title: Development and use of an internal positive control for detection of Bordetella pertussis by PCR.
abstract: An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.
authors.affiliation: Laboratoire de Bactériologie-Hygiène, Hôpital A. Calmette, F-59037-Lille Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Herwegh
authors.forename: Christophe
authors.initials: C
authors.lastname: Carnoy
authors.forename: Frédéric
authors.initials: F
authors.lastname: Wallet
authors.forename: Caroline
authors.initials: C
authors.lastname: Loïez
authors.forename: René J
authors.initials: RJ
authors.lastname: Courcol
mesh_terms: D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial