title: Crystallization and preliminary X-ray diffraction analysis of importin-alpha complexed with NLS peptidomimetics.
abstract: Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). The study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-alpha was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 A resolution. Preliminary electron density calculations show that the ligands are present in the crystals.
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil. fontes@ibb.unesp.br
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
authors.forename: Trazel
authors.initials: T
authors.lastname: Teh
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Riell
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
authors.forename: Robert F
authors.initials: RF
authors.lastname: Standaert
authors.forename: Bostjan
authors.initials: B
authors.lastname: Kobe
mesh_terms: D003460:Crystallization; D008958:Models, Molecular; D018716:Molecular Mimicry; D019913:Nuclear Localization Signals / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D014961:X-Ray Diffraction; D028901:alpha Karyopherins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D019913:Nuclear Localization Signals; D028901:alpha Karyopherins