title: Synthesis and antibody-binding studies of a series of parasite fuco-oligosaccharides.
abstract: Complex multifucosylated oligosaccharides are structural elements of glycoprotein and glycolipid subsets of larval, egg, and adult stages of Schistosoma, the parasitic worms that cause schistosomiasis, a serious disease affecting more than 200 million people in the tropics. The fucosylated structures are thought to play an important role in the immunology of schistosomiasis. Defined schistosomal oligosaccharides that enable immunological studies are difficult to obtain from natural sources. Therefore, we have chemically synthesized spacer-linked GlcNAc, Fucalpha1-3GlcNAc, Fucalpha1-2Fucalpha1-3GlcNAc, and Fucalpha1-2Fucalpha1-2Fucalpha1-3GlcNAc. This series of linear oligosaccharides was used to screen a library of anti-schistosome monoclonal antibodies by surface plasmon resonance spectroscopy. Interestingly, the reactive antibodies could be grouped according to their specificity for the different oligosaccharides tested, showing that these oligosaccharides form different immunological entities based on the number and linkage of the fucose residues. Subsequently, the thus defined monoclonal antibodies were used to visualize the expression of the corresponding oligosaccharide epitopes by adult Schistosoma mansoni worms.
authors.affiliation: Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands.
authors.forename: Anne-Marie M
authors.initials: AM
authors.lastname: van Roon
authors.forename: Begoña
authors.initials: B
authors.lastname: Aguilera
authors.forename: Francisco
authors.initials: F
authors.lastname: Cuenca
authors.forename: Alexandra
authors.initials: A
authors.lastname: van Remoortere
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Cornelis H
authors.initials: CH
authors.lastname: Hokke
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D002236:Carbohydrate Conformation; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005643:Fucose / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000254:growth & development* / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009844:Oligosaccharides; D005643:Fucose