In vitro screening of probiotic properties of Saccharomyces cerevisiae var. boulardii and food-borne Saccharomyces cerevisiae strains.
The probiotic potential of 18 Saccharomyces cerevisiae strains used for production of foods or beverages or isolated from such, and eight strains of Saccharomyces cerevisiae var. boulardii, was investigated. All strains included were able to withstand pH 2.5 and 0.3% Oxgall. Adhesion to the nontumorigenic porcine jejunal epithelial cell line (IPEC-J2) was investigated by incorporation of 3H-methionine into the yeast cells and use of liquid scintillation counting. Only few of the food-borne S. cerevisiae strains exhibited noteworthy adhesiveness with the strongest levels of adhesion (13.6-16.8%) recorded for two isolates from blue veined cheeses. Merely 25% of the S. cerevisiae var. boulardii strains displayed good adhesive properties (16.2-28.0%). The expression of the proinflammatory cytokine IL-1alpha decreased strikingly in IPEC-J2 cells exposed to a Shiga-like toxin 2e producing Escherichia coli strain when the cells were pre- and coincubated with S. cerevisiae var. boulardii even though this yeast strain was low adhesive (5.4%), suggesting that adhesion is not a mandatory prerequisite for such a probiotic effect. A strain of S. cerevisiae isolated from West African sorghum beer exerted similar effects hence indicating that food-borne strains of S. cerevisiae may possess probiotic properties in spite of low adhesiveness.
van der Aa Kühle
Alis
A
Department of Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark. aak@kvl.dk
Skovgaard
Kerstin
K
Jespersen
Lene
L
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002460:Cell Line; D018920:Coculture Techniques; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism; D008403:Mass Screening; D019936:Probiotics; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013552:Swine
D007375:Interleukin-1