[Effect of the medicinal leech salivary gland secretion on the state of rat subcutaneous mast cells].
It is firstly showed that the medicinal leech salivary gland secretion (SGS) as a polycomponent system of proteins and low-molecular weight substances, activates rat subcutaneous mast cells in vitro prompting a decrease in the heparin saturation index and increasing some characteristic mast cells morphometric parameters. The same mast cell changes were detected by analysis of some specimens of subcutaneous cellular tissue in the point of skin injured by the leech bite. It is shown that these changes are saved during 3 days. The mechanical injury of rat skin does not effect the mast cells activation. Activation of mast cells by SGS is extended to the distant subcutaneous mast cells. It is expressed in sharp decreasing of heparin saturation index although not statistically positive. The secondary leeching on these distant points provokes reduction of mast cells activation and some decrease of post-leeching blood heparin content: 0.154 +/- 0.03 units/ml (n = 10) as compared with post-leeching blood heparin contents analysed from the wound after the primary leeching (0.160 +/- 0.03 units/ml, n = 10). Proceeding from these findings, participation of heparin secreted from activated mast cells in the support of post-leeching bleeding is suggested, the phenomenon which provides unloading of capillary pool by application of medicinal leeches for treatment many diseases.
Baskova
I P
IP
Gol'tsova
K V
KV
Zavalova
L L
LL
Klimovich
L G
LG
D000818:Animals; D001733:Bites and Stings; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology; D015550:Cell Degranulation / Q000187:drug effects*; D006493:Heparin / Q000097:blood / Q000378:metabolism*; D047709:Hirudo medicinalis / Q000378:metabolism*; D024763:Leeching; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D051381:Rats; D012469:Salivary Glands / Q000378:metabolism*; D012867:Skin / Q000098:blood supply
D006493:Heparin