[Differentiation of bone marrow derived Thy-1+ beta2M- cells into liver cells in AA induced liver injury micro-environment].
OBJECTIVE
To investigate the differentiation of bone marrow derived Thy-1+ beta2M- cells (BDTCs) into liver cells in allyl alcohol (AA) induced liver injury micro-environment.


METHODS
BDTCs of male F344 rats were isolated by two-step magnetic separation system (MACS) technique, and infused intraportally into female recipients after labeling with PKH26. Thirty recipients were divided randomly into 3 groups: (1) AA-injured liver + BDTCs infusion, (2) normal liver + BDTCs infusion and (3) AA-injured liver + NS infusion (control). Blood biochemical examination, fluorescence labeled cellular localization, Y-chromosome sry gene in-situ hybridization and immunohistochemistry were carried out to evaluate BDTCs distribution, differentiation and proliferation in recipients's livers after different intervals.


RESULTS
Fluoromicroscopy and in situ hybridization suggested that BDTCs of donors were interspersed in pieces and cords among the necro-periportals induced by AA; immunohistochemistry indicated that those implanted cells expressed OV-6, AFP, CK19 and albumin successively, while positive cells were hardly seen in the normal liver + BDTCs infusion group. Compared with the controls, the blood biochemical restitution was more rapid in group (1), (9.8 d +/- 3.1 d vs. 13.7 d +/- 4.2 d).


CONCLUSIONS
The injury micro-environment induced by AA facilitates BDTCs integration with hepatic cell plates and differentiation into mature liver cells. BDTCs differentiation into liver cells might accelerate endogenous liver cell regeneration and reparation.
Wang
Yun-Fang
YF
Department of Stem Cell Biology, Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Nan
Xue
X
Yu
Cheng-Ze
CZ
Li
Yan-Hua
YH
Zhang
Rui
R
Guan
Li-Dong
LD
Yue
Wen
W
Pei
Yue-Tao
YT
D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology*; D022781:Hepatocytes / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D020005:Propanols; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344
D020005:Propanols; C006463:allyl alcohol