New methods for the generation of carbohydrate arrays on glass slides and their evaluation.
Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino-functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precluding the necessity for the blocking of unreacted functional groups on the glass slide. The covalency and the concentration dependency of the sugar ligation to the glass slide were demonstrated; the reversibility and the selectivity of lectin-carbohydrate interactions were shown.
Biskup
Moritz B
MB
Fachbereich Chemie, Universität Konstanz, Fach M 725, 78457 Konstanz, Germany.
Müller
Jan U
JU
Weingart
Ralf
R
Schmidt
Richard R
RR
D000577:Amides / Q000737:chemistry; D000586:Amination; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006027:Glycosides / Q000138:chemical synthesis; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008832:Microchemistry / Q000379:methods*
D000577:Amides; D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D006027:Glycosides; D037102:Lectins