title: [Cell apoptosis and the change of Fas/FasL gene in experimental pulmonary fibrosis].
abstract: OBJECTIVE
  To investigate the changes and significance of cell apoptosis and Fas/FasL gene in pulmonary fibrosis.
  
  
  METHODS
  Forty mice were divided into two groups randomly, each group contained twenty mice. TUNEL, immunohistochemistry and in situ hybridization were used to detect the change of cell apoptosis, Fas/FasL mRNA and protein in mice with pulmonary fibrosis caused by bleomycin.
  
  
  RESULTS
  The apoptosis index of lung cells in the pulmonary fibrosis group (55.3 +/- 12.2) was higher than that of control group (4.7 +/- 1.0, t = 13.06, < 0.01). The expression of Fas/FasL mRNA (175.8 +/- 21.6, 5.2 +/- 1.6) and protein (956 +/- 96, 285 +/- 76) in the pulmonary fibrosis group was higher than that of control group (mRNA: 26.6 +/- 1.9, 0.5 +/- 0.4, t = 21.7, 8.79, all < 0.01; protein: 491 +/- 96, 100 +/- 18, t = 5.03, 12.81, < 0.01).
  
  
  CONCLUSIONS
  The apoptosis index of lung cells, Fas/FasL genes and protein were up-regulated in lung tissue of pulmonary fibrosis, which may play an important role in the development of disease.
authors.affiliation: Institute of Respiratory Disease, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Jin-zhao
authors.initials: JZ
authors.lastname: Li
authors.forename: Yan-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Xian-ming
authors.initials: XM
authors.lastname: Hou
authors.forename: Run-jiang
authors.initials: RJ
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor