Gene expression profiling during the embryonic development of mouse brain using an oligonucleotide-based microarray system.
We analyzed gene expression profiles in embryonic day 12, 15, 18 and postnatal day 0 mouse brains by utilizing a GeneChip microarray. Significant differential expression was observed in 1413 of 12,422 (11.4%) represented on the chip. Then, 397 genes known to be related to neural development and functions were selected and analyzed in more detail. Clustering of the differentially expressed genes in terms of gene function and their temporal expression patterns indicated an aspect of the genetic foundation that underlies cellular events. Moreover, we identified a novel gene that encodes a putative protein kinase, Ebr kinase, which is differentially expressed in the developing brain.
Matsuki
Tohru
T
Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. matsuki@ninds.nih.gov
Hori
Gen
G
Furuichi
Teiichi
T
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D016000:Cluster Analysis; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology*; D029968:Escherichia coli Proteins; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017403:In Situ Hybridization / Q000379:methods; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D017920:Antiporters; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C075436:EmrE protein, E coli