AIP1, a water-soluble fraction from Artemisia iwayomogi, suppresses thymocyte apoptosis in vitro and down-regulates the expression of Fas gene.
The AIP1 fraction, a small water-soluble fraction purified from Artemisia iwayomogi, was shown to increase antibody production and suppress transplanted tumor cell growth in mice. In order to understand its immuno-modulating activity, we have examined the effect of the AIP1 on mouse thymocytes in vitro. Treatment of mouse thymocytes in culture with the fraction resulted in the suppression of the cell death and the extension of the cell survival. A mouse gene array provided a profile of gene expression change showing the pattern of up- and down-regulated genes by the AIP1 treatment, suggesting that the Fas/FasL-dependent apoptosis pathway might be modulated by the fraction.
Hwang
Jung Suk
JS
School of Biotechnology & Biomedical Science and Bio-Health Product Research Center, Inje University, Gimhae 621-749, Korea.
Ji
Hee Jung
HJ
Koo
Kyung Ah
KA
Lee
Nam Hee
NH
Yeo
Hee Kyoung
HK
Cheong
Seon Woo
SW
Park
Joo Hung
JH
Oh
Gap Soo
GS
Yoon
Chun Sik
CS
Youn
Hyun Joo
HJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D019306:Artemisia; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005591:Chemical Fractionation; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007155:Immunologic Factors / Q000302:isolation & purification / Q000502:physiology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012995:Solubility; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors / Q000096:biosynthesis*; D014867:Water
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007155:Immunologic Factors; D008562:Membrane Glycoproteins; D010936:Plant Extracts; D000079424:Tumor Necrosis Factor Inhibitors; D048069:Tumor Necrosis Factors; D014867:Water