The anti-endotoxic effect of o-aminobenzoic acid from Radix Isatidis.
OBJECTIVE
To study the anti-endotoxic effect of o-aminobenzoic acid (OABA) isolated from Banlangen(BLG).


METHODS
OABA was extracted and isolated from BLG and diluted into 0.5% solution. The concentration of endotoxin (ET) pretreated with OABA was quantitatively detected using Limulus test. The inhibition of ET-induced fever by OABA was measured in rabbits. The rates of lipopolysaccharides (LPS)-induced death in mice pretreated with or without OABA were then compared. The influence of OABA on the release of TNF-alpha and NO from macrophages induced by LPS was examined in mice.


RESULTS
After pretreatment with OABA, 84.4% of ET was destroyed. The ET-induced fever in rabbits decreased significantly and the rate of LPS-induced death in mice dropped from 70% to 20%. The release of TNF-alpha and NO induced by LPS in mice was inhibited dose-dependently when the concentration of OABA was between 0.125% and 0.5%.


CONCLUSIONS
OABA isolated from BLG has an anti-endotoxic effect.
Fang
Jian-guo
JG
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. fjg3560@sina.com
Liu
Yun-hai
YH
Wang
Wen-qing
WQ
Xie
Wei
W
Fang
Shu-xian
SX
Han
Hong-gang
HG
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D005260:Female; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D031219:Isatis / Q000737:chemistry*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D062367:ortho-Aminobenzoates; D009569:Nitric Oxide