Erythropoietin decreases cytotoxicity and nitric oxide formation induced by inflammatory stimuli in rat oligodendrocytes.
In the present study, we investigated whether erythropoietin (Epo) has a protective effect against cytotoxicity induced by interferon-gamma (IFN-gamma ) and lipopolysaccharide (LPS) in primary rat oligodendrocyte cultures. The possible modulatory effect of erythropoietin on inducible nitric oxide synthase (iNOS) mRNA expression and nitrite production were also analyzed. Erythropoietin exerted a significant protective effect against IFN-gamma and LPS-induced oligodendrocyte injury as determined by lactate dehydrogenase assay. Treatment with erythropoietin inhibited the expression of iNOS mRNA and nitrite production resulting from proinflammatory stimulation by IFN-gamma and LPS. These results suggest that erythropoietin has protective effects against inflammatory oligodendrocyte injury in vitro and may play a protective role in neurological disorders characterized by oligodendrocyte death, such as multiple sclerosis.
Genc
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Learning Resources Center Research Laboratory, School of Medicine, Dokuz Eylul University, Inciralti, 35340, Izmir, Turkey. kkursadgenc@hotmail.com
Genc
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Baskin
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Semin
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D000818:Animals; D000831:Animals, Newborn; D001921:Brain; D002470:Cell Survival; D002478:Cells, Cultured; D004921:Erythropoietin / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007371:Interferon-gamma; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D012333:RNA, Messenger; C433788:lipopolysaccharide, E. coli O26-B6; D004921:Erythropoietin; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat