[Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].
OBJECTIVE
To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats.


METHODS
The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation.


RESULTS
In 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05).


CONCLUSIONS
PDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.
Tan
Qian
Q
Department of Burns and Plastic Surgery, Drum Tower Hospital, Medical College of Nanjing University, Nanjing, China.
Chen
Xi
X
Liang
Zhi-wei
ZW
Huang
Pei-lin
PL
Zhou
Hong-reng
HR
Yang
Ding-wen
DW
Lin
Zi-hao
ZH
Jiang
Hua
H
D062088:Acellular Dermis; D000818:Animals; D008297:Male; D018919:Neovascularization, Physiologic; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply*; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection
D020574:Proto-Oncogene Proteins c-sis