Quantitating adenylate nucleotides in diverse organisms.
Quantitation of cellular adenylate levels (i.e., ATP, ADP, AMP) has widespread applications in physiological, metabolic and energetic studies. We have compared classical adenylate extraction procedures (i.e., perchloric acid, boiling) with a previously unreported proteinase K-based extraction technique. Our results suggest that all three techniques are comparable in soft animal tissue, but proteinase K-based extractions consistently generated higher adenylate yields from a broad range of organisms, particularly those containing a cell wall (e.g., alga, bacteria, fungi, plant).
Napolitano
Michael J
MJ
Biology Department, 315 Penn Street, Rutgers, The State University of New Jersey, Camden, NJ 08102, USA.
Shain
Daniel H
DH
D000227:Adenine Nucleotides / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000302:isolation & purification; D000249:Adenosine Monophosphate / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002696:Chlamydomonas / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019286:Endopeptidase K / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry; D031315:Geranium / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry; D010472:Perchlorates
D000227:Adenine Nucleotides; D010472:Perchlorates; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019286:Endopeptidase K