title: Influence of methyl jasmonate on podophyllotoxin and 6-methoxypodophyllotoxin accumulation in Linum album cell suspension cultures.
abstract: The accumulation of podophyllotoxin (PTOX) and 6-methoxypodophyllotoxin (6MPTOX) was enhanced about twofold in the suspension culture of Linum album line 2-5 aH following the addition of methyl jasmonate (MeJas) to the cultivation medium, reaching 7.69+/-1.45 mg/g dry weight and 1.11+/-0.09 mg/g dry weight, respectively. There was no increase in 6MPTOX accumulation following the addition of MeJas to suspension cells of L. album line X4SF, whereas PTOX accumulation was enhanced about tenfold to 0.49+/-0.10 mg/g dry weight. Phenylalanine ammonia-lyase activity increased immediately after the addition of MeJas to a cell suspension culture of line X4SF, reaching a maximum between 4 h and 1 day after elicitation, while cinnamyl alcohol dehydrogenase activity and the lignin content of the cells were not affected.
authors.affiliation: Heinrich-Heine-Universität Düsseldorf, Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Universitätsstr.1, 40225, Düsseldorf, Germany.
authors.forename: Betina
authors.initials: B
authors.lastname: van Fürden
authors.forename: Anne
authors.initials: A
authors.lastname: Humburg
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Fuss
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D019597:Flax / Q000187:drug effects / Q000378:metabolism*; D017705:Lignans / Q000096:biosynthesis*; D054883:Oxylipins; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis; D011034:Podophyllotoxin / Q000096:biosynthesis* / Q000031:analogs & derivatives
chemical_list: C502514:6-methoxypodophyllotoxin; D000085:Acetates; D003517:Cyclopentanes; D017705:Lignans; D054883:Oxylipins; C072239:methyl jasmonate; D000429:Alcohol Oxidoreductases; C018656:cinnamyl alcohol dehydrogenase; D010650:Phenylalanine Ammonia-Lyase; D011034:Podophyllotoxin