title: Monitoring processed, mature Human Immunodeficiency Virus type 1 particles immediately following treatment with a protease inhibitor-containing treatment regimen.
abstract: Protease inhibitors (PIs) block HIV-1 maturation into an infectious virus particle by inhibiting the protease processing of gag and gag-pol precursor proteins. We have used a simple anti-HIV-1 p24 Western blot to monitor the processing of p55gag precursor into the mature p24 capsid immediately following the first dosage of a PI-containing treatment regimen. Evidence of PI activity was observed in plasma virus as early as 72 hours post treatment-initiation and was predictive of plasma viral RNA decrease at 4 weeks.
authors.affiliation: Division of Infectious Disease, Department of Medicine, Case School of Medicine and the Center for AIDS Research, Case Western Reserve University, University Hospitals of Cleveland, Cleveland, OH, USA. eja3@case.edu.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Baird
authors.forename: Andre J
authors.initials: AJ
authors.lastname: Marozsan
authors.forename: Michael M
authors.initials: MM
authors.lastname: Lederman
authors.forename: Alan
authors.initials: A
authors.lastname: Landay
authors.forename: Donna
authors.initials: D
authors.lastname: Mildvan
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Kuritzkes
authors.forename: Harold A
authors.initials: HA
authors.lastname: Kessler
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Arts