title: Expression of ubiquitin-related enzymes in the suprachiasmatic nucleus with special reference to ubiquitin carboxy-terminal hydrolase UchL1.
abstract: There is growing evidence that ubiquitin-proteasome system plays an important role for the generation of circadian rhythms in mice as in Drosophila. Here we examined the expression of ubiquitin-related enzymes (Ubce5, UbcM4, Ube2v, Ube2d2, UchL1, UchL3, Ubp41, UfdlL, beta-TrCP) in the suprachiasmatic nucleus (SCN). At mRNA level, the-expression of these enzymes were faint to moderate except ubiquitin carboxy-terminal hydrolase L1 (UchL1), a dominant deubiquitinating salvaging enzyme. Although strongly expressed in the SCN, UchL1 mRNA did not show the rhythm in the SCN in both light-dark and constant dark conditions.
authors.affiliation: Department of Brain Sciences, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yagita
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okamura
mesh_terms: D000818:Animals; D002460:Cell Line; D002940:Circadian Rhythm; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D003624:Darkness; D004330:Drosophila; D005347:Fibroblasts / Q000378:metabolism; D006867:Hydrolases / Q000096:biosynthesis*; D017403:In Situ Hybridization; D008027:Light; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D013997:Time Factors; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D025801:Ubiquitin; D012313:RNA; D006867:Hydrolases; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse; D046988:Proteasome Endopeptidase Complex