Fas gene promoter -670 polymorphism (A/G) is associated with cervical carcinogenesis.
OBJECTIVE
To investigate the biological significance of single nucleotide polymorphism (SNP) at Fas gene promoter in cervical carcinogenesis.


METHODS
SNP at -670 of Fas gene promoter (A/G) together with human papillomavirus (HPV) types were examined in a total of 279 cervical smear samples and 8 human cervical squamous carcinoma cell lines using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques.


RESULTS
49 patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV and GA + GG genotype than 167 with low-grade SIL (LSIL) and 63 controls. G allele frequency was also higher in HSIL than in LSIL and controls. There was an increased OR (6.00; CI, 1.32-27.37; P = 0.021) for GA + GG genotype in HSIL cases compared to controls among 96 patients with high-risk HPV. 7 of 8 cervical carcinoma cell lines also showed GA or GG genotype.


CONCLUSIONS
Fas gene promoter -670 polymorphism (A/G) may be closely associated with cervical carcinogenesis in a Japanese population.
Ueda
Masatsugu
M
Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan. gyn017@poh.osaka-med.ac.jp
Hung
Yao-Ching
YC
Terai
Yoshito
Y
Yamaguchi
Hiroyuki
H
Saito
Junko
J
Nunobiki
Osamu
O
Noda
Sadamu
S
Ueki
Minoru
M
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D045744:Cell Line, Tumor; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000145:classification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D019014:fas Receptor / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology
D019014:fas Receptor