[The effect of kanglaite injection(KLT) on the proliferation and telomerase activity of rat mesangial cells].
OBJECTIVE
To observe the effect of Kanglaite injection(KLT) on the proliferation and telomerase activity of mesangial cells in rats.


METHODS
MTT, telomere repeat amplification protocal (TRAP), ELISA, PAGE and silver-stain were applied to detect the growth rate and telomerase activity of MC after stimulation of KLT and IL-1.


RESULTS
The growth rate of MC was enhanced by IL-1 stimulation, which was accompanied with a redection of the activity of telomerase. Adversely, the growth rate of MC was reduced by KLT, which was accompanied with an enhancement of activity of telomerase. Moreover, the growth rate of MC and the activity of telomerase were both inhibited by the combinative use of IL-1 and KLT without any influence from the sequence of their administration.


CONCLUSIONS
KLT could inhibit proliferation and telomerase activity of MC with or without pre-stimulation with IL-1. KLT might be useful to prevent and treat glomerular nephritis related to MC proliferation.
Hu
Ying
Y
Second Affiliated Hospital, The Medical College of Zhejiang University, Hangzhou 310009, China. hy-doctor@163.com
Liang
Hua
H
Gong
Wei-Kun
WK
Xu
Ze-Feng
ZF
Zou
Qing-Ling
QL
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D031742:Coix / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology*; D007267:Injections; D010938:Plant Oils / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D012639:Seeds / Q000737:chemistry; D019098:Telomerase / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D010938:Plant Oils; D019098:Telomerase