[Scanning electron microscopic observations of Monochamus alternatus antennal sensilla and their electroantennographic responses].
With scanning electron microscope (SEM), this paper observed the shape, category, amount and distribution of the main antenna sensilla of adult Monochamus alternatus, and tested their electroantennographic (EAG) responses to the main volatiles of Pinus spp.. There were seven types of antennal sensilla, i. e., sensilla trichoid, sensilla basiconica, sensilla digit-like, sensilla rod-like, sensilla bottle-like, sensilla bud-like and sensilla chaetica, among which, sensilla trichoid and sensilla basiconica were the most abundant on the antenna surface, and each of them could be divided into three subtypes. Two subtypes of sensilla digit-like could also be observed. The II and III subtypes of sensilla trichoid and I and II subtypes of sensilla basiconica had deep longitudinal grooves on their surface, the typical characteristics of olfactory receptor. The comparison of the EAG response of different parts of Monochamus alternatus antennae to alpha-Pinene showed that each volatile and their compounds could provoke significant EAG responses of both females and males. The dose-response test showed that there was a certain rule in the EAG responses of M. alternatus.
Wang
Sibao
S
Institute of Plant Physiology & Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. wangsibao@hotmail.com
Zhou
Hongchun
H
Miao
Xuexia
X
Fan
Meizhen
M
Li
Zengzhi
Z
Si
Shengli
S
Huang
Yongping
Y
D000818:Animals; D001517:Coleoptera / Q000502:physiology / Q000648:ultrastructure*; D028223:Pinus / Q000254:growth & development / Q000469:parasitology*; D010935:Plant Diseases / Q000469:parasitology*; D011984:Sensory Receptor Cells / Q000502:physiology / Q000648:ultrastructure*