Interleukin-2-dependent regulation of Na/K pump in human lymphocytes.
The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T cells, IL-2 increases both the transport activity of Na/K pump and the content of Na,K-ATPase alpha1 protein in a time-dependent manner. A correlation was found between the long-term elevation in ouabain-sensitive Rb influxes and the increase in alpha1 protein content in late activated T cells. These results suggest that (1) the increased expression of Na,K-ATPase proteins underlie the cell cycle-dependent upregulation of ion pump during T cell transformation, and (2) IL-2 is involved in the regulated expression of Na,K-ATPase in human lymphocytes.
Marakhova
Irina
I
Institute of Cytology RAS, St. Petersburg, Russia. iim@mail.cytspb.rssi.ru
Karitskaya
Inna
I
Aksenov
Nikolay
N
Zenin
Valerii
V
Vinogradova
Tatjana
T
D002453:Cell Cycle / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015759:Ionomycin / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D015240:Phorbol 12,13-Dibutyrate; D015759:Ionomycin; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium