title: Direct correlation between adsorption-induced changes in protein structure and platelet adhesion.
abstract: It is widely recognized that adsorbed proteins on biomaterial surfaces tend to initiate thrombus formation, although the specific mechanisms involved are still not well understood. In attempts to decrease the conformational change of adsorbed proteins, surface treatments that reduce surface hydrophobicity have been considered, such as the sulfonation of low-density polyethylene and isotactic polypropylene. The objectives of this present research were to study how changes in surface chemistry influence the degree of conformational change of adsorbing proteins and to investigate the correlation between the change in adsorbed protein structure and platelet response. Adsorbed porcine serum albumin and porcine fibrinogen were used as the model proteins for determining the effects of sulfonation on protein conformational change. Circular dichroism spectroscopy studies showed that the proteins were less altered structurally on the sulfonated surfaces. Platelet adhesion studies were used to correlate the number of adhered platelets with the amount of conformational change in adsorbed proteins on the polymer surface. The results of these studies show a linear correlation between platelet adhesion and the degree of adsorption-induced protein conformational change. These findings suggest that the degree of protein conformational change after adsorption is a dominant mechanism governing platelet interactions with biomaterial surfaces.
authors.affiliation: Department of Bioengineering, 501 Rhodes Engineering Research Center, Clemson University, South Carolina 29634, USA.
authors.forename: Donna M
authors.initials: DM
authors.lastname: Hylton
authors.forename: Shalaby W
authors.initials: SW
authors.lastname: Shalaby
authors.forename: Robert A
authors.initials: RA
authors.lastname: Latour
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D005340:Fibrinogen / Q000737:chemistry*; D010973:Platelet Adhesiveness; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D012709:Serum Albumin; D013451:Sulfonic Acids; D005340:Fibrinogen