Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques.
Theresponse of Corynebacterium glutamicum to ammonium limitation was studied by transcriptional and proteome profiling of cells grown in a chemostat. Our results show that ammonium-limited growth of C. glutamicum results in a rearrangement of the cellular transport capacity, changes in metabolic pathways for nitrogen assimilation, amino acid biosynthesis, and carbon metabolism, as well as a decreased cell division. Since transcription at different growth rates was studied, it was possible to distinguish specific responses to ammonium limitation and more general, growth rate-dependent alterations in gene expression. The latter include a number of genes encoding ribosomal proteins and genes for F(o)F(1)-ATP synthase subunits.
Silberbach
Maike
M
Institut für Biochemie, Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany.
Schäfer
Mathias
M
Hüser
Andrea T
AT
Kalinowski
Jörn
J
Pühler
Alfred
A
Krämer
Reinhard
R
Burkovski
Andreas
A
D000222:Adaptation, Physiological; D000596:Amino Acids / Q000096:biosynthesis; D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D004734:Energy Metabolism; D009584:Nitrogen / Q000378:metabolism; D020543:Proteome; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D014158:Transcription, Genetic
D000596:Amino Acids; D020543:Proteome; D000644:Quaternary Ammonium Compounds; D002244:Carbon; D009584:Nitrogen