title: Frequency and irradiation time-dependant antiproliferative effect of low-power millimeter waves on RPMI 7932 human melanoma cell line.
abstract: The biological effects produced by low power millimeter waves (MMW) were studied on the RPMI 7932 human melanoma cell line. Three different frequency-type irradiation modes were used: the 53.57-78.33 GHz wide-band frequency range, the 51.05 GHz and the 65.00 GHz monochromatic frequencies. In all three irradiation conditions, the radiation energy was low enough not to increase the temperature of the cellular samples. Three hours of radiation treatment, applied every day to the melanoma cell samples, were performed at each frequency exposure condition. The wide-band irradiation treatment effectively inhibited cell growth, while both the monochromatic irradiation treatments did not affect the growth trend of RPMI 7932 cells. A light microscopy analysis revealed that the low-intensity wide-band millimeter radiation induced significant morphological alterations on these cells. Furthermore, a histochemical study revealed the low proliferative state of the irradiated cells. This work provides further evidence of the antiproliferative effects on tumor cells induced by low power MMW in the 50-80 GHz frequency range of the electromagnetic spectrum.
authors.affiliation: Department of Chemistry, University of Calabria, 87036 Arcavacata di Rende (CS), Italy. beneduci@unical.it
authors.forename: Amerigo
authors.initials: A
authors.lastname: Beneduci
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Chidichimo
authors.forename: Renata
authors.initials: R
authors.lastname: De Rose
authors.forename: Luigi
authors.initials: L
authors.lastname: Filippelli
authors.forename: Serafina V
authors.initials: SV
authors.lastname: Straface
authors.forename: S
authors.initials: S
authors.lastname: Venuta
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D004574:Electromagnetic Fields; D006801:Humans; D008027:Light; D008545:Melanoma / Q000473:pathology / Q000532:radiotherapy*; D008853:Microscopy; D008872:Microwaves