title: Evidence for disruption of Na(+)-K(+)-ATPase and hsp70 during vibriosis of sea bream, Sparus (=Rhabdosargus) sarba Forsskål.
abstract: Osmoregulation via the sodium pump (Na(+)-K(+)-ATPase) and cytoprotection via expression of different families of heat shock protein (hsp) were studied at transcriptional/translational levels during progressive vibriosis in silver sea bream, Sparus (=Rhabdosargus) sarba Measurements of Na(+)-K(+)-ATPase activity showed that an early and drastic decline occurred in the kidney of infected fish. This reduction in Na(+)-K(+)-ATPase activity was not caused by transcriptional downregulation of genes coding for either the Na(+)-K(+)-ATPase alpha or beta subunits. Using specific antibodies, data from immunoassays showed that the decreased sodium pump activity was caused by the specific loss of the translated glycosylated Na(+)-K(+)-ATPase beta subunit. Data from immunoassays of different hsp families demonstrated that the expression of hsp90 and hsp60 remained unchanged throughout vibriosis whereas expression of the hsp70 family decreased in kidney and liver tissues. As hsp70 is a multigene family, the expression of the constitutive (hsc70) and inducible (hsp70) members of the hsp70 family were studied and it was found that hsp70 and hsc70 expression decreased from an early stage of infection in the kidney and the liver respectively. Reverse transcriptase-polymerase chain reaction analysis of the hsp70 transcription-inducing factor, hsf1, demonstrated that loss of cytoprotective function during vibriosis was mediated by a downregulation of hsf1 transcription.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
authors.forename: E E
authors.initials: EE
authors.lastname: Deane
authors.forename: N Y S
authors.initials: NY
authors.lastname: Woo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000378:metabolism* / Q000382:microbiology*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D007118:Immunoassay; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010473:Perciformes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014157:Transcription Factors; D014735:Vibrio Infections / Q000378:metabolism / Q000662:veterinary*; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors; D000254:Sodium-Potassium-Exchanging ATPase