Odontoblast expression of semaphorin 7A during innervation of human dentin.
Semaphorin 7A (SEMA 7A) is a membrane-anchored member of the semaphorin family of guidance proteins, previously identified in the immune system. Expressed in central and peripheral nervous system during embryonic and post-natal stages, it can mediate neuronal functions by promoting axonal growth. We show here that SEMA 7A is expressed in human odontoblasts in vivo and in vitro and that its expression is correlated with the establishment of dentin-pulp complex terminal innervation . Co-cultures of trigeminal ganglion (TG) with COS cells overexpressing SEMA 7A demonstrate that SEMA 7A can promote the growth of trigeminal nerve fibers. Finally, by RT-PCR and immunochemistry, we show that beta1-integrin, a SEMA 7A putative receptor, is expressed in pulpal nerve fibers but we failed to detect a co-localization between nerves and odontoblasts through these molecules. On the basis of these data, we suggest that SEMA 7A might be a molecule involved in the terminal innervation of the dentin-pulp complex.
Maurin
Jean-Christophe
JC
Laboratoire du Développement des Tissus Dentaires, ESPRI INSERM / EA 1892, IFR 62, Faculté d'Odontologie; UNIV LYON 1, Rue Guillaume Paradin, 69372 Lyon cedex 08, France.
Delorme
Guillaume
G
Machuca-Gayet
Irma
I
Couble
Marie-Lise
ML
Magloire
Henry
H
Jurdic
Pierre
P
Bleicher
Françoise
F
D000293:Adolescent; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D003782:Dental Pulp / Q000166:cytology / Q000294:innervation; D003804:Dentin / Q000294:innervation*; D004622:Embryo, Mammalian / Q000378:metabolism; D058851:GPI-Linked Proteins; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D009420:Nervous System / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D016501:Neurites / Q000502:physiology; D009804:Odontoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014162:Transfection; D012668:Trigeminal Ganglion / Q000166:cytology / Q000502:physiology
D015703:Antigens, CD; D058851:GPI-Linked Proteins; D019012:Integrin beta1; C118753:SEMA7A protein, human; D039961:Semaphorins