Determination of ceftriaxone in cerebrospinal fluid by ion-pair liquid chromatography.
An ion-pair liquid chromatographic assay was developed and validated for the determination of ceftriaxone in cerebrospinal fluid. Chromatographic separation was achieved on a C18 column (125 x 4 mm, 5 microm) with detection at 270 nm, a 1 mL/min flow rate and a 50 microL loop. The mobile phase consisted of 300 mL acetonitrile, 50 mL 0.1M phosphate buffer (pH 7.4), 3.2 g tetrabutylammonium bromide as the ion-pairing agent, and dilution with distilled deionized water to 1 L. Cephradine was used as the internal standard. The assay was linear for ceftriaxone concentrations of 0.5-50 microg/mL. The coefficients of variation for precision were <4.61%. The accuracy ranged from 96.07 to 102.42%. The detection and quantitation limits were 0.019 and 0.065 microg/mL, respectively. This method was used to quantify ceftriaxone in the cerebrospinal fluid of children with meningitis. The results showed that the method described here is useful for the determination of ceftriaxone in cerebrospinal fluid.
de Diego Glaría
Marta
M
University of Concepción, Pharmacy Department, Pharmacy School, P.O. Box 237, Concepción, Chile. mdediego@udec.cl
Moscciati
Gloria Godoy
GG
Ramos
Ricardo Godoy
RG
D002443:Ceftriaxone / Q000134:cerebrospinal fluid* / Q000627:therapeutic use; D002494:Central Nervous System Infections / Q000134:cerebrospinal fluid; D002511:Cephalosporins / Q000134:cerebrospinal fluid* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000188:drug therapy; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
D002511:Cephalosporins; D007202:Indicators and Reagents; D012996:Solutions; D002443:Ceftriaxone