoxygenase and matrix metalloproteinase isoforms in hypothyroid rat ovary and disintegration of extracellular matrix.
Hypothyroid-induced reproductive malfunction in both the sexes is a common phenomenon of global concern. In an attempt to characterize the differentially expressed genes that might be responsible for these disorders, we have identified a number of clones in hypothyroid rat ovary by subtractive hybridization. One such clone is procollagen lysyl hydroxylase2 (Plod-2), the key enzyme for the first step of collagen biosynthetic pathway, which was down-regulated in hypothyroid condition. We have also demonstrated the reduced expression of other isoforms of Plods, namely Plod-1 and -3 in hypothyroid rat ovary. The current studies are the first of their kind to report that thyroid hormone regulates the Plod gene in rat ovary. Moreover, we have shown the up-regulation of matrix-degrading enzyme(s), matrix metalloproteinase(s) in the hypothyroid rat ovary, whereas the tissue-inhibitory metalloproteinase is down-regulated. Finally, the results of the present studies indicate that in hypothyroid condition, collagen biosynthesis in ovary seems to be disturbed with concomitant enhancement in collagen degradation, resulting in disintegration of overall ovarian structure.
Saha
Samir Kumar
SK
Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
Ghosh
Pamela
P
Konar
Aditya
A
Bhattacharya
Samir
S
Roy
Sib Sankar
SS
D000818:Animals; D003094:Collagen / Q000378:metabolism; D015536:Down-Regulation; D005109:Extracellular Matrix / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000201:enzymology; D007037:Hypothyroidism / Q000201:enzymology* / Q000473:pathology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000379:methods; D010053:Ovary / Q000201:enzymology* / Q000473:pathology; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014284:Triiodothyronine / Q000494:pharmacology
D007527:Isoenzymes; D014284:Triiodothyronine; D003094:Collagen; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D020782:Matrix Metalloproteinases