Prepubertal chronic ethanol administration alters TTF-1 and Oct-2 expression in the hypothalamus of female rats.
We found that prolonged administration of ethanol (3 g/kg i.p. at 08:00, once per day) to young female rats starting on postnatal day 24 caused delayed puberty. We further found that prolonged ethanol administration changed the typical hypothalamic expression patterns of TTF-1 and Oct-2 and reduced GnRH mRNA expression. We suggest that these changes may cause the ethanol-induced disturbances in the regulation of GnRH in the hypothalamus and may be responsible for the ethanol-induced reduction in GnRH and LH associated with delayed puberty.
Kim
Hyun Joon
HJ
Department of Anatomy and Neurobiology, Institute of Health Sciences, College of Medicinem, Gyeongsang National University, 92 Chilam-dong, Jinju 660-751, South Korea.
Sohn
Hyun Joon
HJ
Ha
Myung
M
Han
Jae Yoon
JY
Kang
Sang Soo
SS
Choi
Wan Sung
WS
Cho
Gyeong Jae
GJ
D000375:Aging / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002452:Cell Count / Q000379:methods; D002492:Central Nervous System Depressants / Q000008:administration & dosage*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000378:metabolism*; D050813:Octamer Transcription Factor-2; D051381:Rats; D017207:Rats, Sprague-Dawley; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism*
D002492:Central Nervous System Depressants; D004268:DNA-Binding Proteins; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; C489205:Pou2f2 protein, rat; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D007987:Gonadotropin-Releasing Hormone; D000431:Ethanol