Mechanism of a molecular valve in the halorhodopsin chloride pump.
Halorhodopsin is a light-driven chloride anion pump in which the trans-->cis photoisomerization of a retinal chromophore triggers a photocycle resulting in the translocation of chloride across the plasma membrane. The mechanism of chloride transfer past the cis retinal is determined here by computing multiple pathways for this process. The calculations reveal two conditions of the valve mechanism. First, a lumen absent in the ground state structure is transiently opened by chloride passage. Second, this activated opening, which is achieved by flexible deformation of the surrounding protein, is shown to significantly raise the chloride translocation barrier between photocycles, thus preventing chloride backflow. Unlike macroscopic valve designs, the protein allows differential ion flows in the pumping and resting states that are tuned to match the physiological timescales of the cell, thus creating a "kinetic" valve.
Gruia
Andreea D
AD
Computational Biochemistry, IWR, Heidelberg University, Im Neuenheimer Feld 368, D-69210 Heidelberg, Germany.
Bondar
Ana-Nicoleta
AN
Smith
Jeremy C
JC
Fischer
Stefan
S
D002712:Chlorides / Q000378:metabolism*; D025602:Halorhodopsins / Q000737:chemistry* / Q000378:metabolism
D002712:Chlorides; D025602:Halorhodopsins