title: Evaluation of the in vitro effect of albendazole, metronidazole and nitazoxanide on viability and structure of Giardia lamblia cysts.
abstract: Effect of albendazole, metronidazole and nitazoxanide on the viability and structure of Giardia lamblia cysts isolates from infected children. The viability was evaluated by inducing excystation in a low-pH solution followed by an incubation in TYI-S culture medium. Nitazoxanide exhibited potent inhibitory effect (100%), metronidazole (79%) and albendazole (31%). The analysis among groups indicated a rs = 0.75 and p < 0.05. By TEM the cysts incubated with albendazole did not show morphological changes; with metronidazole, the formation of residual bodies in the nucleus border was observed. Incubated with nitazoxanide the damage to the cyst wall was evident, with the formation of areas with a granular content and the presence of cytoplasmic components in the peritrophic space. Our results propose that nitazoxanide showed a high effect on the viability and structure of G. lamblia cysts.
authors.affiliation: Laboratory of Parasitology and Mycology, Children's Hospital of Mexico Federico Gómez, Mexico DF, Mexico. bernalhim@yahoo.com.mx
authors.forename: R
authors.initials: R
authors.lastname: Bernal-Redondo
authors.forename: L G
authors.initials: LG
authors.lastname: Martínez-Méndez
authors.forename: A
authors.initials: A
authors.lastname: Mendoza-Chavez
authors.forename: D
authors.initials: D
authors.lastname: Velasco-Perales
authors.forename: B
authors.initials: B
authors.lastname: Chavez-Munguia
mesh_terms: D015766:Albendazole / Q000494:pharmacology*; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D016829:Giardia lamblia / Q000187:drug effects* / Q000302:isolation & purification / Q000648:ultrastructure; D008795:Metronidazole / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D009574:Nitro Compounds; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: D000981:Antiprotozoal Agents; D009574:Nitro Compounds; D013844:Thiazoles; D008795:Metronidazole; D015766:Albendazole; C041747:nitazoxanide