Recombinant non-structural 1 (NS1) protein of dengue-2 virus interacts with human STAT3beta protein.
A combination of yeast two-hybrid library screening, co-immunoprecipitation and immunofluorescence microscopy demonstrated that dengue-2 virus non-structural 1 (NS1) protein can interact with an N-terminally truncated form of human STAT3beta (DeltaN40-STAT3beta) protein. The NS1 protein interacted with the activated STAT3beta protein in vesicle-like structures in the cell cytoplasm. In addition, transfection of dendritic cells with plasmid expressing NS1 protein also resulted in significant induction of tumor necrosis factor-alpha (TNFalpha) and interleukin-6 (IL-6). Since the STAT3beta protein is an acute-phase response factor, its interaction with NS1 protein may influence the pathological changes observed in dengue fever, dengue hemorrhagic fever and dengue shock syndrome.
Chua
John Jia-En
JJ
Programme in Infectious Diseases and Flavivirology Laboratory, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Kent Ridge, Singapore 117597, Singapore.
Bhuvanakantham
Raghavan
R
Chow
Vincent Tak-Kwong
VT
Ng
Mah-Lee
ML
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003713:Dendritic Cells; D003716:Dengue Virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; C079340:NS1 protein, Dengue virus type 2; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C494087:Stat3 protein, mouse; D015534:Trans-Activators; D017361:Viral Nonstructural Proteins