title: On-line monitoring of bioreductions via membrane introduction mass spectrometry.
abstract: Real-time and on-line continuous monitoring of reactants, intermediates, and final products for dicarbonyl compound bioreduction in a continuous plug flow reactor packed with baker's yeast (Saccharomyces cerevisiae) whole cells immobilized on calcium alginate beads was performed by membrane introduction mass spectrometry (MIMS) via selective ion monitoring.
authors.affiliation: LABIOSIN, Instituto de Química, Universidade Estadual de Campinas CP 6154, CEP 13084-971 Campinas-SP, Brasil.
authors.forename: Cíntia D F
authors.initials: CD
authors.lastname: Milagre
authors.forename: Humberto M S
authors.initials: HM
authors.lastname: Milagre
authors.forename: J Augusto R
authors.initials: JA
authors.lastname: Rodrigues
authors.forename: Lilian L
authors.initials: LL
authors.lastname: Rocha
authors.forename: Leonardo S
authors.initials: LS
authors.lastname: Santos
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberlin
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods*; D003199:Computer Systems; D017022:Flow Injection Analysis / Q000379:methods; D007659:Ketones / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D008567:Membranes, Artificial; D009862:Online Systems; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D007659:Ketones; D008567:Membranes, Artificial; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase