The expression of integrin alpha(v)beta6 promotes the epithelial cell morphology and suppresses invasive behavior in transformed oral keratinocytes.
The expression of the integrin alpha(v)beta6 has been correlated with oral SCC invasion. We evaluated its expression in three 4NQO transformed murine oral keratinocyte cell lines (B7E3, B7E11 and B4B8). The B7E3 cells were negative for beta6, whereas the B7E11 and the B4B8 cells were both positive. The beta6 negative B7E3 cells were fibroblast-like in appearance, whereas the B7E11 cells were more epithelial-like. The B4B8 cells were a mixture of the two cell types. Using immunofluorescent microscopy, we found that vimentin was highly expressed in the B7E3 cells, whereas the B7E11 cells keratin positive. The B4B8 cells expressed both filaments. The B7E3 cells formed large tumors when injected into nude mice, whereas the B4B8 cells formed small tumors and the B7E11 cells formed none. These results suggest that the expression of the alpha(v)beta6 integrin suppresses tumor formation and may promote the epithelial phenotype in 4NQO-transformed murine oral keratinocytes.
Mogi
Seiki
S
Department of Orofacial Sciences, 513 Parnassus Ave, C415 Box 0422 University of California, San Francisco, San Francisco, CA 94143-2922, USA.
Dang
Dongmin
D
Van Waes
Carter
C
Ellis
Duncan
D
Atakilit
Amha
A
Ramos
Daniel M
DM
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000502:physiology*; D048708:Cell Growth Processes / Q000502:physiology; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins; D005434:Flow Cytometry; D039641:Integrin beta Chains / Q000096:biosynthesis; D019012:Integrin beta1 / Q000096:biosynthesis; D016023:Integrins / Q000096:biosynthesis / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009055:Mouth / Q000166:cytology; D014746:Vimentin / Q000096:biosynthesis
D000951:Antigens, Neoplasm; D005353:Fibronectins; D039641:Integrin beta Chains; D019012:Integrin beta1; D016023:Integrins; D014746:Vimentin; C073878:integrin alphavbeta6; C072170:integrin beta6