Evaluation of a modified reverse line blot assay for detection and typing of human papillomavirus.
Detection and typing of human papillomaviruses (HPVs) are requested by clinicians with growing frequency as part of the overall management of patients. A new method using consensus L1 amplification and a reverse line blot hybridization detection method has been described for broad spectrum HPV genotype identification. This method involves hybridization at 53 degrees C in a shaking water bath, which many laboratories might find cumbersome. We describe a modified hybridization step, using a dry-air incubator, that simplifies the detection protocol. Overall, comparable results were obtained by the modified, more easily performed protocol, indicating that laboratories might validate and use this modified method.
Tabrizi
Sepehr N
SN
Department of Molecular Microbiology, Microbiology and Infectious Diseases, the Royal Women's Hospital, Victoria, Australia.
Stevens
Matthew
M
Chen
Shujun
S
Rudland
Elice
E
Kornegay
Janet R
JR
Garland
Suzanne M
SM
D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology