title: Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells.
abstract: Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, covering about 80% of the estimated genome of this dimorphic, thermo-regulated fungus. The data provide a comprehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in mycelium and yeast cells was detected, which was confirmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about sequences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, 70910-900, Brasília, DF, Brazil. msueli@unb.br
authors.forename: Maria Sueli S
authors.initials: MS
authors.lastname: Felipe
authors.forename: Rosângela V
authors.initials: RV
authors.lastname: Andrade
authors.forename: Fabrício B M
authors.initials: FB
authors.lastname: Arraes
authors.forename: André M
authors.initials: AM
authors.lastname: Nicola
authors.forename: Andréa Q
authors.initials: AQ
authors.lastname: Maranhão
authors.forename: Fernando A G
authors.initials: FA
authors.lastname: Torres
authors.forename: Ildinete
authors.initials: I
authors.lastname: Silva-Pereira
authors.forename: Márcio J
authors.initials: MJ
authors.lastname: Poças-Fonseca
authors.forename: Elida G
authors.initials: EG
authors.lastname: Campos
authors.forename: Lídia M P
authors.initials: LM
authors.lastname: Moraes
authors.forename: Patrícia A
authors.initials: PA
authors.lastname: Andrade
authors.forename: Aldo H F P
authors.initials: AH
authors.lastname: Tavares
authors.forename: Simoneide S
authors.initials: SS
authors.lastname: Silva
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Kyaw
authors.forename: Diorge P
authors.initials: DP
authors.lastname: Souza
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
authors.forename: Rosália S A
authors.initials: RS
authors.lastname: Jesuíno
authors.forename: Edmar V
authors.initials: EV
authors.lastname: Andrade
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Parente
authors.forename: Gisele S
authors.initials: GS
authors.lastname: Oliveira
authors.forename: Mônica S
authors.initials: MS
authors.lastname: Barbosa
authors.forename: Natália F
authors.initials: NF
authors.lastname: Martins
authors.forename: Ana L
authors.initials: AL
authors.lastname: Fachin
authors.forename: Renato S
authors.initials: RS
authors.lastname: Cardoso
authors.forename: Geraldo A S
authors.initials: GA
authors.lastname: Passos
authors.forename: Nalvo F
authors.initials: NF
authors.lastname: Almeida
authors.forename: Maria Emília M T
authors.initials: ME
authors.lastname: Walter
authors.forename: Célia M A
authors.initials: CM
authors.lastname: Soares
authors.forename: Maria José A
authors.initials: MJ
authors.lastname: Carvalho
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Brígido
mesh_terms: D015152:Blotting, Northern; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D016681:Genome, Fungal; D020407:Internet; D008954:Models, Biological; D008969:Molecular Sequence Data; D025282:Mycelium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010228:Paracoccidioides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger