Scallop DMT functions as a Ca2+ transporter.
We identified a DMT (divalent metal transporter) homologous protein that functions as a Ca(2+) transporter. Scallop DMT cDNA encodes a 539-amino-acid protein with 12 putative membrane-spanning domains and has a consensus transport motif in the fourth extracellular loop. Since its mRNA is significantly expressed in the gill and intestine, it is assumed that scallop DMT transports Ca(2+) from seawater by the gill and from food by the intestine. Scallop DMT lacks the iron-responsive element commonly found in iron-regulatory proteins, suggesting that it is free of the post-transcriptional regulation from intracellular Fe(2+) concentration. Scallop DMT distinctly functions as a Ca(2+) transporter unlike other DMTs, however, it also transports Fe(2+) and Cd(2+) similar to them.
Toyohara
Haruhiko
H
Laboratory of Marine Biological Function, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan. toyohara@kais.kyoro-u.ac.jp
Yamamoto
Sayuri
S
Hosoi
Masatomi
M
Takagi
Masaya
M
Hayashi
Isao
I
Nakao
Kenji
K
Kaneko
Shuji
S
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D007501:Iron / Q000378:metabolism; D008845:Microinjections; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014981:Xenopus
D027682:Cation Transport Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D002104:Cadmium; D007501:Iron; D002118:Calcium