title: Evaluation of antimicrobial susceptibility of bacteria containing the qnr gene and FOX-5 beta-lactamase by four automated systems.
abstract: The accuracy and performance of four automated instruments (BD Phoenix, MicroScan WalkAway, VITEK-2 and Wider) were evaluated for susceptibility testing of fluoroquinolones and beta-lactams with four clinical isolates of Klebsiella pneumoniae and the corresponding Escherichia coli transconjugants containing a plasmid carrying the qnr gene and coding for FOX-5 production. No major or very major errors were detected with the MicroScan system. Many of the minor errors for both quinolones and beta-lactams clustered around the intermediate breakpoints.
authors.affiliation: Microbiology, School of Medicine, University of Seville, Seville, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez-Martinez
authors.forename: M C
authors.initials: MC
authors.lastname: Conejo
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Martínez
authors.forename: M E
authors.initials: ME
authors.lastname: Cano
authors.forename: C
authors.initials: C
authors.lastname: Velasco
authors.forename: A
authors.initials: A
authors.lastname: Pascual
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001331:Automation / Q000592:standards; D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D014162:Transfection; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; C485384:Qnr protein, E coli; C485385:Qnr protein, Klebsiella pneumoniae; D011933:Reagent Kits, Diagnostic; D047090:beta-Lactams; C444464:beta-lactamase FOX-5; D001618:beta-Lactamases