title: Multiplex PCR for typing strains of Toxoplasma gondii.
abstract: A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.
authors.affiliation: Laboratoire de Parasitology-Mycologie, Faculté de Médecine EA 3174, Limoges, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
authors.forename: Aurélien
authors.initials: A
authors.lastname: Dumètre
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Dardé
mesh_terms: D000818:Animals; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics