Comparative analysis of differentially expressed genes in Shewanella oneidensis MR-1 following exposure to UVC, UVB, and UVA radiation.
We previously reported that Shewanella oneidensis MR-1 is highly sensitive to UVC (254 nm), UVB (290 to 320 nm), and UVA (320 to 400 nm). Here we delineated the cellular response of MR-1 to UV radiation damage by analyzing the transcriptional profile during a 1-h recovering period after UVC, UVB, and UVA exposure at a dose that yields about a 20% survival rate. Although the SOS response was observed with all three treatments, the induction was more robust in response to short-wavelength UV radiation (UVB and UVC). Similarly, more prophage-related genes were induced by short-wavelength UV radiation. MR-1 showed an active detoxification mechanism in response to UVA, which included the induction of antioxidant enzymes and iron-sequestering proteins to scavenge reactive oxygen species. In addition, a great number of genes encoding multidrug and heavy metal efflux pumps were induced following UVA irradiation. Our data suggested that activation of prophages appears the major lethal factor in MR-1 following UVC or UVB irradiation, whereas oxidative damage contributes greatly to the high UVA sensitivity in MR-1.
Qiu
Xiaoyun
X
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
Sundin
George W
GW
Wu
Liyou
L
Zhou
Jizhong
J
Tiedje
James M
JM
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics* / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D019216:Metals, Heavy / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D039002:Prophages / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D018435:ATP Binding Cassette Transporter, Subfamily B; D001427:Bacterial Toxins; D002352:Carrier Proteins; D019216:Metals, Heavy; D017382:Reactive Oxygen Species; D045643:DNA Repair Enzymes