An NMR-based docking model for the physiological transient complex between cytochrome f and cytochrome c6.
The physiological transient complex between cytochrome f (Cf) and cytochrome c(6) (Cc(6)) from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by NMR spectroscopy. The binding constant at low ionic strength is 8 +/- 2 mM(-1), and the binding site of Cc(6) for Cf is localized around its exposed haem edge. On the basis of the experimental data, the resulting docking simulations suggest that Cc(6) binds to Cf in a fashion that is analogous to that of plastocyanin but differs between prokaryotes and eukaryotes.
Díaz-Moreno
Irene
I
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Spain.
Díaz-Quintana
Antonio
A
Ubbink
Marcellus
M
De la Rosa
Miguel A
MA
D045365:Cytochromes c6 / Q000378:metabolism*; D045348:Cytochromes f / Q000378:metabolism*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular
D045365:Cytochromes c6; D045348:Cytochromes f