Blood flow: a key regulatory component of corpus luteum function in the cow.
Prostaglandin F2alpha (PGF2alpha) is the primary luteolysin in the cow. During the early luteal phase, the corpus luteum (CL) is resistant to the luteolytic effect of PGF2alpha. Once mature, the CL becomes responsive to PGF2alpha and undergoes luteal regression. These actions of PGF2alpha coincide with changes in luteal blood flow (BF): PGF2alpha has no effect on BF in the early CL, but acutely increases BF in the peripheral vasculature of the mature CL within 30 min of PGF2alpha injection. During spontaneous luteolysis, luteal BF increases on Days 17-18 of the estrous cycle, prior to any decrease in plasma progesterone (P). The increase in luteal BF is synchronous with an increase in plasma PGFM levels, suggesting that pulsatile release of PGF2alpha from uterus stimulates the increase in luteal BF. Serial biopsies of these CL showed that mRNA expression for endothelial nitric oxide synthase (eNOS) together with endothelin-1 (ET-1) and angiotensin converting enzyme (ACE) increases on Days 17-18 when the luteal BF is elevated. On Day 19 when plasma P level firstly decreases, eNOS mRNA returns to the basal level whereas ET-1 and ACE mRNA remains elevated. Cyclooxygenase-2 (COX-2) mRNA expression increases on Day 19. In support of these data, an in vivo microdialysis study revealed that luteal ET-1 and angiotensin II (Ang II) secretion increases and precedes PGF2alpha secretion during spontaneous luteolysis. In conclusion, we show for the first time that an acute increase of BF occurs in the peripheral vasculature of the mature CL together with increases in eNOS expression and ET-1 and Ang II secretion in the CL during the early stages of luteolysis in the cow. We propose that the increase in luteal BF may be induced by NO from large arterioles surrounding the CL, and simultaneously uterine or exogenous PGF2alpha directly increases ET-1 and Ang II secretion from endothelial cells of microcapillary vessels within the CL, thereby suppressing P secretion by luteal cells. Taken together, our results indicate that an acute increase in luteal BF occurs as a first step of luteolysis in response to PGF2alpha. Therefore, local BF plays a key role to initiate luteal regression in the cow.
Miyamoto
A
A
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080-8555 Obihiro, Japan. akiomiya@obihiro.ac.jp
Shirasuna
K
K
Wijayagunawardane
M P B
MP
Watanabe
S
S
Hayashi
M
M
Yamamoto
D
D
Matsui
M
M
Acosta
T J
TJ
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001783:Blood Flow Velocity; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000098:blood supply* / Q000502:physiology*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood / Q000502:physiology; D019332:Endothelin-1 / Q000235:genetics; D005260:Female; D015870:Gene Expression; D003341:Luteolysis / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000235:genetics; D052250:Nitric Oxide Synthase Type III; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D019332:Endothelin-1; D012333:RNA, Messenger; D000804:Angiotensin II; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D015237:Dinoprost; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D007703:Peptidyl-Dipeptidase A