title: Mycobacterium florentinum sp. nov., isolated from humans.
abstract: Eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were Mycobacterium triplex and Mycobacterium lentiflavum. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of hsp65. The strains presented high resistance to antimycobacterial drugs. The name Mycobacterium florentinum sp. nov. is proposed for this taxon, with strain FI-93171(T) (=DSM 44852(T) = CIP 108409(T)) as the type strain.
authors.affiliation: Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
authors.affiliation: Department of Experimental Pathology, Medical Biotechnologies, Infectivology and Epidemiology, University of Pisa, 56127 Pisa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Rindi
authors.affiliation: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, 97165 Pointe-à-Pitre, Guadeloupe, France.
authors.forename: Khye S
authors.initials: KS
authors.lastname: Goh
authors.affiliation: Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland.
authors.forename: Marja L
authors.initials: ML
authors.lastname: Katila
authors.affiliation: Genetics and Cytogenetics Unit, Careggi Hospital, 50134 Florence, Italy.|Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mariottini
authors.affiliation: Clinical Laboratory, Campo di Marte Hospital, 55100 Lucca, Italy.
authors.forename: Romano
authors.initials: R
authors.lastname: Mattei
authors.affiliation: Microbiological and Virological Serum-immunology Laboratory, Careggi Hospital, 50134 Florence, Italy.|Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Gianna
authors.initials: G
authors.lastname: Mazzarelli
authors.affiliation: Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland.
authors.forename: Sini
authors.initials: S
authors.lastname: Suomalainen
authors.affiliation: Laboratory of Environmental Microbiology, National Public Health Institute, 70211 Kuopio, Finland.|Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland.
authors.forename: Pirjo
authors.initials: P
authors.lastname: Torkko
authors.affiliation: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, 97165 Pointe-à-Pitre, Guadeloupe, France.
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D002648:Child; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D008198:Lymph Nodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins