Assessment of antioxidative activity of lipid- and water-soluble vitamins in human whole blood. Comparative analysis between a biological test and chemical methods.
Assessment of the antioxidant activity of vitamins and other compounds is of interest in the understanding of their in vivo effects. In this study, we have investigated the activity of several lipid and water-soluble vitamins in human whole blood. Measurements were carried out using a biological test that enables the evaluation of both red blood cells and plasma resistance against free radical activity induced by 2,2'-azobis (2-amidinopropane)hydrochloride (AAPH). Antioxidant activity of vitamins has been determined by using the biological test versus chemical methods (chemiluminescence, DMPD radical). We have observed strong anti-oxidant potentials for vitamins B6 and B9 with biological tests, but not with chemical methods. At 10 microM, the vitamin B9 efficiency in inhibiting radical-induced red blood cell hemolysis was almost three times higher than vitamin C efficiency and two times higher than alpha-tocopherol efficiency. Antioxidant activity was not observed for vitamins B1 or B2, nor for retinol. The weak activity of beta-carotene still remains to be investigated particularly in relation to oxygen pressure. Our study demonstrated that the biological test is more useful than the chemical methods employed in this instance, for the evaluation of antioxidant capacity of lipophilic and putatively biologically active compounds.
Lesgards
Jean-François
JF
Institut Méditerranée de Recherche en Nutrition, Centre de St-jérôme, 13397 Marseille cédex 20, France.
Lehucher-Michel
Marie-Pascale
MP
Vidal
Nicolas
N
Prost
Michel
M
Stocker
Pierre
P
D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology; D001681:Biological Assay; D002109:Caffeic Acids / Q000097:blood / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D016877:Oxidants / Q000494:pharmacology; D010655:Phenylenediamines; D012016:Reference Values; D012995:Solubility; D014801:Vitamin A / Q000097:blood / Q000494:pharmacology; D014803:Vitamin B Complex / Q000097:blood / Q000494:pharmacology; D014815:Vitamins / Q000097:blood* / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000097:blood / Q000494:pharmacology; D019207:beta Carotene / Q000097:blood / Q000494:pharmacology
D000578:Amidines; D000975:Antioxidants; D002109:Caffeic Acids; D005456:Fluorescent Dyes; D016877:Oxidants; D010655:Phenylenediamines; D014815:Vitamins; D019207:beta Carotene; D014801:Vitamin A; D014803:Vitamin B Complex; C046728:2,2'-azobis(2-amidinopropane); C004781:dimethyl-4-phenylenediamine; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; C040048:caffeic acid