[Sensitivity of methods of titration of the vaccine strain of porcine fever virus].
Methods of titration of the CS vaccine strain of classical swine fever virus were compared in vitro and vivo. The titration in the TL and PK-15 cell culture without cytopathic effect is based on the detection of virus antigen by labeled antibodies. The infection intensity in the cell culture virtually correlated with the antigenic and immunogenic activity of dry vaccine used for swine.
Koritskaia
M A
MA
Demkina
M M
MM
Vlasova
A N
AN
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000517:prevention & control; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D007273:Injections, Intramuscular; D009195:Peroxidase; D012756:Sheep; D013552:Swine; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000592:standards*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014765:Viral Vaccines; D009195:Peroxidase