Mass spectrometry-based proteomics in the life sciences.
Over the last 20 years, mass spectrometry-based proteomics has become an indispensable tool in the cellular and molecular life sciences. This has been enabled by the 'soft ionisation' techniques of electrospray and matrix-assisted laser desorption-ionisation, which allow the gentle ionisation and vaporisation of large, thermally labile biomolecules. Innovative instrumentation designs and biochemical strategies have brought success in the large-scale identification and quantification of proteins, as well as the characterisation of their complexes and post-translational modifications. This review describes the instrumentation used for proteomics research. It presents an overview of the current applications of mass spectrometry-based proteomics to the cellular and molecular life sciences, and discusses challenges that exist for research in the future.
Lane
C S
CS
Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom. catherine.lane@ulsop.ac.uk
D000818:Animals; D019295:Computational Biology; D006801:Humans; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
D007477:Ions; D010455:Peptides; D011506:Proteins