title: ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3.
abstract: Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3zeta and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.
authors.affiliation: The State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. pzhu@cbr.med.harvard.edu
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Zhu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Sang
authors.forename: Huanbing
authors.initials: H
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Rener
authors.initials: R
authors.lastname: Xu
authors.forename: Yubo
authors.initials: Y
authors.lastname: Sun
authors.forename: Tianhong
authors.initials: T
authors.lastname: Xu
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.forename: Linfeng
authors.initials: L
authors.lastname: Chen
authors.forename: Hanping
authors.initials: H
authors.lastname: Feng
authors.forename: Changben
authors.initials: C
authors.lastname: Li
authors.forename: Shouyuan
authors.initials: S
authors.lastname: Zhao
mesh_terms: D048948:14-3-3 Proteins / Q000502:physiology*; D051722:ADAM Proteins; D001665:Binding Sites; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D051722:ADAM Proteins; C498474:ADAM22 protein, human