[Content analysis of bilirubin in niuhuang compound suppository by HPLC].
OBJECTIVE
To establish a method of determinating content of bilirubin in Niuhuang Compound Suppository by HPLC.


METHODS
C18 column was used. The mobile phase was consisted of dimethylsulfoxide: acetonitrile: 0.5% acetic ammonium (1:1.4:1). Detector wavelength was 456 nm. The extraction solution for bilirubin was consisted of dlimethylsulfoxide: acetonitrile = 9:4.


RESULTS
The linearity was obtained over the range of 0.107 - 2.14 microg. The mean recovery was 101.0%, RSD = 0.518% (n = 5).


CONCLUSIONS
The method was sensitive, simple and accurate.
Chen
Jing
J
Hubei College of Traditional Chinese Medicine, Wuhan 430061.
Gan
Guoping
G
He
Zai'an
Z
Liu
Yanwen
Y
Shi
Keli
K
D000818:Animals; D001663:Bilirubin / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D008419:Materia Medica / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013488:Suppositories; D013678:Technology, Pharmaceutical / Q000379:methods
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D013488:Suppositories; D001663:Bilirubin