Fundamentals of modern peptide synthesis.
The purpose of this chapter is to delineate strategic considerations and provide practical procedures to enable non-experts to synthesize peptides with a reasonable chance of success. This chapter focuses on Fmoc chemistry, which is now the most commonly employed strategy for solid phase peptide synthesis (SPPS). Protocols for the synthesis of fully deprotected peptides are presented, together with a review of linkers and supports currently employed for SPPS. The principles and the different steps of SPPS (anchoring, deprotection, coupling reaction, and cleavage) are all discussed, along with their possible side reactions.
Amblard
Muriel
M
Laboratoire des Amino Acides, Peptides et Protéines-UMR-CNRS 5810, Faculté de Pharmacie, Montpellier, France.
Fehrentz
Jean-Alain
JA
Martinez
Jean
J
Subra
Gilles
G
D000596:Amino Acids / Q000737:chemistry; D005449:Fluorenes; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers; D012117:Resins, Synthetic; D012997:Solvents
D000596:Amino Acids; D005449:Fluorenes; D010455:Peptides; D011108:Polymers; D012117:Resins, Synthetic; D012997:Solvents