title: The relationship between O-antigens and pathogenic genes of diarrhea-associated Escherichia coli.
abstract: To evaluate the serogrouping-based diagnosis of diarrheagenic Escherichia coli, a total of 1,130 strains of E. coli isolated in several countries were studied. The strains were regarded as enterovirulent on the basis of their O-antigens determined using a commercially available kit containing 43 antisera, and the presence of diarrhea-associated genes (eae, stx, aggR, est, elt, ipaH) was evaluated by PCR. Two hundred sixty-three strains of 1,130 (23.3%) were identified as diarrheagenic based on the presence of at least one pathogenic gene. The probability that E. coli identified as diarrheagenic on the basis of serogrouping actually possessed some pathogenic gene was highest for serogroup O119 (78.4%); other serogroups with a positive rate for pathogenic genes higher than 60% were O111 and O126. No target genes were detected among the strains belonging to serogroups O1, O29, O112ac, O143, O158 and O168. Our results suggest that, in practice, serogrouping is useful for the identification of diarrheagenic E. coli in a very limited number of serogroups.
authors.affiliation: Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan. k028762@med.u-ryukyu.ac.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Miyazato
authors.forename: Noboru
authors.initials: N
authors.lastname: Nakasone
authors.forename: Naomi
authors.initials: N
authors.lastname: Higa
authors.forename: Claudia
authors.initials: C
authors.lastname: Toma
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iwanaga
mesh_terms: D000942:Antigens, Bacterial; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D005243:Feces / Q000382:microbiology; D006801:Humans; D019081:O Antigens / Q000502:physiology*; D011933:Reagent Kits, Diagnostic; D012703:Serotyping; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D019081:O Antigens; D011933:Reagent Kits, Diagnostic