Effect of Oenanthe javanica flavone on human and duck hepatitis B virus infection.
OBJECTIVE
To study the antiviral effect of Oenanthe javanica flavones (OjF) on human hepatoma HepG2.2.15 culture system and duck hepatitis B virus (DHBV) infection.


METHODS
(1) After incubation for 24 h, the 2.2.15 cells were treated with different concentrations of OjF for 12 d. The cell alteration was observed by microscope. The presence of HBsAg and HBeAg were measured using the enzyme immunoassay kit after 2.2.15 cells were treated with OjF for 9 d. (2) Ducklings infected with DHBV intravenously were divided into 5 groups and treated with OjF, acyclovir (ACV), and normal saline respectively for 10 d. All the ducklings were bled before, during, and after treatments at different times, and serum levels of DHBV-DNA were detected by a dot-blot hybridization assay.


RESULTS
(1) The 50% toxic concentration (TC50) of OjF was 2.28 g/L. The maximum nontoxic concentration (TC0) was 1.00 g/L. In nontoxic concentrations, OjF significantly inhibited HBsAg and HBeAg in 2.2.15 cells after 9 d of treatment (P<0.05, P<0.01). (2) The DHBV-DNA levels decreased significantly after the treatment with 0.50 and 1.00 g/kg of OjF (P<0.01). The inhibition of the peak of viremia was maximum at a dose of 1.00 g/kg and reached 54.3% on d 5 and 64.5% on d 10, respectively.


CONCLUSIONS
The results demonstrate that OjF is a strong inhibitor of HBsAg and HBeAg secretion in 2.2.15 cells and DHBV-DNA levels in the HBV-infected duck model.
Wang
Wei-na
WN
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100084, China.
Yang
Xin-bo
XB
Liu
He-zhi
HZ
Huang
Zheng-ming
ZM
Wu
Guang-xia
GX
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D018347:Hepadnaviridae Infections / Q000821:virology; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000096:biosynthesis; D006524:Hepatitis, Viral, Animal / Q000821:virology; D006801:Humans; D008099:Liver / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology; D031050:Oenanthe / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal; D047309:Flavones; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens