[Legionella pneumophila: extraction of the main protein from the outer membrane (p29)].
Legionella pneumophila is an important opportunistic pulmonary pathogen that causes Legionaires'diseases. In the outer membrane of the bacterium, there is a protein with molecular weight of 29 kD(p 29), which is not only an specific antigen of the species but also the major outer membrane protein; it may also be a factor of pathogenecity in the bacterium. The main objective of this paper was the extraction and purification of this protein by treating the outer membrane with chemical agents. A p 29-rich semipurified antigen extract was obtained that can be used as an important antigen for the development of immunodiagnostic techniques in future studies and also for obtaining a monoclonal antibody to be used for the same purpose.
Sosa
Nibaldo L González
NL
Laboratorio de Investigaciones del SIDA, Ciudad de La Habana, Cuba. cicdc@infomed.sld.cu
Alfonso
Lázaro Regalado
LR
González
Martha J Alfonso
MJ
de Armas
Madeline Blanco
MB
Sui
Otto Cruz
OC
D000947:Antigens, Helminth / Q000302:isolation & purification*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D016952:Legionella pneumophila / Q000737:chemistry*
D000947:Antigens, Helminth; D001425:Bacterial Outer Membrane Proteins; C406723:P-29 antigen