title: [Culturing and cryopreservation of pre-cartilaginous stem cells from neonate rat].
abstract: OBJECTIVE
  To establish an effective way to cryopreserve pre-cartilaginous stem cells (PSCs) of neonate rat.
  
  
  METHODS
  PSCs [fibroblast growth factor-3 (FGFR-3) positive cells] were isolated and purified by magnetic cell sorting method. PSCs were cultured and amplified to the third generation. PSCs were preserved in liquid nitrogen. The biological properties of cryopreserved PSCs were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), immunohistochemistry, and immunofluorescence.
  
  
  RESULTS
  Immunohistochemical and immunofluorescent analysis showed widespread expression of FGFR-3 in cryopreserved PSCs. FGFR-3 could be detected by RT-PCR in cryopreserved PSCs. Cryopreserved PSCs kept high cell viability, and phenotypic and proliferation characteristics of PSCs in vivo.
  
  
  CONCLUSIONS
  Cryopreservation of PSCs can supply adequate qualified cells for repairing the defects of epiphyseal growth plate by tissue engineering technique.
authors.affiliation: Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei 430030, PR China. youhongbo001@sina.com
authors.forename: Hongbo
authors.initials: H
authors.lastname: You
authors.forename: Anmin
authors.initials: A
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D015925:Cryopreservation; D004838:Epiphyses / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D051498:Receptor, Fibroblast Growth Factor, Type 3