Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4-sulfophenyl isothiocyanate.
Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4-sulfophenyl isothiocyanate derivatives, generated by matrix-assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed.
Oh
Joo Yeon
JY
National Creative Research Initiative Center for Control of Reaction Dynamics and School of Chemistry, Seoul National University, Korea.
Moon
Jeong Hee
JH
Lee
Yong Ho
YH
Hyung
Seok-Won
SW
Lee
Sang-Won
SW
Kim
Myung Soo
MS
D000595:Amino Acid Sequence; D001557:Benzenesulfonates / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013861:Thiocyanates / Q000737:chemistry*
D001557:Benzenesulfonates; D017879:Isothiocyanates; D010455:Peptides; D013861:Thiocyanates; C005441:phenylisothiocyanate; C015308:4-isothiocyanatobenzenesulfonate