title: Evaluation of developmental changes in the coexpression of myosin heavy chains and metabolic properties of equine skeletal muscle fibers.
abstract: OBJECTIVE
  To determine the growth-related changes in metabolic and anatomic properties in equine muscle fiber type, including hybrid fibers identified with immunohistochemical analysis.
  
  
  METHODS
  24 2-, 6-, 12-, and 24-month-old female Thoroughbreds.
  
  
  METHODS
  Samples were obtained from the gluteus medius muscle of all horses. Expression of myosin heavy chain (MHC) isoforms MHC-I, -IIa, -IIb, and -IIx in each muscle fiber was detected by use of 4 primary monoclonal antibodies: BA-D5, SC-71, BF-F3, and BF-35, respectively. Five muscle fiber types (types I, I/IIA, IIA, IIA/IIX, and IIX) were immunohistochemically identified. The area and activity of succinic dehydrogenase (SDH) in each fiber type were determined by use of quantitative histochemical staining and image analysis.
  
  
  RESULTS
  Although the proportion of type I and IIX fibers did not change with age, the proportion of type IIA and IIA/IIX fibers significantly increased and decreased, respectively, from 2 months to 24 months of age. The increase in proportion of type IIA fibers with growth may have been attributable to muscle fiber-type transition from type IIA/IIX fibers but not from type IIX fibers. Values for SDH activity and fiber area in hybrid fiber types were intermediate to those for their respective pure phenotypes.
  
  
  CONCLUSIONS
  Hybrid fibers have an important role for determining the proportion of muscle fiber type in horses < 24 months old, and the metabolic and anatomic properties of the hybrid fibers are well coordinated, as in mature horses.
authors.affiliation: Department of Biological Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515 Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Yamano
authors.forename: Daisuke
authors.initials: D
authors.lastname: Eto
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kasashima
authors.forename: Atushi
authors.initials: A
authors.lastname: Hiraga
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyata
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D004586:Electrophoresis / Q000662:veterinary; D005260:Female; D006736:Horses / Q000254:growth & development / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000662:veterinary; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D020033:Protein Isoforms / Q000378:metabolism; D013385:Succinate Dehydrogenase
chemical_list: D000911:Antibodies, Monoclonal; D020033:Protein Isoforms; D009580:Nitroblue Tetrazolium; D013385:Succinate Dehydrogenase; D018995:Myosin Heavy Chains