Abundance of ADAM-8, -9, -10, -12, -15 and -17 and ADAMTS-1 in mouse uterus during the oestrous cycle.
The aim of the present study was to determine whether a disintegrin and metalloproteinase (ADAM)-8, -9, -10, -12, -15 and -17 and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 are involved in the remodelling process of the mouse uterus during the oestrous cycle. The mRNA expression of ADAM was observed in all uterine tissues throughout the entire cycle. The levels of ADAM-8 mRNA were maximal at pro-oestrus, whereas the expression of ADAM-9 and ADAMTS-1 mRNA was maximal at oestrus. The minimum mRNA level of all ADAM genes always occurred at dioestrus. The mRNA levels of ADAM-10, -12, -15 and -17 did not vary significantly, regardless of the stage of the oestrous cycle. Immunoblot analyses demonstrated the presence of all ADAM proteins throughout the cycle. In terms of protein intensities, ADAM-8, -12 and -17 were maximal at pro-oestrus, whereas ADAM-10 and ADAMTS-1 were maximal at metoestrus and ADAM-9 was maximal at oestrus. Regardless of the ADAM species, minimal protein expression always occurred at dioestrus. Immunohistochemical studies showed ADAM protein expression in luminal and glandular epithelial layers, but not in the stromal layer. Moreover, ADAM proteins were found to be heterogeneously localised and their individual localisations depended on the stage of the oestrous cycle. From these observations, we suggest that the ADAM genes play an important role in mouse uterine tissue remodelling during the oestrous cycle.
Kim
Jiyoung
J
Department of Biotechnology, Seoul Women's University, Nowon-gu, Seoul, Korea.
Kim
Haekwon
H
Lee
Seung-Jae
SJ
Choi
Young Min
YM
Lee
Su-Jae
SJ
Lee
Joon Yeong
JY
D051722:ADAM Proteins / Q000032:analysis* / Q000235:genetics; D000072197:ADAM10 Protein; D000072199:ADAM12 Protein; D000072198:ADAM17 Protein; D000071097:ADAMTS1 Protein; D053829:Amyloid Precursor Protein Secretases; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000737:chemistry*
D015703:Antigens, CD; D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072199:ADAM12 Protein; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; C096115:Adam12 protein, mouse; C490902:Adam15 protein, mouse; C489402:Adam8 protein, mouse; C498316:Adam9 protein, mouse; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse; D000072198:ADAM17 Protein