[Study on AHL-like quorum signals in Mesorhizobium huakuii].
Quorum sensing is a mechanism used by single-cell bacteria to monitor their population density and control a variety of physiological functions in a cell-density-dependent manner. An ultrasensitive quorum signal acyl-homoserine lactone (AHL) detecting Agrobacterium tumefaciens strain, which strongly overproduces quorum-sensing regulator TraR and was proved to be able to detect AHLs at nano-molar concentration, was used to detect the production of AHLs in three Mesorhizobium huakuii strains. We showed that AHLs were produced in these strains with some variations. We also found that elevating of medium pH during cell growth greatly affected the stability of AHL signals. This study paved the way of further studying the relationship between quorum sensing and symbiotic nitrogen fixation of the M. huakuii strains.
Gao
Yi-jing
YJ
Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. yijinggao@yahoo.com.cn
Zhong
Zeng-tao
ZT
Zheng
Hui-ming
HM
Wang
Hui
H
Zhu
Jun
J
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009586:Nitrogen Fixation; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
C088386:homoserine lactone; D015107:4-Butyrolactone