Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Batista
Frederico M
FM
Instituto Nacional de Investigação Agrária e das Pescas (INIAPIIPIMAR), CRIPSul, Av. 5 de Outubro, 8700-305 Olhão, Portugal.
Taris
Nicolas
N
Boudry
Pierre
P
Renault
Tristan
T
D000818:Animals; D017756:Aquaculture / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006564:Herpesviridae / Q000235:genetics*; D007814:Larva / Q000821:virology; D010125:Ostreidae / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004247:DNA