Towards normalization of soybean somatic embryo maturation.
Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal salt formulations were tested in an effort to modify existing soybean embryogenesis histodifferentiation/maturation media to further normalize the development of soybean somatic embryos. The resultant liquid medium, referred to as soybean histodifferentiation and maturation medium (SHaM), consists of FNL basal salts, 3% sucrose, 3% sorbitol, filter-sterilized 30 mM glutamine and 1 mM methionine. SHaM-derived somatic embryos are more similar to seed in terms of protein and fatty acid/lipid composition, and conversion ability, than somatic embryos obtained from traditional soybean histodifferentiation and maturation media.
Schmidt
M A
MA
Center for Applied Genetic Technologies, 111 Riverbend Road, University of Georgia, Athens, GA 30602-6810, USA. mschmidt@danforthcenter.org
Tucker
D M
DM
Cahoon
E B
EB
Parrott
W A
WA
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005973:Glutamine / Q000378:metabolism / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D012492:Salts / Q000378:metabolism / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism / Q000494:pharmacology; D013025:Glycine max / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D010940:Plant Proteins; D012492:Salts; D005973:Glutamine; D013012:Sorbitol; D013395:Sucrose; D000040:Abscisic Acid; D008715:Methionine