title: Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium.
abstract: Fully deuterated components from autotrophic cell lysate are useful materials for labeling of heterotrophs with deuterium. To facilitate the faster production of deuterated algal lysate, we selected a mutant Chlorella strain that grows faster in heavy water than the wild type. The mutant DR-17 was found to have a higher level of Hsp60 and an elevated level of protein synthesis. We previously isolated a deuterium-resistant yeast cell line that was also found to express elevated level of Hsp70 (K. Unno, T. Kishido, M. Morioka, S. Okada, and N. Oku, Biol. Pharm. Bull. 26:799-802, 2003). This suggests that the overexpression of heat shock proteins is required to compensate for the deuterium isotope effect.
authors.affiliation: Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. unno@u-shizuoka-ken.ac.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Unno
authors.forename: Naoko
authors.initials: N
authors.lastname: Hagima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kishido
authors.forename: Shoji
authors.initials: S
authors.lastname: Okada
authors.forename: Naoto
authors.initials: N
authors.lastname: Oku
mesh_terms: D002460:Cell Line; D018834:Chaperonin 60 / Q000096:biosynthesis*; D002708:Chlorella / Q000378:metabolism*; D003470:Culture Media; D041961:Deuterium Exchange Measurement; D017666:Deuterium Oxide / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D003470:Culture Media; D017666:Deuterium Oxide