[Effects of lingfasu on embryoid and plantlet formation of Panax notoginseng in vitro].
On the medium MS added the right amount of 2,4-D and LFS (Angustmycin) and cultured under dark condition, the callus from stem segments of Panax notoginseng could induce a lot of embryoids. In 2-3 months, the ratio of embryoid formation reached about 90%. Then transplanted on MS + 2,4-D 1.5 mg/L + LFS 2 mg/L and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets.
Xu
Hongyuan
H
Agricultural College, Guangxi University, Nanning 530005.
Meng
Aidong
A
Li
Chunxia
C
Deng
Xiqing
X
Zhou
Qiwei
Q
Yang
Meichun
M
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media; D005894:Panax / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators; C012182:angustmycin A; D000241:Adenosine