title: Influence of environmental stresses on stability of oil-in-water emulsions containing droplets stabilized by beta-lactoglobulin-iota-carrageenan membranes.
abstract: An oil-in-water emulsion (5 wt% corn oil, 0.5 wt% beta-lactoglobulin (beta-Lg), 0.1 wt% iota-carrageenan, 5 mM phosphate buffer, pH 6.0) containing anionic droplets stabilized by interfacial membranes comprising of beta-lactoglobulin and iota-carrageenan was produced using a two-stage process. A primary emulsion containing anionic beta-Lg coated droplets was prepared by homogenizing oil and emulsifier solution together using a high-pressure valve homogenizer. A secondary emulsion containing beta-Lg-iota-carrageenan coated droplets was formed by mixing the primary emulsion with an aqueous iota-carrageenan solution. The stability of primary and secondary emulsions to sodium chloride (0-500 mM), calcium chloride (0-12 mM), and thermal processing (30-90 degrees C) were analyzed using zeta-potential, particle size and creaming stability measurements. The secondary emulsion had better stability to droplet aggregation than the primary emulsion at NaCl </=500 mM, CaCl(2) </= 2 mM, and holding temperatures </= 60 degrees C for 20 min. The interfacial engineering technology used in the study could therefore lead to the creation of food emulsions with improved stability to environmental stresses.
authors.affiliation: Biopolymer and Colloids Research Laboratory, Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA. yeunsuk@foodsci.umass.edu
authors.forename: Yeun Suk
authors.initials: YS
authors.lastname: Gu
authors.forename: L
authors.initials: L
authors.lastname: Regnier
authors.forename: D Julian
authors.initials: DJ
authors.lastname: McClements
mesh_terms: D002122:Calcium Chloride / Q000737:chemistry / Q000494:pharmacology; D002351:Carrageenan / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D008567:Membranes, Artificial; D009821:Oils / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D004655:Emulsions; D007782:Lactoglobulins; D008567:Membranes, Artificial; D009821:Oils; D014867:Water; D012965:Sodium Chloride; D002351:Carrageenan; D002122:Calcium Chloride