[Screening and identification of a strain for hydroxylation of nicotinic acid].
Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.
Lu
Wei-hong
WH
Jiangsu Key Laboratory for Bioresource technology, NNU Laboratory of Microbiology and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. luweihong168@sohu.com
Xu
Li
L
Dai
Yi-jun
YJ
Yuan
Sheng
S
D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000235:genetics; D006900:Hydroxylation; D019787:Linoleic Acid / Q000378:metabolism*; D010802:Phylogeny; D016958:Pseudomonas putida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019787:Linoleic Acid