title: Protein-functionalized polymer brushes.
abstract: A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.
authors.affiliation: Laboratoire des Polymères, Institut des Matériaux, Bâtiment MX-D, and Laboratoire d'Ingénierie des Protéines, Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Stefano
authors.initials: S
authors.lastname: Tugulu
authors.forename: Anke
authors.initials: A
authors.lastname: Arnold
authors.forename: India
authors.initials: I
authors.lastname: Sielaff
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
authors.forename: Harm-Anton
authors.initials: HA
authors.lastname: Klok
mesh_terms: D011108:Polymers / Q000138:chemical synthesis*; D011485:Protein Binding; D011506:Proteins / Q000138:chemical synthesis*
chemical_list: D011108:Polymers; D011506:Proteins