title: The change of HSP47, collagen specific molecular chaperone, expression in rat skeletal muscle may regulate collagen production with gravitational conditions.
abstract: It is well known that unloading of skeletal muscle with spaceflight leads skeletal muscle atrophy. However, it remains unclear how the extracellular matrix within the muscle and the connective tissues such as tendon and ligament respond to reduced mechanical load including microgravity, although they have been thought to play important roles in both the transmission of force and the signal transduction between cells and tissues. Type-I collagen and type-IV collagen, both of the major components of extracellular matrix and connective tissues. We focused on change of these collagen synthesis with mechanical load. To obtain an insight into the effects of gravitational changing on the protein metabolism of collagen in skeletal muscle during mechanical unloading, reloading after unloading, we investigated changes in the amount of Heat shock protein 47 (HSP47), has been postulated to be a collagen-specific molecular chaperone localized in the ER (Nagata et al, 1992). Western blot analysis revealed that HSP47 in rat soleus muscle decreases at 5 days after hindlimb suspension (HS). On the other hand, HSP47 in rat soleus muscle increases at 5 days after hypergravity (HG) induced by the centrifugation. RT-PCR analysis showed HSP47 mRNA decreased with HS earlier, as compared with collagen type-I and type-IV mRNA. From these results, the amount of HSP47 changing by gravitational condition may effect on signal transfers in the primary stage of adaptation and the change of HSP47 expression in skeletal muscle may regulate collagen production with gravitational conditions.
authors.affiliation: Department of Life Sciences, The Graduate School of Arts & Sciences, The University of Tokyo.
authors.forename: Asami
authors.initials: A
authors.lastname: Oguro
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakurai
authors.forename: Makoto
authors.initials: M
authors.lastname: Okuno
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nagata
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Atomi
mesh_terms: D000818:Animals; D002498:Centrifugation; D024042:Collagen Type I / Q000096:biosynthesis; D024141:Collagen Type IV / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000502:physiology; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D019417:Hindlimb Suspension; D018471:Hypergravity; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015843:Serpins / Q000096:biosynthesis* / Q000378:metabolism; D013314:Stress, Mechanical; D018474:Weightlessness Simulation
chemical_list: D024042:Collagen Type I; D024141:Collagen Type IV; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; C494662:Serpinh1 protein, rat; D015843:Serpins