[Detection and classification of Mycoplasma in cell lines].
OBJECTIVE
To detect the rate of Mycoplasma infection in cell lines and further determine its types.


METHODS
We performed nest PCR amplification of Mycoplasma's conserved regions (16S-23S) and sequenced the spacer with different length between conserved regions.


RESULTS
Within the tested 22 cell lines, 17 (77.3%) showed Mycoplasma infections, of which 5 had two or more types of Mycoplasma. M. fermentans and hyorhinis were more frequently detected within the types of infected Mycoplasma.


CONCLUSIONS
The high rate of Mycoplasma infection in cell lines makes it necessary for researchers to pay more attention to its influence on research data when using cell lines as models. Establishment of detection and classifying techniques make it possible to further study the pathogenesis of different types of Mycoplasma.
Dai
Yue
Y
Department of Genetic, Peking University School of Oncology, Beijing 100034, China.
Lü
Yun-wei
YW
Zhang
Chun-feng
CF
Cao
Bang-wei
BW
Chai
Qing-bo
QB
Ma
Ming-xin
MX
Ke
Yang
Y
D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification* / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology; D016858:Mycoplasma fermentans / Q000302:isolation & purification; D045790:Mycoplasma hyorhinis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S