title: Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis).
abstract: A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium. Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 degrees C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 mug ml(-1)), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15(T), has been deposited in the American Type Culture Collection as ATCC BAA-883(T) and the National Collection of Type Cultures (UK) as NCTC 13318(T).
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: Martha W
authors.initials: MW
authors.lastname: Rhodes
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: Howard
authors.initials: H
authors.lastname: Kator
authors.affiliation: Laboratory Services, British Columbia Centre for Disease Control, Vancouver BC, Canada V5Z 4R4.
authors.forename: Alan
authors.initials: A
authors.lastname: McNabb
authors.affiliation: Avenir Group, Inserm U570, Avenir Group, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Deshayes
authors.affiliation: Avenir Group, Inserm U570, Avenir Group, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Reyrat
authors.affiliation: Mycobacteria/Nocardia Laboratory, University of Texas Health Center, Tyler, TX 75708, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Brown-Elliott
authors.affiliation: Mycobacteria/Nocardia Laboratory, University of Texas Health Center, Tyler, TX 75708, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Wallace
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Trott
authors.affiliation: Office of Laboratory Animal Care, University of California, Berkeley, CA 94720, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Parker
authors.affiliation: Department of Comparative Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: Barry
authors.initials: B
authors.lastname: Lifland
authors.affiliation: MIDI, Inc. Research and Development, Newark, DE 19713, USA.
authors.forename: Gerard
authors.initials: G
authors.lastname: Osterhout
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: Ilsa
authors.initials: I
authors.lastname: Kaattari
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Reece
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Vogelbein
authors.affiliation: US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV 25430, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Ottinger
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001492:Bass / Q000382:microbiology*; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D009171:Mycolic Acids / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014768:Virginia
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009171:Mycolic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins