title: Protein profile changes in the human breast cancer cell line MCF-7 in response to SEL1L gene induction.
abstract: The ectopic expression of the gene SEL1L in the human breast carcinoma cell line MCF-7 resulted in a reduction of the aggressive behaviour of these cells in vitro. In addition, in vivo analysis on a series of primary breast carcinomas revealed an association between the SEL1L protein levels and the patient's overall survival. We aimed to find those proteins, associated with SEL1L, which may be involved in modulating the aggressive or invasive behaviour of breast cancer cells. For this purpose, we used both the proteomic and microarray approaches. Image analysis of two-dimensional electropherograms revealed the presence of 27 qualitative and 35 quantitative variations between the MCF7-SEL1L expressing cells compared to control. Mass spectrometry identified 32 changing proteins mostly involved in cytoskeletal and metabolic activities, stress response and protein folding, selenoprotein synthesis and cellular proliferation. Five of these also showed changes in transcript levels, as assessed by Affymetrix microarray analysis. Interestingly, seven proteins: carbonic anhydrase (CA) II, ovarian/breast septin, S100A16 calcium binding protein, 14-3-3 protein sigma, proteasome subunit beta type 6, Hsp60 and protein disulphide-isomerase A3 merit particular attention since they are known to be involved in cancer, in response to cellular stress and in protein folding.
authors.affiliation: Functional Proteomics Laboratory, Department of Molecular Biology, University of Siena, Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bianchi
authors.forename: Cristina
authors.initials: C
authors.lastname: Canton
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.forename: Rosaria
authors.initials: R
authors.lastname: Orlandi
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ménard
authors.forename: Alessandro
authors.initials: A
authors.lastname: Armini
authors.forename: Monica
authors.initials: M
authors.lastname: Cattaneo
authors.forename: Vitaliano
authors.initials: V
authors.lastname: Pallini
authors.forename: Luigi Rossi
authors.initials: LR
authors.lastname: Bernardi
authors.forename: Ida
authors.initials: I
authors.lastname: Biunno
mesh_terms: D000230:Adenocarcinoma; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D003907:Dexamethasone / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D011506:Proteins; D020543:Proteome; C117384:SEL1L protein, human; D003907:Dexamethasone