title: Conversion of Staphylococcus epidermidis strains from commensal to invasive by expression of the ica locus encoding production of biofilm exopolysaccharide.
abstract: To test if biofilm formation in Staphylococcus epidermidis is dependent on the polysaccharide intercellular adhesin, whose biosynthesis is driven by the ica locus, a plasmid containing the ica locus was transferred to three ica-negative strains. Using in vitro biofilm assays and a rat central venous catheter infection model, we confirmed the importance of the ica locus for biofilm production and pathogenesis of S. epidermidis.
authors.affiliation: Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Wen
authors.forename: Cuong
authors.initials: C
authors.lastname: Vuong
authors.forename: Michael
authors.initials: M
authors.lastname: Otto
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D002405:Catheterization, Central Venous / Q000009:adverse effects; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism*; D016459:Prosthesis-Related Infections / Q000382:microbiology; D051381:Rats; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D013290:Streptococcal Infections / Q000382:microbiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin