title: Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry.
abstract: In the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis.
authors.affiliation: Max-Planck-Institute for Molecular Genetics, Berlin, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Giavalisco
authors.forename: Eckhard
authors.initials: E
authors.lastname: Nordhoff
authors.forename: Thomas
authors.initials: T
authors.lastname: Kreitler
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Klöppel
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Joachim
authors.initials: J
authors.lastname: Klose
authors.forename: Johan
authors.initials: J
authors.lastname: Gobom
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000502:physiology; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029681:Arabidopsis Proteins; D020033:Protein Isoforms; D020543:Proteome