Cellular uptake of the EBV transcription factor EB1/Zta.
A number of viral proteins have the property to penetrate into the cells when present in the extra-cellular compartment. Here, we report that the Epstein-Barr virus (EBV) transcriptional activator EB1/Zta, which is responsible for the activation of the EBV lytic replication, binds to lymphoid cells surface, is efficiently translocated and accumulates in the nucleus. The internalization of EB1/Zta is energy-dependent and shares common features with endocytosis. As the EB1/Zta was not degraded in the cells and reached the nucleus, the potential effect of its internalisation on viral reactivation was assessed.
Mahot
Ségolène
S
Laboratoire de Virologie Moléculaire et Structurale, FRE CNRS 2854, Faculté de Pharmacie, Domaine de la Merci, 38706 La Tronche Cedex, France.
Fender
Pascal
P
Vivès
Romain R
RR
Caron
Cécile
C
Perrissin
Monique
M
Gruffat
Henri
H
Sergeant
Alain
A
Drouet
Emmanuel
E
D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004705:Endocytosis; D006801:Humans; D018613:Microscopy, Confocal; D011485:Protein Binding; D021381:Protein Transport; D015534:Trans-Activators / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014764:Viral Proteins