title: [Genotoxicity comparison between gasoline- and methanol-fueled exhaust by TK gene mutation assay].
abstract: Methanol fuel is a most promising substitute for gasoline. It is scarcely reported about methanol-fueled exhaust on the health effect, neither about genotoxicity research between methanol- and gasoline-fueled exhaust. In the present study, the two kinds of exhaust were sampled directly from tailpipe at the same type bus, the same state, L5178Y thymidine kinase (TK) gene mutation assay was used to investigate their genotoxicity at the same dose range, and compared with micronucleus and comet assay. The results showed that the genotoxicity of gasoline-fueled exhaust is stronger than that of methanol-fueled exhaust, while the cytotoxicity of methanol-fueled exhaust is stronger than that of gasoline-fueled exhaust at dose range. The study demonstrated that L5178Y TK gene mutation assay is more sensitive than micronucleus and comet assay.
authors.affiliation: West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Li
authors.initials: L
authors.lastname: Zhan
authors.forename: Zunzhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xianggui
authors.initials: X
authors.lastname: Zeng
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Gou
authors.forename: Chuan
authors.initials: C
authors.lastname: Lin
authors.forename: Chunhua
authors.initials: C
authors.lastname: Cai
authors.forename: Xi
authors.initials: X
authors.lastname: Shao
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Shao
authors.forename: Desheng
authors.initials: D
authors.lastname: Wu
mesh_terms: D005742:Gasoline / Q000009:adverse effects*; D006801:Humans; D000432:Methanol / Q000009:adverse effects*; D018986:Motor Vehicles; D009152:Mutagenicity Tests; D009154:Mutation; D013937:Thymidine Kinase / Q000235:genetics*; D001335:Vehicle Emissions / Q000009:adverse effects*
chemical_list: D005742:Gasoline; D001335:Vehicle Emissions; D013937:Thymidine Kinase; D000432:Methanol