[Preparation and characterization of monoclonal antibodies against human RANTES molecule].
OBJECTIVE
To prepare monoclonal antibodies against human RANTES molecule and identify the expression of RANTES in rat small intestine after small bowel transplantation.


METHODS
Murine mAbs were prepared by B lymphocyte hybridoma technique. The expression of RANTES in rat small intestine after small bowel transplantation was detected by immunohistochemistry.


RESULTS
Four hybridoma cell lines secreting monoclonal antibodies to human RANTES, FMU-RANTES 1, FMU-RANTES 2, FMU-RANTES 3 and FMU-RANTES 4, were established. The titers of a scetic mAbs reached to 1 x 10(-6) and the Ig subclass of FMU-RANTES 1, FMU-RANTES 3 and FMU-RANTES 4 was IgG1(kappa) and that of FMU-RANTES 2 was IgG2b(kappa). Among these mAbs, FMU-RANTES 1, FMU-RANTES 2 and FMU-RANTES 3 could bind human RANTES protein in Western bolt. FMU-RANTES 1, FMU-RANTES 2 and FMU-RANTES 4 could be used in immunohistochemistry staining. Rat RANTES molecule could be detected in the cyto plasm of epithelial cells in rat small intestine after small bowel transplantation.


CONCLUSIONS
Four mAbs against RANTES molecule were prepared, which can provide a useful tool in research on the structure and function of RANTES molecule. High expression of RANTES may be involved in the rejection of allogeneic graft.
Kang
Zhen-hua
ZH
Department of Gastrointestinal Surgery, Fourth Military Medical University, Xi'an 710032, China. weichang@fmmu.edu.cn
Wang
Wei-zhong
WZ
Chen
Dong-li
DL
Wang
Chun-yan
CY
Zhu
Can-sheng
CS
Du
Jun-feng
JF
Jin
Bo-quan
BQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000276:immunology* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007422:Intestines / Q000166:cytology; D051381:Rats
D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5