Improvement of a multiplex PCR system for DYS441, DYS442, DYS443, DYS444 and DYS445, and a population study in 340 Japanese males.
A multiplex PCR system for five Y-STRs (DYS441, DYS442, DYS443, DYS444 and DYS445) has been improved to increase the probability of obtaining a DNA typing result from aged samples. Newly designed PCR primers for amplification of the DYS441 and DYS442 loci and optimization of PCR conditions enabled successful typing from blood and semen stains that had been stored for more than seven years at room temperature. Analysis of 340 Japanese males revealed 7, 5, 6, 5 and 4 alleles at the DYS441, DYS442, DYS443, DYS444 and DYS445 loci, respectively, yielding 122 haplotypes with a cumulative haplotype diversity of 0.97.
Ogata
Yasuhiro
Y
Research Institute of Scientific Criminal Investigation, Fukui Prefectural Police Headquarters, Fukui 910-0005, Japan.
Yamaba
Tomokazu
T
Sawazaki
Kazumi
K
Iida
Reiko
R
Tsubota
Etsuko
E
Takatsuka
Hisakazu
H
Matsuki
Takasumi
T
Yasuda
Toshihiro
T
Kishi
Koichiro
K
D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000737:chemistry; D013048:Specimen Handling; D020080:Tandem Repeat Sequences; D013997:Time Factors
D017931:DNA Primers; D004247:DNA