Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas aliena type strain KMM 3562T containing two residues of L-serine in the repeating unit.
The structure of an acidic polysaccharide from Pseudoalteromonas aliena type strain KMM 3562(T) has been elucidated. The polysaccharide was studied by component analysis, (1)H and (13)C NMR spectroscopy, including 2D NMR experiments. A (1)H, (13)C band-selective constant-time heteronuclear multiple-bond connectivity experiment was used to determine amide linkages, between serine and uronic acid (UA) residues, via (3)J(H,C) correlations between Ser-alphaH and UA-C-6. It was found that the polysaccharide consists of pentasaccharide repeating units with the following structure: [carbohydrate structure]; see text.
Nordmark
Eva-Lisa
EL
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Perepelov
Andrei V
AV
Shashkov
Alexander S
AS
Nazarenko
Evgeny L
EL
Gorshkova
Raisa P
RP
Ivanova
Elena P
EP
Widmalm
Göran
G
D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D031161:Pseudoalteromonas / Q000737:chemistry* / Q000145:classification*; D012694:Serine / Q000032:analysis*
D011134:Polysaccharides; D012694:Serine