Effects of combined T- and B-cell deficiency on murine ischemia reperfusion injury.
B and T cells have been implicated in the pathogenesis of renal ischemia reperfusion injury (IRI); however, it is unknown if B and T cells interact in early injury responses, as seen in adaptive immune responses. Recent evidence has shown that B-cell deficient and T-cell deficient mice are partially protected from renal IRI. Renal IRI was induced in recombinase activating gene (RAG)-1 deficient mice, which lack both B and T cells. RAG-1 deficient mice from two different background strains were not protected from renal IRI. Adoptive transfer of either B or T cells into RAG-1 deficient mice led to a significant protection of renal injury, which was independent of effects on neutrophil trafficking. Neutrophil depletion in RAG-1 deficient mice did not protect from IRI. While deficiency of either B or T cells reduced IRI, combined lack of both is not protective. These results demonstrate that complex interactions between B and T cells are likely occurring in kidney IRI.
Burne-Taney
Melissa J
MJ
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yokota-Ikeda
Naoko
N
Rabb
Hamid
H
D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D007511:Ischemia / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D007668:Kidney / Q000098:blood supply* / Q000276:immunology / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D015427:Reperfusion Injury / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation
D018398:Homeodomain Proteins; C064658:RAG-1 protein