The purification and concentration of hog cholera virus.
Partial purification of hog cholera virus (HCV) using a simple batch-type chromatographic procedure with magnetic ferric oxide (MFO) is described. Infectious HCV was adsorbed from isotonic solutions to MFO and was eluted under conditions of low ionic strength and high pH. Aqueous solutions of 0.01 M sodium cyanide or 0.0003 M ammonium hydroxide effectively dissociated MFO-HCV complexes. The data indicate that 50 to 100% of the original HCV infectivity was recovered concomitant with a 90 to 95% reduction of extraneous organic nitrogen.MFO-purified HCV was concentrated by density gradient type centrifugations in buffered solutions of cesium chloride and sucrose. Prolonged isodensity centrifugations of concentrated MFO-purified HCV indicated a buoyant density of 1.14 to 1.15 gm/ml for the strain of virus used.
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D000818:Animals; D002460:Cell Line; D002845:Chromatography / Q000379:methods / Q000662:veterinary; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D005290:Ferric Compounds; D008280:Magnetics; D013552:Swine; D013997:Time Factors
D005290:Ferric Compounds; C000499:ferric oxide