Quantitative analysis of topoisomerase IIalpha to rapidly evaluate cell proliferation in brain tumors.
Immunohistochemical cell proliferation analyses have come into wide use for evaluation of tumor malignancy. Topoisomerase IIalpha (topo IIalpha), an essential nuclear enzyme, has been known to have cell cycle coupled expression. We here show the usefulness of quantitative analysis of topo IIalpha mRNA to rapidly evaluate cell proliferation in brain tumors. A protocol to quantify topo IIalpha mRNA was developed with a real-time RT-PCR. It took only 3 h to quantify from a specimen. A total of 28 brain tumors were analyzed, and the level of topo IIalpha mRNA was significantly correlated with its immuno-staining index (p<0.0001, r=0.9077). Furthermore, it sharply detected that topo IIalpha mRNA decreased in growth-inhibited glioma cell. These results support that topo IIalpha mRNA may be a good and rapid indicator to evaluate cell proliferate potential in brain tumors.
Oda
Masashi
M
Department of Neurosurgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8315, Japan.
Arakawa
Yoshiki
Y
Kano
Hideyuki
H
Kawabata
Yasuhiro
Y
Katsuki
Takahisa
T
Shirahata
Mitsuaki
M
Ono
Makoto
M
Yamana
Norikazu
N
Hashimoto
Nobuo
N
Takahashi
Jun A
JA
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002675:Child, Preschool; D004250:DNA Topoisomerases, Type II / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D004250:DNA Topoisomerases, Type II