T11TS/S-LFA3 induces apoptosis of the brain tumor cells: a new approach to characterise the apoptosis associated genetic changes by arbitrarily primed-PCR.
Genetic alterations in ethyl nitrosourea (ENU) induced brain tumor model were analysed by simple PCR based technique with arbitrary primers. T11TS/SLFA3 was established previously as a potent immune stimulator with antineoplastic property in experimental glioma model. The goal of this study was to reveal whether T11TS induces apoptosis of the neural neoplastic cell and to decipher the DNA polymorphism level of the cells undergoing apoptosis. The results clearly establish the apoptogenic role of T11TS/S-LFA3 and along with the detection of cancer associated genomic instability, AP-PCR analysis is useful for the detection of DNA level fragmentation, a unique feature of apoptosis.
Mukherjee
Joydeep
J
Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Ghosh
Anirban
A
Sarkar
Susobhan
S
Mazumdar
Malabika
M
Sarkar
Pallab
P
Duttagupta
Ashish Kumar
AK
Chaudhuri
Swapna
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology*; D018801:CD2 Antigens / Q000494:pharmacology*; D018968:CD58 Antigens / Q000494:pharmacology*; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis*; D004195:Disease Models, Animal; D005038:Ethylnitrosourea; D005260:Female; D020287:In Situ Nick-End Labeling; D008297:Male; D010718:Phosphatidylserines / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D018801:CD2 Antigens; D018968:CD58 Antigens; D004273:DNA, Neoplasm; D010718:Phosphatidylserines; D005038:Ethylnitrosourea