Aerobic exercise training decreases leucine oxidation at rest in healthy adults.
Both exercise and dietary protein intake affect whole-body protein turnover (WBPTO). Few studies have investigated the effect of aerobic exercise training on WBPTO [leucine rate of appearance (Ra), oxidation (Ox), and nonoxidative leucine disposal (NOLD)] in untrained individuals consuming a specified level of protein. This study examined the effect of aerobic exercise training on WBPTO in untrained men and women during a controlled diet intervention providing 0.88 g protein/(kg . d). After a 2-wk adaptation to the study diet, 7 subjects [3 men, 4 women; 76.1 +/- 5.8 kg, 164.7 +/- 4.4 cm, 30.7 +/- 4.5% body fat, 39.1 +/- 2.8 VO(2max) (maximal oxygen uptake) mL/(kg . min)] participated in 4 wk of aerobic exercise training (running and walking 4-5 times/wk at 65-85% maximal heart rate). WBPTO (determined via constant infusion of 1-[(13)C] leucine), nitrogen balance, and body composition were determined at baseline and after 4 wk of training. Nitrogen balance (-1.0 +/- 0.7 vs. 0.9 +/- 1.1 g N/24 h, P = 0.03) improved with exercise training, whereas body mass and composition did not change. Leucine Ra did not change, Ox decreased [18 +/- 2 to 15 +/- 2 micromol/(kg . h), P </= 0.001], and NOLD tended to increase [128 +/- 18 to 151 +/- 19 micromol/(kg . h), P = 0.09] in response to training. These data indicate improved protein utilization in response to exercise training in weight-stable subjects. This study emphasizes the importance of dietary control, with specific regard to energy and protein intakes, in the characterization of protein utilization in response to an exercise intervention.
Gaine
Patricia C
PC
Department of Nutritional Sciences, University of Connecticut, Storrs 06269, USA.
Viesselman
Christian T
CT
Pikosky
Matthew A
MA
Martin
William F
WF
Armstrong
Lawrence E
LE
Pescatello
Linda S
LS
Rodriguez
Nancy R
NR
D000328:Adult; D001481:Basal Metabolism; D001835:Body Weight; D004032:Diet; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007930:Leucine / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010809:Physical Fitness; D012016:Reference Values; D012146:Rest / Q000502:physiology*
D007930:Leucine