title: [Induction and culture of hairy-root by Agrobacteriun rhizogenes in Dendrobium nobile].
abstract: OBJECTIVE
  To obtain the hairy-roots of Dendrobium nobile.
  
  
  METHODS
  The hairy-root were obtained by infecting different explants with Agrobacteriun rhizogenes A4 and culture conditions of hariy root were optimized.
  
  
  RESULTS
  The best conditions of hairy root culture were 1/2MS medium with sucrose (3%) as carbon material and lactalbumin hydrolyze (1g/L) as nitron material.
authors.affiliation: Biology Department, Zunyi Normal College, Zunyi 563002.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Li
authors.forename: Shaohu
authors.initials: S
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Sun
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis; D003470:Culture Media; D031667:Dendrobium / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D012231:Rhizobium; D027343:Rhizome / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D000470:Alkaloids; D003470:Culture Media