The effect of dietary polyunsaturated fatty acids (PUFA) on infection with the nematodes Ostertagia ostertagi and Cooperia oncophora in calves.
Diet-induced changes in the polyunsaturated fatty acid (PUFA) content of immune cells influences the immune phenotype that develops following infection. The aim of this study was to examine the effect of manipulating dietary PUFA supply on tissue fatty acids composition and immunity to a mixed infection with an abomasal and an intestinal nematode parasite in calves. Calves (n=24) were allocated into two treatment groups and fed 25 g/day of either fish oil (n-3 group) or a binary mixture of palm/rapeseed oil (normal group) as a supplement in milk replacer. Within each treatment group eight calves were infected with 2000 L3 Ostertagia ostertagi and Cooperia oncophora, three times per week for 8 weeks, the remaining calves were pair-fed uninfected controls. Faecal egg counts (FEC) were carried out twice weekly. At slaughter, the whole gut was removed intact for worm counts and tissue samples were taken for fatty acid analysis. Samples of abomasum, duodenum and mid-gut were also collected for immunohistological analysis. FEC were not significantly influenced by oil supplement but tended to remain higher in the palm/rapeseed oil-fed group (normal infected). The number of intestinal immature worms was significantly (p<0.05) higher in the n-3 group. Mucosal mast cell (MMC) and eosinophil numbers were significantly increased (p<0.05) by infection and were significantly lower (p<0.05) in the intestinal tissue of the fish oil supplemented and infected group (n-3 infected group). These results suggest that feeding an n-3 PUFA-rich supplement (fish oil) can influence cellular mediators of immunity to nematode infection. This is the first report of the establishment of patency and the subsequent development of immunity to a mixed infection with O. ostertagi and C. oncophora in calves undergoing early rumen development. The trend in the FEC, MMC and eosinophil numbers in the n-3 group suggests that decreasing the dietary n-6/n-3 PUFA ratio may be a worthwhile immunonutritional strategy for potentiating the immune response to nematode parasite infection in the calf.
Muturi
K N
KN
Department of Agriculture and Forestry, School of Biological Sciences, University of Aberdeen, Hilton Campus, Block M, Hilton Place, Aberdeen AB24 4FA, Scotland, UK.
Scaife
J R
JR
Lomax
M A
MA
Jackson
F
F
Huntley
J
J
Coop
R L
RL
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*; D002452:Cell Count / Q000662:veterinary; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004804:Eosinophils / Q000276:immunology / Q000469:parasitology; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005243:Feces / Q000469:parasitology; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000469:parasitology; D007422:Intestines / Q000469:parasitology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000469:parasitology; D010028:Ostertagia / Q000254:growth & development*; D010029:Ostertagiasis / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D000073878:Palm Oil; D010270:Parasite Egg Count / Q000662:veterinary; D010938:Plant Oils / Q000494:pharmacology; D000074262:Rapeseed Oil; D014251:Trichostrongyloidea / Q000254:growth & development*; D014252:Trichostrongyloidiasis / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*
D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3; D010938:Plant Oils; D000074262:Rapeseed Oil; D000073878:Palm Oil