title: [Investigation of TT virus-DNA in multitransfused children and healthy children].
abstract: TT virus (TTV) is a naked, single stranded DNA virus, which has been discovered in the serum of a patient with posttransfusion hepatitis of unknown etiology. TTV is widespread in the population, however, the mode of its transmission is unclear. This study was conducted to search for TTV-DNA positivity rates and its relationship with the clinical outcomes of recipients who underwent multiple blood or blood product transfusion, together with healthy children. TTV-DNA was investigated in 52 multitransfused pediatric patients (age range: 3 mnths - 17.5 yrs, mean age: 9.2 +/- 5.7 years) and 18 healthy children (age range: 1 mnth - 16.5 yrs, mean age: 8.1 +/- 4.9 years), by qualitative in-house semi-nested polymerase chain reaction (PCR) with the primers NG059, NG061 and NG063, generated from ORF1 region of the viral genome. TTV-DNA was found positive in 30.8% of multitransfused, and 16.7% of healthy children. The differences of TTV-DNA positivity rates between the multitransfused and control groups, and ALT values between the patients with positive and negative TTV-DNA, were statistically insignificant (p>0.05). As a result, no relationship was detected between TTV positivity and hepatitis, although there was a statistically insignificant increase of TTV-DNA positivity in multitransfused children. However, since the primers of ORF1 N22 region used in our PCR method did not have enough sensitivity for the detection of TTV-DNA, it has been concluded that more sensitive primers such as UTR primers, should be used for more reliable evaluation of the results.
authors.affiliation: Osmangazi Universitesi Tip Fakültesi, Cocuk Sagligi ve Hastaliklari Anabilim Dali, Eskişehir.
authors.forename: Coşkun
authors.initials: C
authors.lastname: Yarar
authors.forename: Ozcan
authors.initials: O
authors.lastname: Bör
authors.forename: Tercan
authors.initials: T
authors.lastname: Us
authors.forename: Yurdanur
authors.initials: Y
authors.lastname: Akgün
authors.forename: Necat A
authors.initials: NA
authors.lastname: Akgün
mesh_terms: D000293:Adolescent; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001803:Blood Transfusion / Q000706:statistics & numerical data*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004266:DNA Virus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission*; D004279:DNA, Viral / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D006801:Humans; D007223:Infant; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D065227:Transfusion Reaction; D014421:Turkey / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase