Expression of novel organic cation/carnitine transporter (OCTN2) in the mouse pancreas.
Among the organic cation transporters, OCTN2 is identified as the most important carnitine transporter owing to the ability to transport carnitine. Although the OCTN2 is previously found in various tissues, there have been no reports showing the OCTN2 in the pancreas. In this study, we examined the expression and localization of OCTN2 in the mouse pancreas by the aid of an in situ hybridization technique and immunohistochemistry with anti-OCTN2 antibody. As a result, the OCTN2 expression was found in the A-cells for the first time. OCTN2 was not expressed in B-cells, notwithstanding that the metabolism of long-chain fatty acids, which are transported into the mitochondria with the help of carnitine, was expected for fatty acid-stimulated insulin secretion. Thus, this study suggests the possibility of carnitine uptake in the pancreatic A-cells through OCTN2 and implies the presence of carnitine transporter(s) other than OCTN2 in the B-cell.
Kai
Saori
S
Department of Urology, Miyazaki Medical College Graduate School of Medicine, Kiyotake, Miyazaki 889-1692, Japan.
Yakushiji
Kazumichi
K
Yamauchi
Masamichi
M
Ito
Chizuru
C
Kuwajima
Masamichi
M
Osada
Yukio
Y
Toshimori
Kiyotaka
K
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009928:Organ Specificity; D027701:Organic Cation Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000074058:Solute Carrier Family 22 Member 5
D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; C492019:Slc22a5 protein, mouse; D000074058:Solute Carrier Family 22 Member 5