An HPLC-PAD method to analyse flavonoid glycosides and styrylpyrones from Cryptocarya species (Lauraceae).
An HPLC-PAD method has been developed in order to evaluate simultaneously the main secondary metabolites, flavonoid glycosides and styrylpyrones, of leaves of Cryptocarya moschata. The sample preparation, consisting of extraction, liquid-liquid extraction and centrifugation, requires minimum sample manipulation but produces high yields with reproducibility, selectivity and simplicity. HPLC on a C18 column presents each class of metabolites grouped and with good resolution of the main compounds. The experimental conditions can be used to study inter- and intra-specific variability of secondary metabolites in Cryptocarya spp.
Nehme
Cláudia J
CJ
Núcleo de Bioensaios, Bissíntese e Ecofisiologia de Produtos Naturais, Instituto de Química, UNESP, Araraquara-SP 14800-900, Brazil.
Bastos
Wagner L
WL
de Araújo
Adelson J
AJ
Cavalheiro
Alberto J
AJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031369:Cryptocarya / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D015394:Molecular Structure; D011753:Pyrones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005419:Flavonoids; D006027:Glycosides; D011753:Pyrones