title: Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana.
abstract: Epithiospecifier protein (ESP) is a protein that catalyses formation of epithionitriles during glucosinolate hydrolysis. In vitro assays with a recombinant ESP showed that the formation of epithionitriles from alkenylglucosinolates is ESP and ferrous ion dependent. Nitrile formation in vitro however does not require ESP but only the presence of Fe(II) and myrosinase. Ectopic expression of ESP in Arabidopsis thaliana Col-5 under control of the strong viral CaMV 35S promoter altered the glucosinolate product profile from isothiocyanates towards the corresponding nitriles.
authors.affiliation: Department of Agricultural Sciences, Imperial College, University of London, Wye, Ashford, Kent TN25 5AH, UK.
authors.forename: Marta de Torres
authors.initials: Mde T
authors.lastname: Zabala
authors.forename: Murray
authors.initials: M
authors.lastname: Grant
authors.forename: Atle M
authors.initials: AM
authors.lastname: Bones
authors.forename: Richard
authors.initials: R
authors.lastname: Bennett
authors.forename: Yin Sze
authors.initials: YS
authors.lastname: Lim
authors.forename: Ralph
authors.initials: R
authors.lastname: Kissen
authors.forename: John T
authors.initials: JT
authors.lastname: Rossiter
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D004798:Enzymes / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D005296:Ferrous Compounds; D015870:Gene Expression; D005961:Glucosinolates / Q000378:metabolism*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D029681:Arabidopsis Proteins; D004798:Enzymes; D005296:Ferrous Compounds; D005961:Glucosinolates; D011994:Recombinant Proteins; C450151:epithiospecifier protein, Arabidopsis