title: Functional analysis of cells obtained from bronchoalveolar lavage fluid (BALF) of lung cancer patients.
abstract: BALF from tumor segments provides access to immune system cells in contact with lung tumors. We analyzed BALF cells as to their production of H2O2 and NO, comparing tumor-affected to non-affected lung segments. Twelve patients were studied (4 NSCLC, 3 SCC, 5 Adenocarcinoma). The cell numbers recovered from BALF varied, and, in adenocarcinoma patients, smaller numbers were recovered from tumor-affected segments. H2O2 production (up to 6.3 nmoles/2x10(5)cells) was obtained in 7/12 patients and, in these, it was more frequent in non-affected segments (7/7) than in affected segments (2/7). After culture, NO production was observed in three patients (6 to 314 microM) that also produced H2O2. These functional characteristics of cells in contact with neoplasia may have a role in determining the fate of the interactions between the immune system and lung cancer.
authors.affiliation: Departamento de Imunologia Instituto de Ciências Biomédicas-USP, Av Prof Lineu Prestes 1730 - CEP 05508-000 - Cidade Universitária - São Paulo - SP - Brazil.
authors.forename: Luciene Barbosa
authors.initials: LB
authors.lastname: Anselmo
authors.forename: Jefferson Luiz
authors.initials: JL
authors.lastname: Gross
authors.forename: Fabio
authors.initials: F
authors.lastname: Haddad
authors.forename: Daniel
authors.initials: D
authors.lastname: Deheinzelin
authors.forename: Riad Naim
authors.initials: RN
authors.lastname: Younes
authors.forename: José Alexandre Marzagão
authors.initials: JA
authors.lastname: Barbuto
mesh_terms: D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D006861:Hydrogen Peroxide