Survival factor-like activity of small peptides in hybridoma and CHO cells cultures.
Synthetic peptides containing three to six amino acid residues were previously shown to improve key parameters of monoclonal antibody-producing mouse hybridoma cultures. The aim of the current work was to investigate whether small peptides also exert analogous beneficial impact on a CHO-K1-derived cell line (XMK-111-10) engineered for production of the human model glycoprotein SEAP (secreted alkaline phosphatase). Similar to hybridoma cultures, growth and SEAP production profiles of CHO XMK-111-10 were modulated by peptides. Both viable cell density and SEAP production were increased by tetraalanine or by a fraction of wheat gluten hydrolysate. Whereas tetraglycine increased the peak viable cell density, the growth-suppressing tripeptide Gly-Lys-Gly significantly boosted SEAP production. All peptide-supplemented cultures showed slight improvement of culture viability during the decline phase of the batch cultures, suggesting a survival factor-like activity of the peptides.
Franĕk
Frantisek
F
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Radiova 1, CZ-10227 Prague 10, Czech Republic. franek@biomed.cas.cz
Fussenegger
Martin
M
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000187:drug effects; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D010455:Peptides; C019529:polyalanine; D000469:Alkaline Phosphatase