Isolation and characterization of a new Vesivirus from rabbits.
This report describes the isolation, cDNA cloning, complete genome nucleotide sequence, and partial characterization of a new cultivable calicivirus isolated from juvenile feeder European rabbits (Oryctolagus cuniculus) showing symptoms of diarrhea. Absence of neutralization by type-specific neutralizing antibodies for 40 caliciviruses and phylogenetic sequence comparisons of the open reading frame 1-encoded polyprotein with those of other caliciviruses demonstrate that this new calicivirus is a putative novel member of the Vesivirus genus which is closely related to the marine calicivirus subgroup. According to its putative classification, this new virus has been named rabbit vesivirus.
Martín-Alonso
José M
JM
Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Edificio Santiago Gascón, Campus El Cristo, Universidad de Oviedo, 33006 Oviedo, Spain.
Skilling
Douglas E
DE
González-Molleda
Lorenzo
L
del Barrio
Gloria
G
Machín
Angeles
A
Keefer
Nathan K
NK
Matson
David O
DO
Iversen
Patrick L
PL
Smith
Alvin W
AW
Parra
Francisco
F
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits / Q000821:virology*; D029342:Vesivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral