title: Deletion of the yiaMNO transporter genes affects the growth characteristics of Escherichia coli K-12.
abstract: Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been functionally analysed. The yiaMNO genes of Escherichia coli K-12 encode one family member that transports the rare pentose l-xylulose. Its physiological role is unknown, since wild-type E. coli K-12 does not utilize l-xylulose as sole carbon source. Deletion of the yiaMNO genes in E. coli K-12 strain MC4100 resulted in remarkable changes in the transition from exponential growth to the stationary phase, high-salt survival and biofilm formation.
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Titia H
authors.initials: TH
authors.lastname: Plantinga
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Chris
authors.initials: C
authors.lastname: van der Does
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Danuta
authors.initials: D
authors.lastname: Tomkiewicz
authors.affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Geertje
authors.initials: G
authors.lastname: van Keulen
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Wil N
authors.initials: WN
authors.lastname: Konings
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750, AA Haren, The Netherlands.
authors.forename: Arnold J M
authors.initials: AJM
authors.lastname: Driessen
mesh_terms: D018441:Biofilms / Q000254:growth & development; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009876:Operon; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D014996:Xylulose / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D012965:Sodium Chloride; D014996:Xylulose