A fungal tRNA of Aspergillus niger induces IFN-beta synthesis in HEp-2 cells.
In this work, we evaluated the capacity of a fungal transfer RNA (F-tRNA) from Aspergillus niger to protect HEp-2 cells against a viral infection, and as an inducer of IFN-beta synthesis. HEp-2 cells previously incubated with F-tRNA, polyI:polyC, or IFN-alpha, at different concentrations for 24 h were infected with 200 pfu of adenovirus type 6 (AdV-6); after 5 days, we determined cellular viability, cytopathic effect of the virus, optimal concentration necessary to inhibit the cytopathic effect, and IFN-beta expression by RT-PCR. Results showed that HEp-2 cells treated with F-tRNA were less susceptible to the cytopathic effect of AdV-6 infection than those incubated with polyI:polyC (p < 0.05). On the other hand, F-tRNA- treated HEp-2 cells expressed IFN-beta mRNA, whereas monolayers incubated with polyI:polyC or IFN-alpha did not. Our results suggest that F-tRNA protected HEp-2 cells against AdV-6 infection, due to its capacity to induce IFN-beta synthesis.
Alvarado-Vásquez
Noé
N
Departamento de Bioquímica Instituto Nacional de Enfermedades Respiratorias. Calz. de Tlalpan 4502, Col Sección XVI, C.P 14080, Mexico. nnooee@salud.gop.mx
Santiago
Julio
J
Alcázar-Leyva
Susana
S
Zenteno
Edgar
E
Negrete-García
Cristina
C
Alcázar-Montenegro
Heberto
H
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000256:Adenoviridae; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000235:genetics*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000494:pharmacology*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
D000998:Antiviral Agents; D012333:RNA, Messenger; D012367:RNA, Viral; D016899:Interferon-beta; D012343:RNA, Transfer; D011070:Poly I-C