[Effect of fluoride on activities of enzyme and ultrastructure in primary cultured rat hepatocytes].
OBJECTIVE
To study the cell viability, activities of enzyme and ultrastructure changes induced by sodium fluoride in primary cultured rat hepatocytes.


METHODS
Hepatocytes were isolated using half-in situ collagenase digestion method. Cellular viability was determined by MTT method. The activities of ALT and AST were determined by spectrophotography method. The ultrastructure changes of hepatocyte were observed under transmission electron microscope.


RESULTS
After cultured with various concentrations of fluoride for 24 hours, a dose-dependent decrease of cell viability was detected in the hepatocytes. The activities of AST and ALT in the 2 mmol/L and 4 mmol/L groups were significantly higher than the control group (P < 0.05). Transmission electron microscope study showed that in fluoride treated hepatocytes the changes included swollen mitochondria and disordered, disrupted endoplasm reticulum.


CONCLUSIONS
Excessive fluoride induced significant toxicity in primary cultured hepatocytes which manifested the injuries of membrane and organell plasma membrane.
Guo
Xiao-Ying
XY
School of Public Health, China Medical University, Shenyang 110001, China.
Sun
Ying-Chun
YC
Sun
Gui-Fan
GF
D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*
D012969:Sodium Fluoride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase