Anti-hepatitis C virus effect of citrus unshiu peel and its active ingredient nobiletin.
We investigated the effects of water and ethyl acetate extracts of Citrus unshiu peel (Aurantii Nobilis pericarpium) on hepatitis C virus (HCV) absorption in MOLT-4 cells (a human lymphoblastoid leukemia cell line). By reverse transcription polymerase chain reaction (RT-PCR), we showed that both the ethyl acetate layer of Citrus unshiu peel extract and fraction 7 decreased HCV absorption in MOLT-4 cells. Furthermore, we demonstrated that 3',4',5,6,7,8-hexamethoxyflavone (nobiletin) is the active ingredient that markedly inhibited HCV infection in MOLT-4 cells.
Suzuki
Megumi
M
Department of Pharmacology, School of Medicine, Showa University, Japan.
Sasaki
Kenroh
K
Yoshizaki
Fumihiko
F
Oguchi
Katsuji
K
Fujisawa
Min
M
Cyong
Jong-Chol
JC
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects*; D019698:Hepatitis C, Chronic / Q000188:drug therapy*; D006801:Humans; D015458:Leukemia, T-Cell
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D047309:Flavones; C008661:nobiletin