Laboratory study of treatment of trichloroethene by chemical oxidation followed by bioremediation.
Studies were conducted with columns containing soil and emplaced trichloroethene (TCE) to investigate the potential for TCE source zone remediation with chemical oxidation followed by biologically mediated reductive dehalogenation. Following permanganate flushing of four columns, which resulted in rapid but incomplete removal of TCE DNAPL, no biological activity was observed following the addition of distilled water amended with ethanol and acetate, including two of the four columns that were bioaugmented with a TCE-dechlorinating microbial culture. Flushing with unsterilized site groundwater led to consumption of acetate and ethanol, accompanied by manganese reduction and methanogenesis. Reductive dechlorination of TCE to cis-1,2-dichloroethene (cis-DCE) followed the onset of ethanol and acetate biodegradation in bioaugmented columns only. Partial dechlorination of TCEto ethene was observed only in one of the bioaugmented columns after it was inoculated for a third time. At the end of the study (290 days), a trace amount of cis-DCE was observed in one of the two columns which was not bioaugmented. Reduced conditions created by biostimulation were also conducive to reduction of Mn(IV) from MnO2 in both bioaugmented and nonbioaugmented columns resulting in an increased dissolved manganese (Mn2+) concentration in groundwater.
Hrapovic
Leila
L
Department of Civil Engineering, University of Toronto, Toronto, Canada.
Sleep
Brent E
BE
Major
David J
DJ
Hood
Eric D
ED
D000085:Acetates / Q000737:chemistry; D001673:Biodegradation, Environmental; D002713:Chlorine / Q000737:chemistry; D004000:Dichloroethylenes / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D008345:Manganese / Q000032:analysis / Q000737:chemistry; D017895:Manganese Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014241:Trichloroethylene / Q000737:chemistry / Q000378:metabolism*; D014873:Water Pollutants / Q000032:analysis / Q000378:metabolism*
D000085:Acetates; D004000:Dichloroethylenes; D017895:Manganese Compounds; D010087:Oxides; D012989:Soil Pollutants; D014873:Water Pollutants; C048856:permanganic acid; D014241:Trichloroethylene; D000431:Ethanol; D008345:Manganese; D002713:Chlorine; C035384:1,2-dichloroethylene