title: Evolution of T lymphocytes and cytokine expression in classical swine fever (CSF) virus infection.
abstract: This study characterized the cell-mediated immune response in pigs inoculated with the Alfort 187 isolate of classical swine fever (CSF) virus. Quantitative changes in the T-lymphocyte population (CD3(+), CD4(+) and CD8(+)) and qualitative changes in cytokine expression (IL-2, IL-4 and IFNgamma) by these cells in serum, thymus and spleen were demonstrated. These changes coincided spatially and temporally with previously described quantitative and qualitative changes in monocyte-macrophage populations, thus demonstrating the contribution of the two cell populations to lymphoid depletion. Moreover, examination of cytokine expression in thymus and spleen samples revealed a type 1 cell-mediated immune response in the early and middle stages of the experiment, giving way to a type 2 immune response towards the end of the experiment; these findings, which accorded with the serological results and lymphopenia, may influence the delayed humoral response characteristic of CSF.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Edificio de Sanidad Animal, Campus de Rabanales, 14014 Córdoba, Spain.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: A
authors.initials: A
authors.lastname: Núñez
authors.forename: F J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D002452:Cell Count; D006691:Classical Swine Fever / Q000276:immunology* / Q000378:metabolism; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D013154:Spleen / Q000276:immunology / Q000473:pathology; D013552:Swine; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma