In vitro study of alginate-chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failure.
The application of alginate-chitosan (AC) microcapsules to liver cell transplantation has not been previously investigated. In the current in vitro study, we have investigated the potential of AC microcapsules for the encapsulation of liver cells and show that the AC membrane supports the survival, proliferation and protein secretion by entrapped hepatocytes. The AC membrane provides cell immuno-isolation and has the potential for cell cryopreservation. The AC microcapsule has several advantages compared to more widely used alginate-poly-L-lysine (APA) microcapsules for the application of cell therapy.
Haque
Tasima
T
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Organs Research Center, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada H3A 2B4.
Chen
Hongmei
H
Ouyang
Wei
W
Martoni
Christopher
C
Lawuyi
Bisi
B
Urbanska
Aleksandra Malgorzata
AM
Prakash
Satya
S
D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials; D001709:Biotechnology / Q000379:methods*; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D017690:Cell Transplantation; D048271:Chitosan / Q000737:chemistry*; D015925:Cryopreservation; D004337:Drug Carriers; D020723:Glucuronic Acid / Q000737:chemistry; D022781:Hepatocytes / Q000473:pathology; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000473:pathology*; D017093:Liver Failure / Q000628:therapy*; D008863:Microspheres; D011107:Polylysine / Q000737:chemistry; D013997:Time Factors
D000464:Alginates; D001672:Biocompatible Materials; D004337:Drug Carriers; D006603:Hexuronic Acids; D011107:Polylysine; D020723:Glucuronic Acid; D048271:Chitosan