title: Eccrine-centred distribution of human papillomavirus 63 infection in the epidermis of the plantar skin.
abstract: BACKGROUND
  The primary target cell of human papillomaviruses (HPVs) is an unsettled issue. Recent studies have suggested that the hair follicle is an important candidate as the reservoir of certain HPV types. However, little is known about the cells which serve as the target or the reservoir of HPVs in nonhairy palmoplantar skin.
  
  
  OBJECTIVE
  To investigate whether the eccrine sweat gland, the only skin appendage in nonhairy palmoplantar skin, also serves as the target or the reservoir of HPVs.
  
  
  METHODS
  HPV 63-induced warts were employed in this study, because the virus induces tiny warty lesions of a punctuate appearance in the plantar skin and shows peculiar intracytoplasmic inclusion bodies as a diagnostic histopathological marker of infection: this seemed to provide a useful model for the present study. Serial sections were obtained from the entire body of each biopsy specimen and were investigated histologically, immunohistochemically and using DNA-DNA in situ hybridization (ISH) for the histological localization of HPV 63 infection.
  
  
  RESULTS
  On microscopy, HPV 63 histopathological changes were seen closely associated with eccrine ducts. Using ISH, HPV 63 DNA was detected not only in keratinocytes resident around acrosyringia but also in the uppermost portion of the eccrine dermal duct. A few keratinocytes harbouring HPV 63 DNA were also identified in acrosyringeal areas in the normal plantar skin adjacent to the wart lesions.
  
  
  CONCLUSIONS
  On the basis of our results, it seems likely that HPV 63 targets keratinocytes resident in or around the eccrine ducts in the plantar skin. The results may also suggest that not only hair follicles but also eccrine ducts serve as reservoirs for certain HPV types, including HPV 63, especially in the nonhairy plantar skin.
authors.affiliation: Department of Dermatology, Kumamoto University School of Medicine, 1-1-1 Honjo, 860-0811 Kumamoto, Japan. kuriobo@kaiju.medic.kumamoto-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Egawa
mesh_terms: D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D004439:Eccrine Glands / Q000473:pathology / Q000821:virology*; D004817:Epidermis / Q000473:pathology / Q000821:virology*; D005260:Female; D005533:Foot Dermatoses / Q000473:pathology / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000821:virology; D008297:Male; D027383:Papillomaviridae / Q000302:isolation & purification*; D014860:Warts / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral