title: [Expression of band 3 protein on erythrocytes of malignant tumor patients and its impact on proliferation of K562 cells].
abstract: OBJECTIVE
  Membrane domain of band 3 protein (mdb3) mediates transmembrane exchange of chloride and bicarbonate, and regulates intracellular pH. It has been found recently that abnormality of CI(-)/HCO3(-) exchange, which mainly leads to change of intracellular pH, may be involved in cell proliferation and apoptosis. This study was to explore expression of band 3 protein on erythrocytes, and its impact on proliferation of K562 cells.
  
  
  METHODS
  Anion transport activity of band 3 protein on erythrocytes of 8 malignant tumor patients was measured using SPQ fluorescent probe. Expression of band 3 protein was detected by Western blot. Plasmid pYD1-mdb3 was constructed, and transfected into K562 cells. Cl- transport activity and proliferation of K562 cells were detected after transfection.
  
  
  RESULTS
  Of the 8 patients, 7 showed increase of anion transport activity on erythrocytes, 5 showed increase of band 3 protein expression. To some extent, expression of mdb3 enhanced proliferation of K562 cells.
  
  
  CONCLUSIONS
  Expression of band 3 protein is enhanced on erythrocytes of some malignant tumors, and might be a candidate marker of malignant tumors.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin, Heilongjiang, 150086, P.R.China.
authors.forename: Shu-Zhi
authors.initials: SZ
authors.lastname: Bai
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Liu
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Xi
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Wang
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Fu
mesh_terms: D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000096:biosynthesis* / Q000235:genetics; D001692:Biological Transport; D049109:Cell Proliferation; D004910:Erythrocyte Membrane / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells / Q000166:cytology / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D011897:Random Allocation; D014162:Transfection
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte