title: Human netrin-G1 isoforms show evidence of differential expression.
abstract: The recently identified netrins-G1 and -G2 form a distinct subgroup within the UNC-6/netrin gene family of axon guidance molecules. In this study, we determined the size and structure of the exon/intron layout of the human netrin-G1 (NTNG1) and -G2 (NTNG2) genes. Northern analysis of both genes showed limited nonneuronal but wide brain expression, particularly for NTNG2. Reverse transcriptase PCR detected nine alternatively spliced isoforms including four novel variants of NTNG1 from adult brain. A semiquantitative assay established that major expression was restricted to isoforms G1c, G1d, G1a, and G1e in the brain and to G1c in the kidney. There is also evidence of developmental regulation of these isoforms between fetal and adult brain. In conclusion, NTNG1 may use alternative splicing to diversify its function in a developmentally and tissue-specific manner.
authors.affiliation: Laboratory for Molecular Psychiatry, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Joanne M A
authors.initials: JM
authors.lastname: Meerabux
authors.forename: Hisako
authors.initials: H
authors.lastname: Ohba
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fukasawa
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Suto
authors.forename: Mika
authors.initials: M
authors.lastname: Aoki-Suzuki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakashiba
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nishimura
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Itohara
authors.forename: Takeo
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005091:Exons; D005260:Female; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007438:Introns; D007668:Kidney / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000075385:Netrins; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D058851:GPI-Linked Proteins; D006023:Glycoproteins; C463082:NTNG1 protein, human; C463083:NTNG2 protein, human; D009419:Nerve Tissue Proteins; D000075385:Netrins; D020033:Protein Isoforms