title: [Differential expression of osteopontin and bax, bcl-2 by fluoride].
abstract: OBJECTIVE
  To study the differential expression of bax, bcl-2 and osteopontin by fluoride in the renal tubular cells in vitro.
  
  
  METHODS
  The renal tubular cells were cultured and exposed to sodium fluoride (NaF) in 1, 5, 7.5, 12.5 mg F-/L level. The transcription level of bax, bcl-2 and osteopontin were investigated by reverse transcription - polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The expression of bax mRNA in 7.5 and 12.5 mgF-/L groups (optical absorption ratio value was 2.37 +/- 0.18 and 2.64 +/- 0.19 respectively) was significantly increased (P < 0.01). On the contrary, the level of bcl-2 obviously decreased (5 mg F-/L group optical absorption ratio value, 0.80 +/- 0.22, P < 0.05; 7.5 mg F-/L group optical absorption ratio value 0.71 +/- 0.22, P < 0.01). The expression mRNA of osteopontin was significantly increased when cells were exposed to fluoride at 7.5 mg F-/L (optical absorption ratio value 2.01 +/- 0.40 P < 0.01), in that group the tubular cell apoptotic trend was obvious.
  
  
  CONCLUSIONS
  NaF might induce tubular cell apoptosis via activation of bax expression and bcl-2 suppression. Osteopontin might protect the tubule against apoptosis in a lower fluoride level, but the function should be decreased in higher fluoride level.
authors.affiliation: Department of Pathology, Institute of Frontier Medical Science, Jilin University, Changchun 130021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Jing-min
authors.initials: JM
authors.lastname: Zhang
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D020869:Gene Expression Profiling; D007684:Kidney Tubules / Q000166:cytology; D008297:Male; D053495:Osteopontin / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012969:Sodium Fluoride / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: D051017:Apoptosis Regulatory Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D053495:Osteopontin; D012969:Sodium Fluoride