title: Ets-1 expression in gastric cancer.
abstract: OBJECTIVE
  Ets-1 protein is a transcription factor that has been implicated in both tumor invasion and neovascularization. The purpose of this study was to investigate the clinical significance of Ets-1 expression in gastric cancer.
  
  
  METHODS
  We examined the expression of Ets-1 protein by immunohistochemical staining in tissues obtained from 124 primary gastric cancers. Ets-1 expression was detected by automated immunodetection (Ventana Medical Systems Inc., Tucon, AZ, USA).
  
  
  RESULTS
  Ets-1 was not expressed in normal gastric epithelium. Ets-1 staining was defined as positive in 51.6% (64 of 124) of gastric cancer cases. There were no significant differences in age, gender, or histopathological grading. With regard to clinicopathological characteristics, significant differences were observed in the depth of tumor invasion, lymph node and distant metastases, increased tumor stage, and histological lymphatic and venous invasion. Patients with Ets-1 expression demonstrated poorer survival rates than patients without Ets-1 expression, as determined by the log-rank test (p=0.017), but multivariable analysis showed that the expression of Ets-1 was not an independent prognostic indicator (p=0.370).
  
  
  CONCLUSIONS
  Ets-1 expression in gastric cancers is correlated with histological prognostic indicators and is a useful marker for predicting the outcome for patients with gastric cancer.
authors.affiliation: Department of General Surgical Science (Surgery I), Gunma University, Graduate School of Medicine, Maebashi, Gunma, Japan. chuchumi@showa.gunma-u.ac.jp
authors.forename: Soichi
authors.initials: S
authors.lastname: Tsutsumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
authors.forename: Nikuo
authors.initials: N
authors.lastname: Nagashima
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shimura
authors.forename: Erito
authors.initials: E
authors.lastname: Mochiki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors