Cloning and characterization of a gene for an LRR receptor-like protein kinase associated with cotton fiber development.
Cotton fiber is an ideal model for studying plant cell elongation and cell wall biogenesis, but the genes that are critical for the regulation of fiber development are largely unknown. We report here the cloning and characterization of a receptor-like kinase gene (designated GhRLK1), expression of which is induced during the period of active secondary wall synthesis in the cotton fiber cells. We demonstrate that GhRLK1 is located in the plasma membrane and shows dual specificity as both a serine/threonine kinase and a tyrosine kinase. Our results suggest a possible role of GhRLK1 in the signal transduction pathway that is involved in the induction and maintenance of active secondary wall formation during fiber development.
Li
Yuan-Li
YL
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, China.
Sun
Jie
J
Xia
Gui-Xian
GX
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D032561:Cotton Fiber; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000201:enzymology
D018076:DNA, Complementary; D011994:Recombinant Proteins; D011494:Protein Kinases