title: [The effect of short hairpin RNA on hTERT expression].
abstract: OBJECTIVE
  To study the effect of short hairpin RNA (shRNA) on hTERT expression.
  
  
  METHODS
  Oligonucleotides encoding shRNA against hTERT was cloned into a mammalian shRNA expression vector pUC18U6 to form pUC18U6ht which was transfected into HepG2 cells by using liposome. HepG2 cells transfected by pUC18U6 and pUC18U6GFPsir which expressed shRNA against green fluorescent protein were used as controls. hTERT mRNA in the transfected cells was quantified by using real-time fluorescent RT-PCR.
  
  
  RESULTS
  Compared with the controls, the short hairpin RNA against hTERT decreased the hTERT mRNA level by 49% (P<0.05).
  
  
  CONCLUSIONS
  hTERT expression was reduced by the shRNA.
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital of Xi'an Jiaotong University, Xi'an 710001, China. ldhljgy@fmmu.edu.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Tian-bao
authors.initials: TB
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qi-pei
authors.initials: QP
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D055029:Inverted Repeat Sequences; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012313:RNA; C509186:TERT protein, human; D019098:Telomerase