Isolates of Trichuris muris elicit different adaptive immune responses in their murine host.
The J and S isolates of Trichuris muris have different infection profiles in C57BL/6 mice; J worms are expelled, S worms survive to chronicity. Building on this, the ability of the J and S isolates to survive, and the quality of the immune response induced was explored in three different strains of mouse. The resistant BALB/c mouse mounted a strong Th2 response against both isolates, which were quickly expelled. The susceptible AKR host mounted a Th1 response and retained both isolates. Despite equivalent worm exposure, mesenteric lymph node cells from AKR mice infected with the S isolate produced significantly higher levels of IL-12 and the intestinal mastocytosis was reduced. IgG1 and IgG2a from S-infected AKR mice recognized low molecular weight antigens not recognized by J-infected mice. Differential expulsion kinetics was observed in the slower-responding C57BL/6 strain; J worms were expelled but S isolate worms were retained. Survival of the S isolate was again associated with elevated IL-12 and decreased Th2 responses. In resistant mouse strains, the outcome of infection is thus dominantly influenced by host genetics. However, in the slower-responding host, isolate-derived factors may play a role in shaping the quality of the adaptive immune response, thus influencing parasite survival.
Johnston
C E
CE
Faculty of Life Sciences, University of Manchester, Manchester, UK.
Bradley
J E
JE
Behnke
J M
JM
Matthews
K R
KR
Else
K J
KJ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017238:Genes, Helminth; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D018664:Interleukin-12 / Q000032:analysis / Q000276:immunology; D007420:Intestine, Large / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D018418:Th2 Cells / Q000276:immunology; D014257:Trichuriasis / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D014258:Trichuris / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D004275:DNA, Ribosomal; D007074:Immunoglobulin G; D018664:Interleukin-12