tRNA actively shuttles between the nucleus and cytosol in yeast.
Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.
Takano
Akira
A
Department of Chemistry, Graduate School of Science, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Endo
Toshiya
T
Yoshihisa
Tohru
T
D021581:Active Transport, Cell Nucleus; D000255:Adenosine Triphosphate / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D007438:Introns; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012355:RNA, Transfer, Ile / Q000378:metabolism; D012361:RNA, Transfer, Pro / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic; D020931:ran GTP-Binding Protein / Q000378:metabolism
D012322:RNA Precursors; D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012346:RNA, Transfer, Amino Acyl; D012355:RNA, Transfer, Ile; D012361:RNA, Transfer, Pro; D012365:RNA, Transfer, Tyr; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D020931:ran GTP-Binding Protein