Impairment of NtAQP1 gene expression in tobacco plants does not affect root colonisation pattern by arbuscular mycorrhizal fungi but decreases their symbiotic efficiency under drought.
We investigated in two tobacco (Nicotiana tabacum) plant lines (wildtype or antisense mutant) whether impairment in expression of the plasma membrane aquaporin gene (NtAQP1) affects the arbuscular mycorrhizal (AM) fungal colonisation pattern or the symbiotic efficiency of AM fungi. These two objectives were investigated under well-watered and drought stress conditions. Both plant lines had a similar pattern of root colonisation under well-watered and drought stress conditions. In contrast, under drought stress, AM wildtype plants grew faster than mycorrhizal antisense plants. Plant gas exchange also appeared to depend on the expression of NtAQP1 and parallelled the determined growth increments. The implications of enhanced symplastic water transport via NtAQP1 for the efficiency of the AM symbiosis under drought stress conditions are further discussed.
Porcel
Rosa
R
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
Gómez
Manuel
M
Kaldenhoff
Ralf
R
Ruiz-Lozano
Juan Manuel
JM
D051398:Aquaporin 1 / Q000096:biosynthesis* / Q000235:genetics; D018533:Biomass; D004190:Disasters; D020868:Gene Silencing; D038821:Mycorrhizae / Q000254:growth & development*; D018517:Plant Roots / Q000382:microbiology*; D013559:Symbiosis; D014026:Nicotiana / Q000201:enzymology* / Q000382:microbiology*
D051398:Aquaporin 1