Further studies of lipid droplets in the bombykol-producing pheromone gland of Bombyx mori.
Lipid droplets are abundant in the pheromone-producing cells of B. mori at adult eclosion, followed by daily fluctuations in both their size and number. Their dynamics are related to PBAN-stimulated de novo bombykol production. To elucidate associated events, we performed the following: (1) extraction, purification, and partial characterization of lipid droplet-associated proteins found on their surface since their function could possibly be to transport and/or dock putative lipases that are responsible for the lipolysis of triglycerides in them; (2) separation, purification, and initial analysis of lipids carried by lipophorins and lipid transfer particles originating from pupal and adult hemolymph because of their role in the formation and accumulation of lipid droplets.
Fónagy
Adrien
A
Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary. h7191fon@ella.hu
Ohnishi
Atsushi
A
Esumi
Yasuaki
Y
Suzuki
Yoshikatsu
Y
Matsumoto
Shogo
S
D000818:Animals; D012831:Bombyx / Q000378:metabolism; D005088:Exocrine Glands / Q000378:metabolism; D005233:Fatty Alcohols / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D019476:Insect Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D010675:Pheromones / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification
D005233:Fatty Alcohols; D019476:Insect Proteins; D008055:Lipids; D010675:Pheromones; C006787:bombykol