title: Identification of a peach latent mosaic viroid hairpin able to act as a Dicer-like substrate.
abstract: The ability of several viroids to induce posttranscriptional gene silencing has been demonstrated; however, the structure recognized by the Dicer enzyme(s) responsible for the initiation of this mechanism remains a mystery. Here, we show that the hairpin known to be implicated in the replication of peach latent mosaic viroid has the ability to trigger the Dicer enzyme(s). This domain, which is composed of a succession of several small stems separated by symmetrical bulges, is reminiscent of the precursor micro-RNAs.
authors.affiliation: RNA Group/Groupe ARN, Département de Biochimie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada.
authors.forename: Patricia
authors.initials: P
authors.lastname: Landry
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Perreault
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010936:Plant Extracts; D027861:Prunus / Q000821:virology*; D034622:RNA Interference; D014908:Triticum / Q000201:enzymology; D014772:Viroids / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
chemical_list: D010936:Plant Extracts