title: Alteromonas addita sp. nov.
abstract: On the basis of phenotypic, genotypic characteristics and analysis of 16S rRNA gene sequences, a novel species belonging to the genus Alteromonas is described. A non-pigmented, motile, Gram-negative bacterium designated R10SW13(T) was isolated from sea water samples collected in Chazhma Bay (Sea of Japan, Pacific Ocean). The novel organism mainly grew between 4 degrees C and 37 degrees C, was neutrophilic and slightly halophilic, tolerating up to 10 % NaCl. Strain R10SW13(T) was haemolytic and was able to degrade starch and Tween 80 and to degrade gelatin and agar weakly, but did not degrade casein. Phosphatidylethanolamine (44.3 +/- 0.9 %) and phosphatidylglycerol (55.7 +/ -0.9 %) were the predominant phospholipids. The major fatty acids formed were typical for the genus Alteromonas, including 16 : 0, 16 : 1omega-7 and 18 : 1omega-7. The G + C content of the DNA was 43.4 mol%. DNA-DNA hybridization experiments showed 38-53 % binding with the DNAs of type strains of phylogenetically related species of the genus Alteromonas, namely: Alteromonas macleodii, Alteromonas marina, Alteromonas stellipolaris, Alteromonas litorea, 'Alteromonas macleodii subsp. fijiensis' and 'Alteromonas infernus'. Based on these results, a novel species, Alteromonas addita sp. nov., is proposed, with strain R10SW13(T) (=KMM 3600(T) = KCTC 12195(T) = LMG 22532(T)) as the type strain.
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.|Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.affiliation: School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia.
authors.forename: John P
authors.initials: JP
authors.lastname: Bowman
authors.affiliation: S. N. Winogradsky Institute of Microbiology of the Russian Academy of Sciences, 117312 Moscow, Russian Federation.
authors.forename: Anatoly M
authors.initials: AM
authors.lastname: Lysenko
authors.affiliation: Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041, Vladivostok, Russian Federation.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
authors.forename: Nataliya M
authors.initials: NM
authors.lastname: Gorshkova
authors.affiliation: V. I. Il'ichev Pacific Oceanological Institute of the Far-Eastern Branch of the Russian Academy of Sciences, Baltiiskaya Str. 43, 690017, Vladivostok, Russian Federation.
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Sergeev
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
mesh_terms: D000362:Agar / Q000378:metabolism; D020591:Alteromonas / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005780:Gelatin / Q000378:metabolism; D020459:Genes, rRNA; D005840:Gentian Violet; D006131:Growth Inhibitors / Q000494:pharmacology; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D007564:Japan; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010619:Phenazines; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological; D011136:Polysorbates / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D006131:Growth Inhibitors; D010619:Phenazines; D010743:Phospholipids; D010860:Pigments, Biological; D011136:Polysorbates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005780:Gelatin; D000362:Agar; D005840:Gentian Violet