title: Kanamycin A-derived cationic lipids as vectors for gene transfection.
abstract: Cationic lipids nowadays constitute a promising alternative to recombinant viruses for gene transfer. We have recently explored the transfection potential of a new class of lipids based upon the use of aminoglycosides as cationic polar headgroups. The encouraging results obtained with a first cholesterol derivative of kanamycin A prompted us to investigate this family of vectors further, by modulating the constituent structural units of the cationic lipid. For this study, we have investigated the transfection properties of a series of new derivatives based on a kanamycin A scaffold. The results primarily confirm that aminoglycoside-based lipids are efficient vectors for gene transfection both in vitro and in vivo (mouse airways). Furthermore, a combination of transfection and physicochemical data revealed that some modifications of the constitutive subunits of kanamycin A-based vectors were associated with substantial changes in their transfection properties.
authors.affiliation: Laboratoire de Chimie des Interactions Moléculaires, Collège de France, CNRS UPR 285, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France. matthieu.sainlos@college-de-france.fr
authors.forename: Matthieu
authors.initials: M
authors.lastname: Sainlos
authors.forename: Michelle
authors.initials: M
authors.lastname: Hauchecorne
authors.forename: Noufissa
authors.initials: N
authors.lastname: Oudrhiri
authors.forename: Samia
authors.initials: S
authors.lastname: Zertal-Zidani
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Aissaoui
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Vigneron
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lehn
authors.forename: Pierre
authors.initials: P
authors.lastname: Lehn
mesh_terms: D000617:Aminoglycosides / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002412:Cations; D005822:Genetic Vectors; D007612:Kanamycin / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D012137:Respiratory System / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002412:Cations; D008055:Lipids; D007612:Kanamycin