[The research on the quenching of fluorescence spectra of TOP I by iodine anion].
Comparing the fluorescence spectra of tobacco peroxidase I (TOP I) solution and the solutions titrated by iodine anions, the number of binding locus and the binding constant of iodine anion to TOP I were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOP I molecule were discussed.
Xia
Bing-le
BL
Department of Chemistry, USTC, Hefei 230026, China.
Peng
Dun-geng
DG
Li
Min-li
ML
Xiao
Hong
H
Wang
Kai-bao
KB
D000465:Algorithms; D000838:Anions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007455:Iodine / Q000737:chemistry; D007700:Kinetics; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012107:Research Design; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology*
D000838:Anions; D010940:Plant Proteins; D007455:Iodine; D009195:Peroxidase