Axenic cultivation of Entamoeba dispar in newly designed yeast extract-iron-gluconic acid-dihydroxyacetone-serum medium.
Yeast extract-iron-gluconic acid-dihydroxyacetone-serum medium that allows axenic cultivation of Entamoeba dispar was designed based on casein-free yeast extract-iron-serum (YI-S) medium, and the usefulness of the medium was assessed. The main differences from YI-S medium are replacement of glucose by gluconic acid, addition of dihydroxyacetone and D-galacturonic acid monohydrate, and sterilization by filtration. This medium promoted the axenic growth of 5 strains of E. dispar (2 strains of nonhuman primate isolates and 3 strains of human isolates). In addition, to clarify the biological basis for the growth of E. dispar in this medium, analyses of relevant enzymes on the glycolytic pathway of the amoebae as well as of the protozoans that are the best culture supplement for amoebae are being performed.
Kobayashi
Seiki
S
Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. skobaya@sc.itc.keio.ac.jp
Imai
Eiko
E
Haghighi
Ali
A
Khalifa
Shaden A
SA
Tachibana
Hiroshi
H
Takeuchi
Tsutomu
T
D000818:Animals; D016831:Crithidia fasciculata / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D004098:Dihydroxyacetone / Q000378:metabolism; D004747:Entamoeba / Q000254:growth & development*; D005942:Gluconates / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D044967:Serum / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry
D003470:Culture Media; D005942:Gluconates; D007501:Iron; D004098:Dihydroxyacetone; C030691:gluconic acid