Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo.
A solid tumor is an organ composed of cancer and host cells embedded in an extracellular matrix and nourished by blood vessels. A prerequisite to understanding tumor pathophysiology is the ability to distinguish and monitor each component in dynamic studies. Standard fluorophores hamper simultaneous intravital imaging of these components. Here, we used multiphoton microscopy techniques and transgenic mice that expressed green fluorescent protein, and combined them with the use of quantum dot preparations. We show that these fluorescent semiconductor nanocrystals can be customized to concurrently image and differentiate tumor vessels from both the perivascular cells and the matrix. Moreover, we used them to measure the ability of particles of different sizes to access the tumor. Finally, we successfully monitored the recruitment of quantum dot-labeled bone marrow-derived precursor cells to the tumor vasculature. These examples show the versatility of quantum dots for studying tumor pathophysiology and creating avenues for treatment.
Stroh
Mark
M
E.L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Boston, MA 02114, USA.
Zimmer
John P
JP
Duda
Dan G
DG
Levchenko
Tatyana S
TS
Cohen
Kenneth S
KS
Brown
Edward B
EB
Scadden
David T
DT
Torchilin
Vladimir P
VP
Bawendi
Moungi G
MG
Fukumura
Dai
D
Jain
Rakesh K
RK
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001854:Bone Marrow Cells / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D042783:Endothelial Cells / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D045663:Quantum Dots
D000970:Antineoplastic Agents