Effective selection of transgenic papaya plants with the PMI/Man selection system.
The selectable marker gene phospho-mannose isomerase (pmi), which encodes the enzyme phospho-mannose isomerase (PMI) to enable selection of transformed cell lines on media containing mannose (Man), was evaluated for genetic transformation of papaya (Carica papaya L.). We found that papaya embryogenic calli have little or no PMI activity and cannot utilize Man as a carbon source; however, when calli were transformed with a pmi gene, the PMI activity was greatly increased and they could utilize Man as efficiently as sucrose. Plants regenerated from selected callus lines also exhibited PMI activity but at a lower specific activity level. Our transformation efficiency with Man selection was higher than that reported using antibiotic selection or with a visual marker. For papaya, the PMI/Man selection system for producing transgenic plants is a highly efficient addition to previously published methods for selection and may facilitate the stacking of multiple transgenes of interest. Additionally, since the PMI/Man selection system does not involve antibiotic or herbicide resistance genes, its use might reduce environmental concerns about the potential flow of those genes into related plant populations.
Zhu
Yun J
YJ
Hawaii Agriculture Research Center, 99-193 Aiea Heights Drive, Aiea, HI 96701, USA. jzhu@harc-hspa.com
Agbayani
Ricelle
R
McCafferty
Heather
H
Albert
Henrik H
HH
Moore
Paul H
PH
D000383:Agriculture / Q000379:methods; D019470:Biolistics; D029441:Carica / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D008358:Mannose / Q000378:metabolism / Q000494:pharmacology; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics*
D003470:Culture Media; D018744:DNA, Plant; D005819:Genetic Markers; D008359:Mannose-6-Phosphate Isomerase; D008358:Mannose