Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine.
OBJECTIVE
To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action.


METHODS
MTT assay, immunohistochemical method and Western blotting were adopted.


RESULTS
IL (0.03 - 3 micromol x L(-1)) could inhibit the CASMCs proliferation induced by Phen (0.1 micromol x L(-1)) in a concentration-dependent manner. IL (0.1 micromol x L(-1)) antagonized Phen-induced overexpression of PDGF-beta and bFGF from 0.545 +/- 0.026 and 0.47 +/- 0.03 to 0.458 +/- 0.019 and 0.376 +/- 0.017 (P < 0.01 , P < 0.01). IL (0.1 micromol x L(-1)) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57 +/- 0.04, 0.44 +/- 0.04 and (173 +/- 36)% to 0.46 +/- 0.05, 0.372 +/- 0.021 and (115 +/- 35)% respectively (P < 0.01, P < 0.01, P < 0.01).


CONCLUSIONS
IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-beta, bFGF), protooncogene (c-fos, c-myc) and hsp70.
Xiao
Jun-hua
JH
Department of Pharmacology, Tongji Medical College, Wuhan 430030, China. xiaojunhuacao@yahoo.com.cn
Zhang
Yan-lin
YL
Ding
Li-li
LL
Feng
Xiu-ling
XL
Wang
Jia-ling
JL
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007546:Isoquinolines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D031653:Nelumbo / Q000737:chemistry; D010656:Phenylephrine / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism; D013552:Swine
D018840:HSP70 Heat-Shock Proteins; D007546:Isoquinolines; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D020574:Proto-Oncogene Proteins c-sis; C499779:isoliensinine; D016222:Fibroblast Growth Factor 2; D010656:Phenylephrine