Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells.
Fluorine compounds are widely used for the prevention of caries, and recently sodium fluorosilicate has been used in water fluorination. The cytotoxic effects of sodium fluorosilicate in several osteosarcoma and oral cancer cells were evaluated in this study by measurement of inhibition of cell proliferation. Human osteogenic sarcoma (HOS) cells were the most sensitive to sodium fluorosilicate treatment. Induction of apoptosis, such as nucleosomal DNA fragmentation and the appearance of apoptotic bodies, were observed in HOS cells by agarose gel electrophoresis and by flow cytometric analysis, respectively. The molecular mechanism of apoptosis induction in HOS was investigated by Western blot analysis. The level of Bcl-2 was decreased and consequent release of cytochrome c was increased. Caspase-3 was activated and the cleavage of poly (ADP-ribosyl) polymerase was increased. In conclusion, sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to the cytosol and activation of caspase-3.
Song
Je Seon
JS
Institute of Oral Biology, Department of Biochemistry College of Dentistry, Kyung Hee University, Seoul 130-701, Korea.
Park
Yong Duk
YD
Hyun
Jin Won
JW
Kim
Jeong Hee
JH
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D012516:Osteosarcoma / Q000188:drug therapy* / Q000473:pathology; D012824:Silicic Acid / Q000494:pharmacology*
D012824:Silicic Acid; C004832:hexafluorosilicate; D005459:Fluorides