The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.
The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues.
Shirasawa-Seo
Naomi
N
Miyagi Prefectural Agriculture and Horticulture Research Center, Takadate-kawakami, Natori, Miyagi, Japan.
Sano
Yoshitaka
Y
Nakamura
Shigeo
S
Murakami
Taka
T
Gotoh
Yoko
Y
Naito
Yumi
Y
Hsia
Chi Ni
CN
Seo
Shigemi
S
Mitsuhara
Ichiro
I
Kosugi
Shunichi
S
Ohashi
Yuko
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D025682:Nanovirus / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D014764:Viral Proteins