title: Comparative molecular analysis of Haemophilus influenzae isolates from young children with acute lower respiratory tract infections and meningitis in Hanoi, Vietnam.
abstract: Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no beta-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns alpha (22 isolates) and beta (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam.
authors.affiliation: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. h-wata@net.nagasaki-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kaji
authors.forename: Dang Duc
authors.initials: DD
authors.lastname: Anh
authors.forename: Phan Le Thanh
authors.initials: Ple T
authors.lastname: Huong
authors.forename: Nguyen Thi Hien
authors.initials: NT
authors.lastname: Anh
authors.forename: Vu Thi
authors.initials: VT
authors.lastname: Huong
authors.forename: Hoang Vu Mai
authors.initials: HV
authors.lastname: Phuong
authors.forename: Ngo Thi
authors.initials: NT
authors.lastname: Thi
authors.forename: Pham Thi
authors.initials: PT
authors.lastname: Suu
authors.forename: Nguyen Thi Thu
authors.initials: NT
authors.lastname: Nguyet
authors.forename: Olivia Sebastian
authors.initials: OS
authors.lastname: Rusizoka
authors.forename: Kiwao
authors.initials: K
authors.lastname: Watanabe
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nagatake
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006192:Haemophilus Infections / Q000175:diagnosis*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D016920:Meningitis, Bacterial / Q000175:diagnosis*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D015183:Restriction Mapping
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial