title: Activation of protein kinase C-delta attenuates kainate-induced cell death of cortical neurons.
abstract: We investigated the role of individual protein kinase C (PKC) isoforms during kainate toxicity in cortical neurons. Treatment with 50 microM kainate induced isoform-specific activation of PKC-delta according to the translocation from the soluble to the particulate fraction, while it caused remarkable decreases in PKC alpha, beta, epsilon and zeta in both fractions. Kainate-induced neuronal death was significantly increased by pharmacological inhibition of PKC-delta with rottlerin, suggesting a protective role of PKC-delta against kainate toxicity. A PKC activator phorbol 12-myristate 13-acetate remarkably attenuated the kainate-induced neuronal death. Although phorbol 12-myristate 13-acetate activates PKC-epsilon and PKC-delta, the protective effect of phorbol 12-myristate 13-acetate was almost completely abolished by rottlerin, but not by epsilonV1-2. These results suggest that activation of PKC-delta attenuates the kainate-induced cell death of cortical neurons.
authors.affiliation: Department of Physiology, School of Medicine, Ajou University, Suwon, Kyungkido 442-749, Korea. yisjung@ajou.ac.kr
authors.forename: Yi-Sook
authors.initials: YS
authors.lastname: Jung
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Lee
authors.forename: Hye-Seong
authors.initials: HS
authors.lastname: Park
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Shim
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Soo Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Baik
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Moon
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology; D007608:Kainic Acid / Q000633:toxicity; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051745:Protein Kinase C-delta; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D018690:Excitatory Amino Acid Agonists; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D013755:Tetradecanoylphorbol Acetate; D007608:Kainic Acid