Characterization of different cell culture media for expression of recombinant antibodies in mammalian cells: Presence of contaminating bovine antibodies.
Several new cell culture media designed specifically for the expression of recombinant antibodies in Chinese hamster ovary (CHO) cells were investigated for the presence of bovine IgG. Three serum-free media, three protein-free (animal component free) media, as well as one chemically defined medium were included in the study. Employing a combination of affinity chromatography (Protein G or A columns), SDS-PAGE analysis, and peptide mass fingerprinting, two of the serum-free media were found to contain bovine IgG in the range of approximately 0.5 mg/L. The other five media did not contain detectable levels of contaminating Protein A or G-binding proteins such as bovine IgG.
Rasmussen
Lone Kjaer
LK
Symphogen A/S, DK-2800 Lyngby, Denmark.
Larsen
Yvonne Berger
YB
Højrup
Peter
P
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000737:chemistry; D016895:Culture Media, Serum-Free / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects
D000906:Antibodies; D003470:Culture Media; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D007074:Immunoglobulin G; D011994:Recombinant Proteins