Tissue distribution, subcellular localization and endocrine disruption patterns induced by Cr and Mn in the crab Ucides cordatus.
The essential trace elements Cr and Mn are toxic at high concentrations and information about low concentration is insufficient in the literature. In polluted mangroves, the crab Ucides cordatus can represent a useful tool to assess information on the potential impact of trace elements like Cr and Mn on the environment, since this species is comestible and thus, commercially negotiated. Therefore, U. cordatus crabs were exposed in vivo to different concentrations of Cr and Mn solved in seawater and had their tissue distribution and subcellular deposits evaluated. The gill, hepatopancreas and muscle concentrations were determined by atomic absorption spectroscopy and the results showed that Cr and Mn presented the highest values in the gills rather than in the hepatopancreas and muscular tissue. Electron microscopy and analytical X-ray microanalysis revealed Cr precipitates on the gill surface, co-localized with epiphyte bacteria. In addition, since Cr and Mn did not equally accumulate in most of the tissues studied, glycemic rate of animals, which received injections of extracts of eyestalks of the contaminated crabs, were measured in order to evaluate whether the studied concentrations of Cr and Mn could produce any metabolic alteration. The results indicated that extracts of the eyestalks of crabs submitted to Cr and Mn salts and injected into normal crabs markedly influenced crustacean hyperglycemic hormone synthesis and/or release. The results are discussed with respect to sensitivity of the employed methods and the possible significance of the concentrations of Cr and Mn in the organisms.
Corrêa
José Dias
JD
Programa de Pós-Graduação em Ciências Morfológicas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil. correajr@anato.ufrj.br
da Silva
Miguel Ramos
MR
da Silva
Antonio Carlos Bastos
AC
de Lima
Silene Maria Araújo
SM
Malm
Olaf
O
Allodi
Silvana
S
D000704:Analysis of Variance; D000818:Animals; D060829:Arthropod Proteins; D003386:Brachyura / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D004703:Endocrine System / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000378:metabolism / Q000648:ultrastructure; D043143:Hepatopancreas / Q000378:metabolism / Q000648:ultrastructure; D007447:Invertebrate Hormones; D008345:Manganese / Q000378:metabolism / Q000493:pharmacokinetics*; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D012623:Seawater; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity
D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D014874:Water Pollutants, Chemical; C015947:hyperglycemic hormone, crustacean; D002857:Chromium; D008345:Manganese