title: Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria.
abstract: Strain Kw07T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the alpha-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033T(97.8%). Chemotaxonomic data revealed that strain Kw07T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C18:1 omega7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07T represents a distinct species. Based on these data, Kw07T (=KCTC 12209T=NBRC 100800T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.
authors.affiliation: Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
authors.forename: Myungjin
authors.initials: M
authors.lastname: Lee
authors.forename: Sung-Taik
authors.initials: ST
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D042301:Sphingomonadaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D001617:beta-Glucosidase