title: Molecular cloning and sequence analysis of the porcine precursor of endothelin-2.
abstract: The amino acid sequences of two of the three endothelin (ET) family peptides, ET-1 and ET-3, are identical among mammals, whereas for the other family member, ET-2 or vasoactive intestinal contractor (VIC), the mouse and rat sequences differ from the human counterpart ET-2 by one amino acid residue. To examine more deeply the structural diversity among ET-2/VIC orthologs (EDN2), we screened porcine ET-2/VIC-like cDNAs using the 5' rapid amplification of cDNA ends (RACE) method with degenerate primers based on ET-2/VIC mature peptides. Sequence analysis of the cDNAs showed that ET-2 is present in pig. The full-length cDNA sequence, produced by combining 5' RACE and 3' RACE products, revealed the porcine precursor protein of ET-2 (PPET-2). Porcine PPET-2, made up of 214 amino acids, includes a 26-residue putative signal sequence, big ET-2, mature ET-2, and ET-2-like peptide. The percent sequence identity of porcine PPET-2 with human PPET-2, and rat or mouse precursor protein of VIC runs between approximately 70% and 74%. ET-2, although expressed in intestine, has no anti-microbial activity.
authors.affiliation: Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Japan. k.saida@aist.go.jp
authors.forename: Kaname
authors.initials: K
authors.lastname: Saida
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uchide
authors.forename: Shaoxuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Javier
authors.initials: J
authors.lastname: Adur
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kawano
authors.forename: Masayo
authors.initials: M
authors.lastname: Ogiso
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Oka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takizawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D016232:Endothelins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D007422:Intestines / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D016232:Endothelins; D011498:Protein Precursors; C099648:preproendothelin 2