[Room temperature phosphorimetric determination of practolol with dansyl chloride labeling].
Dansyl Chloride (DNS-Cl) was used as a phosphorescent labeling agent for the determination of practolol. The derivative was formed by refluxing practolol-ethyl acetate solution with the solid DNS-Cl in the presence of potassium carbonate anhydride. Room-temperature phosphorescent (RTP) properties of the derivative were studied by a micelle-stabilized room-temperature phosphorescence (MS-RTP) method. Phosphorescence lifetimes of practolol-DNS and the DNS-Cl were found to be 0.791 and 0.408ms, respectively. By applying the derivative MS-RTP technique, practolol could be quantitatively determined without previous separation process. Detection limit of practolol with the proposed method was 0.19 microg/mL.
Tong
A
A
Department of Chemistry, Tsinghua University, 100084 Beijing.
Wu
Y
Y
Li
L
L
D003619:Dansyl Compounds / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D011217:Practolol / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry; D013696:Temperature
D003619:Dansyl Compounds; D013447:Sulfites; D012967:Sodium Dodecyl Sulfate; C029038:dansyl chloride; D011217:Practolol; C025026:sodium sulfite