title: Evaluation of cytotoxicity and antimicrobial activity of Acticoat Burn Dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice.
abstract: Cultured skin substitutes (CSS) have become a useful adjunctive treatment for closure of burn wounds, but CSS are avascular and remain susceptible to microbial destruction longer than split-thickness skin grafts. Irrigation of CSS grafted to burn wounds with a topical antimicrobial solution (TAS) has been shown to promote engraftment of CSS, but TAS usage has potential limitations. Acticoat Burn Dressing (Acticoat; Westaim Biomedical, Exeter, NH) is a silver-coated barrier dressing reported to exhibit antimicrobial activity and to reduce infection in partial-thickness and full-thickness wounds. This study evaluated the cytotoxicity of Acticoat with CSS and the efficacy of Acticoat for the management of microbial contamination in CSS grafted to full-thickness wounds in athymic mice. The cytotoxicity of Acticoat was assessed in preliminary studies after 1 week of exposure to CSS during in vitro maturation or healing on wounds in athymic mice. Histologies were analyzed and cellular viability in the CSS was determined by MTT conversion on days 0, 1, and 7 of Acticoat exposure. At 1, 2, 3, and 4 weeks after grafting, wounds were traced, and areas of healing CSS were calculated by image analysis. At 4 weeks, wound biopsies were evaluated and scored for engraftment of human cells. In a subsequent study, wounds were inoculated with strain SBI-N of Pseudomonas aeruginosa at 1 x 10(5) cfu/wound before the application of CSS or inoculated onto the surface of Acticoat. At 4 weeks, swab cultures were collected from the surface of CSS and scored for the presence of SBI-N. Statistical significance was accepted at the 95% confidence level (P <.05). The data show that exposure in vitro of CSS to Acticoat was cytotoxic within 1 day, but 1 week of exposure in vivo did not injure CSS or inhibit wound healing. Contaminated wounds treated with Acticoat healed similarly to control treatments, with comparable rates of engraftment, and detection of SBI-N on the surface of only one graft. No SBI-N was detected on CSS after inoculation onto the surface of Acticoat. These results suggest that Acticoat may be suitable as a protective dressing to reduce environmental contamination of CSS, if used in conjunction with additional antimicrobials to control organisms present in the wound.
authors.affiliation: Department of Research, Shriners Hospitals for Children, Cincinnati, Ohio 45229-3095, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Supp
authors.forename: Alice N
authors.initials: AN
authors.lastname: Neely
authors.forename: Dorothy M
authors.initials: DM
authors.lastname: Supp
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Warden
authors.forename: Steven T
authors.initials: ST
authors.lastname: Boyce
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002056:Burns / Q000382:microbiology / Q000473:pathology / Q000601:surgery*; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D011091:Polyesters; D011095:Polyethylenes; D011552:Pseudomonas Infections / Q000628:therapy; D011550:Pseudomonas aeruginosa; D012837:Silver Sulfadiazine / Q000494:pharmacology; D017768:Skin, Artificial; D014945:Wound Healing; D014946:Wound Infection / Q000517:prevention & control*
chemical_list: C117408:Acticoat; D000891:Anti-Infective Agents, Local; D011091:Polyesters; D011095:Polyethylenes; D012837:Silver Sulfadiazine