Antimutagenic constituents from the thorns of Gleditsia sinensis.
Antimutagenic activity-guided fractionation of an extract prepared from the thorns of Gleditsia sinensis LAM. led to the isolation of one triterpenoid and four steroids, which were identified as D:C-friedours-7-en-3-one (1), stigmast-4-ene-3,6-dione (2), stigmastane-3,6-dione (3), stigmasterol (4), and beta-sitosterol (5). Triterpenoid 1 was found for the first time in a natural source and the steroids 2-5 were first isolated from this plant. Stigmasterol was the most active antimutagen, showing 51.2% and 64.2% reduction of the induction factor against the mutagens MNNG and NQO, respectively, in the SOS chromotest. Some NMR data of the steroids 2 and 3 obtained have to be revised.
Lim
Jae-Chul
JC
Department of Biotechnology, Dongguk University, Gyeongju 780-714, Korea.
Park
Jong Hee
JH
Budesinsky
Milos
M
Kasal
Alexander
A
Han
Yeong-Hwan
YH
Koo
Byung-Soo
BS
Lee
Seung-Il
SI
Lee
Dong-Ung
DU
D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031501:Gleditsia; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018514:Plant Structures; D013234:Stem Cells / Q000187:drug effects
D016587:Antimutagenic Agents; D010936:Plant Extracts