Multistress regulation in Escherichia coli: expression of osmB involves two independent promoters responding either to sigmaS or to the RcsCDB His-Asp phosphorelay.
Transcription of the Escherichia coli osmB gene is induced by several stress conditions. osmB is expressed from two promoters, osmBp1 and osmBp2. The downstream promoter, osmBp2, is induced after osmotic shock or upon entry into stationary phase in a sigma(S)-dependent manner. The upstream promoter, osmBp1, is independent of sigma(S) and is activated by RcsB, the response regulator of the His-Asp phosphorelay signal transduction system RcsCDB. RcsB is responsible for the induction of osmBp1 following treatment with chlorpromazine. Activation of osmBp1 by RcsB requires a sequence upstream of its -35 element similar to the RcsB binding site consensus, suggesting a direct regulatory role. osmB appears as another example of a multistress-responsive gene whose transcription involves both a sigma(S)-dependent promoter and a second one independent of sigma(S) but controlled by stress-specific transcription factors.
Boulanger
Alice
A
Laboratoire de Microbiologie et Génétique Moléculaires, UMR5100 Centre National de la Recherche Scientifique-Université Toulouse III, 118 Route de Narbonne, 31062 Toulouse, France.
Francez-Charlot
Anne
A
Conter
Annie
A
Castanié-Cornet
Marie-Pierre
MP
Cam
Kaymeuang
K
Gutierrez
Claude
C
D000222:Adaptation, Physiological; D001224:Aspartic Acid / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D033903:Periplasmic Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C078723:OsmB protein, E coli; D033903:Periplasmic Proteins; C489568:RcsB protein, E coli; D012808:Sigma Factor; D014157:Transcription Factors; C069567:sigma factor KatF protein, Bacteria; C067572:RcsA protein, E coli; D001224:Aspartic Acid; D006639:Histidine