Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein.
The storage mite, Tyrophagus putrescentiae, is one of the important causes of allergic disorders. Fifteen allergenic components were demonstrated in storage mite by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, but only the group 2 allergen Tyr p 2 has been cloned and characterized. In this study, we attempted to identify and characterize new allergens from T. putrescentiae, which is a dominant species of storage mite in Korea. Expressed sequence tags were analyzed to identify possible storage mite allergens, and the cDNA sequence encoding a protein homologous to fatty acid binding protein, a mite group 13 allergen, was identified and named Tyr p 13. Its deduced amino acid sequence showed 61.1 to 85.3% identity with other mite group 13 allergens. The recombinant protein was expressed in Escherichia coli using a pET 28b vector system, and its allergenicity was investigated by enzyme-linked immunosorbent assay (ELISA). The recombinant allergen was detected in 5 of 78 (6.4%) T. putrescentiae-positive sera tested, and it inhibited 61.9% of immunoglobulin E binding to crude extract at an inhibitor concentration of 10 mug/ml by inhibition ELISA using serum from the patient who showed the strongest reaction by ELISA. In this study, a novel allergen was identified in T. putrescentiae. This allergen could be helpful for more-detailed characterizations of storage mite allergy.
Jeong
Kyoung Yong
KY
Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
Kim
Woo Kyung
WK
Lee
Jae Sik
JS
Lee
Jongweon
J
Lee
In-Yong
IY
Kim
Kyu-Earn
KE
Park
Jung Won
JW
Hong
Chein-Soo
CS
Ree
Han-Il
HI
Yong
Tai-Soon
TS
D040041:Acaridae / Q000276:immunology*; D000293:Adolescent; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007073:Immunoglobulin E / Q000276:immunology*; D007723:Korea; D008297:Male; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins; D012689:Sequence Homology, Nucleic Acid
D000485:Allergens; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D011506:Proteins; D011994:Recombinant Proteins; C110921:Tyr p 2 protein, Tyrophagus putrescentiae; D007073:Immunoglobulin E