title: Effects of cold preservation on the lung mechanical properties in rats.
abstract: In lung transplantation, cold preservation is an important process. However, the mechanical changes in the airway and tissue during cold preservation, especially before reperfusion, are unknown. To test the hypothesis that the mechanical changes in the airway and lung parenchyma start during cold preservation, we investigated the mechanical properties of the rat lung as a whole organ and in excised lung strips. In the 0 h group, the lungs were not preserved. In the 9 and 24 h group, the lungs were preserved for 9 and 24 h at 4 degrees C. After preservation, we evaluated the static compliance (Csta) of the whole lung as obtained from the pressure volume curves (n=5 in each group). Also, we measured the input impedance taken by a computer-controlled small-animal ventilator (n=9 in each group). All data were analyzed using a homogeneous linear model, which includes airway resistance (Raw), tissue elastance (H), and tissue resistance (G). Hysteresivity (eta) was calculated as G/H. Moreover, the tissue elasticity (Eqs) obtained from the quasi-static stress-strain curves was compared. There was no significant difference in Csta among the three groups. Raw was significantly lower in the 24 h group than in the 0 h group (p<0.01). Eqs was significantly higher in the preserved groups than in the 0 h group (p<0.01). These results demonstrated that the changes in the three mechanical properties of the airway and the tissue started within 9 h of preservation.
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ikeyama
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Omasa
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Hamakawa
authors.forename: T
authors.initials: T
authors.lastname: Fujinaga
authors.forename: T
authors.initials: T
authors.lastname: Fukuse
authors.forename: H
authors.initials: H
authors.lastname: Wada
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation; D005109:Extracellular Matrix / Q000502:physiology; D066298:In Vitro Techniques; D008168:Lung / Q000502:physiology*; D008170:Lung Compliance / Q000502:physiology*; D016040:Lung Transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012121:Respiration, Artificial; D013314:Stress, Mechanical