title: Collagen gel droplet culture method to examine in vitro chemosensitivity.
abstract: For effective cancer chemotherapy, chemosensitivity testing of anticancer drugs should be performed using fresh surgical specimens obtained from the cancer. We have developed a new in vitro chemosensitivity test named the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The CD-DST method consists of a collagen gel droplet embedded culture step, exposure and washout of anticancer drug, a serum-free culture step, and evaluation of anticancer effect by image analysis. This method has many advantages including a high success rate for primary culture, the need for only a small number of cells for the test, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system, a good correlation between in vitro and in vivo results, and simplicity and speed. The CD-DST method can be performed in the laboratory using a system kit Primaster.
authors.affiliation: Biochemical Laboratory, Nitta Gelatin Inc., Futamata, Yao-City, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003094:Collagen; D046508:Culture Techniques; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005782:Gels; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D005782:Gels; D003094:Collagen