title: Preliminary evaluation of a unique freezing technology for bovine spermatozoa cryopreservation.
abstract: Artificial insemination (AI) and semen cryopreservation has significantly improved the breeding potential of male animals. However, current freezing techniques commonly result in reduced semen quality. Ten years ago, a unique freezing technology (UFT) was developed for the freezing of foodstuffs and other materials. Previous work from this laboratory has demonstrated the UFT to be a superior method of freezing for a number of cell types. In a preliminary study, the UFT was compared with the conventional freezing methodology of bovine semen. Semen samples were collected from an angus (Bull A) and a gelbivich bull (Bull B), prepared using a conventional bovine cryoprotectant, and frozen in the UFT or in liquid nitrogen (LN) mist. The samples were stored in LN before being thawed and assessed for the semen parameters of motility and forward progression. Preliminary results suggest the UFT is equivalent to current techniques in the cryopreservation and recovery of bovine semen, and with modification, possibly a superior technique for semen freezing. Further studies using larger sample populations, and using a CASA system to evaluate motility, forward progression and linearity are merited.
authors.affiliation: Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79430, USA.
authors.forename: P Z
authors.initials: PZ
authors.lastname: Cotter
authors.forename: H A
authors.initials: HA
authors.lastname: Goolsby
authors.forename: S D
authors.initials: SD
authors.lastname: Prien
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013696:Temperature; D013997:Time Factors