title: [Immunogenicity of bovine jugular vein conduits treated with different cross-linking methods].
abstract: OBJECTIVE
  To evaluate the immunogenicity of bovine jugular vein conduits (BJVCs) treated with different cross-linking methods.
  
  
  METHODS
  The BJVCs were treated with glutaradehyde (GA), dye-mediated photooxidation (DMP) and polyepoxy compound (PC) (n = 10). The tissue homogenates obtained from BJVCs treated with PC, GA, DMP, and fresh BJVCs, were mixed with the complete Freand adjavant to form the emulsive antigen, which were used to immunize rabbits correspondently. The antibody concentrations to BJVCs in those rabbits' serum were measured by counter double immuno diffusion. The immunologic responses to the BJVCs in different groups were measured with Western blotting.
  
  
  RESULTS
  The positive bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with antigens of fresh BJVCs, but no bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with those antigens of the BJVCs treated with GA, DMP, and PC in Western blotting.
  
  
  CONCLUSIONS
  The immunogenicity of BJVCs treated with PC, DMP, and GA can be reduced significantly and meet the clinical standard.
authors.affiliation: Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Hu
authors.forename: Zhong-shi
authors.initials: ZS
authors.lastname: Wu
authors.forename: Tei-hui
authors.initials: TH
authors.lastname: Hu
authors.forename: You-hua
authors.initials: YH
authors.lastname: Deng
authors.forename: Yao-guang
authors.initials: YG
authors.lastname: Feng
authors.forename: Zhi-bing
authors.initials: ZB
authors.lastname: Jiang
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002417:Cattle; D003432:Cross-Linking Reagents; D005976:Glutaral / Q000494:pharmacology; D007601:Jugular Veins / Q000276:immunology*; D019736:Prostheses and Implants; D011817:Rabbits; D011897:Random Allocation
chemical_list: D003432:Cross-Linking Reagents; D005976:Glutaral