In vivo absorption spectroscopy of tumor sensitizers with femtosecond white light.
A system based on a femtosecond white-light continuum and a streak camera was used for recordings of the in vivo absorption spectra of the tumor-seeking agent disulphonated aluminum phthalocyanine. Measurements for different drug doses were performed on tumor tissue (muscle-implanted adenocarcinoma) and normal muscle tissue in rats. It was found that the shape of the spectrum is tissue dependent. The peak of the absorption spectrum is blueshifted in tumor tissue as compared with the muscle. Thus the contrast in the drug-related absorption can be altered by up to a factor of 2 from the primary drug molecular-concentration contrast between normal muscle and tumor by the proper selection of the illumination wavelength.
af Klinteberg
Claes
C
Department of Physics, Lund Institute of Technology, Lund S-221 00, Sweden.
Pifferi
Antonio
A
Andersson-Engels
Stefan
S
Cubeddu
Rinaldo
R
Svanberg
Sune
S
D000042:Absorption; D000818:Animals; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D007211:Indoles / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008027:Light; D009942:Organometallic Compounds / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use; D010778:Photochemotherapy / Q000379:methods; D011838:Radiation-Sensitizing Agents / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D051381:Rats; D011920:Rats, Inbred WF; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C060570:aluminum phthalocyanine