title: Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography.
abstract: Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of quercetin-3-O-L-rhamnoside from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase-system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5 (v/v/v). In a single operation, 41.9mg of quercetin-3-O-L-rhamnoside was obtained from 366mg of the crude extract. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of quercetin-3-O-L-rhamnoside was over 96%. Its structure was identified by MS, 1H NMR and 13C NMR. Quercetin-3-O-L-rhamnoside was obtained from this plant for the first time.
authors.affiliation: Department of Biology, Graduate School of Chinese Academy of Sciences, Beijing 10049, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
authors.forename: Weixi
authors.initials: W
authors.lastname: Tian
authors.forename: Linhuan
authors.initials: L
authors.lastname: Wu
authors.forename: Xueli
authors.initials: X
authors.lastname: Cao
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D031002:Acer / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D011794:Quercetin / Q000302:isolation & purification*
chemical_list: D011794:Quercetin