Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture.
Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1 and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d 1 was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation.
Dohi
Yoshiko
Y
Department of Public Health, Nara Medical University, Kashihara City, Japan.
Shimaoka
Hideki
H
Ikeuchi
Masako
M
Ohgushi
Hajime
H
Yonemasu
Kunio
K
Minami
Takeshi
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001846:Bone Development / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D015151:Immunoblotting; D007536:Isomerism; D008297:Male; D008648:Mesoderm / Q000166:cytology; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000893:Anti-Inflammatory Agents; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012313:RNA; D003907:Dexamethasone; D008668:Metallothionein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000469:Alkaline Phosphatase; D002118:Calcium