Histoculture drug response assay to monitor chemoresponse.
We provide a detailed explanation of the procedure of the histoculture drug response assay (HDRA) with 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) end point among several modified HDRA procedures. Fresh surgical specimens are cut into approx 1- to 2-mm3 pieces and put on a gelatin sponge infiltrated with culture medium containing a test drug. After incubation for 7 d, cell viability is assessed by the MTT assay. HDRA uses cancer tissue fragments with cells growing in three dimensions, with maintenance of intercellular contact and interactions with stromal cells. Therefore, it seems that HDRA can assess the sensitivity of tumor cells to anticancer drugs in conditions similar to those in vivo and, consequently, shows high prediction rate.
Ohie
Shinji
S
Cancer Research Laboratory, Hanno Research Center, Taiho Pharmaceutical Co., Saitama, Japan.
Udagawa
Yasuhiro
Y
Aoki
Daisuke
D
Nozawa
Shiro
S
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001706:Biopsy; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005780:Gelatin; D005782:Gels; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology; D013778:Tetrazolium Salts; D013844:Thiazoles; D046509:Tissue Culture Techniques
D000970:Antineoplastic Agents; D004396:Coloring Agents; D005782:Gels; D013778:Tetrazolium Salts; D013844:Thiazoles; D005780:Gelatin; C022616:thiazolyl blue