First preparation of biotinylated gradient polyethylene surface to bind photoactive caged streptavidin.
A gradient polyethylene (PE) surface was created through corona treatment, in which the corona power increased along the 5 cm length of the PE. The gradient surface was treated with polyethyleneimine and then biotin. Fluorescein-conjugated streptavidin (SAV) caged within 5-carboxymethoxy-2-nitrobenzyl (CMNB) adsorbed onto the biotinylated gradient PE surface following molecular recognition principles. Photoirradiation decomposed the CMNB cage and allowed the fluorescein-conjugated SAV to fluoresce, the intensity of which increased gradually along the PE surface.
Kim
Moon Suk
MS
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea.
Seo
Kwang Su
KS
Khang
Gilson
G
Lee
Hai Bang
HB
D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D009578:Nitrobenzenes / Q000737:chemistry; D010777:Photochemistry; D020959:Polyethylene / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
D005452:Fluoresceins; D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D001710:Biotin; D020959:Polyethylene; D011094:Polyethyleneimine; D019809:Streptavidin