title: Regional distribution of fluoro-jade B staining in the hippocampus following traumatic brain injury.
abstract: Fluoro-Jade B (FJB) is an anionic fluorescein derivative that has been reported to specifically stain degenerating neurons. We were interested in applying FJB staining in a well-characterized model of traumatic brain injury (TBI) in order to estimate the total number of neurons in different regions of the hippocampus that die after a mild or moderate injury. Rats were subjected to a mild or moderate unilateral cortical contusion (1.0- or 1.5-mm displacement from the cortical surface) with a controlled cortical impacting device. Animals were allowed to survive for 1, 2, or 7 days and the total number of FJB-positive neurons in hippocampal areas CA1, CA3, and the dentate gyrus granule layer was estimated using sterological methods. The region that had the highest number of FJP-positive neurons after TBI was the dentate gyrus. In both 1- and 1.5-mm injuries, FJB-positive granule cells were observed throughout the rostro-caudal extent of the dentate. In contrast, labeled pyramidal neurons of area CA3 were most numerous after the 1.5-mm injury. The area that had the fewest number of FJB-labeled cells was area CA1 with only scattered neurons seen in the 1.5-mm group. In both injury groups and in all hippocampal regions, more FJB-positive neurons were seen at the earlier times post injury (1 and 2 days) than at 7 days. FJB appears to be a reliable marker for neuronal vulnerability following TBI.
authors.affiliation: Sanders-Brown Center on Aging and Department of Anatomy and Neurobiology, University of Kentucky, Lexington, KY 40536, USA. kanders@ufbi.ufl.edu
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Anderson
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Miller
authors.forename: Isabella
authors.initials: I
authors.lastname: Fugaccia
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Scheff
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade