title: AIP1, a water-soluble fraction from Artemisia iwayomogi, suppresses thymocyte apoptosis in vitro and down-regulates the expression of Fas gene.
abstract: The AIP1 fraction, a small water-soluble fraction purified from Artemisia iwayomogi, was shown to increase antibody production and suppress transplanted tumor cell growth in mice. In order to understand its immuno-modulating activity, we have examined the effect of the AIP1 on mouse thymocytes in vitro. Treatment of mouse thymocytes in culture with the fraction resulted in the suppression of the cell death and the extension of the cell survival. A mouse gene array provided a profile of gene expression change showing the pattern of up- and down-regulated genes by the AIP1 treatment, suggesting that the Fas/FasL-dependent apoptosis pathway might be modulated by the fraction.
authors.affiliation: School of Biotechnology & Biomedical Science and Bio-Health Product Research Center, Inje University, Gimhae 621-749, Korea.
authors.forename: Jung Suk
authors.initials: JS
authors.lastname: Hwang
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Ji
authors.forename: Kyung Ah
authors.initials: KA
authors.lastname: Koo
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Lee
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Yeo
authors.forename: Seon Woo
authors.initials: SW
authors.lastname: Cheong
authors.forename: Joo Hung
authors.initials: JH
authors.lastname: Park
authors.forename: Gap Soo
authors.initials: GS
authors.lastname: Oh
authors.forename: Chun Sik
authors.initials: CS
authors.lastname: Yoon
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Youn
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D019306:Artemisia; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005591:Chemical Fractionation; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007155:Immunologic Factors / Q000302:isolation & purification / Q000502:physiology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012995:Solubility; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors / Q000096:biosynthesis*; D014867:Water
chemical_list: D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007155:Immunologic Factors; D008562:Membrane Glycoproteins; D010936:Plant Extracts; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors; D014867:Water