Global real-time quantification/reverse transcription-polymerase chain reaction for detecting proto-oncogenes associated with 14q32 chromosomal translocation in multiple myeloma.
A global real-time quantitative/reverse transcription-polymerase chain reaction technique for detecting the expression of six 14q32 chromosomal translocation-associated proto-oncogenes in marrow plasma cells was established and applied to myeloma specimens. This technique is an alternative method of detecting 14q32 rearrangements and allows investigation of the relationship between proto-oncogene expression and clinical features.
Tajima
Emi
E
Uranishi
Miyuki
M
Iida
Shinsuke
S
Komatsu
Hirokazu
H
Nitta
Masakazu
M
Ueda
Ryuzo
R
D002883:Chromosomes, Human, Pair 14; D015870:Gene Expression; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014178:Translocation, Genetic / Q000235:genetics*