[Experimental study of human skin fibroblasts cultured in three-dimension(3D)].
OBJECTIVE
To investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL).


METHODS
The human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology.


RESULTS
The flow cytometry showed that the G0/G1, stage cells were 79% +/- 3%, 87% +/- 2% after the 7 days and 14 days separately, and there were not apoptosis peak observed. RT-PCR analysis revealed that the mRNA expression of TGF beta1, and fibronectin had no difference between human skin fibroblasts cultured in 3D and 2D. Electron microscope showed the cells were plenty of chromatin and organelles.


CONCLUSIONS
The proliferation of the human skin fibroblasts in FPCL is slow, but its biological viability is better.
Liu
Zhi-guo
ZG
Center for Special Medicine, Hospital of PLA, Beijing, China.
Huan
Jing-ning
JN
Chen
Yu-lin
YL
Ge
Sheng-de
SD
Fang
Zhi-yang
ZY
Ouyang
Tian-xiang
TX
Xing
Xin
X
D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D051381:Rats; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
D003094:Collagen