The Salmonella translocated effector SopA is targeted to the mitochondria of infected cells.
This study investigates the Salmonella effector protein SopA. We show that in Salmonella enterica serovar Dublin-infected cells, SopA(1-347) fused to two carboxy-terminal hemagglutinin tags partially colocalized with mitochondria. Transfection of eukaryotic cells with a panel of constructs encoding truncated versions of SopA identified that amino acids 100 to 347 were sufficient to target SopA to the mitochondria.
Layton
Abigail N
AN
Institute for Animal Health, Compton, Berkshire, United Kingdom.
Brown
Philip J
PJ
Galyov
Edouard E
EE
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D012480:Salmonella Infections / Q000378:metabolism* / Q000382:microbiology; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D013347:Subcellular Fractions; D014774:Virulence
D001426:Bacterial Proteins; C060971:SopA protein, Bacteria