Differential effects of clozapine on ethanol-induced ascorbic acid release in mouse and rat striatum.
Previous studies have shown that acute systemic administration of ethanol-induced striatal ascorbic acid (AA) release in mice and rats. In the present study, in vivo brain microdialysis coupled with high performance liquid chromatography (HPLC) with electrochemical detection (ECD) was used to comparatively evaluate the effects of clozapine on ethanol-induced AA release in mouse and rat striatum. The results showed that clozapine, at the dose of 15 mg/kg i.p., had no effect on basal AA or ethanol-induced AA release in rat striatum. The potentiating effect of clozapine on ethanol-induced striatal AA release was still observed in rats, at the higher dose of 30 mg/kg. In contrast, clozapine significantly inhibited ethanol-induced AA release in mouse striatum, at the dose of 15 and 30 mg/kg, without affecting basal AA release. The present study suggested that clozapine differentially regulated ethanol-induced AA release in the mouse and rat striatum.
Hou
Yue
Y
Department of Pharmacology, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
Wu
Chun Fu
CF
Yang
Jing Yu
JY
Tu
Ling
L
Gu
Pei Fei
PF
Bi
Xiu Li
XL
D000704:Analysis of Variance; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001921:Brain; D001923:Brain Chemistry / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003024:Clozapine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D018756:GABA Antagonists / Q000494:pharmacology*; D008297:Male; D051379:Mice; D017551:Microdialysis / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D013997:Time Factors
D002492:Central Nervous System Depressants; D018756:GABA Antagonists; D000431:Ethanol; D003024:Clozapine; D001205:Ascorbic Acid