[Effect of nucleolin down-regulation on the proliferation and apoptosis in C2C12 cells].
OBJECTIVE
To clarify the effect of nucleolin on the proliferation and apoptosis in C2C12 cells.


METHODS
After inhibiting the expression of nucleolin using antisense oligonucleotides, the cellular proliferation was determined by MTT, and the apoptosis was detected by flow cytometry (FCM) assays and DNA ladder assays.


RESULTS
After being transfected with antisense oligonucleotides for 24 hours, Western blotting showed that the expression of nucleolin was repressed significantly. In cells treated with antisense oligonucleotides, the cellular proliferation was obviously inhibited; the apoptotic cell increased significantly; and the "DNA ladder" was clearly observed. But the sense and random oligonucleotides had no effect on the cellular proliferation and apoptosis.


CONCLUSIONS
The down-regulation of nucleolin can inhibit the cellular proliferation and initiate the apoptosis in C2C12cells.
Wang
Kang-kai
KK
Department of Pathophysiology , Xiangya School of Medicine, Central South University, Changsha 410078, China.
Jiang
Lei
L
E
Shun-mei
SM
Liu
Ke
K
Zhang
Ling-li
LL
Liu
Mei-dong
MD
Xiao
Xian-zhong
XZ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D051379:Mice; D032446:Myoblasts / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology*; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D000097796:Nucleolin
D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin