Experimental abscess formation caused by human dental plaque.
Human dental plaque consists of a wide variety of microorganisms, some of which are believed to cause systemic infections, including abscesses, at various sites in the body. To confirm this hypothesis experimentally, we examined the abscess-forming ability of native dental plaque in mice, the microbial features of the infectious locus produced by the plaque, and the anti-phagocytic property of microbial isolates. Aliquots of a suspension of supragingival dental plaque containing 6 x 10(6) colony-forming unit of bacteria were injected subcutaneously into the dorsa of mice. Abscess formation was induced in 76 of 85 mice using ten different plaque samples. Thirteen microorganisms were isolated from pus samples aspirated from abscess lesions. The microbial composition of pus, examined in 17 of 76 abscesses, was very simple compared to that of the plaque sample that had induced the abscess. The majority of the isolates belonged to the Streptococcus anginosus group, normally a minor component of plaque samples. S. anginosus was the most frequently detected organism and the most prevalent in seven abscesses, and Streptococcus intermedius and Streptococcus constellatus were predominant in one and three abscess samples, respectively. Each isolate of S. anginosus group produced abscesses in mice, and heat-treated supragingival dental plaque influenced the abscess-forming ability of S. anginosus isolate. These isolates possessed a high antiphagocytic capacity against human polymorphonuclear leukocytes. Our results suggest that human supragingival dental plaque itself is a source of the infectious pathogens that cause abscess formation.
Okayama
Hidehito
H
Department of Preventive Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Japan.
Nagata
Emi
E
Ito
Hiro-O
HO
Oho
Takahiko
T
Inoue
Masakazu
M
D000038:Abscess / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D000328:Adult; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity*; D003773:Dental Plaque / Q000150:complications / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D034366:Streptococcus anginosus / Q000302:isolation & purification / Q000472:pathogenicity; D034365:Streptococcus constellatus / Q000302:isolation & purification / Q000472:pathogenicity; D034367:Streptococcus intermedius / Q000302:isolation & purification / Q000472:pathogenicity