To use MIBI or not to use MIBI? That is the question when assessing tumour cells.
99mTc-sestamibi (MIBI) is a well-known tumour imaging agent. Its retention within tumour cell mitochondria is related to perfusion and to the magnitude of the electrical gradient, reflecting cell viability. Several internal cell factors modulate this uptake; for example, multidrug resistance membrane proteins (Pgp and MRP1) and anti-apoptotic BCl-2 protein of the outer mitochondrial membrane can limit retention of MIBI. At the early stage of cell apoptosis, the electrical driving forces of MIBI uptake are impaired, and influx and accumulation are reduced. It seems clear that MIBI can be used before treatment to detect drug resistance, assess anti-apoptotic status and predict treatment efficacy. Although it has been suggested that MIBI might be used to monitor tumour response to treatment, MIBI is unable to differentiate tumours with ongoing apoptosis from those developing drug resistance.
Moretti
Jean-Luc
JL
UPRES 2360 Ciblage et Imagerie Fonctionnelle de la Progression Tumorale, Faculté de Médecine, Bobigny, France. jean-luc.moretti@avc.ap-hop-paris.fr
Hauet
Nathalie
N
Caglar
Meltem
M
Rebillard
Olivier
O
Burak
Zeynep
Z
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D018432:Drug Resistance, Multiple; D006801:Humans; D008564:Membrane Potentials; D008928:Mitochondria / Q000473:pathology; D008954:Models, Biological; D009369:Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging* / Q000473:pathology; D010477:Perfusion; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011877:Radionuclide Imaging / Q000295:instrumentation* / Q000379:methods*; D017256:Technetium Tc 99m Sestamibi; D016896:Treatment Outcome
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D019253:Proto-Oncogene Proteins c-bcl-2; D017256:Technetium Tc 99m Sestamibi