Interference of laccase in determination of cellobiose dehydrogenase activity of Pleurotus ostreatus (Florida) using dichlorophenol indophenol as the electron acceptor.
Pleurotus ostreatus (Florida), ITCC 3308 produces approximately 9.0 U/ml extracellular cellobiose dehydrogenase (CDH) in cellulose medium after 7 days of growth. However, no activity could be detected if the assay was done with cellobiose as the substrate and 2,6-dichlorophenol indophenol (DPIP) as the electron acceptor in absence of any laccase inhibitor. Kinetic study showed that V(max)/K(m) value was very high for rDPIP (reduced 2,6-dichlrophenol indophenol) oxidation by laccase. Oxygen consumption rate of rDPIP oxidation by the enzyme was found to be highest among all the tested substrates. The present study indicated that rDPIP was a good substrate for laccase. Therefore, caution is needed to measure CDH activity by monitoring DPIP reduction in a system where laccase is likely to be present.
Saha
Tanima
T
Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Calcutta 700 032, India.
Chakraborty
Tapas Kumar
TK
Saha
Rina
R
Das
Nirmalendu
N
Mukherjee
Mina
M
D015086:2,6-Dichloroindophenol; D002237:Carbohydrate Dehydrogenases / Q000032:analysis* / Q000378:metabolism; D002482:Cellulose; D003470:Culture Media; D007700:Kinetics; D042845:Laccase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism; D020076:Pleurotus / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
D003470:Culture Media; D002482:Cellulose; D015086:2,6-Dichloroindophenol; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D042845:Laccase