Involvement of Saccharomyces cerevisiae Pdr5p ATP-binding cassette transporter in calcium homeostasis.
Deletion of PDR5 gene (Deltapdr5) in Saccharomyces cerevisiae led to increased resistance to calcium. The cellular Ca2+ level in the presence of high calcium as estimated by reporter assay in Deltapdr5 cells was significantly lower than that in wild-type cells. Membrane Pdr5p levels diminished rapidly during incubation with high calcium in a manner dependent on calcineurin and Pep4p, suggesting a feedback regulatory mechanism for Pdr5p abundance.
Tutulan-Cunita
Andreea Cristina
AC
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Ohnishi
Tomoko
T
Mizunuma
Masaki
M
Hirata
Dai
D
Miyakawa
Tokichi
T
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006706:Homeostasis; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; C092438:PDR5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002118:Calcium