title: Quorum-sensing-based toolbox for regulatable transgene and siRNA expression in mammalian cells.
abstract: Technologies for regulated expression of multiple transgenes in mammalian cells have gathered momentum for bioengineering, gene therapy, drug discovery, and gene-function analyses. Capitalizing on recently developed mammalian transgene modalities (QuoRex) derived from Streptomyces coelicolor, we have designed a flexible and highly compatible expression vector set that enables desired transgene/siRNA control in response to the nontoxic butyrolactone SCB1. The construction-kit-like expression portfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional (pBiRex), (iv) oncoretro- and lentiviral transduction, and (v) RNA polymerase II-based siRNA transcription-fine-tuning vectors for straightforward implementation of QuoRex-controlled (trans)gene modulation in mammalian cells.
authors.affiliation: Institute of Chemical- and Bio-Engineering, Swiss Federal Institute of Technology, ETH Hoenggerberg HCI F115, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Weber
authors.forename: Laetitia
authors.initials: L
authors.lastname: Malphettes
authors.forename: Matthias
authors.initials: M
authors.lastname: Rinderknecht
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Schoenmakers
authors.forename: Manuela
authors.initials: M
authors.lastname: Spielmann
authors.forename: Bettina
authors.initials: B
authors.lastname: Keller
authors.forename: Petra
authors.initials: P
authors.lastname: van de Wetering
authors.forename: Cornelia C
authors.initials: CC
authors.lastname: Weber
authors.forename: Martin
authors.initials: M
authors.lastname: Fussenegger
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods; D006224:Cricetinae; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering