[Amplification of EMS1 gene in oral carcinogenesis].
OBJECTIVE
To investigate the amplification of EMS1 gene in the carcinogenesis of oral mucosa.


METHODS
A total of 78 subjects, including 30 patients with oral leukoplakia (OLK), 33 with oral squamous cell carcinoma (OSCC), and 15 healthy controls, were studied. By using microdissection method, we obtained normal mucosa, hyperplastic epithelia, mild-dysplastic epithelia, moderate-dysplastic epithelia, severe-dysplastic epithelia and primary OSCC tissue. Then we analyzed EMS1 amplification by using differential PCR.


RESULTS
EMS1 amplification began from moderate-dysplastic epithelia and occurred in 20.0% OLK cases and 57.6% OSCC cases. In the progress of OSCC, no gene amplification was observed in normal tissues, non-dysplastic OLK and mild-dysplastic OLK, while in the cases with metastasis, amplification frequency increased significantly (P = 0.015).


CONCLUSIONS
EMS1 amplification parallels with the progress of oral carcinogenesis, indicating their potential roles in oral carcinogenesis.
Xia
Juan
J
Department of Oral Medicine, Guanghua School of Stomatology, Sun-Yat Sen University, Guangzhou 510055, China.
Li
Bing-qi
BQ
Zeng
Xin
X
Chen
Qian-ming
QM
He
Yuan
Y
Cai
Yang
Y
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D051356:Cortactin / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics* / Q000473:pathology
C495868:CTTN protein, human; D051356:Cortactin