Annexins I and IV inhibit Staphylococcus aureus attachment to human macrophages.
Annexins are a family of proteins that bind to phospholipids and carbohydrates in a calcium-dependent manner. They are present in a variety of body fluids. Previous studies have shown that annexins have anti-inflammatory activities for lipid A of Gram-negative bacteria. The present study investigated the effect of annexins on interaction between Gram-positive bacteria and immune cells such as macrophages. Annexins I and IV bound to lipoteichoic acids which are surface molecules on Gram-positive bacteria. Binding of annexins I and IV to whole Staphylococcus aureus (S. aureus) were observed and these bindings were inhibited by lipoteichoic acid from S. aureus. Moreover, annexins I and IV suppressed the attachment of S. aureus to phorbol 12-myristate 13-acetate-treated THP-1 cells (human macrophages). These results suggest that annexins I and IV have ligand specificities toward foreign substances, and that the annexins might have some anti-inflammatory property for Gram-positive bacteria.
Gotoh
Mari
M
Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610, Japan.
Takamoto
Yukiko
Y
Kurosaka
Kahori
K
Masuda
Junko
J
Ida
Michiru
M
Satoh
Ayano
A
Takayama
Eiji
E
Kojima-Aikawa
Kyoko
K
Kobayashi
Yoshiro
Y
Matsumoto
Isamu
I
D017305:Annexin A1 / Q000378:metabolism / Q000494:pharmacology*; D017314:Annexin A4 / Q000378:metabolism / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000382:microbiology*; D010703:Phorbol Esters / Q000494:pharmacology; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology*; D013682:Teichoic Acids / Q000378:metabolism
D017305:Annexin A1; D017314:Annexin A4; D008070:Lipopolysaccharides; D010703:Phorbol Esters; D013682:Teichoic Acids; C009900:lipoteichoic acid; C011986:phorbol 12-tiglate 13-decanoate; D016650:Fluorescein-5-isothiocyanate