[Construction of Bacillus thuringiensis labeled recombinant strain and horizontal transfer of its cry1Ac10 gene].
A recombinant plasmid pBMBZGC10 was obtained by the ligation of gfp-cry1Ac10 fusion gene and vector plasmid pAD4412, which was then introduced by gene pulser into acrystalliferous strain CryB, and a recombinant strain CryB(pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spraying on Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum leaves. The results of fluorescent detection and PCR amplification revealed that cry1Ac10 gene did not transfer into indigenous bacteria, actinomyces and fungi in test soil, and could not be detected in roots, stems and leaves of test plants.
Zhou
Qin
Q
State Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. zhouqin@mail.hzau.edu.cn
Sun
Ming
M
Li
Lin
L
Yang
Zaiqing
Z
Yu
Ziniu
Z
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D049452:Green Fluorescent Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D010572:Pest Control, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D049452:Green Fluorescent Proteins