Monocytes from dialysis patients exhibit characteristics of senescent cells: does it really mean inflammation?
Hemodialysis treatment induces mononuclear cell activation particularly if cellulosic hemodialysis membrane is used. In normal cells, repeated activation induce a process of accelerate cellular senescence. The aim of the present study was to evaluate whether the mononuclear cell activation associated to hemodialysis with cellulosic membranes favors a process of accelerate senescence in mononuclear cells. Our results show that mononuclear cells from patients dialyzed with cellulosic membranes, exhibit: decrease telomere length, increase percentage of cells CD14dim/CD16bright and increase production of IL-1beta, IL1Ra and IL6 cytokines. After culture in vitro, these cells shown increase susceptibility to undergoing spontaneous apoptosis, that is enhanced by IL-4 and prevented by IL-1beta or LPS. All of these characteristics have been reported associated to senescence of monocytes, and not are observed in cells from controls subjects or patients dialyzed with non-cellulosic membranes, suggesting that hemodialysis with cellulosic membranes induce a process of senescence in mononuclear cells.
Carracedo
Julia
J
Unidad de Investigación, Servicio de Nefrología. Hospital Universitario Reina Sofía, Córdoba, Spain.
Ramirez
Rafael
R
Soriano
Sagrario
S
Alvarez de Lara
Maria Antonia
MA
Rodriguez
Mariano
M
Martin-Malo
Alejandro
A
Aljama
Pedro
P
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects; D016022:Case-Control Studies; D002478:Cells, Cultured; D016922:Cellular Senescence; D002482:Cellulose / Q000009:adverse effects; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000209:etiology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D008567:Membranes, Artificial; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000648:ultrastructure; D010641:Phenotype; D017452:Receptors, IgG / Q000378:metabolism; D006435:Renal Dialysis / Q000009:adverse effects* / Q000295:instrumentation; D016615:Telomere / Q000648:ultrastructure
D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008222:Lymphokines; D008567:Membranes, Artificial; D017452:Receptors, IgG; D002482:Cellulose