Rapid induction of malignant tumor in Sprague-Dawley rats by injection of RK3E-ras cells.
Several tumor animal models have been provided as a tool for developing cancer therapy. Here, we developed rapid, easy-to use, and cost-effective new rat animal model for invasion and metastasis of cancer using genetically k-ras-induced rat kidney cells (RK3E-ras). We observed tumor as early as 3 days after injection of RK3E-ras cells in subcutaneous of Sprague-Dawley rats. Tumor size and volume were increased exponentially for 2 weeks. The tail vein injected rats obtained the lethal infiltration in the lung within 2 weeks. This tumor animal model has great potential for studying cancer processes and short-term screening of variable cancer therapy strategy.
Kim
Soo-A
SA
Oral Biology Research Institute, BK 21 Project, Chosun University College of Dentistry, Gwangju 501-759, South Korea.
Kim
Hyun-Woo
HW
Kim
Do-Kyung
DK
Kim
Su-Gwan
SG
Park
Joo-Cheol
JC
Kang
Dong-Wan
DW
Kim
Si-Wouk
SW
Ahn
Sang-Gun
SG
Yoon
Jung-Hoon
JH
D000818:Animals; D002461:Cell Line, Transformed / Q000637:transplantation; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D011905:Genes, ras / Q000502:physiology*; D007279:Injections, Subcutaneous; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009361:Neoplasm Invasiveness; D009374:Neoplasms, Experimental / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley