Structure of the O-polysaccharide from the lipopolysaccharide of Providencia stuartii O43 containing an amide of D-galacturonic acid with L-serine.
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O43:H28 and studied by sugar and methylation analyses, Smith degradation and 1H and 13C NMR spectroscopy, including 2D ROESY, and H-detected 1H, 13C HSQC and HMBC experiments, as well as a NOESY experiment in a 9:1 H2O/D2O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear tetrasaccharide repeating units containing an amide of D-galacturonic acid with L-serine [D-GalA6(L-Ser)] and has the following structure:[3)-beta-D-GalpA6(L-Ser)-(1-->3)-beta-D-GlcpNAc-(1-->2)-alpha-D-Rhap4NAc-(1-->4)-beta-D-GlcpA-(1-->]n.
Ovchinnikova
Olga G
OG
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. olgao@hotmail.ru
Kocharova
Nina A
NA
Torzewska
Agnieszka
A
Blaszczyk
Aleksandra
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D000577:Amides / Q000737:chemistry; D002240:Carbohydrate Sequence; D006603:Hexuronic Acids / Q000737:chemistry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry* / Q000276:immunology; D012694:Serine / Q000737:chemistry
D000577:Amides; D006603:Hexuronic Acids; D019081:O Antigens; C504166:O-polysaccharide, Providencia stuartii O43; D011135:Polysaccharides, Bacterial; D012694:Serine; C007819:galacturonic acid