Determination of bovine rotavirus G genotypes in Kashmir, India.
Rotavirus ribonucleic acid (RNA) was extracted from ten faecal samples of diarrhoeic calves positive for group A rotavirus by enzyme-linked immunosorbent assay (ELISA). A portion of the extracted RNA was run in polyacrylamide gel to determine the presence of rotaviral RNA and the rest subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) to generate the near full length VP7 gene. Only six samples yielded the desired product. The amplified products were subjected to G-typing by PCR using a cocktail of G6, G8 and G10 typing primers. All of the six samples were characterised as G10 and none of the samples revealed mixed infection by twin G types. Four samples, despite possessing sufficient rotavirus particles as revealed by ELISA and polyacrylamide gel electrophoresis, did not yield any amplified product on RT-PCR. This could be due to non-specific inhibitors of the PCR reaction, present in the faecal samples, being carried through the extraction procedures.
Wani
S A
SA
Rotavirus Laboratory, SK University of Agricultural Sciences and Technology of Kashmir, Shuhama, (Alusteng) Srinagar-190006, India.
Bhat
M A
MA
Ishaq
S M
SM
Ashrafi
M A
MA
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005243:Feces / Q000821:virology; D005838:Genotype; D007194:India; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
D012367:RNA, Viral