8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage induced by environmental tobacco side-stream smoke and its mechanism.
OBJECTIVE
To study the genotoxicity effect of environmental tobacco side-stream smokes (ETSS) on oxidative DNA damage and its molecular mechanism.


METHODS
DNA adduct 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. The level of 8-OHdG in DNA exposed to ETSS was detected by high performance liquid chromatography with electrochemical detection. Organic and inorganic components in ETSS were analyzed by gas chromatography-mass spectrum and atomic absorption spectrum respectively.


RESULTS
Particle matters (PMs) and volatile organic compounds (VOCs) in ETSS could directly induce oxidative DNA damage and formation of 8-OHdG. There were 123 and 84 kinds of organic components in PMs and VOCs respectively, and 7 kinds of inorganic components in ETSS. Some components, especially quinones and polyphenols in ETSS, could produce free radicals in vitro by auto-oxidation without any biological activity systems, and with the catalytic reaction of metals, the DNA adduct 8-OHdG was produced.


CONCLUSIONS
ETSS have biological oxidative effect on DNA in vitro and in vivo, and expressed direct genotoxicity. 8-OHdG is a valuable biomarker of oxidative DNA damage.
Xi
Zhu-Ge
ZG
Institute of Hygiene and Environmental Medicine, Tianjin 300050, China.
Chao
Fu-Huan
FH
Yang
Dan-Feng
DF
Zhang
Hua-Shan
HS
Zhang
Wei
W
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D010084:Oxidation-Reduction; D051381:Rats; D014028:Tobacco Smoke Pollution / Q000009:adverse effects* / Q000032:analysis
D015415:Biomarkers; D018736:DNA Adducts; D019216:Metals, Heavy; D009930:Organic Chemicals; D014028:Tobacco Smoke Pollution; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; C427203:calf thymus DNA; D003849:Deoxyguanosine