Leishmanization: use of an old method for evaluation of candidate vaccines against leishmaniasis.
To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.
Khamesipour
Ali
A
Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, P.O. Box 14155-6383, Tehran 14166, Iran. khamesipour_ali@yahoo.com
Dowlati
Yahya
Y
Asilian
Ali
A
Hashemi-Fesharki
Reza
R
Javadi
Amir
A
Noazin
Sassan
S
Modabber
Farrokh
F
D000328:Adult; D000818:Animals; D006801:Humans; D007492:Iran; D018320:Leishmania major / Q000276:immunology* / Q000472:pathogenicity; D016773:Leishmaniasis, Cutaneous / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D016052:Protozoan Vaccines / Q000009:adverse effects / Q000276:immunology*; D012882:Skin Tests; D014774:Virulence
D016052:Protozoan Vaccines