Detection of T lymphocyte subsets of children with Helicobacter pylori infection.
OBJECTIVE
To study the transformation of T lymphocyte subsets in children with Heliobacter pylori (H pylori) infection.


METHODS
The H pylori infection status were determined by a combination of ELISA and Western blot (immunoblot) technique in 98 children and T lymphocyte subsets in peripheral blood were determined by flow cytometrical analysis.


RESULTS
There were 75 children positive with H pylori infection and 23 negative in 98 children. Comparing the proportion of peripheral blood T lymphocytic subsets in children with H pylori infection and without H pylori infection, it was found that a higher proportion of CD4 T-cells in infected children (39.02+/-7.71 vs 34.25+/-10.73, t = 2.246,P<0.05) and higher value of CD4 to CD8 T-cells ratio (1.51+/-0.52 vs 1.25, t = 2.104, P<0.05) were present, but there were not significant differences in CD3 T-cells and CD8 T-cells (73.11+/-10.02 vs 69.49+/-17.08, 27.22+/-6.07 vs 28.27+/-8.67, P>0.05).


CONCLUSIONS
Th1 cell-mediated immune responses may be induced by H pylori infection in children.
Wang
Lin-Lin
LL
Department of Pediatrics, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. wll276@yahoo.com.cn
Shan
Qing-Wen
QW
Jiang
Hai-Xing
HX
Tran
Dinh-Binh
DB
Qin
Xue
X
Xie
Xiang-Zhi
XZ
Liang
Dan-Mei
DM
D000293:Adolescent; D002648:Child; D005260:Female; D016481:Helicobacter Infections / Q000097:blood*; D016480:Helicobacter pylori; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*