Simultaneous determination of anthraquinones, their 8-beta-D-glucosides, and sennosides of Rhei Rhizoma by capillary electrophoresis.
The simultaneous separation and determination of major anthraquinones (emodin, chrysophanol, rhein and their glucosides, aloe-emodin, sennoside A, and sennoside B) of Rhei Rhizoma were achieved by cyclodextrin modified capillary zone electrophoresis. The running electrolyte used in this method was 0.005 M alpha-cyclodextrin in 0.03 M borate buffer (pH 10.0) containing 20% acetonitrile, with an applied voltage of 20 kV.
Koyama
Junko
J
Kobe Pharmaceutical University, Kobe 658-8558, Japan. j-koyama@kobepharma-u.ac.jp
Morita
Izumi
I
Fujiyoshi
Hirotaka
H
Kobayashi
Norihiro
N
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification*; D012250:Rheum; D012676:Senna Extract; D000081226:Sennosides
D000880:Anthraquinones; D005960:Glucosides; D010936:Plant Extracts; D028321:Plant Preparations; D000081226:Sennosides; D012676:Senna Extract