Presence and active synthesis of the 67 kDa elastin-receptor in human circulating white blood cells.
Early after the identification of the elastin-receptor (El-R) on mesenchymal cells, it was demonstrated that phagocytic cells and lymphocytes could also respond to elastin peptides. Nevertheless, the level of El-R expression has never been demonstrated on immune cells and no data exist whether these cells actively synthesize this El-R. Thus, our aim in the present work was to study the expression and number of El-R on white blood cells (WBC) using a specific 67 kDa El-R antibody and to demonstrate the presence of mRNA corresponding to the gene coding for El-R. Our results show that messenger RNA corresponding to the presumptive gene coding for the 67 kDa El-R subunit could be detected in all three WBC-types investigated. On all of these WBC, the presence of El-R could be demonstrated, however their number and their function varied following the cell type. The presence of El-R is very important for the interaction of circulating cell with the matrix as these cells intervene during atherosclerosis and in host defence.
Larbi
Anis
A
Laboratoire de Bio-gérontologie, Centre de Recherche sur le vieillissement, Institut Universitaire de Gériatrie, Département de Médecine, Université de Sherbrooke, Sherbrooke, Que., Canada.
Levesque
Georges
G
Robert
Ladislas
L
Gagné
Daniéle
D
Douziech
Nadine
N
Fülöp
Tamas
T
D004549:Elastin / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D021122:Protein Subunits; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D021122:Protein Subunits; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C054901:elastin-binding proteins; D004549:Elastin