A novel Porphyromonas gingivalis FeoB plays a role in manganese accumulation.
FeoB is an atypical transporter that has been shown to exclusively mediate ferrous ion transport in some bacteria. Unusually the genome of the periodontal pathogen Porphyromonas gingivalis has two genes (feoB1 and feoB2) encoding FeoB homologs, both of which are expressed in bicistronic operons. Kinetic analysis of ferrous ion transport by P. gingivalis W50 revealed the presence of a single, high affinity system with a K(t) of 0.31 microM. FeoB1 was found to be solely responsible for this transport as energized cells of the isogenic FeoB1 mutant (W50FB1) did not transport radiolabeled iron, while the isogenic FeoB2 mutant (W50FB2) transported radiolabeled iron at a rate similar to wild type. This was reflected in the iron content of W50FB1 grown in iron excess conditions which was approximately half that of the wild type and W50FB2. The W50FB1 mutant had increased sensitivity to both oxygen and hydrogen peroxide and was avirulent in an animal model of infection whereas W50FB2 exhibited the same virulence as the wild type. Analysis of manganous ion uptake using inductively coupled plasma-mass spectrometry revealed a greater than 3-fold decrease in intracellular manganese accumulation in W50FB2 which was also unable to grow in manganese-limited media. The protein co-expressed with FeoB2 appears to be a novel FeoA-MntR fusion protein that exhibits homology to a manganese-responsive, DNA-binding metalloregulatory protein. These results indicate that FeoB2 is not involved in iron transport but plays a novel role in manganese transport.
Dashper
Stuart G
SG
Center for Oral Health Science, School of Dental Science, The University of Melbourne, Victoria 3010, Australia.
Butler
Catherine A
CA
Lissel
J Patricia
JP
Paolini
Rita A
RA
Hoffmann
Brigitte
B
Veith
Paul D
PD
O'Brien-Simpson
Neil M
NM
Snelgrove
Sarah L
SL
Tsiros
John T
JT
Reynolds
Eric C
EC
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001692:Biological Transport; D027682:Cation Transport Proteins / Q000235:genetics* / Q000502:physiology*; D003470:Culture Media / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000502:physiology*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007477:Ions / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D008345:Manganese / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011552:Pseudomonas Infections / Q000378:metabolism; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014774:Virulence
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; C470580:FeoB protein, E coli; C512600:FeoB2 protein, Porphyromonas gingivalis; D007477:Ions; D008345:Manganese; D012313:RNA; D004247:DNA; D006861:Hydrogen Peroxide; D007501:Iron; D010100:Oxygen