c-src activating mutation analysis in Chinese patients with colorectal cancer.
OBJECTIVE
To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases.


RESULTS
Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found.


CONCLUSIONS
c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
Tan
Ye-Xiong
YX
International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Institute, Shanghai 200438, China.
Wang
Han-Tao
HT
Zhang
Peng
P
Yan
Zhong-Hua
ZH
Dai
Guan-Long
GL
Wu
Meng-Chao
MC
Wang
Hong-Yang
HY
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016391:Genes, src / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics / Q000556:secondary; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational