[Association between defects of karyogamy and translation termination in yeast Saccharomyces cerevisiae].
Mutants capable of a high frequency of cytoduction (Hfc+) were obtained in a haploid strain of Saccharomyces cerevisiae, suggesting impaired cytogamy. Nine of the 68 Hfc+ mutants showed the antisuppressor effect with respect to mutations of the SUP35 and SUP45 genes, which code for translation termination factors, or to the [PSI+] factor, which is the prion form of Sup35. Cosegregation of the characters "higher frequency of cytoduction" and "antisuppression" was demonstrated for three Hfc+ mutants. One (HFC12-2) of the Hfc+ mutations exerted a dominant antisuppressor effect with respect to [PSI+] and had no effect on [PSI+] maintenance. On the strength of the results, an interaction was assumed for translation termination components and cytoskeleton proteins, which play a role in karyogamy in yeasts.
Borchsenius
A S
AS
Repnevskaia
M V
MV
Kurischko
C
C
Inge-Vechtomov
S G
SG
D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D006238:Haploidy; D009154:Mutation; D010454:Peptide Termination Factors / Q000235:genetics* / Q000378:metabolism; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic
D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins