[High-performance liquid chromatography combined with mass spectrum analysis for chromatographic fingerprinting of compound dandelion enema].
OBJECTIVE
To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases.


METHODS
Three-dimensional high-performance liquid chromatography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting.


RESULTS
By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde.


CONCLUSIONS
HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.
Yan
Yuan
Y
Department of Pharmaceutical Science, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Liu
Shi-Ting
ST
Xu
Zhong-Yuan
ZY
Luo
Qi-Zhi
QZ
Tan
Ya-Fei
YF
Chen
Zhi-Liang
ZL
D000285:Administration, Rectal; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D031205:Taraxacum / Q000737:chemistry*
D004365:Drugs, Chinese Herbal