Resistance of paramyxoviridae to type I interferon-induced Bos taurus Mx1 dynamin.
Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae family. Recently, bovine Mx1 protein (BoMx1) was included in the group of Mx proteins with authenticated antiviral potential, as it dramatically represses the replication of vesicular stomatitis virus (VSV). As replication in bovine cells of Pi3, respiratory syncytial (RS), and Sendai (Se) viruses, all members of the same family, is known to be reduced on IFN-alpha incorporation into the culture medium, it was hypothesized that the BoMx1 pathway possibly was involved, its antiviral spectrum thus probably extending to Paramyxoviridae. In this study, probing of BoMx1-inhibiting effects was carried out by infecting a transgenic Vero cell line that allows tightly regulated conditional expression of BoMx1 after doxycycline treatment with a wide array of Paramyxoviridae. Expressing and nonexpressing cells displayed similar viability, cytopathic effects (CPEs), and amounts of infectious virus yields, whatever the infecting virus or the multiplicity of infection (moi) imposed. It is, therefore, concluded that BoMx1 does not interfere with Paramyxoviridae.
Leroy
Michael
M
Department of Pathology, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Baise
Etienne
E
Pire
Grégory
G
Gérardin
Joel
J
Desmecht
Daniel
D
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism; D002417:Cattle / Q000378:metabolism* / Q000821:virology; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D034281:Dynamins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007370:Interferon Type I / Q000502:physiology; D064547:Myxovirus Resistance Proteins; D010252:Paramyxoviridae / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism
D000998:Antiviral Agents; D007370:Interferon Type I; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D014764:Viral Proteins; D019204:GTP-Binding Proteins; D034281:Dynamins