Improvements in europium sensitized fluorimetric determination of demeclocycline and methacycline.
Demeclocycline (DM) and methacycline (MT) have been determined by europium-sensitized fluorescence, using EDTA as co-ligand and cetyltrimethylammonium chloride as surfactant. The methods have been developed in slightly alkaline solutions, with the formation of a new chelate where the lanthanide ion is bound to the beta-diketone group. Calibration graphs between 0.01 and 0.1 microg mL(-1) have been obtained for DM and MT determination. Both methods have been applied to the determination of these tetracyclines in serum samples with satisfactory recovery results.
Mahedero
M C
MC
Department of Analytical Chemistry, University of Extremadura, 06071 Badajoz, Spain. mahedero@unex.es
Bohoyo
D
D
Salinas
F
F
Ardila
T
T
Airado
D
D
Roldán
B
B
D003707:Demeclocycline / Q000032:analysis*; D005063:Europium / Q000032:analysis*; D008690:Methacycline / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005063:Europium; D003707:Demeclocycline; D008690:Methacycline