title: [Study on the interaction of metal ion in active center of superoxide dismutase with cobalt chloride by visible spectroscopy].
abstract: The direct interaction of metal ion in active center of copper-zinc superoxide dismutase (Cu2Zn2SOD) with inorganic cobalt chloride (CoCl2) in buffer has been first studied by visible spectroscopy. The effects of increasing amounts of CoCl2, changing pH,different acid salt and equilibrium time on the interaction have been discussed. It was found that the Co(II) of CoCl2 makes an exchanging interaction with Zn(II) in Cu2Zn2SOD and thus the partial Cu2Co2SOD is formed.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023 Dalian.
authors.forename: X
authors.initials: X
authors.lastname: Zheng
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Cheng
mesh_terms: D002021:Buffers; D020134:Catalytic Domain; D003035:Cobalt / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D002021:Buffers; D003035:Cobalt; D013482:Superoxide Dismutase; C018021:cobaltous chloride; D015032:Zinc