Tumour therapy with genes encoding apoptin and E4orf4.
The aim of our study was to investigate whether apoptin and e4orf4 pro-apoptotic genes, transferred by means of electroporation, were suitable for gene therapy of tumours. The two genes were chosen for our study because the proteins they encode induce apoptosis in transformed cells only. The apoptin gene was synthesised based on a published nucleotide sequence. MTT and TUNEL tests confirmed that both the synthesised apoptin gene and the e4orf4 gene indeed induced apoptosis in COS-7, Renca and B16(F10) cell lines. Therapeutic DNA was then administered via electroporation directly into murine B16(F10) tumours. Distinct tumour growth inhibition was seen only during the treatment. The cessation of therapy caused tumour re-growth. Obviously, the efficiency of gene transfer using electroporation is low and did not induce a permanent therapeutic effect.
Mitrus
Iwona
I
Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101 Gliwice, Poland.
Missol-Kolka
Ewa
E
Plucienniczak
Andrzej
A
Szala
Stanislaw
S
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D019556:COS Cells; D036022:Capsid Proteins / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000628:therapy; D002522:Chlorocebus aethiops; D018274:Electroporation; D015316:Genetic Therapy / Q000379:methods*; D020287:In Situ Nick-End Labeling; D007680:Kidney Neoplasms / Q000235:genetics / Q000628:therapy; D008546:Melanoma, Experimental / Q000235:genetics / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000628:therapy*; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; C121517:E4orf4 protein, adenovirus; C091717:VP3 protein, Chicken anemia virus; D014764:Viral Proteins