title: Ovarian follicular development stimulated by leuprorelin acetate plus human menopausal gonadotropin in chimpanzees.
abstract: We attempted ovarian stimulation using gonadotropins in 14 chimpanzees. Subjects were given a single administration of leuprorelin acetate, followed by repeated administration of human menopausal gonadotropin (hMG) for 16-21 days. During the dosing period, the ovarian follicle diameter and count were measured by transvaginal ultrasonography. The hormone administration induced the development of multiple follicles, and multiple oocytes were subsequently retrieved. However, the follicle count was decreased, suggesting atresia, in some subjects. Statistically, the final follicle diameter was dependent on the dosing duration and the hMG dose in the late stage, while the maximum follicle count during hMG administration was dependent on age and the hMG dose in the early stage. Five subjects showed mild ovarian hyperstimulation syndrome (OHSS)-like symptoms with a high serum estradiol (E2) concentration. These results suggest that leuprorelin acetate plus hMG administration successfully stimulates the development of multiple ovarian follicles for oocyte retrieval and that the serum E2 concentration is predictive of OHSS-like symptoms in chimpanzees.
authors.affiliation: Kumamoto Primates Research Park, Sanwa Kagaku Kenkyusho Co., Ltd, Kumamoto, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Kohji
authors.initials: K
authors.lastname: Shimoda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Honda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okamura
authors.forename: Yukie
authors.initials: Y
authors.lastname: Ide
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Nakashima
authors.forename: Naomi
authors.initials: N
authors.lastname: Nakagata
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Torii
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hayasaka
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005496:Follicular Atresia; D006801:Humans; D007700:Kinetics; D016729:Leuprolide / Q000008:administration & dosage*; D008596:Menotropins / Q000008:administration & dosage*; D008992:Monkey Diseases / Q000097:blood; D009865:Oocytes / Q000502:physiology; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000254:growth & development*; D016471:Ovarian Hyperstimulation Syndrome / Q000097:blood / Q000662:veterinary; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D002679:Pan troglodytes; D011374:Progesterone / Q000097:blood; D013480:Superovulation; D014463:Ultrasonography
chemical_list: D011374:Progesterone; D004958:Estradiol; D008596:Menotropins; D016729:Leuprolide