title: Antibacterial activity of different generation dentin-bonding systems.
abstract: OBJECTIVE
  The aim of this in vitro study was to investigate the antibacterial activities of different generation dentin-bonding systems.
  
  
  METHODS
  The antibacterial activities of dentin-bonding systems Optibond FL Primer, Single Bond, Clearfil SE Bond Primer, and Prompt-L-Pop were evaluated against Streptococcus mutans, Streptococcus salivarius, Lactobacillus casei, and Lactobacillus acidophilus. Cervitec (1% chlorhexidine varnish) was also examined as a positive control material. Disk diffusion tests with filter paper disks (n = 10) containing a drop of each material (20 microL) were used. After incubation at 37 degrees C for 48 hours, the growth inhibition zones were measured in millimeters.
  
  
  RESULTS
  Of all the materials tested, Optibond FL Primer, Clearfil SE Bond Primer, and Prompt-LPop showed larger growth inhibition zones than the control material for all bacterial strains. No antibacterial effect was noted for Single Bond. The results indicated that Optibond FL Primer had the strongest effect against the S mutans and L casei, and Clearfil SE Bond Primer produced the largest inhibition zone for S salivarius and L acidophilus among the test materials.
  
  
  CONCLUSIONS
  The antibacterial effects observed for the tested different generation dentin-bonding systems may be related to the acidic nature of the materials.
authors.affiliation: Hacettepe University, Faculty of Dentistry, Department of Conservative Dentistry, Ankara, Turkey.
authors.forename: Meserret
authors.initials: M
authors.lastname: Başeren
authors.forename: A Rüya
authors.initials: AR
authors.lastname: Yazici
authors.forename: Meral
authors.initials: M
authors.lastname: Ozalp
authors.forename: Berrin
authors.initials: B
authors.lastname: Dayangaç
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004338:Drug Combinations; D007779:Lactobacillus acidophilus / Q000187:drug effects*; D007780:Lacticaseibacillus casei / Q000187:drug effects*; D019279:Resin Cements / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013291:Streptococcus / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects*; D013943:Thymol / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; C469607:OptiBond FL; C408584:Prompt L-Pop; D019279:Resin Cements; C079161:chlorhexidine, thymol drug combination; C115612:single bond; D013943:Thymol; D017438:Bisphenol A-Glycidyl Methacrylate; D002710:Chlorhexidine