Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH.
A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.
Kumaravel
Tirukalikundram S
TS
Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Ontario, Canada.
Bristow
Robert G
RG
D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D018450:Disease Progression; D005260:Female; D016158:Genes, p53; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009153:Mutagens; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D014407:Tumor Cells, Cultured
D009153:Mutagens; D018719:Erb-b2 Receptor Tyrosine Kinases