Urinary excretion of epsilondA is not predictive of cancer development: a prospective nested case-control study.
Human biomonitoring of the lipid peroxidation DNA modification 1,N6-ethenodeoxyadenosine (epsilondA) excreted into urine is thought to be a potential marker for oxidative stress-related DNA damage and human cancer. We have tested this hypothesis in a prospective, nested case-control study. During the years 1984-1989, 24-h urines were collected from 1956 men in the Kuopio Ischaemic Heart Disease (KIHD) Risk Factor Study. epsilondA concentrations were measured by LC-MS/MS in 24-h urine samples from 47 men with cancer diagnosed at follow up until 2001 and from 31 cancer free smoking-matched control subjects. Odds ratio for having higher than control median epsilon dA excretion rate and cancer, estimated by binary logistic regression, was 0.73 (95% CI 0.29-1.80, p = 0.49). In this study, the urinary excretion of epsilondA provides no additional prediction of cancer development in males after controlling for smoking.
Hillestrøm
Peter R
PR
Department of Clinical Pharmacology Q7642, Rigshospitalet, University Hospital Copenhagen, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
Nyssönen
Kristiina
K
Tuomainen
Tomi-Pekka
TP
Pukkala
Eero
E
Salonen
Jukka T
JT
Poulsen
Henrik E
HE
D000328:Adult; D015992:Body Mass Index; D001835:Body Weight; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts; D004249:DNA Damage; D003839:Deoxyadenosines / Q000652:urine*; D006801:Humans; D015227:Lipid Peroxidation; D016015:Logistic Models; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis* / Q000652:urine; D016017:Odds Ratio; D018384:Oxidative Stress; D011446:Prospective Studies; D012306:Risk; D012307:Risk Factors; D012907:Smoking; D013997:Time Factors
D018736:DNA Adducts; D003839:Deoxyadenosines; C032221:1,N(6)-ethenodeoxyadenosine; D004247:DNA