Establishment of a novel human B-CLL-like xenograft model in nude mouse.
We have developed a novel murine model for B-CLL by engrafting human prolymphocytic leukemia (PLL) or B-CLL cell line cells (JVM-3 and MEC-2 cell lines, respectively) into nude mice. Not only treatment of the mice was a prerequisite for the success of the graft, but also for the first time, females appeared to accept the cells more easily than males. Surprisingly, tumoral murine models for B-CLL could be established with PLL cells but not with B-CLL cells. JVM-3 cells were efficiently transplanted into nude mice through subcutaneous or intravenous routes. Irradiated female mice appeared to be the optimal recipients for tumor growth. Such murine models for human B-CLL may help the development of therapeutic agents.
Loisel
Severine
S
Laboratory of Cellular Therapy and Cancer Immunobiology, Brest University Medical School Hospital, BP824, F29609 Brest Cedex, France. severine.loisel@univ-brest.fr
Ster
Karine Le
KL
Quintin-Roue
Isabelle
I
Pers
Jacques-Olivier
JO
Bordron
Anne
A
Youinou
Pierre
P
Berthou
Christian
C
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012737:Sex Factors; D015996:Survival Rate; D014183:Transplantation, Heterologous; D023041:Xenograft Model Antitumor Assays / Q000379:methods*
D014408:Biomarkers, Tumor