Cloning and expression analysis of Zmglp1, a new germin-like protein gene in maize.
The cDNA and genomic DNA of a green tissue-specific gene were cloned from maize (Zea mays L.) using cDNA-amplified fragment length polymorphism (cDNA-AFLP) and library screening. The deduced protein was highly similar to Hordeum vulgare germin-like protein 1 (HvGLP1), and the maize gene was therefore designated Zmglp1. Northern blot specifically detected the mRNA of Zmglp1 in young whorl leaves at the early-whorl stage. However, at the late-whorl, tassel, and silk stages, Zmglp1 transcripts were highly abundant in young whorl leaves; less abundant in mature leaves, young tassels, and cobs; and not detectable in roots, immature kernels, and stalks. RNA in situ hybridization revealed that Zmglp1 expressed only in mesophyllous, phloem, and guard cells in the young whorl leaves. Deletion analysis of the promoter in transgenic Arabidopsis resulted in the identification of several regions containing important regulatory cis-elements controlling the expression levels and circadian rhythm-oscillated patterns of Zmglp1.
Fan
Zhanmin
Z
Peking-Yale Joint Research Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.
Gu
Hongya
H
Chen
Xiaowei
X
Song
Hui
H
Wang
Qian
Q
Liu
Meihua
M
Qu
Li-Jia
LJ
Chen
Zhangliang
Z
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018744:DNA, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins