title: Expression of Xenopus suppressor of cytokine signaling 3 (xSOCS3) is induced by epithelial wounding.
abstract: The suppressor of cytokine signaling (SOCS) family of proteins are intracellular mediators of cytokine signaling. These proteins are induced rapidly by cytokine stimulation and act in a classic negative-feedback loop to attenuate the cellular response to the cytokine signal. In this study, we present the cloning and initial characterization of the Xenopus SOCS3 gene. We show that xSOCS3 is rapidly induced in response to epithelial wounding in the tadpole. The induction of xSOCS3 in response to trauma is transient with maximal expression being reached 1 hr after the injury and diminishing after that. Unlike other genes known to be responsive to wound-induced activation of the mitogen-activated protein (MAP) kinase pathway, such as Egr1, SOCS3 expression in response to trauma is unaffected by blockade of the MAP kinase pathway by chemical inhibitors.
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
authors.forename: Emin
authors.initials: E
authors.lastname: Kuliyev
authors.forename: Joanne R
authors.initials: JR
authors.lastname: Doherty
authors.forename: Paul E
authors.initials: PE
authors.lastname: Mead
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D047108:Embryonic Development / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D004848:Epithelium / Q000293:injuries / Q000378:metabolism* / Q000473:pathology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D000071223:Suppressor of Cytokine Signaling 3 Protein; D014947:Wounds and Injuries / Q000235:genetics* / Q000473:pathology*; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000293:injuries
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D047428:Protein Kinase Inhibitors; D012097:Repressor Proteins; C504914:SOCS3 protein, Xenopus; D000071223:Suppressor of Cytokine Signaling 3 Protein; D029867:Xenopus Proteins; D048049:Extracellular Signal-Regulated MAP Kinases