[Study of mechanism of differentiation of bone stromal stem cells into neurons in vitro].
OBJECTIVE
To explore the mechanism of differentiation of bone marrow stromal cells (BMSCs) into neurons in different micro-environments in vitro.


METHODS
BMSCs were isolated from bone marrow of SD rats and cultured and expanded in vitro. After being identified by immunofluorescence staining, the BMSCs labeled with PKH67 were co-cultured with foetal brain neural cells in the same plate well or in two-layer Petri dish. 8 days later, the BMSCs were detected by immunofluorescence staining.


RESULTS
After being co-cultured with foetal brain neural cells at the same time, some BMSCs differentiated into neurons. (32.72+/-2.56)% of the BMSCs expressed neuron-specific enolase (NSE) in the co-cultured group, which was obviously much more than that in control group (P <0.05). Only (4.87+/-0.79)% of the BMSCs expressed NSE when the BMSCs co-cultured with foetal brain neural cells in two-layer Petri dish, which had no difference with the control group (P>0.05). The number of differentiated BMSCs was less than that of the co-cultured group (P <0.05).


CONCLUSIONS
In vitro, the local microenvironment formed by neural cells can promote BMSCs to differentiate into neurons, and close contact between BMSCs and neural cells is an important condition that induce BMSC to differentiate into neurons.
Yan
Bin-bin
BB
Department of Physiology, Harbin Medical University, Harbin 150086, China. ybbxuz@163.com
Wang
Li-li
LL
Qiao
Jun-feng
JF
Peng
Hai-sheng
HS
Cao
Jing-yan
JY
Li
Hu-lun
HL
Jin
Lian-hong
LH
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D018920:Coculture Techniques; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*
D010751:Phosphopyruvate Hydratase