Optimisation and use of water-in-oil MEEKC in pharmaceutical analysis.
Water-in-oil microemulsion electrokinetic chromatography has been applied to the separation of a range of acids, bases and neutrals and is especially suitable for very water-insoluble drug compounds. A number of operating parameters were evaluated. An optimised set of operating conditions allowed separation of a range of pharmaceutical formulations containing water-insoluble compounds. A number of novel applications for W/O microemulsions were developed and ability to quantify drug contents in tablets and a cream was shown with good precision, detector linearity and accuracy. Comparison of obtained data with those determined from a HPLC method showed acceptable agreement.
Broderick
Margo
M
Waterford Institute of Technology, Department of Chemical and Life Sciences, Cork Road, Waterford, Ireland. mbroderick@wit.ie
Donegan
Sheila
S
Power
Joe
J
Altria
Kevin
K
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004563:Electrochemistry; D004655:Emulsions / Q000032:analysis* / Q000737:chemistry; D009821:Oils / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000032:analysis / Q000737:chemistry
D004655:Emulsions; D009821:Oils; D014867:Water