title: Coronin 3 and its role in murine brain morphogenesis.
abstract: Coronins belong to the fundamental WD40-repeat proteins. They are mainly found at the submembraneous area, they bind F-actin in vitro, and most of the seven mammalian coronins have unclear roles. Coronin 3 is abundantly expressed in the adult CNS. All murine brain areas express coronin 3 during embryogenesis and the first postnatal stages. Expression in grey matter decreases postnatally, except for hippocampal pyramidal and dentate gyrus neurons, and cerebellar Purkinje cells, while levels in white matter increase in the course of myelination. Consistently, coronin 3 is abundant in differentiating neuro-2a and PC-12 cells and in primary oligodendrocytes. Treatment with PKC activator PMA reduced coronin 3 protein levels. To address its functions, neuro-2a and PC-12 cells were transfected with GFP-tagged coronin 3 versions. Full-length coronin 3 among other areas localized to outgrowing neurites, whereas truncated proteins efficiently suppressed neurite formation. Our results favour a role for coronin 3 in neuron morphogenesis and possibly migration.
authors.affiliation: Center for Biochemistry and Center of Molecular Medicine Cologne, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, D-50931 Koeln, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hasse
authors.forename: André
authors.initials: A
authors.lastname: Rosentreter
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Spoerl
authors.forename: Maria
authors.initials: M
authors.lastname: Stumpf
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
authors.forename: Christoph S
authors.initials: CS
authors.lastname: Clemen
mesh_terms: D000199:Actins / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000502:physiology*; D004676:Myelin Basic Protein / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D000199:Actins; D007211:Indoles; D008840:Microfilament Proteins; D004676:Myelin Basic Protein; C485184:enhanced green fluorescent protein; C072061:coronin proteins; D049452:Green Fluorescent Proteins; D015240:Phorbol 12,13-Dibutyrate; C007293:DAPI