title: Alternative splicing of the human Kank gene produces two types of Kank protein.
abstract: The human Kank gene encodes an ankyrin repeat domain-containing protein which regulates actin polymerization. There are at least two types of Kank protein depending on cell type, likely due to differences in transcription. Here, to examine the transcriptional initiation and genomic organization of the human Kank gene, we performed 5'-RACE (rapid amplification of cDNA ends) using total RNA from normal kidney and a human kidney cancer cell line, VMRC-RCW cells. The results suggest that the human Kank gene has several alternative first exons. While mRNA from VMRC-RCW cells encoded Kank protein (referred to as Kank-S) as reported previously, mRNA from the normal kidney tissue encoded a novel type of Kank protein (referred to as Kank-L), which contained an additional N-terminal sequence 158 amino acids long. Promoter activity and the expression of the Kank variants in normal and cancer tissues were examined.
authors.affiliation: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Onishi
authors.forename: Naoto
authors.initials: N
authors.lastname: Kakinuma
authors.forename: Badal Chandra
authors.initials: BC
authors.lastname: Roy
authors.forename: Teiichiro
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D002460:Cell Line; D045744:Cell Line, Tumor; D019295:Computational Biology; D003598:Cytoskeletal Proteins; D005091:Exons; D017930:Genes, Reporter; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C467739:KANK1 protein, human; D025521:Tumor Suppressor Proteins; D008156:Luciferases