title: [Effects of naoyian serum on VEGF protein expression in cultured rat cerebral microvascular endothelial cell with hypoxia].
abstract: OBJECTIVE
  To determine the effects of naoyian (NYA) serum on the expression of vascular endothelial growth factor (VEGF) protein in cultured rat cerebral microvascular endothelial cell (RCMEC) with hypoxia.
  
  
  METHODS
  NYA serum was separated from rat heart which had been filled stomach with NYA successively for 3 days. The rat cerebral microvascular endothelial cells were taken from the Sprageu-Dawley rat brain at postborn 7 days. The rat cerebral microvascular endothelial cells were incubated at anaerobic incubator to establish the hypoxia models. The vigo of RCMEC was determined by MTT. The level of expression of VEGF protein was measured by cell immunohistochemistry and Western blot.
  
  
  RESULTS
  The OD value of NYA serum group was higher than the control groups after hypoxia for 18 hours. VEGF protein was increased by hypoxia in cerebral microvascular endothelial cells (P < 0.05). The content of VEGF protein in NYA serum containing medium was more significantly elevated than those cultured in other control media (P < 0.01).
  
  
  CONCLUSIONS
  VEGF protein was induced by hypoxia in rat cerebral microvascular endothelial cells, and NYA could upregulate the expression of VEGF protein, which may be one of the protection mechanisms for cerebral microvascular endothelial cells.
authors.affiliation: Department of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South university, Changsha 410008, China.
authors.forename: Sai-ying
authors.initials: SY
authors.lastname: Wan
authors.forename: Xing-qun
authors.initials: XQ
authors.lastname: Li
authors.forename: Qin-hua
authors.initials: QH
authors.lastname: Liang
authors.forename: Yi-hui
authors.initials: YH
authors.lastname: Zhi
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Hua-xian
authors.initials: HX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002196:Capillaries / Q000166:cytology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A