Analytical ultracentrifugation for characterizing nanocrystals and their bioconjugates.
Analytical ultracentrifugation (AU) provides a general way to probe the polydispersity of nanoparticles and the formation of bioconjugates in solution. Unconjugated gold nanocrystals show sedimentation coefficient distributions that are in agreement with size distributions as measured by TEM. AU is sensitive to the size/shape changes elicited by conjugation, in this case to lactose repressor (LacI). AU data reveal saturating protein concentrations for conjugates that correspond to the measured stoichiometry of the complex under these conditions.
Calabretta
Michelle
M
The Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
Jamison
Jennifer A
JA
Falkner
Joshua C
JC
Liu
Yunping
Y
Yuhas
Benjamin D
BD
Matthews
Kathleen S
KS
Colvin
Vicki L
VL
D000465:Algorithms; D020099:Coated Materials, Biocompatible / Q000032:analysis* / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D006046:Gold / Q000737:chemistry; D049329:Nanostructures / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012097:Repressor Proteins / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure; D014461:Ultracentrifugation / Q000379:methods*
D020099:Coated Materials, Biocompatible; D012097:Repressor Proteins; D006046:Gold