Tannerella forsythia-induced alveolar bone loss in mice involves leucine-rich-repeat BspA protein.
Tannerella forsythia (formerly Bacteroides forsythus) is one of the periodontal pathogens recently implicated in the development of periodontal disease. The cell-surface-associated, as well as the secreted, leucine-rich-repeat protein (BspA) of this bacterium have been suggested to play roles in bacterial adherence, and also in inflammation, by triggering release of pro-inflammatory cytokines from monocytes and chemokines from osteoblasts, leading to inflammation and bone resorption. In this study, we sought to determine the pathogenic potential of T. forsythia and the in vivo role of the BspA protein in pathogenesis in the mouse model of infection-induced alveolar bone loss. The results showed alveolar bone loss in mice infected with the T. forsythia wild-type strain, whereas the BspA mutant was impaired. In conclusion, evidence is presented in support of T. forsythia as an important organism involved in inducing alveolar bone loss, and the BspA protein is an important virulence factor of this bacterium.
Sharma
A
A
Department of Oral Biology, School of Dental Medicine, State University of New York, 3435 Main Street, Buffalo, NY 14214, USA. sharmaa@buffalo.edu
Inagaki
S
S
Honma
K
K
Sfintescu
C
C
Baker
P J
PJ
Evans
R T
RT
D016301:Alveolar Bone Loss / Q000276:immunology / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000502:physiology*; D001426:Bacterial Proteins / Q000276:immunology / Q000502:physiology*; D001439:Bacteroides / Q000276:immunology / Q000472:pathogenicity*; D001442:Bacteroides Infections / Q000276:immunology / Q000382:microbiology*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D000090263:Leucine-Rich Repeat Proteins; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011506:Proteins / Q000276:immunology / Q000502:physiology*; D020449:Repetitive Sequences, Amino Acid / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D037521:Virulence Factors / Q000502:physiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C116104:BspA protein, Bacteroides; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D037521:Virulence Factors