[Effect of Shuangshen Tongguan Recipe on nuclear factor-kappa B signal pathway and myocardial junction-mediated intercellular communication in acute myocardial ischemia/reperfusion injured model rats].
OBJECTIVE
To investigate the effects of Shuangshen Tongguan Recipe (SSTG) on myocardial nuclear factor-kappa B (NF-kappaB) signal pathway, expression of myocardial junction intercellular communication (MJIC) connexin 43 (Cx43), and infarcted myocardial size and weight of the rats' heart after acute myocardial ischemia/reperfusion (I/R) damage.


METHODS
Model rat of I/R injury was established by coronary arterial ligating/ releasing. The infarcted myocardial size and weight were determined by N-BT staining, expression of NF-kappaB p65 in myocardial tissue and Cx43 were determined by immunohistochemical method, contents of serum tumor necrosis factor-alpha(TNF-alpha) and intercellular adhesion molecule-1 (ICAM-1) were measured by ABC-ELISA.


RESULTS
The myocardial infarcted size and weight, expression of NF-kappaB p65, contents of serum TNF-alpha and ICAM-1 of I/R injured rats in the model group were significantly increased (P<0.05), while Cx43 degraded markedly after modeling. These changes were restored after treated with SSTG (P <0.05).


CONCLUSIONS
Serious myocardial infarction occurs after ischemia/reperfusion injury, combined with NF-kappaB signal pathway activation and severe Cx43 degradation. SSTG could inhibit the activation of NF-kappaB, the over-excretion of TNF-alpha and ICAM-1 in serum, and the degradation of Cx43 to decrease the myocardial infarcted size and weight.
Liu
Jian-xun
JX
Experiment Research Center, Xiyuan Hospital, China Academy of TCM, Beijing. jianxun@ht.rol.cn.net
Han
Xiao
X
Ma
Xiao-bin
XB
D000818:Animals; D018031:Connexin 43 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000188:drug therapy; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis / Q000502:physiology*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
D018031:Connexin 43; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1