title: Correlation between the procedure for antifungal susceptibility testing for Candida spp. of the European Committee on Antibiotic Susceptibility Testing (EUCAST) and four commercial techniques.
abstract: The correlation between results obtained with the European Committee on Antibiotic Susceptibility Testing (EUCAST) antifungal susceptibility testing procedure (document 7.1) and four commercial systems was evaluated for a collection of 93 clinical isolates of Candida spp. Overall, agreement between the EUCAST procedure and the Sensititre YeastOne and Etest methods was 75% and 90.4%, respectively. The correlation indices (p < 0.01) between the EUCAST and commercial methods were 0.92 for Sensititre YeastOne, 0.89 for Etest, - 0.90 for Neo-Sensitabs, and 0.95 for Fungitest. Amphotericin B MICs obtained by Sensititre YeastOne were consistently higher than with the EUCAST method and, although very major errors were not observed, 91% of MICs were misclassified. Amphotericin B- and fluconazole-resistant isolates were identified correctly with Sensititre YeastOne, Etest and Fungitest. Neo-Sensitabs identified amphotericin B-resistant isolates, but misclassified > 5% of fluconazole-resistant isolates as susceptible. The commercial methods, particularly Etest and Fungitest, appeared to be suitable alternatives to the EUCAST procedure for antifungal susceptibility testing of clinical isolates of Candida.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. mcuenca-estrella@isciii.es
authors.forename: M
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: A
authors.initials: A
authors.lastname: Gomez-Lopez
authors.forename: E
authors.initials: E
authors.lastname: Mellado
authors.forename: J L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005060:Europe; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000592:standards*; D011367:Professional Staff Committees; D011933:Reagent Kits, Diagnostic / Q000592:standards*
chemical_list: D000935:Antifungal Agents; D011933:Reagent Kits, Diagnostic