Involvement of Bax, Bcl-2 and caspase 3 in hydroxyurea- or etoposide-induced apoptosis of mouse interleukin-3-dependent lymphoma cells.
BACKGROUND
The apoptosis action induced by hydroxyurea or etoposide in interleukin 3-dependent lymphoma cells (DA-1) was studied.


METHODS
The conditions to study apoptosis of these cells were 17 hours of cell treatment with concentrations of 1.25 mM hydroxyurea or 100 microM etoposide using flow cytometry, fluorometry and immunoblots techniques.


RESULTS
Time-dependent reductions of cell viability after these treatments were observed. Caspase 3 activity was highly activated in both cases. Chemical treatments or interleukin 3 withdrawal rendered level changes in Bax and Bcl-2 expression.


CONCLUSIONS
These results support the implication of these factors in DA-1 cell apoptosis induced by these chemical treatments or interleukin 3 depletion.
Olmos
Gemma
G
Departamento de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Prieto
Alfredo
A
Herráez
Angel
A
Tejedor
M Cristina
MC
Alvarez-Mon
Melchor
M
Diez
José C
JC
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D003470:Culture Media; D004790:Enzyme Induction / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; C494827:Bax protein, mouse; D003470:Culture Media; D007377:Interleukin-3; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D005047:Etoposide; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D006918:Hydroxyurea