Extracellular hsp70 levels in children with septic shock.
OBJECTIVE
To determine whether hsp70 is elevated in the plasma of children with septic shock.


METHODS
Cohort study.


METHODS
Two academic, tertiary pediatric intensive care units.


METHODS
Ninety-four children admitted to the pediatric intensive care unit with septic shock and 24 control children undergoing elective surgical procedures.


METHODS
Venous or arterial blood sampling.


RESULTS
Children admitted to the pediatric intensive care unit with a diagnosis of septic shock were enrolled in the study following written informed consent. The control group consisted of healthy children undergoing elective surgical procedures. Blood samples from children in the septic shock or control groups were obtained within 24 hrs of admission to the pediatric intensive care unit or during the preoperative visit. Samples were collected, centrifuged, and stored at -70 degrees C. The hsp70 levels were measured using a commercially available enzyme-linked immunosorbent assay. Results were analyzed by Wilcoxon's rank sum test. Extracellular hsp70 levels in children with septic shock were significantly elevated compared with control patients (51.6 ng/mL vs. 8.1 ng/mL, respectively, p = .0004).


CONCLUSIONS
Extracellular hsp70 levels are significantly elevated in children with septic shock compared with controls. Given the newly described cell signaling properties of hsp70, these data suggest that extracellular hsp70 may play a role in the host response during septic shock.
Wheeler
Derek S
DS
Department of Pediatrics, University of Cincinnati College of Medicine and Children's Hospital Research Foundation, Cincinnati, OH, USA.
Fisher
Lyle E
LE
Catravas
John D
JD
Jacobs
Brian R
BR
Carcillo
Joseph A
JA
Wong
Hector R
HR
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012772:Shock, Septic / Q000097:blood*
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins