High-throughput assays for lipases and esterases.
In the past few years a considerable number of high-throughput screening (HTS) systems have been developed, especially for lipases and esterases. In this review, a range of HTS methods for the directed evolution of these hydrolases are covered. This includes spectrophotometric and fluorimetric formats as well as other approaches to allow for fast, efficient and reliable identification of desired enzyme variants within large mutant libraries. In addition, methods for library creation and application of lipases and esterases are briefly covered.
Schmidt
Marlen
M
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr. 16, D-17487 Greifswald, Germany.
Bornscheuer
Uwe T
UT
D019020:Directed Molecular Evolution / Q000379:methods; D004950:Esterases / Q000032:analysis* / Q000235:genetics; D015723:Gene Library; D008049:Lipase / Q000032:analysis* / Q000235:genetics
D004950:Esterases; D008049:Lipase