title: Genomic analysis of Hyphomonas neptunium contradicts 16S rRNA gene-based phylogenetic analysis: implications for the taxonomy of the orders 'Rhodobacterales' and Caulobacterales.
abstract: Hyphomonas neptunium is a marine prosthecate alpha-proteobacterium currently classified as a member of the order 'Rhodobacterales'. Although this classification is supported by 16S rRNA gene sequence phylogeny, 23S rRNA gene sequence analysis, concatenated ribosomal proteins, HSP70 and EF-Tu phylogenies all support classifying Hyphomonas neptunium as a member of the Caulobacterales instead. The possible reasons why the 16S rRNA gene sequence gives conflicting results in this case are also discussed.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850, USA.
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Badger
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850, USA.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Eisen
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850, USA.
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Ward
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D018840:HSP70 Heat-Shock Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D010444:Peptide Elongation Factor Tu