Differential requirements for the chemokine receptor CCR7 in T cell activation during Listeria monocytogenes infection.
Effective priming of T cell responses depends on cognate interactions between naive T cells and professional antigen-presenting cells (APCs). This contact is the result of highly coordinated migration processes, in which the chemokine receptor CCR7 and its ligands, CCL19 and CCL21, play a central role. We used the murine Listeria monocytogenes infection model to characterize the role of the CCR7/CCR7 ligand system in the generation of T cell responses during bacterial infection. We demonstrate that efficient priming of naive major histocompatibility complex (MHC) class Ia-restricted CD8+ T cells requires CCR7. In contrast, MHC class Ib-restricted CD8+ T cells and MHC class II-restricted CD4+ T cells seem to be less dependent on CCR7; memory T cell responses are independent of CCR7. Infection experiments with bone marrow chimeras or mice reconstituted with purified T cell populations indicate that CCR7 has to be expressed on CD8+ T cells and professional APCs to promote efficient MHC class Ia-restricted T cell priming. Thus, different T cell subtypes and maturation stages have discrete requirements for CCR7.
Kursar
Mischo
M
Department of Immunology, Max-Planck Institute for Infection Biology, 10117 Berlin, Germany.
Höpken
Uta E
UE
Koch
Markus
M
Köhler
Anne
A
Lipp
Martin
M
Kaufmann
Stefan H E
SH
Mittrücker
Hans-Willi
HW
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000378:metabolism; D017931:DNA Primers; D005434:Flow Cytometry; D007156:Immunologic Memory / Q000276:immunology; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
C515461:Ccl19 protein, mouse; C515479:Ccl21c protein, mouse; C515371:Ccr7 protein, mouse; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC; D017931:DNA Primers; D054400:Receptors, CCR7; D019707:Receptors, Chemokine