Kinetic study of the degradation of a potential local anesthetic drug in serum using the DNA-based electrochemical biosensor.
DNA-drug association interaction at the DNA modified screen-printed electrode for 1-methyl-2-piperidinoethylester of 2-hexoxyphenylcarbamic acid was found leading to an accumulation of the drug within the DNA layer. A procedure for the determination of drug in blood serum matrix using the protein precipitation and voltammetric measurement of the electroactive drug with the DNA biosensor was obtained and an effort was done to apply it for an assay of the drug enzymatic degradation in human and rabbit sera at 37 degrees C.
Vanícková
M
M
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-81237 Bratislava, Slovak Republic.
Lehotay
J
J
Cizmárik
J
J
Labuda
J
J
D000779:Anesthetics, Local / Q000097:blood* / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D018736:DNA Adducts / Q000097:blood*; D006801:Humans; D007700:Kinetics; D048448:Phenylcarbamates / Q000097:blood* / Q000378:metabolism*; D010880:Piperidines / Q000097:blood* / Q000378:metabolism*; D011817:Rabbits
C503086:2-hexoxyphenylcarbamic acid 1-methyl-2-piperidinoethylester; D000779:Anesthetics, Local; D018736:DNA Adducts; D048448:Phenylcarbamates; D010880:Piperidines; D002265:Carboxylic Ester Hydrolases; C077242:carbamate hydrolase