title: Huperzine A--human serum albumin association: chromatographic and thermodynamic approach.
abstract: The synthesis of six new huperzine analogues was reported. Each product presents an amidification of the free amine on huperzine A. The synthesis strategy of these new huperzine A derivatives is based on a condensation with an acyl anhydride. The binding on HSA of two molecule series (huperzine and benzodiazepine, respectively) was investigated with high performance liquid affinity chromatography (HPLAC) using an HSA column. A thermodynamic approach showed that binding huperzine A on HSA involved hydrophobic and Van der Waals interactions. A comparative thermodynamic study with benzodiazepine molecules was carried out to determine the potential binding site of huperzine derivatives on HSA.
authors.affiliation: Equipe des Sciences Séparatives et Biopharmaceutiques (2SB) EA-482, Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besançon Cedex, France.
authors.forename: François
authors.initials: F
authors.lastname: Darrouzain
authors.forename: Claire
authors.initials: C
authors.lastname: André
authors.forename: Lhassane
authors.initials: L
authors.lastname: Ismaili
authors.forename: Myriam
authors.initials: M
authors.lastname: Matoga
authors.forename: Yves Claude
authors.initials: YC
authors.lastname: Guillaume
mesh_terms: D000470:Alkaloids; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012709:Serum Albumin / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000470:Alkaloids; D012709:Serum Albumin; D012717:Sesquiterpenes; C050426:huperzine A