title: Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
abstract: A role for endocytosis and exocytosis in cell migration has been proposed but not yet demonstrated. Here, we show that cellubrevin (Cb), an early endosomal v-SNARE, mediates trafficking in the lamellipod of migrating epithelial cells and partially colocalizes with markers of focal contacts. Expression of tetanus neurotoxin, which selectively cleaves Cb, significantly reduced the speed of migrating epithelial cells. Furthermore, expression of tetanus neurotoxin enhanced the adhesion of epithelial cells to collagen, laminin, fibronectin, and E-cadherin; altered spreading on collagen; and impaired the recycling of beta1 integrins. These results suggest that Cb-dependent membrane trafficking participates in cell motility through the regulation of cell adhesion.
authors.affiliation: Membrane Traffic in Neuronal and Epithelial Morphogenesis, Institut National de la Santé et de la Recherche Médicale Avenir Team, Centre National de la Recherche Scientifique, Universités Paris 6 et 7, 75005 Paris, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Proux-Gillardeaux
authors.forename: Julie
authors.initials: J
authors.lastname: Gavard
authors.forename: Theano
authors.initials: T
authors.lastname: Irinopoulou
authors.forename: René-Marc
authors.initials: RM
authors.lastname: Mège
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D039641:Integrin beta Chains / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D013744:Tetanus Toxin / Q000378:metabolism*; D014162:Transfection; D051040:Vesicle-Associated Membrane Protein 3; D014741:Video Recording
chemical_list: D015820:Cadherins; D005353:Fibronectins; D039641:Integrin beta Chains; D007797:Laminin; D008565:Membrane Proteins; D013744:Tetanus Toxin; D051040:Vesicle-Associated Membrane Protein 3; C035979:tetanospasmin; D003094:Collagen; D008666:Metalloendopeptidases