The highly conserved tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex and is required for the G2/M phase transition in the yeast Saccharomyces cerevisiae.
Here we show that the Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p interacts with the origin recognition complex in vivo and in vitro and that overexpression of hemagglutinin-Thg1p selectively impedes growth of orc2-1(Ts) cells at the permissive temperature. Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast.
Rice
Terri S
TS
Department of Pathology, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
Ding
Min
M
Pederson
David S
DS
Heintz
Nicholas H
NH
D002455:Cell Division; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016195:G2 Phase; D006388:Hemagglutinins / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D051738:Origin Recognition Complex; D012354:RNA, Transfer, His; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D006388:Hemagglutinins; D051738:Origin Recognition Complex; D012354:RNA, Transfer, His; D029701:Saccharomyces cerevisiae Proteins; D009713:Nucleotidyltransferases; C020842:guanylyltransferase