title: Viral RNA is required for the association of APOBEC3G with human immunodeficiency virus type 1 nucleoprotein complexes.
abstract: APOBEC3G (APO3G) is a host cytidine deaminase that is incorporated into human immunodeficiency virus type 1 (HIV-1) particles. We report here that viral RNA promotes stable association of APO3G with HIV-1 nucleoprotein complexes (NPC). A target sequence located within the 5'-untranslated region of the HIV-1 RNA was identified to be necessary and sufficient for efficient APO3G packaging. Fine mapping revealed a sequence normally involved in viral genomic RNA dimerization and Gag binding to be important for APO3G packaging and association with viral NPC. Our data suggest that packaging of APO3G into HIV-1 NPC is enhanced by viral RNA.
authors.affiliation: NIH, NIAID, 4/312, 4 Center Drive, MSC 0460, Bethesda, MD 20892-0460, USA.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Khan
authors.forename: Sandra
authors.initials: S
authors.lastname: Kao
authors.forename: Eri
authors.initials: E
authors.lastname: Miyagi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Ritu
authors.initials: R
authors.lastname: Goila-Gaur
authors.forename: Sandrine
authors.initials: S
authors.lastname: Opi
authors.forename: Clay L
authors.initials: CL
authors.lastname: Gipson
authors.forename: Tristram G
authors.initials: TG
authors.lastname: Parslow
authors.forename: Hinh
authors.initials: H
authors.lastname: Ly
authors.forename: Klaus
authors.initials: K
authors.lastname: Strebel
mesh_terms: D020121:5' Untranslated Regions / Q000378:metabolism; D000071480:APOBEC-3G Deaminase; D003564:Cytidine Deaminase; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009698:Nucleoproteins / Q000378:metabolism*; D009700:Nucleoside Deaminases; D011506:Proteins / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D012097:Repressor Proteins; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D009698:Nucleoproteins; D011506:Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; D009700:Nucleoside Deaminases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase