Evaluation of the phospholamban gene in purebred large-breed dogs with dilated cardiomyopathy.
OBJECTIVE
To evaluate the role of the phospholamban gene in purebred large-breed dogs with dilated cardiomyopathy (DCM).


METHODS
6 dogs with DCM, including 2 Doberman Pinschers, 2 Newfoundlands, and 2 Great Danes.


METHODS
All dogs had clinical signs of congestive heart failure, and a diagnosis of DCM was made on the basis of echocardiographic findings. Blood samples were collected from each dog, and genomic DNA was isolated by a salt extraction method. Specific oligonucleotides were designed to amplify the promoter, exon 1, the 5'-part of exon 2 including the complete coding region, and part of intron 1 of the canine phospholamban gene via polymerase chain reaction procedures. These regions were screened for mutations in DNA obtained from the 6 dogs with DCM.


RESULTS
No mutations were identified in the promoter, 5' untranslated region, part of intron 1, part of the 3' untranslated region, and the complete coding region of the phospholamban gene in dogs with DCM.


CONCLUSIONS
Results indicate that mutations in the phospholamban gene are not a frequent cause of DCM in Doberman Pinschers, Newfoundlands, and Great Danes.
Stabej
Polona
P
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
Leegwater
Peter A
PA
Stokhof
Arnold A
AA
Domanjko-Petric
Aleksandra
A
van Oost
Bernard A
BA
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002311:Cardiomyopathy, Dilated / Q000235:genetics / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D004452:Echocardiography / Q000662:veterinary; D004562:Electrocardiography / Q000662:veterinary; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D017422:Sequence Analysis, DNA / Q000662:veterinary; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D009841:Oligonucleotides; D000099211:Phospholamban