title: Detection of T lymphocyte subsets of children with Helicobacter pylori infection.
abstract: OBJECTIVE
  To study the transformation of T lymphocyte subsets in children with Heliobacter pylori (H pylori) infection.
  
  
  METHODS
  The H pylori infection status were determined by a combination of ELISA and Western blot (immunoblot) technique in 98 children and T lymphocyte subsets in peripheral blood were determined by flow cytometrical analysis.
  
  
  RESULTS
  There were 75 children positive with H pylori infection and 23 negative in 98 children. Comparing the proportion of peripheral blood T lymphocytic subsets in children with H pylori infection and without H pylori infection, it was found that a higher proportion of CD4 T-cells in infected children (39.02+/-7.71 vs 34.25+/-10.73, t = 2.246,P<0.05) and higher value of CD4 to CD8 T-cells ratio (1.51+/-0.52 vs 1.25, t = 2.104, P<0.05) were present, but there were not significant differences in CD3 T-cells and CD8 T-cells (73.11+/-10.02 vs 69.49+/-17.08, 27.22+/-6.07 vs 28.27+/-8.67, P>0.05).
  
  
  CONCLUSIONS
  Th1 cell-mediated immune responses may be induced by H pylori infection in children.
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. wll276@yahoo.com.cn
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wang
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Shan
authors.forename: Hai-Xing
authors.initials: HX
authors.lastname: Jiang
authors.forename: Dinh-Binh
authors.initials: DB
authors.lastname: Tran
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Xiang-Zhi
authors.initials: XZ
authors.lastname: Xie
authors.forename: Dan-Mei
authors.initials: DM
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D002648:Child; D005260:Female; D016481:Helicobacter Infections / Q000097:blood*; D016480:Helicobacter pylori; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*