Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity.
For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.
Saitoh
Satoshi
S
Toyota Biotechnology & Afforestation Laboratory, Aichi 480-1192, Japan.
Ishida
Nobuhiro
N
Onishi
Toru
T
Tokuhiro
Kenro
K
Nagamori
Eiji
E
Kitamoto
Katsuhiko
K
Takahashi
Haruo
H
D005285:Fermentation; D005818:Genetic Engineering; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D019344:Lactic Acid / Q000096:biosynthesis*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014920:Wine
D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase