Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc.
An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299(T) was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, (1)H and (13)C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:
Kilcoyne
Michelle
M
Department of Chemistry, National University of Ireland, Galway.
Shashkov
Alexander S
AS
Perepelov
Andrei V
AV
Nazarenko
Evgeny L
EL
Gorshkova
Raisa P
RP
Ivanova
Elena P
EP
Widmalm
Göran
G
Savage
Angela V
AV
D002240:Carbohydrate Sequence; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D020592:Shewanella / Q000737:chemistry* / Q000145:classification*
C504608:4-acetamido-4,6-dideoxygalactose; D019081:O Antigens; D005643:Fucose