title: In vitro stimulation of penetration gland emptying by Trichobilharzia szidati and T. regenti (Schistosomatidae) cercariae. Quantitative collection and partial characterization of the products.
abstract: Induction of penetration gland emptying by cercariae of the bird schistosomes Trichobilharzia szidati and T. regenti employing linoleic acid, linolenic acid, praziquantel and calcium ionophore A23187 showed that both postacetabular and circumacetabular cells released their content at chosen stimulant concentrations. The gland secretions consisted of soluble and insoluble parts. The former one adhering to the ground seemed to have different saccharide composition from the glands of Schistosoma mansoni. It bound labelled saccharides, thus exhibiting lectin-like activity. Protein profiles of the latter one were identical after stimulation by all four stimulants in T. szidati. The soluble secretions contained several proteolytic enzymes; 31 kDa and 33 kDa cysteine proteases were identified in E/S products of T. szidati and T. regenti, respectively. The circumacetabular glands contained a significant amount of calcium. Immunohistochemistry revealed that the origin of E/S products after in vitro stimulation is in both penetration glands and tegumental structures. No crossreactivity was observed between the bird schistosomes and a serum raised against S. mansoni elastase.
authors.affiliation: Department of Parasitology, Charles University, Vinicná 7, 12844, Prague 2, Czech Republic, mikes@natur.cuni.cz
authors.forename: Libor
authors.initials: L
authors.lastname: Mikes
authors.forename: Lenka
authors.initials: L
authors.lastname: Zìdková
authors.forename: Martin
authors.initials: M
authors.lastname: Kasný
authors.forename: Jan
authors.initials: J
authors.lastname: Dvorák
authors.forename: Petr
authors.initials: P
authors.lastname: Horák
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005088:Exocrine Glands / Q000187:drug effects / Q000378:metabolism*; D015801:Helminth Proteins / Q000737:chemistry / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000502:physiology; D066298:In Vitro Techniques; D008041:Linoleic Acids / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011223:Praziquantel / Q000494:pharmacology; D012551:Schistosomatidae / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000871:Anthelmintics; D015801:Helminth Proteins; D008041:Linoleic Acids; D008042:Linolenic Acids; D000001:Calcimycin; D011223:Praziquantel