Detoxification of corn stover and corn starch pyrolysis liquors by ligninolytic enzymes of Phanerochaete chrysosporium.
Phanerochaete chrysosporium (ATCC 24725) shake flask culture with 3 mM veratryl alcohol addition on day 3 was able to grow and detoxify different concentrations of diluted corn stover (Dcs) and diluted corn starch (Dst) pyrolysis liquors [10, 25, and 50% (v/v)] in defined media. GC-MS analysis of reaction products showed a decrease and change in some compounds. In addition, the total phenolic assay with Dcs samples demonstrated a decrease in the phenolic compounds. A bioassay employing Lactobacillus casei growth and lactic acid production was developed to confirm the removal of toxic compounds from 10 and 25% (v/v) Dcs and Dst by the lignolytic enzymes, but not from 50% (v/v) Dcs and Dst. The removal did not occur when sodium azide or cycloheximide was added to Ph. chrysosporium culture media, confirming the participation of lignolytic enzymes in the detoxification process. A concentrated enzyme preparation decreased the phenolic compounds in 10% (v/v) corn stover and corn starch pyrolysis liquors to the same extent as the fungal cultures.
Khiyami
Mohammad A
MA
Department of food Science and Human Nutrition and Center for Sustainable Environmental Technology, Iowa State University, Ames, Iowa 50010, USA.
Pometto
Anthony L
AL
Brown
Robert C
RC
D003513:Cycloheximide / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D008658:Inactivation, Metabolic; D019344:Lactic Acid / Q000096:biosynthesis; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D010636:Phenols / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology; D013213:Starch / Q000737:chemistry / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
D010636:Phenols; D019344:Lactic Acid; D013213:Starch; D008031:Lignin; D019810:Sodium Azide; D003513:Cycloheximide