title: How restriction enzymes became the workhorses of molecular biology.
abstract: Restriction enzymes have proved to be invaluable for the physical mapping of DNA. They offer unparalleled opportunities for diagnosing DNA sequence content and are used in fields as disparate as criminal forensics and basic research. In fact, without restriction enzymes, the biotechnology industry would certainly not have flourished as it has. The first experiments demonstrating the utility of restriction enzymes were carried out by Danna and Nathans and reported in 1971. This pioneering study set the stage for the modern practice of molecular biology in which restriction enzymes are ubiquitous tools, although they are often taken for granted.
authors.affiliation: New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA. roberts@neb.com
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Roberts
mesh_terms: D001483:Base Sequence; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D049674:History, 21st Century; D008967:Molecular Biology / Q000379:methods*; D009613:Nobel Prize; D015183:Restriction Mapping; D014481:United States
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes