[In vitro antitumor effect of adenovirus containing CD-TK fusion gene driven by VEGF promoter on LoVo cells].
OBJECTIVE
To evaluate the in vitro antitumor effect of adenovirus containing cytosine deaminase (CD) and thymine kinase (TK) gene driven by vascular endothelial growth factor (VEGF) promoter (Ad-VEGFp-CDglyTK) on human colorectal cancer LoVo cells.


METHODS
The adenoviral vectors Ad-VEGFp-CDglyTK, Ad-CMV-CD, Ad-CMV-TK and Ad-CMV- CDglyTK were transfected into LoVo cells, which were then treated with the prodrugs 5-FC or GCV to observe the plating efficiency and growth inhibition effect and bystander effect of the vectors on LoVo cells.


RESULTS
Compared with the two vectors containing single suicide gene, Ad-VEGFp-CDglyTK showed more remarkable inhibitory effect on the plating efficiency and growth of the tumor cells, and the bystander effect of the fusion suicide gene was much stronger than that produced by single suicide gene. But the LoVo cells infected with Ad-VEGFp-CDglyTK exhibited similar sensitivity to the prodrugs with the cells infected with Ad-CMV-CDglyTK.


CONCLUSIONS
Ad-VEGFp-CDglyTK has potent antitumor effect on LoVo cells in vitro, which is comparable with the effect of Ad-CMV-CDglyTK.
Huang
Zong-hai
ZH
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Chen
Jian-xiong
JX
Su
Guo-qiang
GQ
D000256:Adenoviridae / Q000235:genetics; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000628:therapy; D043525:Cytosine Deaminase / Q000235:genetics*; D041722:Genes, Transgenic, Suicide / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D013937:Thymidine Kinase; D043525:Cytosine Deaminase