Purkinje-cell degeneration in prion protein-deficient mice is associated with a cerebellum-specific Doppel protein species signature.
PrP(c) (cellular prion protein) and Doppel are antagonizing proteins, respectively neuroprotective and neurotoxic. Evidence for Doppel neurotoxicity came from PrP(c)-deficient (Prnp(0/0)) mouse lines developing late onset Purkinje-cell degeneration caused by Doppel overexpression in brain. To address the molecular underpinnings of this cell-type specificity, we generated Doppel N-terminal-specific antibodies and started to examine the spatio-temporal expression of Doppel protein species in Ngsk Prnp(0/0) brain. Although Doppel overexpression is ubiquitous, Western analyses of normal and deglycosylated protein extracts revealed cerebellar patterns distinct from the rest of the brain, supporting the idea that neurotoxicity might be linked to a particular Doppel species pattern. Furthermore, our newly raised antibodies allowed the first Doppel immunohistochemical analyses in brain, showing a distribution in Prnp(0/0) cerebellum similar to PrP(c) in wild type.
Al Bersaoui
Roméo
R
Unité 575 INSERM, Physiopathologie du Système Nerveux, Strasbourg, France.
Robert
Isabelle
I
Lutz
Yves
Y
Blanc
Frédéric
F
Sommermeyer-Leroux
Ghislaine
G
Shibaguchi
Hirotomo
H
Aunis
Dominique
D
Fuchs
Jean-Paul
JP
D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000737:chemistry*; D058851:GPI-Linked Proteins; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009410:Nerve Degeneration / Q000473:pathology*; D016133:Polymerase Chain Reaction; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D011689:Purkinje Cells / Q000473:pathology*; D011994:Recombinant Proteins / Q000378:metabolism
D058851:GPI-Linked Proteins; D011328:Prions; C122036:Prnd protein, mouse; D011994:Recombinant Proteins