LC-MS determination of MPTP at sub-ppm level in pethidine hydrochloride.
An HPLC-MS with electrospray ionisation method for the determination of MPTP at sub-ppm level in pethidine hydrochloride has been developed and validated. Ionisation is performed by positive-ion electrospray and the quadrupole filter mass spectrometer is operated in the single ion recording mode. Chromatographic separation was achieved in gradient elution using a symmetry C18, 5 microm, 150 mm x 2.1 mm i.d. The mobile phase comprised water containing 0.1% formic acid (v/v) and acetonitrile containing 0.1% formic acid (v/v). The method showed to be linear in the range between 0.2 and 2.2 ng/ml, the estimated LOD was lower than 0.1 ng/ml and the LOQ was lower than 0.2 ng/ml.
Farina
Anna
A
Istituto Superiore di Sanità, viale Regina Elena n. 299, 00161 Rome, Italy. chimorg@iss.it
Gostoli
Gianluca
G
Bossù
Elena
E
Montinaro
Annalisa
A
Lestingi
Carmela
C
Lecce
Raffaele
R
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008614:Meperidine / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D008614:Meperidine; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine