title: Persistence of rhinovirus RNA after asthma exacerbation in children.
abstract: BACKGROUND
  Rhinoviruses (RVs) are believed to cause most asthma exacerbations but their role in the severity of acute asthma and subsequent recovery of airway function is not defined. The importance of atopy in virus-host interactions is also not clear.
  
  
  OBJECTIVE
  We postulated that RV infection and atopic skin prick responses influence the severity of asthma exacerbations as measured by peak expiratory flow (PEF).
  
  
  METHODS
  Patients aged 4-12 years admitted with acute severe asthma to a hospital emergency room (ER) were recruited. PEF measurements were obtained and nasal aspirates (NA) were taken. Atopy was diagnosed by skin prick responses to allergen and the presence of RV RNA and respiratory syncytial virus (RSV) RNA in NAs was detected using validated PCR assays. Patients were restudied after 6 weeks and after 6 months.
  
  
  RESULTS
  Fifty children with acute asthma (mean age+/-SD, 7.4+/-2.7) were enrolled; atopy was present in 37 (74%). RV RNA was detected in 41 (82%) and RSV RNA in six (12%) subjects. After 6 weeks 41 patients were restudied and RV RNA was again detected in 18 (44%). RV RNA was detected after 6 months in four of 16 patients restudied (25%; P=0.008 vs. ER) and in two of nine children from a control group with stable asthma (22%; P=0.009 vs. ER). Overall PEF measurements were reduced in asthmatics admitted to ER (% predicted, 63.4+/-16.4%) but did not differ between patients with RV RNA, RSV RNA or neither virus present. In subjects with RV RNA detectable in ER and after 6 weeks, measurements of PEF in ER were significantly lower than in patients in whom RV RNA was present in ER but absent after 6 weeks (P=0.009). Regression analysis linked persistence of RV RNA, but not skin prick responses to allergen, to severity of PEF reductions in ER.
  
  
  CONCLUSIONS
  RV RNA was detectable in >40% of asthmatic children 6 weeks after an acute exacerbation. Asthma exacerbations were more severe in patients with persistence of RV RNA suggesting that the severity of acute asthma may be linked to prolonged and possibly more severe RV infections.
authors.affiliation: Department of Paediatrics and Lung Unit, University of Stellenbosch, Cape Town, South Africa.
authors.forename: S
authors.initials: S
authors.lastname: Kling
authors.forename: H
authors.initials: H
authors.lastname: Donninger
authors.forename: Z
authors.initials: Z
authors.lastname: Williams
authors.forename: J
authors.initials: J
authors.lastname: Vermeulen
authors.forename: E
authors.initials: E
authors.lastname: Weinberg
authors.forename: K
authors.initials: K
authors.lastname: Latiff
authors.forename: R
authors.initials: R
authors.lastname: Ghildyal
authors.forename: P
authors.initials: P
authors.lastname: Bardin
mesh_terms: D000208:Acute Disease; D000485:Allergens / Q000276:immunology; D001249:Asthma / Q000188:drug therapy / Q000276:immunology* / Q000503:physiopathology; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D006801:Humans; D010366:Peak Expiratory Flow Rate; D010850:Picornaviridae Infections / Q000276:immunology* / Q000503:physiopathology; D012367:RNA, Viral / Q000032:analysis* / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000503:physiopathology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012229:Rhinovirus / Q000276:immunology* / Q000302:isolation & purification; D012720:Severity of Illness Index; D012882:Skin Tests; D016896:Treatment Outcome
chemical_list: D000485:Allergens; D012367:RNA, Viral