title: Manufacturing of nanochannels with controlled dimensions using protease nanolithography.
abstract: The feasibility of creating nanometer scale depressions in biological substrates using active enzymes delivered with scanning probe microscopes has been previously demonstrated by us and other groups. Here we present a comprehensive study revealing the dependence of channels dimensions on the parameters of the "writing" process and provide a simple way to precisely control their dimensions. Such nanochannels may be used in nanofluidic biochip applications.
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
authors.forename: Levi A
authors.initials: LA
authors.lastname: Gheber
mesh_terms: D000327:Adsorption; D003460:Crystallization / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000648:ultrastructure; D008420:Manufactured Materials / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010781:Photography / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D014357:Trypsin