Pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium Shewanella violacea DSS12.
The genes of cytochrome bd-encoding cydAB were identified from a deep-sea bacterium Shewanella violacea DSS12. These showed significant homologies with known cydAB gene sequences from various organisms. Additionally, highly conserved regions that are important for the enzymatic function were also conserved in cydA of S. violacea. Based on the results, transcriptional analysis of cydAB operon and cydDC operon (required for assembly of cytochrome bd) of S. violacea in microaerobic condition was performed under the growth condition of various pressures. The gene of cydA was expressed even under the condition of atmospheric pressure and its expression was enhanced with pressurization. On the other hand, the expression of cydC was strongly depressed under the condition of atmospheric pressure compared with the case under high pressure. It appeared spectrophotometrically that loss of cytochrome bd in S. violacea under atmospheric pressure shown in previous study is caused mainly by the loss of cydDC. Further, under the growth condition of atmospheric pressure, either less amount or no d-type cytochrome was expressed compared with the case of high-pressure condition even if the organism was grown under alkaline condition or in the presence of uncoupler, which are the inducible condition of d-type cytochrome in Escherichia coli. These results suggested that the significant amount of d-type cytochrome expression is specific event under the growth condition of high pressure.
Tamegai
Hideyuki
H
Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan. htamegai@chs.nihon-u.ac.jp
Kawano
Hiroaki
H
Ishii
Akihiro
A
Chikuma
Sayaka
S
Nakasone
Kaoru
K
Kato
Chiaki
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D017937:Cytochrome d Group / Q000096:biosynthesis / Q000235:genetics; D003580:Cytochromes / Q000096:biosynthesis* / Q000235:genetics; D045303:Cytochromes b / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers; D008969:Molecular Sequence Data; D009876:Operon; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011312:Pressure; D012623:Seawater / Q000382:microbiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020592:Shewanella / Q000201:enzymology*
D003580:Cytochromes; D017931:DNA Primers; D017937:Cytochrome d Group; D045303:Cytochromes b