title: The antiprogesterone Org 31710 inhibits human blastocyst-endometrial interactions in vitro.
abstract: OBJECTIVE
  To investigate the effect of the anti-P Org 31710 on human blastocyst attachment to cultured endometrial epithelial cells.
  
  
  METHODS
  Experimental in vitro study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Eleven fertile endometrial donors.
  
  
  METHODS
  Timed endometrial biopsy for cell cultures.
  
  
  METHODS
  Blastocyst attachment rate on endometrial cell cultures; secretion of glycodelin and leukemia inhibitory factor into the culture medium measured by RIA and ELISA techniques; and expression of progesterone receptors, interleukin-1 receptor type-1, and integrin subunit beta(3) on endometrial epithelial cells examined by immunohistochemistry. Endometrial pinopodes visualized by scanning electron microscopy.
  
  
  RESULTS
  Eleven of 16 human blastocysts attached to control cultures, whereas none of 10 blastocysts attached when Org 31710 was added to the culture medium (P=.0007). Immunohistochemical studies demonstrated no significant differences between groups. Biochemical analyses displayed a trend toward higher glycodelin secretions and, by scanning electron microscopy, a tendency toward less pinopode formation in the Org 31710 group, but the results did not reach statistical significance. The presence of swollen microvilli, precursors of endometrial pinopodes, was significantly reduced on cultures with Org 31710 (P=.03).
  
  
  CONCLUSIONS
  The study presents a model for human blastocyst-endometrial interactions responding to an anti-P drug. The exact mechanism for the anti-attachment properties of Org 31710 on the cultured endometrial cells and the blastocysts needs further evaluations.
authors.affiliation: Department of Obstetrics and Gynecology, Herlev University Hospital, Herlev Ringvej 75, DK-2730 Herlev, Denmark. astrid.petersen@dadlnet.dk
authors.forename: Astrid
authors.initials: A
authors.lastname: Petersen
authors.forename: Ursula
authors.initials: U
authors.lastname: Bentin-Ley
authors.forename: Vibeke
authors.initials: V
authors.lastname: Ravn
authors.forename: Klaus
authors.initials: K
authors.lastname: Qvortrup
authors.forename: Steen
authors.initials: S
authors.lastname: Sørensen
authors.forename: Henrik
authors.initials: H
authors.lastname: Islin
authors.forename: Anita
authors.initials: A
authors.lastname: Sjögren
authors.forename: Sietse
authors.initials: S
authors.lastname: Mosselmann
authors.forename: Lars
authors.initials: L
authors.lastname: Hamberger
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D004963:Estrenes / Q000494:pharmacology*; D005260:Female; D005663:Furans / Q000494:pharmacology*; D000071816:Glycodelin; D006023:Glycoproteins / Q000378:metabolism; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D039661:Integrin beta3 / Q000378:metabolism; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008855:Microscopy, Electron, Scanning; D011257:Pregnancy Proteins / Q000378:metabolism; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D053573:Receptors, Interleukin-1 Type I; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D003470:Culture Media; D004963:Estrenes; D005663:Furans; D000071816:Glycodelin; D006023:Glycoproteins; D006727:Hormone Antagonists; D039661:Integrin beta3; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D011506:Proteins; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D011980:Receptors, Progesterone; C076196:Org 31710; D011374:Progesterone