[Quantitative analysis of the nucleosides in Cordyceps sinensis with capillary zone electrophoresis].
OBJECTIVE
To establish a quantitative analysis method for analyzing the nucleosides in Cordyceps sinensis with capillary electrophoresis, and compare the difference between natural and the cultured C. mycelia.


METHODS
Capillary zone electrophoresis method was employed to quantitate the adenosine, uridine, guanosine and inosine in C. sinensis, with 0.25 mg x L(-1) boric acid-sodium hydroxide buffer, pH 9.5. The working voltage was 20 kV, the temperature was 25 degrees C, and the detection wavelength was 260 nm.


RESULTS
With the capillary zone electrophoresis method, the average recovery of the above 4 nucleosides was 98.9%, 95.1%, 97.8% and 98.8% respectively, with the RSD 0.4%, 1.7%, 1.3% and 5.0%. There was no adenosine in natural C. sinensis and no inosine in the cultured C. mycelia detected.


CONCLUSIONS
This method can be used to determine the adenosine, uridine, guanosine and inosine in C. sinensis. The nucleosides in C. sinensis produced from Qinghai province and cultured C. mycelia are obviously different.
Hou
Xiao-Rong
XR
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310027, China.
Luan
Lian-Jun
LJ
Cheng
Yi-Yu
YY
D000241:Adenosine / Q000032:analysis*; D000818:Animals; D032661:Cordyceps / Q000737:chemistry* / Q000145:classification; D046508:Culture Techniques; D019075:Electrophoresis, Capillary / Q000379:methods; D006151:Guanosine / Q000032:analysis; D007288:Inosine / Q000032:analysis*; D007915:Lepidoptera / Q000737:chemistry; D014529:Uridine / Q000032:analysis
D006151:Guanosine; D007288:Inosine; D000241:Adenosine; D014529:Uridine