[Random amplified polymorphic DNA analysis of tomato from seeds carried in Russian Mir space station].
OBJECTIVE
To identify the variation of hereditary substance of tomato offspring from seeds under long-duration spaceflight condition.


METHODS
The tomato seeds carried in Russian MIR space station for six years, and the ground-based control were planted on the ground. The leaves of plants were used to do random amplified polymorphic DNA (RAPD) analysis.


RESULTS
Forty random primers were used in this study, among which 31 primers generated the same DNA band type, and 9 primers generated different DNA band types. Forty primers amplified 269 DNA bands, among which 29 DNA bands were polymorphic ones with a percentage of polymorphism of 10.8%. Compared with the control, plants from seeds carried in space station generated different band types. Band types were different among five plants from seeds carried in space station. The number of polymorphic bands generated by plant No. 5 compared with the control was the most, and that by plant No. 2 was the least.


CONCLUSIONS
Long-duration spaceflight can cause variation of hereditary substance DNA of tomato.
Lu
Jin-ying
JY
Liu
Min
M
Xue
Huai
H
Pan
Yi
Y
Zhang
Chun-hua
CH
Nechitailo
Galina S
GS
D018744:DNA, Plant / Q000032:analysis*; D014644:Genetic Variation; D018551:Solanum lycopersicum / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000235:genetics*; D013026:Space Flight; D014893:Weightlessness
D018744:DNA, Plant