Assessment of mumps virus growth on various continuous cell lines by virological, immunological, molecular and morphological investigations.
Vero cells have been used as a convenient laboratory substrate for the isolation of mumps virus but may not be very sensitive and may select for particular adapted variants from clinical specimens. Continuous cell lines were evaluated for their ability to support the replication of mumps virus. Criteria included the production of infectious virus, detection of intracellular mumps proteins by immunofluorescence and electron microscopy and detection of specific nucleic acid by RT-PCR. Of the cells tested, CaCo-2, PLC/PRF/5, and Vero cells produced infectious virus, with Vero and CaCo-2 being the most permissive. The other substrates tested included cells of murine, canine and human origin showed signs of intracellular proteins and RNA but the amounts produced were much lower, and no infectious virus was detected in some cases. The virus use was a low passage of a Vero derived wild type strain, and it will ultimately be necessary to continue the studies with an unpassaged clinical specimen to identify a cell line able to isolate mumps virus at high efficiency and in unmodified form.
Afzal
M A
MA
Division of Virology, National Institute for Biological Standards and Control, Blanch lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK. mafzal@nibsc.ac.uk
Dussupt
V
V
Minor
P D
PD
Pipkin
P A
PA
Fleck
R
R
Hockley
D J
DJ
Stacey
G N
GN
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009109:Mumps virus / Q000254:growth & development* / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis*
D012367:RNA, Viral; D014764:Viral Proteins