Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors.
The crystal structure of acetylcholine-binding protein (AChBP) from the mollusk Lymnaea stagnalis is the established model for the ligand binding domains of the ligand-gated ion channel family, which includes nicotinic acetylcholine, 5-hydroxytryptamine (5-HT3), gamma-aminobutyric acid (GABA), types A and C, and glycine receptors. Here we present the crystal structure of a remote homolog, AChBP from Bulinus truncatus, which reveals both the conserved structural scaffold and the sites of variation in this receptor family. These include rigid body movements of loops that are close to the transmembrane interface in the receptors and changes in the intermonomer contacts, which alter the pentamer stability drastically. Structural, pharmacological and mutational analysis of both AChBPs shows how 3 amino acid changes in the binding site contribute to a 5-10-fold difference in affinity for nicotinic ligands. Comparison of these structures will be valuable for improving structure-function studies of ligand-gated ion channel receptors, including signal transduction, homology modeling, and drug design.
Celie
Patrick H N
PH
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Klaassen
Remco V
RV
van Rossum-Fikkert
Sarah E
SE
van Elk
René
R
van Nierop
Pim
P
Smit
August B
AB
Sixma
Titia K
TK
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000737:chemistry; D002151:Calorimetry; D002352:Carrier Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D015195:Drug Design; D017403:In Situ Hybridization; D007477:Ions; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008974:Mollusca; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011950:Receptors, Cholinergic / Q000737:chemistry*; D011978:Receptors, Nicotinic / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013696:Temperature
D002038:Bungarotoxins; D002352:Carrier Proteins; D007477:Ions; D008024:Ligands; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic