Use of polymerase chain reaction analysis of urinary DNA to detect bladder carcinoma.
A cohort of 113 patients underwent prospective evaluation with a panel of seven microsatellites, on chromosomes 9, 13 and 17. Thirty-seven patients had histologically confirmed bladder tumors, 53 patients had a history of previous transitional cell carcinoma of the bladder (TCC) but normal cystoscopies (control Group 1), and 23 patients had no previous history of TCC and normal cystoscopies (control Group 2). Urinary DNA was considered to show a deletion if an allele was reduced by more than 50%, and this was considered diagnostic of bladder carcinoma. The sensitivity of the method was 50%, positive predictive value was 80%, and specificity was 93%. Reducing the threshold for defining allelic loss increases sensitivity, but reduces specificity. The concentration of urinary DNA in the sample did not influence detection rate. The grade and stage of the bladder tumor did not influence the likelihood of detection. This method detects bladder carcinoma with high specificity, and increasing the number of microsatellites used should increase sensitivity.
Little
Brian
B
Craigavon Area Hospital, Craigavon, Northern Ireland. brianlittle@doctors.org.uk
Hughes
Anne
A
Young
Michael R A
MR
O'Brien
Aiden
A
D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D002882:Chromosomes, Human, Pair 13; D002886:Chromosomes, Human, Pair 17; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics*
D004273:DNA, Neoplasm