title: Chemotypes significance of lichenized fungi by structural characterization of heteropolysaccharides from the genera Parmotrema and Rimelia.
abstract: Galactoglucomannans were isolated from the lichenized fungi of the genus Parmotrema (Parmotrema austrosinense, Parmotrema delicatulum, Parmotrema mantiqueirense, Parmotrema schindlerii, and Parmotrema tinctorum and that of Rimelia (Rimelia cetrata and Rimelia reticulata) via successive hot alkaline extraction and precipitation with Fehling solution. The structure of each polysaccharide was investigated using 13C NMR and HSQC-DEPT spectroscopy, methylation analysis, and HPSEC-MALLS. The galactoglucomannans had a (1-->6)-linked main chain of alpha-Manp units, substituted preferentially at O-2 and O-4 by alpha-Galp and beta-Galp nonreducing end-units, respectively. The C-1 region of the 13C NMR spectra of these heteropolysaccharides is typical of the lichen species, and is an additional tool in lichenized fungi classification.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, C.P. 19046, CEP 81531-990 Curitiba, PR, Brazil.
authors.forename: Elaine Rosechrer
authors.initials: ER
authors.lastname: Carbonero
authors.forename: Caroline Grassi
authors.initials: CG
authors.lastname: Mellinger
authors.forename: Sionara
authors.initials: S
authors.lastname: Eliasaro
authors.forename: Philip Albert James
authors.initials: PA
authors.lastname: Gorin
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000145:classification*; D002240:Carbohydrate Sequence; D008011:Lichens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry
chemical_list: D008351:Mannans; D011134:Polysaccharides; C443804:galactoglucomannan