title: High performance liquid chromatographic methods for the determination of aripiprazole with ultraviolet detection in rat plasma and brain: application to the pharmacokinetic study.
abstract: High performance liquid chromatographic (HPLC) methods were validated for the determination of aripiprazole (OPC-14597, Abilify) in rat plasma and brain. Separation was by Nova-pak phenyl column; flow rate, 1.0 ml/min; mobile phase, acetonitrile-methanol-20 mM sodium sulfate-acetic acid (27:25:48:1, v/v/v/v); UV detection at 254 nm. Reproducibility in plasma and brain showed excellent precision (within 7.8 and 10.6%) and accuracy (96.0-102.4% and 99.0-108.7%) with calibration curve ranges 10.0-2000 ng/ml and 30.0-6000 ng/g, respectively. Validated HPLC methods were successfully applied to pharmacokinetic study of aripiprazole in rats, demonstrating brain concentrations after oral administration five times higher than plasma concentrations.
authors.affiliation: Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan. y_shimokawa@research.otsuka.co.jp
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Shimokawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Eiji
authors.initials: E
authors.lastname: Kashiyama
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Koga
authors.forename: Gohachiro
authors.initials: G
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D000068180:Aripiprazole; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005615:Freezing; D008297:Male; D010879:Piperazines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D014150:Antipsychotic Agents; D010879:Piperazines; D015363:Quinolones; D000068180:Aripiprazole