title: Increased expression of ICAM-3 is associated with radiation resistance in cervical cancer.
abstract: To search for a marker that predicts the efficacy of radiation therapy in human cervical cancer, gene expression profiles between parental SiHa cervical cancer cells and radiation-resistant SiHa/R cells have been compared by the microarray technique. Microarray and Northern blot analyses demonstrated that the ICAM-3 expression was upregulated in SiHa/R cells. This increased expression of ICAM-3 in SiHa cells enhanced cell survival by about 34.3% after a 2 Gy dosage of radiation. In addition, SiHa/ICAM-3 cells showed a 2.45-fold higher level of FAK phosphorylation than that of the control cells. In tumor specimens, ICAM-3 staining was restricted to tumor stromal endothelial cells and lymphocytes. The overexpression of ICAM-3 was significantly more frequent in radiation-resistant cervical cancer specimens when compared with radiation-sensitive specimens (83.3% vs. 35.3%; p = 0.015). With these observations, we can suggest that an increased expression of ICAM-3 is associated with radiation resistance in cervical cancer cells and the expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy.
authors.affiliation: Department of Internal Medicine and Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, South Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Chung
authors.forename: Byoung-Gie
authors.initials: BG
authors.lastname: Kim
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Park
authors.forename: Seung Jae
authors.initials: SJ
authors.lastname: Huh
authors.forename: Jhingook
authors.initials: J
authors.lastname: Kim
authors.forename: Jong Kuk
authors.initials: JK
authors.lastname: Park
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Cho
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Jun Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Young Do
authors.initials: YD
authors.lastname: Yoo
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Bae
mesh_terms: D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000166:cytology / Q000276:immunology; D003114:Colony-Forming Units Assay; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms; D008175:Lung Neoplasms; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000532:radiotherapy
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D017931:DNA Primers; C483616:ICAM3 protein, human; D034741:RNA, Small Interfering