title: An N-terminal region of translationally controlled tumor protein is required for its antiapoptotic activity.
abstract: Bcl-xL plays a critical role in maintaining cell survival. However, the relationship between the potential interaction of Bcl-xL with other cytosolic proteins and the regulation of cell survival remains incompletely defined. We have identified translationally controlled tumor protein (TCTP), a multifunctional protein, as a novel antiapoptotic Bcl-xL-interacting protein. TCTP interacted in vivo and in vitro with Bcl-xL, and their sites have been mapped to an N-terminal region of TCTP and the Bcl-2 homology domain 3 of Bcl-xL. Consistent with a role in maintaining T-cell survival during activation, TCTP was significantly upregulated in murine T cells activated by T-cell antigen receptor (TCR) ligation and CD28 costimulation, which was correlated with the upregulation of Bcl-xL in activated T cells. Moreover, downregulation of TCTP expression by antisense technology in T cells results in the increase of T-cell apoptosis. Furthermore, the N-terminal region of TCTP was required for its ability to inhibit apoptosis. In conclusion, this study has demonstrated that an N-terminal region of a cytosolic protein, TCTP, is required for its binding to Bcl-xL and for its antiapoptotic activity.
authors.affiliation: Department of Medicine, Laboratory of Immunopathology, Biology of Inflammation Center, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Xiong
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Xinmen
authors.initials: X
authors.lastname: Wang
authors.forename: Michiya
authors.initials: M
authors.lastname: Nishino
authors.forename: Dragan
authors.initials: D
authors.lastname: Mirkovic
authors.forename: Justin
authors.initials: J
authors.lastname: Nguyen
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D000090324:Tumor Protein, Translationally-Controlled 1; D015854:Up-Regulation; D051020:bcl-X Protein
chemical_list: C494811:BCL2L1 protein, human; C494812:Bcl2l1 protein, mouse; D014408:Biomarkers, Tumor; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1; D051020:bcl-X Protein