title: Pilot Anopheles gambiae full-length cDNA study: sequencing and initial characterization of 35,575 clones.
abstract: We describe the preliminary analysis of over 35,000 clones from a full-length enriched cDNA library from the malaria mosquito vector Anopheles gambiae. The clones define nearly 3,700 genes, of which around 2,600 significantly improve current gene definitions. An additional 17% of the genes were not previously annotated, suggesting that an equal percentage may be missing from the current Anopheles genome annotation.
authors.affiliation: Unité de Biochimie et Biologie Moléculaire des Insectes and CNRS FRE 2849, Institut Pasteur, 75724 Paris Cedex 15, France. sgomez@pasteur.fr
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Gomez
authors.forename: Karin
authors.initials: K
authors.lastname: Eiglmeier
authors.forename: Beatrice
authors.initials: B
authors.lastname: Segurens
authors.forename: Pierre
authors.initials: P
authors.lastname: Dehoux
authors.forename: Arnaud
authors.initials: A
authors.lastname: Couloux
authors.forename: Claude
authors.initials: C
authors.lastname: Scarpelli
authors.forename: Patrick
authors.initials: P
authors.lastname: Wincker
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
authors.forename: Paul T
authors.initials: PT
authors.lastname: Brey
authors.forename: Charles W
authors.initials: CW
authors.lastname: Roth
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001482:Base Composition / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010865:Pilot Projects; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D010457:Peptidoglycan