title: Two-week oral toxicity study of 1,4-Dichloro-2-nitrobenzene in rats and mice.
abstract: Subacute toxicity of 1,4-dichloro-2-nitrobenzene (DCNB) was examined by feeding F344 rats and BDF1 mice of both sexes a diet containing DCNB at 625, 1250, 2500, 5000 or 10,000 ppm (w/w) for 2 weeks. All DCNB-fed rats survived to the end of the 2-week administration period, but 2 male and 6 female mice fed 10,000 ppm died during this period. The subacute toxicity was characterized by lesions affecting the liver, kidney, testis and hematopoietic system. The liver was the most responsive to DCNB, as evidenced by a dose-related increase in relative liver weight in rats and mice and centrilobular hypertrophy of hepatocytes in mice. An alteration in liver-associated lipid metabolism was suggested from the concomitant increases in serum concentrations of total cholesterol and phospholipid. A lower confidence limit of the benchmark dose yielding the response with 10% extra risk (BMDL10) for the relative liver weight indicated that rats were more responsive to DCNB than mice. The kidney lesion was characterized by alpha2upsilon-globulin-accumulated hyaline droplets in the renal tubular epithelial cells only in male rats, as indicated by positive anti-alpha2upsilon-globulin immunohistochemical staining. Testicular and hematopoietic lesions appeared at higher dose levels than did the liver and kidney lesions.
authors.affiliation: Japan Bioassay Research Center, Japan Industrial Safety and Health Association, 2445 Hirasawa, Hadano, Kanagawa 257-0015, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Aiso
authors.forename: Michiharu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Heihachiro
authors.initials: H
authors.lastname: Arito
authors.forename: Kasuke
authors.initials: K
authors.lastname: Nagano
authors.forename: Seigo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Taijiro
authors.initials: T
authors.lastname: Matsushima
mesh_terms: D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D004396:Coloring Agents; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D005260:Female; D006410:Hematopoiesis; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016273:Occupational Exposure / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D013737:Testis / Q000473:pathology
chemical_list: D004396:Coloring Agents; D004137:Dinitrochlorobenzene