[Angiogenic factors in external auditory canal cholesteatoma-fibroblast cell culture].
BACKGROUND
The external auditory canal cholesteatoma (EACC) is characterized by hyperproliferation of the epithelial and subepithelial tissue. Compared to normal meatal skin, strong expression of FGF-2 and VEGF had previously been detected. Many authors reported that FGF-2 acts via VEGF and is induced by hypoxia. Hypoxia seems to be pivotal for establishing EACC. Therefore, human EACC fibroblasts were investigated by incubating with FGF-2 and determining VEGF.


METHODS
We harvested fibroblasts from human EACC and normal meatal skin and incubated the fibroblast culture with 50 ng/ml FGF-2 and determined VEGF concentrations after 1-4 days.


RESULTS
Compared to untreated fibroblast cultures, there was a significant increase of VEGF concentration (p<0.05). However, there was no significant difference between the proliferation quantities.


CONCLUSIONS
VEGF and FGF-2 are possibly involved in a cascade of growth factor activities, which modulates their concentration in human-derived EACC fibroblast culture. Exogenous FGF-2 increased fibroblast expression for VEGF, which is a major autocrine mediator of FGF-2-induced angiogenesis and proliferation.
Naim
R
R
Universitäts-Hals-Nasen-Ohren-Klinik, Mannheim. ramin.naim@hno.ma.uni-heidelberg.de
Sadick
H
H
Bayerl
C
C
Bran
G
G
Hörmann
K
K
D043925:Angiogenesis Inducing Agents / Q000032:analysis*; D001706:Biopsy; D002455:Cell Division / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D018424:Cholesteatoma, Middle Ear / Q000473:pathology*; D004424:Ear Canal / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D043925:Angiogenesis Inducing Agents; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2