The interleukin-6--174 single nucleotide polymorphism: cervical protein production and the risk of preterm delivery.
OBJECTIVE
The purpose of this study was to determine the associations between preterm delivery (PTD), cervical fluid interleukin-6 (IL-6) concentration, and the single nucleotide polymorphism at position -174 in the IL-6 gene.


METHODS
Cervical fluid samples were obtained from women 23 to 32 weeks' gestation with symptoms of preterm labor. Concentrations of IL-6 were determined by enzyme-linked immunosorbent assay (ELISA). IL-6 genotyping was performed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.


RESULTS
One hundred thirty-seven women were enrolled, and complete information was available for 126. Cervical fluid IL-6 concentrations were not elevated in women destined to have a spontaneous PTD ( P = .86). IL-6 -174 genotype was not associated with PTD ( P = .62) or cervical fluid IL-6 concentration ( P = .36). Neonatal IL-6-174 genotype was not associated with PTD or IL-6 concentration.


CONCLUSIONS
Cervical fluid concentrations of IL-6 were not elevated in symptomatic women destined to have a spontaneous PTD. The presence of maternal IL-6 -174C was not associated with cervical fluid concentration of IL-6 or risk of PTD.
Jamie
Whitney E
WE
Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, FL, USA.
Edwards
Rodney K
RK
Ferguson
Ronald J
RJ
Duff
Patrick
P
D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D002584:Cervix Uteri / Q000378:metabolism*; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics; D008969:Molecular Sequence Data; D007752:Obstetric Labor, Premature / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011256:Pregnancy Outcome; D011336:Probability; D011446:Prospective Studies; D018570:Risk Assessment; D012680:Sensitivity and Specificity
D015415:Biomarkers; D015850:Interleukin-6