title: Complete nucleotide sequences of two RNA segments of human picobirnavirus.
abstract: Picobirnaviruses are unclassified, non-enveloped, spherical, small viruses with a genome comprising two double-stranded RNA segments. Only incomplete sequence data on picobirnaviruses are available so far. By cloning involving single primer amplification, full-length cDNAs were prepared corresponding to RNA segments 1 and 2 of a picobirnavirus (strain Hy005102) isolated from a stool specimen from an infant with acute non-bacterial gastroenteritis in Thailand, and the complete nucleotide sequences were determined. RNA segments 1 and 2 are 2,525 and 1,745 base pairs in length, respectively. RNA segment 1 encodes two open reading frames (ORFs) of 224 and 552 amino acids, and RNA segment 2 codes for a single ORF of 534 amino acids. On comparison with a part of the nucleotide sequences of the RNA segment, 2 of the other published picobirnavirus strains, the Thai strain was found to be related most closely to one of the US strains.
authors.affiliation: Department of Virology and Parasitology, Fujita Health University, School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Wakuda
authors.forename: Yaowapa
authors.initials: Y
authors.lastname: Pongsuwanna
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D001483:Base Sequence; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D018708:Picobirnavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012327:RNA Virus Infections / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand
chemical_list: D012367:RNA, Viral