2,5-Hexanedione induced decrease in cytoskeletal proteins of rat sciatic-tibial nerve.
Exposure chronically to n-hexane produces peripheral-central axonopathy mediated by 2,5-hexanedione (HD). Previous studies have demonstrated decreases in neurofilament (NF) contents of peripheral and central nervous regions from rats intoxicated with HD, and recent analysis has demonstrated that axonal atrophy, instead of NF-filled swellings, is a specific component of morphologic alterations. To deeply investigate the alterations of cytoskeletal proteins in HD peripheral neuropathy, the relative levels of NF-L, NF-M, NF-H, alpha-tubulin, beta-tubulin and beta-actin of rat sciatic-tibial nerves were determined by SDS-PAGE and immunoblotting. HD was administrated to Wistar rats by intraperitoneal injection at dosage of 200 or 400 mg/kg/day (five-times per week). Rats were sacrificed after 6 weeks of treatment, and sciatic-tibial nerves were dissected, homogenized, and used for the determination of cytoskeletal proteins. Except for supernatant NF-L that could not be assayed, the results showed HD intoxication was associated with significant decreases in NF subunits in both of the supernatant and the pellet fractions of sciatic-tibial nerve homogenates (P < 0.01), and obvious reductions in alpha-tubulin, beta-tubulin and beta-actin only in the supernatant (P < 0.05 or P < 0.01). Among these alterations, the falls in the levels of NF subunits tended to be greater compared to those of the other cytoskeletal proteins in all HD-exposed groups, and the trend for decrements in NF-M was greater than those in the other NF subunits. Thus, HD intoxication was associated with significant declines in cytoskeletal protein contents in rat sciatic-tibial nerves, and the decreases might be related to the involvement of the peripheral axonopathy induced by HD.
Zhang
Tianliang
T
Institute of Toxicology, Shandong University, Shandong, Jinan 250012, PR China.
Zhao
Xiulan
X
Zhu
Zhenping
Z
Yu
LiHua
L
Han
Xiaoying
X
Zhang
Cuili
C
Xie
Keqin
K
D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D006588:Hexanones / Q000494:pharmacology*; D008297:Male; D008870:Microtubules / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects; D011597:Psychomotor Performance / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*; D013979:Tibial Nerve / Q000187:drug effects / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D006588:Hexanones; D014404:Tubulin; C011269:2,5-hexanedione