[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].
OBJECTIVE
To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA).


METHODS
Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit.


RESULTS
The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%;-6.2%;, and coefficient of variability between days of 5.3%;-9.6%;. The results of detection were consistent between ELISA and TRFIA.


CONCLUSIONS
TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.
Wang
Lei
L
Institute of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Wu
Ying-Song
YS
Tang
Yong-Ping
YP
Li
Ming
M
D000911:Antibodies, Monoclonal / Q000276:immunology; D000086462:Coronavirus Nucleocapsid Proteins; D015200:Fluoroimmunoassay / Q000379:methods; D006801:Humans; D019590:Nucleocapsid Proteins / Q000032:analysis* / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000821:virology
D000911:Antibodies, Monoclonal; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins