title: A novel mouse model for segmental orthotopic colon cancer.
abstract: Spontaneous colon tumor mouse strains offer numerous advantages in modeling disease. However, the wide temporal window in which lesions form and the stochastic nature of lesion location require larger cohorts for assessment of disease modulation. Reliable, reproducible and inexpensive mouse models of early-stage and invasive cancer would add to existing transgenic models. We show a new method for the creation of orthotopic murine tumors centered in the mucosal and submucosal layers anywhere in the colon, allowing creation of lesions of known age, location and extent. The system overcomes the disadvantages of heterotopic implantation and allows evaluation of lesions distally in the colon as well as proximally, thereby providing an additional method to study the effects of regionality. Invasion, host vascularization and application to disparate cell lines are demonstrated. Noninvasive imaging with magnetic resonance and colonoscopy, allowed in part by the tumor location, show potential applications of this approach.
authors.affiliation: Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: Herlen
authors.initials: H
authors.lastname: Alencar
authors.forename: Ray
authors.initials: R
authors.lastname: King
authors.forename: Martin
authors.initials: M
authors.lastname: Funovics
authors.forename: Charles
authors.initials: C
authors.lastname: Stout
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.forename: Umar
authors.initials: U
authors.lastname: Mahmood
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000098:blood supply / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007413:Intestinal Mucosa / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic
chemical_list: D049452:Green Fluorescent Proteins