Natrinema altunense sp. nov., an extremely halophilic archaeon isolated from a salt lake in Altun Mountain in Xinjiang, China.
A novel extremely halophilic strain, AJ2(T), was isolated from Ayakekum salt lake located in the Altun Mountain National Nature Reserve in Xinjiang, China. This isolate was neutrophilic, motile and grew in a wide range of MgCl(2) concentrations (0.005-1.0 M). The major polar lipids of the isolate were C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. A comprehensive 16S rRNA gene sequence analysis revealed that the isolate shared 96.6-97.7 % sequence identity with Natrinema species. The isolate, however, could be genetically differentiated from these species by DNA-DNA hybridization analysis and on the basis of its physiological properties. On the basis of the polyphasic evidence, strain AJ2(T) (= AS 1.3731(T) = JCM 12890(T)) represents the type strain of a novel species, for which the name Natrinema altunense sp. nov. is proposed.
Xu
Xue-Wei
XW
Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, P. R. China.|College of Life Sciences, Zhejiang University, 38 Zheda Road, Hangzhou 310027, P. R. China.
Ren
Pei-Gen
PG
Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, P. R. China.
Liu
Shuang-Jiang
SJ
Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, P. R. China.
Wu
Min
M
College of Life Sciences, Zhejiang University, 38 Zheda Road, Hangzhou 310027, P. R. China.
Zhou
Pei-Jin
PJ
Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, P. R. China.
D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D019766:Genes, Archaeal; D020459:Genes, rRNA; D006131:Growth Inhibitors / Q000494:pharmacology; D006216:Halobacteriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006131:Growth Inhibitors; D010743:Phospholipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride