Functional analysis of cells obtained from bronchoalveolar lavage fluid (BALF) of lung cancer patients.
BALF from tumor segments provides access to immune system cells in contact with lung tumors. We analyzed BALF cells as to their production of H2O2 and NO, comparing tumor-affected to non-affected lung segments. Twelve patients were studied (4 NSCLC, 3 SCC, 5 Adenocarcinoma). The cell numbers recovered from BALF varied, and, in adenocarcinoma patients, smaller numbers were recovered from tumor-affected segments. H2O2 production (up to 6.3 nmoles/2x10(5)cells) was obtained in 7/12 patients and, in these, it was more frequent in non-affected segments (7/7) than in affected segments (2/7). After culture, NO production was observed in three patients (6 to 314 microM) that also produced H2O2. These functional characteristics of cells in contact with neoplasia may have a role in determining the fate of the interactions between the immune system and lung cancer.
Anselmo
Luciene Barbosa
LB
Departamento de Imunologia Instituto de Ciências Biomédicas-USP, Av Prof Lineu Prestes 1730 - CEP 05508-000 - Cidade Universitária - São Paulo - SP - Brazil.
Gross
Jefferson Luiz
JL
Haddad
Fabio
F
Deheinzelin
Daniel
D
Younes
Riad Naim
RN
Barbuto
José Alexandre Marzagão
JA
D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*
D009569:Nitric Oxide; D006861:Hydrogen Peroxide