title: Expression of P2X4 receptor by lesional activated microglia during formalin-induced inflammatory pain.
abstract: P2X4 receptor (P2X4R) is an ion channel gated by adenosine 5'-triphosphate. Here we report the presence and the distribution of P2X4R in rat spinal cord by immunohistochemical analysis in an inflammatory pain model. Peripheral inflammation was induced by subcutaneous injection of 4% formalin into the rat hindpaw. Morphology, spatial localization, and activation state of P2X4R+ cells were described at 1, 5, 7, 14, and 28 days after injury. In normal and saline treated control rats, P2X4R was rarely seen. After formalin administration, an increase of P2X4R+ microglia were observed in the spinal cord dorsal horn on the side ipsilateral to the injection, reaching maximal levels by day 7, and then decreasing to normal levels by day 14. This implicates a role of P2X4R in the spinal inflammatory pain process. Furthermore, formalin-induced region-specific increase in activated microglia was confirmed by ED1 and endothelial monocytes activating polypeptide II (EMAP-II) expression. In conclusion, this is the first demonstration that P2X4R is expressed by microglia in the inflammatory pain.
authors.affiliation: Institute of Brain Research, University of Tuebingen, Calwer Str.3, D-72076 Tuebingen, Germany. lianghao.guo@uni-tuebingen.de
authors.forename: Liang-Hao
authors.initials: LH
authors.lastname: Guo
authors.forename: Katrin
authors.initials: K
authors.lastname: Trautmann
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Schluesener
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D008161:Lumbosacral Region; D008297:Male; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D010146:Pain / Q000378:metabolism* / Q000473:pathology; D010147:Pain Measurement / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis* / Q000235:genetics; D058848:Receptors, Purinergic P2X4; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology
chemical_list: C548228:P2rx4 protein, rat; D018048:Receptors, Purinergic P2; D058848:Receptors, Purinergic P2X4