title: Changes in soluble adhesion molecules in gingival crevicular fluid following periodontal surgery.
abstract: BACKGROUND
  Inflammation of periodontal tissues during postoperative wound healing is mediated by cell surface adhesion molecules. Soluble forms of these antigens have also been identified and shown to be important in immunoregulatory processes, but have previously not been investigated during periodontal repair and regeneration. The present study has examined the presence and possible changes in soluble intercellular adhesion molecule-1 (sICAM-1; CD54) and lymphocyte function-associated antigen-3 (sLFA-3; CD58) in gingival crevical fluid (GCF) following periodontal surgery.
  
  
  METHODS
  GCF samples were collected from four groups: 1) a guided tissue regeneration (GTR) test; 2) a GTR control, at least one complete tooth unit away from the periodontal defect; 3) a conventional flap (CF) surgery; and 4) a crown lengthening (CL). Sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of sICAM-1 and sLFA-3 in the GCF samples.
  
  
  RESULTS
  A marked increase in GCF volumes was found in all sites after surgery, although a persistent increase was associated only with the period of membrane retention at the GTR test sites. In addition, sICAM-1 and sLFA-3 were found in the GCF of healthy as well as diseased sites prior to treatment and the total amounts of both increased transiently following surgical intervention, especially sLFA-3. However, the concentrations of these GCF components, particularly sICAM-1, tended to decrease.
  
  
  CONCLUSIONS
  The temporal decrease in the concentration of sICAM-1 and sLFA-3 in GCF may serve to enhance inflammatory reactions at surgically-treated periodontal sites, thereby limiting repair and regeneration in the periodontium. These soluble adhesion molecules may thereby be of potential therapeutic value and might also be useful markers for monitoring periodontal wound healing.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Marmara University, Istanbul, Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Kuru
authors.forename: Alun C
authors.initials: AC
authors.lastname: Kirby
authors.forename: Gareth S
authors.initials: GS
authors.lastname: Griffiths
authors.forename: Aviva
authors.initials: A
authors.lastname: Petrie
authors.forename: Irwin
authors.initials: I
authors.lastname: Olsen
mesh_terms: D000328:Adult; D018968:CD58 Antigens / Q000032:analysis / Q000378:metabolism*; D016022:Case-Control Studies; D016556:Crown Lengthening; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000276:immunology; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism*; D008297:Male; D019647:Oral Surgical Procedures; D010510:Periodontal Diseases / Q000378:metabolism* / Q000601:surgery*; D012038:Regeneration / Q000502:physiology*; D013524:Surgical Flaps
chemical_list: D018968:CD58 Antigens; D018836:Inflammation Mediators; D018799:Intercellular Adhesion Molecule-1