Antibiotic produced by Fusarium equiseti NRRL 5537.
Fusarium equiseti NRRL 5537 grown on an autoclaved white corn grit medium for 3 to 4 weeks at room temperature produced a substance in excess of 5 g/kg of substrate that inhibited some gram-positive bacteria including mycobacteria. Most Bacillus subtilis, Mycobacterium phlei, and Staphylococcus aureus strains were inhibited when 1 mug of the antibiotic per ml was incorporated into the culture medium. Except for Neisseria perflava, gram-negative bacteria, yeasts, and molds were not inhibited by 128 mug/ml. The antibiotic was recovered as a white powder, had a melting point of 65 to 66 C, and had an intraperitoneal mean lethal dose in white mice of 63 mg/kg of body weight. In thin-layer chromatographic analysis the compound appeared as a single spot in two different solvent systems. Mass spectrometry determined that the molecular weight of the antibiotic was 373 with a molecular formula of C(22)H(31)NO(4). Chemical microanalysis was in accord with the formula.
Burmeister
H R
HR
Bennett
G A
GA
Vesonder
R F
RF
Hesseltine
C W
CW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D005260:Female; D005670:Fusarium / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D011760:Pyrrolidinones / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D011760:Pyrrolidinones