title: Retroviruses and yeast retrotransposons use overlapping sets of host genes.
abstract: A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64 exhibited increased and 66 decreased Ty3 transposition compared with the parental strain. Genes identified in this screen were grouped according to function by using GOnet software developed as part of this study. Gene clusters were related to chromatin and transcript elongation, translation and cytoplasmic RNA processing, vesicular trafficking, nuclear transport, and DNA maintenance. Sixty-six of the mutants were tested for Ty3 proteins and cDNA. Ty3 cDNA and transposition were increased in mutants affected in nuclear pore biogenesis and in a subset of mutants lacking proteins that interact physically or genetically with a replication clamp loader. Our results suggest that nuclear entry is linked mechanistically to Ty3 cDNA synthesis but that host replication factors antagonize Ty3 replication. Some of the factors we identified have been previously shown to affect Ty1 transposition and others to affect retroviral budding. Host factors, such as these, shared by distantly related Ty retroelements and retroviruses are novel candidates for antiviral targets.
authors.affiliation: Department of Biological Chemistry, University of California-Irvine, Irvine, CA 92697, USA.
authors.forename: Becky
authors.initials: B
authors.lastname: Irwin
authors.forename: Michael
authors.initials: M
authors.lastname: Aye
authors.forename: Pierre
authors.initials: P
authors.lastname: Baldi
authors.forename: Nadejda
authors.initials: N
authors.lastname: Beliakova-Bethell
authors.forename: Henry
authors.initials: H
authors.lastname: Cheng
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Dou
authors.forename: Willy
authors.initials: W
authors.lastname: Liou
authors.forename: Suzanne
authors.initials: S
authors.lastname: Sandmeyer
mesh_terms: D015139:Blotting, Southern; D002843:Chromatin / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D034982:Integration Host Factors / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D002843:Chromatin; D018076:DNA, Complementary; D005656:Fungal Proteins; D034982:Integration Host Factors; D018626:Retroelements