title: Effects of hyperbaric oxygen exposure on experimental head and neck tumor growth, oxygenation, and vasculature.
abstract: BACKGROUND
  Hyperbaric oxygen (HBO2) is used to promote healing in irradiated tissues, but concern persists about the possibility that it may promote residual tumor growth.
  
  
  METHODS
  The tumor growth of SQ20B and Detroit 562 head and neck squamous cell carcinoma xenografts were studied after single-dose irradiation and 5x/week HBO2 treatment at 2.4 atm absolute for 90 minutes. The effect of HBO2 treatment on tumor hypoxia and vasculature was also examined by immunohistochemical analysis.
  
  
  RESULTS
  HBO2 treatment increased tumor oxygenation during the treatment interval but did not promote the growth of either irradiated or unirradiated tumors. No increase in tumor vascular endothelial growth factor expression or vascularization was detected.
  
  
  CONCLUSIONS
  This study found no evidence for persistent changes in tumor microenvironment or tumor growth promotion caused by hyperbaric oxygen exposure.
authors.affiliation: Department of Radiation Oncology, University of Pennsylvania, 195 John Morgan Building, 37th and Hamilton Walk, Philadelphia, PA 19104-6072, USA.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Shi
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Lee
authors.forename: Junmin
authors.initials: J
authors.lastname: Wu
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Koch
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Thom
authors.forename: Amit
authors.initials: A
authors.lastname: Maity
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Bernhard
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D015687:Cell Hypoxia; D004307:Dose-Response Relationship, Radiation; D006258:Head and Neck Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D006931:Hyperbaric Oxygenation; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A