title: [Quantitative analysis of the nucleosides in Cordyceps sinensis with capillary zone electrophoresis].
abstract: OBJECTIVE
  To establish a quantitative analysis method for analyzing the nucleosides in Cordyceps sinensis with capillary electrophoresis, and compare the difference between natural and the cultured C. mycelia.
  
  
  METHODS
  Capillary zone electrophoresis method was employed to quantitate the adenosine, uridine, guanosine and inosine in C. sinensis, with 0.25 mg x L(-1) boric acid-sodium hydroxide buffer, pH 9.5. The working voltage was 20 kV, the temperature was 25 degrees C, and the detection wavelength was 260 nm.
  
  
  RESULTS
  With the capillary zone electrophoresis method, the average recovery of the above 4 nucleosides was 98.9%, 95.1%, 97.8% and 98.8% respectively, with the RSD 0.4%, 1.7%, 1.3% and 5.0%. There was no adenosine in natural C. sinensis and no inosine in the cultured C. mycelia detected.
  
  
  CONCLUSIONS
  This method can be used to determine the adenosine, uridine, guanosine and inosine in C. sinensis. The nucleosides in C. sinensis produced from Qinghai province and cultured C. mycelia are obviously different.
authors.affiliation: Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310027, China.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Hou
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Luan
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Cheng
mesh_terms: D000241:Adenosine / Q000032:analysis*; D000818:Animals; D032661:Cordyceps / Q000737:chemistry* / Q000145:classification; D046508:Culture Techniques; D019075:Electrophoresis, Capillary / Q000379:methods; D006151:Guanosine / Q000032:analysis; D007288:Inosine / Q000032:analysis*; D007915:Lepidoptera / Q000737:chemistry; D014529:Uridine / Q000032:analysis
chemical_list: D006151:Guanosine; D007288:Inosine; D000241:Adenosine; D014529:Uridine