[Preparation of transfusable human universal red blood cell with recombinant alpha-galactosidase].
In order to meet the demand for safe transfusion in special conditions and to utilize the donated blood supply efficiently, technology has been developed to convert erythrocytes from type A, B, or AB to "universal donor" blood. Conversion of blood type B to O was performed by means of recombinant alpha-galactosidase digestion. The results showed that blood type B to O was converted successfully, 1 transfusion unit of red cells of group B (100 ml totally) could converted to universal blood cells in the optimal conditions including pH 5.6, 26 degrees C, 2 hours, obturation and sterilization. It is concluded that the universal red blood cells converted from group B to group O are conformed to demand of identification rules of biological products, no harmful effects of alpha-galactosidase on cell structure and function are observed. The converted red cells can stored in 4 degrees C for 21 days.
Gong
Feng
F
Department of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
Lü
Qiu-Shuang
QS
You
Ying
Y
Gao
Hong-Wei
HW
Bao
Guo-Qiang
GQ
Gao
Xin
X
Li
Su-Bo
SB
Li
Li-Li
LL
Wang
Ying-Li
YL
Tian
Shu-Guang
SG
Zhang
Zhi-Xin
ZX
Zhang
Ping
P
Zhang
Yang-Pei
YP
D000017:ABO Blood-Group System / Q000145:classification / Q000276:immunology*; D001787:Blood Group Incompatibility / Q000517:prevention & control; D001803:Blood Transfusion / Q000379:methods; D003069:Coffee / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007519:Isoantigens / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D000519:alpha-Galactosidase / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D000017:ABO Blood-Group System; D003069:Coffee; D007519:Isoantigens; D011994:Recombinant Proteins; D000519:alpha-Galactosidase