Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR).
The prevalence of hepatopancreatic parvovirus (HPV) in wild penaeid shrimp samples from India was studied by nested polymerase chain reaction (PCR) using primers designed in our laboratory. The virus could be detected in 9 out of 119 samples by non-nested PCR. However, by nested PCR 69 out of 119 samples were positive. The PCR results were confirmed by hybridization with digoxigenin-labelled DNA probe. Shrimp species positive by non-nested PCR included Penaeus monodon, Penaeus indicus and Penaeus semisulcatus and by nested PCR Parapenaeopsis stylifera, Penaeus japonicus, Metapenaeus monoceros, M. affinis, M. elegans, M. dobsoni, M. ensis and Solenocera choprai. This is the first report on the prevalence of HPV in captured wild shrimp from India.
Manjanaik
B
B
Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India.
Umesha
K R
KR
Karunasagar
Indrani
I
Karunasagar
Iddya
I
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015342:DNA Probes; D004076:Digoxigenin; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017992:Parvovirus / Q000235:genetics*; D033561:Penaeidae / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D004076:Digoxigenin