Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography.
Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of quercetin-3-O-L-rhamnoside from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase-system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5 (v/v/v). In a single operation, 41.9mg of quercetin-3-O-L-rhamnoside was obtained from 366mg of the crude extract. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of quercetin-3-O-L-rhamnoside was over 96%. Its structure was identified by MS, 1H NMR and 13C NMR. Quercetin-3-O-L-rhamnoside was obtained from this plant for the first time.
Ma
Xiaofeng
X
Department of Biology, Graduate School of Chinese Academy of Sciences, Beijing 10049, China.
Tian
Weixi
W
Wu
Linhuan
L
Cao
Xueli
X
Ito
Yoichiro
Y
D031002:Acer / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D011794:Quercetin / Q000302:isolation & purification*
D011794:Quercetin