Determination and correlation of the reversed-phase thin-layer chromatographic parameter (Rm) of a series of 3-(4-substituted-1-piperazinyl)-1-substituted-1-phenyl propanol derivatives with their LD50 values.
The experimental Rm values of four series of 3-(4-substituted-1- piperazinyl)-1-substituted-1-phenyl propanol derivatives, previously prepared for quantitative structure-activity relationship studies, were determined using reversed-phased thin-layer chromatography. The Rm values were determined for various concentrations of acetone in water (50, 60, 70, 80 and 90%), and the obtained correlation lines of Rm against proportion of acetone were extrapolated to 100% water The extrapolated Rm values of two of the four series of the 3-(4-benzyl-1-piperazinyl)-1-phenyl propanol derivatives were correlated with LD50 values obtained from 24-hour acute toxicity studies in albino mice. The extrapolated Rm values correlated parabolically with the LD50s.
Osadebe
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences University of Nigeria, Nsukka - Nigeria.
Patience
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D000465:Algorithms; D000818:Animals; D002855:Chromatography, Thin Layer; D007928:Lethal Dose 50; D051379:Mice; D010879:Piperazines / Q000032:analysis* / Q000633:toxicity*
D010879:Piperazines