title: Expression of matrix metalloproteinases (MMPs) in cultured hepatocellular carcinoma (HCC) cells and surgically resected HCC tissues.
abstract: Matrix metalloproteinases (MMPs) relate to the growth and infiltration of cancer cells, but the frequency and amount of their expression are not yet fully examined in hepatocellular carcinoma. Expression of MMPs (MMP-2, MMP-7, MMP-9, MT1-MMP, MT2-MMP, MT3-MMP) and tissue inhibitors of metalloproteinase (TIMP: TIMP-1, TIMP-2) was investigated on cultured hepatocellular carcinoma (HCC) cells and surgically resected HCC tissues. The cultured cells and tissues expressed MMPs and TIMPs at various degrees, and high expression was observed for MMP-2, MMP-9, MT1-MMP and TIMP-2. Expression of MMP-7, MT2-MMP and TIMP-1 was found at a low frequency and a low amount in both the cells and the tissues. MMP-2 was expressed in various cells: HCC cells, vascular wall and sinusoidal endothelial cells in the cancer area of surgically resected tissues; and hepatocytes, bile duct cells, vascular wall, macrophages and Kupffer cells in the non-cancerous area. MMPs and TIMPs were expressed at a relatively high frequency in hepatocytes of the cancerous area and surrounding non-cancerous area as well as in the other cells and tissues. MMPs and TIMPs may be involved in the progression of hepatocellular carcinoma including the infiltration of cancer cells.
authors.affiliation: Department of Pathology, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan. sachiko@med.kurume-u.ac.jp
authors.forename: Sachiko
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Yano
authors.forename: Seiya
authors.initials: S
authors.lastname: Momosaki
authors.forename: Naoyo
authors.initials: N
authors.lastname: Nishida
authors.forename: Yumi
authors.initials: Y
authors.lastname: Takemoto
authors.forename: Sakiko
authors.initials: S
authors.lastname: Kojiro
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kojiro
mesh_terms: D001652:Bile Ducts / Q000201:enzymology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000601:surgery; D042783:Endothelial Cells / Q000201:enzymology; D022781:Hepatocytes / Q000201:enzymology; D006801:Humans; D007728:Kupffer Cells / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology* / Q000601:surgery; D008264:Macrophages / Q000201:enzymology; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases