title: Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection.
abstract: A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains, occurring in soy. Group A soyasaponins in different degrees of acetylation, as well as group B soyasaponins in both their 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated and non-conjugated forms could be separated and quantified using authentic soyasaponin standards, in one single run. The method was tested by the determination of the soyasaponin content and composition of eight soygerm samples of different origin. Differences in the composition and the degree of acetylation of the group A soyasaponins were observed among these samples. The group B soyasaponins showed much less variability and they were mainly present in their DDMP-conjugated form.
authors.affiliation: Laboratory of Microbial Ecology & Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Decroos
authors.forename: J P
authors.initials: JP
authors.lastname: Vincken
authors.forename: L
authors.initials: L
authors.lastname: Heng
authors.forename: R
authors.initials: R
authors.lastname: Bakker
authors.forename: H
authors.initials: H
authors.lastname: Gruppen
authors.forename: W
authors.initials: W
authors.lastname: Verstraete
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006031:Glycosylation; D008027:Light; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012542:Scattering, Radiation
chemical_list: D012503:Saponins