A cryptic Rab1-binding site in the p115 tethering protein.
Small GTPases and coiled-coil proteins of the golgin family help to tether COPI vesicles to Golgi membranes. At the cis-side of the Golgi, the Rab1 GTPase binds directly to each of three coiled-coil proteins: p115, GM130, and as now shown, Giantin. Rab1 binds to a coiled-coil region within the tail domain of p115 and this binding is inhibited by the C-terminal, acidic domain of p115. Furthermore, GM130 and Giantin bind to the acidic domain of p115 and stimulate p115 binding to Rab1, suggesting that p115 binding to Rab1 is regulated. Regulation of this interaction by proteins such as GM130 and Giantin may control the membrane recruitment of p115 by Rab1.
Beard
Matthew
M
Department of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA.
Satoh
Ayano
A
Shorter
James
J
Warren
Graham
G
D000818:Animals; D000906:Antibodies; D001324:Autoantigens; D001665:Binding Sites / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D051381:Rats; D033921:Vesicular Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D020761:rab1 GTP-Binding Proteins / Q000378:metabolism*
D000906:Antibodies; D001324:Autoantigens; D000077614:Golgi Matrix Proteins; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; C087574:macrogolgin; C091580:vesicular transport factor p115; D020761:rab1 GTP-Binding Proteins