title: The structure of the bacteriophage PRD1 spike sheds light on the evolution of viral capsid architecture.
abstract: Comparisons of bacteriophage PRD1 and adenovirus protein structures and virion architectures have been instrumental in unraveling an evolutionary relationship and have led to a proposal of a phylogeny-based virus classification. The structure of the PRD1 spike protein P5 provides further insight into the evolution of viral proteins. The crystallized P5 fragment comprises two structural domains: a globular knob and a fibrous shaft. The head folds into a ten-stranded jelly roll beta barrel, which is structurally related to the tumor necrosis factor (TNF) and the PRD1 coat protein domains. The shaft domain is a structural counterpart to the adenovirus spike shaft. The structural relationships between PRD1, TNF, and adenovirus proteins suggest that the vertex proteins may have originated from an ancestral TNF-like jelly roll coat protein via a combination of gene duplication and deletion.
authors.affiliation: Programme on Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Viikinkaari 1 00014, Finland.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Merckel
authors.forename: Juha T
authors.initials: JT
authors.lastname: Huiskonen
authors.forename: Dennis H
authors.initials: DH
authors.lastname: Bamford
authors.forename: Adrian
authors.initials: A
authors.lastname: Goldman
authors.forename: Roman
authors.initials: R
authors.lastname: Tuma
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry; D000595:Amino Acid Sequence; D025622:Bacteriophage PRD1 / Q000737:chemistry*; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D019143:Evolution, Molecular; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013059:Spectrum Analysis, Raman; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins