[Suppression subtractive hybridization to identify differentially expressed gene between laryngeal tumor and the normal tissue adjacant to the tumor].
OBJECTIVE
To screen the differently expressed genes between laryngeal tumor and the normal tissue adjacant to the tumor and construct the subtractive cDNA library.


METHODS
The subtractive cDNA library was contructed with the suppression subtractive hybridization (SSH) technique. Nine random clones are made as probes for the reverse Northern hybridization, after sequenced. The sequences are compared with known sequences using blastn against GenBank across the Internet.


RESULTS
Except one clone ligated by two adaptor, the others represented six gene and one IgG heavy chain constant region. Among the six genes, one was a new kind of antibody whose function was not understood,the others stood for genes as follows: keratin, beta-actin, myosin, tumor necrosis factor, alkaline phosphatase.


CONCLUSIONS
Screening the differently expressed gene with SSH is reliable. These genes play a pivotal role in tumorigenesis, tumor promotion, tumor metastasis, tumor immunity and tumor marker.
Zhou
Wensheng
W
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Wang
Jibao
J
D000368:Aged; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*
D018076:DNA, Complementary