title: Renin-angiotensin system polymorphisms and coronary artery surgery patients.
abstract: The frequencies of angiotensin-converting enzyme gene insertion/deletion, angiotensinogen-M253T, and angiotensin II type 1 receptor-A1166C polymorphisms were analyzed in 105 patients undergoing coronary artery bypass grafting (group 1) and a control group of 105 non-cardiac patients (group 2). Blood samples were obtained for biochemical analyses and DNA extraction. Genotyping was performed by polymerase-chain-reaction-based restriction analysis. According to the angiotensin-converting enzyme gene insertion/deletion polymorphism, 36.3% of patients in group 1 and 30.7% in group 2 were homozygous for the DD allele. This difference was not statistically significant. Angiotensin II type 1 receptor-A1166C genotype polymorphism was also not significantly different between the groups. The results showed the angiotensinogen-M235T polymorphism to be heterogenous. The MM homozygote frequency was significantly higher in controls (72.3%), whereas 80% of the TT homozygote frequency was in the surgical group ( p = 0.001). These results show that although there were no significant differences in angiotensin-converting enzyme gene insertion/deletion and angiotensin II type 1 receptor-A1166C genotype polymorphisms between the groups, angiotensinogen-M235T polymorphism of TT homozygote frequency was significantly associated with patients undergoing coronary artery bypass surgery.
authors.affiliation: Department of Cardiovascular Surgery, Ankara Namune Education and Research Hospital, Metis Biotechnology Ltd., Ankara, Turkey. sozisik2002@yahoo.com
authors.forename: Kanat
authors.initials: K
authors.lastname: Ozisik
authors.forename: Muge
authors.initials: M
authors.lastname: Misirlioglu
authors.forename: Tulga A
authors.initials: TA
authors.lastname: Ulus
authors.forename: Serdar
authors.initials: S
authors.lastname: Tuncer
authors.forename: Mustafa
authors.initials: M
authors.lastname: Emir
authors.forename: Fehmi
authors.initials: F
authors.lastname: Katircioglu
mesh_terms: D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000235:genetics* / Q000601:surgery; D004251:DNA Transposable Elements; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen