title: Plp gene regulation in the developing murine optic nerve: correlation with oligodendroglial process alignment along the axons.
abstract: The factors regulating the expression and splicing of the major myelin gene, proteolipid protein (Plp), are unclear. The gene encodes two splice variants, Plp and Dm20. During active myelination, transcription of the Plp gene is markedly upregulated and the splice variant ratio becomes Plp-mRNA dominant. We hypothesised that these aspects of Plp gene regulation are linked to overt axonal contact. Using the developing optic nerve of mice, we demonstrate that alignment of oligodendroglial processes with the axon correlates with both the expression of Plp-mRNA and the transcriptional upregulation of the gene. We test the above hypothesis more extensively in a subsequent study.
authors.affiliation: Applied Neurobiology Group, Insititute of Comparative Medicine, Division of Cell Sciences, Glasgow University Veterinary School, Glasgow, UK. C.Thomson@vet.gla.ac.uk
authors.forename: C E
authors.initials: CE
authors.lastname: Thomson
authors.forename: D A
authors.initials: DA
authors.lastname: Vouyiouklis
authors.forename: J A
authors.initials: JA
authors.lastname: Barrie
authors.forename: K N
authors.initials: KN
authors.lastname: Wease
authors.forename: P
authors.initials: P
authors.lastname: Montague
mesh_terms: D000375:Aging / Q000378:metabolism; D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018991:Myelin Proteolipid Protein / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D009836:Oligodendroglia / Q000378:metabolism* / Q000648:ultrastructure; D009900:Optic Nerve / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C047516:Plp1 protein, mouse; D012333:RNA, Messenger