[Application of an alternative method to counting in Neubauer chamber to determine Trichomonas vaginalis concentrations].
It was demonstrated that there is a relation of cell concentration with the parasite reading in an ELISA reader. It was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. Likewise, it was confirmed that absorbance significantly increased (p < 0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of the various cell concentrations, it was proved that the optimum volume was 150 ml, a r2 = 0,9986 was reached, and the coefficient of correction(p < 0,001) and the gradient(p < 0,001)were highly significant. In the 5 x 10(4) to 1.5 x 10(7) cell/mL interval, there was an average variation coefficient of 1.75% (0.25-3.17%). Under these conditions, the quantification limit was 5,14 x 10(4) cell/mL. Finally, it was shown that the correlation of counting in Neubauer chamber and optical density was significant.
Marcel
A M
AM
Centro de Bioactivos Químicos, Universidad Central de Las Villas, Carretera a Camajuaní Km 51/2, Santa Clara, Villa Clara, Cuba. cbq@cbq.vcl.sld.cu
Rojas
L
L
Rodríguez
S S
SS
López
Y
Y
Ramos
I S
IS
D000818:Animals; D019411:Clinical Laboratory Techniques; D010274:Parasitology / Q000379:methods; D013997:Time Factors; D014246:Trichomonas vaginalis / Q000302:isolation & purification*