title: Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human platelet antigen as a model.
abstract: BACKGROUND
  Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins.
  
  
  METHODS
  By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems.
  
  
  RESULTS
  Unambiguous and concordant results were obtained with both techniques for all samples.
  
  
  CONCLUSIONS
  The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
authors.affiliation: Institute of Transfusion Medicine and Immunology, University of Heidelberg, Faculty of Clinical Medicine, Mannheim, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Bugert
authors.forename: Simon
authors.initials: S
authors.lastname: McBride
authors.forename: Graham
authors.initials: G
authors.lastname: Smith
authors.forename: Alex
authors.initials: A
authors.lastname: Dugrillon
authors.forename: Harald
authors.initials: H
authors.lastname: Klüter
authors.forename: Willem H
authors.initials: WH
authors.lastname: Ouwehand
authors.forename: Paul
authors.initials: P
authors.lastname: Metcalfe
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005838:Genotype; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007519:Isoantigens / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D020641:Polymorphism, Single Nucleotide; D019914:Taq Polymerase
chemical_list: D016824:Antigens, Human Platelet; D007519:Isoantigens; D015345:Oligonucleotide Probes; D019914:Taq Polymerase