Characterization of new miniSTR loci to aid analysis of degraded DNA.
A number of studies have demonstrated that successful analysis of degraded DNA specimens from mass disasters or forensic evidence improves with smaller sized polymerase chain reaction (PCR) products. We have scanned the literature for new STR loci, unlinked from the CODIS markers, which can generate amplicons less than 125 bp in size and would therefore be helpful in testing degraded DNA samples. New PCR primers were designed and tested for the STR loci D1S1677, D2S441, D4S2364, D10S1248, D14S1434, and D22S1045, arranged into two miniSTR triplexes. All loci show a moderate degree of polymorphism among 474 U.S. population samples tested and were reliable and sensitive to at least 100 pg of DNA template under controlled laboratory conditions and pristine DNA samples. The utility of these new loci were confirmed in comparing the success of the miniSTR assays for typing degraded bone samples while partial profiles were observed with the majority of the samples using a commercial STR kit.
Coble
Michael D
MD
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA. michael.coble@nist.gov
Butler
John M
JM
D001842:Bone and Bones; D004247:DNA / Q000032:analysis* / Q000378:metabolism*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA