title: Surface localization of the Yps3p protein of Histoplasma capsulatum.
abstract: The YPS3 gene of Histoplasma capsulatum encodes a protein that is both resident in the cell wall and also released into the culture medium. This protein is produced only during the pathogenic yeast phase of infection and is also expressed differently in H. capsulatum strains that differ in virulence. We investigated the cellular localization of Yps3p. We demonstrated that the cell wall fraction of Yps3p was surface localized in restriction fragment length polymorphism class 2 strains. We also established that Yps3p released into the G217B culture supernatant binds to the surface of strains that do not naturally express the protein. This binding was saturable and occurred within 5 min of exposure and occurred similarly with live and heat-killed H. capsulatum. Flow cytometric analysis of H. capsulatum after enzymatic treatments was consistent with Yps3p binding to chitin, a carbohydrate polymer that is a component of fungal cell walls. Polysaccharide binding assays demonstrated that chitin but not cellulose binds to and extracts Yps3p from culture supernatants.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706-1532, USA.
authors.forename: Megan L
authors.initials: ML
authors.lastname: Bohse
authors.forename: Jon P
authors.initials: JP
authors.lastname: Woods
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002473:Cell Wall / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002482:Cellulose / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D006658:Histoplasma / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006660:Histoplasmosis / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014909:Wheat Germ Agglutinins; C087923:Yps-3 protein, Ajellomyces capsulata; D002686:Chitin; D002482:Cellulose; D002688:Chitinases