[Detection and quantification of nucleic acids in infections diseases: utility and limitations].
The amplification of nucleic acids is often used for the diagnosis and the follow-up of infectious diseases. The interpretation of PCR results varies according to the pathogens detected, the site of infection and the clinical presentation. PCR tests might be the reference method or only an help for the diagnosis. With the development of antiviral treatments quantitative PCR tests are now essential for the evaluation of treatment efficacy. A discussion with the laboratory is important for the correct interpretation of PCR results.
Yerly
Sabine
S
Laboratoire central de virologie Service des maladies infectieuses Département de médecine interne Hôpitaux universitaires de Genève. sabine.yerly@hcuge.ch
Kaiser
Laurent
L
Ninet
Béatrice
B
D003141:Communicable Diseases / Q000175:diagnosis* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D009696:Nucleic Acids / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004279:DNA, Viral; D009696:Nucleic Acids