Differences in heat resistance among pathogenic Yersinia enterocolitica depended on growth temperature and serotype.
To gain a better understanding about the effect of growth temperature on heat resistance of Yersinia enterocolitica, we determined decimal reduction times at 60 degrees C (D60-values) for O:3; O:5,27; O:8; and O:9 strains harboring virulence plasmid coding for Yersinia outer membrane protein and experimentally virulence plasmid-deleted strains after they were grown to stationary phase at 7, 25, or 37 degrees C. Bacteria were inoculated into Trypticase soy broth and were incubated at several temperatures. D60-values of O:3; O:5,27; and O:8 strains were larger when they were grown at 37 degrees C than at 7 or 25 degrees C, despite the presence or absence of virulence plasmids. However, similar D60-values were observed in O:9 strains, despite growth at 7, 25, or 37 degrees C. The results indicate two types of Y. enterocolitica strains, growth temperature-dependent and -independent, and a Yersinia outer membrane protein that is not directly involved in growth temperature-dependent heat resistance.
Hayashidani
H
H
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan. eisei@cc.tuat.ac.jp
Hara-Kudo
Y
Y
Kinoshita
S
S
Saeki
K
K
Okatani
A T
AT
Nomura
Y
Y
Kumagai
S
S
D001425:Bacterial Outer Membrane Proteins / Q000187:drug effects* / Q000378:metabolism; D005516:Food Microbiology; D006358:Hot Temperature / Q000009:adverse effects*; D010957:Plasmids; D012703:Serotyping; D013696:Temperature; D013997:Time Factors; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity
D001425:Bacterial Outer Membrane Proteins