title: The ectopic expression of phenylalanine ammonia lyase with ectopic accumulation of polysaccharide-linked hydroxycinnamoyl esters in internode parenchyma of rice mutant Fukei 71.
abstract: Both polysaccharide-linked hydroxycinnamoyl esters (PHEs) and lignin are biosynthesized via the phenylpropanoid pathway. In the abnormal internode parenchyma of the rice (Oryza sativa L.) mutant Fukei 71, which has a defective recessive gene (d50), the biosynthesis of lignin and PHEs differs. . The polysaccharide-linked ferulate and p-coumarate have been shown to accumulate to high levels in the irregularly shaped and collapsed internode parenchyma cells of Fukei 71 without an accompanying overaccumulation of lignin as a result of the defective d50 gene. In the present study we demonstrated that in this abnormal parenchyma tissue of Fukei 71 the expression of phenylalanine ammonia lyase (PAL) and glutamine synthetase (GS) were ectopically induced with the ectopic accumulation of PHEs, suggesting that the d50 gene may play a role as a controlling element in the biosynthesis of PHEs during cell-wall formation in the grasses.
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Mase
authors.forename: Kanna
authors.initials: K
authors.lastname: Sato
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nakano
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Nishikubo
authors.forename: Yuuri
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Jinmei
authors.initials: J
authors.lastname: Zhou
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kitano
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D001483:Base Sequence; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers; D004952:Esters; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D012275:Oryza / Q000201:enzymology*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D003373:Coumaric Acids; D017931:DNA Primers; D004952:Esters; D011134:Polysaccharides; D010650:Phenylalanine Ammonia-Lyase; D005974:Glutamate-Ammonia Ligase