title: Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma.
abstract: A bioanalytical method for the determination of lumefantrine (LF) and its metabolite desbutyl-lumefantrine (DLF) in plasma by solid-phase extraction (SPE) and liquid chromatography has been developed. Plasma proteins were precipitated with acetonitrile:acetic acid (99:1, v/v) containing a DLF analogue internal standard before being loaded onto a octylsilica (3 M Empore) SPE column. Two different DLF analogues were evaluated as internal standards. The compounds were analysed by liquid chromatography UV detection on a SB-CN (250 mm x 4.6 mm) column with a mobile phase containing acetonitrile-sodium phosphate buffer pH (2.0; 0.1 M) (55:45, v/v) and sodium perchlorate 0.05 M. Different SPE columns were evaluated during method development to optimise reproducibility and recovery for LF, DLF and the two different DLF analogues. The within-day precisions for LF were 6.6 and 2.1% at 0.042 and 8.02 microg/mL, respectively, and for DLF 4.5 and 1.5% at 0.039 and 0.777 microg/mL, respectively. The between-day precisions for LF were 12.0 and 2.9% at 0.042 and 8.02 microg/mL, respectively, while for DLF 0.7 and 1.2% at 0.039 and 0.777 microg/mL, respectively. The limit of quantification was 0.024 and 0.021 microg/mL for LF and DLF, respectively. Different amounts of lipids in plasma did not affect the absolute recovery of LF or DLF.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. niklas@tropmedres.ac
authors.forename: N
authors.initials: N
authors.lastname: Lindegårdh
authors.forename: A
authors.initials: A
authors.lastname: Annerberg
authors.forename: D
authors.initials: D
authors.lastname: Blessborn
authors.forename: Y
authors.initials: Y
authors.lastname: Bergqvist
authors.forename: N
authors.initials: N
authors.lastname: Day
authors.forename: N J
authors.initials: NJ
authors.lastname: White
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D004983:Ethanolamines / Q000097:blood* / Q000378:metabolism; D005449:Fluorenes / Q000097:blood* / Q000378:metabolism; D006801:Humans; D000078102:Lumefantrine; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D004983:Ethanolamines; D005449:Fluorenes; D000078102:Lumefantrine