Determination of human plasma phospholipid transfer protein mass and activity.
Plasma phospholipid transfer protein (PLTP) plays an important role in lipoprotein metabolism. PLTP is an 80-kDa glycoprotein that is expressed/secreted by a wide variety of tissues including lung, liver, adipose tissue, brain, and muscle. PLTP mediates a net transfer of phospholipids between vesicles and plasma HDLs. It also generates from small HDL particles large fused HDL particles with a concomitant formation of small lipid-poor apolipoprotein (apo) A-I-containing particles which are thought to act as the primary acceptors of cell-derived cholesterol from peripheral tissue macrophages. Another important function of PLTP is connected to lipolysis. Its role in the transfer of surface remnants from triglyceride-rich particles, very-low-density lipoproteins, and chylomicrons, to HDL is of importance for the maintenance of HDL levels. Recent observations from our laboratory have demonstrated that in circulation two forms of PLTP are present, one catalytically active (high-activity form, HA-PLTP) and the other a low-activity form (LA-PLTP). In view of the likely relevancy of PLTP in human health and disease, reliable and accurate methods for measuring plasma/serum PLTP activity and concentration are required. In this chapter, two radiometric PLTP activity assays are described: (i) exogenous, lipoprotein-independent phospholipid transfer assay and (ii) endogenous, lipoprotein-dependent phospholipid transfer assay. In addition, an ELISA method for quantitation of serum/plasma total PLTP mass as well as HA-PLTP and LA-PLTP mass is reported in detail.
Jauhiainen
Matti
M
Department of Molecular Medicine, National Public Health Institute, Biomedicum, Haartmaninkatu 8, FIN-00251 Helsinki, Finland. matti.jauhiainen@ktl.fi
Ehnholm
Christian
C
D016632:Apolipoprotein A-I / Q000737:chemistry; D001774:Blood Chemical Analysis; D002784:Cholesterol / Q000378:metabolism; D002914:Chylomicrons / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D049294:Phospholipid Transfer Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D010743:Phospholipids / Q000737:chemistry; D011874:Radiometry; D014018:Tissue Distribution; D014280:Triglycerides / Q000737:chemistry
D016632:Apolipoprotein A-I; D002914:Chylomicrons; D006023:Glycoproteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008081:Liposomes; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol