Ethylene glycol induces hyperoxaluria without metabolic acidosis in rats.
Ethylene glycol (EG) consumption is commonly employed as an experimental regimen to induce hyperoxaluria in animal models of calcium oxalate nephrolithiasis. This approach has, however, been criticized because EG overdose induces metabolic acidosis in humans. We tested the hypothesis that EG consumption (0.75% in drinking water for 4 wk) induces metabolic acidosis by comparing arterial blood gases, serum electrolytes, and urinary chemistries in five groups of Sprague-Dawley rats: normal controls (CON), those made hyperoxaluric (HYP) with EG administration, unilaterally nephrectomized controls (UNI), unilaterally nephrectomized rats fed EG (HRF), and a metabolic acidosis (MA) reference group imbibing sweetened drinking water (5% sucrose) containing 0.28 M NH4Cl. Arterial pH, plasma bicarbonate concentrations, anion gap, urinary pH, and the excretion of titratable acid, ammonium, phosphate, citrate, and calcium in HYP rats were not significantly different from CON rats, indicating that metabolic acidosis did not develop in HYP rats with two kidneys. Unilateral nephrectomy alone (UNI group) did not significantly affect arterial pH, plasma bicarbonate, anion gap, or urinary pH compared with CON rats; however, HRF rats exhibited some signs of a nascent acidosis in having an elevated anion gap, higher phosphate excretion, lower urinary pH, and an increase in titratable acid. Frank metabolic acidosis was observed in the MA rats: decreased arterial pH and plasma HCO3(-) concentration with lower urinary pH and citrate excretion with elevated excretion of ammonium, phosphate and, hence, titratable acid. We conclude that metabolic acidosis does not develop in conventional EG treatments but may ensue with renal insufficiency resulting from an oxalate load.
Green
Mike L
ML
Dept. of Pathology, Immunology, and Laboratory Medicine, Univ. of Florida, College of Medicine, PO Box 100275, Gainesville, FL 32610-00275, USA. greenmj@pathology.ufl.edu
Hatch
Marguerite
M
Freel
Robert W
RW
D000138:Acidosis; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D004195:Disease Models, Animal; D004573:Electrolytes / Q000097:blood / Q000652:urine; D019855:Ethylene Glycol / Q000494:pharmacology*; D006959:Hyperoxaluria / Q000139:chemically induced* / Q000378:metabolism*; D007669:Kidney Calculi / Q000139:chemically induced / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000139:chemically induced / Q000378:metabolism; D007677:Kidney Function Tests; D008297:Male; D010070:Oxalates / Q000652:urine; D010100:Oxygen / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
D004573:Electrolytes; D010070:Oxalates; D002245:Carbon Dioxide; D019855:Ethylene Glycol; D010100:Oxygen