title: Macrolide-affected Toll-like receptor 4 expression from Helicobacter pylori-infected monocytes does not modify interleukin-8 production.
abstract: Macrolide antibiotics have an anti-inflammatory effect by suppressing lipopolysaccharide-induced IL-8 production. IL-8 secretion from monocytes is observed in Helicobacter pylori infection. Although cag gene products are known to induce IL-8 secretion, whether other bacterial substances can initiate the reaction is not determined. In this study, we show that clarithromycin induced down-regulation of Toll-like receptor 4 expression and did not lead to a decrease in IL-8 production and H. pylori lipopolysaccharide. However, Toll-like receptor 4 activation was possibly not the main cause in the induction of inflammation during H. pylori infection.
authors.affiliation: Department of Internal Medicine, Kangdong Sacred Heart Hospital, Hallym University, 445 Gil dong Gangdong-gu, Seoul 134-701, Republic of Korea.
authors.forename: Joon Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Hak Yang
authors.initials: HY
authors.lastname: Kim
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Myung Kook
authors.initials: MK
authors.lastname: Jang
authors.forename: Jin Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Yoo
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Han
authors.forename: Joon Soo
authors.initials: JS
authors.lastname: Hahm
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D008070:Lipopolysaccharides; D018942:Macrolides; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D017291:Clarithromycin