title: Modulation of cell mutability by serum factors from volunteers who experienced head-down-tilt bed rest.
abstract: Head-down-tilt bed rest (BR) is utilized to mimic a microgravity environment. In this study, we examined whether the modulating activity of serum factors on cell mutability was changed in seventeen volunteers subjected to BR. For comparison, serum was comparatively obtained from seven volunteers who experienced the parabolic flight (PF). Cultured human cells were treated with serum and then irradiated with UV or X-ray. Mutagenicity was estimated by identification of K-ras codon 12 base substitution mutation, following PCR and differential dot-blot hybridization. Modulating activity was detected in one sample from each of post-BR and post-PF serum.
authors.affiliation: Graduate School of Medicine, Chiba University, Chuo-ku, Chiba.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugaya
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kita
authors.forename: Hideoki
authors.initials: H
authors.lastname: Fukuoka
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D001510:Bed Rest; D002460:Cell Line; D002478:Cells, Cultured; D003062:Codon; D011905:Genes, ras / Q000235:genetics* / Q000528:radiation effects; D018475:Head-Down Tilt; D006801:Humans; D009154:Mutation; D013026:Space Flight; D014466:Ultraviolet Rays; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: D003062:Codon