Inhibition of tumor growth by plant-derived mAb.
The tumor-associated antigen EpCAM (GA733-2) is a highly expressed target on adenocarcinoma cells, as defined by murine mAb CO17-1A. We recently developed a transgenic plant system for the safe and inexpensive production of large quantities of mAb CO17-1A as a future source of clinical-grade protein. Although the glycosylation pattern of plant-derived mAb (mAb(P)) CO17-1A differs considerably from that of the mammalian-derived mAb (mAb(M)), we show here that the biological activity of both mAbs is quite similar. mAb(P) heavy and light chains assembled to bind the recombinant antigen GA733-2E and specifically bound to human SW948 colorectal carcinoma cells expressing the antigen GA733-2 to the same extent as mAb(M). mAb(P) was as effective as mAb(M) CO17-1A in inhibiting tumor growth of xenotransplanted SW948 cells in nude mice. These results suggest the promise of transgenic plants as a useful alternative way to produce full-size mAb for cancer immunotherapy.
Ko
Kisung
K
Biotechnology Foundation Laboratories, Thomas Jefferson University, 1020 Locust Street, M-85 Jefferson Alumni Hall, Philadelphia, PA 19107-6799, USA.
Steplewski
Zenon
Z
Glogowska
Magdalena
M
Koprowski
Hilary
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism; D000912:Antibodies, Neoplasm / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000737:chemistry; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D007167:Immunotherapy / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008957:Models, Genetic; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000628:therapy*; D010944:Plants / Q000276:immunology* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D011994:Recombinant Proteins