Fast antimicrobial screen test (FAST): improved screen test for detecting antimicrobial residues in meat tissue.
The Fast Antimicrobial Screen Test (FAST) is a simple and quick screening test developed to detect antibiotic and sulfonamide residues in food animal carcasses in slaughter establishments. This microbial inhibition test detects antimicrobials that are allowed to be used in food animals. It has the ability to detect these antimicrobials at or above the allowable limit in carcass kidney fluids in 6 h. Laboratory evaluations show that the lower limit of detection (LLD) of FAST and the Calf Antibiotic and Sulfa Test (CAST) for antibiotics tested was the same, but the LLD for sulfonamides of FAST was lower than the LLD of CAST. Compared with the Swab Test on Premises (STOP) developed in 1977, the LLD's of FAST for both antibiotics and sulfonamides were significantly better. Under field conditions, the sensitivity of FAST and CAST to antibiotic and sulfonamide residues in animal kidneys was not significantly different, but the time required by FAST was significantly lower than CAST (6 versus 18 h). Compared with the STOP, the sensitivity and the range of detection by FAST for all antimicrobials were significantly higher and the testing time was lower (18 versus 6 h).
Dey
Bhabani P
BP
U.S. Department of Agriculture, Food Safety and Inspection Service, Methods Development Laboratory, Beltsville, MD 207054, USA. Bhabani.dey@fsis.usda.gov
Thaker
Nitin H
NH
Bright
Susan A
SA
Thaler
Alice M
AM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis*; D001410:Bacillus megaterium / Q000187:drug effects; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002417:Cattle; D003470:Culture Media; D003627:Data Interpretation, Statistical; D004350:Drug Residues / Q000032:analysis*; D005188:False Negative Reactions; D005189:False Positive Reactions; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D011200:Poultry; D012015:Reference Standards; D013449:Sulfonamides / Q000032:analysis
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D003470:Culture Media; D013449:Sulfonamides