[Effects of chromium supplementation on IRS-1 expression of skeletal muscle in diabetic rats].
OBJECTIVE
To study the effects of chromium supplementation on related gene expression to metabolism of skeletal muscles in diabetic rats.


METHODS
cDNA fragments from the out related research were cloned, sequenced and its comparalarity analysis has been done. The RT-PCR were made using the primers designed according to the sequence of cDNA.


RESULTS
The identities between the sequence of Cr-5 and IRS-1 is 100%. The expression level of DM group (0.791 +/- 0.038) is obviously lower than those of normal group (0.892 +/- 0.053, P < 0.05). And The expression level of DM + Cr group (0.822 +/- 0.066) have some improvement compare to those of DM group (P > 0.05).


CONCLUSIONS
There is a tendency that Chromium supplementation can increase the expression level of IRS-1 mRNA of skeletal muscles in diabetic rats. It will be studied as a candidate gene in future research.
Sun
Zhong
Z
College of Public Health, Tianjin Medical University, Tianjin 300070, China.
Wu
Yun-Tang
YT
Che
Su-Ping
SP
Wang
Xia
X
D000818:Animals; D001483:Base Sequence; D002857:Chromium / Q000008:administration & dosage / Q000494:pharmacology*; D018076:DNA, Complementary; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D012333:RNA, Messenger; D002857:Chromium