Expressed genes in regenerating rat liver after partial hepatectomy.
OBJECTIVE
To reveal the liver regeneration (LR) and its control as well as the occurrence of liver disease and to study the gene expression profiles of 551 genes after partial hepatectomy (PH) in regenerating rat livers.


METHODS
Five hundred and fifty-one expressed sequence tags screened by suppression subtractive hybridization were made into an in-house cDNA microarray, and the expressive genes and their expressive profiles in regenerating rat livers were analyzed by microarray and bioinformatics.


RESULTS
Three hundred of the analyzed 551 genes were up- or downregulated more than twofolds at one or more time points during LR. Most of the genes were up- or downregulated 2-5 folds, but the highest reached 90 folds of the control. One hundred and thirty-nine of them showed upregulation, 135 displayed downregulation, and up or down expression of 26 genes revealed a dependence on regenerating livers. The genes expressed in 24-h regenerating livers were much more than those in the others. Cluster analysis and generalization analysis showed that there were at least six distinct temporal patterns of gene expression in the regenerating livers, that is, genes were expressed in the immediate early phase, early phase, intermediate phase, early-late phase, late phase, terminal phase.


CONCLUSIONS
In LR, the number of down-regulated genes was almost similar to that of the upregulated genes; the successively altered genes were more than the rapidly transient genes. The temporal patterns of gene expression were similar 2 and 4 h, 12 and 16 h, 48 and 96 h, 72 and 144 h after PH. Microarray combined with suppressive subtractive hybridization can effectively identify the genes related to LR.
Xu
Cun-Shuan
CS
College of Life Science, Henan Normal University, Xinxiang 453007, Henan Province, China. xucs@x263.net.cn
Chang
Cui-Fang
CF
Yuan
Jin-Yun
JY
Li
Wen-Qiang
WQ
Han
Hong-Peng
HP
Yang
Ke-Jin
KJ
Zhao
Li-Feng
LF
Li
Yu-Chang
YC
Zhang
Hui-Yong
HY
Rahman
Salman
S
Zhang
Jing-Bo
JB
D000818:Animals; D020869:Gene Expression Profiling; D006498:Hepatectomy / Q000379:methods; D008099:Liver / Q000502:physiology* / Q000601:surgery; D008115:Liver Regeneration / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley