Expression of CD158b on peripheral blood lymphocytic cell after kidney transplantation.
OBJECTIVE
To investigate the expression of CD158b on peripheral blood lymphocytes after kidney transplantation.


METHODS
Sixty two kidney transplant patients were divided into two groups (normal group and rejection group) according to pathologic results and clinical situation. Blood samples were assessed for percentage of CD3+; CD19+; CD3-CD16/56+; CD3+CD158b+; CD19+CD158b+, and CD3-CD16/56+CD158b+ subsets.


RESULTS
The percentages of CD3+ cells preop as well as at 1 and 7 postoperative and the day acute rejection happened were 60.06 +/- 4.67, 40.43 +/- 4.11, 31.67 +/- 4.04, and 39.21 +/- 5.20, respectively. The percentages of CD3-CD16/56+ were 21.65 +/- 1.79, 33.84 +/- 5.45, 38.10 +/- 4.86, and 39.53 +/- 4.80, respectively. The percentages of CD3+CD158b+ were 1.46 +/- 0.31, 1.88 +/- 0.70, 2.03 +/- 1.04, and 0.65 +/- 0.12, respectively. The percentages of CD3-CD16/56+CD158b+ were 5.87 +/- 1.24, 3.57 +/- 0.57, 2.82 +/- 0.45, and 1.60 +/- 0.33, respectively.


CONCLUSIONS
The percentage of CD3+ cells in the normal and the rejection groups decreased significantly. The percentages of CD158b+T cells decreased significantly after acute rejection. The percentage of CD158b+NK cells decreased significantly after kidney transplantation, decreasing gradually after acute rejection. The percentage of CD158b+ total T cells decreased significantly following acute rejection. The percentage of CD3-CD16/56+CD158b+ of total NK cells decreased significantly after kidney transplantation and after acute rejection. Because few factors interfere with the expression of CD158b on NK cells, monitoring of this marker may be accurate and sensitive.
Kang
N
N
The Department of Urology and Pathology, Beijing Chaoyang Hospital, Beijing, China.
Guan
D
D
Xing
N
N
Xia
C
C
D015703:Antigens, CD / Q000097:blood*; D001402:B-Lymphocytes / Q000382:microbiology; D017252:CD3 Complex / Q000097:blood; D005786:Gene Expression Regulation / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D011184:Postoperative Period; D011971:Receptors, Immunologic / Q000097:blood*; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D017252:CD3 Complex; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3