Magnetomechanical detection of the specific activities of endonucleases by cantilevers.
Thiolated nucleic acids 1 or 2 are immobilized on Au-coated cantilevers and hybridized with the complementary nucleic acids 1a or 2a associated with magnetic particles. The duplexes 1/1a or 2/2a include specific sequences for the scission by Apa I or Mse I, respectively. The cantilevers positioned in a flow cell are subjected to an external magnetic field, leading to the deflection of the cantilevers. Upon the specific scission of the DNA duplexes by Apa I or Mse I, the magnetic particles are disconnected from the cantilevers leading to their retraction to the rest position. The deflection/retraction of the cantilevers are followed by a conventional atomic force microscope optical detection system.
Weizmann
Yossi
Y
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Elnathan
Roey
R
Lioubashevski
Oleg
O
Willner
Itamar
I
D004247:DNA / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008280:Magnetics; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*
D004247:DNA; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases