title: Development and characterization of a recombinant chicken single-chain Fv antibody detecting Eimeria acervulina sporozoite antigen.
abstract: Chicken monoclonal antibody (mAb), 8C3, which is reactive with a sporozoite antigen of Eimeria acervulina, is a potential therapeutic agent against avian coccidiosis caused by Eimeria spp. However, production of large amounts of 8C3 mAb in cell culture system is labor intensive and not cost-effective. Accordingly, recombinant single chain variable fragment (ScFv) antibody was constructed by amplification of the V(H) and V(L) genes from chicken hybridoma, 8C3 and when expressed in E. coli gave 5 mg l(-1). The expressed protein showed antigen binding activity equivalent to that of the parental mAb. In addition, nucleotide sequence comparison of 8C3 gene to the germline chicken V(L) genes suggested that the gene conversion with (V)lambda pseudogenes might contribute to the diversification of V(L) genes in chickens.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Changwon National University, Changwon 641-773, Korea.
authors.forename: Kyung Je
authors.initials: KJ
authors.lastname: Park
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Park
authors.forename: Chun Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Beom Ku
authors.initials: BK
authors.lastname: Han
authors.forename: Tae Sub
authors.initials: TS
authors.lastname: Park
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
authors.forename: Hyun Soon
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Jin-Kyoo
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D004539:Eimeria / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D007167:Immunotherapy / Q000379:methods; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000517:prevention & control; D011994:Recombinant Proteins / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D034101:Sporozoites / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D018076:DNA, Complementary; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins