title: The role of bone marrow-derived cells in bone fracture repair in a green fluorescent protein chimeric mouse model.
abstract: We investigated the role of bone marrow cells in bone fracture repair using green fluorescent protein (GFP) chimeric model mice. First, the chimeric model mice were created: bone marrow cells from GFP-transgenic C57BL/6 mice were injected into the tail veins of recipient wild-type C57BL/6 mice that had been irradiated with a lethal dose of 10Gy from a cesium source. Next, bone fracture models were created from these mice: closed transverse fractures of the left femur were produced using a specially designed device. One, three, and five weeks later, fracture lesions were extirpated for histological and immunohistochemical analyses. In the specimens collected 3 and 5 weeks after operation, we confirmed calluses showing intramembranous ossification peripheral to the fracture site. The calluses consisted of GFP- and osteocalcin-positive cells at the same site, although the femur consisted of only osteocalcin-positive cells. We suggest that bone marrow cells migrated outside of the bone marrow and differentiated into osteoblasts to make up the calluses.
authors.affiliation: Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan. s3061@nms.ac.jp <s3061@nms.ac.jp>
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Taguchi
authors.forename: Rei
authors.initials: R
authors.lastname: Ogawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Migita
authors.forename: Hideki
authors.initials: H
authors.lastname: Hanawa
authors.forename: Hiromoto
authors.initials: H
authors.lastname: Ito
authors.forename: Hideo
authors.initials: H
authors.lastname: Orimo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002146:Bony Callus / Q000737:chemistry / Q000000981:diagnostic imaging / Q000473:pathology; D002678:Chimera; D004195:Disease Models, Animal; D017102:Fracture Healing; D050723:Fractures, Bone / Q000000981:diagnostic imaging / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011859:Radiography; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D049452:Green Fluorescent Proteins