Nucleolonema as a fundamental substructure of the nucleolus.
The nucleolus is the most obvious structure in the eukaryotic nucleus. It is known to be a ribosome-producing apparatus where ribosomal (r) DNA is transcribed and the primary rRNA transcripts are processed to produce three of the four rRNA species. Electron microscopy has shown that the nucleolus consists of three major components, a dense fibrillar component (DFC), a granular component (GC) and a fibrillar center (FC). The DFC and FCs are integrated into a fundamental nucleolar substructure called the nucleolonema. The DFC corresponds to the matrix of the nucleolonema, and the FC is an electron microscopic counterpart of argyrophobic lacunae localized in the nucleolonema. The spherical FCs are intermittently arranged along the length of the nucleolonema in actively growing cells but are fused with each other to form tubular FCs when rDNA transcription is hampered. The RNase-gold complex does not bind to the FC but to the DFC and the GC, suggesting that rDNA transcription does not occur in the FC although both fluorescence in situ hybridization (FISH) and electron microscopic in situ hybridization reveal that the rDNA is specifically localized in the FCs. Immunogold-labeling after bromo-UTP (BrUTP) incorporation shows that rDNA transcription takes place in the boundary region between the FC and the DFC, and primary rRNA transcripts are expected to be processed outward within the DFC. Data have accumulated suggesting that the nucleolonema is a fundamental substructure of the nucleolus, and its skeleton is the tandem arrangement of the FCs, which are resting harbors or storages of rDNA. This paper proposes that the transversal structural organization of the nucleolonema is centrifugally built up by several structural and functional domains: condensed and/or loosened rDNA, rDNA transcription zone, and transcript processing and ribosome assembly zones.
Sato
Seiichi
S
Department of Biology, Faculty of Science, Ehime University, Matsuyama, 790-8577, Japan. ssato@sci.ehime-u.ac.jp
Yano
Hiroyuki
H
Makimoto
Yuji
Y
Kaneta
Tsuyoshi
T
Sato
Yasushi
Y
D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure*; D018744:DNA, Plant / Q000378:metabolism; D004275:DNA, Ribosomal; D018506:Gene Expression Regulation, Plant; D010944:Plants / Q000235:genetics / Q000648:ultrastructure*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D018744:DNA, Plant; D004275:DNA, Ribosomal