Simultaneous determination of naproxen, ketoprofen and phenol red in samples from rat intestinal permeability studies: HPLC method development and validation.
A simple reversed-phase high performance liquid chromatographic method with UV detection at 270 nm was developed for simultaneous quantitation of ketoprofen and naproxen sodium along with phenol red as a non-absorbable marker for in situ permeability studies. The mobile phase was a mixture of 20% methanol, 28% of acetonitrile, 52% water and 0.4 ml triethylamine (adjusted to pH 3.2 using orthophosphoric acid). Analysis was run at a flow of 1.5 ml/min with a 20 min run time. The calibration curves were linear for all three compounds (r>0.999) across the concentration range of 15.6-250 microg/ml with a limit of quantitation of 0.3, 0.25 and 0.2 ng/ml for naproxen, ketoprofen and phenol red, respectively. The coefficient of variation for intra-assay and inter-assay precision was less than or equal to 5.3% and the accuracy was between 95.36 and 101.6%. Using the SPIP technique and the suggested HPLC method for sample analysis, the mean values of 1.17e(-4) (+/-0.28) cm/s and 0.97e(-4) (+/-0.2) cm/s were obtained for naproxen and ketoprofen, respectively.
Zakeri-Milani
Parvin
P
Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Barzegar-Jalali
Mohammad
M
Tajerzadeh
Hosniyeh
H
Azarmi
Yadollah
Y
Valizadeh
Hadi
H
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004345:Drug Industry / Q000379:methods*; D005021:Ethylamines / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000032:analysis*; D007422:Intestines / Q000187:drug effects*; D007660:Ketoprofen / Q000032:analysis*; D009288:Naproxen / Q000032:analysis*; D010477:Perfusion; D010539:Permeability; D010637:Phenolsulfonphthalein / Q000032:analysis*; D010756:Phosphoric Acids / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results; D013997:Time Factors; D014466:Ultraviolet Rays
D000097:Acetonitriles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005021:Ethylamines; D007202:Indicators and Reagents; D010756:Phosphoric Acids; D009288:Naproxen; D007660:Ketoprofen; C030242:phosphoric acid; D010637:Phenolsulfonphthalein; C016162:triethylamine; C032159:acetonitrile