Molecular genetic analysis of QTLs for ferulic acid content in dried straw of rice (Oryza sativa L.).
Phenolic acids are secondary metabolic organic compounds produced by plants and often are mentioned as allelochemicals. This study was conducted to determine the genetic basis controlling the ferulic acid content of rice straw in a recombinant inbred (RI) population derived from a cross between a japonica variety, Asominori, with a higher content of ferulic acid, and an indica variety, IR24, with a lower content, using 289 RFLP markers. Continuous distributions and transgressive segregations of ferulic acid content were observed in the RI population, which showed that ferulic acid content in rice straw was quantitatively inherited. Single marker analysis and composite interval mapping identified three quantitative trait loci (QTLs) for ferulic acid content with LOD values of 2.03 (chromosome 3), 3.16 (chromosome 6), and 3.06 (chromosome 7); all three had increased additive effects (13.5, 18.3, and 18.1 microg g(-1)) from the Asominori parent and accounted for 5.5, 16.9, and 12.8% of total phenotypic variation, respectively. This is the first report on the identification of QTLs associated with ferulic acid and their chromosomal localization on the molecular map of rice. The tightly linked molecular markers that flank the QTLs might be useful in breeding and selection of varieties with higher phenolic acid content.
Dong
Yanjun
Y
Agricultural Faculty, Miyazaki University, Miyazaki City, 889-2192, Japan. dong@cc.miyazaki-u.ac.jp
Tsuzuki
E
E
Kamiunten
H
H
Lin
Dongzhi
D
Terao
H
H
Matsuo
M
M
Cheng
Shihua
S
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003373:Coumaric Acids / Q000032:analysis*; D003433:Crosses, Genetic; D012275:Oryza / Q000737:chemistry* / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D040641:Quantitative Trait Loci
D003373:Coumaric Acids; C004999:ferulic acid