title: [The relationship between the expressions of interleukin-8 and cyclooxygenase-2 and the type of anastomosis in the remnant stomach].
abstract: OBJECTIVE
  To study the expressions and the significance of interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2) in the remnant stomach.
  
  
  METHODS
  Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Two biopsy specimens were obtained from the stoma and the upper corpus gastric mucosa in the remnant stomach. mRNA was extracted from biopsy specimens to measure the IL-8 and COX-2 gene mRNA levels by real-time PCR method.
  
  
  RESULTS
  IL-8 and COX-2 levels were higher in stoma than in corpus, IL-8 levels in BI anastomosis were significantly higher in stoma than in corpus (P< 0.05). In Hp-negative patients, IL-8 and COX-2 levels in stoma were significantly higher in BII anastomosis than in BI anastomosis (P < 0.05). In Hp-positive patients, IL-8 and COX-2 levels in stoma showed no significant differences between BII anastomosis and BI anastomosis. In corpus, IL-8 and COX-2 levels in Hp-positive patients were significantly higher than those in Hp-negative patients, (P < 0.05), including in BI anastomosis and in BII anastomosis.
  
  
  CONCLUSIONS
  The risk of the secondary stomach carcinogenesis in stoma after distal gastrectomy is higher than that in corpus; The types of anastomosis may influence the risk for the secondary stomach carcinogenesis in the remnant stomach mucosa.
authors.affiliation: Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Cheng-Zhong
authors.initials: CZ
authors.lastname: Xing
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Guo
authors.forename: Hui-Mian
authors.initials: HM
authors.lastname: Xu
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D018530:Gastric Stump / Q000601:surgery*; D005763:Gastroenterostomy / Q000009:adverse effects / Q000379:methods*; D016481:Helicobacter Infections; D016480:Helicobacter pylori; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000209:etiology*
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases