title: In situ hybridization of dihydroxyacetone phosphate acyltransferase, the regulating enzyme involved in plasmalogen biosynthesis.
abstract: In situ hybridization can be carried out using different methods. The experimenter has to choose various parameters: the type of tissue fixation, the time of incubation, and the duration of the exposure time. All these parameters are determinant for the sensitivity and the resolution of this technique. This publication of technical aspects described different experiments performed for in situ hybridization on liver tissue. We may conclude on the parameters to optimize each step of the hybridization procedure. Moreover, this technique could be transposed to the brain and applied to little structures with a light expression of DHAP-AT.
authors.affiliation: INRA, Unité de Nutrition Lipidique, 17 rue Sully, BP 86510, 21065 Dijon Cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: André
authors.forename: Christian
authors.initials: C
authors.lastname: Tessier
authors.forename: Lionel
authors.initials: L
authors.lastname: Brétillon
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Sébédio
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Chardigny
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods
chemical_list: D012333:RNA, Messenger; D000217:Acyltransferases; C020051:glycerone-phosphate O-acyltransferase