C-fos expression of osteoblast-like MC3T3-E1 cells induced either by cooling or by fluid flow.
Fluid flow is one of the most potent mechanical stimulators for bone cells. Recent reports suggest that primary cilia as well as ion channels and related molecules play roles in the flow detection by kidney epithelial cells. We asked if there is any commonality between kidney and bone cells in flow detection. Primary cilia of cultured osteoblastic cell line MC3T3-E1 were detected by fluorescence staining of acetylated alpha-tubulins. The cells responded to fluid flow, generated by manual rocking of flasks, and expressed c-fos gene. Moreover, the cells were found to respond not only the flow but also cooling of the culture fluid, with a simple technique to keep temperature precisely. It was suggested that bone and kidney cells might share certain similarity in flow detection mechanisms.
Takaoki
Muneo
M
Space Environment Utilization Research Center, ISAS, JAXA.
Murakami
Naoko
N
Gyotoku
Jun-Ichiro
J
D000818:Animals; D002478:Cells, Cultured; D002923:Cilia / Q000502:physiology; D015870:Gene Expression; D016762:Genes, fos; D007668:Kidney / Q000166:cytology; D051379:Mice; D009038:Motion; D010006:Osteoblasts / Q000502:physiology; D013314:Stress, Mechanical; D013696:Temperature