title: Molecular mapping of periodontal tissues using infrared microspectroscopy.
abstract: BACKGROUND: Chronic periodontitis is an inflammatory disease of the supporting structures of the teeth. Infrared microspectroscopy has the potential to simultaneously monitor multiple disease markers, including cellular infiltration and collagen catabolism, and hence differentiate diseased and healthy tissues. Therefore, our aim was to establish an infrared microspectroscopy methodology with which to analyze and interpret molecular maps defining pathogenic processes in periodontal tissues. METHODS: Specific key cellular and connective tissue components were identified by infrared microspectroscopy and using a chemical imaging method. RESULTS: Higher densities of DNA, total protein and lipid were revealed in epithelial tissue, compared to the lower percentage of these components in connective tissue. Collagen-specific tissue mapping by infrared microspectroscopy revealed much higher levels of collagen deposition in the connective tissues compared to that in the epithelium, as would be expected. Thus inflammatory events such as cellular infiltration and collagen deposition and catabolism can be identified by infrared microspectroscopy. CONCLUSION: These results suggest that infrared microspectroscopy may represent a simple, reagent-free, multi-dimensional tool with which to examine periodontal disease etiology using entirely unprocessed tissue sections.
authors.affiliation: Oral Health and Systemic Disease Research Group, University of Louisville School of Dentistry, Louisville, KY, USA. dascot07@louisville.edu.
authors.forename: Allan
authors.initials: A
authors.lastname: Hynes
authors.forename: David A
authors.initials: DA
authors.lastname: Scott
authors.forename: Angela
authors.initials: A
authors.lastname: Man
authors.forename: David L
authors.initials: DL
authors.lastname: Singer
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sowa
authors.forename: Kan-Zhi
authors.initials: KZ
authors.lastname: Liu