Visualizing adhesion-induced agglutination of Escherichia coli with mannosylated nanoparticles.
Polymeric nanoparticles covalently functionalized with derivatized D-mannose molecules were synthesized and characterized. These nanoparticles have an average size of approximately 160 nm in diameter, thus bearing a large number of surface-tethered mannose moieties for multivalent interactions with adhesins on bacterial cells. Specifically, the mannosylated nanoparticles bind strongly with Escherichia coli, allowing the convenient visualization of adhesion interactions under a conventional electron microscope. Since a single nanoparticle is capable of binding more than one cell, the adhesion interactions result in significant nanoparticle-mediated cell agglutination according to electron microscopy imaging. Potential applications of the mannosylated nanoparticles in the inhibition of enteropathogenic infections are discussed.
Qu
Liangwei
L
Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.
Luo
Pengju G
PG
Taylor
Shelby
S
Lin
Yi
Y
Huang
Weijie
W
Anyadike
Nkechinyere
N
Tzeng
Tzuen-Rong J
TR
Stutzenberger
Fred
F
Latour
Robert A
RA
Sun
Ya-Ping
YP
D018830:Adhesins, Escherichia coli / Q000378:metabolism; D000371:Agglutination / Q000187:drug effects / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008358:Mannose / Q000737:chemistry* / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods
D018830:Adhesins, Escherichia coli; D008358:Mannose