Construction of novel human monoclonal antibodies neutralizing Porphyromonas gingivalis hemagglutination activity using transgenic mice expressing human Ig loci.
Porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis, and its colonization of subgingival sites is critical in the pathogenic process. One potential virulence factor, hemagglutinin, may mediate bacteria attachment onto and penetration into host cells, as well as agglutinate and lyses erythrocytes to intake heme, an absolute requirement for growth. Toward the development of passive immunotherapy, the construction of a human type monoclonal antibody, which is capable of inhibiting the hemagglutinating ability, will be significant and important. The human mAbs, both exhibiting a high degree of specificity and affinity against the recombinant 130 kDa hemagglutinin domain protein have been prepared using XenoMouse technology. The constructed Xeno-mAbs, IgG2 subclass, significantly inhibited hemagglutination of P. gingivalis and its vesicles. The newly constructed Xeno-mAbs may prove to be useful for the development of passive immunization against periodontal diseases caused by P. gingivalis infection, pending the results of fertility study in disease mode.
Shibata
Yasuko
Y
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Chiba 271-8587, Japan.
Hosogi
Yumiko
Y
Hayakawa
Mitsuo
M
Hori
Nobuaki
N
Kamada
Masafumi
M
Abiko
Yoshimitsu
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008822:Mice, Transgenic; D009500:Neutralization Tests; D010518:Periodontitis / Q000382:microbiology / Q000517:prevention & control; D016966:Porphyromonas gingivalis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D006388:Hemagglutinins