title: [Universal method for single nucleotide substitution identification].
abstract: A new approach to the identification of point mutations by allele-specific PCR was proposed. The mutation R408W of the human phenylalanine hydroxylase gene was used as a model. A high specificity of the approach was achieved by the use of primers partially complementary to the genomic DNA. Polyethylene glycol covalently attached to one of the allele-specific primers provides for the differential identification of the PCR products due to a change in electrophoretic mobility.
authors.forename: E V
authors.initials: EV
authors.lastname: Brenner
authors.forename: E M
authors.initials: EM
authors.lastname: Ivanova
authors.forename: D V
authors.initials: DV
authors.lastname: Pyshnyĭ
authors.forename: I V
authors.initials: IV
authors.lastname: Morozov
mesh_terms: D000483:Alleles; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010651:Phenylalanine Hydroxylase / Q000235:genetics; D010661:Phenylketonurias / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D010651:Phenylalanine Hydroxylase