Methylotrophic autotrophy in Beijerinckia mobilis.
Representatives of the genus Beijerinckia are known as heterotrophic, dinitrogen-fixing bacteria which utilize a wide range of multicarbon compounds. Here we show that at least one of the currently known species of this genus, i.e., Beijerinckia mobilis, is also capable of methylotrophic metabolism coupled with the ribulose bisphosphate (RuBP) pathway of C1 assimilation. A complete suite of dehydrogenases commonly involved in the sequential oxidation of methanol via formaldehyde and formate to CO2 was detected in cell extracts of B. mobilis grown on CH3OH. Carbon dioxide produced by oxidation of methanol was further assimilated via the RuBP pathway as evidenced by reasonably high activities of phosphoribulokinase and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). Detection and partial sequence analysis of genes encoding the large subunits of methanol dehydrogenase (mxaF) and form I RubisCO (cbbL) provided genotypic evidence for methylotrophic autotrophy in B. mobilis.
Dedysh
Svetlana N
SN
S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia. s.dedysh@mtu-net.ru
Smirnova
Ksenia V
KV
Khmelenina
Valentina N
VN
Suzina
Natalia E
NE
Liesack
Werner
W
Trotsenko
Yuri A
YA
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D042062:Beijerinckiaceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D017353:Gene Deletion; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism
D002244:Carbon; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D017853:Phosphotransferases (Alcohol Group Acceptor); C022390:phosphoribulokinase; D012273:Ribulose-Bisphosphate Carboxylase; D005947:Glucose; D009584:Nitrogen