Molecular typing of West Nile Virus, Dengue, and St. Louis encephalitis using multiplex sequencing.
We report the development of an assay to simultaneously identify three of the clinically important flaviviruses (West Nile Virus, Dengue, and St. Louis encephalitis). This assay is based on the nucleotide sequence variations within a 266-bp region of the non-structural protein 5. Further, based on the nucleotide variations in the same region of the non-structural protein 5, four of the present Dengue serotypes were identified. To identify some of the subtypes of WNV we have developed a second assay using multiplex sequencing technology. The format of the result of this assay is an electropherogram of two genomic segments of the WNV genome: a 48-nucleotide sequence from the anchored core protein C and a 45-nucleotide sequence coding for the non-structural proteins (proteinase and putative helicase genes).
Vinayagamoorthy
Thuraiayah
T
Bio-ID Diagnostic Inc., Saskatoon, Saskatchewan, Canada. moorthy@bio-id-diagnostic.com
Mulatz
Kirk
K
Drebot
Michael
M
Hodkinson
Roger
R
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004665:Encephalitis Virus, St. Louis / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D014902:West Nile virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral