title: Cardiolipin synthase of Arabidopsis thaliana.
abstract: Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K(m) for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation.
authors.affiliation: RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringerweg 1, 52056 Aachen, Germany.
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowicki
authors.forename: Frank
authors.initials: F
authors.lastname: Müller
authors.forename: Margrit
authors.initials: M
authors.lastname: Frentzen
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D008565:Membrane Proteins / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase