Surface modification of biodegradable polyesters with fatty acid conjugates for improved drug targeting.
We describe a general method for incorporating target ligands into the surface of biocompatible polyester poly(lactic-co-glycolic acid) (PLGA) 50/50 materials using fatty acids. Avidin-fatty acid conjugates were prepared and efficiently incorporated into PLGA. Avidin was chosen as an adaptor protein to facilitate the attachment of a variety of biotinylated ligands. We show that fatty acid preferentially associates with the hydrophobic PLGA matrix, rather than the external aqueous environment, facilitating a prolonged presentation of avidin over several weeks. We successfully applied this approach in both microspheres encapsulating a model protein, bovine serum albumin, and PLGA scaffolds fabricated by a salt-leaching method. Because of its ease, generality and flexibility, this strategy promises widespread utility in modifying the surface of PLGA-based materials for applications in drug delivery and tissue engineering.
Fahmy
Tarek M
TM
Department of Biomedical Engineering, Yale University, PO Box 208284, New Haven, CT 06520, USA. Tarek.fahmy@yale.edu
Samstein
Robert M
RM
Harness
Casey C
CC
Mark Saltzman
W
W
D020341:Absorbable Implants; D001360:Avidin / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods*; D005227:Fatty Acids / Q000737:chemistry*; D019344:Lactic Acid / Q000032:analysis / Q000737:chemistry*; D008422:Materials Testing; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000032:analysis / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000032:analysis / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005227:Fatty Acids; D011091:Polyesters; D011108:Polymers; D001360:Avidin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid