Extent of iron pick-up in deforoxamine-coupled polyurethane materials for therapy of chronic wounds.
Polyurethane net substrates (PNS) coupled with deferoxamine (DFO) have been studied to determine the extent of Fe2+ pick-up for use in chronic wound therapy. A m solution of ferrous sulphate (FeSO4) was used to generate ferrous ions similar to those found in chronic wounds. The concentration of Fe as a function of position through the dressings was evaluated using a variety of techniques. Atomic force microscopy (AFM) and energy-filtered transmission electron microscopy (EFTEM) revealed a rough precipitated layer at the surface of activated PNS exposed to FeSO4 solution. Optical microscopy (OM) and backscattered environmental scanning electron microscopy (ESEM) showed a clear layer of Fe(3+)-enriched material in the surface regions exposed to DFO. The penetration depth of DFO into activated dressings was found to be 20-30 microm. Energy-dispersive X-ray (EDX) analysis was used to approximate the distribution of bound- and unbound-Fe as a function of position within BPNS and DFO-activated dressings after immersing them in a FeSO4 solution for various times. These studies have shown the activity of iron with respect to ionic state in DFO-activated PNS for potential using as dressing for chronic wounds.
Taylor
Jennifer E
JE
Department of Materials Science, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK. m.take@kuhp.kyoto-u.ac.jp
Laity
Peter R
PR
Hicks
John
J
Wong
Steven S
SS
Norris
Keith
K
Khunkamchoo
Peck
P
Johnson
Anthony F
AF
Cameron
Ruth E
RE
D000269:Adhesives; D001458:Bandages; D003676:Deferoxamine / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D005296:Ferrous Compounds / Q000737:chemistry; D006397:Hematinics / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D007477:Ions; D007501:Iron / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D046032:Microscopy, Energy-Filtering Transmission Electron; D008956:Models, Chemical; D018384:Oxidative Stress; D011140:Polyurethanes / Q000737:chemistry; D012542:Scattering, Radiation; D013455:Sulfur / Q000737:chemistry; D014945:Wound Healing; D014965:X-Rays
D000269:Adhesives; D005296:Ferrous Compounds; D006397:Hematinics; D007093:Imidazoles; D007477:Ions; D011140:Polyurethanes; C020748:ferrous sulfate; D013455:Sulfur; C029899:imidazole; D007501:Iron; D003676:Deferoxamine