title: The anti-endotoxic effect of o-aminobenzoic acid from Radix Isatidis.
abstract: OBJECTIVE
  To study the anti-endotoxic effect of o-aminobenzoic acid (OABA) isolated from Banlangen(BLG).
  
  
  METHODS
  OABA was extracted and isolated from BLG and diluted into 0.5% solution. The concentration of endotoxin (ET) pretreated with OABA was quantitatively detected using Limulus test. The inhibition of ET-induced fever by OABA was measured in rabbits. The rates of lipopolysaccharides (LPS)-induced death in mice pretreated with or without OABA were then compared. The influence of OABA on the release of TNF-alpha and NO from macrophages induced by LPS was examined in mice.
  
  
  RESULTS
  After pretreatment with OABA, 84.4% of ET was destroyed. The ET-induced fever in rabbits decreased significantly and the rate of LPS-induced death in mice dropped from 70% to 20%. The release of TNF-alpha and NO induced by LPS in mice was inhibited dose-dependently when the concentration of OABA was between 0.125% and 0.5%.
  
  
  CONCLUSIONS
  OABA isolated from BLG has an anti-endotoxic effect.
authors.affiliation: Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. fjg3560@sina.com
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Fang
authors.forename: Yun-hai
authors.initials: YH
authors.lastname: Liu
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Fang
authors.forename: Hong-gang
authors.initials: HG
authors.lastname: Han
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D005260:Female; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D031219:Isatis / Q000737:chemistry*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D062367:ortho-Aminobenzoates; D009569:Nitric Oxide