Hemagglutinating and hemolytic activities of Enterococcus faecalis strains isolated from different human clinical sources.
A total of 95 Enterococcus faecalis strains isolated from different human clinical sources were investigated for hemagglutinating activities and hemolysin (Hly) production in the presence of erythrocytes from a wide range of species. MRHA (mannose-resistant hemagglutination) activity was found in all clinical strains tested in this study. MRHA of E. faecalis strains isolated from different sources was most frequently observed with human (both group O and A) and guinea pig erythrocytes. None of the strains agglutinated horse erythrocytes in the presence of 1% alpha-D-mannose. It should be emphasized that our data indicate the absence of a relationship between sources and MRHA. In contrast, all 95 strains investigated in this report were negative for MSHA (mannose-sensitive hemagglutination) activity. Regarding hemolysin production, it was seen that E. faecalis, and particularly urinary strains, preferably lysed horse erythrocytes. On the other hand, none of the 95 clinical strains tested in this study showed hemolytic activity against bovine and sheep erythrocytes. In general, these results show that E. faecalis strains isolated from different clinical sources possessed a diversity of hemagglutinins and a limited repertoire of hemolysin activities.
Izumi
Erika
E
Laboratório de Patogenicidade Microbiana, Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rod. Celso Garcia Cid (PR 445) s/n Campus Universitário, Londrina, Paraná, CEP 86051-990, Brazil.
Domingues Pires
Patrícia
P
Bittencourt de Marques
Elisa
E
Suzart
Sérgio
S
D000818:Animals; D002417:Cattle; D013293:Enterococcus faecalis / Q000302:isolation & purification / Q000472:pathogenicity*; D004912:Erythrocytes / Q000276:immunology; D010861:Fimbriae, Bacterial; D006386:Hemagglutination Tests; D006460:Hemolysin Proteins / Q000032:analysis; D006461:Hemolysis; D006736:Horses; D006801:Humans; D008358:Mannose; D012756:Sheep; D013045:Species Specificity
D006460:Hemolysin Proteins; D008358:Mannose