title: Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates.
abstract: OBJECTIVE
  To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate.
  
  
  METHODS
  Prospective-controlled study.
  
  
  METHODS
  Andrology department at a university-based medical institution.
  
  
  METHODS
  Ten healthy volunteer sperm donors.
  
  
  METHODS
  Sperm populations were separated using annexin-V magnetic-activated cell sorting before and after the cryopreservation-thawing process.
  
  
  METHODS
  Sperm motility and cryosurvival rate.
  
  
  RESULTS
  Annexin-negative sperm separated by magnetic-activated cell sorting had statistically significantly higher motility following cryopreservation-thawing than sperm that were not separated. Similarly, annexin-negative spermatozoa also had higher cryosurvival rate than sperm cryopreserved without magnetic-activated cell sorting and sperm that were annexin-positive.
  
  
  CONCLUSIONS
  Superparamagnetic annexin V-conjugated microbeads can separate spermatozoa with externalized phosphatidylserine, which is considered one of the early features of late apoptosis. The separation of a distinctive population of nonapoptotic spermatozoa with intact membranes may optimize the cryopreservation-thawing outcome. Magnetic-activated cell sorting using annexin-V microbeads enhances sperm motility and cryosurvival rates following cryopreservation.
authors.affiliation: Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Cleveland, Ohio 44195, USA.
authors.forename: Tamer M
authors.initials: TM
authors.lastname: Said
authors.forename: Sonja
authors.initials: S
authors.lastname: Grunewald
authors.forename: Uwe
authors.initials: U
authors.lastname: Paasch
authors.forename: Manja
authors.initials: M
authors.lastname: Rasch
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.forename: Hans-Juergen
authors.initials: HJ
authors.lastname: Glander
mesh_terms: D002469:Cell Separation / Q000379:methods* / Q000706:statistics & numerical data; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods* / Q000706:statistics & numerical data; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008280:Magnetics; D008297:Male; D011446:Prospective Studies; D012662:Semen Preservation / Q000379:methods* / Q000706:statistics & numerical data; D013081:Sperm Motility / Q000502:physiology*