Plasma level of lipid peroxidation, total sulphydryl groups and nitric oxide levels in cancer patients irradiated on different anatomic fields: a case-control study.
OBJECTIVE
The aim of this study was to evaluate the effect of radiotherapy on lipid peroxidation, total sulfhydryl groups (RSH) and nitric oxide (NO radical) levels in plasma of cancer patients treated on different anatomic sites, and to compare the results with control subjects.


METHODS
89 patients with various malignancies and 33 healthy control subjects were recruited for the study. Cancer patients were grouped according to the irradiation portals (head and neck, thoracic, breast, abdominal and pelvic). Two blood samples before and after the completion of the fifth week of radiotherapy were collected from each patient.


RESULTS
Plasma levels of malondialdehyde (MDA) and NO radical of cancer patients before irradiation were found higher than those in control subjects. After irradiation, a marked lipid peroxidation increase was demonstrated as well as the significant decrease of plasma RSH level.


CONCLUSIONS
The alterations of the parameters indicate enhanced oxidant stress and point to the necessity of antioxidant prophylaxis upon application of radiotherapy.
Unsal
Diclehan
D
Department of Radiation Oncology, Gazi University Faculty of Medicine, 06510 Ankara, Turkey. diclehan@yahoo.com
Akmansu
Muge
M
Ozer
Cigdem
C
Gonul
Bilge
B
Bora
Huseyin
H
D000367:Age Factors; D005260:Female; D005609:Free Radicals / Q000097:blood / Q000528:radiation effects; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000528:radiation effects; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000532:radiotherapy*; D009569:Nitric Oxide / Q000097:blood / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects; D011878:Radiotherapy / Q000009:adverse effects*; D013438:Sulfhydryl Compounds / Q000097:blood / Q000528:radiation effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000528:radiation effects
D005609:Free Radicals; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D008315:Malondialdehyde