title: Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) was applied to isolate and purify bioactive compounds in the essential oil of the rhizomes of Curcuma wenyujin. Two sesquiterpenes germacrone and curdione were successfully isolated and purified using two-phase solvent system composed of petroleum ether-ethanol-diethyl ether-water (5/4/0.5/1, v/v) in tail to head elution mode. 62 mg of germacrone and 93 mg of curdione were obtained from 658 mg of the essential oil each at over 95% purity. Their structures were identified by 1H NMR and EI MS.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. yanjizhong@hzcnc.com
authors.forename: Jizhong
authors.initials: J
authors.lastname: Yan
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Tong
authors.forename: Yeping
authors.initials: Y
authors.lastname: Feng
authors.forename: Liuqing
authors.initials: L
authors.lastname: Sheng
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009822:Oils, Volatile / Q000737:chemistry*; D045788:Sesquiterpenes, Germacrane / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007202:Indicators and Reagents; D009822:Oils, Volatile; D045788:Sesquiterpenes, Germacrane; C504126:curdione; C048393:germacrone