A new scaleable method for the purification of botulinum neurotoxin type E.
Botulinum neurotoxins belong to the most toxic substances in nature. Well-known as a food poisoning agent for almost two centuries, the beneficial aspects of this bacterial metabolite were rediscovered about 30 years ago. These toxins, which are produced by the anaerobic bacterium Clostridium botulinum are nowadays used to treat a variety of neuro-muscular disorders. The increased demand requires techniques for the production and purification of these toxins on an industrial scale. The method described herein is based on filtration and chromatography procedures only. Precipitation, centrifugation and dialysis steps were consequently excluded to develop a protocol, which can easily be scaled up from the laboratory purification to industrial needs. About 4 mg of BoNT/E were purified from a 10-L batch culture corresponding to an overall recovery of approximately 14%.
Gessler
Frank
F
Institute of Agronomy and Animal Production in the Tropics, Georg-August-University, Kellnerweg 6, 37077 Göttingen, Germany. fgessle@gwdg.de
D000818:Animals; D001905:Botulinum Toxins / Q000096:biosynthesis* / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D003014:Clostridium botulinum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D014462:Ultrafiltration / Q000295:instrumentation
D001905:Botulinum Toxins; C096326:botulinum toxin type E