[Lymphocyte populations and subpopulations in peripheral blood of pregnant women with pre-eclampsia].
OBJECTIVE
To investigate the changes of lymphocyte populations and subpopulations in peripheral blood of pregnant women suffering preeclampsia.


METHODS
By means of flowcytometry the following lymphocyte populations and subpopulations were determined in peripheral blood of 37 pregnant women, of whom 12 had preeclampsia and 25 had normal pregnancy pespectively: Total lymphocyte count CD19+ (B-lymphocytes), CD2+ (Total T-lymphocytes), CD3+ (Immunocompetent T-lymphocytes) CD3+CD4+ (T-helpers), CD3+CD8+ (Suppressor-cytoxic T-lymphocytes), CD4+/CD8+ ratio, Lymphocyte subpopulations with predominant killer activity: CD8-CD56+, CD8+CD56+ and activated immunocompetent T-lymphocytes (CD3+HLADR+).


RESULTS
In patients with preeclampsia a T-helper increase and relative T-suppressor cytotoxic decrease was found. A significant increase of activated immunocompetent T-lymphocyte was found, compared to the total increase of immunocompetent T cell. No significant difference between cytotoxic cell with MHC unrestrained function (CD8-CD56+) and cell with MHC restrained cytotoxic function (CD8+CD56+) was found.


CONCLUSIONS
In pregnant women with preeclampsia the main lymphocyte populations and the immunocompetent T-lymphocytes are increased. The raised level of T-lymphocytes is found as a result of the substantial quantitative changes of T-helper cells an increase of CD4+/CD8+ ratio is prominent, which is a proof of the activated immune potential in pregnant women with preeclampsia with the dominant influence of T-helper subpopulations.
Dimitrakova-Dzhambazova
E
E
Milchev
N
N
Pavlov
P
P
Dimitrakov
D
D
D015703:Antigens, CD / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D018801:CD2 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D011225:Pre-Eclampsia / Q000097:blood* / Q000276:immunology*; D011247:Pregnancy / Q000097:blood* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D015703:Antigens, CD; D018941:Antigens, CD19; D018801:CD2 Antigens; D017252:CD3 Complex; D015704:CD4 Antigens; D019002:CD56 Antigen; D016827:CD8 Antigens; D006684:HLA-DR Antigens