Interaction between yeast mitochondrial and nuclear genomes: null alleles of RTG genes affect resistance to the alkaloid lycorine in rho0 petites of Saccharomyces cerevisiae.
Some nuclear genes in Saccharomyces cerevisiae (S. cerevisiae) respond to signals from the mitochondria in a process called by Butow (Cell Death Differ. 9 (2002) 1043-1045) retrograde regulation. Expression of these genes is activated in cells lacking mitochondrial function by involvement of RTG1, RTG2 and RTG3 genes whose protein products bind to "R-boxes" in the promoter region; RTG2p is a cytoplasmic protein. Since S. cerevisiae rho0 strains, lacking the entire mitochondrial genome, are resistant to lycorine, an alkaloid extracted from Amaryllis plants, it could be hypothesized that in rho0 cells the dysfunctional mitochondrial status stimulates overexpression of nuclear genes very likely involved in both nuclear and mitochondrial DNA replication. In this report we show that the resistance of rho0 cells to lycorine is affected by the deletion of RTG genes.
Del Giudice
Luigi
L
Istituto di Genetica e Biofisica Adriano Buzzati-Traverso, Consiglio Nazionale delle Ricerche, Napoli, Italy. delgiudi@igb.cnr.it
Massardo
Domenica Rita
DR
Pontieri
Paola
P
Wolf
Klaus
K
D000483:Alleles; D047151:Amaryllidaceae Alkaloids / Q000494:pharmacology; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D004271:DNA, Fungal / Q000096:biosynthesis; D004272:DNA, Mitochondrial / Q000235:genetics*; D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000096:biosynthesis; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D005947:Glucose / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D009154:Mutation; D010617:Phenanthridines / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012331:RNA, Fungal / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
D047151:Amaryllidaceae Alkaloids; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010617:Phenanthridines; D011500:Protein Synthesis Inhibitors; D012331:RNA, Fungal; C079378:RTG1 protein, S cerevisiae; C079379:RTG2 protein, S cerevisiae; C104438:RTG3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D003513:Cycloheximide; C015330:lycorine; D005947:Glucose