title: Characterization of renal interstitial fibroblast-specific protein 1/S100A4-positive cells in healthy and inflamed rodent kidneys.
abstract: Fibrosis is considered as a central factor in the loss of renal function in chronic kidney diseases. The origin of fibroblasts and myofibroblasts that accumulate in the interstitium of the diseased kidney is still a matter of debate. It has been shown that accumulation of myofibroblasts in inflamed and fibrotic kidneys is associated with upregulation of fibroblast-specific protein 1 (FSP1, S100A4), not only in the renal interstitium but also in the injured renal epithelia. The tubular expression of FSP1 has been taken as evidence of myofibroblast formation by epithelial-mesenchymal transition (EMT). The identity of FSP1/S100A4 cells has not been defined in detail. We originally intended to use FSP1/S100A4 as a marker of putative EMT in a model of distal tubular injury. However, since the immunoreactivity of FSP1 did not seem to fit with the distribution and shape of fibroblasts or myofibroblasts, we undertook the characterization of FSP1/S100A4-expressing cells in the interstitium of rodent kidneys. We performed immunolabeling for FSP1/S100A4 on thin cryostat sections of perfusion-fixed rat and mouse kidneys with peritubular inflammation, induced by thiazides and glomerulonephritis, respectively, in combination with ecto-5'-nucleotidase (5'NT), recognizing local cortical peritubular fibroblasts, with CD45, MHC class II, CD3, CD4 and Thy 1, recognizing mononuclear cells, with alpha smooth muscle actin (alphaSMA), as marker for myofibroblasts, and vimentin for intracellular intermediate filaments in cells of mesenchymal origin. In the healthy interstitium of rodents the rare FSP1/S100A4+ cells consistently co-expressed CD45 or lymphocyte surface molecules. Around the injured distal tubules of rats treated for 3-4 days with thiazides, FSP1+/S100A4+, 5'NT+, alphaSMA+, CD45+ and MHC class II+ cells accumulated. FSP1+/S100A4+ cells consistently co-expressed CD45. In the inflamed regions, alphaSMA was co-expressed by 5'NT+ cells. In glomerulonephritic mice, FSP1+/S100A4+ cells co-expressed Thy 1, CD4 or CD3. Thus, in the inflamed interstitium around distal tubules of rats and of glomerulonephritic mice, the majority of FSP1+ cells express markers of mononuclear cells. Consequently, the usefulness of FSP1/S100A4 as a tool for detection of (myo)fibroblasts in inflamed kidneys and of EMT in vivo is put into question. In the given rat model the consistent co-expression of alphaSMA and 5'NT suggests that myofibroblasts originate from resident peritubular fibroblasts.
authors.affiliation: Anatomical Institute, Division of Vegetative Anatomy, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Le Hir
authors.forename: Ivan
authors.initials: I
authors.lastname: Hegyi
authors.forename: Dominique
authors.initials: D
authors.lastname: Cueni-Loffing
authors.forename: Johannes
authors.initials: J
authors.lastname: Loffing
authors.forename: Brigitte
authors.initials: B
authors.lastname: Kaissling
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis*; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology; D005921:Glomerulonephritis / Q000378:metabolism / Q000473:pathology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D007668:Kidney / Q000737:chemistry* / Q000473:pathology; D007684:Kidney Tubules / Q000737:chemistry / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D051379:Mice; D009130:Muscle, Smooth / Q000737:chemistry; D051381:Rats; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000032:analysis*
chemical_list: D000199:Actins; D017252:CD3 Complex; D002135:Calcium-Binding Proteins; D000949:Histocompatibility Antigens Class II; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C470944:S100a4 protein, mouse; C499412:S100a4 protein, rat; D017493:Leukocyte Common Antigens