Simultaneous determination of anabolic steroids and synthetic hormones in meat by freezing-lipid filtration, solid-phase extraction and gas chromatography-mass spectrometry.
Estradiol, testosterone, progesterone, zeranol and diethylstilbestrol including estradiol metabolites were determined simultaneously in meat. Extraction of growth hormones was carried out by ultasonication using a methanol-water mixture. The growth hormones in the meat extract can be effectively separated from lipids by freezing-lipid filtration, followed by C8-solid phase extraction (SPE). During freezing-lipid filtration, about 90% of lipids are removed without any significant loss of growth hormones. For further clean-up, silica- and aminopropyl-SPE were used. To enhance detection sensitivity, the growth hormones are derivatized with trimethylsilyl reagents. Quantitation using isotope-labelled internal standards was performed by gas chromatography-mass spectrometry in the selected ion monitoring mode. The method detection limits were 0.1-0.4 microg/kg for all growth hormones. Overall recoveries of synthetic and natural growth hormones were 68-106% with coefficients of variation of 5-16% for the complete procedure.
Seo
Jungju
J
Hazardous Substance Research Team, Korea Basic Science Institute, 126-16 Anam-Dong, Sungbuk-Ku, 136-701 Seoul, South Korea.
Kim
Hye-Young
HY
Chung
Bong Chul
BC
Hong
Jongki
J
D045930:Anabolic Agents / Q000032:analysis*; D005374:Filtration / Q000379:methods*; D005615:Freezing; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006728:Hormones / Q000032:analysis*; D008055:Lipids / Q000302:isolation & purification*; D008461:Meat Products / Q000032:analysis*; D013256:Steroids / Q000032:analysis*
D045930:Anabolic Agents; D006728:Hormones; D008055:Lipids; D013256:Steroids