title: Adenovirus type 7 peptide diversity during outbreak, Korea, 1995-2000.
abstract: To understand the molecular basis of observed regional shifts in the genome types of adenovirus type 7 (Ad7) isolated in Korea during nationwide outbreaks from 1995 to 2000, the genetic variabilities of Ad7d and Ad7l were studied by sequence analysis of hexon, fiber, E3, and E4 open reading frame (ORF) 6/7 peptides. One amino acid change in the receptor-binding domain of fiber and 6 amino acid variations in E4 ORF 6/7 were identified between 2 genome types, while no variations were found in hexon and E3. Phylogenetic trees based on hexon, fiber, and E4 suggested that the Ad7 epidemic was probably caused by the introduction of the Japanese Ad7d strains. Our data also provide evidence that the rapid divergence of Ad7d to a novel genome type Ad7l could have been due to viral strategies involving multiple sequence changes in E4. This result suggests fiber and E4 ORF 6/7 peptides participate in the evolution of Ad7.
authors.affiliation: Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eun Hwa
authors.initials: EH
authors.lastname: Choi
authors.forename: Hee Sup
authors.initials: HS
authors.lastname: Kim
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Eun
authors.forename: Beyong Il
authors.initials: BI
authors.lastname: Kim
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Choi
authors.forename: Hoan Jong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Toshiki
authors.initials: T
authors.lastname: Inada
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics*; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D015183:Restriction Mapping