Imaging magnetically labeled cells with magnetomotive optical coherence tomography.
We introduce a novel contrast mechanism for optical coherence tomography (OCT) whereby the optical scattering of magnetically labeled cells is modified by means of an externally applied magnetic field. This modification is made through the addition of a small electromagnet to the imaging arm of a conventional OCT interferometer. We measure the magnetomotive OCT signal by differencing pairs of axial scans (A-scans) acquired with the magnetic field on and off. Magnetomotive contrast is demonstrated in bulk three-dimensional cell scaffolds containing macrophages labeled with microparticles of iron oxide, demonstrating magnetic-specific contrast over a dynamic range of 30 dB.
Oldenburg
Amy L
AL
Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.
Gunther
Jillian R
JR
Boppart
Stephen A
SA
D000818:Animals; D002460:Cell Line; D003287:Contrast Media; D052203:Ferrosoferric Oxide; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007501:Iron; D008264:Macrophages / Q000166:cytology*; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010087:Oxides; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
D003287:Contrast Media; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide