Analysis of cerebellum proteomics in the hydrocephalic H-Tx rat.
The H-Tx rat is a polygenic inherited model of hydrocephalus. In order to identify disease-specific biomarkers associated with congenital hydrocephalus, comparative proteomic analysis was used to screen cerebellum proteins in H-Tx rats at 1 day after birth. Seven proteins showed significant changes in hydrocephalic H-Tx rats compared with Sprague-Dawley and normal H-Tx rats, including HMG-1, CDCrel-1A, mitochondrial ATP synthase, ERp29, NADP+-ICDH, CCT beta and gamma. This indicates that the hydro-cephalus in H-Tx rats may be the result of a panel of proteins. In particular, the presence of HMG-1 and lack of CDCrel-1 in hydrocephalic H-Tx rats suggests that the use of two protein markers will provide further insight with respect to congenital hydrocephalus.
Li
Xianfeng
X
Department of Neurosurgery, Juntendo University School of Medicine, Tokyo, Japan. lix72@hotmail.com
Miyajima
Masakazu
M
Mineki
Reiko
R
Taka
Hikari
H
Murayama
Kimie
K
Arai
Hajime
H
D000818:Animals; D015415:Biomarkers; D002531:Cerebellum / Q000737:chemistry* / Q000503:physiopathology; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006849:Hydrocephalus / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology*; D010641:Phenotype; D040901:Proteomics; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley
D015415:Biomarkers