Identification and characterization of the rat homologue of LAIR-1.
Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is a cell-surface molecule that functions as an inhibitory receptor on various immune cells in both humans and mice. We have cloned a LAIR-1 homologue from the rat that we have named rat LAIR-1. The LAIR-1 gene maps to rat chromosome 1q12 in a region showing conserved synteny with human chromosome 19q13.4 and mouse chromosome 7, where the leukocyte receptor cluster is located. Rat LAIR-1 shows 40 and 71% protein sequence identity with human LAIR-1 and mouse LAIR-1, respectively, has a single Ig-like domain and contains two immunoreceptor tyrosine-based inhibitory motif-like sequences in its cytoplasmic tail. Soluble rat LAIR-1 fusion proteins bind to the same adherent cell lines as human LAIR-1 and mouse LAIR-1, indicating that a putative ligand for all the LAIR-1 molecules is expressed on these cells. Furthermore, we show that rat and mouse LAIR-1 bind the same molecule expressed on human HT29 cells. Since many autoimmune diseases are studied in rat models, identification of rat LAIR-1 allows for in vivo studies on the function of LAIR molecules in these systems.
Lebbink
Robert Jan
RJ
Department of Immunology, University Medical Center Utrecht, Rm KC02.085.2, Lundlaan 6, 3584 EA, Utrecht, The Netherlands.
de Ruiter
Talitha
T
Kaptijn
Guido J A
GJ
Meyaard
Linde
L
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D006801:Humans; D007107:Immune System / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D008024:Ligands; D011971:Receptors, Immunologic; C108073:leukocyte-associated immunoglobulin-like receptor 1