Peroxiredoxin I and II are up-regulated during differentiation of epidermal keratinocytes.
Peroxiredoxins (Prxs) are expressed in the epidermis, and the accentuated expression of the Prx I and Prx II isotypes (Prx I/II) in the suprabasal layers suggests the potential role of Prx I/II in epidermal differentiation. To evaluate the novel function of Prx I/II, we checked the modulation of Prx I/II in differentiating keratinocytes. To induce differentiation in vitro, normal human epidermal keratinocytes (NHEK) were cultured for up to 10 days after the confluent state (post-confluency). In Western blot analysis, the marked induction of Prx I was observed from the second day, but the marked induction of Prx II was observed later from the sixth day of post-confluency, when loricrin and transglutaminase 1 were induced (sixth day of post-confluency). When NHEK cells were treated with INF-gamma and TGF-beta1, Prx I/II were up-regulated by INF-gamma, but Prx I/II were down-regulated by TGF-beta1. In summary, Prx I and Prx II are induced at the early and late stage of differentiation of NHEK cells, respectively.
Yun
Sook Jung
SJ
Department of Dermatology, Chonnam National University Medical School, 8 Hak-dong, Gwangju, 501-757, Korea (South).
Seo
Jae Jung
JJ
Chae
Jee Young
JY
Lee
Seung-Chul
SC
D002454:Cell Differentiation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D006360:Heat-Shock Proteins / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D010544:Peroxidases / Q000502:physiology*; D054464:Peroxiredoxins; D017382:Reactive Oxygen Species; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation
D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D007371:Interferon-gamma; D010544:Peroxidases; C086065:PRDX2 protein, human; D054464:Peroxiredoxins