CtBP3/BARS drives membrane fission in dynamin-independent transport pathways.
Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.
Bonazzi
Matteo
M
Laboratory of Membrane Traffic, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (Chieti), Italy.
Spanò
Stefania
S
Turacchio
Gabriele
G
Cericola
Claudia
C
Valente
Carmen
C
Colanzi
Antonino
A
Kweon
Hee Seok
HS
Hsu
Victor W
VW
Polishchuck
Elena V
EV
Polishchuck
Roman S
RS
Sallese
Michele
M
Pulvirenti
Teodoro
T
Corda
Daniela
D
Luini
Alberto
A
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D004285:Dogs; D034281:Dynamins / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D015388:Organelles / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism* / Q000648:ultrastructure
D002352:Carrier Proteins; C119945:Ctbp1 protein, rat; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D034281:Dynamins