[Distribution of Orexin-A mRNA expression in different organs and its variation in acute inflammation].
OBJECTIVE
To explore the distribution of Orexin-A mRNA expression in different organs, and its variation in acute inflammation, and to evaluate the role of Orexin-A in acute inflammatory responses.


METHODS
Hypothalamus, adipose tissue, lung, stomach, duodenum, liver, spleen and testis of male Sprague-Dawley rats (including normal and those of intestinal ischemia/reperfusion injury model) were harvested, and reverse transcription-polymerase chain reaction(RT-PCR) was used to assess Orexin-A mRNA expression in different tissues of normal rats, and that in the hypothalamus of ischemia/reperfusion injured rats.


RESULTS
All tissues expressed Orexin-A mRNA, and it was especially high in the stomach, lung and kidney. After the acute stimulus of ischemia/reperfusion injury, Orexin-A mRNA expression showed a trend of fluctuant declination in the hypothalamus.


CONCLUSIONS
Orexin-A mRNA is widely expressed in the major organs, and it has a time-dependent response to acute inflammatory stimuli. The results suggest that its expression variation may be used as a novel marker for acute inflammation.
Yan
Guang-Tao
GT
Research Laboratory of Biochemistry, General Hospital of PLA, Beijing 100853, China. yan301@263.net
Lin
Ji
J
Liao
Jie
J
D000818:Animals; D004195:Disease Models, Animal; D007031:Hypothalamus / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D000068797:Orexins; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D014781:Viscera / Q000378:metabolism*
D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D012333:RNA, Messenger