Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents.
The mammalian gastrointestinal tract is covered by a layer of mucus that can harbour a range of bacterial species specifically adapted to colonize this ecological niche. Examination of 110 bacterial isolates cultivated from the gastrointestinal tract of 23 mice revealed the presence of a subgroup of 30 isolates that did not correspond genetically with genera commonly associated with this site, i.e. members of the epsilon-Proteobacteria such as Helicobacter and Campylobacter species. Instead this group of isolates was found to lie within the phylum Deferribacteres, a completely distinct lineage in the domain Bacteria. There was a high level of consensus in results obtained from the phenotypic and genotypic characterization of a number of the isolates, which showed they were distinct from other members of the Deferribacteres. As such, they are proposed to constitute a new genus and species, Mucispirillum schaedleri gen. nov., sp. nov. These organisms are anaerobic, Gram-negative, spiral-shaped rods with bipolar flagella. The type strain is HRI I17(T) (= ATCC BAA-1009(T) = ACM 5223(T)).
Robertson
Bronwyn R
BR
Ramaciotti Centre for Gene Function Analysis, The University of New South Wales, Sydney, NSW 2052, Australia.
O'Rourke
Jani L
JL
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
Neilan
Brett A
BA
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
Vandamme
Peter
P
Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium.
On
Stephen L W
SLW
Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
Fox
James G
JG
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Lee
Adrian
A
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D002167:Campylobacter / Q000145:classification / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005407:Flagella / Q000502:physiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020459:Genes, rRNA; D005840:Gentian Violet; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D016998:Helicobacter / Q000145:classification / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009093:Mucus / Q000382:microbiology*; D010619:Phenazines; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D010619:Phenazines; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet