Nucleobase participation in ribozyme catalysis.
We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are consistent with a role for nucleobase catalysis in the catalytic mechanism of this ribozyme.
Zhao
Zheng-Yun
ZY
CR-UK Nucleic Acid Structure Research Group, MSI/WTB Complex, University of Dundee, Dundee DD1 5EH, UK.
McLeod
Aileen
A
Harusawa
Shinya
S
Araki
Lisa
L
Yamaguchi
Maho
M
Kurihara
Takushi
T
Lilley
David M J
DM
D001483:Base Sequence; D002384:Catalysis; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism*; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012265:Ribonucleotides / Q000737:chemistry / Q000378:metabolism*
D007093:Imidazoles; D016337:RNA, Catalytic; D012265:Ribonucleotides; D012313:RNA; D004722:Endoribonucleases; C438195:varkud satellite ribozyme