Iron release and oxidative DNA damage in splenic toxicity of aniline.
Mechanisms by which aniline produces selective toxicity to the spleen are not well understood. Previously, studies showed that aniline exposure induces lipid peroxidation and protein oxidation in the spleen. The present study was aimed to determine the release of free iron and oxidative DNA damage in the spleen following aniline exposure. To achieve this, male SD rats were orally administered 1 mmol/kg/d aniline for 7 d, while controls received the vehicle only. Total splenic iron content showed a significant increase of 200% in the aniline-treated rats, whereas free iron (low-molecular-weight chelatable iron) showed a marked increase of 375% in comparison to controls. Oxidative DNA damage, measured in terms of 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, showed a remarkable increase of 83% in the aniline-treated rats. These results suggest an association between release of free iron and oxidative DNA damage, which could lead to mutagenic and/or carcinogenic responses in the spleen.
Wu
Xiaohong
X
Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
Kannan
Subburaj
S
Ramanujam
V M Sadagopa
VM
Khan
M Firoze
MF
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000814:Aniline Compounds / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism
D000814:Aniline Compounds; D002273:Carcinogens; D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D003849:Deoxyguanosine; C023650:aniline