Allogeneic dendritomas induce anti-tumour immunity against metastatic colon cancer.
Colon cancer (CC) is one of the most common malignancies in the Western world. Although surgical resection is the first choice worldwide, at this point an effective approach for the treatment of patients with metastasis and cancer recurrence post-operation has not yet been found. The aim of this study was to investigate the role of the allogeneic dendritomas from fusion of dendritic cells (DC) and metastatic CC cells in the activation of anti-tumour immunity against metastatic CC. Dendritomas were generated by fused allogeneic human peripheral blood DC with metastatic CC cells using 50% polyethylene glycol. The proliferation of the T cells and the toxicity of the cytotoxic T lymphocytes were observed after T cell pulsed with allogeneic dendritomas. The activated ratios of CD4+T helper 1 and CD8+Tc1 cells were about 51.55 and 65.60% after T cells were mixed with fusions for 24 h, which higher than those of controls. The proliferation of T cells were significantly higher than those of control after T cell pulsed with dendritomas (P < 0.01). Significantly, the activated CD8+ T lymphocytes effectively lysed the CC cells. These results demonstrate that allogeneic dendritomas activate T-cell responses against metastatic CC cells.
Xu
F
F
Division of Surgical Oncology and Division of Gastrointestinal Surgery, Peking University People's Hospital, Beijing, China P.R.
Ye
Y-J
YJ
Cui
Z-R
ZR
Wang
S
S
D002459:Cell Fusion; D003110:Colonic Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation; D009362:Neoplasm Metastasis; D011092:Polyethylene Glycols; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology
D011092:Polyethylene Glycols