title: A cellular system to study the role of nitric oxide in cell death, survival, and migration.
abstract: The gaseous messenger nitric oxide (NO) plays a bewildering number of roles in fundamental processes, such as cell locomotion, differentiation, proliferation and death. Its different and often contrasting roles may depend on its concentration and intracellular site of generation. We describe here a simple system with which to investigate the roles of NO generated at physiological levels in HeLa cells by eNOS transfected under an inducible promoter. This system has allowed us to uncover unexpected signalling circuits between NO and ceramide, involved in the response of cells to apoptotic stimuli. At present, we are using these cells as a tool to investigate the role of NO in migration.
authors.affiliation: Department of Pharmaco-Biological Science, University of Catanzaro "Magna Graecia", Campus of Germaneto, 88100 Catanzaro, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Bulotta
authors.forename: Cristiana
authors.initials: C
authors.lastname: Perrotta
authors.forename: Andrea
authors.initials: A
authors.lastname: Cerullo
authors.forename: Clara
authors.initials: C
authors.lastname: De Palma
authors.forename: Emilio
authors.initials: E
authors.lastname: Clementi
authors.forename: Nica
authors.initials: N
authors.lastname: Borgese
mesh_terms: D017209:Apoptosis / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002518:Ceramides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009389:Neovascularization, Pathologic / Q000473:pathology; D009569:Nitric Oxide / Q000502:physiology*; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection
chemical_list: D002518:Ceramides; D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III