title: Progesterone receptor subtypes in vascular smooth muscle cells of human aorta.
abstract: Progesterone is involved in various functions of the cardiovascular system, including those of vascular smooth muscle cells (VSMCs) via progesterone receptor (PR). Progesterone has also been postulated to be involved in inhibition of VSMC proliferation via PR. However, the details of PR expression have remained largely unknown in human cardiovascular VSMCs. Therefore, we first examined the relative levels of PR isoform (PR-A and PR-B) expression in VSMCs, using both immunohistochemistry and quantitative RT-PCR analysis. PR-B was equally expressed between male and female aorta, but PR-A was more abundant in female than in male aorta. This finding demonstrated that the status of PR subtype expression was associated with the difference of genders.
authors.affiliation: Department of Pathology, Tohoku University School of Medicine, Seiryomachi, Sendai, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Inoue
authors.forename: Chika
authors.initials: C
authors.lastname: Tazawa
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ono
authors.forename: Takuya
authors.initials: T
authors.lastname: Moriya
authors.forename: Haruo
authors.initials: H
authors.lastname: Saito
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Shoki
authors.initials: S
authors.lastname: Takahashi
authors.forename: Shogo
authors.initials: S
authors.lastname: Yamada
authors.forename: Hironobu
authors.initials: H
authors.lastname: Sasano
mesh_terms: D000328:Adult; D000368:Aged; D001012:Aorta, Abdominal / Q000737:chemistry*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D032389:Myocytes, Smooth Muscle / Q000737:chemistry*; D017698:Postmenopause; D017697:Premenopause; D012333:RNA, Messenger / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
chemical_list: D012333:RNA, Messenger; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B