Assignment of the gene for porcine insulin-like growth factor binding protein 1 to chromosome 18 and detection of polymorphisms in intron 2 by PCR-RFLP.
We have obtained a partial cDNA and three BAC clones for the porcine insulin-like growth factor binding protein 1 gene (IGFBP-1). Results of fluorescence in situ and radiation hybrid (RH) mapping assigned this gene to porcine chromosome (SSC) 18q24-qter. We found two types of polymerase chain reaction-restriction-fragment-length polymorphisms (PCR-RFLP) in intron 2 by using FokI and AluI.
Inage-Miyake
Yuko
Y
Society for Techno-innovation of Agriculture, Forestry and Fisheries Institute, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Ibaraki, Japan.
Shimanuki
Shin-ichi
S
Itoh
Tomohito
T
Murakami
Yasuto
Y
Kimura
Masahiro
M
Suzuki
Hideaki
H
Miyake
Masashi
M
Toki
Daisuke
D
Uenishi
Hirohide
H
Awata
Takashi
T
Hamasima
Noriyuki
N
D000818:Animals; D002874:Chromosome Mapping; D017404:In Situ Hybridization, Fluorescence; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics*; D007438:Introns / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*
D018970:Insulin-Like Growth Factor Binding Protein 1