[In vivo effect of endometria's and human cervix's secretions on the spermatozoid].
OBJECTIVE
To demonstrate the capacitance stages acquired by the spermatozoa after the in vivo contact with the endometrium and the human cervical canal.


METHODS
Experimental, prospective, and longitudinal.


METHODS
Spermatozoa obtained from the cervix and endometrium, plus post-capacitated semen in a 1 and 4 hours process, from couples that have proved fertility, were processed to evaluate motility, morphology, vitality and identification of morphological patterns with chlortetracycline and then inducted to acrosomal reaction with ionophore A23187.


RESULTS
A safe, fast and harmless technique to obtain endometrial secretions is described. The spermatozoa capacitated in vitro and in vivo developed specific capacitance stages, applying Perry's patterns with the CTC test. Spermatozoa capacitated in vitro and then recovered in the cervix responded favorably to the induction of the acrosomal reaction with calcium ionophore A23187, differing from the obtained with endometrium wash.


CONCLUSIONS
The spermatozoa recovered from the endometrium presented a more advanced capacitance stage than that obtained from the cervix, this is demonstrated with the induction of the acrosomal reaction and the CTC test. These findings suggest that endometrial and cervical tissues promote capacitance, different in each one of them and progressive in their biological route.
Merino Ruiz
Martha
M
Departamento de Ginecología y Obstetricia, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, NL. merino_ruiz@yahoo.com.mx
Luna Aranda
Hilda Magdalena
HM
García Flores
Rogelio
R
Vidal Gutiérrez
Oscar
O
Morales Martínez
Felipe Arturo
FA
Sordia Hernández
Luis
L
Saldívar Rodríguez
Donato
D
Vázquez Méndez
Juana
J
D000328:Adult; D002584:Cervix Uteri / Q000378:metabolism* / Q000502:physiology; D004717:Endometrium / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D011446:Prospective Studies; D013075:Sperm Capacitation / Q000502:physiology*