[Screening for phenanthrene-degrading bacteria and its characteristics].
One bacterial strain, designated as Phx1, was isolated from Daqing oil-field with the method of sublimate-plate and determination of dioxygenase activity. Phxl can degrade phenanthrene (100microg/mL, in LB media) completely in 24 h. Compared to MSM, LB and broth media can accelerate the phenanthrene degradation, promote the degradation of intermediates especially. With the method of plasmid elimination, it was proved that the genes encoding for degradation of phenanthrene locate on the larger one of the two plasmids. According to morphological and physio-biochemical characteristics, Phxl was identified as Agrobacterium sp. and was probably a new species of the genus Agrobacterium due to its 16S rDNA sequence which has the similarities of 97.8 % and 96.6% to Agrobacterium tumefaciens UP-3 and Agrobacterium albertimagni respectively.
Zhang
Lei
L
Key laboratory of Agro-Microbial Resource and Application, College of Biological Science, China Agricultural University, Beijing 100094, China.
Yuan
Hong-li
HL
D001673:Biodegradation, Environmental; D010616:Phenanthrenes / Q000378:metabolism*; D012231:Rhizobium / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene