Production and protein kinase C activation of diacylglycerols containing polymethylene-interrupted PUFA.
Sciadonic acid (20:3, delta-5c,11 c,14c) is a polymethylene-interrupted PUFA (PMI-PUFA) that is present in conifer seeds and known to be incorporated into animal cells and to accumulate in membrane PI as a substitute for arachidonate. In this study, we investigated whether PI having sciadonate could serve as source of DAG that could activate protein kinase C (PKC). When Swiss 3T3 cells cultured with sciadonic acid were stimulated with 100 nM of bombesin, 1-stearoyl-2-sciadonoyl-glycerol (G) and 1-stearoyl-2-arachidonoyl-G were produced. The net increments of these two molecular species of DAG reflected the levels of the two molecular species in the PI in the cells. When cells cultured with juniperonic acid (20:4, delta-5c,11c,14c,17c) were stimulated, 1-stearoyl-2-juniperonoyl-G was produced in proportion to the level of this molecular species in PI in the cells. We also examined PKC activation by synthetic DAG using a partially purified PKC fraction from rat brain and found that both 1-stearoyl-2-sciadonoyl-G and 1-stearoyl-2-juniperonoyl-G could activate PKC comparably to 1 -stearoyl-2-arachidonoyl-G. These results indicate that 1-stearoyl-PI having these C20 PMI-PUFA residues can serve as sources of potential signaling molecules.
Morishige
Jun-ichi
J
Department of Applied Biological Science, Fukuyama University, Fukuyama 729-0292, Japan.
Takai
Yoshimichi
Y
Hirano
Kaoru
K
Tanaka
Tamotsu
T
Satouchi
Kiyoshi
K
D000818:Animals; D001095:Arachidonic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001839:Bombesin / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D004075:Diglycerides / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction; D041701:Swiss 3T3 Cells
D001095:Arachidonic Acids; D004075:Diglycerides; D005231:Fatty Acids, Unsaturated; C437903:juniperonic acid; C045533:eicosa-5,11,14-trienoic acid; D011493:Protein Kinase C; D001839:Bombesin