Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma.
A murine monoclonal antibody (mAb), CLD3 (IgG(1),kappa), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix-assisted laser desorption/ionization-time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.
Luk
John M
JM
Department of Surgery, The University of Hong Kong, Pokfulam. jmluk@hku.hk
Su
Yvonne C F
YC
Lam
Stanley C T
SC
Lee
Carol K
CK
Hu
Mei Yu
MY
He
Qing Yu
QY
Lau
George K
GK
Wong
Fred W S
FW
Fan
Sheung-Tat
ST
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000912:Antibodies, Neoplasm / Q000378:metabolism; D034961:Antigens, Nuclear / Q000276:immunology / Q000378:metabolism*; D001666:Binding Sites, Antibody; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D000072200:Ku Autoantigen; D008113:Liver Neoplasms / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen