title: Improvements in europium sensitized fluorimetric determination of demeclocycline and methacycline.
abstract: Demeclocycline (DM) and methacycline (MT) have been determined by europium-sensitized fluorescence, using EDTA as co-ligand and cetyltrimethylammonium chloride as surfactant. The methods have been developed in slightly alkaline solutions, with the formation of a new chelate where the lanthanide ion is bound to the beta-diketone group. Calibration graphs between 0.01 and 0.1 microg mL(-1) have been obtained for DM and MT determination. Both methods have been applied to the determination of these tetracyclines in serum samples with satisfactory recovery results.
authors.affiliation: Department of Analytical Chemistry, University of Extremadura, 06071 Badajoz, Spain. mahedero@unex.es
authors.forename: M C
authors.initials: MC
authors.lastname: Mahedero
authors.forename: D
authors.initials: D
authors.lastname: Bohoyo
authors.forename: F
authors.initials: F
authors.lastname: Salinas
authors.forename: T
authors.initials: T
authors.lastname: Ardila
authors.forename: D
authors.initials: D
authors.lastname: Airado
authors.forename: B
authors.initials: B
authors.lastname: Roldán
mesh_terms: D003707:Demeclocycline / Q000032:analysis*; D005063:Europium / Q000032:analysis*; D008690:Methacycline / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005063:Europium; D003707:Demeclocycline; D008690:Methacycline