Effect of combining the larval antigens Ancylostoma secreted protein 2 (ASP-2) and metalloprotease 1 (MTP-1) in protecting hamsters against hookworm infection and disease caused by Ancylostoma ceylanicum.
Syrian Golden hamsters were vaccinated with the recombinant fusion proteins Ay-ASP-2 and Ay-MTP-1 from the infective larvae of the hookworm Ancylostoma ceylanicum. Vaccines comprised each antigen alone or the combination of the two proteins. All vaccinated group developed high antibody titers (>1:40,000); coadministration of a second antigen did not significantly affect the magnitude of the antibody response. Following challenge, hamsters vaccinated with each single antigen exhibited reductions in worm burden (32% and 28% to Ay-ASP-2 and Ay-MTP-1, respectively) and fecal egg counts (56% and 43%, respectively). A vaccine cocktail, containing both antigens further reduced worm burden (36%) and fecal egg counts (59%) (p<0.001). Moreover, vaccination with the antigen cocktail significantly improved hemoglobin values (p=0.01) and body weights (p=0.001) compared to what achieved with either each antigen or adjuvant alone. Taken together, these data suggest that combination of two or more antigens may present an effective vaccine development strategy to improve protection and/or disease symptoms in affected individuals.
Mendez
Susana
S
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, Rose Hall 736, 2300 Eye Street, NW, Washington, DC 20037, USA.
Zhan
Bin
B
Goud
Gaddam
G
Ghosh
Kashinath
K
Dobardzic
Azra
A
Wu
Wenhui
W
Liu
Sen
S
Deumic
Vehid
V
Dobardzic
Reshad
R
Liu
Yueyuan
Y
Bethony
Jeff
J
Hotez
Peter J
PJ
D000595:Amino Acid Sequence; D000722:Ancylostoma / Q000276:immunology*; D000724:Ancylostomiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology*; D001835:Body Weight; D003001:Cloning, Molecular; D006224:Cricetinae; D005243:Feces / Q000469:parasitology; D015801:Helminth Proteins / Q000276:immunology*; D006454:Hemoglobins / Q000378:metabolism; D006725:Hookworm Infections / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D007814:Larva / Q000276:immunology; D008647:Mesocricetus; D045726:Metalloproteases / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
C483968:ASP-2 protein, Ancylostoma ceylanicum; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D006454:Hemoglobins; D014614:Vaccines, Synthetic; D045726:Metalloproteases