Cardiac neural crest is necessary for normal addition of the myocardium to the arterial pole from the secondary heart field.
In cardiac neural-crest-ablated embryos, the secondary heart field fails to add myocardial cells to the outflow tract and elongation of the tube is deficient. Since that study, we have shown that the secondary heart field provides both myocardium and smooth muscle to the arterial pole. The present study was undertaken to determine whether addition of both cell types is disrupted after neural crest ablation. Marking experiments confirm that the myocardial component fails to be added to the outflow tract after neural crest ablation. The cells destined to go into the outflow myocardium fail to migrate and are left at the junction of the outflow myocardium with the nascent smooth muscle at the base of the arterial pole. In contrast, the vascular smooth muscle component is added to the arterial pole normally after neural crest ablation. When the myocardium is not added to the outflow tract, the point where the outflow joins the pharynx does not move caudally as it normally should, the aortic sac is smaller and fails to elongate resulting in abnormal connections of the outflow tract with the caudal aortic arch arteries.
Waldo
Karen L
KL
Department of Pediatrics (Neonatology), Neonatal-Perinatal Research Institute, Duke University Medical Center, Bell Building, Room 154, Box 3179, Durham, NC 27710, USA.
Hutson
Mary R
MR
Stadt
Harriett A
HA
Zdanowicz
Marzena
M
Zdanowicz
Jaroslaw
J
Kirby
Margaret L
ML
D000818:Animals; D001011:Aorta / Q000033:anatomy & histology / Q000196:embryology; D015415:Biomarkers; D018959:CD57 Antigens / Q000378:metabolism; D049109:Cell Proliferation; D002642:Chick Embryo; D006321:Heart / Q000033:anatomy & histology* / Q000196:embryology*; D017403:In Situ Hybridization; D008840:Microfilament Proteins / Q000378:metabolism; D009024:Morphogenesis; D009124:Muscle Proteins / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism*; D010614:Pharynx / Q000033:anatomy & histology / Q000196:embryology
D015415:Biomarkers; D018959:CD57 Antigens; D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin