[The relationship of the expression of proliferetron--related antigens Ki67 and PCNA in the cells of ductal breast cancer with the differentiation grade].
OBJECTIVE
Ki67 and PCNA proteins are characteristic for cells in mitotic cycle and represent unfavourable prognostic indices. Intensity of the proteins expression directly correlates with grade of tumour differentiation. They belong to the second category of prognostic factors in breast cancer according to the College of American Pathologists.


METHODS
Present study aimed at examining the relationship between intensity of expression of proliferation-related antigens--Ki67 and PCNA in cells of ductal breast cancer of G1, G2 or G3 differentiation grade.


METHODS
In paraffin sections of 60 ductal breast cancers, immunocytochemical reactions were performed to detect expression of Ki67 and PCNA. Following semiquantitative appraisal of studied preparations, appropriate statistical tests were used to document statistical relationships.


RESULTS
Intensity of Ki67 expression was most pronounced in G3 group and least pronounced in G1 group (ANOVA rang Kruskal-Wallis, p=0.00001). In the case of PCNA we did not show any differences. Only in the G1 we showed a positive correlation between expression intensity of studied antigens (Spearmans rang correlation, r=0.67, p=0.024).


CONCLUSIONS
The study showed that Ki67 and not PCNA correlates with morphologic features of proliferation.
Surowiak
Paweł
P
Katedra i Zakład Histologii i Embriologii, Akademia Medyczna we Wrocławiu.
Pudełko
Marek
M
Maciejczyk
Adam
A
Dziegiel
Piotr
P
Wojnar
Andrzej
A
Zabel
Maciej
M
D000704:Analysis of Variance; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008940:Mitotic Index; D009367:Neoplasm Staging; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen