title: Simultaneous determination of unmodified sevoflurane and of its metabolite hexafluoroisopropanol in urine by headspace sorptive extraction-thermal desorption-capillary gas chromatography-mass spectrometry.
abstract: Unmodified sevoflurane and its metabolite, hexafluoroisopropanol (HFIP), have both been proposed as biomarkers of exposure in post-shift urine for operating room personnel exposed to inhalation anaesthetic sevoflurane. We used headspace sorptive extraction (HSSE) and thermal desorption-capillary GC-MS to assess sensitively both compounds in the urine matrix (after a HFIP deconjugation step). In GC-MS splitless mode, calibration plots (approximately 15-650 microg/L) were linear (r2 > 0.9910) and the limits of detection (1 microg/L for both biomarkers) showed increased sensitivity for HFIP with respect to the previously described headspace GC-MS method. The method was suitable for biological monitoring of both biomarkers of exposure to sevoflurane.
authors.affiliation: Safety, Hygiene and Occupational Medicine Service, University of Bologna, Via Pelagio Palagi 9, Bologna 40138, Italy. antonio.accorsi@unibo.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Accorsi
authors.forename: Barbara
authors.initials: B
authors.lastname: Morrone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Benzo
authors.forename: Carlo
authors.initials: C
authors.lastname: Gandini
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: Raffi
authors.forename: Francesco Saverio
authors.initials: FS
authors.lastname: Violante
mesh_terms: D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008738:Methyl Ethers / Q000652:urine*; D020005:Propanols / Q000652:urine*; D012680:Sensitivity and Specificity; D000077149:Sevoflurane; D006845:Hydrocarbons, Fluorinated
chemical_list: D008738:Methyl Ethers; D020005:Propanols; D000077149:Sevoflurane; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated