New perfluorinated polycationic dimerizable detergents for the formulation of monomolecular DNA nanoparticles and their in vitro transfection efficiency.
We describe the synthesis of new perfluorinated dimerizable detergents which contain a tricationic or tetracationic (linear or branched spermine, respectively) polar head, and report on their cmc, their ability to condense DNA into cationic monomolecular DNA nanoparticles as well as on the in vitro transfection efficiency of these nanoparticles. Such cationic nanoparticles were prone to display efficient cell transfection properties as a result of increased contact to the anionic cell surface and internalization by endocytosis, low size compatible with improved intracellular diffusion and nuclear pore crossing, and the presence of amine function of low pK(a) for their endosomal escape. The challenge was to design polymerizable polycationic detergents that display a cmc high enough for the monomer to perform monomolecular DNA condensation (as cationic particles) and low enough for the dimer to form stable nanoparticles capable of efficient cell transfection. Although we succeeded in formulating small-sized cationic monomolecular DNA nanoparticles (<40 nm) with these dimerizable perfluorinated spermine-based detergents for N/P ratios of up to 5 (N=number of detergent amine equivalents/P=number of DNA phosphate equivalents), these small-sized cationic nanoparticles proved to be poor non-specific transfection agents in vitro, even in the presence of chloroquine. Their poor transfection potential could be due more likely to Brownian motion which prevents these very small-sized particles from sedimentation and adsorption onto the adherent cell monolayer, and, consequently, from proteoglycan-triggered endocytosis.
Fabio
Karine
K
Laboratoire de Chimie Bioorganique, UMR 6001 CNRS, Université de Nice Sophia-Antipolis, Faculté des Sciences, 06108 Nice Cédex 2, France.
Di Giorgio
Christophe
C
Vierling
Pierre
P
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D019281:Dimerization; D051379:Mice; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry; D013096:Spermine / Q000031:analogs & derivatives*; D014162:Transfection
D003902:Detergents; D013096:Spermine; D004247:DNA