Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human platelet antigen as a model.
BACKGROUND
Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins.


METHODS
By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems.


RESULTS
Unambiguous and concordant results were obtained with both techniques for all samples.


CONCLUSIONS
The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
Bugert
Peter
P
Institute of Transfusion Medicine and Immunology, University of Heidelberg, Faculty of Clinical Medicine, Mannheim, Germany.
McBride
Simon
S
Smith
Graham
G
Dugrillon
Alex
A
Klüter
Harald
H
Ouwehand
Willem H
WH
Metcalfe
Paul
P
D016824:Antigens, Human Platelet / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005838:Genotype; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007519:Isoantigens / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D020641:Polymorphism, Single Nucleotide; D019914:Taq Polymerase
D016824:Antigens, Human Platelet; D007519:Isoantigens; D015345:Oligonucleotide Probes; D019914:Taq Polymerase