title: Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.
abstract: Transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP) are useful as marker lines in chimera experiments. We established a new embryonic stem (ES) cell line (named B6G-2) from a C57BL/6 blastocyst showing ubiquitous EGFP expression. Undifferentiated B6G-2 cells showed strong green fluorescence and mRNAs of pluripotent marker genes. B6G-2 cells were transferred into a C57BL/6 blastocyst to generate a germline chimera, the progeny of which inherited ubiquitous EGFP expression. Mice derived completely from B6G-2 cells were also developed from the ES cells; these were tetraploid chimeras. The established B6G-2 cells were shown to be pluripotent and to be capable of differentiating into cells of all lineages. Thus, the new ES cell line expressing EGFP ubiquitously is useful for basic research in the field of regenerative medicine. The B6G-2 cell line is freely available from the BioResource Center, RIKEN Tsukuba Institute (http://www.brc.riken.jp/lab/cell/english/).
authors.affiliation: Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Shimizukawa
authors.forename: Aya
authors.initials: A
authors.lastname: Sakata
authors.forename: Michiko
authors.initials: M
authors.lastname: Hirose
authors.forename: Akio
authors.initials: A
authors.lastname: Takahashi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Iseki
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kunita
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Sugiyama
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yagami
mesh_terms: D000818:Animals; D001755:Blastocyst; D002454:Cell Differentiation; D002678:Chimera; D005260:Female; D005819:Genetic Markers; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011003:Ploidies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells; D014018:Tissue Distribution
chemical_list: D005819:Genetic Markers; D049452:Green Fluorescent Proteins