title: Visualizing adhesion-induced agglutination of Escherichia coli with mannosylated nanoparticles.
abstract: Polymeric nanoparticles covalently functionalized with derivatized D-mannose molecules were synthesized and characterized. These nanoparticles have an average size of approximately 160 nm in diameter, thus bearing a large number of surface-tethered mannose moieties for multivalent interactions with adhesins on bacterial cells. Specifically, the mannosylated nanoparticles bind strongly with Escherichia coli, allowing the convenient visualization of adhesion interactions under a conventional electron microscope. Since a single nanoparticle is capable of binding more than one cell, the adhesion interactions result in significant nanoparticle-mediated cell agglutination according to electron microscopy imaging. Potential applications of the mannosylated nanoparticles in the inhibition of enteropathogenic infections are discussed.
authors.affiliation: Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.
authors.forename: Liangwei
authors.initials: L
authors.lastname: Qu
authors.forename: Pengju G
authors.initials: PG
authors.lastname: Luo
authors.forename: Shelby
authors.initials: S
authors.lastname: Taylor
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Weijie
authors.initials: W
authors.lastname: Huang
authors.forename: Nkechinyere
authors.initials: N
authors.lastname: Anyadike
authors.forename: Tzuen-Rong J
authors.initials: TR
authors.lastname: Tzeng
authors.forename: Fred
authors.initials: F
authors.lastname: Stutzenberger
authors.forename: Robert A
authors.initials: RA
authors.lastname: Latour
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Sun
mesh_terms: D018830:Adhesins, Escherichia coli / Q000378:metabolism; D000371:Agglutination / Q000187:drug effects / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008358:Mannose / Q000737:chemistry* / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods
chemical_list: D018830:Adhesins, Escherichia coli; D008358:Mannose