Surface properties of Yarrowia lipolytica and their relevance to gamma-decalactone formation from methyl ricinoleate.
The surface of the lipid-degrading yeast, Yarrowia lipolytica, was characterized by contact angle and zeta potential (zeta) measurements. The cells were mainly hydrophilic with a negative charge that was only affected from pH 2 to 4. To study the effects of the surface charges on the biotransformation of methyl ricinoleate into the aroma compound, gamma-decalactone, the zeta values of the substrate droplets were modified by adding a cationic surfactant into the medium at concentrations that did not diminish cell viability: the adhesion of the lipid substrate to the cells was increased but not the overall performance of the process, therefore the adhesion is not the rate limiting here. Our methodology offers interesting perspectives for further applications.
Aguedo
Mario
M
Centro de Engenheria Biológica, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Waché
Yves
Y
Belin
Jean-Marc
JM
Teixeira
José A
JA
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007783:Lactones / Q000378:metabolism*; D050356:Lipid Metabolism; D012277:Ricinoleic Acids / Q000378:metabolism*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000494:pharmacology; D025062:Yarrowia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D002593:Cetrimonium Compounds; D007783:Lactones; D012277:Ricinoleic Acids; D013501:Surface-Active Agents; C109757:decan-4-olide; C005235:methyl ricinoleate; D000077286:Cetrimonium