Comparison of X-ray fluorescence and optical fluorescence measured behind scattering layers as a basis for X-ray fluorescence endoscopy.
Recent developments in the technology of capillary-fiber optics suitable for X-rays in the range of approximately 4-10keV point to the possible realization of endoscopes applicable in X-ray fluorescence analysis. A general problem is the determination of scattering and absorption processes with consideration to tissue optics, X-ray scattering and X-ray absorption in a diagnostic partial volume. Therefore comparative investigations were performed in order to answer these questions. Zinc-oxide nanoparticles configured as single particles and ZnO clusters provided the fluorescence source in cell layers. An artificial scattering material was employed, which closely approximated the tissue optical conditions and the X-ray optical application conditions in possible diagnostic situations. As a result imaging of spatially resolved X-ray contrasts was better than adequate optical fluorescence imaging by approximately one magnitude. Hence a very important precondition for realizing X-ray fluorescence endoscopy is fulfilled.
Beuthan
J
J
Institut für Medizinische Physik und Lasermedizin, Charité Campus Benjamin Franklin, Universitätsmedizin - Berlin, Fabeckstrasse 60-62, 14195 Berlin. j.beuthan@charite.de
Haschke
M
M
Dressier
C
C
Wacker
F
F
D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000648:ultrastructure*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*