Dynamic study of calcium phosphate formation on porous HA/TCP ceramics.
Bone-like apatite formation on porous calcium phosphate ceramics was investigated in static simulated body fluid (SBF) and dynamic SBF at different flowing rates. The results of a 14-day immersion in static SBF showed that the formation of bone-like apatite occurred both on the surface and in the pores of the samples. When SBF flow at the physiological flow rate in muscle (2 ml/100 ml min1), bone-like apatite could be detected only in internal surface of the pores of samples. The result that bone-like apatite formation could only be found in the pores when SBF flown at physiological flow rate was consistent with that of porous calcium phosphate ceramics implanted in vivo: osteoinduction was only detected inside the pores of the porous calcium phosphate ceramics. This result implicates that the bone-like apatite may play an important role in the osteoinduction of Ca-P materials. The dynamic model used in this study may be better than usually used static immersion model in imitating the physiological condition of bone-like apatite formation. Dynamic SBF method is very useful to understand bone-like apatite formation in vivo and the mechanism of ectopic bone formation in calcium phosphate ceramics.
Duan
Y R
YR
College of Material Science and Engineering, Dong Hua University, Shanghai 20051, China.
Zhang
Z R
ZR
Wang
C Y
CY
Chen
J Y
JY
Zhang
X D
XD
D040761:Biomimetic Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D018786:Bone Substitutes / Q000032:analysis / Q000737:chemistry*; D002130:Calcium Phosphates / Q000032:analysis / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D017886:Durapatite / Q000737:chemistry; D007700:Kinetics; D008422:Materials Testing / Q000379:methods*; D044085:Microfluidics / Q000379:methods; D016062:Porosity; D013499:Surface Properties
D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D017886:Durapatite; C018392:tricalcium phosphate