Hepatoprotective effect of lactic acid bacteria, inhibitors of beta-glucuronidase production against intestinal microflora.
The hepatoprotective activity of lactic acid bacteria (Lactobacillus brevis HY7401, Lactobacillus acidophilus CSG and Bifidobacterium longum HY8001), which inhibited beta-glucuronidase productivity of intestinal microflora, on t-BHP- or CCl4-induced hepatotoxicity of mice were evaluated. These oral administration of lactic acid bacteria lowered beta-glucuronidase production of intestinal microflora as well as Escherichia coli HGU-3. When lactic acid bacteria at a dose of 0.5 or 2 g (wet weight)/kg was orally administered on CCl4-induced liver injury in mice, these bacteria significantly inhibited the increase of plasma alanine transferase and aspartate transferase activities by 17-57% and 57-66% of the CCl4 control group, respectively. These lactic acid bacteria also showed the potent hepatoprotective effect against t-BHP-induced liver injury in mice. The inhibitory effects of these lactic acid bacteria were more potent than that of dimethyl diphenyl bicarboxylate (DDB), which have been used as a commercial hepatoprotective agent. Among these lactic acid bacteria, L. acidophilus CSG exhibited the most potent hepatoprotective effect. Based on these findings, we insist that an inhibitor of beta-glucuronidase production in intestine, such as lactic acid bacteria, may be hepatoprotective.
Han
Song-Yi
SY
College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea.
Huh
Chul-Sung
CS
Ahn
Young-Tae
YT
Lim
Kwang-Sei
KS
Baek
Young-Jin
YJ
Kim
Dong-Hyun
DH
D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D002252:Carbon Tetrachloride Poisoning / Q000150:complications; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D018920:Coculture Techniques; D004926:Escherichia coli / Q000254:growth & development; D005966:Glucuronidase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D007422:Intestines / Q000201:enzymology / Q000382:microbiology*; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020122:tert-Butylhydroperoxide
D019344:Lactic Acid; D020122:tert-Butylhydroperoxide; D005966:Glucuronidase