Fingerprint quality control of Tianjihuang by high-performance liquid chromatography-photodiode array detection.
A novel, simple and accurate fingerprint method was developed using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) for the quality control of Hypericum japonicum thunb (Tianjihuang), a Chinese herbal medicine used for the treatment of several bacterial diseases, infectious hepatitis, gastrointestinal disorder, internal hemorrhage and tumors. For the first time, the feasibility and advantages of employing chromatographic fingerprint were investigated and demonstrated for the evaluation of Tianjihuang by systematically comparing chromatograms with a professional analytical software recommended by State Food and Drug Administration (SFDA). Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish raw herbs of Tianjihuang from different sources. The effects resulted from collecting locations, harvesting time and storage time on herbal chromatographic fingerprints were also examined.
Yang
Li-wei
LW
Guangzhou Quality R&D Center of Traditional Chinese Medicine, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.
Wu
Ding-hong
DH
Tang
Xi
X
Peng
Wei
W
Wang
Xiao-rui
XR
Ma
Yan
Y
Su
Wei-wei
WW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D020902:Hypericum / Q000737:chemistry*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*