Adhesion of monocyte-derived dendritic cells to human umbilical vein endothelial cells in flow field decreases upon maturation.
Dendritic cells (DC) are sentinels of the immune system. They and their precursors undergo complex migration to perform their function in vivo. Binding of DC to vascular endothelial cells in a flow field has not been investigated. We therefore determined adhesion of DC and their precursors, MO, to human umbilical vein endothelial cells (HUVECs) under various shear stresses by using a flow chamber system. The results showed that the binding was reduced with developmental stages of DC, which partially depended on CD11a and cell surface charges. The data had potential relevance for anti-cancer immunotherapy strategies favoring the application of mDC.
Jiang
Yuhui
Y
Department of Biophysics, Peking University Health Science Center, Beijing, China.
Zeng
Zhu
Z
Sun
Dagong
D
Ka
Weibo
W
Wen
Zongyao
Z
D000911:Antibodies, Monoclonal / Q000737:chemistry; D040881:CD11a Antigen / Q000096:biosynthesis; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000166:cytology; D009000:Monocytes / Q000166:cytology*; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology*
D000911:Antibodies, Monoclonal; D040881:CD11a Antigen