In vitro detection and characterisation of a polyphosphate synthesising activity in the yeast Candida humicola G-1.
An in vitro detectable polyphosphate-synthesising activity was characterised using two independent assay systems in extracts of the yeast Candida humicola G-1. Its properties were similar to those of a range of bacterial polyphosphate kinase enzymes. PCR amplification of C. humicola genomic DNA using universal primers for bacterial polyphosphate kinase genes yielded a product whose translated sequence showed up to 34% amino acid similarity to the bacterial enzyme.
McGrath
John W
JW
School of Biology and Biochemistry and QUESTOR Centre, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Rd., Belfast, BT9 7BL, Northern Ireland. j.mcgrath@qub.ac.uk
Kulakova
Anna N
AN
Kulakov
Leonid A
LA
Quinn
John P
JP
D000595:Amino Acid Sequence; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000378:metabolism*; D016415:Sequence Alignment; D013696:Temperature
D003470:Culture Media; D011122:Polyphosphates; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase