title: Evaluation of the Bio-Rad VARIANT II HbA2/HbA1C Dual Program for measurement of hemoglobin concentrations and detection of variants.
abstract: In this work data obtained on the VARIANT II hemoglobin analyzer using the Dual Kit elution system were compared to those obtained with the beta-Thalassemia Short Program. Since many laboratories still use an earlier model of the hemoglobin analyzer, the Variant 1, these data were also compared to those obtained with the latter instrument. Our study is divided into two parts. The first is an evaluation of the precision of the VARIANT II for determining the levels of hemoglobin (Hb)A2 and Hb F. This was carried out for normal subjects, thalassemic patients and patients with HbS. The second part concerns the potential of this instrument in the presumptive identification of Hb variants.
authors.affiliation: Service de Biochimie Génétique, Hôpital Henri Mondor, Créteil, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Riou
authors.forename: Christian
authors.initials: C
authors.lastname: Godart
authors.forename: Mireille
authors.initials: M
authors.lastname: Mathis
authors.forename: Didier
authors.initials: D
authors.lastname: Hurtrel
authors.forename: Henri
authors.initials: H
authors.lastname: Wajcman
authors.forename: Claude
authors.initials: C
authors.lastname: Préhu
authors.forename: Josiane
authors.initials: J
authors.lastname: Bardakdjian
mesh_terms: D005319:Fetal Hemoglobin / Q000032:analysis*; D014644:Genetic Variation; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006443:Hemoglobin A2 / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis
chemical_list: D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; D006443:Hemoglobin A2; D006454:Hemoglobins; D011933:Reagent Kits, Diagnostic