title: Quantitative analysis of the marker compounds in Salvia miltiorrihiza root and its phytomedicinal preparations.
abstract: A HPLC method is described in order to separate and identify 4 phenolic compounds including lithospermic acid B, 3,4-dihydroxyphenyllactic acid (danshensu), rosmarinic acid, and protocatechuic aldehyde as well as 3 lipophilic ones (tanshinone I, tanshinone II(A) and cryptotanshinone) from the roots of Salvia miltiorrihiza or the herbal product containing S. miltiorrihiza available in Chinese or Japanese market. The influence of extractive conditions, such as method, solvents and time, on the 4 phenolic marker components in S. miltiorrihiza is well discussed. In order to investigate the stability of lithospermic acid B, the preparation samples of an injection and a granule were analyzed after being stored at 4 degrees C, 20 degrees C and 40 degrees C for up to six months.
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
authors.forename: Ying-ni
authors.initials: YN
authors.lastname: Pan
authors.forename: Wen-wei
authors.initials: WW
authors.lastname: Fu
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Makino
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kano
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D008517:Phytotherapy; D018517:Plant Roots; D010946:Plants, Medicinal; D027561:Salvia miltiorrhiza
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal