Expression of a bioactive recombinant human interleukin-11 in chicken HD11 cell line.
To direct the synthesis and secretion of recombinant human interleukin-11 (rhIL-11) in chicken HD11 cells, a plasmid targeting the c-lysozyme gene has been constructed which contains the mature cytokine cDNA in frame with the lysozyme leader sequence. The upregulation of rhIL-11 mediated by LPS proves the knock-in of hIL-11 cDNA in the lysozyme gene. The bioactivity of the expressed protein is demonstrated and quantified with the hIL-11 dependent 7TD1 and B9 cell lines. The electrophoretic mobility, receptor binding properties and growth promoting effect of the chicken-derived cytokine are identical to those of a rhIL-11 expressed in Escherichia coli. These results describe the secretion of a biologically active rhIL-11 expressed by an avian cellular machinery.
Léon
Arnaud
A
Vivalis SA, CHU Hôtel Dieu, Place A. Ricordeau, 1er étage HNB Aile Nord, 44093 Nantes Cedex 01, France.
Wang
Xiao-Ming
XM
Champion-Arnaud
Patrick
P
Sobczyk
André
A
Pain
Bertrand
B
Content
Jean
J
Jacques
Yannick
Y
Valarché
Isabelle
I
D020121:5' Untranslated Regions / Q000737:chemistry; D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation; D002645:Chickens; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism; D008957:Models, Genetic; D009113:Muramidase / Q000737:chemistry / Q000235:genetics; D010957:Plasmids / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry*; D011995:Recombination, Genetic; D014162:Transfection; D015854:Up-Regulation
D020121:5' Untranslated Regions; D016207:Cytokines; D018076:DNA, Complementary; D017370:Interleukin-11; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D009113:Muramidase