Pattern generation of biological ligands on a biodegradable poly(glycolic acid) film.
The micropatterns of biological ligands (biotin and RGD peptides) were generated on a flat surface of biodegradable polymer, poly(glycolic acid) (PGA). The immobilization of biological ligands onto the surface of biodegradable polymers (especially aliphatic polyesters) is usually hampered by the absence of functionalizable groups on the polymer backbone. We demonstrate herein that PGA polymer films were modified by surface hydrolysis to introduce carboxylic acid groups on the film surfaces, which were subsequently used for patterning amine-terminated ligands by microcontact printing. Fluorescence microscopy was used to verify the pattern of biotin on the surface of the PGA films after complexation with fluorescein-conjugated streptavidin. In addition, the cellular micropatterns were obtained from micropatterns of RGD peptides on the surface-hydrolyzed PGA films.
Lee
Kyung-Bok
KB
Kim
Dong Jin
DJ
Lee
Zee-Won
ZW
Woo
Seong Ihl
SI
Choi
Insung S
IS
D001673:Biodegradation, Environmental; D001710:Biotin / Q000138:chemical synthesis*; D006868:Hydrolysis; D008024:Ligands; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force / Q000379:methods; D009842:Oligopeptides / Q000138:chemical synthesis*; D011100:Polyglycolic Acid / Q000737:chemistry*; D013499:Surface Properties
D008024:Ligands; D008567:Membranes, Artificial; D009842:Oligopeptides; D011100:Polyglycolic Acid; D001710:Biotin