Development of a monoclonal antibody against Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) that can detect EBNA-LP expressed in P3HR1 cells.
A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody was mapped in the W2 domain of EBNA-LP. While another monoclonal antibody to EBNA-LP, JF186, which is widely used for analyses of the viral protein, did not react with truncated forms of EBNA-LP expressed in P3HR1 cells, as reported earlier, the LP4D3 antibody did. The LP4D3 antibody will be a useful tool for further studies of EBNA-LP, especially investigations into the phenotypes of mutant EBNA-LP expressed in P3HR1 cells.
Shaku
Fumio
F
Department of Cell Regulation, Medical Research Institute, Tokyo, Japan.
Matsuda
Go
G
Furuya
Rie
R
Kamagata
Chinatsu
C
Igarashi
Mie
M
Tanaka
Michiko
M
Kanamori
Mikiko
M
Nishiyama
Yukihiro
Y
Yamamoto
Naoki
N
Kawaguchi
Yasushi
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D018604:Epitope Mapping; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C402640:EBNA-LP protein, Human herpesvirus 4; D014764:Viral Proteins