[Liquid chromatography frontal analysis of the protein binding of glimepiride].
OBJECTIVE
To study the protein binding of glimepiride.


METHODS
An HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.4 in a 67 mmol x L(-1) isotonic sodium phosphate buffer at 37 degree C. Other conditions included flow rate of 0.2 mL x min(-1), UV detection at wavelength 230 nm and injection volume 900 microL.


RESULTS
Nonlinear regression parameter estimation was used for the association constant measurement of glimepiride to both primary and secondary sites, which were 5.1 (micromol x L(-1)-1 and 1 for K1 and n1, and 0.017 (micromol x L(-1))-1 and 7 for K2 and n2, respectively.


CONCLUSIONS
The method is shown to be suitable for investigation of protein binding of glimepiride.
Zhou
Da-wei
DW
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China. dawei1204@yahoo.com
Wang
Huai-feng
HF
Li
Fa-mei
FM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007004:Hypoglycemic Agents / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013453:Sulfonylurea Compounds / Q000378:metabolism*
D007004:Hypoglycemic Agents; D012709:Serum Albumin; D013453:Sulfonylurea Compounds; C057619:glimepiride