title: Polymorphic karyotypes and sex chromosomes in the tufted deer (Elaphodus cephalophus): cytogenetic studies and analyses of sex chromosome-linked genes.
abstract: Different diploid chromosome numbers have been reported for the tufted deer Elaphodus cephalophus (female, 2n = 46/47; male, 2n = 47/48) in earlier reports. In the present study, chromosomal analysis of seven tufted deer (5 male symbol, 2 female symbol) revealed that the karyotype of these animals contains 48 chromosomes, including a pair of large heteromorphic chromosomes in the male. C-banding revealed these chromosomes to be very rich in constitutive heterochromatin. Chromosome banding and PCR of sex chromosome-linked genes (SRY, ZFX, ZFY) performed on DOP-PCR products of single microdissected X and Y chromosomes confirmed that the large telocentric chromosome without secondary constriction is the X chromosome whereas the subtelocentric chromosome is the Y. The increased size of both, the X and Y chromosome, appears to be at least partially attributable to the presence of substantial amounts of heterochromatin.
authors.affiliation: Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, P.R. of China. caoxiangrong@njnu.edu.cn
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: X
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D004268:DNA-Binding Proteins / Q000235:genetics; D003670:Deer / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D051741:Kruppel-Like Transcription Factors; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D012730:Sex Chromosomes / Q000235:genetics*; D012867:Skin / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D006570:Heterochromatin; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human; C062076:zinc finger protein, X-linked