Osteoprogenitor viability in cell populations isolated from rat femora is not affected by 24 h storage at 4 degrees C.
This study was initiated to determine whether partially dissected bones of rats could be refrigerated for 24 h in saline without losing viability of progenitor cells, specifically osteoprogenitors. This is directly applicable to studies involving bone tissue requiring overnight shipment, for example, studies involving space flown animals, grafting experiments, or transplantation. We evaluated cell populations isolated from the proximal femur of 6-week-old male Fisher 344 rats. Explants from the left femur were prepared and placed into culture immediately following dissection, while the right femur was cleaned, fragmented, and stored in saline at 4 degrees C for 24 h, after which explant cultures were initiated. After 11 days of explant culture, cells were collected from outgrowths, counted, and plated to initiate experiments. Plated cells were grown for either 15 or 21 days. To determine if storage affected the total number of colony forming progenitors, alkaline phosphatase positive colonies, or the number of osteoprogenitors, were counted. There was no significant difference in any of the types of colony forming units examined between cell populations derived from freshly prepared samples or those stored for 24 h, indicating that storage at 4 degrees C of bone tissue for 24 h in saline does not affect the osteogenic potential or the number of osteoprogenitors of the cell populations isolated.
Basso
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N
Faculty of Dentistry, University of Toronto, Toronto, Ont., Canada M5G 1G6. nick.basso@utoronto.ca
Mirkopoulos
P
P
Heersche
J N M
JN
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D005269:Femur / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology
D000469:Alkaline Phosphatase