title: Lipooligosaccharide-independent alteration of cellular homeostasis in Neisseria meningitidis-infected epithelial cells.
abstract: Neisseria meningitidis (MC) is an important cause of meningitis and septic shock. Primary loose attachment of MC to host epithelial cells is mediated by type IV pili. Lipooligosaccharide (LOS), opacity (Opa) proteins and glycolipid adhesins facilitate subsequent tight attachment. MC infection causes numerous changes in host epithelial cell homeostasis. These include cortical plaque formation, increased expression of proinflammatory cytokines and alterations in host iron homeostasis. Using both biochemical and genetic approaches, we examined the role of LOS in mediating these events. We first examined specific cellular iron homeostasis changes that occur following addition of purified MC LOS to epithelial cells. Using an MC mutant that completely lacks LOS (MC lps tbp), we examined pili-mediated attachment and cortical plaque formation in human endocervical epithelial cells (A431). We also tested whether the lack of LOS alters cellular homeostasis, including changes in the levels of host stress response factors and proinflammatory cytokines. MC lps tbp elicited the formation of cortical plaques in A431 cells. However, the plaques were less pronounced than those formed by the MC parent. Surprisingly, the proinflammatory cytokine TNF(alpha) was upregulated during infection in MC lps tbp-infected cells. Furthermore, alterations in iron homeostasis, including lower transferrin receptor 1 (TfR-1) levels, altered TfR-1 trafficking, an 'iron-starvation' gene expression profile and low iron regulatory protein (IRP) binding activity are independent of LOS. Our results demonstrate that LOS is partially involved in both the attachment to host cells and formation of cortical plaques. However, TNFalpha induction and changes in iron homeostasis observed in MC-infected epithelial cells are independent of LOS.
authors.affiliation: Vaccine and Gene Therapy Institute, Portland, OR, USA. bonnahr@ohsu.edu
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonnah
authors.forename: Jenny
authors.initials: J
authors.lastname: Hoelter
authors.forename: Liana
authors.initials: L
authors.lastname: Steeghs
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Enns
authors.forename: Magdalene
authors.initials: M
authors.lastname: So
authors.forename: Martina U
authors.initials: MU
authors.lastname: Muckenthaler
mesh_terms: D001422:Bacterial Adhesion; D004705:Endocytosis; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035925:Iron-Regulatory Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D009154:Mutation; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014168:Transferrin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D035925:Iron-Regulatory Proteins; D008070:Lipopolysaccharides; D014168:Transferrin; D014409:Tumor Necrosis Factor-alpha