Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia.
Seventeen Streptococcus equi subsp. zooepidemicus strains isolated from camels and camel milk in Kenya and Somalia were identified by their cultural characteristics, by biochemical and serological reactions with the help of commercial identification systems and by molecular studies using a multiplex PCR. The isolates were further characterized by a PCR-mediated detection of size polymorphisms in the 16S-23S rDNA intergenic spacer region and the virulence gene szp and by amplification of the virulence gene cne. These molecular analysis are potentially useful in identifying and characterizing S. equi subsp. zooepidemicus strains of this origin and could possibly be valuable in epidemiological investigations.
Younan
M
M
Kenya Camel Association, PO Box 41607, 00100 Nairobi, Kenya.
Estoepangestie
A T S
AT
Cengiz
M
M
Alber
J
J
El-Sayed
A
A
Lämmler
C
C
D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D005798:Genes, Bacterial; D007630:Kenya; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012998:Somalia; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D018502:Streptococcus equi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014774:Virulence / Q000235:genetics
D021903:DNA, Ribosomal Spacer