title: Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium.
abstract: Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.
authors.affiliation: The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, UK.
authors.forename: John R
authors.initials: JR
authors.lastname: MacFadyen
authors.forename: Oliver
authors.initials: O
authors.lastname: Haworth
authors.forename: David
authors.initials: D
authors.lastname: Roberston
authors.forename: Deborah
authors.initials: D
authors.lastname: Hardie
authors.forename: Marie-Therese
authors.initials: MT
authors.lastname: Webster
authors.forename: Howard R
authors.initials: HR
authors.lastname: Morris
authors.forename: Maria
authors.initials: M
authors.lastname: Panico
authors.forename: Mark
authors.initials: M
authors.lastname: Sutton-Smith
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Peter
authors.initials: P
authors.lastname: van der Geer
authors.forename: Dirk
authors.initials: D
authors.lastname: Wienke
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
authors.forename: Clare M
authors.initials: CM
authors.lastname: Isacke
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers / Q000378:metabolism*; D019556:COS Cells; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004730:Endothelium, Vascular / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D020286:Pericytes / Q000378:metabolism; D011233:Precipitin Tests; D017154:Stromal Cells / Q000378:metabolism*; D013388:Succinimides; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; C078203:CD248 protein, human; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D013388:Succinimides