Reference ranges of lymphocyte subsets of healthy adults in Turkey.
The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey.


METHODS
Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 +/- 16.69).


RESULTS
The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 +/- 8.44%, 1,680 +/- 528 cells/microl; CD4: 47.37 +/- 9.10%, 1,095 +/- 391 cells/microl; CD8: 28.99 +/- 5.99%, 669 +/- 239 cells/microl; CD19: 10.96 +/- 4.44%, 254 +/- 122 cells/microl and CD56: 7.03 +/- 3.26%, 161 +/- 92 cells/microl, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05).


CONCLUSIONS
Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey.
Yaman
Akgun
A
Department of Microbiology, Faculty of Medicine, Cukurova University, Balcali-Adana, Turkey. kyaman@cu.edu.tr
Cetiner
Salih
S
Kibar
Filiz
F
Taşova
Yeşim
Y
Seydaoğlu
Gulşah
G
Dündar
Ismail H
IH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D016516:CD4-CD8 Ratio; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014421:Turkey