Telemetric monitoring of corpus spongiosum penis pressure in conscious rats for assessment of micturition and sexual function following spinal cord contusion injury.
Disruption of bladder function and sexual reflexes are major complications following spinal cord injury (SCI). We examined the use of telemetric monitoring of corpus spongiosum penis (CSP) pressures for assessment of micturition and erectile events following SCI in rats. Pressure catheters were implanted in the bulb of the CSP of seven male Long-Evans hooded rats, subjected to a standardized weight drop SCI (10 g x 12.5 mm) at T10. CSP pressures were analyzed for spontaneously occurring micturition and erectile events, and during ex copula reflex erection tests until 25 days after SCI. Urine volume was determined until 21 days after SCI. Results show initial loss of bladder function after SCI with gradual return of reflex micturition. When compared to baseline (BL), micturition pressure characteristics after SCI included prolonged duration, increased area under the curve (AUC), increased mean pressures, increased number of pressure peaks, and increased peak frequency. At 21 days after SCI, the urine volume per micturition was significantly increased. The number of full erectile events decreased significantly following SCI. Pressure wave analyses demonstrated increased AUC, increased maximum pressures, increased suprasystolic peak duration, increased AUC of the suprasystolic peaks, and increased maximum pressures of the suprasystolic peaks during recovery. The number of partial erectile events decreased significantly following SCI. Ex copula reflex erection testing demonstrated significantly decreased latency. The study demonstrates that telemetric monitoring of CSP pressures in conscious rats is a valuable and reliable method for assessing recovery of autonomic function following SCI.
Nout
Yvette S
YS
Department of Neuroscience, Laboratory of CNS Repair, and Spinal Trauma and Research (STAR) Laboratories, The Ohio State University, 333 W. 10th Avenue, Columbus, OH 43210, USA.
Schmidt
Markus H
MH
Tovar
C Amy
CA
Culp
Esther
E
Beattie
Michael S
MS
Bresnahan
Jacqueline C
JC
D000818:Animals; D003243:Consciousness; D003307:Copulation / Q000502:physiology; D004195:Disease Models, Animal; D006874:Hydrostatic Pressure; D008297:Male; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods*; D010413:Penis / Q000098:blood supply / Q000294:innervation / Q000503:physiopathology*; D051381:Rats; D020318:Rats, Long-Evans; D020127:Recovery of Function / Q000502:physiology; D012018:Reflex / Q000502:physiology; D012735:Sexual Dysfunction, Physiological / Q000209:etiology / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000150:complications / Q000503:physiopathology*; D013686:Telemetry / Q000295:instrumentation / Q000379:methods*; D014160:Transducers, Pressure; D014546:Urinary Catheterization; D014554:Urination / Q000502:physiology; D014555:Urination Disorders / Q000209:etiology / Q000503:physiopathology*