Limiting iron availability confers neuroprotection from chronic mild carbon monoxide exposure in the developing auditory system of the rat.
Iron deficiency and chronic mild carbon monoxide (CO) exposure are nutritional and environmental problems that can be experienced simultaneously. We examined the effects of chronic mild CO exposure and iron availability on auditory development in the rat. We propose that chronic mild CO exposure creates an oxidative stress condition that impairs the spiral ganglion neurons. The CO-exposed rat pups had decreased neurofilament proteins and increased copper, zinc-superoxide dismutase (SOD1) in the spiral ganglion neurons. We conclude that the increased amount of SOD1 causes an increase in hydrogen peroxide production that allows the Fenton reaction to occur. This reaction uses both iron and hydrogen peroxide to generate hydroxyl radicals and leads to the development of oxidative stress that impairs neuronal integrity. However, rat pups with decreased iron and CO exposure (ARIDCO) exhibited in their cochlea an up-regulation of transferrin, whereas their expression of neurofilament proteins and SOD1 were similar to control. Consequently, reduced iron availability and the normal expression of SOD1 do not promote oxidative stress in the cochlea. By using basal c-Fos expression as a marker for cellular activation we found a significant reduction in c-Fos expression in the central nucleus of the inferior colliculus in iron-adequate rat pups exposed to CO. By contrast, rather than being reduced, c-Fos expression in the ARIDCO group is the same as for controls. We conclude that the cochlea of rat pups with normal iron availability is selectively affected by mild CO exposure, causing a chronic oxidative stress, whereas limiting iron availability ameliorates the effect caused by mild CO exposure by averting conditions that facilitate oxidative stress.
Webber
Douglas S
DS
Mental Retardation Research Center, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Lopez
Ivan
I
Korsak
Rose A
RA
Hirota
Sean
S
Acuna
Dora
D
Edmond
John
J
D000818:Animals; D001306:Auditory Pathways / Q000254:growth & development / Q000378:metabolism; D002248:Carbon Monoxide / Q000633:toxicity; D002249:Carbon Monoxide Poisoning / Q000378:metabolism*; D003051:Cochlea / Q000254:growth & development* / Q000378:metabolism*; D006400:Hematocrit; D007245:Inferior Colliculi / Q000254:growth & development* / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D041941:Milk Substitutes; D016900:Neurofilament Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D012720:Severity of Illness Index; D013136:Spiral Ganglion / Q000254:growth & development / Q000378:metabolism
D016900:Neurofilament Proteins; D002248:Carbon Monoxide; D007501:Iron