[Ribosomal protein genes highly expressed in swamp eel gonads].
8 cDNA clones have been isolated from a cDNA library prepared from swamp eel testies by macroarray. DNA sequence analysis and database search showed that they encode 8 proteins which are highly homologous to 40S ribosomal proteins S4,S9,S16,S17,S20 and 60S riobosomal proteins L7, L18a,L29. Phylogenetic trees (ML) based on ribosomal protein genes from swamp eel and other organisms has been reconstructed, which showed that ribosomal protein genes were highly conserved during evolution. These results suggested that ribosomal protein genes as house keeping genes may play roles in developmental regulation such as sexual differentiation and can also be used as markers for the study of molecular evolution.
Shang
Xuan
X
Center for Developmental Biology ,College of Life Science , Wuhan University, Wuhan 430072, China. shangrabbit@163.com
He
Yan
Y
Zhang
Lei
L
He
Chun-Jiang
CJ
Xia
Lai-Xin
LX
Gao
Shang
S
Guo
Yi-Qing
YQ
Cheng
Han-Hua
HH
Zhou
Rong-Jia
RJ
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006066:Gonads / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000235:genetics*; D054683:Ribosome Subunits, Large, Eukaryotic / Q000235:genetics; D054682:Ribosome Subunits, Small, Eukaryotic / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics; D023701:Smegmamorpha / Q000145:classification / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary; D012269:Ribosomal Proteins