title: Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis.
abstract: A collection of 63 isolates of Pseudomonas aeruginosa associated with ulcerative keratitis, collected from six centres in England, were typed using serotyping and random amplified polymorphic DNA-PCR, and screened for several variable virulence-related genotypes and phenotypes. Sixty-one percent of the isolates were of either serotype O1 or serotype O11, but there was no evidence for a common clone. The majority of isolates (59%) were PCR-positive for exoU rather than for exoS (38%), and carried a-type fliC genes (76%) rather than b-type (24%). Isolates were PCR-positive for pyoverdine-receptor types at a prevalence of 38% for type I, 46 % for type II and 8 % for type III. All but one of the isolates exhibited twitching activity. There was a correlation between the presence of exoS and twitching activity (P = 0.04), suggesting that a combination of exoS genotype and good twitching activity may have a role to play in ExoU-independent corneal virulence.
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: Craig
authors.initials: C
authors.lastname: Winstanley
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Kaye
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Neal
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Chilton
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: Silvia
authors.initials: S
authors.lastname: Miksch
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: C Anthony
authors.initials: CA
authors.lastname: Hart
authors.affiliation: Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.lastname: And The Microbiology Ophthalmic Group
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D003320:Corneal Ulcer / Q000382:microbiology*; D004739:England; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D010641:Phenotype; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D037521:Virulence Factors; C111749:pseudomonas exoprotein A protein, Pseudomonas aeruginosa; D036002:ADP Ribose Transferases; C444820:exoenzyme S