title: Functional role of AMP-activated protein kinase in the heart during exercise.
abstract: AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKalpha2 subunit. Exercise increased cardiac AMPKalpha2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.
authors.affiliation: Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Musi
authors.forename: Michael F
authors.initials: MF
authors.lastname: Hirshman
authors.forename: Michael
authors.initials: M
authors.lastname: Arad
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Xing
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Jason
authors.initials: J
authors.lastname: Pomerleau
authors.forename: Ferhaan
authors.initials: F
authors.lastname: Ahmad
authors.forename: Charles I
authors.initials: CI
authors.lastname: Berul
authors.forename: Jon G
authors.initials: JG
authors.lastname: Seidman
authors.forename: Rong
authors.initials: R
authors.lastname: Tian
authors.forename: Laurie J
authors.initials: LJ
authors.lastname: Goodyear
mesh_terms: D055372:AMP-Activated Protein Kinases; D000818:Animals; D004452:Echocardiography; D006321:Heart / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009097:Multienzyme Complexes / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D017346:Protein Serine-Threonine Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases