Identification of genes differentially expressed in Atlantic salmon (Salmo salar) in response to infection by Aeromonas salmonicida using cDNA microarray technology.
The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of each of three immune-relevant tissues (spleen, head kidney and liver) were obtained from fish exposed to infected salmon for 13 days. Reverse transcription-PCR assays were used to verify the differential expression of 12 candidate genes uncovered by microarray analysis. Among the differentially expressed genes were several previously revealed by suppression subtractive hybridization and EST surveys and that are recognized to encode humoral components of the innate immune system. Other genes identified in this study were not previously associated with infection. In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity.
Ewart
K Vanya
KV
Institute for Marine Biosciences, Halifax, NS, Canada B3H 3Z1.
Belanger
Jasmine C
JC
Williams
Jason
J
Karakach
Tobias
T
Penny
Susanne
S
Tsoi
Stephen C M
SC
Richards
Robert C
RC
Douglas
Susan E
SE
D048409:Aeromonas salmonicida; D000818:Animals; D018076:DNA, Complementary; D005667:Furunculosis / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D020317:Salmo salar / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*
D018076:DNA, Complementary