Genetic linkage maps of the red flour beetle, Tribolium castaneum, based on bacterial artificial chromosomes and expressed sequence tags.
A genetic linkage map was constructed in a backcross family of the red flour beetle, Tribolium castaneum, based largely on sequences from bacterial artificial chromosome (BAC) ends and untranslated regions from random cDNA's. In most cases, dimorphisms were detected using heteroduplex or single-strand conformational polymorphism analysis after specific PCR amplification. The map incorporates a total of 424 markers, including 190 BACs and 165 cDNA's, as well as 69 genes, transposon insertion sites, sequence-tagged sites, microsatellites, and amplified fragment-length polymorphisms. Mapped loci are distributed along 571 cM, spanning all 10 linkage groups at an average marker separation of 1.3 cM. This genetic map provides a framework for positional cloning and a scaffold for integration of the emerging physical map and genome sequence assembly. The map and corresponding sequences can be accessed through BeetleBase (http://www.bioinformatics.ksu.edu/BeetleBase/).
Lorenzen
Marcé D
MD
U.S. Department of Agriculture-Agricultural Research Service-GMPRC, Manhattan, Kansas 66502, USA.
Doyungan
Zaldy
Z
Savard
Joel
J
Snow
Kathy
K
Crumly
Lindsey R
LR
Shippy
Teresa D
TD
Stuart
Jeffrey J
JJ
Brown
Susan J
SJ
Beeman
Richard W
RW
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D003433:Crosses, Genetic; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000378:metabolism; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005260:Female; D008040:Genetic Linkage; D020407:Internet; D008297:Male; D018895:Microsatellite Repeats; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity; D014231:Tribolium / Q000235:genetics*
D004251:DNA Transposable Elements; D018076:DNA, Complementary; D009692:Nucleic Acid Heteroduplexes; D004247:DNA