Quorum-sensing-based toolbox for regulatable transgene and siRNA expression in mammalian cells.
Technologies for regulated expression of multiple transgenes in mammalian cells have gathered momentum for bioengineering, gene therapy, drug discovery, and gene-function analyses. Capitalizing on recently developed mammalian transgene modalities (QuoRex) derived from Streptomyces coelicolor, we have designed a flexible and highly compatible expression vector set that enables desired transgene/siRNA control in response to the nontoxic butyrolactone SCB1. The construction-kit-like expression portfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional (pBiRex), (iv) oncoretro- and lentiviral transduction, and (v) RNA polymerase II-based siRNA transcription-fine-tuning vectors for straightforward implementation of QuoRex-controlled (trans)gene modulation in mammalian cells.
Weber
Wilfried
W
Institute of Chemical- and Bio-Engineering, Swiss Federal Institute of Technology, ETH Hoenggerberg HCI F115, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Malphettes
Laetitia
L
Rinderknecht
Matthias
M
Schoenmakers
Ronald G
RG
Spielmann
Manuela
M
Keller
Bettina
B
van de Wetering
Petra
P
Weber
Cornelia C
CC
Fussenegger
Martin
M
D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods; D006224:Cricetinae; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology*
D034741:RNA, Small Interfering