[Study of mechanism on immune response in goats by different immunization with Helicobacter pylori and analyse of its prospects for using].
OBJECTIVE
To observe the effect of three different routes on the induction of immunogenicity to whole bacterial antigen of Vac A+ Helicobacter pylori strain (NCTC11637) and explore its mechanism.


METHODS
Two milk goats and one pregnant goat were immunized by different routes respectively with whole bacterial of H. pylori (6 x 10(9) cfu/ml) cultivated by solid culture medium. At the 1st, 14th, 21st, 28th day, two goats were immunized four times by intranasal or subcutaneous injection. The other pregnant goat was immunized four times at a interval of two weeks before and after one month of lamb birth by muscular injection. Serum and milk samples were collected and assayed by indirect enayme-linked immunosorbent assay (ELISA). The levels of anti-Hp of IgG and IgA in serum and milk were determined by reading the optical density (A).


RESULTS
Three immune routes all induced systemic immune response. The optical density (A) of ELISA proved that the specific IgG in serum increased while IgA didn't increase significantly, and that the specific IgA and IgG in milk all increased to a greater or lesser extent compared with control group.


CONCLUSIONS
Three immune routes all induce systemic immune response, resulting in increases of anti-Hp of IgG/IgA in milk and IgG in serum. Among them, intranasal inoculation can induce systemic immune response and local immune response in different mucosal sites, which may be a safe and effective immunization route.
Zhang
Tian-Zhe
TZ
North China Coal Medical College, Tangshan 063000, China.
Yuan
Ju-Xiang
JX
Xu
Ying-Jun
YJ
Zhang
Tie-Min
TM
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D008892:Milk / Q000276:immunology; D011247:Pregnancy
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G