Microgravimetric flow analysis of nucleic acid based on adsorption of nanoparticle-bioconjugate.
A novel nanoparticle-bioconjugate has been prepared by specific hybridization of the target with complementary thiol-labeled and nanoparticle-labeled probes. The rapid adsorption of the nanoparticle-bioconjugate onto a gold surface via a thiol-gold reaction was monitored in real-time using a quartz crystal microbalance, and used to perform microgravimetric flow analysis of nucleic acid for the first time. This innovative assay is highly reproducible and sensitive, and shows great promise for clinical applications.
Mo
Z-H
ZH
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China. zhihmo@cqu.edu.cn
Liang
Y-L
YL
Wang
H-L
HL
Liu
F-W
FW
Xue
Y-X
YX
D000327:Adsorption; D002498:Centrifugation / Q000379:methods; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D008832:Microchemistry; D008872:Microwaves; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011791:Quartz; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D011791:Quartz; D006046:Gold; D004247:DNA