title: Genetic environment and expression of the extended-spectrum beta-lactamase blaPER-1 gene in gram-negative bacteria.
abstract: The genetic location of the gene coding for the expanded-spectrum beta-lactamase PER-1 was analyzed in a series of gram-negative isolates. It was identified as part of a composite transposon bracketed by two novel insertion elements, ISPa12 and ISPa13, belonging to the IS4 family that possess transposases that share 63% amino acid identity and that are chromosomally located in Pseudomonas aeruginosa, Providencia stuartii, and Acinetobacter baumannii. On the contrary, the bla(PER-1) gene was identified just downstream of an ISPa12 element but not within a composite transposon when it was located on a plasmid in Salmonella enterica serovar Typhimurium and A. baumannii isolates. In both cases, expression of the bla(PER-1) gene was driven by promoter sequences located in ISPa12.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Université Paris XI, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Ludovic
authors.initials: L
authors.lastname: Cabanne
authors.forename: Haluk
authors.initials: H
authors.lastname: Vahaboglu
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D018274:Electroporation; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005838:Genotype; D006090:Gram-Negative Bacteria / Q000235:genetics*; D032023:Integrons / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D001618:beta-Lactamases