Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer.
Gold nanoparticles with unique optical properties may be useful as biosensors in living whole cells. Using a simple and inexpensive technique, we recorded surface plasmon resonance (SPR) scattering images and SPR absorption spectra from both colloidal gold nanoparticles and from gold nanoparticles conjugated to monoclonal anti-epidermal growth factor receptor (anti-EGFR) antibodies after incubation in cell cultures with a nonmalignant epithelial cell line (HaCaT) and two malignant oral epithelial cell lines (HOC 313 clone 8 and HSC 3). Colloidal gold nanoparticles are found in dispersed and aggregated forms within the cell cytoplasm and provide anatomic labeling information, but their uptake is nonspecific for malignant cells. The anti-EGFR antibody conjugated nanoparticles specifically and homogeneously bind to the surface of the cancer type cells with 600% greater affinity than to the noncancerous cells. This specific and homogeneous binding is found to give a relatively sharper SPR absorption band with a red shifted maximum compared to that observed when added to the noncancerous cells. These results suggest that SPR scattering imaging or SPR absorption spectroscopy generated from antibody conjugated gold nanoparticles can be useful in molecular biosensor techniques for the diagnosis and investigation of oral epithelial living cancer cells in vivo and in vitro.
El-Sayed
Ivan H
IH
Department of Otolaryngology-Head and Neck Surgery, Comprehensive Cancer Center, University of California at San Francisco, San Francisco, California 94143, USA. ielsayed@ohns.ucsf.edu
Huang
Xiaohua
X
El-Sayed
Mostafa A
MA
D000936:Antigen-Antibody Complex / Q000032:analysis / Q000648:ultrastructure; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D015336:Molecular Probe Techniques; D009062:Mouth Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D043942:Nanotubes / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*
D000936:Antigen-Antibody Complex; D014408:Biomarkers, Tumor; D004815:Epidermal Growth Factor