Characterization of a morphine-like molecule in secretory granules of chromaffin cells.
BACKGROUND
Morphine and morphine-like molecules were identified in numerous mammalian tissues. In particularly, morphine was present in adrenal gland and in PC-12 cells, corresponding to the cell line derived from rat adrenal medullary chromaffin cells.


METHODS
Morphine-like compounds were isolated after a deproteinization followed by a reverse phase high pressure chromatography purification coupled to a UV detector. This alkaloid was identified using nano-eletrospray ionization quadripole time of flight tandem mass spectrometry.


RESULTS
We demonstrate the presence of a morphine-like molecule inside the bovine chromaffin cell secretory granules. This alkaloid was also detected into the secretion medium of primary chromaffin cells in culture following nicotinic stimulation.


CONCLUSIONS
Our findings demonstrate that a morphine-like component is synthesized in chromaffin cells and secreted into the incubation medium upon stimulation. This finding strongly suggests that this material may be released from adrenal tissues in response to stressors.
Goumon
Yannick
Y
Inserm, 575 Physiopathology of the Nervous System, Strasbourg, France. goumon@neurochem.u-strasbg.fr
Strub
Jean-Marc
JM
Stefano
George B
GB
Van Dorsselaer
Alain
A
Aunis
Dominique
D
Metz-Boutigue
Marie-Hélène
MH
D000818:Animals; D002417:Cattle; D019439:Chromaffin Cells / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D009020:Morphine / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D022142:Secretory Vesicles / Q000378:metabolism*
D009020:Morphine