title: Second harmonic imaging of exocytosis at fertilization.
abstract: Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time.
authors.affiliation: Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030-1507, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Millard
authors.forename: Mark
authors.initials: M
authors.lastname: Terasaki
authors.forename: Leslie M
authors.initials: LM
authors.lastname: Loew
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D005089:Exocytosis / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005456:Fluorescent Dyes; D047331:Lytechinus / Q000502:physiology; D008297:Male; D011726:Pyridinium Compounds
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds