Protective effects of ginseng components in a rodent model of neurodegeneration.
To test the proposed neuroprotective activity of whole ginseng extract and its components, we used 3-nitropropionic acid (3-NP), an inhibitor of succinate dehydrogenase, to produce neurodegeneration. Treatment with 3-nitropropionic acid (90 mg/kg) over a 5-day period resulted in severe impairment of movement and loss of neurons in the striatum. Pretreatment with a preparation from the whole root of American ginseng had no protective effects. Pretreatment with a preparation of ground leaves and stems, which contains greater levels of ginsenosides than ground root, improved the behavioral score and reduced the volume of the striatal lesion. A partial purification of American ginseng was performed to concentrate the putative protective components: Rb1, Rb3, and Rd (termed Rb extract). Pretreatment with the Rb extract significantly reduced the 3-nitropropionic acid-induced motor impairment and cell loss in the striatum, and it completely prevented any mortality. Significant effects on motor function, mortality, and the striatal lesion volume also were measured in animals pretreated with the individual ginsenosides, Rb1, Rb3, or Rd. The results demonstrate that some of the ginsenosides have neuroprotective activity, and that a partial purification of whole ginseng to concentrate the neuroprotective components may have utility as a neuroprotective agent.
Lian
Xiao-Yuan
XY
Department of Pharmacology, Baylor College of Medicine, Houston 77030, USA.
Zhang
Zhizhen
Z
Stringer
Janet L
JL
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D004791:Enzyme Inhibitors; D036145:Ginsenosides / Q000627:therapeutic use; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D017072:Neostriatum / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D009574:Nitro Compounds; D005894:Panax; D010936:Plant Extracts / Q000627:therapeutic use; D011422:Propionates; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D036145:Ginsenosides; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D010936:Plant Extracts; D011422:Propionates; D013385:Succinate Dehydrogenase; C015392:3-nitropropionic acid