title: Differences in invasive capacity of endometrial cancer cell lines expressing different progesterone receptor isotypes: possible involvement of cadherins.
abstract: OBJECTIVE
  Loss of expression of progesterone receptors (PR) in endometrial cancer is related to a more invasive and metastatic phenotype. In this study we aim to investigate whether selective loss of PRA or PRB affects the invasive capacity of endometrial cancer cells.
  
  
  METHODS
  cDNA microarrays were performed to compare gene expression profiles of a set of endometrial cancer sub-cell lines expressing PRA and/or PRB. In vitro invasion assays were performed to assess whether differences in gene expression between the lines were reflected by their invasive behavior.
  
  
  RESULTS
  It was observed that cell lines that express only PRA express higher levels of cadherins, and show a lower level of invasion compared to cell lines that express PRB. When cadherin function was inhibited in exclusively PRA-expressing cell lines, an increase of in vitro invasion was observed. In support of these findings, it was observed that in higher grade and more invasive endometrial cancer, expression of E-cadherin decreased.
  
  
  CONCLUSIONS
  These results indicate that relative loss of PRA during progression of endometrial cancer can have a negative impact on cadherin expression, which may lead to development of a more metastatic phenotype.
authors.affiliation: Department of Reproduction and Development, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: Eline E
authors.initials: EE
authors.lastname: Hanekamp
authors.forename: Susanne C J P
authors.initials: SC
authors.lastname: Gielen
authors.forename: Petra E
authors.initials: PE
authors.lastname: De Ruiter
authors.forename: Savi
authors.initials: S
authors.lastname: Chadha-Ajwani
authors.forename: Frans J
authors.initials: FJ
authors.lastname: Huikeshoven
authors.forename: Curt W
authors.initials: CW
authors.lastname: Burger
authors.forename: J Anton
authors.initials: JA
authors.lastname: Grootegoed
authors.forename: Leen J
authors.initials: LJ
authors.lastname: Blok
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000096:biosynthesis; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000503:physiopathology*; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011980:Receptors, Progesterone / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D015820:Cadherins; D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B