title: Patient-specific embryonic stem cells derived from human SCNT blastocysts.
abstract: Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated.
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. hwangws@snu.ac.kr
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Roh
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Dae Kee
authors.initials: DK
authors.lastname: Kwon
authors.forename: Sue
authors.initials: S
authors.lastname: Kim
authors.forename: Sun Jong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Kwon
authors.forename: Chang Kyu
authors.initials: CK
authors.lastname: Lee
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Jin Mee
authors.initials: JM
authors.lastname: Kim
authors.forename: Curie
authors.initials: C
authors.lastname: Ahn
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Paek
authors.forename: Sang Sik
authors.initials: SS
authors.lastname: Chang
authors.forename: Jung Jin
authors.initials: JJ
authors.lastname: Koo
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Yoon
authors.forename: Jung Hye
authors.initials: JH
authors.lastname: Hwang
authors.forename: Youn Young
authors.initials: YY
authors.lastname: Hwang
authors.forename: Ye Soo
authors.initials: YS
authors.lastname: Park
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Oh
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Jong Hyuk
authors.initials: JH
authors.lastname: Park
authors.forename: Shin Yong
authors.initials: SY
authors.lastname: Moon
authors.forename: Gerald
authors.initials: G
authors.lastname: Schatten
mesh_terms: D000328:Adult; D000361:Agammaglobulinemia; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D019976:Cloning, Organism; D016172:DNA Fingerprinting; D003922:Diabetes Mellitus, Type 1; D044127:Epigenesis, Genetic; D028744:Ethics Committees, Research; D005260:Female; D005347:Fibroblasts; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007258:Informed Consent; D007621:Karyotyping; D008297:Male; D053652:Nuclear Transfer Techniques; D018587:Oocyte Donation; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000276:immunology; D013119:Spinal Cord Injuries; D033581:Stem Cell Transplantation; D009927:Tissue and Organ Procurement
chemical_list: D006680:HLA Antigens