Detection of lipopolysaccharide and lipid a employing a spermine-pyrene conjugate.
An amphiphilic fluorescent spermine-pyrene conjugate (Sp-Py) is shown to interact with lipopolysaccharide (LPS) or lipid A in aqueous solution. This complexation was studied by UV/visible absorption and steady-state fluorescence spectroscopy. In the presence of LPS or lipid A, Sp-Py displayed self-aggregation, resulting in the appearance of chromophore dimer absorption and fluorescence at the expense of probe monomer features. The equivalent weight of LPS or lipid A and the binding constants for Sp-Py complexation were estimated. The change of the ratio of monomer to dimer emission with concentration of LPS or lipid A indicated that Sp-Py is a sensitive fluorescence probe for the endotoxins in aqueous media.
Jones
Guilford
G
Department of Chemistry and Photonics Center, Boston University, Boston, Massachusetts 02215, USA. giljones@chem.bu.edu
Jiang
Hui
H
D008050:Lipid A / Q000032:analysis* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013096:Spermine / Q000737:chemistry*; D017186:Titrimetry
D008050:Lipid A; D008070:Lipopolysaccharides; D011721:Pyrenes; D013096:Spermine; C030984:pyrene