Changes in sucrose and glycerol content in garlic shoot tips during freezing using PVS3 solution.
Changes in moisture content (MC), sucrose and glycerol concentration in garlic shoot tips were monitored during loading and unloading with PVS3 solution. Upon PVS3 treatment, shoot tip MC decreased rapidly and sucrose and glycerol concentrations increased rapidly during the first 30 min. Sucrose and glycerol concentrations increased more slowly thereafter. Shoot tip MC in after PVS3 treatment was affected by their size, but not by sucrose concentration of the preculture medium. As the size of shoot tips increased, so their MC increased after PVS3 treatment. However, sucrose and glycerol concentrations decreased after PVS3 incubation, and concentrations in dehydrated shoot tips were much lower than those measured in non-air dried controls. During unloading with 1.2 M sucrose medium, shoot tip MC increased rapidly during the first 10 min, whereas glycerol concentration decreased steadily over 90 min. Loading and unloading of PVS3 solution in garlic shoot tips follows the principle of solute bulk flow.
Kim
Jung-Bong
JB
National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Korea.
Kim
Haeng-Hoon
HH
Baek
Hyung-Jin
HJ
Cho
Eun-Gi
EG
Kim
Yong-Hwan
YH
Engelmann
Florent
F
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000493:pharmacokinetics; D003890:Desiccation; D005737:Garlic / Q000378:metabolism* / Q000648:ultrastructure; D005990:Glycerol / Q000493:pharmacokinetics*; D046529:Microscopy, Electron, Transmission; D018520:Plant Shoots / Q000378:metabolism* / Q000648:ultrastructure; D013395:Sucrose / Q000493:pharmacokinetics*; D014867:Water / Q000378:metabolism
D003451:Cryoprotective Agents; D014867:Water; D013395:Sucrose; D005990:Glycerol