Study on the interaction between DNA and protein induced by anticancer drug carboplatin.
The interaction of DNA and human serum albumin (HSA) in the presence of anticancer drug carboplatin was studied with piezoelectric quartz crystal impedance (PQCI) and electrochemistry techniques. In the PQCI analysis, the correlative parameters including the frequency (f0), the motional resistance (R(m)), and the static capacitance (C0) in the experiment were obtained and discussed in detail. Additionally, the kinetics parameters of the cross-linking process were investigated and a response kinetics model was deduced. The values of association rate constant k1, dissociation rate constant k(-1) and the reaction equilibrium constant K were estimated to be 1.895 mg/ml(-1) s(-1), 4.7 x 10(-5) s(-1) and 4.03 x 10(4) (mg/ml)-1, respectively. Furthermore, cyclic voltammetry (CV) and electrochemical AC impedance techniques were employed to testify the cross-linking process.
Liu
Shanchao
S
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. shanchaoliu@yahoo.com.cn
Liu
Yingju
Y
Li
Jia
J
Guo
Manli
M
Nie
Lihua
L
Yao
Shouzhuo
S
D016190:Carboplatin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D004563:Electrochemistry; D006801:Humans; D007700:Kinetics; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D011506:Proteins; D012709:Serum Albumin; D004247:DNA; D016190:Carboplatin