title: Collagen XVII and BPAG1 expression in the retina: evidence for an anchoring complex in the central nervous system.
abstract: The ectoderm gives rise not only to the skin but also to the entire CNS. This common embryonic lineage suggests that some molecular isoforms might serve analogous functions in both tissues. Indeed, not only are laminins important components of dermal adhesion mechanisms, but they also regulate some aspects of synaptic development in both the CNS and the PNS. In the skin, laminins are part of a hemidesmosome complex essential for basal keratinocyte adhesion that includes collagen XVII (BP180) and BPAG1 (dystonin/BP230). Here, we show that CNS neurons also express collagen XVII and BPAG1 and that these molecules are expressed in the adult and developing retina. In the retina, isoforms of collagen XVII and BPAG1 are colocalized with laminins at photoreceptor synapses and around photoreceptor outer segments; both molecules are expressed by rods, whereas cones express collagen XVII but not BPAG1. Moreover, biochemical data demonstrate that collagen XVII complexes with retinal laminins. We propose that collagen XVII and BPAG1 isoforms may help to anchor elements of the rod photoreceptor cytomatrix to the extracellular matrix.
authors.affiliation: Department of Anatomy and Cellular Biology, Tufts University School of Medicine, and the Tufts Center for Vision Research, Boston, Massachusetts 02111, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Claudepierre
authors.forename: Mary K
authors.initials: MK
authors.lastname: Manglapus
authors.forename: Nathan
authors.initials: N
authors.lastname: Marengi
authors.forename: Stephanie
authors.initials: S
authors.lastname: Radner
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Champliaud
authors.forename: Kaisa
authors.initials: K
authors.lastname: Tasanen
authors.forename: Leena
authors.initials: L
authors.lastname: Bruckner-Tuderman
authors.forename: Dale D
authors.initials: DD
authors.lastname: Hunter
authors.forename: William J
authors.initials: WJ
authors.lastname: Brunken
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism*; D017364:Collagenases / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D016547:Kinesins; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D009124:Muscle Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D024041:Non-Fibrillar Collagens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism; D011257:Pregnancy Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism; D000097805:Collagen Type XVII
chemical_list: D016282:Aspartic Acid Endopeptidases; D001324:Autoantigens; D002135:Calcium-Binding Proteins; D017364:Collagenases; D016547:Kinesins; D007797:Laminin; D008562:Membrane Glycoproteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D019887:Peanut Agglutinin; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D014746:Vimentin; D000097805:Collagen Type XVII; C033652:postsynaptic density proteins; C058783:laminin beta2; C074152:kinesin-II; C094766:pregnancy-associated glycoprotein 1