High levels of HPV-16 DNA are associated with high-grade cervical lesions in women at risk or infected with HIV.
OBJECTIVE
To examine associations between levels of episomal and integrated human papillomavirus (HPV) 16 DNA and the grade of cervical disease.


METHODS
Cross-sectional data were obtained from a cohort of women with and without HIV infection and with high-risk sexual behaviour.


METHODS
Episomal and integrated HPV-16 DNA loads were measured in cervicovaginal lavages collected from 75 women (58 HIV seropositive, 17 HIV seronegative) using real-time polymerase chain reaction assays, controlling for cell content and the presence of inhibitors.


RESULTS
HPV-16 viral loads were significantly higher in women with high-grade squamous intraepithelial lesions (n = 6) than in women with normal cytology (n = 44), whether total (10(8.28) versus 10(5.10) HPV-16 DNA copies/microg DNA), episomal (10(7.99) versus 10(4.61)) or integrated (10(7.95) versus 10(4.77)) HPV-16 viral loads were measured (P < 0.02 for each comparison). Thirty-nine women had colposcopy [11 normal cervix, 16 cervical intraepithelial neoplasia (CIN) 1, six CIN 2, six CIN 3] and 24 additional women had three consecutive normal cytology smears. Controlling for age, race, CD4 cell count and HIV status, total (OR 3.5, 95% CI 1.2-10.4; P = 0.02), episomal (OR 2.9, 95% CI 1.2-7.4; P = 0.02,) and integrated (OR 1.6, 95% CI 1-2.6; P = 0.05) HPV-16 DNA loads were significantly associated with CIN 2,3, but the differences between CIN 1 and CIN 2,3 were not significant (P > 0.06). A greater amount of cellular DNA was collected from women with CIN 2,3 (P = 0.007).


CONCLUSIONS
Higher HPV-16 DNA loads are associated with cervical lesions detected by either histology or cytology. No additional information is gained by measuring integrated or episomal over total HPV-16 DNA loads.
Fontaine
Julie
J
Laboratoire de Virologie Moléculaire, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.
Hankins
Catherine
C
Mayrand
Marie-Hélène
MH
Lefevre
Jonas
J
Money
Deborah
D
Gagnon
Simon
S
Rachlis
Anita
A
Pourreaux
Karina
K
Ferenczy
Alex
A
Coutlée
François
F
D000328:Adult; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000821:virology; D003127:Colposcopy; D003430:Cross-Sectional Studies; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012309:Risk-Taking; D014412:Tumor Virus Infections / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014626:Vaginal Smears; D019562:Viral Load
D015344:DNA Probes, HPV; D004279:DNA, Viral