Characterization of quaternary ammonium oligomers by paired-ion reversed-phase liquid chromatography-mass spectrometry.
The separation of novel permanently charged oligomers was studied using paired-ion reversed-phase liquid chromatography. The polyionene studied is less than 5 kDa in size, but contains three oligomer series with different end-group chemistries. The complexity of this polyionene makes development of a single-dimension separation quite challenging. Separation under critical conditions was employed to fractionate the end-group conformations and then the chain length of the oligomers in each series was confirmed by LC-MS. The oligomers were then used to optimize a single-dimension HPLC separation. Precise modulation of the hydrophobicity of the ion-pair reagent and the stationary-phase chemistry yielded very high resolution one-dimensional separations.
Bourque
A J
AJ
Analytical Research and Development, Genzyme Drug Discovery and Development, Genzyme Corporation, 153 Second Avenue, Waltham, Massachusetts 02451, USA.
Clark
S P
SP
Kloss
A
A
Wang
B H
BH
Cohen
A S
AS
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D011108:Polymers / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
D011108:Polymers; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds