[Effects of TCP/HA-coating titanium on the adhesion behavior of human gingival fibroblasts].
OBJECTIVE
To investigate effects of hydroxyapatite (HA) and porous tricalcium phosphate/hydroxyapatite (TCP/HA)-coating titanium on the adhesion behavior of human gingival fibroblasts (HGFs).


METHODS
Coatings of HA and duplex phases TCP/HA on titanium (Ti) were formed by ion beam assisted deposition (IBAD) method. Attachment, spreading, extracellular matrix (ECM) production, and focal adhesion plaque formation of HGFs were investigated on commercially pure (CP) titanium, HA-coated CP titanium and porous TCP/HA-coated CP titanium. After incubation of HGFs on these substrates, the number of attached cell, the area of cell spreading, immunostained ECM including fibronectin (FN) and type I collage, and vinculin (presenting the formation of focal adhesion plaque) were quantified by morphometric analysis using immunofluorescence microscope.


RESULTS
TCP/HA and HA coatings exhibited that the attached cell number and cell spreading area were greater than those of CP titanium (P < 0.05), and the formation of focal adhesion plaque was earlier than that of uncoated substrate (P < 0.05). The number of attached cell and the formation of type I collagen on TCP/HA were more than those on Ti and HA. After 24-hour incubation on TCP/HA surface, the number of attached cell was 198.1 +/- 27.7 and the fluorescent intensity of type I collagen was 154.10 +/- 31.56. While under the same condition, the corresponding numbers for the CP titanium were 125.1 +/- 29.9 and 132.63 +/- 35.26. The differences between the two groups were significant (P < 0.05).


CONCLUSIONS
In this study, the porous TCP/HA coating significantly facilitated the adherence of human gingival fibroblasts to Ti surface and could improve the biocompatibility of titanium.
Zhao
Bao-Hong
BH
Department of Prosthodontics, Hospital for Stomatology, China Medical University, Shenyang 110002, China.
Bai
Wei
W
Feng
Hai-Lan
HL
Cui
Fu-Zhai
FZ
D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D014025:Titanium / Q000737:chemistry*
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D014025:Titanium; C018392:tricalcium phosphate