title: Polyclonality of familial murine adenomas: analyses of mouse chimeras with low tumor multiplicity suggest short-range interactions.
abstract: In previous studies demonstrating the polyclonal structure of familial intestinal adenomas, high tumor multiplicity made it difficult to eliminate the possibility that polyclonality arose by the random collision of distinct initiated clones as opposed to some form of clonal interaction. We sought to test further the random collision hypothesis. Chimeric mice carrying the multiple intestinal neoplasia (Min) mutation of the adenomatous polyposis coli gene (Apc) and homozygous for the tumor resistance allele of the Mom1 locus were established. These chimeras also display a strong propensity for tumors of polyclonal structure, despite their markedly reduced tumor multiplicity. Considering tumor sizes and multiplicities, the observed fraction of overtly polyclonal heterotypic adenomas was significantly higher than predicted by the random collision hypothesis. This finding supports models of polyclonality involving interaction among multiple initiated clones. The extent of clonal interaction was assessed by statistical analyses that relate the observed frequency of overtly polyclonal heterotypic tumors to the geometry of the chimeric patches and the pattern of underlying crypts. These statistical calculations indicate that the familial adenomas of the Apc(Min/+) mouse may commonly form through interactions between clones as close as 1-2 crypt diameters apart.
authors.affiliation: McArdle Laboratory for Cancer Research, Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Thliveris
authors.forename: Richard B
authors.initials: RB
authors.lastname: Halberg
authors.forename: Linda
authors.initials: L
authors.lastname: Clipson
authors.forename: William F
authors.initials: WF
authors.lastname: Dove
authors.forename: Ruth
authors.initials: R
authors.lastname: Sullivan
authors.forename: Mary Kay
authors.initials: MK
authors.lastname: Washington
authors.forename: Stephen
authors.initials: S
authors.lastname: Stanhope
authors.forename: Michael A
authors.initials: MA
authors.lastname: Newton
mesh_terms: D000236:Adenoma / Q000235:genetics*; D011125:Adenomatous Polyposis Coli / Q000235:genetics; D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D000483:Alleles; D000818:Animals; D002678:Chimera; D019295:Computational Biology / Q000379:methods; D003433:Crosses, Genetic; D018450:Disease Progression; D017491:Genes, APC; D006720:Homozygote; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D008958:Models, Molecular; D015233:Models, Statistical; D009154:Mutation; D010641:Phenotype; D012984:Software; D019076:Transgenes
chemical_list: D025601:Adenomatous Polyposis Coli Protein