Pharmacokinetics of mangiferin in rat plasma after oral administration of a single dose of suanzaoren decoction.
OBJECTIVE
To study the pharmacokinetics of mangiferin in rats after oral administration of a single dose of Suanzaoren decoction.


METHODS
An HPLC method was established using puerain as internal standard. Plasma samples were deproteinized with acetonitrile-acetic acid (9:1), followed by evaporation of the acetonitrile to dryness. The resultant residue was then dissolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (200 mm x 4.6 mm ID, 5 microm) column at room temperature. The mobile phase consisted of acetonitrile-water (12:88) with 1% acetic acid and 1% tetrahydrofuran at a flow rate of 0.7 mL x min(-1). The UV detection wavelength was set at 320 nm.


RESULTS
The calibration curve was shown to be linear over the range from 0.536 to 26.8 microg x mL(-1) (r2 > or = 0.995). Mean recovery was determined as 92.7%. Within-day and between-day precisions were less than 9. 1% RSD. The limit of quantitation (LOQ) was 0.536 microg x mL(-1). The maximum plasma concentration (Cmax), the time to reach peak concentration (Tmax) and the apparent elimination half-life (T1/2) were (10.5 +/- 2.2) microg x mL(-1), (5.8 +/- 0.4) h and (5.0 +/- 0.3) h, respectively.


CONCLUSIONS
The validated HPLC method developed has been applied to take a limited view of pharmacokinetics profile of mangiferin in rat plasma after having orally taken a single dose of Suanzaoren decoction.
Li
Yu-juan
YJ
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Bi
Kai-shun
KS
D000284:Administration, Oral; D031403:Anemarrhena / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D044004:Xanthones / Q000097:blood / Q000493:pharmacokinetics*; D031957:Ziziphus / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044004:Xanthones; C013592:mangiferin