title: New methods for the generation of carbohydrate arrays on glass slides and their evaluation.
abstract: Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precluding the necessity for the blocking of unreacted functional groups on the glass slide. The covalency and the concentration dependency of the sugar ligation to the glass slide were demonstrated; the reversibility and the selectivity of lectin-carbohydrate interactions were shown.
authors.affiliation: Fachbereich Chemie, Universität Konstanz, Fach M 725, 78457 Konstanz, Germany.
authors.forename: Moritz B
authors.initials: MB
authors.lastname: Biskup
authors.forename: Jan U
authors.initials: JU
authors.lastname: Müller
authors.forename: Ralf
authors.initials: R
authors.lastname: Weingart
authors.forename: Richard R
authors.initials: RR
authors.lastname: Schmidt
mesh_terms: D000577:Amides / Q000737:chemistry; D000586:Amination; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006027:Glycosides / Q000138:chemical synthesis; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008832:Microchemistry / Q000379:methods*
chemical_list: D000577:Amides; D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D006027:Glycosides; D037102:Lectins