title: Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS.
abstract: To identify genetic factors contributing to psoriasis susceptibility, gene expression profiles of uninvolved epidermis from psoriatic patients and epidermis from healthy individuals were compared. Besides already characterized genes, we identified a cDNA with yet unknown functions, which we further characterized and named PRINS (Psoriasis susceptibility-related RNA Gene Induced by Stress). In silico structural and homology studies suggested that PRINS may function as a noncoding RNA. PRINS harbors two Alu elements, it is transcribed by RNA polymerase II, and it is expressed at different levels in various human tissues. Real time reverse transcription-PCR analysis showed that PRINS was expressed higher in the uninvolved epidermis of psoriatic patients compared with both psoriatic lesional and healthy epidermis, suggesting a role for PRINS in psoriasis susceptibility. PRINS is regulated by the proliferation and differentiation state of keratinocytes. Treatment with T-lymphokines, known to precipitate psoriatic symptoms, decreased PRINS expression in the uninvolved psoriatic but not in healthy epidermis. Real time reverse transcription-PCR analysis showed that stress signals such as ultraviolet-B irradiation, viral infection (herpes simplex virus), and translational inhibition increased the RNA level of PRINS. Gene-specific silencing of PRINS by RNA interference revealed that down-regulation of PRINS impairs cell viability after serum starvation but not under normal serum conditions. Our findings suggest that PRINS functions as a noncoding regulatory RNA, playing a protective role in cells exposed to stress. Furthermore, elevated PRINS expression in the epidermis may contribute to psoriasis susceptibility.
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Szeged 6720, Hungary.
authors.forename: Eniko
authors.initials: E
authors.lastname: Sonkoly
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bata-Csorgo
authors.forename: Andor
authors.initials: A
authors.lastname: Pivarcsi
authors.forename: Hilda
authors.initials: H
authors.lastname: Polyanka
authors.forename: Anna
authors.initials: A
authors.lastname: Kenderessy-Szabo
authors.forename: Gergely
authors.initials: G
authors.lastname: Molnar
authors.forename: Karoly
authors.initials: K
authors.lastname: Szentpali
authors.forename: Lilla
authors.initials: L
authors.lastname: Bari
authors.forename: Klara
authors.initials: K
authors.lastname: Megyeri
authors.forename: Yvette
authors.initials: Y
authors.lastname: Mandi
authors.forename: Attila
authors.initials: A
authors.lastname: Dobozy
authors.forename: Lajos
authors.initials: L
authors.lastname: Kemeny
authors.forename: Marta
authors.initials: M
authors.lastname: Szell
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D003001:Cloning, Molecular; D017931:DNA Primers; D004817:Epidermis / Q000378:metabolism; D020868:Gene Silencing; D020022:Genetic Predisposition to Disease; D006801:Humans; D014176:Protein Biosynthesis; D011565:Psoriasis / Q000235:genetics*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C504070:PRINS RNA, human; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated