title: Antioxidative activity of volatile extracts isolated from Angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves.
abstract: Volatile extracts were isolated from dried medicinal plants [Angelica tenuissimae roots (AT, Angelica tenuissima Nakai), peppermint leaves (PL, Mentha arvensis L.), pine needles (PN, Pinus sylvestris L.), and sweet flag leaves (SF, Acorus gramineus Rhizoma)] using steam distillation under reduced pressure, followed by continuous liquid-liquid extraction (DRP-LLE). The extracts were then analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major volatile constituents of AT, PL, PN, and SF were 3-butylidene-4,5-dihydrophthalide (32 mg/g), menthol (18 mg/g), thunbergol (2.1 mg/g), and cis-asarone (37 mg/g), respectively. The inhibitory activity (%) of the extracts against hexanal oxidation ranged from 33 to 98% at a level of 50 microg/mL. Among the volatile extracts, PL and PN increased cell viabilities by 10 and 24%, respectively, at a dose of 1 microg/mL, compared to that of H2O2-treated brain neuroblastoma cells, SK-N-SH. However, a 20% reduction in the malonaldehyde level, an index for lipid peroxidation, was observed at only 1 microg/mL concentration of PN.
authors.affiliation: Department of Food Science and Technology, Dongguk University, 26, 3 Pil-Dong, Chung-gu, Seoul 100-715, South Korea.
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Ka
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Choi
authors.forename: Hang-Sook
authors.initials: HS
authors.lastname: Chun
authors.forename: Kwang-Geun
authors.initials: KG
authors.lastname: Lee
mesh_terms: D029023:Acorus / Q000737:chemistry*; D029969:Angelica / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D036142:Mentha piperita / Q000737:chemistry*; D009447:Neuroblastoma; D041605:Pinus sylvestris / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014835:Volatilization
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide