title: Adaptation of Saccharomyces cerevisiae to high hydrostatic pressure causing growth inhibition.
abstract: Genome-wide mRNA expression profiles of Saccharomyces cerevisiae growing under hydrostatic pressure were characterized. We selected a hydrostatic pressure of 30 MPa at 25 degrees C because yeast cells were able to grow under these conditions, while cell size and complexity were increased after decompression. Functional characterization of pressure-induced genes suggests that genes involved in protein metabolism and membrane metabolism were induced. The response to 30 MPa was significantly different from that observed under lethal conditions because protein degradation was not activated under 30 MPa pressure. Strongly induced genes those that contribute to membrane metabolism and which are also induced by detergents, oils, and membrane stabilizers.
authors.affiliation: Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan. hitoshi.iwahashi@aist.go.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Mine
authors.initials: M
authors.lastname: Odani
authors.forename: Emi
authors.initials: E
authors.lastname: Ishidou
authors.forename: Emiko
authors.initials: E
authors.lastname: Kitagawa
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D017931:DNA Primers; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D011312:Pressure; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D017931:DNA Primers; D012331:RNA, Fungal; D012333:RNA, Messenger