title: In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE.
abstract: Both gram-negative and gram-positive bacteria possess protein tyrosine phosphatases (PTPs) with a catalytic Cys residue. In addition, many gram-positive bacteria have acquired a new family of PTPs, whose first characterized member was CpsB from Streptococcus pneumoniae. Bacillus subtilis contains one such CpsB-like PTP, YwqE, in addition to two class II Cys-based PTPs, YwlE and YfkJ. The substrates for both YwlE and YfkJ are presently unknown, while YwqE was shown to dephosphorylate two phosphotyrosine-containing proteins implicated in UDP-glucuronate biosynthesis, YwqD and YwqF. In this study, we characterize YwqE, compare the activities of the three B. subtilis PTPs (YwqE, YwlE, and YfkJ), and demonstrate that the two B. subtilis class II PTPs do not dephosphorylate the physiological substrates of YwqE.
authors.affiliation: Microbial Physiology and Genetics Group, BioCentrum, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Ivan
authors.initials: I
authors.lastname: Mijakovic
authors.forename: Lucia
authors.initials: L
authors.lastname: Musumeci
authors.forename: Lutz
authors.initials: L
authors.lastname: Tautz
authors.forename: Dina
authors.initials: D
authors.lastname: Petranovic
authors.forename: Robert A
authors.initials: RA
authors.lastname: Edwards
authors.forename: Peter Ruhdal
authors.initials: PR
authors.lastname: Jensen
authors.forename: Tomas
authors.initials: T
authors.lastname: Mustelin
authors.forename: Josef
authors.initials: J
authors.lastname: Deutscher
authors.forename: Nunzio
authors.initials: N
authors.lastname: Bottini
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D017027:Protein Tyrosine Phosphatases