title: Nonpreserved human amniotic membrane transplantation for conjunctival reconstruction after excision of extensive ocular surface neoplasia.
abstract: OBJECTIVE
  To report our experience on the use of nonpreserved human amniotic membrane transplantation (AMT) in ocular surface reconstruction after excision of extensive ocular surface neoplasia (OSN).
  
  
  METHODS
  Prospective noncomparative interventional case series.
  
  
  METHODS
  In all, 10 eyes of 10 consecutive patients with extensive OSN involving various areas of limbus, conjunctiva, and cornea (conjunctival carcinoma in situ, four eyes; squamous cell carcinoma, three eyes; malignant melanoma, two eyes; conjunctival-orbital lymphangioma, one eye) were included in this prospective noncomparative interventional case series. After excision of the neoplasia with 3-4 mm tumour-free margins, double freeze-thaw cryotherapy was applied to the margins of the remaining conjunctiva, and nonpreserved human amniotic membrane graft was sutured to the adjacent conjunctiva using 8/0 vicryl sutures and cornea using 10/0 nylon sutures, with the epithelial side facing up to cover the bare sclera and cornea. Postoperatively, topical corticosteroids were used for 3 months.
  
  
  RESULTS
  After tumour excision and AMT, a satisfactory result with a wet, stable conjunctiva, and rapid and complete healing was observed in all eyes. Over a mean follow-up of 10.0 months (range, 6-27 months), all but one eye remained free of tumour recurrence. In one eye with conjunctival melanoma, there was a small recurrence, which was treated with excision and cryotherapy. Treatment complications were partial stem cell deficiency in two eyes and symblepharon formation in one eye. Immune graft rejection was not encountered.
  
  
  CONCLUSIONS
  Nonpreserved human AMT appears to be useful for reconstruction of ocular surface following excision of extensive OSN.
authors.affiliation: Department of Ophthalmology, Ankara University Faculty of Medicine, Turkey. eyemd@ada.net.tr
authors.forename: K
authors.initials: K
authors.lastname: Gündüz
authors.forename: O O
authors.initials: OO
authors.lastname: Uçakhan
authors.forename: A
authors.initials: A
authors.lastname: Kanpolat
authors.forename: I
authors.initials: I
authors.lastname: Günalp
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion / Q000637:transplantation*; D003228:Conjunctiva / Q000601:surgery*; D003230:Conjunctival Neoplasms / Q000473:pathology / Q000601:surgery*; D003315:Cornea / Q000473:pathology / Q000601:surgery; D003452:Cryosurgery; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011446:Prospective Studies; D016896:Treatment Outcome; D014792:Visual Acuity