Molecular dynamics simulations of evolved collective motions of atoms in the myosin motor domain upon perturbation of the ATPase pocket.
A crucial point for mechanical force generation in actomyosin systems is how the energy released by ATP hydrolysis in the myosin motor domain gives rise to the movement of the myosin head along the actin filament. We assumed the signal of the ATP hydrolysis to be transmitted as modulated atomic vibrations from the nucleotide-binding site throughout the myosin head, and carried out 1-ns all-atom molecular dynamics simulations for that signal transmission. We distributed the released energy to atoms located around the ATPase pocket as kinetic energies and examined how the effect of disturbance extended throughout the motor domain. The result showed that the disturbance signal extended over the motor domain in 150 ps and induced slowly varying collective motions of atoms at the actin-binding site and the junction with the neck, both of which are relevant to the movement of the myosin head along the actin filament. We also performed a principal component analysis of thermal atomic motions for the motor domain, and the first principal component was consistent with the response to the disturbance given to the ATPase pocket.
Kawakubo
Tatsuyuki
T
Faculty of Engineering, Toin University of Yokohama, Aoba-ku, Japan. tatsu@cc.toin.ac.jp
Okada
Okimasa
O
Minami
Tomoyuki
T
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D001665:Binding Sites / Q000502:physiology; D003198:Computer Simulation; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D009218:Myosins / Q000737:chemistry*; D017434:Protein Structure, Tertiary / Q000502:physiology
D020409:Molecular Motor Proteins; D000251:Adenosine Triphosphatases; D009218:Myosins