Increased expression of ICAM-3 is associated with radiation resistance in cervical cancer.
To search for a marker that predicts the efficacy of radiation therapy in human cervical cancer, gene expression profiles between parental SiHa cervical cancer cells and radiation-resistant SiHa/R cells have been compared by the microarray technique. Microarray and Northern blot analyses demonstrated that the ICAM-3 expression was upregulated in SiHa/R cells. This increased expression of ICAM-3 in SiHa cells enhanced cell survival by about 34.3% after a 2 Gy dosage of radiation. In addition, SiHa/ICAM-3 cells showed a 2.45-fold higher level of FAK phosphorylation than that of the control cells. In tumor specimens, ICAM-3 staining was restricted to tumor stromal endothelial cells and lymphocytes. The overexpression of ICAM-3 was significantly more frequent in radiation-resistant cervical cancer specimens when compared with radiation-sensitive specimens (83.3% vs. 35.3%; p = 0.015). With these observations, we can suggest that an increased expression of ICAM-3 is associated with radiation resistance in cervical cancer cells and the expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy.
Chung
Young Min
YM
Department of Internal Medicine and Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, South Korea.
Kim
Byoung-Gie
BG
Park
Chang-Soo
CS
Huh
Seung Jae
SJ
Kim
Jhingook
J
Park
Jong Kuk
JK
Cho
Sun Mi
SM
Kim
Byung Soo
BS
Kim
Jun Suk
JS
Yoo
Young Do
YD
Bae
Duk-Soo
DS
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000166:cytology / Q000276:immunology; D003114:Colony-Forming Units Assay; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms; D008175:Lung Neoplasms; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000532:radiotherapy
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D017931:DNA Primers; C483616:ICAM3 protein, human; D034741:RNA, Small Interfering