Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis.
Using cDNA microarray hybridization from a human testicular cDNA library, one gene named lactate dehydrogenase A-like gene (LDHL, also known as LDHL6B) was cloned. LDHL exhibited 3.8-fold difference at expression level between adult and fetal human testes. The full cDNA length of LDHL is 1680 bp and had a 1145 bp open reading frame, which encoded a 41.9 kDa protein of 381 amino acids. Sequence analysis showed that LDHL harbors all the domains (one lactate/malate dehydrogenase, NAD binding domain and one lactate/malate dehydrogenase, alpha/beta C-terminal domain) in lactate dehydrogenase gene family. Blasting human genome database localized LDHL to human chromosome 15q22.2 and it was an intronless gene. Results of multiple-tissue PCR and real-time PCR showed that LDHL expressed mainly in testis and its mRNA abundance was testis development-related. In summary, LDHL is believed to be involved in testis development and spermatogenesis.
Wang
Hui
H
Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, PR China.
Zhou
Zuomin
Z
Lu
Li
L
Xu
Zhiyang
Z
Sha
Jiahao
J
D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007438:Introns; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012708:Sertoli Cells / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*
D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase