A specific primer pair for the diagnosis and identification of Acanthamoeba astronyxis by random amplified polymorphic DNA-polymerase chain reaction.
Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using the OPC20 primer (ACTTCGCCAC). The band specificity was confirmed by hybridization, using it as a probe, against all OPC20 amplifications from different Acanthamoeba species. Once the fragment was sequenced, we used it to design a specific primer pair that was useful for the identification of different isolates as A. astronyxis species.
Ortega-Rivas
A
A
Faculty of Farmacy, Department of Parasitology, University of La Laguna. Avda. Francisco Sánchez s/n, La Laguna CP 38271, Canary Islands, Spain. aortega@ull.es
Lorenzo-Morales
J
J
Martínez
E
E
Villa
M
M
Clavel
A
A
Valladares
B
B
del Castillo
A
A
D000048:Acanthamoeba / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015823:Acanthamoeba Keratitis / Q000175:diagnosis / Q000469:parasitology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D013045:Species Specificity; D014867:Water / Q000469:parasitology
D017931:DNA Primers; D016054:DNA, Protozoan; D014867:Water