[Application of SensiScript enzyme to a RT-PCR system for the obtention of RNA of HIV-1 starting serum samples stored at -20 degrees C during a decade].
The efficiency of SensiScript reverse transciptase to obtain useful genetic material in the sequencing of the nucleic acid from HIV-1, starting from sera collected between 1989 and 1998 and kept at suboptimal temperatures, was proved. On using the SensiScript enzyme it was obtained an amplification of the RNA of the HIV-1 in 86.5 % of the studied samples, compared with 20 % on using the AMV-RT enzyme . No amplification was obtained in 13.5 % of the studied samples with any of the 2 enzymes used.
Aguirre
Sonia Resik
SR
Instituto de Medicina Tropical "Pedro Kouri" Ciudad de La Habana, Cuba. sresik@ipk.sld.cu
Ping
Li-Hua
LH
Barrios Olivera
Julio A
JA
Cardellá
Vivian Kourí
VK
Swanstrom
Ronald
R
D001769:Blood / Q000821:virology; D015925:Cryopreservation; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D012367:RNA, Viral