Comprehensive two-dimensional gas chromatography coupled to a rapid-scanning quadrupole mass spectrometer: principles and applications.
The principles, practicability and potential of comprehensive two-dimensional (2D) gas chromatography coupled to a rapid-scanning quadrupole mass spectrometer (GC x GC-qMS) for the analysis of complex flavour mixtures in food, allergens in fragrances and polychlorinated biphenyls (PCBs) were studied. With a scan speed of 10,000 amu/s, monitoring over a mass range of up to 200 atomic mass unit (amu) can be achieved at an acquisition frequency of 33 Hz. Extending this mass range and/or increasing the data acquisition frequency results in a loss of spectral quality. Optimal parameter settings allow, next to unambiguous identification/confirmation of target compounds on the basis of high-quality mass spectra, fully satisfactory quantification (three to four modulations per peak) with linear calibration plots and detection limits in the low-pg level. The potential of time-scheduled data acquisition to increase the effective mass range within one GC x GC run was also explored. The analyses, with baseline separation of the flavours, allergens and PCB target compounds, took less than 30 min.
Adahchour
Mohamed
M
Department of Analytical Chemistry and Applied Spectroscopy, Vrije Universiteit, de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands. m.adahchour@few.vu.nl
Brandt
Menno
M
Baier
Hans-Ulrich
HU
Vreuls
René J J
RJ
Batenburg
A Max
AM
Brinkman
Udo A Th
UA
D000485:Allergens / Q000032:analysis; D005421:Flavoring Agents / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D011078:Polychlorinated Biphenyls / Q000032:analysis; D012680:Sensitivity and Specificity
D000485:Allergens; D005421:Flavoring Agents; D000069463:Olive Oil; D010938:Plant Oils; D011078:Polychlorinated Biphenyls