Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup.
The recent observation of the frequent occurrence of natural recombinant Plum pox virus (PPV) isolates has led to the identification of a distinct PPV subgroup, named PPV-Rec. The diversity, origin and geographical spread of the recombinant PPV isolates belonging to this subgroup remain, however, relatively poorly known. In an effort to further our understanding of these isolates, eight PPV isolates from Serbia, the country from which the first such recombinant (PPV-o6) originated, were characterized. Depending on the genomic region targeted by different typing assays, seven of the eight isolates tested presented discrepancies in their typing behavior. Sequence analysis of the (Cter)NIb-(Nter)CP region confirmed the recombinant nature of these seven isolates which all presented an identical recombination breakpoint identical to previously characterized PPV-Rec isolates. Biological indexing and immunoblot analysis provided indications that asymptomatic infection of the GF305 peach indicator and migration of the coat protein as a double-band in immunoblots may represent conserved and discriminating properties of PPV-Rec isolates. The genetic diversity of PPV-Rec isolates from former Yugoslavia (Serbia, Bosnia and Herzegovina) was estimated to be twice as large as that of the PPV-Rec isolates obtained from all other countries to date (Albania, Bulgaria, Czech republic, Germany, Hungary and Slovakia). These last results are consistent with the hypothesis that former Yugoslavia is the center of dispersion of PPV-Rec. Taken together, the results presented here provide further evidence for the wide distribution and temporal genetic stability of these natural PPV recombinant isolates and provide for the first time a possible scenario for their dispersion throughout central and eastern Europe.
Glasa
M
M
Department of Plant Virology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia. virumig@savba.sk
Paunovic
S
S
Jevremovic
D
D
Myrta
A
A
Pittnerová
S
S
Candresse
T
T
D001483:Base Sequence; D036022:Capsid Proteins / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D005060:Europe; D005638:Fruit / Q000821:virology*; D014644:Genetic Variation; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010802:Phylogeny; D017801:Plum Pox Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D015022:Yugoslavia
D036022:Capsid Proteins; D004279:DNA, Viral