Suppression of proliferation of poliovirus and porcine parvovirus by novel phenoxazines, 2-amino-4,4 alpha-dihydro-4 alpha-7-dimethyl-3H-phenoxazine-3-one and 3-amino-1,4 alpha-dihydro-4 alpha-8-dimethyl-2H-phenoxazine-2-one.
The present study aimed at investigating the antiviral effects of 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) and 3-amino-1,4alpha-dihydro-4alpha-8-dimethyl-2H-phenoxazine-2-one (Phx-2) on 6 representative viruses: poliovirus, porcine parvovirus, simian virus 40 (SV-40), herpes simplex virus-1 (HSV-1), Sindbis virus, and vesicular stomatitis virus (VSV). Phx-1 and Phx-2 suppressed the proliferation of poliovirus in Vero cells and that of porcine parvovirus in ESK cells at concentrations between 0.25 microg/ml and 2 microg/ml, when the cells were treated with Phx-1 and Phx-2 for 1 h and then inoculated with these viruses. The proliferation of the other viruses, SV-40, HSV-1, Sindbis virus, and VSV, in the host cells was not influenced by Phx-1 or Phx-2 at concentrations less than 20 microg/ml. The results suggest that Phx-1 and Phx-2 may be useful to prevent the proliferation of poliovirus and porcine parvovirus infection and may contribute to developing new antiviral drugs in future.
Iwata
Akiko
A
Division of Cellular and Gene Therapy Products, National Institute of Health and Sciences, Japan, Tokyo 058-0098, Japan.
Yamaguchi
Teruhide
T
Sato
Kouei
K
Yoshitake
Noriko
N
Tomoda
Akio
A
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008253:Macaca mulatta; D010078:Oxazines / Q000737:chemistry / Q000494:pharmacology*; D017992:Parvovirus / Q000187:drug effects* / Q000254:growth & development*; D017955:Poliovirus / Q000187:drug effects* / Q000254:growth & development; D013539:Simian virus 40; D013552:Swine; D014709:Vero Cells
D000998:Antiviral Agents; D010078:Oxazines; C039203:phenoxazine