title: [Change of free hemoglobin content after mixing type B whole blood with different amounts of type O whole blood].
abstract: To investigate the changes of free hemoglobin (FHb) content after mixing type B whole blood with different amounts of type O whole blood at room temperature and at 37 degrees C, two lots of type B whole blood stored at 4 degrees C for 24 hours were randomly taken as recipient blood, and were packed as 60 ml respectively. Type O blood was taken as donor blood. 60 ml type B whole bloods were mixed with different amounts of type O whole blood, i.e. with 9, 12, 15 and 18 ml. The mixed blood was packed into 100 ml plastic blood bags and stored at 37 degrees C or room temperature, shaken once every 15 minutes. Free hemoglobin content was determined for the harvested samples at 1, 2, 4, 8 and 12 hours after store. The results showed that there was no significant elevation of FHb within 12 hours after mixing B whole blood with different amounts of type O whole blood. In another lot, there was obvious difference in FHb after 1 hour store along with the prolongation of store at either room temperature or 37 degrees C. In one lot, there was no difference of FHb (P > 0.05) during 1 - 8 hours of store at room temperature or 37 degrees C, but significant difference at 12 hours of store (P < 0.001). In another lot, there was no difference of FHb (P > 0.05) within 1 hour of store at room temperature and at 37 degrees C, but significant difference during 2 approximately 8 hours of store (P < 0.001). It is concluded that the FHb would not change significantly within 12 hours after type B blood was mixed with 1 200 ml of type O whole blood, but when the mixed blood was placed at room temperature or at 37 degrees C for 8 hours, the FHb content approaches, even exceeds 170.4 mg/L which was observed in the blood stored for 2 days. It suggests that freshly collected blood must be put into refrigerator of 2 approximately 4 degrees C for storing as soon as possible, so as to decrease the catabolism of erythrocyte and the releasing of FHb and other metabolites which are deleterious to the recipients.
authors.affiliation: Department of Blood Transfusion, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Xiao
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
authors.forename: Ru-Fu
authors.initials: RF
authors.lastname: Xu
authors.forename: Jing-Han
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D013997:Time Factors
chemical_list: D000017:ABO Blood-Group System; D006454:Hemoglobins