Inhibitory effect of the tea polyphenol, (-)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines.
To investigate the effect of (-)-epigallocatechin gallate (EGCG) on cervical adenocarcinoma, we performed a cell proliferation assay. TRAP assay is used for telomerase activity, flow cytometry analysis and pKi-67 immunofluoroscein staining in cervical adenocarcinoma cell lines (OMC-4, TMCC-1). Our results showed that EGCG inhibited the proliferation assay, TRAP assay for telomerase activity of both cell lines. Although cell apoptosis was induced, we observed that the expression of pKi-67 was suppressed. Our data suggest that EGCG may be effective for the treatment of cervical adenocarcinoma. The mechanisms of the anti-tumor effects were revealed to be the inhibition of telomerase activity, the induction of apoptosis and cell cycle dysregulation.
Noguchi
Mitsuyo
M
Department of Obstetrics and Gynecology, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
Yokoyama
Masatoshi
M
Watanabe
Sumiko
S
Uchiyama
Michiko
M
Nakao
Yoshifumi
Y
Hara
Kouichi
K
Iwasaka
Tsuyoshi
T
D000230:Adenocarcinoma / Q000188:drug therapy*; D017209:Apoptosis / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005419:Flavonoids; D005434:Flow Cytometry; D006801:Humans; D019394:Ki-67 Antigen; D010636:Phenols; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*; D019098:Telomerase / Q000096:biosynthesis / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
D005419:Flavonoids; D019394:Ki-67 Antigen; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D019098:Telomerase