Extracellular and intracellular acid-base status with regard to the energy metabolism in the oyster Crassostrea gigas during exposure to air.
The acid-base status of extra- and intracellular fluids was studied in relation to the anaerobic energy metabolism in the adductor muscle, mantle, gills, and heart of the marine bivalve Crassostrea gigas after exposure to air for periods of 2, 4, 8, 12, 24, and 48 h. Such exposure was found to cause a significant reduction in the pH in the hemolymph (pH(e)) within the first 4 h. The decrease in the pHe was accompanied by elevated Pco2 values, causing [HCO3-] to rise (respiratory acidosis). Thereafter, the pHe fell at a lower rate, and this fall was partially compensated for by a further increase in [HCO3-] in the hemolymph. The increase in the [Ca] levels in the hemolymph indicates a mobilization of Ca2+ from CaCO3 and the involvement of bicarbonates in the buffering of pHe. The main anaerobic end-products that accumulated in the tissues during the first stages of anaerobiosis were alanine and succinate, at a ratio of about 2 : 1. Later on, propionate and acetate were also accumulated at significant rates. In contrast to the adductor muscle, gills, and mantle, opine production in the heart was significant after 12-24 h of exposure to air. Determination of intracellular pH (pHi) revealed that there is a close relationship between the rate of anaerobic end-product accumulation and the extent of intracellular acidosis in the adductor muscle, mantle, and gills. On the contrary, accumulation of anaerobic end-products in the heart did not cause any significant change in its pHi. The intracellular nonbicarbonate, nonphosphate buffering value (beta (NB,NPi)) was determined to be higher in the heart than in the other three tissues and thus probably plays a crucial role in stabilizing heart pHi during exposure to air.
Michaelidis
Basile
B
Laboratory of Animal Physiology, Department of Zoology, Faculty of Science, School of Biology, University of Thessaloniki, GR-54124 Thessaloniki, Greece. michaeli@bio.auth.gr
Haas
Daniel
D
Grieshaber
Manfred K
MK
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000388:Air; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000097:blood; D002245:Carbon Dioxide / Q000032:analysis / Q000097:blood; D004734:Energy Metabolism / Q000502:physiology*; D045604:Extracellular Fluid / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010125:Ostreidae / Q000378:metabolism* / Q000502:physiology; D014781:Viscera / Q000378:metabolism
D002245:Carbon Dioxide; D002118:Calcium