[Detection of DNA damages of peripheral white blood cells in benzene-exposed workers].
OBJECTIVE
To investigate the effect of benzene exposure on DNA damage of the peripheral white blood cells and to assess the possible dose-response relationship between benzene and DNA damage.


METHODS
Personal benzene exposure was sampled with 3M organic vapor monitors. The time weighted average concentration (8h-TWA) and the cumulative dose were calculated. Single cell gel electrophoresis assay was used to detect DNA damage in white blood cells of benzene-exposed workers. The Olive tail moment and the grade of DNA breakage were used to measure DNA damage.


RESULTS
The Olive tail moment and the grade of DNA breakage in benzene exposure groups were significantly increased in comparison with those in the control group (F = 30.03, P < 0.0001, chi2 = 239.9, P < 0.0001, respectively) and showed a dose-response relationship with benzene concentration. Correlation analysis showed that Olive tail moment was correlated with benzene exposure concentration.


CONCLUSIONS
Benzene exposure resulted in an increase of DNA damage of the peripheral white blood cell, which was in a dose-response relationship manner; cumulative dose was better than simply concentration to reflect benzene exposure.
Xing
Cai-Hong
CH
Institute of Occupational Health and Poison Control, China Center for Disease Control and Prevention, Beijing 100050, China.
Ji
Zhi-Ying
ZY
Li
Gui-Lan
GL
Gao
Yun
Y
D000328:Adult; D001554:Benzene / Q000009:adverse effects*; D004249:DNA Damage; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D055815:Young Adult
D001554:Benzene