The inflammatory response to vaccination is altered in the elderly.
To further explore whether immune function and acute phase response are altered during ageing, the response to a mild inflammatory stress (DT-Polio-Typhim vaccination) was studied in elderly and young subjects. Cytokine production (IFN-gamma, TNF-alpha, IL-6, IL-10) by whole blood cultures, circulating cytokines and acute phase proteins were analysed before and 2 days after vaccination. Prior to vaccination, only IFN-gamma production was lower in the elderly than in the young subjects due to a lower mononuclear cell number. In the same time, although in the normal range, several acute phase proteins were greater in elderly than in young subjects, suggesting a low-grade inflammatory state in the elderly. After vaccination, IFN-gamma production remained lower in the elderly than in the young, supporting an altered cell-mediated immunity with advancing age. TNF-alpha production was unaffected by either ageing or vaccination. IL-6 production was stimulated by vaccination in young subjects but not significantly in the elderly. IL-10 production was inhibited by vaccination in the elderly but not in the young. Acute phase proteins were less increased in elderly than in young subjects. Taken together, these results support a general lack of inflammatory response in the elderly exposed to an immune challenge and suggest that immune deficiency may concern both Th1 and Th2 responses. However, the interpretation must respect the limitation of small subjects number.
El Yousfi
Mimoun
M
Unité de Nutrition & Métabolisme Protéique, INRA de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France.
Mercier
Sabine
S
Breuillé
Denis
D
Denis
Philippe
P
Papet
Isabelle
I
Mirand
Philippe Patureau
PP
Obled
Christiane
C
D000209:Acute-Phase Proteins / Q000378:metabolism; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D016207:Cytokines / Q000096:biosynthesis; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014436:Typhoid-Paratyphoid Vaccines; D014611:Vaccination
D000209:Acute-Phase Proteins; D016207:Cytokines; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014436:Typhoid-Paratyphoid Vaccines; D016753:Interleukin-10; D007371:Interferon-gamma