title: HPLC based activity profiling for 5-lipoxygenase inhibitory activity in Isatis tinctoria leaf extracts.
abstract: In the pursuit of the anti-inflammatory constituents in lipophilic woad extracts, the 5-lipoxygenase (5-LOX) inhibitory activity was investigated by HPLC-based activity profiling. In a low-resolution profiling, two time windows with peaks of activity were found. The first coincided with tryptanthrin, a known dual inhibitor of cyclooxygenase-2 (COX-2) and 5-LOX, whereas the major inhibitory fraction was towards the end of the HPLC run. The active fractions were profiled in a peak-resolved manner, and the compounds analyzed by LC-MS, GC and TLC. The activity in the lipophilic fractions of the Isatis extract could be linked to an unsaturated fatty acid, alpha-linolenic acid.
authors.affiliation: Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, D-07741 Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Oberthür
authors.forename: R
authors.initials: R
authors.lastname: Jäggi
authors.forename: M
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018922:HL-60 Cells; D006801:Humans; D031219:Isatis / Q000737:chemistry*; D016859:Lipoxygenase Inhibitors; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011799:Quinazolines / Q000737:chemistry / Q000494:pharmacology*; D017962:alpha-Linolenic Acid / Q000737:chemistry
chemical_list: D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D011799:Quinazolines; D017962:alpha-Linolenic Acid; C046243:tryptanthrine