title: Clonal spread of pediatric isolates of ciprofloxacin-resistant, emm type 6 Streptococcus pyogenes.
abstract: Twenty-four community isolates of Streptococcus pyogenes resistant to ciprofloxacin and susceptible to levofloxacin, gatifloxacin, and moxifloxacin were studied. Sequence determination of the quinolone resistance-determining regions in the gyrA and parC genes revealed a T/G mutation in parC leading to a Ser79Ala substitution in ParC. All isolates were of the emm type 6, and 18 and 2 of them were indistinguishable or closely related, respectively, on the basis of pulsed-field gel electrophoresis.
authors.affiliation: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco, Paseo de la Universidad, 7 01006-Vitoria-Gasteiz, Spain. oipalmor@vc.ehu.es
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Alonso
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Mateo
authors.forename: Marc
authors.initials: M
authors.lastname: Galimand
authors.forename: Javier
authors.initials: J
authors.lastname: Garaizar
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
authors.forename: Ramón
authors.initials: R
authors.lastname: Cisterna
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002648:Child; D002939:Ciprofloxacin / Q000494:pharmacology*; D017714:Community-Acquired Infections / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006801:Humans; D012703:Serotyping; D013290:Streptococcal Infections / Q000635:transmission*; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D002939:Ciprofloxacin