Accelerated recycling of transferrin receptor in Theileria-transformed B cells.
We found that phoshatidylinositol-3 kinase (PI3-K) markedly contributes to the increased surface expression of bovine transferrin receptor (TfR) on Theileria-infected lymphocytes. We observed that all aspects of TfR turnover are upregulated in parasitized B cells and we were able to detect TfR colocalizing with EEA1 (early endosome antigen 1) and Rab11 at the ultrastructure level in Theileria-infected B cells. We demonstrated recycling of TfR through Rab5- and Rab11-positive compartments by transfection of dominant negative guanosine diphosphate (GDP)-on mutants of the GTPases. Therefore, in Theileria-transformed B cells constitutive PI3-K activity leads to accelerated TfR recycling through Rab5- and Rab11-positive compartments.
Baumgartner
Martin
M
URA CNRS 2581, Laboratoire de Signalisation Immunoparasitaire, Département de Parasitologie, Institut Pasteur, Paris Cedex, France.
Chaussepied
Marie
M
Raposo
Graça
G
Goud
Bruno
B
Langsley
Gordon
G
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000469:parasitology / Q000648:ultrastructure; D002417:Cattle; D002451:Cell Compartmentation; D002460:Cell Line; D011992:Endosomes / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism*; D016794:Theileria / Q000502:physiology*; D015854:Up-Regulation; D033921:Vesicular Transport Proteins; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
D008565:Membrane Proteins; D011990:Receptors, Transferrin; D033921:Vesicular Transport Proteins; C093846:early endosome antigen 1; C118779:rab11 binding protein, Bos taurus; D019869:Phosphatidylinositol 3-Kinases; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins