Fluorometric method for quantitative determination of glucose and its application to human serum.
By coupling the Fenton reaction with the glucose oxidase (GOD)-catalyzed reaction, we have developed a fluorometric method for the determination of glucose; the linear response range and the detection limit are 0.108-2.59 microg/ml and 0.0432 microg/ml, respectively. This approach is selective and has the advantages of using inexpensive reagents and avoiding interferences. Satisfactory results were obtained for the determination of glucose in human serum samples.
Gao
Jigang
J
College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018 Shandong, People's Republic of China.
Zhou
Jie
J
Qu
Xiangjin
X
D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D005290:Ferric Compounds / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D016877:Oxidants; D011804:Quinolines / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D005290:Ferric Compounds; D007202:Indicators and Reagents; D016877:Oxidants; D011804:Quinolines; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase