Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines.
Monoclonal antibody MAb-8 was evaluated for detection of human metapneumovirus (HMPV) in shell vial centrifugation cultures (SVCC). Detection of HMPV was similar in A549, HEp-2, and LLC-MK2 SVCC, and MAb-8 staining was optimal on day 2 postinoculation. Availability of SVCC for HMPV will be of significant benefit to clinical laboratories.
Landry
Marie L
ML
Department of Laboratory Medicine, Yale University School of Medicine, P.O. Box 208035, New Haven, CT 06520-8035, USA. marie.landry@yale.edu
Ferguson
David
D
Cohen
Sandra
S
Peret
Teresa C T
TC
Erdman
Dean D
DD
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D002460:Cell Line; D002498:Centrifugation; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007223:Infant; D029121:Metapneumovirus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D013194:Staining and Labeling / Q000379:methods*; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral