[Effect of aloe vera polysaccharide on the release of cytokines and nitric oxide in cultured human keratinocytes].
OBJECTIVE
To investigate the effects of polysaccharide extracted from Aloe Barbadensis on the release of cytokines and nitric oxide (NO) in cultured human keratinocytes.


METHODS
The levels of transforming growth factor-alpha (TGF-alpha), TGF-beta1, interleukin-1beta (IL-1beta), IL-6, IL-8, tumor necrosis factor (TNF) and NO in the supernatants of keratinocyte culture in which culture media containing 25, 50, 100, 200, 400 microg/ml, respectively of aloe polysaccharide were assayed. In the control group equal volume of media without the polysaccharide was used.


RESULTS
Compared with control group, the levels of TGF-alpha, TGF-beta1, IL-1beta, IL-6, IL-8 and TNF in the supernatants of cultured keratinocytes were significantly higher when aloe polysaccharide was added (P<0.05 or P<0.01), and they were positively correlated to the concentration of aloe polysaccharide (P<0.01). However, aloe polysaccharide markedly decreased the level of NO in a dose dependent manner (P<0.01).


CONCLUSIONS
Aloe polysaccharide could promote keratinocytes to secrete TGF-alpha, TGF-beta1, IL-1beta, IL-6, IL-8 and TNF, and inhibit the release of NO.
Chen
Xiao-dong
XD
Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China.
Huang
Li-ying
LY
Wu
Bo-yu
BY
Jiang
Qiong
Q
Wang
Zhong-cheng
ZC
Lin
Xin-hua
XH
D000504:Aloe / Q000737:chemistry*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*
D016207:Cytokines; D011134:Polysaccharides; D009569:Nitric Oxide