title: Small-angle X-ray scattering on solutions of carboxymethylcellulose and bovine serum albumin.
abstract: The existence of attractive interaction between CMC and BSA was evidenced in solution at pH higher than the protein isoelectric point by means of SAXS. Mixtures of BSA at 10 x 10(-3) g . mL(-1) and CMC at the concentration range of 1 x 10(-3) g . mL(-1) to 10 x 10(-3) g . mL(-1) were investigated. Upturns in the very low q range revealed the presence of aggregates when the CMC concentration was higher than 2 x 10(-3) g . mL(-1). The comparison between the calculated form factor with the experimental curves at intermediate and long q values indicated that the BSA molecules keep their native form in mixtures with CMC concentrations lower than 5 x 10(-3) g . mL(-1). Therefore, for CMC concentrations higher than 2 x 10(-3) g . mL(-1) the mixtures start to present aggregates and free BSA molecules coexisting in solution.
authors.affiliation: Instituto de Química-USP, P.O. Box 26077, 05513-970 São Paulo, S.P. Brazil.
authors.forename: Sabrina M
authors.initials: SM
authors.lastname: Pancera
authors.forename: Rosangela
authors.initials: R
authors.lastname: Itri
authors.forename: Denise F S
authors.initials: DF
authors.lastname: Petri
mesh_terms: D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014965:X-Rays
chemical_list: D012996:Solutions; D012710:Serum Albumin, Bovine; D002266:Carboxymethylcellulose Sodium