title: Radiomodulatory effect of liposome encapsulated AK-2123 on tumor in mice exposed to hepatocarcinogen.
abstract: An attempt was made to evaluate the whole body gamma-radiation effect on tumor in the presence of free and liposome encapsulated AK-2123, a hypoxic cell radiosensitizer that has widely been used in combination with a number of cancer therapies such as thermotherapy, chemotherapy and radiotherapy. Entrapment efficiency of AK-2123 into liposome was determined by LASER Raman spectroscopy. Cancer induction in mice was carried out by repeated exposure of N-nitrosodiethylamine (DEN) in combination with partial hepatectomy. Parameters such as marker enzymes activities (GGT and AChE), rates of nucleic acid synthesis, viability modification factor and the histology of liver tissues monitored, supported the induction of cancer in liver. In addition, the effect of free as well as liposome encapsulated AK-2123 on haemopoietic parameters were also studied. It was observed that AK-2123 after incorporation into liposome afforded more efficient radiomodulatory effects than that of free AK-2123 as determined by the above-mentioned parameters. Neither free AK-2123 nor liposome encapsulated AK-2123 showed any detectable toxic effects on the mice. Thus, it is seen that treatment of cancer with a combination of radiation, a radiomodifier and a drug delivery system, opens a wide scope for exploitation for the improvement of existing cancer therapies.
authors.affiliation: Immunology Laboratory, Department of Biochemistry, North Eastern Hill University, Shillong-793022, India. a_alam51@yahoo.com
authors.forename: Anis
authors.initials: A
authors.lastname: Alam
authors.forename: Chowphi Chen
authors.initials: CC
authors.lastname: Rapthap
authors.forename: Laishram Indira
authors.initials: LI
authors.lastname: Singha
authors.forename: R N
authors.initials: RN
authors.lastname: Sharan
authors.forename: Vinod
authors.initials: V
authors.lastname: Singh
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D003131:Combined Modality Therapy; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D004052:Diethylnitrosamine / Q000633:toxicity; D005720:Gamma Rays; D008081:Liposomes / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D014916:Whole-Body Irradiation; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
chemical_list: D002273:Carcinogens; D008081:Liposomes; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D004052:Diethylnitrosamine; D012313:RNA; D004247:DNA; D005723:gamma-Glutamyltransferase; D000110:Acetylcholinesterase