title: [Detection of the metabolites of dehydroepiandrosterone in urine with gas chromatography-combustion-isotope ratio mass spectrometry].
abstract: OBJECTIVE
  To establish a method to determine the isotope ratios of 13C to 12C of dehydroepiandrosterone and its metabolites in urine, for detecting the source of dehydroepiandrosterone or its metabolites.
  
  
  METHODS
  Preliminary separation of endogenous anabolic androgenic steroids could be achieved using solid phase extraction, enzymolysis and thin layer chromatography. The source of dehydroepiandrosterone and other endogenous anabolic androgenic steroids could be detected by their delta values with gas chromat ography-combustion-isotope ratio mass spectrometry.
  
  
  RESULTS
  The 5 values of some metabolites of dehydroepiandrosterone reduced after the administration of dehydroepiandrosterone preparation. In these cases the data indicated that exogenous anabolic androgenic steroids were administrated.
  
  
  CONCLUSIONS
  The source of dehydroepiandrosterone or its metabolites in urine could be detected by measuring their delta values with this method.
authors.affiliation: National Research Institute of Sports Medicine, Beijing 100029, China. jzhw@yahoo.com
authors.forename: Jing-zhu
authors.initials: JZ
authors.lastname: Wang
authors.forename: Mou-tian
authors.initials: MT
authors.lastname: Wu
authors.forename: Yi-nong
authors.initials: YN
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Yang
mesh_terms: D000328:Adult; D015113:Androstane-3,17-diol / Q000652:urine; D000738:Androsterone / Q000652:urine*; D002855:Chromatography, Thin Layer / Q000379:methods; D003687:Dehydroepiandrosterone / Q000378:metabolism*; D004300:Doping in Sports; D005043:Etiocholanolone / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D011279:Pregnanetriol / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods
chemical_list: D015113:Androstane-3,17-diol; D011279:Pregnanetriol; D003687:Dehydroepiandrosterone; D005043:Etiocholanolone; D000738:Androsterone