title: [Study on peparation of anemonin from Ranunculus japonicus Thunb].
abstract: OBJECTIVE
  To study the preparation of anemonin from Ranunculus japonicus Thunb.
  
  
  METHODS
  The best conditions of preparation for anemonin were optimized with monosyllabic experiments, such as using the purity of anemonin as a index for best selection of dying tempreture and the extraction ratio as another index for the concentration of NaCl and extracting times. The structures were clucidated on the basis of UV, IR, 1HNMR, 13CNMR, 1H-1H COSY, HMQC and HMQC spectra and chemical reactions and the purity is measured by HPLC.
  
  
  RESULTS
  The best conditions for the extraction techniques of anemonin were 15% NaCl, four extracting times, 60 degrees C (0.08 Mpa) for drying. The purity of anemonin was 99% and yield rate was 0. 72 per thousand. Its quality was controlled.
  
  
  CONCLUSIONS
  The preparation techniques are feasible and convenient.
authors.affiliation: The Key Lab of Chemistry for Natural Products of Chinese Academy of Sciences in Guizhou Province, Guiyang 550002.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shixi
authors.initials: S
authors.lastname: Hu
authors.forename: Shiqing
authors.initials: S
authors.lastname: Hu
authors.forename: Guangyi
authors.initials: G
authors.lastname: Liang
mesh_terms: D029681:Arabidopsis Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D005663:Furans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031947:Ranunculus / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
chemical_list: D029681:Arabidopsis Proteins; D004365:Drugs, Chinese Herbal; D005663:Furans; C028304:anemonin