Glial cell line-derived neurotrophic factor in bone marrow stromal cells of rat.
To investigate the capability of Sprague-Dawley rat bone marrow stromal cells to secrete glial cell line-derived neurotrophic factors (GDNF), we detected expression of GDNF messenger RNA and protein in bone marrow stromal cells of Sprague-Dawley rats by reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The GDNF messenger RNA and protein were detected in culture medium and total cell protein when bone marrow stromal cells were cultured for 3 days. The levels of GDNF in culture medium and total cell protein increased gradually after 3, 7 and 10 days of culture. Rat bone marrow stromal cells have the potential to secrete GDNF. Furthermore, the ability of secretion is determined by the surrounding microenvironment and self-growth status.
Ye
Min
M
Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai, Second Medical University, Shanghai 200025, China.
Chen
Shengdi
S
Wang
Xijin
X
Qi
Chen
C
Lu
Guoqiang
G
Liang
Liang
L
Xu
Jieyi
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D012333:RNA, Messenger