title: In vitro activities of voriconazole, fluconazole, itraconazole and amphotericin B against non Candida albicans yeast isolates.
abstract: Antifungal susceptibility testing was performed on 197 yeast isolates from the BCCM/IHEM biomedical fungi and yeasts collection (Belgian Co-ordinated Collections of Micro-organisms / IPH-Mycology) to study the in vitro activity of voriconazole against fluconazole, itraconazole and amphotericin B. MICs of the four antifungal agents were determined by an adapted NCCLS M27-A microdilution reference method. MIC readings were visually and spectrophotometrically determined. Optical density data were used for calculation of the MIC endpoints. For amphotericin B, the MIC endpoint was defined as the minimal antifungal concentration that exerts 90% inhibition, compared to the control growth. The azoles endpoints were determined at 50% inhibition of growth. The MIC distribution of voriconazole susceptibilities showed that 193 isolates had a MIC < or = 2 microg/ml and 185 a MIC < or = 1 microg/ml. Cross-tabulation of voriconazole, fluconazole, and itraconazole MICs indicated that voriconazole MICs raised with fluconazole and itraconazole MICs. The in vitro data obtained in this study suggest that voriconazole may also be effective treating yeast infection in patients infected with fluconazole or itraconazole resistant isolates.
authors.affiliation: Scientific Institute of Public Health, Mycology Section J, Wytsmanstreet 14, 1050 Brussels, Belgium. d.swinne@iph.fgov.be
authors.forename: Danielle
authors.initials: D
authors.lastname: Swinne
authors.forename: Mirjam
authors.initials: M
authors.lastname: Watelle
authors.forename: Nicole
authors.initials: N
authors.lastname: Nolard
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D041221:Candida glabrata / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D025141:Drug Resistance, Fungal; D026141:Drug Resistance, Multiple, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D017964:Itraconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010843:Pichia / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013045:Species Specificity; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole