White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods.
OBJECTIVE
White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.


METHODS
As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).


RESULTS
Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.


CONCLUSIONS
The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.
Dijkstra-Tiekstra
M J
MJ
Sanquin Blood Bank North-west Region, 100 AC Amsterdam, the Netherlands.
van der Schoot
C E
CE
Pietersz
R N I
RN
Reesink
H W
HW
D004247:DNA / Q000032:analysis; D005374:Filtration; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods* / Q000592:standards
D019007:P-Selectin; D004247:DNA