Fabrication of quantum dot-lectin conjugates as novel fluorescent probes for microscopic and flow cytometric identification of leukemia cells from normal lymphocytes.
The present study describes a synthesis of QD-lectin conjugates and their application for identification of leukaemia cells from normal lymphocytes using fluorescent confocal microscopy and flow cytometry. The results are compared with commercially available FITC-lectin.
Zhelev
Zhivko
Z
Single-Molecule Bioanalysis Laboratory, AIST-Shikoku, 2217-14, Hayashi-cho, Takamatsu, Kagawa-ken, Japan.
Ohba
Hideki
H
Bakalova
Rumiana
R
Jose
Rajan
R
Fukuoka
Satoshi
S
Nagase
Toshimi
T
Ishikawa
Mitsuru
M
Baba
Yoshinobu
Y
D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D037102:Lectins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013677:Medical Laboratory Science; D018613:Microscopy, Confocal; D015394:Molecular Structure; D045663:Quantum Dots
D005456:Fluorescent Dyes; D037102:Lectins; D016650:Fluorescein-5-isothiocyanate