title: rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens.
abstract: We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.
authors.affiliation: Laboratory of Molecular Parasitology, Lindsley F. Kimball Research Institute, The New York Blood Center, 310 East 67th Street, New York, NY 10021, USA. angus.macdonald@hepcgen.com
authors.forename: Angus J
authors.initials: AJ
authors.lastname: MacDonald
authors.forename: Long
authors.initials: L
authors.lastname: Cao
authors.forename: Yuxian
authors.initials: Y
authors.lastname: He
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Shibo
authors.initials: S
authors.lastname: Jiang
authors.forename: Sara
authors.initials: S
authors.lastname: Lustigman
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects* / Q000276:immunology; D015801:Helminth Proteins / Q000494:pharmacology*; D051379:Mice; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000276:immunology; D022142:Secretory Vesicles / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D015801:Helminth Proteins; C414408:Ov-ASP-1 protein, Onchocerca volvulus; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D010047:Ovalbumin