[Effect of sulphamethazine on the gene expression of FRTL-5 cells].
OBJECTIVE
To study the effect of Sulphamethazine on the gene expression of FRTL-5 cells, and to explore the mechaniams of environmental thyroid hormone disruptors.


METHODS
cDNA microarray technique was used to analyze the gene expression of FRTL-5 cells of exponential phase treated by 2.0 microg/ml Sulphamethazine for 24 h. Total RNA from treated and untreated cells were labeled by Cy3 dCTP and Cy5 dCTP respectively. The ratios of Cy3/Cy5 were calculated in order to find the genes which expressed differently.


RESULTS
There were 679 genes (approximately 7%, total 9753 genes) exhibiting different expression, in which 395 (4.0%) genes up-regulated and 284 (3.0%) genes down-regulated. These genes related to regulation of gene expression, regulation of cell cycle, metabolism in cells, and so on.


CONCLUSIONS
The effect of Sulphamethazine on FRTL-5 cells may be related with a series of genes.
Wang
Jin-Tao
JT
West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sun
Ding
D
Pan
Hong-Mei
HM
Wu
De-Sheng
DS
D000818:Animals; D002460:Cell Line; D052244:Endocrine Disruptors / Q000633:toxicity*; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000187:drug effects* / Q000235:genetics; D013418:Sulfamethazine / Q000633:toxicity*; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D052244:Endocrine Disruptors; D011988:Receptors, Thyroid Hormone; D013418:Sulfamethazine