title: Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain.
abstract: The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.
authors.affiliation: Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China.
authors.forename: Chuan-hai
authors.initials: CH
authors.lastname: Zhang
authors.forename: Jia-hai
authors.initials: JH
authors.lastname: Lu
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Huan-ying
authors.initials: HY
authors.lastname: Zheng
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong
authors.forename: Mei-ying
authors.initials: MY
authors.lastname: Zhang
authors.forename: Xin-jian
authors.initials: XJ
authors.lastname: Liu
authors.forename: Jiu-xiang
authors.initials: JX
authors.lastname: Li
authors.forename: Zhuo-yue
authors.initials: ZY
authors.lastname: Wan
authors.forename: Xin-ge
authors.initials: XG
authors.lastname: Yan
authors.forename: Shu-Yuan
authors.initials: SY
authors.lastname: Qi
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Cui
authors.forename: Biliang
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D015164:Vaccines, Inactivated; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015164:Vaccines, Inactivated; D014765:Viral Vaccines