title: Chromosomal instability in meningiomas.
abstract: Approximately 60% of sporadic meningiomas are caused by inactivation of the NF2 tumor suppressor gene on chromosome 22. No causative gene is known for the remaining 40%. Cytogenetic analysis shows that meningiomas caused by inactivation of the NF2 gene can be divided into tumors that show monosomy 22 as the sole abnormality and tumors with a more complex karyotype. Meningiomas not caused by the NF2 gene usually have a diploid karyotype. Here we report that, besides the clonal chromosomal aberrations, the chromosome numbers in many meningiomas varied from one metaphase spread to the other, a feature that is indicative of chromosomal instability. Unexpectedly and regardless of genotype, a subgroup of tumors was observed with an average number of 44.9 chromosomes and little variation in the number of chromosomes per metaphase spread. In addition, a second subgroup was recognized with a hyperdiploid number of chromosomes (average 48.5) and considerable variation in numbers per metaphase. However, this numerical instability resulted in a clonal karyotype with chromosomal gains and losses in addition to loss of chromosome 22 only in meningiomas caused by inactivation of the NF2 gene. In cultured cells of all tumor groups, bi- and multinucleated cells were seen, as well as anaphase bridges, residual chromatid strings, multiple spindle poles, and unseparated chromatids, suggesting defects in the mitotic apparatus or kinetochore. Thus, we conclude that even a benign and slow-growing tumor like a meningioma displays chromosomal instability.
authors.affiliation: Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: Angela A G
authors.initials: AA
authors.lastname: van Tilborg
authors.forename: Bushra
authors.initials: B
authors.lastname: Al Allak
authors.forename: Sandra C J M
authors.initials: SC
authors.lastname: Velthuizen
authors.forename: Annie
authors.initials: A
authors.lastname: de Vries
authors.forename: Johan M
authors.initials: JM
authors.lastname: Kros
authors.forename: Cees J J
authors.initials: CJ
authors.lastname: Avezaat
authors.forename: Annelies
authors.initials: A
authors.lastname: de Klein
authors.forename: H Berna
authors.initials: HB
authors.lastname: Beverloo
authors.forename: Ellen C
authors.initials: EC
authors.lastname: Zwarthoff
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D002892:Chromosomes, Human, Pair 22; D016515:Genes, Neurofibromatosis 2; D006801:Humans; D007621:Karyotyping; D019656:Loss of Heterozygosity; D008577:Meningeal Neoplasms / Q000145:classification / Q000235:genetics*; D008579:Meningioma / Q000145:classification / Q000235:genetics*; D008875:Middle Aged