title: Antifungal activity of Ocimum gratissimum towards dermatophytes.
abstract: The development of more effective and less toxic antifungal agents is required for the treatment of dermatophytosis. Plants and their preparations have been used as medicines against infectious diseases. Extracts of Ocimum gratissimum leaves were investigated for in vitro antifungal activity, using agar dilution technique against dermatophytes. The extracts (hexane, chloroform fractions, the essential oil and eugenol) produced antifungal activities against Microsporum canis, M. gypseum, Trichophyton rubrum and T. mentagrophytes. Trichophyton rubrum, the most common aetiological agent of dermatophytosis in Goiânia, state of Goiás, Brazil, was also the most susceptible dermatophyte. The hexane fraction and eugenol were the most active. Hexane fraction inhibited the growth of 100% of dermatophytes at a concentration of 125 microg ml(-1), while eugenol inhibited the growth of 80% of dermatophytes at this same concentration. These results show that extracts of O. gratissimum are active in vitro against human pathogenic dermatophytes.
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública da UFG, Goiânia, Goiás, Brazil. rosario@iptsp.ufg.br
authors.forename: M R R
authors.initials: MR
authors.lastname: Silva
authors.forename: J G
authors.initials: JG
authors.lastname: Oliveira
authors.forename: O F L
authors.initials: OF
authors.lastname: Fernandes
authors.forename: X S
authors.initials: XS
authors.lastname: Passos
authors.forename: C R
authors.initials: CR
authors.lastname: Costa
authors.forename: L K H
authors.initials: LK
authors.lastname: Souza
authors.forename: J A
authors.initials: JA
authors.lastname: Lemos
authors.forename: J R
authors.initials: JR
authors.lastname: Paula
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D001938:Brazil; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects; D031339:Ocimum / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts