[Influence of cytochrome C on apoptosis induced by daunorubicine in acute myeloid leukemia (AML) cells].
The purpose was to study the responses of AML cell treated with cytochrome C and to explore the influence of cytochrome C on apoptosis of AML cell induced by daunorudicine (DNR). The differentiation of AML cell was detected by Wright-Giemsa staining and NBT test, the apoptosis of AML cell was assayed by flow cytometry and fluorescence microscopy. The results showed as follows: (1) different concentrations of cytochrome C could induce different effects on AML cells. Concentration of cytochrome C for differentiation was 10 microl/ml, for apoptosis was 20 microl/ml, and for necrosis was 40 microl/ml. (2) the apoptosis of AML cells decreased with the administration of cytochrome C in 10.0 microg/ml before treating AML cells with DNR (P < 0.01), but no change was shown with the administration of cytochrome C in 20.0 microg/ml (P > 0.05). (3) in reverse sequence, administrating of cytochrome C in 10 microl/ml and 20 microl/ml after treating AML cells with DNR, two different concentrations of cytochrome C could increase the apoptosis of AML cells (P < 0.01). It is suggested that cytochrome C may probably affect the apoptosis of AML cells induced by DNR.
Chen
Ri-Ling
RL
Department of Pediatrics, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China. CRL319@yahoo.com.cn
Chen
Ming-Zhen
MZ
Cai
Kang-Rong
KR
Fang
Xi-Min
XM
Ye
Zhong-Lü
ZL
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045304:Cytochromes c / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005434:Flow Cytometry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D008856:Microscopy, Fluorescence; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D045304:Cytochromes c; D003630:Daunorubicin