title: Analysis of expressed sequence tags from oil palm (Elaeis guineensis).
abstract: This is the first report of a systematic study of genes expressed by means of expressed sequence tag (EST) analysis in oil palm, a species of the Arecales order, a phylogenetically key clade of monocotyledons that is not widely represented in the sequence databases. Five different cDNA libraries were generated from male and female inflorescences, shoot apices and zygotic embryos and unidirectional systematic sequencing was performed. A total of 2411 valid EST sequences were thus obtained. Cluster analysis enabled the identification of 209 groups of related sequences and 1874 singletons. Putative functions were assigned to 1252 of the set of 2083 non-redundant ESTs obtained. The EST database described here is a first step towards gene discovery and cDNA array-based expression analysis in oil palm.
authors.affiliation: IRD/CIRAD Oil Palm Laboratory, Centre IRD Montpellier, France. jouannic@mpl.ird.fr
authors.forename: Stefan
authors.initials: S
authors.lastname: Jouannic
authors.forename: Xavier
authors.initials: X
authors.lastname: Argout
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lechauve
authors.forename: Cécile
authors.initials: C
authors.lastname: Fizames
authors.forename: Alain
authors.initials: A
authors.lastname: Borgel
authors.forename: Fabienne
authors.initials: F
authors.lastname: Morcillo
authors.forename: Frédérique
authors.initials: F
authors.lastname: Aberlenc-Bertossi
authors.forename: Yves
authors.initials: Y
authors.lastname: Duval
authors.forename: James
authors.initials: J
authors.lastname: Tregear
mesh_terms: D028023:Arecaceae / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D018076:DNA, Complementary; D018744:DNA, Plant; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D015723:Gene Library; D008969:Molecular Sequence Data; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant