Imaging articular cartilage under compression--cartilage elastography.
We constructed a device to compress small samples of articular cartilage while the samples were imaged in a 1.5 T imager. With the use of a piezoelectric piston, the device compressed 1-cm-diameter cylindrical samples of articular cartilage (200 microm) at a rate of 2 Hz. Simultaneously, we imaged the samples with a displacement-sensitive stimulated-echo acquisition mode (STEAM) sequence. We validated the technique using tissue that mimicked silicone samples. We compared the results from the same cartilage samples before and after they were degraded by digestion in trypsin. The extent of degradation was visualized from T(1)-weighted images of the samples after they were soaked in 0.5 mmolar of GdDTPA. The resulting elastographic images show compression and differential strain in directions both parallel and perpendicular to the surface of the cartilage. The static elastographic images that depict compression made before digestion and after 5 and 15 hr of trypsin digestion show that the elastic modulus of the samples decreased with a spatial variation consistent with the enzymatic digestion as revealed by the T(1) images. We believe this technique will be useful in studies of the mechanical properties of articular cartilage and other tissues, and may in the future be extended to the clinical setting.
Hardy
Peter A
PA
Center for Biomedical Engineering, University of Kentucky, Lexington, 40536, USA. hardy@mri.uky.edu
Ridler
Anne C
AC
Chiarot
Cameron B
CB
Plewes
Don B
DB
Henkelman
R Mark
RM
D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology* / Q000378:metabolism; D002417:Cattle; D019245:Compressive Strength; D003287:Contrast Media; D004548:Elasticity; D005552:Forelimb; D019786:Gadolinium DTPA; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D014357:Trypsin
D003287:Contrast Media; D014357:Trypsin; D019786:Gadolinium DTPA