A peptide preparation protects cells in organotypic brain slices against cell death after glutamate intoxication.
Cerebrolysin has been shown to have neurotrophic and neuroprotective potential similar to NGF or BDNF. In the present study organotypic brain slices were utilized to determine the neuroprotective effects of Cerebrolysin, in a glutamate lesion paradigm mimicking a key event in ischemia. The study focused on the effects of Cerebrolysin on both necrotic and apoptotic cell death. Two specific DNA intercalating dyes were used to distinguish the type of cell death. The drug effect was evaluated both microscopically and quantitatively before, 24 hours after and then again 8 days after the lesion. Cerebrolysin was added either before and after the lesion or after the lesion only. The most pronounced effect was seen with the drug added both prior to and after the glutamate lesioning. A treatment after the lesion only also counteracted necrosis and apoptosis. The results render the drug relevant for treating acute as well as chronic neurodegenerative diseases.
Riley
C
C
JSW-Research, Institute of Experimental Pharmacology, Graz, Austria.
Hutter-Paier
B
B
Windisch
M
M
Doppler
E
E
Moessler
H
H
Wronski
R
R
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D018698:Glutamic Acid / Q000633:toxicity*; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010455:Peptides / Q000494:pharmacology*
D000596:Amino Acids; D018696:Neuroprotective Agents; D010455:Peptides; C006952:cerebrolysin; D018698:Glutamic Acid