title: [A study on primary culture in vitro of twenty-one laryngeal squamous carcinoma tissues].
abstract: OBJECTIVE
  To study the different influent on the growth rate of laryngeal carcinoma cell culture in vitro between collagenase type I and trypsin and to provide the experiment base for laryngeal carcinoma cell culture.
  
  
  METHODS
  Applying the technique of primary tissue culture in vitro, twenty-one specimens which were identified pathologically as laryngeal squamous cell carcinoma were cultured. The relation ship letwech the cell growth rate and donor age, cultured methods was observed. The influent factors of primary culture, such as serum of low concentration were analyzed.
  
  
  RESULTS
  Cell growth rate were 66% (6/9) in the group aged under 60.58% in the group above 60. The cell growth rate were 58% in collagenase type I and 13% in trypsin. Serum of low concentration can restrain fibroblast.
  
  
  CONCLUSIONS
  The cell growth of laryngeal squamous cell carcinoma in vitro could be correlated with the culture methods, fibroblast outgrowth and microbiotic contamination are important factors in primary cell culture.
authors.affiliation: Department of Otorhinolaryngology, First Hospital of Shanxin Medical University, Taiyuan 030001, China. wangjinyuan1967@163.com
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Wang
authors.forename: Binquan
authors.initials: B
authors.lastname: Wang
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D017364:Collagenases / Q000378:metabolism; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D014357:Trypsin; D017364:Collagenases