Examination of the Lunatic fringe and Uncx4.1 expression by whole-mount in situ hybridization in the embryo of the CKH-Jsr (jumbled spine and ribs) mouse.
The CKH-Jsr (jumbled spine and ribs) mouse was found as a spontaneous mutant with malformation of vertebrae, that is, a short trunk and kinky tail. We examined Lunatic Fringe (Lfng) and Uncx4.1 expression in the presomitic mesoderm (PSM) and somites of Jsr-mutant (CKH-Jsr/+) embryos to elucidate pathogenesis of the Jsr mutation. Expression pattern of Lfng in the PSM of Jsr-mutant embryos was similar to that of the normal (C57BL/6) embryos. However, expression pattern of Uncx4.1 in the somites of Jsr-mutant embryos was impaired to be irregular and mosaic, suggesting that the anterior-posterior (A-P) polarity is disordered in the Jsr mutant. These results indicate that the Jsr mutation disrupts the A-P polarity of somites during the somitogenesis without altering Lfng expression pattern in the PSM.
Okano
Shinya
S
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0818.
Asano
Atsushi
A
Sasaki
Nobuya
N
Kon
Yasuhiro
Y
Watanabe
Tomomasa
T
Agui
Takashi
T
D000818:Animals; D019521:Body Patterning; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D016695:Glycosyltransferases / Q000096:biosynthesis*; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D008817:Mice, Mutant Strains / Q000196:embryology*; D009154:Mutation; D019170:Somites / Q000378:metabolism
D018398:Homeodomain Proteins; C108545:Uncx4.1 protein, mouse; D016695:Glycosyltransferases; C498392:Lfng protein, mouse