Identification and function of a shrimp white spot syndrome virus (WSSV) gene that encodes a dUTPase.
The ORF wsv112 of shrimp white spot syndrome virus (WSSV) was predicted to encode a protein with five conserved motifs at its N-terminus characteristics of dUTPases. The transcription of the gene named as wdut was analyzed by RT-PCR and RACE. The C-terminal end of the putative WSSV dUTPase bore very low similarity to the reported dUTPases and any other known proteins. Therefore, the 5'-terminal region (528-bp) of wdut gene was expressed in E. coli. The recombinant WSSV dUTPase (WDUT) with a molecular mass of 23 kDa could catalyze the hydrolysis of dUTP into dUMP and was highly specific for dUTP with an apparent Km of 1.2 microM. Furthermore, gel filtration results revealed that this enzyme was a trimer.
Liu
Xiaoqing
X
Key Laboratory of Marine Biogenetic Resources (SOA and FJ), Third Institute of Oceanography, 178 Daxue Rd., Xiamen 361005, PR China.
Yang
Feng
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D021122:Protein Subunits; D011755:Pyrophosphatases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D014158:Transcription, Genetic; D046848:White spot syndrome virus 1 / Q000201:enzymology / Q000235:genetics*
D003856:Deoxyuracil Nucleotides; D021122:Protein Subunits; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; C027078:deoxyuridine triphosphate; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase