title: Preclinical experiment of auxiliary partial orthotopic liver transplantation as a curative treatment for hemophilia.
abstract: The cause of hemophilia is deficiency of coagulation factor VIII production in the liver, which can be cured by liver transplantation. Because the hepatic function of hemophilia patients is quite normal except for production of factor VIII, auxiliary partial orthotopic liver transplantation (APOLT) is beneficial in that patient survival is secured by preserving native liver even in the event of graft loss. However, it is not known whether the graft of APOLT would be enough to cure hemophilia. We evaluated the efficacy and feasibility of APOLT for hemophilia in a canine hemophilia A model that we established. Partial left liver graft was taken from the normal donor (blood factor VIII activity > 60%). The graft was transplanted to the hemophilia beagle dog (blood factor VIII activity < 5%) after resection of the left lobe preserving native right lobe. Changes in time of blood factor VIII activity and liver function parameters were observed after APOLT. APOLT and perioperative hemostatic management were successfully performed. The blood factor VIII activity increased to 30% after APOLT, and was sustained at least 6 weeks throughout the observation period without symptoms of bleeding. The result demonstrated sustained production of factor VIII in the hemophilia recipient after APOLT. Transplantation of approximately one third of whole liver resulted in cure of hemophilia. In conclusion, it is suggested that APOLT would be feasible as a curative treatment of hemophilia A to improve quality of life of the patients.
authors.affiliation: Department of Surgery, Nara Medical University, Kashihara, Nara, Japan. saihoko@naramed-u.ac.jp
authors.forename: Saiho
authors.initials: S
authors.lastname: Ko
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tanaka
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Kanehiro
authors.forename: Hideki
authors.initials: H
authors.lastname: Kanokogi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Ori
authors.forename: Midori
authors.initials: M
authors.lastname: Shima
authors.forename: Akira
authors.initials: A
authors.lastname: Yoshioka
authors.forename: Alan
authors.initials: A
authors.lastname: Giles
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005169:Factor VIII / Q000378:metabolism; D006085:Graft Survival; D006467:Hemophilia A / Q000097:blood / Q000601:surgery*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D014184:Transplantation, Homologous
chemical_list: D005169:Factor VIII