title: [Investigation of methicillin resistance and aminoglycoside modifying enzyme genes in hospital staphylococci by multiplex-polymerase chain reaction].
abstract: This study was conducted to investigate the presence of methicillin and aminoglycoside resistance encoding genes by multiplex-polymerase chain reaction (PCR) and by phenotypic methods in staphylococci isolated from inpatients' clinical specimens. The presence of aac(6')1aph(2"), aph(3')-IIIa and ant(4)-Ia genes encoding aminoglycoside modifying enzymes (AME) and mecA gene encoding methicillin resistance were genotypically investigated. A total of 19 S. aureus and 30 coagulase negative staphylococci (CNS) were tested. Thirty four (69.4%) of the isolates were found to be resistant to oxacillin with disk diffusion test, 33 (97%) of which were found to harbour mecA gene. The correspondance between oxacillin resistance and presence of mecA gene was found to be 100% in S. aureus isolates, while it was 95.7% in CNS. Twenty two (44.9%), 7 (14.3%) and 2 (4.1%) isolates were found to harbour aac(6')/aph(2"), aph(3')-IIIa and ant(4)-/a AME genes, respectively. At least one or more AME genes were detected in 72.7% of mecA positive isolates.
authors.affiliation: GATA Haydarpaşa Egitim Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Servisi, Istanbul.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozyurt
authors.forename: Bariş
authors.initials: B
authors.lastname: Sareyyüpoglu
authors.forename: Nurittin
authors.initials: N
authors.lastname: Ardiç
authors.forename: Ugur
authors.initials: U
authors.lastname: Ilga
authors.forename: Ali
authors.initials: A
authors.lastname: Erdemoglu
authors.forename: Tunçer
authors.initials: T
authors.lastname: Haznedaroglu
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000617:Aminoglycosides / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D003030:Coagulase; D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D019868:Kanamycin Kinase / Q000235:genetics; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009713:Nucleotidyltransferases / Q000235:genetics; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D010641:Phenotype; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000617:Aminoglycosides; D001426:Bacterial Proteins; D003030:Coagulase; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; C108453:6'-aminoglycoside acetyltransferase-2''-aminoglycoside phosphotransferase; D000123:Acetyltransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase; C428104:Ant(4')-Ia protein, Staphylococcus aureus; D009713:Nucleotidyltransferases; D010068:Oxacillin