Neuroinflammatory responses from microglia recovered from HIV-1-infected and seronegative subjects.
Microglial and macrophage infection and immune activation underlie the pathogenesis of HIV-1-associated dementia (HAD). To assess microglial function in HAD, we isolated cells from brain tissues recovered from an HIV-1-infected patient within 4 h of death. Brain tissue from seronegative patients served as controls. Regional neuropathology was correlated to microglial function. HIV-1-patient microglia formed multinucleated giant cells and produced progeny virions. These microglia secreted reduced basal and LPS-stimulated TNF-alpha levels compared to controls. Monocytes from seronegative donors paralleled these diminished immune responses following repeated LPS-activation. These results demonstrate changes in innate microglial function following viral infection or chronic immune activation.
Ghorpade
Anuja
A
Laboratory of Cellular Neuroimmunology, 985215 Nebraska Medical Center, Omaha, NE, 68198-5215, USA. aghorpad@unmc.edu
Persidsky
Yury
Y
Swindells
Susan
S
Borgmann
Kathleen
K
Persidsky
Raisa
R
Holter
Spring
S
Cotter
Robin
R
Gendelman
Howard E
HE
D000328:Adult; D001921:Brain / Q000276:immunology / Q000473:pathology / Q000821:virology; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology*; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000473:pathology / Q000821:virology; D008297:Male; D017628:Microglia / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D008875:Middle Aged