[Cell microarrays and functional genomics].
With the complete sequencing of the human genome, research priorities have shifted from the identification of genes to the elucidation of their function. Methods currently used by scientists to characterize gene function, such as knock-out mice, are based upon loss of protein function and analysis of the resulting phenotypes to infer a potential role for the protein under scrutiny. Until now, these methods have been successful but time consuming and only a few genes at a time could be analyzed. Cell microarrays allow to simultaneously transfect thousands of different nucleic acid molecules, RNA or DNA, into adherent cells. It is then possible to analyze a large pallet of resulting phenotypes in clusters of transfected cells. We are currently manufacturing cell microarrays with collections of full-length cDNA cloned in expression vectors (gain of function analyses) or siRNA (loss of function studies) to unravel function of genes involved in differentiation and proliferation of human cells. Although there are still some technological difficulties to overcome, the potential for cell microarrays to speed up functional exploration of genomes is very promising.
Roupioz
Yoann
Y
Service de génomique fonctionnelle, CEA/DSV, Génopole d'Evry, 2, rue Gaston Crémieux, CP 22, 91057 Evry Cedex, France.
Castel
David
D
Pitaval
Amandine
A
Baghdoyan
Sandrine
S
Gidrol
Xavier
X
D002478:Cells, Cultured; D023281:Genomics / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014162:Transfection / Q000379:methods