A linearization method for low catalytic activity enzyme kinetic analysis.
A kinetic analysis was made and a linear plot based on the general rate equation derived by Laidler [Can. J. Chem. 33, 1614-1624] is proposed. This linearization method allows determining the kinetic parameters (K(m), k(cat)) and [E](0) for enzymes with low catalytic activity. The method was applied to chloroperoxidase from Caldariomyces fumago [EC 1.11.1.10], whose kinetic parameters K(m)(app), k(cat)(app), and [E](0) with monochlorodimedone as substrate, were obtained by using the linearization plot and the V(max) value (calculated by Eadie-Hofstee plot). This plot could also be useful to the study of abenzyme kinetics provided the concentration of the latter is either higher or equal than K(m) value.
Toti
Paolo
P
Department of Physiology and Biochemistry, Biochemistry Unit, University of Pisa, Italy.
Petri
Antonella
A
Pelaia
Valerio
V
Osman
Ahmed M
AM
Paolini
Moreno
M
Bauer
Carlo
C
D002384:Catalysis; D002711:Chloride Peroxidase / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology; D007700:Kinetics; D016014:Linear Models
D002711:Chloride Peroxidase