An efficient solid phase synthesis of 5'-phosphodiester and phosphoramidate monoester nucleoside analogues.
An easy and efficient strategy to obtain libraries of 5'-phosphodiester and 5'-phosphoramidate monoester nucleoside analogues in a highly pure form has been developed, starting from a new nucleoside based solid support. The nucleoside scaffold has been anchored through a 5'-phosphodiester linkage to Tentagel HL resin, functionalized with a 3-chloro-4-hydroxyphenylacetic linker. The solid phase synthesis of small libraries of 5'-phosphodiester and 5'-phosphoramidate monoester thymidine analogues is also reported.
De Napoli
Lorenzo
L
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Complesso Universitario di Monte S. Angelo, via Cynthia, 4 I-80126, Napoli, Italy.
Di Fabio
Giovanni
G
D'Onofrio
Jennifer
J
Montesarchio
Daniela
D
D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry; D004952:Esters / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry
D000577:Amides; D004952:Esters; D009705:Nucleosides; D009943:Organophosphorus Compounds; D010756:Phosphoric Acids; C011067:phosphoramidic acid