title: Identification of serum proteins related to adverse effects induced by docetaxel infusion from protein expression profiles of serum using SELDI ProteinChip system.
abstract: BACKGROUND
  For the development of quick and easy methods for screening and identifying treatment-responsive proteins, we determined the protein expression profile of the serum after docetaxel infusion using a surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI TOF-MS) system.
  
  
  METHODS
  Blood from breast cancer patients was collected before and 4, 8, 24 and 48 hours after docetaxel infusion. The protein expression profile was determined by a SELDI TOF-MS system. The relative expression levels of target proteins were compared during the time-course after docetaxel injection.
  
  
  RESULTS
  We identified two representative proteins with molecular weights of 7790 Da and 9285 Da. The 7790 Da protein was high molecular weight kininogen, and the 9285 Da protein was apolipoprotein A-II. These two proteins had similar expression patterns in 5 patients, except one patient who experienced severe, acute, adverse effects.
  
  
  CONCLUSIONS
  These results suggest that protein expression profiles determined by SELDI TOF-MS represent useful data for the identification of treatment-responsive proteins.
authors.affiliation: Department of Medical Oncology and Clinical Research, National Shikoku Cancer Center, Matsuyama, Ehime 790-0007, Japan. yheike@ncc.go.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Heike
authors.forename: Mami
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Shozo
authors.initials: S
authors.lastname: Osumi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujii
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Aogi
authors.forename: Nagio
authors.initials: N
authors.lastname: Takigawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Ida
authors.forename: Hisao
authors.initials: H
authors.lastname: Tajiri
authors.forename: Kenji
authors.initials: K
authors.lastname: Eguchi
authors.forename: Mieko
authors.initials: M
authors.lastname: Shiwa
authors.forename: Rumi
authors.initials: R
authors.lastname: Wakatabe
authors.forename: Hisashi
authors.initials: H
authors.lastname: Arikuni
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takaue
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Takashima
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000188:drug therapy; D000077143:Docetaxel; D005260:Female; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D043823:Taxoids / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D043823:Taxoids; D000077143:Docetaxel