Children and adults with acute lymphoblastic leukaemia have similar gene expression profiles.
OBJECTIVE
To compare the gene expression pattern in children and adults with acute lymphoblastic leukaemia (ALL) in order to improve our understanding of the difference in disease biology and prognosis.


METHODS
The gene expression profiles in diagnostic samples from 29 children and 15 adults with ALL were analysed using the oligonucleotide chip Hu95ver2a, produced by Affymetrix.


RESULTS
Unsupervised hierarchical cluster analysis revealed that, in spite of differences in outcome, patients clustered irrespective of age, first by T-cell or B-precursor immunophenotype, and second by cytogenetic changes within the B-precursor group. The expression pattern analysis allowed the reclassification of some samples into the proper cytogenetic group. We also showed that separate clustering of samples with the BCR/ABL translocation could be explained by different breakpoint regions in the BCR. No significant difference in gene expression was observed between samples with and without CDKN2A deletion within the B-precursor group. Analysis of different age groups revealed a similarity in expression profiles when infants with the MLL translocation and adults over 40 yr of age were compared irrespective of karyotype.


CONCLUSIONS
In spite of the difference in clinical outcome, the gene expression pattern in children and adults with ALL is very similar and is primarily dependent on immunophenotype and cytogenetic aberrations. However, when age groups are compared, the expression patterns of infants and adults over 40 show a remarkable similarity.
Kuchinskaya
E
E
Department of Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
Heyman
M
M
Grandér
D
D
Linderholm
M
M
Söderhäll
S
S
Zaritskey
A
A
Nordgren
A
A
Porwit-Macdonald
A
A
Zueva
E
E
Pawitan
Y
Y
Corcoran
M
M
Nordenskjöld
M
M
Blennow
E
E
D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D019942:Genes, p16; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*
D016044:Fusion Proteins, bcr-abl