title: Erythropoietin decreases cytotoxicity and nitric oxide formation induced by inflammatory stimuli in rat oligodendrocytes.
abstract: In the present study, we investigated whether erythropoietin (Epo) has a protective effect against cytotoxicity induced by interferon-gamma (IFN-gamma ) and lipopolysaccharide (LPS) in primary rat oligodendrocyte cultures. The possible modulatory effect of erythropoietin on inducible nitric oxide synthase (iNOS) mRNA expression and nitrite production were also analyzed. Erythropoietin exerted a significant protective effect against IFN-gamma and LPS-induced oligodendrocyte injury as determined by lactate dehydrogenase assay. Treatment with erythropoietin inhibited the expression of iNOS mRNA and nitrite production resulting from proinflammatory stimulation by IFN-gamma and LPS. These results suggest that erythropoietin has protective effects against inflammatory oligodendrocyte injury in vitro and may play a protective role in neurological disorders characterized by oligodendrocyte death, such as multiple sclerosis.
authors.affiliation: Learning Resources Center Research Laboratory, School of Medicine, Dokuz Eylul University, Inciralti, 35340, Izmir, Turkey. kkursadgenc@hotmail.com
authors.forename: K
authors.initials: K
authors.lastname: Genc
authors.forename: S
authors.initials: S
authors.lastname: Genc
authors.forename: H
authors.initials: H
authors.lastname: Baskin
authors.forename: I
authors.initials: I
authors.lastname: Semin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain; D002470:Cell Survival; D002478:Cells, Cultured; D004921:Erythropoietin / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007371:Interferon-gamma; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D012333:RNA, Messenger; C433788:lipopolysaccharide, E. coli O26-B6; D004921:Erythropoietin; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat