title: Antimutagenic constituents from the thorns of Gleditsia sinensis.
abstract: Antimutagenic activity-guided fractionation of an extract prepared from the thorns of Gleditsia sinensis LAM. led to the isolation of one triterpenoid and four steroids, which were identified as D:C-friedours-7-en-3-one (1), stigmast-4-ene-3,6-dione (2), stigmastane-3,6-dione (3), stigmasterol (4), and beta-sitosterol (5). Triterpenoid 1 was found for the first time in a natural source and the steroids 2-5 were first isolated from this plant. Stigmasterol was the most active antimutagen, showing 51.2% and 64.2% reduction of the induction factor against the mutagens MNNG and NQO, respectively, in the SOS chromotest. Some NMR data of the steroids 2 and 3 obtained have to be revised.
authors.affiliation: Department of Biotechnology, Dongguk University, Gyeongju 780-714, Korea.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Milos
authors.initials: M
authors.lastname: Budesinsky
authors.forename: Alexander
authors.initials: A
authors.lastname: Kasal
authors.forename: Yeong-Hwan
authors.initials: YH
authors.lastname: Han
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Koo
authors.forename: Seung-Il
authors.initials: SI
authors.lastname: Lee
authors.forename: Dong-Ung
authors.initials: DU
authors.lastname: Lee
mesh_terms: D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031501:Gleditsia; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018514:Plant Structures; D013234:Stem Cells / Q000187:drug effects
chemical_list: D016587:Antimutagenic Agents; D010936:Plant Extracts