Effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing: experimental study in rats.
OBJECTIVE
To analyze the effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing in rats.


METHODS
48 male Wistar rats, weighing 250 to 343g, were randomized into two groups: group I--bowel suture without applying a biologically absorbable membrane and group II--bowel suture with application of an absorbable membrane. The two groups were divided into subgroups of 3, 14 and 30 days of observation, with 8 rats in each subgroup. All were sacrificed after the end of the observation period.


RESULTS
No morbidity or mortality was observed during the experiment. The amounts of collagen in group I were 23.4%, 72.1% and 67.6% and in group II were 22.5%, 52.5% and 51.6%, for the subgroups of 3, 14 and 30 days, respectively. Comparison between groups showed that the 14-day (p = 0.0013) and 30-day (p = 0.0587) subgroups had significant variance, with larger collagen zones in animals in which the membrane was not applied. However, with regard to fibroblasts, group I had 2%, 13% and 8% and group II had 2%, 10% and 8%, for the 3-day (p = 1.0), 14-day (p = 0.3184) and 30-day (p = 0.5995) subgroups, respectively, showing no significant variance.


CONCLUSIONS
The use of the biologically absorbable membrane cause a decrease in collagen formation, while not altering the number of fibroblasts, in bowel suture healing in rats, without increased morbidity and mortality.
Perez
Antônio Carlos
AC
Postgraduate Program in Surgical Gastroenterology, Federal University of São Paulo (UNIFESP). acaperez@terra.com.br
Matos
Delcio
D
Montero
Edna Frasson de Souza
EF
de Souza
Heitor Soares
HS
Barbosa
Fábio Colagrossi Paes
FC
Saad
Fabio
F
Dip
Nelson
N
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000627:therapeutic use*; D003094:Collagen / Q000096:biosynthesis*; D003106:Colon / Q000601:surgery*; D005347:Fibroblasts; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013537:Sutures; D014945:Wound Healing / Q000187:drug effects*
D006820:Hyaluronic Acid; D003094:Collagen; D002266:Carboxymethylcellulose Sodium