title: Immune responses to DNA mismatch repair enzymes hMSH2 and hPMS1 in patients with pancreatic cancer, dermatomyositis and polymyositis.
abstract: To identify tumor antigens useful for diagnosis and immunotherapy of patients with pancreatic ductal adenocarcinoma, we applied a SEREX approach with a cDNA library made from 5 pancreatic cancer cell lines and sera obtained from 8 patients with pancreatic cancer, and isolated total 32 genes, including 14 previously characterized genes and 18 genes with unknown functions. Among these isolated antigens, serum IgG antibodies for 2 isolated DNA mismatch repair enzymes, Homo sapiens mutS homolog 2 (hMSH2) and Homo sapiens postmeiotic segregation increased 1 (hPMS1), were detected in patients with pancreatic ductal adenocarcinoma and dermatomyositis (DM), and polymyositis (PM), but not in sera from healthy individuals. Immunohistochemical study demonstrated that hMSH2 and hPMS1 were over-expressed in pancreatic ductal adenocarcinoma compared to normal pancreatic ducts. These results suggested that hMSH2 and hPMS1 may be useful as CD4+ helper T cell antigens for immunotherapy of pancreatic cancer patients and that serum IgG antibodies may be useful for diagnosis of patients with pancreatic ductal adenocarcinoma and DM/PM.
authors.affiliation: Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Takaho
authors.initials: T
authors.lastname: Okada
authors.forename: Shinobu
authors.initials: S
authors.lastname: Noji
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Goto
authors.forename: Takashi
authors.initials: T
authors.lastname: Iwata
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Fujita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Okada
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Masataka
authors.initials: M
authors.lastname: Kuwana
authors.forename: Michito
authors.initials: M
authors.lastname: Hirakata
authors.forename: Akira
authors.initials: A
authors.lastname: Horii
authors.forename: Seiki
authors.initials: S
authors.lastname: Matsuno
authors.forename: Makoto
authors.initials: M
authors.lastname: Sunamura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D020137:Base Pair Mismatch / Q000235:genetics*; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D003882:Dermatomyositis / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D009363:Neoplasm Proteins / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D017285:Polymyositis / Q000235:genetics*; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; C088996:PMS1 protein, human; D011518:Proto-Oncogene Proteins; C497172:MSH2 protein, human; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein