title: Encapsulation of osteoblast seeded microcarriers into injectable, photopolymerizable three-dimensional scaffolds based on d,l-lactide and epsilon-caprolactone.
abstract: UMR-106 seeded microcarriers were encapsulated into in situ, photopolymerizable three-dimensional scaffolds based on d,l-lactide and epsilon-caprolactone. UMR-106 and rat bone marrow cells proliferated and differentiated well on the microcarriers. The microcarriers were completely colonized after 14 days in culture. The viscous polymer paste allowed to mix the UMR-106 seeded microcarriers and gelatin (porosigen) properly. After the photopolymerization process, microcarriers and gelatin were evenly distributed throughout the scaffold. Gelatin was leached out within 7 h, and a porous scaffold was obtained. The microcarriers remained in the scaffold even after 7 days which demonstrates that they were well entrapped in the polymer. Increasing the amount of entrapped microcarriers (20-50%) leads to scaffolds with a reduced cross-linking. Hence, the microcarriers leached out. The encapsulated UMR-106 cells did not show pyknotic nuclei which demonstrates that the photopolymerization and handling the viscous polymer/gelatin/microcarrier paste is not detrimental for the cells.
authors.affiliation: Department of Anatomy, Embryology, Histology and Medical Physics, Louis Pasteurlaan 2, Ghent University, B-9000 Ghent, Belgium.
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Declercq
authors.forename: Tomasz L
authors.initials: TL
authors.lastname: Gorski
authors.forename: Séverine P
authors.initials: SP
authors.lastname: Tielens
authors.forename: Etienne H
authors.initials: EH
authors.lastname: Schacht
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Cornelissen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002208:Caproates / Q000008:administration & dosage* / Q000737:chemistry / Q000528:radiation effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004339:Drug Compounding; D007267:Injections; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000528:radiation effects; D007783:Lactones / Q000008:administration & dosage* / Q000737:chemistry / Q000528:radiation effects; D008297:Male; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010775:Photic Stimulation / Q000379:methods; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002208:Caproates; D007783:Lactones; D011108:Polymers; D019344:Lactic Acid; C121056:caprolactone