Immunocytochemical and phylogenetic distribution of aquaporins in the frog ventral skin and urinary bladder.
We recently cloned three cDNAs encoding frog aquaporin (AQP-h1, BAC07470; AQP-h2, BAC82379; and AQP-h3, BAC07471) from the ventral pelvic skin of the tree frog, Hyla japonica. The present study demonstrated that Hyla AQP-h2 was translocated from cytoplasmic pools to the apical plasma membranes of the granular cells in the bladder after antidiuretic hormone stimulation and that Hyla AQP-h2 and AQP-h3 behaved similarly in the ventral pelvic skin. Further, we found that terrestrial and tree frogs, but not aquatic and semiterrestrial-adapted frogs, absorbed water from their ventral pelvic skin by AQP-h3-like protein in concert with AQP-h2-like protein.
Tanaka
S
S
Department of Biology, Faculty of Science, Shizuoka University, Japan. sbstana@ipc.shizuoka.ac.jp
Hasegawa
T
T
Tanii
H
H
Suzuki
M
M
D000818:Animals; D001001:Anura; D051398:Aquaporin 1 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051399:Aquaporin 2 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D051400:Aquaporin 3 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D020346:Aquaporins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D002025:Bufonidae; D007150:Immunohistochemistry; D010802:Phylogeny; D011892:Rana catesbeiana; D012867:Skin / Q000737:chemistry / Q000378:metabolism*; D001743:Urinary Bladder / Q000737:chemistry / Q000378:metabolism*; D014982:Xenopus laevis
D051399:Aquaporin 2; D020346:Aquaporins; D051398:Aquaporin 1; D051400:Aquaporin 3