title: Redox-responsive and calcium-dependent switching of glycosyldisulfide interactions with Concanavalin A.
abstract: Glycosyldisulfides can interact efficiently with carbohydrate-binding entities. This has been shown for a range of thiosaccharide dimers when tested for their effects against the lectin Concanavalin A using a modified quartz crystal microbalance-technique. Contrary to the thiosaccharide monomers, showing no significant binding up to 10 mM, several of the dimers showed IC(50)-values in the low millimolar range. Three of the glycosyldisulfides tested also displayed very high positive apparent cooperativity effects that were found to be both calcium-dependent and redox-responsive.
authors.affiliation: KTH-Royal Institute of Technology, Department of Chemistry, Stockholm, Sweden.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Pei
authors.forename: Teodor
authors.initials: T
authors.lastname: Aastrup
authors.forename: Henrik
authors.initials: H
authors.lastname: Anderson
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
mesh_terms: D002118:Calcium / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003208:Concanavalin A / Q000737:chemistry*; D019281:Dimerization; D006027:Glycosides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013440:Sulfides / Q000737:chemistry*
chemical_list: D006027:Glycosides; D013440:Sulfides; D003208:Concanavalin A; D002118:Calcium