[The total antioxidant capacity and low-molecular antioxidant concentration in plasma of type-2 diabetes patients with different stage of metabolic compensation and concomitant diabetic nephropathy].
The experimental and clinical researches suggest important role of advanced glycation end products (AGE) and oxidative stress in pathogenesis of diabetic angiopathy also including nephropathy. It is especially pointed at by the research done on inadequately controlled long-term diabetes. The aim of the study was to asses the total antioxidant capacity of plasma as well as the concentration of low-molecular antioxidants in plasma in diabetes type 2 patients in different stage of metabolic compensation and concomitant diabetic nephropathy.


METHODS
91 diabetes type 2 patients took part in the research (31 patients with metabolic compensated diabetes, 14 patients with metabolically decompensated diabetes, 14 patients with incipient and 16 patients with overt nephropathy). The control group consisted of 40 healthy men. The total antioxidant capacity was fluorometrically marked by means of ficoerythryne. The concentration of low-molecular antioxidants in plasma was marked on the basis of the time of free radicals scavenging by means of Trolox (6-hydroxy-2, 5,7, 8-tetramethethylchroman-2-carboxylic acid) of known concentration.


RESULTS
In patients with diabetes type 2 the total biggest antioxidant capacity was noted in metabolically compensated diabetes group and the total lowest antioxidant capacity was noted in diabetic patients with overt nephropathy. The highest mean concentration of low-molecular antioxidants was noted in metabolically compensated diabetes group and the lowest in diabetic patients with overt nephropathy. These differences were statistically significant.


CONCLUSIONS
In patients with diabetic nephropathy with the development of the disease significant insufficiency of plasma antioxidant barrier is noted. Diminishing of the total antioxidant capacity and depletion of plasma antioxidants noted in diabetes type 2 patients, especially inadequately metabolically controlled may constitute the essential pathogenetic factor of vascular complication in diabetes.
Błaszczak
Robert
R
Oddzial Kliniczny Kardiodiabetologii Szpitala Klinicznego nr w łodz.
Kujawski
Krzysztof
K
Kedziora-Kornatowska
Kornelia
K
Kornatowski
Tomasz
T
Kedziora
Józef
J
Szadujkis-Szadurski
Leszek
L
Markuszewski
Leszek
L
Rysz
Jacek
J
Ertel
Danuta
D
Olszewski
Robert
R
D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003928:Diabetic Nephropathies / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
D000975:Antioxidants