[DNA samples preparation from single cell and its application in sensitivity test].
OBJECTIVE
To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes.


METHODS
The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus Kit-ABI PRISM 310 system.


RESULTS
We succeed in establishing a micromanipulation method to prepare groups of DNA samples, which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus Kit-ABI310 system.


CONCLUSIONS
The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.
Deng
Jian-qiang
JQ
Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China,
Shi
Mei-sen
MS
Ying
Bing-wu
BW
D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D008853:Microscopy; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D020080:Tandem Repeat Sequences
D004247:DNA