Accelerated embolization healing of aneurysms by polyethylene terephthalate coils seeded with autologous fibroblasts.
OBJECTIVE
The objective of this study was to develop a therapeutic system of aneurysm embolization on the basis of organization of fibrous tissue. The healing process of aneurysms by means of polyethylene terephthalate (PET) fiber coils seeded with autologous fibroblasts was evaluated.


METHODS
The aneurysm was prepared at the common carotid artery of 42 rabbits by end-to-side anastomosis of the jugular vein pouch. PET fiber coils seeded with or without autologous fibroblasts were applied to the aneurysm. Assessment of the tissue appearance and histological examination were performed 3 and 5 weeks after application to evaluate the area embolized by fibrous organization in the aneurysm and the neointima formation at the aneurysm orifice.


RESULTS
An agitated seeding method enabled autologous fibroblasts to attach and proliferate homogeneously on the PET fiber coil. In contrast to the PET fiber coil, the PET fiber coil seeded with autologous fibroblasts accelerated the organization of fibrous tissue in the aneurysm and time to achieve aneurysm embolization. The aneurysm orifice was closed with neointima formed. The aneurysm was histologically embolized with newly formed fibrous tissue, and the area embolized was significantly larger than that of nontreated and the PET fiber coil-treated groups. The orifice was completely covered with endothelial cells.


CONCLUSIONS
The PET fiber coil seeded with autologous fibroblasts is a promising tool for healing aneurysms using embolization therapy by tissue organization.
Kawakami
Osamu
O
Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Miyamoto
Susumu
S
Hatano
Taketo
T
Yamada
Keisuke
K
Hashimoto
Nobuo
N
Tabata
Yasuhiko
Y
D021721:Balloon Occlusion; D002448:Cell Adhesion; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D002532:Intracranial Aneurysm / Q000628:therapy*; D011093:Polyethylene Terephthalates; D014182:Transplantation, Autologous; D014945:Wound Healing
D011093:Polyethylene Terephthalates