Comparison of cytocompatibility of zein film with other biomaterials and its degradability in vitro.
Cytocompatibility of particle zein (Pzein) and film zein (Fzein) was evaluated and compared with polyhydroxybutyrate (PHB), its copolymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), polylactic acid (PLA), and collagen, using HL-7702 cells, in terms of cell attachment rate within 3 h, and cell viabilities at 3 and 6 days determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. The zein degradation test was carried out using collagenase and trypsin, and the degradation product was added to the culture medium at different concentrations in order to examine the concentration-dependent cytotoxic effect.


RESULTS
The adhesion rate of the HL-7702 cells on both Pzein and Fzein was higher than that on collagen film. Cell viabilities were higher on both Pzein and Fzein than on films of PLA, PHB, PHBV, and collagen from fish skin. Zein can be degraded by both trypsin and collagenase, and the degradation product can enhance cell viability within a certain range of concentrations.
Sun
Qing-Shen
QS
School of Life Science and Biotechnology, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, China.
Dong
Jian
J
Lin
Zhi-Xin
ZX
Yang
Bin
B
Wang
Jin-Ye
JY
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D017364:Collagenases / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D000091402:Prohibitins; D014357:Trypsin / Q000737:chemistry; D015028:Zein / Q000737:chemistry* / Q000494:pharmacology*; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D003094:Collagen; D017364:Collagenases; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D000091402:Prohibitins; D014357:Trypsin; D015028:Zein; C000717328:PHB protein, human; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C033616:poly(lactide)