CD85j (leukocyte Ig-like receptor-1/Ig-like transcript 2) inhibits human osteoclast-associated receptor-mediated activation of human dendritic cells.
Immature dendritic cells (DCs) derived from freshly isolated human monocytes were used to evaluate the effect of the inhibiting receptor CD85j (leukocyte Ig-like receptor-1/ILT2) on activation induced by cross-linking of the human osteoclast-associated receptor (hOSCAR). CD85j and hOSCAR were expressed consistently at the same density on monocytes and on monocyte-derived DCs (both immature and mature). Cross-linking of hOSCAR, which activates via the FcR-associated gamma-chain, induced Ca(2+) flux in DCs. Concomitant cross-linking of anti-CD85j mAb abolished this early activation event. Likewise, CD85j stimulation strongly reduced IL-8 and IL-12 production by hOSCAR-activated DCs. Inhibition of DCs via CD85j also impaired their ability to enhance Ag-specific T cell proliferation induced by hOSCAR. Finally, because hOSCAR prevents apoptosis of DCs in the absence of growth/survival factors, CD85j cross-linking was able to counteract completely this antiapoptotic effect and to reduce Bcl-2 expression enhanced by hOSCAR stimulation. Thus, CD85j is an inhibiting receptor that is functional in human DCs.
Tenca
Claudya
C
Department of Experimental Medicine, Human Anatomy Section, University of Genoa, Italy.
Merlo
Andrea
A
Merck
Estelle
E
Bates
Elizabeth E M
EE
Saverino
Daniele
D
Simone
Rita
R
Zarcone
Daniela
D
Trinchieri
Giorgio
G
Grossi
Carlo E
CE
Ciccone
Ermanno
E
D015703:Antigens, CD / Q000096:biosynthesis / Q000502:physiology*; D017209:Apoptosis / Q000276:immunology; D002118:Calcium / Q000037:antagonists & inhibitors / Q000378:metabolism; D020013:Calcium Signaling / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015536:Down-Regulation / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006131:Growth Inhibitors / Q000502:physiology*; D006801:Humans; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010010:Osteoclasts / Q000276:immunology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000502:physiology*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
D015703:Antigens, CD; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D006131:Growth Inhibitors; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; C512828:OSCAR protein, human; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D002118:Calcium