title: Suppression of macrophage infiltration into the conjunctiva by clodronate liposomes in experimental immune-mediated blepharoconjunctivitis.
abstract: Macrophages infiltrate the conjunctiva in severe cases of allergic conjunctivitis (AC) such as atopic keratoconjunctivitis (AKC). We established experimental immune-mediated blepharoconjunctivitis (EC) in Brown Norway (BN) rats as a model for severe types of AC. We investigated whether macrophage infiltration in the conjunctiva in this EC model is inhibited by clodronate liposomes (CL2MDP-lip). The numbers of ED1-positive but not ED2-positive macrophages in the conjunctivas were increased by the induction of EC. Subconjunctival injection of CL2MDP-lip decreased the number of ED2-positive but not ED1-positive macrophages in the conjunctivas of naive rats. CL2MDP-lip did not affect macrophages in the spleen. Subconjunctival injection of CL2MDP-lip into EC-developing BN rats decreased the number of ED2-positive macrophages at all the time points. ED1-positive cell infiltration was inhibited when treatment was administered just prior to OVA challenge. Intravenous injection of CL2MDP-lip decreased the number of ED2-positive cells in the conjunctiva. Thus, we conclude that CL2MDP-lip inhibits infiltration of macrophages into the conjunctiva within 24 h of antigen challenge.
authors.affiliation: Laboratory of Immunology, Department of Ophthalmology, Kochi Medical School, Nankoku-city, 783-8505 Japan. fukusima@med.kochi-u.ac.jp
authors.forename: Atsuki
authors.initials: A
authors.lastname: Fukushima
authors.forename: Akemi
authors.initials: A
authors.lastname: Ozaki
authors.forename: Waka
authors.initials: W
authors.lastname: Ishida
authors.forename: Nico
authors.initials: N
authors.lastname: van Rooijen
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Fukata
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000818:Animals; D004002:Clodronic Acid / Q000008:administration & dosage*; D003233:Conjunctivitis, Allergic / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051381:Rats
chemical_list: D008081:Liposomes; D004002:Clodronic Acid