Photochemical generation of ribose abasic sites in RNA oligonucleotides.
[reaction: see text] A general method for the photochemical generation of ribose abasic sites within RNA oligonucleotides is reported. Photochemically caged nucleoside phosphoramidite analogues were prepared and incorporated into RNA oligonucleotides by automated RNA synthesis. Irradiation of the modified RNA at 350 nm efficiently produced ribose abasic sites at specific sites within RNA sequences. The current approach offers a chemical route to RNA abasic lesions for RNA biochemical studies.
Trzupek
John D
JD
Department of Chemistry and The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
Sheppard
Terry L
TL
D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry / Q000379:methods*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D012313:RNA