Anti-inflammatory effects of phospholipid transfer protein (PLTP) deficiency in mice.
We previously reported that phospholipid transfer protein-deficient (PLTP KO) mice exhibit a lower rate of atherosclerosis. We proposed two possible mechanisms: a reduction in hepatic apoB secretion (Nat Med 7 (2001) 847) and induction of lipoprotein anti-oxidation activity (J Biol Chem 277 (2002) 31850). We now hypothesized that PLTP KO mice may exhibit an anti-inflammatory state per se. First, we found that PLTP KO mice have significantly lower IL-6 levels than wild type (WT) mice. Secondly, we found that IL-6 treatment increased plasma TNFalpha levels in WT mice, but not in PLTP KO mice. Thirdly, we used flow cytometric analyses to measure the mean fluorescence intensity of I-A(b), a MHC-class II molecule, on peripheral monocytes and found that IL-6 treatment significantly increased the I-A(b)-positive cell levels in WT mice, whereas no changes were observed in the cell levels in PLTP KO mice. The results of our experiments demonstrated an anti-inflammatory effect of PLTP deficiency as a further aspect of its proatherogenic potency.
Schlitt
Axel
A
Department of Anatomy and Cell Biology, State University of New York, Downstate Medical Center, Brooklyn, 11203, USA. axelschlitt@gmx.net
Liu
Jin
J
Yan
Daoguang
D
Mondragon-Escorpizo
Mary
M
Norin
Allen J
AJ
Jiang
Xian-Cheng
XC
D000818:Animals; D008076:Cholesterol, HDL / Q000097:blood; D000949:Histocompatibility Antigens Class II / Q000097:blood; D007249:Inflammation / Q000209:etiology*; D015850:Interleukin-6 / Q000097:blood / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D049294:Phospholipid Transfer Proteins / Q000172:deficiency* / Q000235:genetics; D010743:Phospholipids / Q000097:blood; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D008076:Cholesterol, HDL; D000949:Histocompatibility Antigens Class II; C474482:I-A(b) antigen, mouse; D015850:Interleukin-6; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha