Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3.
The Anc1 protein co-purifies with general transcription factors, chromatin remodeling complexes, and histone modification enzymes and is required for efficient transcription in yeast. We show here that Anc1 interacts with Tsm1, Tfg1, Sth1, Ino80, and Sas3 that are, respectively, the catalytic subunits of the general transcription factors TFIID and TFIIF, of the chromatin remodeling complexes RSC and INO80, and of the histone H3-acetyltransferase complex NuA3. We show that Anc1 is required for growth on galactose as the sole carbon source, and that it is recruited to the UAS of the GAL1 gene after induction.
Kabani
Mehdi
M
Laboratoire de Physiogénomique, Service de Biochimie et Génétique Moléculaire, Departement de Biologie Joliot Curie, Bâtiment 144, CEA/Saclay, F-91191 Gif-sur-Yvette, France. kabani@grignon.inra.fr
Michot
Katia
K
Boschiero
Claire
C
Werner
Michel
M
D000123:Acetyltransferases / Q000378:metabolism*; D001665:Binding Sites; D002384:Catalysis; D018797:Cell Cycle Proteins / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D051548:Histone Acetyltransferases; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C483404:INO80 complex, S cerevisiae; C103337:RSC complex, S cerevisiae; C115017:SPT15 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C090313:TAF14 protein, S cerevisiae; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; C056604:transcription factor TFIIF; D005690:Galactose