title: Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis.
abstract: We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem + monolith; microsystem + nib) and their test before the fabrication and testing of fully integrated microsystems.
authors.affiliation: Université des Sciences et Technologies de Lille (Lille 1), Laboratoire de Chimie Organique et Macromoléculaire, UMR CNRS 8009, Bâtiment C4, 2eme etage, 59655 Villeneuve d'Ascq, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Carlier
authors.forename: Steve
authors.initials: S
authors.lastname: Arscott
authors.forename: Vincent
authors.initials: V
authors.lastname: Thomy
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Camart
authors.forename: Cécile
authors.initials: C
authors.lastname: Cren-Olivé
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Gac
mesh_terms: D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018511:Systems Integration