[Expression of MMP-2 and MMP-9 in the tartrate-resistant acid phosphatase (TRAP)-positive mononuclear and multinucleated cells is correlated with articular cartilage damage].
OBJECTIVE
To examine the role of matrix metalloproteinases(MMP) expressed by the tartrate-resistant acid phosphatase (TRAP)-positive mononuclear and multinucleated cells in articular cartilage damage.


METHODS
C57BL/6 mice were immunized by injection of chicken type II(CII) collagen to construct the collagen-induced rheumatoid arthritis(CIA) model. The presence of TRAP positive cells in the synovial tissue of CIA mice was examined by enzyme histochemistry and expression of MMP-2,9 was assessed in TRAP positive cells by immunohistochemistry.


RESULTS
Expression of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) was detected in TRAP positive mononuclear and multinucleated cell. Quantity of TRAP positive cells and the destruction of articular cartilage had a positive correlation (r(s) =0.903, P<0.01). Expression of MMP-2 and MMP-9 in TRAP positive cells was also correlated significantly with the destruction of articular cartilage (r(s) =0.954, P<0.01).


CONCLUSIONS
This study suggests that MMP-2 and MMP-9 expression by TRAP positive mononuclear and multinucleated cells are involved in articular cartilage destruction in CIA.
Zhao
Wen
W
Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Zhu
Ping
P
Wang
Yan-hong
YH
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D002358:Cartilage, Articular / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D002467:Cell Nucleus / Q000378:metabolism*; D003094:Collagen / Q000009:adverse effects; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D003094:Collagen; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9