title: Human umbilical cord blood cells express neurotrophic factors.
abstract: Freshly isolated or culture-expanded human umbilical cord blood mononuclear cells (CBMNCs) have been known to express neural phenotypes in vitro and to differentiate into neural cells and improve neurological function recovery after being administrated into rodent models of neurological diseases. However, the mechanism of action remains unclear. The present study observed that CBMNCs expressed higher level mRNAs of several neurotrophic factors than adult peripheral blood mononuclear cells (PBMCs). In addition, a significantly increase in the levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT4/5) was found in culture supernatants of CBMNCs compared to that of PBMNCs. These findings indicate that CBMNCs express several neurotrophic factors and suggest that the neurotrophic factors secreted by CBMNCs may be responsible for amelioration of central nervous system deficits in animal models after CBMNC administration.
authors.affiliation: Department of Neurosurgery, Peking University People's Hospital, Beijing, People's Republic of China.
authors.forename: Cun-Gang
authors.initials: CG
authors.lastname: Fan
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Zhang
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Tang
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Han
authors.forename: Ge-Sheng
authors.initials: GS
authors.lastname: Wang
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020127:Recovery of Function / Q000502:physiology; D012038:Regeneration / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C073922:neurotrophin 4