title: Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential.
abstract: The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.
authors.affiliation: Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wu
authors.forename: Mu De
authors.initials: MD
authors.lastname: Shi
authors.forename: Rui Fu
authors.initials: RF
authors.lastname: Yang
authors.forename: You Yu
authors.initials: YY
authors.lastname: He
authors.forename: Chao
authors.initials: C
authors.lastname: Bian
authors.forename: Tie Liu
authors.initials: TL
authors.lastname: Shi
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Xue-Liang
authors.initials: XL
authors.lastname: Zhu
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Jiang
authors.forename: Yi Xue
authors.initials: YX
authors.lastname: Li
authors.forename: Lin-Chen
authors.initials: LC
authors.lastname: Yan
authors.forename: Yong Yong
authors.initials: YY
authors.lastname: Ji
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Guo-Mei
authors.initials: GM
authors.lastname: Lin
authors.forename: Lin
authors.initials: L
authors.lastname: Tian
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Hong Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: You Hua
authors.initials: YH
authors.lastname: Xie
authors.forename: Gang
authors.initials: G
authors.lastname: Pei
authors.forename: Jia Rui
authors.initials: JR
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D019295:Computational Biology; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D014764:Viral Proteins