Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate.
In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in Fibrobacter succinogenes S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by (1)H-NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin-1-phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.
Nouaille
Régis
R
Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 Université Blaise Pascal - CNRS, 63177 Aubière cedex, France.
Matulova
Maria
M
Delort
Anne-Marie
AM
Forano
Evelyne
E
D002475:Cellobiose / Q000378:metabolism; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003912:Dextrins / Q000096:biosynthesis* / Q000737:chemistry; D045848:Fibrobacter / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry
D003470:Culture Media; D003912:Dextrins; D011134:Polysaccharides; D002475:Cellobiose; D008320:Maltose; C008315:maltodextrin; D002482:Cellulose; C068994:cellodextrin; D005947:Glucose