Large-scale imaging of cortical network activity with calcium indicators.
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope.
Ikegaya
Yuji
Y
Department of Biological Sciences, Columbia University, 1002 Fairchild Center, M.C. 2435 New York, NY 10027, USA. ikegaya@tk.air.jp
Le Bon-Jego
Morgane
M
Yuste
Rafael
R
D000818:Animals; D002118:Calcium; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
D005456:Fluorescent Dyes; D002118:Calcium