title: Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form.
abstract: Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively.
authors.affiliation: AFSSA Nancy, Laboratoire d'Etudes et de Recherches sur la Rage et la Pathologie des Animaux Sauvages, Domaine de Pixérécourt-B.P. 9, Malzéville F 54220, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Boucher
authors.forename: R
authors.initials: R
authors.lastname: Hanosset
authors.forename: D
authors.initials: D
authors.lastname: Augot
authors.forename: J M
authors.initials: JM
authors.lastname: Bart
authors.forename: M
authors.initials: M
authors.lastname: Morand
authors.forename: R
authors.initials: R
authors.lastname: Piarroux
authors.forename: F
authors.initials: F
authors.lastname: Pozet-Bouhier
authors.forename: B
authors.initials: B
authors.lastname: Losson
authors.forename: F
authors.initials: F
authors.lastname: Cliquet
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D048210:Echinococcus multilocularis / Q000302:isolation & purification*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005602:France; D006651:Histocytochemistry / Q000662:veterinary; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D034421:Sus scrofa / Q000469:parasitology*; D013553:Swine Diseases / Q000469:parasitology* / Q000473:pathology
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV