title: Formulation and in vitro/in vivo evaluation of combining DNA repair and immune enhancing nutritional supplements.
abstract: Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifolafrondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic antioxidant competition yielding less than expected efficacious effects. Isobole curve analyses of these two active classes of ingredients determined by growth inhibition of HL-60 human leukemic cells in vitro confirmed they were indeed synergistic when in combination, and not metabolically competitive. Furthermore, an in vivo study showed significant health benefit for 14 subjects treated for 4 weeks with the unique C-Med-100/mushroom extract formulation in that they had reduced pain, reduced fatigue, weight loss and a reduced presence of DNA damage in peripheral blood assessed by (8-OH) guanine DNA adducts and elevation in serum protein thiols. Because this broad-based panel of clinical parameters indicating clinical efficacy has never been demonstrated before for either of the active ingredients evaluated alone in humans, these data were taken as strong evidence that the combination of C-Med-100 + mushroom extracts + nicotinamide + zinc gave additive or synergistic effects to health benefit, and thus supported no efficacious limits from metabolic competition regarding this particular formulation.
authors.affiliation: Department of Cell and Molecular Biology, Section for Tumor Immunology, University of Lund, Lund, Sweden. rwpero@attglobal.net
authors.forename: R W
authors.initials: RW
authors.lastname: Pero
authors.forename: A
authors.initials: A
authors.lastname: Amiri
authors.forename: Y
authors.initials: Y
authors.lastname: Sheng
authors.forename: M
authors.initials: M
authors.lastname: Welther
authors.forename: M
authors.initials: M
authors.lastname: Rich
mesh_terms: D000284:Administration, Oral; D000363:Agaricales; D018736:DNA Adducts / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D019587:Dietary Supplements; D004357:Drug Synergism; D005260:Female; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D009536:Niacinamide / Q000008:administration & dosage; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D032070:Uncaria; D015032:Zinc / Q000008:administration & dosage
chemical_list: C406667:C-MED-100; D018736:DNA Adducts; D010936:Plant Extracts; D009536:Niacinamide; D015032:Zinc