title: Genetic diversity of the internal transcribed spacers (ITS) and 5.8S rRNA genes among the clinical isolates of Candida parapsilosis in Brazil and Japan.
abstract: The internal transcribed spacer (ITS) region including 5.8S rDNA sequences of 58 isolates of Candida parapsilosis in Brazil and Japan was analyzed. Although most of the C. parapsilosis strains tested were confirmed to belong to three already reported genetically distinct groups (I, II and III) based on their ITS region sequences, 5 strains of the Brazilian isolates showed different sequences from those heretofore reported and suggested a presence of new genotype. For these strains of C. parapsilosis, we proposed a new genetic group (IV). The sequence similarities of this new group of IV to I, II and III were 87.4%, 94.7% and 87.3% in the ITS1 region, respectively. Genetic diversity in ITS regions of the remaining C. parapsilosis strains in Brazil and Japan was also discussed.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Soji
authors.initials: S
authors.lastname: Iida
authors.forename: Tamae
authors.initials: T
authors.lastname: Imai
authors.forename: Toyoko
authors.initials: T
authors.lastname: Oguri
authors.forename: Katsuko
authors.initials: K
authors.lastname: Okuzumi
authors.forename: Ademar
authors.initials: A
authors.lastname: Yamanaka
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Moretti-Branchini
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
mesh_terms: D001483:Base Sequence; D001938:Brazil; D002175:Candida / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D020459:Genes, rRNA; D014644:Genetic Variation; D006801:Humans; D007564:Japan; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*
chemical_list: D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S