title: [The research on the quenching of fluorescence spectra of TOP I by iodine anion].
abstract: Comparing the fluorescence spectra of tobacco peroxidase I (TOP I) solution and the solutions titrated by iodine anions, the number of binding locus and the binding constant of iodine anion to TOP I were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOP I molecule were discussed.
authors.affiliation: Department of Chemistry, USTC, Hefei 230026, China.
authors.forename: Bing-le
authors.initials: BL
authors.lastname: Xia
authors.forename: Dun-geng
authors.initials: DG
authors.lastname: Peng
authors.forename: Min-li
authors.initials: ML
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.forename: Kai-bao
authors.initials: KB
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000838:Anions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007455:Iodine / Q000737:chemistry; D007700:Kinetics; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012107:Research Design; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology*
chemical_list: D000838:Anions; D010940:Plant Proteins; D007455:Iodine; D009195:Peroxidase