Isolation of a heat-resistant allergen from the fish parasite Anisakis simplex.
The thermal stability of allergenic peptides from the fish parasite Anisakis simplex has not been fully elucidated. This is of special relevance for physicians who should clearly indicate if sensitized patients should avoid ingestion of raw fish only or whether well-cooked fish should also be avoided, if allergenic peptides derived from the parasite remain immunologically detectable. An allergen was purified after heating a crude parasite extract for 30 min. The allergen was further purified by an ethanol fractionation procedure followed by a reversed-phase HPLC. The N-terminal amino acid sequence was obtained. This allergen was detected by 27% of sensitized subjects. The N-terminal amino acid sequence of the 9 kDa allergen showed no similarities to other known proteins. A minor low molecular weight allergen from A. simplex is highly resistant to heating and it could therefore have significant clinical relevance.
Moneo
Ignacio
I
Department of Immunology, Hospital Carlos III, Sinesio Delgado 10, 28029 Madrid, Spain. imoneog@meditex.es
Caballero
Maria Luisa
ML
González-Muñoz
Miguel
M
Rodríguez-Mahillo
Ana I
AI
Rodríguez-Perez
Rosa
R
Silva
Augusto
A
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005399:Fishes / Q000469:parasitology*; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight
D000485:Allergens; D000947:Antigens, Helminth; D007073:Immunoglobulin E; D000431:Ethanol