title: [Influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice].
abstract: OBJECTIVE
  To investigate the influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice.
  
  
  METHODS
  C57BL/6J mice, exposed to hypoxia in the man-made auto pressure and hypoxia control cabin (XQ-I), were divided into 3 groups: the normal control group, 24 h hypoxia group and 48 h hypoxia group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of leptin and leptin receptor.
  
  
  RESULTS
  (1) Compared with the normal control group (0.508 +/- 0.207), the leptin mRNA level of 24 h hypoxia group (0.903 +/- 0.190) and 48 h hypoxia group (0.856 +/- 0.336) were up-regulated markedly (all < 0.05); (2) Compared with the normal control group (Ra 0.630, Rb 0.258 +/- 0.049, Rc 0.133), the Ra, Rb, Rc mRNA levels of 24 h hypoxia group (Ra 0.724, Rb 0.381 +/- 0.038, Rc 0.299) and 48 h hypoxia group (Ra 0.700, Rb 0.345 +/- 0.042, Rc 0.292) were up-regulated to different degrees.
  
  
  CONCLUSIONS
  As an independent factor, hypoxia can stimulate increased expression of leptin, leptin receptor Ra, Rb, Rc mRNA. It is suggested that leptin play an important role in positive regulation of respiratory function.
authors.affiliation: Department of Gerontology, Third People's Hospital, Chongqing 400014, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Ya-mei
authors.initials: YM
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Tang
authors.forename: Yin-bao
authors.initials: YB
authors.lastname: Zhong
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000860:Hypoxia; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D054411:Receptors, Leptin; D013997:Time Factors
chemical_list: D020738:Leptin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse