title: Inhibition of porcine small intestinal sucrase by valienamine.
abstract: Valienamine, an aminocyclitol, has been isolated from the enzymolysis broth of validamycins. The absolute configuration of valienamine is similar to that of alpha-D-glucose. The inhibitory effect of this amino-sugar analog of alpha-D-glucose, valienamine, on porcine small intestinal sucrase was examined. Valienamine was found to be potent, competitive reversible inhibitor of porcine small intestinal sucrase in vitro with an IC50 value of 1.17 x 10(-3)M. Valienamine also exhibited dose-dependent, instantaneous inhibition of porcine small intestinal sucrase. The inhibition of porcine small intestinal sucrase by valienamine was pH-independent.
authors.affiliation: Institute of Bioengineering, State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China. zhengyg@zjut.edu.cn
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Xu-Ping
authors.initials: XP
authors.lastname: Shentu
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000818:Animals; D053138:Cyclohexenes; D006595:Hexosamines / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology*; D013393:Sucrase / Q000037:antagonists & inhibitors*; D013552:Swine
chemical_list: D053138:Cyclohexenes; D006595:Hexosamines; C030791:valienamine; D007294:Inositol; C003749:validamycins; D013393:Sucrase