title: [Further development (MDCK) of live cold-adapted influenza vaccine: cultivation of vaccine strains in production fermenters].
abstract: Optimal conditions were developed for cultivating the cold-adapted reassortant live influenza vaccine (CARLIV) in MDCK cells, which were in their turn cultivated in fermenters with serum-free medium and microcarrier. The use of MDCK cells meets all national and WHO requirements to continuous cells used in the production of biological preparations. CARLIV cultivated under such conditions well preserve their ts-mutations and mutation, which entail substitutions of amino acids, in all CARLIV genome segments. Provided the cultivation conditions are optimal, the output of multivalent CARLIV in a 101 fermenter can reach 100000 doses.
authors.forename: Iu Z
authors.initials: IuZ
authors.lastname: Gendon
authors.forename: S G
authors.initials: SG
authors.lastname: Markushin
authors.forename: I I
authors.initials: II
authors.lastname: Akopova
authors.forename: I B
authors.initials: IB
authors.lastname: Koptiaeva
authors.forename: E A
authors.initials: EA
authors.lastname: Nechaeva
authors.forename: I A
authors.initials: IA
authors.lastname: Mazurkova
authors.forename: I F
authors.initials: IF
authors.lastname: Radaeva
authors.forename: T D
authors.initials: TD
authors.lastname: Kolokol'tsova
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D003080:Cold Temperature; D016895:Culture Media, Serum-Free; D004285:Dogs; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development*; D007252:Influenza Vaccines / Q000235:genetics / Q000592:standards*; D009154:Mutation; D016865:Reassortant Viruses / Q000235:genetics / Q000254:growth & development*; D014776:Virus Cultivation
chemical_list: D016895:Culture Media, Serum-Free; D007252:Influenza Vaccines