Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.
A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
Guilbaud
Morgan
M
Laboratoire de Microbiologie Alimentaire et Industrielle, ENITIAA, Nantes, France.
de Coppet
Pierre
P
Bourion
Fabrice
F
Rachman
Cinta
C
Prévost
Hervé
H
Dousset
Xavier
X
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D052160:Benzothiazoles; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003959:Diamines; D006360:Heat-Shock Proteins / Q000235:genetics; D006460:Hemolysin Proteins; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D052160:Benzothiazoles; D017931:DNA Primers; D004269:DNA, Bacterial; D003959:Diamines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; C038705:hlyA protein, Listeria monocytogenes