The distinct effects of a butanol fraction of Bidens pilosa plant extract on the development of Th1-mediated diabetes and Th2-mediated airway inflammation in mice.
Bidens pilosa is claimed to be useful for immune or anti-inflammatory disorders; however, little scientific evidence has been published concerning its function. In this paper, immune disease mouse models were used to study the function of a butanol fraction of B.pilosa. We demonstrated treatment with the butanol fraction of B.pilosa ameliorated Th1 cell-mediated autoimmune diabetes in nonobese diabetic (NOD) mice but caused deterioration of Th2 cell-mediated airway inflammation induced by ovalbumin (OVA) in BALB/c mice. We next showed that Th2 cytokines (IL-4 and/or IL-5) increased but Th1 cytokine (IFN-gamma) decreased following injections with the butanol fraction of B.pilosa in both mouse strains. Accordingly, Th2 cytokine-regulated IgE production in mouse serum increased following treatment with this fraction. Finally, we found that the butanol fraction of B.pilosa inhibited Th1 cell differentiation but promoted Th2 cell differentiation. Taken together, the butanol fraction of B.pilosa has a dichotomous effect on helper T cell-mediated immune disorders, plausibly via modulation of T cell differentiation.
Chang
Cicero Lee-Tian
CL
Institute of BioAgricultural Sciences, Academia Sinica, Taiwan, ROC.
Kuo
Hui-Kai
HK
Chang
Shu-Lin
SL
Chiang
Yi-Ming
YM
Lee
Tsung-Han
TH
Wu
Wen-Mein
WM
Shyur
Lie-Fen
LF
Yang
Wen-Chin
WC
D000818:Animals; D031176:Bidens; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D000440:Butanols / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D010047:Ovalbumin / Q000633:toxicity; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology
D000440:Butanols; D016207:Cytokines; D004365:Drugs, Chinese Herbal; C442780:plant extract, Bidens pilosa; D007073:Immunoglobulin E; D010047:Ovalbumin