Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2.
The accumulation of H2O2 by NaCl was observed in the roots of rice seedlings. Treatment with NaCl caused an increase in the activities of ascorbate peroxidase (APX) and glutathione reductase (GR) and the expression of OsAPX and OsGR in rice roots. Exogenously applied H2O2 also enhanced the activities of APX and GR and the expression of OsAPX and OsGR in rice roots. The accumulation of H2O2 in rice roots in response to NaCl was inhibited by the NADPH oxidase inhibitors, diphenyleneiodonium chloride (DPI) and imidazole (IMD). However, DPI, IMD, and dimethylthiourea, a H2O2 trap, did not reduce NaCl-enhanced activities of APX and GR and expression of OsAPX and OsGR. It appears that H2O2 is not involved in the regulation of NaCl-induced APX and GR activities and OsAPX and OsGR expression in rice roots.
Tsai
Yu-Chang
YC
Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.
Hong
Chwan-Yang
CY
Liu
Li-Fei
LF
Kao
Ching Huei
CH
D060387:Ascorbate Peroxidases; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005980:Glutathione Reductase / Q000187:drug effects / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D012275:Oryza / Q000201:enzymology; D010544:Peroxidases / Q000187:drug effects / Q000235:genetics*; D036226:Seedlings / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology*
D018076:DNA, Complementary; D018744:DNA, Plant; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D005980:Glutathione Reductase