pIH01, a small cryptic plasmid from Leuconostoc citreum IH3.
A small cryptic plasmid pIH01 from Leuconostoc citreum IH3 was characterized. This 1.8-kb sized plasmid contains single open reading frame that encodes a RepC class protein (342 amino acids) and a conserved pT181-type double strand origin, suggesting a rolling circle replication mode. This putative replicase protein shows the highest similarity to a replicase from pFR18 plasmid of Leuconostoc mesenteroides FR52 (64% identity), one of the pT181-type rolling circle plasmid family and contains a strictly conserved RepC-type active site sequence of pT181 family. A shuttle vector that was developed on the basis of this cryptic plasmid by insertion of both erythromycin resistance gene (ermC) from pE194 and Escherichia coli ColE1 origin was able to transform Leuconostoc strains, Lactobacillus plantarum, and Lactococcus lactis. Therefore, pIH01 derivative plasmids might be useful for the manipulation of Leuconostoc strains.
Park
Junghyun
J
Department of Biological Sciences, Inha University, Inchon 402-751, Republic of Korea.
Lee
Mihye
M
Jung
Joohwa
J
Kim
Jeongho
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017124:Conserved Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D005822:Genetic Vectors; D048191:Lactiplantibacillus plantarum / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics; D007934:Leuconostoc / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; C043650:RepC protein, Staphylococcus aureus; D004917:Erythromycin