title: The coinitiator DMABEE induces death by apoptosis and necrosis in human monoblastoid cells.
abstract: 4-N,N-Dimethyl amino benzoic acid ethylester (DMABEE), a leachable lipophilic component of polymer-based dental-filling materials, has been shown to interact with cell membrane phospholipids, such as phosphatidylcholine and phosphatidylserine (PS). One marker of cellular death by apoptosis is the change in architecture of the plasma membrane involving the translocation of the negatively charged PS from the inner to the outer leaflet of the cell membrane. We therefore hypothesized that DMABEE has the potential to induce apoptosis. The necrosis inducing potential was also investigated. To test our hypothesis human monoblastoid U-937 cells were exposed to 10, 20, 40, 80, 120, 160, and 200 microM of DMABEE for 24, 48, and 72 h. At the culture end-points apoptotic and necrotic cells were detected by flow cytometry. DMABEE enhanced cell death by apoptosis and necrosis in U-937 cells in a dose-dependent fashion. The data support our hypothesis that DMABEE triggers death-signaling pathways.
authors.affiliation: Department of Dental Biomaterials, Institute of Odontology, Faculty of Dentistry, University of Bergen, Aarstadveien 17, 5009 Bergen, Norway. Mihaela-Roxana.Cimpan@cih.uib.no
authors.forename: Mihaela Roxana
authors.initials: MR
authors.lastname: Cimpan
authors.forename: Roald
authors.initials: R
authors.lastname: Matre
authors.forename: Nils
authors.initials: N
authors.lastname: Skaug
authors.forename: Stein Atle
authors.initials: SA
authors.lastname: Lie
authors.forename: Henning
authors.initials: H
authors.lastname: Lygre
mesh_terms: D010129:4-Aminobenzoic Acid / Q000633:toxicity; D000704:Analysis of Variance; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003188:Composite Resins / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects*; D009336:Necrosis / Q000139:chemically induced; D044367:Phase Transition; D020298:U937 Cells / Q000187:drug effects; D062366:para-Aminobenzoates
chemical_list: D017304:Annexin A5; D003188:Composite Resins; D008563:Membrane Lipids; D062366:para-Aminobenzoates; C030747:ethyl 4-dimethylaminobenzoate; D010129:4-Aminobenzoic Acid