title: Conformational behavior of chemically reactive alanine-rich repetitive protein polymers.
abstract: The synthesis of protein-based polymers with controlled conformational properties and functional group placement offers many opportunities for the design of advanced materials. In this work, protein engineering methods have been used to produce repetitive alanine-rich protein polymers with the sequence [(AAAQ)(5)(AAAE)(AAAQ)(5)](x) (x = 2 and 6); these macromolecules may mimic architectural features of certain alanine-rich helical sequences found in natural proteins. Various proteins from this family can be readily expressed and purified from Escherichia coli. Circular dichroic spectroscopy (CD) characterization demonstrates that the purified proteins are highly helical under a variety of conditions. Thermal analysis of [(AAAQ)(5)(AAAE)(AAAQ)(5)](2) via differential scanning calorimetry (DSC) and CD indicates that the protein undergoes a reversible helix-coil transition at approximately 45 degrees C and that the protein conformation can be manipulated at elevated temperatures depending on solution conditions. The demonstrated conformational properties of these artificial proteins suggest that they may be excellent candidates for elucidating structure-function relationships in biopolymers for nanotechnology and biological applications.
authors.affiliation: Department of Materials Science and Engineeringstitute, University of Delaware, 201 Dupont Hall, Newark, Delaware 19716, USA.
authors.forename: Robin S
authors.initials: RS
authors.lastname: Farmer
authors.forename: Kristi L
authors.initials: KL
authors.lastname: Kiick
mesh_terms: D000409:Alanine / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D008969:Molecular Sequence Data; D011108:Polymers / Q000032:analysis / Q000737:chemistry*; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics
chemical_list: D011108:Polymers; D011506:Proteins; D000409:Alanine