Enhanced radical scavenging activity of genetically modified Arabidopsis seeds.
The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1 cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.
Tohge
Takayuki
T
Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan.
Matsui
Kyoko
K
Ohme-Takagi
Masaru
M
Yamazaki
Mami
M
Saito
Kazuki
K
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D001709:Biotechnology; D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000378:metabolism; D005609:Free Radicals; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005818:Genetic Engineering / Q000379:methods*; D008956:Models, Chemical; D009154:Mutation; D000073718:Pancreatitis-Associated Proteins; D030821:Plants, Genetically Modified; D044945:Proanthocyanidins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012639:Seeds; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D005419:Flavonoids; D005609:Free Radicals; D000073718:Pancreatitis-Associated Proteins; D044945:Proanthocyanidins; D020598:Proto-Oncogene Proteins c-myb; C000612141:REG3A protein, human; D011993:Recombinant Fusion Proteins; C013221:proanthocyanidin