title: Analysis of ameloblastomas by comparative genomic hybridization and fluorescence in situ hybridization.
abstract: In order to characterize the chromosomal alterations in ameloblastomas, a combination of comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) techniques was performed on 9 tumors. Chromosomal alterations including a gain at 1q and losses at 1pter, 10q, and 22q could be detected by CGH only in 1 tumor. Interphase FISH analysis, using centromeric probes for chromosomes 1, 10, and 22 as well as region-specific probes for 1p36 and 10q26, revealed the most frequent alterations to exist in the tumor with the abnormal CGH profile. These alterations included marked to slight increases of monosomic cells for chromosome 10 (91.5%), 10q26 (35.8%), 1p36 (24.4%), and chromosome 22 (18.8%), as well as significant elevations of trisomic cells for chromosome 1 (41.2%). Moreover, FISH analysis revealed a frequent loss of chromosome 22 in all tumors examined, except for one lesion, indicating that loss of the entire or a part of this chromosome is a common event in ameloblastomas, possibly being a predisposing factor to ameloblastoma tumorigenesis.
authors.affiliation: Department of Oral and Maxillofacial Sciences, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. toida@cc.gifu-u.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Toida
authors.forename: Margit
authors.initials: M
authors.lastname: Balázs
authors.forename: Andrea
authors.initials: A
authors.lastname: Treszl
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Rákosy
authors.forename: Keizo
authors.initials: K
authors.lastname: Kato
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Matsui
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Suwa
authors.forename: Daijiro
authors.initials: D
authors.lastname: Hatakeyama
authors.forename: Hiroki
authors.initials: H
authors.lastname: Makita
authors.forename: Sojiro
authors.initials: S
authors.lastname: Mori
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yamashita
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Róza
authors.initials: R
authors.lastname: Adány
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002879:Chromosomes, Human, Pair 10; D002892:Chromosomes, Human, Pair 22; D005260:Female; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization