title: An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection.
abstract: This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5-iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 mM phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Campus de Espinardo, Murcia, Spain. tpr@um.es
authors.forename: Tomás
authors.initials: T
authors.lastname: Pérez-Ruiz
authors.forename: Carmen
authors.initials: C
authors.lastname: Martínez-Lozano
authors.forename: Antonio
authors.initials: A
authors.lastname: Sanz
authors.forename: Raquel
authors.initials: R
authors.lastname: Galera
mesh_terms: D000821:Animal Feed / Q000032:analysis; D013956:Antithyroid Agents / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013889:Thiouracil / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine
chemical_list: D013956:Antithyroid Agents; C060320:6-phenylthiouracil; D013889:Thiouracil