[Identification of PCR markers associated with cytoplasmic male sterility in Brassica oleracea var Botrytis].
The homology-based candidate gene method was used to identified the specific PCR markers linked to cytoplasmic male sterility (CMS) in cauliflower( Brassica oleracea var botrytis.). Searching the DNA and protein data-base of NCBI , correlative genes or open reading frames were identified . Analysis of biosoft, based on the conservative regions ,five primers were designed . Among them, only primer P9/P10 produced a 313- bp specific fragment. Identified by individual plant testing , analysis of RT-PCR and dot blot ,this fragment was only existed in CMS cauliflower knxd612. Analysis of the sequence indicated it was high homologous(98%) with orf138 of Ogura CMS radish. Primary result suggested that the cytoplasmic type of CMS cauliflower knxd612 may belong to Ogura type. This research offered a good foundation to further investigate the CMS mechanism of cauliflower in molecular level.
Wang
Chun-Guo
CG
The College of Life Sciences, Nankai University, Tianjin, China. htg1979@eyou.com
Song
Wen-Qin
WQ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001937:Brassica / Q000235:genetics* / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051479:Plant Infertility / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D005819:Genetic Markers