A proton-dependent zinc uptake in PC12 cells.
Intracellular pH in pheochromocytoma (PC12) cells was manipulated by 'acid loading' the cells and the effect of such a change on radioactive zinc uptake was studied. It was found that zinc uptake was stimulated in cells loaded with protons without causing any measurable change in the intracellular pH. To confirm our assumption that the proton flux due to zinc entry is too small to be measured, we calculated the pH change that one would expect because of zinc influx. The intrinsic buffer capacity of PC12 cells was determined to be 8.03 mM/pH unit and was used in these calculations. It was found that at the five-minute incubation, zinc uptake occurring under our experimental conditions could cause a pH change of 0.000277 pH units per minute (assuming a 1:2 zinc:proton stoichiometry). This study adds a new dimension towards understanding the role played by intracellular pH in causing zinc entry into cells.
Balaji
Rengarajan V
RV
Program in Neuroscience, Department of Biological Sciences, Ohio University, Athens, OH-45701, USA. rvbalaji@utmb.edu
Colvin
Robert A
RA
D000818:Animals; D001578:Benzopyrans; D002021:Buffers; D002138:Calibration; D002462:Cell Membrane / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009284:Naphthols; D016716:PC12 Cells; D011522:Protons; D051381:Rats; D012235:Rhodamines; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000378:metabolism*; D015037:Zinc Radioisotopes
D001578:Benzopyrans; D002021:Buffers; D009284:Naphthols; D011522:Protons; D012235:Rhodamines; D017923:Sodium-Hydrogen Exchangers; D015037:Zinc Radioisotopes; C091666:seminaphthorhodaminefluoride; D015032:Zinc