title: Old yellow enzyme interferes with Bax-induced NADPH loss and lipid peroxidation in yeast.
abstract: The yeast transcriptional response to murine Bax expression was compared with the changes induced by H(2)O(2) treatment via microarray technology. Although most of the Bax-responsive genes were also triggered by H(2)O(2) treatment, OYE3, ICY2, MLS1 and BTN2 were validated to have a Bax-specific transcriptional response not shared with the oxidative stress trigger. In knockout experiments, only deletion of OYE3, coding for yeast Old yellow enzyme, attenuated the rate of Bax-induced growth arrest, cell death and NADPH decrease. Lipid peroxidation was completely absent in DeltaOYE3 expressing Bax. However, the absence of OYE3 sensitized yeast cells to H(2)O(2)-induced cell death, and increased the rate of NADPH decrease and lipid peroxidation. Our results clearly indicate that OYE3 interferes with Bax- and H(2)O(2)-induced lipid peroxidation and cell death in Saccharomyces cerevisiae.
authors.affiliation: Unit of Fundamental and Applied Molecular Biology, Department for Molecular Biomedical Research, VIB-Ghent University, Technologiepark 927, B-9052 Zwijnaarde, Belgium.
authors.forename: Rieka
authors.initials: R
authors.lastname: Reekmans
authors.forename: Kris
authors.initials: K
authors.lastname: De Smet
authors.forename: Cuiying
authors.initials: C
authors.lastname: Chen
authors.forename: Paul
authors.initials: P
authors.lastname: Van Hummelen
authors.forename: Roland
authors.initials: R
authors.lastname: Contreras
mesh_terms: D017209:Apoptosis; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation; D009249:NADP / Q000032:analysis / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C494827:Bax protein, mouse; D051028:bcl-2-Associated X Protein; D009249:NADP; D006861:Hydrogen Peroxide; D009252:NADPH Dehydrogenase