title: Acute histologic effects of extraesophageal reflux on vocal fold healing.
abstract: This study evaluates how extraesophageal reflux affects membranous vocal fold healing in a canine model. We created membranous vocal fold injuries in the animals and randomly assigned them to topical application of acid and pepsin at pH 2 or pH 6 or of normal saline solution every other day for 12 days. The experimental vocal folds were compared to uninjured, control vocal folds from animals painlessly sacrificed for other reasons. Hematoxylin and eosin, fibronectin, and procollagen I staining were performed for histologic analysis. The injured specimens had three times greater cellular infiltrate (p < or = .001, analysis of variance) and twice as much fibronectin and procollagen I (p < or = .001, analysis of variance) as did the specimens from the control animals. No significant differences or trends were identified for cellular infiltrate, fibronectin, or procollagen I within the injured groups (p > .05, Bonferroni t-test). Acute wound healing did not appear to be influenced by the presence of acid and pepsin at pH 2 or 6 as compared to saline solution.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Vanderbilt University Medical Center Nashville, Tennessee, USA.
authors.forename: Seth M
authors.initials: SM
authors.lastname: Cohen
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: C Gaelyn
authors.initials: CG
authors.lastname: Garrett
authors.forename: Mark S
authors.initials: MS
authors.lastname: Courey
mesh_terms: D000143:Acids / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005764:Gastroesophageal Reflux / Q000378:metabolism / Q000473:pathology*; D005765:Gastrointestinal Agents / Q000008:administration & dosage; D006863:Hydrogen-Ion Concentration; D007828:Laryngoscopy; D010434:Pepsin A / Q000008:administration & dosage; D011347:Procollagen / Q000378:metabolism; D011897:Random Allocation; D014827:Vocal Cords / Q000293:injuries* / Q000378:metabolism / Q000473:pathology*; D014945:Wound Healing
chemical_list: D000143:Acids; D005353:Fibronectins; D005765:Gastrointestinal Agents; D011347:Procollagen; D010434:Pepsin A