title: Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies.
abstract: In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.
authors.affiliation: Department of Immunology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kaji
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Akagawa
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Taniyama
authors.forename: Masataka
authors.initials: M
authors.lastname: Kasai
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Tsunetsugu-Yokota
authors.forename: Masamichi
authors.initials: M
authors.lastname: Oshima
authors.forename: Kiichi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Naomi
authors.initials: N
authors.lastname: Takasuka
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Ato
authors.forename: Hideki
authors.initials: H
authors.lastname: Fujii
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Ishii
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Takagi
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Itamura
authors.forename: Takato
authors.initials: T
authors.lastname: Odagiri
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Miyamura
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshikura
authors.forename: Toshitada
authors.initials: T
authors.lastname: Takemori
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis*; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal