title: Comparative study on effects of burn-blast combined injury and burn-firearm combined injury complicated with seawater immersion on vascular endothelial cells.
abstract: OBJECTIVE
  To comparatively study the effects and mechanisms of burn-blast combined injury and burn-firearm combined injury complicated with seawater immersion on vascular endothelial cells.
  
  
  METHODS
  A total of 40 healthy adult hybrid dogs of both sexes, weighing 12-15 kg, were used in this study. Randomly-selected 20 dogs were established as models of burn-blast combined injury (the burn-blast injury group) and the other 20 dogs as models of burn-firearm combined injury (the burn-firearm injury group). Then the wounds of all the dogs were immediately immersed in seawater for 4 hours, and then they were taken out from the seawater. Blood samples were withdrawn from the central vein of the dogs before injury, and at 4, 7, 10, 20, and 28 hours after injury to measure the circulating endothelial cells and the von Willebrand factor.
  
  
  RESULTS
  Circulating endothelial cells increased significantly at 4 hours after injury in all the dogs. But they reached peak at 7 hours after injury in the burn-blast injury group and at 28 hours after injury in the burn-firearm injury group. The changes of circulating endothelial cells in the burn-blast injury group were significantly different from those in the burn-firearm injury group at 4, 7, 20, and 28 hours after injury (P < 0.01). The von Willebrand factor reached peak at 4 hours after injury in the burn-blast injury group and at 28 hours in the burn-firearm injury group. The changes of von Willebrand factor in the burn-blast injury group were significantly different from those in the burn-firearm injury group at 4, 20, and 28 hours after injury (P < 0.01).
  
  
  CONCLUSIONS
  In burn-blast injury combined with seawater immersion, the vascular endothelial cells changed most significantly at 4 hours or 7 hours after injury, while burn-firearm injury combined with seawater immersion have the same at 20 hours or 28 hours after injury.
authors.affiliation: Department of Anesthesia, Daping Hospital and Research Institute of Field Surgery, Third Military Medical University, Chongqing 400042, China. cqyanhong@163.com
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Xi-nan
authors.initials: XN
authors.lastname: Lai
authors.forename: Heng-jiang
authors.initials: HJ
authors.lastname: Ge
mesh_terms: D000818:Animals; D001753:Blast Injuries / Q000473:pathology* / Q000503:physiopathology; D002056:Burns / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D007101:Immersion; D015601:Injury Severity Score; D008297:Male; D009102:Multiple Organ Failure / Q000503:physiopathology; D009104:Multiple Trauma / Q000473:pathology / Q000503:physiopathology; D011336:Probability; D011897:Random Allocation; D012623:Seawater; D012680:Sensitivity and Specificity; D014945:Wound Healing / Q000502:physiology*; D014948:Wounds, Gunshot / Q000473:pathology* / Q000503:physiopathology