title: NMR structure and regulated expression in APL cell of human SH3BGRL3.
abstract: SH3 domain binding glutamic acid-rich protein like 3 (SH3BGRL3) is the new member of thioredoxin (TRX) super family, whose posttranslational modified form was identified as tumor necrosis factor alpha (TNF-alpha) inhibitory protein, TIP-B1. In this paper, we determined its solution structure by multi-dimensional nuclear magnetic resonance spectroscopy. The overall structure of human SH3BGRL3 conformed to a TRX-like fold. To understand its function in vivo, the upregulated expression in acute promyelocytic leukemia cell line NB4 at both mRNA and protein level was elucidated. Immunofluorescence and immunohistochemistry staining with monoclonal antibody against SH3BGRL3 demonstrated that it was a cytoplasmic protein in both NB4 cell and human tissues. These results, as a whole, indicate that SH3BGRL3 may function as a regulator in all-trans retinoic acid-induced pathway.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.forename: Peizheng
authors.initials: P
authors.lastname: Zheng
authors.forename: Shuhong
authors.initials: S
authors.lastname: Shen
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Xu
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
authors.forename: Shengnian
authors.initials: S
authors.lastname: Wang
authors.forename: Chongri
authors.initials: C
authors.lastname: Zhu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Tang
authors.forename: Jihui
authors.initials: J
authors.lastname: Wu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yunyu
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D007150:Immunohistochemistry; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009124:Muscle Proteins; C104671:SH3BGR protein, human