Lozenge directly activates argos and klumpfuss to regulate programmed cell death.
We show that reducing the activity of the Drosophila Runx protein Lozenge (Lz) during pupal development causes a decrease in cell death in the eye. We identified Lz-binding sites in introns of argos (aos) and klumpfuss (klu) and demonstrate that these genes are directly activated targets of Lz. Loss of either aos or klu reduces cell death, suggesting that Lz promotes apoptosis at least in part by regulating aos and klu. These results provide novel insights into the control of programmed cell death (PCD) by Lz during Drosophila eye development.
Wildonger
Jill
J
Center for Neurobiology and Behavior, Howard Hughes Medical Institute, Columbia University Medical School, New York, NY 10032, USA.
Sosinsky
Alona
A
Honig
Barry
B
Mann
Richard S
RS
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001665:Binding Sites; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology; D024202:Electrophoretic Mobility Shift Assay; D004843:Epistasis, Genetic; D005136:Eye Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D023281:Genomics / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017956:Photoreceptor Cells, Invertebrate / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; C107732:Klu protein, Drosophila; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C485184:enhanced green fluorescent protein; C099964:lz protein, Drosophila; D049452:Green Fluorescent Proteins; C075638:aos protein, Drosophila