title: Comparison of solid-phase microextraction, supercritical fluid extraction, steam distillation, and solvent extraction techniques for analysis of volatile consituents in Fructus Amomi.
abstract: Four sampling techniques, solid-phase microextraction (SPME), supercritical fluid extraction (SFE), steam distillation (SD), and solvent extraction (SE), were compared for the analysis of volatile constituents from a traditional Chinese medicine (TCM) of the dried ripe fruit of Fructus Amomi (Sha Ren). A total of 38 compounds were identified by gas chromatography/mass spectrometry. Different SFE and SPME parameters (modifier content, extraction pressure, and temperature for SFE and fibers, extraction temperature, and time for SPME) were studied. The results by SFE and SPME were compared with those obtained by conventional SD and SE methods. The results showed that SFE and SPME are better sample preparation techniques than SD and SE. Due to SFE's requirement for expensive specialized instrumentation, the simplicity, low cost, and speed of SPME make it a more appropriate technique for extraction of volatile constituents in TCMs.
authors.affiliation: Fudan University, Department of Chemistry, Shanghai 200433, People's Republic of China.
authors.forename: Shun
authors.initials: S
authors.lastname: Shen
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Sha
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Daxi
authors.initials: D
authors.lastname: Fu
authors.forename: Jiakuan
authors.initials: J
authors.lastname: Chen
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D035801:Amomum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009821:Oils / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents; D014835:Volatilization
chemical_list: D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D009821:Oils; D010936:Plant Extracts; D012997:Solvents