title: [Effects of tea polyphenols and tea pigments on apoptosis in HepG2 cell line].
abstract: OBJECTIVE
  To investigate the effect of tea polyphenols and tea pigments on apoptosis in HepG2 cells.
  
  
  METHODS
  HepG2 cells were seeded at a density of 5 x 10(5)/well in six-well culture dishes. The cells were then treated with 50 or 100 mg/L tea pigments and harvested at 48h by trypsinization. Agrose electrophoresis was applied to investigate DNA-LADDER, Bcl-2 and Bax protein expression were detected by Western blot.
  
  
  RESULTS
  Tea polyphenols and tea pigments induced the appearance of DNA-LADDER; Western blot analysis demonstrated that Bcl-2 expression was significantly inhibited and the expression of Bax was significantly induced by tea polyphenols and tea pigments.
  
  
  CONCLUSIONS
  Induction of apoptosis may be an important mechanism of cancer chemoprevention by tea.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Jia
authors.forename: Chi
authors.initials: C
authors.lastname: Han
authors.forename: Jun-Shi
authors.initials: JS
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010636:Phenols / Q000494:pharmacology*; D010860:Pigments, Biological / Q000494:pharmacology*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010636:Phenols; D010860:Pigments, Biological; D059808:Polyphenols; D013662:Tea