Gene cloning, expression and partial characterization of cell division protein FtsZ1 from extremely halophilic archaeon Haloarcula japonica strain TR-1.
The gene encoding a cell division protein FtsZ1 was cloned from an extremely halophilic archaeon, Haloarcula japonica strain TR-1. Nucleotide sequencing analysis of the ftsZ1 gene revealed that the structural gene consisted of an open reading frame of 1,158 nucleotides encoding 386 amino acids. Transcription of the ftsZ1 gene in Ha. japonica was confirmed by RT-PCR. A modified ftsZ1 gene was inserted into the shuttle vector pWL102 and used to transform Ha. japonica. The recombinant FtsZ1 was produced as a fusion with hexahistidine-tag in Ha. japonica host cells and purified. Purified recombinant FtsZ1 exhibited GTP-dependent polymerization activity and GTP-hydrolyzing activity in the presence of high concentrations of KCl.
Ozawa
Kazumichi
K
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Harashina
Takeyori
T
Yatsunami
Rie
R
Nakamura
Satoshi
S
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002455:Cell Division; D003001:Cloning, Molecular; D020558:GTP Phosphohydrolases / Q000378:metabolism; D015870:Gene Expression; D019613:Haloarcula / Q000187:drug effects / Q000235:genetics*; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011189:Potassium Chloride / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics
D019843:Archaeal Proteins; C119586:FtsZ protein, Methanococcus jannaschii; D012965:Sodium Chloride; D006639:Histidine; D011189:Potassium Chloride; D020558:GTP Phosphohydrolases