title: [Cloning and expression of a gene encoding shortened cecropin A-melittin hybrid in E.coli].
abstract: According to the studies of David Andreu and other scientists, we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid. This hybrid consists of CA(1-7) and M(5-12). The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI. A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).
authors.affiliation: Department of Biotechnology, College of Life Scienceand Technology, Jinan University, Guangzhou 510630, China. tom@jnu.edu.cn
authors.forename: Jia-ming
authors.initials: JM
authors.lastname: Zhu
authors.forename: Fei-peng
authors.initials: FP
authors.lastname: Liu
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Li
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Zhong
authors.forename: Na
authors.initials: N
authors.lastname: Zhang