Direct fluorometric analysis of a newly synthesised fluorescein-labelled marker for glomerular filtration rate.
There is an obvious and growing medical need for an accurate determination of kidney function in the diagnosis and management of renal diseases. The glomerular filtration rate (GFR) is the accepted gold standard measurement of kidney function. Several approaches to estimate the GFR are available, but most of them are inconvenient and, therefore, of limited acceptance. A new method of quantification with fluorescein-isothiocyanate (FITC) sinistrin (FS), a novel GFR marker, has been evaluated. The method is based on the fluorescence label of FS and can be performed with a standard fluorometer. To control the interference of protein with the fluorescence signal, a calibration function was developed. The accuracy of the fluorometric method established is comparable to the so-called "gold standard" of enzymatic determination of polyfructosan. Moreover, FS is easy to handle and requires low-cost instruments. Our results demonstrate the potential of the direct fluorometric analysis of the new FITC-labelled marker of being a precise, simple, rapid and cost-effective method for diagnosing disturbed kidney function and monitoring its treatment efficacy. The dramatic decrease in analytical effort will result in a significantly higher acceptability of GFR determination.
Pill
Johannes
J
Roche Diagnostics GmbH, Sandhofer Str. 116, 68305, Mannheim, Germany. johannes.pill@roche.com
Kloetzer
Hans-Martin
HM
Issaeva
Oxana
O
Kraenzlin
Bettina
B
Deus
Carsten
C
Kraemer
Uwe
U
Sadick
Maliha
M
Fiedler
Fritz
F
Gretz
Norbert
N
D000818:Animals; D002138:Calibration; D019793:Fluorescein / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D005919:Glomerular Filtration Rate; D008297:Male; D009844:Oligosaccharides / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005452:Fluoresceins; D009844:Oligosaccharides; C576250:fluorescein-isothiocyanate sinistrin; D019793:Fluorescein