Quantitative three-dimensional microscopy approaches with applications in breast cancer biology including measurement of genomic instability.
Understanding tissue development, tissue homeostasis and what goes wrong in these processes during tumorigenesis, requires knowledge of the kinetics of multiple, molecular pathways in individual cells while cells are in their tissue context. This review outlines progress and future directions necessary in quantitative microscopy for gaining this knowledge, using the mammary gland as a model system.
Lockett
Stephen
S
National Cancer Institute-Frederick/Scientific Applications International Corporation, Frederick, Maryland, USA. slockett@ncifcrf.gov
Ortiz de Solorzano
Carlos
C
Baggett
Daniel
D
Chin
Koei
K
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*