title: Frequency and diagnosis of bacterial vaginosis.
abstract: OBJECTIVE
  To determine the frequency of bacterial vaginosis (BV) in women with vaginal discharge, and to compare different diagnostic tests for its diagnosis.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  The Aga Khan University Hospital from June 1998 to May 2000.
  
  
  METHODS
  All women attending the Obstetrics and Gynecology clinics at The Aga Khan University Hospital, with the complaints of vaginal discharge, were examined with the help of a speculum. The vaginal pH was measured, samples for bacterial cultures were obtained. A slide was prepared for the gram's stain and Whiff-test was also performed. BV was diagnosed, when the vaginal discharge fulfilled at least three of the composite clinical criteria (Amsel's criteria), a standard method for the diagnosis of bacterial vaginosis: homogenous (pasty) discharge, pH more than 4.5, positive Whiff-test and the presence of clue cells.
  
  
  RESULTS
  The frequency of BV was observed to be 16.1% . The culture for Gardnerella vaginalis was compared with the composite clinical criteria. The sensitivity, specificity, positive and negative predictive values of the culture, calculated for the diagnosis of BV were 93.8%, 70%, 37.7% and 98% respectively.
  
  
  CONCLUSIONS
  The use of laboratory tests in conjunction with clinical findings is necessary for diagnosis of BV. The composite clinical criteria for the diagnosis of BV are rapid, reliable and inexpensive method.
authors.affiliation: Department of Obstetrics & Gynaecology, The Aga Khan University Hospital, Karachi, Pakistan. yasminluni@hotmail.com
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Luni
authors.forename: Shama
authors.initials: S
authors.lastname: Munim
authors.forename: Rahat
authors.initials: R
authors.lastname: Qureshi
authors.forename: Abdul Latif
authors.initials: AL
authors.lastname: Tareen
mesh_terms: D000293:Adolescent; D000328:Adult; D003430:Cross-Sectional Studies; D003944:Diagnostic Techniques, Obstetrical and Gynecological; D005260:Female; D006785:Hospitals, University; D006801:Humans; D008875:Middle Aged; D010154:Pakistan; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D019522:Vaginal Discharge / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000150:complications / Q000175:diagnosis*