Platelet-rich plasmas: growth factor content and roles in wound healing.
Platelet-rich plasmas (PRPs) are used in a variety of clinical applications, based on the premise that higher growth factor content should promote better healing. In this study, we have determined the effects of calcium and thrombin on the release of EGF, TGF-alpha, IGF-1, Ang-2 and IL-1beta from PRPs, and assessed the mitogenic potential of PRP supernatants on osteoblast and endothelial cell division. ELISA assays indicate that (i) mean growth factor concentrations vary from traces (TGF-alpha) to 5.5 ng/mL (IGF-1), (ii) there are significant variations in growth factor concentrations between individuals, and (iii) calcium and thrombin regulate growth factor release, synthesis, and/or degradation in stereotyped patterns that are specific to each growth factor. PRP supernatants promote strong osteoblast and endothelial cell divisions, supporting the concept that PRPs may be beneficial in wound healing.


BACKGROUND
PRPs, platelet-rich plasmas; GFs, growth factors; EGF, epidermal growth factor; TGF-alpha, transforming growth factor-alpha; IGF-1, insulin-like growth factor-1; Ang-2, angiopoietin-2; IL-1beta, interleukin-1 beta; HUVECs, human umbilical vein endothelial cells; hFOB 1.19, human fetal osteoblasts; and FBS, fetal bovine serum.
Fréchette
J-P
JP
Faculté de médecine dentaire, Université Laval, Québec, Province Québec, Canada G1K 7P4.
Martineau
I
I
Gagnon
G
G
D042702:Angiopoietin-2 / Q000032:analysis; D001792:Blood Platelets / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000032:analysis; D006133:Growth Substances / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D010006:Osteoblasts / Q000187:drug effects; D010949:Plasma; D013917:Thrombin / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
D042702:Angiopoietin-2; D006133:Growth Substances; D007375:Interleukin-1; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D013917:Thrombin; D002118:Calcium