Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis).
A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium. Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 degrees C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 mug ml(-1)), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15(T), has been deposited in the American Type Culture Collection as ATCC BAA-883(T) and the National Collection of Type Cultures (UK) as NCTC 13318(T).
Rhodes
Martha W
MW
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
Kator
Howard
H
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
McNabb
Alan
A
Laboratory Services, British Columbia Centre for Disease Control, Vancouver BC, Canada V5Z 4R4.
Deshayes
Caroline
C
Avenir Group, Inserm U570, Avenir Group, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France.
Reyrat
Jean-Marc
JM
Avenir Group, Inserm U570, Avenir Group, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France.
Brown-Elliott
Barbara A
BA
Mycobacteria/Nocardia Laboratory, University of Texas Health Center, Tyler, TX 75708, USA.
Wallace
Richard
R
Mycobacteria/Nocardia Laboratory, University of Texas Health Center, Tyler, TX 75708, USA.
Trott
Kristin A
KA
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Parker
John M
JM
Office of Laboratory Animal Care, University of California, Berkeley, CA 94720, USA.
Lifland
Barry
B
Department of Comparative Medicine, Stanford University, Stanford, CA 94305, USA.
Osterhout
Gerard
G
MIDI, Inc. Research and Development, Newark, DE 19713, USA.
Kaattari
Ilsa
I
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
Reece
Kimberly
K
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
Vogelbein
Wolfgang
W
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
Ottinger
Christopher A
CA
US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV 25430, USA.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001492:Bass / Q000382:microbiology*; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D009171:Mycolic Acids / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014768:Virginia
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009171:Mycolic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins