title: Influence of co-administrated sinomenine on pharmacokinetic fate of paeoniflorin in unrestrained conscious rats.
abstract: Paeonia lactiflora Pall. (Ranunculaceae) root and Sinomenium acutum Rehder and Wilson (Menispermaceae) stem are two herbs widely used in Chinese medicine to treat rheumatoid arthritis. While, in theory, either herb could be used alone, in practice, Chinese medicine practitioners prescribe them together. Studies on pharmacokinetic interaction between the active constituents of these two herbs (paeoniflorin and sinomenine, respectively) provide empirical evidence to support their clinical practice. A single dose of paeoniflorin (150 mg/kg) alone and with sinomenine hydrochloride (90 mg/kg) was administered by gastric gavage to unrestrained conscious male Sprague-Dawley rats (n=5 or 6, 240-270 [corrected] g). Blood samples were collected periodically via a jugular vein before and after dosing from 10 min to 12 h. A high-performance liquid chromatographic (HPLC) assay was developed to determine the plasma concentrations of paeoniflorin. Non-compartmental pharmacokinetic profiles were constructed by using the software PK Solutions 2.0. The pharmacokinetic parameters were compared using unpaired Student t-test. After co-administration of sinomenine, the peak plasma concentration of paeoniflorin was elevated (P<0.01), the peak time was delayed (P<0.01), the AUC(0-t) was increased (P<0.001), the mean residence time (MRT) was prolonged (P<0.01), the C(L) was decreased (P<0.01) and the V(d) was reduced (P<0.05). These results indicate that sinomenine hydrochloride at 90 mg/kg significantly improved the bioavailability of paeoniflorin in rats.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong, PR China.
authors.forename: Zhong Qiu
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Zhi Hong
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Yuen Fan
authors.initials: YF
authors.lastname: Wong
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.forename: Xiong
authors.initials: X
authors.lastname: Cai
authors.forename: Hong Xi
authors.initials: HX
authors.lastname: Xu
authors.forename: Kelvin
authors.initials: K
authors.lastname: Chan
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001565:Benzoates / Q000097:blood / Q000493:pharmacokinetics*; D001682:Biological Availability; D001952:Bridged-Ring Compounds / Q000097:blood / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D039821:Monoterpenes; D009019:Morphinans / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D009019:Morphinans; C015423:peoniflorin; D006027:Glycosides; C009271:sinomenine