Evolutionary diversification of TTX-resistant sodium channels in a predator-prey interaction.
Understanding the molecular genetic basis of adaptations provides incomparable insight into the genetic mechanisms by which evolutionary diversification takes place. Whether the evolution of common traits in different lineages proceeds by similar or unique mutations, and the degree to which phenotypic evolution is controlled by changes in gene regulation as opposed to gene function, are fundamental questions in evolutionary biology that require such an understanding of genetic mechanisms. Here we identify novel changes in the molecular structure of a sodium channel expressed in snake skeletal muscle, tsNa(V)1.4, that are responsible for differences in tetrodotoxin (TTX) resistance among garter snake populations coevolving with toxic newts. By the functional expression of tsNa(V)1.4, we show how differences in the amino-acid sequence of the channel affect TTX binding and impart different levels of resistance in four snake populations. These results indicate that the evolution of a physiological trait has occurred through a series of unique functional changes in a gene that is otherwise highly conserved among vertebrates.
Geffeney
Shana L
SL
Department of Biology, Utah State University, Logan, Utah 84322-5305, USA. geffeney@biology.usu.edu
Fujimoto
Esther
E
Brodie
Edmund D
ED
Brodie
Edmund D
ED
Ruben
Peter C
PC
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D017812:Colubridae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004553:Electric Conductivity; D006801:Humans; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010802:Phylogeny; D011235:Predatory Behavior / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012455:Salamandridae / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D026941:Sodium Channel Blockers / Q000378:metabolism / Q000494:pharmacology; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013779:Tetrodotoxin / Q000378:metabolism / Q000494:pharmacology*; D014982:Xenopus laevis
D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin; D012964:Sodium