Developmental-stage-specific assembly of ParB complexes in Streptomyces coelicolor hyphae.
In Streptomyces coelicolor ParB is required for accurate chromosome partitioning during sporulation. Using a functional ParB-enhanced green fluorescent protein fusion, we observed bright tip-associated foci and other weaker, irregular foci in S. coelicolor vegetative hyphae. In contrast, in aerial hyphae regularly spaced bright foci accompanied sporulation-associated chromosome condensation and septation.
Jakimowicz
Dagmara
D
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom. dagmara.jakimowicz@bbsrc.ac.uk
Gust
Bertolt
B
Zakrzewska-Czerwinska
Jolanta
J
Chater
Keith F
KF
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics; D019077:Helix-Turn-Helix Motifs; D025301:Hyphae / Q000502:physiology*; D025282:Mycelium / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013171:Spores, Bacterial / Q000502:physiology; D048372:Streptomyces coelicolor / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C052883:chromosome partition proteins, bacterial; D004815:Epidermal Growth Factor