title: Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells.
abstract: Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
authors.affiliation: Molecular Medicine Program, Mayo Clinic, Guggenheim 1838, 200 1st Street SW, Rochester, MN 55905, USA.
authors.forename: Veronika
authors.initials: V
authors.lastname: von Messling
authors.forename: Numan
authors.initials: N
authors.lastname: Oezguen
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Sompong
authors.initials: S
authors.lastname: Vongpunsawad
authors.forename: Werner
authors.initials: W
authors.lastname: Braun
authors.forename: Roberto
authors.initials: R
authors.lastname: Cattaneo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D002478:Cells, Cultured; D004217:Distemper Virus, Canine / Q000502:physiology*; D005289:Ferrets; D006023:Glycoproteins; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology*; D007136:Immunoglobulins; D007963:Leukocytes, Mononuclear / Q000821:virology; D008561:Membrane Fusion; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014779:Virus Replication
chemical_list: D015703:Antigens, CD; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1