title: The effect of growth regulators and sucrose on anthocyanin production in Camptotheca acuminata cell cultures.
abstract: The effect of different concentrations of growth regulators and sucrose on anthocyanin production in cell suspension cultures of Camptotheca acuminata Decaisne (Nyssaceae) was described for the first time and qualitatively and quantitatively evaluated. Anthocyanin production was significantly greater in the presence of kinetin, compared to benzyladenine, with the greatest concentration observed in the presence of 2 microM kinetin. No significant differences in anthocyanin production were observed when comparing 2,4-dichlorophenoxyacetic acid to alpha-naphthaleneacetic acid, except when using 2 microM, 2,4-dichlorophenoxyacetic acid, which resulted in greater anthocyanin production. High sucrose concentration enhanced the production of anthocyanins. Based on the absence of anthocyanin production in the dark, we concluded that light was essential for stimulating anthocyanin production. The optimised medium consisted of: 2 microM kinetin, 2 microM 2,4-dichlorophenoxyacetic acid and 292 mM sucrose. HPLC/DAD and HPLC/MS analyses revealed that the main anthocyanin was Cy 3-O-galactoside and that the minor derivative was Cy 3-O-glucoside.
authors.affiliation: Dipartimento di Biologia Vegetale, Università degli Studi di Roma "La Sapienza", Piazzale Aldo Moro 5, 00185 Rome, Italy. gabriella.pasqua@uniroma1.it
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pasqua
authors.forename: Barbara
authors.initials: B
authors.lastname: Monacelli
authors.forename: Nadia
authors.initials: N
authors.lastname: Mulinacci
authors.forename: Simona
authors.initials: S
authors.lastname: Rinaldi
authors.forename: Catia
authors.initials: C
authors.lastname: Giaccherini
authors.forename: Marzia
authors.initials: M
authors.lastname: Innocenti
authors.forename: Franco Francesco
authors.initials: FF
authors.lastname: Vinceri
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D029769:Camptotheca; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D010937:Plant Growth Regulators / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology*; D013549:Sweetening Agents / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D010937:Plant Growth Regulators; D013549:Sweetening Agents; D013395:Sucrose