Immunoglobulin light-chain genes in the rhesus macaque I: kappa light-chain germline sequences for subgroups IGKV1, IGKV and IGKV3.
The combined processes of immunoglobulin (IG) gene rearrangement and somatic hypermutation allow for the creation of an extremely diverse antibody repertoire. Knowledge of the germline sequence of the IG genes is required so that hypermutation and the affinity matured humoral response can be properly studied. Variable region genes can be arranged into subgroups; in humans, there are 11 IGLV subgroups and six IGKV subgroups. The rhesus macaque (Macaca mulatta) is a relevant non-human primate model for human immunological systems. A number of macaque IGHV, IGHD and IGHJ genes have already been reported, but only one light-chain germline gene has been published so far. Here we report the isolation of new macaque IGKV genes by polymerase chain reaction (PCR) amplification from macaque genomic DNA using primers based on the human sequences. Twenty-eight IGKV1, 22 IGKV2 and 12 IGKV3 germline genes for the macaque were found, the open reading frames of which exhibit high homology to their human counterparts (>96, >99 and >96%, respectively).
Howard
Wendy A
WA
Department of Immunobiology, Guy's King's and St Thomas' Medical School, King's College London, Guy's Hospital, London, SE1 9RT, UK.
Bible
Jon M
JM
Finlay-Dijsselbloem
Eva
E
Openshaw
Sam
S
Dunn-Walters
Deborah K
DK
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000916:Antibody Diversity / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D066298:In Vitro Techniques; D008253:Macaca mulatta / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D027041:Somatic Hypermutation, Immunoglobulin; D013045:Species Specificity
D007145:Immunoglobulin kappa-Chains; D004247:DNA