title: Comparison of in vitro cytotoxicity of Fusarium mycotoxins,deoxynivalenol, T-2 toxin and zearalenone on selected human epithelial cell lines.
abstract: Three human epithelial cell lines (CaCo-2, HEp-2 and HeLa) implicated as potential targets for three Fusarium toxins were tested for the extent of survival on exposure to increasing toxin concentration and incubation periods. Cytotoxicity assay using 3(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) was carried out with deoxynivalenol (DON), T-2 toxins and zearalenone (ZON) on CaCo-2, HEp-2 and HeLa cell lines. Of the three cell lines used, HeLa was the most sensitive, eliciting cell death after 2 days exposure at 100 ng ml(-1)with T-2 toxin. HeLa was the only cell line to exhibit cytotoxicity towards ZON showing cell death at 1000 ng ml(-1)after 2 days which increased to 4 days, showing substantial cell death at 200 ng ml(-1). HEp-2 was sensitive to DON showing cell death after 2 days (100 ng ml(-1)) with complete cell death occurring at 200 ng ml(-1) after 4 days of exposure. Substantial cytoxicity of T-2 towards HEp-2 occurred after 2 days at 1000 ng ml(-1) and complete cell death occurred with 100 ng ml(-1) at day 4. The CaCo-2 cell line was generally resistant to the mycotoxins tested between 100 and 1000 ng ml(-1). This study shows that cytotoxicity of Fusarium toxins to epithelium cell lines is concentration- and time- dependant and results from ZON-HeLa interaction indicate possible cell type-mycotoxin specificity.
authors.affiliation: School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, G4 0BA, UK.
authors.forename: T W
authors.initials: TW
authors.lastname: Calvert
authors.forename: K E
authors.initials: KE
authors.lastname: Aidoo
authors.forename: A G G
authors.initials: AG
authors.lastname: Candlish
authors.forename: A R Mohd
authors.initials: AR
authors.lastname: Fuat
mesh_terms: D018938:Caco-2 Cells; D016923:Cell Death; D004847:Epithelial Cells; D006367:HeLa Cells; D006801:Humans; D013605:T-2 Toxin / Q000633:toxicity; D013997:Time Factors; D014255:Trichothecenes / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D014255:Trichothecenes; D015025:Zearalenone; D013605:T-2 Toxin; C007262:deoxynivalenol