title: Guidelines for sampling, measuring and reporting ionized magnesium in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing.
abstract: All analyzers with ion-selective electrodes for ionized magnesium (iMg) should yield comparable and unbiased results. The prerequisite to achieve this goal is to reach consensus on sampling, measurement and reporting. The recommended guidelines for sampling, measurement and reporting iMg in plasma ("plasma" refers to circulating plasma and the forms in which it is sampled: the plasma phase of anticoagulated whole blood, plasma separated from blood cells, or serum) or blood, referring to the substance concentration of iMg in the calibrants, will provide results for iMg that are approximately 3% greater than its true concentration, and 4% less than its true molality. Binding of magnesium to proteins and ligands in plasma and blood is pH-dependent. Therefore, pH should be simultaneously measured to allow adjustment of iMg concentration to pH 7.4. The substance concentration of iMg may be physiologically and consequently clinically more relevant than the substance concentration of total magnesium.
authors.affiliation: Laboratory of Clinical Chemistry, National Institute of Nutrition, Bab Saadoun, Tunis, Tunisia.
authors.forename: Mohammed C Ben
authors.initials: MC
authors.lastname: Rayana
authors.forename: Robert W
authors.initials: RW
authors.lastname: Burnett
authors.forename: Arthur K
authors.initials: AK
authors.lastname: Covington
authors.forename: Paul
authors.initials: P
authors.lastname: D'Orazio
authors.forename: Niels
authors.initials: N
authors.lastname: Fogh-Andersen
authors.forename: Ellis
authors.initials: E
authors.lastname: Jacobs
authors.forename: Wolf R
authors.initials: WR
authors.lastname: Külpmann
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kuwa
authors.forename: Lasse
authors.initials: L
authors.lastname: Larsson
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewenstam
authors.forename: Anton H J
authors.initials: AH
authors.lastname: Maas
authors.forename: Gerhard
authors.initials: G
authors.lastname: Mager
authors.forename: Jerzy H J
authors.initials: JH
authors.lastname: Naskalski
authors.forename: Anthony O
authors.initials: AO
authors.lastname: Okorodudu
authors.forename: Christoph
authors.initials: C
authors.lastname: Ritter
authors.forename: Andrew
authors.initials: A
authors.lastname: St John
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D001798:Blood Proteins / Q000032:analysis; D002118:Calcium / Q000097:blood; D002413:Cations, Divalent / Q000097:blood; D004566:Electrodes; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D010949:Plasma / Q000737:chemistry; D012964:Sodium / Q000097:blood; D013501:Surface-Active Agents
chemical_list: D001798:Blood Proteins; D002413:Cations, Divalent; D013501:Surface-Active Agents; D012964:Sodium; D008274:Magnesium; D002118:Calcium