[Establishment of a model of retinal microglial cells activation in vitro].
OBJECTIVE
To develop a method of retinal microglial cell culture to study the function of the microglial cell in diabetic retinopathy.


METHODS
Microglia were activated with LPS. Immunocytochemistry, con-focal microscopy, flow cytometry, MTT and ELISA were applied to observe the morphological characters, quantity, and functional changes of the microglia.


RESULTS
The purity of the microglia was up to 96% as determined by immunostaining and flow cytometry. Some morphological changes of microglia were observed after treatment with LPS, but their quantity kept stable. Cytokine TNF-alpha released from microglia increased significantly.


CONCLUSIONS
The isolated microglial cells are pure by using this culture system, which would provide a valuable tool for studying mechanisms of microglial alterations in diabetic retinopathy.
Wang
Ai-ling
AL
Peking University Third Hospital, Peking University Eye Center, Beijing 100083, China. alwang@bjmu.edu.cn
Liu
Lin
L
Zhu
Xiu-an
XA
Cao
An-min
AM
D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000166:cytology* / Q000378:metabolism; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha