[Effect of acid suppression therapy for eradicating Helicobacter pylori infection on bismuth absorption from colloidal bismuth pectin].
OBJECTIVE
To investigate whether acid suppression therapy influences the absorption of bismuth from colloidal bismuth pectin (CBP).


METHODS
48 male SD rats were randomly divided into five groups to be administer with different medicines once a day for 14 days: group A1 (administered with CBP only and killed on the cessation day of administration), group B1 (administered with CBP only and killed 8 weeks after the cessation of administration), group A2 [administered with CBP + amoxicillin (AMO) + metronidazole (MTR) + losec and killed on the cessation day of administration], group B2 (administered with CBP + AMO + MTR + losec and killed 8 weeks after the cessation of administration), and control group (administered with distilled water). The kidney issue sections were counterstained after AMG development. The bismuth deposited in tissues was observed by microscopy. The gray level of kidney tissue sections were measured and compared through image processing program. The deposition of bismuth and the degrees of cell organ's impairment were observed by electron microscopy. By using electron probe microanalysis bismuth was identified from the chemical elements in the specimens.


RESULTS
Under the light microscopy, black-brown granules were discovered in the cell bodies of the proximal convoluted renal tubule. The amounts of bismuth accumulated in kidney of the 2 quadruple therapy groups were much more than those of the 2 single compound therapy groups (all P < 0.05). The amount of bismuth accumulated in kidney on the cessation day of administration was more than that eight weeks later (both P < 0.01). Under electron microscopy, black-brown granules were observed exclusively in the lysosomes of the proximal convoluted renal tubule cell. Electron microscopy found cell impairment in the quadruple therapy groups. Impairment of these cells could be recovered 8 weeks after the cessation of administration.


CONCLUSIONS
Acid suppression therapy causes an increase of absorption and accumulation of bismuth from CBP in the kidney. Bismuth can be accumulated in the cell bodies of proximal convoluted renal tubule after its absorption. The absorbed bismuth can be discharged out of the body via kidney. Large amounts of bismuth accumulation in kidney can impair the functions of proximal convoluted renal tubule cells.
Zhang
Li
L
Department of Gastroenterology, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Zhang
Shu-tian
ST
Yu
Zhong-lin
ZL
Shao
Ying
Y
Wang
Yuan-shen
YS
Yang
Hui
H
D000658:Amoxicillin / Q000493:pharmacokinetics; D000818:Animals; D000863:Antacids / Q000009:adverse effects*; D001729:Bismuth / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D003102:Colloids / Q000008:administration & dosage / Q000493:pharmacokinetics; D004359:Drug Therapy, Combination; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008795:Metronidazole / Q000493:pharmacokinetics; D010368:Pectins / Q000008:administration & dosage / Q000493:pharmacokinetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000863:Antacids; D003102:Colloids; D008795:Metronidazole; D000658:Amoxicillin; D010368:Pectins; D001729:Bismuth