Stir bar sorptive extraction: a new quantitative and comprehensive sampling technique for determination of chemical signal profiles from biological media.
Various applications of a novel sampling procedure in chemical ecology are outlined. The stir bar extraction method features the analytical reproducibility needed in recording the analytical profiles of volatile and semivolatile components of biological mixtures. This methodology has been demonstrated here through the examples of small volume urine samples, glandular tissue volatiles, and the air blown through animal cages. Its analytical merits are compared with those of the previously established purge-and-trap (dynamic headspace) technique.
Soini
Helena A
HA
Department of Chemistry, Institute for Pheromone Research Indiana University, Bloomington, Indiana 47405, USA.
Bruce
Kevin E
KE
Wiesler
Donald
D
David
Frank
F
Sandra
Pat
P
Novotny
Milos V
MV
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D019411:Clinical Laboratory Techniques; D006224:Cricetinae; D004463:Ecology; D005088:Exocrine Glands / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D051379:Mice; D010675:Pheromones / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012903:Smell / Q000502:physiology; D014556:Urine / Q000737:chemistry; D014835:Volatilization
D010675:Pheromones