title: Activation of oxidative burst and degranulation of porcine neutrophils by a homologous spleen galectin-1 compared to N-formyl-L-methionyl-L-leucyl-L-phenylalanine and phorbol 12-myristate 13-acetate.
abstract: Galectins are a family of animal lectins defined by their beta-galactoside-binding activities and a consensus sequence in their carbohydrate-recognizing domain (CRD). Relevant roles of galectins are described in adaptive immune response, innate immunity and modulation of the acute inflammatory response. We have extended our previous studies on a porcine spleen galectin-1 in relation to its functional roles such as polymorphonuclear neutrophils (PMNs) stimulation compared to well known PMN activators e.g. N-formyl-L-methionyl-L leucyl-L-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA). Relative to activation of NADPH-oxidase fMLP and PMA are stronger than galectin-1 plus cytochalasin B (CB) when the lectin is employed at low concentrations (gal-1 1 microM, 3.6+0.8 nm O(2)(-)/min/10(7) PMN). Higher doses of galectin-1 (10 microM) plus CB produced a significant activation of NADPH-oxidase (27.9+14.8 nm O(2)(-)/min/10(7) PMN) and stimulated PMN degranulation up to 50%. We propose that local galectin-1 concentrations under physiological conditions might reach suitable levels for pig PMN stimulation, and might be a natural inducer of O(2)(-) formation or degranulation. Porcine galectins might produce enhanced responses in vivo when they stimulate neutrophils in combination with some other stimuli.
authors.affiliation: Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, 1900 La Plata, Argentina.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Elola
authors.forename: María Elena
authors.initials: ME
authors.lastname: Chiesa
authors.forename: Nilda Ester
authors.initials: NE
authors.lastname: Fink
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D015550:Cell Degranulation / Q000187:drug effects; D037483:Galectin 1 / Q000302:isolation & purification / Q000494:pharmacology* / Q000502:physiology; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D028321:Plant Preparations / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014118:Toxins, Biological / Q000494:pharmacology
chemical_list: D037483:Galectin 1; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488865:VAA-I protein, Viscum album; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019255:NADPH Oxidases; D009113:Muramidase; D054788:Ribosome Inactivating Proteins; D013755:Tetradecanoylphorbol Acetate