Growth hormone increases hsc70/hsp70 expression and protects against apoptosis in whole blood preparations from silver sea bream.
Preparations of whole blood, from silver sea bream (Sparus sarba), were used for heat shock protein 70 (hsp70) and apoptosis studies. It was found that the expression of both gene members of the hsp70 family (hsc70 and hsp70) were upregulated during acute heat shock. The transcript abundance of both of these genes was increased when cells were exposed to growth hormone (GH) at concentrations of 10 and 100 ng/mL. It was also found that GH at concentrations of 10 and 100 ng/mL could protect whole blood from camptothecin-induced apoptosis as determined by DNA fragmentation analysis.
Deane
Eddie E
EE
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
Woo
Norman Y S
NY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D013006:Growth Hormone / Q000494:pharmacology / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D021541:Sea Bream / Q000097:blood* / Q000235:genetics
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D013006:Growth Hormone