Innervation of stem cell-derived neurons into auditory epithelia of mice.
This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice for 7 days. Histological analysis demonstrated massive elongation of neurites from embryonic stem cell-derived neurons toward auditory hair cells. Embryonic stem cell-derived neurites were adjacent to or surrounding hair cells, and exhibited expression of synaptophysin, a marker for synaptic vesicles. These findings demonstrate the ability of embryonic stem cell-derived neurons for reinnervation into auditory epithelia, indicating a high potential of embryonic stem cell-derived neurons as transplants for replacing spiral ganglion neurons.
Matsumoto
Masahiro
M
Department of Otolaryngology, Head and Neck Surgery, Kyoto University, Graduate School of Medicine, Kyoto, Japan.
Nakagawa
Takayuki
T
Higashi
Toru
T
Kim
Tae-Soo
TS
Kojima
Ken
K
Kita
Tomoko
T
Sakamoto
Tatsunori
T
Ito
Juichi
J
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004398:Dyneins / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008813:Mice, Inbred ICR; D000081412:Myosin VIIa; D009218:Myosins / Q000378:metabolism; D016501:Neurites / Q000502:physiology; D046569:Neuroepithelial Cells / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D013136:Spiral Ganglion / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*; D016708:Synaptophysin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
C000631456:Myo7a protein, mouse; D000081412:Myosin VIIa; D016708:Synaptophysin; D014404:Tubulin; D049452:Green Fluorescent Proteins; D009218:Myosins; D004398:Dyneins