title: Annexins I and IV inhibit Staphylococcus aureus attachment to human macrophages.
abstract: Annexins are a family of proteins that bind to phospholipids and carbohydrates in a calcium-dependent manner. They are present in a variety of body fluids. Previous studies have shown that annexins have anti-inflammatory activities for lipid A of Gram-negative bacteria. The present study investigated the effect of annexins on interaction between Gram-positive bacteria and immune cells such as macrophages. Annexins I and IV bound to lipoteichoic acids which are surface molecules on Gram-positive bacteria. Binding of annexins I and IV to whole Staphylococcus aureus (S. aureus) were observed and these bindings were inhibited by lipoteichoic acid from S. aureus. Moreover, annexins I and IV suppressed the attachment of S. aureus to phorbol 12-myristate 13-acetate-treated THP-1 cells (human macrophages). These results suggest that annexins I and IV have ligand specificities toward foreign substances, and that the annexins might have some anti-inflammatory property for Gram-positive bacteria.
authors.affiliation: Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Gotoh
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Takamoto
authors.forename: Kahori
authors.initials: K
authors.lastname: Kurosaka
authors.forename: Junko
authors.initials: J
authors.lastname: Masuda
authors.forename: Michiru
authors.initials: M
authors.lastname: Ida
authors.forename: Ayano
authors.initials: A
authors.lastname: Satoh
authors.forename: Eiji
authors.initials: E
authors.lastname: Takayama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kojima-Aikawa
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Matsumoto
mesh_terms: D017305:Annexin A1 / Q000378:metabolism / Q000494:pharmacology*; D017314:Annexin A4 / Q000378:metabolism / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000382:microbiology*; D010703:Phorbol Esters / Q000494:pharmacology; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology*; D013682:Teichoic Acids / Q000378:metabolism
chemical_list: D017305:Annexin A1; D017314:Annexin A4; D008070:Lipopolysaccharides; D010703:Phorbol Esters; D013682:Teichoic Acids; C009900:lipoteichoic acid; C011986:phorbol 12-tiglate 13-decanoate; D016650:Fluorescein-5-isothiocyanate