title: Study of enrofloxacin depletion in the eggs of laying hens using diphasic dialysis extraction/purification and determinative HPLC-MS analysis.
abstract: A study of the depletion of enrofloxacin residues in eggs was carried out using a diphasic dialysis procedure for the extraction of fluoroquinolone residues from the matrix. Enrofloxacin was administered to laying hens through the intramuscular route (15 mg/day) and orally (12 mg/day). After daily collection, the egg albumen and the egg yolk were separated, and the residue levels were determined using an HPLC-MS (API-ESI) method. The enrofloxacin and ciprofloxacin peaks gradually increased until the fifth day, because the drug was employed for 5 days. However, differences were observed in the depletion curves of enrofloxacin and its metabolite when both parts of the egg and the mode of administration were considered.
authors.affiliation: Laboratorio de Higiene, Inspección y Control de Alimentos, Area de Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, E-27002 Lugo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lolo
authors.forename: S
authors.initials: S
authors.lastname: Pedreira
authors.forename: C
authors.initials: C
authors.lastname: Fente
authors.forename: B I
authors.initials: BI
authors.lastname: Vázquez
authors.forename: C M
authors.initials: CM
authors.lastname: Franco
authors.forename: A
authors.initials: A
authors.lastname: Cepeda
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis; D003956:Dialysis; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000032:analysis*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D015363:Quinolones / Q000008:administration & dosage / Q000032:analysis*
chemical_list: D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin