Cardiac volume overload rapidly induces oxidative stress-mediated myocyte apoptosis and hypertrophy.
Oxidative stress stimulates both growth and apoptosis in cardiac myocytes in vitro. We investigated the role of oxidative stress in the initial phases of cardiac remodeling induced in an animal model by volume overload. As plausible candidates for a connection between oxidative stress and cardiomyocyte apoptosis or hypertrophy, we explored the behaviour of two MAPKs, specifically JNK and ERK. At 48 h of overload, the greatest increase in oxidative stress coincided with a peak of cardiomyocyte apoptosis. This was possibly induced through the mitochondrial metabolism, as evidenced by the release of cytochrome c and a significant increase in the active forms of caspase-9 and -3, but not caspase-8. Oxidative stress markers significantly decreased at 96 h of overload, combined with a marked attenuation of apoptosis and the appearance of hypertrophy. The highest levels of JNK and the lowest levels of ERK phosphorylation were observed at 48 h of overload. Conversely, a sharp increase in ERK phosphorylation was detected at 96 h of overload coinciding with the hypertrophic response. Together these results show that oxidative stress is an early and transient event in myocardial volume overload. They suggest that oxidative stress mediates amplitude dependent apoptotic and hypertrophic responses in cardiomyocytes through the selective activation of, respectively, JNK and ERK.
Fiorillo
C
C
Department of Biochemical Sciences, Viale Morgagni 50, 50134 Florence, Italy. claudia.fiorillo@unifi.it
Nediani
C
C
Ponziani
V
V
Giannini
L
L
Celli
A
A
Nassi
N
N
Formigli
L
L
Perna
A M
AM
Nassi
P
P
D000818:Animals; D017209:Apoptosis; D002306:Cardiac Volume / Q000502:physiology*; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D048429:Cell Size; D045304:Cytochromes c / Q000378:metabolism; D004452:Echocardiography; D004789:Enzyme Activation; D006439:Hemodynamics; D006984:Hypertrophy; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D010766:Phosphorylation; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D034421:Sus scrofa; D013997:Time Factors
D008315:Malondialdehyde; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases