An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens.
A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.
Thomson
Lynn M
LM
Department of Medical Microbiology, University of Aberdeen, U.K.
Traore
Hamidou
H
Yesilkaya
Hasan
H
Doig
Christine
C
Steingrimsdottir
Herdis
H
Garcia
Lucia
L
Forbes
Ken J
KJ
D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003902:Detergents; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
D004269:DNA, Bacterial; D003902:Detergents; D011933:Reagent Kits, Diagnostic