Development of a rapid HPLC method for determination of famotidine in human plasma using a monolithic column.
A rapid and sensitive HPLC method using a monolithic column has been developed for quantification of famotidine in plasma. The assay enables the measurement of famotidine for therapeutic drug monitoring with a minimum detectable limit of 5 ngml(-1). The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was carried out in reversed-phase conditions using a Chromolith Performance (RP-18e, 100 mm x 4.6 mm) column with an isocratic mobile phase consisting of 0.03 M disodium hydrogen phosphate buffer-acetonitrile (93:7, v/v) adjusted to pH 6.5. The wavelength was set at 267 nm. The calibration curve was linear over the concentration range 20-400 ngml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 8%.
Zarghi
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Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, No. 105, Shams Alley, Vali-E-Asr Ave, Across from Tavanir St., P.O. Box 14155-6153, Tehran, Iran. azarghi@yahoo.com
Shafaati
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Foroutan
S M
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Khoddam
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D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004345:Drug Industry / Q000379:methods; D015738:Famotidine / Q000032:analysis* / Q000737:chemistry; D006635:Histamine H2 Antagonists / Q000737:chemistry; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008297:Male; D010710:Phosphates / Q000737:chemistry; D011899:Ranitidine / Q000032:analysis; D012680:Sensitivity and Specificity; D013997:Time Factors
D002021:Buffers; D006635:Histamine H2 Antagonists; D010710:Phosphates; D015738:Famotidine; D006859:Hydrogen; D011899:Ranitidine