[Two-dimensional gel electrophoresis of subcellular fractions of hepatoma cells].
OBJECTIVE
To seek a better profiling of proteins of hepatoma cells.


METHODS
The homogenate of hepatoma cells QGY-7703 was fractionated into four parts by differential centrifugation: the nuclei, the pellet by 20,000 x g, the pellet by 100,000 x g and the cytosolic supernatant. The four fractions were submitted to two-dimensional gel electrophoresis and their electrophoretic patterns were analyzed.


RESULTS
In comparison with the protein pattern of hepatoma cells not fractionated, the patterns of the four fractions display many more protein spots, and a large number of proteins present in the nuclei and cytosolic supernatant were not shown in the not-fractionated samples.


CONCLUSIONS
Preparation of subcellular fractions before electrophoretic procedures proves to be very useful; not only can it improve the results of two-dimensional gel electrophoresis, but also can lead to research into the subcellular level.
Li
Xing
X
Department of Laboratory Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China.
Pan
Wei
W
Qiu
Feng
F
Tian
Kun-Lun
KL
Qiu
Zong-Yin
ZY
D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D020543:Proteome