title: Preparation and characterization of porous beta-tricalcium phosphate/collagen composites with an integrated structure.
abstract: Porous beta-tricalcium phosphate (TCP)/collagen composites with different beta-TCP/collagen weight ratio were prepared. The influences of the preparation conditions on the microstructure of porous composite and the joint status of beta-TCP particles with collagen fibrils were characterized by X-ray diffractometer, scanning electron microscopy and transmission electron microscopy. The results showed: (1) an acid treatment could effectively disassemble collagen fibrils; (2) in the resulting porous composites, beta-TCP particles homogenously existed on the skeleton of the collagen fibril network and bonded tightly to both the fibrils and themselves. The tight bonding formation could be due to the reaction between Ca ions in the particles and carboxyl groups in collagen polypeptide chains and due to the reprecipitation of partially dissolved beta-TCP during synthesis. The tight bonding between beta-TCP particles and collagen fibrils in the composites demonstrated an integrated structure, which was reproducible when beta-TCP/collagen ratio ranged from 2 to 4. Such integrated structure would make significant contributions in reliably tailoring properties of the porous composites by varying beta-TCP content. In addition, the porous composites had large porosity (approximately 95%) and appropriate pore size (approximately 100 microm), showed no negative impact in cytotoxicity assay and complete bone tissue regeneration after 12 weeks in animal test.
authors.affiliation: Department of Materials Science and Engineering, Zhejiang University, Hangzhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zou
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.forename: Xinggang
authors.initials: X
authors.lastname: Liu
authors.forename: Piyi
authors.initials: P
authors.lastname: Du
authors.forename: Ge
authors.initials: G
authors.lastname: Shen
authors.forename: Gaorong
authors.initials: G
authors.lastname: Han
mesh_terms: D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D018786:Bone Substitutes / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D003094:Collagen / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology* / Q000648:ultrastructure; D003460:Crystallization / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008420:Manufactured Materials / Q000032:analysis; D008422:Materials Testing; D051379:Mice; D008968:Molecular Conformation; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D011485:Protein Binding; D011817:Rabbits; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D003094:Collagen