[Neutrophil oxidative metabolism in children with chronic hepatitis C].
A growing number of data suggest the role of reactive oxygen species (ROS) in the pathogenesis of chronic type c hepatitis. Activated neutrophils, macrophages and Kupffer cells are basic sources of ROS in the course of the inflammatory process in the liver. The aim of the study was to assess the metabolism of neutrophil oxygen in children with chronic hepatitis C.


METHODS
The study comprised 14 children with chronic hepatitis C (group 1), 4 children with positive serum anti-HCV antibodies and negative HCV RNA as well as normal ALT activity (group II) and 6 healthy children 9 (group III). Production of reactive oxygen species by neutrophils using the method of chemiluminescence (CL) with luminol (MLX Microtitier Plate Luminometr, Dynex, USA) was assessed. We estimated chemiluminescence response (CL max and CL total) of neutrophiles both unstimulated and stimulated by formyl-methionyl-leucyl-phenyloalanine (fMLP), opsonized zymosan (OZ), phorbol-myristate-acetate (PMA) without and after priming with tumor necrosis factor alpha (TNF-?) was estimated.


RESULTS
CL max f MLP stimulated without preactivation was significantly lower in group I and II than in group III (p<0.04, p<0.06, respectively). CL max OZ stimulated was significantly higher in group I in comparison to group II (p<0.04) and it tended to be higher in group I than in group III. CL max PMA stimulated was lower in group I compared to group III (p<0.08). CL total fMLP stimulated was lower in group II (p<0.07) compared to group I and it tended to be lower in group I and II compared to group III. CL total OZ stimulated was significantly higher in group I than in group II (p<0.05) and group III (p<0.03). CL max and CL total OZ stimulated and preactivated with TNFalpha was significantly lower in group I compared to group III (p<0.02 and p<0.04, respectively).
In the course of chronic hepatitis C in children, both neutrophil preactivation and inhibition of neutrophil release of reactive oxygen species, which occur as a result of different mechanisms, are observed.
Wiśniewska-Ligier
Małgorzata
M
III Klinika Pediatrii Instytutu "Centrum Zdrowia Matki Polki" w Lodzi.
Woźniakowska-Gesicka
Teresa
T
Lewkowicz
Przemysław
P
Kupś
Joanna
J
Andrzejewski
Arkadiusz
A
D000293:Adolescent; D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000276:immunology / Q000503:physiopathology*; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000914:Antibodies, Viral; D017382:Reactive Oxygen Species