title: Effect of monophosphoryl lipid A on antibody response to diphtheria toxin and its subunits.
abstract: Monophosphoryl lipid A (MPL) was evaluated for its ability to enhance the antibody response to diphtheria toxin and its fragment A and fragment B subunits. BALB/c mice were immunized subcutaneously with 1 Lf of diphtheria toxoid in the presence of 25 microg of MPL on days 0 and 14. Two weeks after the second immunization, sera were obtained from the mice and analysed for antibody response to diphtheria toxin and its subunits. A new ELISA method, developed in our laboratory, was used to measure antibody levels against the toxin, fragment A, and fragment B. It was observed that MPL significantly enhanced antibody responses to diphtheria toxin and its subunits. However, there was no statistical difference between anti-A and anti-B responses. The results indicated that MPL seems to be a potential candidate as an adjuvant for future diphtheria vaccine formulation.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Kayhan
authors.initials: K
authors.lastname: Caglar
authors.forename: Cemalettin
authors.initials: C
authors.lastname: Aybay
authors.forename: Haluk
authors.initials: H
authors.lastname: Ataoglu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D004165:Diphtheria / Q000097:blood / Q000517:prevention & control; D004167:Diphtheria Toxin / Q000008:administration & dosage / Q000276:immunology; D004168:Diphtheria Toxoid / Q000008:administration & dosage* / Q000276:immunology; D004353:Drug Evaluation, Preclinical; D005260:Female; D007114:Immunization; D007279:Injections, Subcutaneous; D008050:Lipid A / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid; D008050:Lipid A; D010446:Peptide Fragments; C029795:diphtheria toxin fragment A; C039171:diphtheria toxin fragment B; C048436:monophosphoryl lipid A