Configuration of a new bioartificial liver support system and in vitro evaluation of its functions.
The aim of this study was to configure a new bioartificial liver (BAL) support system and evaluate its functions in vitro. Chinese experimental miniature pig hepatocytes were isolated by an in situ recirculating collagenase perfusion method and were cultured in serum-free medium with restriction attachment and spinner technique to form hepatocyte spheroid suspensions containing 1.0 x 10(10) hepatocytes. The BAL support system was configured by inoculating the hepatocyte spheroids into the cell circuit of a hollow fiber bioreactor (BIOLIV A3A, Cell Biotech Limited, HK, China). The number and viability of hepatocytes, the levels of alanine aminotransferase (ALT), total bilirubin (TBi), and albumin (ALB) in the circulating hepatocyte suspension and RPMI-1640 medium, and lidocaine metabolism were determined during 6 hr of circulation in the BAL devices. Independent experiments were performed 5 times. There were no significant changes in the number and viability of the hepatocytes during the circulation period. The BAL support system demonstrated substantial albumin synthesis and lidocaine metabolism. The results indicate that the new BAL support system has the ability to perform liver functions and could be used to treat liver failure or provide temporary liver support in patients who are candidates for liver transplantation.
Chen
Zhong
Z
National Key Lab of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing People's Republic of China. zchen9999@sina.com.cn
Ding
Yitao
Y
Li
Genxi
G
D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D008012:Lidocaine / Q000493:pharmacokinetics; D019164:Liver, Artificial; D008954:Models, Biological; D013552:Swine
D008012:Lidocaine