A chitosan-arginine conjugate as a novel anticoagulation biomaterial.
Chitosan (CS) was modified with arginine using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as coupling agents. FTIR and 13C NMR spectra showed that arginine was chemically coupled to CS to form a chitosan-arginine conjugate (CS-ArgC). The substitution degree of arginine in CS estimated from elemental analysis was 20.1%. The circular dichroism spectra indicated that the incorporation of arginine significantly altered the conformation of thrombin; while no obvious variation in the conformation of thrombin was observed with the addition of CS. The anticoagulation activity of glucose aldehyde crosslinked CS-ArgC and CS membranes was evaluated by assaying prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT). The APTT of CS-ArgC membrane was prolonged two times as that of CS counterpart, suggesting that the CS-ArgC is a promising candidate as an anticoagulation biomaterial.
Liu
W G
WG
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China. wgliu@tju.edu.cn
Zhang
J R
JR
Cao
Z Q
ZQ
Xu
F Y
FY
Yao
K D
KD
D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D001120:Arginine / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D005240:Feasibility Studies; D006801:Humans; D008422:Materials Testing
D000925:Anticoagulants; D001672:Biocompatible Materials; D048271:Chitosan; D001120:Arginine