title: The missense mutations in the candidate prostate cancer gene ELAC2 do not alter enzymatic properties of its product.
abstract: The candidate prostate cancer gene ELAC2 encodes tRNA 3' processing endoribonuclease (tRNase ZL). We produced recombinant human tRNase ZL's, which contain one to three amino-acid substitutions from three missense mutations (Ser217Leu, Ala541Thr, and Arg781His) that are associated with the occurrence of prostate cancer. These enzymes were examined for the pre-tRNA cleavage and the RNase 65 activity. We did not observe any differences in enzymatic properties such as Km and k(cat) values between the wild-type tRNase ZL and its variants. We conclude that there is no causality between the enzymatic properties of tRNase ZL and the prostate cancer.
authors.affiliation: Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niitsu, Niigata 956-8603, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Minagawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takaku
authors.forename: Masamichi
authors.initials: M
authors.lastname: Takagi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nashimoto
mesh_terms: D004722:Endoribonucleases / Q000235:genetics* / Q000494:pharmacology*; D006801:Humans; D006867:Hydrolases; D007700:Kinetics; D008297:Male; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000235:genetics* / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000235:genetics*
chemical_list: C421605:ELAC2 protein, human; D009363:Neoplasm Proteins; D006867:Hydrolases; D004722:Endoribonucleases; C045782:3'-pre-tRNase