title: Methods for reducing bacterial contamination in the BacT/Alert mycobacterial culture detection system.
abstract: Two methods were compared for decreasing bacterial contamination in the BacT/Alert mycobacterial culture detection system. Two concentrations, 0.5 ml (standard amount) and 1.0 ml, of mycobacterial antibiotic supplement were evaluated. Contamination rates were 14% and 6% for the standard and the doubled concentrations, respectively. This difference was statistically significant (P < 0.005).
authors.affiliation: Division of Medical Microbiology, Johns Hopkins University, Meyer B1-193, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Kim
authors.initials: K
authors.lastname: Dionne
authors.forename: Amy
authors.initials: A
authors.lastname: Sweeney
authors.forename: Annie
authors.initials: A
authors.lastname: Hedgepeth
authors.forename: Karen
authors.initials: K
authors.lastname: Carroll
authors.forename: Nicole
authors.initials: N
authors.lastname: Parrish
mesh_terms: D004866:Equipment Contamination; D008826:Microbial Sensitivity Tests / Q000379:methods; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D014640:Vancomycin