The relationship between bone metastasis from human breast cancer and integrin alpha(v)beta3 expression.
BACKGROUND
Osteoclast activation plays an essential role in the development of bone metastasis (BM). Integrin alpha(v)beta3 mediates the attachment of osteoclasts to bone matrix, and it is overexpressed in bone-residing breast cancer cells. We studied BM from breast cancer in relation to its Integrin alpha(v)beta3 expression.


METHODS
Integrin alpha(v)beta3 expression in 4 human breast cancer cell lines (MDA-MB-231, MDA-MB-435, MKL-4 and T-47D) was determined by immunohistochemistry, flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
Integrin alpha(v)beta3 was expressed in MDA-MB-231 and MDA-MB-435, but not in MKL-4 or T-47D, using both immunohistochemistry and flow cytometry. By RT-PCR, positive bands for both alpha(v) and beta3 subunits were identified in MDA-MB-231 and 435, while only the alpha(v) subunit mRNA was present in MKL-4 and T-47D. BM was common in vivo following inoculation with MDA-MB-231 or 435, but not with MKL-4.


CONCLUSIONS
Integrin alpha(v)beta3 expression appears to play a key role in the development of BM from breast cancer.
Takayama
Shin
S
Department of Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. stakayam@sc.itc.keio.ac.jp
Ishii
Seiichiro
S
Ikeda
Tadashi
T
Masamura
Shigeru
S
Doi
Masakazu
M
Kitajima
Masaki
M
D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000556:secondary*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D039302:Integrin alphaVbeta3 / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous
D039302:Integrin alphaVbeta3