title: Preparation of a diclofenac potentiometric sensor and its application to pharmaceutical analysis and to drug recovery from biological fluids.
abstract: A novel diclofenac ion-selective electrode is prepared, characterized and used in pharmaceutical analysis. The diclofenac complex with hexadecylpyridinium bromide is obtained in situ by soaking the PVC-membranes in a 1x10(-2) M diclofenac solution. Among four different solvent mediators tested, dibutyl phthalate (DBP) exhibited a proper behavior including Nernstian slope of the calibration curve, fast response time and good reproducibility of the emf values. The electrode exhibits a Nernstian slope of -59+/-1 mV decade(-1) for diclofenac in the concentration range 1.0x10(-5) to 1.0x10(-2) M with a limit of detection of 4.0x10(-6) M. The electrode displays a good selectivity for diclofenac with respect to a number of common inorganic and organic species. It can be used in a pH range of 6.0-9.0. The membrane sensor was successfully applied to the determination of diclofenac in its tablets as well as for its recovery from blood serum and urine samples.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Razi University, Kermanshah, Iran. mshamsipur@yahoo.com
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Jalali
authors.forename: Sohrab
authors.initials: S
authors.lastname: Ershad
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001826:Body Fluids / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004008:Diclofenac / Q000032:analysis* / Q000138:chemical synthesis; D011199:Potentiometry / Q000379:methods
chemical_list: D004008:Diclofenac