Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.
Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.
Kazimierczuk
Kacper
K
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
Cova
Lucyna
L
Ndeboko
Benedicte
B
Szczyrk
Urszula
U
Targosz
Aneta
A
Brzozowski
Tomasz
T
Sirko
Agnieszka
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D004372:Ducks / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D014510:Urease / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D000907:Antibodies, Bacterial; C016512:IgY; D007136:Immunoglobulins; D011994:Recombinant Proteins; D014510:Urease