Intein-mediated protein trans-splicing and transgene containment in plastids.
Transgenes in plastids are contained by stringent maternal inheritance in most cultivated plant species and their expression yields high levels of protein with bona fide structure. Nevertheless, transfer of plastid genes to the nucleus has been reported, with implications for transgene containment. The significance of these transfers will depend on the likelihood that they will become functional nuclear genes. Recently a novel approach, intein-mediated protein trans-splicing, has been demonstrated promising to yield transgenic plants with greatly reduced risk of genetic outcrossing.
Khan
Muhammad Sarwar
MS
National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad 38000, Pakistan. sarwarkhan_40@hotmail.com
Khalid
Ahmad M
AM
Malik
Kauser A
KA
D047668:Inteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D018087:Plastids / Q000235:genetics*; D019154:Protein Splicing / Q000235:genetics*; D019076:Transgenes / Q000235:genetics