Tumor immunity and prolonged survival following combined adenovirus-HSP72 and CEA-plasmid vaccination.
We have studied the effects of recombinant adenoviruses as immune adjuvants for DNA vaccination. In a mouse model, using the weak immunogen carcinoembryonic antigen (CEA), anti-CEA IgG production was significantly higher and occurred earlier when immunization included a recombinant adenovirus together with CEA-plasmid DNA. Combined immunization with a recombinant adenovirus expressing the immunomodulatory molecule heat shock protein 72 (ADHSP72) and CEA-plasmid DNA resulted in CEA-specific T-cell activation capable of protecting mice from tumor formation with CEA expressing cells. Additionally, animals with CEA expressing tumors showed diminished tumor growth and prolonged survival when immunized with ADHSP72 and CEA-plasmid DNA compared to controls. Recombinant adenoviruses expressing immunomodulatory molecules such as HSP72 may be useful adjuvants for DNA vaccination.
Myers
Adrienne L
AL
School of Medicine, Saint Louis University, St. Louis, MO 63110, USA.
Krewet
James A
JA
Shah
Maulik R
MR
D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007156:Immunologic Memory / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control; D016019:Survival Analysis; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic