Genistein reverses changes of the proteome induced by oxidized-LDL in EA.hy 926 human endothelial cells.
Endothelial cells are primary targets for pro-atherosclerotic stressors such as oxidized LDL (ox-LDL). The isoflavone genistein, on the other hand, is suggested to prevent a variety of processes underlying atherosclerosis and cardiovascular diseases. By analyzing the proteome of EA.hy 926 endothelial cells, here we show, that genistein reverses the ox-LDL-induced changes of the steady-state levels of several proteins involved in atherosclerosis. These alterations caused by genistein are functionally linked to the inhibition of ox-LDL induced apoptosis.
Fuchs
Dagmar
D
Department of Food and Nutrition, Molecular Nutrition Unit, Technical University of Munich, Hochfeldweg 2, D-85350 Freising, Germany.
Erhard
Petra
P
Turner
Rufus
R
Rimbach
Gerald
G
Daniel
Hannelore
H
Wenzel
Uwe
U
D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D010449:Peptide Mapping; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000737:chemistry
D008077:Lipoproteins, LDL; D020543:Proteome; C099252:oxidized low density lipoprotein; D019833:Genistein; D014357:Trypsin