title: Isothermal RNA sequence amplification method for rapid antituberculosis drug susceptibility testing of Mycobacterium tuberculosis.
abstract: RNA transcript quantification by an isothermal sequence amplification reaction was evaluated for susceptibility testing of 15 Mycobacterium tuberculosis strains. Agreement with the proportion method on Ogawa egg medium and the BACTEC MGIT 960 system was 100 and 87% for rifampin, 93 and 100% for isoniazid, 60 and 53% for ethambutol, and 80 and 80% for streptomycin, respectively.
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Kyoto, 606-8507, Japan. stakakr@kuhp.kyoto-u.ac.jp
authors.forename: Shunji
authors.initials: S
authors.lastname: Takakura
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujihara
authors.forename: Toyoichiro
authors.initials: T
authors.lastname: Kudo
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Iinuma
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mitarai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ichiyama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasukawa
authors.forename: Takahiko
authors.initials: T
authors.lastname: Ishiguro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012329:RNA, Bacterial / Q000187:drug effects*; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D012329:RNA, Bacterial