Biodegradation of beta-cyfluthrin by Pseudomonas stutzeri strain S1.
beta-Cyfluthrin [alpha-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate] pesticide has been in agricultural use in the recent years for controlling Lepidopteran pests affecting solanaceous crops. The extensive use of synthetic pyrethroids like beta-cyfluthrin has resulted in wide spread environmental contamination. The purpose of this study was to isolate bacteria from soil and to determine their ability to degrade beta-cyfluthrin and identify the intermediates in culture broth using spectroscopy. An aerobic bacterium capable of degrading beta-cyfluthrin was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain S1) had 100% identity to the sequence from Pseudomonas stutzeri. Finally products formed during degradation of beta-cyfluthrin have been identified as alpha-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate (M.W. 341); 4-fluoro-3-phenoxy-alpha-cyanobenzyl alcohol (M.W. 243) and 3(2,2-dichlorovinyl)-2,2-dimethyl cyclopropanecarboxylic acid (M.W. 208).
Saikia
Nirmali
N
Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110 012, India.
Das
Subrata K
SK
Patel
Bharat K C
BK
Niwas
Ram
R
Singh
Aqbal
A
Gopal
Madhuban
M
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008954:Models, Biological; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011722:Pyrethrins / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012989:Soil Pollutants; C052570:cyfluthrin