Lipid hydroperoxidase activity of myoglobin and phenolic antioxidants in simulated gastric fluid.
Our recent study demonstrated the potential of gastric fluid at pH 3.0 to accelerate lipid peroxidation and cooxidation of dietary constituents in the stomach medium. Metmyoglobin is known to catalyze the breakdown of lipid hydroperoxides to free radicals, a reaction that could enhance the propagation step and general lipid peroxidation. During this reaction, a part of the free radicals is autoreduced by metmyoglobin. At pH 3.0, metmyoglobin at low concentration was almost 7 x 10(4) times as effective as at pH 7.0 in enhancing the rate of lipid peroxidation. Our study demonstrated that metmyoglobin, at a low concentration (approximately 1:30), as compared with that of the hydroperoxides in the lipid system, worked prooxidatively increasing the amounts of linoleate hydroperoxides. However, at a high concentration (approximately 1:3), metmyoglobin acted antioxidatively and decomposed hydroperoxides, whose concentrations then remained at zero for a long time. Catechin, a known polyphenol, supports the inversion of metmyoglobin catalysis, from prooxidation to antioxidation. The antioxidative activity of the couple metmyoglobin-catechin was better at pH 3.0 than at pH 7.0, indicating that this reaction is more dependent on metmyoglobin than on catechin. During this reaction, catechin or quercetin not only donates reducing equivalents to prevent lipid peroxidation but also prevents the destruction and polymerization of metmyoglobin. The results of this research highlighted the important and possible reactions of heme proteins and polyphenols as couple antioxidants, working as hydroperoxidases or as pseudo-peroxidases. We hypothesize that the occurrence of these reactions in the stomach could have an important impact on our health and might help to better explain the health benefits of including foods rich in polyphenol antioxidants in the meal, especially when consuming red meat.
Lapidot
Tair
T
Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel.
Granit
Rina
R
Kanner
Joseph
J
D000975:Antioxidants / Q000378:metabolism*; D002392:Catechin / Q000008:administration & dosage / Q000378:metabolism; D005750:Gastric Juice / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation; D008786:Metmyoglobin / Q000008:administration & dosage / Q000737:chemistry; D008954:Models, Biological; D009211:Myoglobin / Q000008:administration & dosage / Q000378:metabolism*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011108:Polymers; D013024:Soybean Oil / Q000737:chemistry
D000975:Antioxidants; D009211:Myoglobin; D010636:Phenols; D011108:Polymers; D008786:Metmyoglobin; D013024:Soybean Oil; D002392:Catechin; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D010544:Peroxidases