Diagnosis of partially treated culture-negative bacterial meningitis using 16S rRNA universal primers and restriction endonuclease digestion.
Cerebrospinal fluid (CSF) obtained from patients with partially treated and culture-negative meningitis was subjected to PCR using 16S rDNA universal primers followed by restriction endonuclease digestion. In all, 43 patients and 7 controls were enrolled in this study. Twenty-one meningitic samples were positive by PCR. Mycobacterium tuberculosis was the causative agent in seven cases followed by Haemophilus influenzae (four), Streptococcus pneumoniae (two), Listeria monocytogenes (one), Escherichia coli (one), Pseudomonas aeruginosa (one) and Staphylococcus aureus (one). Only two meningitic CSF samples were culture-positive. In this study, PCR using bacterial 16S rDNA specific universal primers was found to be superior to conventional methods in the diagnosis of partially treated meningitis.
Pandit
Lekha
L
Department of Neurology, KS Hegde Medical Academy, Deralakatte, Mangalore, India 2Department of Fisheries Microbiology, University of Agricultural Sciences, Mangalore, India.
Kumar
Sanath
S
Department of Neurology, KS Hegde Medical Academy, Deralakatte, Mangalore, India 2Department of Fisheries Microbiology, University of Agricultural Sciences, Mangalore, India.
Karunasagar
Indrani
I
Department of Neurology, KS Hegde Medical Academy, Deralakatte, Mangalore, India 2Department of Fisheries Microbiology, University of Agricultural Sciences, Mangalore, India.
Karunasagar
Iddya
I
Department of Neurology, KS Hegde Medical Academy, Deralakatte, Mangalore, India 2Department of Fisheries Microbiology, University of Agricultural Sciences, Mangalore, India.
D017931:DNA Primers; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007194:India; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000134:cerebrospinal fluid*; D012336:RNA, Ribosomal, 16S / Q000134:cerebrospinal fluid*; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes