Cell proliferation activity of proliferating bile duct after bile duct ligation in rats.
The cell proliferation activity of proliferating bile ducts produced by bile duct ligation (BDL) in rats was examined histologically, immunohistochemically, and ultrastructurally. Proliferating bile ducts, which were similar to normal bile ducts, increased with time after BDL. The cell proliferation activity of proliferating bile ducts, measured using proliferating-cell nuclear antigen and 5-bromo-2'-deoxyuridine antibodies, tended to be high at 1 and 3 days after BDL and decreased progressively at 2 to 4 weeks after BDL. On the other hand, alpha-smooth muscle actin-positive myofibroblast-like cells increased continuously after BDL. These findings indicate that there is a negative correlation between the cell proliferation activity of proliferating bile ducts and that of myofibroblast-like cells.
Yoshioka
K
K
Department of Veterinary Anatomy, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada Aomori 034, Japan. yoshioka@vmas.kitasato-u.ac.jp
Mori
A
A
Taniguchi
K
K
Mutoh
K
K
D000818:Animals; D001652:Bile Ducts / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure*; D049109:Cell Proliferation; D006652:Histological Techniques; D007150:Immunohistochemistry; D008026:Ligation; D008099:Liver / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D018809:Proliferating Cell Nuclear Antigen