Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
The aim of this study was to evaluate the contribution of chemokine receptor CXCR3 and the corresponding ligands CXCL10 and CXCL11 to the recruitment of peripheral blood (PB) memory CD4+ T-cells into the cerebrospinal fluid (CSF) of patients with acute neuroborreliosis. Percentages of memory CD45RO+CD4+ T-cells expressing CXCR3 and CCR5 were significantly enriched in the CSF compared to the PB. Concentrations of CXCL10 and CXCL11 in the CSF of neuroborreliosis patients were significantly higher compared with the corresponding serum samples. Our results suggest that CXCL10 and CXCL11 create a chemokine gradient between the CSF and serum and recruite CXCR3-expressing memory CD4+ T-cells into the CSF of neuroborreliosis patients and that CCR5 also plays a role in this process.
Lepej
Snjezana Zidovec
SZ
University Hospital for Infectious Diseases, Dr. Fran Mihaljević, Mirogojska 8, 10000 Zagreb, Croatia. Snjezana.Zidovec.Lepej@bfm.hr
Rode
Oktavija Daković
OD
Jeren
Tatjana
T
Vince
Adriana
A
Remenar
Anica
A
Barsić
Bruno
B
D000328:Adult; D001898:Borrelia / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology*; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D019743:Chemokines, CXC / Q000097:blood / Q000502:physiology*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D020852:Lyme Neuroborreliosis / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D019713:Receptors, CCR5 / Q000096:biosynthesis* / Q000097:blood / Q000502:physiology; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000097:blood / Q000502:physiology; D015854:Up-Regulation / Q000276:immunology*
C515202:CXCL11 protein, human; C515187:CXCR3 protein, human; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D019743:Chemokines, CXC; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine