title: An AFLP-markers based genetic linkage map of Heterobasidion annosum locating intersterility genes.
abstract: A genetic linkage map of the basidiomycete Heterobasidion annosum, casual agent of root rot in conifers, was constructed from a compatible mating between isolates from the North American S and P intersterility groups. In a population consisting of 102 progeny isolates, 358 AFLP markers were scored. The linkage analysis generated 19 large linkage groups, containing 6 or more markers, which covered 1468 cM. The physical size to genetic distance was approximately 11.1 kbp/cM. Segregation of three intersterility gene loci were analysed through mating of the progeny isolates with three tester strains carrying known intersterility genotypes. The loci for the two intersterility genes (S and P) were successfully located in the map. Segregation of the mating type locus was analysed by backcrossing the progeny isolates with their parental strains. The mating type locus could not be located in the map.
authors.affiliation: Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, 75007 Uppsala, Sweden. marten.lind@mykopat.slu.se
authors.forename: Mårten
authors.initials: M
authors.lastname: Lind
authors.forename: Ake
authors.initials: A
authors.lastname: Olson
authors.forename: Jan
authors.initials: J
authors.lastname: Stenlid
mesh_terms: D000363:Agaricales / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D008040:Genetic Linkage; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers