Structure of the rotor ring of F-Type Na+-ATPase from Ilyobacter tartaricus.
In the crystal structure of the membrane-embedded rotor ring of the sodium ion-translocating adenosine 5'-triphosphate (ATP) synthase of Ilyobacter tartaricus at 2.4 angstrom resolution, 11 c subunits are assembled into an hourglass-shaped cylinder with 11-fold symmetry. Sodium ions are bound in a locked conformation close to the outer surface of the cylinder near the middle of the membrane. The structure supports an ion-translocation mechanism in the intact ATP synthase in which the binding site converts from the locked conformation into one that opens toward subunit a as the rotor ring moves through the subunit a/c interface.
Meier
Thomas
T
Institut für Mikrobiologie, Eidgenössische Technische Hochschule (ETH), Zürich Hönggerberg, Wolfgang-Pauli-Str. 10, CH-8093 Zürich, Switzerland.
Polzer
Patrick
P
Diederichs
Kay
K
Welte
Wolfram
W
Dimroth
Peter
P
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003593:Cytoplasm / Q000378:metabolism; D045182:Fusobacteria / Q000201:enzymology*; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D017136:Ion Transport; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D001426:Bacterial Proteins; D020409:Molecular Motor Proteins; D021122:Protein Subunits; D018698:Glutamic Acid; D012964:Sodium; D000251:Adenosine Triphosphatases; C500333:F-type sodium ATPase, Ilyobacter tartaricus