The presence of the cag pathogenicity island is associated with increased superoxide anion radical scavenging activity by Helicobacter pylori.
Reactive oxygen species (ROS) generated by Helicobacter pylori infection have been suggested to be important factors in induction of gastric malignancies. Utilizing electron spin resonance spectrometry, H. pylori-dependent radical formation and hydroxyl- and superoxide-anion radical scavenging activity was investigated. In contrast to previous reports, we found that H. pylori does not produce ROS, but displays superoxide scavenging activity. This scavenging activity was increased in cag-positive H. pylori strains when compared to strains lacking an intact cag pathogenicity island, and was dependent on enzyme activity. We hypothesize that the increased scavenging activity of cag-positive H. pylori strains is an adaptation to the increased inflammatory response associated with the cag-positive genotype of H. pylori.
Briedé
Jacob J
JJ
Department of Health Risk Analysis and Toxicology, Faculty of Health Sciences, Maastricht University, Maastricht, The Netherlands.
Pot
Raymond G J
RG
Kuipers
Ernst J
EJ
van Vliet
Arnoud H M
AH
Kleinjans
Jos C S
JC
Kusters
Johannes G
JG
D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D004578:Electron Spin Resonance Spectroscopy; D044404:Genomic Islands; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D017382:Reactive Oxygen Species; C080866:cagA protein, Helicobacter pylori; D013481:Superoxides