Taq-I polymorphism in 3'UTR of the IL-12B and association with IL-12p40 production from human PBMC.
A wide array of studies has demonstrated differences in genotype and allele frequencies of cytokine gene polymorphisms depending on ethnicity and race. In this study, the frequency of Taq-I polymorphism in 3' untranslated region of IL-12B was investigated in two Bulgarian ethnic groups-Bulgarians and Turkish minority. No significant differences of genotype and allele frequencies were observed between these groups. Genotype distribution in the total group of Bulgarian citizens was: AA (61%), CA (32%) and CC (7%), and the allele frequency of 16974 A allele was 0.77. We also evaluated whether this polymorphism affects IL-12p40 production from human PBMC after stimulation. We demonstrated that association between genotype and IL-12p40 production by stimulated PBMC depends on the stimuli used. Our results indicated a significantly decreased IL-12 p40 secretion for the following order of genotypes: AA>CA>CC, after stimulation of PBMC with C3-binding glycoprotein (C3bgp) in contrast to lipopolysaccharide, phytohaemagglutinin and pokeweed mitogen.
Stanilova
S
S
Department of Molecular Biology, Immunology and Genetics, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria. stanilova@mf.uni-sz.bg
Miteva
L
L
D020413:3' Untranslated Regions / Q000235:genetics*; D000483:Alleles; D002478:Cells, Cultured; D005787:Gene Frequency; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics* / Q000378:metabolism*; D053762:Interleukin-12 Subunit p40; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D021122:Protein Subunits; D018664:Interleukin-12