Serological diagnosis of Trichinella spiralis in experimentally infected mice.
ELISA and IFA tests were used to detect IgG levels in sera of experimentally infected mice with Trichinella spiralis, during a period of 12 weeks post infection. A crude saline extract of muscle larvae was used for application of ELISA technique, while the intact T. spiralis muscle larvae were used as an antigen for IFAT. In both tests, T. spiralis IgG antibodies could be detected one week post infection in sera of all infected mice. ELISA test showed a sensitivity of 100% during the whole period of the experiment, while its specificity was 93.3%. Meanwhile, IFA test revealed a sensitivity of 100% and a specificity of 85%.
El-Shazly
Atef M
AM
Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Abu-Elwafa
Salah A
SA
El-Nahas
Hala A
HA
Abdel-Mageed
Aida A
AA
Soliman
Mohamed
M
El-Kadi
Mohamed A
MA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis*
D000909:Antibodies, Helminth; D007074:Immunoglobulin G