title: Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry.
abstract: Peptides were phosphonylated at their N-termini by reacting with ethoxyphenylphosphinate in the presence of triethylamine and tetrachloromethane under mild conditions. The phosphonylated peptides were analyzed by tandem electrospray ionization mass spectrometry. N-Terminal phosphonylation selectively increased the intensities of b(n)-type ions relative to other ion types. The resulting simplified mass spectra clearly show the sequential loss of amino acid residues from the C-termini of peptides, providing a convenient and rapid method for peptide sequencing.
authors.affiliation: Key Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
authors.forename: Jiangyin
authors.initials: J
authors.lastname: Bao
authors.forename: Huiwang
authors.initials: H
authors.lastname: Ai
authors.forename: Hua
authors.initials: H
authors.lastname: Fu
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
mesh_terms: D063065:Organophosphonates / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D063065:Organophosphonates; D010455:Peptides