Regulation of IL-18 expression by CRH in mouse microglial cells.
Corticotropin releasing hormone (CRH) is a major regulator of the stress response. This study examined whether CRH regulates interleukin-18 expression on microglia, BV2. Our data show that CRH enhanced IL-18 expression and significantly induced the secretion of functional IL-18 protein. Furthermore, CRH induced IL-18 production could be blocked by N-acetyl-L-cystein (NAC), which suggests that reactive oxygen intermediates (ROI) may be involved in regulating IL-18. Indeed, it was also found that CRH increased the generation of ROI. Taken together, these results indicate that CRH is an important mediator that regulates IL-18 expression in the brain during stress.
Yang
Yoolhee
Y
Department of Life science, Sookmyung Women's University, Seoul, South Korea.
Hahm
Eunsil
E
Kim
Youngin
Y
Kang
Jaeseung
J
Lee
Wangjae
W
Han
Innoc
I
Myung
Pyungkeun
P
Kang
Hyungsik
H
Park
Hyunjeong
H
Cho
Daeho
D
D000818:Animals; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D017186:Titrimetry
D020382:Interleukin-18; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D018019:Receptors, Corticotropin-Releasing Hormone; D007371:Interferon-gamma; D003346:Corticotropin-Releasing Hormone