A new LC/APCI-MS method for the determination of cholesterol oxidation products in food.
Cholesterol oxidation products (COPs) can be formed in the body or in animal foods from cholesterol during food processing. A new method for the extraction and quantification of cholesterol, 7-ketocholesterol, cholestane-3beta-5alpha-6beta-triol, 25-hydroxycholesterol, 5,6alpha-epoxycholesterol, and 7beta-hydroxycholesterol by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry is presented. A baseline separation of all COPs was achieved, allowing a separate quantification also for isobaric compounds. The limits of detection were 15-30 ng/mL, quantification was performed from 100 ng/mL to 10 microg/mL with RSD < 2%. The method was applied successfully to the determination of cholesterol and COPs in processed foods such as pork, beef, chicken, and egg.
Raith
Klaus
K
Institute of Pharmaceutics and Biopharmaceutics, Martin Luther University, Wittenberg, 06120 Halle (S), Germany. raith@pharmazie.uni-halle.de
Brenner
Christian
C
Farwanah
Hany
H
Müller
Gerit
G
Eder
Klaus
K
Neubert
Reinhard H H
RH
D001274:Atmospheric Pressure; D002784:Cholesterol / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002784:Cholesterol