title: Production of glucosinolate hydrolysis products in Farsetia aegyptia suspension cultures following elicitation.
abstract: Levels of glucosinolates in Farsetia aegyptia var. ovalis suspension cultures were monitored after treatment with yeast extract, chitosan, methyl jasmonate, ampicillin, and Phytophthora infestans autoclaved mycelia as elicitors. Glucosinolates were identified, and an estimation of their levels obtained from their hydrolysis products. Yeast extract improved glucotropaeolin (benzyl-glucosinolate) and glucocheirolin [3-(methylsulfonyl)propyl-glucosinolate] accumulation, and production of sec-butyl, isobutylglucosinolate and gluconasturtiin (2-phenylethyl-glucosinolate) was only detected in yeast elicited cultures. Increases were shown in glucotropaeolin levels in cultures elicited using methyljasmonate, and chitosan and methyljasmonate in combination.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Al-Gendy
authors.forename: G B
authors.initials: GB
authors.lastname: Lockwood
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000667:Ampicillin / Q000494:pharmacology; D019607:Brassicaceae / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005961:Glucosinolates / Q000378:metabolism*; D006868:Hydrolysis; D054883:Oxylipins; D010838:Phytophthora; D012441:Saccharomyces cerevisiae; D013997:Time Factors
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; D000667:Ampicillin; C072239:methyl jasmonate; D048271:Chitosan