title: Escherichia coli K1 RS218 interacts with human brain microvascular endothelial cells via type 1 fimbria bacteria in the fimbriated state.
abstract: Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.
authors.affiliation: Division of Pediatric Infectious Diseases, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA.
authors.forename: Ching-Hao
authors.initials: CH
authors.lastname: Teng
authors.forename: Mian
authors.initials: M
authors.lastname: Cai
authors.forename: Sooan
authors.initials: S
authors.lastname: Shin
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Kee-Jun
authors.initials: KJ
authors.lastname: Kim
authors.forename: Naveed Ahmed
authors.initials: NA
authors.lastname: Khan
authors.forename: Francescopaolo
authors.initials: F
authors.lastname: Di Cello
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Kim
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D001921:Brain / Q000098:blood supply*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000382:microbiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D017353:Gene Deletion; D006801:Humans; D007231:Infant, Newborn; D008833:Microcirculation / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D011817:Rabbits
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; C058769:fimH protein, E coli; D036781:Fimbriae Proteins