title: Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil.
abstract: A Gram-positive, aerobic, non-motile, mesophilic strain, designated YIM 70056(T), was isolated from a forest soil sample in Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had less than 97.0 % similarity to any Rhodococcus species with validly published names, with the exception of Rhodococcus fascians (DSM 20669(T)), which was found to be its closest neighbour (98.9 % similarity). Chemotaxonomic data, including peptidoglycan type, diagnostic sugar compositions, fatty acid profiles, menaquinones, polar lipids and mycolic acids, were determined for this isolate; the results supported the affiliation of strain YIM 70056(T) to the genus Rhodococcus. The DNA G + C content was 63.5 mol%. The results of DNA-DNA hybridization with R. fascians DSM 20669(T), in combination with chemotaxonomic and physiological data, demonstrated that isolate YIM 70056(T) represents a novel Rhodococcus species, for which the name Rhodococcus yunnanensis sp. nov. is proposed, with YIM 70056(T) (=CCTCC AA 204007(T) = KCTC 19021(T) = DSM 44837(T)) as the type strain.
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Guo-Zhong
authors.initials: GZ
authors.lastname: Chen
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D014197:Trees; D024482:Vitamin K 2 / Q000032:analysis / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010457:Peptidoglycan; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D005840:Gentian Violet