Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.
Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
Birtles
Andrew
A
Health Protection Agency, Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK.
McCarthy
Noel
N
Sheppard
Carmen L
CL
Rutter
Harry
H
Guiver
Malcolm
M
Haworth
Elizabeth
E
George
Robert C
RC
D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D017809:Fatal Outcome; D006703:Ill-Housed Persons; D006801:Humans; D008297:Male; D008586:Meningitis, Pneumococcal / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013301:Streptolysins / Q000235:genetics*
D001426:Bacterial Proteins; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae