The Sca-1 cell surface marker enriches for a prostate-regenerating cell subpopulation that can initiate prostate tumorigenesis.
Sca-1 (stem cell antigen-1) enriches for murine prostate cells capable of regenerating tubular structures containing basal and luminal cell lineages in a dissociated cell prostate regeneration system. Sca-1(+) fractions are enriched for cells at the G(0) stage of the cell cycle, and Sca-1(+) cells cluster in the proximal region of prostatic tubules where replication-quiescent cells have been localized. Castration-induced enrichment for androgen-independent cells results in a concomitant enrichment for Sca-1(+) cells. Genetic perturbations of PTEN/AKT signaling in prostate-regenerating cells leads to the initiation of tumorigenesis, and cancer progression is associated with a dramatic increase in Sca-1(+) cells. Sca-1-enriched prostate-regenerating cells possess multiple stem/progenitor cell properties and can serve as targets for cancer initiation.
Xin
Li
L
Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, and Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.
Lawson
Devon A
DA
Witte
Owen N
ON
D000199:Actins / Q000378:metabolism; D000728:Androgens / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D018450:Disease Progression; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D007150:Immunohistochemistry; D008280:Magnetics; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000473:pathology; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism; D012038:Regeneration; D015398:Signal Transduction
D000199:Actins; D000728:Androgens; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C504399:Psca protein, mouse; D011944:Receptors, Androgen