title: [Yersinia pseudotuberculosis nucleoside-kinase].
abstract: Enzyme capable of catalyzing the phosphorylation of thymidine and uridine was isolated from Y. pseudotuberculosis cells by fractionation with the use of ammonium sulfate, ion exchange and affinity chromatography. The degree of purification of thymidine- and uridine-kinase was approximately 350 times, and at all stages of isolation the activity of both nucleoside-kinases was detected in the same peaks. The purified enzyme was capable of the phosphorylation of thymidine and uridine at temperatures of 8-10 degrees C to 50 degrees C and exhibited the maximum enzymatic activity at pH 8-8.5 and 45 degrees C in the presence of 0.5-1.0 mM MgCl2 and 2 mM ATP. The enzyme was found to have no strict substrate specificity and transferred the phosphate group from ATP to radiolabeled thymidine, uridine and desoxycytidine with different effectiveness, but did not use thymidine-monophosphate as phosphate acceptor.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Nemtseva
authors.forename: N A
authors.initials: NA
authors.lastname: Terent'eva
authors.forename: N F
authors.initials: NF
authors.lastname: Timchenko
authors.forename: L L
authors.initials: LL
authors.lastname: Terent'ev
authors.forename: V A
authors.initials: VA
authors.lastname: Rasskazov
mesh_terms: D000255:Adenosine Triphosphate; D000645:Ammonium Sulfate; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride; D010766:Phosphorylation; D013696:Temperature; D013937:Thymidine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D014541:Uridine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000201:enzymology*
chemical_list: D015636:Magnesium Chloride; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase; D014541:Uridine Kinase; D000645:Ammonium Sulfate