Relationship between plasma leptin, insulin and tumor necrosis factor alpha in obese children.
OBJECTIVE
1. To evaluate the relationship between plasma leptin and TNFalpha concentrations in obese children and to assess the differences between hyperinsulinemic and normoinsulinemic groups. 2. To evaluate the relationship between plasma leptin and insulin levels in obese children. 3. To investigate the TNFalpha G308A mutation in obese children.


METHODS
Body mass index (BMI), fasting plasma glucose and insulin levels, oral glucose tolerance test results, homeostasis model assessment of insulin resistance (HOMA-IR) results, and plasma leptin and TNFalpha concentrations were evaluated in obese children (n = 45) and age- and gender-matched, lean healthy controls (n = 40).


RESULTS
In obese children the fasting insulin, HOMA-IR results, plasma leptin and TNFalpha concentrations were significantly higher than in controls (p <0.05). Furthermore, obese females showed higher plasma leptin and insulin resistance compared to obese males. While plasma leptin, TNFalpha levels and HOMA-IR results were similar in the prepubertal and pubertal groups, insulin levels were significantly higher in the pubertal group. Plasma leptin and TNFalpha concentrations were similar in hyperinsulinemic and normoinsulinemic obese children. In control children, plasma leptin concentrations showed a positive correlation with BMI, age, fasting insulin and HOMA-IR results. In obese children, plasma leptin levels did not correlate with BMI, fasting insulin or TNFalpha.


CONCLUSIONS
Plasma leptin concentrations did not show any correlation with TNFalpha levels in obese children. Furthermore, plasma leptin and TNFalpha concentrations were similar in hyperinsulinemic and normoinsulinemic obese children.
Aycan
Zehra
Z
Department of Pediatric Endocrinology, Ankara University School of Medicine, Ankara, Turkey. zehraaycan67@hotmail.com
Berberoğlu
Merih
M
Ocal
Gönül
G
Evliyaoglu
Olcay
O
Adiyaman
Pelin
P
Deda
Gülhis
G
Caksen
Hüseyin
H
Akar
Nejat
N
Karahan
Ceren
C
Cinas
Peyami
P
Bideci
Aysun
A
D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D005215:Fasting; D005260:Female; D006706:Homeostasis; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000276:immunology* / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha