Effects of Abeta25-35 on neurogenesis in the adult mouse subventricular zone and dentate gyrus.
It has been demonstrated that neuorgenesis driven by neural precursor cells persists well into the adult period. This study was to observe the effects of Amyloid-beta (25-35) peptide (Abeta(25-35)) on neurogenesis in the subventricular zone and dentate gyrus of adult mouse brain. Aggregated Abeta(25-35)(1 mg/ml, 3 microl) was injected into the lateral ventricle of adult mouse. Animals were transcardially perfused with 4% paraformaldehyde in PBS, respectively at 5, 10, 20, 30 days after the Abeta(25-35) injection. All the animals were injected with BrdU (50 mg/kg, i. p) to label the neural precursor cells 24 h before the each perfusion. NeuN immunofluorescence and BrdU immunohistology were performed. It was found that Abeta(25-35) could injure the mature neurons and decrease the number of NeuN positive neurons. It also showed that Abeta(25-35) inhibited neurogenesis and significantly decreased the number of BrdU positive cells in the dentate gyrus of hippocampus, but it had no obvious effects on neurogenesis in the subventricular zone. The present results indicated that Abeta(25-35) could impair neurogenesis in the hippocampus of adult mouse brain.
Li
Xuekun
X
Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 5# Dong Dan San Tiao, Beijing 100005, China.
Zuo
Pingping
P
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D018891:Dentate Gyrus / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D020547:Lateral Ventricles / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010455:Peptides; C074636:amyloid beta-protein (25-35); D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine