title: Molecular characterization of a new serovar of Salmonella bongori 13,22:z39:- isolated from a lizard.
abstract: Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z39:-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to the S. bongori species, several genes were amplified and/or sequenced. The iroB gene has been reported to be present only in S. enterica, while the invA gene has been described as being a helpful tool in distinguishing Salmonella from other bacterial species. Both genes were amplified and, as expected, only invA could be amplified. The fliC gene, encoding the phase 1 flagellin fljB gene, encoding phase 2 flagellin, and the gapA gene, which is believed to present polymorphic alleles among different subspecies, were amplified and sequenced. The sequence obtained from fliC(z39) matched with the sequences fliC(z39) obtained from other serovars. The sequence obtained from gapA clustered into the S. bongori group when it was compared to others previously described. We conclude that these three isolates are members of the S. bongori species representing a new serovar that will be described in the next supplement to the Kauffmann-White scheme.
authors.affiliation: Laboratorio Nacional de Referencia de Salmonella y Shigella, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. sherrera@isciii.es
authors.forename: Silvia
authors.initials: S
authors.lastname: Herrera-León
authors.forename: Montserrat
authors.initials: M
authors.lastname: Saco
authors.forename: Albert
authors.initials: A
authors.lastname: Martínez Silvestre
authors.forename: Luis
authors.initials: L
authors.lastname: Silveira
authors.forename: Aurora
authors.initials: A
authors.lastname: Echeita
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Usera
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D005408:Flagellin / Q000235:genetics; D008116:Lizards / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification / Q000235:genetics*; D013030:Spain
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C094427:FljB protein, Salmonella; C117938:GapA cytadhesin; D005408:Flagellin; C075518:invA protein, Bacteria