[The effect of envelope protein mutation on hepatitis B virus assemble].
OBJECTIVE
To investigate the effect of envelope protein mutation on HBV assembly.


METHODS
The envelope protein mutated vectors were constructed by the molecular clone in vitro, and then transfected transiently in the cell HepG2. The expression and secretion of S protein was assay by ELISA. HBV DNA was quantitatively evaluated by PCR. After co-transfection with pHBV-mS1S and adwR9 the DNA was quantitatively evaluated by PCR.


RESULTS
There was no significant difference in expression and secretion of S protein assayed by ELISA in the cytoplasm and supernatant among pHBV-mS1, pHBV-mS, pHBV-mS1S and the wild HBV adwR9 plasmid. The DNA detected by real-time fluorescence quantitative PCR from those the cytoplasm of mutants was higher than that from the wild HBV adwR9 cytoplasm, especially from the cytoplasm of pHBV-mS1S plasmid. However, the DNA detected by real-time fluorescence quantitative PCR from the supernatant of those mutants was lower than that from the wild HBV adwR9 supernatant, especially from pHBV-mS1S. The DNA detected by real-time fluorescence quantitative PCR from the supernatant co-transfected with pHBV-mS1S and adwR9 was lower than that from the supernatant co-transfected with pcDNA3 and adwR9.


CONCLUSIONS
There was no effect of envelope protein mutation on the expression and secretion of S protein. Envelope protein mutation could interfere the assembly of HBV particle and cause reduction of secretion of HBV.
Di
Ya-nan
YN
Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
Hu
Da-rong
DR
Xiong
Jin-hua
JH
Hu
Xue-ling
XL
Li
Juan
J
Fan
Gong-ren
GR
Wu
Yi-pin
YP
D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*; D019065:Virus Assembly
D006514:Hepatitis B Surface Antigens; D014759:Viral Envelope Proteins