title: The olive leaf extract exhibits antiviral activity against viral haemorrhagic septicaemia rhabdovirus (VHSV).
abstract: A commercial plant extract derived from olive tree leaf (Olea europaea) (LExt) and its major compound, oleuropein (Ole), inhibited the in vitro infectivity of the viral haemorrhagic septicaemia virus (VHSV), a salmonid rhabdovirus. Incubation of virus with LExt or Ole before infection reduced the viral infectivity to 10 and 30%, respectively. Furthermore, LExt drastically decreased VHSV titers and viral protein accumulation (virucidal effect) in a dose dependent manner when added to cell monolayers 36 h post-infection. On the other hand, both the LExt and Ole were able to inhibit cell-to-cell membrane fusion induced by VHSV in uninfected cells, suggesting interactions with viral envelope. Therefore, we propose that O. europaea could be used as a potential source of promising natural antivirals, which have demonstrated to lack impact on health and environment. In addition, Ole could be used to design other related antiviral agents.
authors.affiliation: Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, E-03202-Elche, Alicante, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Micol
authors.forename: Nuria
authors.initials: N
authors.lastname: Caturla
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Fons
authors.forename: Vicente
authors.initials: V
authors.lastname: Más
authors.forename: Luis
authors.initials: L
authors.lastname: Pérez
authors.forename: Amparo
authors.initials: A
authors.lastname: Estepa
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D008826:Microbial Sensitivity Tests; D029141:Novirhabdovirus / Q000187:drug effects* / Q000502:physiology; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts