title: In vitro detection and characterisation of a polyphosphate synthesising activity in the yeast Candida humicola G-1.
abstract: An in vitro detectable polyphosphate-synthesising activity was characterised using two independent assay systems in extracts of the yeast Candida humicola G-1. Its properties were similar to those of a range of bacterial polyphosphate kinase enzymes. PCR amplification of C. humicola genomic DNA using universal primers for bacterial polyphosphate kinase genes yielded a product whose translated sequence showed up to 34% amino acid similarity to the bacterial enzyme.
authors.affiliation: School of Biology and Biochemistry and QUESTOR Centre, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Rd., Belfast, BT9 7BL, Northern Ireland. j.mcgrath@qub.ac.uk
authors.forename: John W
authors.initials: JW
authors.lastname: McGrath
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kulakova
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Kulakov
authors.forename: John P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000378:metabolism*; D016415:Sequence Alignment; D013696:Temperature
chemical_list: D003470:Culture Media; D011122:Polyphosphates; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase