title: [Research on gene transfer by spermatozoa].
abstract: Incubate golden hamster spermatozoa and NF (kappa) B-luc(+) in co-culture and liposome mediated methods, and then process the IVF with oocytes, And two groups of samples were detected by PCR, Southern Blot and FISH. Analyzed the relative data by statistics. The results showed that spermatozoa of Golden hamster epididymis can take the foreign DNA voluntarily, and the foreign DNA exist in the head of the spermatozoa can enter into zygotes by IVF. The exist of liposome can improve the foreign gene transfect ratio and transgenic ratio.
authors.affiliation: School of Public Health, Lanzhou University, Lanzhou 730000, China. xmwang@szu.edu.cn
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Ye
authors.initials: Y
authors.lastname: Ruan
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Xie
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Huang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D018920:Coculture Techniques; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017404:In Situ Hybridization, Fluorescence; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013077:Sperm Head / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D015053:Zygote / Q000166:cytology / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D008156:Luciferases