title: [Determination of protein in urine by spectral probe of molybdenum(VI) salicylfluoronate complex].
abstract: The absorption spectral behavior of binding reaction of molybdenum(VI)-salicylfluoronate on bovine serum albumin was investigated. A novel method for the determination of protein in urine using Mo(VI)-salicylfluoronate complex as a spectroprobe was developed. The maximum binding number (n) of molybdenum complex to BSA was found to be 100. The molar absorptivity of binding product at 575 nm was 2.86 x 10(6) L x mol(-1) x cm(-1). The method possesses high sensitivity as well as high selectivity. It could be used to determine protein in human urine directly. The relative standard deviation for the determination is 1.5%.
authors.affiliation: Department of Chemistry, Nankai University, 300071 Tianjin.
authors.forename: H
authors.initials: H
authors.lastname: Shen
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
mesh_terms: D006801:Humans; D008982:Molybdenum / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D014556:Urine / Q000737:chemistry*
chemical_list: D011506:Proteins; D008982:Molybdenum