Adherence and colonization properties of Lactobacillus rhamnosus TB1, a broiler chicken isolate.
OBJECTIVE
Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A selected strain showing a good adherence in BMM test was used for in vivo colonization assays.


RESULTS
In vitro assessment of adhesion of broiler chicken isolates was performed using BMM assay. Among LAB strains tested, Lactobacillus rhamnosus TB1 showed a good adherence that was comparable to the one of an Escherichia coli EPEC strain used as positive control. For in vivo colonization assays this strain was fluorescently stained with the carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) thus allowing its detection in different layers of intestinal tract after inoculation in broiler chicken. Further, stained L. rhamnosus were found with a highest value in rectum, jejunum and ileum both 3 and 24 h after administration.


CONCLUSIONS
BMM assay is a quick method to test in vitro adhesion properties of bacterial strains and cFDA-SE-stained bacteria may be considered as an alternative method to test in vivo adhesion and colonization properties.


CONCLUSIONS
Lactobacillus rhamnosus TB1 was therefore showed to be able to adhere strongly in vitro to BMM and in vivo to intestinal epithelial cells of chicken and may be considered as a potential probiotic for chicken.
Bouzaine
T
T
UR-Microbial and Food Process, National Institute of Applied Science and Technology, University of Carthage, Tunis, Tunisia.
Dauphin
R D
RD
Thonart
Ph
P
Urdaci
M C
MC
Hamdi
M
M
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D002645:Chickens / Q000382:microbiology*; D003094:Collagen / Q000378:metabolism; D015169:Colony Count, Microbial; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D007797:Laminin / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen