title: [Impact of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in treatment of acute cholangitis in rats].
abstract: OBJECTIVE
  To investigate the effects of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in the treatment of acute cholangitis.
  
  
  METHODS
  Sixty SD rats were randomly divided into group A (untreated group, acute cholangitis was induced, n=20), group B (treatment group, acute cholangitis was induced and treated with antipyretic and purgative herbs, n=20) and group C (sham operation group, n=20). At the third or fifth day after operation, the rats were sacrificed and sampled. The serum endotoxin, cytokines and inflammatory mediators were tested and the numbers of labeled bacteria in the liver, spleen and mesenteric lymph nodes translocated from the gut were assayed.
  
  
  RESULTS
  As compared with group A, the serum content of endotoxin, IL-6, IL-8, TNF-alpha, CRP and NO was significantly lower and that of IL-2 was significantly higher, and the translocated numbers of labeled bacteria from gut were reduced in both group B and group C (P<0.01).
  
  
  CONCLUSIONS
  Antipyretic and purgative herbs can play therapeutic roles in the treatment of acute biliary tract infections, including the protection of intestinal mucosal barrier from bacterial translocation, reduction of serum endotoxin content and regulation of inflammatory response.
authors.affiliation: Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. zjzzzq@sina.com
authors.forename: Jing-Zhe
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Ju
authors.initials: J
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
authors.forename: En-Wei
authors.initials: EW
authors.lastname: Ma
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Gu
authors.forename: Pei-Ting
authors.initials: PT
authors.lastname: Zhu
mesh_terms: D000208:Acute Disease; D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002761:Cholangitis / Q000188:drug therapy* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004926:Escherichia coli / Q000502:physiology; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007413:Intestinal Mucosa / Q000382:microbiology / Q000503:physiopathology*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha