[Experiment study on the estrogen-like effect of compounds of mercury, chromium and manganese].
OBJECTIVE
In order to study estrogen-like effect of compounds of Mercury, Chromium and Manganese.


METHODS
Mercury Chloride, Manganese Sulfate, Chromium Chloride and Chromium trioxide were selected to perform proliferation assay of MCF-7 human breast cancer cells and binding assay of estrogen receptor for rat uterine.


RESULTS
Mercury Chloride could stimulate the proliferation of MCF-7 cell, the maximal increase of MCF-7 cells was measured by Mercury Chloride at 10(-7) mol/L. The effect was blocked completely by a pure antigestrogen ICI182, 780; but Mercury Chloride could not bind to ER by competing with E2. Chromium Chloride, Chromium Trioxide and Manganese Sulfate were not able to stimulate the proliferation of MCF-7 cell and bind to ER through competing against 3H-E2 in vitro.


CONCLUSIONS
Mercury Chloride exhibited the estrogenicity through binding and activating ER; Chromium Chloride, Chromium trioxide and Manganese Sulfate did not show any estrogenicity.
Wang
Ya-Dong
YD
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Chen
Xiao-Yu
XY
Wu
Yi-Ming
YM
Xu
Dong
D
D000818:Animals; D001667:Binding, Competitive; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D017608:Chromium Compounds / Q000494:pharmacology*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004785:Environmental Pollutants / Q000494:pharmacology; D005260:Female; D006801:Humans; D017895:Manganese Compounds / Q000494:pharmacology*; D008627:Mercuric Chloride / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000378:metabolism; D013431:Sulfates / Q000494:pharmacology*
D002712:Chlorides; D017608:Chromium Compounds; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D017895:Manganese Compounds; D011960:Receptors, Estrogen; D013431:Sulfates; D008627:Mercuric Chloride; C028801:chromium trioxide; C017133:chromous chloride; C039798:manganese sulfate