title: [The immunological effects in guinea pig sensitized by trichloroethylene].
abstract: OBJECTIVE
  To investigate the changes of certain immunological indexes in development of Guinea Pig allergic dermatitis induced by trichloroethylene (TCE).
  
  
  METHODS
  The Guinea Pig model of TCE-induced allergic dermatitis was established by Guinea Pig Maximisation Test. IgG levels in serum were measured by sandwich enzyme immunoassay. The splenic T lymphocytes ConA-stimulated proliferation function and natural killer (NK) cell activity were detected by MTT assay and micro-LDH release assay, respectively.
  
  
  RESULTS
  The sensitization rate of TCE was 66.7%. The IgG level in serum of TCE-sensitized animals was significantly higher than that in normal animals (P < 0.05). No obvious differences in Splenic T lymphocytes proliferation index and NK cell activity between TCE-sensitized and normal animals were found (P > 0.05).
  
  
  CONCLUSIONS
  Humoral immunity may play an important role in development of allergic dermatitis induced by TCE.
authors.affiliation: National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. yf_dai@sina.com
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Yong
authors.initials: Y
authors.lastname: Nui
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.forename: Shug-Uang
authors.initials: SU
authors.lastname: Leng
mesh_terms: D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood*; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D013601:T-Lymphocytes / Q000276:immunology; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D007074:Immunoglobulin G; D014241:Trichloroethylene