In vitro effect of cyclosporine A on juvenile Schistosoma mansoni labeled by AF18 (5-N-octadecanoyl aminofluorescein).
OBJECTIVE
To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity.


METHODS
Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18, then observe schistosomula under fluorescence microscope.


RESULTS
Schistosomula of different groups labeled by AF18 were damaged by cyclosporine A in vitro.


CONCLUSIONS
Cyclosporine A increases the uptake of AF18 by schistosomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.
Liu
Jian-fa
JF
Medical School of Ningbo University, Ningbo 315211 , China.
Kusel
John R
JR
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019793:Fluorescein / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000502:physiology
C116138:5-(N-octadecanoyl)aminofluorescein; D016572:Cyclosporine; D019793:Fluorescein