[Expression and immunity reaction of a novel gene OmpL17 of the strong virulent L. interrogans serovar Lai in China].
This study was conducted to potentiate the expression of outer membrane protein OmpL17 of the strong virulent L. interrogans serovar Lai and investigate its immunogenicity in rabbits. The OmpL17 was cloned into prokaryotic expression vector pGEX-1lambdaT. The recombination expression plasmid pGEX-OmpL17 was transformed into E. Coli JM109. The GST fused protein GST-OmpL17 was expressed after induction by IPTG, then GST-tag was by thrombin and purified using Bulk GST purification Modules. SDS-PAGE and Western blotting analysis indicated that the molecular weight of GST-OmpL17 and OmpL17 was about 54 KDa and 28 KDa respectively. The outer membrane protein OmpL17 was subcutaneously injected into rabbits and high titre anti-OmpL17 antibody was obtained (1:4896) which could conjugate specifical with OmpL17. In conclusion, OmpL17 and specifical anti-OmpL17 antibody were obtained, which provided an experimental basis for researching pathogenic effect and immunity functions of OmpL17.
Zhu
Qingping
Q
Research Unit of Infection and Immunity, West China Center of Medical Sciences, Sichuan University, Chengdu 610041, China.
Zhao
Jilin
J
Bao
Lang
L
Zhang
Huidong
H
Zhao
Mingcai
M
Li
Guang
G
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D006801:Humans; D007921:Leptospira interrogans / Q000235:genetics* / Q000276:immunology; D018272:Porins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C100900:OmpL protein, bacteria; D018272:Porins; D011994:Recombinant Proteins; C081904:ompL1 protein, Leptospira