title: Mandibular distraction osteogenesis: a rabbit model using a novel experimental design.
abstract: OBJECTIVE
  Distraction osteogenesis (DO) is a surgical procedure that targets bone regeneration and elongation, currently used in the treatment of many craniofacial deformities. The quest for optimization of DO clinical parameters has led to the development of a variety of animal models. Our study aims to establish a rabbit animal model of mandibular DO, in which the control osteotomy and distraction device are placed on the opposite hemimandible from the one being distracted, within the same animal host. Furthermore, we propose to histologically characterize the different stages or distraction and consolidation in the same animal model.
  
  
  METHODS
  Twenty-five rabbits underwent mandibular osteotomies and bilateral placement of distraction devices. After a latency of 3 days, the distraction device was activated on one side of each animal at a rate of 0.5 mm/12 hours for 7 days, while the other side remained inactive (control). This was followed by a consolidation period of 14 days. Five animals per time-point were killed on days 3, 7, 10, 17, and 24.
  
  
  RESULTS
  Gross tissue analysis showed a 7-mm callus formation at the distracted side and a well-healed osteotomy in the non-distracted side. Clinically, a unilateral Class III malocclusion occurred in the distracted side. Histology at each time-point shows new bone formation and orientation of the bony spicules along the axis of the mechanical strain.
  
  
  CONCLUSIONS
  We have established and characterized an animal model of mandibular DO that outlines valid biologic controls and provides thorough monitoring of the DO process.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tufts University School of Dental Medicine, Boston, MA 02111, USA.
authors.forename: Maisa O
authors.initials: MO
authors.lastname: Al-Sebaei
authors.forename: Eleni
authors.initials: E
authors.lastname: Gagari
authors.forename: Maria
authors.initials: M
authors.lastname: Papageorge
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D016267:External Fixators; D017102:Fracture Healing / Q000502:physiology*; D008297:Male; D008334:Mandible / Q000601:surgery*; D023421:Models, Animal; D019857:Osteogenesis, Distraction / Q000295:instrumentation / Q000379:methods*; D010027:Osteotomy / Q000295:instrumentation / Q000379:methods; D011817:Rabbits; D012107:Research Design; D013997:Time Factors