Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines.
Mast cells (MCs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. Whereas the biology of rodent and human MCs has been extensively studied using in vitro derived populations, the role of MCs in pigs has not yet been evaluated, given the very low availability of pure porcine MCs populations. In the present report, we describe an original method to obtain continuous factor-dependent normal pig MCs (PMC) lines from fetal hematopoietic progenitors. These Stem Cell Factor (SCF) and Interleukin-3- (IL-3)-dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal, although immature, MCs, including metachromatic granules containing sulfated polysaccharides, the expression of c-kit and high-affinity IgE receptors (FcepsilonRI), and the ability to store histamine that is released upon cross-linking of FcepsilonRI. In vitro derived PMC lines might thus be valuable tools to further investigate the reactivity of these elements towards several parasites frequently encountered in pig, such as, but not limited to, Ascaris suum, Trichinella spiralis or Trichuris suis, or towards antigens derived from these pathogens.
Femenia
Françoise
F
UMR-BIPAR, INRA-ENVA-AFSSA-Paris XII, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons Alfort cedex, France. ffemenia@vet-alfort.fr
Arock
Michel
M
Leriche
Laurence
L
Delouis
Claude
C
Millet
Gaëlle
G
Ben Hamouda
Nadine
N
Cote
Martine
M
Alliot
Annie
A
Lilin
Thomas
T
Pinton
Alain
A
Iannucceli
Nathalie
N
Parodi
André Laurent
AL
Boireau
Pascal
P
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D019070:Cell Lineage / Q000502:physiology*; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006632:Histamine / Q000378:metabolism; D007621:Karyotyping; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure*; D018895:Microsatellite Repeats / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D034421:Sus scrofa
D017455:Receptors, IgE; D006632:Histamine; D019009:Proto-Oncogene Proteins c-kit