Phenotypic and genotypic characteristics of new euploid-diploid lymphoblastoid B cell lines EBV+, normal human bone marrow derived, spontaneously overgrown in vitro.
The present study describes the phenotypic and genotypic features of seven individual growth transformed, euploid-diploid EBV+ human B cell lines arisen spontaneously in vitro. The lines, obtained under general and standard culture conditions (un-manipulated), from seven individual bone marrow samples of 18 healthy young adults, Caucasian, of both sexes, display many traits of normal B cells and represent a mixture of EBV infected latently (latency type III) and producer cells (5-16% VCA+ by immunofluorescence) releasing seven individual different viral strains [Fruscalzo et al., 2001. DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication. Gene 265, 165-173] similar to the B95-8 genotype as shown by results of Southern blot of BamHI-digested DNA fragment. These tests were planned to characterize more fully this panel of new bone marrow cell lines sharing normal B cell traits.
Bertolini
Luisa
L
Istituto di Neorobiologia e Medicina Molecolare, CNR, Via del Fosso del Cavaliere, 100, 00133 Rome, Italy. fruscalzo@uniroma1.it
Aebischer
Maria Luisa
ML
Ameglio
Franco
F
Angeloni
Antonio
A
Delaroche
Isabella
I
Faggioni
Alberto
A
Fruscalzo
Alberto
A
Gorini
Giovanni
G
Serafino
Annalucia
A
Starace
Giuseppe
G
Tabilio
Antonio
A
D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000821:virology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002453:Cell Cycle; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002472:Cell Transformation, Viral; D005260:Female; D015870:Gene Expression; D015322:Gene Rearrangement, B-Lymphocyte; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D017735:Virus Latency
D012333:RNA, Messenger; D012367:RNA, Viral