Leuconostoc durionis sp. nov., a heterofermenter with no detectable gas production from glucose.
Three lactic acid bacterial (LAB) strains obtained from a Malaysian acid-fermented condiment, tempoyak (made from pulp of the durian fruit), showed analogous but distinct patterns after screening by SDS-PAGE of whole-cell proteins and comparison with profiles of all recognized LAB species. 16S rRNA gene sequencing of one representative strain showed that the taxon belongs phylogenetically to the genus Leuconostoc, with its nearest neighbour being Leuconostoc fructosum (98 % sequence similarity). Biochemical characteristics and DNA-DNA hybridization experiments demonstrated that the strains differ from Leuconostoc fructosum and represent a single, novel Leuconostoc species for which the name Leuconostoc durionis sp. nov. is proposed. The type strain is LMG 22556(T) (= LAB 1679(T) = D-24(T) = CCUG 49949(T)).
Leisner
J J
JJ
Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
Vancanneyt
M
M
BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
Van der Meulen
R
R
Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing (IMDO), Department of Applied Biological Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Lefebvre
K
K
BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
Engelbeen
K
K
BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
Hoste
B
B
BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
Laursen
B G
BG
Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
Bay
L
L
Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
Rusul
G
G
Department of Food Science, Universiti Putra Malaysia, Serdang, Selangor D.E., Malaysia.
De Vuyst
L
L
Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing (IMDO), Department of Applied Biological Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Swings
J
J
BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D003212:Condiments / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005947:Glucose / Q000378:metabolism*; D007934:Leuconostoc / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005947:Glucose