title: Protein sequence tags: a novel solution for comparative proteomics.
abstract: Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmaceutically relevant membrane and nuclear proteins. This procedure addresses a similar number of proteins, compared to the multidimensional protein identification technology approach, but offers additionally a quantitative analysis with its recently developed quantitative PST version.
authors.affiliation: Proteome Sciences R&D, Frankfurt am Main, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Kuhn
authors.forename: Thorsten
authors.initials: T
authors.lastname: Prinz
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schäfer
authors.forename: Christian
authors.initials: C
authors.lastname: Baumann
authors.forename: Marco
authors.initials: M
authors.lastname: Schärfke
authors.forename: Stefan
authors.initials: S
authors.lastname: Kienle
authors.forename: Josef
authors.initials: J
authors.lastname: Schwarz
authors.forename: Sandra
authors.initials: S
authors.lastname: Steiner
authors.forename: Christian
authors.initials: C
authors.lastname: Hamon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes; D005656:Fungal Proteins / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D002247:Carbon Isotopes; D005656:Fungal Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010455:Peptides; D020543:Proteome