Characterization of an immortalized oviduct cell line from the cynomolgus monkey (Macaca fascicularis).
To establish reproductive biological techniques in mammals, it is important to understand the growth environment of the embryo. Oviduct epithelial cells are in close proximity to the embryo during pre-implantation development. We, therefore, established an immortalized oviduct epithelial cell line from the cynomolgus monkey, evaluated the usefulness of these cells as feeder cells for embryo culture, and investigated the gene expression of several growth factors and cytokines in the cells. The immortalized cells were positive for the anti-cytokeratin antibody, as determined by immunocytochemistry, indicating that they are epithelial. They also expressed oviductin, which is specific to oviduct epithelial cells, glyceraldehyde-3-phosphate dehydrogenase (control), leukemia inhibitory factor, vascular endothelial growth factor, epidermal growth factor, insulin-like growth factor 1, transforming growth factor beta-2, and interleukin 4. Mouse embryo development was improved when the immortalized cells were used as feeder cells. This cell line is also useful for studying the factors secreted by oviduct epithelial cells.
Okada
H
H
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Tsukuba, Ibaraki, Japan.
Hirose
Y
Y
Manonmani
P
P
Uda
A
A
Ito
M
M
Sankai
T
T
D000818:Animals; D002461:Cell Line, Transformed; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D004847:Epithelial Cells; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008252:Macaca fascicularis; D051379:Mice
D016207:Cytokines; D006133:Growth Substances; D007633:Keratins