Isolation of lymphocystis disease virus from sole, Solea senegalensis Kaup, and blackspot sea bream, Pagellus bogaraveo (Brunnich).
Two viruses were isolated from cultured sole, Solea senegalensis, and wild blackspot sea bream, Pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (LCDVs). Viral isolates were characterized by morphological, biochemical and biophysical properties. In addition, the susceptibility of four fish cell lines was also tested. LCDV isolates developed cytopathic effects on the SAF-1 cell line at 5 and 6 days post-infection and reached titres of 10(6) TCID50 mL(-1). The antigenic and structural protein analysis of the two new LCDV isolates showed identical profiles to that obtained for LCDV strain Leetown NFH (ATCC VR-342), used as a reference viral strain, and for an LCDV isolate collected from gilt-head sea bream, Sparus aurata, cultured in southern Spain. Molecular confirmation was performed by polymerase chain reaction. Specific primers for LCDV produced a 270-bp DNA fragment, the expected size for LCDV.
Alonso
M C
MC
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos, Málaga, Spain.
Cano
I
I
Garcia-Rosado
E
E
Castro
D
D
Lamas
J
J
Barja
J L
JL
Borrego
J J
JJ
Bergmann
S M
SM
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers; D004266:DNA Virus Infections / Q000662:veterinary*; D004198:Disease Susceptibility / Q000662:veterinary / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000821:virology*; D005412:Flatfishes; D007497:Iridoviridae / Q000302:isolation & purification*; D010473:Perciformes; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014764:Viral Proteins / Q000302:isolation & purification
D000914:Antibodies, Viral; D017931:DNA Primers; D014764:Viral Proteins