[The study on platelet-derived growth factor and proliferating cell nuclear antigen antisense oligodeoxynucleotides together inhibiting the stenosis of transplanted vascular].
OBJECTIVE
To study the effect of platelet-derived growth factor (PDGF) and proliferating cell nuclear antigen (PCNA) antisense oligodeoxynucleotides (AODN) together on inhibiting the proliferation of the stenosis of transplanted vascular.


METHODS
The left and right external iliac arteries (length 1.0 cm) of rabbits were transplanted reciprocally. The transplanted vascular were respectively soaked in liposomes, PDGF-AODN, PCNA-AODN and PDGF-AODN adding PCNA-AODN solution about 20 minute, the vascular anastomotic were sutured by 8/0 suture of soaked in AODN solution. Four weeks later, the specimens were harvested for microscopy. The pathological morphology of transplanted vascular were observed under microscope (HE). The intimal thickness and area, stenosis ratio(%) of transplanted vascular were calculate and analysed statistically among group by computer system. The number of positive cells of PDGF's mRNA in transplanted vascular wall were counted with in situ hybridization histo-cytochemistry and the number of positive cells of PCNA's protein in transplanted vascular wall were counted by S-P immunochemistry.


RESULTS
The intimal thickness and area, stenosis ratio of transplanted vascular, the number of PDGF and PCNA positive cell in PDGF-AODN adding PCNA-AODN group were significantly lower than those in other group (P < 0.01), and that were lower evidently than PDGF-AODN group and PCNA-AODN group.


CONCLUSIONS
PDGF and PCNA antisense oligodeoxynucleotides together could significantly inhibit the proliferation of vascular smooth muscle cell and stenosis of transplanted vascular.
Wu
Zhong-Jun
ZJ
The Organ Grafting Center, First Affiliated Hospital, Zhejiang University, Hangzhou 310012, China. wzjxdfpxy@zju.edu.cn
Zhu
Yi
Y
Shi
De
D
Zheng
Shu-sen
SS
Li
De-wei
DW
D000818:Animals; D006083:Graft Occlusion, Vascular / Q000473:pathology / Q000517:prevention & control*; D007083:Iliac Artery / Q000473:pathology / Q000637:transplantation*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics*; D011817:Rabbits; D014162:Transfection; D014184:Transplantation, Homologous
D016376:Oligonucleotides, Antisense; D010982:Platelet-Derived Growth Factor; D018809:Proliferating Cell Nuclear Antigen