Regulation of TOP2 by various abiotic stresses including cold and salinity in pea and transgenic tobacco plants.
Although abiotic stress affects plant growth and development, demonstration of its direct effect on regulation of the components of the DNA replication machinery is largely unknown. In this work, we demonstrate that the expression of TOP2 (encoding topoisomerase II) is up-regulated by various abiotic stresses including salinity and low temperature, and phytohormones such as ABA (abscisic acid) and SA (salicylic acid). Transgenic studies with different deletion versions of the TOP2 promoter in tobacco define several promoter determinants responsible for specific abiotic stress responsiveness. Taken together, these results demonstrate a direct involvement of stress in the transcriptional regulation of TOP2.
Hettiarachchi
Gardhi H C M
GH
National Center for Plant Genome Research, JNU Campus, PO Box 10531, New Delhi, 110067, India.
Reddy
Malireddy K
MK
Sopory
Sudhir K
SK
Chattopadhyay
Sudip
S
D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D003080:Cold Temperature; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
D012333:RNA, Messenger; D012965:Sodium Chloride; D000040:Abscisic Acid; D004250:DNA Topoisomerases, Type II