High-temperature-induced transposition of insertion elements in burkholderia multivorans ATCC 17616.
An efficient and quantitative method to analyze the transposition of various insertion sequence (IS) elements in Burkholderia multivorans ATCC 17616 was devised. pGEN500, a plasmid carrying a Bacillus subtilis-derived sacB gene, was introduced into ATCC 17616 cells, and 25% of their sucrose-resistant derivatives were found to carry various IS elements on pGEN500. A PCR-based experimental protocol, in which a mixture of several specific primer pairs was used, revealed that pGEN500 captured, in addition to five previously reported IS elements (IS401, IS402, IS406, IS407, and IS408), three novel IS elements, ISBmu1, ISBmu2, and ISBmu3. The global transposition frequency of these IS elements was enhanced more than sevenfold under a high-temperature condition (42 degrees C) but not under oxidative stress or starvation conditions. To our knowledge, this is the first report demonstrating the elevated transposition activities of several IS elements at a high temperature. The efficient experimental protocol developed in this study will be useful in quantitatively and simultaneously investigating various IS elements, as well as in capturing novel functional mobile elements from a wide variety of bacteria.
Ohtsubo
Yoshiyuki
Y
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai 980-8577, Japan. yohtsubo@ige.tohoku.ac.jp
Genka
Hiroyuki
H
Komatsu
Harunobu
H
Nagata
Yuji
Y
Tsuda
Masataka
M
D001483:Base Sequence; D019117:Burkholderia / Q000235:genetics* / Q000254:growth & development*; D003227:Conjugation, Genetic; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004251:DNA Transposable Elements