title: Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif.
abstract: The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3'-end of the intergenic hairpin, i.e. position 1568-1574 for N and position 1852-1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3'-end. The potential involvement of the 3'-end structure in transcription termination is discussed.
authors.affiliation: Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709PD Wageningen, The Netherlands.
authors.forename: Ingeborg
authors.initials: I
authors.lastname: van Knippenberg
authors.forename: Rob
authors.initials: R
authors.lastname: Goldbach
authors.forename: Richard
authors.initials: R
authors.lastname: Kormelink
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017865:Tospovirus / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral