title: Effect of ischemic preconditioning on reactive oxygen species-mediated ischemia--reperfusion injury in the isolated perfused rat lung.
abstract: OBJECTIVE
  To investigate the protective effect of ischemic preconditioning (IP) in the early phase of reperfusion injury.
  
  
  METHODS
  Control rat lungs were subjected to 3 h of perfusion, whereas the lungs of the ischemia-then-reperfusion (I/R) group were subjected to 2 h of cold ischemia following 30 min of perfusion. IP was performed by two cycles of 5-min ischemia followed by 5 min of reperfusion prior to 2-h cold ischemia. Lipid peroxidation and antioxidant status were determined in tissue samples.
  
  
  RESULTS
  Lipid peroxidation and reduced/oxidized glutathione (GSH/GSSG) ratio were increased; antioxidant enzyme activities were decreased in the I/R group whereas lipid peroxidation and GSH/GSSG ratio were decreased; antioxidant enzyme activities were increased in the IP group.
  
  
  CONCLUSIONS
  IP appeared to have a protective effect against reactive oxygen species-mediated I/R injury in isolated rat lung.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey. gulberk@hacepette.edu.tr
authors.forename: Gülberk
authors.initials: G
authors.lastname: Ucar
authors.forename: Eda
authors.initials: E
authors.lastname: Topaloglu
authors.forename: H Burak
authors.initials: HB
authors.lastname: Kandilci
authors.forename: Bülent
authors.initials: B
authors.lastname: Gümüsel
mesh_terms: D000818:Animals; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D019194:Ischemic Preconditioning; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D010544:Peroxidases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D017382:Reactive Oxygen Species; D010544:Peroxidases; D005978:Glutathione