Barley Rom1 reveals a potential link between race-specific and nonhost resistance responses to powdery mildew fungi.
The Rar1 gene, identified in the context of race-specific powdery mildew resistance mediated by the Hordeum vulgare (barley) resistance (R) gene Mla12, is required for the function of many R-mediated defense responses in mono- and dicotyledonous plant species. Mla resistance is associated with an oxidative burst and a subsequent cell death reaction of attacked cells. Rar1 mutants are impaired in these responses and, to identify genetic elements which negatively regulate the Mla12-triggered response, we have screened mutagenized Mla12 rar1 mutant populations for restoration of the resistance response. Here we describe the restoration of Mla12-specified resistance (rom1) mutant that restores features of disease resistance to a Blumeria graminis f. sp. hordei isolate expressing the avirulence gene AvrMla12 and retains susceptibility to an isolate lacking AvrMla12. Histochemical analyses show that, in rom1 mutant plants, a whole-cell oxidative burst and cell death response in attacked epidermal cells is restored in the incompatible interaction. Defense responses against tested inappropriate powdery mildews, B. graminis f. sp. tritici and Golovinomyces orontii, were diminished in rar1 mutant plants and enhanced in rom1 mutant plants relative to the wild type. These findings indicate antagonistic activities of Rar1 and Rom1 and reveal their contribution to nonhost and race-specific resistance responses.
Freialdenhoven
Andreas
A
Max-Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany. freiald@freenet.de
Orme
James
J
Lahaye
Thomas
T
Schulze-Lefert
Paul
P
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005658:Fungi / Q000472:pathogenicity*; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins; D016296:Mutagenesis; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D016897:Respiratory Burst
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C433105:MLA1 protein, Hordeum vulgare; D010940:Plant Proteins; C499582:Rar1 protein, Hordeum vulgare; D006861:Hydrogen Peroxide