title: Immunolocalization of fibroblast growth factor-2 (FGF-2) in the developing root and supporting structures of the murine tooth.
abstract: Epithelio-mesenchymal interactions are active during the development of the root of the tooth and are regulated by a variety of growth factors, such as fibroblast growth factors. FGF-2, 3, 4, and 8 have all been shown to play a role in the development of the crown of the tooth, but less is known about the factors that govern root formation, particularly FGF-2. The aim of this study was thus to elucidate the spatial and temporal expression of FGF-2 in the root of the developing tooth, as this growth factor is believed to be a mediator of epithelio-mesenchymal interactions. Parasagittal sections of the maxillary and mandibular arches of post-natal mice were utilized and the roots of the molar teeth were studied. Immunocytochemistry utilizing an antibody to FGF-2 was performed on sections of teeth at various stages of development. Intense immunostaining for FGF-2 was observed in differentiating odontoblasts at the apical end of the tooth and in the furcation zone of the developing root at all the stages examined. FGF-2 localization was also observed in cementoblasts on post-natal days 16, 20 and 24. The pattern of localization of FGF-2 in the developing root suggests that this growth factor may participate in the signaling network associated with root development.
authors.affiliation: Embryonic Development and Differentiation Research Programme, School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Parktown, 2193 Johannesburg, South Africa.
authors.forename: A K
authors.initials: AK
authors.lastname: Madan
authors.forename: Beverley
authors.initials: B
authors.lastname: Kramer
mesh_terms: D000565:Ameloblasts / Q000502:physiology; D000818:Animals; D003782:Dental Pulp / Q000254:growth & development / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008334:Mandible / Q000254:growth & development / Q000378:metabolism; D008437:Maxilla / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D009804:Odontoblasts / Q000378:metabolism; D014070:Tooth / Q000254:growth & development / Q000378:metabolism*; D014092:Tooth Root / Q000254:growth & development / Q000378:metabolism*; D020390:Tooth Socket / Q000254:growth & development / Q000378:metabolism
chemical_list: D016222:Fibroblast Growth Factor 2