The eefABC multidrug efflux pump operon is repressed by H-NS in Enterobacter aerogenes.
The Enterobacter aerogenes eefABC locus, which encodes a tripartite efflux pump, was cloned by complementation of an Escherichia coli tolC mutant. E. aerogenes deltaacrA expressing EefABC became less susceptible to a wide range of antibiotics. Data from eef::lacZ fusions showed that eefABC was not transcribed in the various laboratory conditions tested. However, increased transcription from Peef was observed in an E. coli hns mutant. In addition, EefA was detected in E. aerogenes expressing a dominant negative E. coli hns allele.
Masi
Muriel
M
Enveloppe bactérienne, Perméabilité et Antibiotiques, EA2197, Faculté de Médecine, Marseille, France.
Pagès
Jean-Marie
JM
Villard
Claude
C
Pradel
Elizabeth
E
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D021902:Enterobacter aerogenes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics*; D005816:Genetic Complementation Test; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon / Q000502:physiology*; D013489:Suppression, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria