Biological characteristics of cultured cells derived from various types of human brain tumors.
We placed in culture brain tumors from 45 cases (7 cases of astrocytoma, 2 from oligodendrogliomas, 2 glioblastomas, 2 ependymomas, 13 meningiomas, 6 pituitary adenomas, 5 neurinomas, a malignant lymphoma, a choroid plexus papilloma, and 6 metastatic tumors) and succeeded in making a primary culture from 33, and maintained 17 in vitro over a considerable period of time (greater than three months). In the early period of the primary cultures, the astrocytoma cells had cytoplasmic processes which contacted each other, the oligodendroglioma cells were small and spindle-shaped, the glioblastoma cells were neoplastic with pleopmorphic features and possessed cytoplasmic processes, the ependymoma cells formed a rosette-like cell arrangement, the meningioma cells were spindle- or round-shaped cells and characterized as forming psammoma bodies, the pituitary adenoma cells were round- or oval-shaped cells and produced growth hormone (GH), adenocorticoid tropic hormone (ACTH), prolactin, or other hypophyseal hormones, the choroid plexus papilloma cells were round-or polygonal and showed a papillary cell arrangement, the neurinoma cells were spindle- or fibrous-shaped cells, and the malignant lymphoma cells were round and formed cell aggregates floating in the culture medium.
Ishiwata
Isamu
I
Ishiwata Obstetrics & Gynecology Hospital, Mito-shi, Ibaraki-ken, Japan.
Ishiwata
Chieko
C
Iguchi
Megumi
M
Soma
Masayuki
M
Sato
Yoshiro
Y
Sonobe
Makoto
M
Kiguchi
Kazushige
K
Tachibana
Toshiaki
T
Ishikawa
Hiroshi
H
D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000473:pathology* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D003593:Cytoplasm / Q000473:pathology; D004806:Ependymoma / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D005909:Glioblastoma / Q000473:pathology / Q000648:ultrastructure; D013006:Growth Hormone / Q000096:biosynthesis; D006801:Humans; D008223:Lymphoma / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008577:Meningeal Neoplasms / Q000473:pathology; D008579:Meningioma / Q000473:pathology; D008853:Microscopy; D008875:Middle Aged; D009442:Neurilemmoma / Q000473:pathology / Q000648:ultrastructure; D009837:Oligodendroglioma / Q000473:pathology / Q000648:ultrastructure; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology; D011388:Prolactin / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013006:Growth Hormone