title: Structural organization and cellular localization of tuftelin-interacting protein 11 (TFIP11).
abstract: Tuftelin-interacting protein (TFIP11) was first identified in a yeast two-hybrid screening as a protein interacting with tuftelin. The ubiquitous expression of TFIP11 suggested that it might have other functions in non-dental tissues. TFIP11 contains a G-patch, a protein domain believed to be involved in RNA binding. Using a green fluorescence protein tag, TFIP11 was found to locate in a novel subnuclear structure that we refer to as the TFIP body. An in vivo splicing assay demonstrated that TFIP11 is a novel splicing factor. TFIP11 diffuses from the TFIP body following RNase A treatment, suggesting that the retention of TFIP11 is RNA dependent. RNA polymerase II inhibitor (-amanitin and actinomycin D) treatment causes enlargement in size and decrease in number of TFIP bodies, suggesting that TFIP bodies perform a storage function rather than an active splicing function. The TFIP body may therefore represent a new subnuclear storage compartment for splicing components.
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA room 103, Los Angeles, California, 90033-1004, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wen
authors.forename: Y-P
authors.initials: YP
authors.lastname: Lei
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhou
authors.forename: C T
authors.initials: CT
authors.lastname: Okamoto
authors.forename: M L
authors.initials: ML
authors.lastname: Snead
authors.forename: M L
authors.initials: ML
authors.lastname: Paine
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017398:Alternative Splicing; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042581:Intranuclear Space / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D014162:Transfection; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000546:Amanitins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins; C485860:TFIP11 protein, human; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins; D012319:RNA Polymerase II; D012259:Ribonuclease, Pancreatic