Application of cation-coated filter method to detection of noroviruses, enteroviruses, adenoviruses, and torque teno viruses in the Tamagawa River in Japan.
The occurrence of human enteric viruses in surface water in the Tamagawa River, Japan, was surveyed for 1 year, from April 2003 to March 2004. Sixty-four samples were collected from six sites along the river, and 500 ml of the sample was concentrated using the cation-coated filter method, which was developed in our previous study. This method showed recovery yields of 56% +/- 32% (n = 37) for surface water samples inoculated with polioviruses. More than one kind of tested virus was detected in 43 (67%) of 64 samples by TaqMan PCR. Noroviruses and adenoviruses were detected in a high positive ratio; 34 (53%), 28 (44%), and 29 (45%) of 64 samples were positive for norovirus genotype 1 and genotype 2 and adenoviruses, respectively. The mean concentrations of norovirus genotype 1 or genotype 2 determined by real-time PCR were 0.087 and 0.61 genome/ml, respectively, showing much higher values in winter (0.21 genome/ml for genotype 1 and 2.3 genomes/ml for genotype 2). Enteroviruses were detected by both direct PCR (6 of 64 samples; 9%) and cell culture PCR (2 of 64 samples; 3%). Torque teno viruses, emerging hepatitis viruses, were also isolated in three samples (5%). The concentration of total coliforms and the presence of F-specific phages showed a high correlation with the presence of viruses, which suggested that the simultaneous use of total coliforms and F-specific phages as indicators of surface water may work to monitor viral contamination.
Haramoto
Eiji
E
Department of Urban Engineering, School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan. haramoto@env.t.u-tokyo.ac.jp
Katayama
Hiroyuki
H
Oguma
Kumiko
K
Ohgaki
Shinichiro
S
D000256:Adenoviridae / Q000302:isolation & purification*; D002412:Cations; D004770:Enterovirus / Q000302:isolation & purification*; D005374:Filtration; D029322:Norovirus / Q000302:isolation & purification*; D045483:Rivers / Q000382:microbiology*; D022783:Torque teno virus / Q000302:isolation & purification*; D014881:Water Supply / Q000592:standards*
D002412:Cations