title: An efficient preparation of polyanionic affinity agent and its evaluation for the measurement of glycated hemoglobin.
abstract: An efficient method was developed for the preparation of polyanionic affinity agent (3), a key component in the measurement of glycated hemoglobin (GHb). Glycated hemoglobin is an important clinical marker for diagnosis of patients with diabetes and useful to monitor the management of disease. The affinity agent (3) was prepared based on coupling reaction between poly(acrylic acid) (1) and 3-aminophenylboronic acid (2) in water. The critical features of this polymeric affinity agent (3), such as size, boronic acid incorporation ratio and concentration, on the measurement of glycated hemoglobin were evaluated. It was found that the agent (3) prepared using poly(acrylic acid) (1) with 225 kDa molecular weight gave optimal GHb measurement. The performance test results demonstrated that the boronic acid incorporation ratio and concentration of affinity agent (3) play a critical role in the assay and determines the precision of glycated hemoglobin measurement.
authors.affiliation: Core R&D Chemistry Group, (Dept: 09MD, Bldg: AP20), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064-6016, USA. rajarathnam.reddy@abbott.com
authors.forename: Rajarathnam E
authors.initials: RE
authors.lastname: Reddy
authors.forename: You
authors.initials: Y
authors.lastname: Pan
authors.forename: Donald D
authors.initials: DD
authors.lastname: Johnson
authors.forename: Yon-Yih
authors.initials: YY
authors.lastname: Chen
authors.forename: Saul A
authors.initials: SA
authors.lastname: Datwyler
authors.forename: Michelle S
authors.initials: MS
authors.lastname: Hauptman
authors.forename: John K
authors.initials: JK
authors.lastname: Thottathil
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001897:Boronic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D002846:Chromatography, Affinity; D005069:Evaluation Studies as Topic; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism*; D006801:Humans
chemical_list: D000180:Acrylic Resins; D001897:Boronic Acids; D006442:Glycated Hemoglobin; C028592:3-aminobenzeneboronic acid; C006903:carbopol 940