title: [The effect of kanglaite injection(KLT) on the proliferation and telomerase activity of rat mesangial cells].
abstract: OBJECTIVE
  To observe the effect of Kanglaite injection(KLT) on the proliferation and telomerase activity of mesangial cells in rats.
  
  
  METHODS
  MTT, telomere repeat amplification protocal (TRAP), ELISA, PAGE and silver-stain were applied to detect the growth rate and telomerase activity of MC after stimulation of KLT and IL-1.
  
  
  RESULTS
  The growth rate of MC was enhanced by IL-1 stimulation, which was accompanied with a redection of the activity of telomerase. Adversely, the growth rate of MC was reduced by KLT, which was accompanied with an enhancement of activity of telomerase. Moreover, the growth rate of MC and the activity of telomerase were both inhibited by the combinative use of IL-1 and KLT without any influence from the sequence of their administration.
  
  
  CONCLUSIONS
  KLT could inhibit proliferation and telomerase activity of MC with or without pre-stimulation with IL-1. KLT might be useful to prevent and treat glomerular nephritis related to MC proliferation.
authors.affiliation: Second Affiliated Hospital, The Medical College of Zhejiang University, Hangzhou 310009, China. hy-doctor@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.forename: Wei-Kun
authors.initials: WK
authors.lastname: Gong
authors.forename: Ze-Feng
authors.initials: ZF
authors.lastname: Xu
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zou
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D031742:Coix / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology*; D007267:Injections; D010938:Plant Oils / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D012639:Seeds / Q000737:chemistry; D019098:Telomerase / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D010938:Plant Oils; D019098:Telomerase