title: Characterization of a molt-inhibiting hormone (MIH) receptor in the Y-organ of the kuruma prawn, Marsupenaeus japonicus.
abstract: To characterize a receptor for molt-inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross-linking experiments using radiolabeled recombinant MIH ([(125)I]rMIH). The specific binding of [(125)I]rMIH was found in the membrane fraction of the Y-organ (K(d) = 4.76 x 10(-10) M and B(max) = 5.51 x 10(-12) M). Chemical cross-linking between [(125)I]rMIH and the membrane fraction of the Y-organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Asazuma
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Katayama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohira
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D004702:Endocrine Glands / Q000378:metabolism*; D007447:Invertebrate Hormones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology
chemical_list: D007447:Invertebrate Hormones; D009479:Neuropeptides; C012445:molt-inhibiting hormone