title: TRAV gene usage in pig T-cell receptor alpha cDNA.
abstract: Pig (Sus scrofa) TRA clones were isolated from cDNA libraries of total RNA from two different sources, the thymus of a 1-month-old LW strain pig and the peripheral blood lymphocytes of a 5-month-old Clawn strain pig. Among 103 complete TRA cDNA clones from both sources, 33 different TRAV genes were identified. By comparing their sequence identities against one another, these pig TRAV genes were grouped into 20 subgroups, including 13 subgroups, each containing only a single member. All of these pig subgroups gave corresponding human and mouse functional counterparts, suggesting their functional commonality. An exception was the Va01 gene segment, which lacked a functional human counterpart. The present report provides groundwork for studies on pig TRA expression.
authors.affiliation: Genome Research Department, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, 305-8602, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Hirohide
authors.initials: H
authors.lastname: Uenishi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hatsuse
authors.forename: Eimei
authors.initials: E
authors.lastname: Sato
authors.forename: Takashi
authors.initials: T
authors.lastname: Awata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasue
authors.forename: Yohtaroh
authors.initials: Y
authors.lastname: Takagaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015723:Gene Library; D014644:Genetic Variation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta