The WD-40 repeat motif of Lgl tumor suppressor proteins associated with salt tolerance and temperature sensitivity.
We have recently identified mammalian homologues of lethal giant larvae (Lgl) tumor suppressor gene, rat Rgl-1 and bovine Bgl-1, and demonstrated that they can complement yeast double mutants lacking Sop1 and Sop2, yeast homologues of Lgl. These gene products are capable of regulating cellular viability in restrictive salt and temperature environments. Since Lgl family members contain the WD-40 repeat motif, we investigated its cellular functions using mouse homologue Mgl-1 in the absence of Sop1 and Sop2 in yeasts by complementation. Interestingly, mutant forms of Mgl-1 at the conserved glycine at position 450 and aspartic acid at position 453 in the most conserved WD-40 repeat motif were not able to complement, indicating that these amino acids are critical for regulating salt tolerance and temperature sensitivity in yeast. These results shed light on the important regulation of cytoskeletal complex for cellular polarity within eukaryotic cells.
Kim
Yu-Kyung
YK
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul 135-081, Republic of Korea.
Kim
Yong-Soo
YS
Baek
Kwang-Hyun
KH
D000222:Adaptation, Physiological / Q000502:physiology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride; D013696:Temperature; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D029721:Drosophila Proteins; D025521:Tumor Suppressor Proteins; C081435:l(2)gl protein, Drosophila; D012965:Sodium Chloride