Establishment and characterization of a new human glioblastoma cell line, NYGM.
A cell line designated NYGM was established from a human cerebral glioblastoma multiforme (GBM) obtained from a 75-year-old Japanese woman. The cell line has grown slowly without interruption and has been propagated continuously by serial passages (more than 80 passage) during the past 3 years. The cultured cells were fusiform or polyhedral in shape. The population doubling time was 24 hours. The chromosomal number varied between 77 and 88, with modal chromosomal number of 84. NYGM cells concomitantly expressed MET receptor tyrosine kinase (a product of c-met protooncogene) and its ligand HGF/SF (hepatocyte growth factor/scatter factor), as well as HGF activator and HGF activator inhibitors. The cells might be useful for the study of pericellular regulation of HGF/SF-MET signaling and HGF activation of GBM cells.
Nuki
Yoshitsugu
Y
Department of Neurosurgery, Faculty of Medicine, University of Miyazaki.
Uchinokura
Shunro
S
Miyata
Shiro
S
Fukushima
Tsuyoshi
T
Hamasuna
Ryouichi
R
Nakano
Shinichi
S
Wakisaka
Shinichiro
S
Akiyama
Yutaka
Y
Itoh
Hiroshi
H
Kataoka
Hiroaki
H
D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D000076202:c-Mer Tyrosine Kinase
D008024:Ligands; D011518:Proto-Oncogene Proteins; D017228:Hepatocyte Growth Factor; C097072:MERTK protein, human; D020794:Receptor Protein-Tyrosine Kinases; D000076202:c-Mer Tyrosine Kinase; C081092:HGF activator; D012697:Serine Endopeptidases