[Experiment of genes expression of chemokines and their receptors in allergic rhinitis with gene chip].
OBJECTIVE
To analyze the role of chemokines and their receptors in the mechanism of allergic rhinitis (AR) by observing the expression of genes of chemokines and their receptors in nasal mucosa of AR through gene chip.


METHODS
The total RNAs were isolated from nasal mucosa of AR and purified to mRNAs, then reversely transcribed to cDNAs and incorporated with fluorescent-labled CY5-dUPT for probes preparion. CY3-dUTP probes prepared with normal nasal mucosa of normal for control. The chip contains cDNAs of chemokines and their receptors were used to hybridized with probes then screened with computer to study the expression of genes based on different density of fluorescent.


RESULTS
The chemokines of CCL11 (eotaxin-1), CCL24 (eotaxin-2), CCL7 (MCP-3), CCL13 (MCP-4), RANTES (CCL5) and receptors of CCR2, CCR3, CCR4, CCR5 were differential expressed on four samples, and most of the chemokines and their receptors has tendency regulation on T help 2 (Th2) lymphocytes and involved in the prodeeds such as inducement of allergic reaction,accumulation of inflammacytes and degranulation of sensitized cells.


CONCLUSIONS
A disorder exists in T helper immune system with AR. The chemokines and their receptors that polarized with Th2 lymphocytes perhaps play important roles in AR pathogenesis, and it represents a new approach to AR immunotherapy.
Yu
Shaoqing
S
Department of Otorhinolaryngology, Changhai Hospital, the Second Military University, Shanghai, 200433, China.
Zhang
Ruxin
R
Liu
Guojun
G
Wen
Wu
W
Shen
Xiaohua
X
D000328:Adult; D019742:Chemokines, CC / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D019707:Receptors, Chemokine / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology / Q000235:genetics*
D019742:Chemokines, CC; D019707:Receptors, Chemokine