title: Proteomic profiling identifies an UV-induced activation of cofilin-1 and destrin in human epidermis.
abstract: The human skin is the only line of defense against UV radiation. A series of responses to protect the skin are induced by UV radiation. In this study, a proteomic approach was used to study these responses. We have performed high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of (solar simulated) UV-exposed reconstructed skin equivalents as well as native skin. Differentially expressed proteins were processed for mass spectrometric analysis, when consistent differences were observed in all individual human skin equivalents. In addition to proteins known to be involved in UV responses (HSP27, MnSOD, and PDX-2), we identified two novel proteins that were downregulated following UV exposure. Further analysis revealed that these proteins were the phosphorylated forms of the actin cytoskeleton modulators cofilin-1 and destrin. The de-phosphorylation of cofilin-1 was confirmed using western blotting of UV-exposed skin equivalents and ex vivo skin protein extracts. In conclusion, our study indicates the potency of a proteomic approach to study UV-induced changes in a tissue culture system mimicking human skin as well as excised human skin.
authors.affiliation: Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Paulj
authors.initials: P
authors.lastname: Hensbergen
authors.forename: Astride
authors.initials: A
authors.lastname: Alewijnse
authors.forename: Johanna
authors.initials: J
authors.lastname: Kempenaar
authors.forename: Roel C
authors.initials: RC
authors.lastname: van der Schors
authors.forename: Rosec
authors.initials: R
authors.lastname: van der Schors
authors.forename: Crinai A
authors.initials: CA
authors.lastname: Balog
authors.forename: Andrem
authors.initials: A
authors.lastname: Deelder
authors.forename: Gerrit
authors.initials: G
authors.lastname: Beumer
authors.forename: Maria
authors.initials: M
authors.lastname: Ponec
authors.forename: Cornelis P
authors.initials: CP
authors.lastname: Tensen
mesh_terms: D051339:Actin Depolymerizing Factors; D015153:Blotting, Western; D051345:Destrin; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000502:physiology* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation / Q000528:radiation effects; D040901:Proteomics; D013472:Sunlight; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D051339:Actin Depolymerizing Factors; C495824:DSTN protein, human; D051345:Destrin; D008840:Microfilament Proteins