Immunocytochemical detection and reverse transcription polymerase chain reaction expression of oncostatin M (OSM) and its receptor (OSM-Rbeta) in human fetal and adult ovaries.
OBJECTIVE
To investigate the immunocytochemical expression and presence of mRNA transcripts of oncostatin M (OSM) and its exclusive receptor (OSM-Rbeta) in ovaries from human adults and fetuses.


METHODS
Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study.


METHODS
Major tertiary care and referral academic centers.


METHODS
Ten women and girls undergoing laparoscopic ovarian biopsy and 30 women undergoing second-trimester and third-trimester pregnancy terminations.


METHODS
None.


METHODS
Microscopic morphometric analysis, immunocytochemistry for OSM and OSM-Rbeta, and RT-PCR analyses.


RESULTS
There was strong to moderate immunocytochemical staining for OSM in both oocytes and granulosa cells of follicles from primordial stages onwards in ovaries from both fetuses and adults/adolescents. OSM-Rbeta was detected mainly in the oocytes. Transcripts of OSM and OSM-Rbeta RNA were detected by RT-PCR analyses.


CONCLUSIONS
The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles.
Abir
Ronit
R
Infertility and IVF Unit, Department of Obstetrics and Gynecology, Rabin Medical Center, Beilinson Campus, Petah Tikva 49100, Israel. ronita@clalit.org.il
Ao
Asangla
A
Jin
Shaoguang
S
Barnett
Miriam
M
Van den Hurk
Robert
R
Freimann
Sarit
S
Fisch
Benjamin
B
D000293:Adolescent; D000328:Adult; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D053683:Oncostatin M; D006080:Ovarian Follicle / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism*; D053677:Receptors, Oncostatin M; D020133:Reverse Transcriptase Polymerase Chain Reaction
C508351:OSM protein, human; D010455:Peptides; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053677:Receptors, Oncostatin M; D053683:Oncostatin M