Enzymatic lithography of phospholipid bilayer films by stereoselective hydrolysis.
The stereoselective phospholipase A2-catalyzed hydrolysis of patterned phospholipid bilayers consisting of the l- and d-isomers of alpha-dilauroylphosphatidylcholine (DLPC) and alpha-dipalmitoylphosphatidylcholine (DPPC) is reported. The stereochemically directed enzyme lithography demonstrated herein allows the parallel modification of large surface areas and constitutes a potentially useful method to structure biomimetic films, given the stereospecific action of many enzymes.
Moraille
Patricia
P
Department of Chemistry and Centre for Self-Assembled Chemical Structures, Université de Montréal, C.P. 6128, succursale Centre-ville, Montréal, Quebec H3C 3J7, Canada.
Badia
Antonella
A
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D006868:Hydrolysis; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000737:chemistry; D010741:Phospholipases A / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013237:Stereoisomerism
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; C015153:1,2-dilauroylphosphatidylcholine; D015060:1,2-Dipalmitoylphosphatidylcholine; D010741:Phospholipases A