title: Low prevalence of antibodies against heat shock protein 10 of Chlamydophila pneumoniae in patients with coronary heart disease.
abstract: In this study the prevalence of antibodies against the heat shock protein 10 (HSP10) of Chamydophila pneumoniae (CP) (as assessed by ELISA) in patients with coronary heart disease (CHD) and seropositive or seronegative to CP, as assessed by microimmunofluorescence (MIF), was investigated. The controls were age- and sex-matched healthy subjects. The HSP10 preparation used throughout this study was a 6-his-tagged recombinant protein preliminarily shown to be immunogenic in mice. Low level IgG reactivity against CP-HSP10 was detected in 19 out of 200 and 5 out of 100 CHD patients and controls, respectively. No IgM or IgA isotypes were found. Furthermore, there was no difference in the frequency or level of anti-HSP10 IgG between CP-positive and CP-negative sera either in patients (11/140=7.9% vs. 8/60=13%) or in healthy subjects (3/40=7.5% vs. 2/60=3.3%). Overall, our data indicate that CP-HSP10, at variance with CP-HSP60, to which it is genetically and physiologically linked, should not be regarded as a major expressed immunogen or a marker of infection by CP in CHD patients.
authors.affiliation: Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ciervo
authors.forename: Andrea
authors.initials: A
authors.lastname: Petrucca
authors.forename: Umbertina
authors.initials: U
authors.lastname: Villano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Fioroni
authors.forename: Antonio
authors.initials: A
authors.lastname: Cassone
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D016022:Case-Control Studies; D018835:Chaperonin 10 / Q000235:genetics / Q000276:immunology*; D023521:Chlamydophila Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D003327:Coronary Disease / Q000209:etiology / Q000276:immunology* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018835:Chaperonin 10; D004269:DNA, Bacterial; D011994:Recombinant Proteins