Use of microsatellites for parentage and kinship analyses in animals.
Microsatellite markers are quickly becoming the molecular marker of choice for studies of parentage and kinship in animals. In this chapter, we review methods and give protocols for screening potential microsatellite markers, as well as protocols for genotyping individuals with useful markers once they have been identified. In addition, we explain how microsatellites can be used to assess parentage and kinship, give basic analytical methods, and briefly review more sophisticated approaches that can be used to circumvent many of the problems that arise in any real empirical study.
Webster
Michael S
MS
School of Biological Sciences, Washington State University, Pullman, Washington 99164-4236, USA.
Reichart
Letitia
L
D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D003627:Data Interpretation, Statistical; D005260:Female; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005821:Genetic Techniques / Q000295:instrumentation / Q000706:statistics & numerical data; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D012984:Software; D020308:Songbirds / Q000235:genetics
D017931:DNA Primers; D005456:Fluorescent Dyes; D005819:Genetic Markers; D004247:DNA