title: Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach.
abstract: OBJECTIVE
  To study multiple gene expression patterns and their roles in the process of gastric carcinogenesis.
  
  
  RESULTS
  Using a high-throughput tissue microarray technique, 169 specimens from gastric carcinomas, precursor lesions and normal mucosa were immunostained on a series of tissue chips for p53, p21(WAF1/CIP1) cyclin E, Bcl-2, c-met and mucin 5AC expression. The overexpression of p53 was observed in 10.7% of low-grade dysplasia (LGD), 38.1% of high-grade dysplasia (HGD) and 39.6% of intestinal type gastric carcinoma (IGC). Expression of p21(WAF1/CIP1) was found in 47.6% of incomplete intestinal metaplasia (IM), 36.7% of dysplasia (Dys) and 29.5% of IGC. The overexpression of cyclin E was more frequently present in carcinomas than in Dys (P < 0.05); moreover, high-level expression (> 25% in extent) of cyclin E was observed only among IGC. Abnormal Bcl-2 expression was present in 81.0% of incomplete IM, 69.4% of Dys and 22.9% of IGC. Along with progression of the lesion, the expression of c-met increased; in contrast, mucin 5AC decreased gradually.
  
  
  CONCLUSIONS
  The specific expression pattern in incomplete IM was mucin 5AC+/Bcl-2+/p53-/cyclin E-, while mucin 5AC-/cyclin E+ was specific for IGC. p53 was useful for distinguishing LGD from HGD. High-level expression of cyclin E might be an indicator for malignant transformation of dysplasia.
authors.affiliation: Department of Pathology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Peking University School of Oncology, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: J-Y
authors.initials: JY
authors.lastname: Li
authors.forename: J-S
authors.initials: JS
authors.lastname: He
authors.forename: L-X
authors.initials: LX
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Chen
mesh_terms: D018797:Cell Cycle Proteins / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D050759:Cyclin-Dependent Kinase Inhibitor p21; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007422:Intestines / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D055271:Mucin 5AC; D009077:Mucins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D019859:Proto-Oncogene Proteins c-met / Q000032:analysis; D013270:Stomach / Q000737:chemistry / Q000473:pathology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D046888:Tissue Array Analysis / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: C493956:CDKN1A protein, human; D018797:Cell Cycle Proteins; D019927:Cyclin E; D050759:Cyclin-Dependent Kinase Inhibitor p21; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D009077:Mucins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D019859:Proto-Oncogene Proteins c-met