title: Expression of lysophosphatidic acid acyltransferase beta (LPAAT-beta) in ovarian carcinoma: correlation with tumour grading and prognosis.
abstract: Lysophosphatidic acid acyltransferase beta (LPAAT-beta) is an enzyme involved in lipid biosynthesis whose role in tumour progression has been of emerging interest in the last few years. We investigated the expression of LPAAT-beta by reverse transcriptase-polymerase chain reaction and immunohistochemistry in 10 ovarian cell lines as well as in a cohort of 106 ovarian tumours and normal ovaries. Lysophosphatidic acid acyltransferase beta mRNA was found in all cell lines and ovarian tumours examined. Expression of LPAAT-beta protein was significantly increased in ovarian carcinomas compared to benign ovarian tissue (chi2 test P-value=0.001, Kruskal-Wallis test P-value <0.0001). Furthermore, LPAAT-beta expression was positively associated with higher tumour grade (P=0.044), higher mitotic index (P<0.0001) and tumour stage (P=0.032). Expression of LPAAT-beta was significantly linked to reduced overall survival time (P=0.024) as well as to shorter progression-free survival time (P=0.012) in patients younger than 60 years. Our study shows that LPAAT-beta is upregulated in ovarian cancer and is more prevalent in poorly differentiated tumours. In addition, LPAAT-beta expression is a predictor of a worse prognosis in patients younger than 60 years. Further studies are needed to investigate if LPAAT-beta may serve as a therapeutic target for certain subgroups of patients.
authors.affiliation: Institute of Pathology, Charité University Hospital, Campus Mitte, Schumannstr. 20/21, 10117 Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Niesporek
authors.forename: C
authors.initials: C
authors.lastname: Denkert
authors.forename: W
authors.initials: W
authors.lastname: Weichert
authors.forename: M
authors.initials: M
authors.lastname: Köbel
authors.forename: A
authors.initials: A
authors.lastname: Noske
authors.forename: J
authors.initials: J
authors.lastname: Sehouli
authors.forename: J W
authors.initials: JW
authors.lastname: Singer
authors.forename: M
authors.initials: M
authors.lastname: Dietel
authors.forename: S
authors.initials: S
authors.lastname: Hauptmann
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000378:metabolism; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase