title: Adeno-associated virus type 2 enhances goose parvovirus replication in embryonated goose eggs.
abstract: The autonomous goose parvovirus (GPV) and the human helper-dependent adeno-associated virus type 2 (AAV2) share a high degree of homology. To determine if this evolutionary relationship has a biological impact, we studied viral replication in human 293 cells and in embryonated goose eggs coinfected with both viruses. Similar experiments were performed with the minute virus of mice (MVM), an autonomous murine parvovirus with less homology to AAV2. In human 293 cells, both GPV and MVM augmented AAV2 replication. In contrast, AAV2 markedly enhanced GPV replication in embryonated goose eggs under conditions where a similar effect was not observed with MVM. AAV2 did not replicate in embryonated goose eggs and AAV2 inactivated by UV-irradiation also enhanced GPV replication. To our knowledge, this is the first report that a human helper-dependent member of the Parvoviridae can provide helper activity for an autonomous parvovirus in a natural host.
authors.affiliation: Kimron Veterinary Institute, Beit Dagon, Israel. malkins@agri.huji.ac.il
authors.forename: Mertyn
authors.initials: M
authors.lastname: Malkinson
authors.forename: Ernest
authors.initials: E
authors.lastname: Winocour
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000821:virology; D005777:Geese; D006378:Helper Viruses / Q000502:physiology*; D006801:Humans; D017992:Parvovirus / Q000502:physiology*; D014779:Virus Replication