title: Subcellular proteomic analysis of host-pathogen interactions using human monocytes exposed to Yersinia pestis and Yersinia pseudotuberculosis.
abstract: Yersinia pestis, the etiological agent of plague, is of concern to human health both from an infectious disease and a biodefense perspective. While Y. pestis and Yersinia pseudotuberculosis share more than 90% DNA homology, they have significantly different clinical manifestations. Plague is often fatal if untreated, yet Y. pseudotuberculosis causes severe intestinal distress but is rarely fatal. A better understanding of host response to these closely related pathogens may help explain the different mechanisms of virulence and pathogenesis that result in such different clinical outcomes. The aim of this study was to characterize host protein expression changes in human monocyte U937 cells after exposure to Y. pestis and Y. pseudotuberculosis. In order to gain global proteomic coverage of host response, proteins from cytoplasmic, nuclear and membrane fractions of host cells were studied by two-dimensional differential gel electrophoresis and relative protein expression differences were quantitated. Differentially expressed proteins, with at least 1.5-fold expression changes and p values of 0.01 or less, were identified by mass spectrometry including matrix-assisted laser desorption/ionization-MS or liquid chromatography tandem mass spectrometry. With these criteria, differential expression was detected in 16 human proteins after Y. pestis exposure and 13 human proteins after Y. pseudotuberculosis exposure, of which only two of the differentially expressed proteins identified were shared between the two exposures. Proteins identified in this study are reported to be involved in a wide spectrum of cellular functions and host defense mechanisms including apoptosis, cytoskeletal rearrangement, protein synthesis and degradation, DNA replication and transcription, metabolism, protein folding, and cell signaling. Notably, the differential expression patterns observed can distinguish the two pathogen exposures from each other and from unexposed host cells. The functions of the differentially expressed proteins identified provide insight on the different virulence and pathogenic mechanisms of Y. pestis and Y. pseudotuberculosis.
authors.affiliation: Biodefense Division, Lawrence National Laboratory, Livermore, CA 94550, USA.
authors.forename: Celia G
authors.initials: CG
authors.lastname: Zhang
authors.forename: Arlene D
authors.initials: AD
authors.lastname: Gonzales
authors.forename: Megan W
authors.initials: MW
authors.lastname: Choi
authors.forename: Brett A
authors.initials: BA
authors.lastname: Chromy
authors.forename: J Patrick
authors.initials: JP
authors.lastname: Fitch
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McCutchen-Maloney
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D020298:U937 Cells; D015010:Yersinia pestis; D015011:Yersinia pseudotuberculosis
chemical_list: D011480:Protease Inhibitors; D011506:Proteins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex