title: Tubulin polyglutamylase enzymes are members of the TTL domain protein family.
abstract: Polyglutamylation of tubulin has been implicated in several functions of microtubules, but the identification of the responsible enzyme(s) has been challenging. We found that the neuronal tubulin polyglutamylase is a protein complex containing a tubulin tyrosine ligase-like (TTLL) protein, TTLL1. TTLL1 is a member of a large family of proteins with a TTL homology domain, whose members could catalyze ligations of diverse amino acids to tubulins or other substrates. In the model protist Tetrahymena thermophila, two conserved types of polyglutamylases were characterized that differ in substrate preference and subcellular localization.
authors.affiliation: Centre de Recherches de Biochimie Macromoléculaire, CNRS, 34293 Montpellier, France.
authors.forename: Carsten
authors.initials: C
authors.lastname: Janke
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Rogowski
authors.forename: Dorota
authors.initials: D
authors.lastname: Wloga
authors.forename: Catherine
authors.initials: C
authors.lastname: Regnard
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kajava
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Strub
authors.forename: Nevzat
authors.initials: N
authors.lastname: Temurak
authors.forename: Juliette
authors.initials: J
authors.lastname: van Dijk
authors.forename: Dominique
authors.initials: D
authors.lastname: Boucher
authors.forename: Alain
authors.initials: A
authors.lastname: van Dorsselaer
authors.forename: Swati
authors.initials: S
authors.lastname: Suryavanshi
authors.forename: Jacek
authors.initials: J
authors.lastname: Gaertig
authors.forename: Bernard
authors.initials: B
authors.lastname: Eddé
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D020134:Catalytic Domain; D002923:Cilia / Q000502:physiology; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009068:Movement; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013379:Substrate Specificity; D016808:Tetrahymena thermophila / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D011099:Polyglutamic Acid; D010453:Peptide Synthases; C112698:tubulin polyglutamylase