title: Antigenicity of ovine strains of Anaplasma phagocytophilum grown in tick cells and ovine granulocytes.
abstract: Antigens prepared from ovine granulocytes and tick cells infected with ovine strains of Anaplasma phagocytophilum, the causative agent of tick-borne fever, were tested in respect of their suitability for the assay of antibodies in ovine sera by enzyme-linked immunosorbent assay (ELISA) and by indirect immunofluorescent antibody test (IFAT). Antigens prepared from tick cells were as sensitive and specific as those expressed in ovine granulocytes for the detection of specific antibodies by ELISA, but they failed to react in the IFAT with immune sera obtained from sheep previously infected with ovine strains of A. phagocytophilum.
authors.affiliation: Department of Veterinary Pathology, University of Liverpool, Liverpool L69 3BX, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Woldehiwet
authors.forename: B K
authors.initials: BK
authors.lastname: Horrocks
mesh_terms: D041081:Anaplasma phagocytophilum / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D016873:Ehrlichiosis / Q000276:immunology / Q000635:transmission / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006098:Granulocytes / Q000276:immunology / Q000382:microbiology*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology*; D017282:Tick-Borne Diseases / Q000276:immunology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D013987:Ticks / Q000276:immunology / Q000382:microbiology*
chemical_list: D000939:Epitopes