Granulocyte macrophage-colony stimulating factor improves impaired anastomotic wound healing in rats treated with intraperitoneal mitomycin-C.
OBJECTIVE
Intraperitoneal chemotherapy (IPCT) delivers higher local concentrations of cytotoxic drugs than intravenous (i.v.) chemotherapy, but it can adversely affect the healing of intestinal anastomoses if given in the early postoperative period. Intestinal anastomotic leakage is a serious surgical complication. Experimental and clinical studies have shown that the local administration of granulocyte macrophage-colony stimulating factor (GM-CSF) improves would healing. Therefore, we evaluated the effects of locally applied GM-CSF on anastomotic wound healing in rats treated with intraperitoneal mitomycin-C immediately after surgery.


METHODS
We performed colon anastomoses in albino rats, which were then divided into three treatment groups. Group A was a control group that received no treatment, Group B was given intraperitoneal mitomycin-C postoperatively, and Group C was given intraperitoneal mitomycin-C with a local injection of GM-CSF postoperatively. We measured bursting pressures and hydroxyproline content, and histologically examined the resected anastomoses on postoperative day (POD) 3.


RESULTS
Anastomotic healing was impaired after intraperitoneal mitomycin-C, but this was overcome by the injection of GM-CSF into the perianastomotic area.


CONCLUSIONS
Local GM-CSF administration counteracts the detrimental effects of intraperitoneal mitomycin-C treatment on intestinal anastomoses in rats.
Cetinkaya
Kadir
K
Department of General Surgery, Ankara Oncology Education and Research Hospital, Ankara, Turkey.
Dinc
Soykan
S
Gulcelik
Mehmet Ali
MA
Renda
Nurten
N
Ustun
Huseyin
H
Caydere
Muzaffer
M
Alagol
Haluk
H
D000704:Analysis of Variance; D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000503:physiopathology / Q000601:surgery*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D016685:Mitomycin / Q000633:toxicity*; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects*
D016685:Mitomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor