Bone marrow donor routine HLA typing identified a novel B*07 allele, B*0734, confirmed by allele-specific DNA cycle sequencing.
In this article, we report the identification of a new human leukocyte antigen-B allele in a sample that was tested in our routine typing for bone marrow donors. This novel allele officially designed B*0734 was found in a female donor of Bavarian Caucasoid origin (Laboratory code 121036). The search for unrelated bone marrow donors was initiated by the Aktion Knochenmarkspende Bayern. In comparison to the common B*070201, B*0734 differs at four nucleotide positions, 412 (G-->A), 539 (G-->T), 559 (G-->A) and 560 (A-->C) causing three amino acid substitutions, at postion 138 Asp-->Asn, at position 180 Arg-->Leu and at position 187 Glu-->Thr.
Witter
K
K
Laboratory of Immunogenetics, University Munich, Paul-Heyse-Strasse 33, 80336 Munich, Germany. kwitter@kk-i.med.uni-muenchen.de
Volgger
A
A
Zahn
R
R
Scholz
S
S
Albert
E D
ED
D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D005260:Female; D005805:Genes, MHC Class I; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015793:HLA-B7 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014019:Tissue Donors; D044465:White People / Q000235:genetics
D015235:HLA-B Antigens; C503738:HLA-B*07:34 antigen; D015793:HLA-B7 Antigen