Biotin synthase: enzyme or reactant?
Biotin synthase catalyzes formation of a thiophane ring through a radical mechanism that is difficult to reconstitute in vitro. Choi-Rhee and Cronan measure a turnover of 20-60 equivalents of biotin in vivo, but also find that turnover renders the protein susceptible to proteolytic destruction.
Jarrett
Joseph T
JT
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
D001710:Biotin / Q000096:biosynthesis / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D013466:Sulfurtransferases / Q000737:chemistry* / Q000378:metabolism
D001710:Biotin; D013466:Sulfurtransferases; C057985:biotin synthetase