[Construction of an expression vector under the control of hTERT promoter].
OBJECTIVE
To construct a luciferase expression vector driven by hTERT(human telomerase reverse transcriptase) core promoter and identify the transcriptional activity of the vector in tumor cells and normal cells.


METHODS
hTERT gene core promoter was amplified by PCR using the total genomic DNA from HeLa cells as template. The amplified gene fragment was subsequently cloned into PGL3-basic vector. Then the expression vector was confirmed by restriction enzyme digestion and PCR analysis. The luciferase activity driven by the hTERT gene core promoter was identified after transient transfection of the expression vector into tumor cells and normal cells.


RESULTS
The luciferase activity was high in the transfected tumor cells, and very low in transfected normal cells.


CONCLUSIONS
hTERT gene core promoter is tumor-specific and may be useful in gene therapy of tumor.
Han
Ming-kun
MK
Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. 20012020@sina.com
Zhang
Hui-zhong
HZ
Cheng
Shi-yin
SY
D004262:DNA Restriction Enzymes / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
D008156:Luciferases; C509186:TERT protein, human; D019098:Telomerase; D004262:DNA Restriction Enzymes