title: Collagenase-3 (MMP-13) is expressed in periapical lesions: an immunohistochemical study.
abstract: OBJECTIVE
  To determine whether or not matrix metalloproteinase 13 (MMP-13) is present in periapical granulomas with and without epithelium.
  
  
  METHODS
  Seventeen open periapical granulomas of pulpal origin (seven lesions without epithelium and 10 with proliferating epithelium) were fixed in formalin and then embedded in paraffin prior to being processed for immunohistochemical analysis. A monoclonal antibody against human MMP-13 was used to evaluate MMP-13 expression. Immunocomplexes were subsequently treated with the secondary antibody and then detected by means of streptavidin peroxidase. Immunoreactivity was visualized by development with 3,3'-diaminobenzidine.
  
  
  RESULTS
  An immunopositive cytoplasmatic reaction for MMP-13 was observed in all the specimens, although the immunostaining by anti-MMP-13 antibody was heterogeneous and its levels varied according to histopathological findings. In periapical lesions without epithelium MMP-13 immunolabelling was detected in a few fibroblast-like cells, and in some plasma cells within the granulomatous tissue. A clear upregulation of MMP-13 expression was detected in periapical lesions with epithelium, especially in small island and thin strands of epithelium.
  
  
  CONCLUSIONS
  The expression pattern of MMP-13 demonstrates that it is involved in the conversion of a periapical granuloma with epithelium into a radicular cyst. This property is related to the ability of MMP-13 to influence not only the migration of epithelial cell but also the invasion of granulomatous tissue.
authors.affiliation: Department of Medical and Surgical Science, II Dental Unit, University of Catania, Catania, Italy. rleonard@unict.it
authors.forename: R
authors.initials: R
authors.lastname: Leonardi
authors.forename: R
authors.initials: R
authors.lastname: Caltabiano
authors.forename: C
authors.initials: C
authors.lastname: Loreto
mesh_terms: D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D017364:Collagenases / Q000032:analysis*; D003593:Cytoplasm / Q000201:enzymology; D004847:Epithelial Cells / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13; D010484:Periapical Granuloma / Q000201:enzymology*; D010486:Periapical Tissue / Q000201:enzymology; D010950:Plasma Cells / Q000201:enzymology; D011842:Radicular Cyst / Q000201:enzymology; D015854:Up-Regulation
chemical_list: D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13