title: Involvement of Bax, Bcl-2 and caspase 3 in hydroxyurea- or etoposide-induced apoptosis of mouse interleukin-3-dependent lymphoma cells.
abstract: BACKGROUND
  The apoptosis action induced by hydroxyurea or etoposide in interleukin 3-dependent lymphoma cells (DA-1) was studied.
  
  
  METHODS
  The conditions to study apoptosis of these cells were 17 hours of cell treatment with concentrations of 1.25 mM hydroxyurea or 100 microM etoposide using flow cytometry, fluorometry and immunoblots techniques.
  
  
  RESULTS
  Time-dependent reductions of cell viability after these treatments were observed. Caspase 3 activity was highly activated in both cases. Chemical treatments or interleukin 3 withdrawal rendered level changes in Bax and Bcl-2 expression.
  
  
  CONCLUSIONS
  These results support the implication of these factors in DA-1 cell apoptosis induced by these chemical treatments or interleukin 3 depletion.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Olmos
authors.forename: Alfredo
authors.initials: A
authors.lastname: Prieto
authors.forename: Angel
authors.initials: A
authors.lastname: Herráez
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Tejedor
authors.forename: Melchor
authors.initials: M
authors.lastname: Alvarez-Mon
authors.forename: José C
authors.initials: JC
authors.lastname: Diez
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D003470:Culture Media; D004790:Enzyme Induction / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein
chemical_list: D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; C494827:Bax protein, mouse; D003470:Culture Media; D007377:Interleukin-3; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D005047:Etoposide; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D006918:Hydroxyurea