Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
Easterday
W Ryan
WR
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.
Van Ert
Matthew N
MN
Simonson
Tatum S
TS
Wagner
David M
DM
Kenefic
Leo J
LJ
Allender
Christopher J
CJ
Keim
Paul
P
D000881:Anthrax / Q000175:diagnosis* / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D018389:Codon, Nonsense; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019914:Taq Polymerase / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004269:DNA, Bacterial; C099480:PlcR protein, Bacillus; D015534:Trans-Activators; D019914:Taq Polymerase