Significant species-dependence of P700 redox potential as verified by spectroelectrochemistry: comparison of spinach and Theromosynechococcus elongatus.
The redox potentials of P700, the primary electron donor of photosystem (PS) I, of spinach and Thermosynechococcus elongatus were determined by means of spectroelectrochemistry with an error range of +/-2-3 mV, to find that the redox potential of P700 in T. elongatus is lower by ca. 50 mV as compared with spinach. The shift in the P700 redox potential of PS I core particles prepared by harsh detergent treatments remained to within 10 mV for both organisms. These results show that the 50 mV difference in the P700 redox potential between the two organisms is not a detergent-induced artifact but reflects an intrinsic property of each PS I.
Nakamura
Akimasa
A
Institute of Industrial Science, The University of Tokyo, Japan. akimasa.nakamura@jr-central.co.jp
Suzawa
Tomoyuki
T
Kato
Yuki
Y
Watanabe
Tadashi
T
D000458:Cyanobacteria / Q000737:chemistry* / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D004563:Electrochemistry; D010084:Oxidation-Reduction / Q000187:drug effects; D045331:Photosystem I Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D015203:Reproducibility of Results; D013045:Species Specificity; D013057:Spectrum Analysis; D018724:Spinacia oleracea / Q000737:chemistry* / Q000378:metabolism*
D003902:Detergents; D045331:Photosystem I Protein Complex