Osteolytic osteomyelitis associated with visceral leishmaniasis in a dog.
A dog was examined with a history of weight loss and lameness of the left hind limb. A painful response to examination of the left hip joint, and lymphadenopathy were noted. Amastigote forms of Leishmania sp. were observed by cytology in samples from the popliteus lymph node, and anti-Leishmania sp. antibodies at a titer of 1:640 were detected in serum by indirect immunofluorescence. Radiological changes included osteolysis and a periosteal proliferative reaction in the left femoral greater trochanter. These changes were histologically characterized as an osteolytic granulomatous osteomyelitis associated with amastigotes within macrophages. Non-decalcified fragments of the periosteum were processed for immunohistochemistry, observed with prominent immunolabelling of amastigotes of Leishmania sp. within macrophages. The diagnosis was further confirmed by positive PCR for Leishmania sp., belonging to the Leishmania donovani complex.
de Souza
Alda Izabel
AI
Curso de Medicina Veterinária, Centro de Ciências Biológicas, Agrárias e da Saúde (CCBAS), Universidade para o Desenvolvimento do Estado e da Região do Pantanal (UNIDERP), Rua Alexandre Herculano 1400, 79037-280 Campo Grande, MS, Brazil. alda@nin.ufms.br
Juliano
Raquel Soares
RS
Gomes
Tatiana Scanoni
TS
de Araujo Diniz
Soraia
S
Borges
Alan Maia
AM
Tafuri
Wagner Luiz
WL
Santos
Renato Lima
RL
D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D004285:Dogs; D017809:Fatal Outcome; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006614:Hindlimb / Q000000981:diagnostic imaging / Q000469:parasitology / Q000473:pathology; D007893:Leishmania donovani / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D008264:Macrophages / Q000469:parasitology; D008297:Male; D010019:Osteomyelitis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D011859:Radiography