title: [Prospects for genetic hearing loss screening: 35delG mutation tracking in a newborn population].
abstract: OBJECTIVE
  To investigate the prevalence of the 35delG mutation in a newborn population, with specific molecular testing, and to evaluate the prospects for genetic neonatal screening for hearing impairment.
  
  
  METHODS
  233 newborn were evaluated at the Hospital de Base de São José do Rio Preto, SP, for molecular analysis of the 35delG mutation in the connexin 26 gene, with the reaction technique in allele-specific polymerase chain reaction, after genomic DNA extraction from umbilical cord blood.
  
  
  RESULTS
  Five heterozygotes were identified, obtaining a prevalence of 2.24% of 35delG mutation carriers in the study population.
  
  
  CONCLUSIONS
  Using the molecular test allowed for the identification of the 35delG mutation in the study population with the possibility of being used as a complement to neonatal audiometric screening as being simple, fast, and easily to perform with low costs.
authors.affiliation: Departamento de Anatomia, Faculdade de Medicina de São José do Rio Preto (FAMERP), SP. vabp@bol.com.br
authors.forename: Vânia B
authors.initials: VB
authors.lastname: Piatto
authors.forename: Camila A
authors.initials: CA
authors.lastname: Oliveira
authors.forename: Fabiana
authors.initials: F
authors.lastname: Alexandrino
authors.forename: Carla J
authors.initials: CJ
authors.lastname: Pimpinati
authors.forename: Edi L
authors.initials: EL
authors.lastname: Sartorato
mesh_terms: D001938:Brazil / Q000453:epidemiology; D000072259:Connexin 26; D017630:Connexins / Q000235:genetics*; D005820:Genetic Testing; D034381:Hearing Loss / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D009154:Mutation / Q000235:genetics*; D015995:Prevalence
chemical_list: D017630:Connexins; D000072259:Connexin 26