Calbindin D-28k is expressed in the microvascular basal lamina in the ventral horn at early time after transient spinal cord ischemia in the rabbit.
Much evidence has been accumulated that the increased expression of calbindin D-28k (CB) is involved in the blockade of calcium-evoked excitotoxicity in cerebral ischemia. We investigated the expression of CB in the basal lamina of microvessels in the ventral horn of the rabbit spinal cord after transient spinal cord ischemia. Spinal cord sections at the level of L7 were immunostained using monoclonal antibody raised against CB at light and electron microscopic levels. CB immunoreactivity was detected in the basal lamina of microvessels at 30 min after ischemic insult. By 3 h after ischemia, CB immunoreactivity was increased in the basal lamina of the microvessels. CB immunoreactivity began to decrease at 6 h after ischemia and nearly disappeared at 48 h after ischemic insult. For calcium detection in the blood vessels of spinal cord, we conducted an alizarin red staining. Alizarin red reactivity was detected in some microvessels at 3 h after ischemic insult. Our results suggest that the ectopic expression of CB in the microvascular basal laminae may be associated with the buffering of calcium in the endothelial cells of microvessels after ischemic damage.
Lee
Jae-Chul
JC
Department of Pharmacology, Ewha Medical School and Institute of Neuroscience, Ewha Womans University, Seoul 110-783, South Korea.
Hwang
In Koo
IK
Yoo
Ki-Yeon
KY
Jung
Ju-Young
JY
Cho
Jun Hwi
JH
Moon
Seung Myung
SM
Kang
Tae-Cheon
TC
Kim
Won-Ki
WK
Kim
Yong-Sun
YS
Won
Moo Ho
MH
D000818:Animals; D000880:Anthraquinones; D001485:Basement Membrane / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D002021:Buffers; D064026:Calbindins; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D008297:Male; D008833:Microcirculation / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011817:Rabbits; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D020760:Spinal Cord Ischemia / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D000880:Anthraquinones; D002021:Buffers; D064026:Calbindins; D007202:Indicators and Reagents; D064030:S100 Calcium Binding Protein G; C010078:alizarin; D002118:Calcium