An amperometric glucose biosensor based on glucose oxidase immobilized in electropolymerized poly(o-aminophenol) and carbon nanotubes composite film on a gold electrode.
An amperometric glucose biosensor is developed that is based on immobilization of glucose oxidase (GOD) in a composite film of poly(o-aminophenol) (POAP) and carbon nanotubes (CNT), which are electrochemically co-polymerized at a gold (Au) electrode. Because of the high surface per volume ratio and excellent electrical conductivity of CNT, the biosensor based on an Au/POAP/CNT/GOD electrode has lower detection limit (0.01 mM), larger maximum response current (0.24 mA cm(-2)) and higher sensitivity (11.4 mA M(-1) cm(-2)) than the values of the biosensor based on an Au/POAP/GOD electrode. Additionally, the biosensor shows fast response time, large response current, and good anti-interferent ability for ascorbic acid, uric acid and acetaminophen. Good reproducibility and stability of the biosensor are also observed.
Pan
Dawei
D
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Chen
Jinhua
J
Yao
Shouzhuo
S
Tao
Wenyan
W
Nie
Lihua
L
D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D004566:Electrodes; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold; D007202:Indicators and Reagents; D043942:Nanotubes; D011108:Polymers / Q000737:chemistry*; D011199:Potentiometry
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D011108:Polymers; C505204:poly(2-aminophenol); D002244:Carbon; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose