Functional analysis of transcriptional repressor Otx3/Dmbx1.
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.
Kimura
Keita
K
Division of Cellular and Molecular Medicine, Kobe University, Graduate School of Medicine, Japan.
Miki
Takashi
T
Shibasaki
Tadao
T
Zhang
Yan
Y
Ogawa
Makoto
M
Saisho
Hiromitsu
H
Okuno
Masaaki
M
Iwanaga
Toshihiko
T
Seino
Susumu
S
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D019281:Dimerization; D018398:Homeodomain Proteins / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology*; D051857:Otx Transcription Factors; D012097:Repressor Proteins / Q000737:chemistry / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
D017931:DNA Primers; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051857:Otx Transcription Factors; C470836:Otx3 protein, mouse; D012097:Repressor Proteins