title: Impaired induction of cystatin S gene expression by isoproterenol in the submandibular gland of hypophysectomized rats.
abstract: Cystatin S, an inhibitor of cysteine proteases, is produced and secreted by acinar cells of the rat submandibular gland. Expression of the cystatin S gene is known to be induced at high levels by the beta-adrenergic agonist isoproterenol. In the present study, we revealed that in the submandibular gland of hypophysectomized adult male rats, the levels of induced cystatin S mRNA 24 h after a single administration of isoproterenol are strikingly lower than those in the gland of normal rats. Administration of one of the pituitary-dependent hormones testosterone, estradiol, dexamethasone and thyroxine, together with isoproterenol resulted in marked enhancement of the isoproterenol-induced cystatin S mRNA expression in hypophysectomized rats, whereas administration of any of these hormones alone had no significant effect. These results suggested the existence of cross-talk between the signaling pathways of steroid hormones and isoproterenol in inducing cystatin S gene expression in the rat submandibular gland.
authors.affiliation: Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, Kanazawa 920-8640, Japan. siseki@med.kanagawa-u.ac.jp
authors.forename: Shoichi
authors.initials: S
authors.lastname: Iseki
authors.forename: Jin-Gook
authors.initials: JG
authors.lastname: Kim
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kudo
authors.forename: Yukio
authors.initials: Y
authors.lastname: Naito
authors.forename: Wiphawi
authors.initials: W
authors.lastname: Hipkaeo
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D015891:Cystatins / Q000096:biosynthesis* / Q000235:genetics; D004357:Drug Synergism; D006728:Hormones / Q000494:pharmacology; D007016:Hypophysectomy; D017403:In Situ Hybridization; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010902:Pituitary Gland / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055333:Salivary Cystatins; D015398:Signal Transduction / Q000502:physiology; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000318:Adrenergic beta-Agonists; C525174:Cyss protein, rat; D015891:Cystatins; D006728:Hormones; D012333:RNA, Messenger; D055333:Salivary Cystatins; D007545:Isoproterenol