title: Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland.
abstract: We examined the expression and immunolocalization of water-channel aquaporins in the mammary gland by reverse transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry. RT-PCR and immunoblotting revealed the expression of aquaporin-1 (AQP1) and AQP3 in the lactating rat mammary gland. AQP3 was detected in the alveolar epithelium and duct system whereas AQP1 was found in the capillaries and venules. AQP3 was present in the basolateral membrane of secretory epithelial cells and intralobular and interlobular duct epithelial cells. The main duct near the orifice in the nipple, which is comprised of a stratified epithelium, bore AQP3 in its basal and intermediate layers. AQP1 was located in both the apical and basolateral membranes of capillary and venule endothelia. AQP3 was not detected in virgin females. AQP3 was found in some differentiating mammary epithelial cells in the pregnant rat. AQP1 was present in capillaries and venules in the differentiating mammary gland of the pregnant rat and in the mammary fat pad of virgin females. We found a similar distribution of AQP1 and AQP3 in the mouse. AQP1 and AQP3 seem to play roles in the synthesis and/or secretion of milk.
authors.affiliation: Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Showa-machi 3-39-22, Maebashi, Gunma 371-8511, Japan. matoshi@med.gunma-u.ac.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: Natsuko
authors.initials: N
authors.lastname: Machida
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tajika
authors.forename: Abdushukur
authors.initials: A
authors.lastname: Ablimit
authors.forename: Takeshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Takeo
authors.initials: T
authors.lastname: Aoki
authors.forename: Haruo
authors.initials: H
authors.lastname: Hagiwara
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Takata
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D051398:Aquaporin 1; D051399:Aquaporin 2; D051400:Aquaporin 3; D020346:Aquaporins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C496021:Aqp1 protein, mouse; C496022:Aqp1 protein, rat; C496024:Aqp2 protein, mouse; C496025:Aqp2 protein, rat; C496028:Aqp3 protein, mouse; C496029:Aqp3 protein, rat; D051399:Aquaporin 2; D020346:Aquaporins; D051398:Aquaporin 1; D051400:Aquaporin 3