Mycobacterium florentinum sp. nov., isolated from humans.
Eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were Mycobacterium triplex and Mycobacterium lentiflavum. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of hsp65. The strains presented high resistance to antimycobacterial drugs. The name Mycobacterium florentinum sp. nov. is proposed for this taxon, with strain FI-93171(T) (=DSM 44852(T) = CIP 108409(T)) as the type strain.
Tortoli
Enrico
E
Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
Rindi
Laura
L
Department of Experimental Pathology, Medical Biotechnologies, Infectivology and Epidemiology, University of Pisa, 56127 Pisa, Italy.
Goh
Khye S
KS
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, 97165 Pointe-à-Pitre, Guadeloupe, France.
Katila
Marja L
ML
Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland.
Mariottini
Alessandro
A
Genetics and Cytogenetics Unit, Careggi Hospital, 50134 Florence, Italy.|Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
Mattei
Romano
R
Clinical Laboratory, Campo di Marte Hospital, 55100 Lucca, Italy.
Mazzarelli
Gianna
G
Microbiological and Virological Serum-immunology Laboratory, Careggi Hospital, 50134 Florence, Italy.|Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
Suomalainen
Sini
S
Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland.
Torkko
Pirjo
P
Laboratory of Environmental Microbiology, National Public Health Institute, 70211 Kuopio, Finland.|Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland.
Rastogi
Nalin
N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, 97165 Pointe-à-Pitre, Guadeloupe, France.
D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D002648:Child; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D008198:Lymph Nodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins