title: [Usefulness of D-proline in the differentiation of varieties of Cryptococcus neoformans].
abstract: A comparative study was conducted between the assimilation of D-proline and the growth on canavanine-glycine-bromothymol blue (CGB) medium used for the classification of the varieties of Cryptococcus neoformans. In the 86 studied strains, 100% of coincidence between both methods allowed to affirm that 95.34% corresponded to the neoformans var. and the rest (4.65%) to the gattii var. The results obtained corroborated that all the autoctonous clinical isolations up to the present correspond to the. neoformans var. and made possible to suggest the use of D-proline for the initial evaluation of strains, as an alternative and simple method that presented under these conditions high coincidence with the reference method (growth in CGB).
authors.affiliation: Instituto de Medicina Tropical Pedro Kouri, Apartado 601, Marianao 13, Ciudad de La Habana, Cuba. gerardo@ipk.sld.cu
authors.forename: Gerardo
authors.initials: G
authors.lastname: Martínez Machín
authors.forename: Lisset
authors.initials: L
authors.lastname: Barrial de la Rosa
authors.forename: María T
authors.initials: MT
authors.lastname: Illnait Zaragozi
authors.forename: Iliana del C
authors.initials: Idel C
authors.lastname: Valdés Hernández
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Fernindez Andreu
authors.forename: Mayda R
authors.initials: MR
authors.lastname: Perurena Lancha
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Polo Leal
authors.forename: Dianeya
authors.initials: D
authors.lastname: Mendoza Llanes
mesh_terms: D003455:Cryptococcus neoformans / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D011392:Proline / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011392:Proline