T-cell activation and memory phenotypes in cerebrospinal fluid during HIV infection.
We characterized T cell phenotypes in 74 paired blood and cerebrospinal fluid (CSF) samples of HIV-infected and uninfected persons using four-color flow cytometry. CD4+ and CD8+ T cells subsets were further characterized by identifying activated/resting and memory/naive subsets in CSF and blood using the markers CD38/HLA-DR and CD45RA/CD62L, respectively. With and without HIV-infection, the proportion of CD4+ T cells and memory T cells among T cells in CSF was higher compared to blood. In HIV-infection, activated CD4+ and CD8+ T cells in CSF were more abundant than in uninfected controls. As expected, combination antiretroviral therapy (ART) reduced T cell activation in CSF and blood.
Neuenburg
Jutta K
JK
Department of Neurology, San Francisco General Hospital, General Clinical Research Center at the University of California, San Francisco, CA 94158, USA. juttan@itsa.ucsf.edu
Cho
Tracey A
TA
Nilsson
Annelie
A
Bredt
Barry M
BM
Hebert
Samuel J
SJ
Grant
Robert M
RM
Price
Richard W
RW
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000134:cerebrospinal fluid* / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012367:RNA, Viral / Q000097:blood / Q000134:cerebrospinal fluid; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology*; D019562:Viral Load
D012367:RNA, Viral