[Universal method for single nucleotide substitution identification].
A new approach to the identification of point mutations by allele-specific PCR was proposed. The mutation R408W of the human phenylalanine hydroxylase gene was used as a model. A high specificity of the approach was achieved by the use of primers partially complementary to the genomic DNA. Polyethylene glycol covalently attached to one of the allele-specific primers provides for the differential identification of the PCR products due to a change in electrophoretic mobility.
Brenner
E V
EV
Ivanova
E M
EM
Pyshnyĭ
D V
DV
Morozov
I V
IV
D000483:Alleles; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010651:Phenylalanine Hydroxylase / Q000235:genetics; D010661:Phenylketonurias / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D010651:Phenylalanine Hydroxylase