title: Evaluation of chemosensitivity testing with highly purified tumor cells in 435 patients with gastric carcinoma using an MTT assay.
abstract: BACKGROUND
  We evaluated the results of chemosensitivity testing for gastric cancer using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in terms of the correlation of chemosensitivity and clinicopathological findings.
  
  
  METHODS
  We analyzed 435 consecutive patients with gastric cancer treated between January 1991 and January 2002. Highly purified fresh human gastric cancer cells were obtained from 485 lesions including 415 primary tumors and 70 metastatic tumors.
  
  
  RESULTS
  CDDP and 5-FU were more potent drugs than MMC, ADR and VP-16. The chemosensitivity of metastatic tumors was lower than that in primary tumors. The chemosensitivity in differentiated cancer was equivalent to that in undifferentiated cancer. The manner of tumor invasion and clinical stage affected chemosensitivity for some drugs.
  
  
  CONCLUSIONS
  Our results suggest that individual chemosensitivity testing is essential to individualize chemotherapy for gastric cancer.
authors.affiliation: Second Department of Surgery, Wakayama Medical University, School of Medicine, Wakayama, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Noguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Iwahashi
authors.forename: Masaji
authors.initials: M
authors.lastname: Tani
authors.forename: Masaki
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mikihito
authors.initials: M
authors.lastname: Nakamori
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ueda
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ishida
authors.forename: Teiji
authors.initials: T
authors.lastname: Naka
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Ojima
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Hotta
authors.forename: Shizuma
authors.initials: S
authors.lastname: Mizobata
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamaue
mesh_terms: D002945:Cisplatin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005047:Etoposide / Q000494:pharmacology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; D016685:Mitomycin; D005047:Etoposide; D004317:Doxorubicin; C022616:thiazolyl blue; D002945:Cisplatin; D005472:Fluorouracil