Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium.
Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.
MacFadyen
John R
JR
The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, UK.
Haworth
Oliver
O
Roberston
David
D
Hardie
Deborah
D
Webster
Marie-Therese
MT
Morris
Howard R
HR
Panico
Maria
M
Sutton-Smith
Mark
M
Dell
Anne
A
van der Geer
Peter
P
Wienke
Dirk
D
Buckley
Christopher D
CD
Isacke
Clare M
CM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers / Q000378:metabolism*; D019556:COS Cells; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004730:Endothelium, Vascular / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D020286:Pericytes / Q000378:metabolism; D011233:Precipitin Tests; D017154:Stromal Cells / Q000378:metabolism*; D013388:Succinimides; D014471:Umbilical Veins / Q000166:cytology
C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; C078203:CD248 protein, human; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D013388:Succinimides