Successful transplantation of hepatocytes requires temporary elimination of scavenger and NK cells, partial hepatectomy and ligation of bile duct.
Isolated syngeneic and allogeneic hepatocytes transplanted to spleen parenchyma are rapidly disintegrated. Host scavenger monocytes and macrophages are responsible for this reaction. We designed a method overcoming early disintegration of the intra-splenic grafted hepatocytes. It consisted of administration of anti-asialoGM I antiserum eliminating NK cells, sublethal whole body irradiation for depletion of scavenging cells and reconstitution with syngeneic bone marrow cells, ligation of host bile duct, intrasplenic hepatocyte transplantation and 3 consecutive partial hepatectomies. Six months after transplantation a glycogen-rich, trabeculae-forming, dividing hepatocytes, situated along strands of newly-formed fibrous tissue and numerous dilated blind bile canaliculae were observed. There was evidently more bile canaliculae in hosts with ligated bile duct than non-ligated controls. This is the first study showing fibrous tissue formed at the site of hepatocyte implantation and stellate cells are presumably responsible for this process.
Wesolowska
Anna
A
Department of Surgical Research and Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland.
Gewartowska
Magdalena
M
Olszewski
Waldemar L
WL
Durlik
Marek
M
D000818:Animals; D001648:Bile Canaliculi / Q000166:cytology / Q000502:physiology*; D001652:Bile Ducts / Q000601:surgery*; D006003:Glycogen / Q000378:metabolism; D006085:Graft Survival; D006498:Hepatectomy; D022781:Hepatocytes / Q000637:transplantation*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D008026:Ligation; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000502:physiology
D006003:Glycogen