A morphometric approach for the evaluation of angiogenesis in acute lymphoblastic leukemia of childhood.
Angiogenesis was estimated in childhood acute lymphoblastic leukemia (ALL) by the use of a novel morphometric method. This was compared with the widely used one of microvessel density (MVD). Bone marrow biopsies were evaluated by immunostaining with anti-Factor VIII related antigen (FVIIIRAg). Angiogenesis was calculated as volume corrected microvessel density index (VC-MVDI), taking into account the bone marrow cellularity. According to our results both MVD and VC-MVDI were increased at diagnosis of ALL in comparison with the control group. However the VC-MVDI increment was not statistically significant. Therefore, VC-MVDI could be more representative of the true increase of angiogenesis, correlating better with the outcome of the disease.
Kalmanti
Lida
L
Department of Medical Oncology, University Hospital of Heraklion, P.O. Box 1352, 71110 Heraklion Crete, Greece.
Dampaki
Konstantina
K
Dimitriou
Helen
H
Stiakaki
Eftichia
E
Chaniotis
Vretos
V
Stathopoulos
Efstathios
E
D000704:Analysis of Variance; D001854:Bone Marrow Cells / Q000473:pathology*; D001856:Bone Marrow Examination / Q000379:methods; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*