Molecular phylogenetic analysis of Iranian HDV complete genome.
Hepatitis D (delta) virus (HDV) is a subviral pathogen agent and a satellite of Hepatitis B virus. Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, the entire nucleotide sequence of an HDV isolate from an Iranian patient (IR-1) was obtained using twelve pairs of primers to amplify six overlapping fragments covering the whole HDV genome by RT-nested PCR. Phylogenetic and pairwise alignments were done on this new isolate to determine IR-1 position among other isolates. Our results indicate that IR-1 contains 1676 nucleotides encoding 214 a.a. of the hepatitis delta antigen (HDAg). This new isolate belongs to genotype I with most sequence similarity to an Italian HDV isolate (92.6%). At amino acid level, predicted HDAg sequence of IR-1 revealed the most homology with those of Italian and Lebanese isolates. Data analysis confirmed genetic variability and heterogeneity of the HDV species isolated from different geographical areas.
Behzadian
Farida
F
Department of Virology, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran.
Sabahi
Farzaneh
F
Karimi
Mohsen
M
Sadeghizadeh
Majid
M
Maghsoudi
Nader
N
Forooshani
Ramin Sarrami
RS
Shahinsaz
Leila
L
D000328:Adult; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D003699:Hepatitis D / Q000821:virology*; D003698:Hepatitis Delta Virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007492:Iran; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology
D000956:Antigens, Viral; D012367:RNA, Viral