title: [Preparative isolation and purification of cinnamoyl-C-glycoside chromone from aloe vera by high-speed countercurrent chromatography].
abstract: Aloe chromone is a group of anti-inflammatory and anti-tyrosinase constituents found in aloe vera leaves. High-speed countercurrent chromatography (HSCCC) is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol. Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents. The chromone compounds with a high performance liquid chromatographic grade (>95%) was isolated through two step HSCCC separations by employing two solvent systems composed of chloroform-methanol-water and dichloromethane-methanol-water at volume ratios of 4/3/2 and 5/4/2, respectively. The chromone was finally identified as cinnamoyl-C-glycoside chromone by ultraviolet (UV), fast atom bombardment mass spectrometry (FAB-MS), nuclear magnetic resonance (1H NMR and 13C NMR).
authors.affiliation: Beijing Key Laboratory of Plant Resources Research and Development, School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Pan
authors.forename: Xueli
authors.initials: X
authors.lastname: Cao
authors.forename: Yinmao
authors.initials: Y
authors.lastname: Dong
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000504:Aloe / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification; D006027:Glycosides; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
chemical_list: C100862:8-(2-O-cinnamoyl(glucopyranosyl))-2-(2-hydroxypropyl)-7-methoxy-5-methylchromone; C438934:C-glycoside; D002867:Chromones; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D009005:Monosaccharides; D010936:Plant Extracts