Chiral liquid chromatography-tandem mass spectrometric methods for stereoisomeric pharmaceutical determinations.
The characterization of the drug metabolism and pharmacokinetic (DMPK) profiles of stereoisomers is a fundamental aspect of the drug discovery and development processes. Therefore, chiral drug bioassays are very important to pharmaceutical and biomedical researchers. The recent developments in chiral liquid chromatography coupled to atmospheric pressure ionization tandem mass spectrometry (LC-API-MS/MS) for the analysis of pharmaceuticals are reviewed. Various ionization techniques including electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric photoionization (APPI) interfaced with chiral liquid chromatographic methods are described in terms of their ionization efficiencies, matrix effects and limitations. Examples were selected to demonstrate the applicability of these methods for enantioselective bioanalysis.
Chen
Jiwen
J
Drug Metabolism and Pharmacokinetics Department, 2015 Galloping Hill Road, K-15-3700, Kenilworth, NJ 07033, USA.
Korfmacher
Walter A
WA
Hsieh
Yunsheng
Y
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods / Q000639:trends; D013058:Mass Spectrometry / Q000379:methods / Q000639:trends; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations