Precoating of alloplastic materials with living human fibroblasts--a feasibility study.
BACKGROUND
The use of alloplastic materials such as polypropylene and polyester has reduced the recurrence of abdominal wall hernias. Concomitantly, new problems have arisen such as inflammatory response against the implanted material and the development of enteric fistulas in case of direct contact of the bowel to polypropylene and polyester. A precoating of the PP with collagen and other absorbable materials seems to reduce the incidence of adhesions and fistulas. The aim of this study was to show the technical feasibility of a precoating of polypropylene with living human fibroblasts and to investigate the growth properties of the cells under these conditions in vitro.


METHODS
The textile structure of three different alloplastic materials is described (SurgiPro), TycoHealthcare; Parietene3 PP1510, Dallhausen; VIPRO II, Ethicon Endosurgery). Enhanced Green Fluorescence Protein (EGFP) transduced human foreskin fibroblasts (KiF5) were seeded onto these different alloplastic materials. Proliferation was analyzed by FACS analysis of Ki67 expression. The coating process of the whole mesh area was observed over time with UV-light microscopy, immunostaining, and scanning electron microscopy (SEM). The expression of collagen type I and III was investigated by immunostaining.


RESULTS
The three alloplastic materials used were knitted fabrics with different textile structures. KiF5 colonized the entire alloplastic material within 4-6 weeks. Cells were proliferating, as detected by Ki67 expression. SEM showed surface ruffles and long cellular extensions, indicating an active cell metabolism. Light microscopy and SEM suggested that the cells modify the apolar surface by deposition of extracellular matrix components before colonization.


CONCLUSIONS
Our study shows the feasibility of precoating of polypropylene meshes with living human fibroblasts and opens the possibility for clinical use in the future.
Kapischke
M
M
Department of Surgery, Knappschaftskrankenhaus Bochum, In der Schornau 23-25, D-44892, Bochum, Germany. mkapischke@web.de
Prinz
K
K
Tepel
J
J
Tensfeldt
J
J
Schulz
T
T
D002478:Cells, Cultured; D005240:Feasibility Studies; D005347:Fibroblasts; D046449:Hernia, Abdominal / Q000601:surgery; D006801:Humans; D011126:Polypropylenes; D013526:Surgical Mesh
D011126:Polypropylenes