A selective and rapid method for the quantification of captopril in human plasma using liquid chromatography/selected reaction monitoring mass spectrometry.
A specific hyphenated high performance liquid chromatography-mass spectrometric (LC-MS/MS) assay was developed for the determination of captopril in plasma. The drug was extracted from plasma using liquid-liquid extraction with a mixture of diethylether:dichloromethane. After the addition of the internal standard, samples were applied to a prepacked C8 Waters Symmetry column. The ion trap MS/MS detector was equipped with electrospray ionization (ESI) source operating in the positive ion mode. Drug determination was accomplished monitoring captopril at molecular ion m/z 218 and MS/MS (daughter) at m/z 171.6. The method was applied to captopril determination in human plasma after the administration of captopril 50 mg tablets to healthy volunteers who have participated in a pharmacokinetic study. The method was proved to be specific and precise by testing six different plasma batches. Linearity was established for the range of concentrations 25-3000 ng/ml with a regression factor of 0.9995. Intra-day accuracy ranged from 90.16 to 96.18%, while the intra-day precision ranged from 2.60 to 9.66% at the concentrations of 75, 1440 and 2500 ng/ml. Inter-day precision of the method ranged from 5.04 to 10.10%. This validated method of analysis was successfully applied to human plasma analyses after the administration of a single dose of 50 mg captopril tablets to healthy volunteers.
Salem
Isam Ismail
II
ACDIMA Center for Bioequivalence and Pharmaceutical Studies, P.O. Box 925161, Amman 11190, Jordan. dr.salem@acdima.com
Saif
Weasm Abu
WA
Jmeian
Yazen
Y
Al Tamimi
Jaafar I
JI
D002216:Captopril / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors
D002216:Captopril