Functional role of AMP-activated protein kinase in the heart during exercise.
AMP-activated protein kinase (AMPK) plays a critical role in maintaining energy homeostasis and cardiac function during ischemia in the heart. However, the functional role of AMPK in the heart during exercise is unknown. We examined whether acute exercise increases AMPK activity in mouse hearts and determined the significance of these increases by studying transgenic (TG) mice expressing a cardiac-specific dominant-negative (inactivating) AMPKalpha2 subunit. Exercise increased cardiac AMPKalpha2 activity in the wild type mice but not in TG. We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity.
Musi
Nicolas
N
Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA.
Hirshman
Michael F
MF
Arad
Michael
M
Xing
Yanqiu
Y
Fujii
Nobuharu
N
Pomerleau
Jason
J
Ahmad
Ferhaan
F
Berul
Charles I
CI
Seidman
Jon G
JG
Tian
Rong
R
Goodyear
Laurie J
LJ
D055372:AMP-Activated Protein Kinases; D000818:Animals; D004452:Echocardiography; D006321:Heart / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009097:Multienzyme Complexes / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D009097:Multienzyme Complexes; D017346:Protein Serine-Threonine Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases