Promoted electron transfer of mitoxantrone binding with DNA by cytochrome c.
A promoted electron transfer of an antitumor drug, mitoxantrone (MTX), intercalating into DNA duplex was successfully obtained upon addition of cytochromes c (cyt. c) in NaAc-HAc buffer solution (pH 4.5). The experimental results suggested that co-existence of MTX and cyt. c in the DNA helix is an important factor for accelerated electron transfer of MTX, where the promoter, cyt. c, operated smoothly through the DNA bridge. The UV/Vis spectroscopic experiments further confirmed the interaction process. Furthermore, a possible mechanism of such reaction was also discussed in this paper.
Li
Nan
N
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Remin Street 5625, ChangChun Jilin 130022, China.
Yang
Xiurong
X
D045304:Cytochromes c / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D004579:Electron Transport; D008942:Mitoxantrone / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D045304:Cytochromes c; D004247:DNA; D008942:Mitoxantrone