Immobilization of beta-fructofuranosidase from Aspergillus japonicus on chitosan using tris(hydroxymethyl)phosphine or glutaraldehyde as a coupling agent.
A partially purified beta-fructofuranosidase from Aspergillus japonicus was covalently immobilized on to chitosan beads using either glutaraldehyde or tris(hydroxymethyl)phosphine (THP) as a coupling agent. Compared with the glutaraldehyde-immobilized and the free enzyme, the THP-immobilized enzyme had the highest thermal stability with 78% activity retained after 12 days at 37 degrees C. The THP-immobilized enzyme also had higher reusability than that immobilized by glutaraldehyde, 75% activity was retained after 11 batches (or 11 days) at 37 degrees C for the THP immobilized enzyme system. Less yield (48%) of fructooligosaccharides (FOS) were produced by the THP-immobilized enzyme compared with the free enzyme system (58%) from 50 (w/v) sucrose at 50 degrees C.
Cheng
Tzu-Chien
TC
Department of Bioengineering, Tatung University, Taipei 104, Taiwan.
Duan
Kow-Jen
KJ
Sheu
Dey-Chyi
DC
D001230:Aspergillus / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010720:Phosphines / Q000737:chemistry / Q000494:pharmacology*; D013395:Sucrose / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000737:chemistry*
D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D009943:Organophosphorus Compounds; D010720:Phosphines; D002686:Chitin; D013395:Sucrose; D048271:Chitosan; D043324:beta-Fructofuranosidase; C044646:phosphine; D005976:Glutaral; C505069:tris(hydroxymethyl)phosphine