Single DNA molecules as probes of chromatographic surfaces.
YOYO-I-labeled lambda-DNA was employed as a nanoprobe for different functionalized surfaces to elucidate adsorption in chromatography. While the negatively charged backbone is not adsorbed, the 12-base unpaired ends of this DNA provide exposed purine and pyrimidine groups for adsorption. Self-assembled monolayers (SAMs) formed on gold substrate provide a wide range of choices of surface with well-defined and well-organized functional groups. Patterns of amino-terminated, carboxylic acid-terminated, and hydroxyl-terminated SAMs are generated by lithography. Patterns of metal oxides are generated spontaneously after deposition of metals. By recording the real-time dynamic motion of DNA molecules at the SAMs/aqueous interface, one can study the various parameters governing the retentivity of an analyte during chromatographic separation. Even subtle differences among adsorptive forces can be revealed.
Li
Hung-Wing
HW
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
Park
Hye-Young
HY
Porter
Marc D
MD
Yeung
Edward S
ES
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry; D013499:Surface Properties
D000588:Amines; D002264:Carboxylic Acids; D015342:DNA Probes; D006878:Hydroxides; D008670:Metals; D010087:Oxides; D006046:Gold