title: Chronic periodontal disease is associated with single-nucleotide polymorphisms of the human TLR-4 gene.
abstract: Periodontitis is an inflammatory disease affecting the connective tissue surrounding the teeth leading to tooth loss. Pathogens associated with periodontitis interact with Toll-like receptors (TLRs) to induce cytokines causing and aggravating disease. We screened 197 individuals suffering from generalized periodontitis for the presence of Asp299Gly and Thr399Ile of TLR-4 as well as Arg753Gln of TLR-2 in comparison to matched controls. Single-nucleotide polymorphisms (SNPs) of TLR-4 were elevated among patients (odd's ratio 3.650, 95% CI 1.573-8.467, P < or = 0.0001), while no difference was observed for TLR-2. TLR-4 SNPs were correlated with chronic periodontitis (odd's ratio 5.562, 95% CI 2.199-14.04, P < or = 0.0001), but not with aggressive periodontitis. This observation was confirmed employing a group of periodontally healthy probands over 60 years of age. These data demonstrate that genetic variants of TLR-4 may act as risk factors for the development of generalized chronic periodontitis in humans.
authors.affiliation: Institute for Microbiology and Hygiene, Charité University Medical Center Berlin, Berlin, Germany.
authors.forename: N W J
authors.initials: NW
authors.lastname: Schröder
authors.forename: D
authors.initials: D
authors.lastname: Meister
authors.forename: V
authors.initials: V
authors.lastname: Wolff
authors.forename: C
authors.initials: C
authors.lastname: Christan
authors.forename: D
authors.initials: D
authors.lastname: Kaner
authors.forename: V
authors.initials: V
authors.lastname: Haban
authors.forename: P
authors.initials: P
authors.lastname: Purucker
authors.forename: C
authors.initials: C
authors.lastname: Hermann
authors.forename: A
authors.initials: A
authors.lastname: Moter
authors.forename: U B
authors.initials: UB
authors.lastname: Göbel
authors.forename: R R
authors.initials: RR
authors.lastname: Schumann
mesh_terms: D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4