Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter.
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.
Im
Seung-Soon
SS
Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea.
Kim
Jae-Woo
JW
Kim
Tae-Hyun
TH
Song
Xian-Li
XL
Kim
So-Youn
SY
Kim
Ha-il
HI
Ahn
Yong-Ho
YH
D000818:Animals; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D051273:Glucose Transporter Type 2; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000096:biosynthesis; D020218:Response Elements; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D047493:PPAR alpha; D047495:PPAR gamma; D020033:Protein Isoforms; D045162:Thiazolidinediones; D000077154:Rosiglitazone