title: The role of thrombofilia related to Factor V Leiden and Factor II G20210A mutations in recurrent abortions.
abstract: OBJECTIVE
  To study, the impact of thrombophilia caused by Factor V, Factor ll G20210A mutations on recurrent abortions, the prevelance of Factor V Leiden and Factor II G20210A mutation in patients with habitual abortions.
  
  
  METHODS
  Forty one patients with a diagnosis of habitual abortion were enrolled in the study. Control group consisted of 50 women without a history of poor obstetric outcome. 10 ml. peripheral venous blood was taken from study and control groups and transferred to EDTA tubes, and were tested for Factor V Leiden and Factor II G20210A mutations by PCR in genetics laboratory.
  
  
  RESULTS
  Phenotypes of 91 cases were determined by PCR for Factor V Leiden. It was established that of 41 patients in the study group, 31 (75.6%) had GG genotype, 9 (22%) had GA genotype and 1 (2.4%) had AA genotype. In the control group, 45 (90%) of 50 women had GG genotype and 5 (10%) had GA genotype. A allele carrier status was found to be 24.4% in study group and 10% in the control group. The difference between them was not statistically significant (p = 0.06).
  
  
  CONCLUSIONS
  The results obtained from patients and control group have no difference in Factor V Leiden and Factor II G20210A mutations.These results suggest that mutations have no role in etiology of 1. and 2. trimester recurrent abortions.
authors.affiliation: Department of Obstetrics and Gynecology, Ankara University School of Medicine Cebeci, Ankara, Turkey.
authors.forename: Muge
authors.initials: M
authors.lastname: Aksoy
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Tek
authors.forename: Halil
authors.initials: H
authors.lastname: Karabulut
authors.forename: Bulent
authors.initials: B
authors.lastname: Berker
authors.forename: Feride
authors.initials: F
authors.lastname: Soylemez
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005165:Factor V / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D010154:Pakistan / Q000453:epidemiology; D010641:Phenotype; D021041:Placental Circulation / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D018566:Pregnancy, High-Risk / Q000235:genetics; D015995:Prevalence; D011516:Prothrombin / Q000235:genetics*; D012307:Risk Factors; D019851:Thrombophilia / Q000150:complications* / Q000453:epidemiology / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin