title: Effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose} on human hepatocellular carcinoma cell line.
abstract: OBJECTIVE
  To study the effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2).
  
  
  METHODS
  HepG2 cells were cultured in RPMI-1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.
  
  
  RESULTS
  At the concentration ranging between 1-30 micromol/L, COPADG potently inhibited the growth and induced apoptosis of HepG2 cells.
  
  
  CONCLUSIONS
  COPADG could effectively induce apoptosis in human hepatocellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. wujing36@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Kou
authors.forename: Ming-tai
authors.initials: MT
authors.lastname: Gao
authors.forename: Yong-ning
authors.initials: YN
authors.lastname: Zhou
authors.forename: Ai-qin
authors.initials: AQ
authors.lastname: Wang
authors.forename: Qun-ji
authors.initials: QJ
authors.lastname: Xue
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D046529:Microscopy, Electron, Transmission
chemical_list: C495189:2-(3-carboxy-1-oxopropyl)amino-2-deoxy-D-glucose; D000970:Antineoplastic Agents; D003847:Deoxyglucose