Production of stilbenoids from the callus of Arachis hypogaea: a novel source of the anticancer compound piceatannol.
A new source to produce a significant quantity of a naturally occurring polyphenol, piceatannol, was investigated in this study. Both resveratrol and piceatannol are recognized as important ingredients in functional foods due to their beneficial health effects. However, unlike resveratrol, the piceatannol concentration in plants is very low. Thus, calluses of peanuts, an easily obtainable source, were chosen as the material to induce piceatannol production under controlled conditions. To induce resveratrol and piceatannol, calluses were exposed to the ultraviolet (UV) irradiation. Significant quantities of resveratrol and piceatannol were produced by calluses upon UV irradiation in both static and suspension culture conditions. The amounts of piceatannol and resveratrol produced in 1 g of calluses ranged from 2.17 to 5.31 microg and from 0.25 to 11.97 microg, respectively, in static culture. In suspension culture, the amounts of induced piceatannol and resveratrol were somewhat lower. The quantities of induced piceatannol and resveratrol reached a maximum at 18 h after UV irradiation treatment in static culture. In contrast, the levels of resveratrol and piceatannol remained almost constant throughout the experiments in suspension culture. The piceatannol produced by calluses in all studies was much higher than the values reported in the literature, whereas the resveratrol produced was comparable to reported values.
Ku
Kuo-Lung
KL
Department of Applied Chemistry and Graduate Institute of Agriculture, National Chiayi University, 300 University Road, Chiayi 60083, Taiwan. klku@mail.ncyu.edu.tw
Chang
Ping-Shun
PS
Cheng
Ya-Chin
YC
Lien
Ching-Yi
CY
D000970:Antineoplastic Agents; D010367:Arachis / Q000378:metabolism* / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D046509:Tissue Culture Techniques; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D013267:Stilbenes; C041525:3,3',4,5'-tetrahydroxystilbene; D000077185:Resveratrol