title: Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct.
abstract: In this study, we have examined the presence and the distribution of receptors for leptin (Ob-R) in the oviduct of rabbits, and the effects of leptin on the release of prostaglandin (PG) F2alpha and PGE2 and on the activity of nitric oxide (NO) synthase (NOS) by oviducts cultured in vitro. Rabbits were killed during the follicular phase and the oviducts were incubated in vitro with leptin, PGF2alpha, PGE2, NO donor and inhibitors of NOS and cyclo-oxigenase (COX). Using immunohistochemistry, Ob-R-like positive reaction was observed only in the cytoplasm of secretory cells, having stronger intensity in the infundibulum and ampulla tracts than in the isthmus. Both leptin and NO donor inhibited PGE2 release, whereas they enhanced PGF2alpha release; NOS inhibitor alone or with leptin increased PGE2 and decreased PGF2alpha production; NOS activity was enhanced by leptin, while PGs did not affect this enzyme. This study suggests that the oviduct could be a potential target for endocrine regulation by leptin, whose circulating levels may act as a metabolic signal modulating oviductal PG release through mediation of the NOS/NO system.
authors.affiliation: Dipartimento di Biologia Molecolare, Cellulare e Animale, Università di Camerino, via F Camerini 1, 62032 Camerino, Italy. massimo.zerani@unicam.it
authors.forename: M
authors.initials: M
authors.lastname: Zerani
authors.forename: C
authors.initials: C
authors.lastname: Boiti
authors.forename: C
authors.initials: C
authors.lastname: Dall'Aglio
authors.forename: L
authors.initials: L
authors.lastname: Pascucci
authors.forename: M
authors.initials: M
authors.lastname: Maranesi
authors.forename: G
authors.initials: G
authors.lastname: Brecchia
authors.forename: C
authors.initials: C
authors.lastname: Mariottini
authors.forename: G
authors.initials: G
authors.lastname: Guelfi
authors.forename: D
authors.initials: D
authors.lastname: Zampini
authors.forename: A
authors.initials: A
authors.lastname: Gobbetti
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D020738:Leptin / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D009924:Organ Culture Techniques; D010057:Oviducts / Q000737:chemistry / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism*; D054411:Receptors, Leptin
chemical_list: D016861:Cyclooxygenase Inhibitors; D020738:Leptin; D020030:Nitric Oxide Donors; D011453:Prostaglandins; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D009599:Nitroprusside; D015237:Dinoprost; D019001:Nitric Oxide Synthase; D015232:Dinoprostone; D001241:Aspirin; D019331:NG-Nitroarginine Methyl Ester