[Study on the effect of composite of basic fibroblast growth factor and partially deproteinized bone on the repair of femoral head defects].
OBJECTIVE
To evaluate the effect of composite (bFGF/PDPB) of basic fibroblast growth factor (bFGF) and partially deproteinized bone (PDPB) on the repair of femoral head defect.


METHODS
Forty-eight femoral heads with defect derived from 24 New Zealand rabbits were divided into 3 groups at random, which were implanted with bFGF/PDPB (group A), PDPB (group B) and nothing (group C) respectively. The rabbits were sacrificed at 2, 4, and 8 weeks after operation, and then the femoral heads were obtained. The specimens injected with Chinese ink were created. Then X-ray examination, histopathological and morphological examination of blood vessel, and image analysis were made.


RESULTS
The bone defects healed completely 8 weeks after operation in group A. The implants in the repaired tissue were not substituted completely in group B. The bone defects did not heal completely in group C. Two weeks after operation, affluent newly formed vessels were seen in repaired areas in group A. No significant difference between group A and group B was observed 8 weeks after operation. In group C, newly formed vessels were scarce 2, 4, and 8 weeks after operation. There were 3 sides rated excellent, 2 good and 1 fair in group A; 1 excellent, 2 good, 2 fair and 1 poor in group B; and 1 fair and 5 poor in group C according to the X-ray evaluation 8 weeks after operation. Eight weeks after operation, the volume fraction of bone trabecula in repaired tissue was higher in group A than that in group B (P < 0.05), and the fraction in group C was the lowest among the 3 groups (P < 0.05).


CONCLUSIONS
The composite of bFGF and PDPB can effectively promote the repair of femoral head defect of rabbit.
Li
Xiaohui
X
Department of Orthopedics, West China Hospital of Sichuan University, Sichuan Unversity, Chengdu Sichuan 610041, PR China.
Gong
Yuekun
Y
Song
Yueming
Y
Li
Shihe
S
Li
Yanlin
Y
Yang
Hao
H
Xu
Xiaohui
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D005270:Femur Head / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D005271:Femur Head Necrosis / Q000601:surgery*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation
D001672:Biocompatible Materials; D018786:Bone Substitutes; D016222:Fibroblast Growth Factor 2