Effects of an oxidative stress on human hemoglobin: a multiwavelength visible spectrometry study.
This study was carried out to investigate hemoglobin behavior and the role of cell membrane during oxidative stress of human red blood cells induced by a water-soluble radical initiator, 2,2'-azobis(amidino-propane) hydrochloride (AAPH) and compare the observed data to the one obtained with purified human haemoglobin solution. The different forms of hemoglobin were identified and quantified by multiwavelength visible spectrometry using multiple linear regression analysis. Hemolysis was quantified by the Drabkin method. Oxidative stress on purified hemoglobin solutions induced an early formation of Hb(+). In intact erythrocytes, no modified form of haemoglobin was found. Only the hemoglobin released by hemolysis in the extracellular medium was notified in the same way as purified haemoglobin. Thus, the cell membrane appears to protect intraerythrocytic hemoglobin toward an extracellular oxidative stress. Oxidative stress-induced by hemolysis does not seem to be due to changes in intraerythrocytic hemoglobin forms.
Lenfant
François
F
Département d'Anesthésiologie, Hôpital Général, CHU de Dijon, 3 Faubourg Raines, Dijon cedex 21033, France.
Bureau
Alain
A
Lahet
Jean-Jacques
JJ
Bouyer
Frédéric
F
Chaillot
Bernard
B
Freysz
Marc
M
D000328:Adult; D000578:Amidines / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D011188:Potassium / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*
D000578:Amidines; D006454:Hemoglobins; D016877:Oxidants; C046728:2,2'-azobis(2-amidinopropane); D011188:Potassium