title: No regional differences of cytochrome P450 expression in the liver of Cynomolgus monkeys (Macaca fascicularis).
abstract: Non-human primates are frequently used in toxicological studies the result of which are extrapolated to humans, but background data on drug metabolism ability among monkeys derived from different countries has not been published, especially on the key enzyme, cytochrome P450 (CYP450). We assessed the amounts of hepatic CYP450 obtained from cynomolgus monkeys of different ages and from different countries in this study. There were no regional differences of total P450 content, as well as major CYP450 isozymes (CYP 1A, 2A, 2B, 2C, 2D, 2E1 and 3A4) in cynomolgus monkeys by westernblot analysis. Similarly, there were no significant differences with hybrid cynomolgus monkeys, but variations in individual values were large. As for aging, total P450 contents declined in old cynomolgus monkeys (12-32 years of age). These results indicate the usefulness of basic data of hepatic CYP450 obtained from cynomolgus monkeys of different ages and from different countries.
authors.affiliation: Department of Biomedical Science, School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Akahori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takatori
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Itagaki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008252:Macaca fascicularis
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System