title: An in vitro study of cytotoxic effects of gossypol on human epidermoid larynx carcinoma cell line (HEp-2).
abstract: OBJECTIVE
  The study was aimed on an evaluation of the effect of gossypol at concentration of 25-150 microM on the level of apoptosis in the human epidermoid larynx carcinoma cell line, HEp-2, in vitro.
  
  
  METHODS
  To determine the apoptotic effects of gossypol on growth of human epidermoid larynx carcinoma cell lines (HEp-2 cells), morphological evaluation of the cells and DNA fragmentation analysis were used.
  
  
  RESULTS
  6 h incubation of HEp-2 cells with gossypol at concentrations of 50 microM and 75 microM resulted in the increase of apoptosis rate by 26 and 23%, respectively.
  
  
  CONCLUSIONS
  This preliminary study may constitute a base for in vivo studies of anticancer properties of gossypol.
authors.affiliation: Department of Medical Biology and Genetics, Gazi University, Faculty of Medicine, Besevler, Ankara, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Konac
authors.forename: Abdullah
authors.initials: A
authors.lastname: Ekmekci
authors.forename: Erkan
authors.initials: E
authors.lastname: Yurtcu
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Ergun
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006072:Gossypol / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D006072:Gossypol