Association between DNA variant sites in the apolipoprotein A5 gene and coronary heart disease in Chinese.
The recently discovered apolipoprotein A5 ( APOA5 ) gene has been shown to be important in determining plasma triglyceride levels, a major cardiovascular disease risk factor. We searched for possible associations of the APOA5 gene polymorphisms S19W and -1131T>C with coronary heart disease (CHD) in a Chinese population. A total of 483 Chinese CHD patients and 502 control non-CHD subjects were genotyped by polymerase chain reaction-restriction fragment length polymorphism for these 2 single nucleotide polymorphisms. We found that the minor allele 19W was observed only in CHD patients and not in controls, with allelic frequencies of 0.047 and 0.000, respectively ( P < .000001), and the minor allele -1131C was significantly higher in CHD patients than in controls (0.391 vs 0.299, P < .0001). These results suggest that both the S19W and -1131T>C variations in the APOA5 gene are associated with the CHD and appear to be 2 genetic risk factors for CHD susceptibility in Chinese. Moreover, we found that triglyceride levels were significantly higher in -1131C carriers than in -1131T subjects of the control group and that high-density-lipoprotein cholesterol was decreased in -1131C carriers among CHD patients.
Liu
Hekun
H
State Key Laboratory of Biotherapy, Division of Human Morbid Genomics, Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Sizhong
S
Lin
Jianyin
J
Li
Hai
H
Huang
Aimin
A
Xiao
Cuiying
C
Li
Xuefei
X
Su
Zhiguang
Z
Wang
Chunting
C
Nebert
Daniel W
DW
Zhou
Bing
B
Zheng
Keqin
K
Shi
Jiajun
J
Li
Guixin
G
Huang
Dejia
D
D000072040:Apolipoprotein A-V; D001053:Apolipoproteins / Q000235:genetics*; D001054:Apolipoproteins A; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D003596:Cytosine; D004247:DNA / Q000235:genetics*; D005215:Fasting / Q000097:blood; D005260:Female; D014644:Genetic Variation; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012694:Serine; D013941:Thymine; D014280:Triglycerides / Q000097:blood; D014364:Tryptophan
C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001053:Apolipoproteins; D001054:Apolipoproteins A; D008076:Cholesterol, HDL; D014280:Triglycerides; D012694:Serine; D014364:Tryptophan; D003596:Cytosine; D004247:DNA; D013941:Thymine