title: Allergen-specific IgE measured by a continuous random-access immunoanalyzer: interassay comparison and agreement with skin testing.
abstract: BACKGROUND
  Our aims were to evaluate the performance of a fully automated system for measuring circulating allergen-specific IgE (sIgE) against an established in vitro assay and to assess the system's diagnostic accuracy against objective clinical criteria for identifying sensitization to specific allergens.
  
  
  METHODS
  Using both the IMMULITE 2000 Allergy system (IML) and an assay based on the widely used ImmunoCAP technology (CAP), we measured sIgE in serum samples from 169 persons with suspected allergies to airborne or insect venom allergens. Skin testing outcome served as the clinical comparison method.
  
  
  RESULTS
  Interassay classification agreement between the IML and CAP, relative to the usual allergen-specific IgE cutoff of 0.35 kIU/L, ranged from 76% (yellow jacket venom) to 95% (orchard grass); agreement was 88.3% for all 9 allergens combined (766 results). The 90 discordant results, when resolved by skin testing, showed better agreement with the IML (72%) than with the CAP (28%). Compared with skin testing, for each of the 9 allergens studied, the area under the ROC curve was at least as large for the IML as for the CAP, reflecting in part the more extensive working range of the IML (0.10-100 kIU/L vs 0.35-100 kIU/L for CAP).
  
  
  CONCLUSIONS
  Laboratory testing for sIgE can be performed on a fully automated, random-access system with an extended working range and with diagnostic accuracy for representative allergens equivalent to or better than that of the semiautomated CAP technology.
authors.affiliation: Department of Dermatology and Allergy, Biederstein, Germany. ollert@lrz.tum.de
authors.forename: Markus
authors.initials: M
authors.lastname: Ollert
authors.forename: Stephanie
authors.initials: S
authors.lastname: Weissenbacher
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rakoski
authors.forename: Johannes
authors.initials: J
authors.lastname: Ring
mesh_terms: D000485:Allergens / Q000276:immunology*; D005260:Female; D006801:Humans; D007118:Immunoassay; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D012372:ROC Curve; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D012882:Skin Tests
chemical_list: D000485:Allergens; D007073:Immunoglobulin E