Molecularly imprinted solid-phase extraction of (-)-ephedrine from Chinese Ephedra.
Method of molecularly imprinted solid phase extraction (MISPE) of (-)-ephedrine from Chinese Ephedra has been developed in the research. The molecularly imprinted polymer (MIP) with good selectivity and affinity for (-)-ephedrine was synthesized with (-)-ephedrine as the template, methacrylic acid as the functional monomer. The washing and elution conditions in MISPE were selected and optimized for efficient analyte extraction and sample clean-up. A clean analytical HPLC base line of ephedra extract was obtained after MISPE, which indicated that the sample pre-treatment was efficient. Good recovery and precision were obtained in the assessment for the MISPE-HPLC procedure, which demonstrated it is a reliable method and can be used for the determination of (-)-ephedrine in herbal ephedra.
Dong
Xiangchao
X
College of Chemistry, Nankai University, Tianjin 300071, China. xcdong@nankai.edu.cn
Wang
Wei
W
Ma
Shujuan
S
Sun
Hui
H
Li
Yan
Y
Guo
Jingqiang
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D004809:Ephedrine