[Determination of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma].
OBJECTIVE
To establish a method by high-performance liquid chromatography (HPLC) for determining the concentration of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma.


METHODS
Chromatography was performed on a LiChroCART C18 (250 mm x 4 mm, 5 microm) column with the mobile phase consisting of acetonitrile-NaAC (15:85), the flow rate of 1.0 ml/min, and the detection wavelength of 365 nm. Sample extraction was carried out with ethylacetate.


RESULTS
The linear range of mouse plasma mitomycin C concentration was 0.04-1.00 microg/ml, and the linear equation of Y=16 388X-17.17 (r=0.999 8) was derived.


CONCLUSIONS
This method is very easy to operate and suits the need of perclinical pharmacokinetic studies of mitomycin-magnetic nanoparticles and yields accurate and precise results.
Liu
Wei
W
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lw0913@fimmu.com
Chen
Jian-Hai
JH
Ren
Fei
F
Guo
Yun-Bo
YB
Yan
Xi-Qing
XQ
Liu
Yan-Dong
YD
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004659:Enbucrilate; D008280:Magnetics; D051379:Mice; D016685:Mitomycin / Q000008:administration & dosage / Q000097:blood*; D053758:Nanoparticles
D004337:Drug Carriers; D016685:Mitomycin; D004659:Enbucrilate