RNA duplexes with biphenyl substituents as base replacements are less stable than DNA duplexes.
Introduction of a hydrophobic biphenyl-C-nucleotide pair into a 11-mer RNA duplex is associated with a net penalty in the free energy of duplex formation of 2.0 kcal mol(-1) or 10 degrees C in Tm, relative to DNA. These differential stabilities are of relevance with respect to the transcriptional and translational aspects of hydrophobic base-pairs.
Brotschi
Christine
C
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
Leumann
Christian J
CJ
D020029:Base Pairing; D001713:Biphenyl Compounds / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D044366:Transition Temperature / Q000528:radiation effects
D001713:Biphenyl Compounds; D004247:DNA; D012313:RNA; C010574:biphenyl