Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices.
Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) was achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (V(max)) and Michaelis-Menten constant (K(m)) were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/solvent systems. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate on the enzyme activity and film morphology were determined. Temperature and pH optimization, operational stability and shelf-life of enzyme electrodes were also examined. Phenolic contents of green and black tea were determined by using enzyme electrodes.
Arslan
Ahu
A
Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
Kiralp
Senem
S
Toppare
Levent
L
Yagci
Yusuf
Y
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry*; D013662:Tea / Q000737:chemistry
D004800:Enzymes, Immobilized; D010636:Phenols; D011108:Polymers; D011758:Pyrroles; D012833:Siloxanes; D013662:Tea; C067635:polypyrrole; D014442:Monophenol Monooxygenase