Tissue distribution, synthesis stage, and ethylene induction of pineapple (Ananas comosus) chitinases.
We examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. Type A (acidic class III) exists in all tissues, while type B (weakly basic class I, which has strong antifungal activity) and type C (acidic class I) are localized mainly in the leaf and stem. In a pericarp, type A exists at all stages during fruit development, while type B and type C exist only at the early stage. Synthesis of type A is induced by ethylene, while that of types B and C is not affected by it. These results suggest that the physiological roles of these three types of chitinase in pineapple are different.
Taira
Toki
T
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University, Okinawa 903-0213, Japan. tokey@agr.u-ryukyu.ac.jp
Toma
Noriko
N
Ichi
Marika
M
Takeuchi
Makoto
M
Ishihara
Masanobu
M
D031230:Ananas / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D002688:Chitinases / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D005638:Fruit / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant
D005030:Ethylenes; C036216:ethylene; D002688:Chitinases