title: Inhibition of erythrocyte acetylcholinesterase by n-butanol at high concentrations.
abstract: Erythrocyte acetylcholinesterase (AChE) is bound to the membrane by a complex glycosylphosphatidylinositol anchor, so the effect of alcohol on AChE activity may reflect direct and/or membrane-mediated effects. The indication of a direct interaction between n-butanol and AChE molecules is the activation/inhibition of AChE by occupation of the enzyme's active and/or regulatory sites by alcohol. The activation of AChE can occur only at low concentrations of alcohols, while at high concentrations AChE is inhibited. In this work the mechanism of inhibition of erythrocyte AChE by n-butanol at high concentrations was studied. The values of activity, calculated assuming parabolic competitive inhibition, which implies that one or two molecules of inhibitor bind to the enzyme, fit well to the experimental values. From the values of the inhibition constants it was concluded that at high n-butanol concentrations two alcohol molecules usually interact with AChE.
authors.affiliation: Laboratory of Biophysics, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia. zoran.arsov@ijs.si
authors.forename: Zoran
authors.initials: Z
authors.lastname: Arsov
authors.forename: Matjaz
authors.initials: M
authors.lastname: Zorko
authors.forename: Milan
authors.initials: M
authors.lastname: Schara
mesh_terms: D020001:1-Butanol / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000201:enzymology*
chemical_list: D020001:1-Butanol; D000110:Acetylcholinesterase