Isothermal RNA sequence amplification method for rapid antituberculosis drug susceptibility testing of Mycobacterium tuberculosis.
RNA transcript quantification by an isothermal sequence amplification reaction was evaluated for susceptibility testing of 15 Mycobacterium tuberculosis strains. Agreement with the proportion method on Ogawa egg medium and the BACTEC MGIT 960 system was 100 and 87% for rifampin, 93 and 100% for isoniazid, 60 and 53% for ethambutol, and 80 and 80% for streptomycin, respectively.
Takakura
Shunji
S
Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Kyoto, 606-8507, Japan. stakakr@kuhp.kyoto-u.ac.jp
Tsuchiya
Shigeo
S
Fujihara
Naoko
N
Kudo
Toyoichiro
T
Iinuma
Yoshitsugu
Y
Mitarai
Satoshi
S
Ichiyama
Satoshi
S
Yasukawa
Kiyoshi
K
Ishiguro
Takahiko
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012329:RNA, Bacterial / Q000187:drug effects*; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D003470:Culture Media; D012329:RNA, Bacterial