title: Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
abstract: Mice on the CBA inbred strain background expressing the well characterized mutation designated xid in the cytoplasmic signalling enzyme Bruton's protein kinase have been previously noted to illustrate shifts in T helper type 1 (Th1)/Th2 immunity which is underlined by an apparent failure to produce the regulatory cytokine interleukin-10. In the current study we examined if this extended to infection with Mycobacterium tuberculosis, which also depends on Th1 immunity. Contrary to expectations, xid mice showed evidence of a transient early susceptibility to pulmonary infection, changes in macrophage morphology, and decreased activation of lung natural killer cells, while showing evidence of substantial IL-10 production and accumulation in lung lesions macrophages, but paradoxically this did not influence the course of the chronic disease. In addition, macrophages from the lungs of xid mice also expressed high levels of CD14. These observations suggest that the xid mutation in cellular signalling has much wider effects on the immune system than previously thought.
authors.affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins 80523, USA.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Junqueira-Kipnis
authors.forename: Andre
authors.initials: A
authors.lastname: Kipnis
authors.forename: Marcela
authors.initials: M
authors.lastname: Henao Tamayo
authors.forename: Marisa
authors.initials: M
authors.lastname: Harton
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gonzalez Juarrero
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Basaraba
authors.forename: Ian M
authors.initials: IM
authors.lastname: Orme
mesh_terms: D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D005260:Female; D020022:Genetic Predisposition to Disease; D016753:Interleukin-10 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008168:Lung / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains; D009154:Mutation; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000473:pathology
chemical_list: D018950:Lipopolysaccharide Receptors; D016753:Interleukin-10; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625951:Btk protein, mouse