Interactions between hen egg-white lysozyme, PEG2,000, and PLA50 at the air-water interface.
In this paper, we compared the efficiency of polymer films, made of a poly(ethylene glycol) (PEG2,000)/poly(d,l-lactide) (PLA50) mixture, or a PEG2,000-PLA50 copolymer, to prevent adsorption of a model protein, the hen egg-white lysozyme (HEWL), at the air-water interface. This was achieved by analyzing the surface pressure/surface area curves, and the X-ray reflectivity data of the polymer films spread on a Langmuir trough, obtained in absence or in presence of the protein. For both the mixture and the copolymer, the amount of protein adsorbed at the air-water interface decreases when the density of the polymer surface coverage increases. It was shown that even in a condensed state, the polymer film made by the mixture can not totally prevent HEWL molecules to adsorb and penetrate the polymer mixed film, but however, protein molecules would not be directly exposed to the more hydrophobic phase, i.e. the air phase. It was also shown that the configuration adopted by the copolymer at the interface in its condensed state would prevent adsorption of HEWL molecules for several hours; this would be due in particular to the presence of PEG segments in the interfacial film.
Malzert-Fréon
A
A
Laboratoire de Pharmacie Galénique, UFR de Sciences Pharmaceutiques, bd Becquerel, 14032 Caen, France.
Abillon
O
O
Proust
J E
JE
Gref
R
R
Benoît
J P
JP
Boury
F
F
D000327:Adsorption; D000388:Air; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002645:Chickens; D003102:Colloids / Q000737:chemistry*; D004529:Egg White; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry*; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011312:Pressure; D011506:Proteins / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014965:X-Rays
D003102:Colloids; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D014867:Water; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D009113:Muramidase