title: The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.
abstract: The Cdc14 dual-specificity phosphatases regulate key events in the eukaryotic cell cycle. However, little is known about the function of mammalian CDC14B family members. Here, we demonstrate that subcellular localization of CDC14B protein is cell cycle regulated. CDC14B can bind, bundle, and stabilize microtubules in vitro independently of its catalytic activity. Basic amino acid residues within the nucleolar targeting domain are important for both retaining CDC14B in the nucleolus and preventing microtubule bundling. Overexpression of CDC14B resulted in the formation of cytoplasmic CDC14B and microtubule bundles in interphase cells. These microtubule bundles were resistant to microtubule depolymerization reagents and enriched in acetylated alpha-tubulin. Expression of cytoplasmic forms of CDC14B impaired microtubule nucleation from the microtubule organization center. CDC14B is thus a novel microtubule-bundling and -stabilizing protein, whose regulated subcellular localization may help modulate spindle and microtubule dynamics in mitosis.
authors.affiliation: Life Sciences Division, Oak Ridge National Laboratory, Bethel Valley Rd., Oak Ridge, TN 37831, USA.
authors.forename: Hyekyung P
authors.initials: HP
authors.lastname: Cho
authors.forename: Yie
authors.initials: Y
authors.lastname: Liu
authors.forename: Marla
authors.initials: M
authors.lastname: Gomez
authors.forename: John
authors.initials: J
authors.lastname: Dunlap
authors.forename: Mike
authors.initials: M
authors.lastname: Tyers
authors.forename: Yisong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics; D003593:Cytoplasm / Q000737:chemistry; D054637:Dual-Specificity Phosphatases; D005816:Genetic Complementation Test; D006801:Humans; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C074860:CDC14 protein, S cerevisiae; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D014404:Tubulin; C474200:CDC14B protein, human; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases