Enhancing of measles virus infection by magnetofection.
Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens.
Kadota
Shin-ichi
S
Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Kanayama
Tetsuya
T
Miyajima
Naoko
N
Takeuchi
Kaoru
K
Nagata
Kyosuke
K
D000818:Animals; D015703:Antigens, CD; D016466:CHO Cells / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism; D008280:Magnetics; D008459:Measles virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014709:Vero Cells / Q000821:virology
D015703:Antigens, CD; D006023:Glycoproteins; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D049452:Green Fluorescent Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1