title: [Bioactive effectiveness of selected disinfective agents on Gram-negative bacilli isolated from hospital environment].
abstract: In our study the susceptibility (MIC) of chosen 21 strains of Gram-negative bacilli isolated in hospitals to disinfectant agents (glucoprotamine, sodium dichloroisocyanurate, potassium persulfate), the effectiveness of these disinfectants against selected bacteria and their effectiveness to biofilm forming bacteria was determined. It was found that glucoprotamine showed the highest activity to Gram-negative bacteria. Obtained MIC values for glucoprotamine (except 1 strain of S. marcescens) were 16-64 times lower that MICs for sodium dichloroisocyanurate and 4-32 times lower that MICs for potassium persulfate. The effectiveness of disinfectants containing potassium persulfate or sodium dichloroisocyanurate was 100% tested by carrier method. Glucoprotamine was ineffective against 2 out of 9 strains (18%): E. cloacae and S. marcescens. It was found that disinfectants were more effective against Gram-negative bacteria in carrier methods than for biofilm forming bacteria. 86% of bacteria growing 5 days on a catheter were resistant to working solution of disinfectant containing glucoprotamine (5200 mg/L) or potassium persulfate (4300 mg/L); 66.6% of tested bacteria were resistant to working solution of sodium dichloroisocyanurate (1795.2 mg/L). In our study the highest effectiveness to biofilm forming bacteria showed disinfectant with sodium dichloroisocyanurate, the lowest--with glucoprotamine.
authors.affiliation: Zakład Bakteriologii, Państwowego Zakładu Higieny, Warszawa. kpancer@pzh.gov.pl
authors.forename: Katarzyna W
authors.initials: KW
authors.lastname: Pancer
authors.forename: Agnieszka E
authors.initials: AE
authors.lastname: Laudy
authors.forename: Ewa
authors.initials: E
authors.lastname: Mikulak
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Gliniewicz
authors.forename: Monika
authors.initials: M
authors.lastname: Staniszewska
authors.forename: Hanna
authors.initials: H
authors.lastname: Stypułkowska-Misiurewicz
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D003058:Cockroaches; D003902:Detergents / Q000494:pharmacology; D003959:Diamines / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000592:standards*; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control*; D006797:Housekeeping, Hospital / Q000592:standards*; D006801:Humans; D008284:Maintenance and Engineering, Hospital / Q000592:standards; D011044:Poland; D017680:Potassium Compounds / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology
chemical_list: D003902:Detergents; D003959:Diamines; D004202:Disinfectants; D017680:Potassium Compounds; D011760:Pyrrolidinones; D013431:Sulfates; D014227:Triazines; C089578:glucoprotamine; C009007:potassium persulfate; C011765:troclosene