Effects of some antiviral isatinisothiosemicarbazones on cellular and viral ribonucleic acid synthesis in Mengovirus-infected FL cells.
Three antiviral isatinisothiosemicarbazones strongly inhibited the incorporation of [(3)H]uridine into the ribonucleic acid (RNA) of FL cells as a consequence of the inhibition of uridine transport. After prelabeling of cells at a low temperature (1 h at 16 C) with uptake of [(3)H]uridine into the acid-soluble nucleotide pool, the later addition of the test compounds revealed only a small or negligible influence on host-directed RNA synthesis. The pulse-labeled soluble nucleotide pool of FL cells was sufficient to give a gradual increase in incorporation into RNA over a period of 7 h. With the same method of prelabeling at the beginning of the experiment, it was also possible to detect virus-induced RNA synthesis in the presence of actinomycin D. In this way the specific inhibitory action of the three isatinisothiosemicarbazones on viral RNA synthesis could be demonstrated.
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D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D018188:Cardiovirus Infections / Q000188:drug therapy* / Q000821:virology*; D002478:Cells, Cultured; D003080:Cold Temperature; D003609:Dactinomycin / Q000494:pharmacology; D008574:Mengovirus / Q000187:drug effects* / Q000235:genetics; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D013882:Thiosemicarbazones / Q000494:pharmacology*; D014316:Tritium; D014529:Uridine / Q000493:pharmacokinetics
D000998:Antiviral Agents; D019384:Nucleic Acid Synthesis Inhibitors; D012367:RNA, Viral; D013882:Thiosemicarbazones; D014316:Tritium; D003609:Dactinomycin; D014529:Uridine