title: Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques.
abstract: The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluorescence in situ hybridization, PCR, denaturing gradient gel electrophoresis, and sequencing were used to track changes in bacterial biofilm populations treated with nitrate, nitrite, or nitrate+molybdate as agents for the control of sulfide production. Results indicated that nitrite and nitrate+molybdate reduced sulfide production, while nitrate alone had no effect on sulfide generation. No long-term effect on sulfide production was observed. Initial sulfate-reducing bacterial numbers were not influenced by the chemical treatments, although a significant increase in sulfate-reducing bacteria was observed after termination of the treatments. Molecular analysis showed a diverse bacterial population, but no major shifts in the population due to treatment effects were observed.
authors.affiliation: The Danish Technological Institute, Teknologiparken, Kongsvangs Alle 29, Aarhus, 8000, Denmark.
authors.forename: B V
authors.initials: BV
authors.lastname: Kjellerup
authors.forename: R H
authors.initials: RH
authors.lastname: Veeh
authors.forename: P
authors.initials: P
authors.lastname: Sumithraratne
authors.forename: T R
authors.initials: TR
authors.lastname: Thomsen
authors.forename: K
authors.initials: K
authors.lastname: Buckingham-Meyer
authors.forename: B
authors.initials: B
authors.lastname: Frølund
authors.forename: P
authors.initials: P
authors.lastname: Sturman
mesh_terms: D018441:Biofilms / Q000254:growth & development; D019149:Bioreactors; D003470:Culture Media; D004586:Electrophoresis / Q000379:methods; D005648:Fuel Oils; D017404:In Situ Hybridization, Fluorescence; D007220:Industrial Waste; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism / Q000494:pharmacology; D009573:Nitrites / Q000378:metabolism / Q000494:pharmacology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D018505:Waste Management; D014871:Water Microbiology; D014874:Water Pollutants, Chemical
chemical_list: D003470:Culture Media; D005648:Fuel Oils; D007220:Industrial Waste; D009566:Nitrates; D009573:Nitrites; D013440:Sulfides; D014874:Water Pollutants, Chemical; C044659:molybdate; D008982:Molybdenum