title: Lipid peroxidation during ischemia depends on ischemia time in warm ischemia and reperfusion of rat liver.
abstract: Prolonged hepatic warm ischemia has been incriminated in oxidative stress after reperfusion. However, the magnitude of oxidative stress during ischemia has been controversial. The aims of the present study were to elucidate whether lipid peroxidation progressed during ischemia and to clarify whether oxidative stress during ischemia aggravated the oxidative damage after reperfusion. Rats were subjected to 30 to 120 min of 70% warm ischemia alone or followed by reperfusion for 60 min. Lipid peroxidation (LPO) was evaluated by amounts of phosphatidylcholine hydroperoxide (PC-OOH) and phosphatidylethanolamine hydroperoxide (PE-OOH) as primary LPO products. Total amounts of malondialdehyde and 4-hydroxy-2-nonenal (MDA + 4-HNE), degraded from hydroperoxides, were also determined. PC-OOH and PE-OOH significantly increased at 60 and 120 min ischemia with concomitant increase of oxidized glutathione. These hydroperoxides did not increase at 60 min reperfusion after 60 min ischemia, whereas they did increase at 60 min reperfusion after 120 min ischemia with deactivation of phospholipid hydroperoxide glutathione peroxidase and superoxide dismutase. The amount of MDA + 4-HNE exhibited similar changes, but the velocity of production dropped with ischemic time longer than 60 min. In conclusion, oxidative stress progressed during ischemia and triggered the oxidative injury after reperfusion. Secondary LPO products are less sensitive, especially during ischemia, which may cause possible underestimation and discrepancy.
authors.affiliation: First Department of Surgery, Hokkaido University School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Moto
authors.initials: M
authors.lastname: Fukai
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Yokota
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimamura
authors.forename: Tomomi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Michiaki
authors.initials: M
authors.lastname: Matsushita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furukawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Todo
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008111:Liver Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism; D015996:Survival Rate
chemical_list: D000447:Aldehydes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C057590:phosphatidylcholine hydroperoxide; C027850:phosphatidylethanolamine hydroperoxide; D008315:Malondialdehyde; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C027576:4-hydroxy-2-nonenal