title: Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.
abstract: O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion.
authors.affiliation: Laboratory of Fungal Glycobiology, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Perlińska-Lenart
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kurzatkowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Janas
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kopińska
authors.forename: Grazyna
authors.initials: G
authors.lastname: Palamarczyk
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Kruszewska
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001483:Base Sequence; D017931:DNA Primers; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006031:Glycosylation; D046529:Microscopy, Electron, Transmission; D009154:Mutation
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins