title: Simple enumerations of peripheral blood natural killer (CD56+ NK) cells, B cells and T cells have no predictive value in IVF treatment outcome.
abstract: BACKGROUND
  To evaluate the association between the absolute counts of the peripheral natural killer (NK) cells (including total CD56(+) NK cells, CD56(dim) NK cells and CD56(bright) NK cells), B cells and T cells on the implantation rate and miscarriage rate after IVF treatment.
  
  
  METHODS
  This was a prospective observation study. A total of 138 patients who underwent IVF treatment from December 2002 to July 2003 were recruited to the study. Blood samples were obtained on the day of vaginal oocyte retrieval prior to the procedure. The absolute counts of lymphocytes, NK cells, B cells and T cells were identified by flow cytometry. These absolute counts and their relationships to IVF treatment outcome and miscarriage rate were analysed.
  
  
  RESULTS
  There were no significant differences with regard the mean values of absolute lymphocyte count, T cell count, B cell count and NK cell count (including total CD56(+) NK, CD56(dim) NK and CD56(bright) NK cells) between the pregnant and non-pregnant groups and also between the ongoing pregnancy and miscarriage groups. The cause of infertility, duration of infertility, basal FSH levels, number of previous failed IVF treatments, number of previous miscarriages and stimulation characteristics were not significantly different between the pregnant and non-pregnant groups. Previous studies have suggested that women with a history of recurrent miscarriage and those with infertility accompanied by recurrent failed IVF treatments are associated with a peripheral blood NK cell percentage >12%, therefore further analysis of peripheral CD56(+) NK cell levels <12% (group A) and >12% (group B) was performed. There was no significant difference in implantation rate (group A: 17.0%; group B: 23.2%), pregnancy rate (group A: 36.6%; group B: 47.7%) or miscarriage rate (group A: 23.3%; group B: 28.6%).
  
  
  CONCLUSIONS
  There were no significant differences between simple enumerations of peripheral blood NK cells (including total CD56(+) NK, CD56(dim) NK and CD56(bright) NK cells), B cells and T cells with IVF treatment outcome and pregnancy outcome. Women who had a peripheral NK cell level >12% did not have higher number of previous pregnancy losses. Importantly their pregnancy rate was not reduced and their miscarriages were not increased compared to women who had a peripheral NK cells level <12%.
authors.affiliation: Lister Fertility Clinic, Lister Hospital, London, UK. mythum@doctors.net.uk
authors.forename: M Y
authors.initials: MY
authors.lastname: Thum
authors.forename: S
authors.initials: S
authors.lastname: Bhaskaran
authors.forename: A S
authors.initials: AS
authors.lastname: Bansal
authors.forename: H
authors.initials: H
authors.lastname: Shehata
authors.forename: B
authors.initials: B
authors.lastname: Ford
authors.forename: N
authors.initials: N
authors.lastname: Sumar
authors.forename: H I
authors.initials: HI
authors.lastname: Abdalla
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology; D000328:Adult; D001402:B-Lymphocytes / Q000502:physiology*; D019002:CD56 Antigen; D010064:Embryo Implantation / Q000276:immunology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011446:Prospective Studies; D013601:T-Lymphocytes / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: D019002:CD56 Antigen