Highly branched stimuli responsive poly[(N-isopropyl acrylamide)-co-(1,2-propandiol-3-methacrylate)]s with protein binding functionality.
Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation. [Diagram: see text]
Carter
Steven
S
The Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry (Polymer Centre), Brook Hill, University of Sheffield, Sheffield, South Yorkshire, UK S3 7HF, United Kingdom.
Rimmer
Stephen
S
Sturdy
Alice
A
Webb
Michelle
M
D000180:Acrylic Resins / Q000378:metabolism*; D008689:Methacrylates / Q000378:metabolism*; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism*; D019946:Propylene Glycol / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000378:metabolism*
D000180:Acrylic Resins; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; C052970:poly-N-isopropylacrylamide; D019946:Propylene Glycol