[Effect of indomethacin on the growth and invasion of Hep-2 cells in human laryngeal cancer].
OBJECTIVE
To assess the effect of indomethacin on the growth and invasion of Hep-2 cell line in human laryngeal cancer in vitro.


METHODS
Hep-2 cell line was exposed to indomethacin at different concentration for 24 and 48 h. Then the cells were counted by trypan blue exclusion test for determining the viability of cells. And the cell DNA analysis was conducted with flow cytometry. Cell morphology was observed by phase contrast microscope and transmission electron microscope. Hep-2 cells were exposed to indomethacin at different concentration for 48 h. Then the cell growth rate, the colonies formation in soft agar medium and cell mobility were examined. Monolayer invasion assay gave us cell invasion index.


RESULTS
The alive cells after indomethacin treatment were reduced to a dose and time-dependent situation. And the cells appeared a significant peak of apoptosis, in flow cytometry analysis. Hep-2 cells were smaller and longer than in control; dark cells also can be seen. Dealed with indomethacin, the colonies formed of Hep-2 cells were fewer than that of control; the cell mobility were weakened and the invasion index were decreased,but cells growth rate weren't changed.


CONCLUSIONS
Indomethacin could inhibit the growth and invasion of Hep-2 cells in vitro.
Gao
Yu
Y
Department of Otolaryngology, the First Affiliated Hospital of Jinan University, Guangzhou, 510632, China.
Wang
Jiqun
J
Shan
Yanchun
Y
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000473:pathology*
D007213:Indomethacin