title: Effect of biofilm model, mode of growth, and strain on streptococcusmutans protein expression as determined by two-dimensional difference gel electrophoresis.
abstract: The effect of biofilm model, strain and mode of growth (biofilm or planktonic) on protein expression in Streptococcus mutans, a dental pathogen, was determined by two-dimensional difference gel electrophoresis. The bacterial strain (21-28% differentially expressed proteins) and the biofilm model (0.3-7.8% differential expression) used have a much larger effect on protein expression than the mode of growth (0.2-0.7% differential expression), something that has been ignored in biofilm studies up to now.
authors.affiliation: Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, Louwesweg 1, 1066 EA Amsterdam, The Netherlands. s.luppens@acta.nl
authors.forename: Suzanne B I
authors.initials: SB
authors.lastname: Luppens
authors.forename: Jacob M
authors.initials: JM
authors.lastname: ten Cate
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008954:Models, Biological; D013295:Streptococcus mutans / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins