Molecular recognition of cyclic-nucleotides and current sensors for their detection.
This work briefly describes available sensors for cAMP and cGMP. Many sensors are based on derivatization of naturally occurring products such as immunoglobulins, protein kinases, etc. Only a few published works deal with chemosensors, which are built up by "total" chemical synthesis. This field stays opened for combinatorial chemist. The best sensors are protein kinases genetically modified with mutants of green fluorescent protein, which allow screening of entire cell cultures.
Sebestík
Jaroslav
J
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of CR, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Hlavácek
Jan
J
Stibor
Ivan
I
D015374:Biosensing Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009712:Nucleotides, Cyclic / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D015290:Second Messenger Systems; D015398:Signal Transduction / Q000502:physiology
D009712:Nucleotides, Cyclic; D049452:Green Fluorescent Proteins; D011494:Protein Kinases