title: [Construction and bio-activity of the chimeric protein of BMP2-EGFP].
abstract: OBJECTIVE
  To construct the recombined retroviral expression vector of BMP2/pLEGFP and investigate the bio-activity of the expressed chimeric protein.
  
  
  METHODS
  The recombinant vector constructed by gene recombinant technology was analyzed by restriction enzyme digestion and PCR. BMP2/pLEGFP was transfected into COS-7 cells with liposome transfection reagents for transient expression. The expression of chimeric protein BMP2/EGFP was identified by fluorescent microscope and Western blotting. The bio-activity was examined by the cellular activity and animal heterotopic osteogenesis experiment.
  
  
  RESULTS
  The recombinant plasmid proved successful by restriction enzyme digestion and PCR. The expression of the chimeric protein was shown by fluorescent microscope and Western blotting. The chimeric protein had the double bio-activities of BMP2 and EGFP identified by the cellular activity and animal heterotopic osteogenesis tests.
  
  
  CONCLUSIONS
  The recombinant vector of BMP2/pLEGFP is successfully constructed by the gene recombinant technology and its chimeric protein has double bioactivities of BMP2 and EGFP.
authors.affiliation: Institute of Endemic Disease, Key Laboratory of Environment and Genes Related to Diseases, Education Ministry, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yin-gang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Xiong
authors.initials: X
authors.lastname: Guo
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Shi
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zou
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D019556:COS Cells; D002522:Chlorocebus aethiops; D005057:Eukaryotic Cells / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta; D049452:Green Fluorescent Proteins