Techniques for antifungal susceptibility testing of Candida albicans biofilms.
Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces. Cells in biofilms display phenotypic properties that are radically different from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, Candida biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly reproducible microtiter plate-based assay for the antifungal susceptibility testing of C. albicans biofilms. Because of its simplicity, compatibility with widely available 96-well microplate platform, high throughput, and automation potential, this assay represents an important tool towards the standardization of in vitro antifungal susceptibility testing of fungal biofilms.
Ramage
Gordon
G
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Scotland, UK.
López-Ribot
José Luis
JL
D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D003124:Colorimetry / Q000379:methods; D025141:Drug Resistance, Fungal; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards
D000935:Antifungal Agents