Co-transfection of messenger RNA and siRNA as a method to study the efficiency of siRNA.
The definition of an optimal siRNA results from the in vitro testing of several siRNA designed to specifically target a gene. Usually, such in vitro tests consist in the transfection of the several siRNA duplexes in a cell expressing stably the gene of interest. When a siRNA specific for a mRNA coding toxic proteins (certain transcription factors, transporters, toxins, cell cycle controlling proteins, etc.) must be tested, the generation of a target cell is difficult. Here we report a quick method to test the efficiency of a siRNA through its co-transfection with the targeted mRNA. This technique can be used as a fast method to test siRNA even when they target genes that cannot be stably expressed in the cells of interest.
Mutzke
Thorsten
T
CureVac GmbH, Tübingen, Germany.
Schubkegel
Gudrun
G
Teufel
Regina
R
Ketterer
Thomas
T
Probst
Jochen
J
Scheel
Birgit
B
Carralot
Jean-Philippe
JP
Pascolo
Steve
S
Ghoreschi
Kamran
K
Weigert
Christina
C
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells; D018274:Electroporation; D015870:Gene Expression; D051379:Mice; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering