Design of peptides for thin films, coatings and microcapsules for applications in biotechnology.
A highly-interdisciplinary approach has been developed for minimizing the immunogenicity of films, coatings, microcapsules and other nano-structured materials fabricated from designed polypeptide chains. It is to base the amino-acid sequences on solvent-exposed regions in the folded states of proteins from the same organism. Each such region that meets defined criteria with respect to charge is called a sequence motif. The approach becomes more specifically tailored for intravenous applications by requiring an employed sequence motif to correspond to a known blood protein. An algorithm has been developed to identify sequence motifs in protein-encoding regions of a genome. This work is focused on sequence motifs of charge per unit length >0.5 at neutral pH. It has been found that the number of unique sequence motifs meeting this criterion in available human genome data is maximal for motifs of approx. 7 residues in length. We have designed polypeptides on the basis of computational analysis and shown that they can be used to fabricate nano-structured thin films by electrostatic layer-by-layer assembly (ELBL). The results of this work are discussed with a view to possible applications in biotechnology, notably development of biocompatible coatings and microcapsules.
Zheng
Bin
B
Computational Analysis and Modeling, College of Engineering and Science, Louisiana Tech University, Ruston, LA 71272, USA.
Haynie
Donald T
DT
Zhong
Hua
H
Sabnis
Kaustubh
K
Surpuriya
Vinay
V
Pargaonkar
Nikhil
N
Sharma
Gyanesh
G
Vistakula
Kranthi
K
D020816:Amino Acid Motifs; D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002214:Capsules / Q000737:chemistry*; D015195:Drug Design; D016678:Genome; D015894:Genome, Human; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D015202:Protein Engineering; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry; D012997:Solvents; D055672:Static Electricity
D001672:Biocompatible Materials; D002214:Capsules; D010455:Peptides; D011108:Polymers; D011506:Proteins; D012997:Solvents