Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium.
Fully deuterated components from autotrophic cell lysate are useful materials for labeling of heterotrophs with deuterium. To facilitate the faster production of deuterated algal lysate, we selected a mutant Chlorella strain that grows faster in heavy water than the wild type. The mutant DR-17 was found to have a higher level of Hsp60 and an elevated level of protein synthesis. We previously isolated a deuterium-resistant yeast cell line that was also found to express elevated level of Hsp70 (K. Unno, T. Kishido, M. Morioka, S. Okada, and N. Oku, Biol. Pharm. Bull. 26:799-802, 2003). This suggests that the overexpression of heat shock proteins is required to compensate for the deuterium isotope effect.
Unno
Keiko
K
Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. unno@u-shizuoka-ken.ac.jp
Hagima
Naoko
N
Kishido
Takahiro
T
Okada
Shoji
S
Oku
Naoto
N
D002460:Cell Line; D018834:Chaperonin 60 / Q000096:biosynthesis*; D002708:Chlorella / Q000378:metabolism*; D003470:Culture Media; D041961:Deuterium Exchange Measurement; D017666:Deuterium Oxide / Q000378:metabolism*
D018834:Chaperonin 60; D003470:Culture Media; D017666:Deuterium Oxide