Identifying Plasmodium falciparum merozoite surface protein-10 human erythrocyte specific binding regions.
Receptor-ligand interactions between synthetic peptides and normal human erythrocytes were studied to determine P. falciparum merozoite surface protein-10 (MSP-10) regions specifically binding to membrane surface receptors on human erythrocytes. Three MSP-10 protein High Activity Binding Peptides (HABPs) were identified, whose binding to erythrocytes became saturable and sensitive on being treated with neuraminidase, trypsin and chymotrypsin. Some of them specifically recognised a 50 kDa erythrocyte membrane protein. Some HABPs inhibited in vitro P. falciparum merozoite invasion of erythrocytes by 70%, suggesting that MSP-10 protein's possible role in the invasion process probably functions by using similar mechanisms to those described for other MSP family antigens. In addition to above results, the high homology in amino-acid sequence and superimposition of both MSP-10, MSP-8 and MSP-1 EGF-like domains and HABPs 31132, 26373 and 5501 suggest that tridimensional structure could be playing an important role in the invasion process and in designing synthetic multi-stage anti-malarial vaccines.
Puentes
Alvaro
A
Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Bogotá, Colombia. puentesalvaro@hotmail.com
Ocampo
Marisol
M
Rodríguez
Luis Eduardo
LE
Vera
Ricardo
R
Valbuena
John
J
Curtidor
Hernando
H
García
Javier
J
López
Ramsés
R
Tovar
Diana
D
Cortes
Jimena
J
Rivera
Zuly
Z
Patarroyo
Manuel Elkin
ME
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002918:Chymotrypsin / Q000494:pharmacology; D004910:Erythrocyte Membrane; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D006801:Humans; D016778:Malaria, Falciparum / Q000378:metabolism; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000378:metabolism / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000378:metabolism / Q000469:parasitology*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D010446:Peptide Fragments; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D009439:Neuraminidase; D002918:Chymotrypsin; D014357:Trypsin