[Study on the interaction of copper-zinc superoxide dismutase with cobalt (II)-histidine by spectral analysis--II. Effect of amount of external added Co(His)n, phosphate].
The effect of changing the amount of external added Co(His)n and phosphate on the interaction of Cu2Zn2SOD with organic metal compound (Cobalt (II)-Histidine) have been studied by means of ICP, VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added Co(His)n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co (II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt (II)-Histidine) and of the presence of phosphate and got some results under the effect of corresponding factors.
Zheng
X
X
Danling Institute of Chemical Physics, Academia Sinica, 116023 Dalian.
Hu
J
J
Cheng
G
G
Xu
Y
Y
Zhao
Y
Y
Liu
M
M
D003035:Cobalt / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011485:Protein Binding; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000737:chemistry*
D008667:Metalloproteins; D010710:Phosphates; D003035:Cobalt; D006639:Histidine; D013482:Superoxide Dismutase