Identification of serum proteins related to adverse effects induced by docetaxel infusion from protein expression profiles of serum using SELDI ProteinChip system.
BACKGROUND
For the development of quick and easy methods for screening and identifying treatment-responsive proteins, we determined the protein expression profile of the serum after docetaxel infusion using a surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI TOF-MS) system.


METHODS
Blood from breast cancer patients was collected before and 4, 8, 24 and 48 hours after docetaxel infusion. The protein expression profile was determined by a SELDI TOF-MS system. The relative expression levels of target proteins were compared during the time-course after docetaxel injection.


RESULTS
We identified two representative proteins with molecular weights of 7790 Da and 9285 Da. The 7790 Da protein was high molecular weight kininogen, and the 9285 Da protein was apolipoprotein A-II. These two proteins had similar expression patterns in 5 patients, except one patient who experienced severe, acute, adverse effects.


CONCLUSIONS
These results suggest that protein expression profiles determined by SELDI TOF-MS represent useful data for the identification of treatment-responsive proteins.
Heike
Yuji
Y
Department of Medical Oncology and Clinical Research, National Shikoku Cancer Center, Matsuyama, Ehime 790-0007, Japan. yheike@ncc.go.jp
Hosokawa
Mami
M
Osumi
Shozo
S
Fujii
Daisuke
D
Aogi
Kenjiro
K
Takigawa
Nagio
N
Ida
Mikiko
M
Tajiri
Hisao
H
Eguchi
Kenji
K
Shiwa
Mieko
M
Wakatabe
Rumi
R
Arikuni
Hisashi
H
Takaue
Yoichi
Y
Takashima
Shigemitsu
S
D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000188:drug therapy; D000077143:Docetaxel; D005260:Female; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D043823:Taxoids / Q000009:adverse effects*
D015415:Biomarkers; D001798:Blood Proteins; D043823:Taxoids; D000077143:Docetaxel