title: Cyclic stretch induced MAPK phosphorylation stronger than static stretch.
abstract: The environment for living organism in space has microgravity and/or hypergravity and/or any kind of mechanical stresses. Cellular response may differ from the variety of mechanical stress. Mitogen-activated protein kinases (MAPKs) pathway is related to various cellular events. In the present study it was investigated the serial measurement of MAPK phosphorylation using western-blotting analysis following with three types of cyclic stretch, static, 0.1 Hz and 0.25 Hz. The result was that induction of MAPK phosphorylation had peaks within 2 to 4 hours and attenuated, while induction of p38 phosphorylation in 0.1 Hz stretch had a peak at 6 hours later and the strongest. Thus, there might be differential cellular response depends upon the frequency of cyclic stretch .
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Chiba Univ.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kanazawa
authors.forename: Jun
authors.initials: J
authors.lastname: Nomura
authors.forename: Juni
authors.initials: J
authors.lastname: Himeta
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshimoto
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ichinose
mesh_terms: D015153:Blotting, Western; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D010766:Phosphorylation; D015398:Signal Transduction / Q000502:physiology*; D013314:Stress, Mechanical; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases