Molecular characterization of the first satellite DNA with CENP-B and CDEIII motifs in the bat Pipistrellus kuhli.
The centromere is an essential structure in the chromosomes of all eukariotes and is central to the mechanism that ensures proper segregation during mitosis and meiosis. The comparison of DNA sequence motifs, organization and kinetocore components from yeast to man is beginning to indicate that, although centromeres are highly variable DNA elements, a conserved pattern of sequence arrangement and function is emerging. We have identified and characterized the first satellite DNA (P.k.SAT) from microbat species Pipistrellus kuhli. The presence of mammalian CENP-B box and yeast CDEIII box could indicate the participation of P.k.SAT in centromere organization.
Fantaccione
Stefania
S
Department of Life Science, II University of Naples, Caserta, Italy.
Pontecorvo
Giovanni
G
Zampella
Vincenzo
V
D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001483:Base Sequence; D015139:Blotting, Southern; D051936:Centromere Protein B; D002685:Chiroptera / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D015825:Chromosomes, Fungal / Q000235:genetics; D003001:Cloning, Molecular; D019175:DNA Methylation; D004276:DNA, Satellite / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016678:Genome; D023281:Genomics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013045:Species Specificity
D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins