title: A role for brain-derived neurotrophic factor in B cell development.
abstract: In the present study, we demonstrated a significant reduction of B lymphocytes in the blood, spleen and bone marrow of BDNF deficient mice. The observed developmental block in bone marrow B cell development was linked specifically to the Pre-BII stage. B lymphocytes express the BDNF receptors p75NTR and TrkB(gp95), while no BDNF expression was found. However, a strong BDNF expression was demonstrated in bone marrow stromal cells. An increase of intracellular free calcium [Ca2+]i in B lymphocytes after BDNF application confirms a direct responsiveness of B lymphocytes to BDNF. In conclusion, these results suggest a role of BDNF for normal B lymphocyte development through paracrine effects in the bone marrow.
authors.affiliation: Department of Clinical Chemistry and Molecular Diagnostics, University Hospital, Philipps-Universität, Baldingerstrasse, 35033 Marburg, Germany.
authors.forename: Berit
authors.initials: B
authors.lastname: Schuhmann
authors.forename: Alexander
authors.initials: A
authors.lastname: Dietrich
authors.forename: Serdar
authors.initials: S
authors.lastname: Sel
authors.forename: Christian
authors.initials: C
authors.lastname: Hahn
authors.forename: Martin
authors.initials: M
authors.lastname: Klingenspor
authors.forename: Marek
authors.initials: M
authors.lastname: Lommatzsch
authors.forename: Thomas
authors.initials: T
authors.lastname: Gudermann
authors.forename: Armin
authors.initials: A
authors.lastname: Braun
authors.forename: Harald
authors.initials: H
authors.lastname: Renz
authors.forename: Wolfgang Andreas
authors.initials: WA
authors.lastname: Nockher
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology / Q000473:pathology; D038041:Lymphopoiesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor