title: The effect of chronic calcium treatment on thyroid C cells in ovariectomized rats.
abstract: The purpose of this study was to investigate the influence of chronic calcium treatment on the structure and function of thyroid C cells in ovariectomzed adult female rats. Eighteen 3-month-old, female Wistar rats were divided into three groups. The first group was used as the sham-operated control, and the other two were surgically ovariectomized (Ovx). One month after gonadectomy, one group of Ovx rats was injected with 28.55 mg Ca-glucoheptonate (Ca)/kg b.w., while the other two groups were chronically treated with vehicle alone (Ovx and sham control). Two months after surgery, the animals were killed. In the thyroid C cells, calcitonin (CT) was localized with the peroxidase-antiperoxidase method. Stereology was used to evaluate morphometric changes in the volume of C cells, their nuclei and relative volume density. The number of C cells per unit area was calculated. Serum CT content was determined by radioimmunoassay. After chronic Ca treatment C cells were numerous with darker cytoplasm than in C cells of sham-operated control animals, but more degranulated than the corresponding cells of Ovx rats. Their volume was significantly decreased by 14% (p < 0.05), while the number was increased by 47% (p < 0.05) in comparison with corresponding controls. Serum CT concentration was decreased by 27% (n.s.) in comparison to sham-operated control. Calcium treatment of Ovx rats led to a 32% increase of serum CT concentration in relation to untreated Ovx animals. These results suggest that chronic Ca treatment of Ovx female rats positively affected CT release from thyroid C cells, without any significant changes in morphometric parameters.
authors.affiliation: Institute for Biological Research, Despota Stefana 142, 11060 Belgrade, Serbia and Montenegro. brankof@ibiss.bg.ac.yu
authors.forename: B
authors.initials: B
authors.lastname: Filipović
authors.forename: B Sosić
authors.initials: BS
authors.lastname: Jurjević
authors.forename: M Manojlović
authors.initials: MM
authors.lastname: Stojanoski
authors.forename: N
authors.initials: N
authors.lastname: Nestorović
authors.forename: V
authors.initials: V
authors.lastname: Milosević
authors.forename: M
authors.initials: M
authors.lastname: Sekulić
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000096:biosynthesis / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D004967:Estrogens / Q000502:physiology*; D005260:Female; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004967:Estrogens; D002116:Calcitonin; D002118:Calcium