In vitro cell response to a polymer surface micropatterned by laser interference lithography.
This presentation will introduce laser interference lithography to prepare a periodic line and point micropatterns for study of cell-surface interactions. This process provides a straightforward micropatterning technique based on selective laser ablation of polymers utilizing the periodic energy distribution of two or more beam interference patterns. The micropatterns were characterized by atomic force microscopy, while the surface chemical modification was analyzed using X-ray photoelectron spectroscopy. Human pulmonary fibroblasts cultured on the surface of polycarbonate bearing line micropatterns were elongated, spindlelike, and oriented themselves along the line patterns with all different groove widths. In contrast, cells cultured on point patterns were also bipolar but showed no orientation. Further investigations demonstrated that human pulmonary fibroblast cells cultured on line and point micropatterns showed inflammatory response.
Yu
Fayou
F
Department of Materials Science and Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, D-66123 Saarbrücken, Germany. fayou.yu@hella.de
Mücklich
Frank
F
Li
Ping
P
Shen
Hao
H
Mathur
Sanjay
S
Lehr
Claus-Michael
CM
Bakowsky
Udo
U
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007834:Lasers; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D011108:Polymers