title: Fluorescence probing of albumin-surfactant interaction.
abstract: Protein-surfactant interactions were studied using bovine serum albumin (BSA) and the three surfactants sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB), and poly(oxyethylene)isooctyl phenyl ether (TX-100). The surfactants used belong to three broad classes, i.e., anionic, cationic, and nonionic. These categories of surfactants were used to elucidate the mechanism of surfactant binding to BSA, at pH 7. The interactions were followed fluorimetrically using both intrinsic tryptophan (Trp) fluorescence and the fluorescence of an external label. The aggregation behavior of the surfactants were studied in the presence of BSA. Steady-state fluorescence studies indicate that all three surfactants bind to BSA in a cooperative manner. This cooperative binding affects the binding of the external label to BSA. All these effects are also manifested in time-resolved fluorescence studies. The effects of surfactants on acrylamide quenching and energy transfer from Trp in BSA to bound dye provided valuable insights into the structural modification of BSA in presence of surfactants. The surfactant-induced conformational change of BSA was also confirmed by circular dichroism studies. However, among the three categories of surfactants, the nonionic surfactant shows the least interaction with BSA.
authors.affiliation: Department of Chemistry, University of Kalyani, Kalyani 741235, India. swati@klyuniv.ernet.in
authors.forename: Swati
authors.initials: S
authors.lastname: De
authors.forename: Agnishwar
authors.initials: A
authors.lastname: Girigoswami
authors.forename: Susmita
authors.initials: S
authors.lastname: Das
mesh_terms: D000418:Albumins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D013501:Surface-Active Agents