Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea.
Free-living nitrogen fixing bacteria were isolated from rhizosphere of seven different plant namely sesame, maize, wheat, soybean, lettuce, pepper and rice grown in Chungbuk Province, Korea. Five isolates with nitrogenase activity above 150nmol(-1) mg(-1) protein were identified based on, phenotypic and 16S rDNA sequences analysis. The strains were identified as Stenotrophomonas maltophilia (PM-1, PM-26), Bacillus fusiformis (PM-5, PM-24) and Pseudomonas fluorescens (PM-13), respectively. All the isolates produced indole-3-acetic acid (IAA), in the presence of tryptophan, ranging from 100.4 microg ml(-1) (PM-13) to 255 microg ml(-1) (PM-24). The isolate PM-24 (Bacillus fusiformis) exhibiting highest nitrogenase activity (3677.81 nmol h(-1) mg(-1) protein) and IAA production (255microg ml(-1)) has a promising potential for developing as a plant growth promoting rhizobacteria.
Park
Myoungsu
M
Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Kim
Chungwoo
C
Yang
Jinchul
J
Lee
Hyoungseok
H
Shin
Wansik
W
Kim
Seunghwan
S
Sa
Tongmin
T
D000114:Acetylene / Q000378:metabolism; D018556:Crops, Agricultural / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis; D007210:Indoleacetic Acids / Q000378:metabolism*; D007723:Korea; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D010084:Oxidation-Reduction; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; C030737:indoleacetic acid; D000114:Acetylene