Development and use of an internal positive control for detection of Bordetella pertussis by PCR.
An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.
Herwegh
Stéphanie
S
Laboratoire de Bactériologie-Hygiène, Hôpital A. Calmette, F-59037-Lille Cedex, France.
Carnoy
Christophe
C
Wallet
Frédéric
F
Loïez
Caroline
C
Courcol
René J
RJ
D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial