Molecular diversity of Proteus mirabilis isolates producing extended-spectrum beta-lactamases in a French university hospital.
Between February 1997 and December 2002, 3340 hospitalised patients yielded samples positive for Proteus mirabilis, of whom 45 (1.3%) were colonised/infected by P. mirabilis producing extended-spectrum beta-lactamases (ESBLs). The gross incidence of patients colonised/infected by ESBL-producing P. mirabilis was 1.61/10(5) days of hospitalisation, with 20% of isolates being collected from patients in urology wards, most frequently (53.3%) from urine samples. Seventeen (37.7%) of the 43 isolates were obtained from samples collected within 48 h of hospitalisation, indicating that they were community-acquired. Isoelectric focusing assays and sequencing identified the TEM-24, TEM-92 and TEM-52 ESBLs. Pulsed-field gel electrophoresis revealed eight pulsotypes (I-VIII), with the two most common pulsotypes, IV and VI, comprising ten (23.3%) and 12 (26.6%) isolates, respectively. These pulsotypes were considered to represent epidemic strains and spread in various wards of the hospital.
Biendo
M
M
Laboratoire de Bactériologie et Hygiène, CHU Nord, France. Biendo.Maurice@chu-amiens.fr
Thomas
D
D
Laurans
G
G
Hamdad-Daoudi
F
F
Canarelli
B
B
Rousseau
F
F
Castelain
S
S
Eb
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017714:Community-Acquired Infections / Q000453:epidemiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D006785:Hospitals, University; D006801:Humans; D008297:Male; D015995:Prevalence; D011512:Proteus Infections / Q000453:epidemiology* / Q000382:microbiology / Q000652:urine; D011513:Proteus mirabilis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; C445691:TEM-52 beta-lactamase; D001618:beta-Lactamases