title: Analysis of autoantibody repertoires in small- and medium-sized vessels vasculitides. Evidence for specific perturbations in polyarteritis nodosa, microscopic polyangiitis, Churg-Strauss syndrome and Wegener's granulomatosis.
abstract: In order to identify new antibody reactivities, we have used a quantitative immunoblotting technique on extracts of normal human tissues to analyze the repertoires of serum IgM, serum IgG and purified IgG autoantibodies of patients with systemic vasculitides. Patients fulfilled the American College of Rheumatology and Chapel Hill criteria for the diagnosis of polyarteritis nodosa (PAN) (n=8), PAN related to hepatitis B virus (HBV) infection (n=5), Wegener's granulomatosis (WG) (n=6), microscopic polyangiitis (MPA) (n=18) or Churg-Strauss syndrome (CSS) (n=8). Sera from patients with chronic HBV infection without PAN (n=5) and age- and gender-matched healthy individuals (n=45) were used as controls. In the lung extract, IgM from 12/18 MPA patients reacted with high intensity with a 50 kDa band and serum IgG from 3/8 CSS patients bound to a 70 kDa protein band. In the artery extract, serum IgG from 6/18 MPA patients bound to an 85 kDa antigen, whereas purified IgG from all WG patients tested bound to a 28 kDa protein band and IgM from CSS patients bound to 2 main antigens of 38 and 60 kDa. These results provide evidence for the specificity of autoantibody repertoires from patients with PAN, WG, CSS and MPA.
authors.affiliation: UPRES EA 3408, Formation Associée Claude Bernard, UFR-SMBH-Léonard de Vinci, Bobigny, France.
authors.forename: Youri
authors.initials: Y
authors.lastname: Chanseaud
authors.forename: Mathieu C
authors.initials: MC
authors.lastname: Tamby
authors.forename: Philippe
authors.initials: P
authors.lastname: Guilpain
authors.forename: Joseph
authors.initials: J
authors.lastname: Reinbolt
authors.forename: Marianne
authors.initials: M
authors.lastname: Kambouchner
authors.forename: Nathalie
authors.initials: N
authors.lastname: Boyer
authors.forename: Laure-Hélène
authors.initials: LH
authors.lastname: Noël
authors.forename: Loïc
authors.initials: L
authors.lastname: Guillevin
authors.forename: Marie-Christophe
authors.initials: MC
authors.lastname: Boissier
authors.forename: Luc
authors.initials: L
authors.lastname: Mouthon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D016022:Case-Control Studies; D015267:Churg-Strauss Syndrome / Q000276:immunology* / Q000473:pathology; D005260:Female; D014890:Granulomatosis with Polyangiitis / Q000276:immunology* / Q000473:pathology; D006262:Health; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010488:Polyarteritis Nodosa / Q000276:immunology* / Q000473:pathology; D014657:Vasculitis / Q000276:immunology* / Q000473:pathology*
chemical_list: D000941:Antigens; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M