title: Effects of heavy ion to the primary [correction of rimary] culture of mouse brain cells.
abstract: To investigate effects of low dose heavy particle radiation to CNS system, we adopted mouse neonatal brain cells in culture being exposed to heavy ions by HIMAC at NIRS and NSRL at BNL. The applied dose varied from 0.05 Gy up to 2.0 Gy. The subsequent biological effects were evaluated by an induction of apoptosis and neuron survival focusing on the dependencies of the animal strains, SCID, B6, B6C3F1, C3H, used for brain cell culture, SCID was the most sensitive and C3H the least sensitive to particle radiation as evaluated by 10% apoptotic criterion. The LET dependency was compared with using SCID and B6 cells exposing to different ions (H, C, Ne, Si, Ar, and Fe). Although no detectable LET dependency was observed in the high LET (55-200 keV/micrometers) and low dose (<0.5 Gy) regions. The survivability profiles of the neurons were different in the mouse strains and ions. In this report, a result of memory and learning function to adult mice after whole-body and brain local irradiation at carbon ion and iron ion.
authors.affiliation: International Space Radiation Laboratory, National Institute of Radiological Sciences (NIRS), Inage-ku, Chiba, Japan.
authors.forename: Kumie
authors.initials: K
authors.lastname: Nojima
authors.forename: Taeko
authors.initials: T
authors.lastname: Nakadai
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kohno
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Vazquez
authors.forename: Nakahiro
authors.initials: N
authors.lastname: Yasuda
authors.forename: Shunji
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001921:Brain / Q000528:radiation effects*; D002244:Carbon; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D020450:Heavy Ions; D018499:Linear Energy Transfer; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D009474:Neurons / Q000528:radiation effects; D010315:Particle Accelerators; D011829:Radiation Dosage; D012062:Relative Biological Effectiveness; D014965:X-Rays
chemical_list: D002244:Carbon