title: Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system.
abstract: Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA.
authors.forename: David R M
authors.initials: DR
authors.lastname: Graham
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Garnham
authors.forename: Qin
authors.initials: Q
authors.lastname: Fu
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Robbins
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Van Eyk
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D002021:Buffers; D015180:Electrophoresis, Gel, Two-Dimensional / Q000191:economics / Q000379:methods*; D015497:HIV-1 / Q000737:chemistry; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000737:chemistry; D040901:Proteomics; D011817:Rabbits; D044967:Serum; D014020:Tissue Extracts / Q000737:chemistry; D014325:Tromethamine / Q000031:analogs & derivatives*
chemical_list: D001798:Blood Proteins; D002021:Buffers; D014020:Tissue Extracts; D014325:Tromethamine; C026272:Bistris