Comparison of different culture methods on bacterial recovery in hemodialysis fluids.
To examine the culture method that could provide the highest bacterial recovery, 143 reverse osmosis water samples used in hemodialysis were collected for comparison of the media (Tryptic Soy Agar, TSA vs Reasoner's 2A Agar, R2A), the temperature (20 degrees C vs 37 degrees C), the duration of incubation (48-hour vs 7-day), and the culture technique (membrane filtration vs spread plate methods). The European Best Practice Guideline method, R2A at 20 degrees Cfor 7-day incubation provided higher bacterial recovery than the Association for the Advancement of Medical Instrumentation (AAMI) method, TSA at 37 degrees C for 48-hour incubation. The membrane filtration method gave better yield than the spread plate method. As such, the European Best Practice Guideline method in combination with the membrane filtration technique would be the culture method of choice for hemodialysis fluids.
Punakabutra
Napawan
N
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Nunthapisud
Pongpun
P
Pisitkun
Trairak
T
Tiranathanagul
Khajohn
K
Tungsanga
Kriang
K
Eiam-Ong
Somchai
S
D000362:Agar; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D046508:Culture Techniques; D004340:Drug Contamination; D005374:Filtration; D015312:Hemodialysis Solutions; D006801:Humans; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D015312:Hemodialysis Solutions; D000362:Agar