title: White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods.
abstract: OBJECTIVE
  White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.
  
  
  METHODS
  As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).
  
  
  RESULTS
  Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.
  
  
  CONCLUSIONS
  The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.
authors.affiliation: Sanquin Blood Bank North-west Region, 100 AC Amsterdam, the Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dijkstra-Tiekstra
authors.forename: C E
authors.initials: CE
authors.lastname: van der Schoot
authors.forename: R N I
authors.initials: RN
authors.lastname: Pietersz
authors.forename: H W
authors.initials: HW
authors.lastname: Reesink
mesh_terms: D004247:DNA / Q000032:analysis; D005374:Filtration; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods* / Q000592:standards
chemical_list: D019007:P-Selectin; D004247:DNA