A DNA pooling strategy for family-based association studies.
Genome-wide association scans for disease susceptibility genes of complex diseases require genotyping on a massive scale. A DNA pooling strategy for family-based association studies is described, which is robust to population stratification biases and to errors in pooling. It can achieve a statistical efficiency of 0.95 with approximately 1 of 8 or fewer genotyping efforts, and an efficiency of 0.90 with approximately 1 of 16 or fewer efforts compared with individual genotyping. The pooling method described in this article provides a tradeoff between genotyping efforts and subject recruitment efforts.
Lee
Wen-Chung
WC
Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan. wenchung@ha.mc.ntu.edu.tw
D000483:Alleles; D004247:DNA / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005788:Gene Pool; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D011336:Probability
D004247:DNA