title: [Effect of fluoride on activities of enzyme and ultrastructure in primary cultured rat hepatocytes].
abstract: OBJECTIVE
  To study the cell viability, activities of enzyme and ultrastructure changes induced by sodium fluoride in primary cultured rat hepatocytes.
  
  
  METHODS
  Hepatocytes were isolated using half-in situ collagenase digestion method. Cellular viability was determined by MTT method. The activities of ALT and AST were determined by spectrophotography method. The ultrastructure changes of hepatocyte were observed under transmission electron microscope.
  
  
  RESULTS
  After cultured with various concentrations of fluoride for 24 hours, a dose-dependent decrease of cell viability was detected in the hepatocytes. The activities of AST and ALT in the 2 mmol/L and 4 mmol/L groups were significantly higher than the control group (P < 0.05). Transmission electron microscope study showed that in fluoride treated hepatocytes the changes included swollen mitochondria and disordered, disrupted endoplasm reticulum.
  
  
  CONCLUSIONS
  Excessive fluoride induced significant toxicity in primary cultured hepatocytes which manifested the injuries of membrane and organell plasma membrane.
authors.affiliation: School of Public Health, China Medical University, Shenyang 110001, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Guo
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Sun
authors.forename: Gui-Fan
authors.initials: GF
authors.lastname: Sun
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*
chemical_list: D012969:Sodium Fluoride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase