BKV infection and hemorrhagic cystitis after allogeneic bone marrow transplant.
Hemorrhagic cystitis (HC) is a well-known complication after allogeneic bone marrow transplant (BMT) and can be related to adenovirus or human polyomavirus BK (BKV) infections. In this study a group of 20 patients after allogeneic BMT has been examined. BMT urine samples were analysed for the presence of Adenovirus and BKV DNAby means of polymerase chain reaction (PCR). 5/20 BMT patients developed HC after BMT. The presence of BKV DNA in urine samples was evident in 3/15 patients without HC and in 5/5 patients with HC. In 2/5 HC-patients the BKV DNA was not found after therapy with Cidofovir and Ribavirin. The search for adenovirus DNA in all samples was negative. The analysis of BKV non-coding control region (NCCR) isolated from urine samples revealed a structure very similar to the archetype in all samples. The RFLP (Restriction Fragment Length Polymorphism assay) showed the presence of BKV subtypes I and IV, with the prevalence of subtype I (4/5). This study supports the hypothesis that HC is mainly related to BKV rather than to adenovirus infection in BMT patients. Moreover, since BKV subtype I was predominant, it is reasonable to hypothesize that a specific BKV subtype could be associated with the development of HC.
Fioriti
D
D
Department of Public Health Sciences, University La Sapienza, Rome, Italy.
Degener
A M
AM
Mischitelli
M
M
Videtta
M
M
Arancio
A
A
Sica
S
S
Sora
F
F
Pietropaolo
V
V
D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000652:urine / Q000821:virology; D000328:Adult; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D003556:Cystitis / Q000652:urine / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis* / Q000652:urine; D005260:Female; D006470:Hemorrhage / Q000652:urine / Q000821:virology*; D006801:Humans; D019901:Locus Control Region / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D027601:Polyomavirus Infections / Q000652:urine / Q000821:virology*; D016415:Sequence Alignment; D014184:Transplantation, Homologous; D014556:Urine / Q000821:virology
D004279:DNA, Viral