Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4.
Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonas paucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2,3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type metapathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas.
Xia
Ying
Y
College of Life Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China.
Min
Hang
H
Rao
Gang
G
Lv
Zhen-mei
ZM
Liu
Ji
J
Ye
Yang-fang
YF
Duan
Xue-jun
XJ
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010616:Phenanthrenes; D011506:Proteins; D012989:Soil Pollutants; C031181:phenanthrene; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D005982:Glutathione Transferase; C092541:2-hydroxymuconate-semialdehyde hydrolase