title: Biotransformation and impact of ferulic acid on phenylpropanoid and capsaicin levels in Capsicum annuum L. cv. P1482 cell suspension cultures.
abstract: Cell suspension cultures of Capsicum annuum L. cv. P1482 were fed with exogenous ferulic acid to monitor their biotransformation abilities. A portion of the ferulic acid was biotransformed into vanillin, a major natural flavor, and capsaicin, a principle secondary metabolite characteristic of Capsicum species. The cellular vanillin concentrations were relatively higher than capsaicin levels and were maximal (2 mg/g DW) 4 days after 0.6 mM ferulic acid feeding. Maximal vanillin levels in the culture medium were 10 mg/L at 4 and 3 days after feeding with 1.25 and 2.5 mM ferulic acid, respectively. With regard to capsaicin levels, the cellular levels were slightly decreased by ferulic acid feeding, whereas the levels in the culture medium were increased. Ferulic acid feeding not only enhanced vanillin and capsaicin production but also increased the concentrations of other phenylpropanoid metabolites.
authors.affiliation: Divisions of Forest Science, Gyeongsang National University, Jinju, Korea.
authors.forename: Seung-Mi
authors.initials: SM
authors.lastname: Kang
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Kang
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Min
authors.forename: C S
authors.initials: CS
authors.lastname: Karigar
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Yang
authors.forename: Sun-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Yeong-Rae
authors.initials: YR
authors.lastname: Ha
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Choi
mesh_terms: D001547:Benzaldehydes / Q000032:analysis* / Q000378:metabolism; D001711:Biotransformation; D002211:Capsaicin / Q000032:analysis* / Q000378:metabolism; D002212:Capsicum / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D003373:Coumaric Acids / Q000008:administration & dosage* / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D003373:Coumaric Acids; C004999:ferulic acid; C100058:vanillin; D002211:Capsaicin