Biomaterials functionalization using a novel peptide that selectively binds to a conducting polymer.
The goal in biomaterial surface modification is to retain a material's bulk properties while modifying only its surface to possess desired recognition and specificity. Here we develop a unique strategy for surface functionalization of an electrically conductive polymer, chlorine-doped polypyrrole (PPyCl), which has been widely researched for various electronic and biomedical applications. An M13 bacteriophage library was used to screen 10(9) different 12-mer peptide inserts against PPyCl. A binding phage (phiT59) was isolated, and its binding stability and specificity to PPyCl was assessed using fluorescence microscopy and titer count analysis. The relative binding strength and mechanism of the corresponding 12-mer peptide and its variants was studied using atomic force microscopy and fluorescamine assays. Further, the T59 peptide was joined to a cell adhesive sequence and used to promote cell attachment on PPyCl. This strategy can be extended to immobilize a variety of molecules to PPyCl for numerous applications. In addition, phage display can be applied to other polymers to develop bioactive materials without altering their bulk properties.
Sanghvi
Archit B
AB
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712-1084, USA.
Miller
Kiley P-H
KP
Belcher
Angela M
AM
Schmidt
Christine E
CE
D000327:Adsorption; D017104:Bacteriophage M13 / Q000378:metabolism*; D017606:Chlorine Compounds / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods; D008422:Materials Testing / Q000379:methods*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011758:Pyrroles / Q000737:chemistry*; D013499:Surface Properties
D017606:Chlorine Compounds; D020099:Coated Materials, Biocompatible; D019151:Peptide Library; D010455:Peptides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole