Effect of certain polypeptides on the biological activities of Measles virus.
The effect of a selected tripeptide, Z-d-Phe-Phe-Arg(NO(2)) (SV-4814) on the kinetics of lysis of green monkey erythrocytes by measles virus hemolysin suggested conditions of competitive inhibition. SV-4814 prevented not only hemoglobin release, but also the preceding leakage of K(+) through the cell membrane. The capacity of 12 different dipeptides, 6 tripeptides, including SV-4814, and 4 tetrapeptides to inhibit measles virus-specific hemolysis and infectivity was analyzed. Tri- and tetrapeptides were more active than dipeptides. The presence of aromatic amino acids and a free carboxyl terminal appeared to be a prerequisite for inhibitory activity. Two compounds, one tripeptide (Z-Phe-d-Phe-d-Tyr) and one tetrapeptide [Z-d-Phe-Arg(NO(2))-d-Met], carried an inhibitory activity, which was about two times higher than that of SV-4814.
Norrby
E
E
Sievertsson
H
H
D000818:Animals; D002522:Chlorocebus aethiops; D004912:Erythrocytes / Q000378:metabolism / Q000821:virology; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D008457:Measles / Q000188:drug therapy* / Q000821:virology*; D008459:Measles virus / Q000187:drug effects* / Q000472:pathogenicity; D010455:Peptides / Q000494:pharmacology*; D014709:Vero Cells; D014774:Virulence / Q000187:drug effects
D006454:Hemoglobins; D010455:Peptides