title: Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.
abstract: Oligosaccharyltransferase (OST) catalyzes the cotranslational transfer of high-mannose sugars to nascent polypeptides during N-linked glycosylation in the rough endoplasmic reticulum lumen. Nine OST subunits have been identified in yeast. However, the composition and organization of mammalian OST remain unclear. Using two-dimensional Blue Native polyacrylamide gel electrophoresis/sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, we now demonstrate that mammalian OST can be isolated from solubilized, actively engaged ribosomes as multiple distinct protein complexes that range in size from approximately 500 to 700 kDa. These complexes exhibit different ribosome affinities and subunit compositions. The major complex, OSTC(I), had an apparent size of approximately 500 kDa and was readily released from ribosome translocon complexes after puromycin treatment under physiological salt conditions. Two additional complexes were released only after treatment with high salt: OSTC(II) ( approximately 600 kDa) and OSTC(III) ( approximately 700 kDa). Both remained stably associated with heterotrimeric Sec61alphabetagamma, while OSTC(III) also contained the tetrameric TRAP complex. All known mammalian OST subunits (STT3-A, ribophorin I, ribophorin II, OST48, and DAD1) were present in all complexes. In addition, two previously uncharacterized proteins were also copurified with OST. Mass spectrometry identified a 17 kDa protein as DC2 which is weakly homologous to the C-terminal half of yeast Ost3p and Ost6p. The second protein (14 kDa) was tentatively identified as keratinocyte-associated protein 2 (KCP2) and has no previously known function. Our results identify two potential new subunits of mammalian OST and demonstrate a remarkable heterogeneity in OST composition that may reflect a means for controlling nascent chain glycosylation.
authors.affiliation: Division of Molecular Medicine, Oregon Health and Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97201, USA.
authors.forename: Toru
authors.initials: T
authors.lastname: Shibatani
authors.forename: Larry L
authors.initials: LL
authors.lastname: David
authors.forename: Ashley L
authors.initials: AL
authors.lastname: McCormack
authors.forename: Klaus
authors.initials: K
authors.lastname: Frueh
authors.forename: William R
authors.initials: WR
authors.lastname: Skach
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006602:Hexosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D046912:Multiprotein Complexes; D010179:Pancreas / Q000201:enzymology; D021122:Protein Subunits; D040901:Proteomics; D011691:Puromycin; D000069816:SEC Translocation Channels; D012492:Salts; D017386:Sequence Homology, Amino Acid; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D000069816:SEC Translocation Channels; D012492:Salts; D011691:Puromycin; D006602:Hexosyltransferases; C581362:KRTCAP2 protein, human; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase