Identification of expressed genes in regenerating rat liver in 0-4-8-12 h short interval successive partial hepatectomy.
OBJECTIVE
To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.


METHODS
Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.


RESULTS
One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression. Eighty-six genes were unreported, associated with liver regeneration (LR).


CONCLUSIONS
Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar, but the expression of 43 genes is specifically altered in SISPH.
Xu
Cun-Shuan
CS
College of Life Science, Henan Normal University, Xinxiang 453002, Henan Province, China. xucs@x263.net
Yuan
Jin-Yun
JY
Li
Wen-Qiang
WQ
Han
Hong-Peng
HP
Yang
Ke-Jin
KJ
Chang
Cui-Fang
CF
Zhao
Li-Feng
LF
Li
Yu-Chang
YC
Zhang
Hui-Yong
HY
Rahman
Salman
S
Zhang
Jing-Bo
JB
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D006498:Hepatectomy; D008099:Liver / Q000502:physiology* / Q000601:surgery; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors