[The development of yeast vector system for study of regulational functions of eukaryotic noncoding elements].
The original yeast vector system was proposed for study of regulation functions of eukaryotic noncoding sequences. This system consists of two reporter genes that arranges in opposition to one another. The promoter activity of LTR HERV-K of locus 22-19 in 7p22 human chromosome was studied. It was shown that the LTR initiates transcription of reporter gene in yeasts in forward and reverse orientation with respect to the reporter. It was displayed that the LTR 22-19 HERV-K possesses bidirectional promoter activity in the yeast vector system. Comparison of the promoter activity LTR 22-19 and strong GAL1 and TDH promoters in yeasts Saccharomyces cerevisiae shown that promoter activity of the LTR amount approximately 0.34% for promoter activity of the inducible GAL1 promoter and 0.26%--of the constitutive TDH promoter.
Abdeeva
I A
IA
Komakhin
R A
RA
Musiĭchuk
K A
KA
Goldenkova
I V
IV
D001483:Base Sequence; D002897:Chromosomes, Human, Pair 7; D004247:DNA; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020079:Terminal Repeat Sequences; D014158:Transcription, Genetic / Q000235:genetics
D004247:DNA