Cloning and characterization of a novel human homolog* of mouse U26, a putative PQQ-dependent AAS dehydrogenase.
Mouse U26 has been defined as a 2-aminoadipic 6-semialdehyde dehydrogenase. It was speculated to be a PQQ-dependent AAS dehydrogenase due to the research of demonstrating PQQ as a new B vitamin. We isolated a novel human cDNA from the human fetal brain cDNA library we constructed. Its deduced protein was most related to mouse U26. Thus, we termed it human U26. This putative protein contains an AMP-binding domain, a Phosphopantetheine-binding domain and six PQQ-binding motifs. Human U26 mRNA is ubiquitously expressed in adult tissues and is highly expressed in colon adenocarcinoma (CX-1) and colon adenocarcinoma (GI-112) cell lines. Further study should be made to clarify the precise function of human U26.
Wang
Liu
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China.
Jil
Chaoneng
C
Xu
Yiren
Y
Xu
Jian
J
Dai
Jianfeng
J
Wu
Qihan
Q
Wu
Maoqing
M
Zou
Xianqiong
X
Sun
Liyun
L
Gu
Shaohua
S
Xie
Yi
Y
Mao
Yumin
Y
D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D045542:PQQ Cofactor / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014018:Tissue Distribution
D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; C473747:U26 protein, mouse; D045542:PQQ Cofactor; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; C503677:AASDH protein, human; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase