Screening candidate genes for mutations in patients with hypogonadotropic hypogonadism using custom genome resequencing microarrays.
OBJECTIVE
The purpose of this study was to determine the consistency of calling single nucleotide polymorphisms (SNPs) by custom genome resequencing microarrays compared with capillary DNA sequencing.


METHODS
Amplified genomic DNA from 23 patients with hypogonadotropic hypogonadism was hybridized to microarrays containing 30 kilobases of sequence from 6 different candidate genes. Capillary DNA sequencing was performed in 10 patients.


RESULTS
For 10 patients with > or =90% of bases called, 49 SNPs in 5 of 6 genes were identified. Of the 490 bases, 75 were ambiguous (read as "N"), and 415 were able to be called an A, C, G, or T. Of 415 called, 401 (96.6%) sequences were confirmed by DNA sequencing. All homozygotes (285/285) were called identically, while sequence from 89.2% (116/130) of heterozygotes agreed by both methods. The level of agreement between microarray calls and capillary DNA sequencing demonstrated substantial accuracy.


CONCLUSIONS
Custom genome resequencing microarrays are highly consistent with capillary sequencing in calling individual bases in genomic DNA from patients with human disease.
Xu
Ning
N
Section of Reproductive Endocrinology, Infertility, and Genetics, Department of Obstetrics and Gynecology, The Medical College of Georgia, Augusta, GA 30912, USA.
Podolsky
Robert H
RH
Chudgar
Pranav
P
Chorich
Lynn P
LP
Liu
Chunmei
C
McDonough
Paul G
PG
Warrington
Janet A
JA
Layman
Lawrence C
LC
D000293:Adolescent; D001483:Base Sequence; D015331:Cohort Studies; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D015894:Genome, Human; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D006065:Gonadotropins, Pituitary / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007006:Hypogonadism / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D050809:POU Domain Factors; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics
D037201:Follicle Stimulating Hormone, beta Subunit; D006065:Gonadotropins, Pituitary; C489736:HESX1 protein, human; D018398:Homeodomain Proteins; C411985:NSMF protein, human; D050809:POU Domain Factors; D014157:Transcription Factors; C085076:transcription factor Brn-2; D007987:Gonadotropin-Releasing Hormone