Expression pattern of voltage-dependent calcium channel alpha1 and beta subunits in adrenal gland of N-type Ca2+ channel alpha1B subunit gene-deficient mice.
The Ca2+ channel alpha1B subunit is a pore-forming component capable of generating N-type Ca2+ channel activity. Although N-type Ca2+ channel plays a role in a variety of neuronal functions, alpha1B-deficient mice exhibit normal life span without apparent abnormalities of behavior, histology or plasma norepinephrine level, presumably owing to compensation by some other Ca2+ channel alpha1 or beta subunit. In this study, we studied the levels of alpha1A, alpha1C, alpha1D, C1E, beta1, beta2, beta3 and beta4 mRNAs in adrenal gland of alpha1B-deficient mice. The alpha1A mRNA in homozygous mice was expressed at higher level than in wild or heterozygous mice, but no difference in the expression levels of alpha1c, alpha1D, alpha1E, beta1, beta2, beta3 and beta4 was found among wild, heterozygous and homozygous mice. The protein level of alpha1A in homozygous mice was also expressed at higher level than in wild or heterozygous mice. To examine whether increased expression is induced by cis-regulatory element within 5'-upstream region of alpha1A gene, we examined lacZ expression in alpha1B-deficient x alpha1A6.3-lacZ mice (carrying a 6.3-kb 5'-upstream fragment of alpha1A gene fused to E. coli lacZ reporter gene), which express lacZ in medullar chromaffin cells, but not in cortex. The levels of lacZ expression in homozygous alpha1B-deficient x alpha1A6.3-lacZ mice were higher than in wild or heterozygous mice. Therefore, a possible explanation of the normal behavior and plasma norepinephrine level of alpha1B-deficient mice is that compensation by alpha1A subunit occurs and that 6.3-kb 5'-upstream region of alpha1A gene contains enhancer cis-element(s) for compensation in adrenal medulla chromaffin cells.
Takahashi
Eiki
E
Tsukuba Research Laboratory, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. e2-takahashi@hhc.eisai.co.jp
Nagasu
Takeshi
T
D000311:Adrenal Glands / Q000166:cytology / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D015153:Blotting, Western; D015220:Calcium Channels / Q000235:genetics; D020746:Calcium Channels, L-Type / Q000235:genetics; D020864:Calcium Channels, N-Type / Q000235:genetics* / Q000378:metabolism; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins / Q000235:genetics; D019439:Chromaffin Cells / Q000502:physiology; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009638:Norepinephrine / Q000097:blood; D021122:Protein Subunits; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D012045:Regulatory Sequences, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
C107597:CACNB2 protein, human; C096073:CACNB3 protein, human; C491367:Cacna1a protein, rat; C498916:Cacna1e protein, mouse; C491530:Cacnb4 protein, mouse; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; D001616:beta-Galactosidase; D009638:Norepinephrine