An automated apparatus for the real-time monitoring of bioluminescence in plants.
We developed an automated, high-throughput, bioluminescence-monitoring apparatus that can monitor 1920 individual plant seedlings under uniform light conditions. The apparatus is composed of five units: (i) a plate platform that can hold 20 96-well microplates under uniform light conditions, (ii) a scintillation counter, (iii) a robot that conveys plates between the plate platform and a scintillation counter, (iv) a sequence controller, and (v) an external computer that collects and analyzes bioluminescence data automatically. The apparatus gave reproducible and reliable results for both bioluminescence photon counts and period length of bioluminescence rhythms; neither was affected by the well position in a plate or the plate position on the platform. The apparatus is a powerful tool for both large-scale detailed analysis of gene expression and large-scale screening of mutants.
Okamoto
Kazuhisa
K
Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
Onai
Kiyoshi
K
Ezaki
Norihiko
N
Ofuchi
Toru
T
Ishiura
Masahiro
M
D017360:Arabidopsis / Q000502:physiology; D001331:Automation; D002940:Circadian Rhythm; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D009154:Mutation; D010944:Plants / Q000235:genetics; D015203:Reproducibility of Results; D036226:Seedlings / Q000502:physiology*