A genetic locus of Helicobacter pylori inversely associated with gastric intestinal metaplasia.
The genomic contents of Helicobacter pylori strain C1 from a patient with gastric cancer and strain 98587 from a patient with duodenal ulcer disease were compared using a rapid subtractive hybridisation approach. A total of 11 tester-specific sequences representing gene specificity, DNA rearrangement and sequence variation were identified. This included two novel sequences, clone P32 and clone F5, which have no significant homologue in the database. H. pylori strains positive for clone P32 were less prevalent in patients with gastric intestinal metaplasia (12.5%) than in duodenal ulcer (39.1%) (p=0.036), or chronic gastritis (38.1%) (p=0.036). The results suggest that H. pylori clone P32 is potentially a useful marker for distinguishing intestinal metaplasia associated strains from others.
Dong
Quanjiang
Q
Research Laboratories, Trinity Health Centre at Adelaide and Meath Hospital, Trinity College Dublin, Dublin, Ireland.
O'Sullivan
Maria
M
Nami
Abdurrazag
A
Dowling
Paul
P
Murphy
Gwen
G
Buckley
Martin
M
O'Morain
Colm
C
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002908:Chronic Disease; D003001:Cloning, Molecular; D004381:Duodenal Ulcer / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000235:genetics / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D007422:Intestines / Q000473:pathology*; D008679:Metaplasia / Q000235:genetics* / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori; C503476:clone p32 protein, Helicobacter pylori