Short communication: Ca2+-adenosine triphosphatase protein expression in the mammary gland of periparturient cows.
The objectives of this study were to measure the changes in protein expression of the mammary Ca2+-ATPases during the periparturient period and to determine whether Ca2+-ATPase protein expression in the mammary gland is related to milk fever (MF) development. Abundance of Ca2+-ATPase in mammary tissue and milk fat globule membranes was determined by Western blotting. The secretory pathway Ca2+-ATPase was elevated prepartum in mammary tissue from cows that developed MF compared with non-MF cows.
Prapong
S
S
Periparturient Diseases of Cattle Research Unit, USDA-ARS, National Animal Disease Center, Ames, IA 50010, USA.
Reinhardt
T A
TA
Goff
J P
JP
Horst
R L
RL
D000818:Animals; D015153:Blotting, Western; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology*; D005260:Female; D006017:Glycolipids / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D066292:Lipid Droplets; D008321:Mammary Glands, Animal / Q000201:enzymology*; D010319:Parturient Paresis / Q000201:enzymology*; D036801:Parturition; D011247:Pregnancy
D006017:Glycolipids; D006023:Glycoproteins; C110367:milk fat globule; D000252:Calcium-Transporting ATPases