title: Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes.
abstract: DNA sequence of European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae), mitochondrial cytochrome c oxidase I (cox1) and II (cox2) genes were characterized and used for population genetic analysis. Twenty-six point mutations were identified from a 2,156 bp DNA sequence alignment. The frequency of polymorphic cox1DdeI and HaeIII, and cox2 Sau3AI and MspI restriction sites were determined from 1,414 individuals by polymerase chain reaction restriction fragment length polymorphism. Ten haplotypes were observed. A single haplotype was present among 90% of individuals examined, and a HaeIII haplotype was not present in samples from the Atlantic coast. Significant genetic differentiation existed between Atlantic coast and midwestern United States samples, and between sympatric uni- and bivoltine ecotypes. These genetic markers identify regional and ecotype differences in the North American O. nubilalis population.
authors.affiliation: USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, Iowa 50011, USA. coates@iastate.edu
authors.forename: Brad S
authors.initials: BS
authors.lastname: Coates
authors.forename: Douglas V
authors.initials: DV
authors.lastname: Sumerford
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hellmich
mesh_terms: D000818:Animals; D003710:Demography; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D006239:Haplotypes; D019476:Insect Proteins / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology*; D009036:Moths / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV