Constitutive expression of human lactoferrin and its N-lobe in rice plants to confer disease resistance.
The milk protein, lactoferrin, is known to have antibacterial, antiviral, and antifungal activities. To explore the possibility of conferring disease resistance in plants by expressing this protein, the gene for the full-length human lactoferrin (HLF), as well as the N-lobe, the N-terminal half molecule (HLFN), was introduced into rice plants and expressed constitutively under the control of the cauliflower mosaic virus 35S promotor. Western blot analysis of leaves from HLF-transgenic rice plants showed an 80 kDa-band, which was about 1-2 kDa less than human milk lactoferrin. HLFN was expressed as a 45-kDa protein and retained its heparin-binding property. Deglycosylation experiments suggested that both proteins produced by the plants had plant-type oligosaccharide chains. The transgenic rice plants were assessed for resistance against disease-causing bacteria, virus, and fungi. Of the pathogens tested, significant resistance against Burkholderia (Pseudomonas) plantarii, the causative agent of bacterial seedling blight disease, was observed in the transgenic plants expressing HLF or HLFN.
Takase
Kenji
K
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. ktakase@nias.affrc.go.jp
Hagiwara
Kiyoshi
K
Onodera
Haruko
H
Nishizawa
Yaeko
Y
Ugaki
Masashi
M
Omura
Toshihiro
T
Numata
Shinichi
S
Akutsu
Katsuki
K
Kumura
Haruto
H
Shimazaki
Kei-ichi
K
D015153:Blotting, Western; D019117:Burkholderia / Q000187:drug effects / Q000254:growth & development; D017796:Caulimovirus / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D007781:Lactoferrin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D012087:Reoviridae / Q000187:drug effects / Q000254:growth & development; D014162:Transfection; D014170:Transformation, Genetic; D014974:Xanthomonas / Q000187:drug effects / Q000254:growth & development
D009844:Oligosaccharides; D006493:Heparin; D007781:Lactoferrin