title: Stimulation of midgut stem cell proliferation and differentiation by insect hormones and peptides.
abstract: Stem cells derived from midguts of the caterpillar, Spodoptera littoralis, can be induced to multiply and differentiate in vitro. Ecdysone (E) and 20-hydroxyecdysone (20E) had a concentration-dependent effect: E was more active in cell proliferation and 20E in differentiation. Ecdysteroid receptors in midgut stem cell nuclei were stained with the antibody 9B9. In addition, alpha-arylphorin and four midgut differentiation factors (MDF) specifically stimulated proliferation and differentiation of stem cells, respectively. The activity of a panel of peptide growth factors and hormones on growth and metamorphosis of the insect midgut is discussed.
authors.affiliation: Laboratory of Agrozoology, Ghent University, Belgium. guy.smagghe@ugent.be
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.forename: Wendy
authors.initials: W
authors.lastname: Vanhassel
authors.forename: Clarina
authors.initials: C
authors.lastname: Moeremans
authors.forename: Daan
authors.initials: D
authors.lastname: De Wilde
authors.forename: Shintaro
authors.initials: S
authors.lastname: Goto
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: Loeb
authors.forename: Michael B
authors.initials: MB
authors.lastname: Blackburn
authors.forename: Raziel S
authors.initials: RS
authors.lastname: Hakim
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004440:Ecdysone / Q000502:physiology; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D007301:Insect Hormones / Q000502:physiology*; D019476:Insect Proteins / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D018411:Spodoptera / Q000187:drug effects / Q000254:growth & development*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; D010455:Peptides; D004440:Ecdysone