title: Quantitative analysis of topoisomerase IIalpha to rapidly evaluate cell proliferation in brain tumors.
abstract: Immunohistochemical cell proliferation analyses have come into wide use for evaluation of tumor malignancy. Topoisomerase IIalpha (topo IIalpha), an essential nuclear enzyme, has been known to have cell cycle coupled expression. We here show the usefulness of quantitative analysis of topo IIalpha mRNA to rapidly evaluate cell proliferation in brain tumors. A protocol to quantify topo IIalpha mRNA was developed with a real-time RT-PCR. It took only 3 h to quantify from a specimen. A total of 28 brain tumors were analyzed, and the level of topo IIalpha mRNA was significantly correlated with its immuno-staining index (p<0.0001, r=0.9077). Furthermore, it sharply detected that topo IIalpha mRNA decreased in growth-inhibited glioma cell. These results support that topo IIalpha mRNA may be a good and rapid indicator to evaluate cell proliferate potential in brain tumors.
authors.affiliation: Department of Neurosurgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8315, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Oda
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Takahisa
authors.initials: T
authors.lastname: Katsuki
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Shirahata
authors.forename: Makoto
authors.initials: M
authors.lastname: Ono
authors.forename: Norikazu
authors.initials: N
authors.lastname: Yamana
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Jun A
authors.initials: JA
authors.lastname: Takahashi
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002675:Child, Preschool; D004250:DNA Topoisomerases, Type II / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D004250:DNA Topoisomerases, Type II