title: Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions.
abstract: An immunohistochemistry (IHC) procedure for the detection of Campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of Campylobacter spp. abortion (four ovine and 22 bovine). The species of Campylobacter isolated included C. fetus ssp. venerealis from 13 bovine fetuses, C. fetus ssp. fetus from two ovine and one bovine fetus, Campylobacter jejuni from seven bovine fetuses, Campylobacter lari from two ovine fetuses and an unspeciated Campylobacter species in one bovine fetus. Histologic lesions identified in the aborted fetuses included placentitis, serositis, pneumonia, gastroenteritis, hepatitis and encephalitis. Campylobacter fetus antigens were identified by IHC in 13 of 13 bovine fetuses from which C. fetus ssp. venerealis was isolated and in two of two ovine fetuses from which C. fetus ssp. fetus was isolated. The IHC stains were negative in tissues from seven bovine fetuses from which C. jejuni was isolated, one bovine fetus infected with C. fetus ssp. fetus, one bovine fetus infected with the unspeciated Campylobacter and two ovine fetuses infected with C. lari. In positive cases, the IHC stain most frequently identified bacteria in the lung and gastrointestinal tract. The C. fetus IHC procedure performed on formalin fixed tissues is a practical tool for the diagnosis of natural cases of ovine and bovine abortion caused by C. fetus.
authors.affiliation: Animal Health Group, Instituto Nacional de Tecnología Agropecuaria (INTA), CC 276 (7620) Balcarce, Argentina. ccampero@balcarce.inta.gov.ar
authors.forename: C M
authors.initials: CM
authors.lastname: Campero
authors.forename: M L
authors.initials: ML
authors.lastname: Anderson
authors.forename: R L
authors.initials: RL
authors.lastname: Walker
authors.forename: P C
authors.initials: PC
authors.lastname: Blanchard
authors.forename: L
authors.initials: L
authors.lastname: Barbano
authors.forename: P
authors.initials: P
authors.lastname: Chiu
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: G
authors.initials: G
authors.lastname: Combessies
authors.forename: J C
authors.initials: JC
authors.lastname: Bardon
authors.forename: J
authors.initials: J
authors.lastname: Cordeviola
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary*; D011247:Pregnancy; D012189:Retrospective Studies; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology