title: Determination of local anaesthetics and their impurities in pharmaceutical preparations using HPLC method with amperometric detection.
abstract: A method for the determination of local anaesthetics and their impurities--2,6-dimethylaniline and o-toluidine--by high-performance liquid chromatographic method with amperometric detection has been developed. The analysis was performed in an isocratic mode on a reversed phase Luna column 5 microm C-18 (100 mm x 4.6 mm). A mobile phase [0.01 mol l(-1) Tris buffer of pH 7.9:acetonitrile (45:55)] was selected for the separation and determination of studied anaesthetics and their impurities. Chromatograms were recorded for 500 s by means of an amperometric detector at a potential of +1.0 V of the glassy carbon electrode versus the reference electrode Ag/AgCl. The proposed liquid chromatographic method was successfully applied to the analysis of commercially available pharmaceutical preparations. The limit of the detection for 2,6-dimethylaniline and o-toluidine was 0.8 ng ml(-1). The limit of quantitation, considering a signal to noise ratio was 1.5 ng ml(-1). The method developed in this study is sensitive and selective and can be applied to routine studies of pharmaceuticals in the form of cream and injection.
authors.affiliation: Department of Pharmaceutical Chemistry, National Institute of Public Health, 30/34 Chełmska Str., 00-725 Warsaw, Poland. fijalek@il.waw.pl
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Fijałek
authors.forename: Emil
authors.initials: E
authors.lastname: Baczyński
authors.forename: Agata
authors.initials: A
authors.lastname: Piwońska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Warowna-Grześkiewicz
mesh_terms: D000779:Anesthetics, Local / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination; D004563:Electrochemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D000779:Anesthetics, Local; D004364:Pharmaceutical Preparations