title: Sandwich immunoassays for the determination of peanut and hazelnut traces in foods.
abstract: People suffering from food allergies are dependent on accurate food labeling, as an avoidance diet is the only effective countermeasure. Even a small amount of allergenic protein can trigger severe reactions in highly sensitized patients. Therefore, sensitive and reliable tests are needed to detect potential cross-contamination. In this paper two fast sandwich immunoassays are described for the determination of peanut (Arachis hypogaea) and hazelnut (Corylus avellana) traces in complex food matrices. Mouse monoclonal antibodies were used as capture antibodies, and labeled rabbit polyclonal antibodies were used as detection antibodies in both assays. The assay time was 30 min in total, and cross-reactivities against a variety of fruits and seeds were found to be in the low 10(-4)% (ppm) level or in some cases not detectable. The recoveries in all tested food matrices ranged from 86 to 127%, and the limits of detection were in the range of 0.2-1.2 mg/kg (ppm) in food for both peanut and hazelnut, respectively.
authors.affiliation: Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kiening
authors.forename: Reinhard
authors.initials: R
authors.lastname: Niessner
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Drs
authors.forename: Sabine
authors.initials: S
authors.lastname: Baumgartner
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.forename: Monique
authors.initials: M
authors.lastname: Bremer
authors.forename: Victoria
authors.initials: V
authors.lastname: Tomkies
authors.forename: Paul
authors.initials: P
authors.lastname: Reece
authors.forename: Chris
authors.initials: C
authors.lastname: Danks
authors.forename: Ulrike
authors.initials: U
authors.lastname: Immer
authors.forename: Michael G
authors.initials: MG
authors.lastname: Weller
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D010367:Arachis / Q000737:chemistry / Q000276:immunology*; D031211:Corylus / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D051379:Mice; D010940:Plant Proteins / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D010940:Plant Proteins