title: [Tensional and morphologic analysis of the colonic anastomosis on 10% acetic acid induced colitis, in Wistar rats, treated with 10% aroeira-do-sertão (Myracrodruon urundeuva fr. all.) aqueous extract].
abstract: OBJECTIVE
  Verify, from a morphologic and tensional view point, the effect of the 10% aqueous extract of aroeira-do-sertão on the colonic anastomosis, during the occurrence of 10% acetic acid induced colitis, in Wistar rats.
  
  
  METHODS
  There were used 48 Wistar rats, distributed in two groups. All animals were subjected to induction of colitis by aqueous solution of 10% acetic acid. Twenty-four hours later, the animals were subjected to laparotomy, transverse total colotomy and end-to-end anastomosis with 5-0 polypropylene. Group A (vehicle), animals treated with carboxymethylcellulose based vehicle. Group B (aroeira), animals treated with 10% aroeira aqueous extract. Both treatments were by means of enema. Groups A and B were distributed in subgroups A3, A7, A14 and A21; B3, B7, B 14 and B21, according to the foreseen euthanasia date (days 3, 7, 14 and 21). On these dates, the animals were relaparotomized, the colonic segment containing the anastomosis was ressected subjected to the pressure test and then to the, histological analysis. For the momphologic study, the slides were dyed with hematoxilin-eosine and the healing status was evaluated based on a score chart that ranked from 0 to 16. The higher the score, the better the healing.
  
  
  RESULTS
  On the evaluation of the morphologic study, which quantifies the evolution and degree of healing, as a final result of the healing process, the group aroeira was superior to the group vehicle (p<0.05) on the twenty-first day, showing higher velocity on tissue repair that occurs by regeneration and not by fibrosis. With respect to the tension test, there was a statistically significant difference on day 3, with predominance of the group aroeira over the group vehicle (p<0.05).
  
  
  CONCLUSIONS
  The 10% aroeira-do-sertão aqueous extract, has healing activity on the colonic anstomosis during the occurrence of 10% acetic acid induced colitis. It improves the resistance to tension on the colonic anastomosis zone on the 3rd day.
authors.affiliation: Departamento de Cirurgia da Universidade Federal do Ceará. arsmc@uol.com.br
authors.forename: Antonio Rubens Soares Martins
authors.initials: AR
authors.lastname: Cavalcante
authors.forename: Lusmar Veras
authors.initials: LV
authors.lastname: Rodrigues
authors.forename: Dalgimar Beserra
authors.initials: DB
authors.lastname: de Menezes
authors.forename: Maria do Perpétuo Socorro Saldanha
authors.initials: Mdo P
authors.lastname: da Cunha
authors.forename: Annya Costa Araújo de Macedo
authors.initials: AC
authors.lastname: Goes
mesh_terms: D019342:Acetic Acid; D027926:Anacardiaceae; D000714:Anastomosis, Surgical; D000818:Animals; D001696:Biomechanical Phenomena; D003092:Colitis / Q000503:physiopathology / Q000601:surgery; D003106:Colon / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D019342:Acetic Acid