title: The eefABC multidrug efflux pump operon is repressed by H-NS in Enterobacter aerogenes.
abstract: The Enterobacter aerogenes eefABC locus, which encodes a tripartite efflux pump, was cloned by complementation of an Escherichia coli tolC mutant. E. aerogenes deltaacrA expressing EefABC became less susceptible to a wide range of antibiotics. Data from eef::lacZ fusions showed that eefABC was not transcribed in the various laboratory conditions tested. However, increased transcription from Peef was observed in an E. coli hns mutant. In addition, EefA was detected in E. aerogenes expressing a dominant negative E. coli hns allele.
authors.affiliation: Enveloppe bactérienne, Perméabilité et Antibiotiques, EA2197, Faculté de Médecine, Marseille, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Masi
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Pagès
authors.forename: Claude
authors.initials: C
authors.lastname: Villard
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Pradel
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D021902:Enterobacter aerogenes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics*; D005816:Genetic Complementation Test; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon / Q000502:physiology*; D013489:Suppression, Genetic
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria