title: T-cell activation and memory phenotypes in cerebrospinal fluid during HIV infection.
abstract: We characterized T cell phenotypes in 74 paired blood and cerebrospinal fluid (CSF) samples of HIV-infected and uninfected persons using four-color flow cytometry. CD4+ and CD8+ T cells subsets were further characterized by identifying activated/resting and memory/naive subsets in CSF and blood using the markers CD38/HLA-DR and CD45RA/CD62L, respectively. With and without HIV-infection, the proportion of CD4+ T cells and memory T cells among T cells in CSF was higher compared to blood. In HIV-infection, activated CD4+ and CD8+ T cells in CSF were more abundant than in uninfected controls. As expected, combination antiretroviral therapy (ART) reduced T cell activation in CSF and blood.
authors.affiliation: Department of Neurology, San Francisco General Hospital, General Clinical Research Center at the University of California, San Francisco, CA 94158, USA. juttan@itsa.ucsf.edu
authors.forename: Jutta K
authors.initials: JK
authors.lastname: Neuenburg
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Cho
authors.forename: Annelie
authors.initials: A
authors.lastname: Nilsson
authors.forename: Barry M
authors.initials: BM
authors.lastname: Bredt
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Hebert
authors.forename: Robert M
authors.initials: RM
authors.lastname: Grant
authors.forename: Richard W
authors.initials: RW
authors.lastname: Price
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000134:cerebrospinal fluid* / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012367:RNA, Viral / Q000097:blood / Q000134:cerebrospinal fluid; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology*; D019562:Viral Load
chemical_list: D012367:RNA, Viral