Effect of testosterone and steroids homologues on indolamines and lipid peroxidation in rat brain.
The purpose of the present study was to evaluate the effect of 4-pregnen-17-hydroxy-3-one (A) and two steroids homologues: 3beta-acetoxy-5,16-pregnadien-20-one (B) and 3beta-acetoxy-16alpha-17alpha-epoxy-4-pregnen-20-one (C). Male Wistar rats were treated with o-cresol combined (A, B or C) steroids. Lipid peroxidation status as result of measurement reactive substances to thiobarbituric acid (TBARS) as well as serotonin (5-HT) and its precursor 5-hydroxytryptophan (5-HTP) were measured. The prostate glands were weighed, the 5alpha-reductase activity was determined. The animals treated with A, B, and C steroids showed a slight increase in both 5alpha-reductase activity and prostate size. 5-HT and 5-HTP levels did not change significantly, and TBARS showed an increase in the group treated with B steroid and a decrease in the A steroid group with significant differences in both groups (p<0.05) versus control group. Results suggest that A steroid reduces TBARS in rat brain, perhaps as a result of the interaction between the testosterone unsaturated carbons and OH(-) groups with free radicals.
Guzmán
David Calderón
DC
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Avenida Imán No. 1, 3er piso, Colonia Cuicuilco, CP 04530 México City, Mexico.
Mejía
Gerardo Barragán
GB
Vázquez
Ivonne Espitia
IE
García
Ernestina Hernández
EH
del Angel
Daniel Santamaría
DS
Olguín
Hugo Juárez
HJ
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D042944:Cholestenone 5 alpha-Reductase / Q000378:metabolism; D003408:Cresols / Q000494:pharmacology; D006908:Hydroxyprogesterones / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D011282:Pregnenediones / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
C469666:3-acetoxypregna-5,16-diene-20-one; D003408:Cresols; D006908:Hydroxyprogesterones; D011282:Pregnenediones; D017392:Thiobarbituric Acid Reactive Substances; C067746:17-hydroxypregn-4-ene-3-one; D012701:Serotonin; D013739:Testosterone; D006916:5-Hydroxytryptophan; D042944:Cholestenone 5 alpha-Reductase; C034047:2-cresol