AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7.
In plant chloroplasts two superoxide dismutase (SOD) activities occur, FeSOD and Cu/ZnSOD, with reciprocal regulation in response to copper availability. This system presents a unique model to study the regulation of metal-cofactor delivery to an organelle. The Arabidopsis thaliana gene AtCCS encodes a functional homolog to yeast Ccs1p/Lys7p, a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. AtCCS mRNA expression levels are upregulated in response to Cu-feeding and senescence. We propose that AtCCS expression is regulated to allow the most optimal use of Cu for photosynthesis.
Abdel-Ghany
Salah E
SE
Department of Biology, Colorado State University, Anatomy/Zoology Building, Fort Collins, 80523, USA.
Burkhead
Jason L
JL
Gogolin
Kathryn A
KA
Andrés-Colás
Nuria
N
Bodecker
Jared R
JR
Puig
Sergi
S
Peñarrubia
Lola
L
Pilon
Marinus
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D008954:Models, Biological; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; C500748:CCS protein, Arabidopsis; C491960:CCS1 protein, S cerevisiae; D018832:Molecular Chaperones; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins