[Kinetic changes of plasma IL-1 and IL-6 levels in MPTP-induced Parkinson's disease model mice and their relationship with brain asymmetry].
OBJECTIVE
To set up Parkinson's disease (PD) model mice by using 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP), detect the levels of plasma IL-1 and IL-6, and to explore their relationship with brain asymmetry.


METHODS
Male C57BL/6J mice were divided into right pawed mouse group and left pawed mouse group according to paw preference test which reflects brain asymmetry. Mice were injected intraperitoneally with 25 mg/kg MPTP each day for 5 consecutive days. The mice were executed and blood samples were taken at 1, 3 and 14 days after the last time injection. Control mice were only injected with normal saline. The levels of plasma IL-6 and IL-1 were detected by ELISA.


RESULTS
Plasma IL-6 level in normal control mice was low, but elevated dramatically at 14 days after last time injection of MPTP in PD model mice. Furthermore, IL-6 level of right pawed mice was higher than that of left pawed mice. Plasma IL-1 levels also increased on day 3 after last time injection of MPTP, and the level of left pawed mice was higher than that of right pawed mice.


CONCLUSIONS
IL-6 and IL-1 probably participate in the occurrence and progress of MPTP-induced PD in model mice, and were related with brain asymmetry.
Shen
Yan-qin
YQ
Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515031, China.
Li
Kang-sheng
KS
Neveu
P-J
PJ
D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D007839:Functional Laterality; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D007700:Kinetics; D020267:MPTP Poisoning / Q000097:blood* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010300:Parkinson Disease / Q000097:blood* / Q000503:physiopathology*
D007375:Interleukin-1; D015850:Interleukin-6