Low dose magnetic fields do not cause oxidative DNA damage in human placental cotyledons in vitro.
The biological impact of low dose magnetic fields generated by electric appliances present in the human environment is still uncertain. In this study, human placentas served as a model tissue for the evaluation of the potential effect of oscillating low intensity magnetic fields on the concentration of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in cellular DNA. Cotyledons were dissected from placentas obtained immediately after physiological labours and exposed to magnetic fields (groups MF A, 2 mT, 50 Hz and MF B, 5 mT, 50 Hz) or sham exposed (group C) during an in vitro perfusion of 3 h. Cellular DNA was isolated, hydrolyzed and analyzed by HPLC. Native nucleosides were monitored at 254 nm and 8-OH-dG by electrochemical detection. Results were expressed as mumol 8-OH-dG/mol deoxyguanosine (dG). The concentrations of 8-OH-dG in group C, MF A and MF B were 28.45+/-15.27 micromol/mol dG, 62.80+/-31.91 mumol/mol dG, and 27.49+/-14.23 micromol/mol dG, respectively, demonstrating no significant difference between the groups. The results suggest that placental tissues possess a capacity to protect DNA against oxidative alterations by magnetic field of intensities previously shown to produce radical mediated DNA damage in rat brain cells in vivo and imbalances in electrolyte release of cotyledons under in vitro conditions.
Lopucki
Maciej
M
I. Chair and Department of Obstetrics and Gynaecology, Faculty of Medicine, Medical University School, Staszica 16, 20-081, Lublin, Poland.
Schmerold
Ivo
I
Dadak
Agnes
A
Wiktor
Henryk
H
Niedermüller
Hans
H
Kankofer
Marta
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage / Q000528:radiation effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D018384:Oxidative Stress / Q000502:physiology*; D010920:Placenta / Q000528:radiation effects*; D011247:Pregnancy
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine