[Overexpression of TGFbeta1 increases ECM expression and adhesion of endothelial cells on matrix].
This study was aimed to examine the effectiveness of a gene transfer of human TGFbeta1 gene into endothelial cells and to determine whether TGFbeta1 increases ECM expression of endothelial cells. With the help of DOTAP, endothelial cells were transfected with pMAMneoTGFbeta1. The positive cell clones were selected with G418. The stable transfection and expression of TGFbeta1 in the endothelial cells were determined by immunofluorescence analysis. The expression levels of collagen I and fibronectin in the transfected and untransfected endothelial cells were determined by Western blot. The adhesion force between endothelial cells and matrix was determined by a micropipette technical system. The results showed abundant TGFbeta1 stable expression in the endothelial cells. TGFbeta1 gene was noted to increase collagen I and fibronectin expression and increase the adhesion between endothelial cells and matrix. These findings indicated that TGFbeta1 can be used in vascular tissue engineering for the enhancement of endothelial cells adhesion.
Zhu
Chuhong
C
Department of Anatomy, Biomechanics section under the Key lab for Biomechanics & Tissue Engineering of Ministry of Education, The Third Military Medical University, Chongqing, 400038, China.
Ying
Dajun
D
Mi
Jianhong
J
Dong
Shiwu
S
Zhang
Wei
W
Wei
Yong
Y
D002448:Cell Adhesion; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism* / Q000502:physiology; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
D024042:Collagen Type I; D005353:Fibronectins; D016212:Transforming Growth Factor beta