[The role of CXCL16 in immunological liver injury induced by BCG and LPS in mice].
OBJECTIVE
To investigate the pathophysiological role of CXCL16 in immunological liver injury induced by Bacille de Calmette et Guerin (BCG) and lipopolysaccharides (LPS).


METHODS
Immunological liver injury was induced by BCG and LPS in mice, and the expression of CXCL16 was detected in the liver tissues by real-time quantitative PCR and immunohistochemical examination. The relationship of the expression of CXCL16 and the extent of hepatic necrosis was investigated histopathologically and immunohistochemically. Mononuclear cells were isolated from the liver tissues and their numbers were counted; T lymphocytes populations in the liver tissue were also analyzed with FACS.


RESULTS
The immunological liver injury model was successfully created. Up-regulation of CXCL16 in injured livers correlated with the extent of liver injury and the amountmononuclear cell infiltrations.


CONCLUSIONS
These findings suggest that up-regulation of CXCL16 was closely correlated with liver injury extent during the immunological liver injury induced by BCG-LPS in mice, and intrahepatic recruitment of specific lymphocytes might be an important mechanism of liver injury.
Xu
Huan-Bin
HB
Department of Immunology, Shanghai Medical College, Fudan University, Key Laboratory of Molecular Medicine of the Ministry of Education, Shanghai 200032, China.
Gong
Yan-Ping
YP
Jiang
Zheng-Gang
ZG
Liu
Rui-Zi
RZ
Xiong
Si-Dong
SD
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis; D051116:Receptors, Scavenger / Q000096:biosynthesis* / Q000235:genetics
D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC; C498926:Cxcl16 protein, mouse; D008070:Lipopolysaccharides; D051116:Receptors, Scavenger