title: ADAM-17 and TIMP3 protein and mRNA expression in spinal cord white matter of rats with acute experimental autoimmune encephalomyelitis.
abstract: Tumour necrosis factor (TNF) is a major immunomodulatory and proinflammatory cytokine implicated in the pathogenesis of multiple sclerosis (MS) and the animal model experimental autoimmune encephalomyelitis (EAE). ADAM-17 cleaves membrane-bound TNF into its soluble form. The distribution and level of ADAM-17 expression within spinal cords of Lewis rats with EAE was investigated. ADAM-17 was associated with endothelial cells in the naïve and pre-disease spinal cords. In peak disease astrocytic and inflammatory cells expressed ADAM-17. Upregulation of ADAM-17 mRNA expression was coupled with a decrease in mRNA levels of its inhibitor TIMP3 suggesting a role for ADAM-17 in EAE pathogenesis.
authors.affiliation: Biomedical Research Centre, Sheffield Hallam University, Howard St., Sheffield, S1 1WB, United Kingdom. j.plumb@shu.ac.uk
authors.forename: Jonnie
authors.initials: J
authors.lastname: Plumb
authors.forename: Alison K
authors.initials: AK
authors.lastname: Cross
authors.forename: Jessica
authors.initials: J
authors.lastname: Surr
authors.forename: Gail
authors.initials: G
authors.lastname: Haddock
authors.forename: Terence
authors.initials: T
authors.lastname: Smith
authors.forename: Rowena A D
authors.initials: RA
authors.lastname: Bunning
authors.forename: M Nicola
authors.initials: MN
authors.lastname: Woodroofe
mesh_terms: D051722:ADAM Proteins; D000072198:ADAM17 Protein; D000818:Animals; D000941:Antigens / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D053331:Ectodysplasins; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000378:metabolism*; D014841:von Willebrand Factor / Q000276:immunology
chemical_list: D000941:Antigens; D053331:Ectodysplasins; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D012333:RNA, Messenger; D019717:Tissue Inhibitor of Metalloproteinase-3; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D051722:ADAM Proteins; D008666:Metalloendopeptidases; D000072198:ADAM17 Protein; C000606464:Adam17 protein, rat