The distribution of inhibitory and excitatory synapses on single, reconstructed jaw-opening motoneurons in the cat.
In a previous study, we reported that the distribution of inhibitory input, in contrast to excitatory input, decreased somatofugally along dendrites of cat jaw-closing alpha-motoneurons [J Comp Neurol 414 (1999) 454]. The present study examined the distribution of GABA, glycine, and glutamate immunopositive boutons covering horseradish peroxidase-labeled cat jaw-opening motoneurons. The motoneurons were divided into four compartments: the soma, and primary, intermediate, and distal dendrites. Ninety-seven percent of the total number of studied boutons had immunoreactivity for at least one of the three amino acids. The proportion of boutons immunoreactive for GABA and/or glycine was lower than the proportion of boutons immunoreactive for glutamate. Boutons immunoreactive to glycine alone were more numerous than boutons double-labeled for GABA and glycine, which, in turn, occurred more frequently than boutons immunoreactive to GABA alone. The percentage synaptic covering (proportion of membrane covered by synaptic boutons) of the putatively excitatory (glutamate containing) and putatively inhibitory (GABA and/or glycine containing) boutons decreased somatofugally along the dendrites. Such systematic variations were not seen in the packing density (number of boutons per 100 microm(2)); the packing density showed a distinct drop between the soma and primary dendrites but did not differ significantly among the three dendritic compartments. Overall, the packing density was slightly higher for the putatively excitatory boutons than for the inhibitory ones. When taken together with previous analyses of jaw-closing alpha-motoneurons the present data on jaw-opening alpha-motoneurons indicate that the two types of neuron differ in regard to the nature of synaptic integration in the dendritic tree.
Shigenaga
Y
Y
Department of Oral Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Jung-Gu, Daegu 700-412, Korea. shigenaga@knu.ac.kr
Moritani
M
M
Oh
S J
SJ
Park
K P
KP
Paik
S K
SK
Bae
J Y
JY
Kim
H N
HN
Ma
S K
SK
Park
C W
CW
Yoshida
A
A
Ottersen
O P
OP
Bae
Y C
YC
D027322:Amino Acid Transport System X-AG / Q000378:metabolism; D000818:Animals; D002415:Cats; D003712:Dendrites / Q000648:ultrastructure; D005998:Glycine / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007568:Jaw / Q000294:innervation / Q000502:physiology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D009046:Motor Neurons / Q000502:physiology* / Q000648:ultrastructure; D009433:Neural Inhibition / Q000502:physiology*; D017729:Presynaptic Terminals / Q000648:ultrastructure; D013569:Synapses / Q000145:classification* / Q000502:physiology* / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D027322:Amino Acid Transport System X-AG; D005680:gamma-Aminobutyric Acid; D005998:Glycine