Relapsing fever spirochetes contain chromosomal genes with unique direct tandemly repeated sequences.
Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated amino acid sequences that were absent in the Lyme disease spirochete Borrelia burgdorferi. The predicted amino acid sequences of these genes (BH0209, BH0512, and BH0553) have hydrophobic N termini, indicating that these proteins may be secreted. B. hermsii transcribed the three ORFs in vitro, and the BH0512- and BH0553-encoded proteins (PBH-512 and PBH-553) were produced in vitro and in experimentally infected mice. PBH-512 and PBH-553 were on the spirochete's outer surface, and antiserum to these proteins reduced the adherence of B. hermsii to red blood cells. PCR analyses of 28 isolates of B. hermsii and 8 isolates of B. turicatae demonstrated polymorphism in each gene correlated with the number of repeats. Serum samples from relapsing fever patients reacted with recombinant PBH-512 and PBH-553, suggesting that these proteins are produced during human infection. These polymorphic proteins may be involved in the pathogenicity of these relapsing fever spirochetes and provide a mechanism for antigenic heterogeneity within their populations.
Guyard
Cyril
C
Rocky Mountain Laboratories, 903 S. Fourth St., Hamilton, MT 59840, USA. cguyard@niaid.nih.gov
Chester
Earl M
EM
Raffel
Sandra J
SJ
Schrumpf
Merry E
ME
Policastro
Paul F
PF
Porcella
Stephen F
SF
Leong
John M
JM
Schwan
Tom G
TG
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001898:Borrelia / Q000235:genetics / Q000276:immunology; D002876:Chromosomes, Bacterial / Q000235:genetics; D004285:Dogs; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012061:Relapsing Fever / Q000276:immunology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*
D001426:Bacterial Proteins; C500903:PBH-512 protein, Borrelia hermsii; C500904:PBH-553 protein, Borrelia hermsii; D011994:Recombinant Proteins