Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity.
Streptomyces aureofaciens CMUAc130 was isolated from the root tissue of Zingiber officinale Rosc. (Zingiberaceae). It was an antagonist of Colletotrichum musae and Fusarium oxysporum, the causative agents of anthracnose of banana and wilt of wheat, respectively. Evidence for the in vitro antibiosis of S. aureofaciens CMUAc130 was demonstrated by the zone of fungal-growth inhibition. Microscopic observations showed thickness and bulbous structures at the edges of the inhibited fungal hyphae. The culture filtrate and crude extract from this strain were all inhibitory to tested phytopathogenic fungi. The major active ingredients from the culture filtrate of S. aureofaciens CMUAc130 were purified by silica gel-column chromatography and identified to be (i) 5,7-dimethoxy-4-p-methoxylphenylcoumarin and (ii) 5,7-dimethoxy-4-phenylcoumarin by NMR and mass-spectral data, respectively. Bioassay studies showed that compounds (i) and (ii) had antifungal activities against tested fungi, and their MICs were found to be 120 and 150 microg ml(-1), respectively. This is the first report of compounds (i) and (ii) from micro-organisms as active ingredients for the control of phytopathogenic fungi.
Taechowisan
Thongchai
T
Department of Biology, Faculty of Science, Silpakorn University, Nakorn Pathom 73000, Thailand.
Lu
Chunhua
C
School of Life Sciences, Xiamen University, Xiamen 361005, China.
Shen
Yuemao
Y
Kunming Institute of Botany (KIB), The Chinese Academy of Sciences, Kunming 650204, China.
Lumyong
Saisamorn
S
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
D000898:Antibiosis; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020231:Colletotrichum / Q000187:drug effects* / Q000254:growth & development; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development; D020939:Zingiber officinale / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D028521:Musa / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D013304:Streptomyces aureofaciens / Q000254:growth & development / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology
D000935:Antifungal Agents; D003374:Coumarins; C030123:coumarin