title: Reintroduction of the 2G12 epitope in an HIV-1 clade C gp120.
abstract: Many clade C isolates of HIV-1 do not react with monoclonal antibody (MAb) 2G12, a broad-ranging human neutralizing MAb that recognizes high mannose carbohydrate groups attached to glycoprotein gp120. We reintroduced a partial and complete 2G12 epitope into a clade C background, HIV-1(CN54), and examined the antibody reactivity of the resulting recombinant molecules. Two glycosylation sites recovered 2G12 binding completely, but some binding was evident after the reintroduction of a single glycosylation site at Asn295.
authors.affiliation: School of Animal and Microbial Sciences, The University of Reading, Reading RG6 6AJ, UK.
authors.forename: Hongying
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bishop
authors.forename: Ian M
authors.initials: IM
authors.lastname: Jones
mesh_terms: D019138:Antibodies, Blocking / Q000235:genetics*; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000235:genetics* / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D000939:Epitopes; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins