title: Effect of (Ala8,13,18)-magainin II amide on human trophoblast cells in vitro.
abstract: Magainins are cationic peptides with anti-bacterial and anti-tumor properties. The anti-nidatory function of a synthetic analogue of magainin, (Ala8,13,18)-magainin II amide, has earlier been reported, and it has been indicated that placental trophoblast cells could be a target of magainin resulting in its contragestational action. The aim of the present study was to examine the effect of (Ala8,13,18)-magainin II amide (100 ng/ml and 1000 ng/ml) on attachment efficiency, viability, differentiation in terms of hCG secretion and invasive function of isolated first trimester, human placental trophoblast cells grown on rat-tail collagen type-I matrix in primary cell culture. In the present experimental model, magainin was not found to affect human trophoblast cell functions in vitro.
authors.affiliation: Department of Physiology, All India Institute of Medical Sciences, New Delhi - 110 029.
authors.forename: P
authors.initials: P
authors.lastname: Lamba
authors.forename: Manisha
authors.initials: M
authors.lastname: Kar
authors.forename: Jayasree
authors.initials: J
authors.lastname: Sengupta
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
mesh_terms: D000577:Amides / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D010455:Peptides / Q000494:pharmacology*; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000577:Amides; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C415246:alanine(8,13,18)-magainin 2 amide