title: Heterogeneities in EGF receptor density at the cell surface can lead to concave up scatchard plot of EGF binding.
abstract: The mechanism responsible for the concave up nature of the Scatchard plot of epidermal growth factor (EGF) binding on EGF receptor (EGFR) has been a controversial issue for more than a decade. Past efforts to mechanistically simulate the concave up nature of the Scatchard plot of EGF binding have shown that negative cooperativity in EGF binding on an EGFR dimer or inclusion of some external site or binding event can describe this behavior. However, herein we show that heterogeneity in the density of EGFR due to localization in certain regions of the plasma membrane, which has been experimentally reported, can also lead to concave up shape of the Scatchard plot of the EGF binding on EGFR.
authors.affiliation: Department of Chemical Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716, USA.
authors.forename: Kapil
authors.initials: K
authors.lastname: Mayawala
authors.forename: Dionisios G
authors.initials: DG
authors.lastname: Vlachos
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Edwards
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D019281:Dimerization; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D018448:Models, Immunological; D013816:Thermodynamics
chemical_list: D008024:Ligands; D004815:Epidermal Growth Factor; D066246:ErbB Receptors