Role of protein synthesis in the protection conferred by ozone-oxidative-preconditioning in hepatic ischaemia/reperfusion.
The liver is damaged by sustained ischaemia during liver transplantation, and the reperfusion after ischaemia results in further functional impairment. Ozone oxidative preconditioning (OzoneOP) protected the liver against ischaemia/reperfusion (I/R) injury through different mechanisms. The aim of this study was to investigate the influence of the inhibition of protein synthesis on the protective actions conferred by OzoneOP in hepatic I/R. Rats were treated with cycloheximide (CHX) in order to promote protein synthesis inhibition after OzoneOP treatment. Plasma transaminases, malondialdehyde and 4-hydroxyalkenals and morphological characteristics were measured as an index of hepatocellular damage; Cu/Zn-superoxide dismutase (SOD), Mn-SOD, catalase, total hydroperoxides and glutathione levels as markers of endogenous antioxidant system. OzoneOP increased Mn-SOD isoform and ameliorated mitochondrial damage. CHX abrogated the protection conferred by OzonoOP and decreased Mn-SOD activity. Cellular redox balance disappeared when CHX was introduced. Protein synthesis is involved in the protective mechanisms mediated by OzoneOP. Ozone treatment preserved mitochondrial functions and cellular redox balance.
Ajamieh
Hussam H
HH
Center for Research and Biological Evaluation (CIEB-IFAL), University of Havana, Havana, Cuba.
Berlanga
Jorge
J
Merino
Nelson
N
Sánchez
Gregorio M
GM
Carmona
Anna M
AM
Cepero
Silvia M
SM
Giuliani
Atilia
A
Re
Lamberto
L
León
Olga S
OS
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D019194:Ischemic Preconditioning; D008099:Liver / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism* / Q000648:ultrastructure; D016031:Liver Transplantation / Q000009:adverse effects / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010126:Ozone / Q000008:administration & dosage*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D011500:Protein Synthesis Inhibitors; D010126:Ozone; D003513:Cycloheximide; D002374:Catalase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D019803:Glutathione Disulfide