RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus.
The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.
Davis
Erica
E
Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.
Caiment
Florian
F
Tordoir
Xavier
X
Cavaillé
Jérôme
J
Ferguson-Smith
Anne
A
Cockett
Noelle
N
Georges
Michel
M
Charlier
Carole
C
D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D040461:Gene Components; D018392:Genomic Imprinting / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D040582:Inheritance Patterns / Q000235:genetics; D008322:Mammals / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism*; D016415:Sequence Alignment
D006023:Glycoproteins; D035683:MicroRNAs; D011506:Proteins; D012333:RNA, Messenger