Simultaneous determination of 8-hydroxydeoyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine.
Recently, H. Kasai reported an automatic, precise method of 8-hydroxydeoxyguanosine (8-OH-dG) analysis in urine by high performance liquid chromatography coupled to an electrochemical detector (HPLC-ECD). It is based on a cleaning-up step by anion-exchange chromatography and a further purification step using reverse phase chromatography before detection, by the ECD. In this communication, we report a method for the simultaneous determination of 8-OH-dG and creatinine, an internal standard for normalizing the excretion of 8-OH-dG in urine.
Kasai
Hiroshi
H
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Svoboda
Peter
P
Yamasaki
Sayumi
S
Kawai
Kazuaki
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004784:Environmental Monitoring; D006801:Humans; D018384:Oxidative Stress; D012015:Reference Standards
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine