title: Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures.
abstract: Normal root cultures of Capsicum frutescens biotransform externally fed precursors, like caffeic acid and veratraldehyde, to vanillin and other related metabolites. The bioconversion of caffeic acid to further metabolites--viz. vanillin, vanillylamine, vanillic acid--was shown to be elicited by treating the cultures with 10 microM methyl jasmonate (MJ). Root cultures treated with MJ accumulated (1.93 times) more of vanillin (20.2 microM on day-3) than untreated ones. A concomitant increase in enzymatic activity of caffeic acid O-methyl transferase (CAOMT, EC 2.1.1.68) was obtained in MJ treated cultures, compared to untreated cultures. After 24 h of MJ treatment, a 13.7-fold increase in CAOMT activity was recorded in root cultures of C. frutescens. Cultures treated with veratraldehyde accumulated more vanillin (78 microM) than caffeic acid fed cultures, 6 days after precursor addition. Capsaicin did not accumulate even after addition of precursors. The efficiencies of biotransformation with caffeic acid and veratraldehyde were 2.2% and 9% with respect to vanillin formation, indicating a possible diversion of the phenylpropanoid pathway towards other secondary metabolites.
authors.affiliation: Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: B
authors.initials: B
authors.lastname: Suresh
authors.forename: G A
authors.initials: GA
authors.lastname: Ravishankar
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D001547:Benzaldehydes / Q000378:metabolism*; D001711:Biotransformation; D002109:Caffeic Acids / Q000378:metabolism*; D002211:Capsaicin / Q000378:metabolism; D002212:Capsicum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology*; D008780:Methyltransferases / Q000378:metabolism; D054883:Oxylipins; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D000085:Acetates; D001547:Benzaldehydes; D002109:Caffeic Acids; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; C100058:vanillin; D008780:Methyltransferases; C019608:caffeate O-methyltransferase; D002211:Capsaicin; C040048:caffeic acid; C427725:veratraldehyde