Construction and expression of recombinant streptolysin-o and preevaluation of its use in immunoassays.
Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.
Velázquez
Blanca
B
Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Massaldi
Hugo
H
Battistoni
Julio
J
Chabalgoity
José A
JA
D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D013290:Streptococcal Infections / Q000175:diagnosis; D013297:Streptococcus pyogenes / Q000276:immunology / Q000302:isolation & purification; D013301:Streptolysins / Q000235:genetics / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D013301:Streptolysins; C025964:streptolysin O; D005982:Glutathione Transferase