title: High-pressure effects on the proteolytic enzymes of sea bass (Dicentrarchus labrax L.) fillets.
abstract: High-pressure processing is a nonthermal technique ensuring food product safety and enabling a longer shelf life. The purpose of this experiment was to evaluate the effect of high pressure on the main proteolytic enzymes involved in fish muscle degradation during storage. Enzymes were extracted with sarcoplasmic proteins from Dicentrarchus labrax sea bass white muscle. Activity of cathepsins B, D, H, and L was quantified in protein extract, whereas calpain activity was evaluated after isolation from its endogenous inhibitor. High-pressure processing up to 500 MPa enhanced the activity of cathepsin B, H, and L, whereas the activity of cathepsin D increased up to 300 MPa and decreased above 300 MPa. With regard to calpain activity, high-pressure processing led to a decrease of activity, which was zero above 400 MPa. We suggest a leading explanation based on simultaneous deactivation of enzymes and an increase of liberation from lysosomes for cathepsins and on dissociation of subunits for calpains.
authors.affiliation: UMR CNRS 6144, Département Génie des Procédés Alimentaires, Ecole Nationale d'Ingénieurs des Techniques des Industries Agricoles et Alimentaires, BP 82225, 44322, Nantes Cedex 3, France.
authors.forename: Romuald
authors.initials: R
authors.lastname: Chéret
authors.forename: Christine
authors.initials: C
authors.lastname: Delbarre-Ladrat
authors.forename: Marie
authors.initials: M
authors.lastname: de Lamballerie-Anton
authors.forename: Véronique
authors.initials: V
authors.lastname: Verrez-Bagnis
mesh_terms: D000818:Animals; D001492:Bass; D002154:Calpain / Q000378:metabolism; D002403:Cathepsins / Q000032:analysis / Q000378:metabolism; D005511:Food Handling / Q000379:methods*; D008247:Lysosomes / Q000201:enzymology; D008460:Meat; D009132:Muscles / Q000201:enzymology* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011312:Pressure
chemical_list: D002403:Cathepsins; D010447:Peptide Hydrolases; D002154:Calpain