Simultaneous retention index analysis of urinary amino acids and carboxylic acids for graphic recognition of abnormal state.
Simultaneous profiling analysis of urinary amino acids (AAs) and carboxylic acids (CAs) was combined with retention index (I) analysis for graphic recognition of abnormal metabolic state. The temperature-programmed I values of the AA and CA standards measured as ethoxycarbonyl (EOC)/methoxime (MO)/tert-butyldimethylsilyl (TBDMS) derivatives were used as the reference I values. Urine samples were subjected to the sequential EOC, MO and TBDMS reactions for the analysis by gas chromatography (GC) and GC-mass spectrometry. The complex GC profiles were then transformed into their respective I patterns in bar graphic forms by plotting the normalized peak area ratios (%) of the identified AAs and CAs against their reference I values as the identification numbers. When the present method was applied to infant urine specimens from normal controls and patients with inherited metabolic diseases such as phenylketonuria, maple syrup urine disease, methylmalonic aciduria or isovaleric aciduria, each I pattern of bar graph more distinctly displayed quantitative abundances of urinary AAs and CAs in qualitative I scale, thus allowing graphic discrimination between normal and abnormal states.
Paik
Man-Jeong
MJ
Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University, 300 Chunchun-dong, Suwon, Kyunggi-do 440-746, South Korea.
Lee
Hong-Jin
HJ
Kim
Kyoung-Rae
KR
D000081:Acetamides; D000596:Amino Acids / Q000652:urine*; D002264:Carboxylic Acids / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000379:methods*; D005463:Fluoroacetates; D045782:Hemiterpenes; D006801:Humans; D006898:Hydroxylamines / Q000737:chemistry; D007223:Infant; D008375:Maple Syrup Urine Disease / Q000652:urine; D008661:Metabolism, Inborn Errors / Q000652:urine*; D008764:Methylmalonic Acid / Q000652:urine; D017646:Organosilicon Compounds / Q000737:chemistry; D010421:Pentanoic Acids / Q000652:urine; D010661:Phenylketonurias / Q000652:urine; D014269:Trifluoroacetic Acid / Q000737:chemistry
D000081:Acetamides; D000596:Amino Acids; D002264:Carboxylic Acids; D005463:Fluoroacetates; D045782:Hemiterpenes; D006898:Hydroxylamines; D017646:Organosilicon Compounds; D010421:Pentanoic Acids; C008216:isovaleric acid; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D008764:Methylmalonic Acid; C005214:methoxyamine; D014269:Trifluoroacetic Acid