[Effects of selenoprotein on blood glucose, Ca2+ transfer and NO system in diabetic mice].
OBJECTIVE
To study the controlling effect of selenoprotein on blood glucose, Ca2+ transfer and NO system in diabetic mice.


METHODS
Male Kunming mice of (20.3 +/- 1.7) g body weight were injected 200 mg/kg bw, 2% alloxan in abdomen to make diabetic model (DM), and were randomly divided into six groups: normal control group (I), normal + selenoprotein group(Se 100 microg/kg bw) (lI), DM control group (III), DM + lower dose selenoprotein group(Se 100 microg/kg bw) (IV), DM + higher dose selenoprotein group (Se 300 microg/kg bw) (V), DM + Na2SeO3 group (Se 100 microg/kg bw) (VI).


RESULTS
The level of blood glucose in V group [(20.4 +/- 6.3 mmol/L] were significantly lower than lII group(45.3 +/- 3.3) mmol/L P < 0.05. The activity of Ca(2+) -ATPase of kidney in V group (0.90 +/- 0.5 micromol/ (h x mg prot) is significantly higher than III group [(0.35 +/- 0.1) micromol/(h x mg prot)] (P < 0.05, and the activity of NOS in V group [(25.0 +/- 4.3) U/ml] is significantly lower than III group [(35.2 +/- 4.4) U/ml] (P < 0.05).


CONCLUSIONS
Selenoprotein that supplemented selenium doseis 300 microg Se/kg weight could significantly decrease blood glucose, increase the activities of Ca(2+) -ATPase on kidney and weaken the activities of plasma NOS in diabetic mice.
Luo
Jun
J
Hebei Center of Prevent and Control Disease, Shijiazhuang 050021, China.
Zhang
Shi-Lian
SL
Wang
Wei
W
Bai
Ping
P
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D051379:Mice; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011897:Random Allocation; D051140:Selenoproteins / Q000494:pharmacology*
D001786:Blood Glucose; D051140:Selenoproteins; D019001:Nitric Oxide Synthase; D000252:Calcium-Transporting ATPases