Low-level arsenite activates the transcription of genes involved in adipose differentiation.
In this study we analyzed gene expression in 3T3-F442A pre-adipocyte cells that differentiate in the presence of micro-molar arsenate concentration. Two concentrations of arsenite (As2O3, 0.25 micromol/L and 0.5 micromol/L) were applied for three days with and without insulin (170 nmol/L) and gene expressions were evaluated by quantitative RT-PCR. The genes included genes of oxidative-stress responses: heme-oxygenase-1 (HO1) and the hypoxia inducible factor 1a (HIF1alpha), genes of cell-cycle: c-jun and Kruppel like factor 5 (KLF5), and genes that play important roles in adipose determination: a peroxisome proliferator-activated receptor (PPARgamma) and a CCAAT/ enhancer binding protein (C/EBPalpha). Arsenite induced the expression of HO1, HIF1alpha, KLF5, PPARgamma and C/EBPalpha. These results suggest that under condition of oxidative stress arsenite induces genes that are required for adipose differentiation.
Salazard
B
B
Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, (EA 1784, IFR112, PMSE), Faculté de Médecine, Marseille, France.
Bellon
L
L
Jean
S
S
Maraninchi
M
M
El-Yazidi
C
C
Orsière
T
T
Margotat
A
A
Botta
A
A
Bergé-Lefranc
J-L
JL
D016475:3T3 Cells; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D018053:Arsenites / Q000494:pharmacology*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D051547:Heme Oxygenase-1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013723:Teratogens / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D018053:Arsenites; D022763:CCAAT-Enhancer-Binding Protein-alpha; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C118391:Klf5 protein, mouse; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins; D047495:PPAR gamma; D013723:Teratogens; D015534:Trans-Activators; D014157:Transcription Factors; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; C015001:arsenite