Cytochrome P450 biosensors-a review.
Cytochrome P450 (CYP) is a large family of enzymes containing heme as the active site. Since their discovery and the elucidation of their structure, they have attracted the interest of scientist for many years, particularly due to their catalytic abilities. Since the late 1970s attempts have concentrated on the construction and development of electrochemical sensors. Although sensors based on mediated electron transfer have also been constructed, the direct electron transfer approach has attracted most of the interest. This has enabled the investigation of the electrochemical properties of the various isoforms of CYP. Furthermore, CYP utilized to construct biosensors for the determination of substrates important in environmental monitoring, pharmaceutical industry and clinical practice.
Bistolas
Nikitas
N
Department of Analytical Biochemistry, University of Potsdam, Karl-Liebknecht-Street 24-25, 14476 Golm, Germany.
Wollenberger
Ulla
U
Jung
Christiane
C
Scheller
Frieder W
FW
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D020099:Coated Materials, Biocompatible; D003577:Cytochrome P-450 Enzyme System