title: Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster.
abstract: As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.
authors.affiliation: Department of Biology, School of Education, Waseda University, Shinjuku-ku, Tokyo, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ito
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirota
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Matsunaga
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Sakaé
authors.initials: S
authors.lastname: Kikuyama
mesh_terms: D034841:Abdominal Cavity / Q000502:physiology; D000818:Animals; D001141:Aromatase / Q000096:biosynthesis* / Q000235:genetics; D005088:Exocrine Glands / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012455:Salamandridae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D001141:Aromatase