[Cloning and characterization of new genes of Schistosoma japonicum].
OBJECTIVE
To clone and characterize new genes of Schistosoma japonicum, Sj, and to provide efficient vaccine candidates.


METHODS
Sj adult cDNA library was screened with rabbit sera raised against male worm soluble antigen. The inserted cDNA fragments from the positively selected clones were amplified with PCR and further sequenced, as well as characterized through internet NCBI GenBank software.


RESULTS
Eleven positive clones were obtained and two were verified by GenBank as new, including a novel gene designated as Sj-P8 (GenBank accession No. AF517843) and a new partial cDNA of Sj myosin (GenBank accession No. AY770506). The two new genes encoded a transmembrane protein of 75 amino acids and a myosin protein fragment of 212 amino acids respectively.


CONCLUSIONS
The newly obtained genes may provide useful information for the research on Sj vaccine.
Chen
Yu-xiao
YX
Department of Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China. yuxiaochen008@yahoo.com.cn
Tang
Lian-fei
LF
Zhang
Jie
J
Yuan
Shi-shan
SS
Zeng
Xian-fang
XF
Yi
Xin-yuan
XY
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D015723:Gene Library; D017238:Genes, Helminth / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics*; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014614:Vaccines, Synthetic
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D014614:Vaccines, Synthetic