Comparative study on rapid dot-immunogold staining and two immunogold silver staining assays for diagnosing schistosomiasis japonica.
A fast, specific, sensitive, convenient, and economical rapid-dot-immunogold staining (R-Dot-IGS) assay was used to detect serum antibodies in patients infected with Schistosoma japonicum. The soluble egg antigen of Schistosoma japonicum was added onto microspore membrane. After pre-reacting and blocking, the serum to be detected and sheep anti-human IgG labeled with chloroauric acid were added sequentially. The assay took 15 minutes. For comparison, the dot-immunogold silver staining (Dot-IGSS) and rapid micro-volume Dot-IGSS (RM-Dot-IGSS) assay were also performed. The positive rate to detect the serum of schistosomiasis japonica by the R-Dot-IGS, Dot-IGSS and RM-Dot-IGSS assay was 98%, 98% and 100%, respectively. Samples from 50 healthy controls, 10 cases of clonorchiasis, and 10 cases of paragonimiasis showed negative reactions except for one case of clonorchiasis with RM-Dot-IGSS assay. Compared with Dot-IGSS and RM-Dot-IGSS, R-Dot-IGS assay has similar sensitivity and specificity, but the latter is quicker, simpler, and cheaper. Therefore, R-Dot-IGS is strongly recommended for rapid diagnosis of schistosomiasis japonica both in epidemiological study and in the clinic.
Yisheng
Liu
L
Department of Parasitology, Xuzhou Medical College, Xuzhou, Jiangsu, PR China. liuyisheng52@163.com
Wenping
Du
D
Kuiyang
Zheng
Z
Linlin
Fu
F
Ming
Chen
C
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D016022:Case-Control Studies; D017979:Gold Colloid / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D016622:Silver Staining; D013194:Staining and Labeling
D000909:Antibodies, Helminth; D017979:Gold Colloid