title: IgE production after four routes of injections of Japanese cedar pollen allergen without adjuvant: crucial role of resident cells at intraperitoneal or intranasal injection site in the production of specific IgE toward the allergen.
abstract: The production of specific IgE antibodies directed toward cedar pollen correlates well with the onset of allergic rhinitis; but the mechanisms of allergen recognition as nonself and Ig class switch to IgE by the immune system are still not fully understood. In the present study, we injected cedar pollen into mice through 4 different routes (intranasal (i.n.), intraperitoneal (i.p.), intravenous (i.v.), and subcutaneous (s.c.)) without adjuvant 1 to 3 times, and determined time-dependent changes in the total and specific serum IgE levels compared with those in the serum levels of other isotype Igs. After an i.p. or i.n. injection of allergen into the mice, they produced a 1.5-to 1.7-fold increase in total IgE, but none in IgG, IgM, or IgA antibodies in their serum, whereas an i.v. or s.c. injection of allergen was inactive as an inducer of total IgE antibodies. Upon a 2nd (s.c.) injection of the allergen into the i.p. or i.n. sensitized mice, a large amount of allergen-specific IgE antibodies was found in the serum. In the case of i.v. or s.c. sensitized mice, however, they produced total, but not specific, IgE antibodies; and a 3rd (s.c.) injection of the allergen resulted in a large amount of specific IgE antibodies in the serum. These results imply that resident cells at the i.p. or i.n. injection site may play a crucial role in the efficient production of total and specific IgE antibodies toward the allergen.
authors.affiliation: Department of Physiology, Osaka Medical College, Daigaku-machi, Takatsuki, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Sakurai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takenaka
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yoneda
authors.forename: Junko
authors.initials: J
authors.lastname: Tashiro-Yamaji
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Koutetsu
authors.initials: K
authors.lastname: Lee
authors.forename: Satoko
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Masako
authors.initials: M
authors.lastname: Miyoshi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kubota
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Yoshida
mesh_terms: D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology*; D000818:Animals; D028203:Cedrus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007564:Japan; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000276:immunology*
chemical_list: D000485:Allergens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E