Bovine mater-like NALP9 is an oocyte marker gene.
We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the maternal effect factor MATER. By reverse transcription coupled to polymerase chain reaction, we have analyzed expression of NALP9 in a panel of somatic and gonadic tissues and during in vitro preimplantation development. The transcript was detected exclusively in testis and ovary, and at a remarkably high level in the oocyte from antral follicles. NALP9 mRNA remained present after in vitro maturation and fertilization, and was detected in embryos, but transcription was not reactivated at the maternal to embryo transition. Thus NALP9 is characterized as a novel oocyte marker gene in cattle.
Dalbiès-Tran
Rozenn
R
Physiologie de la Reproduction et des Comportements, UMR 6175 Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique/Université François Rabelais de Tours/Haras Nationaux, Nouzilly, France. dalbies@tours.inra.fr
Papillier
Pascal
P
Pennetier
Sophie
S
Uzbekova
Svetlana
S
Monget
Philippe
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D015152:Blotting, Northern; D002417:Cattle; D018076:DNA, Complementary; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D018076:DNA, Complementary; D004527:Egg Proteins