title: Induction of gastric GIST in rat and establishment of GIST cell line.
abstract: Continuous administration of the direct-reacting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine reportedly produces not only adenocarcinoma in rats, but also mesenchymal tumors. A large number of tumors diagnosed as gastrointestinal smooth muscle tumors actually represent gastrointestinal stromal tumors (GISTs) in human cases. We have induced mesenchymal tumors by duodenal reflux in rats. To clarify the differentiation of these mesenchymal tumors, immunohistochemical investigations were undertaken. In addition, the first culture model of GIST-DR derived from GIST induced by duodenal reflux was established. GIST-DR cells, both in vitro and in vivo, were strongly immunopositive for both KIT and CD34, and STI571 (Imatinib mesylate) blocked the proliferation of this cell line. The present results suggest that duodenal reflux plays a role in the histogenesis of GIST.
authors.affiliation: Department of Pathology, Shiga University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga 520-2192, Japan. mukaisho@belle.shiga-med.ac.jp
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Mukaisho
authors.forename: Koichi
authors.initials: K
authors.lastname: Miwa
authors.forename: Yukari
authors.initials: Y
authors.lastname: Totsuka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Shimomura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugihara
authors.forename: Keiji
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Takanori
authors.initials: T
authors.lastname: Hattori
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D001549:Benzamides; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004383:Duodenogastric Reflux; D046152:Gastrointestinal Stromal Tumors / Q000378:metabolism / Q000473:pathology*; D000068877:Imatinib Mesylate; D066298:In Vitro Techniques; D008297:Male; D008637:Mesenchymoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010879:Piperazines / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018952:Antigens, CD34; D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D019009:Proto-Oncogene Proteins c-kit