Identification of a glucokinase that generates a major glucose phosphorylation activity in the cyanobacterium Synechocystis sp. PCC 6803.
In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.
Lee
Jung-Mi
JM
Department of Biology, Chungnam National University, Daejeon 305-764, Korea.
Ryu
Jee-Youn
JY
Kim
Hyong-Ha
HH
Choi
Sang-Bong
SB
de Marsac
Nicole Tandeau
NT
Park
Youn-Il
YI
D000595:Amino Acid Sequence; D049109:Cell Proliferation; D000458:Cyanobacteria / Q000201:enzymology*; D016680:Genome, Bacterial; D005941:Glucokinase / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D012329:RNA, Bacterial; D009249:NADP; D005941:Glucokinase; D005947:Glucose; D010100:Oxygen