Cyclopentane-modified PNA improves the sensitivity of nanoparticle-based scanometric DNA detection.
trans-Cyclopentane-modified PNA has been successfully utilized as a target capture strand to improve the detection limit of a known DNA detection assay, and provide high levels of mismatch discrimination.
Pokorski
Jonathan K
JK
Northwestern University and NIH, NIDDK, Laboratory of Bioorganic Chemistry, Bethesda, MD, USA.
Nam
Jwa-Min
JM
Vega
Rafael A
RA
Mirkin
Chad A
CA
Appella
Daniel H
DH
D000942:Antigens, Bacterial / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D020137:Base Pair Mismatch; D003517:Cyclopentanes / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D003517:Cyclopentanes; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; C030325:anthrax toxin; D004247:DNA