[Effects of sevofluran on cell division and levels of sister chromatid exchange].
OBJECTIVE
Purpose of the study was to investigate the mitotic index (MI) and sister chromatid exchange (SCE) levels to identify the mutagenic and carcinogenic effects of sevoflurane (sevoflurane).


METHODS
42 non-smoking male and female turkish patients of ASA-risk I and II were included. The patients received an anaesthesia induction with 8 % sevoflurane in 100 % oxygen ("tidal volume methode") and 0.1 mg/kg BW vecuronium for neuromuscular block and endotracheal intubation. Anaesthesia was maintained with 2.0 - 2.5 sevoflurane in 60 % N(2)O and 40 % O(2). Four 5 ml venous blood samples werde taken: before induction (control), 60 minutes, 24 hours and 5 days after sevoflurane anesthesia. Samples were prepared according to the periferic blood culture assay, modified by Morhead and co-workers, and levels of MI and SCE were examined.


RESULTS
60 minutes after sevoflurane-anaesthesia a significant decrease of MI was found compared to controls (p < 0.01). This depression was lower after 24 hours (p < 0.05) and reversible after 5 days. SCE increased significantly during 60 minutes of anaesthesia (p < 0.001), was also lower after 24 hours (5.6 +/- 2.4 vs. 4.4 +/- 1.7) and returned to normal levels after 5 days (p > 0.05).


CONCLUSIONS
The application of sevoflurane for anaesthesia may influence the cell division in humans and may have a mutagenic effect on DNA at the cell level, which is reversible.
Lüleci
N
N
Celal Bayar-Universität, Medizinische Fakultät, Anästhesiologie Abteilung.
Sakarya
M
M
Topçu
I
I
Lüleci
E
E
Erinçler
T
T
Solak
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests; D000077149:Sevoflurane; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D004247:DNA