title: [Experimental model for treating pulmonary metastatic melanoma using grape-seed extract, red wine and ethanol].
abstract: BACKGROUND
  Melanoma is one of the neoplasias that most frequently metastasize, especially in the lung, where represents a challenge in oncology since current treatment is ineffective, and mortality is high.
  
  
  METHODS
  Swiss mice (n = 52) were inoculated with 0.5 x 106 B16F10 cell lines and, later, given an oral administration of grape-seed extract, red wine or ethanol. Metastatic nodules on the lung surface were counted and, after processing for microscopy, five sections were selected for image analysis and the invasion index was calculated.
  
  
  RESULTS
  Macroscopic analysis showed that grape-seed extract and red wine reduced the number of metastatic nodules by 26.07 and 20.81%, respectively, compared with a control group treated with ethanol. Microscopically, the reduction in the invasion index was 31.65 for grape-seed extract and 17.57% for red wine.
  
  
  CONCLUSIONS
  Ethanol administration significantly increased pulmonary metastasis while grape-seed extract and red wine led to their reduction.
authors.affiliation: Cátedra de Anatomía Patológica, Facultad de Medicina, Universidad de Murcia, Murcia, Spain. crismart@um.es
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez Conesa
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente Ortega
authors.forename: M Josefa
authors.initials: MJ
authors.lastname: Yáñez Gascón
authors.forename: Juana Maria
authors.initials: JM
authors.lastname: García Reverte
authors.forename: Manuel
authors.initials: M
authors.lastname: Canteras Jordana
authors.forename: Miguel
authors.initials: M
authors.lastname: Alcaraz Baños
mesh_terms: D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D000431:Ethanol / Q000627:therapeutic use*; D005260:Female; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D012639:Seeds; D027843:Vitis; D014920:Wine
chemical_list: D010936:Plant Extracts; D000431:Ethanol