Overexpression of the mineralocorticoid receptor protects against injury in PC12 cells.
Mineralocorticoid receptor expression is increased following neuronal injury, and this is associated with increased neuronal survival. Here we demonstrate a causal link between overexpression of MR in PC12 cells and protection against death induced by staurosporine and oxygen-glucose deprivation. This survival effect is abrogated by MR antagonism. Drugs which upregulate MR may form the basis for a novel therapeutic approach in conditions such as stroke and head injury.
Lai
Maggie
M
Endocrinology Unit, Molecular Medicine Centre, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, Scotland, UK. m.lai@ed.ac.uk
Seckl
Jonathan
J
Macleod
Malcolm
M
D000450:Aldosterone / Q000493:pharmacokinetics; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000633:toxicity; D005786:Gene Expression Regulation / Q000502:physiology*; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D018161:Receptors, Mineralocorticoid / Q000819:agonists / Q000378:metabolism*; D013148:Spironolactone / Q000494:pharmacology; D019311:Staurosporine / Q000633:toxicity; D014162:Transfection / Q000379:methods; D014316:Tritium / Q000493:pharmacokinetics
D004791:Enzyme Inhibitors; D000451:Mineralocorticoid Receptor Antagonists; D018161:Receptors, Mineralocorticoid; D014316:Tritium; D013148:Spironolactone; D000450:Aldosterone; D019311:Staurosporine