title: Assignment of the gene for porcine insulin-like growth factor binding protein 1 to chromosome 18 and detection of polymorphisms in intron 2 by PCR-RFLP.
abstract: We have obtained a partial cDNA and three BAC clones for the porcine insulin-like growth factor binding protein 1 gene (IGFBP-1). Results of fluorescence in situ and radiation hybrid (RH) mapping assigned this gene to porcine chromosome (SSC) 18q24-qter. We found two types of polymerase chain reaction-restriction-fragment-length polymorphisms (PCR-RFLP) in intron 2 by using FokI and AluI.
authors.affiliation: Society for Techno-innovation of Agriculture, Forestry and Fisheries Institute, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Ibaraki, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Inage-Miyake
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Shimanuki
authors.forename: Tomohito
authors.initials: T
authors.lastname: Itoh
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Murakami
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Masashi
authors.initials: M
authors.lastname: Miyake
authors.forename: Daisuke
authors.initials: D
authors.lastname: Toki
authors.forename: Hirohide
authors.initials: H
authors.lastname: Uenishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Awata
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hamasima
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D017404:In Situ Hybridization, Fluorescence; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics*; D007438:Introns / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*
chemical_list: D018970:Insulin-Like Growth Factor Binding Protein 1