title: Tissue distribution, synthesis stage, and ethylene induction of pineapple (Ananas comosus) chitinases.
abstract: We examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. Type A (acidic class III) exists in all tissues, while type B (weakly basic class I, which has strong antifungal activity) and type C (acidic class I) are localized mainly in the leaf and stem. In a pericarp, type A exists at all stages during fruit development, while type B and type C exist only at the early stage. Synthesis of type A is induced by ethylene, while that of types B and C is not affected by it. These results suggest that the physiological roles of these three types of chitinase in pineapple are different.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University, Okinawa 903-0213, Japan. tokey@agr.u-ryukyu.ac.jp
authors.forename: Toki
authors.initials: T
authors.lastname: Taira
authors.forename: Noriko
authors.initials: N
authors.lastname: Toma
authors.forename: Marika
authors.initials: M
authors.lastname: Ichi
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Ishihara
mesh_terms: D031230:Ananas / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D002688:Chitinases / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D005638:Fruit / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant
chemical_list: D005030:Ethylenes; C036216:ethylene; D002688:Chitinases