Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry.
The availability of genome sequence offers the opportunity to further expand our knowledge about proteins expressed by Rickettsia conorii, strictly intracellular bacterium responsible for Mediterranean spotted fever. Using two-dimensional polyacrylamide gel electrophoresis combined with MALDI-TOF mass spectrometry, we established the first reference map of R. conorii proteome. This approach also allowed identification of GroEL as the major antigen recognized by rabbit serum and sera of infected patients. Altogether, this work opens the way to characterize the proteome of R. conorii, to compare protein profiles of different isolates or of bacteria maintained under different experimental conditions and to identify immunogenic proteins as potential vaccine targets.
Renesto
Patricia
P
Unité des Rickettsies, CNRS UMR 6020, IFR-48, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille, France. patricia.renesto@medecine.univ-mrs.fr
Azza
Saïd
S
Dolla
Alain
A
Fourquet
Patrick
P
Vestris
Guy
G
Gorvel
Jean-Pierre
JP
Raoult
Didier
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis*; D018834:Chaperonin 60 / Q000032:analysis / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020543:Proteome / Q000032:analysis*; D011817:Rabbits; D020114:Rickettsia conorii / Q000737:chemistry* / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D020543:Proteome