Quality control in combinatorial chemistry: determinations of amounts and comparison of the "purity" of LC-MS-purified samples by NMR, LC-UV and CLND.
The absolute purities of 20 purified samples from a combinatorial library have been determined by a new method that uses the DMSO sidebands [1J[13C-1H]] as an internal standard for quantification. The obtained absolute amounts are compared with the amounts of sample obtained by weighing, with the calculated weights obtained by chemiluminescent nitrogen detection (CLND) chromatography and with the relative purities obtained by LC-UV chromatography.
Letot
Elodie
E
Novartis Institutes for BioMedical Research, Lead Synthesis & Chemogenetics and Analytical & Imaging Sciences, CH-4002 Basel, Switzerland.
Koch
Guido
G
Falchetto
Rocco
R
Bovermann
Günter
G
Oberer
Lukas
L
Roth
Hans-Jörg
HJ
D002845:Chromatography; D002853:Chromatography, Liquid; D020650:Combinatorial Chemistry Techniques; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014894:Weights and Measures
D004364:Pharmaceutical Preparations; D009584:Nitrogen; D004121:Dimethyl Sulfoxide