Levels of sulfite in three organs from mice exposed to sulfur [corrected] dioxide.
To study whether sulfur dioxide (SO(2)) can enter into the different organs of mice exposed to SO(2), the sulfite contents in brains, hearts, and lungs from male mice were determined by high-performance liquid chromatography with fluorescence detection (HPLC-FD). After reduction and precolumn derivation of tissue homogenates of brains, hearts, and lungs from mice, the mixture was centrifuged, and 5 microl of the resulting supernatant was directly injected into HPLC; the mobile phase consisted of methanol-phosphoric acid (12:88, v/v), and for the fluorescence detection lambdaEX 392 nm and lambdaEM--479 nm were used. The standard curve was linear in the range from 0.126 microg/ml to 126 microg/ml; the minimal detectable concentration was 0.04 microg/ml (S/N = 3), the average methodological recoveries were from 97% to 101%, and the within-day and between-day precisions were less than 9%. These results showed that sulfite contents in all organs tested from mice in the SO(2)-exposed groups were significantly increased (p < .05) in a dose-dependent manner (r > .92) compared with the control groups. These results indicated SO(2) could transform into sulfite in vivo after inhalation, and could distribute into lung and other organs such as brain and heart. These results offered a support for the viewpoint that SO(2) is a systemic toxic agent.
Meng
Ziqiang
Z
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan, China. zqmeng@sxu.edu.cn
Li
Ruijin
R
Zhang
Xin
X
D000280:Administration, Inhalation; D000825:Animal Structures / Q000737:chemistry* / Q000187:drug effects*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006321:Heart / Q000187:drug effects; D008168:Lung / Q000737:chemistry / Q000187:drug effects; D008297:Male; D051379:Mice; D009206:Myocardium / Q000737:chemistry; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013447:Sulfites / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D013458:Sulfur Dioxide / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D014018:Tissue Distribution
D013447:Sulfites; D013458:Sulfur Dioxide