title: Inhibitory effects of triterpenes isolated from Chuling (Polyporus umbellatus Fries) on free radical-induced lysis of red blood cells.
abstract: Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats.
authors.affiliation: Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama 0930-0194, Japan. s114@ms.toyama-mpu.ac.jp
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Sekiya
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hikiami
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Nakai
authors.forename: Iwao
authors.initials: I
authors.lastname: Sakakibara
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nozaki
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Kouta
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimada
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Terasawa
mesh_terms: D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008297:Male; D020072:Polyporales / Q000302:isolation & purification; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D014315:Triterpenes