Transgenic engineering of male-specific muscular hypertrophy.
Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.
Pirottin
Dimitri
D
Unit of Factorial and Molecular Genetics, Department of Animal Production, University of Liège (B43), 20 Boulevard de Colonster, B-4000 Liège, Belgium.
Grobet
Luc
L
Adamantidis
Antoine
A
Farnir
Frédéric
F
Herens
Christian
C
Schrøder
Henrik Daa
HD
Georges
Michel
M
D000818:Animals; D001837:Body Weights and Measures; D001947:Breeding / Q000379:methods; D004268:DNA-Binding Proteins / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development*; D018994:Myosin Light Chains / Q000235:genetics*; D055435:Myostatin; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D051876:Sex-Determining Region Y Protein; D039901:Totipotent Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D014998:Y Chromosome / Q000235:genetics*
D004268:DNA-Binding Proteins; C525912:Mstn protein, mouse; D018994:Myosin Light Chains; D055435:Myostatin; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C076659:myosin light chain I