Antioxidant and antigenotoxic effects of plant cell wall hydroxycinnamic acids in cultured HT-29 cells.
We demonstrate that two hydroxycinnamic acids, (E )-ferulic acid and (E )-p-coumaric acid, have the ability to protect against oxidative stress and genotoxicity in cultured mammalian cells. They also show the ability to reduce the activity of the xenobiotic metabolising enzyme, cytochrome P450 1A, and downregulate the expression of the cyclooxygenase-2 enzyme. At equitoxic doses, their activities are equal to or superior to that of the known anticarcinogen, curcumin. The hydroxycinnamic acids are both important components of plant cell walls in certain plant foods. It is known that the action of microbial hydroxycinnamoyl esterases can lead to the release of hydroxycinnamic acids from ester-linkages to cell wall polysaccharides into the human colon. Thus, providing they can reach effective levels in the colon, they could provide an important mechanism by which dietary fibres of food plants, such as spinach or cereal, protect against colon cancer.
Ferguson
Lynnette R
LR
Discipline of Nutrition, Faculty of Medicine and Health Sciences. The University of Auckland, Auckland, New Zealand. l.ferguson@auckland.ac.nz
Zhu
Shuo-tun
ST
Harris
Philip J
PJ
D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry*; D003373:Coumaric Acids / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology; D051546:Cyclooxygenase 2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004043:Dietary Fiber; D005982:Glutathione Transferase / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D008565:Membrane Proteins; D010945:Plants, Edible / Q000648:ultrastructure*; D011422:Propionates; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
D016587:Antimutagenic Agents; D000975:Antioxidants; D003373:Coumaric Acids; D004043:Dietary Fiber; D008565:Membrane Proteins; D011422:Propionates; D003577:Cytochrome P-450 Enzyme System; C004999:ferulic acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D005982:Glutathione Transferase; C495469:p-coumaric acid; D003474:Curcumin