title: Stable expression of a defense-related gene in wheat epidermis under transcriptional control of a novel promoter confers pathogen resistance.
abstract: Tissue-specific or regulated expression of transgenes is desirable in order to prevent pleiotropic side effects of putatively harmful transgene products as well as loss of energy resources due to unnecessary accumulation of transgene products. Epidermis-specific expression would be useful for many defense-related genes directed against attack by fungal pathogens that enter the plant body by direct penetration through the epidermis. In an approach to enhance resistance of wheat to the powdery mildew fungus Blumeria graminis f.sp. tritici, a novel epidermis-specific promoter was developed and used for expression of two defense-related genes. A 2.3 kb fragment of the wheat GstA1 promoter in combination with an intron-containing part of the wheat WIR1a gene was found to drive strong and constitutive transient expression in wheat epidermis. This promoter-intron combination was used for overexpression of oxalate oxidase 9f-2.8 and TaPERO peroxidase, two defense-related wheat genes expressed in inner leaf tissues. Expression studies of several transgenic lines by in situ oxalate-oxidase staining, RNA and protein blot analyses, as well as real-time PCR, demonstrated strong and constitutive transgene expression in the shoot epidermis. Transient as well as stable over-expression of the TaPERO peroxidase gene in wheat epidermis under the control of the GstA1i promoter resulted in enhanced resistance against Blumeria graminis f.sp. tritici, whereas oxalate-oxidase overexpression had no effect in either system. The data suggest that the wheat GstA1 promoter in combination with the WIR1a intron is useful for transgenic approaches to fungal disease resistance in cereals.
authors.affiliation: Agronomy Department, PMCB, Laboratory of Molecular Plant Physiology, University of Florida-IFAS, 2191 McCarty Hall, PO Box 110300, Gainesville, FL 32611-0300, USA.
authors.forename: Fredy
authors.initials: F
authors.lastname: Altpeter
authors.forename: Alok
authors.initials: A
authors.lastname: Varshney
authors.forename: Olaf
authors.initials: O
authors.lastname: Abderhalden
authors.forename: Dimitar
authors.initials: D
authors.lastname: Douchkov
authors.forename: Christof
authors.initials: C
authors.lastname: Sautter
authors.forename: Jochen
authors.initials: J
authors.lastname: Kumlehn
authors.forename: Robert
authors.initials: R
authors.lastname: Dudler
authors.forename: Patrick
authors.initials: P
authors.lastname: Schweizer
mesh_terms: D001203:Ascomycota / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D019441:Plant Epidermis / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; D009195:Peroxidase; C020598:oxalate oxidase