Multiplex PCR for typing strains of Toxoplasma gondii.
A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.
Ajzenberg
Daniel
D
Laboratoire de Parasitology-Mycologie, Faculté de Médecine EA 3174, Limoges, France.
Dumètre
Aurélien
A
Dardé
Marie-Laure
ML
D000818:Animals; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics