title: [Expression of survivin gene in pancreatic carcinoma].
abstract: OBJECTIVE
  To determine the expression status of survivin gene in pancreatic carcinoma.
  
  
  METHODS
  Expression of survivin gene was evaluated by immunohistochemistry, Western Blot and RT-PCR in 59 cases of pancreatic carcinoma along with their corresponding adjacent benign tissues, 11 cases of chronic pancreatitis, and 7 pancreatic carcinoma cell lines.
  
  
  RESULTS
  The positive expression rate of survivin in pancreatic carcinoma was 72.8% (43/59). There was no relationship between the expression of survivin and tumor stage and differentiation. No expression of survivin was detected in benign tissue adjacent to the tumors as well as in samples of chronic pancreatitis. All 7 pancreatic carcinoma cell lines showed a positive expression of survivin mRNA and protein.
  
  
  CONCLUSIONS
  The expression of survivin appears to be tumor specific to some extent in our pancreatic carcinoma samples. Survivin may be an ideal target for therapy against pancreatic carcinoma.
authors.affiliation: Department of Pathology, Peking Medical Union College Hospital, Beijing 100730, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liang
authors.forename: Tong-Hua
authors.initials: TH
authors.lastname: Liu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Jian
authors.initials: J
authors.lastname: Guan
authors.forename: Shuan-Zeng
authors.initials: SZ
authors.lastname: Wei
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Cao
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Quan-Cai
authors.initials: QC
authors.lastname: Cui
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin