[Primarily study and significance of ET-1 during ischemia with RT-PCR].
OBJECTIVE
To explore the relationship between expression of endothelin in heart and ischemia.


METHODS
Using the RT-PCR method, we enquiry changes of ET-1 RNA after 60min ischemia in hearts of rats.


RESULTS
Although the two group appear positive, they are different significantly, which indicate that ischemia may lead to the increase of ET-1 mRNA.


CONCLUSIONS
The experiment, therefore, pave a way for immunochemical study.
Ma
Meng-yun
MY
Shenzhen Institute of Criminal Science and Technology, Shenzhen 518001, China.
Xu
Xiao-hu
XH
D000818:Animals; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D007091:Image Processing, Computer-Assisted; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019332:Endothelin-1; D012333:RNA, Messenger