Gravity-regulated proteins of Arabidopsis and cucumber seedlings detected by 2-D electrophoresis.
Gravity exerts a profound influence on plant growth. Proteomics may be the most promising technique to identify the proteins that are induced, repressed, or post-transcriptionally modified during gravity response in plants. In this research, the model plant, Arabidopsis grown in stationary and clino-rotated condition for 7 to 10 days and cucumber plant with the unique gravimorphogenesis were used. Namely, cucurbit seedlings develop a peg on the transition zone between the hypocotyls and root. Cucumber seedlings grown in a horizontal position on the ground suppress the development of the peg (negatively-controlled) on the upper side of the transition zone in response to gravity. Then, we analyzed gravity-regulated proteins expression by two-dimensional (2-D) gel electrophoresis.
Kamada
Motoshi
M
ISS Science Project Office, ISAS, JAXA.
Takaoki
Muneo
M
Ishioka
Noriaki
N
D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000032:analysis; D018553:Cucumis sativus / Q000254:growth & development*; D006112:Gravitation; D018466:Gravity Sensing / Q000502:physiology*; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics; D012399:Rotation; D036226:Seedlings / Q000254:growth & development*
D029681:Arabidopsis Proteins; D010940:Plant Proteins