[Generation of dspp-LacZ transgenic mouse founders].
OBJECTIVE
To establish a transgenic mouse founders in which the expression of LacZ was directed by a dentin sialophosphoprotein-specific promoter.


METHODS
The DSPP-specific promoter was obtained by PCR and confirmed by sequencing, and the transgenic plasmid, pTN-DPM-LacZ, was constructed by subcloning the DSPP-specific promoter and LacZ-encoding sequence into one vector. The linearized transgenic plasmid was microinjected into the male pronucleus of the zygotes, and the microinjected zygotes were implanted to recipient pseudopregnant mice. The tail DNA of 4 week-pups was tested by PCR.


RESULTS
503 embryos were implanted to 20 recipient pseudopregnant mice, 12 of the 89 pups carrying the transgene. The establishment of the dspp-LacZ transgenic mouse line is under progress.


CONCLUSIONS
12 founders of the dspp-LacZ transgenic mice, in which the expression profile of LacZ should be the same as that of DSPP, were obtained by microinjection successfully, and the mouse line which is being established could be used as a good tool to investigate the exact expression profile of DSPP in the future.
Sun
Han-tang
HT
Department of Oral Medicine, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. hantang@fmmu.edu.cn
Xiao
Ming-zhen
MZ
Wu
Bu-ling
BL
Fei
Jian
J
Xu
Guo-jiang
GJ
D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D007763:Lac Operon; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein