title: A disposable electrochemical sensor for the rapid determination of levodopa.
abstract: Levodopa (L-dopa), the biological precursor of catecholamines, is the most widely prescribed drug in the treatment of Parkinson's disease. The present work presents a proposal for the application of a gold screen-printed electrode an electrochemical sensor for monitoring L-dopa in stationary solution and a flow system. Using the electrooxidation of L-dopa at +0.63 V in acetate buffer pH 3.0 on a gold screen-printed electrode it is possible to obtain a linear calibration curve from 9.9 x 10(-5) to 1.2 x 10(-3) mol L(-1) and a detection limit of 6.8 x 10(-5) mol L(-1). Under amperometric conditions (E(app) = 0.8 V; flow rate = 14.1 mL min(-1); pH 3.0), an analytical calibration graph for l-dopa was obtained from 1.0 x 10(-6) mol L(-1) 6.6 x 10(-4) mol L(-1) with a detection limit of 9.9 x 10(-7) mol L(-1). The method was successfully applied to the determination of L-dopa in commercial dosage forms without any pre-treatment.
authors.affiliation: Departamento de Química Analítica, Instituto de Química, Universidade Estadual Paulista, UNESP, CP 355, Araraquara, SP, Brazil.
authors.forename: Márcio F
authors.initials: MF
authors.lastname: Bergamini
authors.forename: André L
authors.initials: AL
authors.lastname: Santos
authors.forename: Nelson R
authors.initials: NR
authors.lastname: Stradiotto
authors.forename: Maria Valnice B
authors.initials: MV
authors.lastname: Zanoni
mesh_terms: D015259:Dopamine Agents / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D007980:Levodopa / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry
chemical_list: D015259:Dopamine Agents; D004364:Pharmaceutical Preparations; D007980:Levodopa