title: West Nile virus in Canadian blood donors.
abstract: BACKGROUND
  Blood donation screening for West Nile virus (WNV) RNA by nucleic acid testing (NAT) was implemented in Canada in July 2003, and 14 WNV RNA-positive donations were identified. Samples were screened in minipools of six donations with a WNV assay (TaqScreen, Roche). Two of the donors were identified by single-donor screening that was initiated in the province of Saskatchewan, which had the highest prevalence of WNV in the country, in early September 2003.
  
  
  METHODS
  The original 14 samples and follow-up samples (2-35 days after donation), available from 13 of the 14 donors were tested with an in-house, real-time, quantitative WNV NAT assay that was specific for WNV. A Health Canada reference reagent was used for calibration. Immunoglobulin M (IgM) and immunoglobulin G (IgG) levels were determined with commercial enzyme-linked immunosorbent assay kits.
  
  
  RESULTS
  All donors tested positive for the presence of WNV with the in-house assay. Two donors, 18 and 19, identified by single-donor testing, had extremely low levels of viremia and that could only be detected in 1:38 or 1:39 replicate tests. The titers of the remaining index samples ranged from below log2.8 (the limit of quantitation) to log4.7 NAT detectable units per mL. Three samples, from Donors 17, 18, and 19, were IgM-positive, whereas samples from Donors 18 and 19 were also IgG-positive. The remaining 10 donors with follow-up samples all seroconverted.
  
  
  CONCLUSIONS
  The 14 WNV donor samples detected by routine screening were confirmed as WNV RNA-positive by a WNV RNA-specific in-house assay and by demonstration of seroconversion in 13 of the 14 donors.
authors.affiliation: Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: Cherie
authors.initials: C
authors.lastname: Cameron
authors.forename: Jackie
authors.initials: J
authors.lastname: Reeves
authors.forename: Nick
authors.initials: N
authors.lastname: Antonishyn
authors.forename: Peter
authors.initials: P
authors.lastname: Tilley
authors.forename: Ted
authors.initials: T
authors.lastname: Alport
authors.forename: Bernie
authors.initials: B
authors.lastname: Eurich
authors.forename: Dale
authors.initials: D
authors.lastname: Towns
authors.forename: Debra
authors.initials: D
authors.lastname: Lane
authors.forename: John
authors.initials: J
authors.lastname: Saldanha
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors; D002170:Canada / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008403:Mass Screening; D012367:RNA, Viral / Q000032:analysis; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014901:West Nile Fever / Q000097:blood* / Q000453:epidemiology* / Q000276:immunology; D014902:West Nile virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral