[Construction and characteristics analysis of somatic cell lines from common carp Cyprinus carpio].
Epithelia of rostral side and fins from common carp were taken to culture in vitro. As a result, cells of tail fins were successfully passed from generation to generation, and constructed cell lines. During the culture procedure, the passage cells were observed and their biological characteristics were analyzed, which consist of morphologic analysis, drawing of growth curve, detection of producing rate for clones, insight of adaptation to temperatures, calculation of chromosomes and investigation for thaw of frozen cells. According to the observation results, we found that (1) there is an increase trend of fibroblast. epithelia of rostral side and tail fin from common carp are the best materials for culturing cells; (2) the optimum culturing temperature is 27 degrees C; (3) there isn't any change in cell chromosomes, which still are normal diploid. However, with, the generations increasing, the mode of chromosome has an inclination of decrease in normal ranges. We inferred the donor cells from 4-10 generations are the best choice for nuclear transplanted experiments in order to guarantee their genetic stabilities and survival rates after transplanting the nuclei. And, the cells will grow extremely well when cultured for 48-72h at 27 degrees C. We suggest the temperature should be descended little by little involving in preserving cells with DMSO. So far, the cells were passed 30th generations and part of them was preserved into liquid nitrogen.
Meng
Fan Hua
FH
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070.
Yin
Hong Bin
HB
Sun
Zhong Wu
ZW
Chen
Wei Duo
WD
D000818:Animals; D002347:Carps / Q000254:growth & development* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism