Influence of hepatocyte growth factor/scatter factor (HGF/SF) on fibroblast growth factor-2 (FGF-2) levels in external auditory canal cholesteatoma (EACC) cell culture.
BACKGROUND
In previous studies, we cited circulatory disorders and hypoxia as etiological factors for the formation of external auditory canal cholesteatoma (EACC) resulting in angiogenesis. Here, we investigate how the angiogenic factor hepatocyte growth factor/scatter factor (HGF/SF) influences the level of another angiogenic factor FGF-2.


METHODS
After 16 to 72 hours of incubation with 20 ng/ml HGF/SF, levels of VEGF in the HGF/SF-treated and untreated culture was analyzed. We also investigated the influence of HGF/SF (20-80 ng/ml) on the concentration of FGF-2.


RESULTS
After 16 hours of incubation with HGF/SF at 20 ng/ml, FGF-2 was measured at 44.19 pg/ml (control: 42.24 pg/ml). After 72 hours, FGF-2 was at 23.41 pg/ml (control: 14.83 pg/ml). After 24 hours, 40 ng/ml HGF/SF showed the highest concentration of FGF-2.


CONCLUSIONS
FGF-2 levels were initially elevated after treatment with HGF/SF, however, further incubation did not show any increase. We assume that HGF/SF released FGF-2 in the matrix but did not induce FGF-2 expression. The most effective HGF/SF concentration was 40 ng/ml.
Naim
Ramin
R
Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, D-68135 Mannheim, Germany. ramin.naim@hno.ma.uni-heidelberg.de
Chang
Ray C
RC
Sadick
Haneen
H
Hormann
Karl
K
D042501:Angiogenic Proteins / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D018424:Cholesteatoma, Middle Ear / Q000503:physiopathology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D009389:Neovascularization, Pathologic; D012867:Skin / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D042501:Angiogenic Proteins; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor