title: A method for screening for various sedative-hypnotics in serum by liquid chromatography/single quadrupole mass spectrometry.
abstract: A screening method for the detection of sedative-hypnotics in serum is described. The target drugs, which include practically all the sedative-hypnotics distributed in Japan, consisted of 5 barbiturates, 30 benzodiazepine-related drugs and 11 other sedative-hypnotics (i.e., apronalide, bromisovalum, chloral hydrate, triclofos, chlorpromazine, promethazine, diphenhydramine, hydroxyzine, zopiclone, zolpidem and tandospirone). Thirty-nine analytes, selected in terms of the pharmacokinetics of the target drugs, in human serum were screened using a combination of mixed-mode solid-phase extraction and liquid chromatography/electrospray-ionization single-quadrupole mass spectrometry. The detection limits (non-basic analytes, 1-50 ng/ml; basic analytes, 0.1-5 ng/ml) were sufficient to permit the screening of a single therapeutic administration of a target drug.
authors.affiliation: National Research Institute of Police Science, Kashiwa-shi, Chiba, Japan. miyaguchi@nrips.go.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Miyaguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yuko T
authors.initials: YT
authors.lastname: Iwata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Tohru
authors.initials: T
authors.lastname: Kishi
mesh_terms: D005260:Female; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006993:Hypnotics and Sedatives