Viral RNA is required for the association of APOBEC3G with human immunodeficiency virus type 1 nucleoprotein complexes.
APOBEC3G (APO3G) is a host cytidine deaminase that is incorporated into human immunodeficiency virus type 1 (HIV-1) particles. We report here that viral RNA promotes stable association of APO3G with HIV-1 nucleoprotein complexes (NPC). A target sequence located within the 5'-untranslated region of the HIV-1 RNA was identified to be necessary and sufficient for efficient APO3G packaging. Fine mapping revealed a sequence normally involved in viral genomic RNA dimerization and Gag binding to be important for APO3G packaging and association with viral NPC. Our data suggest that packaging of APO3G into HIV-1 NPC is enhanced by viral RNA.
Khan
Mohammad A
MA
NIH, NIAID, 4/312, 4 Center Drive, MSC 0460, Bethesda, MD 20892-0460, USA.
Kao
Sandra
S
Miyagi
Eri
E
Takeuchi
Hiroaki
H
Goila-Gaur
Ritu
R
Opi
Sandrine
S
Gipson
Clay L
CL
Parslow
Tristram G
TG
Ly
Hinh
H
Strebel
Klaus
K
D020121:5' Untranslated Regions / Q000378:metabolism; D000071480:APOBEC-3G Deaminase; D003564:Cytidine Deaminase; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009698:Nucleoproteins / Q000378:metabolism*; D009700:Nucleoside Deaminases; D011506:Proteins / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D012097:Repressor Proteins; D014779:Virus Replication
D020121:5' Untranslated Regions; D009698:Nucleoproteins; D011506:Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; D009700:Nucleoside Deaminases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase