Lipid peroxidation by "free" iron ions and myoglobin as affected by dietary antioxidants in simulated gastric fluids.
Grilled red turkey muscle (Doner Kabab) is a real "fast food" containing approximately 200 microM hydroperoxides, homogenized in simulated gastric fluid and oxidized more rapidly at pH 3.0 than at pH 5.0, after 180 min, producing 1200 and 600 microM hydroperoxides, respectively. The effects of "free" iron ions and metmyoglobin, two potential catalyzers of lipid peroxidation in muscle foods, were evaluated for linoleic acid peroxidation at pH 3.0 of simulated gastric fluid. The prooxidant effects of free iron ions on linoleic acid peroxidation in simulated gastric fluid was evaluated in the presence of ascorbic acid. At low concentrations of ascorbic acid, the effects were prooxidative, which was reversed at high concentrations. In the presence of metmyoglobin, ascorbic acid with or without free iron enhanced the antioxidative effect. Lipid peroxidation by an iron-ascorbic acid system was inhibited totally by 250-500 microM catechin at pH 3.0. The catechin antioxidant effect was determined also in the iron-ascorbic acid system containing metmyoglobin. In this system, catechin totally inhibited lipid peroxidation at a concentration 20-fold lower than without metmyoglobin. The ability of catechin to inhibit lipid peroxidation was also determined at a low pH with beta-carotene as a sensitive target molecule for oxidation. The results show that a significant protection was achieved only with almost 100-fold higher antioxidant concentration. Polyphenols from different groups were determined for the antioxidant activity at pH 3.0. The results show a high antioxidant activity of polyphenols with orthodihydroxylated groups at the B ring, unsaturation, and the presence of a 4-oxo group in the heterocyclic ring, as demonstrated by quercetin.
Lapidot
Tair
T
Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel.
Granit
Rina
R
Kanner
Joseph
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004032:Diet; D005419:Flavonoids / Q000494:pharmacology; D005750:Gastric Juice / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry; D009211:Myoglobin / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D014422:Turkeys; D014920:Wine / Q000032:analysis
D000975:Antioxidants; D005419:Flavonoids; D009211:Myoglobin; D010636:Phenols; D059808:Polyphenols; D006861:Hydrogen Peroxide; D007501:Iron