Collagen XVII and BPAG1 expression in the retina: evidence for an anchoring complex in the central nervous system.
The ectoderm gives rise not only to the skin but also to the entire CNS. This common embryonic lineage suggests that some molecular isoforms might serve analogous functions in both tissues. Indeed, not only are laminins important components of dermal adhesion mechanisms, but they also regulate some aspects of synaptic development in both the CNS and the PNS. In the skin, laminins are part of a hemidesmosome complex essential for basal keratinocyte adhesion that includes collagen XVII (BP180) and BPAG1 (dystonin/BP230). Here, we show that CNS neurons also express collagen XVII and BPAG1 and that these molecules are expressed in the adult and developing retina. In the retina, isoforms of collagen XVII and BPAG1 are colocalized with laminins at photoreceptor synapses and around photoreceptor outer segments; both molecules are expressed by rods, whereas cones express collagen XVII but not BPAG1. Moreover, biochemical data demonstrate that collagen XVII complexes with retinal laminins. We propose that collagen XVII and BPAG1 isoforms may help to anchor elements of the rod photoreceptor cytomatrix to the extracellular matrix.
Claudepierre
Thomas
T
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, and the Tufts Center for Vision Research, Boston, Massachusetts 02111, USA.
Manglapus
Mary K
MK
Marengi
Nathan
N
Radner
Stephanie
S
Champliaud
Marie-France
MF
Tasanen
Kaisa
K
Bruckner-Tuderman
Leena
L
Hunter
Dale D
DD
Brunken
William J
WJ
D000818:Animals; D000831:Animals, Newborn; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism*; D017364:Collagenases / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D016547:Kinesins; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D009124:Muscle Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D024041:Non-Fibrillar Collagens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism; D011257:Pregnancy Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism; D000097805:Collagen Type XVII
D016282:Aspartic Acid Endopeptidases; D001324:Autoantigens; D002135:Calcium-Binding Proteins; D017364:Collagenases; D016547:Kinesins; D007797:Laminin; D008562:Membrane Glycoproteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D019887:Peanut Agglutinin; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D014746:Vimentin; D000097805:Collagen Type XVII; C033652:postsynaptic density proteins; C058783:laminin beta2; C074152:kinesin-II; C094766:pregnancy-associated glycoprotein 1