ATP potentiates the formation of AChR aggregate in the co-culture of NG108-15 cells with C2C12 myotubes.
The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. In the co-cultures, antagonized P2Y(1) receptors showed a reduction in NG108-15 cell-induced AChR aggregation. Parallel to this observation, cultured NG108-15 cell secreted ATP into the conditioned medium in a time-dependent manner. Enhancement of ATP release from the cultured NG108-15 cells by overexpression of active mutants of small GTPases increased the aggregation of AChRs in co-culturing with C2C12 myotubes. In addition, ecto-nucleotidase was revealed in the co-culture, which rapidly degraded the applied ATP. These results support the notion that ATP has a role in directing the formation of post-synaptic apparatus in vertebrate neuromuscular junctions.
Ling
Karen K Y
KK
Department of Biology and Molecular Neuroscience Center, The Hong Kong University of Science and Technology, China.
Siow
Nina L
NL
Choi
Roy C Y
RC
Tsim
Karl W K
KW
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D020836:Protein Structure, Quaternary / Q000187:drug effects; D051381:Rats; D011950:Receptors, Cholinergic / Q000737:chemistry* / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058472:Receptors, Purinergic P2Y1
C546383:P2ry1 protein, mouse; C546384:P2ry1 protein, rat; D011950:Receptors, Cholinergic; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D000255:Adenosine Triphosphate; D020559:Monomeric GTP-Binding Proteins