title: [Preparation and characterization of monoclonal antibodies against human RANTES molecule].
abstract: OBJECTIVE
  To prepare monoclonal antibodies against human RANTES molecule and identify the expression of RANTES in rat small intestine after small bowel transplantation.
  
  
  METHODS
  Murine mAbs were prepared by B lymphocyte hybridoma technique. The expression of RANTES in rat small intestine after small bowel transplantation was detected by immunohistochemistry.
  
  
  RESULTS
  Four hybridoma cell lines secreting monoclonal antibodies to human RANTES, FMU-RANTES 1, FMU-RANTES 2, FMU-RANTES 3 and FMU-RANTES 4, were established. The titers of a scetic mAbs reached to 1 x 10(-6) and the Ig subclass of FMU-RANTES 1, FMU-RANTES 3 and FMU-RANTES 4 was IgG1(kappa) and that of FMU-RANTES 2 was IgG2b(kappa). Among these mAbs, FMU-RANTES 1, FMU-RANTES 2 and FMU-RANTES 3 could bind human RANTES protein in Western bolt. FMU-RANTES 1, FMU-RANTES 2 and FMU-RANTES 4 could be used in immunohistochemistry staining. Rat RANTES molecule could be detected in the cyto plasm of epithelial cells in rat small intestine after small bowel transplantation.
  
  
  CONCLUSIONS
  Four mAbs against RANTES molecule were prepared, which can provide a useful tool in research on the structure and function of RANTES molecule. High expression of RANTES may be involved in the rejection of allogeneic graft.
authors.affiliation: Department of Gastrointestinal Surgery, Fourth Military Medical University, Xi'an 710032, China. weichang@fmmu.edu.cn
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Kang
authors.forename: Wei-zhong
authors.initials: WZ
authors.lastname: Wang
authors.forename: Dong-li
authors.initials: DL
authors.lastname: Chen
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Can-sheng
authors.initials: CS
authors.lastname: Zhu
authors.forename: Jun-feng
authors.initials: JF
authors.lastname: Du
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000276:immunology* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007422:Intestines / Q000166:cytology; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5