title: [The methylation pattern of LRP15 gene in acute leukemia].
abstract: OBJECTIVE
  To investigate the methylation status of LRP15 gene in acute leukemia (AL) and its role in tumorigenesis.
  
  
  METHODS
  73 cases of AL and 9 healthy subjects as well as COS7, K562 and HL60 cell lines were studied with methylation specific PCR (MSP).
  
  
  RESULTS
  LRP15 was not detected in all the samples. No LRP15 methylation was detected in COS7, but LRP15 was methylated in K562 and HL60. In nearly all of the French-American-British leukemia subtypes, we found that the frequency of LRP15 methylation was 71.2% (52/73) of AL and none in the 9 healthy subjects. The difference in mean methylation for LRP15 between these two kinds of samples is statistically significant (P < 0.05). Hypermethylation of the LRP15 gene was found in 57.1% (16/28) of the newly diagnosed AL and 83.3% of the relapsed AL respectively; this is also statistically significant (P < 0.05).
  
  
  CONCLUSIONS
  LRP15 methylation change is a common abnormality in leukemia and LRP15 is postulated to be a tumor suppressor gene.
authors.affiliation: Department of Hematology, PLA General Hospital, Beijing 100853, China. lipingdou@yahoo.com.cn
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Dou
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Qing
authors.initials: Q
authors.lastname: Lü
authors.forename: Zhou-min
authors.initials: ZM
authors.lastname: Xu
authors.forename: Hai-chuan
authors.initials: HC
authors.lastname: Liu
authors.forename: Fang-ding
authors.initials: FD
authors.lastname: Lou
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D019556:COS Cells; D019175:DNA Methylation; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D011506:Proteins / Q000235:genetics*
chemical_list: C477813:LRRC3B protein, human; D009363:Neoplasm Proteins; D011506:Proteins