title: Transfection of mouse macrophage metalloelastase gene into murine CT-26 colon cancer cells suppresses orthotopic tumor growth, angiogenesis and vascular endothelial growth factor expression.
abstract: A cDNA fragment coding for domains I and II of mouse macrophage metalloelastase (MME) was transfected into murine CT-26 colon cancer cells that are MME deficient. An orthotopic implantation model was established by using MME-transfected cells. In MME-transfected primary tumors, it demonstrated that tumor growth and microvessel formation were significantly inhibited compared with the controls. The expression of vascular endothelial growth factor (VEGF) mRNA and protein was significantly lower in MME-transfected group compared with those in the controls. Our data show that both MME and VEGF gene expression is highly associated with the vascularity of tumors, which may depend on a balance between MME and VEGF expression.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital, Anhui Medical University, Hefei 23022, Anhui Province, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Shi
authors.forename: Jian Ming
authors.initials: JM
authors.lastname: Xu
authors.forename: Nai Zhong
authors.initials: NZ
authors.lastname: Hu
authors.forename: Xue Long
authors.initials: XL
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Mei
authors.forename: Yu Lin
authors.initials: YL
authors.lastname: Song
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000098:blood supply / Q000473:pathology / Q000628:therapy*; D015316:Genetic Therapy; D005822:Genetic Vectors; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053508:Matrix Metalloproteinase 12; D008666:Metalloendopeptidases / Q000235:genetics* / Q000502:physiology; D051379:Mice; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
chemical_list: D042461:Vascular Endothelial Growth Factor A; D008666:Metalloendopeptidases; D053508:Matrix Metalloproteinase 12