Rapid protocol for electroporation of Clostridium perfringens.
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.
Jirásková
Alena
A
Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.
Vítek
Libor
L
Fevery
Johan
J
Ruml
Tomás
T
Branny
Pavel
P
D003016:Clostridium perfringens / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*