title: Characterization of the uterine phenotype during the peri-implantation period for LIF-null, MF1 strain mice.
abstract: Leukemia inhibitory factor plays a major role in the uterus and in its absence embryos fail to implant. Our knowledge of the targets for LIF and the consequences of its absence is still very incomplete. In this study, we have examined the ultrastructure of the potential implantation site in LIF-null MF1 female mice compared to that of wild type animals. We also compared expression of proteins associated with implantation in luminal epithelium and stroma. Luminal epithelial cells (LE) of null animals failed to develop apical pinopods, had increased glycocalyx, and retained a columnar shape during the peri-implantation period. Stromal cells of LIF-null animals showed no evidence of decidual giant cell formation even by day 6 of pregnancy. A number of proteins normally expressed in decidualizing stroma did not increase in abundance in the LIF-null animals including desmin, tenascin, Cox-2, bone morphogenetic protein (BMP)-2 and -7, and Hoxa-10. In wild type animals, the IL-6 family member Oncostatin M (OSM) was found to be transiently expressed in the luminal epithelium on late day 4 and then in the stroma at the attachment site on days 5-6 of pregnancy, with a similar but not identical pattern to that of Cox-2. In the LIF-null animals, no OSM protein was detected in either LE or stroma adjacent to the embryo, indicating that expression requires uterine LIF in addition to a blastocyst signal. Fucosylated epitopes: the H-type-1 antigen and those recognized by lectins from Ulex europaeus-1 and Tetragonolobus purpureus were enhanced on apical LE on day 4 of pregnancy. H-type-1 antigen remained higher on day 5, and was not reduced even by day 6 in contrast to wild type uterus. These data point to a profound disturbance of normal luminal epithelial and stromal differentiation during early pregnancy in LIF-nulls. On this background, we also obtained less than a Mendelian ratio of null offspring suggesting developmental failure.
authors.affiliation: Faculty of Life Sciences, University of Manchester, 3.239 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Fouladi-Nashta
authors.forename: C J P
authors.initials: CJ
authors.lastname: Jones
authors.forename: N
authors.initials: N
authors.lastname: Nijjar
authors.forename: L
authors.initials: L
authors.lastname: Mohamet
authors.forename: A
authors.initials: A
authors.lastname: Smith
authors.forename: I
authors.initials: I
authors.lastname: Chambers
authors.forename: S J
authors.initials: SJ
authors.lastname: Kimber
mesh_terms: D000818:Animals; D015415:Biomarkers; D015815:Cell Adhesion Molecules / Q000378:metabolism; D010064:Embryo Implantation; D005260:Female; D006031:Glycosylation; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D053605:Leukemia Inhibitory Factor; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D011247:Pregnancy; D014599:Uterus / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D015850:Interleukin-6; D037102:Lectins; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse