Effect of inclusion of serum and granulocyte-macrophage colony stimulating factor on secretion of interferon-tau during the in vitro culture of ovine embryos.
In each of three experiments, in vitro-matured and -fertilised zygotes were cultured to Day 7 post insemination in synthetic oviductal fluid (SOF). In Experiment 1, zygotes were cultured in groups in either SOF plus albumin (SOFA) or serum (SOFS) and then blastocysts were cultured individually for a further 24 h without a change of media. In Experiment 2, zygotes were cultured in groups using a 2 x 2 factorial design in SOFA or SOFS, with or without recombinant ovine granulocyte-macrophage colony stimulating factor (GM-CSF; 5 ng mL(-1)). Blastocysts were then cultured individually using a split-plot design in SOFA or SOFS with or without GM-CSF. In Experiment 3, zygotes were cultured in SOFA in which GM-CSF was absent (A) or present (P) during Days 1-3, Days 3-5 or Days 5-7 of IVC in six combinations as follows: AAA, AAP, APP, PPP, PPA and PAA. Serum or GM-CSF increased secretion of interferon (IFN)-tau in Experiments 1 and 2 both between Days 5 and 7 of group culture and during individual culture. Secretion of IFN-tau during individual culture was determined by the medium in which embryos were group cultured and the effects of GM-CSF and serum were not additive. In Experiment 3, the presence of GM-CSF between Days 1 and 3 of culture was responsible for stimulation of secretion of IFN-tau between Days 5 and 7; IFN-tau secretion was detected as early as Day 3 post insemination.
Rooke
John
J
Sustainable Livestock Systems, SAC, Craibstone Estate, Aberdeen, UK. john.rooke@sac.ac.uk
Ewen
Morag
M
McEvoy
Tom
T
Entrican
Gary
G
Ashworth
Cheryl
C
D000818:Animals; D001769:Blood; D001826:Body Fluids; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D017077:Culture Media, Conditioned; D046149:Embryo Culture Techniques / Q000662:veterinary*; D005187:Fallopian Tubes; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007370:Interferon Type I / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000378:metabolism*; D011994:Recombinant Proteins; D012709:Serum Albumin; D012756:Sheep / Q000196:embryology*; D015053:Zygote / Q000502:physiology
D003470:Culture Media; D017077:Culture Media, Conditioned; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; C020947:interferon tau; D016178:Granulocyte-Macrophage Colony-Stimulating Factor