Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis.
We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem + monolith; microsystem + nib) and their test before the fabrication and testing of fully integrated microsystems.
Carlier
Julien
J
Université des Sciences et Technologies de Lille (Lille 1), Laboratoire de Chimie Organique et Macromoléculaire, UMR CNRS 8009, Bâtiment C4, 2eme etage, 59655 Villeneuve d'Ascq, France.
Arscott
Steve
S
Thomy
Vincent
V
Camart
Jean-Christophe
JC
Cren-Olivé
Cécile
C
Le Gac
Séverine
S
D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018511:Systems Integration