title: Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.
abstract: OBJECTIVE
  To report the first birth after transfer of cryopreserved embryos generated by intracytoplasmic sperm injection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Tertiary center for reproductive technology.
  
  
  METHODS
  A 36-year-old woman with primary infertility of 3 years' duration and a 37-year-old man with congenital bilateral absence of the vas deferens.
  
  
  METHODS
  Cryopreservation of human embryos after oocytes and sperm thawing.
  
  
  METHODS
  Live birth.
  
  
  RESULTS
  A healthy, normal female infant with a birth weight of 2,950 g was born by cesarean section at 38 weeks' gestation, with normal 6-month follow-up.
  
  
  CONCLUSIONS
  Embryo cryopreservation can lead to successful results, even with the use of cryopreserved gametes.
authors.affiliation: Operative Unit Reproductive Medicine, Istituto Clinico Humanitas, Rozzano (Milan), Italy. paolo.levi_setti@humanitas.it
authors.forename: Paolo Emanuele
authors.initials: PE
authors.lastname: Levi Setti
authors.forename: Elena
authors.initials: E
authors.lastname: Albani
authors.forename: Paola Vittoria
authors.initials: PV
authors.lastname: Novara
authors.forename: Amalia
authors.initials: A
authors.lastname: Cesana
authors.forename: Silvia
authors.initials: S
authors.lastname: Bianchi
authors.forename: Luciano
authors.initials: L
authors.lastname: Negri
mesh_terms: D000328:Adult; D001724:Birth Weight; D002585:Cesarean Section; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005500:Follow-Up Studies; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology / Q000637:transplantation*; D014649:Vas Deferens / Q000002:abnormalities / Q000502:physiology