title: Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins.
abstract: Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.
authors.affiliation: National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09, Atlanta, GA 30333, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
authors.forename: Lia M
authors.initials: LM
authors.lastname: Haynes
authors.forename: Deborah
authors.initials: D
authors.lastname: Moore
authors.forename: Barbara
authors.initials: B
authors.lastname: Anderson
authors.forename: Azaibi
authors.initials: A
authors.lastname: Tamin
authors.forename: Brian H
authors.initials: BH
authors.lastname: Harcourt
authors.forename: Les P
authors.initials: LP
authors.lastname: Jones
authors.forename: Mamadi
authors.initials: M
authors.lastname: Yilla
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Babcock
authors.forename: Thomas
authors.initials: T
authors.lastname: Greenough
authors.forename: Donna M
authors.initials: DM
authors.lastname: Ambrosino
authors.forename: Rene
authors.initials: R
authors.lastname: Alvarez
authors.forename: Justin
authors.initials: J
authors.lastname: Callaway
authors.forename: Sheana
authors.initials: S
authors.lastname: Cavitt
authors.forename: Kurt
authors.initials: K
authors.lastname: Kamrud
authors.forename: Harold
authors.initials: H
authors.lastname: Alterson
authors.forename: Jonathan
authors.initials: J
authors.lastname: Smith
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Harcourt
authors.forename: Congrong
authors.initials: C
authors.lastname: Miao
authors.forename: Raj
authors.initials: R
authors.lastname: Razdan
authors.forename: James A
authors.initials: JA
authors.lastname: Comer
authors.forename: Pierre E
authors.initials: PE
authors.lastname: Rollin
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: Anthony
authors.initials: A
authors.lastname: Sanchez
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rota
authors.forename: William J
authors.initials: WJ
authors.lastname: Bellini
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D002522:Chlorocebus aethiops; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018604:Epitope Mapping; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology*; D000086082:Viroporin Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; C501689:E protein, SARS coronavirus; C000706237:M protein, SARS-CoV; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins; D000086082:Viroporin Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus