title: Biological characteristics of cultured cells derived from various types of human brain tumors.
abstract: We placed in culture brain tumors from 45 cases (7 cases of astrocytoma, 2 from oligodendrogliomas, 2 glioblastomas, 2 ependymomas, 13 meningiomas, 6 pituitary adenomas, 5 neurinomas, a malignant lymphoma, a choroid plexus papilloma, and 6 metastatic tumors) and succeeded in making a primary culture from 33, and maintained 17 in vitro over a considerable period of time (greater than three months). In the early period of the primary cultures, the astrocytoma cells had cytoplasmic processes which contacted each other, the oligodendroglioma cells were small and spindle-shaped, the glioblastoma cells were neoplastic with pleopmorphic features and possessed cytoplasmic processes, the ependymoma cells formed a rosette-like cell arrangement, the meningioma cells were spindle- or round-shaped cells and characterized as forming psammoma bodies, the pituitary adenoma cells were round- or oval-shaped cells and produced growth hormone (GH), adenocorticoid tropic hormone (ACTH), prolactin, or other hypophyseal hormones, the choroid plexus papilloma cells were round-or polygonal and showed a papillary cell arrangement, the neurinoma cells were spindle- or fibrous-shaped cells, and the malignant lymphoma cells were round and formed cell aggregates floating in the culture medium.
authors.affiliation: Ishiwata Obstetrics & Gynecology Hospital, Mito-shi, Ibaraki-ken, Japan.
authors.forename: Isamu
authors.initials: I
authors.lastname: Ishiwata
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiwata
authors.forename: Megumi
authors.initials: M
authors.lastname: Iguchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Soma
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Sato
authors.forename: Makoto
authors.initials: M
authors.lastname: Sonobe
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000473:pathology* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D003593:Cytoplasm / Q000473:pathology; D004806:Ependymoma / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D005909:Glioblastoma / Q000473:pathology / Q000648:ultrastructure; D013006:Growth Hormone / Q000096:biosynthesis; D006801:Humans; D008223:Lymphoma / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008577:Meningeal Neoplasms / Q000473:pathology; D008579:Meningioma / Q000473:pathology; D008853:Microscopy; D008875:Middle Aged; D009442:Neurilemmoma / Q000473:pathology / Q000648:ultrastructure; D009837:Oligodendroglioma / Q000473:pathology / Q000648:ultrastructure; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology; D011388:Prolactin / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013006:Growth Hormone