[Cell apoptosis and the change of Fas/FasL gene in experimental pulmonary fibrosis].
OBJECTIVE
To investigate the changes and significance of cell apoptosis and Fas/FasL gene in pulmonary fibrosis.


METHODS
Forty mice were divided into two groups randomly, each group contained twenty mice. TUNEL, immunohistochemistry and in situ hybridization were used to detect the change of cell apoptosis, Fas/FasL mRNA and protein in mice with pulmonary fibrosis caused by bleomycin.


RESULTS
The apoptosis index of lung cells in the pulmonary fibrosis group (55.3 +/- 12.2) was higher than that of control group (4.7 +/- 1.0, t = 13.06, < 0.01). The expression of Fas/FasL mRNA (175.8 +/- 21.6, 5.2 +/- 1.6) and protein (956 +/- 96, 285 +/- 76) in the pulmonary fibrosis group was higher than that of control group (mRNA: 26.6 +/- 1.9, 0.5 +/- 0.4, t = 21.7, 8.79, all < 0.01; protein: 491 +/- 96, 100 +/- 18, t = 5.03, 12.81, < 0.01).


CONCLUSIONS
The apoptosis index of lung cells, Fas/FasL genes and protein were up-regulated in lung tissue of pulmonary fibrosis, which may play an important role in the development of disease.
Li
Jin-zhao
JZ
Institute of Respiratory Disease, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Li
Yan-jie
YJ
Li
Zhen-hua
ZH
Kang
Jian
J
Hou
Xian-ming
XM
Yu
Run-jiang
RJ
D000818:Animals; D017209:Apoptosis; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor