Genotyping of human neutrophil antigen-5a (Ond).
Antibodies to human neutrophil alloantigens (HNA) can cause immune-mediated neutropenias and transfusion complications. The diagnosis of antibodies to the HNA-5a (Ond) isoform of the alphaLbeta2 integrin is hampered by the lack of reliable methods for HNA-5a antigen typing. We have devised a polymerase chain reaction sequence-specific primer method (PCR-SSP) and used it to determine the HNA-5a gene frequencies in 320 individuals from different ethnicities. 15.3% were found to be HNA-5a negative, with no significant deviation between the populations. Results of HNA-5a genotyping were in accordance with phenotyping. Availability of HNA-5a PCR-SSP will facilitate the diagnosis of Ond antibody-mediated clinical conditions.
Sachs
U J H
UJ
Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany. ulrich.sachs@immunologie.med.uni-giessen.de
Reil
A
A
Bauer
C
C
Bux
J
J
Bein
G
G
Santoso
S
S
D000483:Alleles; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D044469:Racial Groups
D007519:Isoantigens; C451328:ond(a) antigen