Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells.
Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
von Messling
Veronika
V
Molecular Medicine Program, Mayo Clinic, Guggenheim 1838, 200 1st Street SW, Rochester, MN 55905, USA.
Oezguen
Numan
N
Zheng
Qi
Q
Vongpunsawad
Sompong
S
Braun
Werner
W
Cattaneo
Roberto
R
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D002478:Cells, Cultured; D004217:Distemper Virus, Canine / Q000502:physiology*; D005289:Ferrets; D006023:Glycoproteins; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology*; D007136:Immunoglobulins; D007963:Leukocytes, Mononuclear / Q000821:virology; D008561:Membrane Fusion; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014779:Virus Replication
D015703:Antigens, CD; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1