title: Candidate gene database and transcript map for peach, a model species for fruit trees.
abstract: Peach (Prunus persica) is a model species for the Rosaceae, which includes a number of economically important fruit tree species. To develop an extensive Prunus expressed sequence tag (EST) database for identifying and cloning the genes important to fruit and tree development, we generated 9,984 high-quality ESTs from a peach cDNA library of developing fruit mesocarp. After assembly and annotation, a putative peach unigene set consisting of 3,842 ESTs was defined. Gene ontology (GO) classification was assigned based on the annotation of the single "best hit" match against the Swiss-Prot database. No significant homology could be found in the GenBank nr databases for 24.3% of the sequences. Using core markers from the general Prunus genetic map, we anchored bacterial artificial chromosome (BAC) clones on the genetic map, thereby providing a framework for the construction of a physical and transcript map. A transcript map was developed by hybridizing 1,236 ESTs from the putative peach unigene set and an additional 68 peach cDNA clones against the peach BAC library. Hybridizing ESTs to genetically anchored BACs immediately localized 11.2% of the ESTs on the genetic map. ESTs showed a clustering of expressed genes in defined regions of the linkage groups. [The data were built into a regularly updated Genome Database for Rosaceae (GDR), available at (http://www.genome.clemson.edu/gdr/).].
authors.affiliation: Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, SC 29634, USA.
authors.forename: Renate
authors.initials: R
authors.lastname: Horn
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Lecouls
authors.forename: Ann
authors.initials: A
authors.lastname: Callahan
authors.forename: Abhaya
authors.initials: A
authors.lastname: Dandekar
authors.forename: Lilibeth
authors.initials: L
authors.lastname: Garay
authors.forename: Per
authors.initials: P
authors.lastname: McCord
authors.forename: Werner
authors.initials: W
authors.lastname: Howad
authors.forename: Helen
authors.initials: H
authors.lastname: Chan
authors.forename: Ignazio
authors.initials: I
authors.lastname: Verde
authors.forename: Doreen
authors.initials: D
authors.lastname: Main
authors.forename: Sook
authors.initials: S
authors.lastname: Jung
authors.forename: Laura
authors.initials: L
authors.lastname: Georgi
authors.forename: Sam
authors.initials: S
authors.lastname: Forrest
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mook
authors.forename: Tatyana
authors.initials: T
authors.lastname: Zhebentyayeva
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Christopher
authors.initials: C
authors.lastname: Jesudurai
authors.forename: Bryon
authors.initials: B
authors.lastname: Sosinski
authors.forename: Pere
authors.initials: P
authors.lastname: Arús
authors.forename: Vance
authors.initials: V
authors.lastname: Baird
authors.forename: Dan
authors.initials: D
authors.lastname: Parfitt
authors.forename: Gregory
authors.initials: G
authors.lastname: Reighard
authors.forename: Ralph
authors.initials: R
authors.lastname: Scorza
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Tomkins
authors.forename: Rod
authors.initials: R
authors.lastname: Wing
authors.forename: Albert Glenn
authors.initials: AG
authors.lastname: Abbott
mesh_terms: D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D015723:Gene Library; D018745:Genome, Plant; D010957:Plasmids / Q000235:genetics; D027861:Prunus / Q000235:genetics*; D017422:Sequence Analysis, DNA