In situ hybridization localization of TRH precursor and TRH receptor mRNAs in the brain and pituitary of Xenopus laevis.
We examined the distribution of the mRNAs encoding proTRH and the three TRH receptor subtypes (xTRHR1, xTRHR2, and xTRHR3) in the Xenopus laevis CNS and pituitary. A positive correlation was generally observed between the expression patterns of proTRH and xTRHR mRNAs. xTRHRs were widely expressed in the telencephalon and diencephalon, where two or even three xTRHR mRNAs were often simultaneously observed within the same brain structures. In the pituitary, xTRHR2 was selectively expressed in the distal lobe, and xTRHR3 was found exclusively in the intermediate lobe of white background-adapted animals, indicating that, in amphibians, the effect of TRH on alpha-melanotropin (alpha-MSH) secretion from melanotrope cells is mediated through the novel receptor subtype xTRHR3.
Galas
L
L
European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.
Bidaud
I
I
Bulant
M
M
Jenks
B G
BG
Ouwens
D T W M
DT
Jégou
S
S
Ladram
A
A
Roubos
E W
EW
Nicolas
P
P
Tonon
M C
MC
Vaudry
H
H
D000818:Animals; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D010902:Pituitary Gland / Q000737:chemistry* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000235:genetics* / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism*; D014982:Xenopus laevis
D011498:Protein Precursors; D012333:RNA, Messenger; D018025:Receptors, Thyrotropin-Releasing Hormone; D029867:Xenopus Proteins; D013973:Thyrotropin-Releasing Hormone; C040476:thyrotropin-releasing hormone, Gly; D011761:Pyrrolidonecarboxylic Acid