Effect of penicillin on the in vivo formation of the D-alanyl-L-alanine peptide cross-linkage in cell walls of Micrococcus luteus.
Penicillin G was found to inhibit the formation of the d-alanyl-l-alanine cross-linkage in intact cells of Micrococcus luteus. This reaction was approximately 50-fold less susceptible to penicillin than the formation of the d-alanyl-l-lysine cross-linkage in the same organism. The presence of two penicillin-susceptible transpeptidation reactions that function in the incorporation of peptidoglycan precursors into the cell wall is proposed.
Mirelman
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D
Bracha
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R
D000409:Alanine / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000187:drug effects* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D016982:Micrococcus luteus / Q000187:drug effects* / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D010406:Penicillins; D008239:Lysine; D000409:Alanine