title: Preparation of novel mercury-doped silver nanoparticles film glassy carbon electrode and its application for electrochemical biosensor.
abstract: A novel mercury-doped silver nanoparticles film glassy carbon (Ag/MFGC) electrode was prepared in this study. Electrochemical behaviors of cysteine on the Ag/MFGC electrode were investigated by electrochemical impedance spectroscopy and cyclic voltammetry (CV). The results indicated that cysteine could be strongly adsorbed on the surface of the Ag/MFGC electrode to form a thin layer. The doped electrode could catalyze the electrode reaction process of cysteine, and the cysteine displayed a pair of well-defined and nearly reversible CV peaks at the electrode in an acetate buffer solution (pH 5.0). The Ag/MFGC electrode was used for determination of cysteine by differential pulse voltammetry. The linear range was between 4.0x10(-7) and 1.3x10(-5) mol/L, with a detection limit of 1.0x10(-7) mol/L and a signal-to-noise ratio of 3. The relative standard deviation was 2.4% for seven successive determinations of 1.0x10(-5) mol/L cysteine. The determinations of cysteine in synthetic samples and urinal samples were carried out and satisfactory results were obtained. Amperometric application of the Ag/MFGC electrode as biosensors is proposed.
authors.affiliation: School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China.
authors.forename: Mao-Guo
authors.initials: MG
authors.lastname: Li
authors.forename: Yong-Jia
authors.initials: YJ
authors.lastname: Shang
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Gao
authors.forename: Guang-Feng
authors.initials: GF
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Fang
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002244:Carbon; D003545:Cysteine / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D008628:Mercury; D049329:Nanostructures; D012834:Silver
chemical_list: D012834:Silver; D002244:Carbon; D008628:Mercury; D003545:Cysteine