title: Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge.
abstract: Two recombinant vaccines against Theileriaparva, based on a near full-length version of the sporozoite surface antigen p67 (p67(635)), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one in Central Kenya. Vaccination reduced severe ECF by 47% at the coast and by 52% in central Kenya from an average incidence of 0.53+/-0.07 (S.E.) in 50 non-immunised controls to an average of 0.27+/-0.05 in 83 immunised animals. The reduction in severe East Coast fever was similar to that observed in laboratory experiments with p67(635) and p67C. The p67 coding sequence from thirteen T. parva field isolates including seven from vaccinated cattle that were not protected, was 100% identical to the gene on which the recombinant vaccine is based, suggesting a predominantly homologous p67 antigenic challenge. The same parasite isolates were however genetically heterogeneous at several loci other than p67.
authors.affiliation: International Livestock Research Institute, P.O. Box 30709 Nairobi. Kenya.
authors.forename: Antony
authors.initials: A
authors.lastname: Musoke
authors.forename: John
authors.initials: J
authors.lastname: Rowlands
authors.forename: Vishvanath
authors.initials: V
authors.lastname: Nene
authors.forename: John
authors.initials: J
authors.lastname: Nyanjui
authors.forename: Joseph
authors.initials: J
authors.lastname: Katende
authors.forename: Paul
authors.initials: P
authors.lastname: Spooner
authors.forename: Stephen
authors.initials: S
authors.lastname: Mwaura
authors.forename: David
authors.initials: D
authors.lastname: Odongo
authors.forename: Catherine
authors.initials: C
authors.lastname: Nkonge
authors.forename: Samuel
authors.initials: S
authors.lastname: Mbogo
authors.forename: Richard
authors.initials: R
authors.lastname: Bishop
authors.forename: Subhash
authors.initials: S
authors.lastname: Morzaria
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D007114:Immunization; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000469:parasitology*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C114309:p67 antigen, Theileria