Use of in vivo-induced antigen technology for identification of Escherichia coli O157:H7 proteins expressed during human infection.
Using in vivo-induced antigen technology (IVIAT), a modified immunoscreening technique that circumvents the need for animal models, we directly identified immunogenic Escherichia coli O157:H7 (O157) proteins expressed either specifically during human infection but not during growth under standard laboratory conditions or at significantly higher levels in vivo than in vitro. IVIAT identified 223 O157 proteins expressed during human infection, several of which were unique to this study. These in vivo-induced (ivi) proteins, encoded by ivi genes, mapped to the backbone, O islands (OIs), and pO157. Lack of in vitro expression of O157-specific ivi proteins was confirmed by proteomic analysis of a mid-exponential-phase culture of E. coli O157 grown in LB broth. Because ivi proteins are expressed in response to specific cues during infection and might help pathogens adapt to and counter hostile in vivo environments, those identified in this study are potential targets for drug and vaccine development. Also, such proteins may be exploited as markers of O157 infection in stool specimens.
John
Manohar
M
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA. ikudva@partners.org
Kudva
Indira T
IT
Griffin
Robert W
RW
Dodson
Allen W
AW
McManus
Bethany
B
Krastins
Bryan
B
Sarracino
David
D
Progulske-Fox
Ann
A
Hillman
Jeffrey D
JD
Handfield
Martin
M
Tarr
Phillip I
PI
Calderwood
Stephen B
SB
D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism*; D003470:Culture Media; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015698:Genomic Library; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D020543:Proteome
D000942:Antigens, Bacterial; D003470:Culture Media; D029968:Escherichia coli Proteins; D007106:Immune Sera; D020543:Proteome