Relationship between Porphyromonas gingivalis, Epstein-Barr virus infection and reactivation in periodontitis.
The aim of this study was to assess the relationship-between Porphyromonas gingivalis, Epstein-Barr virus (EBV) infection and reactivation in periodontitis using real-time PCR. The mean proportion of P. gingivalis cells to total bacterial cells in the saliva from EBV-positive periodontitis patients was significantly higher than that in saliva from EBV-negative patients. An EBV-positive B-cell line was used to determine whether P. gingivalis sonicate induced reactivation of EBV, using real-time PCR to measure the virus genome in the culture medium. A significant increase in EBV numbers was observed after the stimulation with P. gingivalis sonicate. These findings suggest that the interaction between EBV and P. gingivalis is bi-directional, with EBV reactivation suppressing host defenses and permitting overgrowth of P. gingivalis, and P. gingivalis having the potential to induce EBV reactivation.
Sugano
Naoyuki
N
Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan. sugano-n@dent.nihon-u.ac.jp
Ikeda
Kyoko
K
Oshikawa
Maiko
M
Idesawa
Masataka
M
Tanaka
Hajime
H
Sato
Shuichi
S
Ito
Koichi
K
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000821:virology; D002460:Cell Line / Q000821:virology; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D010518:Periodontitis / Q000209:etiology / Q000382:microbiology* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000472:pathogenicity* / Q000502:physiology; D012463:Saliva / Q000382:microbiology / Q000821:virology; D018709:Statistics, Nonparametric; D014775:Virus Activation
D017077:Culture Media, Conditioned; D004269:DNA, Bacterial; D004279:DNA, Viral