title: The effects of 3-methylcholanthrene on lymphocyte proliferation in the common carp (Cyprinus carpio L.).
abstract: The sensitivity of lymphocyte proliferation as bioindicator of pollution stress was evaluated in the common carp (Cyrinus carpio L.). The time course response of peripheral blood leukocyte proliferation in response or not to mitogens was measured from 1 to 7 days after peritoneal injection of 3-methylcholantrene (3-MC), and compared to the time course response of a highly sensitive biomarker, induction of cytochrome P450. 3-Methylcholanthrene (40 mg kg(-1)) inhibited both B- and T-lymphocyte proliferation in response to lipopolysaccharide (LPS) and concanavalin A (Con A). Studies with alpha-naphtofiavone, suggest the lack of metabolic processes. 3-Methylcholanthrene alone strongly stimulated resting peripheral blood leukocytes (PBLs) proliferation. This effect was not transient. The induction of lymphocyte proliferation paralleled the increase in cytochrome P450 content in the liver. The specificity of polycyclic aromatic hydrocarbon (PAH)-induced lymphocyte proliferation suggests that this immune activity may be an early marker of exposure to PAHs in aquatic environments. The capacity of 3-MC to induce rapid lymphocyte proliferation may be related to PAH-induced rapid clonal expansion in mammals. These results strongly suggested that the underlying mechanism might be the same in both models. More studies are needed in fish to explain this phenomenon and may be helpful in understanding the occurrence of neoplastic epizootics in fish associated with PAH exposition.
authors.affiliation: Laboratory of General and Comparative Immunophysiology, UER of Sciences, 123, Av. Albert Thomas, 87060 Limoges, France. steph.reynaud@caramail.com
authors.forename: S
authors.initials: S
authors.lastname: Reynaud
authors.forename: P
authors.initials: P
authors.lastname: Deschaux
mesh_terms: D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D002347:Carps / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D008748:Methylcholanthrene / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon / Q000037:antagonists & inhibitors; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D001571:Benzoflavones; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D008934:Mitogens; D018336:Receptors, Aryl Hydrocarbon; D008748:Methylcholanthrene; C011512:alpha-naphthoflavone; D003577:Cytochrome P-450 Enzyme System