title: Contact stimulation of prostate cancer cell migration: the role of gap junctional coupling and migration stimulated by heterotypic cell-to-cell contacts in determination of the metastatic phenotype of Dunning rat prostate cancer cells.
abstract: BACKGROUND
  Motile activity of tumour cells is regarded as a critical factor determining their metastatic potential. We have shown previously that contrary to the majority of normal cells, homotypic contacts between some tumour cells, among them low metastatic (AT-2) and highly metastatic (MAT-LyLu) rat prostate cancer cells, increase the speed of their movements. The aim of the present study was to determine the effect of heterotypic cell-to-cell contacts on the migration of rat prostate cancer cells differing in metastatic potential, and to correlate it with the intensity of homo- and heterologous gap junctional coupling.
  
  
  RESULTS
  MAT-LyLu and AT-2 cells moving on the surface of fibroblasts displayed significantly greater cell displacement than those moving on plastic substrata. This effect correlated with the polarization (contact guidance) and increased speed of cell movements. However, in contrast with the migration on plastic substrata, where MAT-LyLu cells displayed considerably higher motility than AT-2 cells, no differences between both cell lines were observed on the surface of fibroblasts. On the other hand, in contrast with AT-2, Mat-LyLu cells displayed extensive homologous coupling mediated by connexin43 and were able to couple with normal fibroblasts.
  
  
  CONCLUSIONS
  Heterotypic contacts between migrating prostatic cancer cells and normal fibroblasts can strongly stimulate their migration during invasion; however, this effect does not correlate with the gap junctional coupling between cancer cells and normal fibroblasts.
authors.affiliation: Department of Cell Biology, Faculty of Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Miekus
authors.forename: Marta
authors.initials: M
authors.lastname: Czernik
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sroka
authors.forename: Jarosław
authors.initials: J
authors.lastname: Czyz
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Madeja
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D017629:Gap Junctions / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D008297:Male; D009362:Neoplasm Metastasis; D010641:Phenotype; D010969:Plastics; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D051381:Rats
chemical_list: D018031:Connexin 43; D010969:Plastics