Antioxidant effects of diarylheptanoid derivatives from Alnus japonica on human LDL oxidation.
Two diarylheptanoids, oregonin (1) and hirsutanone (2), were isolated by bioassay-guided fractionation of the methanol extracts of the leaves of Alnus japonica Steud and their structures were elucidated from their spectroscopic data. Compounds 1 and 2 exhibited significant low-density lipoprotein (LDL)-antioxidant activities, e. g., thiobarbituric acid-reactive substances (TBARS) assay (1: IC50 = 3.2 microM, 2: IC50 = 1.5 microM), lag time (1 : 162 min, 2 : 230 min at 2.0 microM, control: 63 min), relative electrophoretic mobility (REM, inhibition of 1 : 71%, 2 : 75% at 10 microM), apoB-100 fragmentation (inhibition of 1 : 27.3%, 2 : 40.3% at 10 microM) and macrophage-mediated LDL oxidation (inhibition of 1 : 82%, 2 : 84% at 1 microM).
Lee
Woo Song
WS
National Research Lab of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology, Yousong, Daejeon, Republic of Korea.
Kim
Ju-Ryoung
JR
Im
Kyung-Ran
KR
Cho
Kyung-Hyun
KH
Sok
Dai-Eun
DE
Jeong
Tae-Sook
TS
D029661:Alnus; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D008077:Lipoproteins, LDL / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D000975:Antioxidants; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances