[Population studies of Exophiala jeanselmeis by sequence analysis in the ribosomal DNA].
OBJECTIVE
To study the populations of Exophiala jeanselmei strains from diverse sources using rDNA sequences.


METHODS
Tested strains included 20 isolates of Exophiala jeanselmei (type culture, reference culture, clinical and environmental isolates), 8 isolates of E. moniliae, 1 isolate of Exophiala werneckii, respectively. DNA was isolated with boiled-iced water method. Ribosomal DNA and its internal transcribed spacer regions were amplified and analyzed with sequencing.


RESULTS
The ribosomal genes of E. jeanselmei varied greatly. Twenty strains of this species were divided into 7 types. The genetic distances of some strains in this species were quite long.


CONCLUSIONS
The ribosomal RNA genes and their internal transcript sequences vary more widely than those of the other species in this genus.
Li
Dong-ming
DM
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Li
Ruo-yu
RY
Wang
Xiao-hong
XH
Wan
Zhe
Z
Ma
Sheng-qing
SQ
Wang
Duan-li
DL
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005093:Exophiala / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques / Q000379:methods*; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal