The cell surface receptor G6b, a member of the immunoglobulin superfamily, binds heparin.
The G6b gene, located in the human Major Histocompatibility Complex, encodes a receptor of the immunoglobulin (Ig) superfamily. In this study, we show using a variety of techniques that the extracellular domain of the G6b protein, containing a single Ig-like domain, binds to heparin with high affinity. In an ELISA assay, this binding was displaceable with soluble heparin with an IC50 value of approximately 0.5 microg/ml. Other sulfated glycans showed weaker or no competition. The observed interaction between G6b and heparin is strongly salt dependent suggesting a mainly electrostatic interaction. Heparin might modulate the interaction of G6b with its as yet unidentified protein ligand.
de Vet
Edwin C J M
EC
MRC Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, UK.
Newland
Stephen A B
SA
Lyons
Paul A
PA
Aguado
Begoña
B
Campbell
R Duncan
RD
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005803:Genes, Immunoglobulin; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008024:Ligands; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives*; D012995:Solubility; D055672:Static Electricity
D007141:Immunoglobulin Fc Fragments; D008024:Ligands; C119883:MPIG6B protein, human; D011971:Receptors, Immunologic; C037405:heparin-sepharose; D006493:Heparin; D012685:Sepharose