title: Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells.
abstract: Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5'-flanking region of human MEF gene and identified a TATA-less promoter region at -204/-54 which contains 4 putative binding sites for Sp1, two of which are essential in up-regulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene.
authors.affiliation: Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Koga
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Suico
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Manabu
authors.initials: M
authors.lastname: Taura
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shuto
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Okiyoneda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kai
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D015536:Down-Regulation; D036383:Ephrin-A2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D036383:Ephrin-A2; D016329:Sp1 Transcription Factor; D004247:DNA