title: Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support.
abstract: BACKGROUND
  Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
  
  
  RESULTS
  Using surface-bound peptide nucleic acids (PNA) probes and soluble fluorescent cationic polythiophenes, we show a simple and sensitive electrostatic approach to detect and identify unlabelled target nucleic acid on microarray.
  
  
  CONCLUSIONS
  This simple methodology opens exciting possibilities for applied genetic analysis for the diagnosis of infections, identification of genetic mutations, and forensic inquiries. This electrostatic strategy could also be used with other nucleic acid detection methods such as electrochemistry, silver staining, metallization, quantum dots, or electrochemical dyes.
authors.affiliation: Centre de Recherche en Infectiologie, l'Université Laval, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Sainte-Foy, Québec, G1V 4G2, Canada. fraymond@scientist.com
authors.forename: Frédéric R
authors.initials: FR
authors.lastname: Raymond
authors.forename: Hoang-Anh
authors.initials: HA
authors.lastname: Ho
authors.forename: Régis
authors.initials: R
authors.lastname: Peytavi
authors.forename: Luc
authors.initials: L
authors.lastname: Bissonnette
authors.forename: Maurice
authors.initials: M
authors.lastname: Boissinot
authors.forename: François J
authors.initials: FJ
authors.lastname: Picard
authors.forename: Mario
authors.initials: M
authors.lastname: Leclerc
authors.forename: Michel G
authors.initials: MG
authors.lastname: Bergeron
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D002412:Cations; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology*; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000494:pharmacology*; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D055672:Static Electricity; D013876:Thiophenes / Q000737:chemistry
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D009696:Nucleic Acids; D020135:Peptide Nucleic Acids; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D004247:DNA