title: Immunohistochemical characterization of the cellular infiltrate in Jorge Lobo's disease.
abstract: Few studies have been conducted to evaluate the cellular composition of the granulomatous lesions induced by Lacazia loboi. Thus, the objective of the present study was to characterize the mononuclear cell population present in cutaneous lesions obtained from 15 patients with Jorge Lobo's disease. Histological sections were stained with hematoxylin-eosin and methenamine silver and the following mononuclear cells were identified by immunohistochemistry: T lymphocytes (CD3+), helper T lymphocytes (CD4+), cytotoxic T lymphocytes (CD8+), B lymphocytes (CD20+), plasma cells (CD79+), natural killer cells (CD57+) and histiocytes (CD68+). This study showed that the inflammatory infiltrate mainly consists of histiocytes and multinucleated giant cells, in addition to the presence of a large number of fungal cells. The identified inflammatory cells showed the following frequency: CD68+ histiocytes > CD3+ T lymphocytes > CD4+ T > CD8+ T lymphocytes > CD57+ natural killer cells > CD79+ plasma cells > CD20+ B lymphocytes. Based on the findings of a large number of fungal cells in the infected tissues and the disorganized cell arrangement in the granuloma, we hypothesize that patients with Jorge Lobo's disease present immunoregulatory disturbances, which are likely to be specific and perhaps responsible for the lack of containment of the pathogen.
authors.affiliation: Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru-SP, Brazil. imunologia@ilsl.br
authors.forename: Fátima Regina
authors.initials: FR
authors.lastname: Vilani-Moreno
authors.forename: Andréa Faria Fernandes
authors.initials: AF
authors.lastname: Belone
authors.forename: Cleverson Teixeira
authors.initials: CT
authors.lastname: Soares
authors.forename: Diltor Vladimir Araújo
authors.initials: DV
authors.lastname: Opromolla
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D001938:Brazil; D003881:Dermatomycoses / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D015726:Giant Cells / Q000473:pathology*; D006099:Granuloma / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D006790:Host-Parasite Interactions; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology; D016131:Lymphocyte Subsets / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D003904:Mitosporic Fungi / Q000276:immunology / Q000302:isolation & purification*; D020036:Onygenales / Q000276:immunology / Q000302:isolation & purification*; D010950:Plasma Cells / Q000473:pathology*; D012867:Skin / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000473:pathology
chemical_list: D015703:Antigens, CD