title: Evaluation of a modified reverse line blot assay for detection and typing of human papillomavirus.
abstract: Detection and typing of human papillomaviruses (HPVs) are requested by clinicians with growing frequency as part of the overall management of patients. A new method using consensus L1 amplification and a reverse line blot hybridization detection method has been described for broad spectrum HPV genotype identification. This method involves hybridization at 53 degrees C in a shaking water bath, which many laboratories might find cumbersome. We describe a modified hybridization step, using a dry-air incubator, that simplifies the detection protocol. Overall, comparable results were obtained by the modified, more easily performed protocol, indicating that laboratories might validate and use this modified method.
authors.affiliation: Department of Molecular Microbiology, Microbiology and Infectious Diseases, the Royal Women's Hospital, Victoria, Australia.
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
authors.forename: Matthew
authors.initials: M
authors.lastname: Stevens
authors.forename: Shujun
authors.initials: S
authors.lastname: Chen
authors.forename: Elice
authors.initials: E
authors.lastname: Rudland
authors.forename: Janet R
authors.initials: JR
authors.lastname: Kornegay
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
mesh_terms: D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology