title: Prevalence of chromosome defects in azoospermic and oligoastheno-teratozoospermic South Indian infertile men attending an infertility clinic.
abstract: The prevalence of chromosomal abnormalities in azoospermic and oligoastheno-teratozoospermic infertile men of South Indian origin undergoing assisted reproductive technologies was evaluated. In addition, the study aimed to investigate new abnormal karyotypes involving autosomes in azoospermia and sex chromosomes in oligoastheno-teratozoospermic individuals that are supposed to be rare. Metaphase chromosomes of 744 infertile men, including 272 men with azoospermia and 472 men with oligoastheno-teratozoospermia (OAT), were analysed using Giemsa-trypsin-Giemsa banding and fluorescence in-situ hybridization (FISH) wherever necessary. Chromosomal abnormalities were observed in 59 (7.9%) individuals of the total studied population. Among these, 30 out of 272 (11.0%) azoospermic men and 29 out of 472 (6.1%) infertile men with OAT showed chromosomal abnormalities. A strong and statistically significant association (OR = 1.89; P = 0.0235) of chromosomal abnormalities and sex chromosome abnormalities (OR = 4.29; P = 0.001) with azoospermia when compared with OAT was observed. In addition, six autosomal abnormalities associated with azoospermia and two abnormalities involving Y chromosome, which include a novel karyotype (mos 46,XY/51,XYYYYYY) in OAT individuals, were detected.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad-500007, India.
authors.forename: K Lakshmi
authors.initials: KL
authors.lastname: Rao
authors.forename: K Arvind
authors.initials: KA
authors.lastname: Babu
authors.forename: M K
authors.initials: MK
authors.lastname: Kanakavalli
authors.forename: V V
authors.initials: VV
authors.lastname: Padmalatha
authors.forename: Mamata
authors.initials: M
authors.lastname: Deenadayal
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D041322:Chromosomes, Human, Y / Q000235:genetics; D000013:Congenital Abnormalities / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007194:India / Q000453:epidemiology; D007248:Infertility, Male / Q000235:genetics*; D007621:Karyotyping; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics*; D015995:Prevalence; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000002:abnormalities*