Direct identification and quantitative determination of costunolide and dehydrocostuslactone in the fixed oil of Laurus novocanariensis by 13C-NMR spectroscopy.
The fixed oil of Laurus novocanariensis (previously L. azorica) contains mostly glycerides together with minor non-saponifiable compounds. The direct identification and quantitative determination of costunolide and dehydrocostuslactone, two sesquiterpene lactones components of the oil that exhibit biological activities, is described. The analysis was carried out using 13C-NMR spectroscopy (signal acquisition with inverse gated decoupling of protons; diglyme as internal standard) without separation, derivatisation or any sample preparation.
Ferrari
Bernard
B
Université de Corse, Equipe Chimie et Biomasse, UMR CNRS 6134, Route des Sanguinaires, 20000 Ajaccio, France.
Castilho
Paula
P
Tomi
Félix
F
Rodrigues
Ana Isabel
AI
do Ceu Costa
Maria
M
Casanova
Joseph
J
D007783:Lactones / Q000032:analysis*; D027442:Laurus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010938:Plant Oils / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis*
D007783:Lactones; D010938:Plant Oils; D012717:Sesquiterpenes; C083030:dehydrocostus lactone; C002602:costunolide