The KIF3 motor transports N-cadherin and organizes the developing neuroepithelium.
In the developing brain, the organization of the neuroepithelium is maintained by a critical balance between proliferation and cell-cell adhesion of neural progenitor cells. The molecular mechanisms that underlie this are still largely unknown. Here, through analysis of a conditional knockout mouse for the Kap3 gene, we show that post-Golgi transport of N-cadherin by the KIF3 molecular motor complex is crucial for maintaining this balance. N-cadherin and beta-catenin associate with the KIF3 complex by co-immunoprecipitation, and colocalize with KIF3 in cells. Furthermore, in KAP3-deficient cells, the subcellular localization of N-cadherin was disrupted. Taken together, these results suggest a potential tumour-suppressing activity for this molecular motor.
Teng
Junlin
J
Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Rai
Tatemitsu
T
Tanaka
Yosuke
Y
Takei
Yosuke
Y
Nakata
Takao
T
Hirasawa
Motoyuki
M
Kulkarni
Ashok B
AB
Hirokawa
Nobutaka
N
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000196:embryology / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D051379:Mice; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology; D046569:Neuroepithelial Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D015534:Trans-Activators / Q000378:metabolism; D051176:beta Catenin
D048868:Adaptor Proteins, Signal Transducing; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D020409:Molecular Motor Proteins; D015534:Trans-Activators; D051176:beta Catenin; C100815:Kifap3 protein, mouse