title: [The effect of pcDNA 3.1/RPs15 on skin fibroblasts in vitro].
abstract: OBJECTIVE
  To construct eukaryotic expression vector of ribosomal protein sl5(RPs15) gene and study its effect on mouse skin fibroblasts in vitro.
  
  
  METHODS
  The RPs15 cDNA encoding region of fetal mouse skin was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method and cloned into eukaryotic expression vector pcDNA3.1(-). The recombinant plasmid was transfected into adult mouse skin fibroblasts by FuGENE6 transfection reagents. Then the expression of RPs15 gene, was detected and its biological effect on fibroblasts was measured.
  
  
  RESULTS
  The DNA sequencing result of pcDNA3.1/RPs15 was identical with the reported. The RPs15 gene was expressed in transfected fibroblasts. The growth density of fibroblasts decreased with the conformation changing accordingly.
  
  
  CONCLUSIONS
  The eukaryotic expression vector pcDNA3.1/RPs15 is successively constructed and can be expressed in mouse skin fibroblasts. The results set up a basis for further study of the effect of RPs15 gene on skin fibroblasts.
authors.affiliation: Institute of Plastic Surgery, Weifang Medical University, Weifang 261042, China.
authors.forename: Sheng-jian
authors.initials: SJ
authors.lastname: Tang
authors.forename: Xiao-ke
authors.initials: XK
authors.lastname: Wang
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: LV
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005822:Genetic Vectors; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D012269:Ribosomal Proteins; D012867:Skin / Q000166:cytology; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D009687:Nuclear Proteins; D012269:Ribosomal Proteins; C069798:Rps15 protein, mouse