Evaluation of modifying collagen matrix with RGD peptide through periodate oxidation.
The aim of the study is to evaluate the effect of modifying collagen matrices with Arg-Gly-Asp (RGD) peptide through periodate oxidation. The collagen matrices were modified with RGD peptide, by periodate activation. The modified collagen matrices and unmodified matrices were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and electron spectroscopy for chemical analysis (ESCA). Mesenchymal stem cells (MSCs) were used to evaluate the cell compatibility of collagen matrices. In terms of cell growth, the MSCs attached much better on the modified matrix than on the unmodified one. But there was no significant difference between two groups regarding the MSC proliferation. Compared to the unmodified matrices, the mechanical strength of the modified matrix decreased sharply, and its 3D structure was destroyed. Introducing specific RGD receptor-mediated adhesion sites on matrices obviously enhanced the MSC adhesion on collagen matrices, but the coupled method of periodate oxidation would likely result in the declination of the mechanical strength of the matrix, as well as the destruction of the matrix structure. This would affect the cell growth on the matrix, and decrease the histocompatibility of the matrices.
Zhang
Lihai
L
Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, People's Republic of China. zhanglihai74@sohu.com
Hum
Min
M
Wang
Meng
M
Li
Yanfeng
Y
Chen
Hua
H
Chu
Chengbin
C
Jiang
Hua
H
D000042:Absorption; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials; D002151:Calorimetry; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D005269:Femur / Q000473:pathology; D008648:Mesoderm / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000494:pharmacology*; D010100:Oxygen / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength; D013997:Time Factors; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D001672:Biocompatible Materials; D009842:Oligopeptides; D010455:Peptides; D014867:Water; D010504:Periodic Acid; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen; C009288:metaperiodate; D010100:Oxygen