Identification of a novel HLA-A*0278 allele in a Chinese family.
A novel human leukocyte antigen-A (HLA-A) allele, A*0278, has been identified in a Chinese family using DNA-based typing and molecular cloning methods. The alleles A*0278 differs from its closest matching HLA sequence of A*0256 by a silent substitution at 102 A > C and by two replacement substitutions, 98T > A and 292 C > G in exon 2, resulting in a change of codon 33 from Phe (TTC) to Tyr (TAC) and codon 98 from His (CAC) to Asp (GAC). Serology study revealed that A*0278 is associated with HLA-A2 broad specificity. A polymerase chain reaction-sequence-specific primers-based assay was developed to identify A*0278. Family study indicated that the propositus inhered his father's HLA haplotype A*0278, B*35, DRB1*15. No further individuals of A*0278 were found in 5000 Chinese bone marrow donor volunteers.
Ji
Y
Y
Shanghai Blood Center, Shanghai, China.
Sun
J-L
JL
Du
K-M
KM
Xie
J-H
JH
Ji
Y-H
YH
Yang
J-H
JH
Fu
M
M
Sun
Y
Y
Jin
Y
Y
Liu
D-Z
DZ
Zhao
T-M
TM
D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D002681:China; D003001:Cloning, Molecular; D003062:Codon; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D005192:Family Health; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D014019:Tissue Donors
D003062:Codon; D017931:DNA Primers; D015234:HLA-A Antigens