title: Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity.
abstract: For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.
authors.affiliation: Toyota Biotechnology & Afforestation Laboratory, Aichi 480-1192, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Saitoh
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ishida
authors.forename: Toru
authors.initials: T
authors.lastname: Onishi
authors.forename: Kenro
authors.initials: K
authors.lastname: Tokuhiro
authors.forename: Eiji
authors.initials: E
authors.lastname: Nagamori
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
authors.forename: Haruo
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D005285:Fermentation; D005818:Genetic Engineering; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D019344:Lactic Acid / Q000096:biosynthesis*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014920:Wine
chemical_list: D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase