Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda.
Four Gram-negative, non-spore-forming, slightly halophilic rods (strains SW-62(T), SW-74, SW-63(T) and SW-72) with appendages were isolated from a salt lake near Hwajinpo Beach on the East Sea in Korea, and subjected to a polyphasic taxonomic analysis. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SW-62(T), SW-74, SW-63(T) and SW-72 formed a coherent cluster with Muricauda ruestringensis. Strains SW-62(T) and SW-74 had the same 16S rRNA gene sequence, as did strains SW-63(T) and SW-72. The level of 16S rRNA gene sequence similarity between strains SW-62(T) and SW-63(T) was 97.0 %. Strains SW-62(T) and SW-63(T) exhibited 16S rRNA gene similarity levels of 96.5 and 98.3 %, respectively, with respect to M. ruestringensis DSM 13258(T). The predominant isoprenoid quinone found in the four isolates and M. ruestringensis DSM 13258(T) was MK-6. The four strains contained iso-C(17 : 0) 3-OH, iso-C(15 : 1) and iso-C(15 : 0) as the major fatty acids. Their DNA G + C contents were 44.1-45.4 mol%. The levels of DNA-DNA relatedness indicated that strains SW-62(T) and SW-74 and strains SW-63(T) and SW-72 were members of two species that were different from M. ruestringensis. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains SW-62(T) and SW-74 and strains SW-63(T) and SW-72 were placed in the genus Muricauda as two distinct novel species, for which the names Muricauda flavescens sp. nov. (type strain, SW-62(T) = KCCM 41645(T) = JCM 11812(T)) and Muricauda aquimarina sp. nov. (type strain, SW-63(T) = KCCM 41646(T) = JCM 11811(T)), respectively, are proposed.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Lee
Mi-Hwa
MH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Park
Yong-Ha
YH
National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041965:Flavobacteriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D005840:Gentian Violet; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; C042455:menaquinone 6; D005840:Gentian Violet