[Spectrophotometric determination of albumin with acid brown SR].
A new method for the determination of albumin in human serum and mouse serum has been developed by spectrophotometry coupled with acid brown SR(ASR) as probe molecule. The maximum absorption wavelength of ASR was at 445 nm, while the maximum absorption wavelength of their product was at 610 nm. However, the reaction of ASR with albumin such as BSA or HSA was so strong that parts of their product were undissoluble in water. The addition of gum water into the system effectively eliminated the deposition. Under optimum reaction conditions, the ranges of working lines for BSA and HSA were 0-91.0 mg x L(-1) and 0-95.2 mg x L(-1), respectively. The detection limits were 5.72 mg x L(-1) for BSA and 5.15 mg x L(-1) for HSA. The relative standard derivation and the recovery of the method for the determination of total proteins in 6 human serum samples were 1.8%-4.4% and 93.6% - 109.1%, respectively. The proposed method has been employed in the assay of protein of human serum and mouse serum. The results of this work were in agreement with those obtained by Biuret method.
Zhao
Chang-Rong
CR
College of Chemistry and Environment Science, Hebei University, Baoding 071002, China.
Liu
Bao-Sheng
BS
Zhang
Hong-Yi
HY
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D015394:Molecular Structure; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry
D000596:Amino Acids; D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D012709:Serum Albumin; C549931:acid brown SR; D012710:Serum Albumin, Bovine