Dihydrofolate reductase in Pediococcus cerevisiae strains susceptible and resistant to amethopterin.
A mutant strain of Pediococcus cerevisiae was obtained which had 1,000-fold greater resistance to amethopterin than the P. cerevisiae/PteGlu strain. The dihydrofolate reductase level of the mutant was about 60-fold higher than that of the parent. The mutant accumulated folate, though less efficiently than its parent.
Mandelbaum-Shavit
F
F
Grossowicz
N
N
D024881:Drug Resistance, Bacterial; D008727:Methotrexate / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010376:Pediococcus / Q000187:drug effects* / Q000201:enzymology*; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*
D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate