title: Isolation of the Taenia crassiceps antigens from a phage display cDNA library and evaluation of their use for diagnosis of neurocysticercosis.
abstract: A novel cDNA cloning strategy consisting in elimination of non-coding DNA sequences from 3' regions of cDNAs was applied to construct the Taenia crassiceps phage displayed cDNA expression library. After biopanning using immune sera, three phage clones expressing T. crassiceps-derived antigens specifically recognizing antibodies present in cerebrospinal fluid and plasma samples from neuroimaging-confirmed neurocysticercosis patients were selected. This novel cloning strategy may be applied to other pathogens allowing rapid identification of peptides/proteins for immunodiagnostic tests.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, México DF CP04510, México.
authors.forename: Yetzi
authors.initials: Y
authors.lastname: Robles
authors.forename: Erik
authors.initials: E
authors.lastname: González
authors.forename: Tzipe
authors.initials: T
authors.lastname: Govezensky
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Mungia
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Acero
authors.forename: Raul J
authors.initials: RJ
authors.lastname: Bobes
authors.forename: Goar
authors.initials: G
authors.lastname: Gevorkian
authors.forename: Karen
authors.initials: K
authors.lastname: Manoutcharian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D015723:Gene Library; D006801:Humans; D051379:Mice; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D019151:Peptide Library; D013621:Taenia / Q000235:genetics* / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D019151:Peptide Library