title: Continuous fabrication of biocatalyst immobilized microparticles using photopolymerization and immiscible liquids in microfluidic systems.
abstract: We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and hydrogel solutions form emulsions in pressure-driven flow in microchannels at high values of the dimensionless capillary number (Ca). The resultant hydrogel droplets are then polymerized in situ via exposure to 365 nm UV light. This technique allows for the generation of monodisperse particles whose size can be controlled by the regulation of flow rates. In addition, both manufacturing microparticles and immobilizing biocatalysts can be performed simultaneously and continuously.
authors.forename: Won Je
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Jeong Yun
authors.initials: JY
authors.lastname: Kim
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Han
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
authors.forename: Gi Hun
authors.initials: GH
authors.lastname: Seong
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010316:Particle Size; D010777:Photochemistry
chemical_list: D004800:Enzymes, Immobilized; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase