title: Polycomb group protein ezh2 controls actin polymerization and cell signaling.
abstract: Polycomb group protein Ezh2, one of the key regulators of development in organisms from flies to mice, exerts its epigenetic function through regulation of histone methylation. Here, we report the existence of the cytosolic Ezh2-containing methyltransferase complex and tie the function of this complex to regulation of actin polymerization in various cell types. Genetic evidence supports the essential role of cytosolic Ezh2 in actin polymerization-dependent processes such as antigen receptor signaling in T cells and PDGF-induced dorsal circular ruffle formation in fibroblasts. Revealed function of Ezh2 points to a broader usage of lysine methylation in regulation of both nuclear and extra-nuclear signaling processes.
authors.affiliation: Laboratory of Lymphocyte Signaling, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. sui@rockefeller.edu
authors.forename: I-hsin
authors.initials: IH
authors.lastname: Su
authors.forename: Marc-Werner
authors.initials: MW
authors.lastname: Dobenecker
authors.forename: Ephraim
authors.initials: E
authors.lastname: Dickinson
authors.forename: Matthew
authors.initials: M
authors.lastname: Oser
authors.forename: Ashwin
authors.initials: A
authors.lastname: Basavaraj
authors.forename: Raphael
authors.initials: R
authors.lastname: Marqueron
authors.forename: Agnes
authors.initials: A
authors.lastname: Viale
authors.forename: Danny
authors.initials: D
authors.lastname: Reinberg
authors.forename: Christoph
authors.initials: C
authors.lastname: Wülfing
authors.forename: Alexander
authors.initials: A
authors.lastname: Tarakhovsky
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D017951:Antigen Presentation / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology / Q000502:physiology; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins; D000071221:Enhancer of Zeste Homolog 2 Protein; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D063151:Polycomb Repressive Complex 2; D011496:Protein Methyltransferases / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051567:Proto-Oncogene Proteins c-vav; D011948:Receptors, Antigen, T-Cell / Q000819:agonists; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000502:physiology; D014157:Transcription Factors; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism
chemical_list: D000199:Actins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C491488:EED protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D011948:Receptors, Antigen, T-Cell; D012097:Repressor Proteins; C430461:SUZ12 protein, human; D014157:Transcription Factors; C496618:VAV1 protein, human; C496619:Vav1 protein, mouse; D011496:Protein Methyltransferases; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; C498410:Ezh2 protein, mouse; D011495:Histone-Lysine N-Methyltransferase; D063151:Polycomb Repressive Complex 2; D020764:cdc42 GTP-Binding Protein