[Immunogenicity of bovine jugular vein conduits treated with different cross-linking methods].
OBJECTIVE
To evaluate the immunogenicity of bovine jugular vein conduits (BJVCs) treated with different cross-linking methods.


METHODS
The BJVCs were treated with glutaradehyde (GA), dye-mediated photooxidation (DMP) and polyepoxy compound (PC) (n = 10). The tissue homogenates obtained from BJVCs treated with PC, GA, DMP, and fresh BJVCs, were mixed with the complete Freand adjavant to form the emulsive antigen, which were used to immunize rabbits correspondently. The antibody concentrations to BJVCs in those rabbits' serum were measured by counter double immuno diffusion. The immunologic responses to the BJVCs in different groups were measured with Western blotting.


RESULTS
The positive bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with antigens of fresh BJVCs, but no bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with those antigens of the BJVCs treated with GA, DMP, and PC in Western blotting.


CONCLUSIONS
The immunogenicity of BJVCs treated with PC, DMP, and GA can be reduced significantly and meet the clinical standard.
Wang
Hui
H
Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Hu
Jian-guo
JG
Wu
Zhong-shi
ZS
Hu
Tei-hui
TH
Deng
You-hua
YH
Feng
Yao-guang
YG
Jiang
Zhi-bing
ZB
Yang
Yan-qing
YQ
D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002417:Cattle; D003432:Cross-Linking Reagents; D005976:Glutaral / Q000494:pharmacology; D007601:Jugular Veins / Q000276:immunology*; D019736:Prostheses and Implants; D011817:Rabbits; D011897:Random Allocation
D003432:Cross-Linking Reagents; D005976:Glutaral