Bovine glycomacropeptide is anti-inflammatory in rats with hapten-induced colitis.
Milk kappa-casein-derived glycomacropeptide has immunomodulatory and bacterial toxin binding effects. The intestinal anti-inflammatory activity of glycomacropeptide was assessed in trinitrobenzenesulfonic acid-induced colitis in rats. Rats were administered glycomacropeptide daily starting either 2 d before (pretreatment) or 3 h after (post-treatment) colitis induction. Pretreatment with glycomacropeptide had a dose-dependent anti-inflammatory effect, characterized by lower body weight loss, decreased anorexia (57%), colonic damage (65%), and weight to length ratio (32%), as well as a reduction in colonic alkaline phosphatase activity (42%) and interleukin 1, trefoil factor 3, and inducible nitric oxide synthase mRNA levels (P < 0.05). The mechanism of action of glycomacropeptide is unknown but is consistent with an inhibition of the activation of immune cells. The magnitude of the anti-inflammatory effect was generally comparable to that of sulfasalazine, an established drug used in the treatment of inflammatory bowel disease. Bovine glycomacropeptide may play a role in the management of patients with inflammatory bowel disease.
Daddaoua
Abdelali
A
Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Granada, Spain.
Puerta
Víctor
V
Zarzuelo
Antonio
A
Suárez
María D
MD
Sánchez de Medina
Fermín
F
Martínez-Augustin
Olga
O
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001483:Base Sequence; D002417:Cattle; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects; D006020:Glycopeptides / Q000627:therapeutic use*; D006241:Haptens; D007375:Interleukin-1 / Q000235:genetics; D009336:Necrosis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014302:Trinitrobenzenesulfonic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D017931:DNA Primers; D006020:Glycopeptides; D006241:Haptens; D007375:Interleukin-1; D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid