[Study on the interaction of anticancer drugs and DNA using fluorescence spectroscopy].
Fluorescence spectroscopy has achieved great successes in the study on the interaction of drugs and DNA in both quantitative analysis and qualitative analysis. While the fluorescence probe technique can be used to measure the intensity of drugs-DNA interaction, because the injected drugs can change the fluorescence intensity of the probe. In this paper, the authors use berberine as a probe to measure the interaction intensity between some anticancer drugs and DNA, calculate the constant D and determine the effects of those drugs. In addition, the authors point out that this method can not be efficient for certain drug solution.
Shen
Jing-shan
JS
School of Physics, Peking University, Beijing 100871, China.
Sun
Dan-dan
DD
Fu
Lian-chun
LC
Lü
Wen-bo
WB
Lü
Guo-wei
GW
Ye
Xue-min
XM
Meng
Guang-zheng
GZ
Song
Zeng-fu
ZF
D000970:Antineoplastic Agents / Q000737:chemistry*; D001599:Berberine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005472:Fluorouracil / Q000737:chemistry; D007700:Kinetics; D008937:Mitomycins / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000970:Antineoplastic Agents; D008937:Mitomycins; D001599:Berberine; D004247:DNA; D005472:Fluorouracil