[The unbalance of membrane phospholipid composition of Escherichia coli affects the PPHO promoter activity].
Activity of phosphate PPHO or arabinose PBAD promoter of Escherichia coli has been studied depending on the content of zwitter-ionic phosphatidylethanolamine (PE) and anionic phospholipids in membranes. In the absence of PE or under significant decrease in the content of anionic phospholipids, there is a significant decline of PPHO promoter activity but not PBAD promoter. Since the PPHO promoter belongs to the Pho-regulon--a member of the family of two-component regulatory systems of signal transduction having membrane sensors, the regulation of gene expression by phospholipids is presumed to be realized through a membrane sensor.
Golovastov
V V
VV
Krasovskaia
L A
LA
Anisimova
E V
EV
Shukin
V N
VN
Badiakina
A O
AO
Nesmeianova
M A
MA
D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015398:Signal Transduction
D004269:DNA, Bacterial; D008563:Membrane Lipids; D010743:Phospholipids