title: In vitro maturation potential of monocyte-derived dendritic cells is impaired in patients with spinal cord injury: a case-control study.
abstract: OBJECTIVE
  To determine whether the T-cell reactivity and maturation potential of dendritic cells are impaired in subjects with spinal cord injury (SCI).
  
  
  METHODS
  Cross-sectional, case-control study.
  
  
  METHODS
  University hospital in Taiwan.
  
  
  METHODS
  Thirty male SCI subjects, including 14 with paraplegia and 16 with tetraplegia, and 30 age- and sex-matched healthy controls.
  
  
  METHODS
  Not applicable.
  
  
  METHODS
  CD4+ and CD8+ T-cell reactivity was assessed by lymphoproliferative response (LPR) and CD69 expression in response to stimulation with antigens, anti-CD3 monoclonal antibody, and mitogen. Dendritic cell maturation potential was assessed by phenotypic (CD80 and CD83 expression in stimulated monocyte-derived dendritic cells) and functional (the LPR of alloreactive T cells in mixed leukocyte reactions) analysis.
  
  
  RESULTS
  The potential of phenotypic and functional maturation of dendritic cells of subjects with SCI was significantly impaired compared with healthy controls, and that potential was worse for tetraplegic patients than for paraplegic patients. However, no significant difference was found in T-cell responses of CD4+ and CD8+ subsets between subjects with SCI and healthy controls.
  
  
  CONCLUSIONS
  Impaired maturation potential of dendritic cells is a novel defect in innate immunity in people with SCI.
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital and Medical College of National Taiwan University, Taipei, Taiwan.
authors.forename: Sung-Ching
authors.initials: SC
authors.lastname: Pan
authors.forename: Szu-Min
authors.initials: SM
authors.lastname: Hsieh
authors.forename: Yen-Ho
authors.initials: YH
authors.lastname: Wang
authors.forename: Bor-Luen
authors.initials: BL
authors.lastname: Chiang
authors.forename: Tien-Shang
authors.initials: TS
authors.lastname: Huang
authors.forename: Shan-Chwen
authors.initials: SC
authors.lastname: Chang
mesh_terms: D000328:Adult; D015703:Antigens, CD; D018122:B7-1 Antigen / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D013119:Spinal Cord Injuries / Q000276:immunology* / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D015704:CD4 Antigens; D016827:CD8 Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen