title: Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1.
abstract: The role of a macrophage galactose-type calcium-type lectin-1 (MGL1) in antigen-independent granulation tissue formation was investigated. Granulation tissue was induced by injection of carrageenan in an air pouch and distribution of macrophages expressing MGL1/2 was histologically examined. MGL1/2-positive cells were not observed in the granulation tissue induced by carrageenan though these cells were present in dermis. This was distinct from the fact that MGL1/2-positive cells were abundant in granulation tissue induced by antigenic stimulation. CD11b-positive cells were in dermis and carrageenan-induced granulation tissue. Because antigen-induced granulation tissue formation was previously shown to decrease in MGL1-deficient mice or after treatment with anti-MGL1 antibody, we investigated the effects of MGL1-deficient status on carrageenan-induced granulation tissue formation. The thickness of granulation tissue was almost identical between wild-type and MGL1-deficient mice. It is highly likely that MGL1-positive cells are not involved in tissue remodeling when inflammation is driven by nonspecific stimuli.
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Tokyo 113-0033, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Sato
authors.forename: Noriko
authors.initials: N
authors.lastname: Komatsu
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Higashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Imai
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
mesh_terms: D000818:Animals; D001212:Asialoglycoproteins; D002351:Carrageenan / Q000494:pharmacology; D006097:Granulation Tissue / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012867:Skin / Q000276:immunology*; D014945:Wound Healing / Q000276:immunology
chemical_list: D001212:Asialoglycoproteins; C064720:Clec10a protein, mouse; D037181:Lectins, C-Type; D008565:Membrane Proteins; D002351:Carrageenan