title: [Lymphocytes T gammadelta receptors in hypertrophied adenoids in children with otitis media with effusion].
abstract: BACKGROUND
  The pharyngeal tonsil is the place of immunological system first contact with pathogenic factors in the nasopharyngeal region, which is responsible for local defence from infection. Chronic antigenic stimulation can lead to adenoid dysfunction and hypertrophy (PMG). Significant role in PMG and in the otitis media with effusion (WZU) play lymphocytes T (CD3(+)) and T-TCR gammadelta. THE AIM of the study was a quantitative analysis of lymphocytes T (CD3(+)) and T-TCR gammadelta in adenoid hypertrophy and otitis media with effusion lasting more than 3 months.
  
  
  METHODS
  An investigation was executed by flow cytometry in hypertrophied adenoids from children after tonsillectomy. The study and control groups were divided in to 2 subgroups: children younger than 5 years and those aged from 5 to 17 years.
  
  
  RESULTS
  The study showed a statistically significant reduction from 35.6+/-3.1% to 32.0+/-2.8% (p < 0.04) of lymphocytes T gammadelta CD3(+) in the examined group (WZU) compared to the control group (PMG). There was a higher percentage of lymphocytes with TCR gammadelta receptor expression in children with WZU (2.04+/-1.13%) than in PMG group (0.99+/-0.44%) (p < 0.002). The lowest percentage of lymphocytes T-TCR gammadelta was found in younger children with PMG (0.67+/-0.17%) and the highest in WZU in younger children (2.13+/-1.04%) (p < 0.0001). We observed a statistically significant difference in expression of TCR receptors (p < 0.02) between younger children with PMG (0.67+/-0.17%) and older children with PMG (1.24+/-0.43%). We did not find significant differences in lymphocytes T CD3(+) related to age.
  
  
  CONCLUSIONS
  The enlarged percentage of lymphocytes T gammadelta in adenoid hypertrophy in children with WZU create a possibility of a more rapid response to inflammatory factors.
authors.affiliation: Zakład Laboratoryjnej Diagnostyki Pediatrycznej, Akademia Medyczna, Waszyngtona 17, 15-296 Białystok, Poland. pedlab@amb.edu.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wysocka
authors.forename: Bozena
authors.initials: B
authors.lastname: Skotnicka
authors.forename: Karol
authors.initials: K
authors.lastname: Ratomski
authors.forename: Edwina
authors.initials: E
authors.lastname: Kasprzycka
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Hassmann-Poznańska
mesh_terms: D000234:Adenoids / Q000276:immunology* / Q000601:surgery; D000293:Adolescent; D017252:CD3 Complex / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D008297:Male; D010034:Otitis Media with Effusion / Q000276:immunology* / Q000601:surgery; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D014068:Tonsillectomy
chemical_list: D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta