title: [Study of solid-phase time-resolved fluorescence label immunoassay].
abstract: This paper describes optimal conditions for HBsAbIgG labeling with a new fluorescence probe, 4,7-bis-chorosulfophenyl-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) for the solid phase time-resolved fluorimmunoassay (TRFIA). The result of experiment under states clearly that BCPDA may react with protein under relative mild condition. The relative bioactivity of reacted protein was more than 80%. The labeling molar ratio of BCPDA for HBsAbIgG was 45-70. The recovery was higher than 80%. Protein-BCPDA-Eu3+ complex is stable. It can emit very high fluorescence intensity with very long fluorescence life times. The fluorescence of Protein-BCPDA-Eu3+ complex has a very large stokes shift (270 nm). The emission band at 611.2 nm is very narrow. The research provides the base for developing non-isotopic immunoassay technique and clinical medical diagnosis.
authors.affiliation: National Analytical Research Center of Electrochemistry and Spectroscopy, Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Pan
authors.forename: Si-hong
authors.initials: SH
authors.lastname: Zhou
authors.forename: Wen-wei
authors.initials: WW
authors.lastname: Sun
authors.forename: Wen-bing
authors.initials: WB
authors.lastname: Xie
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay / Q000379:methods*; D010618:Phenanthrolines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C519844:1,10-phenanthroline-2,9-dicarboxylic acid; D005456:Fluorescent Dyes; D010618:Phenanthrolines; C025205:1,10-phenanthroline