title: Effect of ozone on enamel and dentin bond strength.
abstract: OBJECTIVE
  To evaluate the influence of direct high-dose gaseous ozone application (2100 ppm) on dentin and enamel shear bond strength.
  
  
  METHODS
  Ten bovine enamel and dentin samples per group were pretreated as follows: (I) ozone application (Healozone, KaVo) for 60 s alone or (II) with subsequent application of a fluoride- and xylitol-containing antioxidant (liquid reductant), (III) light-activated bleaching with 35% hydrogen peroxide for 5 min serving as negative control (Hi-Lite, Shofu), and (IV) untreated enamel and dentin (positive control). Specimens were bonded with a functional 3-step adhesive system (Syntac Classic, Ivoclar Vivadent) and restored with a composite (Tetric Ceram, Ivoclar Vivadent) according to the Ultradent method. After storage in water at 37 degrees C for 24 h, shear bond strength was measured using a Zwick universal testing machine. Data were analyzed using ANOVA and Scheffe's post hoc analysis.
  
  
  RESULTS
  In concordance with the existing literature, bleaching resulted in significantly decreased bond strength (p < 0.05) on enamel specimens. No decrease in shear bond strength was detected for ozone-pretreated specimens compared to untreated controls.
  
  
  CONCLUSIONS
  Despite a possible retention of surface and subsurface oxide-related substances during high-dose ozone application, shear bond strength was not impaired. Thus, adhesive restoration placement should be possible immediately after ozone application for cavity disinfection.
authors.affiliation: Clinic for Preventive Dentistry, Periodontology and Cariology, Center for Dental Medicine, University of Zürich, Switzerland. patrick.schmidlin@zzmk.unizh.ch
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Schmidlin
authors.forename: Jörg
authors.initials: J
authors.lastname: Zimmermann
authors.forename: Andreas
authors.initials: A
authors.lastname: Bindl
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D001840:Dental Bonding; D003737:Dental Cavity Preparation; D019551:Dental Disinfectants / Q000633:toxicity*; D003743:Dental Enamel / Q000187:drug effects*; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity; D008422:Materials Testing; D016877:Oxidants / Q000633:toxicity; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D033081:Shear Strength; D018709:Statistics, Nonparametric; D013431:Sulfates / Q000633:toxicity
chemical_list: D019551:Dental Disinfectants; C102653:Hi Lite; D016877:Oxidants; D010083:Oxidants, Photochemical; D013431:Sulfates; D010126:Ozone; D006861:Hydrogen Peroxide