title: Analysis of oxidative DNA damage 8-hydroxy-2'-deoxyguanosine as a biomarker of exposures to persistent pollutants for marine mammals.
abstract: An oxidative DNA biomarker, 8-hydroxy-2'-deoxyguanosine (8-OHdG), was determined in the livers and kidneys of stranded or by-caught cetaceans along the Taiwan coast through isotope-dilution liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) to evaluate the feasibility of analyzing the DNA adduct in marine mammals and then to study the association between 8-OHdG and levels of polychlorinated biphenyls (PCBs) and dichlorodiphenyldichloroethylene (DDE) in the blubbers of the cetaceans. The mean values of the 8-OHdG from the liver and kidney samples were 19.83+/-10.00 pmol/micromol deoxyguanosine (dG) (6.90-53.53 pmol/micromol dG) and 19.16+/-7.48 pmol/micromol dG (5.36-39.36 pmol/micromol dG), respectively. In general, 8-OHdG was not related to the general health status of the by-caught and stranded animals and also was not related to species. However, the levels of 8-OHdG had a positive correlation with concentrations of PCBs, but not DDE, in female cetacean livers. In addition, when selected coplanar PCBs (dioxin-like congeners) were used to compare the 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQs) with 8-OHdG of by-caught cetaceans, a high positive correlation (r = 0.80, p < 0.01) was found in mature female animals. Thus, the detection of 8-OHdG in marine mammals with isotope-dilution LC/MS/ MS is possible, and the study of the relationship between oxidative DNA damage and environmental contaminants under natural exposure indicates that the level of 8-OHdG in female cetacean livers is associated with coplanar PCBs and the factor of sexual maturity.
authors.affiliation: Department of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chi-Shan
authors.initials: CS
authors.lastname: Li
authors.forename: Kuen-Yuh
authors.initials: KY
authors.lastname: Wu
authors.forename: Gou-Ping
authors.initials: GP
authors.lastname: Chang-Chien
authors.forename: Chin-Cheng
authors.initials: CC
authors.lastname: Chou
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002591:Cetacea / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D003633:Dichlorodiphenyl Dichloroethylene / Q000032:analysis / Q000633:toxicity; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007554:Isotopes; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity; D012741:Sexual Maturation / Q000187:drug effects; D013624:Taiwan
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D007554:Isotopes; D003633:Dichlorodiphenyl Dichloroethylene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D011078:Polychlorinated Biphenyls; D003849:Deoxyguanosine