title: [Immunoglobulin isotype and IgG subclass profiles in American tegumentary leishmaniasis].
abstract: The present work investigated the serum antibody profiles in 37 patients with American tegumentary leishmaniasis, who were attended at Hospital de Clinicas - Universidade Federal de Uberlandia, MG, Brazil. The immunoglobulin class and IgG subclass profiles were analyzed by indirect ELISA using Leishmania (Leishmania) amazonensis soluble antigen. The antibody avidity was determined by 6 M urea treatment after incubation with immunoenzymatic conjugate. It was observed that 97% of the serum samples presented anti-Leishmania antibodies for IgE class, 94.6% IgG, 57.5% IgA and 21.5% IgM class. For IgG subclasses the profiles were in the following order of frequency: IgG1>IgG3>IgG2>IgG4. High avidity of anti-Leishmania IgE antibodies was found in 44.4% of the samples. On the other hand, moderate avidity of specific IgG and IgA was observed in 62.8% and 47.8% of samples, respectively. These results indicate a very complex antibody response profile against American tegumentary leishmaniasis.
authors.affiliation: Laboratório de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: de Souza
authors.forename: Adriano Gomes
authors.initials: AG
authors.lastname: da Silva
authors.forename: Sandra Regina
authors.initials: SR
authors.lastname: Afonso-Cardoso
authors.forename: Silvio Junior
authors.initials: SJ
authors.lastname: Favoreto
authors.forename: Marcelo Simão
authors.initials: MS
authors.lastname: Ferreira
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000915:Antibody Affinity; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology*; D007136:Immunoglobulins / Q000097:blood* / Q000276:immunology; D007892:Leishmania braziliensis / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D000913:Antibodies, Protozoan; D015415:Biomarkers; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins