title: Determination of acetonitrile and cyanide in rat blood: application to an experimental study.
abstract: Methods were developed for the analysis of acetonitrile and its metabolite cyanide in the blood of rats exposed to acetonitrile. Acetonitrile was analyzed by the headspace technique coupled to gas chromatography with detection by flame ionization, and cyanide was analyzed by high-performance liquid chromatography with fluorescence detection (lambdaex = 418 nm and lambdaem = 460 nm) after derivatization of the ion with naphthalene 2,3-dicarboxyaldehyde and taurine. The quantitation limits of the methods for the analysis of acetonitrile and cyanide were 4.875 microg/mL and 0.025 microg/mL, respectively. The coefficients of variation of 10% or less obtained for intra- and interassay precision indicate the precision of these analytical methods and the systematic errors, all less than 5%, indicate that the methods are quite accurate. The methods were applied to an experimental study after the animals received acetonitrile at the doses of 2 mmol/kg or 5 mmol/kg.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, São Paulo, Brasil.
authors.forename: Fabiano Henrique
authors.initials: FH
authors.lastname: Mateus
authors.forename: José Salvador
authors.initials: JS
authors.lastname: Lepera
authors.forename: Vera Lucia
authors.initials: VL
authors.lastname: Lanchote
mesh_terms: D000097:Acetonitriles / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003486:Cyanides / Q000097:blood* / Q000378:metabolism; D005410:Flame Ionization; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
chemical_list: D000097:Acetonitriles; D003486:Cyanides; C032159:acetonitrile