title: [The effect of the extract from Ranunculus japonicus on [Ca2+]i inside rabbit VSMC by serologic pharmacological test].
abstract: OBJECTIVE
  To observe the influence of serum containing the extract from Ranunculus japonicus (ERj) on the amount of [Ca2+]i inside vascular smooth muscle cells (VSMC).
  
  
  METHODS
  Serum containing ERj was prepared by serum pharmacological method. Cultured VSMCs induced by NE were treated with the serum, and the influence of it on the amount [Ca2+]i inside cells indicated with INDO-AM was determined.
  
  
  RESULTS
  The serum containing ERj could markedly decrease the increase of the amount of free [Ca2+]i induced by NE in cultured VSMC.
  
  
  CONCLUSIONS
  The serum containing ERj could decrease the intracellular [Ca2+]i.
authors.affiliation: Department of Pharmacy, Guangdong College of Pharmacy, Guangzhou 510224.
authors.forename: Sa
authors.initials: S
authors.lastname: Cai
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005456:Fluorescent Dyes; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D031947:Ranunculus / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D002118:Calcium