title: Proteins released from liver after ischaemia induced an elevation of heart resistance against ischaemia-reperfusion injury: 2. Beneficial effect of liver ischaemia in situ.
abstract: We have shown earlier that proteins released from the heart during preconditioning may protect non-preconditioned heart during sustained ischaemia, similarly as preconditioning itself. In other our experiments we have documented that also proteins released from isolated rat liver during reperfusion after global ischaemia performed a protective effect on isolated rat heart against ischaemia-reperfusion injury. In the current study we examined the effect of liver ischaemia in situ on resistance of rat heart to ischaemia and reperfusion injury. Wistar rats (male) were subjected to liver ischaemia maintained by occlusion of portal vein and hepatic artery for 20 min, followed with 30-min reperfusion after reopening of both vessels. Then the hearts were isolated and perfused according to Langendorf. Hearts, after initial stabilisation (15 min), were subjected to 20-min ischaemia and 30-min reperfusion. During reperfusion, the haemodynamic parameters of hearts were measured. The protein pattern of high soluble fraction (HS fraction) isolated from rat blood by precipitation with ammonium sulphate was detected by SDS-PAGE. Our results showed improved parameters of pressure and contractility in the group after liver ischaemia (ischaemic group), presented by decreased diastolic pressure and increased LVDP((S-D)) in comparison with levels of these parameters in the control group. We also observed improved heart contraction-relaxation cycles parameters (dP/dt)(max) and (dP/dt)(min) in ischaemic group as compared with the control group. On the other hand, there were no significant differences in heart rate and coronary flow between both experimental groups. SDS-PAGE showed changed protein pattern in HS fraction, particularly the levels of several low molecular weight proteins increased. We conclude that liver ischaemia induced a higher resistance of heart against ischaemia-reperfusion injury. We propose that release of some cardioprotective proteins present in HS fraction can also contribute to this cardioprotection.
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Barteková
authors.forename: Z
authors.initials: Z
authors.lastname: Sulová
authors.forename: D
authors.initials: D
authors.lastname: Pancza
authors.forename: T
authors.initials: T
authors.lastname: Ravingerová
authors.forename: T
authors.initials: T
authors.lastname: Stankovicová
authors.forename: J
authors.initials: J
authors.lastname: Styk
authors.forename: A
authors.initials: A
authors.lastname: Breier
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D007113:Immunity, Innate; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000175:diagnosis* / Q000188:drug therapy* / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D002316:Cardiotonic Agents; D011506:Proteins