title: Novel and simple high-performance liquid chromatographic method for determination of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity.
abstract: We present here a high-performance liquid chromatographic method for the evaluation of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity. The automated method was applied to fungal and mouse liver extracts and validated by the addition of mevastatin to the reaction mixture and by several intra- and inter-day assays. This method offers important advantages over those previously reported because no radiolabeled substrates or expensive techniques such as mass spectrometry are required, and the time of analysis is relatively short. Moreover, the method can be successfully applied to different biological samples; hence, it should be very useful in evaluating potential inhibitors of the HMG-CoA enzyme and investigating cholesterol metabolism, cell growth and differentiation processes.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino Carlo Bo, via A. Saffi 2, 61029 Urbino (PU), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Buffalini
authors.forename: Raffaella
authors.initials: R
authors.lastname: Pierleoni
authors.forename: Chiara
authors.initials: C
authors.lastname: Guidi
authors.forename: Paola
authors.initials: P
authors.lastname: Ceccaroli
authors.forename: Roberta
authors.initials: R
authors.lastname: Saltarelli
authors.forename: Luciana
authors.initials: L
authors.lastname: Vallorani
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005658:Fungi / Q000201:enzymology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006903:Hydroxymethylglutaryl CoA Reductases