Glycyrrhizin inhibits R5 HIV replication in peripheral blood monocytes treated with 1-methyladenosine.
R5 HIV replicated in freshly isolated human peripheral blood monocytes after treatment with 1-methyladenosine (fresh PBM/MA), an immunosuppressive compound isolated from tumorous ascites fluids, while viral replication was not demonstrated in untreated peripheral blood monocytes (fresh PBM). The R5 HIV replication in fresh PBM/MA was inhibited by glycyrrhizin (GL). Without any other stimulation, fresh PBM/MA produced CCL2 and IL-10, while these soluble factors were not released from fresh PBM. GL greatly inhibited the production of CCL2 and IL-10 in fresh PBM/MA. After treatment with CCL2 and/or IL-10, CCR5 mRNA expression in fresh PBM was markedly enhanced, while only a trace level of the mRNA expression was detected in these cells in the absence of CCL2 or IL-10. CCR5 mRNA expression in fresh PBM treated with CCL2 or IL-10 was clearly inhibited by GL. These results indicate that GL inhibits R5 HIV replication in fresh PBM/MA through the inhibiting CCR5 expression mediated by CCL2 or IL-10.
Takei
Miwa
M
Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555-0435, USA.
Kobayashi
Makiko
M
Li
Xiao-Dong
XD
Pollard
Richard B
RB
Suzuki
Fujio
F
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019713:Receptors, CCR5 / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
D000890:Anti-Infective Agents; D018932:Chemokine CCL2; D012333:RNA, Messenger; D019713:Receptors, CCR5; D016753:Interleukin-10; C002230:1-methyladenosine; D019695:Glycyrrhizic Acid; D000241:Adenosine