Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons.
The choroid plexus epithelial (modified ependymal) cells (CPECs) are specialized for cerebrospinal fluid (CSF) production and serve as blood-CSF barrier. It is suggested that, in addition to CSF production, the CPECs may regulate CNS function through expression of secretory factors into CSF. There have been reports that the CPECs express various types of factors including growth factors. However, the actual effects of the molecules produced and secreted from the CPECs on the central nervous system (CNS) are virtually unknown both in vivo and in vitro. With the use of pure culture of CPECs, we demonstrated that the conditioned medium (CM) from CPECs can enhance neurite outgrowth and survival of cultured neurons derived from rat hippocampus on postnatal day 1 in 24-h cultures. The effect of the CM was retained in fractions that contains complex of molecules larger than 50kDa in native condition with ultrafiltration method and disappeared by trypsin digestion. The results of the present study indicate that CPECs can support the survival and function of neurons in vitro by secreting factors that are likely to be of peptide/protein nature rather than small chemicals.
Watanabe
Yumi
Y
Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoe-chou, Sakyo-ku, Kyoto 606-8501, Japan. ywatanabe@anat2.med.kyoto-u.ac.jp
Matsumoto
Naoya
N
Dezawa
Mari
M
Itokazu
Yutaka
Y
Yoshihara
Tomoyuki
T
Ide
Chizuka
C
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology* / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004847:Epithelial Cells / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D020287:In Situ Nick-End Labeling / Q000379:methods; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008297:Male; D009414:Nerve Growth Factors / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D017077:Culture Media, Conditioned; D007166:Immunosuppressive Agents; D009414:Nerve Growth Factors; D003561:Cytarabine; D005346:Fibroblast Growth Factors; D017228:Hepatocyte Growth Factor; D007335:Insulin-Like Growth Factor II; D014357:Trypsin; C073922:neurotrophin 4