Selective influence of dentin thickness upon cytotoxicity of dentin contacting materials.
The effect of three dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device. The test materials (Syntac Classic, Prompt L-Pop, Vitrebond) were applied on dentin disks of different thicknesses ranging from 100 to 500 microm. After 24 h of exposure with and without perfusion of the test chamber, cell survival was evaluated using the MTT assay and related to a nontoxic control material. Syntac Classic decreased cell activity significantly (p </= 0.0003), independently of the dentin thickness. For Prompt L-Pop and Vitrebond a significant influence of dentin thickness was found on the cell reaction. After exposure of the control material, photometric readings showed no dependency of the cellular reaction on dentin thickness (p > 0.05). It could be demonstrated that dentin acts as a barrier, decreasing the elicited cytotoxicity with increasing thickness. This effect is material related, showing little influence for the nontoxic or the glutaraldehyde containing material.
Galler
Kerstin
K
Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany.
Hiller
Karl-Anton
KA
Ettl
Tobias
T
Schmalz
Gottfried
G
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003804:Dentin / Q000033:anatomy & histology* / Q000502:physiology; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D015194:Diffusion Chambers, Culture; D005899:Glass Ionomer Cements / Q000633:toxicity; D008422:Materials Testing; D019279:Resin Cements / Q000633:toxicity
D017220:Dentin-Bonding Agents; D005899:Glass Ionomer Cements; C408584:Prompt L-Pop; D019279:Resin Cements; C078484:Syntac dentine adhesive; C067790:Vitrabond