Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods.
Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results in 60 of the 61 isolates.
Tsang
Raymond S W
RS
Vaccine Preventable Bacterial Diseases Division, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada. rtsang@hc-sc.gc.ca
Sill
Michelle L
ML
Advani
Abdolreza
A
Xing
Dorothy
D
Newland
Penny
P
Hallander
Hans
H
D000372:Agglutination Tests / Q000379:methods; D000911:Antibodies, Monoclonal; D001886:Bordetella pertussis / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012703:Serotyping / Q000379:methods; D013548:Sweden; D014917:Whooping Cough / Q000175:diagnosis
D000911:Antibodies, Monoclonal