D: -Mannitol formation from D: -glucose in a whole-cell biotransformation with recombinant Escherichia coli.
Recently, we reported on the construction of a whole-cell biotransformation system in Escherichia coli for the production of D: -mannitol from D: -fructose. Supplementation of this strain with extracellular glucose isomerase resulted in the formation of 800 mM D: -mannitol from 1,000 mM D: -glucose. Co-expression of the xylA gene of E. coli in the biotransformation strain resulted in a D: -mannitol concentration of 420 mM from 1,000 mM D: -glucose. This is the first example of conversion of D: -glucose to D: -mannitol with direct coupling of a glucose isomerase to the biotransformation system.
Kaup
Björn
B
Karl-Winnacker-Institut, Bioverfahrenstechnik, DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt, Germany.
Bringer-Meyer
Stephanie
S
Sahm
Hermann
H
D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D005947:Glucose / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D008354:Mannitol Dehydrogenases / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D008353:Mannitol; D008354:Mannitol Dehydrogenases; D005560:Formate Dehydrogenases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose