Protective effect of antiganglioside antibodies against experimental Trypanosoma brucei infection in mice.
Liposome-associated ganglioside antigens (ganglioside GM1 or bovine brain gangliosides) were prepared to facilitate the potential protective efficacy for Trypanosoma brucei. Mice were immunized with liposome-associated ganglioside GM1 or bovine brain gangliosides intraperitoneally (i.p.). After immunization, significantly higher antigen-specific IgG and IgM antibodies were detected in sera than in the nonimmunized control group. When sera from immunized mice were analyzed for isotype distribution, antigen-specific IgG1, IgG2a, and IgG3 antibody responses were also noted. After immunization, mice were challenged i.p. with 1 x 10(2) cells of T. brucei. Sixty percentage of liposome-associated ganglioside GM1-immunized mice survived the infection, and all the mice immunized with bovine brain gangliosides-containing liposomes survived. However, all control mice died within 7 days after infection. These data demonstrate that liposomes containing ganglioside antigens have the potential usefulness for the induction of a protective immune response against T. brucei infection and suggest the possibility of developing vaccines that may ultimately be used for the prevention of trypanosomiasis.
Uemura
Akiko
A
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
Watarai
Shinobu
S
Ohnishi
Yoshihiro
Y
Kodama
Hiroshi
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007274:Injections, Intraperitoneal; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000517:prevention & control*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D005732:Gangliosides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008081:Liposomes; D005677:G(M1) Ganglioside