title: Birth of a healthy baby after transfer of blastocysts derived from cryopreserved human oocytes fertilized with frozen spermatozoa.
abstract: OBJECTIVE
  To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University-based assisted reproduction center.
  
  
  METHODS
  A 37-year-old woman with secondary infertility of 4 years' duration.
  
  
  METHODS
  Retrieved oocytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing.
  
  
  METHODS
  Fertilization and embryo development to blastocyst stage, pregnancy, and outcome.
  
  
  RESULTS
  Ten of 14 frozen oocytes survived after thawing. Eight of them were fertilized by performing ICSI and three developed into 7- to 8-cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery of a healthy baby boy.
  
  
  CONCLUSIONS
  This case demonstrates the feasibility of inseminating the frozen-thawed human oocytes after 4 hours of in vitro culture and the zygotes derived from frozen oocytes and frozen spermatozoa can be cultured to blastocysts resulting in the live birth of a healthy baby boy.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin NT, Hong Kong SAR, China.
authors.forename: Grace Ching-Ching
authors.initials: GC
authors.lastname: Tjer
authors.forename: Tony Tak-Yu
authors.initials: TT
authors.lastname: Chiu
authors.forename: Lai-Ping
authors.initials: LP
authors.lastname: Cheung
authors.forename: Ingrid Hung
authors.initials: IH
authors.lastname: Lok
authors.forename: Christopher John
authors.initials: CJ
authors.lastname: Haines
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D050498:Live Birth; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D013094:Spermatozoa