title: Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture.
abstract: Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1 and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d 1 was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation.
authors.affiliation: Department of Public Health, Nara Medical University, Kashihara City, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Dohi
authors.forename: Hideki
authors.initials: H
authors.lastname: Shimaoka
authors.forename: Masako
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.forename: Kunio
authors.initials: K
authors.lastname: Yonemasu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Minami
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001846:Bone Development / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D015151:Immunoblotting; D007536:Isomerism; D008297:Male; D008648:Mesoderm / Q000166:cytology; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000893:Anti-Inflammatory Agents; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012313:RNA; D003907:Dexamethasone; D008668:Metallothionein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000469:Alkaline Phosphatase; D002118:Calcium