A simple method of DNA extraction in solving difficult criminal cases.
OBJECTIVE
We examined combined use in house method and DNA IQ system (Promega) procedure for the extraction DNA from biological trace in order to help the solving some crime cases.


METHODS
Simple and efficient method for extraction of the DNA from biological trace samples included the well washed trousers and the knife without any visual biological trails (from some one hard criminal case). In this method there were used: centrifuge, microwave oven, shaker, chelex, proteinase K, DNA IQ System (Promega).


RESULTS
The extraction of DNA could be done within 2.5 hours or over the night. The DNA prepared on this way was good quality and could be used for STR analysis.


CONCLUSIONS
This combined, simple, and safe method could be used for extraction DNA from samples containing a minute amount of the biological trace.
Eminovic
Izet
I
Department for molecular pathology Tuzla, Policlinic for microbiology pathology immunology and molecular medicine, University Clinical Centre of Tuzla, Bosnia and Herzegovina.
Karamehić
Jasenko
J
Gavrankapetanović
Faris
F
Heljić
Bećir
B
D004247:DNA / Q000302:isolation & purification*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction
D004247:DNA