title: Design of peptides for thin films, coatings and microcapsules for applications in biotechnology.
abstract: A highly-interdisciplinary approach has been developed for minimizing the immunogenicity of films, coatings, microcapsules and other nano-structured materials fabricated from designed polypeptide chains. It is to base the amino-acid sequences on solvent-exposed regions in the folded states of proteins from the same organism. Each such region that meets defined criteria with respect to charge is called a sequence motif. The approach becomes more specifically tailored for intravenous applications by requiring an employed sequence motif to correspond to a known blood protein. An algorithm has been developed to identify sequence motifs in protein-encoding regions of a genome. This work is focused on sequence motifs of charge per unit length >0.5 at neutral pH. It has been found that the number of unique sequence motifs meeting this criterion in available human genome data is maximal for motifs of approx. 7 residues in length. We have designed polypeptides on the basis of computational analysis and shown that they can be used to fabricate nano-structured thin films by electrostatic layer-by-layer assembly (ELBL). The results of this work are discussed with a view to possible applications in biotechnology, notably development of biocompatible coatings and microcapsules.
authors.affiliation: Computational Analysis and Modeling, College of Engineering and Science, Louisiana Tech University, Ruston, LA 71272, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Donald T
authors.initials: DT
authors.lastname: Haynie
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Kaustubh
authors.initials: K
authors.lastname: Sabnis
authors.forename: Vinay
authors.initials: V
authors.lastname: Surpuriya
authors.forename: Nikhil
authors.initials: N
authors.lastname: Pargaonkar
authors.forename: Gyanesh
authors.initials: G
authors.lastname: Sharma
authors.forename: Kranthi
authors.initials: K
authors.lastname: Vistakula
mesh_terms: D020816:Amino Acid Motifs; D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002214:Capsules / Q000737:chemistry*; D015195:Drug Design; D016678:Genome; D015894:Genome, Human; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D015202:Protein Engineering; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry; D012997:Solvents; D055672:Static Electricity
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D010455:Peptides; D011108:Polymers; D011506:Proteins; D012997:Solvents