[Interleukin-8 protein and gene expression in atherosclerotic lesions of hyperlipemia rabbits].
Interleukin-8 is CXC chemokine that is initially discovered using chemotaxis and the activation of neutrophils and induces the migration and proliferation of smooth muscle cells. Interleukin-8 is a potent angiogenic factor that may play a role in atherosclerosis. To establish the temporal correlation between IL-8 expression and plaque development, we examined the expression during atherosclerosis of hyperlipemia rabbits using immunohistochemistry, ELISA, in situ hybridization. By location of immunohistochemistry, the expression of IL-8 protein increased obviously in intima of hyperlipemia rabbits at 8 and 12 week. Quantitative analysis of the expression of IL-8 Immunohistochemistry indicated that positive area of AS model was 4.48 times and 8.76 times that of control group at 8 and 12 week. The valuation of IOD of AS model was 4.16 times and 4.36 times that of control group at 8 and 12 week. By specific ELISA, the ratio of the IL-8 protein to total protein of AS model was 1.84 times and 2.06 times that of control group at 8 and 12 week. By location of in situ hybridization, positive location was strong in intima of hyperlipemia rabbits at 8 week. We observed the dynamic alteration of interleukin-8 protein and gene expression in atherosclerotic lesions of hyperlipemia rabbits with establishing model. Interleukin-8 protein and gene expression was up-regulation in the development of fatty streaks in hyperlipemia rabbit.
Nie
Yongmei
Y
Department of Biomedical Engineering, West China Medical Center of Sichuan University, Chengdu 610041, China.
Chen
Huaiqing
H
Cheng
Min
M
Liu
Xiaojing
X
Deng
Yiling
Y
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism*; D006949:Hyperlipidemias / Q000150:complications*; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits
D016209:Interleukin-8; D012333:RNA, Messenger