Immunohistochemical co-expression of carbonic anhydrase II with Kv1.4 and TRPV1 in rat small-diameter trigeminal ganglion neurons.
The co-expression of carbonic anhydrase II (CAII) with the voltage-gated potassium channel subtype 1.4 (Kv1.4) or the vanilloid receptor (TRPV1) was examined in adult rat trigeminal ganglion (TG) neurons by using the immunofluorescence method. The small-diameter Kv.1.4-positive TG neurons co-expressed CAII immunoreactivity (47%). Most TRPV1-positive TG neurons (79%) had the CAII immunoreactivity, but showed a lack of immunoreactivity for a neurofilament protein (NF200), a maker of large TG neurons with myelinated axons. The fact that CAII-immunoreactive TG neurons revealed a common expression of both Kv1.4 and TRPV1 leads us to suggest that CAII may be one of the nociceptive neuronal markers.
Tanimoto
Takeshi
T
Department of Physiology, Nippon Dental University, School of Dentistry at Tokyo, 1-9-20 Fujimi, Tokyo 102-8159, Japan. ttt1224@tokyo.ndu.ac.jp
Takeda
Mamoru
M
Nasu
Masanori
M
Kadoi
Jun
J
Matsumoto
Shigeji
S
D000818:Animals; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D048429:Cell Size; D007150:Immunohistochemistry / Q000379:methods; D007473:Ion Channels / Q000378:metabolism*; D051665:Kv1.4 Potassium Channel; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D050916:TRPV Cation Channels; D012668:Trigeminal Ganglion / Q000166:cytology*
D007473:Ion Channels; C496979:Kcna4 protein, rat; D051665:Kv1.4 Potassium Channel; D016900:Neurofilament Proteins; D024642:Potassium Channels, Voltage-Gated; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C051774:neurofilament protein H; D024402:Carbonic Anhydrase II