title: Polymorphisms of genes involved in homocysteine metabolism in preeclampsia and in uncomplicated pregnancies.
abstract: OBJECTIVE
  To evaluate the possible relationship between preeclampsia and polymorphisms in the main genes involved in folate-homocysteine metabolism.
  
  
  METHODS
  Case-control study: 43 patients with preeclampsia and 122 controls without pregnancy complications. Laboratory studies: tHcy and other amino acids, folate and vitamin B(12) and polymorphisms: 677C > T and 1298A > C (MTHFR); 699C > T, 844ins68 and 1080C > T (CBS); 2756A > G (MTR); and 66G > A, IVS1+766G > A and IVS1+754A > C (MTRR).
  
  
  RESULTS
  Plasma tHcy and folate values were significantly higher (P = 0.004 and P = 0.019), while Met/tHcy ratios were lower (P < 0.001) in the patients compared with controls. No association was observed between polymorphisms tested and preeclampsia. In the control group, four such associations were found: the 1298A > C polymorphism (MTHFR) with the ratio Met/tHcy (P = 0.014); the 699C > T polymorphism (CBS) with the ratio tHcy/SigmaAA (P = 0.013); the 2756A > G polymorphism (MTR) with tHcy (P = 0.034); and the IVS1+766G > A polymorphism (MTRR) with hyperhomocysteinemia (P = 0.012).
  
  
  CONCLUSIONS
  An association between the polymorphisms analysed and preeclampsia could not be demonstrated.
authors.affiliation: Department of Genetics of the University of Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Also-Rallo
authors.forename: Eva
authors.initials: E
authors.lastname: Lopez-Quesada
authors.forename: Roser
authors.initials: R
authors.lastname: Urreizti
authors.forename: M Antònia
authors.initials: MA
authors.lastname: Vilaseca
authors.forename: Josep M
authors.initials: JM
authors.lastname: Lailla
authors.forename: Susana
authors.initials: S
authors.lastname: Balcells
authors.forename: Daniel
authors.initials: D
authors.lastname: Grinberg
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics; D000328:Adult; D016022:Case-Control Studies; D003541:Cystathionine beta-Synthase / Q000235:genetics; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics; D005492:Folic Acid / Q000097:blood; D006710:Homocysteine / Q000097:blood / Q000378:metabolism*; D006801:Humans; D020138:Hyperhomocysteinemia / Q000235:genetics; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D014805:Vitamin B 12 / Q000097:blood
chemical_list: D006710:Homocysteine; D005492:Folic Acid; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D003541:Cystathionine beta-Synthase; D014805:Vitamin B 12