title: Studies on palauan medicinal herbs. II. Activation of mouse macrophages RAW 264.7 by Ongael, leaves of Phaleria cumingii (Meisn.) F. Vill. and its acylglucosylsterols.
abstract: The extract of Ongael [leaves of Phaleria cumingii (MEISN.) F. VILL.], a Palauan medicinal herb, enhanced an in vitro phagocytic activity of mouse macrophages RAW 264.7 cells (RAW 264.7). Activity-guided fractionation of the Ongael extract by the in vitro phagocytosis assay using RAW 264.7 led to the isolation of a mixture of acylglucosylsterols (1) as an active constituent along with other inactive constituents, tetracosanol and mangiferin. On the basis of chemical modifications and spectral analyses, the compound 1 was deduced to be a mixture of the known 3-O-(6-O-acyl-beta-D-glucosyl)-beta-sitosterols, the acyl moiety being mainly palmitoyl (57%), oleoyl (12%) and alpha-linolenoyl (12%) with small amount of stearoyl (7%) and linoleoyl (4%).
authors.affiliation: School of Pharmaceutical Sciences, Kinki University, Osaka 577-0818, Japan. matsuda@phar.kindai.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Tokunaga
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Naruto
authors.forename: Hideki
authors.initials: H
authors.lastname: Yagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Masuko
authors.forename: Michinori
authors.initials: M
authors.lastname: Kubo
mesh_terms: D000818:Animals; D002460:Cell Line; D008262:Macrophage Activation / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D031783:Phalaris; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D010840:Phytosterols; D010936:Plant Extracts