title: Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection.
abstract: In previous studies, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) proteins induced specific humoral and cellular immune responses in susceptible and resistant mice in the absence of Trypanosoma cruzi infection with a significant induction of the Interferon-gamma (IFN-gamma) production in those animals. In this follow-up paper, the immunostimulatory and protective effects of these proteins were evaluated by immunizing with CRA or FRA antigens, BALB/c and C57BL/6 mice and challenging with a T. cruzi (Y strain). Both proteins induced humoral response with high levels of IgG isotypes as well as cellular immunity with high levels of IFN-gamma when compared to controls. However, the lymphocyte proliferative response was minimal. The survival rate at 30 days post-infection was significant in CRA (60%) or FRA (50%)--immunized BALB/c mice and CRA (83.3%)--immunized C57BL/6 mice. Taken as a whole these findings indicate that CRA and FRA are immunogenic and potentially important for protective immunity.
authors.affiliation: Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil.
authors.forename: Valéria R A
authors.initials: VR
authors.lastname: Pereira
authors.forename: Virginia M B
authors.initials: VM
authors.lastname: Lorena
authors.forename: Mineo
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Luna
authors.forename: Edimilson D
authors.initials: ED
authors.lastname: Silva
authors.forename: Antonio G P
authors.initials: AG
authors.lastname: Ferreira
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Krieger
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.forename: Milena B P
authors.initials: MB
authors.lastname: Soares
authors.forename: Eridan M
authors.initials: EM
authors.lastname: Coutinho
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Correa-Oliveira
authors.forename: Yara M
authors.initials: YM
authors.lastname: Gomes
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C060967:cytoplasmic antigen, Trypanosoma; D007371:Interferon-gamma