[Nano flow multidimensional liquid chromatography for proteome analysis of rat liver].
A nano flow comprehensive multidimensional liquid chromatography was developed. By using this separation platform, complex samples can be injected, desalted, separated and analyzed in complete automatization. Coupling of the strong cation-exchange (SCX) with capillary reversed-phase liquid chromatography (cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. The flow rate through the nano scale analytical column during the separation process was maintained at 200 nL/min. The peak capacity for the nano-2D-LC was up to 620 under these conditions.
Wang
Yan
Y
Department of Chemistry, Fudan University, Shanghai 200032, China.
Gao
Mingxia
M
Gu
Xue
X
Zhang
Xiangmin
X
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D030562:Databases, Protein; D008099:Liver / Q000737:chemistry*; D036103:Nanotechnology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats
D020543:Proteome