[Study on the drug resistance and beta-lactamases of Pseudomonas aeruginosa].
OBJECTIVE
To analyze the resistance and beta-lactamases of 93 strains of Pseudomonas aeruginosa.


METHODS
Antimicrobial activity to 93 strains of Pseudomonas aeruginosa were determined by K-B test, and the beta-lactamases produced by these strains were characterized by isoelectric focusing, three-dimensional test, 2-mercaptopropanoic acid inhibited assays, and polymerase chain reaction (PCR).


RESULTS
The resistance of amikacin, cefepime, cefoperazone/sulbactam, ceftazidime, meropenem were 82.8%, 81.7%, 81.7%, 80.6%, 77.4%, respectively. The susceptibility of rate imipenem was 71.0%. Three-dimensional test showed that 14 strains produced carbapenemases, only and 2 strain produced metallo-beta-lactamase, which was in agreement with the result of 2-mercaptopropanoic acid inhibited assays, 13 strains produced AmpC enzymes, and 2 strains produced AmpC enzymes and ESBLs. The 15 strains and other two (Number 19, 54) had 1 band on IEF electrophoresis, and the isoelectric points were 9.0 and 6.3 respectively. The 2 metallo-beta-lactamases were revealed by amplified PCR products with VIM-2 specific PCR, the production was sequenced and revealed that the found to match with the known sequences of VIM-2.


CONCLUSIONS
The production of carbapenemases and AmpC enzymes was the main cause for multi-drug resistance in Pseudomonas aeruginosa in our hospital.
Wang
Jie
J
Department of Respiratory Medicine, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
Zhou
Jian-ying
JY
Yu
Yun-song
YS
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases