Serum paraoxonase 1 activity and malondialdehyde levels in patients with ulcerative colitis.
This study was designed to evaluate the oxidative and antioxidative status in patients with ulcerative colitis by detecting antioxidant enzyme paraoxonase 1 activity together with the level of a well-known marker of oxidative stress, malondialdehyde. Serum paraoxonase 1 activity and malondialdehyde levels were analysed in 30 patients with ulcerative colitis and 30 controls using a spectrophotometric method; correlation analysis was made between these variables. Serum malondialdehyde levels were higher in the ulcerative colitis group (median: 2.5, range: 0.5-9.4 nmol ml(-1)) than among the controls (median:1.1, range: 0.5-2.3 nmol ml(-1); p < 0.001) whereas paraoxonase 1 activities were lower in the ulcerative colitis group (median: 158.4, range: 61.6-264.1 U l(-1)) than in the control group (median: 233.3, range: 114.4-431.0 U l(-1); p < 0.001). There was no correlation between serum malondialdehyde level, paraoxonase 1 activity and disease activity. (1) Increased reactive oxygen metabolites levels in ulcerative colitis may result in a pro-oxidation environment, which in turn could result in decreased antioxidant paraoxonase 1 activity and increased malondialdehyde levels, (2) increased cytokines may be a possible cause of decreased paraoxonase 1 activity and (3) decreased serum paraoxonase 1 activity may be a part of an inflammatory response.
Baskol
Gulden
G
Department of Biochemistry and Clinical Biochemistry, Erciyes University School of Medicine, Kayseri, Turkey. gbaskol@yahoo.com
Baskol
Mevlut
M
Yurci
Alper
A
Ozbakir
Omer
O
Yucesoy
Mehmet
M
D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D003093:Colitis, Ulcerative / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism; D012016:Reference Values; D044967:Serum / Q000201:enzymology
D015415:Biomarkers; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D043303:Aryldialkylphosphatase