title: Expression profile of osteoblast lineage at defined stages of differentiation.
abstract: The inherent heterogeneity of bone cells complicates the interpretation of microarray studies designed to identify genes highly associated with osteoblast differentiation. To overcome this problem, we have utilized Col1a1 promoter-green fluorescent protein transgenic mouse lines to isolate bone cells at distinct stages of osteoprogenitor maturation. Comparison of gene expression patterns from unsorted or isolated sorted bone cell populations at days 7 and 17 of calvarial cultures revealed an increased specificity regarding which genes are selectively expressed in a subset of bone cell types during differentiation. Furthermore, distinctly different patterns of gene expression associated with major signaling pathways (Igf1, Bmp, and Wnt) were observed at different levels of maturation. Some of our data differ from current models of osteoprogenitor cell differentiation and emphasize components of the pathways that were not revealed in studies based on a total cell population. Thus, applying methods to generate more homogeneous populations of cells at a defined level of cellular differentiation from a primary osteogenic culture is feasible and leads to a novel interpretation of the gene expression associated with increasing levels of osteoprogenitor maturation.
authors.affiliation: Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06053, USA.
authors.forename: Ivo
authors.initials: I
authors.lastname: Kalajzic
authors.forename: Ada
authors.initials: A
authors.lastname: Staal
authors.forename: Wen-Pin
authors.initials: WP
authors.lastname: Yang
authors.forename: Yuli
authors.initials: Y
authors.lastname: Wu
authors.forename: Susan E
authors.initials: SE
authors.lastname: Johnson
authors.forename: Jean H M
authors.initials: JH
authors.lastname: Feyen
authors.forename: Winfried
authors.initials: W
authors.lastname: Krueger
authors.forename: Peter
authors.initials: P
authors.lastname: Maye
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Lynn
authors.initials: L
authors.lastname: Kuo
authors.forename: Rishi R
authors.initials: RR
authors.lastname: Gupta
authors.forename: Luke E K
authors.initials: LE
authors.lastname: Achenie
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Dong-Guk
authors.initials: DG
authors.lastname: Shin
authors.forename: David W
authors.initials: DW
authors.lastname: Rowe
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D012313:RNA