title: Recombinant chicken IL-6 does not activate heterophils isolated from day-old chickens in vitro.
abstract: Pro-inflammatory cytokines are produced as part of innate immunity. Increased resistance to extraintestinal Salmonella enteritidis (SE) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. Invasion of chicken epithelial cells by SE induces an 8- to 10-fold increase in interleukin (IL) -6 production. Infection with SE induces an influx of heterophils to the site of infection; therefore, we hypothesize heterophils would be responsive to IL-6. The objective was to determine the effects of COS cell-derived recombinant chicken interleukin 6 (rChIL-6) on in vitro functional activity of heterophils. Heterophils were incubated with rChIL-6 or mock-transfected COS cell supernatant and functional activity was assessed. Heterophils treated with rChIL-6 showed no functional differences compared to controls. These data indicate rChIL-6, alone, does not affect the functional activity of neonatal chicken heterophils in vitro. Therefore, the function of IL-6 in the local environment in response to SE invasion is still unknown.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Service, College Station, TX 77845, USA.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Ferro
authors.forename: Christina L
authors.initials: CL
authors.lastname: Swaggerty
authors.forename: Haiqi
authors.initials: H
authors.lastname: He
authors.forename: Lisa
authors.initials: L
authors.lastname: Rothwell
authors.forename: Pete
authors.initials: P
authors.lastname: Kaiser
authors.forename: Michael H
authors.initials: MH
authors.lastname: Kogut
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D002645:Chickens / Q000378:metabolism* / Q000382:microbiology; D006098:Granulocytes / Q000187:drug effects*; D015850:Interleukin-6 / Q000494:pharmacology*; D016897:Respiratory Burst / Q000502:physiology; D012480:Salmonella Infections / Q000378:metabolism; D012477:Salmonella enteritidis
chemical_list: D015850:Interleukin-6