title: Styrene and ethylene glycol have a synergetic effect on lipid peroxidation that is better protected than repaired by CoQ10.
abstract: Previous study of a group of 22 workers occupationally exposed to styrene, ethylene glycol and their mixture at a paint and lacquer industry indicated significantly elevated concentration of malondialdehyde with 4-hydroxynonenal (MDA+4-HNE) in the blood plasma, successfully decreased with coenzyme Q10 (CoQ10) supplementation. The aim of present study is to evaluate whether the exposure to styrene or/and ethylene glycol could be responsible for the increase in MDA level. The mechanism of a single solvent action and the mixture was examined, specially whether it is connected with hydroxyl radical (*OH) generation. It was also investigated whether coenzyme Q10 could be considered as a protective (given before the solvents) or repairing (given after the solvents) agent in oxidative stress caused by the solvents. The results indicate that ethylene glycol nor styrene increase MDA and *OH, but as a mixture give synergetic interaction, elevating MDA and *OH concentration to a statistically significant extent. Coenzyme Q10 at a dose of 3.0 microg/ml only protects, but does not repair increased lipid peroxidation caused by ethylene glycol with styrene. In order to obtain both a protective and repairing effect, a concentration of 12.0 microg/ml CoQ is needed.
authors.affiliation: Department of Toxicology, Wroclaw Medical University, 54-117 Wroclaw, Traugutta 57/59, Poland. adltox@ak.am.wroc.pl
authors.forename: A
authors.initials: A
authors.lastname: Długosz
authors.forename: E
authors.initials: E
authors.lastname: Sawicka
authors.forename: Z
authors.initials: Z
authors.lastname: Marchewka
mesh_terms: D003067:Coenzymes; D004357:Drug Synergism; D019855:Ethylene Glycol / Q000633:toxicity*; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D020058:Styrene / Q000633:toxicity*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D003067:Coenzymes; D005609:Free Radicals; D014451:Ubiquinone; D020058:Styrene; D008315:Malondialdehyde; C024989:coenzyme Q10; D019855:Ethylene Glycol