title: Growth profiles of recent canine distemper isolates on Vero cells expressing canine signalling lymphocyte activation molecule (SLAM).
abstract: Fresh samples of lymph node, lung and cerebrum taken post mortem from dogs no. 1, 2 and 3 yielded canine distemper virus (CDV) strains 007 Lm, 009 L and 011 C, respectively. These were titrated on Vero cells stably expressing canine signalling lymphocyte activation molecule (SLAM; Vero-DST cells). Growth curves of the three strains were produced by titration of the released virus and cell-associated virus at various timepoints. All three isolates, especially 007 Lm, grew well on Vero-DST cells. The titres of cell-associated virus of two strains (009 L and 011 C) were clearly lower than those of virus released into the culture supernate. The results indicate that Vero-DST cells are not only useful for primary isolation but also efficient for titrating virus from fresh tissues and for the study of growth profiles of recent CDV isolates.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: N T
authors.initials: NT
authors.lastname: Lan
authors.forename: R
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Sugano
authors.forename: S
authors.initials: S
authors.lastname: Tateyama
mesh_terms: D000818:Animals; D015703:Antigens, CD; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D004217:Distemper Virus, Canine / Q000502:physiology*; D004283:Dog Diseases / Q000473:pathology / Q000821:virology*; D004285:Dogs; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014709:Vero Cells / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary
chemical_list: D015703:Antigens, CD; D006023:Glycoproteins; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1