Direct characterization of the folded, unfolded and urea-denatured states of the C-terminal domain of the ribosomal protein L9.
The stability of the isolated C-terminal domain of the ribosomal protein L9 (CTL9) is strongly dependent upon pH. Below pH 4.2, the folded and unfolded states are both populated significantly. Their interconversion is slow on the NMR chemical shift time-scale and separate, well-resolved resonances from each state are observed. This allows the hydrodynamic properties of both states to be studied under identical conditions by using pulse field gradient NMR experiments. Hydrodynamic radii of the folded, unfolded and urea denatured protein molecules at pD 3.8 have been derived. The acid-denatured protein has a significantly smaller hydrodynamic radius, 28.2A, compared to that of the urea-denatured protein, which is 33.6A at pD 3.8. Far-UV CD spectra show that there is more residual secondary structure retained in the acid-denatured ensemble than in the urea-denatured one. ANS binding experiments and analysis of the CD data show that this acid-denatured species is not a molten globule state. Diffusion measurements of CTL9 were conducted over the pD range from 2.1 to 7.0. The hydrodynamic radii of both the folded and the acid-unfolded protein start to increase below pD 4, with the radius of hydration of the acid-unfolded state increasing from 25.1A at pD 4.2 to 33.5A at pD 2.1. The hydrodynamic radius of the urea-denatured protein is much less sensitive to pH. The unfolded protein at pD 2.1, no urea, has almost the same hydrodynamic radius as the urea-denatured protein at pD 3.8. The CD spectra, however, show significant differences in residual secondary structure, and the acid-denatured state contains more structure.
Li
Ying
Y
Department of Chemistry, State University of New York at Stony Brook, NY 11794-3400, USA.
Picart
Francis
F
Raleigh
Daniel P
DP
D000143:Acids / Q000494:pharmacology; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D002942:Circular Dichroism; D001411:Geobacillus stearothermophilus / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019906:Nuclear Magnetic Resonance, Biomolecular; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000494:pharmacology*
D000143:Acids; D000817:Anilino Naphthalenesulfonates; D012269:Ribosomal Proteins; C049650:ribosomal protein L9; D014508:Urea