[Determination of the content of serum calcium with methylthymol blue as chromogenic reagent].
It is reported in this paper that calcium can be determined with MTB as chromogenic reagent by isoabsorption dual-wavelength elimination. The measuring wavelength and referential wavelength were 613.7 and 590.0nm, respectively. No masking reagent was necessary in the analysis. The average recovery and RSD of calcium are 98.98% and 0.81%, respectively. Compared with titration, this method is rapid, simple and precise.
Yang
C
C
Pharmacy School of Hebei Medical University, 050017 Shijiazhuang.
Liu
W
W
Zhao
Z
Z
Wu
H
H
D000042:Absorption; D001774:Blood Chemical Analysis / Q000379:methods*; D001979:Bromthymol Blue / Q000031:analogs & derivatives* / Q000737:chemistry; D002118:Calcium / Q000097:blood*; D002138:Calibration; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; C002074:methylthymol blue; D002118:Calcium; D001979:Bromthymol Blue