Characterization of p24 gene of Spodoptera litura multicapsid nucleopolyhedrovirus.
Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) p24 gene is 753 bp long, potentially encoding 244 amino acids with a predicted molecular weight of 27.3 kDa. Homology analysis indicated that SpltMNPV P24 has 20-36% amino acid identity with that of other known baculoviruses. RT-PCR results showed that the p24 gene is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infected S. litura cells detected a specific 28 kDa protein, and this protein was not N-glycosylated. Structural localization revealed that SpltMNPV P24 was associated with the nucleocapsid of occlusion-derived virus (ODV) as a complex form of 83 kDa.
Li
Zhaofei
Z
State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou, 510275, P.R.China.
Li
Cuifeng
C
Pan
Lijing
L
Yu
Mei
M
Yang
Kai
K
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019251:Nucleocapsid / Q000737:chemistry; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000821:virology*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
D012333:RNA, Messenger; D014764:Viral Proteins