Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection.
Capillary zone electrophoresis (CZE) with fluorescence detection was applied to the simultaneous determination of histamine and polyamines including spermine, spermidine, diaminopropane, putrescine, cadaverine, diaminohexane with 4-fluor-7-nitro-2,1,3-benzoxadiazole (NBD-F) as the fluorescent derivatization reagent. The seven NBD-F labeled amines was separated within 200 s using 85 mM phosphate running buffer at pH 3.0. The concentration limits of these amines ranged from 5.1 x 10(-8) M for spermine to 2.1 x 10(-8) M for histamine. The relative standard deviations for migration time and peak height were less than 1.5% and 6.0%, respectively. The method was successfully applied to the analysis of biogenic amines in the lysate of tobacco mesophyll protoplasts, and spermidine and putrescine were detected in the lysate with satisfying recovery.
Zhang
Li-Yao
LY
Key Laboratory of MOE for Plant Development Biology, College of life Sciences, Wuhan University, Wuhan 430072, China.
Tang
Xing-Chun
XC
Sun
Meng-Xiang
MX
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006632:Histamine / Q000032:analysis*; D011073:Polyamines / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011073:Polyamines; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D006632:Histamine; D009327:4-Chloro-7-nitrobenzofurazan