Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.
We have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside (GalNAc alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent-resistant membranes (DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post-Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM association characteristics of apical proteins. Sulfatides with long chain-hydroxylated fatty acids, which were also enriched in DRMs, were identified as high-affinity ligands for galectin-4. Together, our data propose that interaction between galectin-4 and sulfatides plays a functional role in the clustering of lipid rafts for apical delivery.
Delacour
Delphine
D
Unité INSERM 560, 59045 Lille Cedex, France.
Gouyer
Valérie
V
Zanetta
Jean-Pierre
JP
Drobecq
Hervé
H
Leteurtre
Emmanuelle
E
Grard
Georges
G
Moreau-Hannedouche
Odile
O
Maes
Emmanuel
E
Pons
Alexandre
A
André
Sabine
S
Le Bivic
André
A
Gabius
Hans Joachim
HJ
Manninen
Aki
A
Simons
Kai
K
Huet
Guillemette
G
D002451:Cell Compartmentation / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D020895:Enterocytes / Q000378:metabolism* / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D005690:Galactose / Q000031:analogs & derivatives* / Q000494:pharmacology; D037541:Galectin 4 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D021962:Membrane Microdomains / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D034622:RNA Interference; D013433:Sulfoglycosphingolipids / Q000737:chemistry / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism / Q000648:ultrastructure
C411946:1-benzyl-2-acetamido-2-galactopyranoside; D003902:Detergents; D004791:Enzyme Inhibitors; D037541:Galectin 4; D013433:Sulfoglycosphingolipids; D005690:Galactose