A potential for tissue restrictive gene therapy in renal cell carcinoma using MN/CA IX promoter.
BACKGROUND
The expression of the MN/carbonic anhydrase IX (CA IX) protein is detected in renal cell carcinoma (RCC). A tumor-specific MN promoter was designed and evaluated.


METHODS
Western blot, reverse transcription-PCR and Northern blot for MN expression were performed on Hela cells, LNCaP and four human RCC cell lines. The MN promoter (554 bp) was cloned into pGL3 luciferase reporter vectors and their activities were tested.


RESULTS
Hela cells, SKRC-31 and SKRC-38 expressed high levels of MN RNA and MN protein. The MN promoter has 5- to 10-fold higher activity in MN-positive cell lines than in MN-negative cell lines. It could enhance the activity of the basic SV40 early promoter (6 to 25 fold) in MN-positive cells but not in MN-negative cells.


CONCLUSIONS
MN expression in RCC may derive from altered expression of transcriptional factor(s), which binds to the MN enhancer element, and can be used for tumor specific toxic gene therapy for RCC.
Ou
Yen-Chuan
YC
Division of Urology, Department of Surgery, Taichung Veterans General Hospital, National Yang-Ming University School of Medicine, Taiwan. ycou@vghtc.vghtc.gov.tw
Gardner
Thomas A
TA
Kao
Chinghaj
C
Zhau
Haiyen E
HE
Chung
Leland W
LW
D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000096:biosynthesis / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; D008156:Luciferases; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases