Aminopeptidase N/CD13 targeting fluorescent probes: synthesis and application to tumor cell imaging.
A family of fluorescein-peptide conjugates (CNP1-3) for aminopeptidase N (APN/CD13) targeting fluorescent probes were designed and synthesized. Among the three conjugates, CNP1 bearing tumor-homing cyclic peptide CNGRC, could selectively label APN/CD13 over-expressing on the surface of tumor cells of HT-1080, as identified by means of fluorescent microscopic cell imaging. CNP1 was shown to be a promising fluorescent probe applicable to tumor-targeting molecular imaging.
Zhang
Zhouen
Z
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. zhouen@astem.or.jp
Harada
Hiroshi
H
Tanabe
Kazuhito
K
Hatta
Hiroshi
H
Hiraoka
Masahiro
M
Nishimoto
Sei-ichi
S
D018826:CD13 Antigens / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D018826:CD13 Antigens