Modulation of the cytokine network in human adult astrocytes by human herpesvirus-6A.
Human herpesvirus-6A (HHV-6A) is a common pathogen whose role in CNS disorders including multiple sclerosis remains controversial. To understand how HHV-6A could influence inflammatory pathways in the CNS, we infected cultured human adult astrocytes and examined the expression of 268 cytokines, chemokines, growth factors and their receptors by gene profiling. HHV-6 infection alone had little effect on the astrocyte gene profile but strongly altered the astrocyte response to proinflammatory cytokines. Under those conditions astrocytes express higher levels of anti-inflammatory mediators including IL-10 and IL-11, chemotactic factors, growth factors and factors controlling type I interferon production. Our data suggest that HHV-6 itself does not evoke a pro-inflammatory response in astrocytes but rather triggers immune modulatory factors in the face of inflammation.
Meeuwsen
Sonja
S
Division Biomedical Research, TNO Prevention and Health, P.O. Box 2215, 2301 CE Leiden, The Netherlands.
Persoon-Deen
Carla
C
Bsibsi
Malika
M
Bajramovic
Jeffrey J
JJ
Ravid
Rivka
R
De Bolle
Leen
L
van Noort
Johannes M
JM
D001253:Astrocytes / Q000378:metabolism / Q000821:virology*; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D007211:Indoles; D008954:Models, Biological; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000235:genetics / Q000378:metabolism; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology
D016207:Cytokines; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D007211:Indoles; D018121:Receptors, Cytokine; D017978:Receptors, Growth Factor; C007293:DAPI