title: [Gene reversion of induced differentiation of adult human bone marrow-derived mesenchymal stem cells into neuron-like cells].
abstract: OBJECTIVE
  To observe gene reversion during differentiation of human adult bone marrow-derived mesenchymal stem cells (MSCs) into neuron-like cells induced by Shenqiye.
  
  
  METHODS
  The MSCs were separated, cultured and expanded in the culture medium and induced to differentiate into neuron-like cells with Shenqiye. The expressions of neuron-specific enolase (NSE), neurofilament (NF), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemical method, and the changes of 10 genes of the cells after differentiation were detected by reverse transcriptional PCR.
  
  
  RESULTS
  The MSCs exhibited neuronal phenotype when treated with Shenqiye and the neuron-like cells were positive for expressions of NSE and NF but not for glial astrocyte marker GFAP. After withdrawal of Shenqiye from the medium, the neuron-like cells were reversed to MSC, flat or spindal in morphology. The gene expression profiles of the redifferentiated cells were similar to those of undifferentiated MSCs.
  
  
  CONCLUSIONS
  Shenqiye can induce MSC differentiation into neuron-like cells during and after which gene reversion may occur.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Yao
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Shan-wei
authors.initials: SW
authors.lastname: Feng
authors.forename: Hui-yu
authors.initials: HY
authors.lastname: Feng
authors.forename: Zu-guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Yu-bin
authors.initials: YB
authors.lastname: Deng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016900:Neurofilament Proteins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology*; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis
chemical_list: D004365:Drugs, Chinese Herbal; D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase