Intraepithelial lymphocytes express junctional molecules in murine small intestine.
Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), beta-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer's patches did not. Gammadelta IEL showed higher level of these expressions than alphabeta IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC.
Inagaki-Ohara
Kyoko
K
Parasitic Diseases Unit, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan. INAGAKI@med.miyazaki-u.ac.jp
Sawaguchi
Akira
A
Suganuma
Tatsuo
T
Matsuzaki
Goro
G
Nawa
Yukifumi
Y
D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D017931:DNA Primers; D006706:Homeostasis; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D062726:Junctional Adhesion Molecules; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D062726:Junctional Adhesion Molecules