Differential labeling by monoclonal antibodies Adnab-9 and anti-alpha-defensin 5 based on the distribution and adenomatous tissue content of colonic polyps.
We sought a correlation between site and morphology of colonic polyps by labeling with neoplastic and general Paneth cell markers, monoclonal antibodies Adnab-9 and anti-alpha-defensin 5, respectively. Proportions labeled by Adnab-9 and anti-alpha-defensin 5 were, respectively, 42 and 85% for adenomas, 39 and 63% for early tubular adenomas, 41 and 44% for serrated, 34 and 20% for mixed, and 11 versus 2.7% for hyperplastic polyps. Compared with hyperplastic polyps, the proportion of other polyps labeled by Adnab-9 or anti-alpha-defensin 5 was higher but this difference was more significant for distal (P = 0.008 for Adnab-9 and P = 0.0001 for anti-alpha-defensin 5) than proximal (P = 0.645 and P = 0.154, respectively) polyps. While increased labeling of all proximal polyps compared to distal ones mirrored the colonic distribution of Paneth cells, distal adenomas tended to have a higher proportion labeled by Adnab-9, suggesting that Adnab-9 labels Paneth cells associated with increased neoplastic potential.
Ullah
Nadeem
N
Departments of Internal Medicine & Pathology, John D Dingell VAMC, Detroit, Michigan 48201, USA.
Qureshi
Kashif
K
Yordanova
Violeta
V
Hatfield
James
J
Sochacki
Paula
P
Lawson
Michael
M
Tobi
Martin
M
D018256:Adenomatous Polyps / Q000473:pathology*; D000911:Antibodies, Monoclonal; D003110:Colonic Neoplasms / Q000473:pathology*; D003111:Colonic Polyps / Q000473:pathology*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019879:Paneth Cells / Q000473:pathology; D016037:Single-Blind Method; D013194:Staining and Labeling; D023084:alpha-Defensins / Q000276:immunology*
D000911:Antibodies, Monoclonal; C500225:DEFA5 protein, human; D023084:alpha-Defensins