Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India.
Strain Con a/3(T) is a Gram-positive, motile, endospore-forming, rod-shaped and arsenic-resistant bacterium, which was isolated from a concretion of arsenic ore obtained from a bore-hole. The bacterium grew in the presence of 20 mM arsenate and 0.5 mM arsenite. Diaminopimelic acid was present in the cell wall peptidoglycan, MK-7 was the major menaquinone, and iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0) and C(16 : 1)(delta7cis) were the major fatty acids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain Con a/3(T) was identified as a member of the genus Bacillus. It exhibited maximum similarity (97 %) at the 16S rRNA gene level with Bacillus barbaricus (DSM 14730(T)); however, the DNA-DNA relatedness value with B. barbaricus was 60 %. Strain Con a/3(T) also exhibited a number of phenotypic differences from B. barbaricus (DSM 14730(T)). Strain Con a/3(T) was therefore identified as representing a novel species of the genus Bacillus, for which the name Bacillus arsenicus sp. nov. is proposed. The type strain is Con a/3(T) (= MTCC 4380(T) = DSM 15822(T) = JCM 12167(T)).
Shivaji
S
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Suresh
K
K
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Chaturvedi
Preeti
P
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Dube
Smita
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Sengupta
S
S
Geological Survey of India, GSI Complex, DK-6, Sector-II, Bidhannagar, Kolkata - 700 091, India.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites / Q000494:pharmacology; D001407:Bacillus / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D002254:Carbonates; D003487:Cyanoacrylates / Q000032:analysis / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D003960:Diaminopimelic Acid / Q000032:analysis / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005290:Ferric Compounds; D020459:Genes, rRNA; D007194:India; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial; D024482:Vitamin K 2 / Q000032:analysis / Q000302:isolation & purification / Q000031:analogs & derivatives
D000900:Anti-Bacterial Agents; D018053:Arsenites; D002254:Carbonates; D003487:Cyanoacrylates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D005290:Ferric Compounds; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C007166:cyacrin; D003960:Diaminopimelic Acid; C062629:menaquinone 7; C486982:siderite; C015001:arsenite; D001151:Arsenic