Choroid plexus selectively accumulates T-lymphocytes in normal controls and after peripheral immune activation.
We determined T-lymphocyte migration into brain and choroid plexus (CPx) after enterotoxin-induced systemic immune activation. CPx T-lymphocytes/mm2 in control mice were > 3 logs more numerous than brain and increased by as much as 150-fold by post-enterotoxin Day 3 (p < 0.01). Flow cytometry of pooled CPx confirmed post-enterotoxin increases. Brain T-lymphocytes increased up to 17-fold after SEB and accumulated in subependymal and periventricular brain. T cell apoptosis was absent. These results show preferential T-lymphocyte migration to CPx over brain and suggest that brain T cells may be derived from the CPx by direct migration or by cerebrospinal fluid dissemination.
Petito
C K
CK
Department of Pathology, University of Miami, School of Medicine, 1550 NW 10th Avenue, Miami, FL 33136, USA. cpetito@med.miami.edu
Adkins
B
B
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000187:drug effects; D017252:CD3 Complex / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002831:Choroid Plexus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004768:Enterotoxins / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D017252:CD3 Complex; D004768:Enterotoxins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C031818:enterotoxin B, staphylococcal