title: Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1.
abstract: A hyperthermophilic and aerobic crenarchaeon, Aeropyrum pernix K1, has two sets of genes possibly encoding 2-oxoacid:ferredoxin oxidoreductases. One is encoded in open reading frames (ORFs) ape2126 and ape2128, and the other in ORFs ape1473 and ape1472. The two sets of genes were expressed. The product enzymes, Ape2126/2128 and Ape1473/1472, showed optimal temperatures of 105 and over 110 degrees C, and optimal pHs of 8.5 and 9.0, respectively, using pyruvate as a substrate. Pyruvate, 2-oxobutyrate, and glyoxylate were the best substrates for both enzymes, and additionally Ape1473/1472 was able to act on 2-oxoglutarate, suggesting the enzyme operates in the TCA cycle.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Japan.
authors.forename: Yumie
authors.initials: Y
authors.lastname: Nishizawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yabuki
authors.forename: Eriko
authors.initials: E
authors.lastname: Fukuda
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
mesh_terms: D041522:Aeropyrum / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D004795:Enzyme Stability; D019848:Gene Expression Regulation, Archaeal; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D007658:Ketone Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D019289:Pyruvic Acid; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase