Giardia lamblia: expression of alkaline phosphatase activity in infected rat intestine.
Alkaline phosphatase (IAP) is a marker of intestinal microvillus membrane. Changes in IAP activity have been studied as a function of Giardia lamblia (G. lamblia) infection using rat as the experimental model. At day 11 and 15 post-infection, enzyme activity was reduced (p<0.01) compared to controls. The enzyme levels were essentially similar to control values by day 30 post-infection. Analysis of the enzyme activity in cell fractions across crypt-villus axis revealed a marked decrease in enzyme activity in the villus tip and mid villus regions but a considerable increase (p<0.01) in enzyme activity in the crypt base of 11 day post-infected animals compared to that in controls. The observed changes in IAP activity were confirmed by assaying the enzyme activity in acrylamide gels using bromo-chloro-indolyl phosphate staining under non-denaturing conditions. These findings indicate differential changes across the crypt-villus axis, but impaired alkaline phosphatase levels in G. lamblia infected rat intestine.
Mahmood
Safrun
S
Department of Biochemistry, Panjab University, Chandigarh 160012, India. akhtarmah@yahoo.com
Kaur
Kamaljit
K
Mittal
Nidhi
N
Mahmood
Akhtar
A
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D016829:Giardia lamblia / Q000502:physiology*; D005873:Giardiasis / Q000201:enzymology*; D007422:Intestines / Q000201:enzymology* / Q000469:parasitology; D008297:Male; D008871:Microvilli / Q000201:enzymology / Q000469:parasitology; D051381:Rats; D017208:Rats, Wistar
D000469:Alkaline Phosphatase