Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells.
Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, covering about 80% of the estimated genome of this dimorphic, thermo-regulated fungus. The data provide a comprehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in mycelium and yeast cells was detected, which was confirmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about sequences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets.
Felipe
Maria Sueli S
MS
Departamento de Biologia Celular, Universidade de Brasília, 70910-900, Brasília, DF, Brazil. msueli@unb.br
Andrade
Rosângela V
RV
Arraes
Fabrício B M
FB
Nicola
André M
AM
Maranhão
Andréa Q
AQ
Torres
Fernando A G
FA
Silva-Pereira
Ildinete
I
Poças-Fonseca
Márcio J
MJ
Campos
Elida G
EG
Moraes
Lídia M P
LM
Andrade
Patrícia A
PA
Tavares
Aldo H F P
AH
Silva
Simoneide S
SS
Kyaw
Cynthia M
CM
Souza
Diorge P
DP
Pereira
Maristela
M
Jesuíno
Rosália S A
RS
Andrade
Edmar V
EV
Parente
Juliana A
JA
Oliveira
Gisele S
GS
Barbosa
Mônica S
MS
Martins
Natália F
NF
Fachin
Ana L
AL
Cardoso
Renato S
RS
Passos
Geraldo A S
GA
Almeida
Nalvo F
NF
Walter
Maria Emília M T
ME
Soares
Célia M A
CM
Carvalho
Maria José A
MJ
Brígido
Marcelo M
MM
D015152:Blotting, Northern; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D016681:Genome, Fungal; D020407:Internet; D008954:Models, Biological; D008969:Molecular Sequence Data; D025282:Mycelium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010228:Paracoccidioides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic; D015854:Up-Regulation
D018076:DNA, Complementary; D012333:RNA, Messenger