[Experimental study of the effect of new bone formation on new type artificial bone composed of bioactive ceramics].
OBJECTIVE
To investigate the osteogenic potential of four kinds of new bioactive ceramics combined with bovine bone morphogenetic proteins (BMP) and to explore the feasibility of using compounds as bone substitute material.


METHODS
Ninety-six rats were divided into 4 groups (24 in each group). BMP was combined with hydroxyapatite (HA), tricalcium phosphate (TCP), fluoridated-HA (FHA), and collagen-HA(CHA) respectively. The left thighs of the rats implanted with HA/BMP, TCP/BMP, FHA/BMP, and CHA/BMP were used as experimental groups. The right thighs of the rats implanted with HA, TCP, CHA, and decalcified dentin matrix (DDM) were used as control groups. The rats were sacrificed 1, 3, 5 and 7 weeks after implantation and bone induction was estimated by alkaline phosphatase (ALP), phosphorus (P), and total protein (TP) measurement. The histological observation and electronic microscope scanning of the implants were also made.


RESULTS
The cartilage growth in the 4 experimental groups and the control group implanted with DDM was observed 1 week after operation and fibrous connective tissues were observed in the other 3 control groups. 3 weeks after implantation, lamellar bone with bone marrow and positive reaction in ALP stain were observed in the 4 experimental groups. No bone formation or positive reaction in ALP stain were observed in the control groups. The amount of ALP activity, P value, and new bone formation in the experimental groups were higher than those in the control group(P < 0.05). The amount of ALP activity, P value, and new bone formation in TCP/BMP group were higher than those in HA/BMP, CHA/BMP and FHA/BMP groups (P < 0.05). There was no significant difference in TP between the BMP treatment group and the control groups. From 5th to 7th week, new bone formation, histochemistry evaluation, and the level of ALP, P, TP value were as high as those in the 3rd week.


CONCLUSIONS
New composite artificial bone of TCP/BMP, HA/BMP, CHA/BMP, and FHA/BMP all prove to be effective, but TCP/BMP is the most effective so that it is the most suitable biomaterial replacement of tissue.
Zhu
Minghua
M
Sichuan Diseases Control and Prevention, Chengdu Sichuan 610041, PR China. xx_ff@163.com
Zeng
Yi
Y
Sun
Tao
T
Peng
Qiang
Q
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology; D002417:Cattle; D002516:Ceramics / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology; D005260:Female; D005269:Femur / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D010758:Phosphorus / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D013848:Thigh; D013997:Time Factors
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011506:Proteins; D010758:Phosphorus; D017886:Durapatite; D000469:Alkaline Phosphatase; C018392:tricalcium phosphate