Prolonged survival of donor-specific rat intestinal allograft by administration of bone-marrow-derived immature dendritic cells.
It has been reported that intraportal administration of donor antigens induced donor-specific hyporesponsiveness. We studied here the effects of transplantation of BM-derived immature dendritic cells (imDCs) and mature DCs (mDCs) via portal vein on rat small intestinal allograft survival. This study comprised four treatment groups: 1) untreated controls; 2) FK506 alone; 3) intraportal donor-specific BM-derived imDCs transplantation+FK506; 4) mDCs/Tx+FK506. Allograft survival was minimal in control group (5.2+/-0.8 days) and maximal in imDC+FK506 group (28.4+/-3.0 days). The rats in mDC+FK506 group showed systemic inflammatory reaction due to GVHR, and died within 10 days after transplantation. The in vitro MLR reaction using imDCs was also strongly inhibited both in direct and indirect recognition pathways. The impact of imDCs for the specific induction of transplant tolerance may suggest that immunization with donor-specific imDCs has therapeutic potential in organ transplantation.
Sheng Sun
Dong
D
Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata, Okayama 700-8558, Japan.
Iwagaki
Hiromi
H
Ozaki
Michitaka
M
Ogino
Tetsuya
T
Kusaka
Satoshi
S
Fujimoto
Yoshimi
Y
Murata
Hiroshi
H
Sadamori
Hiroshi
H
Matsukawa
Hiroyoshi
H
Tanaka
Noriaki
N
Yagi
Takahito
T
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000276:immunology / Q000637:transplantation*; D006084:Graft Rejection / Q000517:prevention & control*; D006085:Graft Survival / Q000276:immunology*; D007421:Intestine, Small / Q000166:cytology* / Q000473:pathology; D051381:Rats; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous