title: Use of ligase chain reaction in early diagnosis of tuberculous meningitis.
abstract: BACKGROUND
  Nucleic acid-based amplification tests are currently licensed only for the detection of Mycobacterium tuberculosis in pulmonary specimens. There are insufficient data for extrapulmonary specimens. The aim of this study is to investigate the diagnostic value of these investigations in tuberculous meningitis.
  
  
  METHODS
  We performed a prospective study using a commercial ligase chain reaction DNA amplification technique [Ligase chain result (LCx) M. tuberculosis; Abbott Laboratories, Abbott Park, IL, USA] on cerebrospinal fluid (CSF) to diagnose tuberculous meningitis and compared the results with standard microbiological data. Conflicting cases were resolved according to the final clinical diagnosis. A total of 54 CSF specimens from 54 patients were tested.
  
  
  RESULTS
  Six (11.1%) specimens were culture-positive for M. tuberculosis; of these, only 1 (1.9%) was smear-positive. The sensitivity, specificity, positive predictive value and negative predictive value of the LCx assay, compared with culture results, were 66.7%, 100%, 100% and 96%, respectively. After resolution according to clinical data, the sensitivity, specificity, positive predictive value and negative predictive value were 33.3%, 100%, 100% and 75%, respectively. All controls had negative LCx results. There were no false positives.
  
  
  CONCLUSIONS
  LCx assay is highly specific and complements conventional laboratory diagnostic methods (CSF smear and culture) in the diagnosis of tuberculous meningitis. In the appropriate clinical context, a positive result is strongly suggestive of tuberculosis and could enable antituberculous treatment to be started immediately.
authors.affiliation: National Neuroscience Institute (Tan Tock Seng Hospital), Singapore. hoe_chin_chua@nni.com.sg
authors.forename: H C
authors.initials: HC
authors.lastname: Chua
authors.forename: L
authors.initials: L
authors.lastname: Tay
authors.forename: S X
authors.initials: SX
authors.lastname: Wang
authors.forename: Y C
authors.initials: YC
authors.lastname: Chan
mesh_terms: D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid / Q000175:diagnosis*