Prostaglandin E1-initiated immune regulation during human mixed lymphocyte reaction.
Prostaglandin E1 (PGE1) has therapeutic value for transplantations due to its microvascular activity. Interleukin (IL)-18, which is elevated in plasma during the acute rejection after organ transplantation, elicits the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40, and CD40 ligand (CD40L) on monocytes as well as the production of interferon (IFN)-gamma and IL-12 and proliferation of T-cells during the human mixed lymphocyte reaction (MLR) in an in vitro model of acute rejection. In contrast, PGE1 inhibits all the adhesion molecule expression, cytokine production and T-cell proliferation in the presence of IL-18. The effects of PGE1 depend on stimulation of the IP/EP2/EP4-receptor, and thus, PGE1 might have therapeutic potential for treating acute rejection due to its immune regulatory effect.
Takahashi
Hideo K
HK
Department of Pharmacology, Okayama University Graduate School of Medicine and Dentistry, Okayama City 700-8558, Japan.
Xue
Dong
D
Iwagaki
Hiromi
H
Tamura
Ryuji
R
Katsuno
Goutarou
G
Yagi
Takahito
T
Yoshino
Tadashi
T
Mori
Shuji
S
Nishibori
Masahiro
M
Tanaka
Noriaki
N
D000527:Alprostadil / Q000494:pharmacology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000096:biosynthesis / Q000276:immunology; D023201:CD40 Ligand / Q000096:biosynthesis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D020382:Interleukin-18 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D015703:Antigens, CD; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; D020382:Interleukin-18; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1; D023201:CD40 Ligand; D007371:Interferon-gamma; D000527:Alprostadil