Taenia saginata: differential diagnosis of human taeniasis by polymerase chain reaction-restriction fragment length polymorphism assay.
Speciation of Taenia in human stool is important because of their different clinical and epidemiological features. DNA analysis has recently become possible which overcomes the problems of differentiating human taeniid cestodes morphologically. In the present study, we evaluated PCR coupled to restriction fragment length polymorphism to differentiate Taenia solium from Taenia saginata eggs present in fecal samples from naturally infected patients. A different DraI-RFLP pattern: a two-band pattern (421 and 100 bp) for T. saginata and a three-band pattern (234, 188, and 99 bp) for T. solium was observed allowing the two species to be separated. The lower detection limit of the PCR-RFLP using a non-infected fecal sample prepared with a given number of T. saginata eggs was 34 eggs in 2 g stool sediment. The 521 bp mtDNA fragment was detected in 8 out of 12 Taenia sp. carriers (66.6%). Of these, three showed a T. solium pattern and five a T. saginata pattern.
Nunes
Cáris Maroni
CM
UNESP, Campus Araçatuba, Department of Animal Health and Production, SP, Brazil. caris@fmva.unesp.br
Dias
Ana Karina Kerche
AK
Dias
Francisca Elda Ferreira
FE
Aoki
Sérgio Moraes
SM
de Paula
Henrique Borges
HB
Lima
Luis Gustavo Ferraz
LG
Garcia
José Fernando
JF
D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000469:parasitology*; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D016622:Silver Staining; D013552:Swine; D041202:Taenia saginata / Q000235:genetics / Q000302:isolation & purification*; D041201:Taenia solium / Q000235:genetics / Q000302:isolation & purification; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases