Biological effect of irradiation on adhesion molecules in human colon cancer cells in vitro.
BACKGROUND
Carbohydrate antigens, such as sialyl Lewis(a) antigen (s-Le(a)) and sialyl Lewis(x) antigen (s-Le(x)), play an important role in cancer metastasis in vitro and in vivo. Currently, preoperative radiotherapy is used to prevent local recurrence of rectal cancer. We investigated the effects of X-ray irradiation on the carbohydrate antigens s-Le(a) and s-Le(x) in vitro.


METHODS
The cell surface expressions of s-Le(a) and s-Le(x) were determined by flow cytometric analysis at 24 hours after X-ray irradiation of 4 human cancer cell lines. s-Le(a) and s-Le(x) functions were quantitated using a monolayer cell adhesion assay with human umbilical vein endothelial cells (HUVECs).


RESULTS
The cell surface expressions of s-Le(a) and s-Le(x) decreased at 24 hours after irradiation. s-Le(a) adhesion to HUVECs monolayers similarly decreased at 24 hours after irradiation.


CONCLUSIONS
These results may indicate a role for X-ray irradiation in the reduction of liver metastasis in patients with colon cancer.
Hasegawa
Takeshi
T
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hasetake@med.nagoya-cu.ac.jp
Sato
Mikinori
M
Kurimoto
Masaaki
M
Takahashi
Hiroki
H
Kawashima
Takashi
T
Matsuo
Youichi
Y
Yamamoto
Minoru
M
Sawai
Hirozumi
H
Funahashi
Hitoshi
H
Okada
Yuji
Y
Takeyama
Hiromitsu
H
Manabe
Tadao
T
D018395:CA-19-9 Antigen; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy*; D042783:Endothelial Cells / Q000166:cytology; D005434:Flow Cytometry; D005732:Gangliosides / Q000096:biosynthesis / Q000502:physiology / Q000528:radiation effects*; D019073:HT29 Cells; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis / Q000502:physiology / Q000528:radiation effects*; D000080506:Sialyl Lewis X Antigen
D018395:CA-19-9 Antigen; D005732:Gangliosides; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C064564:sialyl Le(a) ganglioside