[Study of immunological effect of dendritic cell transfected with survivin gene on the specific anti-alimentary tract tumor].
OBJECTIVE
To investigate the effects of dendritic cells (DCs) transfected with survivin gene, and to observe the effective and specific anti-tumor immunological effect induced by modified DC in vitro.


METHODS
Survivin gene was transfected to DCs with liposomes. Survivin expression could be detected both in DCs cells and in cell culture with method of Western blot. Cytokines as well as cellular surface molecule such as IL-12, TNF-alpha, CD1 alpha, CD83, MHCII, CD80 and CD86 were detected. The competence of inducing human specific cytotoxic T lymphocyte (CTLs) was also detected with MTT.


RESULTS
Survivin expression could be detected both in DCs which were transfected with survivin cDNA and in cell culture superior. The IL-12 and TNF-alpha level was (265.2 +/- 32.7), (437.1 +/- 83.5) pg/ml, and much higher in transgened DC cells than blank DC cells (P < 0.05). CD1 alpha, CD83, MHCII, CD80 and CD86 was high expressed in survivin-DC cells, however, it was low expressed in blank DC cells. The lyse rate to gastric cancer cell, colon cancer cell and bile duct cancer cell was 65%, 77%, and 85% respectively, and these were much higher than those of blank DC cells.


CONCLUSIONS
DCs transfected with survivin gene could induce specific cytotoxic T lymphocytes and strikingly raised DC cell's antigen present function, and have specific CTL killing activity.
Sun
Hua-wen
HW
Department of General Surgery, People Hospital, Wuhan University, Wuhan 430060, China. sxshwyq@sina.com
Tang
Cong
C
Tang
Qi-bin
QB
Zou
Sheng-quan
SQ
Qiu
Fa-zu
FZ
D015703:Antigens, CD / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D005770:Gastrointestinal Neoplasms / Q000628:therapy*; D006801:Humans; D016233:Immunotherapy, Active; D066298:In Vitro Techniques; D051034:Inhibitor of Apoptosis Proteins; D018664:Interleukin-12 / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D000077022:Survivin; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015703:Antigens, CD; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12