title: Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.
abstract: The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher degree of catalytic promiscuity and lower total turnover in comparison with their natural counterparts. Using bacterial display and multiparameter flow cytometry, we have developed a novel methodology for emulating positive and negative selective pressure in vitro for the isolation of enzyme variants with reactivity for desired novel substrates, while simultaneously excluding those with reactivity toward undesired substrates. Screening of a large library of random mutants of the Escherichia coli endopeptidase OmpT led to the isolation of an enzyme variant, 1.3.19, that cleaved an Ala-Arg peptide bond instead of the Arg-Arg bond preferred by the WT enzyme. Variant 1.3.19 exhibited greater than three million-fold selectivity (-Ala-Arg-/-Arg-Arg-) and a catalytic efficiency for Ala-Arg cleavage that is the same as that displayed by the parent for the preferred substrate, Arg-Arg. A single amino acid Ser223Arg substitution was shown to recapitulate completely the unique catalytic properties of the 1.3.19 variant. These results can be explained by proposing that this mutation acts to "swap" the P(1) Arg side chain normally found in WT substrate peptides with the 223Arg side chain in the S(1) subsite of OmpT.
authors.affiliation: Institute for Cellular and Molecular Biology and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA.
authors.forename: Navin
authors.initials: N
authors.lastname: Varadarajan
authors.forename: Jongsik
authors.initials: J
authors.lastname: Gam
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Olsen
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
authors.forename: Brent L
authors.initials: BL
authors.lastname: Iverson
mesh_terms: D001120:Arginine / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D002469:Cell Separation; D018360:Crystallography, X-Ray; D004798:Enzymes / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D005434:Flow Cytometry; D015723:Gene Library; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008958:Models, Molecular; D009154:Mutation; D010447:Peptide Hydrolases; D010455:Peptides / Q000737:chemistry; D018272:Porins / Q000235:genetics*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D055672:Static Electricity; D013379:Substrate Specificity
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004798:Enzymes; D029968:Escherichia coli Proteins; D010455:Peptides; D018272:Porins; C499588:ompT protein, E coli; D001120:Arginine; D010447:Peptide Hydrolases