title: Mutation analysis of the preproghrelin gene: no association with obesity and type 2 diabetes.
abstract: OBJECTIVE
  To investigate the preproghrelin gene for variants and their association with obesity and type 2 diabetes.
  
  
  METHODS
  82 obese probands were analyzed for mutations using single-strand conformational polymorphism, heteroduplex analyses and sequencing. Association studies were performed in 234 juvenile-onset obese and 323 lean men and in 557 type 2 diabetic and 233 glucose tolerant subjects.
  
  
  RESULTS
  We identified two novel variants, 36C > T and IVS3 + 715delC, and 4 known variants, Arg51Gln, Leu72Met, Gln90Leu, and IVS1 + 169G > A. None of the variants showed any significant association with obesity or type 2 diabetes or estimates of glucose and lipid metabolism in glucose tolerant subjects.
  
  
  CONCLUSIONS
  Variation in the preproghrelin gene is not associated with juvenile-onset obesity, type 2 diabetes or related phenotypes among the examined Danish Caucasian subjects.
authors.affiliation: Steno Diabetes Center and Hagedorn Research Institute, Copenhagen, Denmark. LeiL@steno.dk
authors.forename: Lesli H
authors.initials: LH
authors.lastname: Larsen
authors.forename: Anette P
authors.initials: AP
authors.lastname: Gjesing
authors.forename: Thorkild I A
authors.initials: TI
authors.lastname: Sørensen
authors.forename: Yasmin H
authors.initials: YH
authors.lastname: Hamid
authors.forename: Søren M
authors.initials: SM
authors.lastname: Echwald
authors.forename: Søren
authors.initials: S
authors.lastname: Toubro
authors.forename: Eva
authors.initials: E
authors.lastname: Black
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
authors.forename: Torben
authors.initials: T
authors.lastname: Hansen
authors.forename: Oluf
authors.initials: O
authors.lastname: Pedersen
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D004252:DNA Mutational Analysis / Q000379:methods; D003718:Denmark; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005838:Genotype; D054439:Ghrelin; D006801:Humans; D008297:Male; D008875:Middle Aged; D009037:Motilin / Q000235:genetics*; D009765:Obesity / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D054439:Ghrelin; D009037:Motilin