title: Carbon monoxide signaling in promoting angiogenesis in human microvessel endothelial cells.
abstract: Heme oxygenase isoforms (HO-1/HO-2) catalyze the conversion of heme to carbon monoxide (CO) and bilirubin. In this study, HO-1-deficient endothelial cells were transduced with HO-1 in the antisense orientation to determine whether supplementation with CO or bilirubin would regulate cell proliferation and angiogenesis. Western blotting, enzyme activity, CO and prostaglandin E(2) (PGE(2)) production, and cell-cycle analysis were used to assess transgenic expression and functionality of the recombinant protein. A Matrigel matrix was used for assessment of in vitro capillary formation. Transduction with HO-1 antisense resulted in decreased capillary formation, cell proliferation, and cell-cycle progression, and increased PGE(2) production compared with control. HO-1 deficiency was also associated with increased expression of p21 and p27, but had no significant effect on p16 and p53. We also compared two different CO donors for their ability to rescue angiogenesis. Compared with control, HO-1-deficient endothelial cells showed increased angiogenesis following tricarbonyldichlororuthenium( II) dimer ([Ru(CO)(3)Cl(2)](2)) (CORM-1) starting at 50 microM, whereas tricarbonylchloro(glycinato) ruthenium(II) (CORM-3), starting at 25 microM, was a potent enhancer of angiogenesis. The addition of bilirubin did not restore angiogenesis. These data suggest that HO-mediated angiogenesis and cell proliferation were dependent on HO-1- and not HO-2-derived CO.
authors.affiliation: Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Li Volti
authors.forename: David
authors.initials: D
authors.lastname: Sacerdoti
authors.forename: Bhavani
authors.initials: B
authors.lastname: Sangras
authors.forename: Angelo
authors.initials: A
authors.lastname: Vanella
authors.forename: Alexandre
authors.initials: A
authors.lastname: Mezentsev
authors.forename: Giovanni
authors.initials: G
authors.lastname: Scapagnini
authors.forename: John R
authors.initials: JR
authors.lastname: Falck
authors.forename: Nader G
authors.initials: NG
authors.lastname: Abraham
mesh_terms: D002248:Carbon Monoxide / Q000378:metabolism*; D049109:Cell Proliferation; D004247:DNA / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D051547:Heme Oxygenase-1; D006801:Humans; D008565:Membrane Proteins; D008833:Microcirculation; D018919:Neovascularization, Physiologic / Q000187:drug effects; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D042461:Vascular Endothelial Growth Factor A; D002248:Carbon Monoxide; D004247:DNA; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1