[The immediate early genes expression in the PC12 cells activated by anatoxin-a].
OBJECTIVE
To explore the regulative action of IEGs when the nicotinic acetylcholine receptors (nAChRs) of PC12 cells were activated by anatoxin-a.


METHODS
Using the reverse transcription polymerase chain reaction (RT-PCR) method, the mRNA gene expression of c-fos, c-jun, NGFI-A and NGFI-B were measured while PC12 cells were activated by anatoxin-a.


RESULTS
As 10(-9), 10(-8) and 10(-7) mol/L anatoxin-a activated PC12 cells for an hour, or 10(-7) mol/ L anatoxin-a activated PC12 cells for 30 min, 60 min, 120 min, the intracellular gene expression of c-fos and NGFI-A increased significantly than the control group (P < 0.05), it was 2-6 times than the control group. And the gene expression of c-fos presented dose-response and time-effect relation. However, under the same condition, the gene expression of c-jun and NGFI-B did not show any remarkable changes.


CONCLUSIONS
c-fos and NGFI-A might be involved to modulation the action of anatoxin-a activating the nAChRs of PC12 cells.
Zhang
Zhi-Hong
ZH
School of Public Health, Fudan University, Shanghai 200032, China. hong8759@sina.com
Qu
Wei-Dong
WD
Zhu
Hui-Gang
HG
D000818:Animals; D000087522:Cyanobacteria Toxins; D005786:Gene Expression Regulation / Q000187:drug effects*; D017781:Genes, Immediate-Early / Q000187:drug effects*; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D016716:PC12 Cells; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects*; D014326:Tropanes / Q000494:pharmacology*
D000087522:Cyanobacteria Toxins; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D014326:Tropanes; C509783:anatoxin a