title: Automated system for release studies of salicylic acid based on a SIA method.
abstract: The aim of this work was to describe a fully automated system for the in vitro release testing of semisolid dosage forms based on SIA technique. The system was tested for monitoring release profiles of different ointments containing 3% of salicylic acid (Belosalic, Diprosalic, Triamcinolone S). The native fluorescence of salicylic acid was used for fluorimetric detection. Phosphate buffer pH 7.4 was the receptor medium; samples were taken at 10 min intervals during 6 h of the release test; and each test was followed by calibration with five standard solutions. The linear calibration range was 0.05-10 microg ml(-1) (r = 0.9996, six standards); the maximal SIA sample throughput for this system was 120 h(-1), sample volume being 50 microl and flow rate 50 microl s(-1). The detection limit for salicylic acid was 0.01 microg ml(-1).
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové 50005, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Klimundová
authors.forename: H
authors.initials: H
authors.lastname: Sklenárová
authors.forename: U F
authors.initials: UF
authors.lastname: Schaefer
authors.forename: P
authors.initials: P
authors.lastname: Solich
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D020156:Salicylic Acid / Q000032:analysis* / Q000493:pharmacokinetics*
chemical_list: D020156:Salicylic Acid