[Development of DNA array filter useful for the analysis of Th1/Th2 balance].
DNA arrays are useful for determining the expression levels of a number of genes at once. We utilized this technique to evaluate the Th1/Th2 balance in vivo. Immune responses are controlled by two types of helper T cells, Th1 and Th2. Once the balance of Th1/Th2 immunity is disrupted, various immune diseases can develop. Thus, it is important to evaluate the Th1/Th2 balance in each patient for diagnosis, treatment and/or prophylaxis of immune diseases. We have identified a number of genes specifically expressed in Th1 or Th2 cells, and developed a DNA array filter spotted with these genes. We confirmed that this filter is useful for the evaluation of changes in the immune balance in vivo. Clinical application of this technology may lead to the tailor-made therapy of immune diseases through the evaluation of the immune balance in each patient.
Yamaguchi
Aki
A
Division of immunoregulation, Institute for Genetic Medicine, Hokkaido University.
Togashi
Yuji
Y
Koda
Toshiaki
T
Nishimura
Takashi
T
D000199:Actins / Q000235:genetics; D000465:Algorithms; D000818:Animals; D000917:Antibody Formation; D020869:Gene Expression Profiling; D006086:Graft vs Host Disease / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D000199:Actins