[The effects of rBCG expressing Der p2 in the form of lipoprotein on murine immune response].
OBJECTIVE
To investigate the effects of rBCG vaccination containing foreign antigen Der p2 in the form of lipoprotein on murine immune response.


METHODS
6 to 8 weeks old and newborn BALB/c mice were vaccined intraperitoneally with 10(6) CFU rBCG or BCG. At the same time, the control group was injected with saline. Six weeks later, all animals were injected with Der p2 (20 microg). After two weeks later, the concentrations of IL-4 and IFN-gamma in the serum and splenocyte culture supernatant (STLCS) were determined by ELISA, and Th subgroups were determined by double fluorescent staining and flow cytometry.


RESULTS
After vaccination, the serum and STLCS from both rBCG-immunized and BCG-immunized group of adult and newborn BALB/c mice had significantly higher level of IFN-gamma and lower level of IL-4 than those from control groups. Besides, there was the larger percentage of CD4 (+) IFN-gamma (+) cells in spleen from rBCG-vaccined and BCG-vaccined mice than that from control group. However, the percentage of CD4 (+) IL-4 (+) cells in spleen cells from rBCG-vaccined and BCG-vaccined group was lower than that from control group. Moreover, the level of IFN-gamma in STLCS from rBCG-immunized was significantly higher, compared with that from BCG-immunized mice. At the same time, the percentage of CD4 (+) IFN-gamma (+) cells in spleen from rBCG-vaccined mice was larger than that from BCG-vaccined group.


CONCLUSIONS
Both rBCG and BCG could stimulate Th1 predominant immune response, when injected intraperitoneally into adult or newborn BALB/c mice, The Der p2 expressed on the cell wall of BCG can work as the component of BCG and be recognized by the immune system of mice, therefore stimulates Der p2-specific Th1 predominant immune response. These data indicate that recombinant BCG-expressing antigens can be used as the antigen-specific vaccines against allergic diseases by regulating the balance of Th1/Th2.
Shi
Jie-ran
JR
Department of Respiratory Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. sjr96698@163.net
Zhang
Xin-hai
XH
Shi
Chang-hong
CH
Cao
Yun-xin
YX
Wu
Chang-gui
CG
Li
Yuan
Y
Fan
Xiong-lin
XL
Qi
Hao-wen
HW
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D002473:Cell Wall / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D007114:Immunization; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D008074:Lipoproteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000166:cytology / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D039741:Antigens, Dermatophagoides; D008074:Lipoproteins; D015847:Interleukin-4; D007371:Interferon-gamma