title: Distribution patterns of the anti-angiogenic protein ADAMTS-1 during rat development.
abstract: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-1) belongs to the large ADAM family of proteins. ADAMTS-1 contains a metalloproteinase domain, a disintegrin domain and three thrombospondin-like repeats but unlike ADAMs lacks a transmembrane domain. For the elucidation of the biological functions of ADAMTS-1, we raised new antibodies against ADAMTS-1. We show an accumulation of ADAMTS-1 protein at the basal lamina of rat embryonal epithelia of intestines, nasal cavity, choroid plexus, skin and in intracellular storage vesicles of epithelial cells. ADAMTS-1 protein seems to play a role in the development of the neuronal system, adipose tissue, muscle, heart, liver and adrenal glands. At the time of birth its presence is reduced in most organs. However, in the developing bone as well as in the skin, labelling increased towards late embryonal development. Immunoblots revealed a strong presence of a 62 kDa ADAMTS-1 fragment in kidneys, adrenal glands, lungs, intestines and heart. ADAMTS-1 was also present in the corresponding adult rat organs, but in more restricted distribution patterns. It was typically found in principal cells of collecting ducts, of the renal medulla, in ependymal cells of the ventricles and in some neurons. The results were confirmed by real-time PCR. The specific distribution pattern of ADAMTS-1 in a variety of organs during embryogenesis suggests a role of the molecule in tissue remodelling, vasculogenesis and angiogenesis.
authors.affiliation: Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
authors.forename: Willy
authors.initials: W
authors.lastname: Günther
authors.forename: Kai-Ove
authors.initials: KO
authors.lastname: Skaftnesmo
authors.forename: Hans
authors.initials: H
authors.lastname: Arnold
authors.forename: Rolf
authors.initials: R
authors.lastname: Bjerkvig
authors.forename: A Jorge A
authors.initials: AJ
authors.lastname: Terzis
mesh_terms: D051722:ADAM Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000071097:ADAMTS1 Protein; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004622:Embryo, Mammalian / Q000098:blood supply / Q000196:embryology* / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D012333:RNA, Messenger; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486324:Adamts1 protein, rat