title: Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G.
abstract: Conjugation of immunoglobulin G (IgG) from rabbit serum with colloidal gold for sol agglutination assay was conducted. Effects of various buffer systems on conjugation and stability of sols were studied. Colloidal gold assay for detection of human myoglobin and C-reactive protein was developed. It was shown that rabbit IgG had a poor stabilizing effect on colloidal gold sol and therefore that it cannot be effectively used in such assays. In contrast, hen egg immunoglobulin Y (IgY) had excellent stabilizing properties used in various buffer systems and in a wide range of concentrations. IgY was considered as a useful alternative for rabbit antibodies in gold sol agglutination assay.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences, Yerean, Republic of Armenia. VGasparyan@excite.com
authors.forename: Vardan K
authors.initials: VK
authors.lastname: Gasparyan
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis; D002645:Chickens; D017979:Gold Colloid / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D007136:Immunoglobulins / Q000737:chemistry* / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D017979:Gold Colloid; D006454:Hemoglobins; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D002097:C-Reactive Protein