title: Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine.
abstract: The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12 mM ammonium acetate and 87.6 mM acetic acid in methanol-acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3 min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24 microg L(-1) for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett-Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C18 cartridge was necessary. Real determination of these analytes in two patient urines were done.
authors.affiliation: Department of Analytical Chemistry and Foods Technology, University de Castilla-La Mancha, Avenida Camilo Jose Cela 10, Ciudad Real 13071, Spain. juana.rflores@uclm.es
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Flores
authors.forename: J J
authors.initials: JJ
authors.lastname: Berzas Nevado
authors.forename: A M
authors.initials: AM
authors.lastname: Contento Salcedo
authors.forename: M P
authors.initials: MP
authors.lastname: Cabello Díaz
mesh_terms: D000970:Antineoplastic Agents / Q000652:urine*; D001549:Benzamides; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000652:urine*; D011743:Pyrimidines / Q000652:urine*; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate