title: Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischaemia induced by percutaneous coronary intervention.
abstract: OBJECTIVE
  Cardiac markers such as troponin T (c-TnT) have proven unsuitable for the detection of early and transient myocardial ischaemia. We recently reported that abrupt elevation of serum deoxyribonuclease I (DNase I) activity in the early stage of acute myocardial infarction could be used as a diagnostic marker. To evaluate whether serum DNase I could be used as a marker of early myocardial ischaemia, we investigated alterations in its levels after transient ischaemia induced during percutaneous coronary intervention (PCI).
  
  
  RESULTS
  In 24 consecutive patients with stable angina undergoing elective PCI and 12 patients undergoing coronary angiography (CAG), serum samples were tested for DNase I, creatine kinase isoenzyme MB (CK-MB), and c-TnT before, soon after, and 3 and 12-24 h after completion of the procedures. Serum DNase I activity had risen significantly from baseline by 3 h after PCI in 21 of the 24 PCI patients. The mean per cent difference from baseline in serum DNase I activity 3 h after PCI was 35.9+/-37.5%. Even among the 16 PCI patients whose levels of CK-MB and c-TnT were within the normal range, 13 showed elevation of serum DNase I activity from baseline after PCI. In the CAG patient group, DNase I activity levels remained unchanged at all times after CAG.
  
  
  CONCLUSIONS
  Elevation of serum DNase I activity can be used as a sensitive marker for detection of transient myocardial ischaemia.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Arakawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kawai
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Norihiro
authors.initials: N
authors.lastname: Morikawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tada
authors.forename: Ren
authors.initials: R
authors.lastname: Kawaguchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kishi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003850:Deoxyribonuclease I / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood / Q000175:diagnosis* / Q000209:etiology
chemical_list: D015415:Biomarkers; D003850:Deoxyribonuclease I