Detection of Lawsonia intracellularis using immunomagnetic beads and ATP bioluminescence.
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in various animals. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we have developed an immunological method using immunomagnetic separation and ATP bioluminescence. Magnetic beads were coated with an anti-Lawsonia surface antigen (LsaA) antibody in order to capture the L. intracellularis in fecal samples from infected rabbits and the bacteria captured by the immunomagnetic beads were assayed by means of ATP bioluminescence. Our results showed that L. intracelluraris was detected by immunomagnetic separation of bacteria-holding magnetic beads and ATP-based bioluminescence, suggesting that our methods could be useful for the diagnosis of proliferative enteropathy.
Watarai
Masahisa
M
Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Yamato
Yosuke
Y
Murakata
Kayo
K
Kim
Suk
S
Omata
Yoshitaka
Y
Furuoka
Hidefumi
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial; D045824:Desulfovibrionaceae Infections / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology*; D018189:Immunomagnetic Separation / Q000379:methods*; D007211:Indoles; D007410:Intestinal Diseases / Q000175:diagnosis* / Q000382:microbiology; D020639:Lawsonia Bacteria / Q000302:isolation & purification*; D008156:Luciferases / Q000378:metabolism; D011817:Rabbits
D000907:Antibodies, Bacterial; D007211:Indoles; C007293:DAPI; D000255:Adenosine Triphosphate; D008156:Luciferases