[Study on optimizing the analysis condition for active compound in Eucommia olive].
The absorption spectroscopic determination of aucubin by condensation with p-dimethylaminobenzaldehyde was optimized in this paper. A high sensitive method was established and the linearity range of the method was from 4.5 to 45 microg x mL(-1). The influences of the factors, such as the dosage of coloring agent, reaction time, heating temperature and the dose of acid etc., on the system were studied. And the result indicated that all of the factors had obvious effects on the system. The method has been applied to determining the content of aucubin in the extract of Eucommia olive with satisfactory results.
Peng
Mi-jun
MJ
Jishou University, Zhangjiajie 427000, China.
Zhou
Chun-shan
CS
Liu
Jian-lan
JL
Zhang
Min
M
Lei
Qi-fu
QF
D002384:Catalysis; D004304:Dosage Forms; D031283:Eucommiaceae / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis; D013501:Surface-Active Agents / Q000032:analysis*; D013696:Temperature; D013997:Time Factors
D004304:Dosage Forms; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D004364:Pharmaceutical Preparations; D013501:Surface-Active Agents; C006650:aucubin; D006861:Hydrogen Peroxide