title: Longevity-associated NADH dehydrogenase subunit-2 polymorphism and serum electrolyte levels in middle-aged obese Japanese men.
abstract: Mitochondrial DNA 5178 cytosine/adenine polymorphism, which is also called NADH dehydrogenase subunit-2 237 leucine/methionine (ND2-237 Leu/Met) polymorphism is associated with Japanese longevity. This polymorphism is widely associated with blood pressure, serum lipid levels, hematological parameters, intraocular pressure, and serum protein fraction levels. However, there have been no reports on the association between ND2-237 Leu/Met polymorphism and serum electrolyte levels. To investigate this relationship, we performed an association study in 321 healthy middle-aged Japanese men. Crude data showed that serum sodium levels and serum chloride levels were significantly lower in men with ND2-237 Met than in those with ND2-237 Leu (P = 0.021 and 0.003, respectively). Cigarette consumption and body mass index were significantly and positively associated with serum chloride levels (P = 0.002 and 0.008, respectively) and hemoglobin levels were significantly and negatively associated with them (P = 0.007) in ND2-237 Leu genotypic men. In men with ND2-237 Met, only hemoglobin levels were significantly and negatively associated with serum chloride levels (P = 0.025). After adjusting for covariates, only in male obese (body mass index> or =25) subjects, serum sodium and chloride levels remained significantly lower, and serum calcium levels appeared to be significantly higher in ND2-237 Met than in ND2-237 Leu (P = 0.013, <0.001, and 0.046, respectively). Longevity-associated NADH dehydrogenase subunit-2 polymorphism may influence serum electrolyte levels in middle-aged obese Japanese men.
authors.affiliation: Department of Public Health, Kyorin University School of Medicine, Mitaka-shi, Tokyo, Japan. dawn@kyorin-u.ac.jp
authors.forename: Akatsuki
authors.initials: A
authors.lastname: Kokaze
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Naomi
authors.initials: N
authors.lastname: Matsunaga
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshida
authors.forename: Ryuji
authors.initials: R
authors.lastname: Makita
authors.forename: Masao
authors.initials: M
authors.lastname: Satoh
authors.forename: Koji
authors.initials: K
authors.lastname: Teruya
authors.forename: Kanako
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Masuda
authors.forename: Matsuko
authors.initials: M
authors.lastname: Harada
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Uchida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D044466:Asian People; D020134:Catalytic Domain / Q000235:genetics; D002712:Chlorides / Q000097:blood*; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D006801:Humans; D007564:Japan; D008136:Longevity / Q000235:genetics* / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009245:NADH Dehydrogenase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D002712:Chlorides; D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase