Transgenic mice with pancellular enhanced green fluorescent protein expression in primitive hematopoietic cells and all blood cell progeny.
Transgenic mice homogeneously expressing enhanced green fluorescence protein (EGFP) in primitive hematopoietic cells and all blood cell progeny, including erythrocytes and platelets, have not been reported. Given previous data indicating H2Kb promoter activity in murine hematopoietic stem cells (HSCs), bone marrow (BM), and lymphocytes, an H2Kb enhancer/promoter EGFP construct was used to generate transgenic mice. These mice demonstrated pancellular EGFP expression in both primitive BM Sca-1+Lin-Kit+ cells and side population (SP) cells. Additionally, all peripheral blood leukocytes subsets, erythrocytes, and platelets uniformly expressed EGFP strongly. Competitive BM transplantation assays established that transgenic H2Kb-EGFP HSCs had activity equivalent to wildtype HSCs in their ability to reconstitute hematopoiesis in lethally irradiated mice. In addition, immunohistochemistry revealed EGFP transgene expression in all tissues examined. This transgenic strain should be a useful reagent for both murine hematopoiesis studies and functional studies of specific cell types from particular tissues.
Dominici
Massimo
M
Division of Experimental Hematology, Department of Hematology and Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Tadjali
Merhdad
M
Kepes
Steven
S
Allay
Esther R
ER
Boyd
Kelli
K
Ney
Paul A
PA
Horwitz
Edwin
E
Persons
Derek A
DA
D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000662:veterinary; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic
D049452:Green Fluorescent Proteins