Second harmonic imaging of exocytosis at fertilization.
Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time.
Millard
Andrew C
AC
Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030-1507, USA.
Terasaki
Mark
M
Loew
Leslie M
LM
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D005089:Exocytosis / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005456:Fluorescent Dyes; D047331:Lytechinus / Q000502:physiology; D008297:Male; D011726:Pyridinium Compounds
C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds