Interaction of anticancer drug mitoxantrone with DNA analyzed by electrochemical and spectroscopic methods.
Cyclic voltammetry coupled with different spectroscopic (UV/Vis, fluorescence and Raman) techniques were used to study the interaction of mitoxantrone (MTX), an antitumor drug, with calf thymus DNA in acetate buffer solutions (pH 4.5). The interaction of MTX with DNA could result a considerable decrease in the MTX peak currents and a hypochromic and bathochromic shift in the maximum adsorption bands of MTX as well as the emission quenching in the MTX fluorescence spectra. The variations in the electrochemical and spectral characteristics of MTX indicated MTX bind to DNA by an intercalative mode. This conclusion was reinforced by Raman data. The merely particular vibrations were affected in Raman, suggesting that only a portion of the chromophore of MTX was involved in the intercalation into DNA duplex. These studies are valuable for a better understanding the detailed mode of MTX-DNA interaction, which should be important in deeper insight into the therapeutic efficacy of MTX and design of new DNA targeted drug.
Li
Nan
N
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Changchun 130022, China.
Ma
Ying
Y
Yang
Cheng
C
Guo
Liping
L
Yang
Xiurong
X
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D008942:Mitoxantrone / Q000737:chemistry*; D015394:Molecular Structure; D013053:Spectrophotometry
D000970:Antineoplastic Agents; D004247:DNA; C427203:calf thymus DNA; D008942:Mitoxantrone