title: Thiolate coordination to Fe(II)-porphyrin NO centers.
abstract: The interaction of the Fe(II)-porphyrin NO model complex [Fe(TPP)(NO)] (1, TPP=tetraphenylporphyrin) with thiophenolate ligands and tetrahydrothiophene is explored both computationally and experimentally. Complex 1 is reacted with substituted thiophenolates and the obtained six-coordinate adducts of type [Fe(TPP)(SR)(NO)](-) are investigated in solution using electron paramagnetic resonance (EPR) spectroscopy. From the obtained g values and (14)N hyperfine pattern of the NO ligand it is concluded that the interaction of the thiophenolates with the Fe(II) center is weak in comparison to the corresponding 1-methylimidazole adduct. The strength of the Fe-S bond is increased when alkylthiolates are used as evidenced by comparison with the published EPR spectra of ferrous NO adducts in cytochromes P450 and P450nor, which have an axial cysteinate ligand. These results are further evaluated by density functional (DFT) calculations. The six-coordinate model complex [Fe(P)(SMe)(NO)](-) (1-SMe; P=porphine ligand used for the calculations) has an interesting electronic structure where NO acts as a medium strong sigma donor and pi acceptor ligand. Compared to the N-donor adducts with 1-methylimidazole (1-MeIm), etc., donation from the pi(h)( *) orbital of NO to Fe(II) is reduced due to the stronger trans effect of the alkylthiolate ligand. This is reflected by the predicted longer Fe-NO bond length and smaller Fe-NO force constant for 1-SMe compared to the 1-MeIm adduct. Therefore, the Fe(II)-porphyrin NO adducts with trans alkylthiolate coordination have to be described as Fe(II)-NO(radical) systems. The N-O stretching frequency of these complexes is predicted below 1600cm(-1) in agreement with the available experimental data. In addition, 1-SMe has a unique spin density distribution where Fe has a negative spin density of -0.26 from the calculations. The implications of this unusual electronic structure for the reactivity of the Fe(II)-NO alkylthiolate adducts as they occur in cytochrome P450nor are discussed.
authors.affiliation: Institut für Anorganische Chemie, Christian-Albrechts-Universität Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany.
authors.forename: V K K
authors.initials: VK
authors.lastname: Praneeth
authors.forename: Erhard
authors.initials: E
authors.lastname: Haupt
authors.forename: Nicolai
authors.initials: N
authors.lastname: Lehnert
mesh_terms: D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D008024:Ligands; D008665:Metalloporphyrins / Q000737:chemistry*; D008954:Models, Biological; D008958:Models, Molecular; D009569:Nitric Oxide / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D007093:Imidazoles; D008024:Ligands; D008665:Metalloporphyrins; D010636:Phenols; D011166:Porphyrins; D013438:Sulfhydryl Compounds; D013876:Thiophenes; D009569:Nitric Oxide; C107018:porphine; C121030:tetrahydrothiophene; C042983:thiophenol; D003577:Cytochrome P-450 Enzyme System; D003545:Cysteine; C018100:1-methylimidazole