The role of lipid rafts in LPS-induced signaling in a macrophage cell line.
The significance of lipid rafts in lipopolysaccharide (LPS) signaling in macrophages was studied through isolation of them by gradient centrifugation and subsequent visualization of signal molecules using antibodies. LPS signaling is initiated by binding to Toll-like receptor-4 (TLR4) and the co-receptor CD-14, leading to activation of downstream targets, such as MAP kinases. In this study, we show that LPS causes translocation of CD-14 and MAP kinases (ERK-2 and p38) to lipid rafts in the macrophage cell line RAW 264.7. The adaptor proteins MyD88 and Gab-2, on the other hand, were not detected in the lipid raft fractions. These results indicate that lipid rafts play a role in LPS-induced signaling in macrophages.
Olsson
Sandra
S
Department of Experimental Medical Research, Lund University, BMC, B12, SE 22184 Lund, Sweden.
Sundler
Roger
R
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D021962:Membrane Microdomains / Q000502:physiology*; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4; D048049:Extracellular Signal-Regulated MAP Kinases