Study of enrofloxacin depletion in the eggs of laying hens using diphasic dialysis extraction/purification and determinative HPLC-MS analysis.
A study of the depletion of enrofloxacin residues in eggs was carried out using a diphasic dialysis procedure for the extraction of fluoroquinolone residues from the matrix. Enrofloxacin was administered to laying hens through the intramuscular route (15 mg/day) and orally (12 mg/day). After daily collection, the egg albumen and the egg yolk were separated, and the residue levels were determined using an HPLC-MS (API-ESI) method. The enrofloxacin and ciprofloxacin peaks gradually increased until the fifth day, because the drug was employed for 5 days. However, differences were observed in the depletion curves of enrofloxacin and its metabolite when both parts of the egg and the mode of administration were considered.
Lolo
M
M
Laboratorio de Higiene, Inspección y Control de Alimentos, Area de Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, E-27002 Lugo, Spain.
Pedreira
S
S
Fente
C
C
Vázquez
B I
BI
Franco
C M
CM
Cepeda
A
A
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis; D003956:Dialysis; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000032:analysis*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D015363:Quinolones / Q000008:administration & dosage / Q000032:analysis*
D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin