Cold temperature-induced modifications to the composition and structure of the lipopolysaccharide of Yersinia pestis.
Following a report of variations in the lipopolysaccharide (LPS) structure of Yersinia pestis at mammalian (37 degrees C) and flea (25 degrees C) temperatures, a number of changes to the LPS structure were observed when the bacterium was cultivated at a temperature of winter-hibernating rodents (6 degrees C). In addition to one of the known Y. pestis LPS types, LPS of a new type was isolated from Y. pestis KM218 grown at 6 degrees C. The core of the latter differs in: (i) replacement of terminal galactose with terminal d-glycero-d-manno-heptose; (ii) phosphorylation of terminal oct-2-ulosonic acid with phosphoethanolamine; (iii) a lower content of GlcNAc, and; (iv) the absence of glycine; lipid A differs in the lack of any 4-amino-4-deoxyarabinose and presumably partial (di)oxygenation of a fatty acid(s). The data obtained suggest that cold temperature switches on an alternative mechanism of control of the synthesis of Y. pestis LPS.
Knirel
Yuriy A
YA
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia. knirel@ioc.ac.ru
Lindner
Buko
B
Vinogradov
Evgeny
E
Shaikhutdinova
Rima Z
RZ
Senchenkova
Sof'ya N
SN
Kocharova
Nina A
NA
Holst
Otto
O
Pier
Gerald B
GB
Anisimov
Andrey P
AP
D002240:Carbohydrate Sequence; D003080:Cold Temperature; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D015010:Yersinia pestis / Q000254:growth & development / Q000378:metabolism*
D008070:Lipopolysaccharides; C504013:lipopolysaccharide-6A, Yersinia pestis; C504014:lipopolysaccharide-6B, Yersinia pestis