[Establishment of cytokine-independent human myelodysplastic cell line and its characteristics].
This study was aimed to establish a cytokine-independent human myelodysplastic cells line from bone marrow of a patient with MDS-CMML. This cell line was incubated in mixed culture of RPMI 1640 and DMEM with 15% bovine serum, but without cytokines; its biological characteristics were identified by morphology, surface marker profiles, cell proliferation, differentiation and apoptosis. The results showed that the established cell line could not depend on cytokines for long-term survival and growth, and could differentiate into colony-forming unit-macrophage, colony-forming unit-megakaryocyte. In conclusion, a cytokine-independent human myelodysplastic syndrome cell line, named MDS-JSN04 (MDS Nanjing Jiansu 04), was established. Its partial biological characteristics were identified and clarified.
Shao
Ze-Ye
ZY
Institute of MDS, Department of Hematology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, China.
Chen
Bao-An
BA
Xia
Guo-Hua
GH
Xue
Meng
M
Gao
Chong
C
Ding
Jia-Hua
JH
Sun
Yun-Yu
YY
Wang
Jun
J
Cheng
Jian
J
Zhao
Gang
G
Gao
Xue-Zhi
XZ
D018941:Antigens, CD19 / Q000032:analysis; D018951:Antigens, CD20 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051925:CD79 Antigens / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000378:metabolism / Q000473:pathology*; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000032:analysis; D013997:Time Factors
D018941:Antigens, CD19; D018951:Antigens, CD20; D051925:CD79 Antigens; D003470:Culture Media; D016207:Cytokines; D006684:HLA-DR Antigens; D016256:Lewis X Antigen; D051918:Sialic Acid Binding Ig-like Lectin 2