title: Characterization of different cell culture media for expression of recombinant antibodies in mammalian cells: Presence of contaminating bovine antibodies.
abstract: Several new cell culture media designed specifically for the expression of recombinant antibodies in Chinese hamster ovary (CHO) cells were investigated for the presence of bovine IgG. Three serum-free media, three protein-free (animal component free) media, as well as one chemically defined medium were included in the study. Employing a combination of affinity chromatography (Protein G or A columns), SDS-PAGE analysis, and peptide mass fingerprinting, two of the serum-free media were found to contain bovine IgG in the range of approximately 0.5 mg/L. The other five media did not contain detectable levels of contaminating Protein A or G-binding proteins such as bovine IgG.
authors.affiliation: Symphogen A/S, DK-2800 Lyngby, Denmark.
authors.forename: Lone Kjaer
authors.initials: LK
authors.lastname: Rasmussen
authors.forename: Yvonne Berger
authors.initials: YB
authors.lastname: Larsen
authors.forename: Peter
authors.initials: P
authors.lastname: Højrup
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000737:chemistry; D016895:Culture Media, Serum-Free / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects
chemical_list: D000906:Antibodies; D003470:Culture Media; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D007074:Immunoglobulin G; D011994:Recombinant Proteins