title: Development of a rapid autopsy program for studies of brain immunity.
abstract: Human glia are essential cellular models used for studies of neurodegenerative diseases. Fetal neuroglia are commonly used, as they can be recovered in large quantities and sustained for long periods in culture. However, fetal neuroglia may have limitations in reflecting adult diseases and additionally can pose ethical issues in translating products of abortion for research use. To address these concerns, we developed a rapid autopsy program to procure age- and disease-specific neuroglia from adult brain tissues within hours of death. The challenges in developing this initiative, reflecting experiences from 69 autopsies over 4 years, are presented.
authors.affiliation: Laboratory of Cellular Neuroimmunology, 985215 Nebraska Medical Center, Omaha, NE, 68198-5215, USA. aghorpad@unmc.edu
authors.forename: Anuja
authors.initials: A
authors.lastname: Ghorpade
authors.forename: Leslie
authors.initials: L
authors.lastname: Bruch
authors.forename: Yuri
authors.initials: Y
authors.lastname: Persidsky
authors.forename: Betty
authors.initials: B
authors.lastname: Chin
authors.forename: William H C
authors.initials: WH
authors.lastname: Brown
authors.forename: Kathleen
authors.initials: K
authors.lastname: Borgmann
authors.forename: Raisa
authors.initials: R
authors.lastname: Persidsky
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Spring
authors.initials: S
authors.lastname: Holter
authors.forename: Robin
authors.initials: R
authors.lastname: Cotter
authors.forename: Jill
authors.initials: J
authors.lastname: Faraci
authors.forename: David
authors.initials: D
authors.lastname: Heilman
authors.forename: VaKara
authors.initials: V
authors.lastname: Meyer
authors.forename: Jane F
authors.initials: JF
authors.lastname: Potter
authors.forename: Susan
authors.initials: S
authors.lastname: Swindells
authors.forename: Howard E
authors.initials: HE
authors.lastname: Gendelman
mesh_terms: D000046:Academic Medical Centers / Q000379:methods; D000328:Adult; D001344:Autopsy / Q000379:methods*; D001921:Brain / Q000276:immunology* / Q000473:pathology*; D002478:Cells, Cultured; D003159:Community-Institutional Relations; D006801:Humans; D007111:Immunity, Cellular; D009457:Neuroglia / Q000276:immunology / Q000473:pathology; D016730:Program Development / Q000379:methods*; D013997:Time Factors; D014015:Tissue Banks / Q000458:organization & administration; D009927:Tissue and Organ Procurement