[Studies on the genetic variation of two mitochondrial DNA molecules of Schistosoma japonicum].
OBJECTIVE
To study the variation of Schistosoma japonicum through two mitochondrial DNA molecules.


METHODS
Genomic DNA was isolated with kit, and the mitochondrial NADH dehydrogenase 1 (ND1) and cytochrome c oxidase I (COI) gene fragments were amplified by polymerase chain reaction (PCR) and sequenced. The gene trees were constructed and the acquired data were analyzed with the help of bioinformatics.


RESULTS
The gene trees showed that the Taiwan isolate and the mainland isolates can be divided in two groups: a group from the hilly region (Yunnan and Sichuan), another group from the lake region (Hunan, Jiangxi and Anhui); isolates from Hubei are at a different position on the gene trees.


CONCLUSIONS
There are variations among the geographic isolates of Schistosoma japonicum in China, nevertheless, they have close kinship.
Guo
Kai-wen
KW
Department of Parasitology, Tongji Medical College, Huazhong University of Technology and Science, Wuhan 430030, China.
Niu
An-ou
AO
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D042967:Electron Transport Complex I / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics*
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I