title: The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation.
abstract: Amino acid-starved yeast activates the eIF2alpha kinase Gcn2p to suppress general translation and to selectively derepress the transcription factor Gcn4p, which induces various biosynthetic genes to elicit general amino acid control (GAAC). Well-fed yeast activates the target of rapamycin (TOR) to stimulate translation via the eIF4F complex. A crosstalk was demonstrated between the pathways for GAAC and TOR signaling: the TOR-specific inhibitor rapamycin activates Gcn2p. Here we demonstrate that, upon TOR-inactivation, the putative TOR-regulated eIF4E-associated protein Eap1p likely functions downstream of Gcn2p to attenuate GCN4 translation via a mechanism independent of eIF4E-binding, thereby constituting another interface between the two pathways.
authors.affiliation: Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Matsuo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kubota
authors.forename: Tohru
authors.initials: T
authors.lastname: Obata
authors.forename: Keiji
authors.initials: K
authors.lastname: Kito
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ota
authors.forename: Takanari
authors.initials: T
authors.lastname: Kitazono
authors.forename: Setsuro
authors.initials: S
authors.lastname: Ibayashi
authors.forename: Takuma
authors.initials: T
authors.lastname: Sasaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Iida
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D020123:Sirolimus / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C410795:eIF4E-associated protein Eap1; D011494:Protein Kinases; C512912:GCN2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C500749:target of rapamycin protein, S cerevisiae; D020123:Sirolimus