24p3 in differentiation of myeloid cells.
24p3 is a secreted lipocalin that has been variously related to apoptosis, proliferation, and the neutrophil lineage of blood cells. We have investigated the expression of 24p3 mRNA and protein in myeloid cell lines induced to differentiate by insulin-like growth factor 1 (IGF-1) and the granulocytic-colony simulating factor (G-CSF). Both these growth factors, which cause myeloid cells to differentiate into granulocytes, induced a marked increase in the expression of both 24p3 protein and mRNA. The mRNA especially appeared early after the cells were induced with either IGF-1 or G-CSF, at a time when the cells were still proliferating and are morphologically undifferentiated. 24p3 can be considered an early marker of granulocytic differentiation.
Liu
Mingli
M
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Prisco
Marco
M
Drakas
Robert
R
Searles
David
D
Baserga
Renato
R
D000209:Acute-Phase Proteins / Q000378:metabolism*; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D000071068:Lipocalin-2; D054834:Lipocalins; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D000209:Acute-Phase Proteins; D015415:Biomarkers; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D012333:RNA, Messenger; C071332:Lcn2 protein, mouse; D016179:Granulocyte Colony-Stimulating Factor; D007334:Insulin-Like Growth Factor I