title: Potential antifilarial activity of fruit extracts of Ficus racemosa Linn. against Setaria cervi in vitro.
abstract: Effect of alcoholic and aqueous extracts of the fruits of F. racemosa Linn., on the spontaneous movements of both the whole worm and nerve muscle preparation of Setaria cervi and on the survival of microfilariae in vitro was studied. Alcoholic as well as aqueous extracts caused inhibition of spontaneous motility of whole worm and nerve muscle preparation of Setaria cervi characterized by increase in amplitude and tone of contractions. Initial stimulatory effect was not observed with aqueous extract on whole worm preparation, while effect of alcoholic extract on whole worm and nerve muscle preparation was characterized by an increase in amplitude and tone of contractions followed by paralysis. The concentrations required to inhibit the movement of the whole worm and nerve muscle preparation for alcoholic extract of fruits of F. racemosa were 250 and 50 microg/ml, respectively, whereas aqueous extract caused inhibition of the whole worm and nerve muscle preparation at 350 and 150 microg/ml, respectively, suggesting a cuticular barrier. Both alcoholic and aqueous extracts caused death of microfilariae in vitro. LC50 and LC90 were 21 and 35 ng/ml, respectively for alcoholic, which were 27 and 42 ng/ml for aqueous extracts.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, India.
authors.forename: Vandna
authors.initials: V
authors.lastname: Mishra
authors.forename: Nizam U
authors.initials: NU
authors.lastname: Khan
authors.forename: K C
authors.initials: KC
authors.lastname: Singhal
mesh_terms: D000818:Animals; D000431:Ethanol; D030681:Ficus / Q000737:chemistry*; D005369:Filaricides / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D007928:Lethal Dose 50; D008842:Microfilariae / Q000187:drug effects; D009068:Movement / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D017182:Setaria Nematode / Q000187:drug effects*; D014867:Water
chemical_list: D005369:Filaricides; D010936:Plant Extracts; D014867:Water; D000431:Ethanol