Simultaneous determination of unmodified sevoflurane and of its metabolite hexafluoroisopropanol in urine by headspace sorptive extraction-thermal desorption-capillary gas chromatography-mass spectrometry.
Unmodified sevoflurane and its metabolite, hexafluoroisopropanol (HFIP), have both been proposed as biomarkers of exposure in post-shift urine for operating room personnel exposed to inhalation anaesthetic sevoflurane. We used headspace sorptive extraction (HSSE) and thermal desorption-capillary GC-MS to assess sensitively both compounds in the urine matrix (after a HFIP deconjugation step). In GC-MS splitless mode, calibration plots (approximately 15-650 microg/L) were linear (r2 > 0.9910) and the limits of detection (1 microg/L for both biomarkers) showed increased sensitivity for HFIP with respect to the previously described headspace GC-MS method. The method was suitable for biological monitoring of both biomarkers of exposure to sevoflurane.
Accorsi
Antonio
A
Safety, Hygiene and Occupational Medicine Service, University of Bologna, Via Pelagio Palagi 9, Bologna 40138, Italy. antonio.accorsi@unibo.it
Morrone
Barbara
B
Benzo
Maurizio
M
Gandini
Carlo
C
Raffi
Giovanni Battista
GB
Violante
Francesco Saverio
FS
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008738:Methyl Ethers / Q000652:urine*; D020005:Propanols / Q000652:urine*; D012680:Sensitivity and Specificity; D000077149:Sevoflurane; D006845:Hydrocarbons, Fluorinated
D008738:Methyl Ethers; D020005:Propanols; D000077149:Sevoflurane; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated