[Expression of heat shock protein 70 in intestinal mucosa during the early stage after severe burns and its significance].
OBJECTIVE
To study the expression of heat shock protein 70 (HSP70) in intestinal mucosa during the early stage after severe burn injury and its significance.


METHODS
With a model of 30% total body surface area (TBSA) full-thickness burned rats, the expression and distribution of HSP70 and heat shock factor-1 (HSF1) in intestinal mucosal were determined by reverse transcription-polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry at 3, 6, 12, 24 and 48 hours postburn.


RESULTS
The expression of HSP70 mRNA and protein in intestinal mucosa increased markedly at 3 hours after severe burns, peaked at 6 and 12 hours, and lasted for 48 hours postburn (all P<0.01). Following a slight decline at 3 hours postburn, the expression of HSF1 increased gradually, and reached a highest level at 48 hours postburn (all P<0.01).


CONCLUSIONS
The expression of HSP70 and HSF1 markedly increase in intestinal mucosa following severe burn injury. It is suggested that the self-protective mechanism of cells might explain the increase of HSP70 expression.
Yuan
Zhi-qiang
ZQ
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Peng
Yi-zhi
YZ
Li
Xiao-lu
XL
Huang
Yue-sheng
YS
Yang
Zong-cheng
ZC
D000818:Animals; D002056:Burns / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; C000619709:Hsf1 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors