title: Association mechanism between a series of rodenticide and humic acid: a frontal analysis to support the biological data.
abstract: The binding constants (K) of a series of anticoagulant rodenticides with the main soil organic component, humic acid (HA), were determined using frontal analysis approach. The order of the binding constants was identical as the one obtained in a previous paper [J. Chromatogr. B 813 (2004) 295], i.e. bromadiolone>brodifacoum>difenacoum>chlorophacinone>diphacinone, confirming the power of this frontal analysis approach for the determination of binding constants. Moreover, and for the first time, the concentration of unbound rodenticide to HAs could be determined. Thanks this approach, we could clearly demonstrate that HA acid protected the human hepatoma cell line HepG2 against the cytotoxicity of all the rodenticides tested and that the toxicity of rodenticides was directly linked to the free rodenticide fraction in the medium (i.e. unbound rodenticide to HA).
authors.affiliation: Equipe Sciences Séparatives et Biopharmaceutiques (2SB/EA-3124), Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place St. Jacques, 25030 Besançon Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: André
authors.forename: Catherine
authors.initials: C
authors.lastname: Guyon
authors.forename: Mireille
authors.initials: M
authors.lastname: Thomassin
authors.forename: Alexandre
authors.initials: A
authors.lastname: Barbier
authors.forename: Lysiane
authors.initials: L
authors.lastname: Richert
authors.forename: Yves-Claude
authors.initials: YC
authors.lastname: Guillaume
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006812:Humic Substances; D012378:Rodenticides / Q000737:chemistry* / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D000925:Anticoagulants; D006812:Humic Substances; D012378:Rodenticides