title: The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations.
abstract: The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Kershaw
authors.forename: Nigel L
authors.initials: NL
authors.lastname: Brown
authors.forename: Chrystala
authors.initials: C
authors.lastname: Constantinidou
authors.forename: Mala D
authors.initials: MD
authors.lastname: Patel
authors.forename: Jon L
authors.initials: JL
authors.lastname: Hobman
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D005798:Genes, Bacterial / Q000187:drug effects; D006706:Homeostasis / Q000235:genetics; D007501:Iron / Q000378:metabolism; D016296:Mutagenesis; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D018085:Regulon; D013481:Superoxides / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D013481:Superoxides; C085504:CpxR protein, Bacteria; D003300:Copper; D007501:Iron; D011494:Protein Kinases; C490475:CpxA protein, E coli; D015032:Zinc