Nucleic acid isolation from ecological samples--fungal associations, lichens.
Ecological samples of fungal associations pose particular challenges for nucleic acid extraction due to the presence of several genomes. Thorough examination of the samples prior to extraction is important to assess the risks of contamination. If manual separation of symbionts or their axenic cultivation is not feasible, symbiont-specific primers can be applied in PCR experiments. A basic protocol is suggested here which can be optimized for specific applications.
Grube
Martin
M
Institute of Plant Sciences, Karl-Franzens-University Graz, 8010 Graz, Austria.
D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification*; D008011:Lichens / Q000382:microbiology*; D008722:Methods; D009696:Nucleic Acids / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal; D009696:Nucleic Acids