title: A comparative study of endothelial cell markers expressed in chronically inflamed human tissues: MECA-79, Duffy antigen receptor for chemokines, von Willebrand factor, CD31, CD34, CD105 and CD146.
abstract: Endothelial cells play a central role in chronic inflammation: for example, they express adhesion molecules and present chemokines leading to enhanced leukocyte recruitment into tissues. Numerous markers of endothelial cells have been reported but there has been a lack of comparative data on their specificity. The present study compared the specificity of seven endothelial cell markers in the rheumatoid synovium and the colon of patients with Crohn's disease. These markers were: the sulphated epitope MECA-79, the Duffy antigen receptor for chemokines (DARC), von Willebrand factor, CD31 (PECAM-1), CD34, CD105 (endoglin) and CD146. MECA-79, DARC and von Willebrand factor showed a specific endothelial cell distribution. MECA-79, which recognizes sulphated ligands for leukocyte adhesion receptor L-selectin (CD62L), was selective for a subset of venules in highly inflamed tissue and was present in rheumatoid but not control osteoarthritic synovia. DARC was also specific for venules but had a more widespread distribution than MECA-79, and was present in rheumatoid and control synovia. The other markers all labelled endothelial cells in venules, arterioles and capillaries. However, they also localized to other cell types. For example, CD34 stained fibroblasts, CD146 was expressed by the pericytes and smooth muscle cells of vessel walls and CD31 and CD105 labelled a broad range of cell types.
authors.affiliation: Endocube S.A.S., Prologue Biotech-BP-700, Rue Pierre et Marie Curie, 31319 Labege Cedex, France. jim.middleton@rjah.nhs.uk
authors.forename: Jim
authors.initials: J
authors.lastname: Middleton
authors.forename: Laure
authors.initials: L
authors.lastname: Americh
authors.forename: Regis
authors.initials: R
authors.lastname: Gayon
authors.forename: Denis
authors.initials: D
authors.lastname: Julien
authors.forename: Michel
authors.initials: M
authors.lastname: Mansat
authors.forename: Pierre
authors.initials: P
authors.lastname: Mansat
authors.forename: Philippe
authors.initials: P
authors.lastname: Anract
authors.forename: Alain
authors.initials: A
authors.lastname: Cantagrel
authors.forename: Pierre
authors.initials: P
authors.lastname: Cattan
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Reimund
authors.forename: Luc
authors.initials: L
authors.lastname: Aguilar
authors.forename: Francois
authors.initials: F
authors.lastname: Amalric
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Girard
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34 / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001168:Arthritis / Q000473:pathology*; D001172:Arthritis, Rheumatoid / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D051929:CD146 Antigen; D003106:Colon / Q000737:chemistry / Q000473:pathology; D003424:Crohn Disease / Q000473:pathology*; D004375:Duffy Blood-Group System / Q000032:analysis*; D000071063:Endoglin; D042783:Endothelial Cells / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins; D019006:Neural Cell Adhesion Molecules / Q000032:analysis; D020370:Osteoarthritis, Knee / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis*; D013583:Synovial Membrane / Q000737:chemistry / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis*
chemical_list: C473745:ACKR1 protein, human; D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; D015415:Biomarkers; D051929:CD146 Antigen; D004375:Duffy Blood-Group System; C064206:ENG protein, human; D000071063:Endoglin; C069886:L-selectin counter-receptors; C497902:MCAM protein, human; D008565:Membrane Proteins; D019006:Neural Cell Adhesion Molecules; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D014841:von Willebrand Factor