title: Efficient callus induction and plant regeneration from anther of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem).
abstract: Callus culture has, to date, been reported only in a few species of Narcissus. We used anthers of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem) as explants for callus induction and plant regeneration. A high percentage of anthers at the early- to mid-uninucleate microspore stage were responsive on the basal MS medium supplemented with 0.5-1 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.5-2 mg l(-1) 6-benzyladenine under dark conditions. Calli were initiated from anther connective tissue or anther wall tissue, and no division of microspores occurred during callus formation, as determined by histological observation. Using 20 random amplified polymorphic DNA primers, we verified the genetic integrity of the anther-derived plants of Chinese narcissus with respect to the donor plants. These results suggest that anther culture in vitro can provide an efficient new micropropagation technique for Chinese narcissus as well as a new strategy for in vitro mass propagation of other daffodils.
authors.affiliation: Department of Biology, School of Life Science, Xiamen University, Xiamen 361005, China. linjiaochen@163.com
authors.forename: LinJiao
authors.initials: L
authors.lastname: Chen
authors.forename: XueYi
authors.initials: X
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000383:Agriculture / Q000379:methods; D001593:Benzyl Compounds; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D018745:Genome, Plant; D005838:Genotype; D007701:Kinetin / Q000494:pharmacology; D031427:Narcissus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D011687:Purines; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D013170:Spores / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
chemical_list: D001593:Benzyl Compounds; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C480551:benzylaminopurine; D007701:Kinetin