title: Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy.
abstract: To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation. In comparison with sham-operated rats, the left ventricular (LV) weight of the aortic-banded rats increased progressively. At 28 days after surgery, the expression of DGKepsilon mRNA but not DGKzeta or PAP2b mRNA in the LV myocardium significantly decreased in the aortic-banded rats compared with the sham-operated rats. DGKzeta protein in the LV myocardium translocated from the particulate to the cytosolic compartment in the aortic-banded rats. Furthermore, the myocardial content of 1,2-diacylglycerol and PKCdelta protein expression in the particulate fraction of the LV myocardium significantly increased in aortic-banded rats compared with sham-operated rats. These results suggest that DGKepsilon and DGKzeta play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.
authors.affiliation: Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yahagi
authors.forename: Morihiko
authors.initials: M
authors.lastname: Takeda
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Asaumi
authors.forename: Kenji
authors.initials: K
authors.lastname: Okumura
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Takahashi
authors.forename: Jun
authors.initials: J
authors.lastname: Takahashi
authors.forename: Jun
authors.initials: J
authors.lastname: Ohta
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Minatoya
authors.forename: Masahito
authors.initials: M
authors.lastname: Sakuma
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
authors.forename: Kunio
authors.initials: K
authors.lastname: Shirato
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kagaya
mesh_terms: D000818:Animals; D001794:Blood Pressure; D006332:Cardiomegaly / Q000150:complications / Q000201:enzymology*; D019852:Diacylglycerol Kinase / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018487:Ventricular Dysfunction, Left / Q000201:enzymology* / Q000209:etiology
chemical_list: D007527:Isoenzymes; D019852:Diacylglycerol Kinase; D010711:Phosphatidate Phosphatase