[Regulatory effects of micronutrient complex on the expression of Th1 and Th2 cytokines in diabetic C57BL mice].
OBJECTIVE
To investigate the regulatory effects of micronutrients complex (MC) on the expression of Th1 and Th2 cytokines in diabetic mice for exploring the molecular mechanisms of MC in treating for diabetes.


METHODS
IDDM mice model was made by the injection of multiple low dose of streptozotocin (MLDS). The composition of Selenium (Se), Vitamin E (VE), Vanadium (V) and Chrome (Cr) was supplemented. The percentage of Th1 cytokines(TNF-alpha, IFN-gamma) and Th2 cytokines (IL-4, IL-10) positive lymphocytes were detected by flow cytometer (FCM).


RESULTS
The expression of TNF-alpha and IFN-gamma of peripheral blood lymphocytes of MLDS group significantly increased (P < 0.01), while the IL-10 expression of blood and spleen lymphocytes of MLDS group obviously decreased (P < 0.01). Combined supplementation of MC markedly decreased blood lymphocytes TNF-alpha expression (P < 0.01) and increased blood lymphocytes IL-10 expression (P < 0.01) and spleen lymphocytes IL-4 expression (P < 0.05) of IDDM mice respectively.


CONCLUSIONS
MC may prevent from the onset and development of IDDM by down-regulating Th1 cytokines genes expression and up-regulating Th2 cytokines genes expression of IDDM mice.
Chang
Ying
Y
Central Research Department, China-Japan Union Hospital, Jilin University, Changchun 130031, China.
Piao
Song-Lan
SL
Gao
Shen
S
Zheng
De-Ming
DM
D000818:Animals; D002857:Chromium / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018977:Micronutrients / Q000494:pharmacology*; D011897:Random Allocation; D012643:Selenium / Q000494:pharmacology; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*; D014639:Vanadium / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D016207:Cytokines; D018977:Micronutrients; D014639:Vanadium; D002857:Chromium; D014810:Vitamin E; D012643:Selenium