Subcutaneous xenotransplantation of bovine pancreatic islets.
Transplantation of pancreatic islets in diabetes is currently limited by the need of immunosuppressive therapy. The present study was designed to test an immunoprotection planar device for subcutaneous xenotransplantation of pancreatic islets in the diabetic rat. We tested three different devices made of polyethersulfone hollow fibers. In all diabetic rats, implantation of islet-containing devices promptly normalized hyperglycemia. In vitro membrane permeability to glucose was correlated with implant function duration. These data confirm that bovine islets contained within devices and implanted subcutaneously remain functional for several days. Strategies to prolong islet function may allow achieving successful long-term islet implantation in this attractive site.
Figliuzzi
Marina
M
Department of Biomedical Engineering, Mario Negri Institute for Pharmacological Research, Via Gavazzeni 11, 24125 Bergamo, Italy. figliuzzi@marionegri.it
Cornolti
Roberta
R
Plati
Tiziana
T
Rajan
Navneeta
N
Adobati
Federica
F
Remuzzi
Giuseppe
G
Remuzzi
Andrea
A
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001705:Bioprosthesis; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D062109:Dermatologic Surgical Procedures; D003920:Diabetes Mellitus / Q000097:blood / Q000473:pathology* / Q000601:surgery*; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007515:Islets of Langerhans / Q000473:pathology*; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation* / Q000379:methods; D008297:Male; D008422:Materials Testing; D019397:Pancreas, Artificial; D011108:Polymers / Q000737:chemistry; D011474:Prosthesis Design; D051381:Rats; D017208:Rats, Wistar; D013450:Sulfones / Q000737:chemistry; D016896:Treatment Outcome
D001672:Biocompatible Materials; D001786:Blood Glucose; D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone