title: Characterization of Shiga toxin-producing Escherichia coli isolated from aquatic environments.
abstract: This study reports the phenotypic and genotypic characterization of 144 Shiga toxin-producing Escherichia coli (STEC) strains isolated from urban sewage and animal wastewaters using a Shiga toxin 2 gene variant (stx(2))-specific DNA colony hybridization method. All the strains were classified as E. coli and belonged to 34 different serotypes, some of which had not been previously reported to carry the stx(2) genes (O8:H31, O89:H19, O166:H21 and O181:H20). Five stx(2) subtypes (stx(2), stx(2c), stx(2d), stx(2e) and stx(2g)) were detected. The stx(2), stx(2c), stx(2d) and stx(2e) subtypes were present in urban sewage and stx(2e) was the only stx(2) subtype found in pig wastewater samples. The stx(2c) and stx(2g) were more associated with cattle wastewater. One strain was positive for the intimin gene (eae) and five strains of serotypes were positive for the adhesin encoded by the saa gene. A total of 41 different seropathotypes were found. On the basis of occurrence of virulence genes, most non-O157 STEC strains are assumed to be low-virulence serotypes.
authors.affiliation: Departament de Microbiologia, Universitat de Barcelona, Diagonal 645, E-08028 Barcelona, Spain. crgarcia@ub.edu
authors.forename: C
authors.initials: C
authors.lastname: García-Aljaro
authors.forename: M
authors.initials: M
authors.lastname: Muniesa
authors.forename: J E
authors.initials: JE
authors.lastname: Blanco
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Jofre
authors.forename: A R
authors.initials: AR
authors.lastname: Blanch
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001434:Bacteriophage Typing; D002417:Cattle; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics; D005285:Fermentation; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D019081:O Antigens / Q000032:analysis; D012703:Serotyping; D012722:Sewage / Q000382:microbiology*; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000235:genetics*; D013012:Sorbitol / Q000378:metabolism; D013030:Spain; D013552:Swine; D037521:Virulence Factors / Q000235:genetics; D014871:Water Microbiology
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C022863:H antigen; D019081:O Antigens; C439650:SAA protein, E coli; D012722:Sewage; D022641:Shiga Toxin 2; D037521:Virulence Factors; C073819:eaeA protein, E coli; D013012:Sorbitol; D005966:Glucuronidase