[Integrative expression of XynB of Aspergillus niger UV-11 in industrial yeast].
The cDNA sequence of beta-xylanase gene (xynB) was cloned from Aspergillus niger UV-11. It was inserted into the yeast expression vector and the recombinant plasmid pAX2 was obtained. The plasmid pAX2 was introduced into an industrial S. cerevisiae 2.346 and integrated into yeast genome by co-transformation of a YEPtype plasmid pBEJ16 carrying G418 resistance. The stable engineered yeast strain XY2 was obtained. It could express and secret extracellular xylanase, and enhance the alcohol production in wheat flour fermentation compared with the host strain S. cerevisiae 2.346.
Li
Hai-yan
HY
State Key Laboratory of Microbio Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. lhyyy205@sohu.com
Zhu
Ling-xiang
LX
Mao
Ai-jun
AJ
Dong
Zhi-yang
ZY
D001234:Aspergillus niger / Q000201:enzymology*; D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000235:genetics; D005285:Fermentation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D043364:Endo-1,4-beta Xylanases