Generation and characterization of monoclonal antibodies to human PCTAIRE 3.
Monoclonal antibodies specific for the unique N-terminal domain of the PCTAIRE 3 protein kinase have been produced and characterized. The specificity of these antibodies has been assessed by ELISA, Western blot, and immunoflorescence techniques. These reagents are specific for recombinant PCTAIRE 3 of human origin and do not cross-react with PCTAIRE 1 or PCTAIRE 2, despite the high homology between members of the PCTAIRE subfamily of cdc2-like kinases. Immunoblotting of transfected cells reveals that all of the antibodies recognize both isoforms of the PCTAIRE 3 kinase. Epitope mapping reveals three distinct classes of PCTAIRE 3 antibodies that recognize different sites within an immunodominant region of the N-terminal domain of the protein. The creation of highly specific PCTAIRE 3 monoclonal reagents will be useful for assessing the native distribution and function of this protein.
Herskovits
A Z
AZ
Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA. aherskov@aecom.yu.edu
Davies
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D019556:COS Cells; D002522:Chlorocebus aethiops; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D018844:Cyclin-Dependent Kinases; C089464:PCTAIRE-3 protein kinase