Characterization of a novel HLA-Cw*02 variant, Cw*0208, in a Caucasian individual.
We describe an additional HLA-Cw*02 variant, HLA-Cw*0208, which has been identified in a renal transplant recipient of Caucasian origin (Italy). After performing preliminary serological typing, we analyzed exons 2 and 3 of the HLA-C locus polymorphism by cloning the amplified DNA and using a sequence-based typing method. The new allele differs from Cw*020202 by one nucleotide substitution at nucleotide 61 (G-->A) of exon 2, which translates to a difference of one amino acid at residue 21 (His-->Arg) of the HLA-C heavy chain. We propose that Cw*0208 was generated by a random point mutation in codon 21 from the Cw*020202 allele, or through gene conversion of Cw*020202 with another allele, probably the Cw*1205 and Cw*1602 alleles.
Canossi
A
A
CNR Institute, Organ Transplant and Immunocitology, 67100 L'Aquila, Italy. a.canossi@itoi.cnr.it
Aureli
A
A
Piancatelli
D
D
Liberatore
G
G
Del Beato
T
T
Ozzella
G
G
Tessitore
A
A
Piazza
A
A
Papola
F
F
Casciani
C U
CU
Adorno
D
D
D000483:Alleles; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001483:Base Sequence; D005091:Exons; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D017354:Point Mutation; D011110:Polymorphism, Genetic; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D015236:HLA-C Antigens; C048767:HLA-C*02 antigen