Control of DNA hybridization with photocleavable adducts.
Previous reports have shown that 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester (DMNPE) adducts coupled to DNA plasmids block transcription in vitro and in vivo until removed with light. In this report, we explore the use of DMNPE to control DNA hybridization. We found that DMNPE-caged oligonucleotides have changed spectrophotometric and electrophoretic properties that can be restored with light exposure. Caged oligonucleotides have slower electrophoretic mobility than noncaged oligonucleotides and caged oligonucleotides exposed to light. Effects of caging on hybridization were assessed in a fluorescence-based assay using a 20mer caged DNA oligonucleotide complementary to a 30mer molecular beacon. Fluorescence results indicate that hybridization is reduced and subsequently restored by light. Subsequent gel shift assays confirmed these results. Hybridization activity of caged oligonucleotides with an average of 14-16 DMNPE adducts per oligonucleotide was 14% of noncaged control oligonucleotides and after 365 nm photolysis, increased to nearly 80% of controls. Spectrophotometric characterization of caged oligonucleotides exposed to light and then filtered to remove the released DMNPE adducts indicates two to four attached cage groups remaining following photoactivation. These results suggest that this light-based technology can be used as a tool for the spatial and temporal regulation of hybridization-based DNA bioactivity.
Ghosn
Bilal
B
Department of Biological & Agricultural Engineering, Louisiana State University and LSU AgCenter, Baton Rouge, LA 70803, USA.
Haselton
Frederick R
FR
Gee
Kyle R
KR
Monroe
W Todd
WT
D004247:DNA / Q000528:radiation effects*; D009578:Nitrobenzenes; D009693:Nucleic Acid Hybridization / Q000528:radiation effects*; D010957:Plasmids
D009578:Nitrobenzenes; C085061:1-(4,5-dimethoxy-2-nitrophenyl)diazoethane; D004247:DNA