[Pharmacokinetics and distribution of superoxide dismutase liposomes in rats].
OBJECTIVE
To evaluate the effects of surfactants on the pharmacokinetics and distribution in rats after intravenous administration of SOD liposomes.


METHODS
The liposomes were prepared by reverse phase evaporation method. The activity of SOD was assayed by method of xanthine oxidase.


RESULTS
The T1/2 of SOD solution, common SOD liposome, SOD liposomes modified by DSPE-PEG2000 and Tween 80 were 0.25, 0.34, 0.66 and 0.41 h, respectively; AUC were 12.48, 24.66, 41.16 and 33.02 microg x h x mL(-1), respectively. Compared with the common liposome, the liposomes modified by DSPE-PEG and Tween 80 decreased the content of SOD in liver and spleen, but increased in brain.


CONCLUSIONS
The three kinds of liposomes could increase T1/2 and AUC in some extent, especially in PEG-L group. Tween-L could increase the SOD content in brain, and PEG-L could decrease the SOD content in the liver and spleen compared with the common liposome.
Zhang
Yun-long
YL
Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Xie
Ying
Y
Wang
Hui-juan
HJ
Hou
Xin-pu
XP
Liu
Yan
Y
D000818:Animals; D019540:Area Under Curve; D001921:Brain / Q000201:enzymology*; D007275:Injections, Intravenous; D008081:Liposomes; D008099:Liver / Q000201:enzymology; D008297:Male; D011136:Polysorbates / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000201:enzymology; D013482:Superoxide Dismutase / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D013501:Surface-Active Agents / Q000494:pharmacology; D014018:Tissue Distribution
D008081:Liposomes; D011136:Polysorbates; D013501:Surface-Active Agents; D013482:Superoxide Dismutase