title: Heterodimer formation between c-Jun and Jun B proteins mediated by Epstein Barr virus encoded latent membrane protein 1.
abstract: Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) may trigger the transcription factor AP-1 including c-Jun and c-fos. In this report, using a Tet-on LMP1 HNE2 cell line which is a dual-stable LMP1 integrated nasopharyngeal carcinoma (NPC) cell line and the expression of LMP1 in which could be regulated by the Tet-on system, we show that Jun B can efficiently form a new heterodimeric complex with the c-Jun protein under the regulation of LMP1, phosphorylation of c-Jun (ser 63, ser 73) and Jun B is involved in the process of the new heterodimeric formation. We also find that this heterodimeric form can bind to the AP-1 consensus sequence. Transfection studies suggest that JNK interaction protein (JIP) could inhibit the heterodimer formation of c-Jun and Jun B through blocking the AP-1 signaling pathway triggered by LMP1. The interaction and function between c-Jun protein and Jun B protein increase the repertoire of possible regulatory complexes by LMP1 that could play an important role in the regulation of transcription of specific cellular genes in the process of genesis of nasopharyngeal carcinoma.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South of University, Changsha 410078, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Tao
authors.forename: Yunnian
authors.initials: Y
authors.lastname: Tan
authors.forename: Leo M
authors.initials: LM
authors.lastname: Lee
authors.forename: Xiyun
authors.initials: X
authors.lastname: Deng
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wu
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D004586:Electrophoresis; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry*; D015398:Signal Transduction; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000737:chemistry*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D014763:Viral Matrix Proteins; D004247:DNA