title: Insertion sequence profiling of UK Mycoplasma bovis field isolates.
abstract: The presence and distribution of insertion sequences ISMbov2 and ISMbov3 within Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221 bp and 185 bp, to detect ISMbov2 and ISMbov3, respectively, amplified from the homologous sequences ISMmy1 and IS1634 within Mycoplasma mycoides subspecies mycoides small colony type. We present data obtained from 49 field isolates of M. bovis, originating from pneumonic lungs, collected within the United Kingdom between 1996 and 2002. Hybridisation profiles show considerable variation between strains. ISMbov2 sequences are present between 2 and 17 copies while there are between 3 and 14 copies of the IS1634 homologue ISMbov3. These data also provide support for previous analysis by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
authors.affiliation: Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. k.miles@vla.defra.gsi.gov.uk
authors.forename: Katie
authors.initials: K
authors.lastname: Miles
authors.forename: Laura
authors.initials: L
authors.lastname: McAuliffe
authors.forename: Anja
authors.initials: A
authors.lastname: Persson
authors.forename: Roger D
authors.initials: RD
authors.lastname: Ayling
authors.forename: Robin A J
authors.initials: RA
authors.lastname: Nicholas
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016000:Cluster Analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008171:Lung Diseases / Q000382:microbiology / Q000662:veterinary*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping / Q000662:veterinary; D006113:United Kingdom
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial