[Growth, virulence and antigenecity of Leptospira interrogans serovar mozdok in modified EMJH medium].
The effect of higher Tween 80 concentrations in EMJH synthetic medium on the growth, virulence and antigenecity of Leptospira interrogans serovar mozdok was evaluated for increasing the performances and making a full use of the detoxifying capacity of bovine serum albumin. The growth was spectrophotographically evaluated by the analysis of the bacterial growth kinetics; the obtained biomass performance and the consumption of the carbon source. The virulence was estimated in Syrian Hamster model whereas antigenecity was determined through the microagglutination technique in rabbit's polyclonal antiserum. Under controlled culture conditions, the increase of Tween 80 concentration up to 3.25 mg/ml brought about an acceleration in bacterial metabolism that managed to double cell performances with a full consumption of the carbon source, without affecting virulence and antigenecity for a number of successive subcultures.
Rodríguez
Andrés Gonález
AG
Instituto Finlay, Centro de Investigación y Producción de Vacunas y Sueros, Ciudad de La Habana, Cuba. andresgles@finlay.edu.cu
Santiesteban
Niurka Batista
NB
Abreu
Yolanda Valdés
YV
González
González Marta
GM
D000818:Animals; D006224:Cricetinae; D003470:Culture Media; D007921:Leptospira interrogans / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*
D003470:Culture Media