Autoantibodies against the amino terminal of alpha-enolase are a useful diagnostic marker of Hashimoto's encephalopathy.
We investigated autoantibodies and their epitope(s) in Hashimoto's encephalopathy associated with Hashimoto's thyroiditis. In a proteomic analysis, they proved to recognize alpha-enolase. We further searched the epitope region in alpha-enolase using different regions of recombinant proteins expressed in cultured human cells. The amino terminal region was recognized by autobodies from a much higher proportion of patients with Hashimoto's encephalopathy (83.3%; 5/6) than from patients with Hashimoto's thyroiditis (11.8%; 2/17), and not at all by sera from controls (25 healthy individuals and 25 controls with other neurological disorders) (0%; 0/50). Neither the carboxyl terminal nor the mid-region of alpha-enolase showed specificity for Hashimoto's encephalopathy. Autoantibodies against the amino terminal of alpha-enolase are a useful diagnostic marker for Hashimoto's encephalopathy.
Fujii
A
A
Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
Yoneda
M
M
Ito
T
T
Yamamura
O
O
Satomi
S
S
Higa
H
H
Kimura
A
A
Suzuki
M
M
Yamashita
M
M
Yuasa
T
T
Suzuki
H
H
Kuriyama
M
M
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers; D014408:Biomarkers, Tumor; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D004452:Echocardiography / Q000379:methods; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D025521:Tumor Suppressor Proteins / Q000097:blood / Q000737:chemistry / Q000276:immunology*
D001323:Autoantibodies; D015415:Biomarkers; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase