title: Influence of temperature and nutritional requirements for mycelial growth of Eutypa lata, a vineyard pathogenic fungus.
abstract: Eutypa dieback (dying arm disease, eutypiosis) is a very devastating disease in many grape-producing areas around the world. This vascular disease is induced by the ascomycete Eutypa lata Pers. Fr. Tul & C. Tul. invading the trunk by pruning wounds. The environmental factors and the nutritional requirements regulating fungus development are yet poorly known. This work shows that the isolated strain of E. lata was able to grow in a large temperature range (2-30 degrees C). However, a higher temperature (35 degrees C) presented inhibitory effects on mycelial growth. E. lata was able to use various osidic molecules (C5, C6, C12, C18, C24, and starch); showing thus a large adaptation to the carbon source supplied. As nitrogen source, it used salts and numerous natural amino acids. A significant result was obtained with cysteine presenting obvious antifungal properties. This effect can further be used with the aim of setting up a curative treatment of the disease.
authors.affiliation: Laboratoire de biochimie, physiologie et biologie moléculaire végétales, UMR CNRS 6161, université de Poitiers, bât. Botanique, 40, av. du Recteur-Pineau, 86022 Poitiers, France.
authors.forename: Bénigne-Ernest
authors.initials: BE
authors.lastname: Amborabé
authors.forename: Stéphane
authors.initials: S
authors.lastname: Octave
authors.forename: Gabriel
authors.initials: G
authors.lastname: Roblin
mesh_terms: D001203:Ascomycota / Q000254:growth & development*; D025282:Mycelium / Q000254:growth & development*; D010935:Plant Diseases / Q000382:microbiology*; D013696:Temperature; D027843:Vitis