title: Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas.
abstract: The DNA sequence of the gyrB gene is a suitable phylogenetic marker for bacterial systematics. In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach. PCR primers targeting the gyrB gene of aeromonads were designed and the resulting amplicons were analyzed by DGGE. The gyrB-DGGE analysis was evaluated with Aeromonas isolates and reference strains allowing discrimination of the majority of strains. The gyrB-DGGE analysis is a powerful tool to monitor the presence and evaluate the diversity of aeromonads in complex samples, as is the case of water from a wastewater treatment plant.
authors.affiliation: Center for Cell Biology, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Tacão
authors.forename: Alexandra
authors.initials: A
authors.lastname: Moura
authors.forename: Artur
authors.initials: A
authors.lastname: Alves
authors.forename: Isabel
authors.initials: I
authors.lastname: Henriques
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Saavedra
authors.forename: António
authors.initials: A
authors.lastname: Correia
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D020459:Genes, rRNA; D009691:Nucleic Acid Denaturation; D017686:Oncorhynchus mykiss / Q000382:microbiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase