Single-molecule detection of biomolecules by surface-enhanced coherent anti-Stokes Raman scattering.
We report on the applicability of combining surface-enhanced Raman scattering (SERS) with coherent anti-Stokes Raman scattering for high-sensitivity detection of biological molecules. We found that this combination of techniques provides more than 3 orders of signal enhancement compared with SERS and permits monitoring of biological molecules such as deoxyguanosine monophosphate (dGMP) and deoxyadenosine monophosphate at the single-molecule level. This combined technique also improved detection sensitivity for angiotensin peptide. As this is believed to be the first report of detection of dGMP at the single-molecule level, we suggest that this approach can serve as a new tool for biological studies.
Koo
Tae-Woong
TW
Precision Biology, Intel Research/CTM, Intel Corporation, Santa Clara, California 95054, USA. tae-woong.t.koo@intel.com
Chan
Selena
S
Berlin
Andrew A
AA
D000803:Angiotensin I / Q000032:analysis*; D001704:Biopolymers / Q000032:analysis* / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000032:analysis*; D008967:Molecular Biology / Q000379:methods; D015336:Molecular Probe Techniques; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods
D001704:Biopolymers; D003838:Deoxyadenine Nucleotides; C012873:2'-deoxy-5'-adenosine monophosphate; D000803:Angiotensin I