title: [Preliminary research on bacterial diversity of Parece Vela Basin, Pacific Ocean by culture-independent method].
abstract: The environmental DNA was directly extracted from the sediment in Parece Vela Basin, Pacific Ocean, at a depth of 5010 m. Bacterial 16S rRNA gene library of 32 clones was generated using bacterial universal primers and 16S rDNA sequences were analyzed phylogenetically. 17 phylotypes were obtained. The library was dominated by gamma-Proteobacteria, alpha-Proteobacteria and marine uncultured bacteria. Sixty-two percent of the cloned sequences was highly related to the known bacteria in the genus Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, Pseudoalteromonas (> 96% sequence similarity), while some of the cloned sequences showed less affiliation with known taxa (< 94% sequence similarity) and may represent novel taxa.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. huaxie399@hotmail.com
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.forename: Yan-fen
authors.initials: YF
authors.lastname: Xue
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhao
authors.forename: Tie-gang
authors.initials: TG
authors.lastname: Li
authors.forename: Yan-he
authors.initials: YH
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S