[Stimulation of HCG, estrogen and progesterone production in isolated trophoblast cells by glycodelin A or its N-glycans].
The immunosuppressive protein glycodelin A (GdA) is secreted by decidual tissue in high rates in the first trimester of pregnancy. GdA forms about 10 % of the total protein amount released by the decidua in the first trimester of pregnancy and has a unique glycosylation including fucosylated LacdiNAc structures. The purpose of this study was to investigate the effect of glycodelin A and its N-glycans on hCG, estrogen and progesterone release by cytotrophoblasts in vitro. Trophoblast cells were isolated from human term placenta by fragmentation of villous tissue. Trophoblast cells fuse in vitro to syncytiotrophoblast cells. Trophoblast cells were incubated with GdA and various glycans of GdA. The production of hCG and progesterone was measured after 24 and 48 hours. The production of hCG, estrogen and progesterone was increased in GdA and glycan-treated cell cultures as compared to untreated trophoblast cell cultures.


CONCLUSIONS
HCG, estrogen and progesterone are markers for the differentiation process of cytotrophoblast cells. The results suggest that GdA with its distinct glycosylation modulates the differentiation of trophoblasts.
Jeschke
U
U
Ludwig Maximilians Universität München, I. Frauenklinik-Innenstadt, Maistrasse 11, 80337 München. udo.jeschke@med.uni-muenchen.de
Richter
D-U
DU
Briese
V
V
Mylonas
I
I
Friese
K
K
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D010920:Placenta / Q000166:cytology* / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D004967:Estrogens; D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011134:Polysaccharides; D011257:Pregnancy Proteins; D011374:Progesterone