title: Enantioseparation of 2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one and its 7-chloro-derivative by capillary zone electrophoresis using native and substituted beta-cyclodextrins as chiral additives.
abstract: Efficient, rapid and inexpensive methods were established for the chiral separation of two glucopyranosyl compounds from plant extracts, by capillary zone electrophoresis (CZE). Baseline separation was achieved for both compounds. Several native cyclodextrins and their derivatives were tried as chiral selectors. CM-beta-CD and HP-beta-CD (with addition of acetonitrile in the buffer) gave rise to optimal chiral separation for the two compounds, respectively, each within a few minutes. The effects of several parameters on the chiral separation were studied.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100083, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Shi
authors.forename: Changhong
authors.initials: C
authors.lastname: Huo
authors.forename: Hongjun
authors.initials: H
authors.lastname: Yao
authors.forename: Rongfu
authors.initials: R
authors.lastname: Gao
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bingjiu
authors.initials: B
authors.lastname: Xu
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins