Use of monoclonal antibodies in blocking ELISA detection of transmissible gastroenteritis virus in faeces of piglets.
Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive blocking ELISA format showed the highest sensitivity, allowing detection of 10(3) TCID50 TGEV/ml in culture medium. Ninety-nine porcine field faecal samples obtained from 37 herds affected with diarrhoea were examined, and various TGEV levels were found in nine samples from six herds. However, only in three samples were significant TGEV concentrations demonstrated. The relationship between incidence of TGEV gastroenteritis and the spread of porcine respiratory coronavirus infection in pig farms is discussed.
Rodák
L
L
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic. rodak@vri.cz
Smíd
B
B
Nevoránková
Z
Z
Valícek
L
L
Smítalová
R
R
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000821:virology; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis*; D051379:Mice; D019590:Nucleocapsid Proteins / Q000276:immunology; D011897:Random Allocation; D012680:Sensitivity and Specificity; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D019590:Nucleocapsid Proteins; D014763:Viral Matrix Proteins