A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase.
Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.
Manukhov
Ilya V
IV
State Research Institute of Genetic and Selection of Industrial Microorganisms, 1st Dorozhnii pr. 1, Moscow 117545, Russia.
Mamaeva
Daria V
DV
Rastorguev
Sergei M
SM
Faleev
Nicolai G
NG
Morozova
Elena A
EA
Demidkina
Tatyana V
TV
Zavilgelsky
Gennadii B
GB
D001483:Base Sequence; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016971:Citrobacter freundii / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase