[Sensitivity of survivin-positive primary leukemia cells to chemotherapy].
To explore the sensitivity of survivin-positive primary acute leukemia (AL) cells to various chemotherapeutics, RT-PCR was used to detect the expression of survivin mRNA in AL cells, the cytotoxicity of chemotherapeutics was investigated with the MTT assay. The results indicated that for the primary AL cells cultured with various chemotherapeutics for 15 hours, the cytotoxicities of daunorubicin (DNR), homoharringtonine (HHT), aclacinomycin (Acla), Ara C (cytosine arabinoside), HA (HHT + Ara C), DA (DNR+Ara C) to the survivin-positive AL cells were similar with that to the survivin-negative AL cells. When AL cells were treated with AA (Acla + Ara C) in vitro, the cytotoxicity in survivin-positive group was apparently higher than that in survivin-negative group [(37.24 +/- 18.36)% vs (24.32 +/- 9.33)%, P = 0.032]. It is suggested that the survivin-positive AL cells may be sensitive to some chemotherapy, such as AA (Acla + Ara C).
Meng
Xiao-Li
XL
The Department of Hematology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. wyhai@zzu.edu.cn
D015250:Aclarubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D003561:Cytarabine / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007938:Leukemia / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin; C011157:aclacinomycins; D003561:Cytarabine; D015250:Aclarubicin