Fabrication of an integrated PDMS microchip incorporating an LED-induced fluorescence device.
A microfluidic device with an integrated fluorescence detection system has been developed in order to miniaturize the entire analytical system. A blue or green light-emitting diode (LED) and an optical fiber were mounted in a polydimethylsiloxane-based microchip. The performance of this device was evaluated by microchip electrophoresis. When a green LED was used as the light source, the calibration curve of Sulforhodamine-101 was linear over the range 1-100 microM. The detection limit was found to be 600 nM (240 amol) for a S/N ratio of 3. When using a blue LED, the calibration curve of Fluorescein was linear over the range 0.2-100 microM. The detection limit was estimated to be 120 nM (50 amol) (S/N=3). The detection sensitivity per unit power was comparable to that of LIF. The RSD values for the migration time, peak height and peak area were 0.74, 7.18 and 9.45%, respectively. The integrated microfluidic device was successfully used to determine amino acid derivatives.
Miyaki
Kyo
K
Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
Guo
Yanli
Y
Shimosaka
Takuya
T
Nakagama
Tatsuro
T
Nakajima
Hizuru
H
Uchiyama
Katsumi
K
D000596:Amino Acids / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005336:Fiber Optic Technology; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D055100:Optical Fibers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D000596:Amino Acids; D004129:Dimethylpolysiloxanes