Identification of glyoxalase-I as a protein marker in a mouse model of extremes in trait anxiety.
For >15 generations, CD1 mice have been selectively and bidirectionally bred for either high-anxiety-related behavior (HAB-M) or low-anxiety-related behavior (LAB-M) on the elevated plus-maze. Independent of gender, HAB-M were more anxious than LAB-M animals in a variety of additional tests, including those reflecting risk assessment behaviors and ultrasound vocalization, with unselected CD1 "normal" control (NAB-M) and cross-mated (CM-M) mice displaying intermediate behavioral scores in most cases. Furthermore, in both the forced-swim and tail-suspension tests, LAB-M animals showed lower scores of immobility than did HAB-M and NAB-M animals, indicative of a reduced depression-like behavior. Using proteomic and microarray analyses, glyoxalase-I was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. The same phenotype-dependent difference was found in red blood cells with NAB-M and CM-M animals showing intermediate expression profiles of glyoxalase-I. Additional studies will examine whether glyoxalase-I has an impact beyond that of a biomarker to predict the genetic predisposition to anxiety- and depression-like behavior.
Krömer
Simone A
SA
Max-Planck Institute of Psychiatry, D-80804 Munich, Germany.
Kessler
Melanie S
MS
Milfay
Dale
D
Birg
Isabel N
IN
Bunck
Mirjam
M
Czibere
Ludwig
L
Panhuysen
Markus
M
Pütz
Benno
B
Deussing
Jan M
JM
Holsboer
Florian
F
Landgraf
Rainer
R
Turck
Christoph W
CW
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D014151:Anti-Anxiety Agents / Q000008:administration & dosage; D001008:Anxiety Disorders / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001947:Breeding / Q000379:methods; D003975:Diazepam / Q000008:administration & dosage; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D005260:Female; D019417:Hindlimb Suspension / Q000502:physiology; D007791:Lactoylglutathione Lyase / Q000302:isolation & purification / Q000378:metabolism*; D008124:Locomotion / Q000235:genetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D046228:Microarray Analysis / Q000379:methods; D010641:Phenotype; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods; D011930:Reaction Time / Q000502:physiology; D015203:Reproducibility of Results; D012737:Sex Factors; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology; D013223:Statistics as Topic; D013550:Swimming; D013997:Time Factors; D014828:Vocalization, Animal / Q000187:drug effects / Q000502:physiology
D014151:Anti-Anxiety Agents; D015415:Biomarkers; D007791:Lactoylglutathione Lyase; D003975:Diazepam