Early dynamics of hepatitis C virus in the circulation of chimpanzees with experimental infection.
Two chimpanzees were inoculated with hepatitis C virus (HCV) and followed on a daily basis for 12 days. HCV RNA became detectable in their sera on day 5 by polymerase chain reaction with the detection limit of 10(2) copies/ml. Based on an exponential growth observed until 8 or 9 days after inoculation in their sera, the doubling time of HCV in the circulation was estimated at 6.3-8.6 h and log time (time required to grow 10-fold) at 31.3- 42.9 h. The exact doubling time of HCV determined in them would help plan an efficient strategy for screening out blood donors in the window period of infection between the exposure and the development of antibody to HCV in serum.
Tanaka
Junko
J
Department of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Katayama
Keiko
K
Kumagai
Junko
J
Komiya
Yutaka
Y
Yugi
Hisao
H
Kishimoto
Shinya
S
Mizui
Masaaki
M
Tomoguri
Tetsushi
T
Miyakawa
Yuzo
Y
Yoshizawa
Hiroshi
H
D000818:Animals; D004195:Disease Models, Animal; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000821:virology*; D007700:Kinetics; D002679:Pan troglodytes; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014766:Viremia
D012367:RNA, Viral