title: High-sensitivity ion mobility spectrometry/mass spectrometry using electrodynamic ion funnel interfaces.
abstract: The utility of ion mobility spectrometry (IMS) for separation of mixtures and structural characterization of ions has been demonstrated extensively, including in biological and nanoscience contexts. A major attraction of IMS is its speed, several orders of magnitude greater than that of condensed-phase separations. Nonetheless, IMS combined with mass spectrometry (MS) has remained a niche technique, substantially because of limited sensitivity resulting from ion losses at the IMS-MS junction. We have developed a new electrospray ionization (ESI)-IMS-QTOF MS instrument that incorporates electrodynamic ion funnels at both front ESI-IMS and rear IMS-QTOF interfaces. The front funnel is of the novel "hourglass" design that efficiently accumulates ions and pulses them into the IMS drift tube. Even for drift tubes of 2-m length, ion transmission through IMS and on to QTOF is essentially lossless across the range of ion masses relevant to most applications. The rf ion focusing at the IMS terminus does not degrade IMS resolving power, which exceeds 100 (for singly charged ions) and is close to the theoretical limit. The overall sensitivity of the present ESI-IMS-MS system is comparable to that of commercial ESI-MS, which should make IMS-MS suitable for analyses of complex mixtures with ultrahigh sensitivity and exceptional throughput.
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
authors.forename: Keqi
authors.initials: K
authors.lastname: Tang
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Shvartsburg
authors.forename: Hak-No
authors.initials: HN
authors.lastname: Lee
authors.forename: David C
authors.initials: DC
authors.lastname: Prior
authors.forename: Michael A
authors.initials: MA
authors.lastname: Buschbach
authors.forename: Fumin
authors.initials: F
authors.lastname: Li
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Tolmachev
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D007328:Insulin / Q000032:analysis / Q000737:chemistry; D017136:Ion Transport; D017736:Ion-Selective Electrodes; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D007328:Insulin; D010455:Peptides