RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells.
SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference.
Duxbury
Mark S
MS
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Ashley
Stanley W
SW
Whang
Edward E
EE
D000255:Adenosine Triphosphate / Q000172:deficiency / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D006801:Humans; D008322:Mammals; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D034622:RNA Interference; D034443:RNA Transport / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D034741:RNA, Small Interfering; C452203:SID-1 protein, C elegans; C571194:systemic RNA Interference-defective-1 transmembrane family member 1, human; D000255:Adenosine Triphosphate