Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump.
MexJK requires OprM for erythromycin efflux but not for triclosan efflux. Deletion of 15 OprM family outer membrane proteins (OMPs) revealed that only the TolC homolog OpmH functions with MexJK for triclosan efflux. This is the first report of natural utilization of multiple OMPs by a given resistance nodulation cell division transporter/membrane fusion protein pair.
Chuanchuen
Rungtip
R
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Murata
Takeshi
T
Gotoh
Naomasa
N
Schweizer
Herbert P
HP
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004917:Erythromycin / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014260:Triclosan / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C501928:OpmH protein, Pseudomonas aeruginosa; C402263:OprM protein, Pseudomonas aeruginosa; D014260:Triclosan; D004917:Erythromycin