Mechanism of the conversion of xanthine dehydrogenase to xanthine oxidase: identification of the two cysteine disulfide bonds and crystal structure of a non-convertible rat liver xanthine dehydrogenase mutant.
Mammalian xanthine dehydrogenase can be converted to xanthine oxidase by modification of cysteine residues or by proteolysis of the enzyme polypeptide chain. Here we present evidence that the Cys(535) and Cys(992) residues of rat liver enzyme are indeed involved in the rapid conversion from the dehydrogenase to the oxidase. The purified mutants C535A and/or C992R were significantly resistant to conversion by incubation with 4,4'-dithiodipyridine, whereas the recombinant wild-type enzyme converted readily to the oxidase type, indicating that these residues are responsible for the rapid conversion. The C535A/C992R mutant, however, converted very slowly during prolonged incubation with 4,4'-dithiodipyridine, and this slow conversion was blocked by the addition of NADH, suggesting that another cysteine couple located near the NAD(+) binding site is responsible for the slower conversion. On the other hand, the C535A/C992R/C1316S and C535A/C992R/C1324S mutants were completely resistant to conversion, even on prolonged incubation with 4,4'-dithiodipyridine, indicating that Cys(1316) and Cys(1324) are responsible for the slow conversion. The crystal structure of the C535A/C992R/C1324S mutant was determined in its demolybdo form, confirming its dehydrogenase conformation.
Nishino
Tomoko
T
Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. nishino@nms.ac.jp
Okamoto
Ken
K
Kawaguchi
Yuko
Y
Hori
Hiroyuki
H
Matsumura
Tomohiro
T
Eger
Bryan T
BT
Pai
Emil F
EF
Nishino
Takeshi
T
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism; D002417:Cattle; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D007313:Insecta; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009243:NAD / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011725:Pyridines / Q000737:chemistry; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D018411:Spodoptera; D013997:Time Factors; D014968:Xanthine Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D014969:Xanthine Oxidase / Q000737:chemistry* / Q000235:genetics
D004220:Disulfides; D004791:Enzyme Inhibitors; D011725:Pyridines; D011994:Recombinant Proteins; D009243:NAD; C007542:4,4'-dipyridyl disulfide; D004247:DNA; D010088:Oxidoreductases; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D003545:Cysteine; D008751:Methylene Blue