title: Preparation of red blood cell column for capillary electrochromatography.
abstract: A column packed with red blood cells (RBCs) was prepared for electrochromatography as a separation and reaction column. RBCs were kept inside a piece of fused silica capillary tubing with 2% agarose gel. In the column, RBCs were uniformly distributed in the agarose gel matrix and their electrophoretic movements due to an applied voltage were suppressed well. The durability of the biological function of the column under applied voltage was about 1 h, although it could remain for 2-3 days without applied voltage. The column could not be used when hemolysis of the RBCs was observed in the column. When the developed "RBC-gel column" was used, both pyridoxamine and serotonin were converted to other compounds through their direct contact with RBCs.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Sakaguchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kato
authors.forename: Asami
authors.initials: A
authors.lastname: Ito
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuda
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D019075:Electrophoresis, Capillary; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D011733:Pyridoxamine / Q000097:blood; D012701:Serotonin / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012701:Serotonin; D011733:Pyridoxamine