[Research on gene transfer by spermatozoa].
Incubate golden hamster spermatozoa and NF (kappa) B-luc(+) in co-culture and liposome mediated methods, and then process the IVF with oocytes, And two groups of samples were detected by PCR, Southern Blot and FISH. Analyzed the relative data by statistics. The results showed that spermatozoa of Golden hamster epididymis can take the foreign DNA voluntarily, and the foreign DNA exist in the head of the spermatozoa can enter into zygotes by IVF. The exist of liposome can improve the foreign gene transfect ratio and transgenic ratio.
Wang
Xiao-Mei
XM
School of Public Health, Lanzhou University, Lanzhou 730000, China. xmwang@szu.edu.cn
Ruan
Ye
Y
Xie
Qing-Dong
QD
Huang
Tian-Hua
TH
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D018920:Coculture Techniques; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017404:In Situ Hybridization, Fluorescence; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013077:Sperm Head / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D015053:Zygote / Q000166:cytology / Q000378:metabolism
D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D008156:Luciferases