Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling.
Fructose-bisphosphate aldolase is a glycolytic enzyme whose activity increases in rice roots treated with gibberellin (GA). To investigate the relationship between aldolase and root growth, GA-induced root aldolase was characterized. GA3 promoted an increase in aldolase accumulation when 0.1 microM GA3 was added exogenously to rice roots. Aldolase accumulated abundantly in roots, especially in the apical region. To examine the effect of aldolase function on root growth, transgenic rice plants expressing antisense aldolase were constructed. Root growth of aldolase-antisense transgenic rice was repressed compared with that of the vector control transgenic rice. Although aldolase activity increased by 25% in vector control rice roots treated with 0.1 microM GA3, FBPA activity increased very little by 0.1 microM GA3 treatment in the root of aldolase-antisense transgenic rice. Furthermore, aldolase co-immunoprecipitated with antibodies against vacuolar H+ -ATPase in rice roots. In the root of OsCDPK13-antisense transgenic rice, aldolase did not accumulate even after treatment with GA3. These results suggest that the activation of glycolytic pathway function accelerates root growth and that GA3-induced root aldolase may be modulated through OsCDPK13. Aldolase physically associates with vacuolar H-ATPase in roots and may regulate the vacuolar H-ATPase mediated control of cell elongation that determines root length.
Konishi
Hirosato
H
Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan.
Yamane
Hisakazu
H
Maeshima
Masayoshi
M
Komatsu
Setsuko
S
D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D016373:DNA, Antisense / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology*; D047468:Immunoprecipitation; D007476:Ionophores / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D000001:Calcimycin; D002118:Calcium; D016373:DNA, Antisense; D005634:Fructose-Bisphosphate Aldolase; D005875:Gibberellins; D007476:Ionophores; D007811:Lanthanum; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011494:Protein Kinases; D012333:RNA, Messenger; D025262:Vacuolar Proton-Translocating ATPases; C028521:lanthanum chloride; C007842:gibberellic acid; C454865:calcium-dependent protein kinase, rice; D017871:Calcium-Calmodulin-Dependent Protein Kinases