title: Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein.
abstract: The storage mite, Tyrophagus putrescentiae, is one of the important causes of allergic disorders. Fifteen allergenic components were demonstrated in storage mite by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, but only the group 2 allergen Tyr p 2 has been cloned and characterized. In this study, we attempted to identify and characterize new allergens from T. putrescentiae, which is a dominant species of storage mite in Korea. Expressed sequence tags were analyzed to identify possible storage mite allergens, and the cDNA sequence encoding a protein homologous to fatty acid binding protein, a mite group 13 allergen, was identified and named Tyr p 13. Its deduced amino acid sequence showed 61.1 to 85.3% identity with other mite group 13 allergens. The recombinant protein was expressed in Escherichia coli using a pET 28b vector system, and its allergenicity was investigated by enzyme-linked immunosorbent assay (ELISA). The recombinant allergen was detected in 5 of 78 (6.4%) T. putrescentiae-positive sera tested, and it inhibited 61.9% of immunoglobulin E binding to crude extract at an inhibitor concentration of 10 mug/ml by inhibition ELISA using serum from the patient who showed the strongest reaction by ELISA. In this study, a novel allergen was identified in T. putrescentiae. This allergen could be helpful for more-detailed characterizations of storage mite allergy.
authors.affiliation: Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Kyoung Yong
authors.initials: KY
authors.lastname: Jeong
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Kim
authors.forename: Jae Sik
authors.initials: JS
authors.lastname: Lee
authors.forename: Jongweon
authors.initials: J
authors.lastname: Lee
authors.forename: In-Yong
authors.initials: IY
authors.lastname: Lee
authors.forename: Kyu-Earn
authors.initials: KE
authors.lastname: Kim
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Park
authors.forename: Chein-Soo
authors.initials: CS
authors.lastname: Hong
authors.forename: Han-Il
authors.initials: HI
authors.lastname: Ree
authors.forename: Tai-Soon
authors.initials: TS
authors.lastname: Yong
mesh_terms: D040041:Acaridae / Q000276:immunology*; D000293:Adolescent; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D007073:Immunoglobulin E / Q000276:immunology*; D007723:Korea; D008297:Male; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000485:Allergens; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D011506:Proteins; D011994:Recombinant Proteins; C110921:Tyr p 2 protein, Tyrophagus putrescentiae; D007073:Immunoglobulin E