title: Characterization and expression analysis of the chicken interleukin-11 receptor alpha chain.
abstract: Interleukin-11 (IL-11) is a multifunctional cytokine involved in various pathways in blood cells, their precursors and many other cell types in vitro and in vivo. The effects of IL-11 are largely mediated by the IL-11 receptor alpha-chain (IL-11Ralpha). In this study, a putative cDNA sequence encoding the 414 amino acid propeptide of chicken IL-11R (chIL-11R) was identified. The predicted 414 amino acid sequence showed 42-43% sequence identity with mammalian homologues. In a domain search of the molecule, two fibronectin (FN) type-III domains were identified in the C- terminal portion. On comparison with mammalian IL-11R, 4 conserved cysteine residues and a WSXWS motif were observed within the FN type-III domains. Expression analysis revealed that chIL-11Ralpha is strongly expressed in brain, heart, lung, liver, glandular stomach, kidney, the immature testis, ovary and chicken blastodermal cells (CBCs) after 1-day-cultivation. These findings strongly indicate that the identified chicken cDNA sequence encodes chIL-11R alpha-chain homologue.
authors.affiliation: Hiroshima Prefectural Institute of Industrial Science and Technology, Higashi-Hiroshima, Hirohima 739-0046, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kawashima
authors.forename: Shintaro
authors.initials: S
authors.lastname: Hojyo
authors.forename: Norihisa
authors.initials: N
authors.lastname: Nishimichi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Sato
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Aosasa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Furusawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics*; D053702:Receptors, Interleukin-11
chemical_list: D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11