title: Teicoplanin-dependent antibodies: detection and characterization.
abstract: There are only a few reports of thrombocytopenia associated with clinical doses of teicoplanin, a glycopeptide antibiotic used against Gram-positive bacteria. We investigated 39 patients receiving teicoplanin; 31 were thrombocytopenic with platelet counts between 1-105 x 10(9)/l and 8 were not thrombocytopenic. We identified 14 thrombocytopenic cases (45%) and two (25%) non-thrombocytopenic cases with IgG teicoplanin-dependent platelet-reactive antibodies. Use of glycoprotein (GP) capture enzyme-linked immunosorbent assay with platelets and GPIIb/IIIa transfected Chinese Hamster Ovary cells as well as flow cytometry with GP-deficient platelets indicated that the GPIIb/IIIa complex is a major target antigen of these antibodies.
authors.affiliation: National Blood Service, Cambridge, UK. sfg21@cam.ac.uk
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Garner
authors.forename: Kate
authors.initials: K
authors.lastname: Campbell
authors.forename: Graham
authors.initials: G
authors.lastname: Smith
authors.forename: Colin
authors.initials: C
authors.lastname: Hurd
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Davidson
authors.forename: Marilyn
authors.initials: M
authors.lastname: Treacy
authors.forename: John F
authors.initials: JF
authors.lastname: Burman
authors.forename: Hartmut
authors.initials: H
authors.lastname: Kroll
authors.forename: Willem H
authors.initials: WH
authors.lastname: Ouwehand
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000906:Antibodies / Q000097:blood*; D000937:Antigen-Antibody Reactions; D001792:Blood Platelets / Q000276:immunology; D016466:CHO Cells; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D017334:Teicoplanin / Q000276:immunology*; D013921:Thrombocytopenia / Q000139:chemically induced*
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D007074:Immunoglobulin G; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D017334:Teicoplanin