[Study of genes that differentially expressed in eosinophils of patients with asthma by suppression subtractive hybridization technology].
OBJECTIVE
To study genes that differentially expressed in peripheral blood eosinophils of patient with asthmatic.


METHODS
Total RNA extracted from eosinophils of patient at the time of exacerbation was taken as the tester and the total RNA from eosinophils of the same patient after improvement as driver. The ds cDNA was synthesized by SMART PCR cDNA Synthesis technology. The differentially expressed genes were obtained by suppression subtractive hybridization (SSH) technology and were further identified by Southern blot.


RESULTS
Twelve differentially expressed genes including transformation growth factor beta activated kinase like (TAK1L) protein, cGMP gated channel protein were obtained.


CONCLUSIONS
Increased expression of these genes were involved in the regulation of proinflammatory response, signal transduction, energy metabolism and cell apoptosis, which may help to clarify the molecular mechanism of eosinophils in asthma and may provide the theoretical base for finding out the new medicines towards eosinophils.
Xu
Jin-song
JS
Department of Respiratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Cai
Shao-xi
SX
Zou
Fei
F
Tong
Wan-cheng
WC
Zhao
Hai-jin
HJ
D001249:Asthma / Q000097:blood / Q000235:genetics* / Q000473:pathology; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D015398:Signal Transduction
D018076:DNA, Complementary