Construction of a multifunctional envelope-type nano device by a SUV*-fusion method.
A novel assembly method "SUV*-fusion method" was developed for the construction of a small and homogenous multifunctional envelope-type nano device (MEND) by utilizing a detergent-rich small unilamellar vesicle (SUV*). The method consists of three steps: (1) DNA condensation with a polycation, (2) electrostatic interaction of the SUV* with the DNA/polycation complex (DPC) and (3) lipid coating of DPC by SUV* fusion via removal of the detergent. We confirmed the construction of the MEND by sucrose density gradient centrifugation, and isolated the MEND only from the boundary between 25% and 40% sucrose. The isolated MEND had a small diameter (155 nm), was negatively charged (-24 mV), and encapsulated 30% of the total DNA. The MEND was formed by only SUV*, not by a lipid/detergent micelle. This confirms that a small and homogenous MEND can be constructed by the SUV*-fusion method. Furthermore, we confirmed that a transferrin-modified MEND could deliver a gene into a cell through receptor-mediated endocytosis. Consequently, we report on the successful construction of a small and homogenous MEND by a novel SUV*-fusion method.
Sasaki
Kentaro
K
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo 060-0812, Japan.
Kogure
Kentaro
K
Chaki
Sinji
S
Kihira
Yoshitaka
Y
Ueno
Masaharu
M
Harashima
Hideyoshi
H
D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D049329:Nanostructures / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000008:administration & dosage / Q000138:chemical synthesis / Q000235:genetics; D011073:Polyamines / Q000008:administration & dosage / Q000138:chemical synthesis; D000071228:Polyelectrolytes
D004247:DNA; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011073:Polyamines; D000071228:Polyelectrolytes