title: [Development and certification of libraries of the MDCK continuous cell line for production of influenza vaccine].
abstract: Seeding and working banks of the continuous MDCK cell culture suitable for the production of cultured influenza vaccine were created and deposited at liquid nitrogen temperature at the "Vector" State Research Center of Virology and Biotechnology. The MDCK cell culture was shown to have morphology typical of the discussed cell line; it does not have any alien agents and is oncogenically safe; its enzimogram and karyotype are typical of the donor line; finally, its biological properties are stable during a long period of cultivation and its sensitivity to influenza virus is high, therefore, it can be recommended for the production of influenza vaccine. The continuous MDCK cell line was certified at Tarasevich Committee and was recommended by the MIBP Committee, Russia's Health Ministry, for its use as a substrate in the production of diagnostic and preventive immunoglobulins.
authors.forename: I F
authors.initials: IF
authors.lastname: Radaeva
authors.forename: T D
authors.initials: TD
authors.lastname: Kolokol'tsova
authors.forename: E A
authors.initials: EA
authors.lastname: Nechaeva
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: T V
authors.initials: TV
authors.lastname: Il'ina
authors.forename: T N
authors.initials: TN
authors.lastname: Getmanova
authors.forename: L S
authors.initials: LS
authors.lastname: Baltueva
authors.forename: A S
authors.initials: AS
authors.lastname: Grafodatskiĭ
authors.forename: Iu Z
authors.initials: IuZ
authors.lastname: Gendon
authors.forename: E M
authors.initials: EM
authors.lastname: Petruchuk
mesh_terms: D000818:Animals; D002460:Cell Line / Q000382:microbiology / Q000648:ultrastructure / Q000821:virology; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D007252:Influenza Vaccines / Q000096:biosynthesis / Q000592:standards*; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification; D014776:Virus Cultivation / Q000592:standards
chemical_list: D004269:DNA, Bacterial; D007252:Influenza Vaccines; D012367:RNA, Viral