Isolation and purification of coumarin compounds from Cortex fraxinus by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of coumarin compounds from Cortex fraxinus, the Chinese herbal drug. n-Butanol-methanol-0.5% acetic acid (5:1.5:5, v/v) was used as the two-phase solvent system. 14.3 mg of fraxin, 26.5 mg of aesculin, 5.8 mg of fraxetin and 32.4 mg of aesculetin with the purity of 97.6, 99.5, 97.2 and 98.7%, respectively were obtained from 150 mg of crude extracts of C. fraxinus in a single run. The structures of the isolated compounds were identified by 1H NMR and 13C NMR.
Liu
Renmin
R
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn
Sun
Qinghua
Q
Sun
Ailing
A
Cui
Jichun
J
D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
D003374:Coumarins; D004365:Drugs, Chinese Herbal