title: Identification of dynein light chain 2 as an interaction partner of p21-activated kinase 1.
abstract: p21-Activated kinase 1 (PAK1), a member of the evolutionarily conserved PAK family of serine/threonine kinases, is essential for a variety of cellular functions. Our previous studies showed that PAK1 participated in the apoptotic pathway mediated by p110C. To further investigate its functions, we used the yeast two-hybrid system to screen a human fetal brain cDNA library and identified dynein light chain 2 (DLC2)/myosin light chain (MLC) as an interacting partner of PAK1. The association of PAK1 with DLC2 was further confirmed by in vitro binding assay. With the stimulation of EGF, PAK1 interacted with HA-DLC2 in vivo and relocalized in cytoplasm near the perinuclear location in confocal microscope analysis. The deletion analysis showed that the interaction of DLC2 with PAK1 occurred within the residues 210-332 of PAK1. For that studies showed that DLC2 was a subunit of myosin complex, so it is possible that PAK1 binds to DLC2 and transports by myosin complex.
authors.affiliation: State Key Laboratory of Genetic Engineering and Gene Research Center, Shanghai Medical College of Fudan University, Box 103, Shanghai 200032, People's Republic of China.
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
authors.forename: Hanzhou
authors.initials: H
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Hu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D001665:Binding Sites; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology; D056445:Cytoplasmic Dyneins; D004398:Dyneins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D054462:p21-Activated Kinases
chemical_list: C515903:PAK1 protein, human; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases; D056445:Cytoplasmic Dyneins; C501531:DYNLL2 protein, human; D004398:Dyneins