Cultivation conditions and properties of extracellular crude lipase from the psychrotrophic fungus Penicillium chrysogenum 9'.
Among 97 fungal strains isolated from soil collected in the arctic tundra (Spitsbergen), Penicillium chrysogenum 9' was found to be the best lipase producer. The maximum lipase activity was 68 units mL(-1) culture medium on the fifth day of incubation at pH 6.0 and 20 degrees C. Therefore, P. chrysogenum 9' was classified as a psychrotrophic microorganism. The non-specific extracellular lipase showed a maximum activity at 30 degrees C and pH 5.0 for natural oils or at pH 7.0 for synthetic substrates. Tributyrin was found to be the best substrate for lipase, among those tested. The Km and Vmax were calculated to be 2.33 mM and 22.1 units mL(-1), respectively, with tributyrin as substrate. The enzyme was inhibited more by EDTA than by phenylmethylsulfonyl fluoride and was reactivated by Ca2+. The P. chrysogenum 9' lipase was very stable in the presence of hexane and 1,4-dioxane at a concentration of 50%, whereas it was unstable in presence of xylene.
Bancerz
R
R
Department of Biochemistry, Maria Curie-Sklodovska University, Sklodovska Square 3, Lublin 20-031, Poland. rwiater@biotop.umcs.lublin.pl
Ginalska
G
G
Fiedurek
J
J
Gromada
A
A
D001110:Arctic Regions; D003902:Detergents; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D010408:Penicillium chrysogenum / Q000201:enzymology*; D012988:Soil Microbiology; D012997:Solvents; D013379:Substrate Specificity; D013696:Temperature
D003902:Detergents; D004791:Enzyme Inhibitors; D008670:Metals; D012997:Solvents; D008049:Lipase