Isolation of Escherichia coli O5 :H-, possessing genes for Shiga toxin 1, intimin-beta and enterohaemolysin, from an intestinal biopsy from an adult case of bloody diarrhoea: evidence for two distinct O5 :H- pathotypes.
Two typical coliforms from an intestinal biopsy from an adult case of bloody diarrhoea carried genes encoding intimin-beta, stx(1) and ehxA, and produced verocytotoxin 1 and enterohaemolysin in culture. Both were biochemically typical Escherichia coli O5 :H(-), apart from producing urease. Such O5 isolates represent a human pathogenic E. coli lineage.
McLean
Catriona
C
Department of Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia 2National Escherichia coli Reference Laboratory, Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia 3NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, New South Wales 2570, Australia.
Bettelheim
Karl A
KA
Department of Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia 2National Escherichia coli Reference Laboratory, Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia 3NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, New South Wales 2570, Australia.
Kuzevski
Alexander
A
Department of Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia 2National Escherichia coli Reference Laboratory, Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia 3NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, New South Wales 2570, Australia.
Falconer
Linda
L
Department of Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia 2National Escherichia coli Reference Laboratory, Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia 3NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, New South Wales 2570, Australia.
Djordjevic
Steven P
SP
Department of Pathology, Alfred Hospital, Prahran, Victoria 3181, Australia 2National Escherichia coli Reference Laboratory, Microbiological Diagnostic Unit, Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia 3NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Microbiology and Immunology Section, New South Wales 2570, Australia.
D018829:Adhesins, Bacterial / Q000235:genetics; D001706:Biopsy; D004403:Dysentery / Q000382:microbiology* / Q000473:pathology; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D029968:Escherichia coli Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D022622:Shiga Toxin 1 / Q000032:analysis / Q000235:genetics
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022622:Shiga Toxin 1; C066219:enterohemolysin; C073819:eaeA protein, E coli