title: Class-specific binding of two aminoacyl-tRNA synthetases to annexin, a Ca2+- and phospholipid-binding protein.
abstract: Annexins are a family of Ca2+/phospholipid-binding proteins that have diverse functions. To understand the function of annexin in Physarum polycephalum, we searched for its binding proteins. Here we demonstrate the presence of two novel annexin-binding proteins. The homology search of partial amino acid sequences of these two proteins identified them as aminoacyl-tRNA synthetases (ARSs). Furthermore, antibody against aminoacyl-tRNA synthetases cross-reacted with one of two proteins. Our results imply the interaction between intracellular membrane dynamics and protein translation system, and may give a clue to understand the mechanism of some myositis diseases, which have been known to produce autoantibodies against ARSs.
authors.affiliation: Graduate School of Science, Biological Science, Osaka University, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Akio
authors.initials: A
authors.lastname: Nakamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kohama
authors.forename: Michito
authors.initials: M
authors.lastname: Hirakata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogihara
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D000818:Animals; D017302:Annexins / Q000378:metabolism*; D026481:Blotting, Far-Western; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D016839:Physarum polycephalum / Q000201:enzymology / Q000254:growth & development; D011233:Precipitin Tests; D011485:Protein Binding; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: D017302:Annexins; D010743:Phospholipids; D000604:Amino Acyl-tRNA Synthetases; D002118:Calcium