Quantification of SSX mRNA expression in human bone and soft tissue tumors using nucleic acid sequence-based amplification.
The SSX family proteins have been considered new members of the cancer/testis antigens because of the restricted expression in testis among normal tissues and the activation in a wide range of cancers. Thus, they would be potential molecular targets for immunotherapeutic strategies. We have developed a competitive nucleic acid sequence-based amplification (NASBA) assay to analyze SSX mRNA expression in 211 bone and soft tissue tumors. The copy numbers of SSX mRNA per mug of total RNA in tumor tissues were widely distributed, ranging logarithmically from 0.6 to 6.6. We found that malignant tumors showed significantly higher expression of SSX mRNA than benign tumors (P < 0.0001). Further, SSX mRNA expression in stage III tumors was significantly higher than that in stage I or II tumors (P < 0.005). This NASBA assay was also more sensitive compared to immunohistochemistry using newly affinity-purified polyclonal antibody against SSX. Collectively, these results suggest that the SSX quantitative NASBA assay could provide useful information to select eligible patients for SSX-specific cancer vaccines.
Naka
Norifumi
N
Department of Orthopedic Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-2 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
Joyama
Susumu
S
Tsukamoto
Yoshitane
Y
Yoshioka
Kiyoko
K
Hashimoto
Nobuyuki
N
Ujiiye
Takeshi
T
Hayashi
Tsukasa
T
Kawase
Masako
M
Mano
Masayuki
M
Ishiguro
Shingo
S
Myoui
Akira
A
Ueda
Takafumi
T
Yoshikawa
Hideki
H
Araki
Nobuhito
N
Itoh
Kazuyuki
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000276:immunology; D019556:COS Cells; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics*; D021161:Self-Sustained Sequence Replication; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000276:immunology
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012097:Repressor Proteins; C093962:synovial sarcoma X breakpoint proteins