[Effects of diethylhexyl phthalate on lipid peroxidation and the life-span in Drosophila melanogaster].
OBJECTIVE
To observe the effects of diethlhexyl phthalate (DEHP) on lipid peroxidation and the life span in Drosophila melanogaster.


METHODS
Fed Drosophila with the concentration 0.20% DEHP of exposure after 0, 14, 28 days, the activity of total superoxide dismutase (SOD), CuZn-SOD and the concentration of malondialdehyde were determined. At the same time, the longevity test was carried out to examine the effect of DEHP on the Drosophila's lifespan.


RESULTS
The lifespan of Drosophila was shortened in a dose of DEHP exposed groups. The indexes of mean life span (MLS), 50% lethal time and mean maximum life span in three DEHP-treated groups (concentration of 0.05%, 0.10% and 0.20%) were lower than those of the controlled group respectively (P < 0.01 or P < 0.05). The MLS of both Drosophila sexes were reduced from the control of 64 days and 59 days to the test 60 days-52 days and 54 days-49 days respectively. DEHP decreased the activity of SOD (P < 0.01 or P < 0.05), and lead to a time-dependent relation and an increase in the concentration of malondialdehyde (P < 0.01 or P < 0.05) in the DEHP-exposed Drosophila groups.


CONCLUSIONS
DEHP might promote the process of lipid peroxidation and shorten the life span in Drosophila melanogaster. It should be one of the reasons in the senescence of Drosophila.
Li
Shu-guang
SG
Department of Preventive Medicine, Medical College, Tongji University, Shanghai 200092, China.
Huang
Xin
X
Zhang
Xin-wen
XW
Xu
Si-hong
SH
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D008136:Longevity / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D008315:Malondialdehyde; D004051:Diethylhexyl Phthalate; D013482:Superoxide Dismutase