title: [Content analysis of bilirubin in niuhuang compound suppository by HPLC].
abstract: OBJECTIVE
  To establish a method of determinating content of bilirubin in Niuhuang Compound Suppository by HPLC.
  
  
  METHODS
  C18 column was used. The mobile phase was consisted of dimethylsulfoxide: acetonitrile: 0.5% acetic ammonium (1:1.4:1). Detector wavelength was 456 nm. The extraction solution for bilirubin was consisted of dlimethylsulfoxide: acetonitrile = 9:4.
  
  
  RESULTS
  The linearity was obtained over the range of 0.107 - 2.14 microg. The mean recovery was 101.0%, RSD = 0.518% (n = 5).
  
  
  CONCLUSIONS
  The method was sensitive, simple and accurate.
authors.affiliation: Hubei College of Traditional Chinese Medicine, Wuhan 430061.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Guoping
authors.initials: G
authors.lastname: Gan
authors.forename: Zai'an
authors.initials: Z
authors.lastname: He
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Liu
authors.forename: Keli
authors.initials: K
authors.lastname: Shi
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D008419:Materia Medica / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013488:Suppositories; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D013488:Suppositories; D001663:Bilirubin