title: Cell microarrays on photochemically modified polytetrafluoroethylene.
abstract: We studied the adhesion, proliferation, and viability of human umbilical vein endothelial cells (HUVEC) and human embryonic kidney cells (HEK) on modified spots at polytetrafluoroethylene (PTFE) surfaces. The viability of the cells was assessed using an aqueous non-radioactive cell proliferation assay. Round spots with a diameter of 100 microm were modified by exposure to the ultraviolet (UV) light of a Xe(2)(*)-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere employing a contact mask. The spots were arranged in a quadratic pattern with 300 microm center-to-center spot distances. With optimized degree of modification, the cells adhered to the modified spots with a high degree of selectivity (70-90%). The adhered cells on the spots proliferated. This resulted in a significant increase in the number of adhering HUVECS or HEK cells after seeding and in the formation of confluent cell clusters after 3-4 days. With higher start seeding density, these clusters were not only confined to the modified spots but extended several micrometer to the neighborhood. The high potential of the cell microarrays for gene analysis in living cells was demonstrated with HEK cells transfected by yellow fluorescent protein (YFP).
authors.affiliation: Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague Czech Republic.
authors.forename: Regina
authors.initials: R
authors.lastname: Mikulikova
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Moritz
authors.forename: Thomas
authors.initials: T
authors.lastname: Gumpenberger
authors.forename: Michael
authors.initials: M
authors.lastname: Olbrich
authors.forename: Christoph
authors.initials: C
authors.lastname: Romanin
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
authors.forename: Vaclav
authors.initials: V
authors.lastname: Svorcik
authors.forename: Johannes
authors.initials: J
authors.lastname: Heitz
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000528:radiation effects; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005169:Factor VIII / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D008027:Light; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D010777:Photochemistry / Q000379:methods*; D011138:Polytetrafluoroethylene / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D011994:Recombinant Proteins / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011994:Recombinant Proteins; D005169:Factor VIII; D011138:Polytetrafluoroethylene