title: Frog corticotropin-releasing hormone (CRH): isolation, molecular cloning, and biological activity.
abstract: Corticotropin-releasing hormone (CRH) was isolated from the brain of the European green frog, Rana esculenta, by combining HPLC purification with radioimmunoassay (RIA) detection. The amino acid sequence SEEPPISLDLTFHLLREVLEMARAEQIAQQAHSNRKLMDII was identical with the sequence of bullfrog (R. catesbeiana) CRH that was deduced from a cDNA encoding the CRH precursor. Synthetic frog CRH enhanced the release of thyroid-stimulating hormone (TSH) from dispersed bullfrog pituitary cells in a concentration-dependent manner. The TSH-releasing activity of a bullfrog hypothalamic extract was decreased by approximately 45% in the presence of the CRH receptor antagonist, alpha-helical CRH(9-41), suggesting that CRH is one of the main TSH-releasing factors present in the bullfrog hypothalamus.
authors.affiliation: Department of Biology, School of Education, Waseda University, Tokyo, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Okada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ito
authors.forename: Miyoko
authors.initials: M
authors.lastname: Kaneko
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chartrel
authors.forename: J Michael
authors.initials: JM
authors.lastname: Conlon
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Sakae
authors.initials: S
authors.lastname: Kikuyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000502:physiology; D011892:Rana catesbeiana; D011893:Rana esculenta
chemical_list: D003346:Corticotropin-Releasing Hormone