A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection.
The adenovirus IVa2 is an intermediate viral gene product that appears to perform multiple essential roles in viral infection. Using IVa2 as bait in the yeast two-hybrid system, we screened selected open reading frames (ORFs) of porcine adenovirus (PAdV)-3 for potential interaction with IVa2. Interestingly, pVIII showed specific interaction with IVa2. The yeast two-hybrid findings were validated by GST pull-down assays, in vitro binding studies employing cell-free coupled transcription-translation products and in vitro co-immunoprecipitations using protein-specific antibodies. Finally, we demonstrated that IVa2 specifically interacts with pVIII during PAdV-3 infection.
Singh
Mahavir
M
Vaccine and Infectious Disease Organization, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
Shmulevitz
Maya
M
Tikoo
Suresh Kumar
SK
D025662:Adenoviruses, Porcine / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D011485:Protein Binding; D013552:Swine; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins; C056232:iva2 protein, adenovirus