[Applications of two-dimensional liquid chromatography coupled to mass spectrometry for the separation and identification of compounds in ginkgo biloba extracts].
A comprehensive two-dimensional liquid chromatographic separation system based on the combination of an immobilized liposome chromatographic (ILC) column and an ODS column was developed for the separation of components in Ginkgo biloba, a traditional Chinese medicine. Two columns were coupled by a two-position, eight-port valve equipped with two storage loops, and the system was controlled by a computer. The effluent was detected both by a diode array detector and by an atmospheric pressure chemical ionization (APCI) mass spectrometer. Under the optimization separation conditions with the separation system, more than 41 components in the methanol extract of Ginkgo biloba were resolved. According to their UV and mass spectra, 13 of them were preliminarily identified as ginkgolide B, ginkgolide C, bilobalide, rutin, quercetin, quercetin-3-O-beta-D-glucosyl (1-2)-alpha-L-rhamnoside, quercetin-3-O-beta-D-glucoside, isorhamnetin, kaempferol-3-O-beta-D-glucosyl (1-2)-alpha-L-rhamnoside, isohamnetin-3-O-beta-D-rutinoside, kaempferol-3-O-beta-D-glucoside, kaempferol, kaempferol-3-O-beta-D-rutinoside.
Chen
Xueguo
X
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China.
Kong
Liang
L
Sheng
Lianghong
L
Li
Xin
X
Zou
Hanfa
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003517:Cyclopentanes / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D044948:Flavonols / Q000032:analysis; D005663:Furans / Q000032:analysis; D020441:Ginkgo biloba; D046934:Ginkgolides / Q000032:analysis* / Q000737:chemistry; D044949:Kaempferols / Q000032:analysis; D007783:Lactones / Q000032:analysis; D008516:Medicine, Chinese Traditional; D009005:Monosaccharides / Q000032:analysis; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D012431:Rutin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D003517:Cyclopentanes; D004365:Drugs, Chinese Herbal; D044948:Flavonols; D005663:Furans; D046934:Ginkgolides; D044949:Kaempferols; D007783:Lactones; D009005:Monosaccharides; C511963:kaempferol-3-O-glucoside; C047368:3-methylquercetin; C016527:isoquercitrin; C058295:ginkgolide C; C492687:kaempferol-3-O-rutinoside; D012431:Rutin; C006552:kaempferol; D011794:Quercetin; C045856:ginkgolide B; C073710:bilobalide