title: Development of a monoclonal antibody against Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) that can detect EBNA-LP expressed in P3HR1 cells.
abstract: A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody was mapped in the W2 domain of EBNA-LP. While another monoclonal antibody to EBNA-LP, JF186, which is widely used for analyses of the viral protein, did not react with truncated forms of EBNA-LP expressed in P3HR1 cells, as reported earlier, the LP4D3 antibody did. The LP4D3 antibody will be a useful tool for further studies of EBNA-LP, especially investigations into the phenotypes of mutant EBNA-LP expressed in P3HR1 cells.
authors.affiliation: Department of Cell Regulation, Medical Research Institute, Tokyo, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shaku
authors.forename: Go
authors.initials: G
authors.lastname: Matsuda
authors.forename: Rie
authors.initials: R
authors.lastname: Furuya
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Kamagata
authors.forename: Mie
authors.initials: M
authors.lastname: Igarashi
authors.forename: Michiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kanamori
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawaguchi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D018604:Epitope Mapping; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C402640:EBNA-LP protein, Human herpesvirus 4; D014764:Viral Proteins