Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy.
To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation. In comparison with sham-operated rats, the left ventricular (LV) weight of the aortic-banded rats increased progressively. At 28 days after surgery, the expression of DGKepsilon mRNA but not DGKzeta or PAP2b mRNA in the LV myocardium significantly decreased in the aortic-banded rats compared with the sham-operated rats. DGKzeta protein in the LV myocardium translocated from the particulate to the cytosolic compartment in the aortic-banded rats. Furthermore, the myocardial content of 1,2-diacylglycerol and PKCdelta protein expression in the particulate fraction of the LV myocardium significantly increased in aortic-banded rats compared with sham-operated rats. These results suggest that DGKepsilon and DGKzeta play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.
Yahagi
Hirokazu
H
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
Takeda
Morihiko
M
Asaumi
Yasuhide
Y
Okumura
Kenji
K
Takahashi
Ryotaro
R
Takahashi
Jun
J
Ohta
Jun
J
Tada
Hiroko
H
Minatoya
Yutaka
Y
Sakuma
Masahito
M
Watanabe
Jun
J
Goto
Kaoru
K
Shirato
Kunio
K
Kagaya
Yutaka
Y
D000818:Animals; D001794:Blood Pressure; D006332:Cardiomegaly / Q000150:complications / Q000201:enzymology*; D019852:Diacylglycerol Kinase / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018487:Ventricular Dysfunction, Left / Q000201:enzymology* / Q000209:etiology
D007527:Isoenzymes; D019852:Diacylglycerol Kinase; D010711:Phosphatidate Phosphatase