Quantitative analysis of the marker compounds in Salvia miltiorrihiza root and its phytomedicinal preparations.
A HPLC method is described in order to separate and identify 4 phenolic compounds including lithospermic acid B, 3,4-dihydroxyphenyllactic acid (danshensu), rosmarinic acid, and protocatechuic aldehyde as well as 3 lipophilic ones (tanshinone I, tanshinone II(A) and cryptotanshinone) from the roots of Salvia miltiorrihiza or the herbal product containing S. miltiorrihiza available in Chinese or Japanese market. The influence of extractive conditions, such as method, solvents and time, on the 4 phenolic marker components in S. miltiorrihiza is well discussed. In order to investigate the stability of lithospermic acid B, the preparation samples of an injection and a granule were analyzed after being stored at 4 degrees C, 20 degrees C and 40 degrees C for up to six months.
Yuan
Dan
D
Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
Pan
Ying-ni
YN
Fu
Wen-wei
WW
Makino
Toshiaki
T
Kano
Yoshihiro
Y
D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D008517:Phytotherapy; D018517:Plant Roots; D010946:Plants, Medicinal; D027561:Salvia miltiorrhiza
D015415:Biomarkers; D004365:Drugs, Chinese Herbal