title: Identification and characterization of a selenium-dependent glutathione peroxidase in Setaria cervi.
abstract: Setaria cervi a bovine filarial parasite secretes selenium glutathione peroxidase during in vitro cultivation. A significant amount of enzyme activity was detected in the somatic extract of different developmental stages of the parasite. Among different stages, microfilariae showed a higher level of selenium glutathione peroxidase activity followed by males then females. However, when the activity was compared in excretory secretory products of these stages males showed higher activity than microfilariae and female worms. The enzyme was purified from female somatic extract using a combination of glutathione agarose and gel filtration chromatography, which migrated as a single band of molecular mass approximately = 20 kDa. Selenium content of purified enzyme was estimated by atomic absorption spectroscopy and found to be 3.5 ng selenium/microg of protein. Further, inhibition of enzyme activity by potassium cyanide suggested the presence of selenium at the active site of enzyme. This is the first report of identification of selenium glutathione peroxidase from any filarial parasite.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
authors.forename: Anchal
authors.initials: A
authors.lastname: Singh
authors.forename: Sushma
authors.initials: S
authors.lastname: Rathaur
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005979:Glutathione Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D017182:Setaria Nematode / Q000201:enzymology*
chemical_list: D005979:Glutathione Peroxidase; D012643:Selenium