title: [Detection of Mobiluncus species by special primer amplification and 16S DNA sequence analysis].
abstract: Bacterial Vaginosis (BV), a microecological disease leaded by overgrowth of the vaginal bacteria, is one of the Polymicrobial Diseases. The close relationship between BV and Mobiluncus ssp. was recognized gradually. But it is difficult to get the pure culture of this anaerobic bacterium because of its rigorous requirement for growth conditions. The vaginal discharge came from the BV animal model--Rhesus monkey was cultured in anaerobic environment. The 16S rRNA gene was amplified and sequenced using mobiluncus-specific primers. Mobiluncus ssp, closely related to Mobiluncus mulieris, were detected, by comparing with the 16S rRNA genes in the GenBank.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences , Beijing 100080, China. lianghe@mail.im.ac.cn
authors.forename: Liang
authors.initials: L
authors.lastname: He
authors.forename: Qun
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhong-ming
authors.initials: ZM
authors.lastname: Zeng
authors.forename: Zhi-heng
authors.initials: ZH
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D005260:Female; D008253:Macaca mulatta; D017904:Mobiluncus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014621:Vagina / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S