title: Enhanced peripheral nerve regeneration through a poled bioresorbable poly(lactic-co-glycolic acid) guidance channel.
abstract: In this study we investigated the effects of materials prepared with electrical poling on neurite outgrowth in vitro and nerve regeneration in vivo. Neuro-2a cells were seeded on poled and unpoled poly(lactic-co-glycolic) (PLGA) films and observed at time periods 24, 48 and 72 h post-seeding. The percentage of cells with neurites and the neurites per cell were quantified using light microscopy. At 48 and 72 h post-seeding, both the number of cells with neurites and the neurites per cell were significantly increased on the poled films compared to those on unpoled films. An established rat sciatic nerve model was used for in vivo studies to assess the effects of PLGA guides, poled for two different periods, on peripheral nerve regeneration. Guides were inserted in rats to bridge a 1.0 cm gap created in the right sciatic nerve. After four weeks, nerves regenerated through poled guides displayed a significant increase in conduction velocity and significantly increased numbers of axons across the guides, as compared to nerves regenerating through an unpoled guidance channel. Electrical poling was shown to promote neurite growth, axon regeneration and the conduction rate of the repaired nerve. We concluded that guides prepared with electrical poling enhance peripheral nerve regeneration.
authors.affiliation: Tissue Engineering Laboratory, Robert E Wise MD Research and Education Institute, Lahey Clinic Medical Center, 31 Mall Road, Burlington, MA 01805, USA. David.J.Bryan@lahey.org
authors.forename: David J
authors.initials: DJ
authors.lastname: Bryan
authors.forename: Jin Bo
authors.initials: JB
authors.lastname: Tang
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Doherty
authors.forename: David D
authors.initials: DD
authors.lastname: Hile
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Trantolo
authors.forename: Donald L
authors.initials: DL
authors.lastname: Wise
authors.forename: Ian C
authors.initials: IC
authors.lastname: Summerhayes
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000637:transplantation; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D020426:Sciatic Neuropathy / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid