Prevalence of cna, fnbA and fnbB adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to different types of implant.
Here are reported data on virulence determinants of Staphylococcus aureus from orthopedic surgical infections, emphasizing on the genes encoding fibronectin (fnbA, fnbB) and collagen (cna) adhesins. 191 S. aureus strains from orthopedic infections (53 from internal fixation devices, 29 external fixation devices, 15 knee arthroprostheses, 30 hip arthroprostheses, 45 surgical reconstruction and 19 non-associated to medical devices) were investigated for the presence of the genes of the collagen-binding protein Cna and of the two fibronectin-binding proteins, FnbA and FnbB. 87 (46%) strains were found to be cna+ without significant variations across the different surgical categories considered. Conversely, the fnbA and the fnbB genes were almost always present in all surgical categories. The finding that, among the investigated adhesins, fibronectin-adhesins are present in the majority of the implant associated S. aureus clinical isolates encourages the development of strategies to specifically block the interaction of bacteria with matrix fibronectin by antagonist ligands.
Arciola
Carla Renata
CR
Research Unit on Implant Infections, Rizzoli Orthopedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy. carlarenata.arciola@ior.it
Campoccia
Davide
D
Gamberini
Simonetta
S
Baldassarri
Lucilla
L
Montanaro
Lucio
L
D018829:Adhesins, Bacterial / Q000235:genetics*; D003094:Collagen / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D009985:Orthopedics; D016133:Polymerase Chain Reaction; D019736:Prostheses and Implants / Q000382:microbiology; D016459:Prosthesis-Related Infections / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D013530:Surgical Wound Infection / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; D037521:Virulence Factors; C048165:adhesin, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial; D003094:Collagen