title: Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration.
abstract: The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation-vitrification (EnVi) and encapsulation-dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, respectively). In both cryogenic procedures, shoots regenerated from cryopreserved shoot tips without intermediary callus formation. Histological studies showed that a much larger number of meristematic cells survived following EnVi than EnDe. The EnVi procedure was applied to seven raspberry genotypes with an average survival and regrowth of 71 and 68%, respectively. Regenerated plants showed normal morphology. Results here indicate EnVi as a simple and efficient method for long-term preservation of R. idaeus germplasm.
authors.affiliation: Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland.
authors.forename: Qiaochun
authors.initials: Q
authors.lastname: Wang
authors.forename: Jaana
authors.initials: J
authors.lastname: Laamanen
authors.forename: Marjatta
authors.initials: M
authors.lastname: Uosukainen
authors.forename: Jari P T
authors.initials: JP
authors.lastname: Valkonen
mesh_terms: D015925:Cryopreservation; D018520:Plant Shoots / Q000254:growth & development; D027824:Rosaceae / Q000254:growth & development