title: [A method for the preparation of normalized cDNA libraries enriched with full-length sequences].
abstract: We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.
authors.forename: P A
authors.initials: PA
authors.lastname: Zhulidov
authors.forename: E A
authors.initials: EA
authors.lastname: Bogdanova
authors.forename: A S
authors.initials: AS
authors.lastname: Shcheglov
authors.forename: I A
authors.initials: IA
authors.lastname: Shagina
authors.forename: L L
authors.initials: LL
authors.lastname: Vagner
authors.forename: G L
authors.initials: GL
authors.lastname: Khazpekov
authors.forename: V V
authors.initials: VV
authors.lastname: Kozhemiako
authors.forename: S A
authors.initials: SA
authors.lastname: Luk'ianov
authors.forename: D A
authors.initials: DA
authors.lastname: Shagin
mesh_terms: D000818:Animals; D003386:Brachyura; D018076:DNA, Complementary / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004720:Endonucleases / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D004720:Endonucleases