Expression and regulation of CC class chemokines in the dystrophic (mdx) diaphragm.
In the murine (mdx) model of Duchenne muscular dystrophy, dystrophic changes are much more severe in the diaphragm than in limb muscles, and the diaphragm more closely resembles the human disease phenotype. Chemokines could play a central role in governing such phenotypic differences, as inflammation is an important disease modifier. Here we report that CC chemokine receptors (CCRs 1, 2, 3, 5) and ligands (macrophage inflammatory protein-1alpha, RANTES) are expressed at higher levels in dystrophic than in wild-type muscles across age groups (6, 12, and 24 wk). Moreover, chemokine ligand expression and muscle inflammation are significantly higher in dystrophic diaphragms than in limb muscles of the same animals. In vitro, CCR1 is constitutively expressed by cultured primary diaphragmatic myotubes. Stimulation of myotubes by proinflammatory cytokines (tumor necrosis factor-alpha, interleukin-1alpha, interferon-gamma) found within the in vivo dystrophic muscle environment, upregulates CCR1 in mdx and wild-type cultures, and also increases expression of its ligand RANTES to a significantly greater degree in the mdx group. Taken together, our results suggest that CC chemokines may play an important role in sustaining inflammation within the mdx diaphragm, which could help account for its more severe phenotype and also offer a target for therapeutic intervention in Duchenne patients.
Demoule
Alexandre
A
Meakins-Christie Laboratories, and Respiratory Division, McGill University Health Centre, Montreal, Quebec, Canada.
Divangahi
Maziar
M
Danialou
Gawiyou
G
Gvozdic
Dusanka
D
Larkin
Gary
G
Bao
Weisheng
W
Petrof
Basil J
BJ
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000235:genetics; D019742:Chemokines, CC / Q000235:genetics*; D016207:Cytokines / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D003964:Diaphragm / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D018836:Inflammation Mediators / Q000494:pharmacology; D008024:Ligands; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009137:Muscular Dystrophy, Animal / Q000209:etiology / Q000235:genetics* / Q000276:immunology*; D020388:Muscular Dystrophy, Duchenne / Q000209:etiology / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics
D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC; D016207:Cytokines; D018836:Inflammation Mediators; D008024:Ligands; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D004247:DNA