delta-Opioid agonist induced regulation of E2F1 DNA binding activity in NG108-15 cells.
Activation of opioid receptors have been implicated in the modulation of cell proliferation and the E2F family of transcription factors may play a role in opioid inhibition of DNA synthesis. Gel shift assays and Western blotting of nuclear extracts from NG108-15 cells revealed increased E2F1 DNA binding activity and higher levels of E2F1 following activation of delta-opioid receptors. It is suggested that DADLE-induced regulation of E2F DNA binding activity involves ERKs.
Tencheva
Zvetanka S
ZS
Institute of Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Blok 23, 1113 Sofia, Bulgaria. Tencheva@mail.bol.bg
Praskova
Maria D
MD
Velichkova
Adriana A
AA
Mitev
Vanio I
VI
D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods; D007093:Imidazoles / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D011485:Protein Binding / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D017465:Receptors, Opioid, delta / Q000187:drug effects*; D013936:Thymidine / Q000493:pharmacokinetics; D014157:Transcription Factors / Q000378:metabolism*; D014316:Tritium / Q000493:pharmacokinetics
D000701:Analgesics, Opioid; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D004791:Enzyme Inhibitors; D005419:Flavonoids; D007093:Imidazoles; D011725:Pyridines; D017465:Receptors, Opioid, delta; D014157:Transcription Factors; D014316:Tritium; D016308:Enkephalin, Leucine-2-Alanine; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D013936:Thymidine