title: Diet and biomarkers of oxidative damage in women previously treated for breast cancer.
abstract: This study sought to evaluate the relationship between dietary intake of fat, polyunsaturated fat, saturated fat, arachidonic acid, and selected dietary antioxidants and levels of oxidative damage as measured by urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-epi-prostaglandin F2alpha (8-iso-PGF2alpha) in women previously treated for breast cancer. Two hundred two study subjects participating in the Women's Healthy Eating and Living (WHEL) study were included in this ancillary study. Dietary intakes and concentrations of urinary 8-OHdG and 8-iso-PGF2alpha were measured at baseline and 12 mo in the 179 women included in the analytical cohort. Study subjects demonstrated a significant reduction in dietary total, polyunsaturated, and saturated fat intake and a significant increase in vitamins E and C and beta-carotene intake from baseline to 12 mo. Linear mixed-models analysis using baseline and Year 1 data indicated that vitamin E intake was inversely associated with both 8-OHdG and 8-iso-PGF2alpha. 8-Iso-PGF2alpha is increased with increased body mass index (BMI) and polyunsaturated fatty acid (PUFA) intake, indicating an increase in lipid peroxidation with greater BMI and higher PUFA intake. 8-OHdG was inversely related to age but positively related to arachidonic acid, indicating an increase in DNA damage with higher intake of arachidonic acid (meat). The results of this nested case-controlled study provide potential mechanisms by which a high fruit and vegetable, low-fat diet might reduce the recurrence rate of or early-stage breast cancer.
authors.affiliation: Department of Nutritional Sciences, The University of Arizona, Tucson 85721-0038, USA. cthomson@email.arizona.edu
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Thomson
authors.forename: Anna R
authors.initials: AR
authors.lastname: Giuliano
authors.forename: James W
authors.initials: JW
authors.lastname: Shaw
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Rock
authors.forename: Cheryl K
authors.initials: CK
authors.lastname: Ritenbaugh
authors.forename: Iman A
authors.initials: IA
authors.lastname: Hakim
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Hollenbach
authors.forename: David S
authors.initials: DS
authors.lastname: Alberts
authors.forename: John P
authors.initials: JP
authors.lastname: Pierce
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants / Q000008:administration & dosage; D015415:Biomarkers / Q000652:urine; D015992:Body Mass Index; D001943:Breast Neoplasms / Q000517:prevention & control* / Q000652:urine; D016022:Case-Control Studies; D015331:Cohort Studies; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000652:urine; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D055502:Secondary Prevention; D013997:Time Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine