title: Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice.
abstract: The family Acetobacteraceae currently includes three known nitrogen-fixing species, Gluconacetobacter diazotrophicus, G. johannae and G. azotocaptans. In the present study, acetic acid-producing nitrogen-fixing bacteria were isolated from four different wetland rice varieties cultivated in the state of Tamilnadu, India. Most of these isolates were identified as G. diazotrophicus on the basis of their phenotypic characteristics and PCR assays using specific primers for that species. Based on 16S rDNA partial sequence analysis and DNA: DNA reassociation experiments the remaining isolates were identified as Acetobacter peroxydans, another species of the Acetobacteraceae family, thus far never reported as diazotrophic. The presence of nifH genes in A. peroxydans was confirmed by PCR amplification with nifH specific primers. Scope for the findings: This is the first report of the occurrence and association of N2-fixing Gluconacetobacter diazotrophicus and Acetobacter peroxydans with wetland rice varieties. This is the first report of diazotrophic nature of A. peroxydans.
authors.affiliation: Main Bio-control Research Laboratory (Unit of Tamilnadu Co-operative Sugar Federation), Good Will Avenue, Venpakkam, Chengalpattu 603 111, Tamilnadu, India. mutindia@yahoo.com
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Muthukumarasamy
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: Revathi
authors.forename: Muthaiyan
authors.initials: M
authors.lastname: Vadivelu
authors.forename: D
authors.initials: D
authors.lastname: Janssens
authors.forename: B
authors.initials: B
authors.lastname: Hoste
authors.forename: Kang Ui
authors.initials: KU
authors.lastname: Gum
authors.forename: Ki-Do
authors.initials: KD
authors.lastname: Park
authors.forename: Cho Young
authors.initials: CY
authors.lastname: Son
authors.forename: Tongmin
authors.initials: T
authors.lastname: Sa
authors.forename: Jesus
authors.initials: J
authors.lastname: Caballero-Mellado
mesh_terms: D000091:Acetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D020459:Genes, rRNA; D041821:Gluconacetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007194:India; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase