Impact of polychlorinated biphenyl Aroclor 1254 on testicular antioxidant system in adult rats.
To clarify the reproductive toxicity of polychlorinated biphenyl compounds through determination of testicular lipid peroxidation, reactive oxygen species and enzymatic and non-enzymatic antioxidants in rats exposed to Aroclor 1254. Adult male rats were administered Aroclor 1254 at a dose of 2 mg/kg per day ip for 30 days. The rats were sacrificed 24 hours after last dosing and the serum and other tissues collected and processed for relevant determinations. The body weight and the weights of the testis, epididymis, ventral prostate and seminal vesicle and the serum testosterone and estradiol were significantly decreased in Aroclor 1254 treated rats. The testicular lipid peroxidation, hydrogen peroxide and hydroxyl radical were significantly elevated whereas, testicular antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and glutathione reductase (GR) were significantly decreased. The non-enzymatic antioxidants, vitamin C and vitamin E, were also decreased. These results suggest that Aroclor 1254 induces an increase in the lipid peroxidation, hydrogen peroxide and hydroxyl radical and diminish in the antioxidant defense system in rats, indicating that the free radical-dependent mechanism may play an important role in the testicular toxicity of polychlorinated biphenyls.
Murugesan
P
P
Department of Endocrinology, Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
Senthilkumar
J
J
Balasubramanian
K
K
Aruldhas
M M
MM
Arunakaran
J
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D013956:Antithyroid Agents / Q000633:toxicity*; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
D000975:Antioxidants; D013956:Antithyroid Agents; D017382:Reactive Oxygen Species; D020111:Chlorodiphenyl (54% Chlorine)