Effect of different immunosuppressive therapies on the lipid pattern in kidney-transplanted rats.
We analyzed the effect of oral administration of cyclosporine-methylprednisone (CsA-MP) and sirolimus (SRL) on the lipid pattern of kidney-transplanted rats after a 7-day survival. A significant increase in plasma cholesterol in CsA-MP group (control: 26 +/- 3 mg/dl vs. 59 +/- 8 mg/dl, P < 0.05) and in triglyceride levels in SRL group (control: 53 +/- 4 mg/dl vs. 114 +/- 3 mg/dl, P < 0.05), was shown. Kidney microsomal membranes from both treated groups showed that cholesterol and triglyceride values and the relative percentage of arachidonic acid in the total amount of n-6 fatty acids decreased. A diminution of linoleic acid occurred in testis (control: 9.4 +/- 0.1 mg/dl vs. CsA-MP: 6.0 +/- 0.3 mg/dl and vs. SRL: 6.8 +/- 0.2 mg/dl, P < 0.05), liver (control: 17.7 +/- 0.6 mg/dl vs. CsA-MP: 15.1 +/- 0.6 mg/dl and SRL: 13.5 +/- 0.8 mg/dl, P < 0.05) and erythrocyte membranes (control:11.7 +/- 0.1% vs. CsA-MP: 10.6 +/- 0.2% and SRL: 10.0 +/- 0.4%, P < 0.01). The immunosuppressive therapies improved the rejection rate of the graft, fact that was remarkable in the SRL-treated group. However, lipid abnormalities still remain in spite of immunosuppressive therapies (150).
Lausada
Natalia
N
Laboratorio de Trasplante de Organos, Facultad de Ciencias Médicas, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP-CONICET-UNLP), Universidad Nacional de La Plata, La Plata, Argentina. nlausada@atlas.med.unlp.edu.ar
de Gómez Dumm Nelva
T
T
Georgina
Luna
L
Gisela
Camihort
C
Clemente
Raimondi
R
D000818:Animals; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000633:toxicity; D004910:Erythrocyte Membrane / Q000378:metabolism; D006949:Hyperlipidemias / Q000139:chemically induced; D007166:Immunosuppressive Agents / Q000633:toxicity*; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology / Q000502:physiology*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008775:Methylprednisolone / Q000633:toxicity; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020123:Sirolimus / Q000633:toxicity; D013737:Testis / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014508:Urea / Q000097:blood
D007166:Immunosuppressive Agents; D008055:Lipids; D008563:Membrane Lipids; D014280:Triglycerides; D016572:Cyclosporine; D014508:Urea; D002784:Cholesterol; D003404:Creatinine; D020123:Sirolimus; D008775:Methylprednisolone