Production of piglets with sexed semen employing a non-surgical insemination technique.
The aim of the present study was to ascertain whether multiparous sows could successfully be inseminated with sexed semen non-surgically. Spermatozoa were stained with Hoechst 33342 and separated flowcytometrically in X- and Y-chromosome bearing sperm populations employing the Beltsville Sperm Sexing Technology (BSST). After weaning, estrus was induced in sows with PMSG and hCG. Animals were inseminated once per estrus non-surgically with a specially designed catheter into the tip of the uterine horn, employing 50x10(6) of either sexed or non-sexed spermatozoa diluted in 2 ml Androhep. Pregnant sows were allowed to go to term. Mean pregnancy rate from inseminations with unsexed spermatozoa was 54.5% whereas inseminations with sexed spermatozoa resulted in 33.3% pregnant sows. All but one piglet born after insemination with sexed semen were of the predicted sex. The sex of those piglets born after inseminations with non-sexed spermatozoa was 61.1% for male and 38.9% for female sex. It is concluded that non-surgically inseminations with flowcytometrically sexed spermatozoa can be conducted successfully.
Grossfeld
R
R
Department of Biotechnology, Institute of Animal Breeding, Mariensee (FAL), Hoeltystr. 10, 31535 Neustadt, Germany.
Klinc
P
P
Sieg
B
B
Rath
D
D
D000818:Animals; D001562:Benzimidazoles; D001724:Birth Weight; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D012732:Sex Determination Analysis / Q000662:veterinary*; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*; D013552:Swine; D014960:X Chromosome; D014998:Y Chromosome
D001562:Benzimidazoles; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride