A gating mechanism proposed from a simulation of a human alpha7 nicotinic acetylcholine receptor.
The nicotinic acetylcholine receptor is a well characterized ligand-gated ion channel, yet a proper description of the mechanisms involved in gating, opening, closing, ligand binding, and desensitization does not exist. Until recently, atomic-resolution structural information on the protein was limited, but with the production of the x-ray crystal structure of the Lymnea stagnalis acetylcholine binding protein and the EM image of the transmembrane domain of the torpedo electric ray nicotinic channel, we were provided with a window to examine the mechanism by which this channel operates. A 15-ns all-atom simulation of a homology model of the homomeric human alpha7 form of the receptor was conducted in a solvated palmitoyl-2-oleoyl-sn-glycerol-phosphatidylcholine bilayer and examined in detail. The receptor was unliganded. The structure undergoes a twist-to-close motion that correlates movements of the C loop in the ligand binding domain, via the beta10-strand that connects the two, with the 10 degrees rotation and inward movement of two nonadjacent subunits. The Cys loop appears to act as a stator around which the alpha-helical transmembrane domain can pivot and rotate relative to the rigid beta-sheet binding domain. The M2-M3 loop may have a role in controlling the extent or kinetics of these relative movements. All of this motion, along with essential dynamics analysis, is suggestive of the direction of larger motions involved in gating of the channel.
Law
Richard J
RJ
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA. rlaw@mccammon.ucsd.edu
Henchman
Richard H
RH
McCammon
J Andrew
JA
D001665:Binding Sites; D002244:Carbon / Q000737:chemistry; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry; D008958:Models, Molecular; D010713:Phosphatidylcholines / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011978:Receptors, Nicotinic / Q000737:chemistry*; D012984:Software; D064569:alpha7 Nicotinic Acetylcholine Receptor
C579167:Chrna7 protein, human; D008024:Ligands; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011506:Proteins; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D002244:Carbon; D003545:Cysteine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine