[Human hyperplasic suppress gene (hHSG) could increase the chemotherapy sensitivity of human tumor cells in vitro].
OBJECTIVE
To investigate whether the hHSG can increase the chemotherapy sensitivity of two human tumor cell lines,lung cancer cell line (A549) and cervical cancer cell line (HeLa S3), in which the hHSG expression levels are different.


METHODS
After detecting the hHSG expression in different tumor cell lines with immunohistochemistry; we selected the A549 with relatively low expression of hHSG and HeLa S3 with high expression. After these two cell lines were transfected with recombinant eukaryotic expression vector of pEGFP-hHSG and pEGFP by electroporation respectively,and cultured for 24 h, the CHX was added to the medium, and the impacts of hHSG on chemotherapy sensitivity were evaluated by cell counting and MTT assay in the following days.


RESULTS
hHSG expressed at different levels in all investigated tumor cell lines. Exogenetic hHSG inhibited the proliferation of tumor cell lines, and increased their sensitivity for CHX significantly.


CONCLUSIONS
hHSG gene can inhibit the proliferation of tumor cells and increase their chemotherapy sensitivity despite of its endogenetic expression levels.
Wang
Ping
P
Peking University Center for Human Disease Genomic, Beijing 100083, China.
Wu
Li-na
LN
Jiang
Chun-sun
CS
Li
Zhi-xin
ZX
Zhang
Ying-mei
YM
Chen
Guang-hui
GH
Qiu
Xiao-yan
XY
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018274:Electroporation; D016147:Genes, Tumor Suppressor; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
D000970:Antineoplastic Agents