[Inhibition of invasion and multiplication of Toxoplasma gondii in human colonic epithelial cells by a monoclonal antibody against protein SAG2].
By an bromodeoxyuridine (BrdU) incorporation assay, it was proved hat an IgG 1 subclass, murine monoclonal antibody to surface protein SAG2 of Toxoplasma gondii is capable of reducing the invasion and multiplication of the parasites in highly differentiated mucine secretory HT29-18N2 line cells from a human colon adenocarcinoma. This result shows the importance of surface protein SAG2 of T.gondii in invasion and further multiplication of parasites in the host cell.
Osorio
J C
JC
Universidad del Quindio, Carrera 15 Calle 12 Norte. Armenia, Quindio, Colombia. jhonc@multi.net.co
Sánchez
R M
RM
Iraola
R C
RC
Pérez
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000953:Antigens, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000469:parasitology; D019073:HT29 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000469:parasitology*; D051379:Mice; D015800:Protozoan Proteins / Q000276:immunology*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma