Increased expression of L-type high voltage-gated calcium channel alpha1 and alpha2/delta subunits in mouse brain after chronic nicotine administration.
We investigated the effect of chronic nicotine administration on high voltage-gated calcium channels (HVCCs) in the mouse cerebral cortex. The treatment significantly increased expression of alpha1C, alpha1D, alpha1F, and alpha2/delta1 subunits with no changes of beta4 subunit of L-type HVCCs. [(3)H]Diltiazem binding to the particulate fractions increased with increased Bmax value. These results indicate that chronic nicotine treatment up-regulates L-type HVCCs, which is due to increased expression of alpha1 and alpha2/delta1 subunits.
Hayashida
Shinsuke
S
Department of Pharmacology, Kawasaki Medical School, Matsushima, Kurashiki 701-0192, Japan.
Katsura
Masashi
M
Torigoe
Fumiko
F
Tsujimura
Atsushi
A
Ohkuma
Seitaro
S
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002121:Calcium Channel Blockers / Q000493:pharmacokinetics; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects*; D004110:Diltiazem / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004347:Drug Interactions; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D008464:Mecamylamine / Q000494:pharmacology; D051379:Mice; D009538:Nicotine / Q000008:administration & dosage*; D018722:Nicotinic Agonists / Q000008:administration & dosage*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D014316:Tritium / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D018722:Nicotinic Agonists; D018733:Nicotinic Antagonists; D021122:Protein Subunits; D014316:Tritium; D008464:Mecamylamine; D009538:Nicotine; D004110:Diltiazem