title: The highly conserved tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex and is required for the G2/M phase transition in the yeast Saccharomyces cerevisiae.
abstract: Here we show that the Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p interacts with the origin recognition complex in vivo and in vitro and that overexpression of hemagglutinin-Thg1p selectively impedes growth of orc2-1(Ts) cells at the permissive temperature. Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast.
authors.affiliation: Department of Pathology, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont 05405, USA.
authors.forename: Terri S
authors.initials: TS
authors.lastname: Rice
authors.forename: Min
authors.initials: M
authors.lastname: Ding
authors.forename: David S
authors.initials: DS
authors.lastname: Pederson
authors.forename: Nicholas H
authors.initials: NH
authors.lastname: Heintz
mesh_terms: D002455:Cell Division; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016195:G2 Phase; D006388:Hemagglutinins / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D051738:Origin Recognition Complex; D012354:RNA, Transfer, His; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006388:Hemagglutinins; D051738:Origin Recognition Complex; D012354:RNA, Transfer, His; D029701:Saccharomyces cerevisiae Proteins; D009713:Nucleotidyltransferases; C020842:guanylyltransferase