title: Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system.
abstract: Cotesia plutellae polydnaviruses (CpBV) has a segmented genome consisting of multiple circular double stranded DNAs. Recently, we have developed an easy, simple, and convenient system based on Tn7 transposition in order to clone genomic segments of CpBV in Escherichia coli cell and designated plasmid capture system (PCS). The PCS donor-S transferred a pUC19 origin of replication and an ampicillin resistance marker into CpBV genomic DNA by in vitro transposition. Through PCS system, we were able to clone 53 genomic clones ranging from 0.1 to 25.5 kb and further they were classified into 29 segments by their sizes and restriction endonuclease patterns. Among them, a complete nucleotide sequence of CpBV-S28 segment was determined and 10 putative genes were predicted from this segment. Interestingly, 9 of 10 putative ORFs had high level of similarities with catalytic domain of protein tyrosine phosphatase. Also, ORF2807 showed similarity with EP1-like proteins of C. congregata polydnavirus.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Joong Nam
authors.initials: JN
authors.lastname: Kang
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Shim
authors.forename: Soo Dong
authors.initials: SD
authors.lastname: Woo
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Ming Shun
authors.initials: MS
authors.lastname: Li
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016679:Genome, Viral; D010957:Plasmids / Q000235:genetics*; D018071:Polydnaviridae / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements