title: Crystallization and preliminary X-ray crystallographic study of the viral Zalpha domain bound to left-handed Z-DNA.
abstract: The Zalpha domain (yabaZalpha(E3L)) of the E3L protein homologue from Yaba-like disease virus, a yatavirus, was co-crystallized with d(TCGCGCG)(2) in the Z-conformation. The crystals belong to the P2(1)2(1)2 space group, with unit-cell parameters a=51.20 Angstroms, b=92.45 Angstroms, c=48.02 Angstroms, alpha=beta= gamma=90 degrees. The diffraction data were collected up to a resolution of 2.2 Angstroms. The structure of viral Zalpha motif will provide an insight into how diverse Zalpha motifs recognize Z-DNA.
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Ha
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D014764:Viral Proteins / Q000737:chemistry*; D014999:Yaba monkey tumor virus / Q000737:chemistry*
chemical_list: D043542:DNA, Z-Form; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins