title: Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide.
abstract: This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of casein hydrolysate was used to increase the concentration of peptide-bound amino acid in the circulation. Samples of mammary gland tissue from each goat were taken by biopsy at the end of each treatment period to measure gene and protein expression of aminopeptidase N in the tissue. There were no measurable effects on feed intake and milk production for any of the treatments. Western blot analysis showed that aminopeptidase N is located on the basolateral side of parenchymal cells and not on the apical membranes. Abomasal infusion of casein hydrolysate caused a marked change in the profile of arterial blood free amino acids and peptide-bound amino acids smaller than 1500 Da. Abundance of aminopeptidase N mRNA and protein increased by 51 and 58%, respectively, in casein hydrolysate-infused goats compared with the control treatment. It was concluded that aminopeptidase N is one candidate actively involved in the mammary gland to support protein synthesis and milk production. In accordance with the nutritional conditions in the current experiment, it is suggested that aminopeptidase N expression is partly controlled by the metabolic requirements of the gland and postabsorptive forms of amino acids in the circulation.
authors.affiliation: Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot, Israel. Mabjeesh@agri.huji.ac.il
authors.forename: S J
authors.initials: SJ
authors.lastname: Mabjeesh
authors.forename: O
authors.initials: O
authors.lastname: Gal-Garber
authors.forename: J
authors.initials: J
authors.lastname: Milgram
authors.forename: Y
authors.initials: Y
authors.lastname: Feuermann
authors.forename: M
authors.initials: M
authors.lastname: Cohen-Zinder
authors.forename: A
authors.initials: A
authors.lastname: Shamay
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D015153:Blotting, Western; D018826:CD13 Antigens / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D002364:Caseins / Q000008:administration & dosage; D004032:Diet; D004435:Eating; D005260:Female; D015870:Gene Expression; D006041:Goats / Q000097:blood / Q000378:metabolism*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology*; D010455:Peptides / Q000097:blood*; D014176:Protein Biosynthesis
chemical_list: D000596:Amino Acids; D002364:Caseins; D010455:Peptides; C475729:casein hydrolysate; D018826:CD13 Antigens