Developmental changes in Trichoderma viride enzymes abundant in conidia and the light-induced conidiation signalling pathway.
The expression of glutamic acid decarboxylase gene and the laccase activity were measured during the development of surface-cultivated Trichoderma viride mycelia in order to examine their up-regulation by light. The results show that the changes in activity of GAD induced by light observed previously are caused by transcriptional regulation of gad gene expression in both submerged mycelia and aerial mycelia after photoinduction. The expression of tga gene encoding a T. viride G(alpha) protein was found not to be up-regulated by light and was also present in the non-conidiating mutant of T. viride suggesting that this protein is not involved in the regulation of conidiation in this fungus, or that it plays a role is in later stages of conidia development. The activity of laccase was also not light-inducible and may be related to the maturation of conidia.
Pokorný
Richard
R
Department of Biochemistry and Microbiology, Slovak University of Technology, Radlinského 9, 81237-Bratislava, Slovakia.
Vargovic
Peter
P
Hölker
Udo
U
Janssen
Martina
M
Bend
Jutta
J
Hudecová
Daniela
D
Varecka
L'udovít
L
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005968:Glutamate Decarboxylase / Q000235:genetics*; D042845:Laccase / Q000032:analysis; D008027:Light; D008969:Molecular Sequence Data; D025282:Mycelium / Q000201:enzymology; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D004271:DNA, Fungal; D044385:GTP-Binding Protein alpha Subunits; D012331:RNA, Fungal; D012333:RNA, Messenger; D042845:Laccase; D005968:Glutamate Decarboxylase