title: [Application of SensiScript enzyme to a RT-PCR system for the obtention of RNA of HIV-1 starting serum samples stored at -20 degrees C during a decade].
abstract: The efficiency of SensiScript reverse transciptase to obtain useful genetic material in the sequencing of the nucleic acid from HIV-1, starting from sera collected between 1989 and 1998 and kept at suboptimal temperatures, was proved. On using the SensiScript enzyme it was obtained an amplification of the RNA of the HIV-1 in 86.5 % of the studied samples, compared with 20 % on using the AMV-RT enzyme . No amplification was obtained in 13.5 % of the studied samples with any of the 2 enzymes used.
authors.affiliation: Instituto de Medicina Tropical "Pedro Kouri" Ciudad de La Habana, Cuba. sresik@ipk.sld.cu
authors.forename: Sonia Resik
authors.initials: SR
authors.lastname: Aguirre
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Ping
authors.forename: Julio A
authors.initials: JA
authors.lastname: Barrios Olivera
authors.forename: Vivian Kourí
authors.initials: VK
authors.lastname: Cardellá
authors.forename: Ronald
authors.initials: R
authors.lastname: Swanstrom
mesh_terms: D001769:Blood / Q000821:virology; D015925:Cryopreservation; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D012367:RNA, Viral