Morphological and oxidative alterations on Sertoli cells cytoskeleton due to retinol-induced reactive oxygen species.
Retinol (vitamin A) is involved in several cellular processes, like cell division, differentiation, transformation and apoptosis. Although it has been shown that retinol is a limitant factor for all these processes, the precise mechanisms by which retinol acts are still unknown. In the present study we hypothesised that alterations in the cytoskeleton of Sertoli cells induced by retinol supplementation could indicate an adaptive maintenance of its functions, since it plays an important role in the transformation process that we observed. Previous results demonstrated that Sertoli cells treated with retinol showed an oxidative imbalance, that leads the cell to two phenotypes: apoptosis or transformation. Our group has identified characteristics of Sertoli cells transformed by retinol which results in normal cell functions modification. In the present study the actin filament fluorescence assay and the deformation coefficient showed a modification in the morphology induced by retinol. We also observed an oxidative alteration in isolated cytoskeleton proteins and did not show alterations when these proteins are analyzed by electrophoreses. Our results showed an increase in mitochondria superoxide production and a decrease in nitric oxide levels. All results were partially or completely reverted by co-treatment of the antioxidant Trolox. These findings suggest that the cytoskeleton components suffer individual alterations in different levels and that these alterations generate a global phenotype modification and that these processes are probably ROS dependent. We believe that the results from this study indicate an adaptation of the cytoskeleton to oxidative imbalance since there was not a loss of its function.
de Oliveira
Ramatis Birnfeld
RB
Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, ICBS-Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2600 anexo, CEP 90035-003, Porto Alegre, Brasil. ramatusoliveira@yahoo.com.br
Klamt
Fábio
F
Castro
Mauro A A
MA
Polydoro
Manuela
M
Zanotto Filho
Alfeu
A
Gelain
Daniel Pens
DP
Dal-Pizzol
Felipe
F
Moreira
José Cláudio Fonseca
JC
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D018384:Oxidative Stress; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D014801:Vitamin A / Q000494:pharmacology*
D000199:Actins; D003598:Cytoskeletal Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; D014801:Vitamin A