title: Lack of association of recipient MCP-1 gene promoter polymorphism with acute graft rejection after orthotopic liver transplantation.
abstract: Acute graft rejection after orthotopic liver transplantation (OLT) is associated with leukocyte infiltration of the graft. Monocyte chemoattractant protein-1 (MCP-1) is a beta-chemokine involved in the attraction and accumulation of mononuclear granulocytes toward sites of inflammation. A biallelic polymorphism (G/A) at position -2518 of the MCP-1 gene has been described. Cells obtained from individuals with the GG or AG genotypes have been found to produce more MCP-1 than those obtained from individuals with the AA genotype. The goal of this study was to assess the possible association between this polymorphism and susceptibility to acute graft rejection after OLT. One hundred fifty Caucasian liver transplant recipients from the South of Spain underwent genotyping using a polymerase chain reaction (PCR) amplification followed by restriction fragment length polymorphism (RFLP). No significant differences were observed when patients with versus without acute rejection episodes were compared for the distribution of -2518 MCP-1 genotypes. The present study supports the lack of involvement of polymorphism at position -2518 (A/G) of the MCP-1 gene on the susceptibility to acute allograft rejection among OLT recipients.
authors.affiliation: Servicio de Inmunología, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Franco-López
authors.forename: M F
authors.initials: MF
authors.lastname: González-Escribano
authors.forename: I
authors.initials: I
authors.lastname: Aguilera
authors.forename: J M
authors.initials: JM
authors.lastname: Pascasio
authors.forename: F
authors.initials: F
authors.lastname: Pareja
authors.forename: A
authors.initials: A
authors.lastname: Bernardos
authors.forename: A
authors.initials: A
authors.lastname: Núñez-Roldán
mesh_terms: D001483:Base Sequence; D018932:Chemokine CCL2 / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006084:Graft Rejection / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D016031:Liver Transplantation / Q000473:pathology*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D017931:DNA Primers; D004247:DNA