Landscape phage probes for Salmonella typhimurium.
We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.
Sorokulova
Iryna B
IB
Department of Pathobiology, Auburn University, 252 Greene Hall, College of Veterinary Medicine, Auburn, AL 36849, USA.
Olsen
Eric V
EV
Chen
I-Hsuan
IH
Fiebor
Ben
B
Barbaree
James M
JM
Vodyanoy
Vitaly J
VJ
Chin
Bryan A
BA
Petrenko
Valery A
VA
D005456:Fluorescent Dyes; D008828:Microbiological Techniques / Q000379:methods*; D019151:Peptide Library; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D019151:Peptide Library