title: Expression of Xenopus XlSALL4 during limb development and regeneration.
abstract: The multi-C2H2 zinc-finger domain containing transcriptional regulators of the spalt (SAL) family plays important developmental regulatory roles. In a competitive subtractive hybridization screen of genes expressed in Xenopus laevis hindlimb regeneration blastemas, we identified a SAL family member that, by phylogenetic analysis, falls in the same clade as human SALL4 and have designated it as XlSALL4. Mutations of human SALL4 have been linked to Okihiro syndrome, which includes preaxial (anterior) limb defects. The expression pattern of XlSALL4 transcripts during normal forelimb and hindlimb development and during hindlimb regeneration at the regeneration-competent and regeneration-incompetent stages is temporally and regionally dynamic. We show for the first time that a SAL family member (XlSALL4) is expressed at the right place and time to play a role regulating both digit identity along the anterior/posterior axis and epimorphic limb regeneration.
authors.affiliation: Medical Sciences Program, Indiana University School of Medicine, Bloomington, Indiana 47405, USA. neff@indiana.edu
authors.forename: Anton W
authors.initials: AW
authors.lastname: Neff
authors.forename: Michael W
authors.initials: MW
authors.lastname: King
authors.forename: Mark W
authors.initials: MW
authors.lastname: Harty
authors.forename: Trent
authors.initials: T
authors.lastname: Nguyen
authors.forename: John
authors.initials: J
authors.lastname: Calley
authors.forename: Rosamund C
authors.initials: RC
authors.lastname: Smith
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Mescher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006614:Hindlimb / Q000737:chemistry / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012038:Regeneration / Q000502:physiology*; D016415:Sequence Alignment; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*
chemical_list: C503712:SALL4 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins