A role for brain-derived neurotrophic factor in B cell development.
In the present study, we demonstrated a significant reduction of B lymphocytes in the blood, spleen and bone marrow of BDNF deficient mice. The observed developmental block in bone marrow B cell development was linked specifically to the Pre-BII stage. B lymphocytes express the BDNF receptors p75NTR and TrkB(gp95), while no BDNF expression was found. However, a strong BDNF expression was demonstrated in bone marrow stromal cells. An increase of intracellular free calcium [Ca2+]i in B lymphocytes after BDNF application confirms a direct responsiveness of B lymphocytes to BDNF. In conclusion, these results suggest a role of BDNF for normal B lymphocyte development through paracrine effects in the bone marrow.
Schuhmann
Berit
B
Department of Clinical Chemistry and Molecular Diagnostics, University Hospital, Philipps-Universität, Baldingerstrasse, 35033 Marburg, Germany.
Dietrich
Alexander
A
Sel
Serdar
S
Hahn
Christian
C
Klingenspor
Martin
M
Lommatzsch
Marek
M
Gudermann
Thomas
T
Braun
Armin
A
Renz
Harald
H
Nockher
Wolfgang Andreas
WA
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology / Q000473:pathology; D038041:Lymphopoiesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology / Q000473:pathology
D019208:Brain-Derived Neurotrophic Factor