title: Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae.
abstract: Protein kinase C is apparently involved in the control of many cellular systems: the cell wall integrity pathway, the synthesis of ribosomes, the appropriated reallocation of transcription factors under specific stress conditions and also the regulation of N-glycosylation activity. All these observations suggest the existence of additional targets not yet identified. In the context of the control of carbon metabolism, previous data had demonstrated that Pkc1p might play a central role in the control of cellular growth and metabolism in yeast. In particular, it has been suggested that it might be involved in the derepression of genes under glucose-repression by driving an appropriated subcellular localization of transcriptional factors, such as Mig1p. In this work, we show that a pkc1Delta mutant is unable to grow on glycerol because it cannot perform the derepression of the GUT1 gene that encodes glycerol kinase. Additionally, active transport is also partially affected. Using this phenotype, we were able to isolate a new pkc1Delta revertant. We also isolated two transformants identified as the nuclear exportin Msn5 and the histone deacetylase Hos2 extragenic suppressors of this mutation. Based on these results, we postulate that Pkc1p may be involved in the control of the cellular localization and/or regulation of the activity of nuclear proteins implicated in gene expression.
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Deparatmento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, 35.400-000 Ouro Preto, MG, Brazil.
authors.forename: Katia N
authors.initials: KN
authors.lastname: Gomes
authors.forename: Suzy M A C
authors.initials: SM
authors.lastname: Freitas
authors.forename: Thiago M
authors.initials: TM
authors.lastname: Pais
authors.forename: Juliana L R
authors.initials: JL
authors.lastname: Fietto
authors.forename: António H
authors.initials: AH
authors.lastname: Totola
authors.forename: Rosa M E
authors.initials: RM
authors.lastname: Arantes
authors.forename: António
authors.initials: A
authors.lastname: Martins
authors.forename: Cândida
authors.initials: C
authors.lastname: Lucas
authors.forename: Dorit
authors.initials: D
authors.lastname: Schuller
authors.forename: Margarida
authors.initials: M
authors.lastname: Casal
authors.forename: Ieso M
authors.initials: IM
authors.lastname: Castro
authors.forename: Luciano G
authors.initials: LG
authors.lastname: Fietto
authors.forename: Rogelio L
authors.initials: RL
authors.lastname: Brandão
mesh_terms: D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000235:genetics; D011493:Protein Kinase C / Q000172:deficiency* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D005991:Glycerol Kinase; C490702:PKC1 protein, S cerevisiae; D011493:Protein Kinase C; D005990:Glycerol