Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblast-like cells.
It is known that the excessive generation of reactive oxygen species (ROS) is a significant factor in tissue injury observed in many disease states. To determine whether extreme levels of mechanical stress applied to osteoblasts enhances ROS synthesis, we loaded cyclic tensile stretch on osteoblast-like HT-3 cells. Cyclic tensile stretch loaded on these cells clearly enhanced ROS synthesis in a time- and magnitude-dependent fashion. Cyclic tensile stretch also enhanced superoxide dismutase (SOD) activity. The disruption of microfilaments with cytochalasin D abolished the stress-induced ROS synthesis. Rotenone, an inhibitor of the mitochondrial electron transport chain, enhanced stress-induced ROS synthesis. These data suggest that actin filament and mitochondria are involved in this action.
Yamamoto
N
N
Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohnohigashi 377-2, Osaka-sayama, Osaka, 589-8511, Japan.
Fukuda
K
K
Matsushita
T
T
Matsukawa
M
M
Hara
F
F
Hamanishi
C
C
D000199:Actins / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D008928:Mitochondria / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D013314:Stress, Mechanical; D013482:Superoxide Dismutase / Q000096:biosynthesis
D000199:Actins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase