The O-polysaccharide from the lipopolysaccharide of Providencia stuartii O44 contains L-quinovose, a 6-deoxy sugar rarely occurring in bacterial polysaccharides.
The O-polysaccharide (O-antigen) of Providencia stuartii O44:H4 (strain 3768/51) was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, and H-detected (1)H,(13)C HSQC, and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [Formula: see text].
Kocharova
Nina A
NA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Ovchinnikova
Olga G
OG
Toukach
Filip V
FV
Torzewska
Agnieszka
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D002240:Carbohydrate Sequence; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry* / Q000276:immunology
D019081:O Antigens; C504167:O-polysaccharide, Providencia stuartii O44; D011135:Polysaccharides, Bacterial; D003847:Deoxyglucose; C037904:6-deoxyglucose