Comparative molecular analysis of Haemophilus influenzae isolates from young children with acute lower respiratory tract infections and meningitis in Hanoi, Vietnam.
Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no beta-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns alpha (22 isolates) and beta (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam.
Watanabe
Hiroshi
H
Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. h-wata@net.nagasaki-u.ac.jp
Kaji
Chiharu
C
Anh
Dang Duc
DD
Huong
Phan Le Thanh
Ple T
Anh
Nguyen Thi Hien
NT
Huong
Vu Thi
VT
Phuong
Hoang Vu Mai
HV
Thi
Ngo Thi
NT
Suu
Pham Thi
PT
Nguyet
Nguyen Thi Thu
NT
Rusizoka
Olivia Sebastian
OS
Watanabe
Kiwao
K
Nagatake
Tsuyoshi
T
Oishi
Kazunori
K
D000907:Antibodies, Bacterial / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006192:Haemophilus Infections / Q000175:diagnosis*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D016920:Meningitis, Bacterial / Q000175:diagnosis*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D015183:Restriction Mapping
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial