title: A histological evaluation for guided bone regeneration induced by a collagenous membrane.
abstract: This study was designed to evaluate the histological changes during ossification and cellular events including osteogenic differentiation responding to collagenous bioresorbable membranes utilized for GBR. Standardized artificial bony defects were prepared at rat maxillae, and covered with a collagenous bioresorbable membrane. These animals were sacrificed at 1, 2, 3 and 4 weeks after the GBR-operation. The paraffin sections were subject to tartrate resistant acid phosphatase (TRAP) enzyme histochemistry and immunohistochemistry for alkaline phosphatase (ALP), osteopontin (OP) and osteocalcin (OC). In the first week of the experimental group, woven bone with ALP-positive osteoblasts occupied the lower half of the cavity. The collagenous membrane included numerous ALP-negative cells and OP-immunoreactive extracellular matrices. At 2 weeks, the ALP-, OP- and OC-immunoreactivity came to be recognizable in the region of collagenous membrane. Since ALP-negative soft tissue separated the collagenous membrane and the new bone originating from the cavity bottom, the collagenous membrane appeared to induce osteogenesis in situ. At 3 weeks, numerous collagen fibers of the membrane were embedded in the adjacent bone matrix. At 4 weeks, the membrane-associated and the cavity-derived bones had completely integrated, showing the same height of the periosteal ridge as the surrounding alveolar bones. The collagen fibers of a GBR-membrane appear to participate in osteogenic differentiation.
authors.affiliation: Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. yuya@dent.niigata-u.ac.jp
authors.forename: Yuya
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Nakadate
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Noritaka
authors.initials: N
authors.lastname: Fujii
authors.forename: Kimimitsu
authors.initials: K
authors.lastname: Oda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nomura
authors.forename: Takeyasu
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D001861:Bone Regeneration / Q000502:physiology*; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry / Q000627:therapeutic use*; D017102:Fracture Healing / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D008422:Materials Testing; D008440:Maxillary Fractures / Q000473:pathology* / Q000628:therapy*; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000473:pathology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: C106416:Bio-Gide; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D003094:Collagen