title: L7/Pcp-2-specific expression of Cre recombinase using knock-in approach.
abstract: We report a knock-in mouse expressing Cre recombinase from the translational initiation site (ATG) of the endogenous L7/Pcp-2 gene. The resulting Cre expression matches the pattern of L7/Pcp-2 expression that is restricted to cerebellar Purkinje cells and retinal cells. Moreover, the Cre mouse showed no significant behavioral abnormality. Thus, our novel Cre mouse can be used for generation of Purkinje cells and retinal cell-specific gene expression and/or knockout in mouse using the Cre/loxP system.
authors.affiliation: Department of Animal Genomics, Functional Genomics Institute, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Saito
authors.forename: Hideki
authors.initials: H
authors.lastname: Tsumura
authors.forename: Seiichi
authors.initials: S
authors.lastname: Otake
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nishida
authors.forename: Takahisa
authors.initials: T
authors.lastname: Furukawa
authors.forename: Noboru
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D017930:Genes, Reporter; D020662:Guanine Nucleotide Exchange Factors; D007150:Immunohistochemistry; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009479:Neuropeptides / Q000235:genetics*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D009479:Neuropeptides; C104971:Pcp2 protein, mouse; C045073:Cre recombinase; D019426:Integrases