Carbohydrate localization on Gyrodactylus salaris and G. derjavini and corresponding carbohydrate binding capacity of their hosts Salmo salar and S. trutta.
The congeners Gyrodactylus salaris and G. derjavini are specific ectoparasites of Atlantic salmon Salmo salar and brown trout S. trutta, respectively. To elucidate the involvement of lectin-carbohydrate interactions in this host specificity, carbohydrates on the tegument of the two species and the corresponding lectin activity of their hosts have been studied. Carbohydrate composition on the tegument differed significantly between the two gyrodactylids. Three of four commercially available peroxidase-labelled lectins with primary affinity towards D-mannoside, D-GalNAc and L-fucose bound more strongly to G. derjavini than to G. salaris. Lectins with an affinity towards D-mannoside and D-GalNAc bound significantly stronger to the cephalic lobes on G. derjavini compared to the tegument and sheaths of the hamuli. One brown trout strain and three different salmon strains were tested for lectin activity in skin and plasma. Two Baltic salmon strains and one strain from the Atlantic region were included. Brown trout differed significantly from the salmon strains when skin samples were tested for D-GalNAc activity. Lectins binding to other carbohydrates showed trends for similar host differences. The implications of carbohydrate-lectin interactions for host specificity in gyrodactylids are discussed.
Jørndrup
S
S
Department of Veterinary Microbiology, Section of Fish Diseases, Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870, Frederiksberg C, Denmark.
Buchmann
K
K
D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D004198:Disease Susceptibility; D005393:Fish Diseases / Q000175:diagnosis / Q000469:parasitology*; D005618:Fresh Water; D006790:Host-Parasite Interactions; D037102:Lectins / Q000378:metabolism; D010274:Parasitology / Q000379:methods; D011485:Protein Binding; D020317:Salmo salar / Q000469:parasitology*; D012867:Skin / Q000378:metabolism; D014200:Trematoda / Q000302:isolation & purification*; D014201:Trematode Infections / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D014337:Trout / Q000469:parasitology*
D002241:Carbohydrates; D037102:Lectins