title: In situ localization of sulphur in the thioautotrophic symbiotic model Lucina pectinata (Gmelin, 1791) by cryo-EFTEM microanalysis.
abstract: BACKGROUND
  Lucina pectinata is a large tropical lucinid known to harbour sulphide-oxidizing bacteria in specialized gill cells. Conventional TEM (transmission electron microscopy) has shown that bacteriocytes also harbour visibly 'empty' vesicles whose chemical content remains, to date, only roughly determined.
  
  
  RESULTS
  In the present study, L. pectinata gill tissues were cryo-fixed as fast as possible by performing high-pressure freezing before a freeze-substitution process and finally performing a cryo-embedding in Lowicryl. Ultrathin sections were then used for a cryo-EFTEM (where EFTEM stands for energy-filtered TEM) microanalysis. Results show that bacteriocytes within the gill tissues contain elemental sulphur in small vesicles produced by the host itself. In instances of sporadic depletion of sulphur in the environment, such structures may act as energy sources for bacterial endosymbionts.
  
  
  CONCLUSIONS
  The cryo-EFTEM techniques represent (i) the only method used to date to locate and preserve sulphur at the cellular level and (ii) a powerful tool for sulphur metabolism analysis in thioautotrophic symbiont relationships.
authors.affiliation: UMR 7138 Systématique, Adaptation, Evolution, Université Pierre et Marie Curie, Bât A, 4ème étage, 7, Quai Saint Bernard 75005, Paris, France. jean-pierre.lechaire@snv.jussieu.fr
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Lechaire
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Frébourg
authors.forename: Françoise
authors.initials: F
authors.lastname: Gaill
authors.forename: Olivier
authors.initials: O
authors.lastname: Gros
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000382:microbiology; D016616:Cryoultramicrotomy / Q000379:methods*; D005615:Freezing; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D046032:Microscopy, Energy-Filtering Transmission Electron / Q000379:methods*; D013455:Sulfur / Q000032:analysis*; D013559:Symbiosis
chemical_list: D013455:Sulfur