[Measurement of monoamine transmitters in the brains of ovariectomized mice using high performance liquid chromatography with fluorescence detection and gradient flow rate elution].
A method for the determination of norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in brains of ovariectomized mice was established by using high performance liquid chromatograph with a fluorescence detector, a C18 column and a gradient flow rate delivered by a single pump. The mobile phase was a mixture of 0.1 mol/L KH2PO4 and CH3OH (9/1, v/v). Detection limits of the method were determined to be lower than 10 nmol/L, absolute recoveries were above 80% and the relative standard deviations (RSDs) of intra-day were all within 3.03%. The method has the advantages of high sensitivity and of simple sample preparation. Short analysis time and better separation of samples were also obtained.
Zhang
Lei
L
Department of Biomedical Engineering of Zhejiang University, Hangzhou 310027, China. zl78213@yahoo.com.cn
Lu
Yingjun
Y
Deng
Tongle
T
Zheng
Xiaoxiang
X
D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D001921:Brain / Q000378:metabolism*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis; D005260:Female; D005453:Fluorescence; D006112:Gravitation; D051379:Mice; D009638:Norepinephrine / Q000032:analysis; D010052:Ovariectomy; D012701:Serotonin / Q000032:analysis
D015306:Biogenic Monoamines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine