Expression of mdr49 and mdr65 multidrug resistance genes in larval tissues of Drosophila melanogaster under normal and stress conditions.
In situ expression of 2 multidrug resistance genes, mdr49 and mdr65, of Drosophila melanogaster was examined in wild-type third instar larval tissues under physiological conditions and after heat shock or colchicine feeding. Expression of these 2 genes was also examined in tumorous tissues of lethal (2) giant larvae I(2)gl4 mutant larvae. These 2 mdr genes show similar constitutive expression in different larval tissues under physiological conditions. However, they are induced differentially by endogenous (tumorous growth) and exogenous stresses (colchcine feeding or heat shock): whereas heat shock and colchicine feeding induce mdr49, tumorous condition is accompanied by enhanced expression of mdr49 and mdr65 genes.
Tapadia
Madhu G
MG
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India. madhu@banaras.ernet.in
Lakhotia
S C
SC
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019450:Genes, MDR; D006358:Hot Temperature; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva / Q000166:cytology / Q000378:metabolism*; D009154:Mutation; D014018:Tissue Distribution
D018435:ATP Binding Cassette Transporter, Subfamily B; D029721:Drosophila Proteins; C069936:mdr65 protein, Drosophila; C069935:mdr49 protein, Drosophila