Polarised distribution of the RNA-binding protein Staufen in differentiated intestinal epithelial cells.
mRNA localisation, as a mechanism for directing localised protein synthesis, plays a vital role in the functioning of certain cells, such as neurons and oocytes. Potentially this mechanism may also occur in polarised intestinal epithelial cells. Here we show that Staufen1(55), a protein involved in mRNA localisation and transport, is asymmetrically distributed in differentiated Caco-2 intestinal epithelial cells and partly co-localised with calnexin, a marker of the endoplasmic reticulum. The localisation to the apical region of the cell indicates that Staufen may be involved in localisation of transcripts to this domain.
Gautrey
Hannah
H
Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK.
McConnell
Josie
J
Hall
Judith
J
Hesketh
John
J
D018938:Caco-2 Cells; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
D003598:Cytoskeletal Proteins; D016601:RNA-Binding Proteins; C492185:STAU1 protein, human