Endurance training increases lactate transport in male Zucker fa/fa rats.
The purpose of this study was to investigate the effect of endurance training (10 weeks) on previously reported alterations of lactate exchange in obese Zucker fa/fa rats. We used sarcolemmal vesicles to measure lactate transport capacity in control sedentary rats, Zucker (fa/fa), and endurance trained Zucker (fa/fa) rats. Monocarboxylate transporter (MCT) 1 and 4 content was measured in sarcolemmal vesicles and skeletal muscle. Training increased citrate synthase activity in soleus and in red tibialis anterior, and improved insulin sensitivity measured by intraperitoneal glucose tolerance test. Endurance training increased lactate influx in sarcolemmal vesicles at 1 mM of external lactate concentration and increased MCT1 expression on sarcolemmal vesicles. Furthermore, muscular lactate level was significantly decreased after training in red tibialis anterior and extensor digitorum longus. This study shows that endurance training improves impairment of lactate transport capacity that is found in insulin resistance state like obesity and type 2 diabetes.
Metz
Lore
L
Département de Physiologie des Interactions, EA 701 Muscles et Pathologies chroniques, Institut de biologie, CS 89508, 34060 Montpellier cedex 2, France. lore_metz@hotmail.com
Vermaelen
Marianne
M
Lambert
Karen
K
Broca
Christophe
C
Sirvent
Pascal
P
Raynaud
Eric
E
Mercier
Jacques
J
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D001835:Body Weight; D005951:Glucose Tolerance Test; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal; D051381:Rats; D011924:Rats, Zucker
D019344:Lactic Acid