title: Microarray and proteomics analyses of human intestinal epithelial cells treated with the Aeromonas hydrophila cytotoxic enterotoxin.
abstract: We performed microarray analyses on RNA from human intestinal epithelial (HT-29) cells treated with the cytotoxic enterotoxin (Act) of Aeromonas hydrophila to examine global cellular transcriptional responses. Based on three independent experiments, Act upregulated the expression of 34 genes involved in cell growth, adhesion, signaling, immune responses (including interleukin-8 [IL-8] production), and apoptosis. We verified the upregulation of 14 genes by real-time reverse transcriptase-PCR and confirmed Act-induced production of IL-8 by enzyme-linked immunosorbent assay on supernatants from nonpolarized and polarized HT-29 cells. Maximal production of IL-8 in response to Act required the presence of intracellular calcium, since chelation of calcium with BAPTA-AM significantly reduced Act-induced IL-8 production in HT-29 cells. We also examined activation of mitogen-activated protein kinases and, as demonstrated by Western blot analysis of apical side-treated polarized HT-29 cells, Act induced phosphorylation of p38, c-Jun NH(2)-terminal kinase, and extracellular signal-regulated kinase 1/2. In addition, KinetWorks proteomics screening of whole-cell lysates revealed Act-induced phosphorylation of cyclic AMP-response element binding protein (CREB), c-Jun, adducin, protein kinase C, and signal transducer and activator of transcription 3 (STAT3) and decreased phosphorylation of protein kinase Balpha, v-raf-1 murine leukemia viral oncogene homolog 1 (i.e., Raf1), and STAT1. We verified activation of CREB and activator protein 1 in polarized cells by gel shift assay. This is the first description of human intestinal epithelial cell transcriptional alterations, phosphorylation or activation of signaling molecules, cytokine production, and calcium mobilization in response to this toxin.
authors.affiliation: Department of Microbiology and Immunology, Medical Research Building, 301 University Blvd., University of Texas Medical Branch, Galveston, TX 77555-1070, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Galindo
authors.forename: A A
authors.initials: AA
authors.lastname: Fadl
authors.forename: Jian
authors.initials: J
authors.lastname: Sha
authors.forename: L
authors.initials: L
authors.lastname: Pillai
authors.forename: C
authors.initials: C
authors.lastname: Gutierrez
authors.forename: A K
authors.initials: AK
authors.lastname: Chopra
mesh_terms: D016980:Aeromonas hydrophila / Q000378:metabolism / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D004768:Enterotoxins / Q000378:metabolism / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D004768:Enterotoxins; D011506:Proteins; C082174:cytolytic enterotoxin protein, Aeromonas