Generation of a mouse monoclonal antibody against chicken interleukin-6.
A monoclonal antibody (MAb) specific for chicken interleukin-6 (chIL-6) was generated by using Balb/c mice immunized with recombinant chIL-6 (rchIL-6). On Western blot analysis, the MAb, designated E3, reacted with rchIL-6 but not with recombinant murine IL-6 (rmIL-6). The MAb E3 also reacted with supernatant of the chicken macrophage-like cell line HD11 stimulated with lipopolysaccaride. The rchIL-6-induced phosphorylation of STAT3 in a chicken hybridoma cell line was inhibited by addition of MAb E3 in a dose-dependent manner. These results indicate that the MAb E3 specific for chIL-6 is useful for detection and functional analysis of chIL-6.
Nishimichi
Norihisa
N
Laboratory of Immunobiology, Department of Molecular and Applied Biological Science, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Aosasa
Masayoshi
M
Kawashima
Tsuyoshi
T
Horiuchi
Hiroyuki
H
Furusawa
Shuichi
S
Matsuda
Haruo
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D006825:Hybridomas; D015850:Interleukin-6 / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D015850:Interleukin-6