Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria.
Strain Kw07T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07T belongs to the alpha-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033T(97.8%). Chemotaxonomic data revealed that strain Kw07T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C18:1 omega7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07T represents a distinct species. Based on these data, Kw07T (=KCTC 12209T=NBRC 100800T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.
Kim
Myung Kyum
MK
Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Im
Wan-Taek
WT
Ohta
Hiroyuki
H
Lee
Myungjin
M
Lee
Sung-Taik
ST
D001482:Base Composition; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D042301:Sphingomonadaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001617:beta-Glucosidase / Q000096:biosynthesis*
D001617:beta-Glucosidase