A fluorescence resonance energy transfer-based probe to monitor nucleosome structure.
Nucleosomes are the basic units of eukaryotic chromatin structure. By restricting factor access to regulatory DNA sequences, nucleosomes significantly impact genomic processes such as transcription, and various mechanisms to alter nucleosome structure to relieve this repression have evolved. Both nucleosomes and processes that alter them are inherently dynamic in nature. Thus, studies of dynamics will be necessary to truly understand these relief mechanisms. We describe here the characteristics of a novel fluorescence resonance energy transfer-based reporter that can clearly signal the formation of a canonical nucleosome structure and follow conformational and compositional changes in that structure, both at the ensemble-average (bulk) and at the single molecule level. Labeled nucleosomes behave conformationally and thermodynamically like typical nucleosomes; thus they are relevant reporters of nucleosome behavior. Nucleosomes and free DNA are readily distinguishable at the single-molecule level. Thus, these labeled nucleosomes are well suited to studies of dynamic changes in nucleosome structure including single-molecule dynamics.
Lovullo
D
D
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Daniel
D
D
Yodh
J
J
Lohr
D
D
Woodbury
N W
NW
D015342:DNA Probes; D004586:Electrophoresis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D013053:Spectrophotometry; D013698:Templates, Genetic
D015342:DNA Probes; D009707:Nucleosomes