title: Midgut antibodies reduce the reproductive capacity of Anopheles stephensi (Diptera: Culicidae).
abstract: Rabbits immunized with polypeptides of midgut of glucose fed A. stephensi resulted in high titer of antibodies (10(4)-10(6)) as detected by ELISA. Effect of antisera on fecundity, hatchability and engorgement was investigated. Fecundity was reduced drastically (62.4%). Eight polypeptides were recognized by the antisera raised against midgut tissues viz., 92, 85, 55, 52, 45, 38, 29 and 13 kDa. Cross reactivity of these antibodies with different tissues of A. stephensi as well as different species of Anopheles was also analyzed. The results indicated that anti-mosquito midgut antibodies had the potential to disrupt the reproductive physiology of mosquitoes in view of the present study, there is a need for further investigation with target antigens.
authors.affiliation: Department of Biosciences, Maharshi Dayanand University, Rohtak, India. gakharsk@yahoo.co.in
authors.forename: S K
authors.initials: SK
authors.lastname: Gakhar
authors.forename: Amita
authors.initials: A
authors.lastname: Suneja
authors.forename: T
authors.initials: T
authors.lastname: Adak
mesh_terms: D000818:Animals; D000852:Anopheles / Q000254:growth & development* / Q000276:immunology; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology; D004064:Digestive System / Q000276:immunology*; D005260:Female; D007300:Insect Control; D007303:Insect Vectors / Q000254:growth & development* / Q000276:immunology; D011817:Rabbits; D012098:Reproduction / Q000187:drug effects* / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens