Identification of residues in the hepatitis C virus core protein that are critical for capsid assembly in a cell-free system.
Significant advances have been made in understanding hepatitis C virus (HCV) replication through development of replicon systems. However, neither replicon systems nor standard cell culture systems support significant assembly of HCV capsids, leaving a large gap in our knowledge of HCV virion formation. Recently, we established a cell-free system in which over 60% of full-length HCV core protein synthesized de novo in cell extracts assembles into HCV capsids by biochemical and morphological criteria. Here we used mutational analysis to identify residues in HCV core that are important for capsid assembly in this highly reproducible cell-free system. We found that basic residues present in two clusters within the N-terminal 68 amino acids of HCV core played a critical role, while the uncharged linker domain between them was not. Furthermore, the aspartate at position 111, the region spanning amino acids 82 to 102, and three serines that are thought to be sites of phosphorylation do not appear to be critical for HCV capsid formation in this system. Mutation of prolines important for targeting of core to lipid droplets also failed to alter HCV capsid assembly in the cell-free system. In addition, wild-type HCV core did not rescue assembly-defective mutants. These data constitute the first systematic and quantitative analysis of the roles of specific residues and domains of HCV core in capsid formation.
Klein
Kevin C
KC
Department of Pathobiology, Box 357238, University of Washington, 1959 NE Pacific St., Seattle, WA 98195, USA.
Dellos
Sheri R
SR
Lingappa
Jaisri R
JR
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002213:Capsid / Q000502:physiology; D002474:Cell-Free System; D002918:Chymotrypsin; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011817:Rabbits; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D019065:Virus Assembly / Q000235:genetics* / Q000502:physiology*
D014758:Viral Core Proteins; D012694:Serine; D002918:Chymotrypsin