Surface modification of microchip input reservoirs for pressure-induced sample injection into microreactors.
Optimization of geometry and surface modification of microchip input reservoirs were performed to achieve uninterferenced pressure-induced sample injection of multiple samples into microreactors using a single syringe pump. Nine samples of 3.5 microL were pipetted onto input reservoirs and loading of PCR mixture into 260 nL microreactors was achieved followed by successful PCR amplification, confirming that no cross-contamination occurs during injection.
Kazakov
Vasily A
VA
Department of Chemistry, St. Petersburg State University, Petergoff, Universitetskij pr. 26, St. Petersburg, 198504, Russia.
Lavrova
Marina V
MV
Ganeev
Alexander A
AA
Moskvin
Leonid N
LN
Slyadnev
Maxim N
MN
D004247:DNA / Q000737:chemistry / Q000235:genetics; D017022:Flow Injection Analysis / Q000295:instrumentation*; D008838:Microcomputers; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation*
D004247:DNA