title: tRNA actively shuttles between the nucleus and cytosol in yeast.
abstract: Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.
authors.affiliation: Department of Chemistry, Graduate School of Science, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Takano
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshihisa
mesh_terms: D021581:Active Transport, Cell Nucleus; D000255:Adenosine Triphosphate / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D007438:Introns; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012355:RNA, Transfer, Ile / Q000378:metabolism; D012361:RNA, Transfer, Pro / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D012322:RNA Precursors; D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012346:RNA, Transfer, Amino Acyl; D012355:RNA, Transfer, Ile; D012361:RNA, Transfer, Pro; D012365:RNA, Transfer, Tyr; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D020931:ran GTP-Binding Protein