Association of RANTES with the replication of severe acute respiratory syndrome coronavirus in THP-1 cells.
BACKGROUND
Severe acute respiratory syndrome (SARS) is a novel infectious disease which is characterized by an overaggressive immune response. Chemokines are important inflammatory mediators and regulate disease due to viral infection. In previous study, we found that SARS-CoV has the ability to replicate in mononuclear cells. In present work, we sought to characterize the replication of SARS-CoV at the presence of RANTES in THP-1 cells.


METHODS
To determine whether RANTES play an role in the process of SARS, THP-1 cells were incubated with heat-inactivated SARS-CoV and ELISA was used to test RANTES levels in the supernatants; Then the effect of dexamethasone on the induced secretion was evaluated. Real-time PCR was used to investigate the effort of RANTES on the replication of SARS-CoV in vitro. Macrophages, induced by THP-1 cells, were used as cell model.


RESULTS
Inactive SARS-CoV could induce THP-1 cells secret RANTES and this increase effect could not be suppressed by DXM. RANTES itself could inhibit the replication of SARS-CoV in THP-1 cells when it was added into the culture before or at the same time with the virus; No inhibition effect was shown when RANTES were added into the culture after SARS-CoV infected the cells.
Li
D
D
Department of Dermatology and Allergology, Ruhr-University Bochum, St. Josef-Hospital, Gudrunstr. 56, D-44791 Bochum, Germany.
Wu
N
N
Yao
H
H
Bader
A
A
Brockmeyer
Norbert H
NH
Altmeyer
P
P
D000818:Animals; D000998:Antiviral Agents / Q000502:physiology*; D016466:CHO Cells; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000502:physiology*; D002522:Chlorocebus aethiops; D006224:Cricetinae; D006801:Humans; D008264:Macrophages / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication / Q000502:physiology*
D000998:Antiviral Agents; D018946:Chemokine CCL5