Characteristic tissue interaction of the diastema region in mice.
Rodents have a toothless diastema between the incisor and the first molar, which may contain rudimentary tooth germs. In the lower diastema region of mice at E13, the rudimentary tooth germs, which developed into the bud stage before its removal by apoptosis, was found. The immunoreactivity to tenascin was observed in the condensed mesenchyme around the normal tooth bud and was detected in only the basement membrane in the diastema bud. This result shows that the relationship between mesenchymal condensation and tooth development. The similar patterns of Msx-1 and Msx-2 expression between the tooth bud and the diastema bud show that the diastema bud may have some other genetic mechanism in the developmental arrest of the rudimentary tooth germs rather than the Msx-1 and Msx-2 expression. Strikingly, the induction of the tooth formation was possible using tissue recombination between the oral epithelium of the diastema bud and the dental mesenchyme of the molar tooth bud, which indicates the potential capability of the diastema in the tooth formation. In conclusion, it is suggested that the condensed mesenchyme may be the key to tooth development.
Yamamoto
Hitoshi
H
Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Oral Science Center, College of Dentistry, Yonsei University, Seoul, Republic of China.
Cho
Sung-Won
SW
Song
Soo-Jin
SJ
Hwang
Heui-Jung
HJ
Lee
Min-Jung
MJ
Kim
Jae-Young
JY
Jung
Han-Sung
HS
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics; D003970:Diastema / Q000196:embryology*; D018398:Homeodomain Proteins / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D007180:Incisor; D051957:MSX1 Transcription Factor; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D008963:Molar; D009805:Odontogenesis / Q000502:physiology*; D019063:Tenascin; D046509:Tissue Culture Techniques; D014083:Tooth Germ / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; D019063:Tenascin; D014157:Transcription Factors