Patient-specific embryonic stem cells derived from human SCNT blastocysts.
Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated.
Hwang
Woo Suk
WS
College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. hwangws@snu.ac.kr
Roh
Sung Il
SI
Lee
Byeong Chun
BC
Kang
Sung Keun
SK
Kwon
Dae Kee
DK
Kim
Sue
S
Kim
Sun Jong
SJ
Park
Sun Woo
SW
Kwon
Hee Sun
HS
Lee
Chang Kyu
CK
Lee
Jung Bok
JB
Kim
Jin Mee
JM
Ahn
Curie
C
Paek
Sun Ha
SH
Chang
Sang Sik
SS
Koo
Jung Jin
JJ
Yoon
Hyun Soo
HS
Hwang
Jung Hye
JH
Hwang
Youn Young
YY
Park
Ye Soo
YS
Oh
Sun Kyung
SK
Kim
Hee Sun
HS
Park
Jong Hyuk
JH
Moon
Shin Yong
SY
Schatten
Gerald
G
D000328:Adult; D000361:Agammaglobulinemia; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D019976:Cloning, Organism; D016172:DNA Fingerprinting; D003922:Diabetes Mellitus, Type 1; D044127:Epigenesis, Genetic; D028744:Ethics Committees, Research; D005260:Female; D005347:Fibroblasts; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007258:Informed Consent; D007621:Karyotyping; D008297:Male; D053652:Nuclear Transfer Techniques; D018587:Oocyte Donation; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology; D013119:Spinal Cord Injuries; D033581:Stem Cell Transplantation; D009927:Tissue and Organ Procurement
D006680:HLA Antigens