L7/Pcp-2-specific expression of Cre recombinase using knock-in approach.
We report a knock-in mouse expressing Cre recombinase from the translational initiation site (ATG) of the endogenous L7/Pcp-2 gene. The resulting Cre expression matches the pattern of L7/Pcp-2 expression that is restricted to cerebellar Purkinje cells and retinal cells. Moreover, the Cre mouse showed no significant behavioral abnormality. Thus, our novel Cre mouse can be used for generation of Purkinje cells and retinal cell-specific gene expression and/or knockout in mouse using the Cre/loxP system.
Saito
Hiromitsu
H
Department of Animal Genomics, Functional Genomics Institute, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
Tsumura
Hideki
H
Otake
Seiichi
S
Nishida
Akihiro
A
Furukawa
Takahisa
T
Suzuki
Noboru
N
D000818:Animals; D017930:Genes, Reporter; D020662:Guanine Nucleotide Exchange Factors; D007150:Immunohistochemistry; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009479:Neuropeptides / Q000235:genetics*
D020662:Guanine Nucleotide Exchange Factors; D009479:Neuropeptides; C104971:Pcp2 protein, mouse; C045073:Cre recombinase; D019426:Integrases