title: Prostaglandin E1-initiated immune regulation during human mixed lymphocyte reaction.
abstract: Prostaglandin E1 (PGE1) has therapeutic value for transplantations due to its microvascular activity. Interleukin (IL)-18, which is elevated in plasma during the acute rejection after organ transplantation, elicits the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40, and CD40 ligand (CD40L) on monocytes as well as the production of interferon (IFN)-gamma and IL-12 and proliferation of T-cells during the human mixed lymphocyte reaction (MLR) in an in vitro model of acute rejection. In contrast, PGE1 inhibits all the adhesion molecule expression, cytokine production and T-cell proliferation in the presence of IL-18. The effects of PGE1 depend on stimulation of the IP/EP2/EP4-receptor, and thus, PGE1 might have therapeutic potential for treating acute rejection due to its immune regulatory effect.
authors.affiliation: Department of Pharmacology, Okayama University Graduate School of Medicine and Dentistry, Okayama City 700-8558, Japan.
authors.forename: Hideo K
authors.initials: HK
authors.lastname: Takahashi
authors.forename: Dong
authors.initials: D
authors.lastname: Xue
authors.forename: Hiromi
authors.initials: H
authors.lastname: Iwagaki
authors.forename: Ryuji
authors.initials: R
authors.lastname: Tamura
authors.forename: Goutarou
authors.initials: G
authors.lastname: Katsuno
authors.forename: Takahito
authors.initials: T
authors.lastname: Yagi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
authors.forename: Shuji
authors.initials: S
authors.lastname: Mori
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishibori
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000096:biosynthesis / Q000276:immunology; D023201:CD40 Ligand / Q000096:biosynthesis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D020382:Interleukin-18 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; D020382:Interleukin-18; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1; D023201:CD40 Ligand; D007371:Interferon-gamma; D000527:Alprostadil