The probability distribution for a random match between an experimental-theoretical spectral pair in tandem mass spectrometry.
Proteomic techniques are fast becoming the main method for qualitative and quantitative determination of the protein content in biological systems. Despite notable advances, efficient and accurate analysis of high throughput proteomic data generated by mass spectrometers remains one of the major stumbling blocks in the protein identification problem. We present a model for the number of random matches between an experimental MS-MS spectrum and a theoretical spectrum of a peptide. The shape of the probability distribution is a function of the experimental accuracy, the number of peaks in the experimental spectrum, the length of the interval over which the peaks are distributed, and the number of theoretical spectral peaks in this interval. Based on this probability distribution, a goodness-of-fit tool can be used to yield fast and accurate scoring schemes for peptide identification through database search. In this paper, we describe one possible implementation of such a method and compare the performance of the resulting scoring function with that of SEQUEST. In terms of speed, our algorithm is roughly two orders of magnitude faster than the SEQUEST program, and its accuracy of peptide identification compares favorably to that of SEQUEST. Moreover, our algorithm does not use information related to the intensities of the peaks.
Fridman
Tema
T
Joint Institute for Computer Science, University of Tennessee/ORNL, ORNL, PO Box 2008, Oak Ridge, TN 37831-6164, USA. fridmant@ornl.gov
Razumovskaya
Jane
J
Verberkmoes
Nathan
N
Hurst
Greg
G
Protopopescu
Vladimir
V
Xu
Ying
Y
D000465:Algorithms; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D015233:Models, Statistical; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods*; D016008:Statistical Distributions
D010455:Peptides; D011506:Proteins