Issues for consideration in the analysis of microarray data in behavioural studies.
Microarrays are one of several technologies that allow for measurement the expression of thousands of genes simultaneously. This technological advance provides a challenge for the analysis of these data. In this review we discuss these analytical issues from the initial quality control to normalization, differential expression, clustering and finally functional pathway analysis. We focus on Affymetrix data but many of the issues are the same for other array platforms.
Barr
Gordon
G
Department of Developmental Psychobiology, New York State Psychiatry Institute, NY 10032, USA. gab5@columbia.edu
Gao
Puhong
P
D001525:Behavioral Sciences / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D018076:DNA, Complementary; D012313:RNA