Effect of 3, 4-dihydroxyacetophenone on Na+, K+ -ATPase activity of injured mitochondria and the oxygen consumption of brain cells of rat.
OBJECTIVE
To investigate the effect of 3,4-dihydroxyacetophenone (alpha-DHAP) on Na+, K+ -ATPase activity of injured brain mitochondria induced by ascorbate-FeSO4 and the oxygen consumption of rat brain cells stimulated by ADP.


METHODS
Na+, K+ -ATPase activity was determined according to the method of inorganic phosphate. Swelling of the brain mitochondria was detected with the method of spectrophotometer. Lipid peroxidation was detected according to the thiobarbituric acid method of spectrophotometer. Oxygen consumption was measured by oxygen electrode method.


RESULTS
The decrease of Na+, K+ -ATPase activity, mitochondria swelling and formation of lipid peroxidation were shown in rat brain mitochondria and cells induced by ascorbate-FeSO4. alpha-DHAP was shown to increase the activity of Na+, K+ -ATPase, decrease the mitochondria swelling and inhibit the production of lipid peroxidation of brain mitochondria and cells induced by ascorbate and FeSO4. alpha-DHAP can also reduce the oxygen consumption of brain cells stimulated by ADP.


CONCLUSIONS
alpha-DHAP can protect the structure and the function of brain mitochondria and cells by scavenging the free radical and resisting the reaction of lipid peroxidation.
Lu
Xue-ya
XY
Department of Biochemistry, Beijing University of Traditional Chinese Medicine, Beijing 100029, China. Luxueya-bp@163.com
Chen
Wei-chang
WC
D000098:Acetophenones / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000098:Acetophenones; D016166:Free Radical Scavengers; C032940:3,4-dihydroxyacetophenone; D000244:Adenosine Diphosphate; D000254:Sodium-Potassium-Exchanging ATPase