title: Deletion in chromosome 11 and Bcl-1/Cyclin D1 alterations are independently associated with the development of uterine cervical carcinoma.
abstract: OBJECTIVE
  The aim of this study was to understand whether there is any association between specific deleted regions in chromosome 11 (chr.11) and alteration (amplification/rearrangement) of Bcl-1/Cyclin D1 locus, located at 11q13, in uterine cervical carcinoma (CA-CX).
  
  
  METHODS
  The deletion mapping of chr.11 was studied using 17 highly polymorphic microsatellite markers in 65 primary uterine cervical lesions. The Bcl-1/Cyclin D1 alterations were analyzed by Southern blot and/or polymerase chain reaction (PCR) method in respective cervical lesions.
  
  
  RESULTS
  Chr.11 deletion was found to be significantly associated with progression of CA-CX. High frequency (48-65%) of deletion was found in 11p15.5 (D1), 11q22.3-23.1(D2), and 11q23.3-24.1(D3) regions and significant association was seen among deletions in D2 and D3 regions. Bcl-1/Cyclin D1 locus alteration was observed in overall 27% cervical lesions. Co-amplification of Bcl-1/Cyclin D1 locus was seen in 10% samples. However, no association was found between the deleted regions and Bcl-1/Cyclin D1 locus alterations.
  
  
  CONCLUSIONS
  Our study suggests that there is no co-operativity between the deleted regions (D1- D3) in chr.11 and Bcl-1/Cyclin D1 alterations, but these alterations may provide cumulative effect in progression of the tumor. The D1-D3 regions may harbor candidate tumor suppressor gene(s) (TSGs) associated with the development of CA-CX.
authors.affiliation: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37 S.P. Mukherjee Road, 700026, Kolkata, India.
authors.forename: Ratnesh Kumar
authors.initials: RK
authors.lastname: Singh
authors.forename: Santanu
authors.initials: S
authors.lastname: Dasgupta
authors.forename: Nilanjana
authors.initials: N
authors.lastname: Bhattacharya
authors.forename: Neelanjana
authors.initials: N
authors.lastname: Chunder
authors.forename: Ranjit
authors.initials: R
authors.lastname: Mondal
authors.forename: Anup
authors.initials: A
authors.lastname: Roy
authors.forename: Syamsundar
authors.initials: S
authors.lastname: Mandal
authors.forename: Susanta
authors.initials: S
authors.lastname: Roychowdhury
authors.forename: Chinmay Kumar
authors.initials: CK
authors.lastname: Panda
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D019940:Genes, bcl-1 / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012097:Repressor Proteins / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000473:pathology / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; C084762:oncogene protein E1, Human papillomavirus type 18