title: Deamination of adenosine by extracts of Penicillium politans NRC-510.
abstract: Cell-free extracts of nitrate-grown Penicillium politans NRC-510 could catalyze the hydrolytic deamination of adenosine to inosine maximally at pH 6.0 and 45 degrees C. However the same extracts could not catalyze the N-glycosidic bond cleavage of adenosine at pH 4.0, 6.0 and 8.0. Incubation of the extracts at 55 degrees C for 30 minutes caused about 31% loss in activity whereas incubation of the extracts at 60 degrees C for 15 minutes caused a complete loss of enzyme activity. Results indicated the absence of the involvement of sulfhydryl groups in the catalytic site of adenosine deaminase. The enzyme is inhibited by ethylene diamine tetraacetate indicating that adenosine deaminase is a metalloenzyme. MnCl2 and MgCl2 had a remarkable activating effect, whereas HgCl2, CaCl2 and ZnSO4 showed an inhibitory effect on enzyme activity. Dialyzing the extracts for 24 hours significantly increase deaminase activity by about 33%. The apparent K(m) value was calculated for adenosine and found to be 3.63 x 10(-3) M, which indicates high affinity of adenosine deaminase for its substrate adenosine.
authors.affiliation: Department of Microbial Chemistry, National Research Centre, El-Tahrir Street, Dokki, Cairo, Egypt. alielshafei@yahoo.com
authors.forename: Ali M
authors.initials: AM
authors.lastname: Elshafei
authors.forename: Latifa A
authors.initials: LA
authors.lastname: Mohamed
authors.forename: Nadia H
authors.initials: NH
authors.lastname: Ali
mesh_terms: D000241:Adenosine / Q000737:chemistry / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism*; D003641:Deamination; D004492:Edetic Acid; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D004492:Edetic Acid; D000243:Adenosine Deaminase; D000241:Adenosine