title: Pseudorabies virus glycoprotein gD contains a functional endocytosis motif that acts in concert with an endocytosis motif in gB to drive internalization of antibody-antigen complexes from the surface of infected monocytes.
abstract: Viral glycoproteins gB and gD of the swine alphaherpesvirus pseudorabies virus (PRV), which is closely related to human herpes simplex virus and varicella-zoster virus, are able to drive internalization of antibody-antigen complexes that may form at the cell surface of infected monocytes, thereby protecting these cells from efficient antibody-mediated lysis. We found earlier that gB relies on an endocytosis motif in its cytoplasmic domain for its function during this internalization process. Here, we report that the PRV gD protein also contains a functional endocytosis motif (YRLL) in its cytoplasmic domain that drives spontaneous endocytosis of gD from the cell surface early in infection and that acts in concert with the endocytosis motif in gB to contribute to efficient internalization of antibody-antigen complexes in PRV-infected monocytes.
authors.affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Ficinska
authors.forename: Geert
authors.initials: G
authors.lastname: Van Minnebruggen
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
authors.forename: Krystyna
authors.initials: K
authors.lastname: Bienkowska-Szewczyk
authors.forename: Herman W
authors.initials: HW
authors.lastname: Favoreel
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D000956:Antigens, Viral / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004705:Endocytosis / Q000502:physiology*; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000472:pathogenicity* / Q000502:physiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology* / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D013552:Swine; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000502:physiology
chemical_list: D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D004279:DNA, Viral; D021382:Protein Sorting Signals; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins