title: [High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium].
abstract: OBJECTIVE
  To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC).
  
  
  METHODS
  ODS HYPERSIL C(18) column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used.
  
  
  RESULTS
  The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively; relative standard deviations (RSDs) of within-day and between-days were less than 1.5% and 5.1%, respectively.
  
  
  CONCLUSIONS
  The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium.
authors.affiliation: Department of Cardiology, Xi yuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. miaoyu0609@sina.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Miao
authors.forename: Cheng-long
authors.initials: CL
authors.lastname: Wang
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Yin
authors.forename: Da-zhuo
authors.initials: DZ
authors.lastname: Shi
authors.forename: Ke-ji
authors.initials: KJ
authors.lastname: Chen
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate