title: An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens.
abstract: A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.
authors.affiliation: Department of Medical Microbiology, University of Aberdeen, U.K.
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Thomson
authors.forename: Hamidou
authors.initials: H
authors.lastname: Traore
authors.forename: Hasan
authors.initials: H
authors.lastname: Yesilkaya
authors.forename: Christine
authors.initials: C
authors.lastname: Doig
authors.forename: Herdis
authors.initials: H
authors.lastname: Steingrimsdottir
authors.forename: Lucia
authors.initials: L
authors.lastname: Garcia
authors.forename: Ken J
authors.initials: KJ
authors.lastname: Forbes
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D003902:Detergents; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D003902:Detergents; D011933:Reagent Kits, Diagnostic