Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges.
Both amyloid-prone cystatin and unstable mutant C94A lysozyme were secreted in wild-type and Deltaeps1 Saccharomyces cerevisiae cells. Amyloid-prone cystatin secreted at much higher level in Deltaeps1 cells than that in wild-type yeast. In parallel, the secretion amount of disulfide bond disrupted mutant C94A lysozyme greatly increased in Deltaeps1 cells although that was apparently low in wild-type yeast cells compared with the secretion amount of wild-type lysozyme. It is interesting that neither the unstable mutant C94A lysozyme nor amyloid-prone cystatin secreted in Deltaeps1 cells maintained their specific activities. These observations lead to the supposition that yeast cells deficient for the protein disulfide isomerase-family-member EPS1 locus secrete more of labile disulfide-containing model proteins.
He
Jianwei
J
Department of Biological Chemistry, Yamaguchi University, Japan.
Sakamoto
Takashi
T
Song
Youtao
Y
Saito
Akira
A
Harada
Akihito
A
Azakami
Hiroyuki
H
Kato
Akio
A
D000682:Amyloid / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002645:Chickens; D015891:Cystatins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D000682:Amyloid; D015891:Cystatins; D004220:Disulfides; C402288:Eps1 protein, S cerevisiae; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D009113:Muramidase; D003545:Cysteine