Immunophenotypic characterization of high grade pleomorphic sarcomas: a demographic and immunohistochemical study in a major referral center of Pakistan.
OBJECTIVE
Immunophenotypic characterization of high grade (pleomorphic) sarcomas and determination of their frequency, mean/median age, sex preferences and common sites in Pakistani patients.


METHODS
This study included 134 consecutive cases of high grade (pleomorphic) sarcomas diagnosed in adults above the age of 15 years in the section of histopathology at theAga Khan University Hospital, Karachi during a period of two years. These high grade (pleomorphic) sarcomas were immunophenotyped using a panel including antibodies against Vimentin, Desmin, Smooth muscle Actin, S 100, CD34, CD68 and Cytokeratin etc by indirect immunoperoxidase.


RESULTS
Of the 134 cases which were characterized, 38.1% were pleomorphic leiomyosarcoma, followed by pleomorphic rhabdomyosarcoma 14.9%, Malignant Peripheral Nerve Sheath Tumour 9%, pleomorphic liposarcoma 3.7% and pleomorphic storiform Malignant Fibrous Histiocytoma 0.7%. Thirty three percent of cases could not be characterized further. Mean/ median age for Leiomyosarcoma was 50/50, for Rhabdomyosarcomas 33/22, for MPNST 42/41, for Liposarcoma 52/50 and for Malignant Fibrous Histiocytoma 46/46 respectively. The commonest site for leiomyosarcoma was lower limb (43%), for rhabdomyosarcoma head and neck (42%), for MPNSTthorax (36.4%) and for liposarcoma abdomen (50%).


CONCLUSIONS
It was concluded that the most common pleomorphic sarcoma occurring in our adult population was Leiomyosarcoma, and that immunohistochemical stains are essential in most cases for further characterization of pleomorphic high grade sarcoma.
Shah
Hamidullah
H
Department of Pathology and Microbiology, Aga Khan University Hospital, Karachi.
Pervez
Shahid
S
D000328:Adult; D017677:Age Distribution; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis; D003430:Cross-Sectional Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007890:Leiomyosarcoma / Q000473:pathology; D008080:Liposarcoma / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics / Q000473:pathology; D010154:Pakistan; D010641:Phenotype; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000473:pathology; D012509:Sarcoma / Q000235:genetics / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology*; D011795:Surveys and Questionnaires
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor