[Analysis of the sequences of the ribosomal DNA ITS2 region for differentiating anopheline mosquitoes within hyrcanus group].
OBJECTIVE
To analyze the genetic characteristics of ribosomal DNA ITS2 region for differentiating anopheline mosquitoes within Hyrcanus group.


METHODS
The ribosomal DNA ITS2 region of both laboratory line and filed collected An. anthropophagus and An. sinensis as well as the field collected An. yatsushiroensis and An. lesteri were amplified and sequenced. The sequencing data were then analyzed for the restriction mapping using Omega Sequencing analysis program.


CONCLUSIONS
The length of the sequences of An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri are 472, 452, 456 and 456 bp respectively. The restriction mapping showed that there were different restriction digesting sites among the ribosomal DNA ITS2 region sequences from An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri. On the basis of the sequence differences among the anopheline species within Hyrcanus group, it is possible to develop new technique for genetic identification of anopheline mosquitoes.
Gao
Qi
Q
Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
Zhou
Hua-yun
HY
Li
Ju-lin
JL
Li
Feng-hua
FH
Choe
Tong Gyu
TG
Yom
Sung Chan
SC
Zhu
Guo-ding
GD
Cao
Jun
J
D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D021903:DNA, Ribosomal Spacer