[Development of high performance liquid chromatographic method using monolithic column for the determination of urinary nucleosides].
Modified nucleosides excreted in urine have been studied as possible bio-markers for malignant tumors. Reversed-phase high performance liquid chromatography (RP-HPLC) is one of the most popular analytical tools. However, its analysis time is longer than desired. The analytical method for urinary nucleosides has been developed using a monolithic column and a linear gradient elution of 25 mmol/L KH2PO4 solution (pH 4.55) and 60% methanol in water with UV detection at 260 nm. Twelve urinary nucleosides were completely separated. The monolithic column presented similar analytical characteristics to reversed-phase column. The time of the analysis is only 23 min, in general much shorter than the methods using a packed RP-HPLC. The method developed is sensitive, reliable and suitable for clinical applications.
Zhao
Xinjie
X
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China.
Zheng
Yufang
Y
Zhang
Pudun
P
Kong
Hongwei
H
Xu
Guowang
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D009705:Nucleosides / Q000302:isolation & purification* / Q000652:urine; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D009705:Nucleosides