Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity in rats.
In order to find out the effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity, male rats fed with ethanol 1.6g/kg body weight per day for four weeks were studied. Besides a drastic reduction in body and testis weight, there was decrease in ascorbic acid, reduced glutathione and activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in the testicular tissue of the treated animals. Simultaneously, there was increase in lipid peroxidation and glutathione S-transferase activity. Activities of 3 beta-hydroxy steroid dehydrogenase and 17 beta-hydroxy steroid dehydrogenase were also found decreased in the treated animals. The results indicate that chronic ethanol administration resulted in increase in oxidative stress and decrease in the activities of steroidogenic enzymes in the rat testes.
Maneesh
M
M
Department of Biochemistry, Sikkim Manipal Institute of Medical Sciences, Gangtok 737 102, India.
Jayalekshmi
H
H
Dutta
Sanjiba
S
Chakrabarti
Amit
A
Vasudevan
D M
DM
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D000431:Ethanol; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases