title: Overexpression of sphingosine kinase 1 is an oncogenic event in erythroleukemic progression.
abstract: The erythroleukemia developed by spi-1/PU.1-transgenic mice is a model of multistage oncogenic process. Isolation of tumor cells representing discrete stages of leukemic progression enables the dissection of some of the critical events required for malignant transformation. To elucidate the molecular mechanisms of multistage leukemogenesis, we developed a microarray transcriptome analysis of nontumorigenic (HS1) and tumorigenic (HS2) proerythroblasts from spi-1-transgenic mice. The data show that transcriptional up-regulation of the sphingosine kinase gene (SPHK1) is a recurrent event associated with the tumorigenic phenotype of these transgenic proerythroblasts. SPHK1 is an enzyme of the metabolism of sphingolipids, which are essential in several biologic processes, including cell proliferation and apoptosis. HS1 erythroleukemic cells engineered to overexpress the SPHK1 protein exhibited growth proliferative advantage, increased clonogenicity, and resistance to apoptosis in reduced serum level by a mechanism involving activation of the extracellular signal-related kinases 1/2 (ERK1/2) and phosphatidylinositol 3-kinase (PI3K)/AKT pathways. In addition, SPHK1-overexpressing HS1 cells acquired tumorigenicity when engrafted in vivo. Finally, enforced expression of a dominant-negative mutant of SPHK1 in HS2 tumorigenic cells or treatment with a pharmacologic inhibitor reduced both cell growth and apoptosis resistance. Altogether, these data suggest that overexpression of the sphingosine kinase may represent an oncogenic event during the multistep progression of an erythroleukemia.
authors.affiliation: Inserm U528, Institut Curie, 26, rue d'Ulm, 75248 Paris cedex 05, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Le Scolan
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pchejetski
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Banno
authors.forename: Nicole
authors.initials: N
authors.lastname: Denis
authors.forename: Patrick
authors.initials: P
authors.lastname: Mayeux
authors.forename: William
authors.initials: W
authors.lastname: Vainchenker
authors.forename: Thierry
authors.initials: T
authors.lastname: Levade
authors.forename: Françoise
authors.initials: F
authors.lastname: Moreau-Gachelin
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018450:Disease Progression; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005799:Genes, Dominant; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D000099157:Sphingosine Kinase
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); D020033:Protein Isoforms; D000099157:Sphingosine Kinase