title: [Mechanism of dracorhodin perchlorate-induced Hela cell apoptosis].
abstract: OBJECTIVE
  To study the mechanism of dracorhodin perchlorate-induced Hela cell apoptosis.
  
  
  METHODS
  Cell viability was measured by MTT method. Morphological changes were observed by phase contrast microscopy and Hoechst 33258 staining. DNA fragmentation was assayed by agarose gel electrophoresis. Protein expression was detected by Western blot analysis.
  
  
  RESULTS
  Dracorhodin perchlorate induced Hela cell apoptosis. The apoptosis was partially reversed by caspase-1, -3, -8, -9 and caspase family inhibitors. Treatment of Hela cells with dracorhodin perchlorate for 12 h increased the protein expression ratio of Bax/Bcl-XL; procaspase-3, -8, ICAD and PARP were cleaved to smaller molecules.
  
  
  CONCLUSIONS
  Dracorhodin perchlorate induced Hela cell death via alteration of Bax/Bcl-XL ratio and activation of caspases.
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ming-yu
authors.initials: MY
authors.lastname: Xia
authors.forename: Min-wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Hao-ran
authors.initials: HR
authors.lastname: Wang
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tashiro
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D028023:Arecaceae / Q000737:chemistry; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry; D011506:Proteins / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D001578:Benzopyrans; D061945:Caspase Inhibitors; D004365:Drugs, Chinese Herbal; D011506:Proteins; C109664:caspase-activated DNase inhibitor; C060327:dracorhodin; D020169:Caspases