Progesterone receptor subtypes in vascular smooth muscle cells of human aorta.
Progesterone is involved in various functions of the cardiovascular system, including those of vascular smooth muscle cells (VSMCs) via progesterone receptor (PR). Progesterone has also been postulated to be involved in inhibition of VSMC proliferation via PR. However, the details of PR expression have remained largely unknown in human cardiovascular VSMCs. Therefore, we first examined the relative levels of PR isoform (PR-A and PR-B) expression in VSMCs, using both immunohistochemistry and quantitative RT-PCR analysis. PR-B was equally expressed between male and female aorta, but PR-A was more abundant in female than in male aorta. This finding demonstrated that the status of PR subtype expression was associated with the difference of genders.
Nakamura
Yasuhiro
Y
Department of Pathology, Tohoku University School of Medicine, Seiryomachi, Sendai, Japan.
Suzuki
Takashi
T
Inoue
Tsukasa
T
Tazawa
Chika
C
Ono
Katsuhiko
K
Moriya
Takuya
T
Saito
Haruo
H
Ishibashi
Tadashi
T
Takahashi
Shoki
S
Yamada
Shogo
S
Sasano
Hironobu
H
D000328:Adult; D000368:Aged; D001012:Aorta, Abdominal / Q000737:chemistry*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D032389:Myocytes, Smooth Muscle / Q000737:chemistry*; D017698:Postmenopause; D017697:Premenopause; D012333:RNA, Messenger / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
D012333:RNA, Messenger; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B