title: Cell viability of chitosan-containing semi-interpenetrated hydrogels based on PCL-PEG-PCL diacrylate macromer.
abstract: Chitosan-modified biodegradable hydrogels were prepared by UV irradiation of solutions in mild aqueous acidic media of poly(caprolactone)-co-poly(ethylene glycol)-co-poly(caprolactone) diacrylate (PCL-PEG-PCL-DA) and chitosan. Hydrogels obtained were characterized using FT-IR, DSC, TGA and XPS. FT-IR, TGA and DSC revealed the semi-interpenetrating polymer network structure formed in the hydrogel. Though the water swelling degree of these chitosan-modified hydrogels was substantial in the range of 322-539%, it was found that fibroblasts could still attach, spread and grow on them; this is in contrast to the commonly investigated PEG-diacrylate hydrogel. The MTT assay demonstrated that cells could grow better on 3 or 6% chitosan-modified hydrogel than unmodified PCL-PEG-PCL-DA hydrogels or low-content (1%) chitosan-modified PCL-PEG-PCL-DA hydrogel. Increased chitosan content resulted in increased cell interaction and also decreased water swelling, both of which results in increased cell attachment and spread.
authors.affiliation: Biological and Chemical Processing Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Republic of Singapore.
authors.forename: Ai Ping
authors.initials: AP
authors.lastname: Zhu
authors.forename: Mary B
authors.initials: MB
authors.lastname: Chan-Park
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004396:Coloring Agents / Q000494:pharmacology; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000378:metabolism; D006358:Hot Temperature; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D008027:Light; D051379:Mice; D041681:NIH 3T3 Cells; D009584:Nitrogen / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013778:Tetrazolium Salts / Q000737:chemistry / Q000494:pharmacology; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D014466:Ultraviolet Rays; D014867:Water
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D004396:Coloring Agents; D020100:Hydrogels; D011091:Polyesters; D011108:Polymers; D011137:Polystyrenes; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; C016240:polycaprolactone; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D048271:Chitosan; C022616:thiazolyl blue; D009584:Nitrogen