Cytotoxicity of phenolic acid phenethyl esters on oral cancer cells.
Many phenolic acid phenethyl esters possess diverse biological effects including anti-cancer activity. A series of 14 derivatives were synthesized for the evaluation of their cytotoxic effect on oral cancer cells. These derivatives were tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric and trypan blue dye exclusion assay on the growth of oral squamous cell carcinoma (SAS), oral epidermoid carcinoma-Meng 1 (OEC-M1), and normal human oral fibroblast (NHOF) cells, respectively. Caffeic acid phenethyl esters, 3a (CAPE), and 3b, 3c, and 3d showed cytotoxic effects on the SAS and OEC-M1 cell lines, but not the NHOF cell line at a 5-100 microM dose range. Flow cytometric analysis showed that 3c caused OEC-M1 cell arrest at G2/M phase. Such differential effects on representative cancer and normal cells suggested these compounds might be useful in oral cancer chemotherapy.
Lee
Ya-Ting
YT
Department of Pharmacology, School of Medicine, National Yang-Ming University, 155, Sec. 2, Li-Nung St. Peitou, Taipei 112, Taiwan, ROC.
Don
Ming-Jaw
MJ
Hung
Pei-Shih
PS
Shen
Yuh-Chiang
YC
Lo
Yin-Shen
YS
Chang
Kuo-Wei
KW
Chen
Chieh-Fu
CF
Ho
Li-Kang
LK
D002109:Caffeic Acids / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014062:Tongue Neoplasms / Q000188:drug therapy* / Q000473:pathology
D002109:Caffeic Acids; D062385:Hydroxybenzoates; C055494:caffeic acid phenethyl ester; C017616:phenolic acid; D010626:Phenylethyl Alcohol