title: A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase.
abstract: Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.
authors.affiliation: State Research Institute of Genetic and Selection of Industrial Microorganisms, 1st Dorozhnii pr. 1, Moscow 117545, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Manukhov
authors.forename: Daria V
authors.initials: DV
authors.lastname: Mamaeva
authors.forename: Sergei M
authors.initials: SM
authors.lastname: Rastorguev
authors.forename: Nicolai G
authors.initials: NG
authors.lastname: Faleev
authors.forename: Elena A
authors.initials: EA
authors.lastname: Morozova
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Demidkina
authors.forename: Gennadii B
authors.initials: GB
authors.lastname: Zavilgelsky
mesh_terms: D001483:Base Sequence; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016971:Citrobacter freundii / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase