Delayed repair of corticospinal tract lesions as an assay for the effectiveness of transplantation of Schwann cells.
We have previously shown that cultured adult olfactory ensheathing cells injected after 8 weeks into functionally complete unilateral lesions of the rat corticospinal tract induce restoration of paw reaching function to about 50% of normal, starting at around 10 days after transplantation. This provides an assay for determining the effectiveness of different methods of cell preparation or different cell types. We report that transplantation of cultured adult peripheral nerve Schwann cells also restores function, but the effect is delayed until around 30 days after transplantation and reaches only around 5-10% of normal. The presence of fibroblasts in the Schwann cell cultures neither improves, nor impairs the reparative effect, but fibroblasts alone (without Schwann cells) have no reparative effect. Without transplantation of exogenous Schwann cells, the ingrowth of endogenous Schwann cells which occurs spontaneously into these lesions does not restore function.
Keyvan-Fouladi
Naghmeh
N
Spinal Repair Unit, Institute of Neurology, University College London, London, United Kingdom.
Raisman
Geoffrey
G
Li
Ying
Y
D000818:Animals; D001681:Biological Assay; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000502:physiology / Q000637:transplantation / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009069:Movement Disorders / Q000209:etiology / Q000503:physiopathology / Q000628:therapy; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D011712:Pyramidal Tracts / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D020127:Recovery of Function / Q000502:physiology; D012583:Schwann Cells / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013997:Time Factors; D016378:Tissue Transplantation / Q000379:methods* / Q000639:trends; D016896:Treatment Outcome