title: Repeated-batch production of kojic acid in a cell-retention fermenter using Aspergillus oryzae M3B9.
abstract: A cell-retention fermenter was used for the pilot-scale production of kojic acid using an improved strain of Aspergillus oryzae in repeated-batch fermentations. Among the various carbon and nitrogen sources used, sucrose and yeast extract promoted pellet morphology of fungi and higher kojic acid production. Repeated-batch culture using a medium replacement ratio of 75% gave a productivity of 5.3 gL(-1)day(-1) after 11.5 days of cultivation. While batch culture in shake-flasks resulted in a productivity of 5.1 gL(-1)day(-1), a productivity of 5 gL(-1)day(-1) was obtained in a pilot-scale fermenter. By converting the batch culture into repeated batches, the non-productive downtime of cleaning, filling and sterilizing the fermenter between each batch were eliminated, thereby increasing the kojic acid productivity.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Taiwan, Republic of China.
authors.forename: H M
authors.initials: HM
authors.lastname: Wan
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Giridhar
authors.forename: T S
authors.initials: TS
authors.lastname: Chang
authors.forename: W T
authors.initials: WT
authors.lastname: Wu
mesh_terms: D001236:Aspergillus oryzae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008828:Microbiological Techniques; D009584:Nitrogen / Q000378:metabolism; D011753:Pyrones / Q000378:metabolism*
chemical_list: D011753:Pyrones; C011890:kojic acid; D002244:Carbon; D009584:Nitrogen