Effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose} on human hepatocellular carcinoma cell line.
OBJECTIVE
To study the effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2).


METHODS
HepG2 cells were cultured in RPMI-1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.


RESULTS
At the concentration ranging between 1-30 micromol/L, COPADG potently inhibited the growth and induced apoptosis of HepG2 cells.


CONCLUSIONS
COPADG could effectively induce apoptosis in human hepatocellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.
Wu
Jing
J
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. wujing36@163.com
Kou
Wei
W
Gao
Ming-tai
MT
Zhou
Yong-ning
YN
Wang
Ai-qin
AQ
Xue
Qun-ji
QJ
Qiao
Liang
L
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D046529:Microscopy, Electron, Transmission
C495189:2-(3-carboxy-1-oxopropyl)amino-2-deoxy-D-glucose; D000970:Antineoplastic Agents; D003847:Deoxyglucose