title: Depolarization induces Rho-Rho kinase-mediated myosin light chain phosphorylation in kidney tubular cells.
abstract: Myosin-based contractility plays important roles in the regulation of epithelial functions, particularly paracellular permeability. However, the triggering factors and the signaling pathways that control epithelial myosin light chain (MLC) phosphorylation have not been elucidated. Herein we show that plasma membrane depolarization provoked by distinct means, including high extracellular K(+), the lipophilic cation tetraphenylphosphonium, or the ionophore nystatin, induced strong diphosphorylation of MLC in kidney epithelial cells. In sharp contrast to smooth muscle, depolarization of epithelial cells did not provoke a Ca(2+) signal, and removal of external Ca(2+) promoted rather than inhibited MLC phosphorylation. Moreover, elevation of intracellular Ca(2+) did not induce significant MLC phosphorylation, and the myosin light chain kinase (MLCK) inhibitor ML-7 did not prevent the depolarization-induced MLC response, suggesting that MLCK is not a regulated element in this process. Instead, the Rho-Rho kinase (ROK) pathway is the key mediator because 1) depolarization stimulated Rho and induced its peripheral translocation, 2) inhibition of Rho by Clostridium difficile toxin B or C3 transferase abolished MLC phosphorylation, and 3) the ROK inhibitor Y-27632 suppressed the effect. Importantly, physiological depolarizing stimuli were able to activate the same pathway: L-alanine, the substrate of the electrogenic Na(+)-alanine cotransporter, stimulated Rho and induced Y-27632-sensitive MLC phosphorylation in a Na(+)-dependent manner. Together, our results define a novel mode of the regulation of MLC phosphorylation in epithelial cells, which is depolarization triggered and Rho-ROK-mediated but Ca(2+) signal independent. This pathway may be a central mechanism whereby electrogenic transmembrane transport processes control myosin phosphorylation and thereby regulate paracellular transport.
authors.affiliation: Department of Surgery, The Toronto General Hospital, University Health Network, Toronto, Ontario, Canada.
authors.forename: Katalin
authors.initials: K
authors.lastname: Szászi
authors.forename: Gábor
authors.initials: G
authors.lastname: Sirokmány
authors.forename: Caterina
authors.initials: C
authors.lastname: Di Ciano-Oliveira
authors.forename: Ori D
authors.initials: OD
authors.lastname: Rotstein
authors.forename: András
authors.initials: A
authors.lastname: Kapus
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004285:Dogs; D004558:Electric Stimulation; D004847:Epithelial Cells / Q000201:enzymology*; D047908:Intracellular Signaling Peptides and Proteins; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000378:metabolism*; D018374:LLC-PK1 Cells; D008564:Membrane Potentials / Q000502:physiology; D018994:Myosin Light Chains / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013552:Swine; D027981:Symporters / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D054460:rho-Associated Kinases
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D047908:Intracellular Signaling Peptides and Proteins; D018994:Myosin Light Chains; D027981:Symporters; C052168:sodium-alanine cotransporter; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; D020741:rho GTP-Binding Proteins; D011188:Potassium; D002118:Calcium