title: Role of HdeA in acid resistance and virulence in Brucella abortus 2308.
abstract: Two-dimensional gel electrophoretic analysis of cell lysates suggests that stationary phase production of wild-type levels of an ortholog of the low pH dependent chaperone HdeA in Brucella abortus 2308 during growth in a minimal medium requires the presence of the RNA binding protein Hfq. Although mutational analysis demonstrated that HdeA contributes to acid resistance in this bacterium, this protein is not required for wild-type virulence in the BALB/c mouse model. These experimental findings indicate that the brucellae rely upon additional gene products to resist the acidic conditions they encounter in the phagosomal compartment of host macrophages.
authors.affiliation: Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27834, USA.
authors.forename: Michelle Wright
authors.initials: MW
authors.lastname: Valderas
authors.forename: Rosemarie B
authors.initials: RB
authors.lastname: Alcantara
authors.forename: John E
authors.initials: JE
authors.lastname: Baumgartner
authors.forename: Bryan H
authors.initials: BH
authors.lastname: Bellaire
authors.forename: Gregory T
authors.initials: GT
authors.lastname: Robertson
authors.forename: Wai-Leung
authors.initials: WL
authors.lastname: Ng
authors.forename: John M
authors.initials: JM
authors.lastname: Richardson
authors.forename: Malcolm E
authors.initials: ME
authors.lastname: Winkler
authors.forename: R Martin
authors.initials: RM
authors.lastname: Roop
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002003:Brucella abortus / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D002006:Brucellosis / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D035001:Host Factor 1 Protein / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014774:Virulence
chemical_list: D035001:Host Factor 1 Protein