Redox-responsive and calcium-dependent switching of glycosyldisulfide interactions with Concanavalin A.
Glycosyldisulfides can interact efficiently with carbohydrate-binding entities. This has been shown for a range of thiosaccharide dimers when tested for their effects against the lectin Concanavalin A using a modified quartz crystal microbalance-technique. Contrary to the thiosaccharide monomers, showing no significant binding up to 10 mM, several of the dimers showed IC(50)-values in the low millimolar range. Three of the glycosyldisulfides tested also displayed very high positive apparent cooperativity effects that were found to be both calcium-dependent and redox-responsive.
Pei
Zhichao
Z
KTH-Royal Institute of Technology, Department of Chemistry, Stockholm, Sweden.
Aastrup
Teodor
T
Anderson
Henrik
H
Ramström
Olof
O
D002118:Calcium / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003208:Concanavalin A / Q000737:chemistry*; D019281:Dimerization; D006027:Glycosides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013440:Sulfides / Q000737:chemistry*
D006027:Glycosides; D013440:Sulfides; D003208:Concanavalin A; D002118:Calcium