title: Response of human pulp capped with a bonding agent after bleeding control with hemostatic agents.
abstract: OBJECTIVE
  This study evaluated the response of human pulps capped with a bonding agent after bleeding control with different hemostatic agents.
  
  
  METHODS
  Twenty-five Class II cavities were prepared in 25 caries-free human premolars scheduled for extraction due to orthodontic treatment. The pulp exposures were performed on the occlusal floor. The teeth were randomly divided into five groups. Groups 1-4 were capped with an adhesive system after hemostasis with different agents: Group 1--saline solution; 2--ferric sulfate; 3--2.5% NaOCl; 4--Ca(OH)2 solution. In Group 5, after hemostasis with saline solution, the pulp was capped with calcium hydroxide (control group). Then, ScotchBond Multi Purpose Plus was applied and the resin composite Z-100 placed incrementally according to the manufacturers' directions. After 60 days, the teeth were extracted and processed for light microscopic examination (HE) and the groups were categorized in a histological score system. The data were subjected to a non-parametric test (alpha=0.05).
  
  
  RESULTS
  Overall, the histological features showed that the pulp response from Groups 1 through 4 was inferior to the response from Group 5, where dentin bridging occurred. In all groups, where the adhesive system was used for capping, the pulp response varied from an acute inflammatory, with varying degrees, to necrosis. No dentin bridge was formed after adhesive capping.
authors.affiliation: Department of Dental Materials, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Maria de Lourdes Rodrigues
authors.initials: Mde L
authors.lastname: Accorinte
authors.forename: Alessandro Dourado
authors.initials: AD
authors.lastname: Loguercio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reis
authors.forename: Antonio
authors.initials: A
authors.lastname: Muench
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: de Araújo
mesh_terms: D000293:Adolescent; D000328:Adult; D001252:Astringents / Q000627:therapeutic use; D001641:Bicuspid; D002126:Calcium Hydroxide / Q000627:therapeutic use; D003188:Composite Resins / Q000737:chemistry; D003737:Dental Cavity Preparation / Q000145:classification; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D003785:Dental Pulp Capping / Q000379:methods*; D003790:Dental Pulp Necrosis / Q000473:pathology; D003793:Dental Restoration, Permanent; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005290:Ferric Compounds / Q000627:therapeutic use; D006490:Hemostatics / Q000627:therapeutic use*; D006801:Humans; D011671:Pulpitis / Q000473:pathology; D019279:Resin Cements / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012965:Sodium Chloride; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects; D015040:Zirconium / Q000737:chemistry
chemical_list: D001252:Astringents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005290:Ferric Compounds; D006490:Hemostatics; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C083568:Z100 composite resin; C024823:ferric sulfate; D012965:Sodium Chloride; D012822:Silicon Dioxide; D015040:Zirconium; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide