2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose induces apoptosis in Hep G2 cells.
OBJECTIVE
To determine the effect of 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose (COPADG) on proliferation and apoptosis of human hepatocellular carcinoma cells (Hep G2).


METHODS
Hep G2 cells were cultured in RPMI-1640 medium in the presence of various concentrations of COPADG. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.


RESULTS
At the concentration ranging between 1-30 microM, COPADG potently inhibits growth and induce apoptosis of Hep G2 cells.


CONCLUSIONS
COPADG could effectively induce apoptosis in Hep G2 cells. It may be potentially useful as a new agent for treatment of human hepatocellular carcinoma.
Wu
Jing
J
Department of Gastroenterology and Hepatology, the First Hospital of Lanzhou University, Lanzhou 730000, China.
Qi
Delu
D
Wei
Kou
K
Li
Juan
J
Chen
Xuan
X
Wang
Aiqing
A
Liu
Weiming
W
Xue
Qunji
Q
Qiao
Liang
L
D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D046529:Microscopy, Electron, Transmission
D003847:Deoxyglucose