title: Integrin messenger RNAs in the red nucleus after axotomy and neurotrophic administration.
abstract: Integrins are cell surface receptors known to be important for regeneration in the peripheral nervous system. We have investigated the expression of integrin messenger RNAs in red nucleus neurons of adult rats after axotomy and administration of neurotrophic factors. Using radioactive in situ hybridization, messenger RNA for integrin subunits beta1, alpha3, alpha7 and alphaV could be detected. No change of any alpha subunit could be detected after axotomy. In contrast, a small upregulation of beta1 was detected after lesion. Administration of neurotrophin-3 induced a robust further increase in beta1 messenger RNA levels, whereas brain-derived neurotrophic factor did not. By analogy to the peripheral nervous system, we propose that integrins may be important for a regenerative response in central nervous system neurons.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, S-17177 Stockholm, Sweden. stefan.plantman@neuro.ki.se
authors.forename: Stefan
authors.initials: S
authors.lastname: Plantman
authors.forename: Liudmila
authors.initials: L
authors.lastname: Novikova
authors.forename: Lev
authors.initials: L
authors.lastname: Novikov
authors.forename: Henrik
authors.initials: H
authors.lastname: Hammarberg
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Wallquist
authors.forename: Jan-Olof
authors.initials: JO
authors.lastname: Kellerth
authors.forename: Staffan
authors.initials: S
authors.lastname: Cullheim
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001345:Autoradiography; D019771:Axotomy; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D005260:Female; D017403:In Situ Hybridization; D039001:Integrin alpha Chains / Q000235:genetics; D039422:Integrin alpha3 / Q000235:genetics; D039564:Integrin alphaV / Q000235:genetics; D019012:Integrin beta1 / Q000235:genetics*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D020933:Neurotrophin 3 / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012012:Red Nucleus / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000503:physiopathology*; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D019208:Brain-Derived Neurotrophic Factor; D039001:Integrin alpha Chains; D039422:Integrin alpha3; D039564:Integrin alphaV; D019012:Integrin beta1; D020933:Neurotrophin 3; D012333:RNA, Messenger; C085725:integrin alpha7