Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies virus proteins produced in Escherichia coli.
We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection.
Motoi
Yurie
Y
The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.
Inoue
Satoshi
S
Hatta
Hajime
H
Sato
Kozue
K
Morimoto
Kinjiro
K
Yamada
Akio
A
D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens; D004926:Escherichia coli; D005260:Female; D007136:Immunoglobulins / Q000276:immunology*; D030781:Organisms, Genetically Modified; D011818:Rabies / Q000175:diagnosis; D011820:Rabies virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000956:Antigens, Viral; C016512:IgY; D007136:Immunoglobulins; D011994:Recombinant Proteins