title: Functional dissociation of the basolateral transcytotic compartment from the apical phago-lysosomal compartment in human osteoclasts.
abstract: Tartrate-resistant acid phosphatase (TRAP) is essential for elimination of Staphylococcus aureus, the main infectious agent responsible for osteomyelitis. This in vitro study investigated uptake and processing of fluorescence-labeled S. aureus by human osteoclasts and dendritic cells. The cells were stained for TRAP and the acidic compartment using a fluorescence-based protocol. In dendritic cells, TRAP and bacteria were colocalized. In osteoclasts, there was no colocalization of bacteria, TRAP, or the acidic compartment, indicating that there are three distinct vesicular compartments: the apical phago-lysosomal compartment, the basal secretory compartment, and the basolateral transcytotic compartment. Dissociation of the TRAP-containing transcytotic vesicles from the apical phago-lysosomal compartment may restrain osteoclasts from eliminating S. aureus.
authors.affiliation: School of Anatomy and Human Biology, The University of Western Australia, Crawley WA 6009, Australia.
authors.forename: James
authors.initials: J
authors.lastname: Meagher
authors.forename: René
authors.initials: R
authors.lastname: Zellweger
authors.forename: Luis
authors.initials: L
authors.lastname: Filgueira
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure; D004705:Endocytosis; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008247:Lysosomes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010010:Osteoclasts / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D010586:Phagocytes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D013211:Staphylococcus aureus / Q000502:physiology*; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase