Identification and sequencing of Salmonella enterica serotype typhi isolates obtained from patients with perforation and non-perforation typhoid fever.
We describe the characterization of Salmonella enterica serovar Typhi, isolated from the blood of patients with perforation and non-perforation typhoid fever, by a combination of conventional microbiological tests, 16S rRNA gene sequencing, and flagellin gene and CDP-tyvelose epimerase (rfbE) gene sequencing. The 16S rRNA gene sequencing showed that there were four base mutations from perforation samples and only three from non-perforation samples. These findings indicated that the isolates were a strain of Salmonella enterica. The flagellin gene sequences from the two groups were 100% identical to that of the H1-d flagellin gene of serovar Typhi. Sequences of the rfbE from both groups were also 100% identical.
Massi
Muhammad Nasrum
MN
International Center for Medical Research, Kobe University School of Medicine, Japan.
Shirakawa
Toshiro
T
Gotoh
Akinobu
A
Hatta
Mochammad
M
Kawabata
Masato
M
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012480:Salmonella Infections / Q000097:blood / Q000175:diagnosis*; D012485:Salmonella typhi / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D012720:Severity of Illness Index; D014435:Typhoid Fever / Q000175:diagnosis / Q000382:microbiology*