title: Direct detection of Nocardia spp. in clinical samples by a rapid molecular method.
abstract: We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.
authors.affiliation: UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Andrée
authors.initials: A
authors.lastname: Couble
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez-Nava
authors.forename: Michèle Pérouse
authors.initials: MP
authors.lastname: de Montclos
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D001706:Biopsy; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D009615:Nocardia / Q000235:genetics / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology; D013997:Time Factors
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S