Technical improvement of human pancreatic islet isolation.
BACKGROUND
A key factor for successful islet isolation is to place the optimal amount of enzyme into the pancreatic ducts prior to starting digestion of pancreatic glands. To improve this procedure, we introduced novel techniques to identify and repair tissue damage resulting in leakage of collagenase solution.


METHODS
One hundred twelve standardized consecutive islet isolations were for the effects of dye and glue on islet yield, islet function using a perifusion assay, and the possibility of clinical transplantation. One group of pancreata (n = 26) obtained en bloc together with duodenum were carefully detached with ligation of accessory ducts in an isolation unit (WPD group), whereas the pancreata were dissected from the duodenum in the operating room in the other 86 isolations. In 28 of 86 isolations, whole glands were used (WP group), while only the body and tail area were applied in the remaining 58 isolations (PP group).


RESULTS
Both dye and glue effectively prevented leakage of collagenase from the gland. Both islet yield and success rate were higher with these tools without adverse effects on islet function or collagenase activity. The success rate of isolations and islet yield were significantly higher in the WPD group (P = .02 and .001, respectively).


CONCLUSIONS
Dye and glue may be useful tools to improve human islet isolation procedures. In addition, the use of the whole pancreas further improves the outcome.
Goto
M
M
Department of Radiology, Oncology and Clinical Immunology, Division of Clinical Immunology, the Rudbeck Laboratory, University Hospital, Uppsala, Sweden. gotokichi@aol.com
Eich
T M
TM
Stahle
M
M
Malmborg
A
A
Engkvist
M
M
Korsgren
O
O
D000269:Adhesives; D002469:Cell Separation / Q000379:methods; D004396:Coloring Agents; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology; D010179:Pancreas / Q000166:cytology* / Q000502:physiology; D010180:Pancreatectomy
D000269:Adhesives; D004396:Coloring Agents