title: Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine.
abstract: The major components of the plant curcuma longa are the curcuminoids that include curcumin, demethoxycurcumin and bisdemethoxycurcumin. It has been reported the curcuminoids have some important activities. A new CZE method with diode array detection has been developed for the separation and determination of the curcumin, demethoxycurcumin and bisdemethoxycurcumin. Three curcuminoids could be readily separated within 7 min with a 15 mM sodium tetraborate buffer containing 10% methanol (v/v) at pH 10.8, 25 kV and 30 degrees C. The method has been validated and shows good performance with respect to selectivity, reproducibility, linearity, limits of detection and recovery. The proposed method was successfully applied to determine the curcuminoids in urine.
authors.affiliation: National Chromatographic R.&A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, PR China.
authors.forename: Kailong
authors.initials: K
authors.lastname: Yuan
authors.forename: Qianfeng
authors.initials: Q
authors.lastname: Weng
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Xiong
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D002021:Buffers; D002138:Calibration; D003474:Curcumin / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D003474:Curcumin