Correlation between adjuvanticity and immunogenicity of cholera toxin B subunit in orally immunised young chickens.
The aim of the present study was to investigate whether the adjuvanticity of the cholera toxin B (CTB) subunit was correlated with its immunogenicity in young orally immunised chickens. Thirteen 15-day-old chickens were orally immunised with bovine serum albumin (BSA) glutaraldehyde coupled to CTB. The chicken antibody (IgG) concentrations against BSA and CTB, respectively, were quantified by ELISA. A significant positive correlation (r=0.66, n=39, p<0.001) between the concentrations of immunospecific antibodies with specificities against BSA and CTB, respectively, demonstrated that the adjuvanticity of CTB is correlated with its immunogenicity.
Mayo
Susan
S
Department of Neuroscience, Division of Comparative Medicine, Uppsala University, Uppsala, Sweden.
Royo
Felix
F
Hau
Jann
J
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002645:Chickens; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology*; D005976:Glutaral / Q000276:immunology; D007114:Immunization / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013045:Species Specificity
D000276:Adjuvants, Immunologic; D000906:Antibodies; D012710:Serum Albumin, Bovine; D002772:Cholera Toxin; D005976:Glutaral