D-lactate metabolism in starved Octopus ocellatus.
The concentrations of D- and L-lactate, methylglyoxal and pyruvate were measured in tissues of normal and starved Octopus ocellatus. D-Lactate was always more abundant than L-lactate in the tissues. D-Lactate, pyruvate and methylglyoxal were present in 320, 94 and 43 times higher concentrations in tentacle of O. ocellatus of control group than those in normal rat skeletal muscle. The D-lactate concentration in the tentacle of O. ocellatus was 17-fold higher than that in Octopus vulgars. The activities of enzymes involved with D-lactate metabolism such as pyruvate kinase, octopine dehydrogenase, glyoxalase I and II and lactate dehydrogenase were measured in those tissues. The activities of glyoxalase I and II, and D-lactate dehydrogenase were increased in mantle and tentacle of starved octopus, while the levels of D-lactate and related metabolites were lowered in these tissues. The experimental results presented in this report and up to the present indicate that D-lactate is actively used for energy production in the tentacle and mantle of the starved animals. In octopus, especially starved octopus D-lactate was actively produced from methylglyoxal, which is formed via aminoacetone from threonine and glycine.
Fujisawa
Tomomi
T
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 7008530, Japan.
Akagi
Shinsuke
S
Kawase
Michi
M
Yamamoto
Masamichi
M
Ohmori
Shinji
S
D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D004798:Enzymes / Q000378:metabolism; D005121:Extremities / Q000502:physiology; D005508:Food Deprivation / Q000502:physiology*; D007564:Japan; D019344:Lactic Acid / Q000378:metabolism*; D049831:Octopodiformes / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D013053:Spectrophotometry
D004798:Enzymes; D019344:Lactic Acid; D011765:Pyruvaldehyde; D019289:Pyruvic Acid