Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts.
A variety of procedures are available to detect parasite eggs or oocysts in feces. This study compared the efficacy of simple flotation, a commercial assay, and various centrifugation techniques and three common flotation solutions. Results indicate that centrifugation consistently recovered more eggs than other methods. Proper technique is critical, including ensuring that the specific gravity of the flotation solution is correct and allowing the sample to stand for a sufficient amount of time before examining the coverslip. Because of the zoonotic health risks of many companion animal parasites, veterinarian and their staff should better utilize fecal examinations in their routine diagnostic plan.
Dryden
M W
MW
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Payne
P A
PA
Ridley
R
R
Smith
V
V
D000722:Ancylostoma / Q000302:isolation & purification; D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D006374:Helminthiasis, Animal / Q000175:diagnosis* / Q000469:parasitology; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary*; D011237:Predictive Value of Tests; D017166:Toxocara canis / Q000302:isolation & purification; D014258:Trichuris / Q000302:isolation & purification