Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines.
Scaffold or matrix attachment region (S/MAR) genetic elements have previously been proposed to insulate transgenes from repressive effects linked to their site of integration within the host cell genome. We have evaluated their use in various stable transfection settings to increase the production of recombinant proteins such as monoclonal antibodies from Chinese hamster ovary (CHO) cell lines. Using the green fluorescent protein coding sequence, we show that S/MAR elements mediate a dual effect on the population of transfected cells. First, S/MAR elements almost fully abolish the occurrence of cell clones that express little transgene that may result from transgene integration in an unfavorable chromosomal environment. Second, they increase the overall expression of the transgene over the whole range of expression levels, allowing the detection of cells with significantly higher levels of transgene expression. An optimal setting was identified as the addition of a S/MAR element both in cis (on the transgene expression vector) and in trans (co-transfected on a separate plasmid). When used to express immunoglobulins, the S/MAR element enabled cell clones with high and stable levels of expression to be isolated following the analysis of a few cell lines generated without transgene amplification procedures.
Girod
Pierre-Alain
PA
Laboratory of Molecular Biotechnology, Center for Biotechnology UNIL-EPFL, University of Lausanne, Switzerland.
Zahn-Zabal
Monique
M
Mermod
Nicolas
N
D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D002645:Chickens; D006224:Cricetinae; D003412:Cricetulus; D024861:Genetic Enhancement / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045170:Matrix Attachment Regions / Q000235:genetics*; D009113:Muramidase / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*
D007074:Immunoglobulin G; D036961:Matrix Attachment Region Binding Proteins; D011994:Recombinant Proteins; D009113:Muramidase