title: [Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs].
abstract: OBJECTIVE
  To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis.
  
  
  METHODS
  Sixty female guinea pigs were randomized into 3 groups: group I (fed with normal diet), group II (fed with low-protein diet) and group III (fed with low-protein diet and Shengqing Capsules). After six-week feeding, the gallstone formation and the expressions of bilirubin UDP-glucuronosyltransferase (B-UGT) mRNA and cholesterol 7alpha-hydroxylase (CYP7A1) mRNA were observed.
  
  
  RESULTS
  The proportions of stone-formed in groups I, II and III were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of B-UGT and CYP7A1 mRNAs were higher in both group I and group III as compared with those in the group II (P<0.05).
  
  
  CONCLUSIONS
  Shengqing Capsules can reduce the rate of stone-formation, which may be due to its interference of metabolism of bilirubin and cholesterol and up-regulation of the expressions of B-UGT and CYP7A1 mRNAs.
authors.affiliation: Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Pei-Ting
authors.initials: PT
authors.lastname: Zhu
authors.forename: Jing-Zhe
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Zuo-Biao
authors.initials: ZB
authors.lastname: Yuan
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Ju
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D002214:Capsules; D002769:Cholelithiasis / Q000235:genetics / Q000517:prevention & control*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000096:biosynthesis* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D014453:Glucuronosyltransferase / Q000096:biosynthesis* / Q000235:genetics; D006168:Guinea Pigs; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D002790:Cholesterol 7-alpha-Hydroxylase; D014453:Glucuronosyltransferase; D001663:Bilirubin