title: Study of Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile for odorant biosensors.
abstract: To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we have investigated mixed Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile. Under optimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration of 4 mg L(-1), target pressure 35 mN m(-1)), the mixed monolayer at the air/water interface is very stable and has been efficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers (SAMs) with 1-octadecanethiol (ODT). Atomic force microscopy and electrochemical impedance spectroscopy were used to characterize mixed Langmuir-Blodgett (LB) films before and after contact with a specific odorant molecule, isoamyl acetate. AFM phase images show a higher contrast after contact with the odorant molecule due to the new structure of the OBP-1F/ODA LB film. Non-Faradaic electrochemical spectroscopy (EIS) is used to quantify the effect of the odorant based on the electrical properties of the OBP-1F/ODA LB film, as its resistance strongly decreases from 1.18 MOmega (before contact) to 25 kOmega (after contact).
authors.affiliation: Centre de Génie Electrique de Lyon (CEGELY), Ecole Centrale de Lyon, 69134 ECULLY Cedex, France. yanxiahou24@yahoo.com
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Hou
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: Claude
authors.initials: C
authors.lastname: Martelet
authors.forename: Chaker
authors.initials: C
authors.lastname: Tlili
authors.forename: Aidong
authors.initials: A
authors.lastname: Zhang
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Pernollet
authors.forename: Loïc
authors.initials: L
authors.lastname: Briand
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gomila
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Errachid
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
authors.forename: Ludovic
authors.initials: L
authors.lastname: Salvagnac
authors.forename: Benoit
authors.initials: B
authors.lastname: Torbiéro
authors.forename: Pierre
authors.initials: P
authors.lastname: Temple-Boyer
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry; D000439:Pentanols / Q000737:chemistry; D018035:Receptors, Odorant / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000439:Pentanols; D018035:Receptors, Odorant; D013438:Sulfhydryl Compounds; C045648:odorant-binding protein; C055360:n-octadecyl mercaptan; D006046:Gold; C020377:isoamyl acetate