Structural organization and cellular localization of tuftelin-interacting protein 11 (TFIP11).
Tuftelin-interacting protein (TFIP11) was first identified in a yeast two-hybrid screening as a protein interacting with tuftelin. The ubiquitous expression of TFIP11 suggested that it might have other functions in non-dental tissues. TFIP11 contains a G-patch, a protein domain believed to be involved in RNA binding. Using a green fluorescence protein tag, TFIP11 was found to locate in a novel subnuclear structure that we refer to as the TFIP body. An in vivo splicing assay demonstrated that TFIP11 is a novel splicing factor. TFIP11 diffuses from the TFIP body following RNase A treatment, suggesting that the retention of TFIP11 is RNA dependent. RNA polymerase II inhibitor (-amanitin and actinomycin D) treatment causes enlargement in size and decrease in number of TFIP bodies, suggesting that TFIP bodies perform a storage function rather than an active splicing function. The TFIP body may therefore represent a new subnuclear storage compartment for splicing components.
Wen
X
X
Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA room 103, Los Angeles, California, 90033-1004, USA.
Lei
Y-P
YP
Zhou
Y L
YL
Okamoto
C T
CT
Snead
M L
ML
Paine
M L
ML
D000256:Adenoviridae / Q000235:genetics; D017398:Alternative Splicing; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D042581:Intranuclear Space / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D014162:Transfection; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
D000546:Amanitins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D011993:Recombinant Fusion Proteins; C485860:TFIP11 protein, human; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins; D012319:RNA Polymerase II; D012259:Ribonuclease, Pancreatic