Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry.
The six phenolic constituents are water-soluble components extracted from the Chinese medical herb danshen, the dried roots of Salvia miltiorrhiza Bunge (Labiatae). An liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based method has been developed for the simultaneous quantification of six phenolic constituents of danshen (magnesium lithospermate B (MLB), rosmarinic acid (RA) and lithospermic acid (LA), caffeic acid (CAA), protocatechuic aldehyde (3,4-dihydroxybenzaldehyde, Pal), 3,4-dihydroxyphenyllactic acid (danshensu)) in human serum with chloramphenicol as internal standard. The serum samples were treated by special liquid-liquid extraction, and the analytes were determined using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode, with sufficient sensitivity to allow analysis of human serum samples generated following administration of a clinically relevant dose. Good linearity over the range 8-2048 ng/mL for six phenolic constituents was observed. The intra- and inter-day precisions (CV) of analysis were <13%, and the accuracy ranged from 88 to 116%. This quantitation method was successfully applied to a pharmacokinetic study of i.v. drip infusion of Danshen injection fluid in human.
Li
Xiaochuan
X
Department of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China; Graduate School of the Chinese Academy of Sciences, China. xchli@sibs.ac.cn
Yu
Chen
C
Cai
Yongbao
Y
Liu
Gangyi
G
Jia
Jingying
J
Wang
Yiping
Y
D001547:Benzaldehydes / Q000097:blood; D001572:Benzofurans / Q000097:blood; D002109:Caffeic Acids / Q000097:blood; D002396:Catechols / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D002934:Cinnamates / Q000097:blood; D053630:Depsides; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis; D006801:Humans; D007773:Lactates / Q000097:blood; D010636:Phenols / Q000097:blood*; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000097123:Rosmarinic Acid
D001547:Benzaldehydes; D001572:Benzofurans; D002109:Caffeic Acids; D002396:Catechols; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D007773:Lactates; D010636:Phenols; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C061091:lithospermate B; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; C040048:caffeic acid