Expression of the vanilloid receptor TRPV1 in rat dorsal root ganglion neurons supports different roles of the receptor in visceral and cutaneous afferents.
A combination of tracing and multiple color immunofluorescence revealed that 69% of rat dorsal root ganglion (DRG) neurons innervating the urinary bladder expressed the vanilloid receptor TRPV1. In contrast, only 32% of DRG neurons innervating the skin of the L6 dermatome expressed TRPV1. However, a similar fraction of visceral (60-62%) and of cutaneous (59-60%) TRPV1-positive DRG neurons expressed the peptidergic markers substance P and calcitonin gene-related peptide, while the fraction of TRPV1-positive neurons that was labeled by the non-peptidergic marker Isolectin B4 was 58% for cutaneous and only 24% for visceral afferents. These results underscore differences of expression of functional markers in visceral and cutaneous afferents and support different mechanisms of activation of TRPV1 in viscera and in skin.
Hwang
Se Jin
SJ
Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Seoul 133-791, South Korea.
Oh
Jung Min
JM
Valtschanoff
Juli G
JG
D000344:Afferent Pathways / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D007473:Ion Channels / Q000096:biosynthesis*; D037102:Lectins / Q000096:biosynthesis; D008297:Male; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation*; D013373:Substance P / Q000096:biosynthesis; D050916:TRPV Cation Channels; D001743:Urinary Bladder / Q000294:innervation; D053675:Versicans; D017833:Visceral Afferents / Q000378:metabolism*
D006023:Glycoproteins; D007473:Ion Channels; D037102:Lectins; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C508324:Vcan protein, rat; D053675:Versicans; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide