title: [Cloning of human obesity gene and its expression in E. coli].
abstract: OBJECTIVE
  To clone the obesity gene of Chinese and express human leptin in E.coli.
  
  
  METHODS
  The obesity gene was amplified from the total RNA isolated from cultured human adipocytes of Chinese by reverse transcriptional PCR, inserted into TA-vector and cloned into the expression plasmid pBV220 after sequence identification.
  
  
  RESULTS
  DNA sequencing confirmed that the isolated obesity gene was identical to the previously reported sequence. The recombinant plasmid pBV220-OB was constructed and leptin successfully expressed in E.coli.
  
  
  CONCLUSIONS
  Successful cloning and expression of human obesity gene in E.coli may facilitate further research of the mechanism of fat metabolism and adipocyte differentiation.
authors.affiliation: Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhu
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Mao
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D020738:Leptin