[Experimental model for treating pulmonary metastatic melanoma using grape-seed extract, red wine and ethanol].
BACKGROUND
Melanoma is one of the neoplasias that most frequently metastasize, especially in the lung, where represents a challenge in oncology since current treatment is ineffective, and mortality is high.


METHODS
Swiss mice (n = 52) were inoculated with 0.5 x 106 B16F10 cell lines and, later, given an oral administration of grape-seed extract, red wine or ethanol. Metastatic nodules on the lung surface were counted and, after processing for microscopy, five sections were selected for image analysis and the invasion index was calculated.


RESULTS
Macroscopic analysis showed that grape-seed extract and red wine reduced the number of metastatic nodules by 26.07 and 20.81%, respectively, compared with a control group treated with ethanol. Microscopically, the reduction in the invasion index was 31.65 for grape-seed extract and 17.57% for red wine.


CONCLUSIONS
Ethanol administration significantly increased pulmonary metastasis while grape-seed extract and red wine led to their reduction.
Martínez Conesa
Cristina
C
Cátedra de Anatomía Patológica, Facultad de Medicina, Universidad de Murcia, Murcia, Spain. crismart@um.es
Vicente Ortega
Vicente
V
Yáñez Gascón
M Josefa
MJ
García Reverte
Juana Maria
JM
Canteras Jordana
Manuel
M
Alcaraz Baños
Miguel
M
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D000431:Ethanol / Q000627:therapeutic use*; D005260:Female; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D012639:Seeds; D027843:Vitis; D014920:Wine
D010936:Plant Extracts; D000431:Ethanol