[Quantitative determination of total flavonoids in sea-buckthorn fruit juice by three wavelength spectrophotometry].
Numerous studies dealing with the quantitative determination of total flavonoids in sea-buckthorn fruit juice by spectrophotometry are presented. The flavonoids in sea-buckthorn fruit juice and aluminate produce stable complex whose absorption occurred at longer wavelength. To determine the total flavonoids in sea-buckthorn fruit juice by traditional spectrophotometry method, baseline shift and asymmetric absorption peak occurred on the absorption curve. Quantitative determination of flavonoids in sea-buckthorn fruit juice by three wavelength spectrophotometry method can eliminate the absorbance error caused interfering components in turbid solution and the scattering effect. Background changing with the concentration change and asymmetric absorption peak problems can also be solved. The regression equation of concentration vs deltaA was obtained: deltaA = - 0.00703 + 0.00048c with a relation coefficient gamma = 0.9991. The experimental results demostrate the total flavonoids concentrations in 0-800 microg x mL(-1) with deltaA obeying linear relation when the absorbance was measured at wavelength lambda1 = 495 nm, lambda2 = 415 nm and lambda3 = 368 nm. The recovery is 97.0%-101.0% and the coefficient of variation is 0.058% (n = 9). The method is more advantageous than tranditional spectrophotometry method.
Hui
Rui-hua
RH
Department of Chemistry, Anshan Normal University, Anshan 114005, China.
Hou
Dong-yan
DY
Guan
Chong-xin
CX
Liu
Xiao-yuan
XY
D000465:Algorithms; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000737:chemistry; D001628:Beverages; D002712:Chlorides / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D031281:Hippophae / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
D017607:Aluminum Compounds; D002712:Chlorides; D005419:Flavonoids; D000077410:Aluminum Chloride