title: Pyruvate but not lactate prevents NADH-induced myoglobin oxidation.
abstract: In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by l-lactate. The antioxidant activity versus H2O2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia-reperfusion injury during infarction or high-intensity exercise.
authors.affiliation: Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education, Wiejska 1, 80-336 Gdansk, Poland.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Olek
authors.forename: Jedrzej
authors.initials: J
authors.lastname: Antosiewicz
authors.forename: Jerzy
authors.initials: J
authors.lastname: Popinigis
authors.forename: Rosita
authors.initials: R
authors.lastname: Gabbianelli
authors.forename: Donatella
authors.initials: D
authors.lastname: Fedeli
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Falcioni
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D006736:Horses; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D019289:Pyruvic Acid / Q000737:chemistry / Q000378:metabolism*; D015427:Reperfusion Injury; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D007773:Lactates; D009211:Myoglobin; D009243:NAD; D019344:Lactic Acid; D019289:Pyruvic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen