title: Monosaccharide composition analysis of glycoproteins by isotope-tag method and capillary LC/ESI-MS.
abstract: OBJECTIVE
  To develop a rapid and sensitive method for monosaccharide composition analysis.
  
  
  METHODS
  Glycoprotein was first hydrolyzed to monosaccharides, which were subsequently reacetylated (amino monosaccharides), tagged with 2-aminopyridine and then separated and monitored in selected ion mode by CapGCC-LC/MS. The use of tetradeuterium labeled-aminopyridyl-monosaccharides prepared by tagging monosacahrides with hexadeuterium labeled 2-aminopyridine as internal standards improved the linearity and reproducibility in quantification.
  
  
  RESULTS
  This method was successfully applied to monosaccharide composition analysis of model glycoproteins, fetuin and erythropoietin down to 1 pmol monosaccharides.
  
  
  CONCLUSIONS
  This method has been shown to be highly sensitive and is applicable to monosaccharide composition analysis of glycoproteins.
authors.affiliation: Gene Engineering Department, Chengdu Institute of Biological Products, Chengdu 610063, China. yuanjinv@hotmail.com
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.forename: Nana
authors.initials: N
authors.lastname: Kawasaki
authors.forename: Noritaka
authors.initials: N
authors.lastname: Hashii
authors.forename: Satsuki
authors.initials: S
authors.lastname: Itoh
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004921:Erythropoietin / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006868:Hydrolysis; D007553:Isotope Labeling; D009005:Monosaccharides / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D000509:alpha-Fetoproteins / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D009005:Monosaccharides; D000509:alpha-Fetoproteins; D004921:Erythropoietin