[Purification of chymopapain and its effect on nucleus pulposus in vitro].
OBJECTIVE
To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.


METHODS
Chymopapain was purified by ion exchange chromatograph and tested its effect on nucleus pulposus in vitro.


RESULTS
A simple and effective procedure for purification of chymopapain was established and the chymopapain did degrade nucleus pulposus.


CONCLUSIONS
The ion exchange chromatograph was a simple and effective procedure for purification of chymopapain. It is necessary to study its effect on nucleus pulposus in vivo.
Deng
Jing
J
General Hospital of Guangzhou Military Region, Guangzhou.
Wang
Jianhua
J
Luo
Xingen
X
Zhao
Shujin
S
D031042:Asimina / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002917:Chymopapain / Q000302:isolation & purification / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000187:drug effects; D007404:Intervertebral Disc Chemolysis; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D003094:Collagen; D002917:Chymopapain