Molecular cloning and in vitro evaluation of an infectious simian-human immunodeficiency virus containing env of a primary Chinese HIV-1 subtype C isolate.
Human immunodeficiency virus (HIV) clade C is the most prevalent subtype and accounts for approximately 50% of all HIV infections worldwide. In China, the prevalent HIV strains are B'/C subtypes, in which the envelope belongs to subtype C. To evaluate potential AIDS vaccines targeting Chinese viral strains in non-human primate models, we constructed an infectious simian-human immunodeficiency virus (SHIV) that expresses most of the envelope of a primary HIV strain, which was isolated from a HIV-positive intravenous drug user from XinJiang province in China. The resulting chimeric SHIV-XJ02170 was infectious in human, rhesus monkey and cynomolgus monkey peripheral blood mononuclear cells (PBMC) and used CCR5 exclusively as coreceptor.
Wu
YingYun
Y
College of Life Sciences, NanKai University, TianJin, China.
Hong
KunXue
K
Chenine
Agnès-Laurence
AL
Whitney
James B
JB
Xu
Weidong
W
Chen
QiMin
Q
Geng
YunQi
Y
Ruprecht
Ruth M
RM
Shao
Yiming
Y
D000818:Animals; D002681:China; D003001:Cloning, Molecular; D005818:Genetic Engineering; D019302:HIV Envelope Protein gp160 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D019713:Receptors, CCR5 / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014779:Virus Replication
D019302:HIV Envelope Protein gp160; D012367:RNA, Viral; D019713:Receptors, CCR5