Intraocular lens-capsular bag imaging with ultrahigh-resolution optical coherence tomography Pseudophakic human autopsy eyes.
OBJECTIVE
To compare in vitro ultrahigh-resolution optical coherence tomography (UHR OCT) cross-sectional images of the pseudophakic human autopsy eye with histology to evaluate the potential of this imaging technique for enhanced visualization of the anterior segment, especially the capsular bag, intraocular lens (IOL), and posterior capsule opacification (PCO) formation after cataract surgery.


METHODS
Department of Medical Physics and Department of Ophthalmology, University of Vienna, Vienna, Austria, and Department of Oral and Maxillofacial Surgery Institute of Dentistry, University of Turku, Turku, Finland.


METHODS
Ultrahigh-resolution OCT images were acquired from 7 pseudophakic human autopsy eyes using 1.4 microm axial x 3.0 microm transverse resolution. The axial resolution with UHR OCT is 1.4 microm compared to 10.0 microm with the commercially available OCT. Plastic-embedded histologic sections were obtained in precise alignment with the OCT tomograms.


RESULTS
Ultrahigh-resolution OCT cross-sectional tomograms corresponded to the histologic sections. With the wavelength used (800 nm), the anterior and posterior capsules, area of lens epithelial cell growth and extracellular matrix proliferation, and IOL could be clearly visualized. The extent of capsular bag adhesion to the IOL could be detected, as well as the amount of PCO formation.


CONCLUSIONS
The improved resolution makes UHR OCT a powerful tool in anterior segment imaging and evaluation of the capacity of IOL materials and models to induce capsular bag adhesion. Ultrahigh-resolution OCT may also help in determining the area of origin of PCO after cataract surgery.
Linnola
Reijo J
RJ
PlusHealth-Doctors Ltd., Vaasa, Finland.
Findl
Oliver
O
Hermann
Boris
B
Sattmann
Harald
H
Unterhuber
Angelika
A
Happonen
Risto-Pekka
RP
Drexler
Wolfgang
W
D001344:Autopsy; D002386:Cataract / Q000175:diagnosis; D003941:Diagnostic Techniques, Ophthalmological; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000473:pathology*; D019654:Lens Implantation, Intraocular; D007910:Lenses, Intraocular; D018918:Phacoemulsification; D011183:Postoperative Complications / Q000175:diagnosis; D019591:Pseudophakia / Q000175:diagnosis*; D041623:Tomography, Optical Coherence / Q000379:methods*