[Effects of carnitine acetyltransferase gene knockout on long chain dicarboxylic acid production and metabolism of Candida tropicalis].
Candida tropicalis can assimilate n-alkane as a sole carbon source and produce dicarboxylic acids (DCAs). The synthesis of DCAs is thought to be reduced by beta-oxidation. Carnitine acetyltransferase (CAT) is the major enzyme to transfer DCAs into beta-oxidative pathway, then DCAs would be catalyzed to generate ATP to supply cells with energy. A homologous recombination plasmid was constructed, in which CAT gene was disrupted by inserting hygromycin B resistance gene. This plasmid was used to transform Candida tropicalis wild type strain F10-1, and one single CAT gene knockout strain was obtained. Comparing with the wild type, the recombinant increased DCA13 yield and molar conversion of alkane by 13.0% and 11.8%, respectively, and decreased unnecessary consumption of DCAs in beta-oxidation.
Gao
Hong
H
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. gaohong00@mails.tsinghua.edu.cn
Zhang
Jian
J
Hua
Yu-tao
YT
Li
Chun
C
Cao
Zhu-an
ZA
D041022:Candida tropicalis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D002332:Carnitine O-Acetyltransferase / Q000235:genetics*; D003998:Dicarboxylic Acids / Q000378:metabolism*; D025141:Drug Resistance, Fungal / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D010957:Plasmids; D011995:Recombination, Genetic
D003998:Dicarboxylic Acids; D006921:Hygromycin B; D002332:Carnitine O-Acetyltransferase