Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana.
Chromosomal beta-lactamase genes (bla(KLUY)) from six Kluyvera georgiana strains isolated in Guyana were cloned and expressed in Escherichia coli. KLUY-1 exhibited 100% amino acid identity with the extended-spectrum beta-lactamase CTX-M-14. We also show that a 2.7-kb Kluyvera chromosomal region exhibits 99% nucleotide identity to a portion of In60 that includes bla(CTX-M-9).
Olson
Adam B
AB
University of Manitoba, Winnipeg, Canada.
Silverman
M
M
Boyd
David A
DA
McGeer
Allison
A
Willey
Barbara M
BM
Pong-Porter
V
V
Daneman
N
N
Mulvey
Michael R
MR
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D006172:Guyana; D006801:Humans; D044086:Kluyvera / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D015246:Deoxyribonuclease EcoRI; C412613:CTX-M-9 protein, E coli; D001618:beta-Lactamases