Development of a rapid autopsy program for studies of brain immunity.
Human glia are essential cellular models used for studies of neurodegenerative diseases. Fetal neuroglia are commonly used, as they can be recovered in large quantities and sustained for long periods in culture. However, fetal neuroglia may have limitations in reflecting adult diseases and additionally can pose ethical issues in translating products of abortion for research use. To address these concerns, we developed a rapid autopsy program to procure age- and disease-specific neuroglia from adult brain tissues within hours of death. The challenges in developing this initiative, reflecting experiences from 69 autopsies over 4 years, are presented.
Ghorpade
Anuja
A
Laboratory of Cellular Neuroimmunology, 985215 Nebraska Medical Center, Omaha, NE, 68198-5215, USA. aghorpad@unmc.edu
Bruch
Leslie
L
Persidsky
Yuri
Y
Chin
Betty
B
Brown
William H C
WH
Borgmann
Kathleen
K
Persidsky
Raisa
R
Wu
Li
L
Holter
Spring
S
Cotter
Robin
R
Faraci
Jill
J
Heilman
David
D
Meyer
VaKara
V
Potter
Jane F
JF
Swindells
Susan
S
Gendelman
Howard E
HE
D000046:Academic Medical Centers / Q000379:methods; D000328:Adult; D001344:Autopsy / Q000379:methods*; D001921:Brain / Q000276:immunology* / Q000473:pathology*; D002478:Cells, Cultured; D003159:Community-Institutional Relations; D006801:Humans; D007111:Immunity, Cellular; D009457:Neuroglia / Q000276:immunology / Q000473:pathology; D016730:Program Development / Q000379:methods*; D013997:Time Factors; D014015:Tissue Banks / Q000458:organization & administration; D009927:Tissue and Organ Procurement