Effect of IGL-1, a new preservation solution, on kidney grafts (a pre-clinical study).
Ischemia-reperfusion injury conditions short-term and long-term graft function. The effects of the inversion of K+ and Na+ concentrations and substitution with polyethylene glycol for hydroxyethyl starch in University of Wisconsin (K-UW) solution were evaluated in isolated perfused rat kidneys and in autotransplanted pig kidneys. In the rat model kidneys were cold-stored for 24 h in K-UW or Na-UW or Na-PEG UW solutions (IGL-1 solution). Fractional sodium reabsorption and glomerular filtration rate was better in kidneys preserved in Na-UW and IGL-1 solution than those preserved in K-UW solution. In the pig model the left kidney was harvested and preserved in K-UW or IGL-1 solution for 24 h and then transplanted. In the autotransplanted pig model, kidneys preserved in IGL-1 solution showed a better function and a significant reduction of MHC class II expression, cellular apoptosis and interstitial fibrosis. In conclusion, kidneys preserved in IGL-1 solution tolerated ischemia/reperfusion injury better than those preserved in K-UW solution.
Badet
Lionel
L
Service d'Urologie et Transplantation, Hopital Edouard Herriot University Claude Bernard Lyon I, 5 Place d'Arsonval, 69437 Lyon Cedex 03, France. lionel.badet@chu-lyon.fr
Ben Abdennebi
Hassen
H
Petruzzo
Palmina
P
McGregor
Brigitte
B
Espa
Michele
M
Hadj-Aissa
Aoumeur
A
Ramella-Virieux
Silvina
S
Steghens
Jean Paul
JP
Portoghese
Francesca
F
Martin
Xavier
X
D000818:Animals; D004353:Drug Evaluation, Preclinical; D066298:In Vitro Techniques; D016030:Kidney Transplantation; D008297:Male; D023421:Models, Animal; D019188:Organ Preservation Solutions / Q000737:chemistry / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D014182:Transplantation, Autologous
C504757:IGL-1 solution; D019188:Organ Preservation Solutions