Structural characterization of human elastin derived peptides containing the GXXP sequence.
The degradation of elastin, the insoluble biopolymer of tropoelastin, can lead to the production of small peptides. These elastin-derived peptides (EDPs) are playing a key role in cellular behavior within the extracellular matrix, showing a great variety of biological effects such as chemotaxis, stimulation of cell proliferation, ion flux modifications, vasorelaxation, and inflammatory enzymes secretion. It has also been demonstrated recently that EDPs containing the GXXPG motif could induce pro-MMP1 and pro-MMP3 upregulation. Elastolysis could then cause collagen degradation and play an important role in the aging process. Many experimental studies have been devoted to EDPs, but their structure/activity relationships are not well elucidated yet. However, the assumption that their active conformation is a type VIII beta-turn on GXXP was highly suggested on the basis of predictive statistical calculations. Investigation of the EDPs three-dimensional (3D) structure would provide useful information for drug-design strategies to propose specific inhibitors. The work presented here reports theoretical results obtained from molecular dynamics simulations performed over 128 human EDPs containing the GXXP motif. We show that all the peptides, for which the central residues are not glycines, adopt a canonical (or very close to) type VIII beta-turn structure on the GXXP sequence. Amino acids surrounding this motif are also important for the structural behavior. Any residue located before the GXXP motif (XGXXP) increases the beta-turn stabilization, whereas the residue located after GXXP (GXXPX) has no significant structural effect. Moreover, we show their biological activity can be correlated with their ability to exhibit a type VIII beta-turn conformation.
Moroy
Gautier
G
Laboratoire de Spectroscopies et Structures BioMoléculaires (LSSBM), IFR 53 Biomolécules, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims Cedex 2, France.
Alix
Alain J P
AJ
Héry-Huynh
Stéphanie
S
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D003198:Computer Simulation; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism*; D005998:Glycine / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000502:physiology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D014333:Tropoelastin / Q000737:chemistry
D010455:Peptides; D014333:Tropoelastin; D004549:Elastin; D005998:Glycine