c-Abl tyrosine kinase regulates c-fos gene expression via phosphorylating RNA polymerase II.
c-Abl tyrosine kinase, predominantly distributed in nucleus, has been implicated in many important cellular processes including the regulation of gene transcription. In this study, we showed that c-Abl promoted the transcription of c-fos gene, both exogenously and endogenously. The nuclear localization and tyrosine kinase activity of c-Abl were required for the activation of c-fos gene. c-Abl was associated with RNA polymerase II (RNAP II) in vivo and augmented the tyrosine phosphorylation of the largest subunit of RNAP II. In addition, c-Abl and RNAP II could be recruited to the region of c-fos promoter. The combined results suggest that c-Abl plays an important role in the transcriptional regulation of c-fos gene and the tyrosine phosphorylation of the largest subunit of RNAP II by c-Abl is involved in the regulating process.
Jing
Yuqi
Y
Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
Song
Zhaoxia
Z
Wang
Min
M
Tang
Wen
W
Hao
Shui
S
Zeng
Xianlu
X
D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D005786:Gene Expression Regulation; D016762:Genes, fos / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D016315:Proto-Oncogene Proteins c-abl / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
D021122:Protein Subunits; D019000:Phosphotyrosine; D016315:Proto-Oncogene Proteins c-abl; D012319:RNA Polymerase II