title: Purification and characterization of human caseinomacropeptide produced by a recombinant Saccharomyces cerevisiae.
abstract: Caseinomacropeptide (CMP) is a biologically active polypeptide derived from the C-terminal of milk kappa-casein. CMP is heterogeneous since it is modified differently by glycosylation and phosphorylation after translation. Recently, recombinant human CMP (hCMP) has been produced as a secretory product in yeast. The present study aimed at the purification and characterization of recombinant hCMP. By sequential molecular cut-off ultrafiltration and anion-exchange chromatography, the recombinant hCMP in the culture broth could be purified to an HPLC purity over 94%. The authenticity of the purified hCMP was confirmed by sequence analysis of N-terminal amino acids. The recombinant hCMP was estimated to be 7.0kDa by SDS-PAGE, and showed a lower glycosylation than the natural bovine CMP.
authors.affiliation: Department of Chemical Engineering, Institute for Environmental Technology and Industry, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
authors.forename: Whankoo
authors.initials: W
authors.lastname: Kang
authors.forename: Hee Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
mesh_terms: D000818:Animals; D002364:Caseins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D006801:Humans; D030781:Organisms, Genetically Modified; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C065989:caseinomacropeptide