[Detecting haplotypes of four Y-STR by multiplex amplification].
OBJECTIVE
To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.


METHODS
DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.


RESULTS
When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.


CONCLUSIONS
The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.
Liu
Qiu-ling
QL
Faulty of Forensic Medicine, Zhong shan Medical College, Zhong shan University, Guangzhou 510089, China.
Lu
De-jian
DJ
Xu
Ni-Mei
NM
D000483:Alleles; D002681:China; D041322:Chromosomes, Human, Y; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016622:Silver Staining; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004247:DNA