Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage.
The adaptive responses to H2O2 and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H2O2 significantly protected PC12 cells against the cytotoxicity induced by lethal H2O2. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H2O2. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and paraquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H2O2 enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H2O2 and related hydroxyl radicals.
Chen
Zhi-Hua
ZH
Human Stress Signal Research Center (HSSRC), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Ikeda, Osaka 563-8577, Japan. zhihua-chen@aist.go.jp
Yoshida
Yasukazu
Y
Saito
Yoshiro
Y
Niki
Etsuo
E
D000222:Adaptation, Physiological; D018663:Adrenergic Agents / Q000633:toxicity; D000818:Animals; D002452:Cell Count / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006540:Herbicides / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016627:Oxidopamine / Q000633:toxicity; D016716:PC12 Cells / Q000187:drug effects* / Q000502:physiology; D010269:Paraquat / Q000633:toxicity; D051381:Rats; D013997:Time Factors
D018663:Adrenergic Agents; D006540:Herbicides; D016627:Oxidopamine; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D010269:Paraquat