Allosteric aptamers and aptazymes as probes for screening approaches.
Substantial effort is currently being devoted to engineering allosteric nucleic acids, aptamers and ribozymes for various applications in cellular and molecular biology, biotechnology and diagnostics. These molecular switches alter their different functional activities in response to specific binding molecules, including proteins, nucleic acids and small organic compounds. The interacting molecules trigger a response in the allosteric nucleic acid, which can be used for purposes such as real-time monitoring, high-throughput screening or gene expression control.
Famulok
Michael
M
Rheinische Friedrich-Wilhelms-Universität Bonn, Kekulé Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Strasse 1, Bonn 53121, Germany. m.famulok@uni-bonn.de
D000494:Allosteric Regulation / Q000235:genetics / Q000502:physiology; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008024:Ligands; D015335:Molecular Probes; D011506:Proteins / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism
D008024:Ligands; D015335:Molecular Probes; D011506:Proteins; D016337:RNA, Catalytic