title: Leuconostoc durionis sp. nov., a heterofermenter with no detectable gas production from glucose.
abstract: Three lactic acid bacterial (LAB) strains obtained from a Malaysian acid-fermented condiment, tempoyak (made from pulp of the durian fruit), showed analogous but distinct patterns after screening by SDS-PAGE of whole-cell proteins and comparison with profiles of all recognized LAB species. 16S rRNA gene sequencing of one representative strain showed that the taxon belongs phylogenetically to the genus Leuconostoc, with its nearest neighbour being Leuconostoc fructosum (98 % sequence similarity). Biochemical characteristics and DNA-DNA hybridization experiments demonstrated that the strains differ from Leuconostoc fructosum and represent a single, novel Leuconostoc species for which the name Leuconostoc durionis sp. nov. is proposed. The type strain is LMG 22556(T) (= LAB 1679(T) = D-24(T) = CCUG 49949(T)).
authors.affiliation: Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
authors.forename: J J
authors.initials: JJ
authors.lastname: Leisner
authors.affiliation: BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing (IMDO), Department of Applied Biological Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Van der Meulen
authors.affiliation: BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Lefebvre
authors.affiliation: BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Engelbeen
authors.affiliation: BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Hoste
authors.affiliation: Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
authors.forename: B G
authors.initials: BG
authors.lastname: Laursen
authors.affiliation: Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Grønnegårdsvej 15, 1870 Frederiksberg C., Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Bay
authors.affiliation: Department of Food Science, Universiti Putra Malaysia, Serdang, Selangor D.E., Malaysia.
authors.forename: G
authors.initials: G
authors.lastname: Rusul
authors.affiliation: Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing (IMDO), Department of Applied Biological Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: De Vuyst
authors.affiliation: BCCM LMG Bacteria Collection, Laboratory of Microbiology, Gent University, Gent, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Swings
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D003212:Condiments / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005947:Glucose / Q000378:metabolism*; D007934:Leuconostoc / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005947:Glucose