title: The relationship between bone metastasis from human breast cancer and integrin alpha(v)beta3 expression.
abstract: BACKGROUND
  Osteoclast activation plays an essential role in the development of bone metastasis (BM). Integrin alpha(v)beta3 mediates the attachment of osteoclasts to bone matrix, and it is overexpressed in bone-residing breast cancer cells. We studied BM from breast cancer in relation to its Integrin alpha(v)beta3 expression.
  
  
  METHODS
  Integrin alpha(v)beta3 expression in 4 human breast cancer cell lines (MDA-MB-231, MDA-MB-435, MKL-4 and T-47D) was determined by immunohistochemistry, flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Integrin alpha(v)beta3 was expressed in MDA-MB-231 and MDA-MB-435, but not in MKL-4 or T-47D, using both immunohistochemistry and flow cytometry. By RT-PCR, positive bands for both alpha(v) and beta3 subunits were identified in MDA-MB-231 and 435, while only the alpha(v) subunit mRNA was present in MKL-4 and T-47D. BM was common in vivo following inoculation with MDA-MB-231 or 435, but not with MKL-4.
  
  
  CONCLUSIONS
  Integrin alpha(v)beta3 expression appears to play a key role in the development of BM from breast cancer.
authors.affiliation: Department of Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. stakayam@sc.itc.keio.ac.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Takayama
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Ishii
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Masamura
authors.forename: Masakazu
authors.initials: M
authors.lastname: Doi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000556:secondary*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D039302:Integrin alphaVbeta3 / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous
chemical_list: D039302:Integrin alphaVbeta3