Sensing DNA hybridization via ionic conductance through a nanoporous electrode.
We show that nanoporous alumina modified with covalently linked DNA can be used to detect target DNA by monitoring the increase in impedance at the electrode upon DNA hybridization, which resulted from blocking the pores to ionic flow. Using cyclic voltammetry, direct current conductance, and impedance spectroscopy we confirm the importance of pore size: the effect is observed with 20-nm-diameter pores and is absent for 200-nm pores.
Vlassiouk
Ivan
I
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
Takmakov
Pavel
P
Smirnov
Sergei
S
D000535:Aluminum / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D007477:Ions / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012680:Sensitivity and Specificity
D007477:Ions; D004247:DNA; D000535:Aluminum