[The effect of ultrasonication on immuno-modulating activity of mycelial polysaccharide from Cordyceps gunnii].
OBJECTIVE
To investigate immuno-modulating activity change after mycelial polysaccharide from Cordyceps gunnii (MPCG) was depolymerized by ultrasonication.


METHODS
The effects of MPCG and its depolymerizing mixture on mice spleen lymphocytes proliferation, mice peritoneal macrophage (PMphi) phagocytosis and cytotoxin T lymphocytes (CTL) activity were studied by MTT method, neutral red colorimetry, respectively.


RESULTS
In vitro, MPCG depolymerized by ultrasonication could more significantly inhibite spleen lymphocytes proliferation, PMphi phagocytosis of neutral red and CTL activity in mice than MPCG, in particular at doses of 10 microg/ml and 100 microg/ml. Whereas, four kinds of depolymerized mixture depolymerized for 1h to 2h and 4h to 8h by ultrasonication, respectively, had not evident differences at three various doses.


CONCLUSIONS
MPCG was suitable for depolymerizing for 1h to 2h and its depolymerized mixture had stronger immuno-modulating activity than MPCG.
Xiao
Jianhui
J
Key Lab. of Cell Engineering of Guizhou Province, the Affiliated Hospital of Zunyi Medicinal College, Zunyi 563003.
Liang
Zongqi
Z
Liu
Aiying
A
Wan
Weihong
W
Xiao
Yu
Y
Liu
Jinwei
J
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D032661:Cordyceps; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D025282:Mycelium; D010587:Phagocytosis / Q000187:drug effects*; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*
D007166:Immunosuppressive Agents; D011134:Polysaccharides