Droplet-based micro-flow chemiluminescence system for in vivo glucose determination by microdialysis sampling.
A micro-flow chemiluminescence (CL) system in vivo for glucose determination by the on-line microdialysis sampling is described in this paper. The micro-flow CL system uses discrete sample droplets, which formed at the tip of the capillary with the sampling volume of 4.5 microl. The sol-gel method is introduced to co-immobilize horseradish peroxidase (HRP) and glucose oxidase (GOD) on the inside surface of the micro-flow cell which was fabricated in polymethyl methacrylate (PMMA). The CL detection involved enzymatic oxidation of glucose to D-gluconic acid and H2O2, then H2O2 oxidizing luminol to produce CL in presence of HRP. The microdialysis probe was utilized for sampling in the rabbit blood; the sample throughput was 20 h(-1). The glucose level in blood of the rabbit was on-line monitored with good results.
Liu
Wei
W
Department of Chemistry, Institute of Analytical Science, Southwest Normal University, Beibei, Chongqing 400715, PR China.
Zhang
Zhujun
Z
Liu
Haisheng
H
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D017551:Microdialysis; D011817:Rabbits; D012996:Solutions
D001786:Blood Glucose; D007202:Indicators and Reagents; D012996:Solutions; D008165:Luminol; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase