Inhibitory effects of isorhamnetin-3-O-beta-D-glucoside from Salicornia herbacea on rat lens aldose reductase and sorbitol accumulation in streptozotocin-induced diabetic rat tissues.
The inhibitory effects of compounds from Salicornia herbacea (Chenopodiaceae) on rat lens aldose reductase (RLAR) and sorbitol accumulation in streptozotocin-induced diabetic rat tissues were investigated. The various fractions from the MeOH extract of S. herbacea were tested for their effects on RLAR in vitro. Among them, the EtOAc fraction was found to exhibit a potent RLAR inhibition (IC(50)=0.75 microg/ml), from which an active principle as a potent AR inhibitor was isolated and its chemical structure was elucidated as isorhamnetin-3-O-beta-D-glucoside (1) by spectral analysis. Compound 1 exhibited a potent RLAR inhibition in vitro, its IC(50) being 1.4 microM. Compound 1, when administered orally at 25 mg/kg in streptozotocin (STZ)-induced diabetic rats, caused not only a significant inhibition of serum glucose concentration but also sorbitol accumulation in the lenses, red blood cells (RBC), and sciatic nerves. These results indicate that compound 1 from S. herbacea is a leading compound for further study as a new drug for the prevention and/or treatment of diabetes and its complications.
Lee
Yeon Sil
YS
Seokwon Life Science Research Institute, World Sea Green Co. Ltd., Paju 413-832, Korea.
Lee
Sanghyun
S
Lee
Hye Seung
HS
Kim
Bak-Kwang
BK
Ohuchi
Kazuo
K
Shin
Kuk Hyun
KH
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D019663:Chenopodiaceae; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D011794:Quercetin / Q000031:analogs & derivatives; D051381:Rats; D017207:Rats, Sprague-Dawley; D013012:Sorbitol / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology
D004791:Enzyme Inhibitors; D044948:Flavonols; D005960:Glucosides; D010936:Plant Extracts; C047368:3-methylquercetin; D013012:Sorbitol; D011794:Quercetin; D000449:Aldehyde Reductase