The effects of Chinese herb Angelica in focal cerebral ischemia injury in the rat.
This experiment was designed to study the therapeutic mechanisms of Angelica on the focal cerebral ischemia injury of the rat. The ischemic area was determined by TTC stain. And terminal deoxynucleotidyl transferase (TDT) mediated DUTP-biotin nick end labeling (TUNEL) method was applied to detect neuronal apoptosis. The expressions of Bcl-2 and Bax proteins were observed by immunohistochemical staining methods. Results show that the treatment with angelica reduced the volume of cerebral infarction (p < 0.05), and that the number of neuronal apoptosis cells decreased significantly (p < 0.01). Also the expression level of Bax protein decreased (p < 0.01). These results suggest that Angelica can reduce the number of apoptosis cells by decreasing the expression of Bax protein. This is maybe one of the mechanisms of the therapeutic effect of Angelica on focal cerebral ischemia injury.
Yang
Jing Wei
JW
Department of Pathophysiology, Medical College of Wuhan University, Wuhan 430071, China.
Ouyang
Jing Ping
JP
Liao
Wei Jing
WJ
Tian
Jun
J
Liu
Yong Ming
YM
Wei
Lei
L
Wang
Bao Hua
BH
Li
Ke
K
D029969:Angelica / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy*; D004396:Coloring Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007511:Ischemia; D009474:Neurons / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods; D018514:Plant Structures / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts / Q000494:pharmacology; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D013778:Tetrazolium Salts; D051028:bcl-2-Associated X Protein; C009591:triphenyltetrazolium