title: Scallop DMT functions as a Ca2+ transporter.
abstract: We identified a DMT (divalent metal transporter) homologous protein that functions as a Ca(2+) transporter. Scallop DMT cDNA encodes a 539-amino-acid protein with 12 putative membrane-spanning domains and has a consensus transport motif in the fourth extracellular loop. Since its mRNA is significantly expressed in the gill and intestine, it is assumed that scallop DMT transports Ca(2+) from seawater by the gill and from food by the intestine. Scallop DMT lacks the iron-responsive element commonly found in iron-regulatory proteins, suggesting that it is free of the post-transcriptional regulation from intracellular Fe(2+) concentration. Scallop DMT distinctly functions as a Ca(2+) transporter unlike other DMTs, however, it also transports Fe(2+) and Cd(2+) similar to them.
authors.affiliation: Laboratory of Marine Biological Function, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan. toyohara@kais.kyoro-u.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Toyohara
authors.forename: Sayuri
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Masatomi
authors.initials: M
authors.lastname: Hosoi
authors.forename: Masaya
authors.initials: M
authors.lastname: Takagi
authors.forename: Isao
authors.initials: I
authors.lastname: Hayashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakao
authors.forename: Shuji
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D007501:Iron / Q000378:metabolism; D008845:Microinjections; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014981:Xenopus
chemical_list: D027682:Cation Transport Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D002104:Cadmium; D007501:Iron; D002118:Calcium