title: Effect of testosterone and steroids homologues on indolamines and lipid peroxidation in rat brain.
abstract: The purpose of the present study was to evaluate the effect of 4-pregnen-17-hydroxy-3-one (A) and two steroids homologues: 3beta-acetoxy-5,16-pregnadien-20-one (B) and 3beta-acetoxy-16alpha-17alpha-epoxy-4-pregnen-20-one (C). Male Wistar rats were treated with o-cresol combined (A, B or C) steroids. Lipid peroxidation status as result of measurement reactive substances to thiobarbituric acid (TBARS) as well as serotonin (5-HT) and its precursor 5-hydroxytryptophan (5-HTP) were measured. The prostate glands were weighed, the 5alpha-reductase activity was determined. The animals treated with A, B, and C steroids showed a slight increase in both 5alpha-reductase activity and prostate size. 5-HT and 5-HTP levels did not change significantly, and TBARS showed an increase in the group treated with B steroid and a decrease in the A steroid group with significant differences in both groups (p<0.05) versus control group. Results suggest that A steroid reduces TBARS in rat brain, perhaps as a result of the interaction between the testosterone unsaturated carbons and OH(-) groups with free radicals.
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Avenida Imán No. 1, 3er piso, Colonia Cuicuilco, CP 04530 México City, Mexico.
authors.forename: David Calderón
authors.initials: DC
authors.lastname: Guzmán
authors.forename: Gerardo Barragán
authors.initials: GB
authors.lastname: Mejía
authors.forename: Ivonne Espitia
authors.initials: IE
authors.lastname: Vázquez
authors.forename: Ernestina Hernández
authors.initials: EH
authors.lastname: García
authors.forename: Daniel Santamaría
authors.initials: DS
authors.lastname: del Angel
authors.forename: Hugo Juárez
authors.initials: HJ
authors.lastname: Olguín
mesh_terms: D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D042944:Cholestenone 5 alpha-Reductase / Q000378:metabolism; D003408:Cresols / Q000494:pharmacology; D006908:Hydroxyprogesterones / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D011282:Pregnenediones / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: C469666:3-acetoxypregna-5,16-diene-20-one; D003408:Cresols; D006908:Hydroxyprogesterones; D011282:Pregnenediones; D017392:Thiobarbituric Acid Reactive Substances; C067746:17-hydroxypregn-4-ene-3-one; D012701:Serotonin; D013739:Testosterone; D006916:5-Hydroxytryptophan; D042944:Cholestenone 5 alpha-Reductase; C034047:2-cresol