title: Assembly of hereditary amyloid beta-protein variants in the presence of favorable gangliosides.
abstract: The mechanisms underlying regional amyloid beta-protein (Abeta) deposition in brain remain unclear. Here we show that assembly of hereditary variant Dutch- and Italian-type Abetas, and Flemish-type Abeta was accelerated by GM3 ganglioside, and GD3 ganglioside, respectively. Notably, cerebrovascular smooth muscle cells, which compose the cerebral vessel wall at which the Dutch- and Italian-type Abetas deposit, exclusively express GM3 whereas GD3 is upregulated in the co-culture of endothelial cells and astrocytes, which forms the cerebrovascular basement membrane, the site of Flemish-type Abeta deposition. Our results suggest that regional Abeta deposition is induced by the local gangliosides in the brain.
authors.affiliation: Department of Alzheimer's Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Obu 474-8522, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hirabayashi
authors.forename: Masakuni
authors.initials: M
authors.lastname: Amari
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Galina
authors.initials: G
authors.lastname: Romanov
authors.forename: William E
authors.initials: WE
authors.lastname: Van Nostrand
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Yanagisawa
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D016229:Amyloid beta-Peptides; D005732:Gangliosides