The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves.
Nerve-mast cell interaction is involved in both homeostatic and pathologic regulations. The molecules that sustain this association have not been identified. Because synaptic cell adhesion molecule (SynCAM), alternatively named spermatogenic Ig superfamily (SgIGSF), is expressed on both nerves and mast cells and because it binds homophilically, this molecule may be a candidate. To examine this possibility, mast cells with or without SgIGSF/SynCAM were cocultured with superior cervical ganglion neurons that express SgIGSF/SynCAM, and the number of mast cells attached to neurites was counted. The attachment of mast cells with SgIGSF/SynCAM, i.e., bone marrow-derived mast cells (BMMC) from wild-type mice, was inhibited dose-dependently by blocking Ab to SgIGSF/SynCAM. Mast cells without SgIGSF/SynCAM, i.e., BMMC from microphthalmia transcription factor-deficient mice and BMMC-derived cell line IC-2 cells, were defective in attachment to neurite, and transfection with SgIGSF/SynCAM normalized this. When the nerves were specifically activated by scorpion venom, one-quarter of the attached IC-2 cells mobilized Ca(2+) after a few dozen seconds, and ectopic SgIGSF/SynCAM doubled this proportion. At points of contact between neurites and wild-type BMMC, SgIGSF/SynCAM was locally concentrated in both neurites and BMMC. SgIGSF/SynCAM on mast cells appeared to predominantly mediate attachment and promote communication with nerves.
Furuno
Tadahide
T
Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
Ito
Akihiko
A
Koma
Yu-Ichiro
Y
Watabe
Kenji
K
Yokozaki
Hiroshi
H
Bienenstock
John
J
Nakanishi
Mamoru
M
Kitamura
Yukihiko
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002450:Cell Communication / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D007136:Immunoglobulins / Q000502:physiology*; D008407:Mast Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D016501:Neurites / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000276:immunology / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology; D019089:Stem Cell Factor / Q000502:physiology; D017783:Superior Cervical Ganglion / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D025521:Tumor Suppressor Proteins
C498598:Cadm1 protein, mouse; C465040:Cadm1 protein, rat; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D007136:Immunoglobulins; D008565:Membrane Proteins; D010880:Piperidines; D019089:Stem Cell Factor; D025521:Tumor Suppressor Proteins; C080755:3-(2-methoxybenzylamino)-2-phenylpiperidine; D019009:Proto-Oncogene Proteins c-kit