title: Differential antibody responses to Plasmodium falciparum glycosylphosphatidylinositol anchors in patients with cerebral and mild malaria.
abstract: Glycosylphosphatidylinositol (GPI) membrane anchors of Plasmodium falciparum surface proteins are thought to be important factors contributing to malaria pathogenesis, and anti-GPI antibodies have been suggested to provide protection by neutralizing the toxic activity of GPIs. In this study, IgG responses against P. falciparum GPIs and a baculovirus recombinant MSP1p19 antigen were evaluated in two distinct groups of 70 patients each, who were hospitalized with malaria. Anti-GPI IgGs were significantly lower in patients hospitalized with confirmed cerebral malaria compared to those with mild malaria (P < 0.01) but did not discriminate for fatal outcome. In contrast, a specific marker of the anti-parasite immunity, as monitored by the anti-MSP1p19 IgG response, was similar in both cerebral and mild malaria individuals, although it was significantly lower in a subgroup with fatal outcomes. These results are consistent with a potential anti-toxin role for anti-GPI antibodies associated with protection against cerebral malaria.
authors.affiliation: Unité d'Immunologie, Institut Pasteur de Dakar, 36 Av. Pasteur, BP-220, Dakar, Senegal. perraut@pasteur.sn
authors.forename: Ronald
authors.initials: R
authors.lastname: Perraut
authors.forename: Bacary
authors.initials: B
authors.lastname: Diatta
authors.forename: Laurence
authors.initials: L
authors.lastname: Marrama
authors.forename: Olivier
authors.initials: O
authors.lastname: Garraud
authors.forename: Ronan
authors.initials: R
authors.lastname: Jambou
authors.forename: Shirley
authors.initials: S
authors.lastname: Longacre
authors.forename: Gowdahalli
authors.initials: G
authors.lastname: Krishnegowda
authors.forename: Alioune
authors.initials: A
authors.lastname: Dieye
authors.forename: D Channe
authors.initials: DC
authors.lastname: Gowda
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017261:Glycosylphosphatidylinositols / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016779:Malaria, Cerebral / Q000276:immunology* / Q000469:parasitology; D016778:Malaria, Falciparum / Q000276:immunology* / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000276:immunology; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000913:Antibodies, Protozoan; D017261:Glycosylphosphatidylinositols; D007074:Immunoglobulin G; D020066:Merozoite Surface Protein 1