title: Increased expression of both constitutive and inducible forms of nitric oxide synthase in the delayed phase of acute experimental testicular torsion.
abstract: To elucidate the roles of both constitutive endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS), and inducible NOS (iNOS) in acute experimental testicular torsion, the expression of iNOS and constitutive eNOS and nNOS were studied in the rat testis with ischemia/reperfusion (I/R) injury. Western blot analysis showed that all three isoforms of NOS increased significantly at 24-48 hr after I/R and declined slightly thereafter. After I/R, immunoreactivity for both iNOS and nNOS was detected, mainly in the interstitial space around damaged tubules, while germ cells in the damaged tubules were immunostained intensely for eNOS. We postulate that increased expression of the three NOS isoforms in the testis after I/R, which might generate nitric oxide, affects delayed germ cell death following I/R via paracrine or autocrine fashion.
authors.affiliation: Department of Veterinary Medicine, Cheju National University, Jeju, South Korea.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Seungjoon
authors.initials: S
authors.lastname: Kim
authors.forename: Fumioki
authors.initials: F
authors.lastname: Yasuzumi
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004790:Enzyme Induction / Q000502:physiology; D006652:Histological Techniques; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D013086:Spermatic Cord Torsion / Q000378:metabolism*; D013737:Testis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D019001:Nitric Oxide Synthase