title: Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4-sulfophenyl isothiocyanate.
abstract: Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4-sulfophenyl isothiocyanate derivatives, generated by matrix-assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed.
authors.affiliation: National Creative Research Initiative Center for Control of Reaction Dynamics and School of Chemistry, Seoul National University, Korea.
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Oh
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Moon
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Hyung
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001557:Benzenesulfonates / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013861:Thiocyanates / Q000737:chemistry*
chemical_list: D001557:Benzenesulfonates; D017879:Isothiocyanates; D010455:Peptides; D013861:Thiocyanates; C005441:phenylisothiocyanate; C015308:4-isothiocyanatobenzenesulfonate