[The impact of different provocations on ERK translocation and c-fos expression in neonatal cultured cardiomyocytes].
OBJECTIVE
To study the nuclear translocation of extracellular signal regulated kinase (ERK) and expression of c-fos mRNA under stimulation of AngII as well as the influence on the nuclear translocation of ERK with different interferences in neonatal cultured cardiomyocytes.


METHODS
ERK in the cytoplasm or nucleus was observed by immunocytochemistry using specific antibody and the expression of c-fos was evaluated with RT-PCR technique.


RESULTS
AngII (10(-6) mol/L) had the effect in promoting activation of ERK and then appeared in nucleus rapidly. The translocation process of ERK induced by AngII was blocked distinctly by Valsartan (10(-5) mol/L) and PD98059 (5 x 10(-5) mol/L), but not by CGP42112A (10(-5) mol/L). It was also found that Valsartan and PD98059 could inhibit the expression of c-fos in that process.


CONCLUSIONS
The nuclear translocation of ERK might be a precondition for the inducement of c-fos expression.
Li
Ai-ping
AP
Cardiovascular Center of Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing 100730, China.
Chen
Guang-hui
GH
Li
Tian-de
TD
Wang
You-gui
YG
Li
Zhi-min
ZM
Hu
Da-yi
DY
D021581:Active Transport, Cell Nucleus; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000804:Angiotensin II; D020928:Mitogen-Activated Protein Kinases