Effect of iontophoresis on skin permeation of defibrase.
OBJECTIVE
To investigate the effect of iontophoresis on skin permeation of defibrase.


METHODS
Iontophoresis was carried out in side-by-side chambers, excised rat skin membrane (RSM) or human epidermis membrane (HEM). The effects of electrode polarity, permeation medium pH and ionic strength were evaluated.


RESULTS
Permeation of defibrase caused by anodal iontophoresis was more effective [the apparent permeability coefficient was (1.2 +/- 0.4) x 10(-4) cm x h(-1)] than that of cathodal iontophoresis [(4.3 +/- 1.4) x 10(-5) cm x h(-1)]. The amount of permeated defibrase caused by anodal iontophoresis in pH 7.4 medium was (25 +/- 5) x 10(-14) mol x cm(-2), which was higher than that of in pH 6. 4 permeation medium [(15 +/- 4) x 10(-14) mol x cm(-2)].


CONCLUSIONS
Iontophoresis could enhance skin permeation of defibrase. Electroosmotic flow effect played an important role.
Zhao
Hui-ying
HY
School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. hyzhao@mail.buct.edu.cn
Yuan
Qi-peng
QP
Zheng
Jun-min
JM
D000818:Animals; D012103:Batroxobin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004817:Epidermis / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007478:Iontophoresis; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption
D005343:Fibrinolytic Agents; D012103:Batroxobin