Crystallization and preliminary X-ray diffraction analysis of importin-alpha complexed with NLS peptidomimetics.
Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). The study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-alpha was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 A resolution. Preliminary electron density calculations show that the ligands are present in the crystals.
Fontes
Marcos R M
MR
Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil. fontes@ibb.unesp.br
Teh
Trazel
T
Riell
Ryan D
RD
Park
Seung Bum
SB
Standaert
Robert F
RF
Kobe
Bostjan
B
D003460:Crystallization; D008958:Models, Molecular; D018716:Molecular Mimicry; D019913:Nuclear Localization Signals / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D014961:X-Ray Diffraction; D028901:alpha Karyopherins / Q000737:chemistry* / Q000378:metabolism*
D019913:Nuclear Localization Signals; D028901:alpha Karyopherins