A pre-S/S CHO-derived hepatitis B virus vaccine protects woodchucks from WHV productive infection.
We evaluated whether a non-adjuvanted vaccine derived from Chinese hamster ovary cells was capable of providing protection against woodchuck hepatitis virus (WHV). Three woodchucks were vaccinated with four 50-microg doses and challenged with a previously characterized virus isolate (WHV197). In all three animals, titre levels of antibodies against hepatitis B surface antigens (anti-HBs) exceeded 10 mIU/ml, peaking at 150 mIU/ml. Challenge resulted in productive acute infection in the two non-vaccinated woodchucks yet in none of the vaccinated woodchucks. In the vaccinated animals, there was evidence of abortive infection. The results demonstrate that a human vaccine is able to protect woodchucks from WHV infection.
Argentini
Claudio
C
Viral Hepatitis Unit, Department of Infectious, Parasitic, and Immune-Mediated Disease, Istituto Superiore di Sanità, Viale Regina Elena, 00161 Rome, Italy.
Giuseppetti
Roberto
R
D'Ugo
Emilio
E
La Sorsa
Valentina
V
Tritarelli
Elena
E
Orobello
Sara
S
Canitano
Andrea
A
Glück
Reinhard
R
Rapicetta
Maria
M
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000821:virology; D008392:Marmota; D016133:Polymerase Chain Reaction; D014766:Viremia
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines