title: N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay.
abstract: A novel caspase-3 substrate N-Ac-DEVD-N'-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.
authors.affiliation: Department of Reagent and Assay Development, Molecular Devices Corporation, 1311 Orleans Drive, Sunnyvale, CA 94089, USA. zhiqiang_wang@moldev.com
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Jinfang
authors.initials: J
authors.lastname: Liao
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Diwu
mesh_terms: D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D009025:Morpholines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013379:Substrate Specificity
chemical_list: D007202:Indicators and Reagents; D009025:Morpholines; C504180:N-DEVD-N'-morpholinecarbonyl-rhodamine 110; D009842:Oligopeptides; D011994:Recombinant Proteins; D012235:Rhodamines; C049025:rhodamine 110; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases