Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA.
Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was < 0.5 microg kg(-1) in egg and < 1 microg kg(-1) in muscle. For dinitrocarbanilide, the CCbeta was estimated at < 3 microg kg(-1) in egg and < 10 microg kg(-1) in chicken muscle.
Huet
A-C
AC
Centre d'Economie Rurale, laboratoire d'Hormonologie Animale, Marloie, Belgium.
Mortier
L
L
Daeseleire
E
E
Fodey
T
T
Elliott
C
C
Delahaut
P
P
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002224:Carbanilides / Q000032:analysis; D002645:Chickens; D003049:Coccidiostats / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D009132:Muscles / Q000737:chemistry*; D009528:Nicarbazin / Q000032:analysis* / Q000276:immunology; D010880:Piperidines; D011799:Quinazolines / Q000032:analysis* / Q000276:immunology; D052999:Quinazolinones; D015203:Reproducibility of Results
D000906:Antibodies; D002224:Carbanilides; D003049:Coccidiostats; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; D009528:Nicarbazin; C010176:halofuginone