Possible effect of 30K proteins in embryonic development of silkworm Bombyx mori.
The silkworm Bombyx mori possesses a 30K protein family of 3x10(4) Da, the biological functions of which have not been fully identified. The relationship between the 30K protein family and the embryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results show that protein spots 1-5 of the 30K protein family, mainly existing in normal strain, are possibly related to embryonic development. The early consumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3 and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development, which may lead to lower hatchability of the temperature sensitive strain. The results suggest that reasonable metabolism of 30K proteins is a prerequisite for the embryo's normal development.
Zhong
Bo-Xiong
BX
Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn
Li
Jian-Ke
JK
Lin
Jian-Rong
JR
Liang
Jian-She
JS
Su
Song-Kun
SK
Xu
Hai-Sheng
HS
Yan
Hai-Yan
HY
Zhang
Ping-Bo
PB
Fujii
Hiroshi
H
D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D019476:Insect Proteins / Q000378:metabolism*; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000378:metabolism*
D019476:Insect Proteins; D020543:Proteome