title: Outcome of four weeks' intervention with probiotics on symptoms and endoscopic appearance after surgical reconstruction with a J-configurated ileal-pouch-anal-anastomosis in ulcerative colitis.
abstract: OBJECTIVE
  Pouchitis is a common and troublesome condition in patients operated on with ileal-pouch-anal-anastomosis (IPAA). A disturbed microecology in the pouch has been suggested as one possible explanation. In a previous double-blind, randomized, controlled study we demonstrated clinical improvement of symptoms in patients with ulcerative colitis (UC) operated on with IPAA, during intervention with live probiotic microbes Lactobacilli and Bifidobacteriae. The aim of the present study was to confirm our previous results in a much larger material, including clinical symptoms, faecal flora and endoscopic evaluation, and to compare the results in UC/IPAA patients with those of patients with familial adenomatous polyposis (FAP) with IPAA and UC patients with ileorectal anastomosis (IRA).
  
  
  METHODS
  Five hundred millilitres of a fermented milk product (Cultura) containing live lactobacilli (La-5) and bifidobacteriae (Bb-12) was given daily for 4 weeks to 51 UC patients and 10 patients with FAP, operated on with IPAA, and six UC patients operated on for IRA. Stool samples were cultured for examination of lactobacilli, bifidobacteriae, fungi and pH before, during and after intervention. Before, during and after intervention, endoscopic evaluation was performed. Categorized symptomatology was examined prospectively using diary cards in addition to an interview, before and on the last day of intervention.
  
  
  RESULTS
  The number of lactobacilli and bifidobacteriae increased significantly during intervention in the UC patients operated on with IPAA and remained significantly increased one week after intervention. Involuntary defecation, leakage, abdominal cramps and the need for napkins (category I), faecal number and consistency (category II) and mucus and urge to evacuate stools (category III) were significantly decreased during intervention in the UC/IPAA group. In the FAP group there was a significant decrease in faecal leakage, abdominal cramps and use of napkins (category I) during intervention. The median endoscopic score of inflammation was significantly decreased during intervention in the UC/IPAA patients. Blood tests, faecal fungi and faecal pH did not change significantly during intervention.
  
  
  CONCLUSIONS
  Results of this extended study, showing an effect of probiotics on symptoms and endoscopic inflammation in UC patients operated on with IPAA confirm our previously reported effect of probiotics on clinical symptoms and endoscopic score in a smaller, double-blind, randomized, controlled study. The significantly higher response to probiotics in families with increased risk of IBD will have to be repeated in future studies.
authors.affiliation: Department of Medicine, Rikshospitalet University Hospital, Oslo, Norway. Knut.Laake@Rikshospitalet.no
authors.forename: K O
authors.initials: KO
authors.lastname: Laake
authors.forename: A
authors.initials: A
authors.lastname: Bjørneklett
authors.forename: G
authors.initials: G
authors.lastname: Aamodt
authors.forename: L
authors.initials: L
authors.lastname: Aabakken
authors.forename: M
authors.initials: M
authors.lastname: Jacobsen
authors.forename: A
authors.initials: A
authors.lastname: Bakka
authors.forename: M H
authors.initials: MH
authors.lastname: Vatn
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000601:surgery*; D039021:Colonic Pouches; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007778:Lactobacillus; D008297:Male; D008875:Middle Aged; D019449:Pouchitis / Q000209:etiology / Q000628:therapy*; D019936:Probiotics / Q000627:therapeutic use*; D016737:Proctocolectomy, Restorative / Q000009:adverse effects* / Q000379:methods; D012016:Reference Values; D018570:Risk Assessment; D012720:Severity of Illness Index; D016037:Single-Blind Method; D016896:Treatment Outcome