title: Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis.
abstract: Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.
authors.affiliation: Laboratory of Nematology, Graduate School for Experimental Plant Sciences, Wageningen University, The Netherlands.
authors.forename: Urszula
authors.initials: U
authors.lastname: Kudla
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Adina
authors.initials: A
authors.lastname: Milac
authors.forename: Anna
authors.initials: A
authors.lastname: Kielak
authors.forename: Cyril
authors.initials: C
authors.lastname: Maissen
authors.forename: Hein
authors.initials: H
authors.lastname: Overmars
authors.forename: Herman
authors.initials: H
authors.lastname: Popeijus
authors.forename: Erwin
authors.initials: E
authors.lastname: Roze
authors.forename: Andrei
authors.initials: A
authors.lastname: Petrescu
authors.forename: Geert
authors.initials: G
authors.lastname: Smant
authors.forename: Jaap
authors.initials: J
authors.lastname: Bakker
authors.forename: Johannes
authors.initials: J
authors.lastname: Helder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D016678:Genome; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000469:parasitology*; D014026:Nicotiana / Q000235:genetics; D017187:Tylenchida / Q000737:chemistry* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D015801:Helminth Proteins