[Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors].
Fluorescence in situ hybridization (FISH) was used to compare aneuploidy rates in four autosomes and two sex chromosomes in interphase nuclei of noncultivated (quiescent) and cultivated (induced to divide with phytohemagglutinin (PHA)) leukocytes in people engaged in nuclear-chemical industry and in a control group of people not exposed to mutagenic factors occupationally or at home. The overall rates of numerical chromosome aberrations for all of the six chromosomes studied showed little difference, although a higher rate of loss of the X- and Y-chromosomes was observed in the exposed group. In individuals exposed to several adverse environmental factors, the overall rate of numerical chromosome aberrations in cultivated cells after at least one DNA replication cycle exceeded that in noncultivated cells by 52% (P = 0.01), whereas only a trend for its increase was observed in the control group (23%, P = 0.25). Thus, the effect of adverse environmental factors in humans caused more than a twofold increase in the difference between the rates of aneuploid cells in cultivated and non-cultivated leukocytes in the exposed group as compared to control. It is conjectured that cell division is accompanied by the expression of potential damage of mitotic chromatid segregation apparatus accumulated in vivo. These defects, realized during cell division, bring about numerical chromosome aberrations.
Nazarenko
S A
SA
Research Institute of Medical Genetics, Tomsk Research Center, Russian Academy of Medical Sciences, Tomsk, 634050 Russia. snaz@img.tsu.ru
Timoshevsky
V A
VA
D000328:Adult; D000782:Aneuploidy; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects