title: Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo.
abstract: Nan-Chai-Hu, the root of Bupleurum scorzonerifolium, is a traditional Chinese herb used in treatment of liver diseases such as hepatitis and cirrhosis. We recently reported that the acetone extract of B. scorzonerifolium (BS-AE) could inhibit proliferation and induce apoptosis in A549 human lung cancer cells. We further examined its anti-proliferative mechanisms and in vivo anticancer effect. Our results showed that BS-AE had the ability to cause cell cycle arrest in G2/M phase, inducing tubulin polymerization, and activating caspase-3 and -9 in A549 cells. BS-AE-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk in majority. The result of in vivo study showed that BS-AE could suppress growth in A549 subcutaneous xenograft tumors. These results indicate that BS-AE exerts antiproliferative effects on A549 cells in vitro and in vivo, and prompted us to further evaluate and elucidate the chemical composition profile of BS-AE.
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan, ROC.
authors.forename: Yeung-Leung
authors.initials: YL
authors.lastname: Cheng
authors.forename: Shih-Chun
authors.initials: SC
authors.lastname: Lee
authors.forename: Shinn-Zong
authors.initials: SZ
authors.lastname: Lin
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Chang
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Chen
authors.forename: Nu-Man
authors.initials: NM
authors.lastname: Tsai
authors.forename: Yao-Chi
authors.initials: YC
authors.lastname: Liu
authors.forename: Ching
authors.initials: C
authors.lastname: Tzao
authors.forename: Dah-Shyong
authors.initials: DS
authors.lastname: Yu
authors.forename: Horng-Jyh
authors.initials: HJ
authors.lastname: Harn
mesh_terms: D000818:Animals; D017209:Apoptosis; D029972:Bupleurum / Q000737:chemistry; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: C051274:Bupleurum root extract; D010936:Plant Extracts