title: [Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].
abstract: OBJECTIVE
  To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats.
  
  
  METHODS
  The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation.
  
  
  RESULTS
  In 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05).
  
  
  CONCLUSIONS
  PDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.
authors.affiliation: Department of Burns and Plastic Surgery, Drum Tower Hospital, Medical College of Nanjing University, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Liang
authors.forename: Pei-lin
authors.initials: PL
authors.lastname: Huang
authors.forename: Hong-reng
authors.initials: HR
authors.lastname: Zhou
authors.forename: Ding-wen
authors.initials: DW
authors.lastname: Yang
authors.forename: Zi-hao
authors.initials: ZH
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D008297:Male; D018919:Neovascularization, Physiologic; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply*; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection
chemical_list: D020574:Proto-Oncogene Proteins c-sis