title: Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4.
abstract: Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonas paucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2,3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type metapathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas.
authors.affiliation: College of Life Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
authors.forename: Hang
authors.initials: H
authors.lastname: Min
authors.forename: Gang
authors.initials: G
authors.lastname: Rao
authors.forename: Zhen-mei
authors.initials: ZM
authors.lastname: Lv
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.forename: Yang-fang
authors.initials: YF
authors.lastname: Ye
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Duan
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010616:Phenanthrenes; D011506:Proteins; D012989:Soil Pollutants; C031181:phenanthrene; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D005982:Glutathione Transferase; C092541:2-hydroxymuconate-semialdehyde hydrolase