title: Isethionate as a product from taurine during nitrogen-limited growth of Klebsiella oxytoca TauN1.
abstract: Klebsiella oxytoca TauN1 represents a group of isolates which utilise taurine (2-aminoethanesulfonate) quantitatively as a sole source of combined nitrogen for aerobic growth. During growth, a compound is excreted, which has now been identified as isethionate (2-hydroxyethanesulfonate). An ion-chromatographic separation of isethionate was developed to quantify the putative isethionate, whose identity was confirmed by matrix-assisted, laser-desorption ionisation time-of-flight mass spectrometry. Strain TauN1 utilised taurine (and excreted isethionate) concomitantly with growth. Cell-free extracts contained inducible taurine transaminase, which yielded sulfoacetaldehyde. A soluble, NADP-dependent isethionate dehydrogenase converted sulfoacetaldehyde to isethionate. The enzyme was partially purified and it apparently belonged to the family of short-chain alcohol dehydrogenases.
authors.affiliation: Fachbereich Biologie der Universität Konstanz, 78457 Konstanz, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Styp von Rekowski
authors.forename: Karin
authors.initials: K
authors.lastname: Denger
authors.forename: Alasdair M
authors.initials: AM
authors.lastname: Cook
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D004790:Enzyme Induction; D007513:Isethionic Acid / Q000378:metabolism*; D041121:Klebsiella oxytoca / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013654:Taurine / Q000378:metabolism*; D000637:Transaminases / Q000096:biosynthesis
chemical_list: C003126:phosphonoacetaldehyde; D013654:Taurine; D007513:Isethionic Acid; D000429:Alcohol Oxidoreductases; C505813:isethionate dehydrogenase; D000637:Transaminases; C022714:taurine aminotransferase; D000079:Acetaldehyde; D009584:Nitrogen