Kanamycin A-derived cationic lipids as vectors for gene transfection.
Cationic lipids nowadays constitute a promising alternative to recombinant viruses for gene transfer. We have recently explored the transfection potential of a new class of lipids based upon the use of aminoglycosides as cationic polar headgroups. The encouraging results obtained with a first cholesterol derivative of kanamycin A prompted us to investigate this family of vectors further, by modulating the constituent structural units of the cationic lipid. For this study, we have investigated the transfection properties of a series of new derivatives based on a kanamycin A scaffold. The results primarily confirm that aminoglycoside-based lipids are efficient vectors for gene transfection both in vitro and in vivo (mouse airways). Furthermore, a combination of transfection and physicochemical data revealed that some modifications of the constitutive subunits of kanamycin A-based vectors were associated with substantial changes in their transfection properties.
Sainlos
Matthieu
M
Laboratoire de Chimie des Interactions Moléculaires, Collège de France, CNRS UPR 285, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France. matthieu.sainlos@college-de-france.fr
Hauchecorne
Michelle
M
Oudrhiri
Noufissa
N
Zertal-Zidani
Samia
S
Aissaoui
Abderrahim
A
Vigneron
Jean-Pierre
JP
Lehn
Jean-Marie
JM
Lehn
Pierre
P
D000617:Aminoglycosides / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002412:Cations; D005822:Genetic Vectors; D007612:Kanamycin / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D012137:Respiratory System / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002412:Cations; D008055:Lipids; D007612:Kanamycin