Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup.
Multiply primed rolling-circle amplification is a novel technology that uses bacteriophage phi29 DNA polymerase to amplify circular DNA molecules, without the need for prior knowledge of their sequences. In an attempt to detect Torque teno virus (TTV), rolling-circle amplification was used to amplify DNA extracted from eight human and four pig serum samples. All samples gave high molecular weight (>30 kb) amplification products. By restriction endonuclease digestion, these products generated DNA fragments whose sizes were consistent with those of human TTV (3.8 kb) and swine TTV (Sd-TTV; 2.9 kb) genomes. Two TTV isolates derived from a single AIDS patient, as well as two Sd-TTV isolates derived from a single pig, were characterized by complete nucleotide sequencing. One of the Sd-TTV isolates showed very low (43-45 %) nucleotide sequence similarity to the other Sd-TTV isolate and to the prototype isolate Sd-TTV31, and could be considered the prototype of a novel genogroup.
Niel
Christian
C
Department of Virology, Oswaldo Cruz Institute, Avenida Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
Diniz-Mendes
Leonardo
L
Department of Virology, Oswaldo Cruz Institute, Avenida Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
Devalle
Sylvie
S
Department of Virology, Oswaldo Cruz Institute, Avenida Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
D000328:Adult; D000818:Animals; D018173:Circoviridae Infections / Q000821:virology*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology; D022783:Torque teno virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D004279:DNA, Viral; D004262:DNA Restriction Enzymes