Changes of osteocalcin and IGF-I during bone lengthening.
OBJECTIVE
To examine the changes of osteocalcin and insulin-like growth factor I (IGF-I) during bone lengthening, and to clarify the mechanism of bone healing.


METHODS
Thirty-two shepherd dogs were divided into five groups randomly. Their tibiae were lengthened by Ilvzarov's external fixator at the rate of 1 mm/day. The lengthening area was the experimental side and the opposite side was the control. Samples were obtained on the 2nd, 4th, 6th, 8th, 12th weekend respectively. The samples were defatted, dried, powdered, centrifuged and measured by radioimmunoassay.


RESULTS
The osteocalcin concentration increased at the subsequent periods, but it was significantly lower in the experimental side than that of the control side, P<0.05 and the IGF-I concentration was not significantly lowered.


CONCLUSIONS
Different noncollagenous bone growth factors may be different at different stage.
Wang
Bing
B
Department of Orthopedics, 12th Hospital of Guangzhou, Guangdong 510620, China. wbing222@163.com
Liu
Yi-yun
YY
Zheng
Jie-bai
JB
Yuan
Shi-xiang
SX
Chen
Guo-xun
GX
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001852:Bone Lengthening / Q000379:methods*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism*; D008297:Male; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism*; D011336:Probability; D011863:Radioimmunoassay; D011897:Random Allocation; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013977:Tibia / Q000601:surgery*
D015415:Biomarkers; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I