Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis.
Adipocytes arise from multipotent stem cells of mesodermal origin, which also give rise to the muscle, bone, and cartilage lineages. However, signals and early molecular events that commit multipotent stem cells into the adipocyte lineage are not well established mainly due to lack of an adequate model system. We have identified a novel source of adult stem cells from the external murine ears referred to here as an ear mesenchymal stem cells (EMSC). EMSC have been isolated from several standard and mutant strains of mice. They are self-renewing, clonogenic, and multipotent, since they give rise to osteocytes, chondrocytes, and adipocytes. The in vitro characterization of EMSC indicates very facile adipogenic differentiation. Morphological, histochemical, and molecular analysis after the induction of differentiation showed that EMSC maintain adipogenic potentials up to fifth passage. A comparison of EMSC to the stromal-vascular (S-V) fraction of fat depots, under identical culture conditions (isobutyl-methylxanthine, dexamethasone, and insulin), revealed much more robust and consistent adipogenesis in EMSC than in the S-V fraction. In summary, we show that EMSC can provide a novel, easily obtainable, primary culture model for the study of adipogenesis.
Rim
Jong-Seop
JS
Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
Mynatt
Randall L
RL
Gawronska-Kozak
Barbara
B
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D020219:Chondrogenesis; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004431:Ear, External / Q000166:cytology*; D005947:Glucose / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D008071:Lipoprotein Lipase; D005947:Glucose