title: Generation of dendritic cells from human peripheral blood monocytes--comparison of different culture media.
abstract: Culture medium or medium supplement is one of the factors responsible for dendritic cell (DC) generation, but little is known about the influence of various media on DC culture. In our study we generated DC from adherent monocytes of human peripheral blood in the presence of GM-CSF, IL-4 and TNF-alpha. The following culture media were used: RPMI 1640 supplemented with 2% human serum albumin; RPMI 1640 supplemented with 2% TCH serum replacement; X-VIVO 15 and Panserin 501. Flow cytometry analysis revealed that in all media cells were CD83+ and lost CD14. Interestingly, the use of Panserin and RPMI with albumin preferentially gave rise to CD1a+ DC, whereas in X-VIVO and RPMI with TCH we observed both CD1a+ and CD1a-. Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both CD80 and CD86 molecules and, in contrast to other media, the higher percentage of CD86+ cells in comparison to CD80+ cells.
authors.affiliation: Department of Clinical Immunology, Medical University, Lublin, Poland. natkaukr@yahoo.com
authors.forename: Nataliya
authors.initials: N
authors.lastname: Tkachenko
authors.forename: Kamila
authors.initials: K
authors.lastname: Wojas
authors.forename: Jacek
authors.initials: J
authors.lastname: Tabarkiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Rolinski
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D012016:Reference Values
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D003470:Culture Media; D008562:Membrane Glycoproteins