Comparison of serological tests for the detection of ovine and caprine antibody to Brucella melitensis.
The indirect enzyme-linked immunosorbent assay (IELISA), the competitive enzyme-linked immunosorbent assay (CELISA) and the fluorescence polarisation assay (FPA) were evaluated with sera from sheep experimentally infected with Brucella melitensis and negative Canadian sheep. The sensitivity and specificity of the assays were as follows: IELISA: 91.7% and 97.6%, CELISA: 75.0% and 99.8% and FPA: 91.7% and 89.5%. Sera from the same experimental population were divided according to serological reaction in the rose bengal agglutination test (RBT) and the complement fixation test (CFT). Reactivity relative to the RBT positive and CFT positive sera were as follows: IELISA: 99.7%, CELISA: 93.2% and FPA: 99.1%. Since sera from goats with proven B. melitensis infection were not available, 699 sera from goats judged positive in the buffered antigen plate agglutination test (BPAT) and CFT and 982 BPAT/CFT negative Canadian goats were used. The sensitivity and specificity of the assays relative to the BPAT and CFT positive sera were: IELISA: 99.4% and 98.0%, CELISA: 95.4% and 97.1% and FPA: 92.7% and 99.8%.
Nielsen
K
K
Canadian Food Inspection Agency, Animal Diseases Research Institute, Ottawa, K2H 8P9 Ontario, Canada.
Gall
D
D
Smith
P
P
Balsevicius
S
S
Garrido
F
F
Ferrer
M Durán
MD
Biancifiori
F
F
Dajer
A
A
Luna
E
E
Samartino
L
L
Bermudez
R
R
Moreno
F
F
Renteria
T
T
Corral
A
A
D000372:Agglutination Tests / Q000379:methods / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D016231:Fluorescence Polarization Immunoassay / Q000379:methods / Q000662:veterinary; D015511:Goat Diseases / Q000175:diagnosis* / Q000276:immunology; D006041:Goats; D011897:Random Allocation; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial