title: Glutathione S-transferase M1, T1 and P1 polymorphisms and bladder cancer risk in Egyptians.
abstract: Previous studies suggest that bladder cancer risk may vary with GST genotype but these results are inconsistent. The aim of this study was to explore whether GSTM1, GSTT1 and GSTP polymorphisms were associated with increased bladder cancer risk in an Egyptian population. GSTM1, GSTT1 and GSTP1 genotype frequencies were determined in bladder cancer cases (n=72) and healthy controls with no history of malignancies (n=82) using PCR-based techniques. The GSTT1*2 genotype was particularly associated with increased risk (OR 2.71, 95%CI 1.27-5.73) and the GSTM1*2 genotype to a lesser extent (OR 1.63, 95%CI 0.85-3.10). 18.1% of cases but only 7.3% of controls were GSTP1*B*B homozygotes (OR 2.38, 95%CI 0.83-6.87). The presence of two or more a priori at-risk genotypes was associated with increased bladder cancer risk (OR 2.42; 95%CI 1.47-3.97). These results suggest that polymorphisms in the GST genes are associated with increased risk of bladder cancer among Egyptians.
authors.affiliation: Cancer Research UK Carcinogenesis Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.
authors.forename: A A
authors.initials: AA
authors.lastname: Saad
authors.forename: P J
authors.initials: PJ
authors.lastname: O'Connor
authors.forename: M H
authors.initials: MH
authors.lastname: Mostafa
authors.forename: N E
authors.initials: NE
authors.lastname: Metwalli
authors.forename: D P
authors.initials: DP
authors.lastname: Cooper
authors.forename: A C
authors.initials: AC
authors.lastname: Povey
authors.forename: G P
authors.initials: GP
authors.lastname: Margison
mesh_terms: D000328:Adult; D000368:Aged; D004534:Egypt; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D007527:Isoenzymes; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human