TEL/AML1 and immunoreceptor gene rearrangements-which comes first?
TEL/AML1 fusion gene is present in 20-25% of childhood acute lymphoblastic leukaemias. In order to unravel at which stage of B-cell precursor development the fusion is originated, we analysed frequency and pattern of immunoreceptor (immunoglobulin and T-cell receptor) gene rearrangements in 47 TEL/AML1-positive and 43 TEL/AML1-negative cases of the same CD10+ immunophenotype. Moreover, we compared corresponding immunoreceptor gene rearrangements in 11 cases of TEL/AML1-positive leukaemia at diagnosis and relapse. More mature immunogenotype of TEL/AML1-positive cases and changes in 37% of rearrangements between diagnosis and relapse suggest that in most cases the TEL/AML1 fusion is formed during immunoreceptor gene rearrangement process.
Zuna
Jan
J
CLIP - Childhood Leukaemia Investigation Prague, 2nd Medical School, Charles University Prague, V Uvalu 84, Prague, Czech Republic. jan.zuna@lfmotol.cuni.cz
Krejci
Ondrej
O
Madzo
Jozef
J
Fronkova
Eva
E
Sramkova
Lucie
L
Hrusak
Ondrej
O
Kalina
Tomas
T
Vaskova
Martina
M
Stary
Jan
J
Trka
Jan
J
D001402:B-Lymphocytes / Q000276:immunology; D002453:Cell Cycle / Q000235:genetics; D050676:Core Binding Factor Alpha 2 Subunit; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics; D016130:Immunophenotyping; D015260:Neprilysin / Q000096:biosynthesis / Q000276:immunology; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D012008:Recurrence; D000097764:ETS Translocation Variant 6 Protein
D050676:Core Binding Factor Alpha 2 Subunit; D007136:Immunoglobulins; D015260:Neprilysin; D015514:Oncogene Proteins, Fusion; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein