Isolation and functional characterization of siderophore-producing lead- and cadmium-resistant Pseudomonas putida KNP9.
Heavy metals, being phytotoxic, cause growth inhibition and even plant death. Siderophore-producing bacterial strain KNP9 is growth promoting and has been isolated from Panki Power Plant, Kanpur, India. It simulated significant (p > 5%) root and shoot growth of mung bean to the extent of 16.48% and 28.80%, respectively in the presence of CdCl(2) (110 microM: ). However, the increase in root and shoot growth was 20% and 19.5%, respectively, in the presence of (CH(3)COO)(2)Pb (660 microM: ). Moreover, concentration of accumulated lead and cadmium in root and shoot was also reduced in the presence of this isolate ranging from 37.5 to 93.19%. A moderate reduction in chlorophyll content (39.14%) in the presence of 110 microM: CdCl(2) was rescued by bioinoculant KNP9. However, the 19.58% decrease in chlorophyll content in the case of lead acetate remained unchanged even in the presence of KNP9. Nevertheless, 16S ribosomal DNA (rDNA) sequencing identified KNP9 as a strain of Pseudomonas putida.
Tripathi
Manishi
M
Department of Microbiology, CBSH, G.B. Pant University of Agriculture & Technology, Pantnagar-263145, India.
Munot
Hitendra P
HP
Shouche
Yogesh
Y
Meyer
Jean Marie
JM
Goel
Reeta
R
D002104:Cadmium / Q000032:analysis; D019256:Cadmium Chloride; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics; D007854:Lead / Q000032:analysis; D009942:Organometallic Compounds; D027805:Phaseolus / Q000737:chemistry / Q000254:growth & development* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D018520:Plant Shoots / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D011210:Power Plants; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants
D004269:DNA, Bacterial; D009942:Organometallic Compounds; D010937:Plant Growth Regulators; D017262:Siderophores; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D019256:Cadmium Chloride; C008261:lead acetate