title: LC-PDA and LC-ESI-MS separation and determination of process-related substances arising from stilbene-type fluorescent whitening agents. Application to monitoring of their photodegradation products in industrial effluents and aqueous environmental systems.
abstract: A simple and rapid gradient elution high-performance liquid chromatographic method using photodiode array and electrospray ionization mass spectrometric detectors was developed for separation and determination of the process-related substances and photodegradation products of stilbenesulfonic acids, viz. 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNSDA), 4-amino-4'-nitrostilbene-2,2'-disulfonic acid (ANSDA), and 4,4'-diaminostilbene-2,2'-disulfonic acid (DASDA) in industrial waste waters. Gradient elution was carried out using ammonium acetate and acetonitrile as mobile phase and an Inertsil-ODS 3V column for separation. The negative-ion electrospray ionization mass spectra containing [M-H]- ions of sulfonic acids allowed molecular mass determination of unknowns and the structures were proposed on the basis of the fragment ions in the MS/MS spectra.
authors.affiliation: Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad-500 007, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: N
authors.initials: N
authors.lastname: Venkateswarlu
authors.forename: Sara
authors.initials: S
authors.lastname: Khalid
authors.forename: R
authors.initials: R
authors.lastname: Narsimha