Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation.
The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties.
Bellik
Lydia
L
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Ledda
Fabrizio
F
Parenti
Astrid
A
D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D004705:Endocytosis; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007797:Laminin / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000502:physiology; D008027:Light; D010641:Phenotype; D011509:Proteoglycans / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012542:Scattering, Radiation; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D013997:Time Factors
D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen