title: Simultaneous analysis of multiple classes of antibiotics by ion trap LC/MS/MS for assessing surface water and groundwater contamination.
abstract: Solid-phase extraction (SPE) and liquid chromatography in combination with ion trap mass spectrometry (LC/MS/MS) conditions were optimized for the simultaneous analysis of 13 antibiotics belonging to multiple classes and caffeine in 3 different water matrixes. The single-cartridge extraction step was developed using a reversed-phase cartridge, resulting in recoveries for the 14 compounds ranging from 71 to 119% with relative standard deviations of 16% or lower. The analytes were separated in one chromatographic run, and the SPE-LC/MS/MS detection limits ranged from 0.03 to 0.19 microg/L. The SPE procedure was validated in groundwater, surface water, and wastewater. The analysis of samples from each of the three water matrixes revealed clindamycin (1.1 microg/L) in surface water and multiple antibiotics in wastewater (0.10-1.3 microg/L). The use of identification points to unambiguously assign the identity of antibiotics in various water matrixes was applied to an ion trap data-dependent scanning method, which simultaneously collects full scan and full scan MS/MS data for the unequivocal identification of target analytes.
authors.affiliation: Department of Chemistry, State University of New York at Buffalo, 608 Natural Sciences Complex, Buffalo, New York 14260-3000, USA.
authors.forename: Angela L
authors.initials: AL
authors.lastname: Batt
authors.forename: Diana S
authors.initials: DS
authors.lastname: Aga
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002110:Caffeine / Q000032:analysis; D002853:Chromatography, Liquid; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000032:analysis; D045483:Rivers / Q000737:chemistry; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D014874:Water Pollutants, Chemical; D002110:Caffeine