Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers.
The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 F2 individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5' upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.
Lee
Choong-Jae
CJ
Department of Plant Science, College of Agriculture and Life Sciences, and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-921, Korea.
Yoo
Eunyoung
E
Shin
Juhyun
J
Shin
Joo Hyun
JH
Lee
Jemin
J
Hwang
Hee-Sook
HS
Kim
Byung-Dong
BD
D001947:Breeding; D002211:Capsaicin / Q000737:chemistry*; D002212:Capsicum / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation; D032461:Chromosomes, Plant; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D009812:Odorants; D036226:Seedlings; D017384:Sequence Deletion
D005819:Genetic Markers; D002211:Capsaicin