[Roles of mitochondria in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells].
OBJECTIVE
In order to investigate the roles of mitochondria in vitamin E succinate (VES)-induced apoptosis in human gastric cancer SGC-7901 cells.


METHODS
Mitochondrial transmembrane potential (deltapsi(m)) was observed by confocal laser scanning microscopy, while the expression of cytochrome c in cytosol and caspase-3 was measured by western blotting after the cells were treated with VES at 5, 10, 20 microg/ml.


RESULTS
VES obviously decreased deltapsi(m) with dose- and time-dependent relationship, increased the expression of cytochrome c in cytosol and caspase-3 and activated caspase-3 as well.


CONCLUSIONS
VES-induced apoptosis in SGC-7901 cells might involve mitochondrial permeability transition, release of cytochrome c and activation of caspase-3 downstream.
Wu
Kun
K
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150001, China.
Li
Gui-Chang
GC
Zhao
Yan
Y
Zhang
Xiao-Hua
XH
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology; D013274:Stomach Neoplasms / Q000473:pathology*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
D014810:Vitamin E; D045304:Cytochromes c; D053148:Caspase 3