title: Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing.
abstract: One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Aparna
authors.initials: A
authors.lastname: Girish
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.forename: Dawn S Y
authors.initials: DS
authors.lastname: Yeo
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Teck-Khiang
authors.initials: TK
authors.lastname: Chua
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D047668:Inteins; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins