title: Characterization of the bovine ampkgamma1 gene.
abstract: AMP-activated protein kinase (AMPK) represents the mammalian form of the core component of a kinase cascade that is conserved between fungi, plants, and animals. AMPK plays a major role in protecting mammalian cells from metabolic stress by switching off biosynthetic pathways that require ATP and switching on ATP-regenerating pathways. In this report, we describe the isolation and characterization of the gene for the noncatalytic bovine gamma1 subunit of AMPK. The bovine ampkgamma1 (PRKAG1) gene spans in excess of 14 kb and is located at BTA 5q21-q22. It consists of 12 exons ranging in size from 38 b to 166 b, interspersed with 11 introns that range between 97 b and 6753 b in length. The coding region of the bovine gene shares 93% and 90% nucleotide sequence similarity with its human and rat counterparts, and the bovine AMPKgamma1 protein is 98% and 95% identical to its human and rat homologs, respectively, in amino acid sequence. SNP discovery using a cattle DNA panel revealed a number of polymorphisms that may be useful for the evaluation of ampkgamma1 as a candidate gene for energy metabolism-related production traits.
authors.affiliation: Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada, T1J 4B1. benkelb@agr.gc.ca
authors.forename: Bernhard
authors.initials: B
authors.lastname: Benkel
authors.forename: Sonja
authors.initials: S
authors.lastname: Kollers
authors.forename: Ruedi
authors.initials: R
authors.lastname: Fries
authors.forename: Alexei
authors.initials: A
authors.lastname: Sazanov
authors.forename: Erin
authors.initials: E
authors.lastname: Yoshida
authors.forename: Edith
authors.initials: E
authors.lastname: Valle
authors.forename: Jon
authors.initials: J
authors.lastname: Davoren
authors.forename: Donal
authors.initials: D
authors.lastname: Hickey
mesh_terms: D055372:AMP-Activated Protein Kinases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment
chemical_list: D009097:Multienzyme Complexes; D004247:DNA; C526222:PRKAG1 protein, human; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases