title: Effect of iontophoresis on skin permeation of defibrase.
abstract: OBJECTIVE
  To investigate the effect of iontophoresis on skin permeation of defibrase.
  
  
  METHODS
  Iontophoresis was carried out in side-by-side chambers, excised rat skin membrane (RSM) or human epidermis membrane (HEM). The effects of electrode polarity, permeation medium pH and ionic strength were evaluated.
  
  
  RESULTS
  Permeation of defibrase caused by anodal iontophoresis was more effective [the apparent permeability coefficient was (1.2 +/- 0.4) x 10(-4) cm x h(-1)] than that of cathodal iontophoresis [(4.3 +/- 1.4) x 10(-5) cm x h(-1)]. The amount of permeated defibrase caused by anodal iontophoresis in pH 7.4 medium was (25 +/- 5) x 10(-14) mol x cm(-2), which was higher than that of in pH 6. 4 permeation medium [(15 +/- 4) x 10(-14) mol x cm(-2)].
  
  
  CONCLUSIONS
  Iontophoresis could enhance skin permeation of defibrase. Electroosmotic flow effect played an important role.
authors.affiliation: School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. hyzhao@mail.buct.edu.cn
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Zhao
authors.forename: Qi-peng
authors.initials: QP
authors.lastname: Yuan
authors.forename: Jun-min
authors.initials: JM
authors.lastname: Zheng
mesh_terms: D000818:Animals; D012103:Batroxobin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004817:Epidermis / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007478:Iontophoresis; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption
chemical_list: D005343:Fibrinolytic Agents; D012103:Batroxobin