Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis.
The performance of the nitrate reductase assay was evaluated in a multicenter laboratory study to detect resistance of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin, isoniazid, ethambutol and streptomycin using a set of coded isolates. Compared with the gold standard proportion method on Löwenstein-Jensen medium, the assay was highly accurate in detecting resistance to rifampicin, isoniazid and ethambutol with an accuracy of 98%, 96.6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities.
Martin
Anandi
A
Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B-2000, Belgium. amartin@itg.be
Montoro
Ernesto
E
Lemus
Dihadenys
D
Simboli
Norberto
N
Morcillo
Nora
N
Velasco
Maritza
M
Chauca
José
J
Barrera
Lucía
L
Ritacco
Viviana
V
Portaels
Françoise
F
Palomino
Juan Carlos
JC
D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology*; D050901:Nitrate Reductase / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology
D000995:Antitubercular Agents; D050901:Nitrate Reductase