title: Phylogenetic and restriction fragment length polymorphism analyses of hemagglutinin (H) protein of canine distemper virus isolates from domestic dogs in Japan.
abstract: We conducted phylogenetic and restriction fragment length polymorphism (RFLP) analyses of 995 nucleotides within the hemagglutinin (H) gene open reading frame (ORF) of field isolates of 23 canine distemper virus (CDV) strains isolated from domestic dogs in Japan between 1982 and 1998. The phylogenetic analysis showed that Japanese field isolates could be separated into three groups. Eighteen out of the twenty-three strains constituted one cluster consisting of Japanese CDVs, four strains formed a second Japanese CDV group, and only one strain belonged to a group containing foreign CDV strains. By RFLP analysis using SspI, we could distinguish all the Japanese field isolates from the vaccine strains. Thus, the RFLP method is useful for differentiating the infections with field CDV strains from the vaccine strains in clinical cases.
authors.affiliation: Laboratory of Animal Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Uema
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Ohashi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Wakasa
authors.forename: Chieko
authors.initials: C
authors.lastname: Kai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000235:genetics*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D012367:RNA, Viral; C061568:endodeoxyribonuclease SspI; D015252:Deoxyribonucleases, Type II Site-Specific