Study on the resistance of severe acute respiratory syndrome-associated coronavirus.
In this study, the persistence of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) was observed in feces, urine and water. In addition, the inactivation of SARS-CoV in wastewater with sodium hypochlorite and chlorine dioxide was also studied. In vitro experiments demonstrated that the virus could only persist for 2 days in hospital wastewater, domestic sewage and dechlorinated tap water, while 3 days in feces, 14 days in PBS and 17 days in urine at 20 degrees C. However, at 4 degrees C, the SARS-CoV could persist for 14 days in wastewater and at least 17 days in feces or urine. SARS-CoV is more susceptible to disinfectants than Escherichia coli and f2 phage. Free chlorine was found to inactivate SARS-CoV better than chlorine dioxide. Free residue chlorine over 0.5 mg/L for chlorine or 2.19 mg/L for chlorine dioxide in wastewater ensures complete inactivation of SARS-CoV while it does not inactivate completely E. coli and f2 phage.
Wang
Xin-Wei
XW
Tianjin Institute of Environment and Health, 1 Da Li Road, Tianjin 300050, PR China.
Li
Jin-Song
JS
Jin
Min
M
Zhen
Bei
B
Kong
Qing-Xin
QX
Song
Nong
N
Xiao
Wen-Jun
WJ
Yin
Jing
J
Wei
Wei
W
Wang
Gui-Jie
GJ
Si
Bing-Yin
BY
Guo
Bao-Zhong
BZ
Liu
Chao
C
Ou
Guo-Rong
GR
Wang
Min-Nian
MN
Fang
Tong-Yu
TY
Chao
Fu-Huan
FH
Li
Jun-Wen
JW
D002713:Chlorine / Q000494:pharmacology; D017606:Chlorine Compounds / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005243:Feces / Q000821:virology; D006801:Humans; D017909:Levivirus / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012722:Sewage / Q000821:virology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014556:Urine / Q000821:virology; D038301:Virus Inactivation; D014871:Water Microbiology
D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; D012367:RNA, Viral; D012722:Sewage; D002713:Chlorine; C025109:chlorine dioxide; D012973:Sodium Hypochlorite