title: Metabolic activation-related CD147-CD98 complex.
abstract: Cell surface CD147 protein promotes production of matrix metalloproteinases and hyaluronan, associates with monocarboxylate transporters and integrins, and is involved in reproductive, neural, inflammatory, and tumor functions. Here we combined covalent cross-linking, mass spectrometric protein identification, and co-immunoprecipitation to show selective CD147 association with three major types of transporters (CD98 heavy chain (CD98hc)-L-type amino acid transporter, ASCT2, and monocarboxylate transporters) as well as a regulator of cell proliferation (epithelial cell adhesion molecule). In the assembly of these multicomponent complexes, CD147 and CD98hc play a central organizing role. RNA interference knock-down experiments established a strong connection between CD147 and CD98hc expression and a strong positive association of CD147 (and CD98hc) with cell proliferation. As the CD147-CD98hc complex and proliferation diminished, AMP-activated protein kinase (a cellular "fuel gauge") became activated, indicating a disturbance of cellular energy metabolism. Our data point to a CD147-CD98 cell surface supercomplex that plays a critical role in energy metabolism, likely by coordinating transport of lactate and amino acids. Furthermore we showed how covalent cross-linking, together with mass spectrometry, can be used to identify closely associated transmembrane proteins. This approach should also be applicable to many other types of transmembrane proteins besides those associated with CD98hc and CD147.
authors.affiliation: Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Daosong
authors.initials: D
authors.lastname: Xu
authors.forename: Martin E
authors.initials: ME
authors.lastname: Hemler
mesh_terms: D055372:AMP-Activated Protein Kinases; D015703:Antigens, CD / Q000378:metabolism*; D051926:Basigin; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003432:Cross-Linking Reagents; D027282:Fusion Regulatory Protein 1, Heavy Chain / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D007668:Kidney / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015703:Antigens, CD; C497886:BSG protein, human; D003432:Cross-Linking Reagents; D027282:Fusion Regulatory Protein 1, Heavy Chain; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D051926:Basigin; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases