Nanogel-quantum dot hybrid nanoparticles for live cell imaging.
We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.
Hasegawa
Urara
U
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Nomura
Shin-ichiro M
SM
Kaul
Sunil C
SC
Hirano
Takashi
T
Akiyoshi
Kazunari
K
D002240:Carbohydrate Sequence; D002477:Cells; D002784:Cholesterol / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D008969:Molecular Sequence Data; D036103:Nanotechnology; D010316:Particle Size
D005936:Glucans; C009109:pullulan; D002784:Cholesterol