Hydrogen, carbon and nitrogen isotopic fractionations during chlorophyll biosynthesis in C3 higher plants.
We determined hydrogen, carbon and nitrogen isotopic compositions of chlorophylls a and b isolated from leaves of five C3 higher plant species (Benthamidia japonica, Prunus japonica, Acer carpinifolium, Acer argutum and Querus mongloica), and hydrogen and carbon isotopic compositions of phytol and chlorophyllides in the chlorophylls to understand isotopic fractionations associated with chlorophyll biosynthesis in these species. Chlorophylls are depleted in D relative to ambient water by approximately 189 per thousand and enriched in (13)C relative to bulk tissue by approximately 1.6 per thousand. These data can be explained by the contribution of isotopic fractionations during phytol and chlorophyllide biosyntheses. Phytol is more depleted in both D (by approximately 308 per thousand) and (13)C (by approximately 4.3 per thousand), while chlorophyllides are less depleted in D (by approximately 44 per thousand) and enriched in (13)C (by approximately 4.8 per thousand). Such inhomogeneous distribution of isotopes in chlorophylls suggests that (1) the phytol in chlorophylls reflects strong D- and (13)C-depletions due to the isotopic fractionations during the methylerythritol phosphate pathway followed by hydrogenation, and (2) the chlorophyllides reflect D- and (13)C-enrichments in tricarboxylic acid cycle. On the other hand, chlorophylls are slightly ( approximately 1.2 per thousand) depleted in (15)N relative to the bulk tissue, indicating that net isotopic fractionation of nitrogen during chlorophyll biosynthesis is small compared with those of hydrogen and carbon.
Chikaraishi
Yoshito
Y
Institute for Research on Earth Evolution (IFREE), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. ychikaraishi@jamstec.go.jp
Matsumoto
Kohei
K
Ogawa
Nanako O
NO
Suga
Hisami
H
Kitazato
Hiroshi
H
Ohkouchi
Naohiko
N
D002247:Carbon Isotopes / Q000378:metabolism*; D002734:Chlorophyll / Q000096:biosynthesis*; D000077194:Chlorophyll A; D002735:Chlorophyllides / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D019684:Magnoliopsida / Q000378:metabolism*; D008956:Models, Chemical; D015394:Molecular Structure; D009587:Nitrogen Isotopes / Q000378:metabolism*; D010836:Phytol / Q000378:metabolism; D014867:Water
D002247:Carbon Isotopes; D002735:Chlorophyllides; D009587:Nitrogen Isotopes; D014867:Water; D002734:Chlorophyll; D010836:Phytol; C037184:chlorophyll b; D006859:Hydrogen; D000077194:Chlorophyll A