title: [Construction of flocculation selective vector and expression of beta-glucosidase gene in Saccharomyces cerevisiae].
abstract: Selective markers used in yeast vector for gene manipulation were usually drug resistance or autotrophy. Unfortunately, drug resistance selective marker requires drug sensitive host and most industrial strains were not autotrophy. In this paper, flocculation gene (FLO1) from Saccharomyces cerevisiae ABXL-1D was amplified by PCR, sequenced and cloned to construct an expression vector. The new vector was easy to manipulate and suitable for broad host of yeasts without either autotrophy or drugs. beta-glucosidase gene from Bacillus polymyxa was cloned with the vector and expressed in Saccharomyces cerevisiae. The specific activity of beta-glucosidase of the recombinant yeast cell-free extract was 3.91 u/mg protein. The residue glucose of the recombinant yeast was considerably reduced in mixed fermentation of glucose and cellobiose. It should be favorable for ethanol fermentation when utilize lignocellulosic biomass as raw material.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: Da-Jun
authors.initials: DJ
authors.lastname: Lu
authors.forename: An
authors.initials: A
authors.lastname: Shen
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D005427:Flocculation; D005822:Genetic Vectors / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D001617:beta-Glucosidase