Induction of Gag-specific T-cell responses by therapeutic immunization with a Gag-expressing Sendai virus vector in macaques chronically infected with simian-human immunodeficiency virus.
Recent prophylactic vaccine trials inducing virus-specific CD8+ T-cell responses have shown control of primary infections of a pathogenic simian-human immunodeficiency virus (SHIV) in macaques. In the chronic phase, therapeutic immunization replenishing virus-specific CD8+ T-cells is likely to contribute to sustained control of virus replication. In this study, we have administered a recombinant Sendai virus (SeV) vector into five rhesus macaques that had received prophylactic vaccinations and had controlled SHIV replication for more than 1 year after challenge. Our results indicate that virus-specific CD8+ T-cell responses can be expanded and broadened by therapeutic immunization with SeV vectors in the chronic phase after prophylactic vaccine-based control of primary immunodeficiency virus infections.
Kato
Moriaki
M
Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Igarashi
Hiroko
H
Takeda
Akiko
A
Sasaki
Yuri
Y
Nakamura
Hiromi
H
Kano
Munehide
M
Sata
Tetsutaro
T
Iida
Akihiro
A
Hasegawa
Mamoru
M
Horie
Shigeo
S
Higashihara
Eiji
E
Nagai
Yoshiyuki
Y
Matano
Tetsuro
T
D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000276:immunology; D015683:Gene Products, gag / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D018655:Lymphocyte Count; D008253:Macaca mulatta; D008297:Male; D009000:Monocytes / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D029082:Sendai virus / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology*; D019562:Viral Load
D015683:Gene Products, gag; D012367:RNA, Viral; D004247:DNA