title: The capsaicin-sensitive afferent neuron in skeletal muscle is abnormal in heart failure.
abstract: BACKGROUND
  In heart failure, the cardiovascular response to activation of the skeletal muscle exercise pressor reflex (EPR) is exaggerated. Group IV afferent neurons, primarily stimulated by the metabolic by-products of skeletal muscle work, contribute significantly to the EPR. Therefore, it was postulated that alterations in the activity of group IV neurons contribute to the EPR dysfunction manifest in heart failure.
  
  
  RESULTS
  Group IV afferent fibers were ablated in neonatal Sprague-Dawley rats by subcutaneous administration of capsaicin. In neonatal capsaicin-treated adult animals, selective activation of the EPR, by electrically induced static muscle contraction, recapitulated the exaggerated increases in heart rate and blood pressure observed in rats with dilated cardiomyopathy (DCM). Furthermore, compared with control animals, both neonatal capsaicin-treated and DCM rats displayed a decreased pressor response to the intra-arterial administration of capsaicin within the hindlimb, a maneuver that selectively excites group IV afferent neurons. Moreover, expression of mRNA for the capsaicin receptor TRPv1, a marker of group IV fibers, was downregulated in DCM animals compared with controls.
  
  
  CONCLUSIONS
  These findings suggest that EPR dysfunction in heart failure results in part from functional and molecular alterations in group IV fibers. Furthermore, the responsiveness of these metabolically sensitive neurons appears to be blunted in DCM, indicating that their contribution to the EPR may be reduced. This occurs despite an overall exaggeration of the EPR in heart failure. These insights into the basic mechanisms of EPR dysfunction are essential to the development of effective therapeutic strategies aimed at improving exercise capacity in heart failure.
authors.affiliation: Department of Internal Medicine, Harry S. Moss Heart Center, University of Texas Southwestern Medical Center, Dallas, TX 75390-9174, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Smith
authors.forename: Maurice A
authors.initials: MA
authors.lastname: Williams
authors.forename: Jere H
authors.initials: JH
authors.lastname: Mitchell
authors.forename: Pradeep P A
authors.initials: PP
authors.lastname: Mammen
authors.forename: Mary G
authors.initials: MG
authors.lastname: Garry
mesh_terms: D000818:Animals; D017704:Baroreflex; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology*; D002311:Cardiomyopathy, Dilated / Q000503:physiopathology; D015536:Down-Regulation; D006333:Heart Failure / Q000503:physiopathology*; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000294:innervation*; D009475:Neurons, Afferent / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000235:genetics
chemical_list: D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D002211:Capsaicin