title: Fast changes in expression of expansin gene and leaf extensibility in osmotically stressed maize plants.
abstract: Adding PEG to the nutrient medium of maize (Zea mays L., hybrid Harkovskaya 310 MV) plants arrested the growth of their leaves initially but in 40-50 min growth resumed. This coincided with and was obviously due to a gradual increase in extensibility of the primary leaf suggested by changes in its extension rate, which was induced by adding a counterweight to inductive electromechanical position sensor. Specificity of gene probe for expansins was confirmed by sequencing cDNA and its comparison with literature data. Dot-blot analysis showed an increase in transcript level of expansin genes induced by PEG treatment. Thus gene-specific regulation of expansin mRNA pools likely contributes to fast adjustment of cell wall-loosening under conditions of water deficit.
authors.affiliation: Institute of Biology, Ufa Scientific Center of Russian Academy of Sciences, pr. Octyabrya, 69, Ufa 450054, Russia.
authors.forename: Inna B
authors.initials: IB
authors.lastname: Sabirzhanova
authors.forename: Boris E
authors.initials: BE
authors.lastname: Sabirzhanov
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Chemeris
authors.forename: Dmitri S
authors.initials: DS
authors.lastname: Veselov
authors.forename: Guzel R
authors.initials: GR
authors.lastname: Kudoyarova
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D011092:Polyethylene Glycols / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; C095825:expansin protein, plant; D011092:Polyethylene Glycols