Association mechanism between a series of rodenticide and humic acid: a frontal analysis to support the biological data.
The binding constants (K) of a series of anticoagulant rodenticides with the main soil organic component, humic acid (HA), were determined using frontal analysis approach. The order of the binding constants was identical as the one obtained in a previous paper [J. Chromatogr. B 813 (2004) 295], i.e. bromadiolone>brodifacoum>difenacoum>chlorophacinone>diphacinone, confirming the power of this frontal analysis approach for the determination of binding constants. Moreover, and for the first time, the concentration of unbound rodenticide to HAs could be determined. Thanks this approach, we could clearly demonstrate that HA acid protected the human hepatoma cell line HepG2 against the cytotoxicity of all the rodenticides tested and that the toxicity of rodenticides was directly linked to the free rodenticide fraction in the medium (i.e. unbound rodenticide to HA).
André
Claire
C
Equipe Sciences Séparatives et Biopharmaceutiques (2SB/EA-3124), Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place St. Jacques, 25030 Besançon Cedex, France.
Guyon
Catherine
C
Thomassin
Mireille
M
Barbier
Alexandre
A
Richert
Lysiane
L
Guillaume
Yves-Claude
YC
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006812:Humic Substances; D012378:Rodenticides / Q000737:chemistry* / Q000633:toxicity; D014407:Tumor Cells, Cultured
D000925:Anticoagulants; D006812:Humic Substances; D012378:Rodenticides