title: Antimicrobial effect of lactic acid producing bacteria culture condensate mixture (LCCM) against Salmonella enteritidis.
abstract: The antimicrobial effects of a lactic acid producing bacteria culture condensate mixture (LCCM) were assessed against Salmonella enteritidis. In the presence of LCCM, bacterial growth was assessed in vitro by the measurement of optical density (OD) and viable bacterial counting. At concentrations of 1.25 and 2.5% LCCM, OD values were significantly lower than that of the control broth, and at concentrations of 5 and 10% LCCM, OD values did not increase for the entire period of experiment. At 8 h after incubation, the viable bacterial numbers in 5% and 10% LCCM-containing broths were remarkably lower than that in the control broth. This antimicrobial ability of the LCCM was fundamentally attributed to causing cell death rather than inhibiting growth. Even when the pH of LCCM-containing broth was adjusted to 7.2, the number of viable bacteria was significantly lower in the broths containing LCCM over 2.5% than that in control broth at 8 h after incubation. However, the OD value of each culture in the presence of each concentration of the LCCM increased over 1.0 at the completion of the experiment. The in vivo antimicrobial effects of the LCCM against S. enteritidis were also assessed. In S. enteritidis-infected mice, the LCCM decreased both the viable bacteria found in the feces and the mortality rate of the mice. These findings showed that the LCCM might have an antimicrobial ability against S. enteritidis.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu, Seoul 151-742, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Seung-Hyeok
authors.initials: SH
authors.lastname: Seok
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Cho
authors.forename: Min-Won
authors.initials: MW
authors.lastname: Baek
authors.forename: Hui-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Dong-Jae
authors.initials: DJ
authors.lastname: Kim
authors.forename: Myung-June
authors.initials: MJ
authors.lastname: Chung
authors.forename: Soo-Dong
authors.initials: SD
authors.lastname: Kim
authors.forename: Un-Pyo
authors.initials: UP
authors.lastname: Hong
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005260:Female; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000096:biosynthesis / Q000494:pharmacology*; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012480:Salmonella Infections / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000254:growth & development*
chemical_list: D019344:Lactic Acid