[Isolation of the capsid protein gene of maize dwarf mosaic virus and its transformation in maize].
The MDMV (Maize Dwarf Mosaic Virus, MDMV) CP (Coat Protein, CP) gene was cloned by RT-PCR method and introduced into the embryonic calli derived from immature embryos of elite inbred 18-599hong and 18-599bai via particle bombardment. Bombarded calli were selected on selection medium containing 5-10 mg/L (PPT) Bialaphos. From resistant calli, 79 plantlets were regenerated. 18 of 79 were grown and harvested. The results of Southern blotting and PCR analysis demonstrated that MDMV CP have been integrated into the genome of the transgenic plants. PCR-positive progeny plants were artificially inoculated with MDMV strain B, and the average chlorosis of the functional leaves of each plant was investigated. The typical symptoms were observed from the leaves of the control inbreds. while, the presence of the MDMV CP gene provided resistance to inoculation with MDMV strain B.
Liu
Xiao-Hong
XH
Beijing Agro-biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China.
Zhang
Hong-Wei
HW
Liu
Xin
X
Liu
Xin-Jie
XJ
Tan
Zhen-Bo
ZB
Rong
Ting-Zhao
TZ
D036022:Capsid Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D009029:Mosaic Viruses / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified; D014162:Transfection; D003313:Zea mays / Q000235:genetics* / Q000821:virology*
D036022:Capsid Proteins