Identification of 26RFa from frog brain: a novel hypothalamic neuropeptide with orexigenic activity in mammals.
In the present study, we report the identification, in the frog brain, of a novel neuropeptide, termed 26RFa, that belongs to the RFamide peptide family. The cDNAs encoding the precursors for 26RFa have been characterized in human and rats. In rats, prepro-26RFa mRNA is expressed exclusively in two hypothalamic nuclei involved in the control of feeding behavior. Intracerebroventricular injection of 26RFa in mice induced a dose-dependent increase in food consumption. Taken together, these data indicate that 26RFa is a novel neuropeptide that may have important biological functions in vertebrates.
Chartrel
Nicolas
N
European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, University of Rouen, Mont-Saint-Aignan, France. nicolas.chartrel@univ-rouen.fr
Bruzzone
Federica
F
Dujardin
Cynthia
C
Leprince
Jerome
J
Tollemer
Helene
H
Anouar
Youssef
Y
Vallarino
Mauro
M
Costentin
Jean
J
Vaudry
Hubert
H
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D001921:Brain / Q000502:physiology; D006801:Humans; D007031:Hypothalamus / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D000068797:Orexins; D051381:Rats; D017386:Sequence Homology, Amino Acid
D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; C416987:RFamide peptide; C480638:prepro-26RFa protein, human