[Application of the nucleotidic sequencing of VP1 to the identification of human Enterovirus].
The introduction of a mollecular method to identify the Entoviruses based on the amplification sequecing and phylogenetic analysis of protein VPI was described. It was proved that this method reduces significantly the time required for the identification of the isolated Entoviruses and that it is very useful in the characterization of isolates which are difficult to typify by the routine immunoloigical reagents. As it is a very fast technqiue, its use is very important during epidemics to determine the causal agent rapidly.
Barrios Olivera
Julio Antonio
JA
Instituto de Medicina Tropical "Pedro Kouri", Ciudad de La Habana. ciipk@ipk.sld.cu
Pérez
Luis Sarmiento
LS
Valdés
Odalys
O
Lago
Pedro Mas
PM
Puentes Palomera
Rosa
R
D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D015678:Viral Structural Proteins / Q000235:genetics*
D015678:Viral Structural Proteins