Sir2 and the acetyltransferase, Pat, regulate the archaeal chromatin protein, Alba.
The DNA binding affinity of Alba, a chromatin protein of the archaeon Sulfolobus solfataricus P2, is regulated by acetylation of lysine 16. Here we identify an acetyltransferase that specifically acetylates Alba on this residue. The effect of acetylation is to lower the affinity of Alba for DNA. Remarkably, the acetyltransferase is conserved not only in archaea but also in bacteria where it appears to play a role in metabolic regulation. Therefore, our data suggest that S. solfataricus has co-opted this bacterial regulatory system to generate a rudimentary form of chromatin regulation.
Marsh
Victoria L
VL
Medical Research Council Cancer Cell Unit, Hutchison Medical Research Council Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom.
Peak-Chew
Sew Yeu
SY
Bell
Stephen D
SD
D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D005786:Gene Expression Regulation; D019848:Gene Expression Regulation, Archaeal; D008239:Lysine / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D037761:Sirtuins / Q000737:chemistry* / Q000378:metabolism; D048229:Sulfolobus solfataricus / Q000378:metabolism; D013696:Temperature
D019843:Archaeal Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; C444360:Sso10b protein, Sulfolobus solfataricus; D004247:DNA; D000123:Acetyltransferases; D037761:Sirtuins; D008239:Lysine