[Rapid extraction of MtDNA from Cryptococcus neoformans and examined by electronic microscopy].
Cryptococcus neoformans may be grown to the exponential phase,are broken by a combination of NovoZym234 and mechanical means,and mitochondrial DNA was extracted from DNaseI-treated mitochondrial preparation by differential centrifugation. Three pellets, including yeast cell, protoplasts, mitochondrial, were examined by transmission electronic microscopy. The resulting mtDNA is sufficiently pure for restriction endonucleases analysis and PCR in further studying. A rapid and effective method for the preparation of the mtDNA of C.neoformans was established.
Zou
Xian-biao
XB
Department of Dermatology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China. xbzou@sina.com
Liao
Wan-qing
WQ
Wen
Hai
H
Wu
Jian-hua
JH
Qiu
Yun
Y
Yang
Jin-shui
JS