title: Large-scale imaging of cortical network activity with calcium indicators.
abstract: Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope.
authors.affiliation: Department of Biological Sciences, Columbia University, 1002 Fairchild Center, M.C. 2435 New York, NY 10027, USA. ikegaya@tk.air.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
authors.forename: Morgane
authors.initials: M
authors.lastname: Le Bon-Jego
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000818:Animals; D002118:Calcium; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium