title: Seeding of recipient bone marrow cells reduces neointimal hyperplasia of de-endothelialized rat aortic allograft.
abstract: Transplant vasculopathy is a leading cause of graft failure and a major contributor to the lack of success with small caliber vascular allografts. In this study we evaluate techniques of bone marrow cell seeding on small caliber vessels and assess the impact of this tactic on neointimal hyperplasia in de-endothelialized rat aortic allografts. In a preliminary study, bone marrow cells from Lewis rats were seeded onto the chemically de-endothelialized luminal surface of the abdominal aorta of WKY rats - with or without fibrin glue. In the allograft transplantation model, de-endothelialized fresh aortic allografts of WKY rats were orthotopically transplanted into Lewis recipients either directly (n = 6) or after recipient bone marrow cell seeding (n = 6). Histological evaluation was performed at 28 days. Bone marrow cells were able to adhere to the de-endothelialized aortic wall owing to the use of fibrin glue, but were unable to do so without fibrin glue. In the de-endothelialized allograft transplantation model, recipient bone marrow seeding led to a significant reduction of the ratio of intimal to medial area (0.40 +/- 0.08 versus 0.79 +/- 0.08, P = 0.0077). Some of the seeded cells remained in the intima for 4 weeks and some infiltrated the media, expressing CD31 or alpha-SMA. The results suggest that recipient bone marrow cell seeding on de-endothelialized aortic allograft is feasible with the use of fibrin glue and that this technique reduces neointimal hyperplasia of the graft.
authors.affiliation: Department of Cardiothoracic Surgery, University of Tokyo Graduate School of Medicine, Hongo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitamura
authors.forename: Masataka
authors.initials: M
authors.lastname: Sata
authors.forename: Noboru
authors.initials: N
authors.lastname: Motomura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takamoto
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001011:Aorta / Q000637:transplantation*; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D004730:Endothelium, Vascular / Q000473:pathology; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D006965:Hyperplasia / Q000517:prevention & control; D008297:Male; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D011921:Rats, Inbred WKY; D014184:Transplantation, Homologous
chemical_list: D000199:Actins; D015718:Fibrin Tissue Adhesive; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C498862:Acta2 protein, rat