title: Antinociceptive activity of Calotropis procera latex in mice.
abstract: This work evaluated the antinociceptive effect of proteins from the Calotropis procera (Asclepiadaceae) latex using three different experimental models of nociception in mice. The latex protein fraction administered intraperitoneally in male mice at the doses of 12.5, 25 and 50 mg/kg showed the antinociceptive effect in a dose dependent manner compared to the respective controls in all assays. Inhibitions of the acetic acid-induced abdominal constrictions were observed at the doses of 12.5 (67.9%), 25 (85%) and 50 (99.5%) mg/kg compared to controls. Latex protein at the doses of 25 (39.8%; 42%) and 50 mg/kg (66.6%; 99.3%) reduced the nociception produced by formalin in the 1st and 2nd phases, respectively, and this effect was not reversed by pretreatment with naloxone (1 mg/kg). In the hot plate test, an increase of the reaction time was observed only at 60 min after the treatment with latex at the doses of 25 (79.5%) and 50 (76.9%) mg/kg, compared to controls and naloxone was ineffective to reverse the effect. It was concluded that the protein fraction derived from the whole latex of Calotropis procera possesses antinociceptive activity, which is independent of the opioid system.
authors.affiliation: Laboratório de Neurofarmacologia, Extensão do Lab. de Farmacologia Molecular e Toxinologia, Núcleo de Toxinologia de Venenos Animais, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, 740-000 Fortaleza, CE, Brazil.
authors.forename: Paula Matias
authors.initials: PM
authors.lastname: Soares
authors.forename: Silvane R
authors.initials: SR
authors.lastname: Lima
authors.forename: Samara G
authors.initials: SG
authors.lastname: Matos
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Andrade
authors.forename: Manoel C A
authors.initials: MC
authors.lastname: Patrocínio
authors.forename: Cleverson D T
authors.initials: CD
authors.lastname: de Freitas
authors.forename: Márcio V
authors.initials: MV
authors.lastname: Ramos
authors.forename: David N
authors.initials: DN
authors.lastname: Criddle
authors.forename: Bruno A
authors.initials: BA
authors.lastname: Cardi
authors.forename: Krishnamurti M
authors.initials: KM
authors.lastname: Carvalho
authors.forename: Ana Maria S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Silvânia M M
authors.initials: SM
authors.lastname: Vasconcelos
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D031107:Calotropis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde; D006358:Hot Temperature; D007840:Latex / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010147:Pain Measurement / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D011930:Reaction Time / Q000187:drug effects
chemical_list: D000700:Analgesics; D007840:Latex; D009292:Narcotic Antagonists; D010940:Plant Proteins; D005557:Formaldehyde; D009270:Naloxone; D019342:Acetic Acid