title: Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum beta-lactamases in Spain.
abstract: Clonal dissemination of extended-spectrum beta-lactamases (ESBL) in 170 Escherichia coli isolates and 70 Klebsiella pneumoniae isolates from a nationwide study of 40 Spanish centers in 2000 was not observed in most centers. The most prevalent ESBL were CTX-M-9 (27.3%), SHV-12 (23.9%), and CTX-M-14 (20.5%) for E. coli and TEM-3 (16.7%) and TEM-4 (25%) for K. pneumoniae. A new ESBL, TEM-133, with mutations L21F, E104K, and R164S, was identified.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Apdo. 914, Seville 41080, Spain. joserhb@us.es
authors.forename: J R
authors.initials: JR
authors.lastname: Hernández
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Martínez
authors.forename: R
authors.initials: R
authors.lastname: Cantón
authors.forename: T M
authors.initials: TM
authors.lastname: Coque
authors.forename: A
authors.initials: A
authors.lastname: Pascual
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D001618:beta-Lactamases