title: Endurance training increases lactate transport in male Zucker fa/fa rats.
abstract: The purpose of this study was to investigate the effect of endurance training (10 weeks) on previously reported alterations of lactate exchange in obese Zucker fa/fa rats. We used sarcolemmal vesicles to measure lactate transport capacity in control sedentary rats, Zucker (fa/fa), and endurance trained Zucker (fa/fa) rats. Monocarboxylate transporter (MCT) 1 and 4 content was measured in sarcolemmal vesicles and skeletal muscle. Training increased citrate synthase activity in soleus and in red tibialis anterior, and improved insulin sensitivity measured by intraperitoneal glucose tolerance test. Endurance training increased lactate influx in sarcolemmal vesicles at 1 mM of external lactate concentration and increased MCT1 expression on sarcolemmal vesicles. Furthermore, muscular lactate level was significantly decreased after training in red tibialis anterior and extensor digitorum longus. This study shows that endurance training improves impairment of lactate transport capacity that is found in insulin resistance state like obesity and type 2 diabetes.
authors.affiliation: Département de Physiologie des Interactions, EA 701 Muscles et Pathologies chroniques, Institut de biologie, CS 89508, 34060 Montpellier cedex 2, France. lore_metz@hotmail.com
authors.forename: Lore
authors.initials: L
authors.lastname: Metz
authors.forename: Marianne
authors.initials: M
authors.lastname: Vermaelen
authors.forename: Karen
authors.initials: K
authors.lastname: Lambert
authors.forename: Christophe
authors.initials: C
authors.lastname: Broca
authors.forename: Pascal
authors.initials: P
authors.lastname: Sirvent
authors.forename: Eric
authors.initials: E
authors.lastname: Raynaud
authors.forename: Jacques
authors.initials: J
authors.lastname: Mercier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D001835:Body Weight; D005951:Glucose Tolerance Test; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal; D051381:Rats; D011924:Rats, Zucker
chemical_list: D019344:Lactic Acid