title: [Effect of sulphamethazine on the gene expression of FRTL-5 cells].
abstract: OBJECTIVE
  To study the effect of Sulphamethazine on the gene expression of FRTL-5 cells, and to explore the mechaniams of environmental thyroid hormone disruptors.
  
  
  METHODS
  cDNA microarray technique was used to analyze the gene expression of FRTL-5 cells of exponential phase treated by 2.0 microg/ml Sulphamethazine for 24 h. Total RNA from treated and untreated cells were labeled by Cy3 dCTP and Cy5 dCTP respectively. The ratios of Cy3/Cy5 were calculated in order to find the genes which expressed differently.
  
  
  RESULTS
  There were 679 genes (approximately 7%, total 9753 genes) exhibiting different expression, in which 395 (4.0%) genes up-regulated and 284 (3.0%) genes down-regulated. These genes related to regulation of gene expression, regulation of cell cycle, metabolism in cells, and so on.
  
  
  CONCLUSIONS
  The effect of Sulphamethazine on FRTL-5 cells may be related with a series of genes.
authors.affiliation: West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Wang
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Pan
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D052244:Endocrine Disruptors / Q000633:toxicity*; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000187:drug effects* / Q000235:genetics; D013418:Sulfamethazine / Q000633:toxicity*; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D011988:Receptors, Thyroid Hormone; D013418:Sulfamethazine