Analysis of cellular fatty acids in Orientia tsutsugamushi as taxonomic markers.
Six Orientia strains including 3 prototype strains such as Gilliam, Karp, and Kato, and 3 strains (Boryong, Pajoo, and Yongworl) isolated in Korea, were studied for the profiles of their cellular fatty acids. All tested strains contained octadecenoic acid C (18: 1) omega 9 c(57.3 +/- 3.5%), octadecanoic acid C (18: 0) (15.3 +/- 1.5%), and hexadecanoic acid C (16: 0) (12.7 +/- 1.7%) as major components; however, interestingly, eicosenoic acid C (20: 1) omega 9 c(2.6 +/- 0.6%) was found in all strains except the Yongworl strain. Furthermore none of the strains contained 3-hydroxy fatty acids. The ratios of total saturated fatty acid (SFA) to total unsaturated fatty acid (UFA) were within the range of 0.34 to 0.54. These results showed that the cellular fatty acid profile should provide more reliable information for the identification of these bacteria.
Shim
Soo-Kyoung
SK
Division of Rickettsial and Zoonotic Diseases, Department of Bacteriology, National Institute of Health, Seoul, Korea.
Shin
Yong-Kook
YK
Choi
Eun-Na
EN
Han
Bok-Ghee
BG
Lee
Hae-Kyung
HK
Choi
Young-Sill
YS
Lee
Byung-Chul
BC
Yoon
Kwon-Sang
KS
Park
Mi-Yeoun
MY
D015415:Biomarkers; D004537:Eicosanoic Acids / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D007723:Korea; D009829:Oleic Acids / Q000032:analysis / Q000737:chemistry; D012285:Orientia tsutsugamushi / Q000737:chemistry* / Q000145:classification*; D019308:Palmitic Acid / Q000032:analysis / Q000737:chemistry
D015415:Biomarkers; D004537:Eicosanoic Acids; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D019308:Palmitic Acid