title: Quantitating adenylate nucleotides in diverse organisms.
abstract: Quantitation of cellular adenylate levels (i.e., ATP, ADP, AMP) has widespread applications in physiological, metabolic and energetic studies. We have compared classical adenylate extraction procedures (i.e., perchloric acid, boiling) with a previously unreported proteinase K-based extraction technique. Our results suggest that all three techniques are comparable in soft animal tissue, but proteinase K-based extractions consistently generated higher adenylate yields from a broad range of organisms, particularly those containing a cell wall (e.g., alga, bacteria, fungi, plant).
authors.affiliation: Biology Department, 315 Penn Street, Rutgers, The State University of New Jersey, Camden, NJ 08102, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Napolitano
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Shain
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000302:isolation & purification; D000249:Adenosine Monophosphate / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002696:Chlamydomonas / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019286:Endopeptidase K / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry; D031315:Geranium / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry; D010472:Perchlorates
chemical_list: D000227:Adenine Nucleotides; D010472:Perchlorates; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019286:Endopeptidase K