title: [Differentiation of bone marrow derived Thy-1+ beta2M- cells into liver cells in AA induced liver injury micro-environment].
abstract: OBJECTIVE
  To investigate the differentiation of bone marrow derived Thy-1+ beta2M- cells (BDTCs) into liver cells in allyl alcohol (AA) induced liver injury micro-environment.
  
  
  METHODS
  BDTCs of male F344 rats were isolated by two-step magnetic separation system (MACS) technique, and infused intraportally into female recipients after labeling with PKH26. Thirty recipients were divided randomly into 3 groups: (1) AA-injured liver + BDTCs infusion, (2) normal liver + BDTCs infusion and (3) AA-injured liver + NS infusion (control). Blood biochemical examination, fluorescence labeled cellular localization, Y-chromosome sry gene in-situ hybridization and immunohistochemistry were carried out to evaluate BDTCs distribution, differentiation and proliferation in recipients's livers after different intervals.
  
  
  RESULTS
  Fluoromicroscopy and in situ hybridization suggested that BDTCs of donors were interspersed in pieces and cords among the necro-periportals induced by AA; immunohistochemistry indicated that those implanted cells expressed OV-6, AFP, CK19 and albumin successively, while positive cells were hardly seen in the normal liver + BDTCs infusion group. Compared with the controls, the blood biochemical restitution was more rapid in group (1), (9.8 d +/- 3.1 d vs. 13.7 d +/- 4.2 d).
  
  
  CONCLUSIONS
  The injury micro-environment induced by AA facilitates BDTCs integration with hepatic cell plates and differentiation into mature liver cells. BDTCs differentiation into liver cells might accelerate endogenous liver cell regeneration and reparation.
authors.affiliation: Department of Stem Cell Biology, Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yun-Fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Nan
authors.forename: Cheng-Ze
authors.initials: CZ
authors.lastname: Yu
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Guan
authors.forename: Wen
authors.initials: W
authors.lastname: Yue
authors.forename: Yue-Tao
authors.initials: YT
authors.lastname: Pei
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology*; D022781:Hepatocytes / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D020005:Propanols; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D020005:Propanols; C006463:allyl alcohol