Vitamin E deficiency reduced lumbar bone calcium content in female rats.
Vitamin E deficiency has been found to impair bone calcification. This study was done to determine the effects of vitamin E deficiency and supplementation on parathyroid hormone, i.e. the hormone involved in bone regulation. Female Sprague-Dawley rats were divided into 4 groups: 1) normal rat chow (RC), 2) vitamin E deficiency (VED), vitamin E deficient rats supplemented with 3) 60 mg/kg alpha-tocotrienol (ATT) and 4) 60 mg/kg (alpha-tocopherol (ATF). Treatment was carried out for 3 months. Vitamin E deficiency caused hypocalcaemia during the first month of the treatment period, increased the parathyroid hormone level in the second month and decreased the bone calcium content in the 4th lumbar bone at the end of the treatment. Vitamin E supplementation (ATT and ATF) failed to improve these conditions. The bone formation marker, osteocalcin, and the bone resorption marker, deoxypyridinoline did not change throughout the study period. In conclusion vitamin E deficiency impaired bone calcium homeostasis with subsequent secondary hyperparathyroidism and vertebral bone loss. Replacing the vitamin E with pure ATF or pure ATT alone failed to correct the changes seen.
Norazlina
M
M
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Chua
C W
CW
Ima-Nirwana
S
S
D000596:Amino Acids / Q000652:urine; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002118:Calcium / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D019587:Dietary Supplements; D005260:Female; D008159:Lumbar Vertebrae / Q000378:metabolism*; D015675:Osteocalcin / Q000097:blood; D010281:Parathyroid Hormone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D024508:Tocotrienols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D014811:Vitamin E Deficiency / Q000188:drug therapy / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000627:therapeutic use*
D000596:Amino Acids; D000975:Antioxidants; D010281:Parathyroid Hormone; D024508:Tocotrienols; D015675:Osteocalcin; D014810:Vitamin E; C036020:deoxypyridinoline; D003404:Creatinine; C082032:tocotrienol, alpha; D024502:alpha-Tocopherol; D002118:Calcium