title: Real-time reporting of circadian-regulated gene expression by luciferase imaging in plants and mammalian cells.
abstract: Luciferase enzymes have been used as reporters of circadian rhythms in organisms as diverse as cyanobacteria, plants, fruit flies, and mice. This article details methodology for real-time reporting of circadian-regulated gene expression by imaging of luciferase bioluminescence in plants and mammalian cells.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: David K
authors.initials: DK
authors.lastname: Welsh
authors.forename: Takato
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Steve A
authors.initials: SA
authors.lastname: Kay
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D002940:Circadian Rhythm / Q000502:physiology*; D005583:Fourier Analysis; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D051379:Mice; D008822:Mice, Transgenic; D030821:Plants, Genetically Modified / Q000201:enzymology
chemical_list: D008156:Luciferases