Identification of a peach latent mosaic viroid hairpin able to act as a Dicer-like substrate.
The ability of several viroids to induce posttranscriptional gene silencing has been demonstrated; however, the structure recognized by the Dicer enzyme(s) responsible for the initiation of this mechanism remains a mystery. Here, we show that the hairpin known to be implicated in the replication of peach latent mosaic viroid has the ability to trigger the Dicer enzyme(s). This domain, which is composed of a succession of several small stems separated by symmetrical bulges, is reminiscent of the precursor micro-RNAs.
Landry
Patricia
P
RNA Group/Groupe ARN, Département de Biochimie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada.
Perreault
Jean-Pierre
JP
D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010936:Plant Extracts; D027861:Prunus / Q000821:virology*; D034622:RNA Interference; D014908:Triticum / Q000201:enzymology; D014772:Viroids / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D010936:Plant Extracts