A reproducible model of cardiac hypertrophy in rats. Problems and obstacles we faced.
OBJECTIVE
To develop a reproducible model of significant left ventricular hypertrophy in order to study the role played by the tumor suppressor protein 53 (P53) in the mechanisms of cardiac hypertrophy, the cross-talk with the other factors and the connection between expression and activity of P53, cardiac myocyte apoptosis and heart hypertrophy; to discuss the problems and obstacles we faced.


METHODS
Male Wistar rats were used in the experiments. Left ventricular hypertrophy was induced by banding the abdominal aorta. Three series of the experiment were performed differing in the place of banding and the size of constriction in order to find the most suitable model for our further research.


RESULTS
Forty five days after banding the abdominal aorta just below the diaphragm the cardiac index increased by 30% in comparison with that in the controls.


CONCLUSIONS
We have developed a reproducible model of significant left ventricular hypertrophy in rats. The main advantages of the model are: a) it is technically simple; b) it may be realised in any laboratory all over the world; c) it allows the most common lab animals (male Wistar rats) to be used for studies without having to be subjected to thoracotomy and hence requiring a long recovery period.
Stoyanova
Vili K
VK
Department of Pediatrics and Medical Genetics, Medical University, Plovdiv, Bulgaria. vilsto@yahoo.com
Ghenev
Evghenii D
ED
Yanev
Ivan B
IB
Yanev
Tony K
TK
Nachev
Chudomir K
CK
D000818:Animals; D004195:Disease Models, Animal; D017379:Hypertrophy, Left Ventricular; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results