Polymerase chain reaction of nanoparticle-bound primers.
Using one or two primers respectively bound to the surface of Au nanoparticles (AuNPs) or magnetic nanoparticles (MNPs), polymerase chain reaction (PCR) based on nanoparticles was systemically studied, agarose gel electrophoresis and atomic force microscopy (AFM) were respectively used to detect and observe the PCR product. The results obtained indicated that with either one or two primers respectively bound to the nanoparticle surface, PCR can proceed successfully under optimized condition and is subject to certain rules, consequently a symmetric PCR technique and an asymmetric PCR technique based on nanoparticles have been developed. A kind of nanostructured aggregates can be constructed by a symmetric PCR using two nanoparticle-bound primers.
Shen
He Bai
HB
Life and Environment Science College, Shanghai Normal University, PR China. Shenhb@shnu.edu.cn
Hu
Min
M
Wang
You Bao
YB
Zhou
Hai Qing
HQ
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D006046:Gold