title: Exposure to the organophosphorus pesticide chlorpyrifos inhibits acetylcholinesterase activity and affects muscular integrity in Xenopus laevis larvae.
abstract: The effect of organophosphate pesticide chlorpyrifos (CPF) on acetylcholinesterase (AChE) activity and on skeletal muscle development in Xenopus laevis larvae was studied. To achieve our purpose embryos were exposed to 100, 250 and 3000 microg/l CPF concentrations from late blastula stage (8 h postfertilization, p.f.) to stage 47 (120 h p.f.) and the appearance of AChE activity was monitored every 24 h. Compared with control, CPF treated larvae showed a dose dependent AChE inhibition during the early stages (beginning from 24 h until 120 h p.f.) that are crucial for neuromuscular development. The amount of AChE activity that can still be measured in treated larvae at stage 47 relative to that of the control, ranged from 28% in CPFs 100 microg/l to 4% in CPFs 3000 microg/l. These low AChE activities were associated with muscular damages such as reduced myotome size and hypertrophies coupled with extensive vacuolated regions in myocytes. The occurrence of this tissue-specific injury was related to CPF concentrations and was most pronounced in CPFs 3000 microg/l which revealed a very severe AChE inhibition during the exposure. Since AChE is the major neurotransmitter of the neuromuscular system, this initial descriptive study will be an useful starting-point to ongoing and future subcellular/molecular studies that correlate the morphological damage with changes in AChE activity.
authors.affiliation: Dipartimento di Scienze dell'Ambiente e del Territorio, Università degli Studi di Milano Bicocca, Piazza della Scienza 1, I-20126 Milano, Italy. anita.colombo@unimib.it
authors.forename: Anita
authors.initials: A
authors.lastname: Colombo
authors.forename: Federica
authors.initials: F
authors.lastname: Orsi
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bonfanti
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000473:pathology; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004390:Chlorpyrifos / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D009132:Muscles / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D013997:Time Factors; D014982:Xenopus laevis
chemical_list: D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; D010575:Pesticides; D000110:Acetylcholinesterase; D004390:Chlorpyrifos