A novel A*24 allele, A*2442, was detected through routine bone marrow donor screening.
In this article, we report the identification of a new HLA-A allele found in a DNA sample which was part of the routine bone marrow donor typing performed in our laboratory. This novel allele officially designated as A*2442 was found in a sample from a female Caucasoid donor (Franken, Bavaria, Germany; lab code 142654) and differs from the closest related allele A*2408 by two nucleotide exchanges. In position 81, the A (A*2408) is changed to 81 C in the novel allele A*2442, resulting in an amino acid substitution at codon 27, glutamine (A*2408) is replaced by histidine ((31)Gln-->(31)His). In position 292, the G (A*2408) is changed to C also resulting in an amino acid replacement, the codon 98 asparagine is mutated to histidine ((98)Asn-->(98)His) in the new A*2442 allele. The second allele was determined to be A*0301. Further typing of this sample is B*1501 B*4001.
Witter
K
K
Labor of Immunogenetics, University Munich, 80336 Munich, Germany. kwitter@kk-i.med.uni-muenchen.de
Sala
M
M
Zahn
R
R
McNicholas
A
A
Volgger
A
A
Albert
E D
ED
D000483:Alleles; D001216:Asparagine / Q000737:chemistry; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D005260:Female; D005973:Glutamine / Q000737:chemistry; D015234:HLA-A Antigens / Q000235:genetics*; D059871:HLA-A24 Antigen; D006579:Heterozygote; D006639:Histidine / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014015:Tissue Banks; D014019:Tissue Donors
D017931:DNA Primers; D015234:HLA-A Antigens; C503953:HLA-A*24:42 antigen; D059871:HLA-A24 Antigen; D005973:Glutamine; D006639:Histidine; D001216:Asparagine