title: De novo design of a redox-active minimal rubredoxin mimic.
abstract: Metal-binding sites in metalloproteins frequently occur at the interfaces of elements of secondary structure, which has enabled the retrostructural analysis of natural proteins and the de novo design of helical bundles that bind metal ion cofactors. However, the design of metalloproteins containing beta-structure is less well developed, despite the frequent occurrence of beta-conformations in natural metalloproteins. Here, we describe the design and construction of a beta-protein, RM1, that forms a stable, redox-active 4-Cys thiolate Fe(II/III) site analogous to the active site of rubredoxin. The protein folds into a beta-structure in the presence and absence of metal ions and binds Fe(II/III) to form a redox-active site that is stable to repeated cycles of oxidation and reduction, even in an aerobic environment.
authors.affiliation: Department of Biochemistry and Biophysics, Johnson Foundation, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, Japan.
authors.forename: Vikas
authors.initials: V
authors.lastname: Nanda
authors.forename: Michael M
authors.initials: MM
authors.lastname: Rosenblatt
authors.forename: Artur
authors.initials: A
authors.lastname: Osyczka
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kono
authors.forename: Zelleka
authors.initials: Z
authors.lastname: Getahun
authors.forename: P Leslie
authors.initials: PL
authors.lastname: Dutton
authors.forename: Jeffery G
authors.initials: JG
authors.lastname: Saven
authors.forename: William F
authors.initials: WF
authors.lastname: Degrado
mesh_terms: D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry; D002942:Circular Dichroism; D003035:Cobalt / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry*; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D020027:Pyrococcus furiosus / Q000737:chemistry; D012416:Rubredoxins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D010446:Peptide Fragments; D012416:Rubredoxins; D003035:Cobalt; D014364:Tryptophan; D015032:Zinc; D003545:Cysteine