title: Trapping, deformation, and rotation of giant unilamellar vesicles in octode dielectrophoretic field cages.
abstract: The behavior of freestanding lipid bilayer membranes under the influence of dielectric force potentials was studied by trapping, holding, and rotating individual giant unilamellar vesicles (GUVs) inside dielectrophoretic microfield cages. Using laser scanning confocal microscopy and three-dimensional image reconstructions of GUVs labeled with fluorescent membrane probes, field strength and frequency-dependent vesicle deformations were observed which are explained by calculations of the dielectric force potentials inside the cage. Dynamical membrane properties under the influence of the field cage were studied by fluorescence correlation spectroscopy, circumventing potential artifacts associated with measurements involving GUV immobilization on support surfaces. Lipid transport could be accelerated markedly by the applied fields, aided by hydrodynamic fluid streaming which was also studied by fluorescence correlation spectroscopy.
authors.affiliation: School of Applied & Engineering Physics, Clark Hall, Cornell University, Ithaca, New York, USA.
authors.forename: J
authors.initials: J
authors.lastname: Korlach
authors.forename: C
authors.initials: C
authors.lastname: Reichle
authors.forename: T
authors.initials: T
authors.lastname: Müller
authors.forename: T
authors.initials: T
authors.lastname: Schnelle
authors.forename: W W
authors.initials: WW
authors.lastname: Webb
mesh_terms: D001703:Biophysics / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D004058:Diffusion; D007834:Lasers; D008051:Lipid Bilayers / Q000737:chemistry*; D050356:Lipid Metabolism; D008081:Liposomes / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods*; D015233:Models, Statistical; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D014867:Water