Biosynthesis of conjugatable saccharidic moieties of GM2 and GM3 gangliosides by engineered E. coli.
Oligosaccharidic moieties of GM(2) and GM(3) gangliosides bearing an allyl or a propargyl aglycon, are efficiently biosynthesized on the gram scale by growing metabolically engineered Escherichia coli cells in the presence of the corresponding lactoside acceptors and sialic acid.
Fort
Sébastien
S
Centre de Recherches sur les Macromolécules Végétales, CERMAV-FR-CNRS 2607, affiliated to Université Joseph Fourier Grenoble, BP 53, 38041 Grenoble Cedex 9, France. Sebastien.Fort@cermav.cnrs.fr
Birikaki
Lemonia
L
Dubois
Marie-Pierre
MP
Antoine
Tatiana
T
Samain
Eric
E
Driguez
Hugues
H
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005678:G(M2) Ganglioside / Q000096:biosynthesis* / Q000737:chemistry; D005679:G(M3) Ganglioside / Q000096:biosynthesis* / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods; D006001:Glycoconjugates / Q000096:biosynthesis* / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry
D005679:G(M3) Ganglioside; D006001:Glycoconjugates; D006027:Glycosides; D009844:Oligosaccharides; C008245:lactosides; D005678:G(M2) Ganglioside; D019158:N-Acetylneuraminic Acid