A three-dimensional atlas of pituitary gland development in the zebrafish.
The pituitary gland is unique to Chordates, with significant variation within this group, offering an excellent opportunity to increase insight into phylogenetic relationships within this phylum. The structure of the pituitary in adult Teleosts (class: Osteichthyes) is quite different from that in other chordates and is also variable among members of the class. Therefore, a complete description of the structure and development of the pituitary in members of this class is a critical component to our overall understanding of this gland. An obvious teleost model organism is the zebrafish, Danio rerio, as a significant amount of work has been done on the molecular control of pituitary development in this fish. However, very little work has been published on the morphological development of the pituitary in the zebrafish; the present study aims to fill this void. The pituitary develops from cells on the rostrodorsal portion of the head and reaches its final position, ventral to the hypothalamus, as the cephalic flexure occurs and the jaws and mouth form. The pituitary placode is juxtaposed to cells that will form the olfactory vesicles, the stomodeum, and the hatching gland. The volume of the pituitary is greatest at 24 hours post fertilization (hpf). From 24 to 120 hpf, the pituitary decreases in height and width as it undergoes convergent extension, increasing in length with the axis. The adenohypophysis is a morphologically distinct structure by 24 hpf, whereas the neurohypophysis remains indistinct until 72 hpf. The findings of this study correlate well with the available molecular data.
Chapman
Susan C
SC
Department of Neurobiology and Anatomy, and Children's Health Research Center, University of Utah School of Medicine, Salt Lake City, Utah 84132-3401, USA. susan.chapman@utah.edu
Sawitzke
Arleen L
AL
Campbell
Douglas S
DS
Schoenwolf
Gary C
GC
D000818:Animals; D000831:Animals, Newborn; D004625:Embryo, Nonmammalian; D021621:Imaging, Three-Dimensional / Q000379:methods*; D010902:Pituitary Gland / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D015027:Zebrafish