Identification of differential gene expression in germinal vesicle vs. metaphase II mouse oocytes by using annealing control primers.
By using a new innovative technology, annealing control primer-polymerase chain reaction (ACP-PCR), we identified genes that are differentially expressed in immature GV vs. in mature MII mouse oocytes. The results of the present study will be valuable in understanding the components of oocyte maturation and provide a basis for studies of human oocyte maturation.
Yoon
Se-Jin
SJ
CHA Research Institute, Pochon CHA University College of Medicine, 606-5 Yeoksam 1-dong, Gangnam-gu, Seoul 135-081, South Korea.
Chung
Hyung-Min
HM
Cha
Kwang-Yul
KY
Kim
Nam-Hyung
NH
Lee
Kyung-Ah
KA
D000818:Animals; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D008677:Metaphase / Q000235:genetics; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers