title: Self-association of glutamic acid-rich fusion peptide analogs of influenza hemagglutinin in the membrane-mimic environments: effects of positional difference of glutamic acids on side chain ionization constant and intra- and inter-peptide interactions deduced from NMR and gel electrophoresis measurements.
abstract: Two glutamic acid-rich fusion peptide analogs of influenza hemagglutinin were synthesized to study the organization of the charged peptides in the membranous media. Fluorescence and gel electrophoresis experiments suggested a loose association between the monomers in the vesicles. A model was built which showed that a positional difference of 3, 7 and 4, 8 results in the exposure of Glu3 and Glu7 side chains to the apolar lipidic core. Supportive results include: first, pK(a) values of two pH units higher than reference value in aqueous medium for Glu3 and Glu7 CgammaH, whereas the deviation of pK(a) from the reference value for Glu4 and Glu8 CgammaH is substantially smaller; second, Hill coefficients of titration shift of these protons indicate anti-cooperativity for Glu3 and Glu7 side chain protons but less so for Glu4 and Glu8, implying a strong electrostatic interaction between Glu3 and Glu7 possibly resulting from their localization in an apolar environment; third, positive and larger titration shift for NH of Glu3 is observed compared to that of Glu4, suggesting stronger hydrogen bond between the NH and the carboxylic group of Glu3 than that of Glu4, consistent with higher degree of exposure to hydrophobic medium for the side chain of Glu3.
authors.affiliation: Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China. dkc@chem.sinica.edu.tw
authors.forename: Ding-Kwo
authors.initials: DK
authors.lastname: Chang
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Cheng
authors.forename: Chi-Hui
authors.initials: CH
authors.lastname: Lin
authors.forename: Ericassen B
authors.initials: EB
authors.lastname: Kantchev
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Wu
mesh_terms: D002210:Caprylates / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D019281:Dimerization; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer; D005466:Fluorocarbons / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry*; D006388:Hemagglutinins / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000378:metabolism; D007477:Ions; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011522:Protons; D011994:Recombinant Proteins / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D055672:Static Electricity
chemical_list: D002210:Caprylates; D005466:Fluorocarbons; D006388:Hemagglutinins; D007477:Ions; D008055:Lipids; D010455:Peptides; D010715:Phosphatidylglycerols; D011522:Protons; D011994:Recombinant Proteins; D012235:Rhodamines; D012967:Sodium Dodecyl Sulfate; D003035:Cobalt; D000431:Ethanol; D018698:Glutamic Acid; C023036:perfluorooctanoic acid; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine