title: Ligand-dependent protein interactions of the estrogen receptors using the yeast two-hybrid system.
abstract: The protein-interacting ability of estrogen receptor beta (ERbeta) and estrogen receptor alpha (ERalpha) from Atlantic salmon (Salmo salar) was studied using the yeast two-hybrid system. The salmon ERbeta shared features with other nuclear receptors regarding dimerization, forming a homodimer and heterodimer with ERalpha. The activation of salmon ERs by estradiol-17beta was shown to be necessary for dimerization. Furthermore, the xenoestrogens, 4-nonylphenol and bisphenol A, were shown to promote ER dimerization in the yeast two-hybrid system.
authors.affiliation: Department of Molecular Biology, University of Bergen, Norway.
authors.forename: Bergithe E
authors.initials: BE
authors.lastname: Oftedal
authors.forename: Sigve
authors.initials: S
authors.lastname: Ladstein
authors.forename: Wenche
authors.initials: W
authors.lastname: Telle
authors.forename: Rune
authors.initials: R
authors.lastname: Male
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D008024:Ligands; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D020317:Salmo salar; D020798:Two-Hybrid System Techniques
chemical_list: D008024:Ligands; D011960:Receptors, Estrogen