Plasmid vectors for protein production, gene expression and molecular manipulations in Aspergillus niger.
We constructed three sets of plasmids for use in Aspergillus niger. These plasmids were assembled using various combinations of a series of modular DNA cassettes that included a selectable marker, pyrG, derived from Aspergillus nidulans; two promoter regions for directing protein expression; a cassette derived from the AMA1 replicator sequence to support autonomous replication; and a reporter gene based on the A. niger lacA gene. One set included integrating and autonomously replicating plasmids for the expression of homologous and heterologous proteins. The second was a set of autonomously replicating plasmids, with a secreted beta-galactosidase encoding reporter gene, for studying gene regulation events. The third set included pyrG-derived gene-blaster cassettes suitable for genome manipulation by targeted gene replacement.
Storms
Reginald
R
Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Que., Canada H4B 1R6. storms@vax2.concordia.ca
Zheng
Yun
Y
Li
Hongshan
H
Sillaots
Susan
S
Martinez-Perez
Amalia
A
Tsang
Adrian
A
D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000235:genetics*; D004261:DNA Replication; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017930:Genes, Reporter; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D004271:DNA, Fungal; D011506:Proteins; D001616:beta-Galactosidase