title: Cutaneous tuberculosis diagnosis in an inhospitable Amazonian region by means of telemedicine and molecular biology.
abstract: We report on a 13-year-old boy who displayed a chronic granulomatous inflammatory reaction of 5 years duration. The lesion was resistant to different antibiotic schemes; his routine laboratory tests and chest radiographs were normal. Teledermatologic consultation and histopathologic study of skin biopsy suggested scrofulodermal tuberculosis. Polymerase chain reaction amplification of DNA extracted from lymph node biopsy was taken as starting material for dot-blot hybridization using Mtp-40 and IS 6110 as probes for detecting either Mycobacterium tuberculosis or any mycobacteria belonging to the M tuberculosis complex, respectively. Positive results in both hybridizations were further confirmed by culturing in BACTEC MGIT 960 system. The lesion greatly diminished following isoniazid, rifampin, and ethambutol treatment. Telemedicine allowed a cutaneous tuberculosis diagnosis to be made of a patient living in a remote town located in the Amazon jungle by using molecular biology techniques.
authors.affiliation: Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogotá, Colombia.
authors.forename: Diana I
authors.initials: DI
authors.lastname: Angel
authors.forename: Rosalba
authors.initials: R
authors.lastname: Alfonso
authors.forename: Michel
authors.initials: M
authors.lastname: Faizal
authors.forename: Orlando
authors.initials: O
authors.lastname: Ricaurte
authors.forename: Julio A
authors.initials: JA
authors.lastname: Baez
authors.forename: Andres
authors.initials: A
authors.lastname: Rojas
authors.forename: Paola
authors.initials: P
authors.lastname: Barato
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000293:Adolescent; D001706:Biopsy; D003105:Colombia; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012424:Rural Population; D017216:Telemedicine; D014382:Tuberculosis, Cutaneous / Q000473:pathology*
chemical_list: D004269:DNA, Bacterial