Escherichia coli O157:H7 detection limit of millimeter-sized PZT cantilever sensors is 700 cells/mL.
A composite self-excited millimeter-sized lead zirconate titanate (PZT) glass cantilever (2 mm x 1.8 mm; sensing area of 6 mm2) was fabricated for the detection of Escherichia coli (E. coli) O157:H7. The fundamental and second mode resonance in air was 10.95 +/- 0.05 kHz and 43.45 +/- 0.05 kHz, respectively. Affinity purified monoclonal antibody (anti-E. coli O157:H7) specific to the pathogen E. coli O157:H7 was immobilized at the cantilever glass tip, and then immersed in liquid containing the pathogen (70 to 7 x 10(7) cells/mL). The resonant frequency showed a reduction and reached a steady state shift of 0 +/- 5, 46 +/- 5, 260 +/- 5, and 1010 +/- 5 Hz corresponding to 0, 700, 7000, and 7 x 10(7) cells/mL. From the experiments conducted, the detection limit of the sensor was 700 cells/mL.
Campbell
Gossett A
GA
Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USA.
Mutharasan
Raj
R
D000465:Algorithms; D000907:Antibodies, Bacterial / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D004566:Electrodes; D019453:Escherichia coli O157 / Q000737:chemistry*; D007202:Indicators and Reagents; D007854:Lead / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; C065536:lead titanate zirconate; D007854:Lead; D015040:Zirconium; D014025:Titanium