title: Use of in vivo-induced antigen technology for identification of Escherichia coli O157:H7 proteins expressed during human infection.
abstract: Using in vivo-induced antigen technology (IVIAT), a modified immunoscreening technique that circumvents the need for animal models, we directly identified immunogenic Escherichia coli O157:H7 (O157) proteins expressed either specifically during human infection but not during growth under standard laboratory conditions or at significantly higher levels in vivo than in vitro. IVIAT identified 223 O157 proteins expressed during human infection, several of which were unique to this study. These in vivo-induced (ivi) proteins, encoded by ivi genes, mapped to the backbone, O islands (OIs), and pO157. Lack of in vitro expression of O157-specific ivi proteins was confirmed by proteomic analysis of a mid-exponential-phase culture of E. coli O157 grown in LB broth. Because ivi proteins are expressed in response to specific cues during infection and might help pathogens adapt to and counter hostile in vivo environments, those identified in this study are potential targets for drug and vaccine development. Also, such proteins may be exploited as markers of O157 infection in stool specimens.
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA. ikudva@partners.org
authors.forename: Manohar
authors.initials: M
authors.lastname: John
authors.forename: Indira T
authors.initials: IT
authors.lastname: Kudva
authors.forename: Robert W
authors.initials: RW
authors.lastname: Griffin
authors.forename: Allen W
authors.initials: AW
authors.lastname: Dodson
authors.forename: Bethany
authors.initials: B
authors.lastname: McManus
authors.forename: Bryan
authors.initials: B
authors.lastname: Krastins
authors.forename: David
authors.initials: D
authors.lastname: Sarracino
authors.forename: Ann
authors.initials: A
authors.lastname: Progulske-Fox
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hillman
authors.forename: Martin
authors.initials: M
authors.lastname: Handfield
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Tarr
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Calderwood
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism*; D003470:Culture Media; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015698:Genomic Library; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D020543:Proteome
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media; D029968:Escherichia coli Proteins; D007106:Immune Sera; D020543:Proteome