title: Functional analysis of transcriptional repressor Otx3/Dmbx1.
abstract: Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.
authors.affiliation: Division of Cellular and Molecular Medicine, Kobe University, Graduate School of Medicine, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Kimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Miki
authors.forename: Tadao
authors.initials: T
authors.lastname: Shibasaki
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Makoto
authors.initials: M
authors.lastname: Ogawa
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Saisho
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okuno
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Iwanaga
authors.forename: Susumu
authors.initials: S
authors.lastname: Seino
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D019281:Dimerization; D018398:Homeodomain Proteins / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology*; D051857:Otx Transcription Factors; D012097:Repressor Proteins / Q000737:chemistry / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D017931:DNA Primers; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051857:Otx Transcription Factors; C470836:Otx3 protein, mouse; D012097:Repressor Proteins