title: [Two-dimensional gel electrophoresis of subcellular fractions of hepatoma cells].
abstract: OBJECTIVE
  To seek a better profiling of proteins of hepatoma cells.
  
  
  METHODS
  The homogenate of hepatoma cells QGY-7703 was fractionated into four parts by differential centrifugation: the nuclei, the pellet by 20,000 x g, the pellet by 100,000 x g and the cytosolic supernatant. The four fractions were submitted to two-dimensional gel electrophoresis and their electrophoretic patterns were analyzed.
  
  
  RESULTS
  In comparison with the protein pattern of hepatoma cells not fractionated, the patterns of the four fractions display many more protein spots, and a large number of proteins present in the nuclei and cytosolic supernatant were not shown in the not-fractionated samples.
  
  
  CONCLUSIONS
  Preparation of subcellular fractions before electrophoretic procedures proves to be very useful; not only can it improve the results of two-dimensional gel electrophoresis, but also can lead to research into the subcellular level.
authors.affiliation: Department of Laboratory Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Kun-Lun
authors.initials: KL
authors.lastname: Tian
authors.forename: Zong-Yin
authors.initials: ZY
authors.lastname: Qiu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome