Generation of serum-stabilized retroviruses: reduction of alpha1,3gal-epitope synthesis in a murine NIH3T3-derived packaging cell line by expression of chimeric glycosyltransferases.
Retroviral vectors released from mouse-derived packaging cell lines are inactivated in human sera by naturally occurring antibodies due to the recognition of Galalpha1,3Galbeta1,4GlcNAc (alphagal-epitope) decorated surface proteins. In this study, an extensive analysis of the glycosylation potential of NIH3T3-derived PA317 packaging cells using combined MALDI/TOF-MS and HPAE-PAD reveals that 34% of the N-glycan moiety represents alphagal-epitope containing structures. Stable expression of glycosyltransferases and transport signal chimeras has been demonstrated to represent an efficient tool to alter cell- and species-specific glycosylation (Grabenhorst and Conradt, 1999. J. Biol. Chem. 274, 36107-36116). In order to reduce alphagal-epitope synthesis selected chimeric glycosyltransferases were constructed by fusing Golgi-signal sequences for compartment-specific localization with the catalytic domain of alpha2,3-sialyltransferase (ST3). Stable expression of these constructs in these cells resulted in a significant reduced alphagal-epitope synthesis, and moreover, a release of retroviral vectors showing an up to 3.5-fold increase in serum stability. Thus, our results suggest that the stably transfected cells stably transfected with chimeric glycosyltransferases compete efficiently with endogenous alpha1,3-galactosyltransferase. This approach allows favored glycodesign and we anticipate the applicability of such improved retroviral vectors produced by glycosylation engineered host cells for in vivo gene therapy and, furthermore, suggest the therapeutic benefit of this technology for xenotransplantation.
Hansen
Wiebke
W
Department of Regulation and Differentiation, Molecular Biotechnology, German Research Centre of Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Grabenhorst
Eckart
E
Nimtz
Manfred
M
Müller
Katja
K
Conradt
Harald S
HS
Wirth
Manfred
M
D000818:Animals; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D012190:Retroviridae / Q000254:growth & development*; D044967:Serum / Q000821:virology*; D019065:Virus Assembly / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology; D000519:alpha-Galactosidase / Q000235:genetics / Q000378:metabolism*
D016695:Glycosyltransferases; D000519:alpha-Galactosidase