Epitopes associated with mature spores not recognized on Kudoa thyrsites from recently infected Atlantic salmon smolts.
Atlantic salmon Salmo salar skeletal muscle was examined for Kudoa thyrsites by polymerase chain reaction (PCR) and positive fish were further examined by in situ hybridization (ISH) and immunohistochemistry (IHC). The infection was detected in 42% of salmon by PCR following a 60 d exposure to infective seawater at a temperature of 10 degrees C (= 600 degree-days, degreeD). The parasite was detected by ISH in skeletal and cardiac muscle but not in gill, kidney, spleen, liver, stomach, intestine, pyloric caeca and skin. None of 4 monoclonal antibodies (2F4, 4H2, 1H2, 3E8) raised against mature K. thyrsites spores reacted with the stages identified by ISH following a 600 degreeD exposure, but they did react with ISH-identified stages following a 1600 degreeD exposure. In contrast, a polyclonal antibody reacted with K. thyrsites stages in salmon with both 600 and 1600 degreeD exposures, suggesting that the parasite observed in 600 degreeD infections represents an antigenically distinct developmental stage of K. thyrsites.
Young
Catherine A
CA
Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.
Jones
Simon R M
SR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001955:British Columbia; D015342:DNA Probes; D056890:Eukaryota / Q000235:genetics*; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology*; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D018482:Muscle, Skeletal / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011529:Protozoan Infections, Animal / Q000175:diagnosis*; D020317:Salmo salar; D012623:Seawater / Q000469:parasitology; D033761:Spores, Protozoan / Q000378:metabolism*; D013696:Temperature
D000911:Antibodies, Monoclonal; D015342:DNA Probes