title: Preparation and characterization of a monoclonal antibody specific to histone acetyltransferase from Plasmodium falciparum.
abstract: We have obtained a specific monoclonal antibody (MAb) against the Plasmodium falciparum histone acetyl transferase (PfGcn5), a transcriptional factor that possesses HAT activity directed to the amino terminal of histone H3. To prepare this antibody, a 968-base pair (bp) DNA fragment of PfGcn5 gene corresponding to C-terminal domain was obtained by RT-PCR and cloned into prokaryotic expression vector pGEX-4T3. MAb against PfGcn5 was obtained with hybridoma technique and ELISA screening using either purified GSTPfGcn5 protein or purified GST protein alone as a control. One MAb, named Pf.r2, was able to identify the PfGcn5 protein in nuclear extract from P. falciparum and immunofluorescence assays. This MAb will be a helpful tool to perform a variety of assays to identify the other components of PfGcn5 complexes.
authors.affiliation: Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del IPN, D.F., México.
authors.forename: Dvorak
authors.initials: D
authors.lastname: Montiel-Condado
authors.forename: Héctor
authors.initials: H
authors.lastname: Romero-Ramírez
authors.forename: Carmen
authors.initials: C
authors.lastname: Ramírez-Estudillo
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Santos-Argumedo
authors.forename: Rosaura
authors.initials: R
authors.lastname: Hernández-Rivas
mesh_terms: D000123:Acetyltransferases / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D051548:Histone Acetyltransferases; D006801:Humans; D006825:Hybridomas; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
chemical_list: D000911:Antibodies, Monoclonal; D000123:Acetyltransferases; D051548:Histone Acetyltransferases