Characterization and expression analysis of the chicken interleukin-11 receptor alpha chain.
Interleukin-11 (IL-11) is a multifunctional cytokine involved in various pathways in blood cells, their precursors and many other cell types in vitro and in vivo. The effects of IL-11 are largely mediated by the IL-11 receptor alpha-chain (IL-11Ralpha). In this study, a putative cDNA sequence encoding the 414 amino acid propeptide of chicken IL-11R (chIL-11R) was identified. The predicted 414 amino acid sequence showed 42-43% sequence identity with mammalian homologues. In a domain search of the molecule, two fibronectin (FN) type-III domains were identified in the C- terminal portion. On comparison with mammalian IL-11R, 4 conserved cysteine residues and a WSXWS motif were observed within the FN type-III domains. Expression analysis revealed that chIL-11Ralpha is strongly expressed in brain, heart, lung, liver, glandular stomach, kidney, the immature testis, ovary and chicken blastodermal cells (CBCs) after 1-day-cultivation. These findings strongly indicate that the identified chicken cDNA sequence encodes chIL-11R alpha-chain homologue.
Kawashima
Tsuyoshi
T
Hiroshima Prefectural Institute of Industrial Science and Technology, Higashi-Hiroshima, Hirohima 739-0046, Japan.
Hojyo
Shintaro
S
Nishimichi
Norihisa
N
Sato
Masaharu
M
Aosasa
Masayoshi
M
Horiuchi
Hiroyuki
H
Furusawa
Shuichi
S
Matsuda
Haruo
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics*; D053702:Receptors, Interleukin-11
D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11