title: Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex.
abstract: Compromising the activity of the spindle checkpoint permits mitotic exit in the presence of unattached kinetochores and, consequently, greatly increases the rate of aneuploidy in the daughter cells. The metazoan checkpoint mechanism is more complex than in yeast in that it requires additional proteins and activities besides the classical Mads and Bubs. Among these are Rod, Zw10, and Zwilch, components of a 700 Kdal complex (Rod/Zw10) that is required for recruitment of dynein/dynactin to kinetochores but whose role in the checkpoint is poorly understood. The dynamics of Rod and Mad2, examined in different organisms, show intriguing similarities as well as apparent differences. Here we simultaneously follow GFP-Mad2 and RFP-Rod and find they are in fact closely associated throughout early mitosis. They accumulate simultaneously on kinetochores and are shed together along microtubule fibers after attachment. Their behavior and position within attached kinetochores is distinct from that of BubR1; Mad2 and Rod colocalize to the outermost kinetochore region (the corona), whereas BubR1 is slightly more interior. Moreover, Mad2, but not BubR1, Bub1, Bub3, or Mps1, requires Rod/Zw10 for its accumulation on unattached kinetochores. Rod/Zw10 thus contributes to checkpoint activation by promoting Mad2 recruitment and to checkpoint inactivation by recruiting dynein/dynactin that subsequently removes Mad2 from attached kinetochores.
authors.affiliation: Centre National de la Recherche Scientifique, Centre de Génétique Moléculaire, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Eulalie
authors.initials: E
authors.lastname: Buffin
authors.forename: Christophe
authors.initials: C
authors.lastname: Lefebvre
authors.forename: Junyong
authors.initials: J
authors.lastname: Huang
authors.forename: Mary Elisabeth
authors.initials: ME
authors.lastname: Gagou
authors.forename: Roger E
authors.initials: RE
authors.lastname: Karess
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000378:metabolism; D049452:Green Fluorescent Proteins; D018386:Kinetochores / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D000097573:Red Fluorescent Protein
chemical_list: D018797:Cell Cycle Proteins; D017931:DNA Primers; D029721:Drosophila Proteins; D004398:Dyneins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D000097573:Red Fluorescent Protein; C064993:ROD protein, Drosophila; C076055:Zw10 protein, Drosophila; C090464:Bub1 spindle checkpoint protein