title: Transient accumulation of gamma-butyrolactone during degradation of bis(4-chloro-n-butyl) ether by diethylether-grown Rhodococcus sp. strain DTB.
abstract: Rhodococcus sp. strain DTB (DSM 44534) grows aerobically on diethylether as sole source of carbon and energy. Dense cell suspension experiments showed that the induced ether-cleaving enzyme system attacks a broad range of ethers like tetrahydrofuran, phenetole and chlorinated alkylethers including Calpha-substituted alkylethers. Identification of metabolites revealed that degradation of the ethers started by an initial attack of the ether bond. Diethylether-grown cells degraded bis(4-chloro-n-butyl) ether via an initial ether scission followed by the transient accumulation of gamma-butyrolactone as intermediate at nearly stoichiometric concentrations.
authors.affiliation: Fachgebiet Technische Biochemie, Technische Universität Berlin, Seestrasse 13, 13353 Berlin, Germany. Marcus.Moreno@tu-berlin.de
authors.forename: M
authors.initials: M
authors.lastname: Moreno-Horn
authors.forename: L-A
authors.initials: LA
authors.lastname: Garbe
authors.forename: R
authors.initials: R
authors.lastname: Tressl
authors.forename: H
authors.initials: H
authors.lastname: Görisch
mesh_terms: D015107:4-Butyrolactone / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D004986:Ether / Q000737:chemistry / Q000378:metabolism*; D004987:Ethers / Q000378:metabolism*; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004987:Ethers; C476675:bis(1-chloro-2-propyl) ether; D004986:Ether; D015107:4-Butyrolactone