[Characteristic of vibration spectra for hydration state of herring sperm DNA fibers].
By different temperature treatment, the herring sperm DNA fibers at various relative humidity have been analyzed using Raman scattering spectroscopic method for estimation of bound water content in fiber according to the typical peak intensity ratio between deoxyribosyl C-H stretching vibration (at 2964cm(-1)) and bound water O-H stretching vibration (at 3204cm(-1)). We found the bound water content of the fibers to be 9-14 water molecules per nucleotide pair (i.e. base pair) at variance with the calculated value of calf thymus DNA fiber by Tao's Raman test formula, but in similarity to the determined value by IR spectroscopy, X-ray diffraction, BET gravimetric analysis and differential scanning calorimetry analysis. Thus, herring sperm DNA fibers should have the same hydration state as both calf thymus DNA and salmon sperm DNA, that has been confirmed with the new formula: 2.5926 x I2964/I3204.
Yu
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Department of Biology, Nanjing Normal University, 210097 Nanjing.
Ke
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W
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D005399:Fishes; D008297:Male; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D013094:Spermatozoa; D014732:Vibration; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
D014867:Water; D004247:DNA