title: Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts.
abstract: To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-alpha, MHC-DR-beta, MHC-DR-associated protein CD74, tissue factor pathway inhibitor-2, and neuroserpin were much higher in MSC than in fibroblasts, even in the presence of large interindividual variations. Those of adrenomedullin, apolipoprotein D, C-type lectin superfamily member-2, collagen type XV alpha1, CUG triplet repeat RNA-binding protein, matrix metalloproteinase-1, protein tyrosine kinase-7, and Sam68-like phosphotyrosine protein/T-STAR were lower in MSC than in fibroblasts. FACS analysis showed that cell surface expression of MHC-DR was also higher in MSC than in fibroblasts. MHC-DR expression decreased after osteogenic differentiation, whereas the expression of adrenomedullin-a potent stimulator of osteoblast activity-along with collagen XV alpha1 and apolipoprotein D increased after osteogenic differentiation. The marker genes identified in this study should be useful for characterization of MSC both in basic and clinical studies.
authors.affiliation: Department of Dental and Medical Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ishii
authors.forename: Chika
authors.initials: C
authors.lastname: Koike
authors.forename: Akira
authors.initials: A
authors.lastname: Igarashi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yamanaka
authors.forename: Haiou
authors.initials: H
authors.lastname: Pan
authors.forename: Yukihito
authors.initials: Y
authors.lastname: Higashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Masaru
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kamata
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Iwata
authors.forename: Takehiro
authors.initials: T
authors.lastname: Matsubara
authors.forename: Kozo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tsuji
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers