[Immunophenotypes in 115 patients with acute myeloid leukemia by multi-color flow cytometry].
Immunophenotyping has become common in the diagnosis and classification of leukemia. To evaluate the immunophenotype of acute myeloid leukemia (AML), multiparameter flow cytometry and CD45/SSC gating were used to analyze the surface and cytoplasmic antigen expressions in 115 cases of AML. The results were compared with the French-American-British (FAB) Cooperative Group classification to help define the best use and role of multiparameter flow cytometry in the diagnosis and proper classification of AML. The results showed that CD38, CD38 and CD13 were the most commonly expressed antigen (94.8%, 91.3% and 89.6%, respectively). CD7 was the most commonly expressed lymphoid antigen (20.2%), followed by CD19 (16.5%) and CD2 (15%). Some immunophenotypes correlated with FAB type, including increased frequency of CD2 in M(3); lack of HLA-DR, CD34 and CD56 expression in M(3); increased frequency of CD19 in M(2), CD14 and CD56 in M(5) and lack of MPO in M(0). In conclusion, multiparameter flow cytometry is a reliable technique in the diagnosis of AML, and some immunophenotypes correlate with FAB type.
Wang
Xing-Bing
XB
Stem Cell Research and Application Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Yao
Jun-Xia
JX
Zheng
Jin-E
JE
Liu
Jun
J
Li
Xiao-Qing
XQ
He
Yan-Li
YL
Yu
Jing-Ming
JM
Yang
Jing
J
Wei
Jun
J
Liu
Zhong-Ping
ZP
Huang
Shi-Ang
SA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007223:Infant; D007951:Leukemia, Myeloid / Q000145:classification / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD