Dynamical changing patterns of glycogen and enzyme histochemical activities in rat liver graft undergoing warm ischemia injury.
OBJECTIVE
To investigate the changing patterns of glycogen and enzyme histochemical activities in rat liver graft under a different warm ischemia time (WIT) and to predict the tolerant time limitation of the liver graft to warm ischemia injury.


METHODS
The rats were randomized into five groups, WIT was 0, 15, 30, 45, 60 min, respectively, and histochemical staining of liver graft specimens was observed. The recovery changes of glycogen and enzyme histochemistry activities were measured respectively 6 and 24 h following liver graft implantation.


RESULTS
The activities of succinic dehydrogenase, cytochrome oxidase, apyrase (Mg++-ATPase) and content of glycogen were decreased gradually after different WIT in a time-dependent manner. The changes were significant when WIT was over 30 min.


CONCLUSIONS
Hepatic injury is reversible within 30 min of warm ischemia injury. Glycogen and enzyme histochemistry activities of liver grafts and their recovery potency after reperfusion may serve as criteria to evaluate the quality of liver grafts.
He
Xiao-Shun
XS
Organ Transplantation Center, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China. xshe@gdvnet.com
Ma
Yi
Y
Wu
Lin-Wei
LW
Wu
Jin-Lang
JL
Hu
Rui-De
RD
Chen
Gui-Hua
GH
Huang
Jie-Fu
JF
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology*; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D006003:Glycogen; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases