title: Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1.
abstract: The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.
authors.affiliation: Department of Molecular Biology, Umeå University, Sweden.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.forename: Lena
authors.initials: L
authors.lastname: Mogemark
authors.forename: Maria
authors.initials: M
authors.lastname: Fällman
mesh_terms: D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002460:Cell Line; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains / Q000235:genetics; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D001425:Bacterial Outer Membrane Proteins; C485512:Dbnl protein, mouse; C491468:Fyb protein, mouse; D008840:Microfilament Proteins; D011518:Proto-Oncogene Proteins; D019000:Phosphotyrosine; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn; D019061:src-Family Kinases; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia