Target accessibility dictates the potency of human RISC.
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
Brown
Kirk M
KM
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Chu
Chia-Ying
CY
Rana
Tariq M
TM
D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D042863:Cyclin-Dependent Kinase 9 / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*
D012367:RNA, Viral; D034743:RNA-Induced Silencing Complex; D042863:Cyclin-Dependent Kinase 9