[Study on the interaction of copper-zinc superoxide dismutase with cobalt (II)-histidine by spectral analysis. II. Effects of pH, interaction time].
The direct interaction of Cu2Zn2SOD with organic metal compound (Cobalt (II)-Histidine) was studied by ICP, VIS and the measurement of enzyme activity, and also investigated the effect of pH values and different interaction times for this interaction. The results showed that with the increased in pH values and the more longer interaction times, the intensity of interaction were increased and the corresponding catalytic activity of enzyme were affected.
Zheng
X
X
Dalian Institute of Chemical Physics, Academia Sinica, 116023 Dalian.
Hu
J
J
Cheng
G
G
Xu
Y
Y
Zhao
Y
Y
Liu
M
M
D003035:Cobalt / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008667:Metalloproteins / Q000737:chemistry*; D011485:Protein Binding; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000737:chemistry*; D013997:Time Factors
D008667:Metalloproteins; D003035:Cobalt; D006639:Histidine; D013482:Superoxide Dismutase