Induction of DNA repair proteins, Ref-1 and XRCC1, in adult rat brain following kainic acid-induced seizures.
We evaluated the expression of DNA repair proteins, redox factor-1 (Ref-1) and X-ray repair cross-complementing protein 1 (XRCC1), relevant to neurodegeneration following kainic acid-induced seizures in rats. Neurons with oxidative DNA damage exhibited increased expression and colocalization of Ref-1 and XRCC1. Upregulation of DNA repair proteins was also associated with p53 induction and TUNEL. Coexpression of DNA repair proteins and cell death markers following seizures suggests that the DNA repair response may not be sufficient to prevent excitotoxin-induced neurodegeneration.
Quach
Nancy
N
Department of Neurology, UCI School of Medicine, ZOT 4275, 100 Irvine Hall, Irvine, CA 92612-4275, USA.
Chan
Tony
T
Lu
Tony Au
TA
Schreiber
Steven S
SS
Tan
Zhiqun
Z
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D007608:Kainic Acid / Q000633:toxicity; D008297:Male; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D000076105:X-ray Repair Cross Complementing Protein 1
D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C000619372:Xrcc1 protein, rat; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D007608:Kainic Acid