title: A simple method of DNA extraction in solving difficult criminal cases.
abstract: OBJECTIVE
  We examined combined use in house method and DNA IQ system (Promega) procedure for the extraction DNA from biological trace in order to help the solving some crime cases.
  
  
  METHODS
  Simple and efficient method for extraction of the DNA from biological trace samples included the well washed trousers and the knife without any visual biological trails (from some one hard criminal case). In this method there were used: centrifuge, microwave oven, shaker, chelex, proteinase K, DNA IQ System (Promega).
  
  
  RESULTS
  The extraction of DNA could be done within 2.5 hours or over the night. The DNA prepared on this way was good quality and could be used for STR analysis.
  
  
  CONCLUSIONS
  This combined, simple, and safe method could be used for extraction DNA from samples containing a minute amount of the biological trace.
authors.affiliation: Department for molecular pathology Tuzla, Policlinic for microbiology pathology immunology and molecular medicine, University Clinical Centre of Tuzla, Bosnia and Herzegovina.
authors.forename: Izet
authors.initials: I
authors.lastname: Eminovic
authors.forename: Jasenko
authors.initials: J
authors.lastname: Karamehić
authors.forename: Faris
authors.initials: F
authors.lastname: Gavrankapetanović
authors.forename: Bećir
authors.initials: B
authors.lastname: Heljić
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA