Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies.
We have demonstrated the feasibility of detecting and quantifying six cell-cycle-related nuclear markers (Ki67, pRb, p27, phospho-p27 (phosphorylated p27), phospho-pRb (phosphorylated pRb), phospho-HH3 (phosphorylated histone H3)) in plucked human scalp and eyebrow hair. Estimates of the proportion of plucked hairs that are lost or damaged during processing plus the intra- and intersubject variability of each nuclear marker with these techniques are provided to inform sizing decisions for intervention studies with drugs potentially impacting on these markers in the future.
Camidge
D R
DR
Edinburgh Cancer Centre, Western General Hospital, Edinburgh EH4 2XU, UK.
Randall
K R
KR
Foster
J R
JR
Sadler
C J
CJ
Wright
J A
JA
Soames
A R
AR
Laud
P J
PJ
Smith
P D
PD
Hughes
A M
AM
D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation; D023381:Endpoint Determination; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013048:Specimen Handling
D000970:Antineoplastic Agents; D015415:Biomarkers