title: Cloning and expression of a novel isoform of heterogeneous nuclear ribonucleoprotein-R.
abstract: In the present study, we cloned a novel isoform of heterogeneous nuclear ribonucleoprotein-R (hnRNP-R), hnRNP-R2. Compared with the previously reported hnRNP-R (hnRNP-R1), it lacks the exon 5. Two proteins are both nuclear. The expression of hnRNP-R1 is not tissue-specific and is strikingly higher than that of hnRNR-R2. In comparison, the expression of hnRNP-R2 is low and neural-specific. The expression of two isoforms is differentially regulated during brain development. The results suggest that hnRNP-R may contribute to the regulation of neural development through the variation of hnRNP-R components in the hnRNP complex.
authors.affiliation: Laboratory of Genomic Physiology and Center for Brain Research, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Huang
authors.forename: Xian-hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology* / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D012322:RNA Precursors / Q000235:genetics
chemical_list: C439930:HNRNPR protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D020033:Protein Isoforms; D012322:RNA Precursors; D049452:Green Fluorescent Proteins