Severe cognitive impairment correlates with higher cerebrospinal fluid levels of lactate and pyruvate in a canine model of senile dementia.
Diagnosis of dementia of the Alzheimer's type depends on clinical criteria and exclusion of other disorders because, at this time, a validated biological marker, aside from histological brain examination, remains to be established. The canine counterpart of senile dementia of the Alzheimer type (ccSDAT) is considered a promising model for examining behavioral, cellular and molecular processes involved in early phases of human brain aging and Alzheimer disease (AD). In order to investigate the first events taking place in canine cognitive dysfunction, in this paper we established a new and rapid behavioral test that finely discriminates the degrees of cognitive impairment. Cerebrospinal fluid (CSF) analysis was performed to determine the relationship between each disease stage and modification of cerebral energy metabolism. Our results demonstrate a parallel increase of lactate, pyruvate and potassium concentrations in the severe cognitive deficit. These differences are discussed in view of the neuroprotective role presently given to lactate.
Pugliese
Marco
M
Unitat de Bioquímica, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain.
Carrasco
Josep Lluis
JL
Andrade
Carmen
C
Mas
Ernesto
E
Mascort
Joan
J
Mahy
Nicole
N
D000375:Aging / Q000523:psychology; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000523:psychology*; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D015415:Biomarkers; D001923:Brain Chemistry / Q000502:physiology; D002556:Cerebrospinal Fluid Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003072:Cognition Disorders / Q000134:cerebrospinal fluid* / Q000523:psychology*; D004285:Dogs; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D007656:Ketoglutaric Acids / Q000134:cerebrospinal fluid; D019344:Lactic Acid / Q000134:cerebrospinal fluid*; D008297:Male; D019289:Pyruvic Acid / Q000134:cerebrospinal fluid*; D013056:Spectrophotometry, Ultraviolet
D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D007656:Ketoglutaric Acids; D019344:Lactic Acid; D019289:Pyruvic Acid