Expression of candidate chromosome 3p21.3 tumor suppressor genes and down-regulation of BLU in some esophageal squamous cell carcinomas.
The expression of six chromosome 3p21.3 candidate tumor suppressor genes (BLU, FUS2, HYAL2, NPRL2, RASSF1A, and SEMA3B) in esophageal squamous cell carcinoma (ESCC) has been investigated. Reduced expression of BLU was detected in some ESCC cell lines and tumor tissues and the difference was quantitated by real-time quantitative polymerase chain reaction. Methylation specific-PCR revealed the down-regulation of BLU by epigenetic inactivation. However, exogenous expression of BLU did not functionally suppress tumorigenicity in nude mice. These results suggest that over-expression of BLU alone is not sufficient to inhibit tumorigenicity. Further studies on BLU interacting proteins are required to elucidate the possible role of BLU in the development of ESCC.
Yi Lo
Paulisally Hau
PH
Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (SAR), People's Republic of China.
Chung Leung
Alfred Chi
AC
Xiong
Wenjun
W
Law
Simon
S
Duh
Fuh-Mei
FM
Lerman
Michael I
MI
Stanbridge
Eric J
EJ
Lung
Maria Li
ML
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D002893:Chromosomes, Human, Pair 3; D003598:Cytoskeletal Proteins; D019175:DNA Methylation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics*; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins
D003598:Cytoskeletal Proteins; D025521:Tumor Suppressor Proteins; C472720:ZMYND10 protein, human