Substance P upregulates osteoclastogenesis by activating nuclear factor kappa B in osteoclast precursors.
CONCLUSIONS
SP upregulates osteoclastogenesis by activating NF-kappaB in osteoclast precursors.


OBJECTIVE
Osteoclastic bone resorption is a common finding of otitis media with or without cholesteatoma. Factors upregulating osteoclastogenesis are implicated in bone resorption. The aim of this study was to investigate the effect of substance P (SP) on mouse osteoclastogenesis in vitro.


METHODS
The SP dose response was analyzed in a mouse osteoclast culture induced by macrophage colony-stimulating factor and receptor activator for nuclear factor kappa B (NF-kappaB) ligand. Western blot analysis was performed to elucidate the subcellular target of SP in osteoclastogenesis.


RESULTS
Treatment with SP significantly increased osteoclastogenesis compared to control. SP activated nuclear translocation of NF-kappaB, which is an essential event in osteoclastogenesis in osteoclast precursors (bone marrow macrophages).
Sohn
Soo Joon
SJ
Department of Otolaryngology, Catholic University of Daegu School of Medicine, Daegu, South Korea. sjsohn@cu.ac.kr
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D001862:Bone Resorption / Q000503:physiopathology*; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D010033:Otitis Media / Q000503:physiopathology*; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D013373:Substance P / Q000494:pharmacology*; D051996:Transcription Factor RelA; D015854:Up-Regulation / Q000187:drug effects
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D051996:Transcription Factor RelA; D013373:Substance P; D016173:Macrophage Colony-Stimulating Factor