title: Simultaneous measurement of multiple radiation-induced protein expression profiles using the Luminex(TM) system.
abstract: Space flight results in the exposure of astronauts to a mixed field of radiation composed of energetic particles of varying energies, and biological indicators of space radiation exposure provides a better understanding of the associated long-term health risks. Current methods of biodosimetry have employed the use of cytogenetic analysis for biodosimetry, and more recently the advent of technological progression has led to advanced research in the use of genomic and proteomic expression profiling to simultaneously assess biomarkers of radiation exposure. We describe here the technical advantages of the Luminex(TM) 100 system relative to traditional methods and its potential as a tool to simultaneously profile multiple proteins induced by ionizing radiation. The development of such a bioassay would provide more relevant post-translational dynamics of stress response and will impart important implications in the advancement of space and other radiation contact monitoring.
authors.affiliation: Wyle Laboratories, Houston, TX 77058, USA.
authors.forename: N
authors.initials: N
authors.lastname: Desai
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: K
authors.initials: K
authors.lastname: George
authors.forename: S R
authors.initials: SR
authors.lastname: Gonda
authors.forename: F A
authors.initials: FA
authors.lastname: Cucinotta
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D001681:Biological Assay; D015415:Biomarkers; D016207:Cytokines / Q000302:isolation & purification; D005434:Flow Cytometry; D017404:In Situ Hybridization, Fluorescence; D008163:Luminescent Measurements; D008863:Microspheres; D040901:Proteomics; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000379:methods*; D011839:Radiation, Ionizing; D013026:Space Flight
chemical_list: D000906:Antibodies; D015415:Biomarkers; D016207:Cytokines