title: Extracellular hsp70 levels in children with septic shock.
abstract: OBJECTIVE
  To determine whether hsp70 is elevated in the plasma of children with septic shock.
  
  
  METHODS
  Cohort study.
  
  
  METHODS
  Two academic, tertiary pediatric intensive care units.
  
  
  METHODS
  Ninety-four children admitted to the pediatric intensive care unit with septic shock and 24 control children undergoing elective surgical procedures.
  
  
  METHODS
  Venous or arterial blood sampling.
  
  
  RESULTS
  Children admitted to the pediatric intensive care unit with a diagnosis of septic shock were enrolled in the study following written informed consent. The control group consisted of healthy children undergoing elective surgical procedures. Blood samples from children in the septic shock or control groups were obtained within 24 hrs of admission to the pediatric intensive care unit or during the preoperative visit. Samples were collected, centrifuged, and stored at -70 degrees C. The hsp70 levels were measured using a commercially available enzyme-linked immunosorbent assay. Results were analyzed by Wilcoxon's rank sum test. Extracellular hsp70 levels in children with septic shock were significantly elevated compared with control patients (51.6 ng/mL vs. 8.1 ng/mL, respectively, p = .0004).
  
  
  CONCLUSIONS
  Extracellular hsp70 levels are significantly elevated in children with septic shock compared with controls. Given the newly described cell signaling properties of hsp70, these data suggest that extracellular hsp70 may play a role in the host response during septic shock.
authors.affiliation: Department of Pediatrics, University of Cincinnati College of Medicine and Children's Hospital Research Foundation, Cincinnati, OH, USA.
authors.forename: Derek S
authors.initials: DS
authors.lastname: Wheeler
authors.forename: Lyle E
authors.initials: LE
authors.lastname: Fisher
authors.forename: John D
authors.initials: JD
authors.lastname: Catravas
authors.forename: Brian R
authors.initials: BR
authors.lastname: Jacobs
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Carcillo
authors.forename: Hector R
authors.initials: HR
authors.lastname: Wong
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012772:Shock, Septic / Q000097:blood*
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins