Human mitochondrial peroxiredoxin 5 protects from mitochondrial DNA damages induced by hydrogen peroxide.
Peroxiredoxin 5 is a thioredoxin peroxidase ubiquitously expressed in mammalian tissues. Peroxiredoxin 5 can be addressed intracellularly to mitochondria, peroxisomes, the cytosol and the nucleus. Here, we show that mitochondrial human peroxiredoxin 5 protects mitochondrial DNA (mtDNA) from oxidative attacks. In an acellular assay, recombinant peroxiredoxin 5 was shown to protect plasmid DNA from damages induced by metal-catalyzed generation of reactive oxygen species. In Chinese hamster ovary cells, overexpression of mitochondrial peroxiredoxin 5 significantly decreased mtDNA damages caused by exogenously added hydrogen peroxide. Altogether our results suggest that mitochondrial peroxiredoxin 5 may play an important role in mitochondrial genome stability.
Banmeyer
Ingrid
I
Laboratory of Cell Biology, Institut des Sciences de la Vie, Université catholique de Louvain, Belgium.
Marchand
Cécile
C
Clippe
André
A
Knoops
Bernard
B
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D054464:Peroxiredoxins; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012015:Reference Standards
D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D010544:Peroxidases; C515974:PRDX5 protein, human; D054464:Peroxiredoxins