[Inhibition of binding peptides on replication of duck hepatitis B virus].
To study the inhibitory effect of binding peptides on duck hepatitis B virus (DHBV) replication in duck hepatocytes.



Specific binding peptides to duck hepatitis B virus polymerase (DHBVP) were screened by phage display technology (PDT), then were sequenced and synthesized. Binding peptides were added into primary culture of duck hepatocytes infected with DHBV in vitro. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.



Seven binding peptides were obtained after 3-round screening by PDT. Duck primary hepatocytes infected by DHBV were treated with above obtained binding peptides. The DHBV-DNA levels in medium supernatant and cytoplasm of duck hepatocytes treated with synthesized peptides (the 3rd and the 6th peptide) were significantly lower than those of control cells (P<0.05).



Specific binding peptides to DHBVP could inhibit the replication of DHBV.
Jia
Hong-yu
HY
Institute of Infectious Disease, The First Affiliated Hospital, Key Laboratory of Infectious Disease Ministry of Health, College of Medicine, Zhejiang University, Hangzhou 310003, China. jia-hy@tom.com
Chen
Zhi
Z
Zhou
Lin-fu
LF
Chen
Feng
F
Zhu
Hai-hong
HH
Liu
Ji-hong
JH
Xu
Xiao-yan
XY
D000818:Animals; D002478:Cells, Cultured; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000821:virology*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000235:genetics; D006524:Hepatitis, Viral, Animal / Q000821:virology; D022781:Hepatocytes / Q000821:virology; D020135:Peptide Nucleic Acids / Q000494:pharmacology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D020135:Peptide Nucleic Acids; D012194:RNA-Directed DNA Polymerase