title: Analysis of UNEP priority POPs using HRGC-HRMS and their contamination profiles in livers and eggs of great cormorants (Phalacrocorax carbo) from Japan.
abstract: The present investigation demonstrates establishment of United Nations Environment Programme (UNEP) priority Persistent Organic Pollutants (POPs) using high-resolution gas chromatography-high-resolution mass spectrometry. Particularly, POPs analytical methods were established using native and (13)C-labeled internal standards of HCHs, HCB, cyclodienes, chlordanes, DDTs, mirex, dioxin-like PCBs, PCDDs, and PCDFs by isotope dilution technique. The relative response factor for 6-point calibration curve native standards (18 replicate analysis) were in the ranges of 0.93-1.43 with relative standard deviation ranges from 1.68 to 4.96%. Instrument detection limit and instrument quantification limit was established for various POPs at femtograms. Concentrations of UNEP-POPs were measured in liver (n = 10) and egg (n = 10) of great cormorants and their major diet, gizzard shad (n = 2), collected in and around Tokyo, Japan. DDTs (ranges in liver and egg, respectively) were predominant accumulants (9800-310,000 and 9600-73,000) followed by dioxin-like PCBs (4500-69,000 and 7900-150,000), chlordanes (2600-16,000 and 700-4,800), cyclodienes (650-4600 and <1-1000), HCB (680-2800 and 180-590), HCHs (230-1800 and 120-490), PCDD/DFs (3.2-27 and 1.7-5.7) on nanogram per gram lipid basis. Concentrations (ranges) of POPs in gizzard shad were in the following order: DDTs (3900-16,000), chlordanes (3400-14,000), cyclodienes (340-1300), HCB (110-480), and HCHs (140-360) on nanogram per gram lipid basis.
authors.affiliation: Shimadzu Techno-Research Inc., Nishinokyo-Shimoaicho, Nakagyo-ku, Kyoto, Japan. kskumar@shimadzu-techno.co.jp
authors.forename: Kurunthachalam Senthil
authors.initials: KS
authors.lastname: Kumar
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takemori
authors.forename: Naomasa
authors.initials: N
authors.lastname: Iseki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Masunaga
authors.forename: Takumi
authors.initials: T
authors.lastname: Takasuga
mesh_terms: D000818:Animals; D001717:Birds / Q000378:metabolism*; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism*; D020387:Food Chain; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D007564:Japan; D008099:Liver / Q000737:chemistry; D010063:Ovum / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000592:standards; D018570:Risk Assessment
chemical_list: D006843:Hydrocarbons, Chlorinated; D010573:Pesticide Residues