title: Computer image analysis of ultrasound biomicroscopy of primate accommodation.
abstract: OBJECTIVE
  To assess and correct images of the eye for movements that can confound the evaluation of the presence, direction, and magnitude of intraocular movement of the crystalline lens equator during centrally induced ciliary muscle contraction (accommodation).
  
  
  METHODS
  Ultrasound biomicroscopic (UBM) video images of a cynomologus monkey crystalline lens were obtained from an independent source. The images, prior to, during, and following electrical stimulation of the Edinger-Westphal (EW) nucleus were compared for evidence of movement of the crystalline lens equator. Extraocular eye movements were assessed by use of objective computer imaging analysis techniques.
  
  
  RESULTS
  Extraocular eye movements were identified and reduced by using objective computer imaging analysis techniques to register and realign the corneal images. Highly significant corrections are required to effect corneal realignment. Analysis of paired and registered images from this data source indicates that any movements of the primate lens equator are not detectable when maximum accommodation was induced by EW stimulation.
  
  
  CONCLUSIONS
  The displacement of the edge of the primate crystalline lens equator during electrically induced contraction of the ciliary muscle is a small displacement phenomenon, only analysable after confounding extraocular movements are removed from the compared images.
authors.affiliation: Department of Physics, University of Texas at Arlington, Arlington, TX, USA. ron@2ras.com
authors.forename: R A
authors.initials: RA
authors.lastname: Schachar
authors.forename: F
authors.initials: F
authors.lastname: Kamangar
mesh_terms: D000065:Accommodation, Ocular; D000818:Animals; D016477:Artifacts; D002924:Ciliary Body / Q000502:physiology; D003315:Cornea / Q000502:physiology; D004558:Electric Stimulation / Q000379:methods; D005133:Eye Movements / Q000502:physiology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007908:Lens, Crystalline / Q000502:physiology*; D008252:Macaca fascicularis / Q000502:physiology*; D033401:Microscopy, Acoustic / Q000379:methods; D009119:Muscle Contraction / Q000502:physiology