Drifter, a novel, low copy hAT-like transposon in Fusarium oxysporum is activated during starvation.
The facultative pathogenic fungus Fusarium oxysporum is known to harbour many different transposable and/or repetitive elements. We have identified Drifter, a novel DNA transposon of the hAT family in F. oxysporum. It was found adjoining SIX1-H, a truncated homolog of the SIX1 avirulence gene in F. oxysporum f. sp. lycopersici. Absence of a target site duplication as well as the 5' part of SIX1-H suggests that transposition of Drifter into the ancestor of SIX1-H was followed by loss of a chromosomal segment through recombination between Drifters. F. oxysporum isolates belonging to various formae speciales harbour between 0 and 5 full-length copies of Drifter and/or one or more copies with an internal deletion. Transcription of Drifter is activated during starvation for carbon or nitrogen.
Rep
Martijn
M
Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, P.O. Box 94062, 1090 GB Amsterdam, The Netherlands. m.rep@uva.nl
van der Does
H Charlotte
HC
Cornelissen
Ben J C
BJ
D004251:DNA Transposable Elements / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000032:analysis; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D014158:Transcription, Genetic; D014774:Virulence
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D012331:RNA, Fungal