title: Quality control in combinatorial chemistry: determinations of amounts and comparison of the "purity" of LC-MS-purified samples by NMR, LC-UV and CLND.
abstract: The absolute purities of 20 purified samples from a combinatorial library have been determined by a new method that uses the DMSO sidebands [1J[13C-1H]] as an internal standard for quantification. The obtained absolute amounts are compared with the amounts of sample obtained by weighing, with the calculated weights obtained by chemiluminescent nitrogen detection (CLND) chromatography and with the relative purities obtained by LC-UV chromatography.
authors.affiliation: Novartis Institutes for BioMedical Research, Lead Synthesis & Chemogenetics and Analytical & Imaging Sciences, CH-4002 Basel, Switzerland.
authors.forename: Elodie
authors.initials: E
authors.lastname: Letot
authors.forename: Guido
authors.initials: G
authors.lastname: Koch
authors.forename: Rocco
authors.initials: R
authors.lastname: Falchetto
authors.forename: Günter
authors.initials: G
authors.lastname: Bovermann
authors.forename: Lukas
authors.initials: L
authors.lastname: Oberer
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Roth
mesh_terms: D002845:Chromatography; D002853:Chromatography, Liquid; D020650:Combinatorial Chemistry Techniques; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014894:Weights and Measures
chemical_list: D004364:Pharmaceutical Preparations; D009584:Nitrogen; D004121:Dimethyl Sulfoxide