title: Intracellular cAMP controls a physical association of V-1 with CapZ in cultured mammalian endocrine cells.
abstract: V-1, an ankyrin repeat protein with the activity to control tyrosine hydroxylase (TH) gene expression and transmitter release in PC12D cells, associates with CapZ, an actin capping protein, and thereby regulates actin polymerization in vitro. In this study, immunoprecipitation and Western blot analysis showed that V-1 was physically associated with CapZ-beta in PC12D transfectants overexpressing V-1. These proteins were co-localized in the soma of Purkinje cells of rat cerebellum as assayed by immunohistochemistry. Furthermore, in the V-1 transfectants, the amount of CapZ which physically associated with V-1 was steeply reduced at 2h after treatment with forskolin, but was thereafter increased to reach its initial level at 12h after forskolin-treatment. These results suggest that the association of V-1 with CapZ is controlled by a cAMP-dependent signalling pathway probably to play a functional role in the regulatory mechanism of actin dynamics in the endocrine system and the central nervous system.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kitazawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamakuni
authors.forename: Si-Young
authors.initials: SY
authors.lastname: Song
authors.forename: Chieko
authors.initials: C
authors.lastname: Kato
authors.forename: Reiko
authors.initials: R
authors.lastname: Tsuchiya
authors.forename: Mami
authors.initials: M
authors.lastname: Ishida
authors.forename: Nobuhide
authors.initials: N
authors.lastname: Suzuki
authors.forename: Eijiro
authors.initials: E
authors.lastname: Adachi
authors.forename: Shintaro
authors.initials: S
authors.lastname: Iwashita
authors.forename: Susumu
authors.initials: S
authors.lastname: Ueno
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yanagihara
authors.forename: Masato
authors.initials: M
authors.lastname: Taoka
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Isobe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D051347:CapZ Actin Capping Protein; D002460:Cell Line; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D000242:Cyclic AMP / Q000378:metabolism*; D004703:Endocrine System / Q000166:cytology / Q000378:metabolism*; D047468:Immunoprecipitation; D008840:Microfilament Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D011689:Purkinje Cells / Q000737:chemistry; D051381:Rats
chemical_list: D051347:CapZ Actin Capping Protein; D008840:Microfilament Proteins; D009124:Muscle Proteins; D000242:Cyclic AMP