Gram-scale synthesis and biofunctionalization of silica-coated silver nanoparticles for fast colorimetric DNA detection.
A direct silica-coating method has been developed for the gram-scale synthesis of well-dispersed Ag@SiO(2) nanoparticles. Subsequent surface functionalization via the well-established silica surface chemistry provided arching points for straightforward bioconjugation with amino-terminated oligonucleotides. Fast hybridization kinetics of the resulting robust oligo-modified Ag@SiO(2) nanoprobes with complementary target oligonucleotides render themselves very useful for the fast colorimetric DNA detection based on the sequence-specific hybridization properties of DNA. Additionally, the reliable protocols developed in this study for preparing and functionalizing Ag@SiO(2) nanoparticles can be readily extended to other silica-coated nanoparticles, which can also provide a specific platform for the covalent attachment of biomolecules such as amino-rich proteins, enzymes, or amino-terminated oligonucleotides for diverse bioapplications.
Liu
Shuhua
S
Institute of Materials Research and Engineering, 3 Research Link, Singapore 117602.
Zhang
Zhihua
Z
Han
Mingyong
M
D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000032:analysis*; D017403:In Situ Hybridization; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D012822:Silicon Dioxide; D012834:Silver; D018030:Silver Compounds / Q000138:chemical synthesis*; D013499:Surface Properties; D013696:Temperature
D009841:Oligonucleotides; D018030:Silver Compounds; D012834:Silver; D012822:Silicon Dioxide; D004247:DNA