Improved production of L-lysine by disruption of stationary phase-specific rmf gene in Escherichia coli.
Growth and rate, at which fermentation products are formed in cells, generally decreases during the stationary phase as a result of changes in gene expression. We focused on the rmf gene, which encodes the ribosome modulation factor protein, as a target for strain modification in order to improve the rate of L-lysine production in Escherichia coli. Increased expression of the rmf gene during the stationary phase was confirmed under various cultivation conditions using DNA macroarray analysis. Mutants with disrupted rmf were then generated from an L-lysine-producing E. coli strain. The rates of L-lysine accumulation and production were significantly increased in disruptants that were cultivated with excess phosphate. By contrast, a higher biomass was generated in disruptants that were grown under limited phosphate conditions. These results demonstrate that disruption of the rmf gene significantly affects L-lysine production and growth in E. coli.
Imaizumi
Akira
A
Advanced Technology Department, Fermentation and Biotechnology Laboratories, Global Foods and Amino Acids Company, Ajinomoto Co., Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan. akira_imaizumi@ajinomoto.com
Takikawa
Rie
R
Koseki
Chie
C
Usuda
Yoshihiro
Y
Yasueda
Hisashi
H
Kojima
Hiroyuki
H
Matsui
Kazuhiko
K
Sugimoto
Shin-Ichi
S
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D008239:Lysine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000502:physiology*
D029968:Escherichia coli Proteins; D010710:Phosphates; D012269:Ribosomal Proteins; C063545:ribosome modulation factor, E coli; D008239:Lysine