Monitoring processed, mature Human Immunodeficiency Virus type 1 particles immediately following treatment with a protease inhibitor-containing treatment regimen.
Protease inhibitors (PIs) block HIV-1 maturation into an infectious virus particle by inhibiting the protease processing of gag and gag-pol precursor proteins. We have used a simple anti-HIV-1 p24 Western blot to monitor the processing of p55gag precursor into the mature p24 capsid immediately following the first dosage of a PI-containing treatment regimen. Evidence of PI activity was observed in plasma virus as early as 72 hours post treatment-initiation and was predictive of plasma viral RNA decrease at 4 weeks.
Baird
Heather A
HA
Division of Infectious Disease, Department of Medicine, Case School of Medicine and the Center for AIDS Research, Case Western Reserve University, University Hospitals of Cleveland, Cleveland, OH, USA. eja3@case.edu.
Marozsan
Andre J
AJ
Lederman
Michael M
MM
Landay
Alan
A
Mildvan
Donna
D
Kuritzkes
Daniel R
DR
Kessler
Harold A
HA
Arts
Eric J
EJ