title: [Detection and classification of Mycoplasma in cell lines].
abstract: OBJECTIVE
  To detect the rate of Mycoplasma infection in cell lines and further determine its types.
  
  
  METHODS
  We performed nest PCR amplification of Mycoplasma's conserved regions (16S-23S) and sequenced the spacer with different length between conserved regions.
  
  
  RESULTS
  Within the tested 22 cell lines, 17 (77.3%) showed Mycoplasma infections, of which 5 had two or more types of Mycoplasma. M. fermentans and hyorhinis were more frequently detected within the types of infected Mycoplasma.
  
  
  CONCLUSIONS
  The high rate of Mycoplasma infection in cell lines makes it necessary for researchers to pay more attention to its influence on research data when using cell lines as models. Establishment of detection and classifying techniques make it possible to further study the pathogenesis of different types of Mycoplasma.
authors.affiliation: Department of Genetic, Peking University School of Oncology, Beijing 100034, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.forename: Yun-wei
authors.initials: YW
authors.lastname: Lü
authors.forename: Chun-feng
authors.initials: CF
authors.lastname: Zhang
authors.forename: Bang-wei
authors.initials: BW
authors.lastname: Cao
authors.forename: Qing-bo
authors.initials: QB
authors.lastname: Chai
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Ma
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification* / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology; D016858:Mycoplasma fermentans / Q000302:isolation & purification; D045790:Mycoplasma hyorhinis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S