Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry.
A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM diethyl dithiocarbamate. Each extract was partially purified in C18 cartridge column for solid-phase extraction (SPE) and subjected to HPLC-ESI-MS/MS without converting the product. The detection limit was 3.8 fmol for IAA, and 0.4-2.9 fmol for the IAA amino acid conjugates. The method was applied to the analysis of IAA and its conjugates in rice seedlings, dehulled rice and calli, using 20-100 mg tissue samples.
Matsuda
Fumio
F
Plant Functions and Their Control, CREST, Japan Science and Technology Agency, Tokyo 103-0027, Japan.
Miyazawa
Haruna
H
Wakasa
Kyo
K
Miyagawa
Hisashi
H
D000596:Amino Acids / Q000032:analysis / Q000737:chemistry*; D002853:Chromatography, Liquid; D007210:Indoleacetic Acids / Q000032:analysis* / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D000596:Amino Acids; D007210:Indoleacetic Acids; C030737:indoleacetic acid