Dissociation of heterochromatin protein 1 from lamin B receptor induced by human polyomavirus agnoprotein: role in nuclear egress of viral particles.
The nuclear envelope is one of the chief obstacles to the translocation of macromolecules that are larger than the diameter of nuclear pores. Heterochromatin protein 1 (HP1) bound to the lamin B receptor (LBR) is thought to contribute to reassembly of the nuclear envelope after cell division. Human polyomavirus agnoprotein (Agno) has been shown to bind to HP1alpha and to induce its dissociation from LBR, resulting in destabilization of the nuclear envelope. Fluorescence recovery after photobleaching showed that Agno increased the lateral mobility of LBR in the inner nuclear membrane. Biochemical and immunofluorescence analyses showed that Agno is targeted to the nuclear envelope and facilitates the nuclear egress of polyomavirus-like particles. These results indicate that dissociation of HP1alpha from LBR and consequent perturbation of the nuclear envelope induced by polyomavirus Agno promote the translocation of virions out of the nucleus.
Okada
Yuki
Y
Laboratory of Molecular and Cellular Pathology, Graduate School of Hokkaido University, N15, W7, Kita-ku, Sapporo 060-8638, Japan.
Suzuki
Tadaki
T
Sunden
Yuji
Y
Orba
Yasuko
Y
Kose
Shingo
S
Imamoto
Naoko
N
Takahashi
Hidehiro
H
Tanaka
Shinya
S
Hall
William W
WW
Nagashima
Kazuo
K
Sawa
Hirofumi
H
D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D007577:JC Virus / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism*; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins; D019065:Virus Assembly; D000097682:Lamin B Receptor; D000086082:Viroporin Proteins
D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone; D018160:Receptors, Cytoplasmic and Nuclear; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D000097682:Lamin B Receptor; C000715594:CBX5 protein, human; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins