title: Evaluation of liver and peripheral blood micronucleus assays with 9 chemicals using young rats. A study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Japanese Environmental Mutagen Society (JEMS)-Mammalian Mutagenicity Study Group (MMS).
abstract: We conducted simultaneous liver and peripheral blood micronucleus assays in young rats with seven rodent hepatocarcinogens-4,4'-methylenedianiline (MDA), quinoline, o-toluidine, 4-chloro-o-phenylenediamine (CPDA), dimethylnitrosamine (DMN), p-dimethylaminoazobenzene (DAB), and di(2-ethylhexyl)phthalate (DEHP)-and two mutagenic chemicals-kojic acid and methylmethanesulfonate (MMS). Quinoline, DMN, and DAB were positive in the liver assay, while o-toluidine, kojic acid, DAB, and MMS were positive in the peripheral blood assay. o-Toluidine, kojic acid, and DAB are reportedly negative in mouse bone marrow micronucleus assays, indicating a species difference. Our results revealed a correlation between micronucleus induction in hepatocytes and hepatocarcinogenicity. This technique can be useful for the detection of micronucleus-inducing chemicals that require metabolic activation, and it enables simultaneous comparison of the micronucleus-inducing potential of chemicals in the liver and peripheral blood in the same individual.
authors.affiliation: Ina Research Inc., 2148-188 Nishiminowa, Ina-shi, Nagano 399-4501, Japan. h-suzuki@ina-research.co.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Naohiro
authors.initials: N
authors.lastname: Ikeda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yukari
authors.initials: Y
authors.lastname: Terashima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hagiwara
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hatakeyama
authors.forename: Koko
authors.initials: K
authors.lastname: Nagaoka
authors.forename: Junichi
authors.initials: J
authors.lastname: Yoshida
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Saito
authors.forename: Jin
authors.initials: J
authors.lastname: Tanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects*; D008741:Methyl Methanesulfonate / Q000633:toxicity; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009602:Nitrosamines / Q000633:toxicity; D010655:Phenylenediamines / Q000633:toxicity; D011753:Pyrones / Q000633:toxicity; D011804:Quinolines / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D014052:Toluidines / Q000633:toxicity; D004124:p-Dimethylaminoazobenzene / Q000633:toxicity; D001559:Benzhydryl Compounds
chemical_list: D000814:Aniline Compounds; D002273:Carcinogens; D004051:Diethylhexyl Phthalate; D008741:Methyl Methanesulfonate; D009153:Mutagens; D009602:Nitrosamines; D010655:Phenylenediamines; D011753:Pyrones; D011804:Quinolines; D014052:Toluidines; D004124:p-Dimethylaminoazobenzene; C011890:kojic acid; C015060:4-chloro-1,2-diaminobenzene; C023622:2-toluidine; C037219:quinoline; C009505:4,4'-diaminodiphenylmethane; D001559:Benzhydryl Compounds