Basement membrane composition in the early mouse embryo day 7.
Basement membranes (BM) are specialized structures of the extracellular matrix known to be involved in various early developmental processes. Despite numerous investigations on the localization of BM components, it remains unknown which molecules are expressed in early developmental stages and by which germ layers these proteins are produced. Therefore, we tested for all known laminin chains, nidogens, collagen type IV, and perlecan by means of light microscopic immunostaining and performed in situ reverse transcriptase-polymerase chain reaction to detect the mRNAs specific for laminin alpha1, laminin beta1, the alpha1 chain of collagen type IV, nidogen-2, and perlecan in the early mouse embryo, day 7, in vivo. Only the laminin chains alpha1, beta1, and gamma1 were detected immunohistochemically throughout the entire endodermal and ectodermal BM zones of the embryo proper. The mRNA of laminin alpha1, laminin beta1, collagen type IV, nidogen-2 and perlecan were expressed in the ectoderm-derived mesoderm, in the endoderm as well as in the ectoderm. In contrast, Reichert's membrane was positive for all laminin chains except for the alpha4, alpha5, beta3, and gamma3 chains. Moreover, maternal epithelial as well as mesenchymal cells expressed laminins, nidogen-1 and nidogen-2, collagen type IV, and perlecan. In conclusion, laminin-1 might be the only laminin isoform in the early mouse embryo that, together with the other main BM components, nidogens, collagen type IV, and perlecan, is synthesized by all three germ layers.
Gersdorff
Nikolaus
N
Department of Prosthodontics, Georg-August-Universität Göttingen, Göttingen, Germany.
Müller
Matthias
M
Otto
Sascha
S
Poschadel
Ralf
R
Hübner
Stephan
S
Miosge
Nicolai
N
D000818:Animals; D001485:Basement Membrane / Q000196:embryology* / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009035:Mothers; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D007797:Laminin; D008562:Membrane Glycoproteins; C112846:Nid2 protein, mouse; D012333:RNA, Messenger; C029967:nidogen; C071927:perlecan