title: Micellar electrokinetic chromatography method for the determination of sulfamethoxazole, trimethoprim and their main metabolites in human serum.
abstract: A complete analytical procedure, including sample clean-up and a micellar electrokinetic chromatographic method, is presented for the determination of sulfamethoxazole, trimethoprim, and their main metabolites by using 20 mmol L(-1) borate buffer (pH 9.3), 25 mmol L(-1) sodium dodecylsulfate, and 5% v/v acetonitrile as electrolyte. The separation was carried out at 30 kV and 20 degrees C in a fused silica capillary (60.2 cm x 75 microm inner diameter) fitted with a window in the capillary cartridge of 100 x 800 microm. The detector response was linear from the limit of quantification to 3 mg L(-1) for the individual components. The limits of quantification ranged from 0.13 up to 0.24 mg L(-1). The method was applied to human serum, previously spiked at different concentrations of all the analytes, and recoveries between 95% and 108% were obtained.
authors.affiliation: Regional Institute for Applied Scientific Research, University of Castilla-La Mancha, Avenida de Camilo José Cela, 10, 13071 Ciudad Real, Spain. juanjose.berzas@uclm.es
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Berzas Nevado
authors.forename: Gregorio
authors.initials: G
authors.lastname: Castañeda Peñalvo
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Guzmán Bernardo
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013420:Sulfamethoxazole / Q000097:blood* / Q000378:metabolism; D014295:Trimethoprim / Q000097:blood* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D014295:Trimethoprim; D013420:Sulfamethoxazole