title: Use of different PCR primers and gastric biopsy tissue from CLO test for the detection of Helicobacter pylori.
abstract: Four different DNA loci were assessed for the detection of H. pylori by PCR on gastric biopsy specimens. PCR, with a primer specific 860 bp DNA fragment, was the most sensitive, with a detection limit of 0.02 pg H. pylori DNA, corresponding to approximately 10 organisms. Nested-PCR of the 860-bp DNA fragment was 10-fold more sensitive than single-step PCR. The sensitivity and specificity of the four PCR methods, in comparison to the results obtained from histology and the urease test, are as follows: 80.7% and 76% for the hpaA gene; 100% and 76% for the 16S rRNA gene; 84.6% and 80.0% for the 860-bp DNA fragment; 61.5% and 84.0% for the ureC (glmM) gene, respectively. The sensitivity of nested-PCR for the 860-bp DNA fragment was 100%. This nested-PCR gave positive results for eight specimens which were negative by conventional methods. PCR can be performed on gastric biopsy specimens obtained from the CLO test.
authors.affiliation: Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailand. rhxxo028@chiangmai.ac.th
authors.forename: Sukanya
authors.initials: S
authors.lastname: Linpisarn
authors.forename: Chuchart
authors.initials: C
authors.lastname: Koosirirat
authors.forename: Kunrunya
authors.initials: K
authors.lastname: Prommuangyong
authors.forename: Warissara
authors.initials: W
authors.lastname: Suwan
authors.forename: Nirush
authors.initials: N
authors.lastname: Lertprasertsuke
authors.forename: Kannika
authors.initials: K
authors.lastname: Phornphutkul
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001706:Biopsy; D016099:Endoscopy, Gastrointestinal; D005753:Gastric Mucosa / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D014510:Urease