title: EBV latent membrane protein 1 abundance correlates with patient age but not with metastatic behavior in north African nasopharyngeal carcinomas.
abstract: BACKGROUND
  Undifferentiated nasopharyngeal carcinomas are rare in a majority of countries but they occur at a high incidence in South China and to a lesser extent in North Africa. They are constantly associated with the Epstein-Barr virus (EBV) regardless of patient geographic origin. In North Africa, the distribution of NPC cases according to patient age is bi-modal with a large group of patients being around 50 years old (80%) and a smaller group below 25 years old. We and others have previously shown that the juvenile form of NPC has distinct biological characteristics including a low amount of p53 and Bcl2 in the tumor tissue and a low level of anti-EBV IgG and IgA in the peripheral blood.
  
  
  RESULTS
  To get more insight on potential oncogenic mechanisms specific of these two forms, LMP1 abundance was assessed in 82 NPC patients of both groups, using immuno-histochemistry and semi-quantitative evaluation of tissue staining. Serum levels of anti-EBV antibodies were simultaneously assessed. For LMP1 staining, we used the S12 antibody which has proven to be more sensitive than the common anti-LMP1 CS1-4 for analysis of tissue sections. In all NPC biopsies, at least a small fraction of cells was positively stained by S12. LMP1 abundance was strongly correlated to patient age, with higher amounts of the viral protein detected in specimens of the juvenile form. In contrast, LMP1 abundance was not correlated to the presence of lymph node or visceral metastases, nor to the risk of metastatic recurrence. It was also independent of the level of circulating anti-EBV antibodies.
  
  
  CONCLUSIONS
  The high amount of LMP1 recorded in tumors from young patients confirms that the juvenile form of NPC has specific features regarding not only cellular but also viral gene expression.
authors.affiliation: Laboratoire d'Anatomie et de Cytologie Pathologiques, Hôpital Universitaire Habib Bourguiba, 3029 Sfax, Tunisia. akabdelmajid@yahoo.fr
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.forename: Hela
authors.initials: H
authors.lastname: Karray
authors.forename: Sandrine
authors.initials: S
authors.lastname: Rodriguez
authors.forename: Mathieu
authors.initials: M
authors.lastname: Rosé
authors.forename: Jamel
authors.initials: J
authors.lastname: Daoud
authors.forename: Mounir
authors.initials: M
authors.lastname: Frikha
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.forename: Jaap
authors.initials: J
authors.lastname: Middeldorp
authors.forename: Rachid
authors.initials: R
authors.lastname: Jlidi
authors.forename: Pierre
authors.initials: P
authors.lastname: Busson
mesh_terms: D000293:Adolescent; D000328:Adult; D000352:Africa, Northern / Q000453:epidemiology; D000368:Aged; D000375:Aging / Q000378:metabolism*; D002648:Child; D005260:Female; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology / Q000473:pathology* / Q000821:virology*; D009362:Neoplasm Metastasis; D014763:Viral Matrix Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins