title: Expression of nuclear receptor-transcription factor genes during Aedes aegypti midgut metamorphosis and the effect of methoprene on expression.
abstract: Exposure of mosquito 4th instars to the juvenile hormone analogue methoprene prevents the emergence of adults by interfering with metamorphosis. One metamorphic processes that is disrupted is midgut remodeling. To investigate the molecular mechanisms by which this occurs, the pattern of transcription factor gene expression during the Aedes aegypti (L.) 4th instar was investigated by the method of real time PCR. The results indicate that in untreated larvae, expression of transcription factors genes AHR3 and AaE75B increases within 24h after the last larval-larval molt, transcription of AaEcR-B, AaUSP-a and AassFTZ-F1 increases approximately 24h later, and transcription of AaE75A increases just before the larval-pupal molt. There is uniform expression of AaUSP-b throughout the 4th instar. The effect of methoprene exposure on transcription factor gene expression during midgut remodeling was investigated. The results indicate that, in a dose and stage dependent manner, methoprene affects increases in expression that normally occur during midgut remodeling. The coincident effects of methoprene on metamorphic midgut remodeling and on transcription factor gene expression suggests that the two processes are related.
authors.affiliation: Biology Department, Brooklyn College, City University of New York, 2900 Bedford Ave., Brooklyn, NY 11210, USA. jtnbc@cunyvm.cuny.edu
authors.forename: James T
authors.initials: JT
authors.lastname: Nishiura
authors.forename: Kathryn
authors.initials: K
authors.lastname: Ray
authors.forename: Jasen
authors.initials: J
authors.lastname: Murray
mesh_terms: D000330:Aedes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D041981:Gastrointestinal Tract / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000187:drug effects / Q000502:physiology*; D008726:Methoprene / Q000494:pharmacology*; D018978:Molting; D011679:Pupa / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D008726:Methoprene