title: Quantification of SSX mRNA expression in human bone and soft tissue tumors using nucleic acid sequence-based amplification.
abstract: The SSX family proteins have been considered new members of the cancer/testis antigens because of the restricted expression in testis among normal tissues and the activation in a wide range of cancers. Thus, they would be potential molecular targets for immunotherapeutic strategies. We have developed a competitive nucleic acid sequence-based amplification (NASBA) assay to analyze SSX mRNA expression in 211 bone and soft tissue tumors. The copy numbers of SSX mRNA per mug of total RNA in tumor tissues were widely distributed, ranging logarithmically from 0.6 to 6.6. We found that malignant tumors showed significantly higher expression of SSX mRNA than benign tumors (P < 0.0001). Further, SSX mRNA expression in stage III tumors was significantly higher than that in stage I or II tumors (P < 0.005). This NASBA assay was also more sensitive compared to immunohistochemistry using newly affinity-purified polyclonal antibody against SSX. Collectively, these results suggest that the SSX quantitative NASBA assay could provide useful information to select eligible patients for SSX-specific cancer vaccines.
authors.affiliation: Department of Orthopedic Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-2 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Naka
authors.forename: Susumu
authors.initials: S
authors.lastname: Joyama
authors.forename: Yoshitane
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ujiiye
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Hayashi
authors.forename: Masako
authors.initials: M
authors.lastname: Kawase
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mano
authors.forename: Shingo
authors.initials: S
authors.lastname: Ishiguro
authors.forename: Akira
authors.initials: A
authors.lastname: Myoui
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ueda
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Araki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Itoh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000276:immunology; D019556:COS Cells; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics*; D021161:Self-Sustained Sequence Replication; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000276:immunology
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012097:Repressor Proteins; C093962:synovial sarcoma X breakpoint proteins