title: RAP, an integrated program for monitoring bioluminescence and analyzing circadian rhythms in real time.
abstract: To process the large number of circadian rhythmic bioluminescence data that we generated with specially developed, high-throughput monitoring apparatuses, we developed an integrated rhythm-analyzing program (RAP) with a user-friendly graphical interface. RAP does all of the following in real time: (i) displays the bioluminescence time course, (ii) records time-series data, (iii) analyzes the data, (iv) displays the analyzed results, (v) statistically evaluates the analyzed results, and (vi) provides printouts. Because RAP can import files, it can analyze not only bioluminescence data but also other data such as those obtained by DNA array experiments and by Northern and Western blot analyses. The program is a powerful tool for large-scale investigations of biological rhythmic phenomena in general.
authors.affiliation: Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Okamoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Onai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishiura
mesh_terms: D017360:Arabidopsis / Q000502:physiology; D015152:Blotting, Northern; D015153:Blotting, Western; D002940:Circadian Rhythm; D008163:Luminescent Measurements / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D012984:Software; D046940:Synechococcus / Q000502:physiology