title: Chemopreventive effects of Paullinia cupana Mart var. sorbilis, the guaraná, on mouse hepatocarcinogenesis.
abstract: Guaraná (Paullinia cupana) is originally from Amazon, Brazil. Its effects on mouse hepatocarcinogenesis have been investigated in this study. Mice were treated with N-nitrosodiethylamine (DEN), received three different doses of P. cupana added to commercial food, and euthanized after 25 weeks. Gross lesions were quantified, and preneoplastic lesions (PNL) were histologically measured. Cellular proliferation was evaluated by immunobloting for the proliferating cell nuclear antigen (PCNA). The incidence and multiplicity of macroscopic lesions were reduced. The PNL number and PCNA expression were reduced in the highest P. cupana dose. According to these results, guaraná presented inhibitory effects on DEN hepatocarcinogenesis in mice.
authors.affiliation: Laboratory of Experimental Oncology, Department of Pathology, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr Orlando Marques de Paiva, 87. CEP 05508-900 São Paulo, SP, Brazil.
authors.forename: Heidge
authors.initials: H
authors.lastname: Fukumasu
authors.forename: Tereza Cristina
authors.initials: TC
authors.lastname: da Silva
authors.forename: José Luis
authors.initials: JL
authors.lastname: Avanzo
authors.forename: Cyntia Esteves
authors.initials: CE
authors.lastname: de Lima
authors.forename: Ivone Isabel
authors.initials: II
authors.lastname: Mackowiak
authors.forename: André
authors.initials: A
authors.lastname: Atroch
authors.forename: Helenice
authors.initials: H
authors.lastname: de Souza Spinosa
authors.forename: Fernando Salvador
authors.initials: FS
authors.lastname: Moreno
authors.forename: Maria Lucia Zaidan
authors.initials: ML
authors.lastname: Dagli
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D032126:Paullinia; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D011230:Precancerous Conditions / Q000517:prevention & control; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
chemical_list: D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D002110:Caffeine