title: Expression of myelencephalon-specific protease after cryogenic lesioning of the rat parietal cortex.
abstract: The gene for myelencephalon-specific protease (MSP) is a member of the kallikrein gene family and in rats is expressed mainly in the central nervous system. Its function and alteration in brain injury have not yet been clarified. We examined the expression of MSP after cryogenic injury (CI) using in situ hybridization, immunohistochemistry, and Western blotting. Analysis of MSP mRNA by in situ hybridization revealed a higher level of expression around the cryogenic area than on the contralateral side at 2-7 days after CI, with peak expression occurring 7 days after CI. Immunohistochemical analysis demonstrated expression of MSP protein at 1 day after CI, in the same region in which MSP mRNA was observed, with peak expression again at 7 days after CI, in the area around the lesion. Double immunohistochemical labeling revealed that MSP was expressed mainly in oligodendrocytes. These results suggest that expression of MSP may be related to the turnover of myelin-associated proteins and extracellular matrix proteins after CI. The regulation of active MSP may be important in the physiological or pathological changes involved in remyelination or demyelination.
authors.affiliation: Department of Neurosurgery and Restorative Neuroscience, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Muzuho-ku, Nagoya 467-8601, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Uchida
authors.forename: Mineyoshi
authors.initials: M
authors.lastname: Aoyama
authors.forename: Masataka
authors.initials: M
authors.lastname: Fujita
authors.forename: Naokazu
authors.initials: N
authors.lastname: Hotta
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Tada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katano
authors.forename: Mitsuhito
authors.initials: M
authors.lastname: Mase
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Asai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D004195:Disease Models, Animal; D018450:Disease Progression; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005615:Freezing; D007150:Immunohistochemistry; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism; D009413:Nerve Fibers, Myelinated / Q000201:enzymology* / Q000473:pathology; D009836:Oligodendroglia / Q000201:enzymology*; D010296:Parietal Lobe / Q000201:enzymology* / Q000293:injuries* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D012697:Serine Endopeptidases; C108816:myelencephalon-specific serine protease