title: Expression of gibberellin 3 beta-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry.
abstract: Expressions of the gibberellin biosynthesis gene were investigated in a normal upright type and a gravi-response mutant, a weeping type of Japanese flowering cherry (Prunus spachiana), that is unable to support its own weight and elongates downward. A segment of the gibberellin 3 beta-hydroxylase cDNA of Prunus spachiana (Ps3ox), which is responsible for active gibberellin synthesis, was amplified by using real-time RT-PCR. The content of Ps3ox mRNA in the weeping type was much greater than that in the upright type, while the endogenous gibberellin level was much higher in the elongating zone of the weeping type. These results suggest that the amount and distribution of synthesized gibberellin regulate secondary xylem formation, and the unbalanced distribution of gibberellin affects the gravi-response of the Prunus tree.
authors.affiliation: Graduate School of Science, Japan Women's University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Sugano
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nyunoya
authors.forename: Teruko
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000032:analysis / Q000378:metabolism; D018522:Gravitropism / Q000502:physiology*; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000378:metabolism*; D027861:Prunus / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D005875:Gibberellins; D012333:RNA, Messenger; C422660:gibberellin A1; D006899:Mixed Function Oxygenases; C108186:gibberellin 3beta-hydroxylase