Influence of dietary genistein levels on tissue genistein deposition and on the physical, chemical, and sensory quality of rainbow trout, Oncorhynchus mykiss.
Genistein, the primary isoflavone in soybean, is one of the chemical components responsible for some of the off-flavors associated with soy-based foods. The potential effects of genistein on the sensory and chemical quality of fish muscle may affect the full utilization of soybean meal as an alternative protein in aquaculture diets. Fingerling trout fed commercial diets containing 0, 500, 1000, or 3000 ppm pure genistein were analyzed after 6 and 12 months of feeding. Genistein was extracted by enzymatic digestions in Tris buffer and quantified by high-performance liquid chromatography. Moisture, fat, protein, ash, and tristimulus color of the fillets were determined. The extent of lipid oxidation occurring in fillets harvested after 12 months of feeding was studied by measurements of thiobarbituric acid reactive substances (TBARS) after 4 and 8 days of refrigerated storage at 4 degrees C. Triangle tests were performed to determine if there were any detectable sensory differences. A dietary genistein content of 3000 ppm led to the deposition of approximately 5.4 pmol of genistein/mg of fillet. Triangle test panelists were unable to detect any significant (p < or = 0.05) differences between the fillets from trout fed the 0 and 3000 ppm genistein concentrations. Moisture, ash, and protein content were influenced by time of harvest, while color was unaffected. TBARS levels on days 4 and 8 were significantly (p < 0.05) higher in the fillets from the 0 ppm genistein level than in fillets from fish fed dietary genistein.
D'Souza
Natasha
N
Department of Food Science and Human Nutrition, University of Maine, Orono, Maine 04469, USA.
Skonberg
Denise I
DI
Camire
Mary E
ME
Guthrie
Kelly E
KE
Malison
Jeffrey
J
Lima
Lucienne
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004032:Diet; D019833:Genistein / Q000008:administration & dosage* / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D015227:Lipid Peroxidation; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D013025:Glycine max; D013649:Taste; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D019833:Genistein