Anxious and hyperactive phenotype following brief ischemic episodes in mice.
BACKGROUND
Poststroke emotional and behavioral abnormalities have an impact on outcome but have scarcely been characterized in animal models. We tested whether brief ischemic episodes induce behavioral changes in mice.


METHODS
129/Sv mice were subjected to 30-min occlusion of left or right middle cerebral artery (MCAo) followed by reperfusion or sham operation (n = 9 or 10 per group). Eight to ten weeks later, mice were tested for spontaneous locomotor activity, anxiety in the elevated plus maze, and depressive behavior in the modified Porsolt forced swim test. Outcome was correlated to monoamine and amino acid levels and compared with histologic damage at 10 weeks.


RESULTS
Ischemia was associated with increased activity (right MCAo) and anxiety (left MCAo), but not poststroke depression. Noradrenaline increased by 30%-45% in the ischemic striatum and correlated with locomotor activity (r = .48); dopamine and homovanillinic acid were decreased compared with sham. The lesion was confined to the striatum, and scattered neuronal death was observed in a number of remote brain regions.


CONCLUSIONS
Brief ischemic episodes in the mouse induce an anxious, hyperactive but not depressive phenotype that may relate to left versus right hemispheric lesion location, alterations in brain monoamine levels, and selective neurodegeneration.
Winter
Benjamin
B
Klinik und Poliklinik für Neurologie der Charité, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Juckel
Georg
G
Viktorov
Ilya
I
Katchanov
Juri
J
Gietz
Andrea
A
Sohr
Reinhard
R
Balkaya
Mustafa
M
Hörtnagl
Heide
H
Endres
Matthias
M
D000818:Animals; D001007:Anxiety / Q000209:etiology / Q000378:metabolism / Q000523:psychology*; D015306:Biogenic Monoamines / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D003863:Depression / Q000523:psychology; D006948:Hyperkinesis / Q000209:etiology / Q000378:metabolism / Q000523:psychology*; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000523:psychology; D002546:Ischemic Attack, Transient / Q000150:complications / Q000378:metabolism / Q000523:psychology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism; D010641:Phenotype; D015427:Reperfusion Injury / Q000523:psychology; D013550:Swimming / Q000523:psychology
D015306:Biogenic Monoamines; D018377:Neurotransmitter Agents