title: Melatonin protects against neuronal damage induced by 3-nitropropionic acid in rat striatum.
abstract: In this study, the protective effects of melatonin were evaluated against 3-nitropropionic acid (3-NP)-induced striatal neuronal damage in rats. Lesions were induced in the right striatum of Sprague-Dawley rats by stereotaxic injection with 3-NP and melatonin was intraperitoneally administered both 30 min before and 60 min after 3-NP injection. And rats continuously received melatonin daily for 3 days. As indicators of oxidative damage, lipid peroxidation and protein oxidation in the lesioned striatum were measured at 1 day after 3-NP injection. Levels of malondialdehyde (MDA) and protein carbonyl were significantly increased by 3-NP injection, but reduced in the melatonin-treated rats. Four days post-lesion, large lesions and extensive neuronal damage were produced in the 3-NP-injected striata, as revealed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. In addition, marked ipsilateral rotational behavior following apomorphine challenge and a decrease of dopamine content in the lesioned striatum were observed in the 3-NP-injected rats. However, melatonin treatment significantly attenuated the 3-NP-induced neuronal damage, reduced the degree of asymmetric rotational behavior, and restored the dopamine level in the lesioned striatum. The present results indicate that melatonin effectively protects against the neuronal damage caused by 3-NP in vivo and that the neuroprotective effects of melatonin may be related to antioxidant action.
authors.affiliation: Department of Pharmacology, College of Medicine and Neuroscience Research Institute of Medical Research Center, Seoul National University, Korea.
authors.forename: Eunjoo
authors.initials: E
authors.lastname: Nam
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Seong Eun
authors.initials: SE
authors.lastname: Koh
authors.forename: Wan Seok
authors.initials: WS
authors.lastname: Joo
authors.forename: Sungho
authors.initials: S
authors.lastname: Maeng
authors.forename: Heh In
authors.initials: HI
authors.lastname: Im
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D003342:Corpus Striatum / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D009048:Motor Skills; D009410:Nerve Degeneration / Q000139:chemically induced; D009474:Neurons / Q000187:drug effects* / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins; D009574:Nitro Compounds; D018384:Oxidative Stress / Q000187:drug effects; D011422:Propionates; D051381:Rats; D017207:Rats, Sprague-Dawley; D045442:Rotarod Performance Test
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D009498:Neurotoxins; D009574:Nitro Compounds; D011422:Propionates; D008550:Melatonin; C015392:3-nitropropionic acid