title: Astrocyte-associated axonal damage in pre-onset stages of experimental autoimmune encephalomyelitis.
abstract: Recent studies of axon-glia and glia-glia communication have emphasized interactivity and interdependence between central nervous system (CNS) components. Concurrently, data from imaging, biochemical, and morphological studies have changed the view of multiple sclerosis (MS) from a neuroinflammatory condition with primary demyelination to one in which cumulative axonal damage drives progression. We therefore studied axonal damage in the context of inflammation and glial responses, from the pre-clinical to onset stage of murine experimental autoimmune encephalomyelitis (EAE), an established MS model. We report three major findings: (1) the first evidence of axonal injury before significant T-cell entry into the parenchyma, (3) coincidence of the earliest manifestation of axonal damage and astrocytic responses, and (3) an association between accumulation of axonal and astrocytic changes and specific forms of MS. These data demonstrate the relationship between the initiation of axonal injury and early inflammation. Significantly, we show that, in common with a growing number of neurodegenerative conditions, the pathology of murine EAE is characterized by early active contribution from astrocytes. This marks a change in the understanding of the role of astrocytes in MS pathogenesis and has important implications for the development of neuroprotective strategies.
authors.affiliation: Department of Biochemistry, Neuroimmunology Laboratory, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Wang
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Ayers
authors.forename: Deanne V
authors.initials: DV
authors.lastname: Catmull
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Hazelwood
authors.forename: Claude C A
authors.initials: CC
authors.lastname: Bernard
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Orian
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000648:ultrastructure; D001369:Axons / Q000473:pathology* / Q000648:ultrastructure; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D002490:Central Nervous System / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D004195:Disease Models, Animal; D018450:Disease Progression; D004660:Encephalitis / Q000473:pathology / Q000503:physiopathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D005911:Gliosis / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D046529:Microscopy, Electron, Transmission; D009103:Multiple Sclerosis / Q000473:pathology / Q000503:physiopathology; D009186:Myelin Sheath / Q000473:pathology / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors; D014855:Wallerian Degeneration / Q000473:pathology / Q000503:physiopathology*
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins