Spore-displayed streptavidin: a live diagnostic tool in biotechnology.
Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-streptavidin antibody was used for the verification of surface localization of expressed CotG-streptavidin fusion protein. FACS and dot-blot were used for the verification of biological activity of displayed streptavidin with FITC-labeled biotin.
Kim
June-Hyung
JH
Institute of Molecular Biology and Genetics, School of Chemical Engineering, Seoul National University, Republic of Korea.
Lee
Chang-Soo
CS
Kim
Byung-Gee
BG
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D005434:Flow Cytometry; D005822:Genetic Vectors; D008856:Microscopy, Fluorescence; D010447:Peptide Hydrolases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013171:Spores, Bacterial / Q000378:metabolism; D019809:Streptavidin / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C091352:cotG protein, Bacillus subtilis; D001710:Biotin; D019809:Streptavidin; D010447:Peptide Hydrolases