Tissue microarray analysis of C-MYC oncogene copy number changes in larynx carcinoma.
OBJECTIVE
Copy number changes and structural rearrangements of 8q in tumors from the head and neck region have been reported by numerous studies. To give better insights into the role of 8q abnormalities in larynx carcinoma, C-MYC copy number changes were studied in a large number of tumor materials.


METHODS
Fluorescent in situ hybridization with DNA locus-specific probe for C-MYC genewas applied on tissue microarrays presented by 863 larynx tumors.


RESULTS
32.02% had increased copy number of C-MYC. Of these, 6.16% hadamplification and 25.86% had C-MYC gains. The analysis revealed a significant difference in the frequency of gains in primary and advanced tumors. Furthermore, we observed an association of gains with advanced clinical stage (I-III to IV). No association was found between the frequency of oncogene amplification and the tumor phenotype.


CONCLUSIONS
These results suggest that the C-MYC gains are significant for the larynx tumor progression.
Koynova
Denitsa Krasimirova
DK
Department of Medical Genetics, Medical University, Sofia, Bulgaria.
Tsenova
Vesselina Sainova
VS
Toncheva
Draga Ivanova
DI
D002277:Carcinoma / Q000235:genetics* / Q000473:pathology*; D018628:Gene Dosage; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes / Q000235:genetics