Recognition of CG inversions in DNA triple helices by methylated 3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues.
Substituted 3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues have been synthesised from 5-alkynyl-uridine derivatives, incorporated into triplex forming oligonucleotides (TFOs) and found to selectively bind CG inversions with enhanced affinity compared to T.
Ranasinghe
Rohan T
RT
School of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ.
Rusling
David A
DA
Powers
Vicki E C
VE
Fox
Keith R
KR
Brown
Tom
T
D020029:Base Pairing; D003596:Cytosine / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000032:analysis* / Q000737:chemistry; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011744:Pyrimidinones / Q000138:chemical synthesis / Q000737:chemistry*; D013941:Thymine / Q000032:analysis / Q000737:chemistry
D009705:Nucleosides; D009841:Oligonucleotides; D011744:Pyrimidinones; C051802:triplex DNA; D006147:Guanine; D003596:Cytosine; D004247:DNA; D013941:Thymine