[Detection of the metabolites of dehydroepiandrosterone in urine with gas chromatography-combustion-isotope ratio mass spectrometry].
OBJECTIVE
To establish a method to determine the isotope ratios of 13C to 12C of dehydroepiandrosterone and its metabolites in urine, for detecting the source of dehydroepiandrosterone or its metabolites.


METHODS
Preliminary separation of endogenous anabolic androgenic steroids could be achieved using solid phase extraction, enzymolysis and thin layer chromatography. The source of dehydroepiandrosterone and other endogenous anabolic androgenic steroids could be detected by their delta values with gas chromat ography-combustion-isotope ratio mass spectrometry.


RESULTS
The 5 values of some metabolites of dehydroepiandrosterone reduced after the administration of dehydroepiandrosterone preparation. In these cases the data indicated that exogenous anabolic androgenic steroids were administrated.


CONCLUSIONS
The source of dehydroepiandrosterone or its metabolites in urine could be detected by measuring their delta values with this method.
Wang
Jing-zhu
JZ
National Research Institute of Sports Medicine, Beijing 100029, China. jzhw@yahoo.com
Wu
Mou-tian
MT
Zhang
Yi-nong
YN
Liu
Xin
X
Yang
Zhi-yong
ZY
D000328:Adult; D015113:Androstane-3,17-diol / Q000652:urine; D000738:Androsterone / Q000652:urine*; D002855:Chromatography, Thin Layer / Q000379:methods; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D004300:Doping in Sports; D005043:Etiocholanolone / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D011279:Pregnanetriol / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods
D015113:Androstane-3,17-diol; D011279:Pregnanetriol; D003687:Dehydroepiandrosterone; D005043:Etiocholanolone; D000738:Androsterone