The intraluminal thread model revisited: rat strain differences in local cerebral blood flow.
There are major differences in the outcome of focal cerebral ischemia between rat strains. This study aimed to investigate whether inter-strain differences exist in the local cerebral blood flow, changes produced by intraluminal middle cerebral artery occlusion (MCAO). Fifty-four male Long-Evans, Sprague-Dawley or Wistar rats were subjected to 60 minutes of transient MCAO, carried out with a silicone-coated 4-0 nylon monofilament. Local cerebral blood flow was monitored on the parietal cortex with laser-Doppler flowmetry. The decrease in local cerebral blood flow during the first 20 minutes of occlusion was significantly greater in Long-Evans (74.6 +/- 8.9%) than in Sprague-Dawley (50.7 +/- 26.8%) and Wistar (49.0 +/- 21.9%) rats. Strain differences in local cerebral blood flow reduction were maintained, or increased, throughout the occlusion period. These results show that the intraluminal model of MCAO produces a more pronounced, persistent and stable local cerebral blood flow reduction, with less interanimal variability, in Long-Evans than in Sprague-Dawley or Wistar rats.
Prieto
Ruth
R
Service of Neurosurgery, University Hospital La Paz, Madrid, Spain. rprieto.hulp@salud.madrid.org
Carceller
Fernando
F
Roda
José María
JM
Avendaño
Carlos
C
D000704:Analysis of Variance; D000818:Animals; D001776:Blood Circulation Time / Q000379:methods; D002545:Brain Ischemia / Q000503:physiopathology*; D002244:Carbon; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry / Q000379:methods; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity; D013997:Time Factors
C028433:chinese ink; D002244:Carbon