title: Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization.
abstract: Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 mug of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future.
authors.affiliation: Institute of Feed Science, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No. 164 Qiutao North Road, Hangzhou 310029, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wu
authors.forename: Guangfu
authors.initials: G
authors.lastname: Liu
authors.forename: Cuiping
authors.initials: C
authors.lastname: Cao
authors.forename: Haiqing
authors.initials: H
authors.lastname: Huang
authors.forename: Zirong
authors.initials: Z
authors.lastname: Xu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D012831:Bombyx / Q000821:virology*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects; D007218:Industrial Microbiology / Q000379:methods; D007781:Lactoferrin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018411:Spodoptera / Q000821:virology; D013552:Swine
chemical_list: D011994:Recombinant Proteins; D007781:Lactoferrin