T-cell specific enhancement of histone H3 acetylation in 5' flanking region of the IL-2 gene.
The IL-2 gene expression is highly T-cell specific in an activation-dependent manner. However, little is known about how T-cell specific expression is regulated, although related transcription factors have been identified. To address this issue, we examined the chromatin structure change of the IL-2 gene by analyzing histone acetylation status in the upstream of IL-2 gene transcription initiation site. Interestingly, the histone acetylation level was found to be higher in various sites on the widespread upstream region in resting T-cells than resting B-cells. After T-cell stimulation with PMA and ionomycin, the same regions were further acetylated on histone H3. Particularly, the distal enhancer region showed prompt enhancement of histone acetylation, followed by the IL-2 mRNA expression. These results suggest that the 5' flanking region including the distal enhancer region of the IL-2 gene is already accessible in T cells with constitutive acetylation of histone H3,which may serve for T-cell specific IL-2 expression.
Wang
Lili
L
Department of Immunology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Kametani
Yoshie
Y
Katano
Ikumi
I
Habu
Sonoko
S
D024506:5' Flanking Region / Q000235:genetics*; D000107:Acetylation; D000818:Animals; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D007376:Interleukin-2 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*; D024363:Transcription Initiation Site
D006657:Histones; D007376:Interleukin-2; D003850:Deoxyribonuclease I