title: CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules.
abstract: CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8 kb on chromosome 16q22.1. Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis.
authors.affiliation: Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, 38 Xueyuan Road, Beijing 100083, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Rui
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
authors.forename: Peiguo
authors.initials: P
authors.lastname: Ding
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhu
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qini
authors.initials: Q
authors.lastname: Gan
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D018925:Chemokines / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D018076:DNA, Complementary; D006801:Humans; D007150:Immunohistochemistry; D062792:MARVEL Domain-Containing Proteins; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: C505071:CMTM2 protein, human; D018925:Chemokines; D018076:DNA, Complementary; D062792:MARVEL Domain-Containing Proteins