[The immunological effects in guinea pig sensitized by trichloroethylene].
OBJECTIVE
To investigate the changes of certain immunological indexes in development of Guinea Pig allergic dermatitis induced by trichloroethylene (TCE).


METHODS
The Guinea Pig model of TCE-induced allergic dermatitis was established by Guinea Pig Maximisation Test. IgG levels in serum were measured by sandwich enzyme immunoassay. The splenic T lymphocytes ConA-stimulated proliferation function and natural killer (NK) cell activity were detected by MTT assay and micro-LDH release assay, respectively.


RESULTS
The sensitization rate of TCE was 66.7%. The IgG level in serum of TCE-sensitized animals was significantly higher than that in normal animals (P < 0.05). No obvious differences in Splenic T lymphocytes proliferation index and NK cell activity between TCE-sensitized and normal animals were found (P > 0.05).


CONCLUSIONS
Humoral immunity may play an important role in development of allergic dermatitis induced by TCE.
Dai
Yu-Fei
YF
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. yf_dai@sina.com
Nui
Yong
Y
Cheng
Juan
J
Leng
Shug-Uang
SU
D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood*; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D013601:T-Lymphocytes / Q000276:immunology; D014241:Trichloroethylene / Q000633:toxicity*
D007074:Immunoglobulin G; D014241:Trichloroethylene