Evaluation of reference genes for studies of gene expression in human adipose tissue.
OBJECTIVE
The aim of this study was to evaluate reference genes for expression studies of human adipose tissue.


METHODS
Using 52 human adipose tissue expression profiles (HU95), 10 putative reference genes with the lowest variation in expression levels were selected for further studies. Expression stability of these 10 novel and 5 previously established reference genes was evaluated by real-time reverse transcriptase-polymerase chain reaction analysis. For this purpose, 44 adipose tissue biopsies from 27 subjects were chosen to include a wide range of parameters such as sex, age, BMI, depot origin, biopsy procedure, and effects of nutrition.


RESULTS
LRP10 was identified as the gene with the least variation in expression levels. The frequently used reference genes RPLP0, 18S rRNA, PPIA, ACTB, and GAPD were ranked as 4, 6, 7, 8, and 10, respectively.


CONCLUSIONS
Our results suggest that LRP10 is a better choice as reference for expression studies of human adipose tissue compared with the most frequently used reference genes.
Gabrielsson
Britt G
BG
Research Center for Endocrinology and Metabolism, Department of Internal Medicine, Gröna Stråket 8, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. britt.gabrielsson@medic.gu.se
Olofsson
Louise E
LE
Sjögren
Anders
A
Jernås
Margareta
M
Elander
Anna
A
Lönn
Malin
M
Rudemo
Mats
M
Carlsson
Lena M S
LM
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000375:Aging; D001706:Biopsy / Q000379:methods; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D026502:LDL-Receptor Related Proteins; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011973:Receptors, LDL / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
D026502:LDL-Receptor Related Proteins; C510813:LRP10 protein, human; D026901:Membrane Transport Proteins; D011973:Receptors, LDL; C102582:SORL1 protein, human