Intracellular cAMP controls a physical association of V-1 with CapZ in cultured mammalian endocrine cells.
V-1, an ankyrin repeat protein with the activity to control tyrosine hydroxylase (TH) gene expression and transmitter release in PC12D cells, associates with CapZ, an actin capping protein, and thereby regulates actin polymerization in vitro. In this study, immunoprecipitation and Western blot analysis showed that V-1 was physically associated with CapZ-beta in PC12D transfectants overexpressing V-1. These proteins were co-localized in the soma of Purkinje cells of rat cerebellum as assayed by immunohistochemistry. Furthermore, in the V-1 transfectants, the amount of CapZ which physically associated with V-1 was steeply reduced at 2h after treatment with forskolin, but was thereafter increased to reach its initial level at 12h after forskolin-treatment. These results suggest that the association of V-1 with CapZ is controlled by a cAMP-dependent signalling pathway probably to play a functional role in the regulatory mechanism of actin dynamics in the endocrine system and the central nervous system.
Kitazawa
Masashi
M
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Yamakuni
Tohru
T
Song
Si-Young
SY
Kato
Chieko
C
Tsuchiya
Reiko
R
Ishida
Mami
M
Suzuki
Nobuhide
N
Adachi
Eijiro
E
Iwashita
Shintaro
S
Ueno
Susumu
S
Yanagihara
Nobuyuki
N
Taoka
Masato
M
Isobe
Toshiaki
T
Ohizumi
Yasushi
Y
D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D051347:CapZ Actin Capping Protein; D002460:Cell Line; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D000242:Cyclic AMP / Q000378:metabolism*; D004703:Endocrine System / Q000166:cytology / Q000378:metabolism*; D047468:Immunoprecipitation; D008840:Microfilament Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D011689:Purkinje Cells / Q000737:chemistry; D051381:Rats
D051347:CapZ Actin Capping Protein; D008840:Microfilament Proteins; D009124:Muscle Proteins; D000242:Cyclic AMP