title: Rotenone induces aggregation of gamma-tubulin protein and subsequent disorganization of the centrosome: relevance to formation of inclusion bodies and neurodegeneration.
abstract: Neurodegenerative disorders are characterized by progressive loss of specific neurons in the central nervous system. Although they have different etiologies and clinical manifestations, most of them share similar histopathologic characteristics such as the presence of inclusion bodies in both neurons and glial cells, which represent intracellular aggregation of misfolded or aberrant proteins. In Parkinson's disease, formation of inclusion bodies has been associated with the aggresome-related process and consequently with the centrosome. However, the significance of the centrosome in the neurodegenerative process remains obscure. In the present study, the morphological and functional changes in the centrosome induced by rotenone, a common insecticide used to produce experimental Parkinsonism, were examined both in vitro and in vivo. Aggregation of gamma-tubulin protein, which is a component of the centrosome matrix and recently identified in Lewy bodies of Parkinson's disease, was observed in primary cultures of mesencephalic cells treated with rotenone. Rotenone-treated neurons and astrocytes showed enlarged and multiple centrosomes. These centrosomes also displayed multiple aggregates of alpha-synuclein protein. Neurons with disorganized centrosomes exhibited neurite retraction and microtubule destabilization, and astrocytes showed disturbances of mitotic spindles. The Golgi apparatus, which is closely related to the centrosome, was dispersed in both rotenone-treated neuronal cells and the substantia nigra of rotenone-treated rats. Our findings suggested that recruitment of abnormal proteins in the centrosome contributed to the formation of inclusion bodies, and that rotenone markedly affected the structure and function of the centrosome with consequent induction of cytoskeleton disturbances, disassembly of the Golgi apparatus and collapse of neuronal cells.
authors.affiliation: Department of Brain Science, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikatacho, Okayama 700-8558, Japan.
authors.forename: F J
authors.initials: FJ
authors.lastname: Diaz-Corrales
authors.forename: M
authors.initials: M
authors.lastname: Asanuma
authors.forename: I
authors.initials: I
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Miyoshi
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D018385:Centrosome / Q000187:drug effects / Q000648:ultrastructure*; D004298:Dopamine / Q000502:physiology; D005260:Female; D006056:Golgi Apparatus / Q000187:drug effects / Q000648:ultrastructure; D018476:Hypokinesia / Q000139:chemically induced; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012402:Rotenone / Q000494:pharmacology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure; D051843:Synucleins; D014404:Tubulin / Q000737:chemistry / Q000187:drug effects*; D014446:Tyrosine 3-Monooxygenase / Q000502:physiology; D014475:Uncoupling Agents / Q000494:pharmacology*; D051844:alpha-Synuclein
chemical_list: D009419:Nerve Tissue Proteins; C497606:Snca protein, rat; D051843:Synucleins; D014404:Tubulin; D014475:Uncoupling Agents; D051844:alpha-Synuclein; D012402:Rotenone; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine