Quantification of tissue inhibitor of metalloproteinases 2 in plasma from healthy donors and cancer patients.
Tissue inhibitor of metalloproteinases (TIMP)-2 is a highly conserved molecule, which binds both active and latent matrix metalloproteinase (MMP)-2. TIMP-2 is also involved in the activation of MMP-2 on the cell surface. A quantitative enzyme-linked immunosorbent assay (ELISA) was established and optimized for measurement of TIMP-2 in plasma. The capturing antibody in the ELISA was a monoclonal, while the detecting antibody was a chicken polyclonal antibody recognizing the native form of human TIMP-2. The levels of TIMP-2 were measured in ethylenediaminetetraacetic acid (EDTA) and citrate plasma from healthy donors. The median values were determined as 163 ng/ml (n = 186) with a range of 109-253 ng/ml for EDTA plasma and 139 ng/ml (n = 77) with a range of 95-223 ng/ml for citrate plasma. The TIMP-2 concentration in citrate plasma from 15 patients with advanced, stage IV breast cancer had a median value of 160 ng/ml, only slightly higher but statistically distinguishable from the level found in citrate plasma from the healthy donors. In addition, the TIMP-2 concentration in EDTA plasma from colorectal cancer patients revealed a significantly higher level in plasma from patients with Dukes stage A (P = 0.01) compared with patients with more advanced Dukes stages.
Larsen
M B
MB
The Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
Stephens
R W
RW
Brünner
N
N
Nielsen
H J
HJ
Engelholm
L H
LH
Christensen
I J
IJ
Stetler-Stevenson
W G
WG
Høyer-Hansen
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000097:blood; D001782:Blood Donors; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology; D002951:Citrates / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000097:blood; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D010949:Plasma / Q000187:drug effects; D020539:Sequence Analysis, Protein; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood* / Q000737:chemistry
D014408:Biomarkers, Tumor; D002951:Citrates; D019716:Tissue Inhibitor of Metalloproteinase-2; D004492:Edetic Acid