C/EBPdelta and C/EBPgamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF.
Developmental- and tissue-specific expression of globin genes is mediated by a few key elements within the proximal promoter of each gene. DNA-binding assays previously identified NF-Y, GATA-1, C/EBPbeta and C/EBPgamma as candidate regulators of beta-globin transcription via the CCAAT-box, a promoter element situated between CACC- and TATA-boxes. We have identified C/EBPdelta as an additional beta-globin CCAAT-box binding protein. In reporter assays, we show that C/EBPdelta can co-operate with EKLF, a CACC-box binding protein, to activate the beta-globin promoter, whereas C/EBPgamma inhibits the transcriptional activity of EKLF in this assay.
Gordon
Christopher T
CT
Institute for Molecular Bioscience, University of Queensland, St. Lucia, 4072, QLD, Australia.
Fox
Vanessa J
VJ
Najdovska
Suzana
S
Perkins
Andrew C
AC
D001609:Beta-Globulins / Q000096:biosynthesis*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D011485:Protein Binding / Q000502:physiology; D016385:TATA Box / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
D001609:Beta-Globulins; D004268:DNA-Binding Proteins