Comparison of iron oxide labeling properties of hematopoietic progenitor cells from umbilical cord blood and from peripheral blood for subsequent in vivo tracking in a xenotransplant mouse model XXX.
OBJECTIVE
To compare and optimize ferumoxides labeling of human hematopoietic progenitor cells from umbilical cord blood and from peripheral blood for subsequent in vivo tracking with a clinical 1.5 T MR scanner.


METHODS
Human hematopoietic progenitor cells, derived from umbilical cord blood or peripheral blood, were labeled with Ferumoxides by simple incubation or lipofection. Cellular iron uptake was quantified with spectrometry. Then, 3 x 10(7)-labeled cells were injected into the tail vein of 12 female nude Balb/c mice. The mice underwent magnetic resonance imaging before and 24 hours after injection. Precontrast and postcontrast signal intensities of liver, spleen, and bone marrow were measured and tested for significant differences with the t-test. Immunostains served as a histopathologic standard of reference.


RESULTS
After labeling by simple incubation, only umbilical cord blood cells, but not peripheral blood cells, showed a significant iron uptake and could be tracked in vivo with magnetic resonance imaging. Using lipofection, both cell types could be tracked in vivo. A significant decline in signal intensity was observed in liver, spleen, and bone marrow at 24 hours after injection of efficiently labeled ferumoxides cells (P < .05). Histopathology proved the distribution of iron oxide-labeled cells to these organs.


CONCLUSIONS
Hematopoietic progenitor cells from umbilical cord blood can be labeled by simple incubation with an Food and Drug Administration-approved magnetic resonance contrast agent with sufficient efficiency to provide an in vivo cell tracking at 1.5 T. Progenitor cells from peripheral blood need to be labeled with adjunctive transfection techniques to be depicted in vivo at 1.5 T.
Daldrup-Link
Heike E
HE
Department of Radiology, University of California San Francisco, UCSF Medical Center, 513 Parnassus Avenue, San Francisco, CA 94143, USA. daldrup@radiology.ucsf.edu
Rudelius
Martina
M
Oostendorp
Robert A J
RA
Jacobs
Volker R
VR
Simon
Gerhard H
GH
Gooding
Charles
C
Rummeny
Ernst J
EJ
D000818:Animals; D002470:Cell Survival; D002498:Centrifugation; D003287:Contrast Media / Q000493:pharmacokinetics*; D003911:Dextrans; D005260:Female; D052203:Ferrosoferric Oxide; D005312:Fetal Blood / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007501:Iron / Q000493:pharmacokinetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D010087:Oxides / Q000493:pharmacokinetics; D013054:Spectrophotometry, Atomic; D013194:Staining and Labeling / Q000379:methods*; D014183:Transplantation, Heterologous
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide