T cell costimulation by chemokine receptors.
Signals mediated by chemokine receptors may compete with T cell receptor stop signals and determine the duration of T cell-antigen-presenting cell interactions. Here we show that during T cell stimulation by antigen-presenting cells, T cell chemokine receptors coupled to G(q) and/or G(11) protein were recruited to the immunological synapse by a G(i)-independent mechanism. When chemokine receptors were sequestered at the immunological synapse, T cells became insensitive to chemotactic gradients, formed more stable conjugates and finally responded with enhanced proliferation and cytokine production. We suggest that chemokine receptor trapping at the immunological synapse enhances T cell activation by improving T cell-antigen-presenting cell attraction and impeding the 'distraction' of successfully engaged T cells by other chemokine sources.
Molon
Barbara
B
Venetian Institute of Molecular Medicine and Department of Biomedical Science, University of Padua, 35100 Padua, Italy.
Gri
Giorgia
G
Bettella
Monica
M
Gómez-Moutón
Concepción
C
Lanzavecchia
Antonio
A
Martínez-A
Carlos
C
Mañes
Santos
S
Viola
Antonella
A
D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D011485:Protein Binding; D019707:Receptors, Chemokine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
D019707:Receptors, Chemokine; D043802:GTP-Binding Protein alpha Subunits, Gq-G11