title: Antigenaemia and antibody response to Toxoplasma gondii in human immunodeficiency virus-infected patients.
abstract: Toxoplasma encephalitis in immunocompromised patients results from reactivation of previously acquired (latent) infection. The aim of the study is to assess the antigenaemia and antibody response to Toxoplasma gondii in human immunodeficiency virus (HIV)-infected patients to determine the best marker for early diagnosis of toxoplasmosis in such patients. Indirect enzyme-linked immunosorbent assay (ELISA) for detection of IgG, IgM and IgA anti-toxoplasma antibodies and double-sandwich ELISA for toxoplasma antigen is carried out in serum samples collected from 100 HIV seropositive patients and 75 controls. Toxoplasma-specific IgG, IgM and IgA antibody response and antigenaemia were detected in 12%, 6%, 7% and 14% of HIV-infected patients, respectively. On retrospective analysis of 14 patients with antigenaemia only one had central nervous system (CNS) symptoms attributable to toxoplasma infection. In this patient, the CD4+ cell count was below 50/microL and none of the specific immunoglobulin isotype responses could be detected. The patient showed clinical improvement following specific chemotherapy for toxoplasmosis. In 25 HIV-negative and anti-toxoplasma IgG antibody-positive controls, IgM was detected in two (8%), IgA in five (20%) and antigenaemia in 10 (40%), while 50 HIV seronegative healthy controls were negative for both antigen and antibody responses. The study indicates that detection of toxoplasma antigen in addition to IgG antibody response may prove to be a useful indicator in the early diagnosis of reactivated toxoplasmosis in HIV/AIDS patients.
authors.affiliation: Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India. medinst@pgi.chd.nic.in
authors.forename: N
authors.initials: N
authors.lastname: Malla
authors.forename: C
authors.initials: C
authors.lastname: Sengupta
authors.forename: M L
authors.initials: ML
authors.lastname: Dubey
authors.forename: A
authors.initials: A
authors.lastname: Sud
authors.forename: U
authors.initials: U
authors.lastname: Dutta
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000097:blood*; D018791:CD4 Lymphocyte Count; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D012189:Retrospective Studies; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M