Apoptosis and necrosis in developing cerebellum and brainstem induced after focal cerebral hypoxic-ischemic injury.
Focal cerebral hypoxia-ischemia due to isolated vascular insufficiency is well known to cause ipsilateral, but not contralateral, cerebral apoptosis. Hypoxic-ischemic damage to the cerebellum and brainstem in such a model has not been established. This experimental rodent study demonstrates, through deoxyribonucleic acid fragmentation and terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'-triphosphate-digoxigenin nick end labeling analysis, that neuronal cells in these infratentorial regions also suffer mild apoptosis and necrosis after focal cerebral hypoxic-ischemic injury in the newborn rat. These data provide additional insight into the mechanisms of neurological injury in the cerebellum and brainstem areas resulting from a focal cerebral hypoxic-ischemic insult and demonstrate that future therapeutic interventions for hypoxic-ischemic encephalopathy system should deal with the entire central nervous system.
Peng
Jeng-Hsiung F
JH
jhfpeng@yahoo.com
Feng
Yangzheng
Y
LeBlanc
Michael H
MH
Rhodes
Philip G
PG
Parker
Joseph C
JC
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D015139:Blotting, Southern / Q000379:methods; D001933:Brain Stem / Q000254:growth & development / Q000473:pathology* / Q000503:physiopathology; D002531:Cerebellum / Q000254:growth & development / Q000473:pathology* / Q000503:physiopathology; D053938:DNA Fragmentation / Q000502:physiology; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology* / Q000503:physiopathology; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D009336:Necrosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley