title: [Detecting haplotypes of four Y-STR by multiplex amplification].
abstract: OBJECTIVE
  To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.
  
  
  METHODS
  DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.
  
  
  RESULTS
  When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.
  
  
  CONCLUSIONS
  The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.
authors.affiliation: Faulty of Forensic Medicine, Zhong shan Medical College, Zhong shan University, Guangzhou 510089, China.
authors.forename: Qiu-ling
authors.initials: QL
authors.lastname: Liu
authors.forename: De-jian
authors.initials: DJ
authors.lastname: Lu
authors.forename: Ni-Mei
authors.initials: NM
authors.lastname: Xu
mesh_terms: D000483:Alleles; D002681:China; D041322:Chromosomes, Human, Y; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016622:Silver Staining; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D004247:DNA