Analysis of Xenopus melanotrope cell size and POMC-gene expression.
Flow cytometry was used to separate Xenopus melanotrope cells according to their size. The cells were then submitted to real-time RT-PCR to determine the level of POMC-gene expression. The results show a positive correlation between cell size and gene expression for cells from black-background (but not white-background) adapted animals.
Corstens
G J H
GJ
Department of Cellular Animal Physiology, Radboud University Nijmegen, the Netherlands.
Roubos
E W
EW
Jenks
B G
BG
Van Erp
P E J
PE
D000818:Animals; D048429:Cell Size; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics; D014982:Xenopus laevis / Q000378:metabolism*
D011333:Pro-Opiomelanocortin