Enhanced swarming of bacteria on agar plates containing the surfactant Tween 80.
A widely used method for quantifying swarming motility is the swarm plate assay. A significant increase in the motility halo size formed by Escherichia coli or Azospirillum brasilense was measured on Tween 80-containing agar relative to untreated agar. This improvement could benefit the identification of mutants in swarming motility.
Niu
Chen
C
Department of Biology, Georgia State University, Atlanta, GA 30303, USA.
Graves
Jane D
JD
Mokuolu
Felicia O
FO
Gilbert
Sarah E
SE
Gilbert
Eric S
ES
D000362:Agar; D015806:Azospirillum brasilense / Q000502:physiology*; D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D011136:Polysorbates; D013501:Surface-Active Agents
D011136:Polysorbates; D013501:Surface-Active Agents; D000362:Agar