Gicerin/CD146 is involved in neurite extension of NGF-treated PC12 cells.
Gicerin/CD146 is a cell adhesion molecule, which belongs to the immunoglobulin (Ig) superfamily. We have reported that it has a homophilic binding activity, which participates in the neurite extension from embryonic neurons. To elucidate how gicerin is involved in the neurite extension mechanism, we employed PC12 cells, which expresses gicerin/CD146. PC12 cells extend longer neurites by nerve growth factor (NGF) on gicerin substrate than on without gicerin substrate, which indicates that gicerin participates in neurite extension by NGF. We also found that the expression of gicerin in PC12 cells is induced by NGF. Over-expression of gicerin also promotes neurite extension by gicerin-gicerin homophilic interaction. These findings suggested that increase of gicerin expression by NGF promotes the gicerin-gicerin homophilic interaction resulting in the neurite extension.
Taira
Eiichi
E
Department of Pharmacology, Iwate Medical School, Uchimaru, Morioka, Iwate, Japan. eitaira@iwate-med.ac.jp
Kohama
Keiko
K
Tsukamoto
Yasuhiro
Y
Okumura
Shigeki
S
Miki
Naomasa
N
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000502:physiology*; D051929:CD146 Antigen; D002448:Cell Adhesion / Q000502:physiology; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
D015703:Antigens, CD; D051929:CD146 Antigen; D007141:Immunoglobulin Fc Fragments; D009414:Nerve Growth Factors; D019006:Neural Cell Adhesion Molecules; D011993:Recombinant Fusion Proteins