Antioxidative effect of a chymotrypsin inhibitor from Momordica cochinchinensis (Cucurbitaceae) seeds in a primary rat hepatocyte culture.
The antioxidative activity of a chymotrypsin-specific potato type I inhibitor from Momordica cochinchinensis (MCoCI) (Cucurbitaceae) has been investigated using the primary rat hepatocyte system. tert-Butyl hydroperoxide (t-BHP) was used to induce oxidative stress. Pretreatment of hepatocytes with MCoCI for 24 h significantly reversed t-BHP-induced cell damage, and the associated glutathione depletion and lipid peroxidation. The activities of glutathione-S-transferase and superoxide dismutase were also increased. These results suggested that MCoCI possessed antioxidative activity which may account for some of the pharmacological effects of Momordica cochinchinensis seeds, the traditional Chinese medicine known as Mubiezhi, from which MCoCI was isolated.
Tsoi
Alex Yuen-Kam
AY
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Ng
Tzi-Bun
TB
Fong
Wing-Ping
WP
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D029775:Momordica / Q000737:chemistry; D051381:Rats; D012639:Seeds / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000975:Antioxidants; D015842:Serine Proteinase Inhibitors; D020122:tert-Butylhydroperoxide; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D002918:Chymotrypsin