Effects of SDS, PBS solutions upon fluorescence values of Dermatophagoides pterronyssinus radioallergosorbent test inhibition.
OBJECTIVE
To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition.


METHODS
Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12505 and 24776.


RESULTS
Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively.


CONCLUSIONS
SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.
Sun
Jin-lü
JL
Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730. sunjl5@yahoo.com
Zhang
Hong-yu
HY
He
Hai-juan
HJ
Wang
Rui-qi
RQ
D000485:Allergens; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D010710:Phosphates / Q000008:administration & dosage* / Q000494:pharmacology; D011852:Radioallergosorbent Test / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage* / Q000494:pharmacology; D012996:Solutions; D013501:Surface-Active Agents / Q000008:administration & dosage* / Q000494:pharmacology
D000485:Allergens; D010710:Phosphates; D012996:Solutions; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D007073:Immunoglobulin E