Responses of sensitive and tolerant bush beans (Phaseolus vulgaris L.) to ozone in open-top chambers are influenced by phenotypic differences, morphological characteristics, and the chamber environment.
Responses of bush bean (Phaseolus vulgaris L.) lines 'S156' (O(3)-sensitive) and 'R123' (O(3)-tolerant), and cultivars 'BBL 290' (O(3)-sensitive) and 'BBL 274' (O(3)-tolerant) to ambient ozone (O(3)) were investigated during the 2001 and 2002 growing seasons. Seedlings were grown in pots inside open-top chambers (OTCs), with charcoal filtered (CF) and non-filtered (NF) ambient air, and in non-chambered ambient air (AA) plots. Growth parameters from individual plants were evaluated after harvests at the end of vegetative (V(4)) and reproductive (R(10)) growth phases. Results at V(4) indicated that CF did not provide additional benefits over NF in 'S156' in 2001 and 2002. In contrast, exposure to CF significantly impaired the growth of 'R123'. At the end of R(10), 'S156' produced more pods, most of which remained immature, and contained fewer seeds or were more frequently aborted, whereas pods produced in 'R123' reached pod maturation and senescence more consistently. Despite increased seed weights inside the OTCs, as observed in 'S156', differences between the two lines were insignificant when grown outside OTCs. Results from the 'BBL 290'/'BBL 274' pair, especially at V(4) phase, remained inconclusive. Plant morphological characteristics, variabilities in environmental conditions, and 'chamber effects' inside OTCs were influential in determining plant response to ambient O(3).
Elagöz
Vahram
V
Plant Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA. velagoz@nsm.umass.edu
Manning
William J
WJ
D000393:Air Pollutants / Q000633:toxicity*; D004777:Environment; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D027805:Phaseolus / Q000033:anatomy & histology / Q000187:drug effects* / Q000254:growth & development; D010641:Phenotype; D012639:Seeds
D000393:Air Pollutants; D010083:Oxidants, Photochemical; D010126:Ozone