title: Time-dependent influence of supranutritional organically bound selenium on selenium accumulation in growing wether lambs.
abstract: Crossbred wethers (n = 36; BW = 36.0 kg; SD = 3.4) were used to assess the time-dependent influence of supranutritional organically bound Se on Se accumulation. Four wethers were slaughtered before the trial began (d 0). The remaining wethers were fed diets containing adequate (0.2 microg of Se/g of DM) or supranutritional Se (2.9 microg of Se/g of DM; in the form of high-Se wheat grain) for 14, 28, 42, or 56 d before slaughter (four wethers per Se treatment at each slaughter day). The DMI was set at 3.1% of BW and adjusted weekly based on a targeted ADG of 150 g. Daily Se intake by wethers fed the adequate and supra-nutritional Se diets ranged from 5.3 to 5.9, and 79.0 to 95.0 microg of Se/kg of BW, respectively, and did not differ (P = 0.84 to 0.99) between slaughter day groups within Se treatment. Neither Se treatment nor Se treatment x slaughter day interactions were significant for BW, G:F, or liver, kidneys, and spleen weights (P = 0.06 to 0.84). Within the supranutritional Se treatment, Se contents of most organs and tissues from wethers slaughtered on d 14, 28, 42, and 56 were nearly twice the concentrations (P < 0.01) of wethers slaughtered on d 0. When regressed against the number of days the wethers were fed supranutritional Se, Se concentrations increased (P < 0.001) cubically in kidneys and plasma, quadratically in duodenum, lung, liver, and spleen, and linearly in heart, muscle, and wool. For total Se in kidneys, liver, and spleen, the response was quadratic (P < 0.03). Excluding skeletal muscle, heart, and wool, Se in other organs and tissues reached apparent steady-state concentrations 14 to 28 d after commencement of supranutritional Se diets. Selenium concentrations in skeletal muscle accumulated in a linear manner (P < 0.001) throughout the 56-d feeding period. High-Se grains can be used strategically to deliver supranutritional Se and rapidly enhance Se depots in sheep, a task that does not seem attainable with Se salts. Furthermore, a 100-g portion of uncooked loin (LM) from the wethers fed supranutritional Se contained 196 to 250% of the recommended Se requirement for humans.
authors.affiliation: ARS, USDA, U.S. Sheep Experiment Station, Dubois, ID 83423, USA. btaylor@pw.ars.usda.gov
authors.forename: J B
authors.initials: JB
authors.lastname: Taylor
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001835:Body Weight; D004032:Diet / Q000662:veterinary*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009929:Organ Size; D011897:Random Allocation; D012643:Selenium / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D012756:Sheep / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014908:Triticum / Q000737:chemistry*; D014781:Viscera / Q000378:metabolism; D014935:Wool / Q000378:metabolism
chemical_list: D012643:Selenium