Differential interplay between protein kinase C and Rho-kinase in the endothelin-1-induced and pressure-induced contractions of rat posterior cerebral artery.
To clarify the involvement of protein kinase C and Rho-kinase in the contractile activation of cerebral artery in response to endothelin-1 and pressurization, rat posterior cerebral artery (outer diameter, 100-200 microm) was mounted in arteriograph, and the changes in cytosolic Ca2+ and vessel diameter were measured by video-microscopy in connection with an Argus 50 system. Endothelin-1 (10 nM) induced a tonic contraction with a slight increase in cytosolic Ca2+, which was mostly dependent on protein kinase C (chelerythrine sensitive). Intraluminal pressurization (60 mmHg) also produced contraction with a low cytosolic Ca2+, which was myogenic in nature and dependent on both protein kinase C and Rho-kinase (Y-27632 sensitive). The results suggest differential interplay between protein kinase C and Rho-kinase in the endothelin-1-induced and pressure-induced tonic phase of contractions in the rat posterior cerebral artery.
Okamoto
Yoshiaki
Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka, Shizuoka City, Japan.
Kashihara
Toshihide
T
Nonogaki
Atsushi
A
Obara
Kazuo
K
Nakayama
Koichi
K
D000577:Amides / Q000494:pharmacology; D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology; D001794:Blood Pressure; D020013:Calcium Signaling / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism*; D066298:In Vitro Techniques; D040542:Mechanotransduction, Cellular / Q000187:drug effects; D020769:Posterior Cerebral Artery / Q000187:drug effects / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D014661:Vasoconstriction / Q000187:drug effects; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000577:Amides; D053119:Benzophenanthridines; D019332:Endothelin-1; D047428:Protein Kinase Inhibitors; D011725:Pyridines; C108830:Y 27632; C016299:chelerythrine; D054460:rho-Associated Kinases; D011493:Protein Kinase C