[Mapping of regulatory domain of T-protein from Escherichia coli].
To map the regulatory domain of Escherichia coli T-protein.



Fragmentation cloning was employed in cloning of 11 fragments from T-protein. The regulatory activity of each fragment was determined respectively.



The regulatory domain of T-protein was located in the C-terminal 270 amino acids, which was the same location as PDH domain.



T-protein has no independent regulatory domain.
Zhang
Hui
H
Department of Pharmacy, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
Chen
Shu-qing
SQ
D003001:Cloning, Molecular; D015253:Deoxyribonucleases, Type I Site-Specific / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D050436:Regulatory Elements, Transcriptional / Q000235:genetics
D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D015253:Deoxyribonucleases, Type I Site-Specific; C109830:HsdR protein, E coli