The effects of estradiol and progesterone on the synthesis of collagen in tracheal surgery.
OBJECTIVE
Estrogen and progesterone have the decreasing effect on massive collagen synthesis in wound healing. Here, it is aimed to determine their decreasing effect on collagen accumulation and fibroblast proliferation in trachea histologically and to understand if they would be protective for tracheal stenosis.


METHODS
Thirteen male Winstar rats were divided randomly into two groups: estrogen-progesterone group (group 1, eight rats) and control group (group 2, five rats). Under general anesthesia, tracheas were incised vertically extending from second to fifth tracheal ring. Incision was closed with absorbable sutures. Estrodiol benzoat and progesterone was given intramuscularly to estrogen progesterone group, saline solution to control group. After 4 weeks they were sacrificed and tracheas were excised. Horizontal cross section of the narrowest part of the incised trachea was examined histologically. Epithelial regeneration, inflammatory cell infiltration, angiogenesis, fibroblast proliferation and collagen deposition were evaluated by histological grading.


RESULTS
Statistically significant difference was found between the groups in collagen deposition and fibroblast proliferation (p=0.011, <0.05). For epithelial regeneration, inflammatory cell infiltration, and angiogenesis there was no difference.


CONCLUSIONS
As a result, it was proved that sex hormones inhibit massive collagen deposition and fibroblast proliferation in wound healing of tracheal surgery. Hence, they may prevent tracheal stenosis.
Liman
Serife Tuba
ST
Thoracic Surgery Department, Kocaeli University, The Faculty of Medicine, Kocaeli, Turkey. tuba.liman@superonline.com
Kara
Cuneyt Orhan
CO
Bir
Ferda
F
Yildirim
Basak
B
Topcu
Salih
S
Sahin
Barbaros
B
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D003251:Constriction, Pathologic; D004958:Estradiol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D014132:Trachea / Q000293:injuries* / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*
D012739:Gonadal Steroid Hormones; D011374:Progesterone; D004958:Estradiol; D003094:Collagen