title: Construction of an electrochemical DNA chip for simultaneous genotyping of single nucleotide polymorphisms.
abstract: An electrochemical DNA chip was constructed for simultaneous genotyping of single nucleotide polymorphisms (SNPs) using genomic DNA extracted from blood samples. This chip consisted of electrodes located on a single piece of substrate and allele-specific oligonucleotide probes on the electrodes. As a first application, the 4 SNPs (MxA[-88], MxA[-123], MBL[X/Y], and MBL[A/B]), which have association with the efficacy of interferon therapy for HCV patient, were genotyped on the new DNA chip. Following hybridization of PCR products containing the 4 types of fragments, washing, bisbenzimide H33258 (Hoechst 33258) reaction and electrochemical analyses, 59 blood samples were genotyped by the chip method simultaneously. All procedures were completed within 2 h and the results were 100% concordant with those by the direct sequence method. The electrochemical DNA chip is expected to be a practical tool for SNPs genotyping.
authors.affiliation: Toshiba Research & Development Center, 1, Komukai Toshiba-cho, Saiwai-ku, Kawasaki, Kanagawa Pref. 212-8582, Japan. masayoshi8.takahashi@toshiba.co.jp
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Jun
authors.initials: J
authors.lastname: Okada
authors.forename: Keiko
authors.initials: K
authors.lastname: Ito
authors.forename: Michie
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Koji
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Yuri
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Furuichi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ohta
authors.forename: Sunji
authors.initials: S
authors.lastname: Mishiro
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Gemma
mesh_terms: D006690:Bisbenzimidazole; D004247:DNA / Q000097:blood*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA; D006690:Bisbenzimidazole