Soluble guanylyl cyclase appears in a specific subset of periglomerular cells in the olfactory bulb.
In the brain, nitric oxide acts as an atypical messenger in cellular nonsynaptic transmission. In the olfactory bulb, this gas is produced at the level of the olfactory glomeruli by a subpopulation of periglomerular cells that participates in the first synaptic relay of the olfactory information between the olfactory nerve and the dendritic tufts of principal cells. It has been proposed that nitric oxide modulates intraglomerular synaptic integration of sensory inputs, but its specific role in the glomerular circuitry remains to be understood. In this article, we demonstrate that, in the glomerular circuits, a specific subset of periglomerular cells, most of them expressing the calcium binding protein calbindin D-28 k, expresses the beta1 subunit of the soluble guanylyl cyclase. These cells could be the targets for the action of nitric oxide at the glomerular level via activation of soluble guanylyl cyclase and production of cGMP.
Gutièrrez-Mecinas
Maria
M
Departamento de Biología Celular, Facultad de Ciencias Biológicas, Universidad de Valencia, Street Dr Moliner, 50 E-46100, Burjasot, Spain.
Crespo
Carlos
C
Blasco-Ibáñez
José Miguel
JM
Gracia-Llanes
Francisco Javier
FJ
Marqués-Marí
Ana Isabel
AI
Martínez-Guijarro
Francisco José
FJ
D000818:Animals; D064032:Calbindin 2; D064026:Calbindins; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000379:methods; D009474:Neurons / Q000145:classification / Q000201:enzymology* / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D009833:Olfactory Pathways / Q000201:enzymology; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D064032:Calbindin 2; D064026:Calbindins; D020033:Protein Isoforms; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase; D006162:Guanylate Cyclase