Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples.
Biochemical and pharmacological properties of biosurfactants produced at 45 degrees C temperature by Pseudomonas aeruginosa mucoid (M) and non-mucoid (NM) strains, isolated from hydrocarbon-contaminated soil samples, were characterized. Both the strains secreted appreciable amount of biosurfactants (5.0-6.5 g/l), responsible for the reduction of surface tension of the medium from 68 to 29 +/- 0.5 mN/m post 96 h of growth. Maximum yield of biosurfactants was observed following the supplementation of NH(4)Cl and glycerol as nitrogenous source and carbon source, respectively. These thermostable biosurfactants exhibited strong emulsifying property and could release appreciable amount of oil from saturated sand-pack column. Pharmacological characterization of these biosurfactants revealed that they induced dose-dependent hemolysis and coagulation of platelet-poor plasma but were non-detrimental to chicken lung, liver, heart and kidney tissues. Our study has documented that biosurfactants from P. aeruginosa M and NM strains could be exploited for use in petroleum sectors as well in pharmaceutical industries.
Das
Kishore
K
Department of Molecular Biology and Biotechnology, ONGC Center for Petroleum Biotechnology, Tezpur University, India.
Mukherjee
Ashis K
AK
D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D006838:Hydrocarbons; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development; D012988:Soil Microbiology; D012989:Soil Pollutants; D013045:Species Specificity; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D000890:Anti-Infective Agents; D006838:Hydrocarbons; D011135:Polysaccharides, Bacterial; D012989:Soil Pollutants; D013501:Surface-Active Agents