title: Mechanism(s) of increased vascular cell adhesion on nanostructured poly(lactic-co-glycolic acid) films.
abstract: Studies have shown that poly(lactic-co-glycolic acid) (PLGA) films with nanometer surface features promote vascular endothelial and smooth muscle cell adhesion. The objective of this in vitro research was to begin to understand the mechanisms behind this observed increase in vascular cell adhesion. Results provided evidence that nanostructured PLGA adsorbed significantly more vitronectin and fibronectin from serum compared to conventional (or those not possessing nanometer surface features) PLGA. When separately preadsorbing both vitronectin and fibronectin, increased vascular smooth muscle and endothelial cell density was observed on nanostructured (compared to conventional) PLGA. Additionally, blocking of cell-binding epitopes of fibronectin and vitronectin significantly decreased vascular cell adhesion on nanostructured (compared to conventional) PLGA. For this reason, results of the present in vitro study demonstrated that cell adhesive proteins adsorbed in different quantities and altered bioactivity on nanostructured compared to conventional PLGA topographies, which (at least in part) may account for the documented increased vascular cell adhesion on nanostructured PLGA. In this manner, this study continues to provide evidence for the promise of nanostructured PLGA in vascular tissue engineering applications.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907-2022, USA.
authors.forename: Derick C
authors.initials: DC
authors.lastname: Miller
authors.forename: Karen M
authors.initials: KM
authors.lastname: Haberstroh
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D020341:Absorbable Implants; D000327:Adsorption; D000818:Animals; D001011:Aorta / Q000166:cytology; D001665:Binding Sites; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D006016:Glycolates / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D036103:Nanotechnology; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D019096:Vitronectin / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D016895:Culture Media, Serum-Free; D000939:Epitopes; D005353:Fibronectins; D006016:Glycolates; D011108:Polymers; D019096:Vitronectin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid