title: In vitro cytotoxicity of selected chemicals commonly produced during fire combustion using human cell lines.
abstract: Fire combustion products contain a broad range of chemicals, which have a multitude of possible toxic interactions in humans. The aim of this study was to evaluate the cytotoxicity of selected substances commonly produced during fire combustion. A range of human cell lines and cultures including: skin fibroblasts, HepG2 (liver derived), and A549 (lung derived cells) were used to represent different human target organs. The colorimetric MTS assay (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was used to detect the cytotoxic effects of selected substances including: acetic acid, ammonia, formaldehyde, hydrobromic acid, hydrochloric acid, hydrofluoric acid, potassium cyanide, sodium fluoride, sodium nitrite, sodium sulphide, and sulphurous acid. In this study, the NOAEC (No Observable Adverse Effect Concentration), IC(10) (10% inhibitory concentration), IC50 (50% inhibitory concentration), and TLC (Total Lethal Concentration) values were determined. The ratio between in vitro IC50 to in vivo human toxicity data (Lowest Lethal Dose-LDLo and Lowest Lethal Concentration--LCLo) was also established. Results indicated a strong relationship between IC50 values on the cell types used: fibroblast and A549 (R2: 0.92), A549 and HepG2 (R2: 0.72), fibroblast and HepG2 (R2: 0.69). Good correlation was obtained between the IC50 against LDLo and LCLo when an appropriate adjustment factor was implemented. Results of this study indicated that in vitro methods could be a potential technique for assessing the toxicity of fire combustion products.
authors.affiliation: Chemical Safety and Applied Toxicology (CSAT) Laboratories, School of Safety Science, The University of New South Wales, Sydney 2052, Australia. fatma@student.unsw.edu.au
authors.forename: F
authors.initials: F
authors.lastname: Lestari
authors.forename: A J
authors.initials: AJ
authors.lastname: Hayes
authors.forename: A R
authors.initials: AR
authors.lastname: Green
authors.forename: B
authors.initials: B
authors.lastname: Markovic
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000641:Ammonia / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005390:Fires; D005557:Formaldehyde / Q000633:toxicity; D006801:Humans; D018054:Hydrobromic Acid / Q000633:toxicity; D006858:Hydrofluoric Acid / Q000633:toxicity; D011190:Potassium Cyanide / Q000633:toxicity
chemical_list: D005557:Formaldehyde; D018054:Hydrobromic Acid; D000641:Ammonia; D011190:Potassium Cyanide; D019342:Acetic Acid; D006858:Hydrofluoric Acid