Rapid optical coherence tomography and recording functional scattering changes from activated frog retina.
Optical coherence tomography (OCT) has important potential advantages for fast functional neuroimaging. However, dynamic neuroimaging poses demanding requirements for fast and stable acquisition of optical scans. Optical phase modulators based on the electro-optic effect allow rapid phase modulation; however, applications to low-coherence tomography are limited by the optical dispersion of a broadband light source by the electro-optic crystal. We show that the optical dispersion can be theoretically estimated and experimentally compensated. With an electro-optic phase modulator-based, no-moving-parts OCT system, near-infrared scattering changes associated with neural activation were recorded from isolated frog retinas activated by visible light.
Yao
Xin-Cheng
XC
Biological and Quantum Physics, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Yamauchi
Angela
A
Perry
Beth
B
George
John S
JS
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D003199:Computer Systems; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D010775:Photic Stimulation / Q000379:methods; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology* / Q000502:physiology*; D011898:Ranidae; D012542:Scattering, Radiation; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*