The Bartonella vinsonii subsp. arupensis immunodominant surface antigen BrpA gene, encoding a 382-kilodalton protein composed of repetitive sequences, is a member of a multigene family conserved among bartonella species.
Bartonella proteins that elicit an antibody response during an infection are poorly defined; therefore, to characterize antigens recognized by the host, a Bartonella genomic expression library was screened with serum from an infected mouse. This process led to the discovery of a Bartonella vinsonii subsp. arupensis gene encoding a 382-kDa protein, part of a gene family encoding large proteins, each containing multiple regions of repetitive segments. The genes were termed brpA to -C (bartonella repeat protein) and bore significant similarity to genes encoding the BadA adhesin protein and members of the variably expressed outer membrane protein family of proteins from Bartonella henselae and Bartonella quintana, respectively.
Gilmore
Robert D
RD
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Foothills Campus, Fort Collins, CO 80521, USA. rbg9@cdc.gov
Bellville
Travis M
TM
Sviat
Steven L
SL
Frace
Michael
M
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001473:Bartonella / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001474:Bartonella Infections / Q000276:immunology / Q000382:microbiology; D001483:Base Sequence; D015153:Blotting, Western; D017124:Conserved Sequence; D005455:Fluorescent Antibody Technique; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; C500905:BrpA protein, Bartonella vinsonii; D016056:Immunodominant Epitopes