title: Gamma interferon levels and antibody production induced by two PvMSP-1 recombinant polypeptides are associated with protective immunity against P.vivax in Aotus monkeys.
abstract: Effector mechanisms responsible for providing protective immunity against Plasmodium vivax (Pv) infection were examined in Aotus monkeys vaccinated with two Pv Merozoite Surface Protein-1 (PvMSP-1) recombinant polypeptides that had previously been shown to protect vaccines against parasite challenge. Vaccine efficacy was reproducible in this trial, showing that one out of the five monkeys immunised with the recombinant protein mixture was partially protected while three others controlled parasitaemia. Antibodies reactive to the parasite's native proteins, the recombinant polypeptides and peptides spanning both recombinant fragments were detected in most vaccinees. Despite substantial Peripheral Blood Mononuclear Cell (PBMC) antigen-specific cellular proliferation not being detected, high rPvMSP-1(20) specific gamma interferon (IFN-gamma) production was found in the three animals that controlled parasitaemia. Altogether the results suggest that antibody titres and antigen-specific IFN-gamma production mediate protective immunity against P. vivax.
authors.affiliation: Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Carrera 50 # 26-00, Bogota, Colombia.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Barrero
authors.forename: Gabriela
authors.initials: G
authors.lastname: Delgado
authors.forename: Adriana Y
authors.initials: AY
authors.lastname: Sierra
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Silva
authors.forename: Carlos
authors.initials: C
authors.lastname: Parra-Lopez
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016645:Aotidae; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D017780:Malaria Vaccines / Q000276:immunology*; D016780:Malaria, Vivax / Q000517:prevention & control*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D018512:Parasitemia; D011994:Recombinant Proteins / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D007371:Interferon-gamma