[The expression of p21(WAF1/CIP1) and PCNA in hypopharyngeal and laryngeal squamous cell carcinomas].
OBJECTIVE
ln order to evaluate the significance of the expression of p21(WAF1/CIP1) and PCNA (proliferating cell nuclear antigen) in hypopharyngeal and laryngeal squamous cell carcinoma.


METHODS
Ninety-three patients who accepted operations for hypopharyngeal and laryngeal squamous cell carcinoma from 1998 to 2003 were included in this study, 45 cases were hypopharyngeal cancer, 25 cases were supraglottic cancer, 23 cases were glottic cancer. The clinical and histopathological features of these patients were reviewed. The Envision immunohistochemistry method was utilized to stain these faculties.


RESULTS
The expression of p21(WAF1/CIP1) had no correlate with differentiation, N stage, metastasis, survival, recurrence and laryngeal site of involvement. However, significantly correlated with T stage of hypopharyngeal and laryngeal squamous cell carcinoma, also correlated with prognosis of hypopharyngeal and laryngeal squamous cell carcinoma. The percentage of PCNA labeled cells showed the statistically significant with clinical and N stage, but no significance was found among other clinicopathological parameter and PCNA staining intensity.


CONCLUSIONS
p21(WAF1/CIP1), PCNA play important roles in the progression of hypopharyngeal and laryngeal squamous cell carcinoma and are possible prognostic discriminators in hypopharyngeal and laryngeal squamous cell carcinoma.
Li
Xiaoyan
X
Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai 200080, China.
Tadashi
Nakashima
N
Dong
Pin
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018809:Proliferating Cell Nuclear Antigen