Effect of leptin on proliferative activity and vascular endothelial growth factor (VEGF) secretion from cultured endothelial cells HECa10 in vitro.
OBJECTIVE
The aim of this study was to check if leptin influences the proliferative activity and vascular endothelial grofth factor (VEGF) release from cultured mouse endothelial cells in vitro.


METHODS
The murine cell line HECa10 obtained from endothelial cells of mouse peripheral lymph nodes immortalised by transfection of plasmid with the gene for the large T antigen of Simian virus 40 was used in the experiments. The proliferative activity of HECa10 cells was studied by Mosmann and VEGF release by ELISA methods.


RESULTS
Murine leptin in concentrations from 5 to 25 ng/ml stimulated the proliferative activity of 72 h endothelial cell cultures and in concentrations from 0.5 to 25 ng/ml augmented the release of VEGF into supernatants of 24 h and 72 h cultured cells.


CONCLUSIONS
Leptin stimulated proliferation and VEGF secretion of endothelial cells in vitro.
Misztal-Dethloff
Beata
B
Institute of Endocrinology, Medical University of Lodz, 91-425 Lodz, Poland.
Stepien
Henryk
H
Komorowski
Jan
J
D000818:Animals; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D020738:Leptin / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D009994:Osmolar Concentration; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D020738:Leptin; D042461:Vascular Endothelial Growth Factor A