RAPD & FINS (mitochondrial 16s rRNA gene sequence) in analysis of food contamination: detection of food poisoning by Lizard.
In poisoning, detection of the nature of causative agent is important for management of trauma and forensic investigation. Most of the methods in clinical toxicology are developed for detection of toxins and poisons. A random amplified polymorphic DNA (RAPD)-based method has been described for detection of species of animal from its morphologically unrecognizable fragments, recovered from food substances, consumption of which caused even death. Pre-mixed RAPD reaction beads and six RAPD primers were used in polymerase chain reaction analysis. Among six RAPD primers used, any one of them was sufficient in resolving this practical forensic situation. To enhance the probability values for matching in the present study of fixing identity of an animal, six set of market available RAPD primers were used. This is the first report of a forensic application of RAPD DNA typing in identification of charred skeleton remnants of Lizard species in food material. Furthermore unique amplicons were generated for different reptilian species, which can be used as species specific markers for species identification in forensic situation, however, no variations among individuals of same species were observed.
Chattopadhyay
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Forensic Lab, Bollarm Road, Hyderabad, India; Amrutha Valley, Banjara Hills, Hyderabad 34, India. cprabal@yahoo.com
Guha
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D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry; D004785:Environmental Pollutants / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005517:Foodborne Diseases / Q000175:diagnosis* / Q000209:etiology; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D007194:India; D008116:Lizards / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
D004272:DNA, Mitochondrial; D004785:Environmental Pollutants; C034477:RNA primers; D012336:RNA, Ribosomal, 16S; D012313:RNA