Cloning and sequencing of hydroxylase genes involved in taxol biosynthesis.
Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis. These findings imply that the two new genes are closely related to the biosynthesis of taxol/taxoids. Data Accession No: AF545833 and AY374652.
Tu
Jun
J
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, P R China. zhuping@imm.ac.cn
Zhu
Ping
P
Cheng
Ke-di
KD
Meng
Chao
C
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020946:Taxus / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D006899:Mixed Function Oxygenases; D017239:Paclitaxel