Effect of high density lipoproteins on protein expression in myoblast cell lines.
We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 microg/mL HDL showed significant differences in the 7-10 pI region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.
Babic
Nikolina
N
University of Washington, Department of Chemistry, P.O. Box 351700, Seattle, Washington 98195, USA.
Beeson
Craig
C
Dovichi
Norman J
NJ
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D008075:Lipoproteins, HDL / Q000502:physiology*; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D008075:Lipoproteins, HDL