[The effect of the extract from Ranunculus japonicus on [Ca2+]i inside rabbit VSMC by serologic pharmacological test].
OBJECTIVE
To observe the influence of serum containing the extract from Ranunculus japonicus (ERj) on the amount of [Ca2+]i inside vascular smooth muscle cells (VSMC).


METHODS
Serum containing ERj was prepared by serum pharmacological method. Cultured VSMCs induced by NE were treated with the serum, and the influence of it on the amount [Ca2+]i inside cells indicated with INDO-AM was determined.


RESULTS
The serum containing ERj could markedly decrease the increase of the amount of free [Ca2+]i induced by NE in cultured VSMC.


CONCLUSIONS
The serum containing ERj could decrease the intracellular [Ca2+]i.
Cai
Sa
S
Department of Pharmacy, Guangdong College of Pharmacy, Guangzhou 510224.
Li
Xiaokun
X
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005456:Fluorescent Dyes; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D031947:Ranunculus / Q000737:chemistry*; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D002118:Calcium