Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas.
The DNA sequence of the gyrB gene is a suitable phylogenetic marker for bacterial systematics. In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach. PCR primers targeting the gyrB gene of aeromonads were designed and the resulting amplicons were analyzed by DGGE. The gyrB-DGGE analysis was evaluated with Aeromonas isolates and reference strains allowing discrimination of the majority of strains. The gyrB-DGGE analysis is a powerful tool to monitor the presence and evaluate the diversity of aeromonads in complex samples, as is the case of water from a wastewater treatment plant.
Tacão
Marta
M
Center for Cell Biology, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Moura
Alexandra
A
Alves
Artur
A
Henriques
Isabel
I
Saavedra
Maria José
MJ
Correia
António
A
D000333:Aeromonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D020459:Genes, rRNA; D009691:Nucleic Acid Denaturation; D017686:Oncorhynchus mykiss / Q000382:microbiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014871:Water Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase