PICK-1: a scaffold protein that interacts with Nectins and JAMs at cell junctions.
Nectin adhesion molecules are involved in the early steps of cell junction formation. Later during the polarisation process, Nectins are components of epithelial adherens junctions where they are indirectly associated with the E-cadherin/Catenins complex via the adaptator AF-6. To have a better understanding of Nectin-based cell junctions, we looked for some new Nectins' partners. We demonstrate that the scaffold molecule PICK-1, involved in the clustering of junctional receptors in synaptic junctions, interacts directly with Nectins in a PSD-95/Dlg/ZO-1 domain-dependent manner and is localised at adherens junctions in epithelial cells. Finally, we observed that protein interacting with C-kinase-1 (PICK-1) also interacts directly with the junctional adhesion molecules, and we suggest that PICK-1 could be involved in the regulation of both adherens and tight junctions in epithelial cells.
Reymond
Nicolas
N
INSERM UMR.599, Institut de Cancérologie de Marseille, IFR 137, 27 Bvd. Leï-roure, 13009 Marseille, France.
Garrido-Urbani
Sarah
S
Borg
Jean-Paul
JP
Dubreuil
Patrice
P
Lopez
Marc
M
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D062726:Junctional Adhesion Molecules; D000075983:Nectins; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D062726:Junctional Adhesion Molecules; D000075983:Nectins; D009687:Nuclear Proteins; C498297:PICk1 protein, human