[Influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice].
OBJECTIVE
To investigate the influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice.


METHODS
C57BL/6J mice, exposed to hypoxia in the man-made auto pressure and hypoxia control cabin (XQ-I), were divided into 3 groups: the normal control group, 24 h hypoxia group and 48 h hypoxia group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of leptin and leptin receptor.


RESULTS
(1) Compared with the normal control group (0.508 +/- 0.207), the leptin mRNA level of 24 h hypoxia group (0.903 +/- 0.190) and 48 h hypoxia group (0.856 +/- 0.336) were up-regulated markedly (all < 0.05); (2) Compared with the normal control group (Ra 0.630, Rb 0.258 +/- 0.049, Rc 0.133), the Ra, Rb, Rc mRNA levels of 24 h hypoxia group (Ra 0.724, Rb 0.381 +/- 0.038, Rc 0.299) and 48 h hypoxia group (Ra 0.700, Rb 0.345 +/- 0.042, Rc 0.292) were up-regulated to different degrees.


CONCLUSIONS
As an independent factor, hypoxia can stimulate increased expression of leptin, leptin receptor Ra, Rb, Rc mRNA. It is suggested that leptin play an important role in positive regulation of respiratory function.
Liu
Xi
X
Department of Gerontology, Third People's Hospital, Chongqing 400014, China.
Wu
Ya-mei
YM
Xu
Ling
L
Tang
Chao
C
Zhong
Yin-bao
YB
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000860:Hypoxia; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D054411:Receptors, Leptin; D013997:Time Factors
D020738:Leptin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse