Functional dissociation of the basolateral transcytotic compartment from the apical phago-lysosomal compartment in human osteoclasts.
Tartrate-resistant acid phosphatase (TRAP) is essential for elimination of Staphylococcus aureus, the main infectious agent responsible for osteomyelitis. This in vitro study investigated uptake and processing of fluorescence-labeled S. aureus by human osteoclasts and dendritic cells. The cells were stained for TRAP and the acidic compartment using a fluorescence-based protocol. In dendritic cells, TRAP and bacteria were colocalized. In osteoclasts, there was no colocalization of bacteria, TRAP, or the acidic compartment, indicating that there are three distinct vesicular compartments: the apical phago-lysosomal compartment, the basal secretory compartment, and the basolateral transcytotic compartment. Dissociation of the TRAP-containing transcytotic vesicles from the apical phago-lysosomal compartment may restrain osteoclasts from eliminating S. aureus.
Meagher
James
J
School of Anatomy and Human Biology, The University of Western Australia, Crawley WA 6009, Australia.
Zellweger
René
R
Filgueira
Luis
L
D000135:Acid Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure; D004705:Endocytosis; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008247:Lysosomes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010010:Osteoclasts / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D010586:Phagocytes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D013211:Staphylococcus aureus / Q000502:physiology*; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase