title: Prevalence and molecular characterization of Giardia duodenalis from sheep in central Italy.
abstract: Giardiosis in domestic ruminants is an important parasitic disease and it has been shown to impair growth in lambs, thus constituting a disease of economic concern. In Europe, surveys on the prevalence of giardiosis in sheep are limited. In order to obtain additional information on the presence of giardiosis and on the potential zoonotic role of Giardia duodenalis affecting sheep in central Italy, faecal samples of 325 native sheep from 20 farms in Abruzzo region (Italy) were examined for the presence of Giardia and the isolates were genotyped and sequenced. G. duodenalis cysts were detected in five of the 325 sheep (1.5%) (mean of 450 cysts/g) coming from two farms. The 770-bp fragment of the glutamate dehydrogenase gene and the 753-bp fragment of the beta-giardin gene showed 100% homology with the Assemblage AI. This work suggests for the first time in Italy that sheep carry a Giardia genotype, which can be a potential public health hazard.
authors.affiliation: Department of Production Science, Engineering, Mechanics and Economy, Faculty of Agriculture, University of Foggia, Foggia, Italy. a.giangaspero@unifg.it
authors.forename: Annunziata
authors.initials: A
authors.lastname: Giangaspero
authors.forename: Barbara
authors.initials: B
authors.lastname: Paoletti
authors.forename: Raffaella
authors.initials: R
authors.lastname: Iorio
authors.forename: Donato
authors.initials: D
authors.lastname: Traversa
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis; D005872:Giardia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan