title: Expression of dihydropyrimidine dehydrogenase, thymidylate synthase, p53 and p21 in metastatic liver tumor from colorectal cancer after 5-fluorouracil-based chemotherapy.
abstract: BACKGROUND
  The expression of genes thought to be related to 5-FU chemosensitivity has been extensively investigated. However, little data is available on the expression patterns of these genes after chemotherapy.
  
  
  METHODS
  We investigated the expression of four genes, DPD, TS, p53 and p21, in the metastatic liver lesions obtained from colorectal cancer patients who had been treated with hepatic arterial infusions of 5-fluorouracil(5-FU)-based chemotherapy.
  
  
  RESULTS
  Expression of DPD, TS and p53 in the metastatic liver lesions was significantly higher in the chemotherapy-response group than in the no response group. In the response group, viable cancer cell nests were seen in confined spaces surrounded by fibrous tissue. It was of interest that these cancer cells in the response group showed conspicuous immunoreactivity of DPD, TS and p53.
  
  
  CONCLUSIONS
  An analysis of genes involved in 5-FU sensitivity revealed that surviving tumor cells exhibited resistance characteristics, indicating that the chemotherapy regimen should be altered, even in partially responding cases, unless the response is pathologically complete.
authors.affiliation: Yokohama City University Graduate School of Medicine, Department of Gastroenterological Surgery, Yokohama, Japan. s-gishi@kmc.mhc.east.ntt.co.jp
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamagishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimada
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Shouichi
authors.initials: S
authors.lastname: Fujii
authors.forename: Kuniya
authors.initials: K
authors.lastname: Tanaka
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Masui
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Togo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ike
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D005472:Fluorouracil; D006499:Hepatic Artery; D006801:Humans; D007150:Immunohistochemistry; D007261:Infusions, Intra-Arterial; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D015689:Oncogene Protein p21(ras); D005472:Fluorouracil