Array comparative genomic hybridization identifies genetic subgroups in grade 4 human astrocytoma.
Alterations of DNA copy number are believed to be important indicators of tumor progression in human astrocytoma. We used an array of bacterial artificial chromosomes to map relative DNA copy number in 50 primary glioblastoma multiforme tumors at approximately 1.4-Mb resolution. We identified 33 candidate sites for amplification and homozygous deletion in these tumors. We identified three major genetic subgroups within these glioblastoma multiforme tumors: tumors with chromosome 7 gain and chromosome 10 loss, tumors with only chromosome 10 loss in the absence of chromosome 7 gain, and tumors without copy number change in chromosomes 7 or 10. The significance of these genetic groups to therapeutics needs further study.
Misra
Anjan
A
Brain Tumor Research Center, Department of Neurosurgery, University of California San Francisco, San Francisco, California, USA. amisra@cc.ucsf.edu
Pellarin
Malgorzata
M
Nigro
Janice
J
Smirnov
Ivan
I
Moore
Dan
D
Lamborn
Kathleen R
KR
Pinkel
Daniel
D
Albertson
Donna G
DG
Feuerstein
Burt G
BG
D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D016000:Cluster Analysis; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015203:Reproducibility of Results; D016019:Survival Analysis