Sucrose-induced lupine defense against Fusarium oxysporum. Sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum.
Defense responses to inoculation with Fusarium oxysporum SCHLECHT f. sp. lupini were studied in embryo axes of Lupinus luteus L. cv. Polo cultured on a medium with sucrose (60 mM) or without it. Exogenous sucrose caused a marked endogenous increase in concentrations of sucrose, glucose and fructose in embryo axes. In axes cultured with sucrose, high performance liquid chromatography (HPLC) revealed generally higher levels of isoflavone glycosides (particularly until 48 h of culture) and free aglycones (genistein, wighteone, luteone). Inoculation resulted in a considerable decline in soluble carbohydrates between 24 and 72 h of culture. Simultaneously, the infection stimulated an increase in the level of free isoflavone aglycones in inoculated embryo axes, as compared to non-inoculated ones. Concentrations of free aglycones (i.e. genistein, wighteone and luteone) after infection were particularly high in inoculated embryo axes fed with sucrose. Genistein was a better inhibitor to F. oxysporum growth than genistein 7-O-glucoside tested. Exogenous sucrose also stimulated the activity of phenylalanine ammonialyase (PAL, EC 4.3.1.5)--an important enzyme initiating phenylpropanoid metabolism. After infection of tissues, a strong increase was observed in the activity of PAL and beta-glucosidase (EC 3.2.1.21)--an enzyme hydrolyzing isoflavone glycosides. Furthermore, the growth of inoculated embryo axes cultured with sucrose was less inhibited as a result of infection than inoculated axes cultured under carbohydrate deficiency conditions. Additionally, it had been reported previously that disease symptoms of embryo axes growing in the presence of sucrose were less intensive [30]. These results suggest that soluble sugars are involved in the mechanism of resistance, as they can stimulate phenylpropanoid metabolism and contribute to the increase in concentration of isoflavonoids, which are important elements of the defense system of legumes.
Morkunas
Iwona
I
Department of Plant Physiology, August Cieszkowski Agricultural University, Wołyńska 35, 60-637 Poznań, Poland. morkunas@jay.au.poznan.pl
Marczak
Łukasz
Ł
Stachowiak
Jerzy
J
Stobiecki
Maciej
M
D005419:Flavonoids / Q000378:metabolism*; D005670:Fusarium / Q000472:pathogenicity*; D031297:Lupinus / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism / Q000382:microbiology*; D009005:Monosaccharides / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D012995:Solubility; D013395:Sucrose / Q000494:pharmacology*; D001617:beta-Glucosidase / Q000378:metabolism
D005419:Flavonoids; D009005:Monosaccharides; D013395:Sucrose; D001617:beta-Glucosidase; D010650:Phenylalanine Ammonia-Lyase