[FTIR microscope study on the change of membrane protein and lipids of erythrocyte damaged by free radicals].
The FTIR Microscope and Computer Aided Analysis were used to study not only the change of secondary structure of erythrocyte membrane protein attacked by the free radicals, but also the effect of oxygen centered free radicals on the membrane lipids in this paper. The results indicate that the content of alpha-helix in the secondary structure of protein clearly changed because of the attack of the free radicals. Damaged by free radicals in 30 minutes, the protein structure could not be restored after 3 hours. The number of the P=O (vp=0), C=O and C=C in the lipids of the membrane was changed. These changes were caused by the peroxidation of the oxygen centered free radicals.
Zhou
Q
Q
Analysis Center, Tsinghua University, 100084 Beijing.
Sun
S
S
Zhang
S
S
Hu
X
X
Huang
Y
Y
Zhao
H
H
Liu
D
D
D000328:Adult; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008853:Microscopy; D017433:Protein Structure, Secondary / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared
D005609:Free Radicals; D008563:Membrane Lipids; D008565:Membrane Proteins