title: Structure of the precursor of Xenopus laevis endothelin-3 and phylogenetic analysis.
abstract: Endothelin (ET)-related receptors homologous to mammalian receptors have been cloned from Xenopus laevis, indicating that ET-related ligands may be present in this species. Here we cloned cDNAs encoding preproendothelin-3 (PPET-3) from the X. laevis intestinal cDNA library. X. laevis ET-3 cDNA encodes 201 amino acids, including a 20-amino-acid putative signal sequence, as well as mature ET-3, big ET-3, and ET-3-like sequences. X. laevis ET-3 differs by one amino acid from mammalian ET-3, and is identical to frog ET-3 recently purified from Rana ridibunda. This sequence together with other published PPET sequences were used to analyze the phylogenetic relationship among all ET family genes. This is the first report of the cDNA encoding the precursor protein of ET-3 in a non-mammalian species.
authors.affiliation: Institute for Biological Resources and Functions, National Institute ofAdvanced Industrial Science and Technology, Japan.
authors.forename: Jiexia
authors.initials: J
authors.lastname: Quan
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takizawa
authors.forename: Javier
authors.initials: J
authors.lastname: Adur
authors.forename: Ei-ichi
authors.initials: E
authors.lastname: Nara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uchide
authors.forename: Kaname
authors.initials: K
authors.lastname: Saida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019334:Endothelin-3 / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: D019334:Endothelin-3; D029867:Xenopus Proteins