title: Nanogel-quantum dot hybrid nanoparticles for live cell imaging.
abstract: We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Urara
authors.initials: U
authors.lastname: Hasegawa
authors.forename: Shin-ichiro M
authors.initials: SM
authors.lastname: Nomura
authors.forename: Sunil C
authors.initials: SC
authors.lastname: Kaul
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D002240:Carbohydrate Sequence; D002477:Cells; D002784:Cholesterol / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D008969:Molecular Sequence Data; D036103:Nanotechnology; D010316:Particle Size
chemical_list: D005936:Glucans; C009109:pullulan; D002784:Cholesterol