[Study on the effect of aluminum (III) on NADH in the presence of NH4Ac in aqueous solution].
This paper studied the interactions of Al (III ) and dihydronicotinamide adenine dinucleotide (NADH) in nearly neutral aqueous solutions (pH 6.5) by means of UV-Vis and 1H, 13C-NMR techniques. The results suggested that Al (III) interacts with NADH to form Al-NADH complexes by occupying the binding sites of phosphate oxygen atoms O(N)1 and O(A)1 and ribose ring hydroxyl groups, which are the potential recognition sites for substrates, coenzyme and enzyme. In the presence of NH4Ac salt buffer and with Al (III) salt solution, NADH will be marked with structural changes at the nicotinamide moiety in contrast with almost no structural changes in Tris-HCl buffer solution with Al (III) salt.
Yang
Xiao-Di
XD
State Key Laboratory of Coordination Chemistry, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Miao
Qiang
Q
Qian
Sheng-Li
SL
Bi
Shu-Ping
SP
D000085:Acetates / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
D000085:Acetates; D012996:Solutions; D014867:Water; D009243:NAD; D000535:Aluminum; C018824:ammonium acetate