title: Rapid immunofluorescence microscopy for diagnosis of melioidosis.
abstract: An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.
authors.affiliation: Faculty of Tropical Medicine, Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program, Bangkok, Thailand. lek@tropmedres.ac
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Varunee
authors.initials: V
authors.lastname: Desakorn
authors.forename: Gumphol
authors.initials: G
authors.lastname: Wongsuvan
authors.forename: Premjit
authors.initials: P
authors.lastname: Amornchai
authors.forename: Allen C
authors.initials: AC
authors.lastname: Cheng
authors.forename: Bina
authors.initials: B
authors.lastname: Maharjan
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Chierakul
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000942:Antigens, Bacterial / Q000097:blood*; D001431:Bacteriological Techniques; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial