title: [Amplification of EMS1 gene in oral carcinogenesis].
abstract: OBJECTIVE
  To investigate the amplification of EMS1 gene in the carcinogenesis of oral mucosa.
  
  
  METHODS
  A total of 78 subjects, including 30 patients with oral leukoplakia (OLK), 33 with oral squamous cell carcinoma (OSCC), and 15 healthy controls, were studied. By using microdissection method, we obtained normal mucosa, hyperplastic epithelia, mild-dysplastic epithelia, moderate-dysplastic epithelia, severe-dysplastic epithelia and primary OSCC tissue. Then we analyzed EMS1 amplification by using differential PCR.
  
  
  RESULTS
  EMS1 amplification began from moderate-dysplastic epithelia and occurred in 20.0% OLK cases and 57.6% OSCC cases. In the progress of OSCC, no gene amplification was observed in normal tissues, non-dysplastic OLK and mild-dysplastic OLK, while in the cases with metastasis, amplification frequency increased significantly (P = 0.015).
  
  
  CONCLUSIONS
  EMS1 amplification parallels with the progress of oral carcinogenesis, indicating their potential roles in oral carcinogenesis.
authors.affiliation: Department of Oral Medicine, Guanghua School of Stomatology, Sun-Yat Sen University, Guangzhou 510055, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xia
authors.forename: Bing-qi
authors.initials: BQ
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.forename: Qian-ming
authors.initials: QM
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Cai
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D051356:Cortactin / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics* / Q000473:pathology
chemical_list: C495868:CTTN protein, human; D051356:Cortactin