[Detecting DNA damage of cell in rats using comet assay after tetramine poisoned].
OBJECTIVE
To study the damage of DNA in lymphocytes, brain cells and cardiac muscle cells of rats induced by different dose of tetramine and to speculate the toxicant mechanism of tetramine.


METHODS
The rat were poisoned by Tetramine, which was taken in by mouth. The rat poisoning models were used by 0.2, 0.1, 0.05, 0.01 mg x kg(-1) Tetramine, and comparison model was made by NS. Lymphocytes and brain cells and cardiac muscle cells of rats were separatd and collected form experimentation rat. DNA damages of cells which were exposed to different doses of tetramine were detected using the single cell gel electrophorresis (SCGE) or comet assay.


RESULTS
DNA damages have been observed in lymphocytes, brain cells and cardiac muscle cells of rats which exposed form 0.01mg x kg(-1) doses of tetramine to 0.2mg x kg(-1) doses of tetramine. The test groups are very significantly statistical different to the control group (P<0.01).


CONCLUSIONS
It is assumed that DNA damages of cells might be one of the toxicant mechanism of tetramine.
Zhu
Chuan-hong
CH
Department of Medicolegal, Tongii Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
Liu
Yan
Y
Deng
Li-bin
LB
D000818:Animals; D001921:Brain / Q000473:pathology*; D001952:Bridged-Ring Compounds / Q000506:poisoning*; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel / Q000379:methods; D008214:Lymphocytes / Q000187:drug effects; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001952:Bridged-Ring Compounds; C010349:tetramethylenedisulfotetramine