Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins.
Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition.
Guo
Tian-Ying
TY
State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China. tyguo@nankai.edu.cn
Xia
Yong-Qing
YQ
Wang
Jin
J
Song
Mou-Dao
MD
Zhang
Bang-Hua
BH
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D008422:Materials Testing; D008863:Microspheres; D016062:Porosity; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties; D014462:Ultrafiltration / Q000379:methods*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D006454:Hemoglobins; C016680:polyacrylamide gels; D012710:Serum Albumin, Bovine; D048271:Chitosan