[The study on the treatment of Oviducts Ranae protein with enzyme hydrolysis].
The paper introduced a process to enhance the yield of Oviducts Ranae protein using in vitro enzyme hydrolysis. The treatment process included two steps: (1) a 3 - 4 h of hydrolysis of a 0.025 g x g(-1) concentration of substance, at pH 7 and 60 degrees C, using 4% of papain; and (2) followed with a 6 - 8 h hydrolysis, at pH 2 - 2.5 and 60 degrees C, using 3% of pepsin. This treatment process significantly improved the lyophilized Oviducts Ranae in solubility and fluidity, which is convenient for the relative pharmaceutical preparations.
Li
Jin-Ming
JM
Harbin Business University, Harbin 150040, China. sunximi@yeah.net
D029845:Amphibian Proteins / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D004958:Estradiol / Q000032:analysis; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008419:Materia Medica / Q000737:chemistry*; D010057:Oviducts / Q000737:chemistry*; D010206:Papain / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D011893:Rana esculenta
D029845:Amphibian Proteins; D008419:Materia Medica; D004958:Estradiol; D010206:Papain; D010434:Pepsin A