Single inner cell masses yield embryonic stem cell lines differing in lifr expression and their developmental potential.
The unique differentiation potential of inner cell mass derived embryonic stem cells together with their outstanding self-renewal capacity makes them a desirable source for somatic cell therapy of human diseases. Somatic cells are gained by in vitro differentiation of embryonic stem cells, however, the differentiation potential of embryonic stem cells varied even between isogenic cell lines. Variable differentiation potentials may either be a consequence of an inherent inhomogeneity of gene expression in the inner cell mass or may have technical reasons. To understand variations in the differentiation potential, we generated pairs of isogenic, monozygotic twin, and single inner cell mass derived clonal embryonic stem cell lines, and demonstrate that they differentially express the leukaemia inhibitory factor receptor gene. Variations of leukaemia inhibitory factor receptor protein levels are already evident in the inner cell mass and predispose the cardiomyogenic potential of embryonic stem cell lines in a Janus activated kinase dependent manner. Thus, a single inner cell mass may give rise to embryonic stem cell lines with different developmental potentials.
Lauss
Martin
M
Max F. Perutz Laboratories, University Institutes at the Vienna Biocenter, Department of Medical Biochemistry, Division of Molecular Cell Biology, Medical University of Vienna, Dr. Bohrgasse 9, A1030 Vienna, Austria.
Stary
Martina
M
Tischler
Julia
J
Egger
Gerda
G
Puz
Sonja
S
Bader-Allmer
Alice
A
Seiser
Christian
C
Weitzer
Georg
G
D000818:Animals; D002319:Cardiovascular System / Q000196:embryology; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D051379:Mice; D009132:Muscles / Q000196:embryology; D018121:Receptors, Cytokine / Q000378:metabolism*; D053606:Receptors, OSM-LIF; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*
C507840:LIFR protein, human; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF