title: Effects of dexamethasone on TNF-alpha-induced release of cytokines from purified human blood eosinophils.
abstract: BACKGROUND
  TNF-alpha is an important mediator in allergy also for its effects on eosinophils.
  
  
  METHODS
  The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated.
  
  
  RESULTS
  The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils.
  
  
  CONCLUSIONS
  These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that in turn is important for the perpetuation of the allergic inflammation.
authors.affiliation: Cell Biology Unit, Glaxo Wellcome SKB, Gunnels Wood Stevenage, Herts, SG1 2NY, UK.
authors.forename: Iain
authors.initials: I
authors.lastname: Uings
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Puxeddu
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Temkin
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Smith
authors.affiliation: Cell Biology Unit, Glaxo Wellcome SKB, Gunnels Wood Stevenage, Herts, SG1 2NY, UK.
authors.forename: Dilniya
authors.initials: D
authors.lastname: Fattah
authors.affiliation: Cell Biology Unit, Glaxo Wellcome SKB, Gunnels Wood Stevenage, Herts, SG1 2NY, UK.
authors.forename: Keith P
authors.initials: KP
authors.lastname: Ray
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Francesca
authors.initials: F
authors.lastname: Levi-Schaffer