title: Cloning and characterization of the 5'-flanking region of the rat estrogen receptor beta gene.
abstract: In the rat, estrogen receptor (ER) beta is preferentially expressed in the ovary and the prostate gland where it is transcriptionally regulated by testosterone. A single 5'-end of rERbeta cDNA was identified in these tissues by the 5'-RACE analysis in the present study. The transcription starting site was predicted at -335 from the translation starting signal (ATG), and a 640bp section of the 5'-flanking region of the gene was cloned. Luciferase reporter assays revealed this region to be responsible for cell-specific promoter activity and successive deletion analyses indicated that only 98bp were sufficient for basic promoter activity as well as for testosterone-dependent transcription. The sequence of the determined region found to demonstrate high homology with the mouse ERbeta promoter with more than 80% identity between positions -1 and -550. The rat region of -30/-110 also showed good homology with 69% identity to corresponding section of the human promoter. Putative cis-acting elements, USF/Arnt and AML1a, were found in common in the promoter regions of three species. The present study thus demonstrated the 5'-flanking region of the rERbeta gene to be a functional promoter.
authors.affiliation: Department of Developmental Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan. nfjm@hiroshima-u.ac.jp
authors.forename: Nariaki
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Kosuke
authors.initials: K
authors.lastname: Asano
authors.forename: Tsuguru
authors.initials: T
authors.lastname: Usui
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Honda
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000502:physiology; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000502:physiology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D018076:DNA, Complementary; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D008156:Luciferases