Characterization of gp41 gene of Spodoptera litura multicapsid nucleopolyhedrovirus.
Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) gp41 gene is 993 bp long and the protein encoded by this gene has 6-66% amino acid identities with other known baculovirus GP41 proteins. Slgp41 transcripts were detected from 12 to 96 h post-infection (p.i.) and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infected S. litura cells detected a 41 kDa protein, and this protein was present in the nucleus of infected cells from 12 to 96 h p.i., whereas in the cytoplasm from 24 to 96 h p.i. Structural localization confirmed that SlGP41 is associated with the envelope of occlusion-derived virus (ODV). Lectin-binding assay showed that three lectins erythrina cristaglli lectin (ECL), lycopersicon esculentum lectin (LEL), and bandeiraea simlicifolia lectin (BSL) recognizing N-acetylglucosamine were specifically bound to SlGP41. It was proposed that SlGP41 is an O-glycoprotein.
Pan
Lijing
L
State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, People's Republic of China.
Li
Zhaofei
Z
Gong
Yingxue
Y
Yu
Mei
M
Yang
Kai
K
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D005944:Glucosamine / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D037121:Plant Lectins / Q000378:metabolism; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D018411:Spodoptera / Q000821:virology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C045629:erythrina lectin; C094173:nuclear polyhedrosis virus gp41; C034981:tomato lectin; D005944:Glucosamine