title: Landscape phage probes for Salmonella typhimurium.
abstract: We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.
authors.affiliation: Department of Pathobiology, Auburn University, 252 Greene Hall, College of Veterinary Medicine, Auburn, AL 36849, USA.
authors.forename: Iryna B
authors.initials: IB
authors.lastname: Sorokulova
authors.forename: Eric V
authors.initials: EV
authors.lastname: Olsen
authors.forename: I-Hsuan
authors.initials: IH
authors.lastname: Chen
authors.forename: Ben
authors.initials: B
authors.lastname: Fiebor
authors.forename: James M
authors.initials: JM
authors.lastname: Barbaree
authors.forename: Vitaly J
authors.initials: VJ
authors.lastname: Vodyanoy
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Chin
authors.forename: Valery A
authors.initials: VA
authors.lastname: Petrenko
mesh_terms: D005456:Fluorescent Dyes; D008828:Microbiological Techniques / Q000379:methods*; D019151:Peptide Library; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D019151:Peptide Library