[Microsatellite instability and loss of heterozygosity of fragile histidine triad gene in laryngeal squamous cell carcinoma].
OBJECTIVE
To investigate the loss of heterozygosity (LOH) and microsatellite instability (MSI) of fragile histidine triad (FHIT) gene in laryngeal squamous cell carcinoma (LSCC).


METHODS
Using polymerase chain reaction-simple sequence length polymorphism (SSLP) -silver staining technique, 41 samples of LSCC were detected for LOH and MSI with microsatellite sites D3S1234 and D3S1300.


RESULTS
LOH was found in 44.4% (16/36) and 36.4% (12/33) of informative cases at D3S1234 and D3S1300 respectively, while MSI at the two loci was found in 19.4% (7/36) and 24.2% (8/33) of informative cases respectively. In 41 cases of LSCC, 38 cases were informative at either locus. 52.6% (20/38) of cases had LOH while 28.9% (11/38) had MSI. The rate of LOH was related to the tumor TNM stage, pathological grade, lymph node metastasis and tumor relapse (P < 0.05). The rate of MSI was related to the tumor lymph node metastasis (P < 0.05 ).


CONCLUSIONS
The frequencies of LOH and MSI in the two microsatellite sites, D3S1234 and D3S1300, of FHIT gene might play a role in carcinogenesis and development of LSCC and might provide some new approaches for early gene detection for LSCC.
Yin
De-Tao
DT
Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. detaoyin@zzu.edu.cn
Dong
Ming-Min
MM
D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase