title: A xylogalactofucan from the brown seaweed Spatoglossum schröederi stimulates the synthesis of an antithrombotic heparan sulfate from endothelial cells.
abstract: The brown seaweed Spatoglossum schröederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 microg) nor hemorrhagic activities (100 microg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 microg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound.
authors.affiliation: Laboratório de Glicobiologia, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Rio Grande do Norte-UFRN, Natal-RN, Brazil. hugo-alexandre@uol.com.br
authors.forename: Hugo Alexandre de Oliveira
authors.initials: HA
authors.lastname: Rocha
authors.forename: Luana Cristina Lins de Medeiros
authors.initials: LC
authors.lastname: Bezerra
authors.forename: Ivan Rui Lopes
authors.initials: IR
authors.lastname: de Albuquerque
authors.forename: Leandro Silva
authors.initials: LS
authors.lastname: Costa
authors.forename: Celina Maria Pinto
authors.initials: CM
authors.lastname: Guerra
authors.forename: Luiz Diz
authors.initials: LD
authors.lastname: de Abreu
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Edda Lisboa
authors.initials: EL
authors.lastname: Leite
mesh_terms: D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis*; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012624:Seaweed; D013917:Thrombin / Q000037:antagonists & inhibitors*
chemical_list: D000925:Anticoagulants; D006497:Heparan Sulfate; D010936:Plant Extracts; D013917:Thrombin