title: Apoptosis repressor with caspase recruitment domain (ARC) is expressed in cancer cells and localizes to nuclei.
abstract: Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic and non-neurogenic human and rat cancer cell lines. Unexpectedly, ARC was localized almost exclusively to the nuclei of cancer cells, which was unlike the cytoplasmic localization of ARC in non-cancer cells. Furthermore, nuclear ARC in cancer cells did not co-localize with nucleolus protein of 30 kDa, an alternatively spliced ARC isoform. These findings indicate that ARC is distributed differently in cancer cells than non-cancer cells and thus might play a role in neoplastic transformation.
authors.affiliation: Department of Anatomy and Case Comprehensive Cancer Center, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Mi
authors.initials: M
authors.lastname: Wang
authors.forename: Suparna
authors.initials: S
authors.lastname: Qanungo
authors.forename: Michael T
authors.initials: MT
authors.lastname: Crow
authors.forename: Michiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Anna-Liisa
authors.initials: AL
authors.lastname: Nieminen
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051381:Rats
chemical_list: D051017:Apoptosis Regulatory Proteins; D009124:Muscle Proteins; C112246:NOL3 protein, human; C491788:Nol3 protein, rat