title: [Substances for staining biological tissues: use of dyes in ophthalmology].
abstract: The azo dye trypan blue and the cyanine dye indocyanine green have been used in ophthalmology since the 1980 s to dye the anterior capsule in cataract operations and to stain epiretinal membranes in order to remove the membrana limitans interna (ILM). By means of a standardized in vitro test in accordance with DIN EN ISO 10993, it has now been shown that both dyes and the theoretically possible alternatives - the anthrachinone alizarincyanine green and the trityl dyes fast green and lissamine green - are cytotoxic in the normal concentrations of maximum 1.5 g/l. On the other hand, the new product Blueron(R) with the trityl dye patent blue, which has been developed to dye the anterior capsule, is completely biocompatible, even at a high dosage of up to 2.8 g/l.
authors.affiliation: Fluoron GmbH, Neu-Ulm. hiebl@fluoron.de
authors.forename: W
authors.initials: W
authors.lastname: Hiebl
authors.forename: B
authors.initials: B
authors.lastname: Günther
authors.forename: H
authors.initials: H
authors.lastname: Meinert
mesh_terms: D000880:Anthraquinones / Q000008:administration & dosage / Q000633:toxicity; D002387:Cataract Extraction; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D019773:Epiretinal Membrane / Q000473:pathology / Q000601:surgery; D006801:Humans; D066298:In Vitro Techniques; D007208:Indocyanine Green / Q000008:administration & dosage / Q000633:toxicity*; D008086:Lissamine Green Dyes / Q000008:administration & dosage / Q000633:toxicity; D008422:Materials Testing; D012163:Retinal Detachment / Q000473:pathology / Q000601:surgery; D012394:Rosaniline Dyes / Q000008:administration & dosage / Q000633:toxicity; D014343:Trypan Blue / Q000008:administration & dosage / Q000633:toxicity*; D018630:Vitreoretinopathy, Proliferative / Q000473:pathology / Q000601:surgery
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D008086:Lissamine Green Dyes; D012394:Rosaniline Dyes; C446400:D & C green 5; C024537:green 5; C035906:Fast Green; C008769:sulfan blue; D014343:Trypan Blue; D007208:Indocyanine Green