title: Variations of dominant microbial populations in groundwater in response to the leachate from Laogang Landfill.
abstract: Temporal changes of dominant microbial populations in groundwater in response to the leachate from Shanghai Laogang Landfill were investigated. Concentrations of dissolved redox-relevant species in groundwater suggested that the dominating redox process had changed from denitrification to methane-production/sulfate-reduction due to landfilling. Dominant microbial populations were determined using restriction fragment length polymorphism( RFLP) analyses of 16S rRNA gene libraries, which were further studied by sequencing and phylogenetic analyses. The results indicated that obvious shifts of dominant microbial populations had occurred in groundwater in response to the pollution of leachate. The closest relatives of some dominant clones are accordant with the dominating redox processes determined by hydrochemical analyses, based on the GenBank's indications on the ability to perform redox reactions.
authors.affiliation: School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Yang-jie
authors.initials: YJ
authors.lastname: Tian
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Dao-tang
authors.initials: DT
authors.lastname: Li
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Lin
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D002681:China; D017753:Ecosystem; D005618:Fresh Water / Q000382:microbiology*; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical