Comparative in vitro evaluation of BL-P1654 and carbenicillin against Pseudomonas.
Prior investigators have reported a broad gram-negative spectrum and a marked anti-Pseudomonas activity in vitro with BL-P1654, 6-[R-alpha-(guanylureido)phenylacetamido]-penicillanic acid. An in vitro comparison of BL-P1654 to carbenicillin was performed against 84 strains of Pseudomonas and 39 strains of Enterobacteriaceae. Mean minimal inhibitory concentrations in Mueller-Hinton broth of BL-P1654 and carbenicillin were 12.5 and 100 mug/ml, respectively. Bactericidal studies with representative strains of Pseudomonas showed a consistent lack of bactericidal activity with BL-P1654. Mean bactericidal concentrations of BL-P1654 and carbenicillin were >/=200 and 100 mug/ml, respectively. The lack of bactericidal activity of BL-P1654 was further substantiated with the "killing curve" technique. Based on these data, BL-P1654 can not be considered a substitute for carbenicillin as the penicillin analogue of choice for Pseudomonas infections.
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D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002228:Carbenicillin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects*
D000900:Anti-Bacterial Agents; C009705:BLP 1654; D010397:Penicillanic Acid; D002228:Carbenicillin