Analysis of expressed sequence tags from oil palm (Elaeis guineensis).
This is the first report of a systematic study of genes expressed by means of expressed sequence tag (EST) analysis in oil palm, a species of the Arecales order, a phylogenetically key clade of monocotyledons that is not widely represented in the sequence databases. Five different cDNA libraries were generated from male and female inflorescences, shoot apices and zygotic embryos and unidirectional systematic sequencing was performed. A total of 2411 valid EST sequences were thus obtained. Cluster analysis enabled the identification of 209 groups of related sequences and 1874 singletons. Putative functions were assigned to 1252 of the set of 2083 non-redundant ESTs obtained. The EST database described here is a first step towards gene discovery and cDNA array-based expression analysis in oil palm.
Jouannic
Stefan
S
IRD/CIRAD Oil Palm Laboratory, Centre IRD Montpellier, France. jouannic@mpl.ird.fr
Argout
Xavier
X
Lechauve
Frédéric
F
Fizames
Cécile
C
Borgel
Alain
A
Morcillo
Fabienne
F
Aberlenc-Bertossi
Frédérique
F
Duval
Yves
Y
Tregear
James
J
D028023:Arecaceae / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D018076:DNA, Complementary; D018744:DNA, Plant; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D015723:Gene Library; D008969:Molecular Sequence Data; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D018744:DNA, Plant