title: Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs).
abstract: [structure: see text] The alpha-l-threofuranosyl nucleoside triphosphates of T, G, and D (tTTP, tGTP, and tDTP) were synthesized from the described 2'-O-DMT-protected derivatives using the Eckstein method, while the corresponding C derivative (tCTP) was prepared from the 2'-O-acetyl derivative. The prepared alpha-l-threofuranosyl nucleoside triphosphates, despite being one carbon shorter than the native 2'-deoxyfuranosyl nucleoside triphosphates, are effective substrates for selected DNA polymerases.
authors.affiliation: Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02184, USA.
authors.forename: Keyong
authors.initials: K
authors.lastname: Zou
authors.forename: Allen
authors.initials: A
authors.lastname: Horhota
authors.forename: Biao
authors.initials: B
authors.lastname: Yu
authors.forename: Jack W
authors.initials: JW
authors.lastname: Szostak
authors.forename: Larry W
authors.initials: LW
authors.lastname: McLaughlin
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009711:Nucleotides; D004259:DNA-Directed DNA Polymerase