title: Antigenotoxic potential of glucomannan on four model test systems.
abstract: Antimutagenic, anticlastogenic, and bioprotective effect of polysaccharide glucomannan (GM) isolated from Candida utilis was evaluated in four model test systems. The antimutagenic effect of GM against 9-aminoacridine (9-AA)- and sodium azide (NaN3)-induced mutagenicity was revealed in the Salmonella typhimurium strains TA97 and TA100, respectively. GM showed anticlastogenic effect against N-nitroso-N'-methylurea (NMU) induced chromosome aberrations in the Vicia sativa assay. The bioprotective effect of GM co-treated with methyl-methane-sulphonate (MMS) was also established in Chlamydomonas reinhardtii repair deficient strains uvs10 and uvs14. The statistically significant antimutagenic potential of GM was not proved against 4-nitro-quinoline-1-oxide (4-NQO)-induced mutagenicity in Saccharomyces cerevisiae D7 assay. It may be due to bioprotectivity of alpha-mannan and beta-glucan, which are integral part of S. cerevisiae cell walls. Due to the good water solubility, low molecular weight (30 kDa), antimutagenic/anticlastogenic, and bioprotective activity against chemical compounds differing in mode of action, GM appears to be a promising natural protective (antimutagenic) agent.
authors.affiliation: Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic. vlckova@fns.uniba.sk
authors.forename: V
authors.initials: V
authors.lastname: Vlcková
authors.forename: V
authors.initials: V
authors.lastname: Dúhová
authors.forename: S
authors.initials: S
authors.lastname: Svidová
authors.forename: A
authors.initials: A
authors.lastname: Farkassová
authors.forename: S
authors.initials: S
authors.lastname: Kamasová
authors.forename: D
authors.initials: D
authors.lastname: Vlcek
authors.forename: G
authors.initials: G
authors.lastname: Kogan
authors.forename: P
authors.initials: P
authors.lastname: Rauko
authors.forename: E
authors.initials: E
authors.lastname: Miadoková
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000585:Aminacrine / Q000494:pharmacology; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D002175:Candida / Q000737:chemistry; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D002869:Chromosome Aberrations / Q000187:drug effects; D003434:Crossing Over, Genetic / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D008351:Mannans / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008770:Methylnitrosourea / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D019810:Sodium Azide / Q000494:pharmacology; D033022:Vicia sativa / Q000166:cytology / Q000187:drug effects / Q000235:genetics
chemical_list: D016587:Antimutagenic Agents; D008351:Mannans; D009153:Mutagens; C022901:(1-6)-alpha-glucomannan; D015112:4-Nitroquinoline-1-oxide; D008770:Methylnitrosourea; D000585:Aminacrine; D019810:Sodium Azide; D008741:Methyl Methanesulfonate