title: A microleakage study of single-bottle adhesives applied to enamel and cementum and aged by both occlusal loading and thermocycling.
abstract: OBJECTIVE
  To compare enamel and dentin microleakage of three single-bottle dentin-bonding agents (DBA) (One-Step, Single Bond, and Dentamed P&B) on teeth that were then subjected to thermocycling and occlusal loading.
  
  
  METHODS
  Class 5 cavities were prepared on the buccal and lingual surfaces of 30 extracted human molars and divided into three groups. The occlusal margins were in enamel, and the cervical margins were in cementum. DBA were applied strictly according to the manufacturers' recommendations, and the preparations were restored with resin composite. Specimens were subjected to 2,000 intermittent occlusal loads and 3,000 thermocycles and then immersed in 0.5% basic fuchsin dye for 72 hours. Four consecutive sections were cut for each specimen and examined under a stereomicroscope. The extent of dye penetration was measured and recorded using a nonparametric scale from 0 to 4.
  
  
  RESULTS
  The Mann-Whitney U test showed a significant difference at cementum margins between One-Step and Dentamed P&B, as well as between Single Bond and Dentamed P&B, and an insignificant difference between Single Bond and One-Step. There was no significant difference in dye penetration at enamel margins between the three DBA.
  
  
  CONCLUSIONS
  Under combined use of occlusal loads and thermocycling, the sealing ability of single-bottle DBA at dentin margins ranged from good for One-Step to moderate for Single Bond to poor for Dentamed P&B. At enamel margins, all materials performed equally well.
authors.affiliation: Department of Oral Rehabilitation, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Ben-Amar
authors.forename: Raphael
authors.initials: R
authors.lastname: Pilo
authors.forename: Eugene
authors.initials: E
authors.lastname: Shapinko
authors.forename: Israel
authors.initials: I
authors.lastname: Lewinstein
mesh_terms: D000269:Adhesives; D017438:Bisphenol A-Glycidyl Methacrylate; D001732:Bite Force; D004396:Coloring Agents; D003188:Composite Resins; D001840:Dental Bonding; D003739:Dental Cementum; D003743:Dental Enamel; D003763:Dental Leakage / Q000175:diagnosis / Q000517:prevention & control*; D003793:Dental Restoration, Permanent; D003799:Dental Stress Analysis; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates; D011897:Random Allocation; D012822:Silicon Dioxide; D018709:Statistics, Nonparametric; D019237:Tooth Cervix; D015040:Zirconium
chemical_list: D000269:Adhesives; C103506:Bis-GMA, BPDM, HEMA dental-bonding resin; D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C083568:Z100 composite resin; C502073:dentamed P&B; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D015040:Zirconium