Methods for reducing bacterial contamination in the BacT/Alert mycobacterial culture detection system.
Two methods were compared for decreasing bacterial contamination in the BacT/Alert mycobacterial culture detection system. Two concentrations, 0.5 ml (standard amount) and 1.0 ml, of mycobacterial antibiotic supplement were evaluated. Contamination rates were 14% and 6% for the standard and the doubled concentrations, respectively. This difference was statistically significant (P < 0.005).
Dionne
Kim
K
Division of Medical Microbiology, Johns Hopkins University, Meyer B1-193, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Sweeney
Amy
A
Hedgepeth
Annie
A
Carroll
Karen
K
Parrish
Nicole
N
D004866:Equipment Contamination; D008826:Microbial Sensitivity Tests / Q000379:methods; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification*; D014640:Vancomycin / Q000494:pharmacology
D014640:Vancomycin