[Expression of S-100 positive dendritic cell, TIMP-1 and p63 and its significance in the primary laryngeal carcinoma].
OBJECTIVE
To evaluate the predictive role of S-100 positive dendritic cell, tissue inhibitor of metalloproteinases-1 (TIMP-1) and p63 gene in primary laryngeal carcinoma with epidemiology (smoking and drinking), histological grading, surgical treatment, TNM stage and prognosis by the tissuechip technology.


METHODS
We studied the expression of dendritic cell (S-100), TIMP-1 and p63 gene on a series of 85 primary laryngeal carcinoma patients who had ever received in our hospital between 1992 and 2000 by the tissuechip technology and SP method. The correlation of each score according to the intensity and percentage of labeled cells or intercellular substance with relevant clinical dada was statistically analyzed.


RESULTS
Some cases were lost or boasted no tumor tissue in our tissuechip. In available 79 patients, the rate of expressing S-100 positive dendritic cell is 59.5% (47/79), and the average percentage of its labeled cells in them is 8.71%. S-100 positive dentritic cells showed significant difference among different pathological grade group, early and late stage( P < 0.05). The rate is 55.7% (44/79) of the specimens whose basal membrane and extracellular matrix was strongly stained by TIMP-1; There was statistical significant in TIMP-1 protein demonstration between early and late stages, lymph node metastasis and 3-year survival rate ( P < 0.05) by chi-square test, but no relation with smoking, drinking, gender, age and histological classes (P > 0.05). There was wo statistical significant in p63 protein demonstration between TNM stages, lymph node metastasis, 3-year survival rate, smoking, drinking, gender, age and histological classes (P > 0.05).


CONCLUSIONS
The tissue microarray technique spent shorter time and less expense, and showed higher consistency in our essays. And the present study suggests TIMP-1 and S-100 could be the clinical discriminators in laryngeal carcinoma.
Dong
Pin
P
Department of Otolaryngology-Head and Neck Surgery, First People's Hospital of Shanghai Jiaotong University, Shanghai 200080, China. dongpin64@yahoo.com.cn
Li
Xiaoyan
X
Zhu
Zhenghua
Z
Yu
Ziwei
Z
Lu
Guangzhong
G
Sun
Zhenfeng
Z
Wang
Sang
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D009418:S100 Proteins / Q000032:analysis*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis
D009418:S100 Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D025521:Tumor Suppressor Proteins