Role of HdeA in acid resistance and virulence in Brucella abortus 2308.
Two-dimensional gel electrophoretic analysis of cell lysates suggests that stationary phase production of wild-type levels of an ortholog of the low pH dependent chaperone HdeA in Brucella abortus 2308 during growth in a minimal medium requires the presence of the RNA binding protein Hfq. Although mutational analysis demonstrated that HdeA contributes to acid resistance in this bacterium, this protein is not required for wild-type virulence in the BALB/c mouse model. These experimental findings indicate that the brucellae rely upon additional gene products to resist the acidic conditions they encounter in the phagosomal compartment of host macrophages.
Valderas
Michelle Wright
MW
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27834, USA.
Alcantara
Rosemarie B
RB
Baumgartner
John E
JE
Bellaire
Bryan H
BH
Robertson
Gregory T
GT
Ng
Wai-Leung
WL
Richardson
John M
JM
Winkler
Malcolm E
ME
Roop
R Martin
RM
D000595:Amino Acid Sequence; D000818:Animals; D002003:Brucella abortus / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D002006:Brucellosis / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D035001:Host Factor 1 Protein / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014774:Virulence
D035001:Host Factor 1 Protein