Nek8 mutation causes overexpression of galectin-1, sorcin, and vimentin and accumulation of the major urinary protein in renal cysts of jck mice.
The jck murine model, which results from a double point mutation in the nek8 gene, has been used to study the mechanism of autosomal recessive polycystic kidney disease (ARPKD). The renal proteome of jck mice was characterized by two-dimensional gel electrophoresis combined with mass spectrometry (MALDI-TOF/TOF). Four newly identified proteins were found to accumulate in the kidneys of jck mice with polycystic kidney disease (PKD) compared with their wild-type littermates. The proteins galectin-1, sorcin, and vimentin were found to be induced 9-, 9-, and 25-fold, respectively, in the PKD proteome relative to the wild type. The identity of these proteins was established by peptide mass fingerprinting and de novo MS/MS sequencing of selected peptides. Up-regulation of these three proteins may be due to the nek8 mutation, and their function may be related to the signaling and structural processes in the primary cilium. Additionally a series of protein isoforms observed only in the ARPKD kidney was identified as the major urinary protein (MUP). Peptide sequencing demonstrated that the isoforms MUP1, MUP2, and MUP6 are contained in this series. The MUP series showed a number of male-specific isoforms and a phosphorylation of the entire series with an increasing degree of phosphorylation of the acidic isoforms. In addition, the MUP series was localized to the cyst fluid of PKD mice, and a cellular mislocalization of galectin-1, sorcin, and vimentin in PKD tubular epithelial cells was shown. The abnormal and extremely high accumulation of the MUPs in the ARPKD kidney may be linked to a defect in protein transport and secretion. The discovery of these proteins will provide new information on the molecular and cellular processes associated with the mechanism of ARPKD.
Valkova
Nelly
N
Physiological Genomics Group, Department of Animal Science, University of California, Davis, California 95616, USA.
Yunis
Reem
R
Mak
Sally K
SK
Kang
Kiandra
K
Kültz
Dietmar
D
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D037483:Galectin 1 / Q000096:biosynthesis*; D007679:Kidney Medulla / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D000072379:NIMA-Related Kinases; D017044:Polycystic Kidney, Autosomal Recessive / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D014746:Vimentin / Q000096:biosynthesis*
D002135:Calcium-Binding Proteins; D037483:Galectin 1; D020033:Protein Isoforms; D011506:Proteins; D020543:Proteome; C503114:Sri protein, mouse; D014746:Vimentin; C035737:major urinary proteins; D011494:Protein Kinases; D000072379:NIMA-Related Kinases; C499219:Nek8 protein, mouse; D017346:Protein Serine-Threonine Kinases