title: Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43.
abstract: Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a > or =4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.
authors.affiliation: Center of Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, People's Republic of China. linche@pub.guangzhou.gd.cn
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Che
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Qiu
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liao
authors.forename: Ya-di
authors.initials: YD
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Wen
authors.forename: Yu-Xian
authors.initials: YX
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Hao
authors.forename: Ya-Bo
authors.initials: YB
authors.lastname: Mei
authors.forename: Vincent C C
authors.initials: VC
authors.lastname: Cheng
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood*; D028941:Coronavirus 229E, Human / Q000276:immunology*; D028962:Coronavirus OC43, Human / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M