[Usefulness of D-proline in the differentiation of varieties of Cryptococcus neoformans].
A comparative study was conducted between the assimilation of D-proline and the growth on canavanine-glycine-bromothymol blue (CGB) medium used for the classification of the varieties of Cryptococcus neoformans. In the 86 studied strains, 100% of coincidence between both methods allowed to affirm that 95.34% corresponded to the neoformans var. and the rest (4.65%) to the gattii var. The results obtained corroborated that all the autoctonous clinical isolations up to the present correspond to the. neoformans var. and made possible to suggest the use of D-proline for the initial evaluation of strains, as an alternative and simple method that presented under these conditions high coincidence with the reference method (growth in CGB).
Martínez Machín
Gerardo
G
Instituto de Medicina Tropical Pedro Kouri, Apartado 601, Marianao 13, Ciudad de La Habana, Cuba. gerardo@ipk.sld.cu
Barrial de la Rosa
Lisset
L
Illnait Zaragozi
María T
MT
Valdés Hernández
Iliana del C
Idel C
Fernindez Andreu
Carlos M
CM
Perurena Lancha
Mayda R
MR
Polo Leal
Jorge L
JL
Mendoza Llanes
Dianeya
D
D003455:Cryptococcus neoformans / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D011392:Proline / Q000378:metabolism*
D003470:Culture Media; D011392:Proline