[The methylation pattern of LRP15 gene in acute leukemia].
OBJECTIVE
To investigate the methylation status of LRP15 gene in acute leukemia (AL) and its role in tumorigenesis.


METHODS
73 cases of AL and 9 healthy subjects as well as COS7, K562 and HL60 cell lines were studied with methylation specific PCR (MSP).


RESULTS
LRP15 was not detected in all the samples. No LRP15 methylation was detected in COS7, but LRP15 was methylated in K562 and HL60. In nearly all of the French-American-British leukemia subtypes, we found that the frequency of LRP15 methylation was 71.2% (52/73) of AL and none in the 9 healthy subjects. The difference in mean methylation for LRP15 between these two kinds of samples is statistically significant (P < 0.05). Hypermethylation of the LRP15 gene was found in 57.1% (16/28) of the newly diagnosed AL and 83.3% of the relapsed AL respectively; this is also statistically significant (P < 0.05).


CONCLUSIONS
LRP15 methylation change is a common abnormality in leukemia and LRP15 is postulated to be a tumor suppressor gene.
Dou
Li-ping
LP
Department of Hematology, PLA General Hospital, Beijing 100853, China. lipingdou@yahoo.com.cn
Yu
Li
L
Lü
Qing
Q
Xu
Zhou-min
ZM
Liu
Hai-chuan
HC
Lou
Fang-ding
FD
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D019556:COS Cells; D019175:DNA Methylation; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D011506:Proteins / Q000235:genetics*
C477813:LRRC3B protein, human; D009363:Neoplasm Proteins; D011506:Proteins