title: Inhibition of angiogenic activity of renal carcinoma by an antisense oligonucleotide targeting fibroblast growth factor-2.
abstract: BACKGROUND
  Fibroblast growth factor-2 (FGF-2) induces angiogenesis, critical for the growth and metastatic spread of tumors.
  
  
  METHODS
  The effect of blocking FGF-2 synthesis by an antisense phosphorothioate oligodeoxynucleotide (PS-ODN2) was evaluated on the angiogenic activity of Caki-1 and of a cell line isolated from a renal carcinoma bone metastasis (CRBM-1990). After the transfection with PS-ODN2, FGF-2 mRNA, protein expression and angiogenic activity were evaluated.
  
  
  RESULTS
  In Caki-1, a not significant decrease in the released FGF-2 was observed after 72 hours. In CRBM-1990, a not significant decrease in intracellular FGF-2 protein was observed after 72 hours. Endothelial cell migration induced by the conditioned media from Caki-1 treated with PS-ODN2 for 72 hours was significantly reduced.
  
  
  CONCLUSIONS
  PS-ODN2 treatment of the established line Caki-1 induced minimal variations in FGF-2 expression, but inhibited endothelial cell migration. In CRBM-1990 cells, PS-ODN2 determined a decrease in intracellular protein without reducing the ability to induce endothelial cell migration and proliferation.
authors.affiliation: Laboratory for Pathophysiology of Orthopedic Implants, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cenni
authors.forename: Francesca
authors.initials: F
authors.lastname: Perut
authors.forename: Monia
authors.initials: M
authors.lastname: Zuntini
authors.forename: Donatella
authors.initials: D
authors.lastname: Granchi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Amato
authors.forename: Sofia
authors.initials: S
authors.lastname: Avnet
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Brandi
authors.forename: Armando
authors.initials: A
authors.lastname: Giunti
authors.forename: Nicola
authors.initials: N
authors.lastname: Baldini
mesh_terms: D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000098:blood supply* / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002633:Chemotaxis / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000098:blood supply* / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000517:prevention & control*; D016376:Oligonucleotides, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2