title: New tool for metabolic pathway engineering in Escherichia coli: one-step method to modulate expression of chromosomal genes.
abstract: A simple and highly efficient method was developed to produce a library of Escherichia coli clones that express a particular chromosomal gene at a wide range of expression levels. The basic strategy was to replace all or part of the upstream region of a coding sequence containing the elements involved in its expression (promoter, operator, gene coding for a regulator, ribosome binding site, and start codon) with a PCR-generated library of expression cassettes.
authors.affiliation: CRITT-Bioindustries CRT, INSA, DGBA, Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Meynial-Salles
authors.forename: Marguerite A
authors.initials: MA
authors.lastname: Cervin
authors.forename: Philippe
authors.initials: P
authors.lastname: Soucaille
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic
chemical_list: D029968:Escherichia coli Proteins