Determination of poliovirus-specific IgA in saliva by ELISA tests.
This study describes three ELISA methods for detection of immunoglobulin A (IgA) specific to three types of Sabin strains of poliovirus in saliva taken from 70 children aged 6-7 years vaccinated with a full course of oral poliovirus vaccine (OPV). Of the three ELISA methods (conventional IgA ELISA and two new methods described in this communication, the alpha-capture ELISA and Inhibition ELISA), alpha-capture ELISA demonstrated the highest sensitivity, with all saliva samples testing positive for Sabin poliovirus strains specific IgA antibodies of 1-3 types. Of 62 available alpha-capture ELISA positive saliva samples, all were also positive by the inhibition ELISA, and a significant correlation was found between the results. Fifty-two available saliva samples were screened by the three ELISA tests with positive results, and a significant correlation was found between the alpha-capture ELISA and the IgA ELISA; the correlation between the IgA ELISA and inhibition ELISA was not significant. The results of this study suggest that determination of Sabin poliovirus-specific IgA in human saliva by the ELISA techniques (especially by the novel alpha-capture ELISA) can be used reliably for evaluation of mucosal immunity in large groups of people immunized with poliovirus vaccines and for epidemiological studies.
Ivanov
Alexander
A
Center for Biologics Evaluation and Research, Food and Drug Administration, HFM-470, NLRC/B-121, 1401 Rockville Pike, Rockville, MD 20852, USA. ivanov@cber.fda.gov
Dragunsky
Eugenia
E
Ivanova
Olga
O
Rezapkin
Gennady
G
Potapova
Svetlana
S
Chumakov
Konstantin
K
D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D017955:Poliovirus / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral / Q000276:immunology*; D012463:Saliva / Q000276:immunology*
D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory; D011055:Poliovirus Vaccine, Oral