title: [The effect of envelope protein mutation on hepatitis B virus assemble].
abstract: OBJECTIVE
  To investigate the effect of envelope protein mutation on HBV assembly.
  
  
  METHODS
  The envelope protein mutated vectors were constructed by the molecular clone in vitro, and then transfected transiently in the cell HepG2. The expression and secretion of S protein was assay by ELISA. HBV DNA was quantitatively evaluated by PCR. After co-transfection with pHBV-mS1S and adwR9 the DNA was quantitatively evaluated by PCR.
  
  
  RESULTS
  There was no significant difference in expression and secretion of S protein assayed by ELISA in the cytoplasm and supernatant among pHBV-mS1, pHBV-mS, pHBV-mS1S and the wild HBV adwR9 plasmid. The DNA detected by real-time fluorescence quantitative PCR from those the cytoplasm of mutants was higher than that from the wild HBV adwR9 cytoplasm, especially from the cytoplasm of pHBV-mS1S plasmid. However, the DNA detected by real-time fluorescence quantitative PCR from the supernatant of those mutants was lower than that from the wild HBV adwR9 supernatant, especially from pHBV-mS1S. The DNA detected by real-time fluorescence quantitative PCR from the supernatant co-transfected with pHBV-mS1S and adwR9 was lower than that from the supernatant co-transfected with pcDNA3 and adwR9.
  
  
  CONCLUSIONS
  There was no effect of envelope protein mutation on the expression and secretion of S protein. Envelope protein mutation could interfere the assembly of HBV particle and cause reduction of secretion of HBV.
authors.affiliation: Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Di
authors.forename: Da-rong
authors.initials: DR
authors.lastname: Hu
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Xiong
authors.forename: Xue-ling
authors.initials: XL
authors.lastname: Hu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Gong-ren
authors.initials: GR
authors.lastname: Fan
authors.forename: Yi-pin
authors.initials: YP
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*; D019065:Virus Assembly
chemical_list: D006514:Hepatitis B Surface Antigens; D014759:Viral Envelope Proteins