title: Gene expression profiling in hypothalamus of immobilization-stressed mouse using cDNA microarray.
abstract: To investigate the effects of repeated immobilization-stress challenge on HPA axis, genomic transcriptome in the hypothalamus of immobilization-stressed mouse was analyzed by using cDNA microarray. With the 1.5-fold cutoff of arbitrary criteria, the expression levels of 108 genes out of 6016 genes were significantly modulated in the hypothalamus by the stress. Energy metabolism-, lipid metabolism-, and apoptosis- and signal transduction-related genes were activated while DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in the hypothalamus. Eighteen genes among them were selected for RT-PCR analysis to confirm the change of their expression levels on agarose gels. Besides, dozens of novel genes, which have not been previously reported, were screened to be modulated by the immobilization stress through the transcriptome analysis. These genes are related to apoptosis, tumor-suppression, DNA-binding and protein folding, and thus may be used as potential targets for the development of therapeutics of chronic stress or depressant.
authors.affiliation: Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, Korea.
authors.forename: Han-Chang
authors.initials: HC
authors.lastname: Lee
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Chang
authors.forename: Mijung
authors.initials: M
authors.lastname: Yeom
authors.forename: Gun-Ho
authors.initials: GH
authors.lastname: Kim
authors.forename: Kang-Duk
authors.initials: KD
authors.lastname: Choi
authors.forename: Insop
authors.initials: I
authors.lastname: Shim
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Hahm
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D012149:Restraint, Physical / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
chemical_list: D012333:RNA, Messenger