A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler.
Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98.
Bhaskar
S Uday
SU
Department of Plant Molecular Biology and Biotechnology, Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, India.
Gopalaswamy
G
G
Raghu
R
R
D000645:Ammonium Sulfate / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods*; D002850:Chromatography, Gel; D000458:Cyanobacteria / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D009584:Nitrogen; D010798:Phycocyanin / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts; D000461:Rhodophyta / Q000378:metabolism; D013053:Spectrophotometry; D053545:Spirulina
D001426:Bacterial Proteins; D010936:Plant Extracts; D010798:Phycocyanin; D009584:Nitrogen; D000645:Ammonium Sulfate