Protein sequence tags: a novel solution for comparative proteomics.
Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmaceutically relevant membrane and nuclear proteins. This procedure addresses a similar number of proteins, compared to the multidimensional protein identification technology approach, but offers additionally a quantitative analysis with its recently developed quantitative PST version.
Kuhn
Karsten
K
Proteome Sciences R&D, Frankfurt am Main, Germany.
Prinz
Thorsten
T
Schäfer
Jürgen
J
Baumann
Christian
C
Schärfke
Marco
M
Kienle
Stefan
S
Schwarz
Josef
J
Steiner
Sandra
S
Hamon
Christian
C
D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes; D005656:Fungal Proteins / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D002247:Carbon Isotopes; D005656:Fungal Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010455:Peptides; D020543:Proteome