Characterization of Bacillus subtilis clones surviving overproduction of Zeta, a pSM19035 plasmid-encoded toxin.
The postsegregational killing system of pSM19035 plasmid consists of the proteins Zeta and Epsilon, a toxin and an antidote, respectively. Zeta mutants were isolated with the use of Bacillus subtilis strain with the zeta gene under control of an inducible promoter integrated into the chromosome. Results of mutant analysis point to the amino terminal part of the Zeta protein as being responsible for the toxicity.
Nowakowska
Beata
B
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
Kern-Zdanowicz
Izabela
I
Zielenkiewicz
Urszula
U
Cegłowski
Piotr
P
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic
D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial