Cyclic stretch induced MAPK phosphorylation stronger than static stretch.
The environment for living organism in space has microgravity and/or hypergravity and/or any kind of mechanical stresses. Cellular response may differ from the variety of mechanical stress. Mitogen-activated protein kinases (MAPKs) pathway is related to various cellular events. In the present study it was investigated the serial measurement of MAPK phosphorylation using western-blotting analysis following with three types of cyclic stretch, static, 0.1 Hz and 0.25 Hz. The result was that induction of MAPK phosphorylation had peaks within 2 to 4 hours and attenuated, while induction of p38 phosphorylation in 0.1 Hz stretch had a peak at 6 hours later and the strongest. Thus, there might be differential cellular response depends upon the frequency of cyclic stretch .
Kanazawa
Yuichiro
Y
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Chiba Univ.
Nomura
Jun
J
Himeta
Juni
J
Suzuki
Toshikazu
T
Suzuki
Nobuo
N
Yoshimoto
Shinya
S
Ichinose
Masaharu
M
D015153:Blotting, Western; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D010766:Phosphorylation; D015398:Signal Transduction / Q000502:physiology*; D013314:Stress, Mechanical; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D048051:p38 Mitogen-Activated Protein Kinases