title: Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines.
abstract: Mast cells (MCs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. Whereas the biology of rodent and human MCs has been extensively studied using in vitro derived populations, the role of MCs in pigs has not yet been evaluated, given the very low availability of pure porcine MCs populations. In the present report, we describe an original method to obtain continuous factor-dependent normal pig MCs (PMC) lines from fetal hematopoietic progenitors. These Stem Cell Factor (SCF) and Interleukin-3- (IL-3)-dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal, although immature, MCs, including metachromatic granules containing sulfated polysaccharides, the expression of c-kit and high-affinity IgE receptors (FcepsilonRI), and the ability to store histamine that is released upon cross-linking of FcepsilonRI. In vitro derived PMC lines might thus be valuable tools to further investigate the reactivity of these elements towards several parasites frequently encountered in pig, such as, but not limited to, Ascaris suum, Trichinella spiralis or Trichuris suis, or towards antigens derived from these pathogens.
authors.affiliation: UMR-BIPAR, INRA-ENVA-AFSSA-Paris XII, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons Alfort cedex, France. ffemenia@vet-alfort.fr
authors.forename: Françoise
authors.initials: F
authors.lastname: Femenia
authors.forename: Michel
authors.initials: M
authors.lastname: Arock
authors.forename: Laurence
authors.initials: L
authors.lastname: Leriche
authors.forename: Claude
authors.initials: C
authors.lastname: Delouis
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Millet
authors.forename: Nadine
authors.initials: N
authors.lastname: Ben Hamouda
authors.forename: Martine
authors.initials: M
authors.lastname: Cote
authors.forename: Annie
authors.initials: A
authors.lastname: Alliot
authors.forename: Thomas
authors.initials: T
authors.lastname: Lilin
authors.forename: Alain
authors.initials: A
authors.lastname: Pinton
authors.forename: Nathalie
authors.initials: N
authors.lastname: Iannucceli
authors.forename: André Laurent
authors.initials: AL
authors.lastname: Parodi
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D019070:Cell Lineage / Q000502:physiology*; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006632:Histamine / Q000378:metabolism; D007621:Karyotyping; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure*; D018895:Microsatellite Repeats / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D017455:Receptors, IgE; D006632:Histamine; D019009:Proto-Oncogene Proteins c-kit