Leptosphaeria rhodopsin: bacteriorhodopsin-like proton pump from a eukaryote.
Bacteriorhodopsin-like proteins provide archaea and eubacteria with a unique bioenergetic pathway comprising light-driven transmembrane proton translocation by a single retinal-binding protein. Recently, homologous proteins were found to perform photosensory functions in lower eukaryotes, but no active ion transport by eukaryotic rhodopsins was detected. By demonstrating light-driven proton pumping in a fungal rhodopsin from Leptosphaeria maculans, we present a case of a retinal-based proton transporter from a eukaryote. This result implies that in addition to oxidative phosphorylation and chlorophyll photosynthesis, some lower eukaryotes may have retained the archaeal route of building an electrochemical transmembrane gradient of protons.
Waschuk
Stephen A
SA
Department of Physics, University of Guelph, Guelph, ON, Canada N1G 2W1.
Bezerra
Arandi G
AG
Shi
Lichi
L
Brown
Leonid S
LS
D001203:Ascomycota / Q000378:metabolism*; D001436:Bacteriorhodopsins / Q000737:chemistry*; D002734:Chlorophyll / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D007834:Lasers; D008027:Light; D008081:Liposomes / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D010843:Pichia / Q000378:metabolism; D011485:Protein Binding; D017494:Proton Pumps; D011522:Protons; D012243:Rhodopsin / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013997:Time Factors
D007477:Ions; D008081:Liposomes; D017494:Proton Pumps; D011522:Protons; D002734:Chlorophyll; D001436:Bacteriorhodopsins; D012243:Rhodopsin; D010100:Oxygen