title: Reference ranges of lymphocyte subsets of healthy adults in Turkey.
abstract: The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey.
  
  
  METHODS
  Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 +/- 16.69).
  
  
  RESULTS
  The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 +/- 8.44%, 1,680 +/- 528 cells/microl; CD4: 47.37 +/- 9.10%, 1,095 +/- 391 cells/microl; CD8: 28.99 +/- 5.99%, 669 +/- 239 cells/microl; CD19: 10.96 +/- 4.44%, 254 +/- 122 cells/microl and CD56: 7.03 +/- 3.26%, 161 +/- 92 cells/microl, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05).
  
  
  CONCLUSIONS
  Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Cukurova University, Balcali-Adana, Turkey. kyaman@cu.edu.tr
authors.forename: Akgun
authors.initials: A
authors.lastname: Yaman
authors.forename: Salih
authors.initials: S
authors.lastname: Cetiner
authors.forename: Filiz
authors.initials: F
authors.lastname: Kibar
authors.forename: Yeşim
authors.initials: Y
authors.lastname: Taşova
authors.forename: Gulşah
authors.initials: G
authors.lastname: Seydaoğlu
authors.forename: Ismail H
authors.initials: IH
authors.lastname: Dündar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D016516:CD4-CD8 Ratio; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014421:Turkey