title: Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells.
abstract: EFA6C is a third member of the EFA6 family of guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factor 6 (ARF6). In this study, we first demonstrated that EFA6C indeed activated ARF6 more selectively than ARF1 by ARF pull-down assay. In situ hybridization histochemistry revealed that EFA6C mRNA was expressed predominantly in mature Purkinje cells and the epithelial cells of the choroid plexus in contrast to the ubiquitous expression of ARF6 mRNA throughout the brain. EFA6C mRNA was already detectable in the Purkinje cells at embryonic day 13, increased progressively during post-natal development and peaked during post-natal second week. In Purkinje cells, the immunoreactivity for EFA6C was localized particularly in the post-synaptic density as well as the plasma membranes of the cell somata, dendritic shafts and spines, while the immunoreactivity in their axon terminals in the deep cerebellar nuclei was very faint. These findings suggest that EFA6C may be involved in the regulation of the membrane dynamics of the somatodendritic compartments of Purkinje cells through the activation of ARF6.
authors.affiliation: Division of Histology, Department of Cell Biology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shigetsune
authors.initials: S
authors.lastname: Matsuya
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Akira
authors.initials: A
authors.lastname: Tohgo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Shin
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeshima
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakayama
authors.forename: Shoichi
authors.initials: S
authors.lastname: Kokubun
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis / Q000378:metabolism*; D006367:HeLa Cells; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000378:metabolism*; D011689:Purkinje Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
chemical_list: D000089982:ADP-Ribosylation Factor 6; D020662:Guanine Nucleotide Exchange Factors; D009419:Nerve Tissue Proteins; C109970:PSD protein, human; D010445:Peptide Elongation Factors; C504474:Psd protein, mouse; C000715348:ARF6 protein, human; C000715727:Arf6 protein, mouse