Sphingopyxis baekryungensis sp. nov., an orange-pigmented bacterium isolated from sea water of the Yellow Sea in Korea.
A Gram-negative, motile, slightly halophilic bacterial strain, SW-150(T), was isolated from sea water of the Yellow Sea, Korea, and was characterized by a polyphasic taxonomic approach. Strain SW-150(T) grew optimally at 25-30 degrees C and in the presence of 2 % (w/v) NaCl. The isolate could be distinguished from other Sphingopyxis species in producing an orange pigment. It contained ubiquinone-10 as the predominant respiratory lipoquinone and C(18 : 1)omega7c and C(17 : 1)omega6c as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 63 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SW-150(T) was phylogenetically affiliated to the genus Sphingopyxis of the family Sphingomonadaceae. Similarity values between the 16S rRNA gene sequences of strain SW-150(T) and the type strains of Sphingopyxis species ranged from 91.6 to 94.2 %, making it possible to categorize strain SW-150(T) as a species that is separate from previously described Sphingopyxis species. On the basis of phenotypic properties and phylogenetic distinctiveness, SW-150(T) (=KCTC 12231(T) = DSM 16222(T)) should be classified as the type strain of a novel Sphingopyxis species, for which the name Sphingopyxis baekryungensis sp. nov. is proposed.
Yoon
Jung-Hoon
JH
Laboratory of Microbial Function, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Lee
Choong-Hwan
CH
Laboratory of Microbial Function, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Yeo
Soo-Hwan
SH
Center for Traditional Microorganism Resources, Keimyung University, Shindang-dong, Dalseo-gu, Daegu, Korea.
Oh
Tae-Kwang
TK
Laboratory of Microbial Function, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D006131:Growth Inhibitors / Q000494:pharmacology; D007723:Korea; D008969:Molecular Sequence Data; D009068:Movement; D010619:Phenazines; D010743:Phospholipids / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013107:Sphingolipids / Q000032:analysis / Q000302:isolation & purification; D042301:Sphingomonadaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis / Q000302:isolation & purification; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D006131:Growth Inhibitors; D010619:Phenazines; D010743:Phospholipids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013107:Sphingolipids; D014451:Ubiquinone; D012965:Sodium Chloride; D005840:Gentian Violet