title: XAGE-1 mRNA expression in prostate cancer and antibody response in patients.
abstract: To evaluate the feasibility of cancer vaccine targeting XAGE-1, we investigated the expression of 4 XAGE-1 transcript variants and the humoral immune response to XAGE-1 in prostate cancer patients. XAGE-1a, b, c, d mRNA expression was analyzed in 54 prostate cancer specimens and 8 specimens of benign prostate hypertrophy (BPH) by reverse transcription-polymerase chain reaction (RT-PCR). The humoral response to XAGE-1 was investigated in sera obtained from 278 patients with prostate cancer and 40 healthy volunteers by enzyme-linked immunosorbent assay (ELISA) using recombinant protein. XAGE-1b mRNA expression was observed in 14 of 54 (26%) prostate cancer specimens, while XAGE-1a, c, and d mRNA expressions were observed in 1, 1, and 3, respectively. None of the 4 XAGE-1 transcript variants was observed in the 8 BPH specimens. Antibody against XAGE-1 was detected in sera from 2 of 129 stage D2 patients, whereas none of sera from 149 patients with localized prostate cancer or lymph node metastasis had detectable XAGE-1 antibody. No reactivity to XAGE-1 was found in sera from the 40 healthy individuals.
authors.affiliation: Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Japan.
authors.forename: Fumihito
authors.initials: F
authors.lastname: Koizumi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Saika
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Sato
authors.forename: Ali Mohamed Ali
authors.initials: AM
authors.lastname: Eldib
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Nasu
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kumon
authors.forename: Eiichi
authors.initials: E
authors.lastname: Nakayama
mesh_terms: D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C413656:XAGE1A protein, human