title: Investigation of the interaction between peanut agglutinin and synthetic glycopolymeric multivalent ligands.
abstract: The interaction between synthetic glycoplymers bearing beta-D-galactose side groups and the lectin peanut agglutinin (PNA) was investigated by UV-difference spectroscopy and isothermal titration calorimetry (ITC). UV-difference spectroscopy indicated that the polymer-lectin interaction was stronger than that between PNA and either the corresponding monomer, D-galactose or D-lactose. The thermodynamics of binding (K, DeltaG, DeltaH, DeltaS and n) were determined from ITC data by fitting with a two-site, non-cooperative binding model. It was found that the glycopolymer displayed around a 50 times greater affinity for the lectin than the parent carbohydrate, and around 10 times greater than the monomer, on a valency-corrected basis. Binding was found to be entropically driven, and was accompanied by aggregation and precipitation of protein molecules. Furthermore, interesting differences between polymers prepared either from deacetylated monomers, or by deacetylation of pre-formed polymers, were found.
authors.affiliation: Department of Chemistry and Interdisciplinary Research Centre in Polymer Science and Technology, University of Durham, UK.
authors.forename: Moira
authors.initials: M
authors.lastname: Ambrosi
authors.forename: Neil R
authors.initials: NR
authors.lastname: Cameron
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
authors.forename: Snjezana
authors.initials: S
authors.lastname: Stolnik
mesh_terms: D005685:Galactans / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D008024:Ligands; D015394:Molecular Structure; D019887:Peanut Agglutinin / Q000737:chemistry*; D013057:Spectrum Analysis; D013816:Thermodynamics
chemical_list: D005685:Galactans; D008024:Ligands; D019887:Peanut Agglutinin; D005690:Galactose