title: Modified multiplex PCR for identification of Bacillus Calmette-Guérin substrain Tokyo among clinical isolates.
abstract: When an adverse reaction occurs and a mycobacterial species is isolated from a person vaccinated with Bacillus Calmette-Guérin (BCG) or a patient receiving BCG immunotherapy, it is essential to identify whether the isolate is BCG or another mycobacterial species. However, differentiation of BCG from other members of Mycobacterium tuberculosis complex has been very difficult. Using several specific primer-pairs, Bedwell et al. [Bedwell J, Kairo SK, Behr MA, Bygraves JA. Identification of substrains of BCG vaccine using multiplex PCR. Vaccine 2001; 19: 2146-51] recently reported that they could distinguish BCG substrains. We modified their method to improve differentiation of Tokyo 172 from other members of the M. tuberculosis complex, and examined whether this modified method could be applied to clinical isolates. Our method clearly identified BCG substrain (BCG Tokyo 172) among clinical isolates and easily distinguished between M. tuberculosis and wild-type Mycobacterium bovis.
authors.affiliation: Japan BCG Laboratory, 3-1-5 Matsuyama, Kiyose City, Tokyo 204-0022, Japan. seki@bcg.gr.jp
authors.forename: Masaaki
authors.initials: M
authors.lastname: Seki
authors.forename: Akimasa
authors.initials: A
authors.lastname: Sato
authors.forename: Ikuro
authors.initials: I
authors.lastname: Honda
authors.forename: Toshio
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Ikuya
authors.initials: I
authors.lastname: Yano
authors.forename: Akira
authors.initials: A
authors.lastname: Koyama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Toida
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D001500:BCG Vaccine / Q000008:administration & dosage / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D007167:Immunotherapy; D007267:Injections; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000235:genetics; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014041:Tokyo; D001743:Urinary Bladder; D001749:Urinary Bladder Neoplasms / Q000517:prevention & control
chemical_list: D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D004269:DNA, Bacterial