Thermodynamic analysis of interactions between N-linked sugar chains and F-box protein Fbs1.
Fbs1 is a recently discovered F-box protein that was proposed to recognize high-mannose-type asparagine-linked glycoprotein sugar chains. To reveal the specificity of Fbs1, Manalpha1-->6Manbeta1-->4GlcNAc(2), Manalpha1-->3Manbeta1-->4GlcNAc(2), and Manalpha1-->3(Manalpha1--> 6)Manbeta1-->4GlcNAc(2) were synthesized and their affinities for Fbs1 were evaluated in comparison with previously synthesized Man(9)GlcNAc(2) and Man(8)GlcNAc(2). These analyses revealed that Man(3)GlcNAc(2) had the strongest affinity and the chitobiose and alpha1-->6 linked Man residue are necessary for Fbs1 to recognize a sugar.
Hagihara
Shinya
S
RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Totani
Kiichiro
K
Matsuo
Ichiro
I
Ito
Yukishige
Y
D002240:Carbohydrate Sequence; D044782:F-Box Motifs; D044783:F-Box Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011485:Protein Binding; D013816:Thermodynamics
D044783:F-Box Proteins; D009844:Oligosaccharides