Pre-column derivatization of rofecoxib for determination in serum by HPLC.
An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).
Amini
M
M
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Hamedani
M Pirali
MP
Vosooghi
M
M
Nabavi
M
M
Shafiee
A
A
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003500:Cyclization; D052246:Cyclooxygenase 2 Inhibitors / Q000097:blood* / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D007783:Lactones / Q000097:blood* / Q000737:chemistry / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010616:Phenanthrenes / Q000097:blood* / Q000737:chemistry / Q000528:radiation effects; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000097:blood* / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
D052246:Cyclooxygenase 2 Inhibitors; D007783:Lactones; D010616:Phenanthrenes; D013450:Sulfones; C116926:rofecoxib