title: Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells.
abstract: Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, including bone morphogenetic protein (BMP), Wnt and FGF, are involved in heart development, scarce evidence is available about the exact signals that mediate cardiomyocyte differentiation. While investigating the involvement of BMP signaling in early heart formation in the mouse, we found that the BMP antagonist Noggin is transiently but strongly expressed in the heart-forming region during gastrulation and acts at the level of induction of mesendoderm to establish conditions conducive to cardiogenesis. We applied this finding to develop an effective protocol for obtaining cardiomyocytes from mouse ES cells by inhibition of BMP signaling.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Yuasa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Itabashi
authors.forename: Uichi
authors.initials: U
authors.lastname: Koshimizu
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Keijiro
authors.initials: K
authors.lastname: Sugimura
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Hattori
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Fukami
authors.forename: Takuya
authors.initials: T
authors.lastname: Shimazaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000008:administration & dosage / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008813:Mice, Inbred ICR; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D000099176:Noggin Protein
chemical_list: D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D000099176:Noggin Protein