Effect of coadministration of parenteral multivitamins with lipid emulsion on lung remodeling in an animal model of total parenteral nutrition.
Exposure of parenteral multivitamin solutions (MVP) to ambient light generates peroxides and vitamin loss, and induces initiation of fibrosis and a reduced alveolar count in an animal model of total parenteral nutrition (TPN). Adding MVP to the lipid moiety of TPN prevents lipid peroxidation and vitamin loss. The aim of the study was to compare modes of delivery of MVP on lung procollagen mRNA and alveolar counts. Three-day-old guinea pig pups were infused continuously with one of three intravenous solutions: 1) control = dextrose; 2) AA + MVP = MVP given with the dextrose + amino-acid moiety, in a "piggyback" setup with a lipid emulsion mixed close to the infusion site; and 3) LIP + MVP = same as AA + MVP, except that MVP is given with the lipid emulsion. After 4 days, lungs were prepared for alveolar count (intercept technique) and for quantification of the procollagen/beta-actin mRNA ratio (initial step of fibrosis). Data were compared by ANOVA. The procollagen mRNA was lower (P < 0.05) in animals receiving LIP + MVP than those with AA + MVP. But the two modes of admixture of MVP had the same effect on the alveolar counts, which were lower (P < 0.01) than controls. The mode of delivery of TPN affects lung remodeling. Although LIP + MVP protects against the initiation of lung fibrosis, the absence of a beneficial effect on alveolar counts suggests that these features of lung remodeling are not caused by a unique component of TPN. Specific roles of peroxides, components of MVP, and light exposure on lung remodeling need to be explored before LIP + MVP can be recommended as an alternative mode of parenteral vitamin delivery.
Lavoie
Jean-Claude
JC
Research Centre and Neonatal Service, Hôpital Sainte-Justine, University of Montreal, Montreal, Quebec, Canada. jean-claude.lavoie@umontreal.ca
Rouleau
Thérèse
T
Chessex
Philippe
P
D000596:Amino Acids / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D005947:Glucose / Q000008:administration & dosage; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011347:Procollagen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014815:Vitamins / Q000008:administration & dosage*
D000596:Amino Acids; D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous; D011347:Procollagen; D012333:RNA, Messenger; D014815:Vitamins; D005947:Glucose