title: BAC-based PCR fragment microarray: high-resolution detection of chromosomal deletion and duplication breakpoints.
abstract: The introduction of molecular techniques in conjunction with classical cytogenetic methods has in recent years greatly improved the diagnostic potential for chromosomal abnormalities. In particular, microarray-comparative genomic hybridization (CGH) based on the use of BAC clones promises a sensitive strategy for the detection of DNA copy-number changes on a genomewide scale, offering a resolution as high as >30,000 "bands" (as defined by the number of BACs within the currently highest-density BAC array) [Ishkanian et al., 2004]. We have tested the possibility of further increasing this resolution using PCR fragments generated from individual BAC clones. Using this approach, we have efficiently defined the proximal and distal breakpoints in two cytogenetic cases, one duplication and one deletion, to within 5-20 kb. The results support the potential use of BAC-based PCR fragments to further improve the resolution of the microarray-CGH strategy by an order of magnitude.
authors.affiliation: Murdoch Childrens Research Institute, University of Melbourne, Royal Children's Hospital, Parkville, Australia.
authors.forename: Hua
authors.initials: H
authors.lastname: Ren
authors.forename: Wendy
authors.initials: W
authors.lastname: Francis
authors.forename: Amber
authors.initials: A
authors.lastname: Boys
authors.forename: Anderly C
authors.initials: AC
authors.lastname: Chueh
authors.forename: Nick
authors.initials: N
authors.lastname: Wong
authors.forename: Phung
authors.initials: P
authors.lastname: La
authors.forename: Lee H
authors.initials: LH
authors.lastname: Wong
authors.forename: Jacinta
authors.initials: J
authors.lastname: Ryan
authors.forename: Howard R
authors.initials: HR
authors.lastname: Slater
authors.forename: K H Andy
authors.initials: KH
authors.lastname: Choo
mesh_terms: D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods; D023281:Genomics / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*