Fluorescent characteristics of rheumatoid arthritis patients blood lymphocytes.
A fluorescent probe, ABM, aminoderivative of benzanthrone, synthesized in the Department of Organic Chemistry of the Riga Technical University (Latvia), has been successfully used to characterize changes in the structural and functional properties of cell membranes during different pathologies. In the present study the physicochemical properties and the functional activity of the peripheral blood mononuclear cells (lymphocytes-Ly) in patients with rheumatoid arthritis (RA) were studied using the ABM probe. Intensity of the ABM fluorescence in the celi suspension, functional activity of the ly anisotropy of the membranes differ patients with different titres of rheumatoid factor in blood. Patients with seropositive RA had decreased proliferative activity and lower number of iy in blood plasma indicating greater alterations of the immunoregulating processes in these patients as compared to patients with seronegative RA. In the latter the Ly deficiency is compensated to some extent by increased proliferation activity of these cells. The ABM fluorescence intensity correlated not only with membrane anisotropy (r = 0.97, but also with the proliferation activity of the Ly (r = 0.98). The above parameters correlated with the clinical manifestations of the disease. The results indicate that the fluorescent probe ABM is useful for screening the physicochemical status of Ly membranes and the proliferation activity of these cells in RA patients.
Kalniņa
Inta
I
Departmant of Organic Chemistry, Riga Technical University, Riga, Latvia. lulc@lanet.lv
Zvagule
Tija
T
Brūvere
Rūta
R
Meirovics
Imants
I
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood*; D001551:Benz(a)Anthracenes / Q000737:chemistry; D016516:CD4-CD8 Ratio; D049109:Cell Proliferation; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; C477846:3-bromobenzanthrone