Babesia caballi and Babesia equi: implications of host sialic acids in erythrocyte infection.
The present study investigated the involvement of host sialic acids in the erythrocyte infection by two equine Babesia parasites, Babesia equi and Babesia caballi. We observed that the in vitro growth of both parasites is influenced by the removal of sialic acids from the surface of equine erythrocytes (RBC). When the parasites were cultured with neuraminidase (Nm, EC 3.2.1.18)-treated RBC, in which alpha2-3-linked sialic acid residues were removed from four membrane proteins of the RBC, B. caballi showed a significant inhibition of the erythrocyte invasion, while the intracellular development of B. equi seemed to be significantly affected. The possible involvement of host sialic acid in the erythrocyte invasion by B. caballi was also supported by a significant reduction in the parasite growth accompanied by an increased number of extracellular merozoites after the addition of exogenous 3'-sialyllactose (Neu5Acalpha(2-3)Galbeta(1-4)Glc) into the culture. These results suggest that the alpha2-3-linked sialic acid residues on host RBC play important roles in the erythrocyte infections by B. caballi and B. equi.
Okamura
Masashi
M
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Hokkaido, Japan.
Yokoyama
Naoaki
N
Wickramathilaka
Nilanthi P A L K
NP
Takabatake
Noriyuki
N
Ikehara
Yuzuru
Y
Igarashi
Ikuo
I
D000818:Animals; D001403:Babesia / Q000254:growth & development / Q000502:physiology*; D001404:Babesiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry / Q000469:parasitology*; D006734:Horse Diseases / Q000097:blood / Q000469:parasitology; D006736:Horses; D009439:Neuraminidase / Q000378:metabolism; D018512:Parasitemia / Q000097:blood / Q000469:parasitology / Q000662:veterinary; D012794:Sialic Acids / Q000378:metabolism / Q000502:physiology*
D012794:Sialic Acids; D009439:Neuraminidase