title: Acute stress response in calorie-restricted rats to lipopolysaccharide-induced inflammation.
abstract: Calorie restriction (CR) reduces morbidity and mortality in a wide range of organisms, possibly through the stress response machinery. We analyzed the acute phase response of CR rats to lipopolysaccharide (LPS)-induced inflammatory challenge. Six-month-old male F344 rats, fed ad libitum (AL) or a 30% calorie-restricted diet from 6 weeks of age, received an intravenous LPS injection and were then sacrificed between 0 and 8 h. CR attenuated liver injury without reduction in the plasma concentrations of proinflammatory cytokines or nitric oxide (NO). Western blotting analysis of liver tissue demonstrated that CR did not affect the degradation of cytoplasmic I-kappaB and subsequent nuclear translocation of NF-kappaB, a key transcription factor after inflammatory challenge. We also analyzed the liver gene expression profiles at 0, 1 and 4 h with DNA arrays and cluster analysis. Compared with the AL group, CR upregulated the expression of several genes for inflammatory mediators or their related molecules at 0 h, but not at 1 or 4 h. CR downregulated genes for energy or xenobiotic metabolism and stress response proteins at 0 h. At 1 h, the relatively downregulated genes by CR were those for proteases and the ubiquitin-proteasome pathway. The present results suggest that CR attenuates liver injury without suppression of the proinflammatory response, and that the protective effect emerges from constitutively, rather than inductively, expressed gene products.
authors.affiliation: Department of Pathology and Gerontology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki City 852-8523, Japan.
authors.forename: Tomoshi
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Komatsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Sumihisa
authors.initials: S
authors.lastname: Honda
authors.forename: Haruyoshi
authors.initials: H
authors.lastname: Yamaza
authors.forename: Takuya
authors.initials: T
authors.lastname: Chiba
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ayabe
authors.forename: Isao
authors.initials: I
authors.lastname: Shimokawa
mesh_terms: D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D031204:Caloric Restriction; D002467:Cell Nucleus / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D003593:Cytoplasm / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced* / Q000150:complications*; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism; D008297:Male; D005810:Multigene Family; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011916:Rats, Inbred F344; D013312:Stress, Physiological / Q000209:etiology*
chemical_list: D016207:Cytokines; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; D000410:Alanine Transaminase