title: Serological diagnosis of Trichinella spiralis in experimentally infected mice.
abstract: ELISA and IFA tests were used to detect IgG levels in sera of experimentally infected mice with Trichinella spiralis, during a period of 12 weeks post infection. A crude saline extract of muscle larvae was used for application of ELISA technique, while the intact T. spiralis muscle larvae were used as an antigen for IFAT. In both tests, T. spiralis IgG antibodies could be detected one week post infection in sera of all infected mice. ELISA test showed a sensitivity of 100% during the whole period of the experiment, while its specificity was 93.3%. Meanwhile, IFA test revealed a sensitivity of 100% and a specificity of 85%.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Atef M
authors.initials: AM
authors.lastname: El-Shazly
authors.forename: Salah A
authors.initials: SA
authors.lastname: Abu-Elwafa
authors.forename: Hala A
authors.initials: HA
authors.lastname: El-Nahas
authors.forename: Aida A
authors.initials: AA
authors.lastname: Abdel-Mageed
authors.forename: Mohamed
authors.initials: M
authors.lastname: Soliman
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: El-Kadi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G