[Cloning and expression of a gene encoding shortened cecropin A-melittin hybrid in E.coli].
According to the studies of David Andreu and other scientists, we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid. This hybrid consists of CA(1-7) and M(5-12). The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI. A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).
Zhu
Jia-ming
JM
Department of Biotechnology, College of Life Scienceand Technology, Jinan University, Guangzhou 510630, China. tom@jnu.edu.cn
Liu
Fei-peng
FP
Li
Yue-qin
YQ
Zhong
Zhen-yu
ZY
Zhang
Na
N