title: Human leukocyte antigen expression by peritoneal macrophages from women with pelvic endometriosis is depressed but coordinated with costimulatory molecule expression.
abstract: OBJECTIVE
  To investigate the macrophage response in endometriosis, we determined expression of human leukocyte antigen (HLA)-ABC, HLA-DR, and their costimulatory molecules by peritoneal fluid (PF) macrophages.
  
  
  METHODS
  Case-control study of immunologic markers.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  We compared 38 Japanese women with endometriosis with 59 control subjects who were given other laparoscopic diagnoses.
  
  
  METHODS
  Venipuncture and laparoscopic peritoneal fluid collection.
  
  
  METHODS
  Expression of HLA-ABC, HLA-DR, CD54, CD40, CD58, CD80, and CD86 by peripheral blood (PB) monocytes and PF macrophages was quantitated as mean fluorescence intensities by flow cytometry. Expression of each marker on PF macrophages was divided by that on PB monocytes as an index of macrophage activation (macrophage activation ratio).
  
  
  RESULTS
  In women with endometriosis, PF macrophages showed significant positive correlations between expression of HLA-ABC and other costimulatory molecules and also between HLA-DR and their costimulatory molecules. However, expression of HLA-ABC and DR by PF macrophages, and also their activation ratios, were significantly lower than in controls.
  
  
  CONCLUSIONS
  Coordination with costimulatory molecules but relatively low expression of HLA-ABC and HLA-DR indicates a positive but limited immune response (antigen presentation) to events in the peritoneal cavity in women with endometriosis. This may induce immune tolerance to implanted or metaplastic endometrial tissue.
authors.affiliation: Department of Obstetrics and Gynecology, Kochi Medical School, Kohasu, Oko, Nankoku, Kochi 783-8505, Japan. kusumet@pop07.odn.ne.jp
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kusume
authors.forename: Nagamasa
authors.initials: N
authors.lastname: Maeda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Izumiya
authors.forename: Yorito
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Hayashi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Oguri
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nishimori
authors.forename: Takao
authors.initials: T
authors.lastname: Fukaya
mesh_terms: D000328:Adult; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D015415:Biomarkers / Q000378:metabolism*; D019013:CD40 Antigens / Q000378:metabolism; D018968:CD58 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000378:metabolism*; D015234:HLA-A Antigens / Q000378:metabolism; D015235:HLA-B Antigens / Q000378:metabolism; D015236:HLA-C Antigens / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens; D018968:CD58 Antigens; C497964:CD86 protein, human; D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1