[New advances of high performance separation and analytical techniques for proteins in complex biological systems].
As a new strategy, proteomics is playing more and more important roles in the life sciences after the sequencing of the human genome was completed. Because a biological system is very complex and diverse, the study and application of technological platform for the separation and analysis with high resolution, high sensitivity, high throughput and broad dynamic range have become one of the important and hot spots. The review focused on the new progress of high performance separation and analysis techniques which were applied to the field of proteomics increasingly, such as multidimensional liquid chromatography for pre-fractionation, capillary liquid chromatography coupled with mass spectrometry and capillary electrophoresis interfaced on line with mass spectrometry.
Meng
Qingfang
Q
Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China.
Zhang
Yangjun
Y
Wang
Jinglan
J
Cai
Yun
Y
Qian
Xiaohong
X
D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid / Q000145:classification / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*
D011506:Proteins