A method of spectral subband decomposition by simultaneous fitting the initial spectrum and a set of its derivatives.
An improved method for spectral subband decomposition based on simultaneous fitting of the initial spectrum and a set of its derivatives is introduced. Additionally, a procedure for finding an optimal smoothing filter to obtain undistorted derivatives is suggested. The proposed method is demonstrated with a model spectrum as well as with experimental absorption spectra of the photosynthetic antenna complexes, peridinin-chlorophyll a-protein (PCP) and the main light-harvesting complex of higher plants (LHC II).
Mikhailyuk
Igor K
IK
A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119992, Russia.
Lokstein
Heiko
H
Razjivin
Andrej P
AP
D000818:Animals; D002338:Carotenoids / Q000737:chemistry*; D002734:Chlorophyll; D004141:Dinoflagellida; D045342:Light-Harvesting Protein Complexes; D015800:Protozoan Proteins / Q000737:chemistry*; D012984:Software; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis / Q000379:methods*
D045342:Light-Harvesting Protein Complexes; D015800:Protozoan Proteins; C073227:peridinin chlorophyll-a protein, Dinophyceae; D002734:Chlorophyll; D002338:Carotenoids