Cloning and expression of a novel isoform of heterogeneous nuclear ribonucleoprotein-R.
In the present study, we cloned a novel isoform of heterogeneous nuclear ribonucleoprotein-R (hnRNP-R), hnRNP-R2. Compared with the previously reported hnRNP-R (hnRNP-R1), it lacks the exon 5. Two proteins are both nuclear. The expression of hnRNP-R1 is not tissue-specific and is strikingly higher than that of hnRNR-R2. In comparison, the expression of hnRNP-R2 is low and neural-specific. The expression of two isoforms is differentially regulated during brain development. The results suggest that hnRNP-R may contribute to the regulation of neural development through the variation of hnRNP-R components in the hnRNP complex.
Huang
Jia
J
Laboratory of Genomic Physiology and Center for Brain Research, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, People's Republic of China.
Chen
Xian-hua
XH
Wu
Kun
K
Xu
Ping
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology* / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D012322:RNA Precursors / Q000235:genetics
C439930:HNRNPR protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D020033:Protein Isoforms; D012322:RNA Precursors; D049452:Green Fluorescent Proteins