title: [Effect of nucleolin down-regulation on the proliferation and apoptosis in C2C12 cells].
abstract: OBJECTIVE
  To clarify the effect of nucleolin on the proliferation and apoptosis in C2C12 cells.
  
  
  METHODS
  After inhibiting the expression of nucleolin using antisense oligonucleotides, the cellular proliferation was determined by MTT, and the apoptosis was detected by flow cytometry (FCM) assays and DNA ladder assays.
  
  
  RESULTS
  After being transfected with antisense oligonucleotides for 24 hours, Western blotting showed that the expression of nucleolin was repressed significantly. In cells treated with antisense oligonucleotides, the cellular proliferation was obviously inhibited; the apoptotic cell increased significantly; and the "DNA ladder" was clearly observed. But the sense and random oligonucleotides had no effect on the cellular proliferation and apoptosis.
  
  
  CONCLUSIONS
  The down-regulation of nucleolin can inhibit the cellular proliferation and initiate the apoptosis in C2C12cells.
authors.affiliation: Department of Pathophysiology , Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Kang-kai
authors.initials: KK
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Shun-mei
authors.initials: SM
authors.lastname: E
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Ling-li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Mei-dong
authors.initials: MD
authors.lastname: Liu
authors.forename: Xian-zhong
authors.initials: XZ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D051379:Mice; D032446:Myoblasts / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology*; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D000097796:Nucleolin
chemical_list: D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin