title: Suppression of invasion of a hamster pancreatic cancer cell line by antisense oligonucleotides mutation-matched to K-ras gene.
abstract: The anti-invasive activity of antisense oligonucleotides (ASO) specific to the K-ras gene in hamster pancreatic cancer was investigated. HaP-T1, a cell culture derived from BHP-induced hamster pancreatic cancer, was used. After liposome-mediated transfection with mutation-matched and mutation-mismatched ASO in different concentrations, cell proliferation was studied by MTT and MTT-agarose methods. In vitro chemoinvasion assay with the reconstitution of a matrix of a basement membrane onto a filter in a Boyden chamber was performed. Mutation-matched ASO inhibited the tumor growth and invasiveness of HaP-T1 in a dose-dependent manner, while mutation-mismatched ASO were not effective in inhibiting invasion. The present study suggests that antisense oligonucleotides mutation-matched to the K-ras gene may be a new anticancer strategy for pancreatic cancer since they inhibited not only tumor growth but also invasiveness in vitro.
authors.affiliation: Department of Surgery, University of São Paulo, São Paulo, Brazil. morioka@gvmail.br
authors.forename: Cintia Yoko
authors.initials: CY
authors.lastname: Morioka
authors.forename: Marcel Cerqueira Cesar
authors.initials: MC
authors.lastname: Machado
authors.forename: Seiji
authors.initials: S
authors.lastname: Saito
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakada
authors.forename: André Siqueira
authors.initials: AS
authors.lastname: Matheus
authors.forename: José
authors.initials: J
authors.lastname: Jukemura
authors.forename: Telesforo
authors.initials: T
authors.lastname: Bacchella
authors.forename: Terumi
authors.initials: T
authors.lastname: Takahara
authors.forename: Akiharu
authors.initials: A
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D006224:Cricetinae; D011905:Genes, ras; D009154:Mutation; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D016376:Oligonucleotides, Antisense