[The effect of pcDNA 3.1/RPs15 on skin fibroblasts in vitro].
OBJECTIVE
To construct eukaryotic expression vector of ribosomal protein sl5(RPs15) gene and study its effect on mouse skin fibroblasts in vitro.


METHODS
The RPs15 cDNA encoding region of fetal mouse skin was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method and cloned into eukaryotic expression vector pcDNA3.1(-). The recombinant plasmid was transfected into adult mouse skin fibroblasts by FuGENE6 transfection reagents. Then the expression of RPs15 gene, was detected and its biological effect on fibroblasts was measured.


RESULTS
The DNA sequencing result of pcDNA3.1/RPs15 was identical with the reported. The RPs15 gene was expressed in transfected fibroblasts. The growth density of fibroblasts decreased with the conformation changing accordingly.


CONCLUSIONS
The eukaryotic expression vector pcDNA3.1/RPs15 is successively constructed and can be expressed in mouse skin fibroblasts. The results set up a basis for further study of the effect of RPs15 gene on skin fibroblasts.
Tang
Sheng-jian
SJ
Institute of Plastic Surgery, Weifang Medical University, Weifang 261042, China.
Wang
Xiao-ke
XK
LV
Xiao-jie
XJ
Liu
Xia
X
Zhang
Peng
P
Zhang
Jun
J
Zhou
Xiao-yan
XY
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005822:Genetic Vectors; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D012269:Ribosomal Proteins; D012867:Skin / Q000166:cytology; D014162:Transfection
D018076:DNA, Complementary; D009687:Nuclear Proteins; D012269:Ribosomal Proteins; C069798:Rps15 protein, mouse