Rapid screening procedures for identification of succinic acid producers.
Succinic acid, an intermediate of tricarboxylic acid cycle, is produced and accumulated by anaerobic microorganisms. The long-standing interest in the production of this organic acid is because it is a key compound in producing more than 30 commercially important products. The detection of succinic acid is generally carried out by gas chromatography (GC), enzymatic assays, ion-exclusion chromatography (IEC) or by high performance liquid chromatography (HPLC). However, these methods are time consuming, require sophisticated instrumentation and are expensive. In the present investigation we are reporting two rapid, cost effective screening methods for the detection of this important organic acid. These methods can be utilized to screen a large number of microbes producing succinic acid in a very short span of time.
Agarwal
Lata
L
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India.
Isar
Jasmine
J
Saxena
Rajendra K
RK
D001419:Bacteria / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes; D005658:Fungi / Q000378:metabolism*; D015203:Reproducibility of Results; D012118:Resorcinols; D019802:Succinic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013464:Sulfuric Acids
D005456:Fluorescent Dyes; D012118:Resorcinols; D013464:Sulfuric Acids; D019802:Succinic Acid; C033158:sulfuric acid; C031389:resorcinol