Separation of white blood cells from erythrocytes on a dielectrophoresis (DEP) based 'Lab-on-a-chip' device.
The 'Lab-on-a-chip technology' involves miniaturization of complex analytical procedures and is expected to enable laboratory testing to move from the central laboratory employing complex equipment into non-laboratory settings. We report the application of a printed circuit board (PCB)-based chip, generating dielectrophoretic (DEP)-based cylinder-shaped cages for separation and recovery of white blood cells from erythrocytes. This possibility is of interest to develop low-cost Lab-on-a-chip devices for diagnostic purposes. Accordingly, we demonstrate that white blood cells recovered from this Lab-on-a-chip device are suitable for PCR-based molecular diagnosis procedures employing DNA sequencing or biospecific interaction analysis using surface plasmon resonance and biosensor technology.
Borgatti
Monica
M
Laboratory for the Development of Pharmacological and Pharmacogenomic Therapy of Thalassemia, Biotechnology Center, Italy.
Altomare
Luigi
L
Baruffa
Martina
M
Fabbri
Enrica
E
Breveglieri
Giulia
G
Feriotto
Giordana
G
Manaresi
Nicoló
N
Medoro
Gianni
G
Romani
Aldo
A
Tartagni
Marco
M
Gambari
Roberto
R
Guerrieri
Roberto
R
D015374:Biosensing Techniques; D002452:Cell Count; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D019411:Clinical Laboratory Techniques / Q000295:instrumentation*; D003198:Computer Simulation; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D020014:K562 Cells; D007962:Leukocytes / Q000166:cytology*; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction; D020349:Surface Plasmon Resonance