title: [Purification of chymopapain and its effect on nucleus pulposus in vitro].
abstract: OBJECTIVE
  To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.
  
  
  METHODS
  Chymopapain was purified by ion exchange chromatograph and tested its effect on nucleus pulposus in vitro.
  
  
  RESULTS
  A simple and effective procedure for purification of chymopapain was established and the chymopapain did degrade nucleus pulposus.
  
  
  CONCLUSIONS
  The ion exchange chromatograph was a simple and effective procedure for purification of chymopapain. It is necessary to study its effect on nucleus pulposus in vivo.
authors.affiliation: General Hospital of Guangzhou Military Region, Guangzhou.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Xingen
authors.initials: X
authors.lastname: Luo
authors.forename: Shujin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D031042:Asimina / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002917:Chymopapain / Q000302:isolation & purification / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000187:drug effects; D007404:Intervertebral Disc Chemolysis; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D003094:Collagen; D002917:Chymopapain