Identification of non-mutans streptococci organisms in dental plaques recovering on mitis-salivarius bacitracin agar medium.
The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.
Yoo
So Young
SY
Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea.
Kim
Pyung Sik
PS
Hwang
Ho-Keel
HK
Lim
Seong-Hoon
SH
Kim
Kwang-Won
KW
Choe
Son-Jin
SJ
Min
Byung-Moo
BM
Kook
Joong-Ki
JK
D000293:Adolescent; D000328:Adult; D000362:Agar; D001414:Bacitracin; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D013291:Streptococcus / Q000302:isolation & purification*
D003470:Culture Media; D001414:Bacitracin; D000362:Agar