A novel role for myosin II in insulin-stimulated glucose uptake in 3T3-L1 adipocytes.
Insulin-stimulated glucose uptake requires the activation of several signaling pathways to mediate the translocation and fusion of GLUT4 vesicles from an intracellular pool to the plasma membrane. The studies presented here show that inhibition of myosin II activity impairs GLUT4-mediated glucose uptake but not GLUT4 translocation to the plasma membrane. We also show that adipocytes express both myosin IIA and IIB isoforms, and that myosin IIA is recruited to the plasma membrane upon insulin stimulation. Taken together, the data presented here represent the first demonstration that GLUT4-mediated glucose uptake is a myosin II-dependent process in adipocytes. Based on our findings, we hypothesize that myosin II is activated upon insulin stimulation and recruited to the cell cortex to facilitate GLUT4 fusion with the plasma membrane. The identification of myosin II as a key component of GLUT4-mediated glucose uptake represents an important advance in our understanding of the mechanisms regulating glucose homeostasis.
Steimle
Paul A
PA
Department of Biology, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.
Fulcher
F Kent
FK
Patel
Yashomati M
YM
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006706:Homeostasis; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D009124:Muscle Proteins / Q000502:physiology; D024462:Myosin Type II / Q000037:antagonists & inhibitors / Q000502:physiology*
D051275:Glucose Transporter Type 4; D006576:Heterocyclic Compounds, 4 or More Rings; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495693:Slc2a4 protein, mouse; C472645:blebbistatin; D024462:Myosin Type II; D005947:Glucose