Dynamics and function of Langerhans cells in vivo: dermal dendritic cells colonize lymph node areas distinct from slower migrating Langerhans cells.
Langerhans cells (LCs) are prominent dendritic cells (DCs) in epithelia, but their role in immunity is poorly defined. To track and discriminate LCs from dermal DCs in vivo, we developed knockin mice expressing enhanced green fluorescent protein (EGFP) under the control of the langerin (CD207) gene. By using vital imaging, we showed that most EGFP(+) LCs were sessile under steady-state conditions, whereas skin inflammation induced LC motility and emigration to lymph nodes (LNs). After skin immunization, dermal DCs arrived in LNs first and colonized areas distinct from slower migrating LCs. LCs reaching LNs under steady-state or inflammatory conditions expressed similar levels of costimulatory molecules. Langerin and EGFP were also expressed on thymic DCs and on blood-derived, CD8alpha(+) DCs from all secondary lymphoid organs. By using a similar knockin strategy involving a diphtheria toxin receptor (DTR) fused to EGFP, we demonstrated that LCs were dispensable for triggering hapten-specific T cell effectors through skin immunization.
Kissenpfennig
Adrien
A
Centre d'Immunologie de Marseille-Luminy, INSERM-CNRS-Université de la Méditerranée, Parc Scientifique et Technologique de Luminy, Case 906, 13288 Marseille Cedex 09, France.
Henri
Sandrine
S
Dubois
Bertrand
B
Laplace-Builhé
Corinne
C
Perrin
Pierre
P
Romani
Nikolaus
N
Tripp
Christoph H
CH
Douillard
Patrice
P
Leserman
Lee
L
Kaiserlian
Dominique
D
Saeland
Sem
S
Davoust
Jean
J
Malissen
Bernard
B
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D003877:Dermatitis, Contact / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000235:genetics; D066257:Heparin-binding EGF-like Growth Factor; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D037241:Mannose-Binding Lectins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011956:Receptors, Cell Surface / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000293:injuries; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000954:Antigens, Surface; C498497:Cd207 protein, mouse; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins