title: The cell surface receptor G6b, a member of the immunoglobulin superfamily, binds heparin.
abstract: The G6b gene, located in the human Major Histocompatibility Complex, encodes a receptor of the immunoglobulin (Ig) superfamily. In this study, we show using a variety of techniques that the extracellular domain of the G6b protein, containing a single Ig-like domain, binds to heparin with high affinity. In an ELISA assay, this binding was displaceable with soluble heparin with an IC50 value of approximately 0.5 microg/ml. Other sulfated glycans showed weaker or no competition. The observed interaction between G6b and heparin is strongly salt dependent suggesting a mainly electrostatic interaction. Heparin might modulate the interaction of G6b with its as yet unidentified protein ligand.
authors.affiliation: MRC Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, UK.
authors.forename: Edwin C J M
authors.initials: EC
authors.lastname: de Vet
authors.forename: Stephen A B
authors.initials: SA
authors.lastname: Newland
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lyons
authors.forename: Begoña
authors.initials: B
authors.lastname: Aguado
authors.forename: R Duncan
authors.initials: RD
authors.lastname: Campbell
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005803:Genes, Immunoglobulin; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008024:Ligands; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives*; D012995:Solubility; D055672:Static Electricity
chemical_list: D007141:Immunoglobulin Fc Fragments; D008024:Ligands; C119883:MPIG6B protein, human; D011971:Receptors, Immunologic; C037405:heparin-sepharose; D006493:Heparin; D012685:Sepharose