Synthesis of all-trans arachidonic acid and its effect on rabbit platelet aggregation.
A simple and high-yielding method to convert natural all-cis PUFA derivatives to the corresponding all-trans geometrical isomers is described. The method is based on the thiyl radical-catalyzed cis-trans isomerization. The all-trans isomer of arachidonic acid was found to cause rabbit platelet aggregation at concentrations higher than 0.1 mM and inhibition of PAF-induced platelet aggregation in a concentration dependent manner with an IC(50) in the micromolar range.
Anagnostopoulos
Dimitris
D
Department of Chemistry, University of Athens, Panepistimiopolis, Greece.
Chatgilialoglu
Chryssostomos
C
Ferreri
Carla
C
Samadi
Abdelouahid
A
Siafaka-Kapadai
Athanassia
A
D000818:Animals; D016718:Arachidonic Acid / Q000138:chemical synthesis* / Q000494:pharmacology*; D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits
D016718:Arachidonic Acid