Battery of tests for profiling abnormalities of vitamin K-dependent coagulation factors in drug-toxicity studies in rats.
A battery of simple tests for profiling abnormalities of vitamin K-dependent coagulation factors encountered in drug-toxicity studies was verified in rats treated with warfarin (3 and 10 mg/kg, p.o). The thrombotest, or hepaplastin-test, is useful as a follow-up test after routine screening tests for coagulation abnormalities based on PT and APTT, to rule out other coagulation-factor abnormalities. Measurement of coagulation factor activities (factors II, VII, IX and X) using factor-deficient human plasmas provides direct evidence of decreased activities of vitamin K-dependent factors. Furthermore, Echis carinatus venom coagulation time, together with factor II activity, allows us to confirm the generation of PIVKA-II.
Kurata
Masaaki
M
Worldwide Safety Sciences, Pfizer Global Research and Development, Nagoya Laboratories, Pfizer Inc, Aichi, Japan.
Iidaka
Takeshi
T
Yamasaki
Naoko
N
Sasayama
Yukiko
Y
Hamada
Yoshimasa
Y
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001778:Blood Coagulation Disorders / Q000097:blood / Q000139:chemically induced; D001779:Blood Coagulation Factors / Q000032:analysis*; D001780:Blood Coagulation Tests / Q000379:methods*; D008297:Male; D011498:Protein Precursors / Q000097:blood*; D011516:Prothrombin; D051381:Rats; D011919:Rats, Inbred Strains; D018675:Toxicity Tests; D014812:Vitamin K; D014859:Warfarin
D015415:Biomarkers; D001779:Blood Coagulation Factors; D011498:Protein Precursors; D014812:Vitamin K; C008208:acarboxyprothrombin; D014859:Warfarin; D011516:Prothrombin