[Study on extraction process of Radix Bupleuri].
The orthogonal design was used to optimize extraction process of Radix Bupleuri with content of total saponin and yield of the extract as markers. Factors that have been chosen were ethanol concentration, ethanol consumption, extraction times and extraction time. Each factor had three levels. The result showed that the optimum extraction condition was 80% ethanol, 4 times the amount of material, refluxing for 4 times, 60 minutes each time. The optimized process was stable and workable.
Zhao
Lei
L
Gansu Dingxi Institute for Drug Control, Dingxi 743000.
Liu
Benliang
B
Wu
Fuxiang
F
Tao
Lanping
L
Liu
Jian
J
D000704:Analysis of Variance; D029972:Bupleurum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods; D013995:Time
D004365:Drugs, Chinese Herbal; D012503:Saponins; D000431:Ethanol