title: Oxidative stress and nitric oxide in rats with alcohol-induced acute pancreatitis.
abstract: OBJECTIVE
  Oxygen free radical mediated tissue damage is well established in pathogenesis of acute pancreatitis (AP). Whether nitric oxide (NO) plays a deleterious or a protective role is unknown. In alcohol-induced AP, we studied NO, lipooxidative damage and glutathione in pancreas, lung and circulation.
  
  
  METHODS
  AP was induced in rats (n = 25) by injection of ethyl alcohol into the common biliary duct. A sham laparatomy was performed in controls (n = 15). After 24 h the animals were killed, blood and tissue sampling were done.
  
  
  RESULTS
  Histopathologic evidence confirmed the development of AP. Marked changes were observed in the pulmonary tissue. Compared with controls, the AP group displayed higher values for NO metabolites in pancreas and lungs, and thiobarbituric acid reactive substances in circulation. Glutathione was lower in pancreas and in circulation. Glutathione and NO were positively correlated in pancreas and lungs of controls but negatively correlated in circulation of experimental group. In the experimental group, plasma thiobarbituric acid reactive substances were negatively correlated with pancreas thiobarbituric acid reactive substances but positively correlated with pancreas NO.
  
  
  CONCLUSIONS
  NO increases in both pancreas and lungs in AP and NO contributes to the pathogenesis of AP under oxidative stress.
authors.affiliation: Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. andican@e-kolay.net
authors.forename: Gülnur
authors.initials: G
authors.lastname: Andican
authors.forename: Remisa
authors.initials: R
authors.lastname: Gelisgen
authors.forename: Ethem
authors.initials: E
authors.lastname: Unal
authors.forename: Osman-Baran
authors.initials: OB
authors.lastname: Tortum
authors.forename: Sergülen
authors.initials: S
authors.lastname: Dervisoglu
authors.forename: Tayfun
authors.initials: T
authors.lastname: Karahasanoglu
authors.forename: Gülden
authors.initials: G
authors.lastname: Burçak
mesh_terms: D000208:Acute Disease; D000437:Alcoholism / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D005978:Glutathione / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010195:Pancreatitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D005978:Glutathione