The role of thrombofilia related to Factor V Leiden and Factor II G20210A mutations in recurrent abortions.
OBJECTIVE
To study, the impact of thrombophilia caused by Factor V, Factor ll G20210A mutations on recurrent abortions, the prevelance of Factor V Leiden and Factor II G20210A mutation in patients with habitual abortions.


METHODS
Forty one patients with a diagnosis of habitual abortion were enrolled in the study. Control group consisted of 50 women without a history of poor obstetric outcome. 10 ml. peripheral venous blood was taken from study and control groups and transferred to EDTA tubes, and were tested for Factor V Leiden and Factor II G20210A mutations by PCR in genetics laboratory.


RESULTS
Phenotypes of 91 cases were determined by PCR for Factor V Leiden. It was established that of 41 patients in the study group, 31 (75.6%) had GG genotype, 9 (22%) had GA genotype and 1 (2.4%) had AA genotype. In the control group, 45 (90%) of 50 women had GG genotype and 5 (10%) had GA genotype. A allele carrier status was found to be 24.4% in study group and 10% in the control group. The difference between them was not statistically significant (p = 0.06).


CONCLUSIONS
The results obtained from patients and control group have no difference in Factor V Leiden and Factor II G20210A mutations.These results suggest that mutations have no role in etiology of 1. and 2. trimester recurrent abortions.
Aksoy
Muge
M
Department of Obstetrics and Gynecology, Ankara University School of Medicine Cebeci, Ankara, Turkey.
Tek
Ibrahim
I
Karabulut
Halil
H
Berker
Bulent
B
Soylemez
Feride
F
D000026:Abortion, Habitual / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005165:Factor V / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D010154:Pakistan / Q000453:epidemiology; D010641:Phenotype; D021041:Placental Circulation / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D018566:Pregnancy, High-Risk / Q000235:genetics; D015995:Prevalence; D011516:Prothrombin / Q000235:genetics*; D012307:Risk Factors; D019851:Thrombophilia / Q000150:complications* / Q000453:epidemiology / Q000235:genetics
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin