Serum protein media are important factors in the manual hexadimethrine bromide (polybrene) test, experience in China.
OBJECTIVE
The use of the manual hexadimethrine bromide (polybrene) test in routine cross-matching after accurately detecting cell grouping and irregular antibodies is prevalent in China. This article reports the importance of serum protein mediums in the performance of the manual hexadimethrine bromide test.


METHODS
Blood group O red blood cells and Blood group AB and Rh positive serum were collected at random from healthy blood donators, IgG anti-D serum separated from pregnant woman, then tested with each other by the manual hexadimethrine bromide methods in routine tests and some designed corresponding tests with IgG, IgM anti-D monoclonal diagnostic reagents and some serum protein components.


RESULTS
Red blood cells that were adjusted to 3-5% suspension by normal saline then only added in 0.7 ml low ionic medium (LIM) and two drops of polybrene solution adhere to the surface of test tubes' bottom when centrifuged, so it was difficult to perform the next approach, but the adherence disappeared when red blood cells' concentrations exceeded 20-30%. Rh positive red blood cells coated by anti-D have the same phenomenon. This adherence can be prevented by serum medium diluted from 1:128 to 1:1024 times by normal saline and hemoglobin medium diluted from 1:32 to 1:128 times, but not by albumin or immunoglobulin medium. The denary logarithm values of the greatest inhibited dilutions of serum and hemoglobin elution between antibody sensitizing red blood cells and the same pre-sensitizing red blood cells tests were no significant difference (P value > 0.05).


CONCLUSIONS
The whole serum or serum protein mediums are important factors that can influence successfully performance of the manual hexadimethrine bromide test. So appliance of the manual hexadimethrine bromide test to immunohematology laboratory, such as when performing titrations of serum or plasma, or when testing eluates for antibody activity, this adherence must be considered.
Liu
Feng
F
Department of Blood Transfusion, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Avenue, 430022 Wuhan, Hubei Province, China. liu_feng_email@sina.com
Hu
Li-Hua
LH
D016477:Artifacts; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001798:Blood Proteins / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002498:Centrifugation / Q000295:instrumentation; D002681:China; D003298:Coombs Test / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004906:Erythrocyte Count; D004910:Erythrocyte Membrane / Q000276:immunology; D005898:Glass; D006583:Hexadimethrine Bromide / Q000494:pharmacology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D010969:Plastics; D011506:Proteins / Q000494:pharmacology*; D011933:Reagent Kits, Diagnostic; D018029:Rho(D) Immune Globulin
D001798:Blood Proteins; D003470:Culture Media; D016895:Culture Media, Serum-Free; D007518:Isoantibodies; D010969:Plastics; D011506:Proteins; C061961:RHO(D) antibody; D011933:Reagent Kits, Diagnostic; D018029:Rho(D) Immune Globulin; D006583:Hexadimethrine Bromide