Determination of diethanolamine in shampoo products containing fatty acid diethanolamides by liquid chromatography with a thermal energy analyzer.
A liquid chromatography (LC) method using a thermal energy analyzer (TEA) is described for the determination of diethanolamine (DEA) in shampoo products containing fatty acid diethanolamides. DEA was converted to N-nitrosodiethanolamine (NDELA) by dissolving a portion of the product in 6M acetic acid and mixing with sodium nitrite for 1 h at room temperature. The reaction mixture was dried, dissolved in acetone, and analyzed for NDELA by LC-TEA. The recovery of DEA from 2 shampoo products at fortification levels of 25, 250, and 1000 ppm ranged from 70 to 105%. Twenty shampoo products were analyzed by this method, and 19 were found to contain DEA at levels ranging from 140 to 15 200 ppm.
Chou
Hardy J
HJ
U.S. Food and Drug Administration, 5100 Paint Branch Pkwy, College Park, MD 20740, USA. hchou@cfsan.fda.gov
D000465:Algorithms; D002853:Chromatography, Liquid; D004983:Ethanolamines / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009603:Nitroso Compounds; D012915:Soaps / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D004983:Ethanolamines; D005227:Fatty Acids; D007202:Indicators and Reagents; D009603:Nitroso Compounds; D012915:Soaps