Yeast Ypt11 is targeted to recycling endosomes in mammalian cells.
BACKGROUND
In yeast, Ypt11 or Ypt32 along with the highly homologous Ypt8 or Ypt31 has been reported to be an essential component of intra-Golgi trafficking and has been implicated in the budding of vesicles from the most distal Golgi compartment.


CONCLUSIONS
In the present study, we show that, in human cells, after heterologous expression of GFP-Ypt11 (where GFP stands for green fluorescent protein), the protein is targeted to transferrin-positive recycling endosomes. This compartment has been shown to form extensive tubular networks on applying the drug Brefeldin A. We also show, by confocal fluorescent microscopy, that these networks also contain Rab11 in cells expressing CFP-Rab11a (where CFP stands for cyan fluorescent protein) fusion protein and that these structures are identical with those targeted by GFP-Ypt11.
Kail
Mark
M
Department of Experimental Tumorbiology, University Muenster, Badestr. 9, 48149 Muenster, Germany. mkali@uni-muenster.de
Hollinshead
Mike
M
Kaufmann
Miriam
M
Boettcher
Jens
J
Vaux
David
D
Barnekow
Angelika
A
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D011992:Endosomes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012235:Rhodamines / Q000032:analysis; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014162:Transfection; D014168:Transferrin / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000099193:rab11 GTP-Binding Proteins
D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D029701:Saccharomyces cerevisiae Proteins; D014168:Transferrin; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C468556:Ypt11 protein, S cerevisiae; C054562:yellow fluorescent protein, Bacteria