title: Determination and pharmacokinetics of ergometrine maleate in rabbit blood with on line microdialysis sampling and fluorescence detection.
abstract: The study describes a flow injection on-line microdialysis system for in vivo monitoring of ergometrine maleate in rabbit blood with fluorescence detection. A flow-through microdialysis probe was used for intravenous sampling by pumping of the blood from the tested rabbit through the flow-through microdialysis probe located outside the living system at a flow rate of 15 microl min-1. The perfusion rate is 5 microl min-1. The ergometrine maleate in the dialysate was detected on-line with a flow injection fluorescence system after the ergometrine maleate administration (0.2 mg kg-1, i.v.). The dialysate sample volume was about 15 microl. The system was linearly related to the concentration of ergometrine maleate in the range 1-140 ng ml-1 (r=0.9989) with a detection limit 0.3 ng ml-1 (3sigma). The pharmacokinetic parameters of ergometrine maleate were calculated utilizing the pharmacokinetic software 'NDST-21' by a one-compartmental open model.
authors.affiliation: Institute of Analytical Science, Department of Chemistry, Southwest Normal University, Beibei, Chongqing 400715, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Gong
authors.forename: Yufei
authors.initials: Y
authors.lastname: Hu
authors.forename: Deyong
authors.initials: D
authors.lastname: He
mesh_terms: D000818:Animals; D004874:Ergonovine / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D017551:Microdialysis; D010477:Perfusion; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004874:Ergonovine