Detection of p16 hypermethylation in gastric carcinomas using a seminested methylation-specific PCR.
Aberrant DNA methylation of a CpG site is among the earliest and most frequent alterations in various tumors including gastric carcinoma. The aim of this study is to detect tumor-associated aberrant hypermethylation of the p16 gene from 60 gastric tumor and corresponding normal tissues using a seminested methylation-specific PCR (MSP). The results indicated that hypermethylation of the p16 gene could be detected in 80% (48/60) of the gastric tumor samples from the first PCR. However, the frequency increased significantly to 86.7% (52/60) of the gastric tumor samples after the second PCR. These results show that this technique increases the sensitivity of detecting p16 hypermethylation from tumor samples. Furthermore, the aberrant methylation of p16 was observed in all of the stages, confirming that this epigenetic alteration is an early event during gastric carcinogenesis. Clinicopathologic parameters such as age, sex, and histological differentiation of GC were not significantly associated with the methylation status.
Hou
Peng
P
Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
Ji
Mei-Ju
MJ
Shen
Jia-Yao
JY
He
Nong-Yue
NY
Lu
Zu-Hong
ZH
D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000737:chemistry; D005260:Female; D019942:Genes, p16; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics*; D013447:Sulfites / Q000737:chemistry
D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; D013447:Sulfites; C009279:sodium bisulfite