Influence of metabolic control on the blood-retinal barrier in streptozocin diabetic rats.
One hundred and eight male Sprague-Dawley rats weighing 160-225 g were examined with vitreous fluorophotometry (VF). Fifty-five rats were made diabetic with streptozocin (43 with 65 mg/kg and 12 with 90 mg/kg); 53 nondiabetic rats served as controls. Fluorescein (7 mg/kg) was injected IV and VF was performed 60 min later. The diabetic rats had an abnormal blood-retinal barrier (BRB) after 4-7 days duration. The difference between the diabetic and non-diabetic rats was persistent up to 35 days (P < 0.02-0.001). The diabetic rats treated with 90 mg/kg streptozotocin had a more severe diabetes and a higher treated with 65 mg/kg (P < 0.05). The BRB function was normalized in 18 rats treated for 6-7 days with 5-6 IU long-acting insulin (P < 0.02). The results imply that streptozocin-diabetic rats have an abnormal BRB and that this abnormality seems to be related to the diabetic state and to be reversible after insulin treatment.
Kernell
A
A
Department of Pediatrics, University Hospital, Linköping, Sweden.
Dahlkvist
H
H
Arnqvist
H
H
Ludvigsson
J
J
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001813:Blood-Retinal Barrier; D003287:Contrast Media / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D019793:Fluorescein / Q000378:metabolism; D015590:Fluorophotometry; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*; D015430:Weight Gain
D001786:Blood Glucose; D003287:Contrast Media; D007004:Hypoglycemic Agents; D007328:Insulin; D019793:Fluorescein