Study on the chromosomal karyotype and G-banding of Alpacas (Lama pacos).
Blood samples from 23 Huacaya alpacas, 3 males and 20 females, were used to study chromosomes and karyotypes, so as to provide some effective cytogenetic bases for the selection, improvement by crossing, disease diagnosis of alpacas, and genetic mechanisms of sex determination. Peripheral blood lymphocyte culture was used to prepare chromosome. A method of trypase-EDTA was used for G-banding. The results showed as follows: The number of diploid chromosomes was 2n=74, with the karyotype 74, XY and 74, XX for males and females respectively. Thirty-six homologous pairs of chromosomes were autosomes, in which chromosomes pairs No.1 to No.20 were acrocentric-subterminal and No.21 to No.36 metacentric-submetacentric. And X chromosome was metacentric, Y chromosome telocentric. The analysis of G-bands showed that bright and dark bands appeared by turn. It showed different bands. And every pair of chromosomes had its distinct band, and the longer the chromosomes, the more the number of bands, and the more clear the bands.
Zhang
Qiao-Ling
QL
College of Animal Science and Technology, Shanxi Agricultural University, TaiGu, China. zql2323@sohu.com
Dong
Chang-Sheng
CS
He
Jun-Ping
JP
He
Xiao-Yan
XY
Fan
Rui-Wen
RW
Geng
Jian-Jun
JJ
Ren
Yu-Hong
YH
D000818:Animals; D002161:Camelids, New World / Q000235:genetics*; D002871:Chromosome Banding; D033481:Chromosomes, Mammalian / Q000235:genetics*; D004171:Diploidy; D005260:Female; D007621:Karyotyping; D008297:Male; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics