title: Quantitative analysis of the nephron during human fetal kidney development.
abstract: BACKGROUND
  The development of human kidney is a complex process. The number, shape, size, and distribution of nephrons as functional units in a kidney, provide some important information about the organization of the kidney. The aim of this study was to extend the knowledge of the developing human kidney by studying nephrons in the kidney's cortex during gestation.
  
  
  METHODS
  Kidney tissue specimens of 32 human fetuses, the gestational age from IV lunar month (LM IV) to LM X, were analysed. Specimens were divided in ten groups based on gestational age. Stereological methods were used at the light microscopic level to estimate the volume densities of the corpuscular and tubular components of the nephron in the cortex of the developing human kidney.
  
  
  RESULTS
  Nephron polymorphism was the main characteristic of the human fetal kidney during development. In younger fetuses, just below the renal capsule, there was a wide nephrogenic zone. It contained the condensed mesenchyme and terminal ends of the ureteric bud. Nephrons, in the different stages of development, were located around the ureteric bud which branched in the cortical nephrogenic zone and induced nephrogenesis. More mature nephrons were located in the deeper part of the cortex, close to the juxta-medullary junction. During gestation, nephrogenesis continually advanced, and the number of nephrons increased. Glomeruli changed their size and shape, while the tubules changed their length and convolution. Renal cortex became wider and contained the more mature glomeruli and the more convoluted tubules. The volume density of the tubular component of the nephron increased continually from 10.53% (LM IVa) to 27.7% (LM X). Renal corpuscles changed their volume density irregularly during gestation, increasing from 13% (LM IVa) to 15.5% (LM IVb). During the increase of gestational age, the volume density of corpuscular component of the nephron decreased to 11.7% (LM VIII), then went on increasing until the end of the intrauterine development (LM X) when corpuscles occupied 16.73% of the cortical volume. The volume density of the developing nephrons (corpuscular and tubular portion) showed the significant positive correlation (r = 0.85; p<0.01) with gestational age.
  
  
  CONCLUSIONS
  The present study was one of few quantitative studies of the human developing nephron. Knowledge about the normal development of the human kidney should be important for the future medical practice.
authors.affiliation: University of Nis, Medical Faculty, Institute of Anatomy, Clinical Centre, Nis.
authors.forename: Marija
authors.initials: M
authors.lastname: Daković-Bjelaković
authors.forename: Slobodan
authors.initials: S
authors.lastname: Vlajković
authors.forename: Rade
authors.initials: R
authors.lastname: Cukuranović
authors.forename: Svetlana
authors.initials: S
authors.lastname: Antić
authors.forename: Goran
authors.initials: G
authors.lastname: Bjelaković
authors.forename: Dejan
authors.initials: D
authors.lastname: Mitić
mesh_terms: D005260:Female; D047109:Fetal Development; D005865:Gestational Age; D006801:Humans; D008297:Male; D009399:Nephrons / Q000196:embryology*