title: An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus.
abstract: A new SARS animal model was established by inoculating SARS coronavirus (SARS-CoV) into rhesus macaques (Macaca mulatta) through the nasal cavity. Pathological pulmonary changes were successively detected on days 5-60 after virus inoculation. All eight animals showed a transient fever 2-3 days after inoculation. Immunological, molecular biological, and pathological studies support the establishment of this SARS animal model. Firstly, SARS-CoV-specific IgGs were detected in the sera of macaques from 11 to 60 days after inoculation. Secondly, SARS-CoV RNA could be detected in pharyngeal swab samples using nested RT-PCR in all infected animals from 5 days after virus inoculation. Finally, histopathological changes of interstitial pneumonia were found in the lungs during the 60 days after viral inoculation: these changes were less marked at later time points, indicating that an active healing process together with resolution of an acute inflammatory response was taking place in these animals. This animal model should provide insight into the mechanisms of SARS-CoV-related pulmonary disease and greatly facilitate the development of vaccines and therapeutics against SARS.
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China, 100021.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Mingpeng
authors.initials: M
authors.lastname: She
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Marasco
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Xinming
authors.initials: X
authors.lastname: Tu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Lili
authors.initials: L
authors.lastname: Ren
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Renquan
authors.initials: R
authors.lastname: Yang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cong
authors.forename: Lan
authors.initials: L
authors.lastname: Guo
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Wang
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.forename: Yili
authors.initials: Y
authors.lastname: Sun
authors.forename: Shumin
authors.initials: S
authors.lastname: Duan
authors.forename: Jianguo
authors.initials: J
authors.lastname: Qu
authors.forename: Liangbiao
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.forename: Li
authors.initials: L
authors.lastname: Ruan
authors.forename: Peimao
authors.initials: P
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Depei
authors.initials: D
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000150:complications / Q000473:pathology; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000150:complications / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008253:Macaca mulatta; D008264:Macrophages / Q000473:pathology; D008854:Microscopy, Electron / Q000379:methods; D010614:Pharynx / Q000821:virology; D011650:Pulmonary Alveoli / Q000473:pathology; D012367:RNA, Viral / Q000032:analysis; D045473:Severe acute respiratory syndrome-related coronavirus; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000473:pathology / Q000821:virology*; D014779:Virus Replication
chemical_list: D007074:Immunoglobulin G; D012367:RNA, Viral