Studies of a vascular-acting photosensitizer, Pd-bacteriopheophorbide (Tookad), in normal canine prostate and spontaneous canine prostate cancer.
OBJECTIVE
Photodynamic therapy (PDT) mediated with Tookad (Pd-bacteriopheophorbide, WST09) was investigated pre-clinically as part of a program to develop an alternative modality for treating prostate cancer.


METHODS
Spontaneous canine prostate cancer and normal canine prostate were used as the animal models. Interstitial PDT was performed by IV infusion of the photosensitizer and irradiating the prostates with a diode laser (763 nm). The prostates were harvested 1-week post-PDT and subjected to histopathologic examinations. The effects of the drug doses and light doses were studied for one- and two-session PDT. Pharmacokinetics were studied using HPLC assay. The feasibility of using perfusing CT scans for assessing PDT lesions was also evaluated.


RESULTS
Tookad is a vascular-acting drug and clears rapidly from the circulation. Tookad-PDT-induced lesions, in both normal and cancerous prostates, were characterized by marked hemorrhagic necrosis.


CONCLUSIONS
Tookad-PDT is very effective in ablating prostatic tissue through its vascular effects.
Huang
Zheng
Z
HealthONE Alliance, Denver, Colorado 80203, USA. zheng_huang@msn.com
Chen
Qun
Q
Luck
David
D
Beckers
Jill
J
Wilson
Brian C
BC
Trncic
Nadira
N
Larue
Susan M
SM
Blanc
Dominique
D
Hetzel
Fred W
FW
D000818:Animals; D001429:Bacteriochlorophylls / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D007262:Infusions, Intravenous; D008297:Male; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011467:Prostate / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
D001429:Bacteriochlorophylls; D017319:Photosensitizing Agents; C456120:palladium-bacteriopheophorbide