title: [Comparision of several different peritoneal fibrosis rat models].
abstract: OBJECTIVE
  To compare different types of peritoneal fibrosis models in rats.
  
  
  METHODS
  Thirty-four SD rats were divided into 5 groups: control group (Group 1), normal saline group (Group 2), high glucose group (4.25% peritoneal dialysate, 4.25% PDF, Group 3), high glucose + lipopolysaceharides (LPS) group (4.25% PDF + LPS, Group 4), high glucose + erythromycin group (4.25% PDF + lactobionate erythromycin, Group 5). A 2-hour peritoneal equilibration test (PET) was performed after 5 weeks. Then animals were humanely killed. Dialysate-to-plasma urea ratio (D/ Purea), glucose reabsorption (D2/D0), net ultrafiltration (UF) volume were determined. The level of fibronectin in peritoneal tissues was measured by immunohistochemical method. Peritoneal membrane histology was evaluated by light microscopy.
  
  
  RESULTS
  The D2/D0 ratio and net ultrafiltration volume in Groups 3, 4, and 5 were significantly lower than those in Groups 1 and 2 (P < 0.05) . The D/Purea ratio in Groups 3, 4 and 5 were significantly higher than that in Groups 1 and 2 (P < 0. 05 ). The level of fibronectin in Groups 3, 4 and 5 were significantly higher than that in Groups 1 and 2 (P < 0.05 ).
  
  
  CONCLUSIONS
  Different types of peritoneal fibrosis models in rats has been established. The best model is high clusion glucose + erythromycin.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital, Changsha 410011, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhuo
authors.forename: Fu-you
authors.initials: FY
authors.lastname: Liu
authors.forename: You-ming
authors.initials: YM
authors.lastname: Peng
authors.forename: Ying-hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Ling-hui
authors.initials: LH
authors.lastname: Zhou
authors.forename: Xiang-qing
authors.initials: XQ
authors.lastname: Xu
authors.forename: Jie-han
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005355:Fibrosis / Q000139:chemically induced / Q000209:etiology; D008297:Male; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D010537:Peritoneum / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley