Identification of three novel HLA class I alleles: HLA-A*0261, HLA-B*1585 and HLA-B*1587.
Three novel human leucocyte antigen (HLA) class I alleles have been characterized by means of direct DNA sequencing analysis. HLA-A* 0261 showed sequence variation at conserved codon. It differs from HLA-A* 020601 by a single-nucleotide substitution at codon 57 (CCG-->GCG) resulting in an amino acid change from Pro to Ala. The sequences of HLA-B*1585 are similar to those of HLA-B*15010101, but differed five nucleotides on exon 3 resulting in three amino acid changes at residues 94 (Thr-->Ile), 95 (Leu-->Ile) and 103 (Val-->Leu). Likewise, HLA-B*1587 is identical to HLA-B*15010101 except at codons 80-83 (Asn-Leu-Arg-Gly-->Ile-Ala-Leu-Arg) which has been replaced by HLA-Bw4 motif. These alleles seemed to be generated by either a point mutation or a gene conversion-like event from alleles existing in the population with high frequencies.
Lee
K W
KW
Hallym Institution for Genome Application, Hallym University, Sacred Heart Hospital, #896 Pyungchon-Dong, Dongan-Ku, Anyang, Kyungki-Do 431-070, Korea. hlakw@hanmail.net
Cho
S
S
D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005785:Gene Conversion; D005787:Gene Frequency; D005805:Genes, MHC Class I; D015234:HLA-A Antigens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D059910:HLA-B15 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D015234:HLA-A Antigens; C503735:HLA-A*02:61 antigen; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; C503736:HLA-B*15:85 antigen; C503737:HLA-B*15:87 antigen; D059910:HLA-B15 Antigen