Proviral DNA of caprine arthritis encephalitis virus (CAEV) is detected in cumulus oophorus cells but not in oocytes from naturally infected goats.
The aim of this study was to determine whether oocytes taken from ovarian follicles in 123 naturally infected goats were carrying the proviral CAEV genome. Examination of DNA isolated from 190 batches of oocytes with intact cumulus cells and 190 batches of oocytes whose cumulus cells had been removed, taken from follicles of the same ovaries, demonstrated that 42/190 batches of oocytes with intact cumulus cells had the proviral CAEV genome, whereas none of the 190 batches of oocytes without cumulus cells were positive for the provirus. To confirm that the proviral genome was present in the cumulus cells and not in the oocyte cells, 586 oocytes from 56 different ovaries, were separated from their cumulus cells. The DNA was then extracted from each fraction and examined. The purity of the oocyte fraction was verified by searching for granulosa cell-specific mRNA, using RT-PCR; this was negative in all the batches of oocytes in which the cumulus cells had been removed. PCR analysis demonstrated that none of the oocytes without cumulus cells were positive, whereas 22/56 of the batches with cumulus cells were found to be positive. This study clearly demonstrates that despite being surrounded by infected cumulus cells, the oocytes are not infected, and that the enzymatic and mechanical technique for removing the cells surrounding the oocyte, as used in this study, is effective, thus enabling CAEV-free oocytes to be obtained from infected goats.
Ali Al Ahmad
M Z
MZ
Department of Research into the Health Risks and Biotechnology of Reproduction ENVN/DGER, National Veterinary School, BP 40706, 44307 Nantes Cedex 03, France.
Fieni
F
F
Martignat
L
L
Chatagnon
G
G
Baril
G
G
Bouvier
F
F
Chebloune
Y
Y
D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D016180:Lentivirus Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D009865:Oocytes / Q000821:virology*; D006080:Ovarian Follicle / Q000821:virology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004279:DNA, Viral