cda1+, encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe.
In Schizosaccharomyces pombe, a major role of chitin is to build up a complete spore. Here, we analyzed the cda1(+) gene (SPAC19G12.03), which encodes a protein homologous to chitin deacetylases, to know whether it is required for spore formation in S. pombe. The homothallic Deltacda1 strain constructed by homologous recombination was found to form a little amount of abnormal spores that contained one, two, or three asci, similar to (but not as strong as) the phenotype observed in a deletion mutant of chs1 encoding chitin synthase 1. This phenotype is reversed by expression of S. cerevisiae chitin deacetylase CDA1 or CDA2, suggesting that cda1 encodes a chitin deacetylase. To support the role of Cda1 in sporulation, the timing of expression of cda1(+) mRNA increased during sporulation process. We also found that the Cda1 protein self-associated when its binding was tested both by two-hybrid system and immunoprecipitation. Thus, these data indicated that cda1(+) is required for proper spore formation in S. pombe.
Matsuo
Yasuhiro
Y
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
Tanaka
Katsunori
K
Matsuda
Hideyuki
H
Kawamukai
Makoto
M
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D000581:Amidohydrolases; C019866:chitin deacetylase