Anti-inflammatory activity of CML-1: an herbal formulation.
CML-1 is a purified extract from a mixture of 13 Oriental herbs (Achyranthis Radix, Angelicae Gigantis Radix, Cinnamomi Cortex Spissus, Eucommiae Cortex, Glycyrrhizae Radix, Hoelen, Lycii Fructus, Paeoniae Radix, Rehmanniae Radix Preparata and Atractylodis Rhizoma, Zingiberis Rhizoma, Zizyphi Semen, Acori Graminei Rhizoma) that have been widely used for the treatment of inflammatory diseases in Asia. The aim of this study was to investigate the anti-inflammatory and analgesic potential of CML-1. The animals used in this study were administered either vehicle or CML-1 (30, 100, 300 and 600 mg/kg) orally. The vascular permeability induced by acetic acid was significantly reduced by CML-1 in all doses. The swelling of the rat's hind paw induced by carrageenan was significantly inhibited by CML-1 in doses of 100, 300 and 600 mg/kg. In the case of rheumatoid arthritis induced by complete Freund's adjuvant in rats, the treatment with CML-1 at a dose level of 300 mg/kg inhibited edema. CML-1 at a dose level of 600 mg/kg inhibited acetic acid-induced writhing syndrome, however it did not have any anti-nociceptive action in the Randall-Selitto assay or the hot plate test. Our findings suggest that CML-1 has a potent anti-inflammatory activity.
Eum
Hyun-Ae
HA
College of Pharmacy, Sungkyunkwan University, Suwon 440-746, South Korea.
Lee
Woo-Yong
WY
Kim
Sung-Ho
SH
Kim
Joo-Young
JY
Park
Sang-Won
SW
Lee
Jun-Seok
JS
Choi
Sun-Mi
SM
Pyo
Suhkneung
S
Kang
Myung-Ja
MJ
Min
Yong-Deuk
YD
Shim
Sang-Ho
SH
Shin
Dae-Hee
DH
Lee
Sun-Mee
SM
D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001522:Behavior, Animal; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced; D006614:Hindlimb; D008297:Male; D009619:Nociceptors / Q000187:drug effects*; D017288:Pain Threshold / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D002351:Carrageenan; D019342:Acetic Acid