title: Thermostabilized ovalbumin that occurs naturally during development accumulates in embryonic tissues.
abstract: We have reported that ovalbumin accumulates without digestion in various tissues during embryonic development of the chicken. There are different types of ovalbumin with respect to thermal stability and one of them, which was named "HS-ovalbumin" in the present study, was found to have a T(m) value of 83 degrees C and to be present dominantly in albumen, egg yolk, amniotic fluid, and serum of fertilized eggs. HS-ovalbumin, arising physiologically from its native form (N-ovalbumin), is reminiscent of the previously described intermediate form appearing during the production processes of the so-called S-ovalbumin, which disappeared shortly in fertilized eggs. We showed that HS-ovalbumin is distinguishable from S-ovalbumin by a monoclonal antibody and also from N-ovalbumin by the stability to heating. At the late stages of development, ovalbumin of amniotic fluid seems to be swallowed through pharynx, carried in the intestine through stomach, and absorbed in the blood. Analyses by monoclonal antibody and heat treatment indicated that the HS-form occupies the largest fraction of ovalbumin that accumulates in the embryonic tissues. The current findings suggest that HS-ovalbumin is crucial for embryogenesis.
authors.affiliation: Department of Bioscience and Biochemistry, the United Graduate School of Agricultural Sciences, Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinohara
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Kanako
authors.initials: K
authors.lastname: Matsuo
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Aoki
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Tatsuzo
authors.initials: T
authors.lastname: Oka
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kurisaki
authors.forename: Koko
authors.initials: K
authors.lastname: Mizumachi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Katsumi
authors.initials: K
authors.lastname: Koga
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002152:Calorimetry, Differential Scanning; D002642:Chick Embryo / Q000378:metabolism*; D006358:Hot Temperature; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
chemical_list: D010047:Ovalbumin