title: Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.
abstract: Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: Kacper
authors.initials: K
authors.lastname: Kazimierczuk
authors.forename: Lucyna
authors.initials: L
authors.lastname: Cova
authors.forename: Benedicte
authors.initials: B
authors.lastname: Ndeboko
authors.forename: Urszula
authors.initials: U
authors.lastname: Szczyrk
authors.forename: Aneta
authors.initials: A
authors.lastname: Targosz
authors.forename: Tomasz
authors.initials: T
authors.lastname: Brzozowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sirko
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D004372:Ducks / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D014510:Urease / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; C016512:IgY; D007136:Immunoglobulins; D011994:Recombinant Proteins; D014510:Urease