title: A high-fat diet generates alterations in nuclear receptor expression: prevention by vitamin A and links with cyclooxygenase-2 and beta-catenin.
abstract: Epidemiologic studies suggest that intake of high energy from fat, inducing overweight, increases the risk of cancer development and promotes colon carcinogenesis. It is therefore important to understand which parameters are affected early on by a high-fat diet in order to devise and improve protective nutritional strategies. We investigated the effect of high energy/fat intake on colon mucosa of male Wistar rats induced by a single 1,2-dimethylhydrazine (DMH) injection. Aberrant crypt foci (ACF) were numbered and modifications in cyclooxygenase-2 (COX-2) and beta-catenin levels assessed. Peroxisome proliferator- and retinoic acid-activated receptors (PPAR and RAR, RXR) are key transcription factors regulating gene expression in response to nutrient-activated signals. A short-term study was designed to evaluate whether alterations in mRNA expression of nuclear receptors can be detected at the beginning of the weight gain phase induced by an appetizing hyperlipidic diet (HLD). HLD consumption induced early downregulation of PPARgamma (-33.1%) and RARbeta (-53.1%) mRNA expression concomitant with an increase in levels of COX-2 (+45.5%) and beta-catenin (+84.56%) and in the number of ACF (191.56 +/- 88.60 vs. 21.14 +/- 11.64, p < 0.05). These findings suggest that HLD increases ACF occurrence, possibly through alterations in the mRNA expression profile of nuclear receptors. Moreover, the use HLD rich in retinyl esters or supplemented with all-trans retinoic acid led to a reduction in the number of ACF. Vitamin A also prevented HLD-induced alterations and the increase in levels of COX-2 and beta-catenin. The present observations show a protective role for vitamin A against disturbances associated with HLD exposure in induced colon carcinogenesis.
authors.affiliation: Laboratory of Food and Colon Carcinogenesis, Unit of Nutrition and Cellular Signalling, University Bordeaux 1, Talence, France. delage.barbara@laposte.net
authors.forename: Barbara
authors.initials: B
authors.lastname: Delage
authors.forename: Céline
authors.initials: C
authors.lastname: Bairras
authors.forename: Benjamin
authors.initials: B
authors.lastname: Buaud
authors.forename: Véronique
authors.initials: V
authors.lastname: Pallet
authors.forename: Pierrette
authors.initials: P
authors.lastname: Cassand
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D051546:Cyclooxygenase 2; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000235:genetics*; D018168:Receptors, Retinoic Acid / Q000187:drug effects / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology; D014801:Vitamin A / Q000494:pharmacology*; D051176:beta Catenin
chemical_list: D002273:Carcinogens; C495268:Ctnnb1 protein, rat; D003598:Cytoskeletal Proteins; D004041:Dietary Fats; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D015534:Trans-Activators; D051176:beta Catenin; D014801:Vitamin A; D014212:Tretinoin; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D019813:1,2-Dimethylhydrazine