Morphological studies in modern teratological investigations.
Despite the variety of modern molecular techniques available, examination of foetal anatomy is still a fundamental part of teratological studies in evaluating the developmental toxicity of xenobiotics or other non-chemical factors. The article presents contemporary methods of embryotoxicity and foetotoxicity assessment. A single alizarin red S and double alcian blue followed by alizarin red S staining, as well as various methods of soft tissue examination are discussed.
Burdan
Franciszek
F
Experimental Teratology Unit, Department of Human Anatomy, Medical University of Lublin, 20-074 Lublin, Poland. fb3@wp.pl
Szumiło
Justyna
J
Dudka
Jarosław
J
Klepacz
Robert
R
Błaszczak
Magdalena
M
Solecki
Michał
M
Korobowicz
Agnieszka
A
Chałas
Anna
A
Klepacki
Janusz
J
Palczak
Michał
M
Zuchnik-Wrona
Agata
A
Hadała-Kiś
Anna
A
Urbanowicz
Zygmunt
Z
Wójtowicz
Zbigniew
Z
D000423:Alcian Blue / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D005260:Female; D005333:Fetus / Q000033:anatomy & histology* / Q000187:drug effects; D006801:Humans; D018811:Maternal Exposure; D051381:Rats; D018600:Teratology / Q000379:methods; D015262:Xenobiotics / Q000494:pharmacology
D000880:Anthraquinones; D004396:Coloring Agents; D015262:Xenobiotics; C004468:Alizarin Red S; D000423:Alcian Blue