Construction of bacterial artificial chromosome libraries for use in phylogenetic studies.
Bacterial artificial chromosome (BAC) libraries are emerging as valuable tools for investigating phylogenetic relationships at the level of genome structure. To date, BAC library construction has been restricted to a fairly small number of laboratories and species that represent a not insignificant, but a fairly small, fraction of diversity in the plant kingdom. This chapter is intended to contribute to rectifying this situation by providing protocols that facilitate BAC library construction in laboratories possessing basic molecular biology skills.
McCubbin
Andrew G
AG
School of Biological Sciences, Washington State University, Pullman, Washington 99164-4236, USA.
Roalson
Eric H
EH
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D005822:Genetic Vectors; D008970:Molecular Weight; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D012685:Sepharose; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D012685:Sepharose