title: Novel tumor necrosis factor-knockout mice that lack Peyer's patches.
abstract: We generated a novel tumor necrosis factor (TNF) null mutation using Cre-loxP technology. Mice homozygous for this mutation differ from their "conventional" counterparts; in particular, they completely lack Peyer's patches (PP) but retain all lymph nodes. Our analysis of these novel TNF-knockout mice supports the previously disputed notion of the involvement of TNF-TNFR1 signaling in PP organogenesis. Availability of TNF-knockout strains both with and without PP enables more definitive studies concerning the roles of TNF and PP in various immune functions and disease conditions. Here, we report that systemic ablation of TNF, but not the presence of PP per se, is critical for protection against intestinal Listeria infection in mice.
authors.affiliation: Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, USA. kuprash@online.ru
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Kuprash
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Tumanov
authors.forename: Dmitry J
authors.initials: DJ
authors.lastname: Liepinsh
authors.forename: Ekaterina P
authors.initials: EP
authors.lastname: Koroleva
authors.forename: Marina S
authors.initials: MS
authors.lastname: Drutskaya
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Kruglov
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shakhov
authors.forename: Eileen
authors.initials: E
authors.lastname: Southon
authors.forename: William J
authors.initials: WJ
authors.lastname: Murphy
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Grivennikov
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Nedospasov
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000276:immunology; D007150:Immunohistochemistry; D008088:Listeriosis / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics / Q000276:immunology*; D009154:Mutation; D010581:Peyer's Patches / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha