The gene yggE functions in restoring physiological defects of Escherichia coli cultivated under oxidative stress conditions.
DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 vector. The content of reactive oxygen species (ROS) in IM303 (I4) cells carrying pYGE was reduced to 31% of ROS content in the control cells with pUC 19. In the culture of wild-type strain, E. coli MM294, in the medium with paraquat (10 micromol/l), maximum specific growth rate of the cells with pYGE was about five times higher than that of the control cells, with a decreased ROS content in the former cells. The introduction of pYGE also suppressed the occurrence of the cells with altered amino acid requirement in the culture of MM294 cells with paraquat.
Kim
Sun Young
SY
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
Nishioka
Motomu
M
Hayashi
Shuhei
S
Honda
Hiroyuki
H
Kobayashi
Takeshi
T
Taya
Masahito
M
D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000502:physiology; D005798:Genes, Bacterial / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000172:deficiency / Q000502:physiology
D029968:Escherichia coli Proteins; D013482:Superoxide Dismutase