title: Aminopeptidase N/CD13 targeting fluorescent probes: synthesis and application to tumor cell imaging.
abstract: A family of fluorescein-peptide conjugates (CNP1-3) for aminopeptidase N (APN/CD13) targeting fluorescent probes were designed and synthesized. Among the three conjugates, CNP1 bearing tumor-homing cyclic peptide CNGRC, could selectively label APN/CD13 over-expressing on the surface of tumor cells of HT-1080, as identified by means of fluorescent microscopic cell imaging. CNP1 was shown to be a promising fluorescent probe applicable to tumor-targeting molecular imaging.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. zhouen@astem.or.jp
authors.forename: Zhouen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Harada
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hatta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hiraoka
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Nishimoto
mesh_terms: D018826:CD13 Antigens / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D018826:CD13 Antigens