title: Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E.
abstract: The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.
authors.affiliation: Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Bone S F
authors.initials: BS
authors.lastname: Tang
authors.forename: Kwok-Hung
authors.initials: KH
authors.lastname: Chan
authors.forename: Vincent C C
authors.initials: VC
authors.lastname: Cheng
authors.forename: Patrick C Y
authors.initials: PC
authors.lastname: Woo
authors.forename: Susanna K P
authors.initials: SK
authors.lastname: Lau
authors.forename: Clarence C K
authors.initials: CC
authors.lastname: Lam
authors.forename: Tsun-Leung
authors.initials: TL
authors.lastname: Chan
authors.forename: Alan K L
authors.initials: AK
authors.lastname: Wu
authors.forename: Ivan F N
authors.initials: IF
authors.lastname: Hung
authors.forename: Suet-Yi
authors.initials: SY
authors.lastname: Leung
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D003139:Common Cold / Q000235:genetics*; D028941:Coronavirus 229E, Human / Q000235:genetics / Q000502:physiology*; D018352:Coronavirus Infections / Q000235:genetics*; D005796:Genes / Q000235:genetics; D006801:Humans; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000502:physiology*; D045169:Severe Acute Respiratory Syndrome / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger