Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis.
Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.
Kudla
Urszula
U
Laboratory of Nematology, Graduate School for Experimental Plant Sciences, Wageningen University, The Netherlands.
Qin
Ling
L
Milac
Adina
A
Kielak
Anna
A
Maissen
Cyril
C
Overmars
Hein
H
Popeijus
Herman
H
Roze
Erwin
E
Petrescu
Andrei
A
Smant
Geert
G
Bakker
Jaap
J
Helder
Johannes
J
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D016678:Genome; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000469:parasitology*; D014026:Nicotiana / Q000235:genetics; D017187:Tylenchida / Q000737:chemistry* / Q000235:genetics*
D018076:DNA, Complementary; D015801:Helminth Proteins