title: Synthesis and bioactivities of two multiple antigen peptides as potential vaccine against schistosoma.
abstract: Based on the two antigenic peptides, 26-43 (P26) and 116-131 (P116), derived from 28 kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST), two multiple antigenic peptides (MAPs), (P26)4-MAP and (P116)4-MAP with the same oligomeric lysine core, were synthesized by stepwise solid-phase peptide synthesis method. The antigenicities and protective effects of these two MAPs were examined on experimental animals. As shown in the dot-ELISA result, the synthetic MAPs could be recognized and bound by immunoglobins in both patient's and infected-rabbit's sera. After Kunming mice were immunized with (P26)4-MAP, the worm burden reduction rate and the liver egg reduction rate were 59.9% and 61.1%. In (P26)4-MAP or (P116)4-MAP immunized BALB/c mice, the worm burden reduction rates were 37.5% and 62.5%, respectively, and the liver egg reduction rates were 35.1% and 54.0%, respectively.
authors.affiliation: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China.
authors.forename: He-Qing
authors.initials: HQ
authors.lastname: Huang
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Li
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Qin
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Cao
authors.forename: Yun
authors.initials: Y
authors.lastname: Yao
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Liu
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Li
authors.forename: Meng-Shen
authors.initials: MS
authors.lastname: Cai
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: You-En
authors.initials: YE
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000201:enzymology / Q000382:microbiology; D012555:Schistosomiasis mansoni / Q000276:immunology*; D014612:Vaccines
chemical_list: D000947:Antigens, Helminth; D010455:Peptides; D014612:Vaccines; D005982:Glutathione Transferase