title: Differentiation of epithelial Na+ channel function. An in vitro model.
abstract: Confluent monolayers of epithelial cells grown on nonporous support form fluid-filled hemicysts called domes, which reflect active ion transport across the epithelium. Clara-like H441 lung adenocarcinoma cells grown on glass supports and exposed to 50 nM dexamethasone developed domes in a time-dependent fashion. Uplifting of small groups of cells occurred within 6-12 h, well formed domes appeared between 24 and 48 h, and after 7 days, individual domes started to merge. Cells inside of domes compared with those outside domes, or with monolayers not exposed to dexamethasone, differed by higher surfactant production, an increased cytokeratin expression, and the localization of claudin-4 proteins to the plasma membrane. In patch clamp studies, amiloride-blockable sodium currents were detected exclusively in cells inside domes, whereas in cells outside of domes, sodium crossed the membrane through La3+-sensitive nonspecific cation channels. Cells grown on permeable support without dexamethasone expressed amiloride-sensitive currents only after tight electrical coupling was achieved (transepithelial electrical resistance (R(t)) > 1 kilohm). In real-time quantitative PCR experiments, the addition of dexamethasone increased the content of claudin-4, occludin, and Na+ channel gamma-subunit (gamma-ENaC) mRNAs by 1.34-, 1.32-, and 1.80-fold, respectively, after 1 h and was followed by an increase at 6 h in the content of mRNA of alpha- and beta-ENaC and of alpha1- and beta1-Na,K-ATPase. In the absence of dexamethasone, neither change in gene expression nor cell uplifting was observed. Our data suggest that during epithelial differentiation, coordinated expression of tight junction proteins precedes the development of vectorial transport of sodium, which in turn leads to the fluid accumulation in basolateral spaces that is responsible for dome formation.
authors.affiliation: Laboratoire de Physiologie et Physiopathologie, Université Libre de Bruxelles, 1070 Bruxelles, Belgium.
authors.forename: Vadim
authors.initials: V
authors.lastname: Shlyonsky
authors.forename: Arnaud
authors.initials: A
authors.lastname: Goolaerts
authors.forename: Ronald
authors.initials: R
authors.lastname: Van Beneden
authors.forename: Sarah
authors.initials: S
authors.lastname: Sariban-Sohraby
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D017931:DNA Primers; D053503:Epithelial Sodium Channels; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008954:Models, Biological; D016133:Polymerase Chain Reaction; D015222:Sodium Channels / Q000378:metabolism / Q000502:physiology*
chemical_list: D017931:DNA Primers; D053503:Epithelial Sodium Channels; D005456:Fluorescent Dyes; D015222:Sodium Channels