Species identification by means of pyrosequencing the mitochondrial 12S rRNA gene.
The aim of this study was to develop a new method for species identification based on the analysis of a very short nucleotide sequence. For this reason, the mitochondrial 12S rRNA gene, together with the new method of pyrosequencing, was used. The detection of only 20 nucleotides, following the sequencing primer within a 149-bp fragment by pyrosequencing, was sufficient to identify the biological origin of the samples by alignment with a reference sequence database. A case example with a piece of skin is presented, and the question whether this piece of skin came from a missing wife or from an animal could be answered.
Balitzki-Korte
B
B
Department of Legal Medicine, University Hospital Giessen, Frankfurter Strasse 58, 35392 Giessen, Germany. Beate.Balitzki-Korte@gmx.net
Anslinger
K
K
Bartsch
C
C
Rolf
B
B
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D001483:Base Sequence; D005554:Forensic Medicine; D006801:Humans; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary*; D013045:Species Specificity
D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012313:RNA