title: Biological effect of irradiation on adhesion molecules in human colon cancer cells in vitro.
abstract: BACKGROUND
  Carbohydrate antigens, such as sialyl Lewis(a) antigen (s-Le(a)) and sialyl Lewis(x) antigen (s-Le(x)), play an important role in cancer metastasis in vitro and in vivo. Currently, preoperative radiotherapy is used to prevent local recurrence of rectal cancer. We investigated the effects of X-ray irradiation on the carbohydrate antigens s-Le(a) and s-Le(x) in vitro.
  
  
  METHODS
  The cell surface expressions of s-Le(a) and s-Le(x) were determined by flow cytometric analysis at 24 hours after X-ray irradiation of 4 human cancer cell lines. s-Le(a) and s-Le(x) functions were quantitated using a monolayer cell adhesion assay with human umbilical vein endothelial cells (HUVECs).
  
  
  RESULTS
  The cell surface expressions of s-Le(a) and s-Le(x) decreased at 24 hours after irradiation. s-Le(a) adhesion to HUVECs monolayers similarly decreased at 24 hours after irradiation.
  
  
  CONCLUSIONS
  These results may indicate a role for X-ray irradiation in the reduction of liver metastasis in patients with colon cancer.
authors.affiliation: Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hasetake@med.nagoya-cu.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Mikinori
authors.initials: M
authors.lastname: Sato
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kurimoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawashima
authors.forename: Youichi
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Minoru
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hirozumi
authors.initials: H
authors.lastname: Sawai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Funahashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okada
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Takeyama
authors.forename: Tadao
authors.initials: T
authors.lastname: Manabe
mesh_terms: D018395:CA-19-9 Antigen; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy*; D042783:Endothelial Cells / Q000166:cytology; D005434:Flow Cytometry; D005732:Gangliosides / Q000096:biosynthesis / Q000502:physiology / Q000528:radiation effects*; D019073:HT29 Cells; D006801:Humans; D009844:Oligosaccharides / Q000096:biosynthesis / Q000502:physiology / Q000528:radiation effects*; D000080506:Sialyl Lewis X Antigen
chemical_list: D018395:CA-19-9 Antigen; D005732:Gangliosides; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C064564:sialyl Le(a) ganglioside