[Induction and culture of hairy-root by Agrobacteriun rhizogenes in Dendrobium nobile].
OBJECTIVE
To obtain the hairy-roots of Dendrobium nobile.


METHODS
The hairy-root were obtained by infecting different explants with Agrobacteriun rhizogenes A4 and culture conditions of hariy root were optimized.


RESULTS
The best conditions of hairy root culture were 1/2MS medium with sucrose (3%) as carbon material and lactalbumin hydrolyze (1g/L) as nitron material.
Li
Fenghua
F
Biology Department, Zunyi Normal College, Zunyi 563002.
Tang
Shaohu
S
Sun
Min
M
D000470:Alkaloids / Q000096:biosynthesis; D003470:Culture Media; D031667:Dendrobium / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D012231:Rhizobium; D027343:Rhizome / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D046509:Tissue Culture Techniques / Q000379:methods
D000470:Alkaloids; D003470:Culture Media