SCAPE: a new tool for the Selective CApture of PEptides in protein identification.
A method for the selective capture and analysis of peptides containing neither histidine nor arginine is evaluated. It is based on the reversible modification of alpha- and epsilon-amino groups of peptides and the relatively easy separation of charged and noncharged peptides by cation exchange chromatography. The simplicity of the method and the results obtained in silico and with standard proteins, anticipate the utility of the SCAPE approach for proteome analyses.
Betancourt
Lazaro
L
Department for Proteome Analysis, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba. lazaro.betancourt@cigb.edu.cu
Gil
Jeovanis
J
Besada
Vladimir
V
González
Luis J
LJ
Fernández-de-Cossio
Jorge
J
García
Liudy
L
Pajón
Rolando
R
Sanchez
Aniel
A
Alvarez
Félix
F
Padrón
Gabriel
G
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D010455:Peptides; D011506:Proteins