title: Rapid protocol for electroporation of Clostridium perfringens.
abstract: A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.
authors.affiliation: Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Jirásková
authors.forename: Libor
authors.initials: L
authors.lastname: Vítek
authors.forename: Johan
authors.initials: J
authors.lastname: Fevery
authors.forename: Tomás
authors.initials: T
authors.lastname: Ruml
authors.forename: Pavel
authors.initials: P
authors.lastname: Branny
mesh_terms: D003016:Clostridium perfringens / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*