[Evaluation of different liquid media for the culture of Gardnerella vaginalis and characterization of the protein profile with PAGE-SDS electrophoresis].
3 liquid culture media: triptose soya broth supplemented with equine serum (TSB-es)/without supplement (TSB) and Casman broth (CB) were evaluated under different incubation conditions at 10% of CO2 and under aerobic conditions. There were no significant differences in the values of optic density for the TSB-es and CB media. However, on establishing the protein profile by PAGE-SDS for the evaluated conditions, the presence of protein of 14 kDa and the values of optic density allowed the selection of the CB medium for the culture of Gardnerella vaginalis. The sensitivity to different pH in this medium was studied. pH 7.2 proved to be optimum and it was established its growth cynetics. The analysis by PAGE-SDS of the complete cells corresponding to different clinical isolates of G. vaginalis with biochemical variations made possible to characterize the protein profile in this medium and showed similarity between the isolates.
Espinosa
Ivette
I
Centro Nacionial de Sanidad Agropecuaria, Autopista Nacional San José de las Lajas. espinosa@censa.edu.cu
Lorenzo
Maité
M
Riverón
Yamilka
Y
Alvarez
Elba
E
Villoch
Alejandra
A
D001426:Bacterial Proteins / Q000032:analysis; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006194:Gardnerella vaginalis / Q000254:growth & development* / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media