title: Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro.
abstract: ProRoot Mineral Trioxide Aggregate (MTA) has been indicated as a pulp capping material. The purpose of this study was to evaluate the effect of tooth-colored (white) MTA on pulp cell apoptosis and cell cycle. Mouse odontoblast-like cells (MDPC-23) and undifferentiated pulp cells (OD-21) were exposed to 0 to 100 mg MTA for 24 h. Propidium iodide staining followed by flow cytometry demonstrated that MTA did not induce apoptosis of MDPC-23 or OD-21 (p > 0.05). Cell cycle analysis showed that MTA induced a modest (but significant) increase in the percentage of MDPC-23 in the S and G2 phases, and OD-21 in the S phase of cell cycle, as compared to untreated controls (p </= 0.05). In conclusion, MTA induced proliferation, and not apoptosis, of pulp cells in vitro. These findings suggest a potential mechanism to explain the regenerative effect observed in the dentin-pulp complex when MTA was used for direct pulp capping.
authors.affiliation: Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI 48109-1078, USA.
authors.forename: Sasan
authors.initials: S
authors.lastname: Moghaddame-Jafari
authors.forename: Maria G
authors.initials: MG
authors.lastname: Mantellini
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Botero
authors.forename: Neville J
authors.initials: NJ
authors.lastname: McDonald
authors.forename: Jacques E
authors.initials: JE
authors.lastname: Nör
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003785:Dental Pulp Capping; D004338:Drug Combinations; D005434:Flow Cytometry; D008422:Materials Testing; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate