title: Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH.
abstract: A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.
authors.affiliation: Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Ontario, Canada.
authors.forename: Tirukalikundram S
authors.initials: TS
authors.lastname: Kumaravel
authors.forename: Robert G
authors.initials: RG
authors.lastname: Bristow
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D018450:Disease Progression; D005260:Female; D016158:Genes, p53; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009153:Mutagens; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D009153:Mutagens; D018719:Erb-b2 Receptor Tyrosine Kinases