title: [Evaluation of different liquid media for the culture of Gardnerella vaginalis and characterization of the protein profile with PAGE-SDS electrophoresis].
abstract: 3 liquid culture media: triptose soya broth supplemented with equine serum (TSB-es)/without supplement (TSB) and Casman broth (CB) were evaluated under different incubation conditions at 10% of CO2 and under aerobic conditions. There were no significant differences in the values of optic density for the TSB-es and CB media. However, on establishing the protein profile by PAGE-SDS for the evaluated conditions, the presence of protein of 14 kDa and the values of optic density allowed the selection of the CB medium for the culture of Gardnerella vaginalis. The sensitivity to different pH in this medium was studied. pH 7.2 proved to be optimum and it was established its growth cynetics. The analysis by PAGE-SDS of the complete cells corresponding to different clinical isolates of G. vaginalis with biochemical variations made possible to characterize the protein profile in this medium and showed similarity between the isolates.
authors.affiliation: Centro Nacionial de Sanidad Agropecuaria, Autopista Nacional San José de las Lajas. espinosa@censa.edu.cu
authors.forename: Ivette
authors.initials: I
authors.lastname: Espinosa
authors.forename: Maité
authors.initials: M
authors.lastname: Lorenzo
authors.forename: Yamilka
authors.initials: Y
authors.lastname: Riverón
authors.forename: Elba
authors.initials: E
authors.lastname: Alvarez
authors.forename: Alejandra
authors.initials: A
authors.lastname: Villoch
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006194:Gardnerella vaginalis / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media