title: The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules.
abstract: The protooncogenic protein c-Cbl is known to regulate the actin cytoskeleton. In this study, we present results indicating that c-Cbl can also regulate the microtubular network. We have shown that c-Cbl binds to tubulin and microtubules through its tyrosine kinase binding (TKB) domain. However, the character of the interactions described in this report is novel, since the G306E mutation, which disrupts the ability of c-Cbl's TKB to bind to tyrosine-phosphorylated proteins, does not affect the observed interaction between c-Cbl and microtubules. Furthermore, overexpression of c-Cbl in human pulmonary artery endothelial cells and COS-7 cells leads to microtubule stabilization. We demonstrate that this effect of c-Cbl is mediated by TKB, and, like c-Cbl binding to microtubules, is independent of the ability of TKB to bind to tyrosine-phosphorylated proteins. Finally, we have shown that c-Cbl directly polymerizes microtubules in vitro, and that TKB is necessary and sufficient for this effect of c-Cbl. In this last phenomenon, as well as in the previous ones, the effect of TKB is not sensitive to the inactivating G306E mutation. Overall, the results presented in this report suggest a novel function for c-Cbl-microtubule binding and stabilization.
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, 3400 N. Broad Street, Philadelphia, PA 19140, USA.
authors.forename: Anjali M
authors.initials: AM
authors.lastname: Teckchandani
authors.forename: Anna A
authors.initials: AA
authors.lastname: Birukova
authors.forename: Krisztina
authors.initials: K
authors.lastname: Tar
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Verin
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Tsygankov
mesh_terms: D000107:Acetylation; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002465:Cell Movement; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D042783:Endothelial Cells / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D008168:Lung / Q000166:cytology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D050721:Proto-Oncogene Proteins c-cbl; D014404:Tubulin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D011518:Proto-Oncogene Proteins; D014404:Tubulin; D050721:Proto-Oncogene Proteins c-cbl; D044767:Ubiquitin-Protein Ligases; C067419:CBL protein, human