The enhancement of preproendothelin-1 synthesis and the acceleration of endothelin-1 processing in the acute hypoxic rat aorta.
Wistar rats were deeply anesthetized and perfused by Hanks' solution bubbled with either 95% air and 5% CO2 (normoxic group) or 95% N2 and 5% CO2 (hypoxic group) from the thoracic aorta for 30 minutes. The isolated abdominal aortas were used for electron microscopy, immunocytochemistry of endothelin-1 (ET-1) and endothelin-converting enzyme-1 (ECE-1), and in situ hybridization of preproendothelin-1 mRNA. A remarkable increase in the number of Weibel-Palade bodies, storage sites of ET-1 and ECE-1, occurred in the hypoxic group when compared with the normoxic group. Immunoreactivities for ET-1 and ECE-1, and signals for preproendothelin-1 mRNA were seen along the endothelia of both groups, but the intensities were significantly elevated in the hypoxic group. The increase in the number of ECE-1 immunoreactive gold particles was noticed in Weibel-Palade bodies in the hypoxic group. These findings indicate the enhancement of preproendothelin-1 synthesis in the rat aortic endothelial cells and the acceleration of ET-1 processing in Weibel-Palade bodies of such cells in an acute hypoxic condition.
Doi
Yoshiaki
Y
Department of Anatomy, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan. y-doi@med.uoeh-u.ac.jp
Kudo
Hideaki
H
Nishino
Tomoko
T
Nagata
Takahisa
T
Fujimoto
Sunao
S
D000208:Acute Disease; D000818:Animals; D001012:Aorta, Abdominal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D000072396:Endothelin-Converting Enzymes; D000860:Hypoxia / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D020302:Weibel-Palade Bodies / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D019332:Endothelin-1; D011498:Protein Precursors; D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes