title: A novel filtration-based processing method of liquid cytology specimens for human papillomavirus DNA testing by hybrid capture II.
abstract: We evaluated a more efficient method of processing liquid-based cervical cytology specimens for human papillomavirus (HPV) DNA testing by Hybrid Capture II (HCII). Aliquots were made from 701 specimens in the following sequence: 4.0, 2.0, 1.0, 0.5, and 1.5 mL. The 4.0-mL aliquot was processed by the standard method (STP), and half of the processed material was tested by HCII. Other aliquots were processed with a new, filtration-based processing method (NPM). The 2.0-mL NPM aliquot had HCII test performance most similar to the STP, ie, similar HCII positivity (P = .4) and good test agreement (kappa = 0.85, 95% confidence interval [CI], 0.80-0.89). The 194 cytologic negatives had greater positivity by STP (P = .04) compared with the 2.0-mL aliquot processed by NPM; between-method agreement was modest (kappa = 0.54, 95% CI, 0.36-0.72). A lower positive cut point for the 2.0-mL NPM aliquot partially abrogated this minor difference. In 241 specimens diagnosed as low-grade and 31 as high-grade squamous intraepithelial lesions, there were no significant differences in HPVpositivity (>85% and 90%, respectively) between STP and NPM. NPM reduces specimen handling and decreases total testing time by approximately 33% without significant losses in HCII test performance.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institute of Health, Department of Health and Human Services, Bethesda, MD 20892-7234, USA.
authors.forename: Philip E
authors.initials: PE
authors.lastname: Castle
authors.forename: Mariane
authors.initials: M
authors.lastname: Garcia-Meijide
authors.forename: E Blair
authors.initials: EB
authors.lastname: Holladay
authors.forename: Ronald
authors.initials: R
authors.lastname: Chuke
authors.forename: Jay
authors.initials: J
authors.lastname: Payne
authors.forename: Amanda
authors.initials: A
authors.lastname: Long
authors.forename: Heather
authors.initials: H
authors.lastname: Siefers
authors.forename: Franklin
authors.initials: F
authors.lastname: Demuth
authors.forename: Attila T
authors.initials: AT
authors.lastname: Lorincz
mesh_terms: D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral