[Proteome analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in K562 cells].
OBJECTIVE
To investigate arsenic trioxide (As(2)O(3))-target interactions at the level of nuclear matrix (NM) in chronic myelogenous leukemia cell line K562 by proteomics.


METHODS
DNA fragmentation analysis was used for As(2)O(3) induced apoptosis of K562 cells. The nuclear matrix proteins were analyzed by high-resolution two-dimensional gel electrophoresis and computer-assisted image analysis.


RESULTS
While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots were found characteristic of As(2)O(3) treated cells. Onset of mass mange apoptosis, and the profiling of nuclear matrix proteins had been alternated and it was a more sensitive indicator than nucleosomal DNA fragmentation against As(2)O(3) treatment.


CONCLUSIONS
As(2)O(3) induced apoptosis in K562 cells in a dose-time-dependent manner. As(2)O(3) might be clinically useful in treatment of chronic myelogenous leukemia and the changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for the treatment.
Wang
Zi-hui
ZH
Department of Pathology, Peking University Shenzhen Hospital, Shenzhen 518036, China.
Yu
Ding
D
Zheng
Jie
J
Chen
Yan
Y
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D040901:Proteomics
D000970:Antineoplastic Agents; D001152:Arsenicals; D034521:Nuclear Matrix-Associated Proteins; D010087:Oxides; D000077237:Arsenic Trioxide