[Optimization of transfection properties of DNA-lysine dendrimer complexes].
We studied the possibility of optimizing the DNA transfection properties of carriers based on lysine dendrimers of the third and the fifth generation, including those containing a chloroacetyl or a lipophilic palmitoyl moiety at C-end. The use of lysosome-destroying antibiotic chloroquine and an amphipathic polycationic nonadecapeptide JTS-1 was found to enhance the DNA transfecting properties of the lysine dendrimers. The triple complex including DNA, a lysine dendrimer of the third generation modified with lipophylic moieties of palmitic acid at its C-end, and JTS-1 was shown to be comparable in its transfecting activity to a complex containing Escort, a commercial cationic liposome carrier.
Vlasov
G P
GP
Lesina
E A
EA
Korol'kov
V I
VI
Gur'ianov
I A
IA
Baianova
N V
NV
Baranov
A N
AN
Kisilev
A V
AV
Baranov
V S
VS
D000900:Anti-Bacterial Agents / Q000737:chemistry; D045744:Cell Line, Tumor; D002738:Chloroquine / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D010455:Peptides; D002738:Chloroquine; D004247:DNA; D001616:beta-Galactosidase; D008239:Lysine