Sandwich immunoassays for the determination of peanut and hazelnut traces in foods.
People suffering from food allergies are dependent on accurate food labeling, as an avoidance diet is the only effective countermeasure. Even a small amount of allergenic protein can trigger severe reactions in highly sensitized patients. Therefore, sensitive and reliable tests are needed to detect potential cross-contamination. In this paper two fast sandwich immunoassays are described for the determination of peanut (Arachis hypogaea) and hazelnut (Corylus avellana) traces in complex food matrices. Mouse monoclonal antibodies were used as capture antibodies, and labeled rabbit polyclonal antibodies were used as detection antibodies in both assays. The assay time was 30 min in total, and cross-reactivities against a variety of fruits and seeds were found to be in the low 10(-4)% (ppm) level or in some cases not detectable. The recoveries in all tested food matrices ranged from 86 to 127%, and the limits of detection were in the range of 0.2-1.2 mg/kg (ppm) in food for both peanut and hazelnut, respectively.
Kiening
Martin
M
Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München, Germany.
Niessner
Reinhard
R
Drs
Elisabeth
E
Baumgartner
Sabine
S
Krska
Rudolf
R
Bremer
Monique
M
Tomkies
Victoria
V
Reece
Paul
P
Danks
Chris
C
Immer
Ulrike
U
Weller
Michael G
MG
D000485:Allergens / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D010367:Arachis / Q000737:chemistry / Q000276:immunology*; D031211:Corylus / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D051379:Mice; D010940:Plant Proteins / Q000032:analysis; D012680:Sensitivity and Specificity
D000485:Allergens; D000911:Antibodies, Monoclonal; D010940:Plant Proteins