[A preliminary study of CTGF in bleomycin-induced mouse pulmonary fibrosis].
OBJECTIVE
To investigate CTGF's role in bleomycin-induced pulmonary fibrosis.


METHODS
Kunming mice were intratracheally administered with bleomycin to establish pulmonary fibrosis model. The expression of CTGF was studied by immunohistochemical method and RT-PCR. The degree of fibrosis was evaluated by pulmonary hydroxyproline assay.


RESULTS
CTGF protein and mRNA were not expressed in normal mice. However, they were detected in pulmonary fibrotic mice from day 7 after bleomycin treatment, and increased as fibrosis developed (P <0.01). CTGF protein's expression was correlated with hydroxyproline content in lung tissue (r=0.92, P <0.01).


CONCLUSIONS
CTGF plays a role in pulmonary fibrosis. Detection of CTGF may be an early and sensitive marker for evaluating the occurrence and development of pulmonary fibrosis.
Zhang
Lei
L
Department of Respiratory Disease, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China. sunray168@126.com
Lu
Hui-ping
HP
Lai
Guo-xiang
GX
D000818:Animals; D015415:Biomarkers; D001761:Bleomycin / Q000009:adverse effects*; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006909:Hydroxyproline / Q000378:metabolism; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011658:Pulmonary Fibrosis / Q000139:chemically induced* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D015415:Biomarkers; C526318:CCN2 protein, mouse; D012333:RNA, Messenger; D001761:Bleomycin; D055513:Connective Tissue Growth Factor; D006909:Hydroxyproline