Tubulin polyglutamylase enzymes are members of the TTL domain protein family.
Polyglutamylation of tubulin has been implicated in several functions of microtubules, but the identification of the responsible enzyme(s) has been challenging. We found that the neuronal tubulin polyglutamylase is a protein complex containing a tubulin tyrosine ligase-like (TTLL) protein, TTLL1. TTLL1 is a member of a large family of proteins with a TTL homology domain, whose members could catalyze ligations of diverse amino acids to tubulins or other substrates. In the model protist Tetrahymena thermophila, two conserved types of polyglutamylases were characterized that differ in substrate preference and subcellular localization.
Janke
Carsten
C
Centre de Recherches de Biochimie Macromoléculaire, CNRS, 34293 Montpellier, France.
Rogowski
Krzysztof
K
Wloga
Dorota
D
Regnard
Catherine
C
Kajava
Andrey V
AV
Strub
Jean-Marc
JM
Temurak
Nevzat
N
van Dijk
Juliette
J
Boucher
Dominique
D
van Dorsselaer
Alain
A
Suryavanshi
Swati
S
Gaertig
Jacek
J
Eddé
Bernard
B
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D020134:Catalytic Domain; D002923:Cilia / Q000502:physiology; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009068:Movement; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013379:Substrate Specificity; D016808:Tetrahymena thermophila / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D011099:Polyglutamic Acid; D010453:Peptide Synthases; C112698:tubulin polyglutamylase