Generation and characterization of Rgs4 mutant mice.
RGS proteins are negative regulators of signaling through heterotrimeric G protein-coupled receptors and, as such, are in a position to regulate a plethora of biological phenomena. However, those have just begun to be explored in vivo. Here, we describe a mouse line deficient for Rgs4, a gene normally expressed early on in discrete populations of differentiating neurons and later on at multiple sites of the central nervous system, the cortex in particular, where it is one of the most highly transcribed Rgs genes. Rgs4(lacZ/lacZ) mice had normal neural development and were viable and fertile. Behavioral testing on mutant adults revealed subtle sensorimotor deficits but, so far, supported neither the proposed status of Rgs4 as a schizophrenia susceptibility gene (by showing intact prepulse inhibition in the mutants) nor (unlike another member of the Rgs family, Rgs9) a role of Rgs4 in the acute or chronic response to opioids.
Grillet
Nicolas
N
CNRS UMR 8542, Department of Biology, Ecole Normale Supérieure, 46 rue d'Ulm, 75005, Paris, France.
Pattyn
Alexandre
A
Contet
Candice
C
Kieffer
Brigitte L
BL
Goridis
Christo
C
Brunet
Jean-François
JF
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001831:Body Temperature; D001835:Body Weight; D002454:Cell Differentiation; D003214:Conditioning, Classical / Q000502:physiology; D005239:Fear / Q000502:physiology; D005260:Female; D017353:Gene Deletion; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009020:Morphine / Q000009:adverse effects; D009270:Naloxone / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology; D009460:Neurologic Examination; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D017288:Pain Threshold; D010641:Phenotype; D020710:RGS Proteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D013216:Reflex, Startle; D012559:Schizophrenia / Q000209:etiology / Q000235:genetics; D013375:Substance Withdrawal Syndrome / Q000209:etiology / Q000503:physiopathology
D009294:Narcotics; D020710:RGS Proteins; C098799:RGS4 protein; D009270:Naloxone; D009020:Morphine