Sensitivity of adenylyl cyclase signaling system of the mollusk Anodonta cygnea ganglions to serotonin and adrenergic agonists.
For the first time, the adenylyl cyclase system (ACS) sensitive to biogenic amines in the mollusk Anodonta cygnea ganglions was revealed and characterized. Serotonin and isoproterenol stimulated AC activity and GTP-binding activity of heterotrimeric G-proteins. The AC-stimulating action of serotonin and isoproterenol was blocked by cyproheptadine and adrenergic antagonists, respectively. Using synthetic C-terminal peptides of G-protein alpha-subunit, it was shown that the biogenic amines realized their action on the ACS through different G-protein types: serotonin and isoproterenol activate G(s)-protein, while adrenaline preferably activates G(i)-protein.
Shpakov
A O
AO
Sechenov Institute of Evolutionary Physiology and Biochemistry, RAS, St. Petersburg, Russia. alex_shpakov@list.ru
Shipilov
V N
VN
Bondareva
V M
VM
D000262:Adenylyl Cyclases / Q000502:physiology*; D000322:Adrenergic Agonists / Q000494:pharmacology*; D000818:Animals; D049877:Anodonta / Q000187:drug effects / Q000201:enzymology*; D005724:Ganglia / Q000187:drug effects / Q000201:enzymology*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D000322:Adrenergic Agonists; D017366:Serotonin Receptor Agonists; D000262:Adenylyl Cyclases