title: [Effect of phosphodiesterase inhibitor on expression of IL-8 mRNA in peripheral blood mononuclear cells from COPD patients].
abstract: OBJECTIVE
  To explore the inhibitory mechanism of phosphodiesterase(PDE) inhibitor on expression of IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) from chronic obstructive pulmonary disease(COPD) patients.
  
  
  METHODS
  PBMCs isolated from 20 COPD patients and 15 healthy subjects were co-cultured with non-selective inhibitor theophylline or PDE type IV inhibitor Rolipram. The expression of IL-8 mRNA was assayed by RT-PCR. The expression of NF-kappaB was determined by immunocytochemical staining. The content of I-kappaB protein was detected by Western blot.
  
  
  RESULTS
  The expression of IL-8 mRNA was elevated and the percentage of NF-kappaB nucleus positive cells was higher in COPD patients than in normal controls(P<0.01), while the expression of I-kappaB was lower in COPD patients (P<0.01). Theophylline of 1 mmol/L caused a decrease in the percentage of NF-kappaB nucleus positive cells (P<0.05). The expression of IL-8 mRNA and I-kappaB were not affected by theophylline at the dose of 100 micromol/L or 1 mmol/L. Rolipram inhibited the expression of IL-8 mRNA and the activation of NF-kappaB(P<0.05).
  
  
  CONCLUSIONS
  The results indicated that IL-8 mRNA may play a pathogenic role in the development of COPD and that selective PDE type IV inhibitor Rolipram inhibit the expression of IL-8 mRNA via NF-kappaB.
authors.affiliation: Department of Respiratory Diseases, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China. yangdanlei@126.com
authors.forename: Dan-lei
authors.initials: DL
authors.lastname: Yang
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Xu
authors.forename: Zhen-xiang
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Chao-qian
authors.initials: CQ
authors.lastname: Li
authors.forename: Wang
authors.initials: W
authors.lastname: Ni
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D020672:I-kappa B Proteins; D016209:Interleukin-8; D016328:NF-kappa B; D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger