Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage.
In the present study, we have investigated the presence of pro-opiomelanocortin C-terminal fragment derived-peptides in human articular cartilage and cultured chondrocytes. beta-Lipotropin and beta-endorphin were monitored in different cell cultures and biopsies using different techniques. Biopsies were taken from patients undergoing total knee arthroplasty due to osteoarthritis. Both fresh tissue sections and chondrocytes cultured in monolayer were used in the study. Immunohistochemistry, immunocytochemistry, reverse transcriptase-polymerase chain reaction and qualitative Western blots were carried out. The results of the reverse transcriptase-polymerase chain reaction showed transcription of a truncated-form of mRNA for pro-opiomelanocortin in native cartilage and cultured chondrocytes. There was no detection of endogenous production of beta-lipotropin or beta-endorphin in human articular chondrocytes, either in situ or in vitro. Whether pro-opiomelanocortin-derived peptides of non-cartilaginous origin are present in articular cartilage itself still remains unclear.
Andjelkov
Nenad
N
Department of Orthopedic Surgery, University Hospital of North-Norway, Tromsoe, 9038 Norway. nenad@andjelkov@fagmed.uit.no
Elvenes
Jan
J
Figenschau
Yngve
Y
Bjorkoy
Geir
G
Knutsen
Gunnar
G
Seternes
Tore
T
Johansen
Oddmund
O
D001706:Biopsy; D002358:Cartilage, Articular / Q000737:chemistry*; D002478:Cells, Cultured / Q000166:cytology; D019902:Chondrocytes / Q000737:chemistry / Q000648:ultrastructure; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D010003:Osteoarthritis / Q000235:genetics / Q000473:pathology; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001615:beta-Endorphin / Q000032:analysis*; D008083:beta-Lipotropin / Q000032:analysis*
D012333:RNA, Messenger; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D008083:beta-Lipotropin