[Regulation of soluble TRAIL and membrane TRAIL in Jurkat cells by PMA].
OBJECTIVE
To investigate the regulation of soluble and membrane bound TNF-related apoptosis-inducing ligand (TRAIL) in Jurkt cells by phorbol myristic acctate (PMA), and the cytotoxicity of the two forms of TRAIL.


METHODS
Jurkat cells were cultured in the presence of 40 ng/mL PMA for different time. The production of sTRAIL was determined by ELISA, and expression of mTRAIL was analyzed by indirect fluorescence staining and flow cytometry analysis. The cytotoxicites of sTRAIL and mTRAIL were detected by (51)Cr release assay, in which DR4/DR5-expressing Raji cells were employed as target cells.


RESULTS
The expression of both sTRAIL and mTRAIL in Jurkat cells were upregulated by PMA. The level of sTRAIL in supernatant from PMA-stimulated Jurkat cell culture increased and reached peak at 48 hours after PMA treatment, whereas expression peak of mTRAIL was at 60 hours. Both sTRAIL and mTRAIL exhibited cytotoxicity against Raji cells.


CONCLUSIONS
PMA, a PKC activator, can upregulate the expression of both sTRAIL and mTRAIL in Jurkat cells, and the two forms of TRAIL have cytotoxic activity.
Li
Yan
Y
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Cao
Yun-xin
YX
Zhang
Yun
Y
Song
Chao-jun
CJ
Zhuang
Ran
R
Fang
Liang
L
Jin
Bo-quan
BQ
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism* / Q000633:toxicity; D051379:Mice; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D012995:Solubility; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D008565:Membrane Proteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand; D013755:Tetradecanoylphorbol Acetate