title: Multilocus variable-number tandem repeat typing of Mycobacterium ulcerans.
abstract: The apparent genetic homogeneity of Mycobacterium ulcerans contributes to the poorly understood epidemiology of M. ulcerans infection. Here, we report the identification of variable number tandem repeat (VNTR) sequences as novel polymorphic elements in the genome of this species. A total of 19 potential VNTR loci identified in the closely related M. marinum genome sequence were screened in a collection of 23 M. ulcerans isolates, one Mycobacterium species referred to here as an intermediate species, and five M. marinum strains. Nine of the 19 loci were polymorphic in the three species (including the intermediate species) and revealed eight M. ulcerans and five M. marinum genotypes. The results from the VNTR analysis corroborated the genetic relationships of M. ulcerans isolates from various geographical origins, as defined by independent molecular markers. Although these results further highlight the extremely high clonal homogeneity within certain geographic regions, we report for the first time the discrimination of the two South American strains from Surinam and French Guyana. These findings support the potential of a VNTR-based genotyping method for strain discrimination within M. ulcerans and M. marinum.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ablordey
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.forename: Christine
authors.initials: C
authors.lastname: Hubans
authors.forename: Karim
authors.initials: K
authors.lastname: Chemlal
authors.forename: Camille
authors.initials: C
authors.lastname: Locht
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
authors.forename: Philip
authors.initials: P
authors.lastname: Supply
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D019910:Mycobacterium marinum / Q000145:classification / Q000235:genetics; D019911:Mycobacterium ulcerans / Q000145:classification* / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*