Counting absolute number of lymphocytes in quail whole blood by flow cytometry.
In a previous study, we reported a new method for counting quail blood cells. After quail blood cells were stained with fluorescent lipophilic dye (DiOC6(3)), absolute counts of erythrocytes, granulocytes, and monocytes were obtained by means of flow cytometry (FC). The FC method has the potential for application to avian blood cells count; however, the method was unable to distinguish between lymphocytes and thrombocytes. In the present study, we improved the FC method to obtain separate counts of lymphocytes using DiOC5(3). After quail blood cells were stained with DiOC5(3), the cells were measured with FC. Each blood cell type was distinguished by means of their typical FL-1 (green fluorescence) and SSC (side scatter). Absolute numbers of erythrocytes, granulocytes, monocytes and lymphocytes in whole blood were obtained. The improved FC analysis worked equally well with chicken (Gallus gallus) and goose (Anser cygnoides) blood.
Uchiyama
Rie
R
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fugisawa, Kanagawa, Japan.
Moritomo
Tadaaki
T
Kai
Osamu
O
Uwatoko
Kazuhiro
K
Inoue
Yuki
Y
Nakanishi
Teruyuki
T
D000818:Animals; D002232:Carbocyanines; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D018655:Lymphocyte Count / Q000379:methods; D008214:Lymphocytes; D011784:Quail / Q000097:blood*
D002232:Carbocyanines; D005456:Fluorescent Dyes; C018528:3,3'-dipentyl-2,2'-oxacarbocyanine