The intracellular fate of Salmonella depends on the recruitment of kinesin.
Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. The success of this pathogen depends on its capacity to proliferate within host cells in a membrane-bound compartment. We found that the Salmonella-containing vacuole recruited the plus-end-directed motor kinesin. Bacterial effector proteins translocated into the host cell by a type III secretion system antagonistically regulated this event. Among these effectors, SifA targeted SKIP, a host protein that down-regulated the recruitment of kinesin on the bacterial vacuole and, in turn, controlled vacuolar membrane dynamics.
Boucrot
Emmanuel
E
Centre d'Immunologie de Marseille-Luminy, CNRS-INSERM-Université de la Méditerranée, Parc Scientifique de Luminy, Case 906-13288 Marseille Cedex 9, France.
Henry
Thomas
T
Borg
Jean-Paul
JP
Gorvel
Jean-Pierre
JP
Méresse
Stéphane
S
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D020816:Amino Acid Motifs; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D007425:Intracellular Membranes / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism*; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D014617:Vacuoles / Q000378:metabolism / Q000382:microbiology*; D014774:Virulence
D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; C466999:SPHKAP protein, human; C421862:SifA protein, Salmonella; D004398:Dyneins; D016547:Kinesins