Peptidergic contribution to posttetanic potentiation at a central synapse of aplysia.
Posttetanic potentiation (PTP)-like phenomena appear to be mediated by a variety of mechanisms. Although neuropeptides are located in a large number of neurons and many neuropeptides, like PTP, can enhance synaptic transmission, there is a paucity of studies indicating that peptides may actually participate in PTP. Here, we utilize a single central synapse in the feeding circuit of Aplysia to investigate a possible peptidergic contribution to PTP in the CNS. The cholinergic command-like interneuron, cerebral-buccal interneuron 2 (CBI-2), contains two neuropeptides, feeding circuit activating peptide (FCAP) and cerebral peptide 2 (CP2). Previous studies showed that tetanic prestimulation or repeated stimulation of CBI-2, as well as perfusion of FCAP and CP2, increase the size of the cholinergic excitatory postsynaptic potentials (EPSPs) that CBI-2 evokes in the motoneurons B61/62 and shorten the latency to initiate B61/62 firing in response to CBI-2 stimulation. We used temperature-dependent suppression of peptide release and occlusion experiments to examine the possible contribution of FCAP and CP2 to PTP at the CBI-2 to B61/62 synapse. When peptide release was suppressed, perfusion of exogenous peptides increased the size of posttetanic EPSPs. In contrast, when peptide release was not suppressed, exogenous peptides did not enhance the size of posttetanic EPSPs, thus indicating occlusion. Temperature manipulation and occlusion experiments also indicated that peptides extend PTP duration. This peptide-dependent prolongation of PTP has functional consequences in that it extends the duration of time during which the latency to initiate B61/62 firing in response to CBI-2 stimulation is shortened.
Koh
Hae-Young
HY
Department of Neuroscience, Mount Sinai School of Medicine, New York, NY 10029, USA.
Weiss
Klaudiusz R
KR
D000818:Animals; D001048:Aplysia / Q000502:physiology*; D002490:Central Nervous System / Q000166:cytology / Q000502:physiology; D004347:Drug Interactions; D004558:Electric Stimulation / Q000379:methods; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D009479:Neuropeptides / Q000494:pharmacology*; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D013569:Synapses / Q000145:classification / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D009435:Synaptic Transmission / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D009479:Neuropeptides; C103537:cerebral peptide 2