[Establishment of plantlet regeneration system of medicinal plant Aconitum coreanum].
OBJECTIVE
To set up the rapid propagation and plantlet regeneration system of medicinal plant Aconitum coreanum (Levi.) Rpaics.


METHODS
Adventitious buds and regenerated plantlet were obtained by shoot tip culture through different media and combination of plant growth regulators.


RESULTS
MS medium was the optimal medium for differentiation and proliferation of shoot tip. MS medium with 6-BA 2 to approximately 5 mg/L was found to be most suitable for the induction of bud. MS with 6-BA 2 mg/L + GA3 1 to approximately 5 mg/L or adenine 1 to approximately 5 mg/L was best combination for the growth and proliferation of adventitious buds, and MS with IBA 0.5 to approximately 1 mg/L for the induction of root.


CONCLUSIONS
Tip culture may be used as a useful method to get regenerated plantlet of Aconitum coreanum under certain conditions.
Xu
Xiuquan
X
College of Biological and Environmental Sciences, Jiangsu University, Zhenjiang.
Yu
Rongmin
R
Zhao
Yu
Y
D031841:Aconitum / Q000254:growth & development*; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators