In vitro and in vivo suppression of GJB2 expression by RNA interference.
Mutations in GJB2 (gap junction protein, beta-2) are the major cause of autosomal recessive non-syndromic hearing loss. A few allele variants of this gene also cause autosomal dominant non-syndromic hearing loss as a dominant-negative consequence of expression of the mutant protein. Allele-specific gene suppression by RNA interference (RNAi) is a potentially attractive strategy to prevent hearing loss caused by this mechanism. In this proof-of-principle study, we identified a potent GJB2-targeting short interfering RNA (siRNA) to post-transcriptionally silence the expression of the R75W allele variant of GJB2 in cultured mammalian cells. In a mouse model, this siRNA duplex selectively suppressed GJB2(R75W) expression by >70% of control levels, thereby preventing hearing loss. The level of endogenous murine Gjb2 expression was not affected. Our data show that RNAi can be used with specificity and efficiency in vivo to protect against hearing loss caused as a dominant-negative consequence of mutant gene expression.
Maeda
Yukihide
Y
Molecular Otolaryngology Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Interdepartmental Ph.D. Genetics Program, The University of Iowa, 200 Hawkins Drive-21151 PFP, Iowa City, IA 52242, USA.
Fukushima
Kunihiro
K
Nishizaki
Kazunori
K
Smith
Richard J H
RJ
D000818:Animals; D001483:Base Sequence; D000072259:Connexin 26; D017630:Connexins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005799:Genes, Dominant; D034381:Hearing Loss / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D034622:RNA Interference; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D017630:Connexins; C000607042:GJB2 protein, human; C000607043:Gjb2 protein, mouse; D000072259:Connexin 26