Protein-functionalized polymer brushes.
A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.
Tugulu
Stefano
S
Laboratoire des Polymères, Institut des Matériaux, Bâtiment MX-D, and Laboratoire d'Ingénierie des Protéines, Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
Arnold
Anke
A
Sielaff
India
I
Johnsson
Kai
K
Klok
Harm-Anton
HA
D011108:Polymers / Q000138:chemical synthesis*; D011485:Protein Binding; D011506:Proteins / Q000138:chemical synthesis*
D011108:Polymers; D011506:Proteins