[Determination of HLA-DRB1 gene polymorphism in Luoba ethnic group of Tibet].
OBJECTIVE
To investigate the frequencies of allele and genotype of HLA-DRB1 gene in Luoba ethnic group.


METHODS
HLA-DRB1 genes from 92 individuals of Luoba ethnic group in Tibet Autonomous region were investigated, using PCR-SSO (polymerase chain reaction -sequence specific oligo-nucleotide) genotyping methods. We compared allele frequencies of Luoba population in HLA-DRB1 locus with that of various ethnic groups distributed worldwide, constructed the phylogenetic tree by UPGMA (unweighted pair group method with arithmetic mean), and counted genetic distance by Nei measure.


RESULTS
Of the 11 DRB1 alleles detected, the two most common genes were DRBI * 04( gene frequency: 27.20% ) and DRB * 12 (25.50%) , which covered 52.70% of the total alleles detected from Luoba ethnic group. The frequencies of HLA-DRB1 * 14(15.20% ), DRB1 * 15(9.80% ) and DRB1 * 08(8.20% ) were more than 5%. Luoba was unique in the distribution of HLA alleles. The genetic distance between Luoba and Tibetan was closer than that with other population. A dendrogram based on the DRB1 genes by cluster analysis suggested that Luoba ethnic group might cluster with Tibetan firstly, then cluster with other population living in China (except Uygr) , and cluster with Caucasian and Black finally.


CONCLUSIONS
The HLA-DRBI gene frequency of Luoba individuals in Tibet Linzhi had some differences compared with that of other Chinese population. The kindred relation between Luoba and Tibetan was closer than that with other population, which was coincident with the results of ethnology, history and sociology.
Kang
Long-li
LL
Key Laboratory of Environment and Genes Related to Disease, Xi'an Jiaotong University, Xi'an 710061 , China.
Gao
Fang
F
Zhang
Hong-bo
HB
Yuan
Dong-ya
DY
Zhao
Feng-cang
FC
Li
Sheng-bin
SB
D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D010802:Phylogeny; D011110:Polymorphism, Genetic; D018609:Tibet / Q000208:ethnology
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains