Liquid chromatographic method for quantitation of patulin at 10 ng/mL in apple-based products intended for infants: interlaboratory study.
An interlaboratory trial for the determination of patulin in apple juice and fruit puree was conducted, involving 17 participants representing a cross section of industry, official food control, and research facilities. Mean recoveries reported ranged from 74 (10 ng/g) to 62% (25 ng/g) for apple juice and from 72 (25 ng/g) to 74% (10 ng/g) for fruit puree. Based on results for spiked samples (blind pairs at 2 levels), as well as naturally contaminated samples (blind pairs at 3 levels), the relative standard deviation for repeatability (RSDr) in juice ranged from 8.0 to 14.3% and in puree from 3.5 to 9.3%. The relative standard deviation for reproducibility (RSD(R)) in juice ranged from 19.8 to 39.5% and in puree from 12.5 to 35.2%, reflecting HORRAT values from 0.6 to 1.0 for juice and 0.4 to 0.9 for puree. The method showed acceptable within-laboratory and between-laboratory precision for each matrix, as required by current European legislation.
Arranz
Isabel
I
Institute for Reference Materials and Measurements, Food Safety and Quality Unit, European Commission-Joint Research Centre, Retieseweg 111, B-2440 Geel, Belgium.
Derbyshire
Michelle
M
Kroeger
Katy
K
Mischke
Carsten
C
Stroka
Joerg
J
Anklam
Elke
E
D001628:Beverages / Q000032:analysis; D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002273:Carcinogens; D007202:Indicators and Reagents; D012996:Solutions; D010365:Patulin