Use of sodium transfer tissue biosensor (STTB) for monitoring of marine toxic organism.
A highly sensitive sodium (Na+) transfer tissue biosensor (STTB) was designed using a frog bladder membrane to measure paralytic shellfish poisons (PSP). The STTB consists, of a Na+ electrode covered by the membrane, which was then integrated into a flow-through system for continuous measurements. In the absence of Na+ channel blocker, active transfer of Na+ occurred from inside to outside across the frog membrane. When the STTB was used to measure the Na+ -dependent dissociation of PSP, it was able to detect PSB at a level contained in a single cell. However, 5 fg or higher (100 cells or more) is needed for accurate and reproducible measurements. The toxicity obtained by the STTB was significantly correlated (r = 0.9449) to that determined by the HPLC. Therefore, the simple method of the STTB can be used not only to detect a low level PSP in toxic plankton populations, but also to monitor poisons in shellfish.
Yoo
J S
JS
Research Institute of Marine Science and Technology, Korea Maritime University, Busan 606-791, Korea. jsyoo@hhu.ac.kr
Cheun
B S
BS
Park
I S
IS
Song
Y C
YC
Seo
Y
Y
Kim
N G
NG
Shin
H W
HW
Lee
J H
JH
D000818:Animals; D001001:Anura; D001693:Biological Transport, Active / Q000502:physiology; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D004141:Dinoflagellida / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis*; D008566:Membranes / Q000378:metabolism; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000032:analysis*; D012964:Sodium / Q000378:metabolism; D013779:Tetrodotoxin / Q000032:analysis*; D001743:Urinary Bladder / Q000378:metabolism
D008387:Marine Toxins; D012530:Saxitoxin; D013779:Tetrodotoxin; C013007:gonyautoxins; D012964:Sodium