Psychophysiological stress-regulated gene expression in mice.
Eight genes showed significant changes in expression in mice under psychophysiological stress provided by cage-restraint and water-immersion. The transcription level of most of these genes was affected in all the tissues analyzed, and some of them were responsive genes in several different stress systems. Peculiarly, the expression level of one gene, cdc2-like kinase 1 (CLK1), was reduced only in the brain, while the balance of partially- and alternatively-spliced CLK1 mRNA species changed in all the tissues including the brain. These results suggest that some stress-response mechanisms, including transcriptional and post-transcriptional events, are coordinated in the whole body in mice under psychophysiological stress.
Murata
Shigenori
S
DNA Chip Research Inc., 1-1-43 Suehiro, Tsurumi, Yokohama 230-0045, Japan. murata@dna-chip.co.jp
Yoshiara
Taeko
T
Lim
Chun Ren
CR
Sugino
Maiko
M
Kogure
Mitori
M
Ohnuki
Tatsuya
T
Komurasaki
Toshi
T
Matsubara
Kenichi
K
D000818:Animals; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D017874:Immediate-Early Proteins; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013315:Stress, Psychological / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D000099184:Serum-Glucocorticoid Regulated Kinases
D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases; C066780:Clk dual-specificity kinases