Transfection of mouse macrophage metalloelastase gene into murine CT-26 colon cancer cells suppresses orthotopic tumor growth, angiogenesis and vascular endothelial growth factor expression.
A cDNA fragment coding for domains I and II of mouse macrophage metalloelastase (MME) was transfected into murine CT-26 colon cancer cells that are MME deficient. An orthotopic implantation model was established by using MME-transfected cells. In MME-transfected primary tumors, it demonstrated that tumor growth and microvessel formation were significantly inhibited compared with the controls. The expression of vascular endothelial growth factor (VEGF) mRNA and protein was significantly lower in MME-transfected group compared with those in the controls. Our data show that both MME and VEGF gene expression is highly associated with the vascularity of tumors, which may depend on a balance between MME and VEGF expression.
Shi
Hai
H
Department of Gastroenterology, the First Affiliated Hospital, Anhui Medical University, Hefei 23022, Anhui Province, China.
Xu
Jian Ming
JM
Hu
Nai Zhong
NZ
Wang
Xue Long
XL
Mei
Qiao
Q
Song
Yu Lin
YL
D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000098:blood supply / Q000473:pathology / Q000628:therapy*; D015316:Genetic Therapy; D005822:Genetic Vectors; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053508:Matrix Metalloproteinase 12; D008666:Metalloendopeptidases / Q000235:genetics* / Q000502:physiology; D051379:Mice; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
D042461:Vascular Endothelial Growth Factor A; D008666:Metalloendopeptidases; D053508:Matrix Metalloproteinase 12