[Expressions of IL-18Ralpha and IL-18Rbeta on rat primary renal tubular epithelial cells].
OBJECTIVE
To detect the expressions of the IL-18Ralpha mRNA and IL-18Rbeta mRNA in primary rat renal tubular epithelial cells (RTECs).


METHODS
The culture of primary RTECs was performed by renal tubular segment sticking mass method. The cellular type was identified by immunocytochemical staining. The IL-18Ralpha mRNA and IL-18Rbeta mRNA expressions in RTECs were detected by RT-PCR.


RESULTS
The result of immunocytochemical staining proved that the cultured cells were RTECs, IL-18Ralpha mRNA and IL-18Rbeta mRNA were detected in RTECs.


CONCLUSIONS
IL-18Ralpha mRNA and IL-18Rbeta mRNA are expressed in RTECs, which provide the experimental basis for exploring IL-18R role in renal interstitial fibrosis.
Du
Sheng-hua
SH
Institute of Kidney Diseases, Guangdong Medical College, Zhanjiang 524023, China. dsh1205@126.com
Liu
Hua-feng
HF
Tang
De-shen
DS
Chen
Xiao-wen
XW
D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005355:Fibrosis / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D053727:Interleukin-18 Receptor alpha Subunit / Q000235:genetics*; D053728:Interleukin-18 Receptor beta Subunit / Q000235:genetics*; D007684:Kidney Tubules / Q000166:cytology* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D053727:Interleukin-18 Receptor alpha Subunit; D053728:Interleukin-18 Receptor beta Subunit; D012333:RNA, Messenger