title: Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods.
abstract: Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results in 60 of the 61 isolates.
authors.affiliation: Vaccine Preventable Bacterial Diseases Division, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada. rtsang@hc-sc.gc.ca
authors.forename: Raymond S W
authors.initials: RS
authors.lastname: Tsang
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Sill
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Advani
authors.forename: Dorothy
authors.initials: D
authors.lastname: Xing
authors.forename: Penny
authors.initials: P
authors.lastname: Newland
authors.forename: Hans
authors.initials: H
authors.lastname: Hallander
mesh_terms: D000372:Agglutination Tests / Q000379:methods; D000911:Antibodies, Monoclonal; D001886:Bordetella pertussis / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012703:Serotyping / Q000379:methods; D013548:Sweden; D014917:Whooping Cough / Q000175:diagnosis
chemical_list: D000911:Antibodies, Monoclonal