title: Models of the behavior of Escherichia coli O157:H7 in raw sterile ground beef stored at 5 to 46 degrees C.
abstract: Escherichia coli O157:H7 can contaminate raw ground beef and cause serious human foodborne illness. Previous reports describe the behavior of E. coli O157:H7 in ground beef under different storage conditions; however, models are lacking for the pathogen's behavior in raw ground beef stored over a broad range of temperature. Using sterile irradiated raw ground beef, the behavioral kinetics of 10 individual E. coli O157:H7 strains and/or a 5- or 10-strain cocktail were measured at storage temperatures from 5 degrees to 46 degrees C. Growth occurred from 6 to 45 degrees C. Although lag phase duration (LPD) decreased from 10.5 to 45 degrees C, no lag phase was observed at 6, 8, or 10 degrees C. The specific growth rate (SGR) increased from 6 to 42 degrees C then declined up to 45 degrees C. In contrast to these profiles, the maximum population density (MPD) declined with increasing temperature, from approximately 9.7 to 8.2 log cfu/g. Bias (B(f)) and accuracy (A(f)) factors for an E. coli O157:H7 broth-based aerobic growth model (10 to 42 degrees C) applied to the observations in ground beef were 1.05, 2.70, 1.00 and 1.29, 2.87, 1.03, for SGR, LPD and MPD, respectively. New secondary models increased the accuracy of predictions (5 to 45 degrees C), with B(f) and A(f) for SGR, LPD, and MPD of 1.00, 1.06, and 1.00 and 1.14, 1.33, and 1.02, respectively. These new models offer improved tools for designing and implementing food safety systems and assessing the impact of E. coli O157:H7 disease.
authors.affiliation: Microbial Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. mtamplin@errc.ars.usda.gov
authors.forename: Mark L
authors.initials: ML
authors.lastname: Tamplin
authors.forename: Greg
authors.initials: G
authors.lastname: Paoli
authors.forename: Benne S
authors.initials: BS
authors.lastname: Marmer
authors.forename: John
authors.initials: J
authors.lastname: Phillips
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D019453:Escherichia coli O157 / Q000254:growth & development*; D005511:Food Handling / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008461:Meat Products / Q000382:microbiology*; D008954:Models, Biological; D013696:Temperature