Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel.
The present work evaluates a newly developed silated hydroxypropylmethylcellulose (Si-HPMC)-based hydrogel as a scaffold for 3D culture of osteogenic cells. The pH variation at room temperature catalyzes the reticulation and self-hardening of the viscous polymer solution into a gelatine state. We designed reticulation time, final consistency and pH in order to obtain an easy handling matrice, suitable for in vitro culture and in vivo injection. Three human osteogenic cell lines and normal human osteogenic (HOST) cells were cultured in 3D inside this Si-HPMC hydrogel. We show here that osteosarcoma cells proliferate as clonogenic spheroids and that HOST colonies survive for at least 3 weeks. Mineralization assay and gene expression analysis of osteoblastic markers and cytokines, indicate that all the cells cultured in 3D into this hydrogel, exhibited a more mature differentiation status than cells cultured in monolayer on plastic. This study demonstrates that this Si-HPMC hydrogel is well suited to support osteoblastic survival, proliferation and differentiation when used as a new scaffold for 3D culture and represents also a potential basis for an innovative bone repair material.
Trojani
Christophe
C
FRE 2720 CNRS/UNSA IFR50, Faculté de Médecine, Avenue de Valombrose, 06107 Nice Cedex 02, France.
Weiss
Pierre
P
Michiels
Jean-François
JF
Vinatier
Claire
C
Guicheux
Jérôme
J
Daculsi
Guy
G
Gaudray
Patrick
P
Carle
Georges F
GF
Rochet
Nathalie
N
D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry*; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D065347:Hypromellose Derivatives; D007267:Injections; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D044367:Phase Transition; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D020100:Hydrogels; D065347:Hypromellose Derivatives; D008747:Methylcellulose