Novel tumor necrosis factor-knockout mice that lack Peyer's patches.
We generated a novel tumor necrosis factor (TNF) null mutation using Cre-loxP technology. Mice homozygous for this mutation differ from their "conventional" counterparts; in particular, they completely lack Peyer's patches (PP) but retain all lymph nodes. Our analysis of these novel TNF-knockout mice supports the previously disputed notion of the involvement of TNF-TNFR1 signaling in PP organogenesis. Availability of TNF-knockout strains both with and without PP enables more definitive studies concerning the roles of TNF and PP in various immune functions and disease conditions. Here, we report that systemic ablation of TNF, but not the presence of PP per se, is critical for protection against intestinal Listeria infection in mice.
Kuprash
Dmitry V
DV
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, USA. kuprash@online.ru
Tumanov
Alexei V
AV
Liepinsh
Dmitry J
DJ
Koroleva
Ekaterina P
EP
Drutskaya
Marina S
MS
Kruglov
Andrei A
AA
Shakhov
Alexander N
AN
Southon
Eileen
E
Murphy
William J
WJ
Tessarollo
Lino
L
Grivennikov
Sergei I
SI
Nedospasov
Sergei A
SA
D000818:Animals; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000276:immunology; D007150:Immunohistochemistry; D008088:Listeriosis / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics / Q000276:immunology*; D009154:Mutation; D010581:Peyer's Patches / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology
D014409:Tumor Necrosis Factor-alpha