Extractive spectrophotometric methods for the determination of oxomemazine hydrochloride in bulk and pharmaceutical formulations using bromocresol green, bromocresol purple and bromophenol blue.
Three simple, sensitive and accurate spectrophotometric methods have been developed for the determination of oxomemazine hydrochloride in bulk and pharmaceutical formulations. These methods are based on the formation of yellow ion-pair complexes between the examined drug and bromocresol green (BCG), bromocresol purple (BCP), and bromophenol blue (BPB) as sulphophthalein dyes in acetate-HCl buffer of pH 3.6, 3.4, and 4.0, respectively. The formed complexes were extracted with dichloromethane and measured at 405 nm for all three systems. The best conditions of the reactions were studied and optimized. Beer's law was obeyed in the concentration ranges 2.0-12, 2.0-13, and 2.0-14 microg mL(-1) with molar absorptivities of 3.2 x 10(4), 3.7 x 10(4), and 3.1 x 10(4) L mol(-1) cm(-1) for the BCG, BCP, and BPB methods, respectively. Sandell's sensitivity, correlation coefficient, detection and quantification limits are also calculated. The proposed methods have been applied successfully for the analysis of the drug in pure form and in its dosage forms. No interference was observed from common pharmaceutical excipients. Statistical comparison of the results with those obtained by HPLC method shows excellent agreement and indicates no significant difference in accuracy and precision.
El-Didamony
Akram M
AM
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt. ak_eldidamony@yahoo.com
D001961:Bromcresol Green; D001962:Bromcresol Purple; D001978:Bromphenol Blue; D002626:Chemistry, Pharmaceutical; D003499:Cyclic S-Oxides / Q000032:analysis*; D006633:Histamine Antagonists / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010640:Phenothiazines / Q000032:analysis*; D012997:Solvents
D003499:Cyclic S-Oxides; D006633:Histamine Antagonists; D007202:Indicators and Reagents; D010640:Phenothiazines; D012997:Solvents; D001978:Bromphenol Blue; D001962:Bromcresol Purple; C119593:oxomemazine; D001961:Bromcresol Green