Compartmentalization of HIV-1 in the central nervous system: role of the choroid plexus.
OBJECTIVE
To determine the genetic and phenotypic composition of HIV-1 found in the choroid plexus (CPx) and its relationship to virus in the brain and peripheral lymphoid tissue.


METHODS
Phenotypic and molecular comparisons of HIV-1 found in autopsy brain, CPx, and spleen tissues.


METHODS
HIV-1 was co-cultured from matched postmortem brain (basal ganglia), CPx, and spleen tissues of AIDS patients with and without HIV-associated encephalitis and dementia. Viral phenotypes were determined by infection of monocyte-derived macrophages, MT-2 or co-receptor-specific cell lines. Viral env and pol sequences were determined from genomic DNA isolated directly from tissues or co-cultures, and phylogenetic comparisons were performed.


RESULTS
CCR5-utilization was the most prevalent viral tropism found in all tissues, although spleen isolates also displayed CXCR4 usage. Viruses isolated from CPx consisted of both peripheral and brain-like virus, but were more related phenotypically and genetically to those found in the brain. Mutations found in the pol gene that could confer drug resistance to brain and CPx isolates were similar to those found in the periphery.


CONCLUSIONS
The CPx contained replication-competent virus that was most similar, although distinct, from that found in the brain. It also contained some viruses with high similarity to those of peripheral origin. Compartmentalization of viral env and pol sequences indicated that differential selective pressures exist in each tissue examined. These studies suggest that the CPx may provide an environment that promotes the evolution of drug-resistant strains with central nervous system tropism, although it is unlikely to be a reservoir for archival HIV-1 variants.
Burkala
Evan J
EJ
Nebraska Center for Virology and the School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
He
Jun
J
West
John T
JT
Wood
Charles
C
Petito
Carol K
CK
D015526:AIDS Dementia Complex / Q000821:virology; D000328:Adult; D001479:Basal Ganglia / Q000821:virology; D001483:Base Sequence; D016022:Case-Control Studies; D002490:Central Nervous System / Q000821:virology*; D002831:Choroid Plexus / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D015752:Genes, env; D015751:Genes, pol; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000821:virology; D008875:Middle Aged; D008969:Molecular Sequence Data; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D013154:Spleen / Q000821:virology; D014776:Virus Cultivation
D004279:DNA, Viral; D019713:Receptors, CCR5; D019718:Receptors, CXCR4