title: Perchloric acid-soluble protein regulates cell proliferation and differentiation in the spinal cord of chick embryos.
abstract: The role of perchloric acid-soluble protein (PSP) was investigated in chick embryos. Fluorescently labeled anti-chick liver (CL)-PSP IgG was injected into the yolk sac in ovo at embryonic day 3, and became localized in neuroepithelial cells. Within 12 h, morphological changes were observed in 37.5% of anti-CL-PSP IgG-injected embryos, and the neuroepithelial cells formed a wavy line. No significant changes were observed in embryos injected with non-immune IgG or PBS. Increased expression of PCNA and decreased expression of neuronal class III beta-tubulin were observed in the spinal cord after anti-CL-PSP IgG injection. These results suggest that PSP controls the proliferation and differentiation of neuroepithelial cells in chick embryos.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Himeno
authors.forename: Kunitaka
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kanouchi
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: Tatsuzo
authors.initials: T
authors.lastname: Oka
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D010472:Perchlorates / Q000494:pharmacology*; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D012995:Solubility / Q000187:drug effects; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D010472:Perchlorates; D012260:Ribonucleases