title: Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques.
abstract: Theresponse of Corynebacterium glutamicum to ammonium limitation was studied by transcriptional and proteome profiling of cells grown in a chemostat. Our results show that ammonium-limited growth of C. glutamicum results in a rearrangement of the cellular transport capacity, changes in metabolic pathways for nitrogen assimilation, amino acid biosynthesis, and carbon metabolism, as well as a decreased cell division. Since transcription at different growth rates was studied, it was possible to distinguish specific responses to ammonium limitation and more general, growth rate-dependent alterations in gene expression. The latter include a number of genes encoding ribosomal proteins and genes for F(o)F(1)-ATP synthase subunits.
authors.affiliation: Institut für Biochemie, Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Silberbach
authors.forename: Mathias
authors.initials: M
authors.lastname: Schäfer
authors.forename: Andrea T
authors.initials: AT
authors.lastname: Hüser
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Reinhard
authors.initials: R
authors.lastname: Krämer
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D000222:Adaptation, Physiological; D000596:Amino Acids / Q000096:biosynthesis; D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D004734:Energy Metabolism; D009584:Nitrogen / Q000378:metabolism; D020543:Proteome; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D020543:Proteome; D000644:Quaternary Ammonium Compounds; D002244:Carbon; D009584:Nitrogen