Durancin L28-1A, a new bacteriocin from Enterococcus durans L28-1, isolated from soil.
OBJECTIVE
To isolate, characterize and identify bacteriocins from lactic acid bacteria in soil.


RESULTS
Thirty-four acid-producing bacteria were isolated from 87 soil samples. Antibacterial activities were detected, and one strain, L28-1 produced a bacteriocin that was active against some Gram-positive bacteria. L28-1 was identified as Enterococcus durans by 16S rDNA sequence analysis and API50CHL. This bacteriocin did not lose its activity after autoclaving (121 degrees C for 15 min), but was inactivated by protease K. The bacteriocin was purified by hydrophobic column chromatography, and Sep-Pak C(18). Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the partially purified bacteriocin contained numerous protein bands. Two bands that displayed antibacterial activities were c. 3.4 and 2.5 kDa in size. In this work, the 3.4-kDa bacteriocin was analysed with N-terminal amino acid and DNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. The results indicated that the 3.4-kDa bacteriocin of Ent. durans L28-1 is a new natural enterocin variant.


CONCLUSIONS
Enterococcus durans L28-1 produced a new bacteriocin.


CONCLUSIONS
This study reports a novel bacteriocin that is produced by Ent. durans that has potential for use as a food preservative.
Yanagida
F
F
The Institute of Enology and Viticulture, University of Yamanashi, Kitashin, Kofu, Yamanashi, Japan. yanagida@yamanashi.ac.jp
Chen
Y
Y
Onda
T
T
Shinohara
T
T
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D016983:Enterococcus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007564:Japan; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D019286:Endopeptidase K