Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant.
An outbreak of canine parvovirus type 2 infection caused by the Glu-426 mutant in 2 litters of pups is reported. The infected pups (n = 6) were monitored daily for evidence of clinical signs and hematological changes and for the evaluation of viral shedding in the feces. The disease induced by the Glu-426 mutant was mild in all the infected pups. Vomiting and hemorrhagic diarrhea were not observed; however, the pups developed mucoid diarrhea (3.5 median days), depression (1.5 median days), and relative leukopenia and lymphopenia (2.5 median days). Fever and loss of appetite were observed only in 2 pups. Virus was detected in the feces for 4.5, 6.5, and 46 median days by hemagglutination, virus isolation on cell cultures, and real-time polymerase chain reaction (PCR), respectively. By real-time PCR, the highest viral DNA titers were detected in the feces of both litters at day 10, reaching median values of more than 10(10) DNA copies/mg of feces.
Decaro
Nicola
N
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy.
Desario
Costantina
C
Campolo
Marco
M
Elia
Gabriella
G
Martella
Vito
V
Ricci
Dominga
D
Lorusso
Eleonora
E
Buonavoglia
Canio
C
D000818:Animals; D004279:DNA, Viral / Q000737:chemistry; D004196:Disease Outbreaks / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005260:Female; D006386:Hemagglutination Tests / Q000662:veterinary; D007558:Italy / Q000453:epidemiology; D008297:Male; D009154:Mutation; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D017201:Virus Shedding
D004279:DNA, Viral