[The expression of VEGF and iNOS in laryngeal squamous cell carcinoma and its clinical significance].
OBJECTIVE
To investigate the mRNA expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in laryngeal squamous carcinoma and its correlation with clinicopathologic parameters, to identify the interactions between the expression of VEGF and iNOS.


METHODS
The mRNA expression of VEGF and iNOS in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 40 patients were examined by reverse transcription-polymerase chain reaction.


RESULTS
The positive expression rate of VEGF and iNOS in tumor samples were significantly higher than that in normal tissues (P < 0.01); in tumor samples, their expression were not associated with age, sex, tumor site and T stage (P > 0.05); their expression were higher in moderately and poorly differentiated tumors than in well differentiated tumors (P < 0.05); their expression were significantly higher in tumors with cervical lymph node metastasis than in tumor without cervical lymph node metastasis (P < 0.01). There were correlation between the expression of VEGF and iNOS in tumor samples (P < 0.05).


CONCLUSIONS
VEGF and iNOS could up-regulate each other to promote the development and cervical lymph node metastasis of laryngeal squamous carcinoma.
Sun
Na
N
Department of Otolaryngology, Shanghai Gongli Hospital, Shanghai, 200135, China.
Ge
Rongming
R
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D052247:Nitric Oxide Synthase Type II