Regulation of the mitogen-activated protein kinases by Brucella spp. expressing a smooth and rough phenotype: relationship to pathogen invasiveness.
By comparing smooth wild-type Brucella spp. to their rough mutants, we show that the LPS O chain restricted the activation of the ERK1/2 and p38 mitogen-activated protein kinase (MAPK) pathways, thus preventing the synthesis of immune mediators that regulate host defense. We conclude that the MAPKs are a target for immune intervention by virulent smooth Brucella.
Jiménez de Bagüés
María P
MP
Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, Ap. 727, 50080 Zaragoza, Spain. mpjimenezdebagues@aragon.es
Gross
Antoine
A
Terraza
Annie
A
Dornand
Jacques
J
D000818:Animals; D002002:Brucella / Q000145:classification / Q000254:growth & development* / Q000472:pathogenicity*; D002006:Brucellosis / Q000382:microbiology; D002460:Cell Line; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010641:Phenotype; D014774:Virulence; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D008070:Lipopolysaccharides; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases