Demonstration of A antigen and A allele of ABO histo-blood group in nail in a case with the absence of A antigen and anti-A antibody in blood.
We report a case with the inconsistency that the red blood cells lacked both A- and B-antigens while the serum showed reactivity with control B-red cells but not with A-red cells. A- and B-antigens were examined by serological blood typing and immunohistochemical staining, and DNA analyses were performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), DNA sequencing, and hot-stop PCR. A-antigens were demonstrable in the nail of the subject by serological study and immunostaining. DNA analyses showed that the nail retained a small amount of A-allele comparing to that of O-allele. Those genomic analyses of ABO genes were useful for demonstration of A allele in the nail of an individual with the absence of A antigen on red blood cells and the corresponding antibody in serum.
Michino
Junko
J
Division of Transfusion Medicine, Toyama Medical and Pharmaceutical University Hospital, Toyama 930-0194, Japan.
Hata
Yukiko
Y
Matsui
Kazuhiro
K
Takizawa
Hisao
H
Kominato
Yoshihiko
Y
Tabata
Sachiyo
S
Nishino
Kazuma
K
Yasumura
Satoshi
S
Kitajima
Isao
I
D000017:ABO Blood-Group System / Q000235:genetics* / Q000276:immunology*; D000483:Alleles; D001788:Blood Grouping and Crossmatching; D002648:Child; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009262:Nails / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D000017:ABO Blood-Group System