title: Plasmid vectors for protein production, gene expression and molecular manipulations in Aspergillus niger.
abstract: We constructed three sets of plasmids for use in Aspergillus niger. These plasmids were assembled using various combinations of a series of modular DNA cassettes that included a selectable marker, pyrG, derived from Aspergillus nidulans; two promoter regions for directing protein expression; a cassette derived from the AMA1 replicator sequence to support autonomous replication; and a reporter gene based on the A. niger lacA gene. One set included integrating and autonomously replicating plasmids for the expression of homologous and heterologous proteins. The second was a set of autonomously replicating plasmids, with a secreted beta-galactosidase encoding reporter gene, for studying gene regulation events. The third set included pyrG-derived gene-blaster cassettes suitable for genome manipulation by targeted gene replacement.
authors.affiliation: Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Que., Canada H4B 1R6. storms@vax2.concordia.ca
authors.forename: Reginald
authors.initials: R
authors.lastname: Storms
authors.forename: Yun
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hongshan
authors.initials: H
authors.lastname: Li
authors.forename: Susan
authors.initials: S
authors.lastname: Sillaots
authors.forename: Amalia
authors.initials: A
authors.lastname: Martinez-Perez
authors.forename: Adrian
authors.initials: A
authors.lastname: Tsang
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000235:genetics*; D004261:DNA Replication; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017930:Genes, Reporter; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D011506:Proteins; D001616:beta-Galactosidase