title: Transcriptome analysis of recombinant protein secretion by Aspergillus nidulans and the unfolded-protein response in vivo.
abstract: Filamentous fungi have a high capacity for producing large amounts of secreted proteins, a property that has been exploited for commercial production of recombinant proteins. However, the secretory pathway, which is key to the production of extracellular proteins, is rather poorly characterized in filamentous fungi compared to yeast. We report the effects of recombinant protein secretion on gene expression levels in Aspergillus nidulans by directly comparing a bovine chymosin-producing strain with its parental wild-type strain in continuous culture by using expressed sequence tag microarrays. This approach demonstrated more subtle and specific changes in gene expression than those observed when mimicking the effects of protein overproduction by using a secretion blocker. The impact of overexpressing a secreted recombinant protein more closely resembles the unfolded-protein response in vivo.
authors.affiliation: Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Sims
authors.forename: Manda E
authors.initials: ME
authors.lastname: Gent
authors.forename: Karin
authors.initials: K
authors.lastname: Lanthaler
authors.forename: Nigel S
authors.initials: NS
authors.lastname: Dunn-Coleman
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Oliver
authors.forename: Geoffrey D
authors.initials: GD
authors.lastname: Robson
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D011994:Recombinant Proteins