title: Role of the N-linked glycans of the prM and E envelope proteins in tick-borne encephalitis virus particle secretion.
abstract: The tick-borne encephalitis (TBE) virus has two membrane glycoproteins (prM and E), which each has one N-linked glycan. Constructs that express prM and E proteins of TBE virus have been shown to produce virus-like particles (VLPs), which have surface properties that are similar to those of infectious viruses. To reveal the function of glycosylation of the TBE virus prM and E proteins in the secretion of VLPs, we expressed glycosylation-mutated prM and E proteins and compared the secretion levels and biological properties of the VLPs. In the prM protein glycosylation-deficient mutant, the level of secreted E protein was reduced to 60% of the wild-type level. On the other hand, in the E or prM-E protein glycosylation-deficient mutant, the level of secreted E protein was reduced to 10% of the wild-type level. Furthermore, the mutant which was glycosylated at positions 66 and 154 in protein E, the level of secreted E protein was four-fold higher than that of the wild-type. However, in the mutant which was glycosylated at position 66 only, E protein secretion was reduced to only 10% of the wild-type level. These data suggest that the glycan associated with the N-linked glycosylation site at position 154 in protein E plays an important role in VLP secretion.
authors.affiliation: Laboratory of Public Health, Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Goto
authors.forename: Kentarou
authors.initials: K
authors.lastname: Yoshii
authors.forename: Mayumi
authors.initials: M
authors.lastname: Obara
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Ueki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Takashima
mesh_terms: D004669:Encephalitis Viruses, Tick-Borne / Q000502:physiology*; D006031:Glycosylation; D006801:Humans; D017510:Protein Folding; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000502:physiology*; D014771:Virion / Q000502:physiology*
chemical_list: D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus; C065696:glycoprotein E, Flavivirus