[Study of gene differential display of hydatidiform moles].
OBJECTIVE
To screen the specific molecular maker of invasive hydatidiform moles (HM) by differential display analysis.


METHODS
For dot hybridization, about 1.0 microg of each cDNA sample of invasive and non-invasive HM were labeled as probes using the Dig DNA labeling and Detection Kit (Boehringer Mannheim). The specific expression fragments of invasive HM recovered from PAGE gel were re-amplified by PCR, and the PCR products were dotted onto nylon membrane and hybridized by two probes of invasive and non-invasive HM cDNA respectively. Some fragments with a strong positive hybridization signal were cloned into the polylinker of lasmid PUC19 and were sequenced. The fragments' sequences were searched for homology in the NCBI data using the BLAST (Database: GenBank Human EST entries; Posted date:Aug 31, 2004; Number of letters in database: 1,697,659,032; Number of sequences in database: 3,677,722).


RESULTS
The 20 fragments in 28 bands with specific expression in invasive HM were re-amplified, of which 13 showed positive hybridization signals, and 3 were cloned into the polylinker of lasmid PUC19. A fragment in the 3 was a new expressed sequence tag (EST) and the sequence was submitted to NCBI data (dbEST_Id: 10875704; GenBank_Accn: BM403211).


CONCLUSIONS
There are more differences in gene expression between invasive and non-invasive HMs, and differential display analyses are of a potential significance to early diagnosis of invasive HM.
Zhang
Xiao-wei
XW
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China. zhangxw@mail.bjmu.edu.cn
Zhu
Hao-bin
HB
Wu
Shu-ying
SY
Gao
Rong-lian
RL
Zhang
Lu-fang
LF
Han
Jin-song
JS
Guo
Hong-yan
HY
Wang
Xiu-yun
XY
Ye
Rong-hua
RH
Zhao
Yang-yu
YY
Zhao
Wen-qiu
WQ
D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics; D002820:Hydatidiform Mole, Invasive / Q000235:genetics*; D008969:Molecular Sequence Data; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000235:genetics*
D014408:Biomarkers, Tumor