2.0 A crystal structure of human ARL5-GDP3'P, a novel member of the small GTP-binding proteins.
ARL5 is a member of ARLs, which is widespread in high eukaryotes and homologous between species. But no structure or biological function of this member is reported. We expressed, purified, and resolved the structure of human ARL5 with bound GDP3'P at 2.0 A resolution. A comparison with the known structures of ARFs shows that besides the typical features of ARFs, human ARL5 has specific features of its own. Bacterially expressed human ARL5 contains bound GDP3'P which is seldom seen in other structures. The hydrophobic tail of the introduced detergent Triton X-305 binds at the possible myristoylation site of Gly2, simulating the myristoylated state of N-terminal amphipathic helix in vivo. The structural features of the nucleotide binding motifs and the switch regions prove that ARL5 will undergo the typical GDP/GTP structural cycle as other members of ARLs, which is the basis of their biological functions.
Wang
Zhan-Xin
ZX
National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, PR China.
Shi
Liang
L
Liu
Jun-Feng
JF
An
Xiao-Min
XM
Chang
Wen-Rui
WR
Liang
Dong-Cai
DC
D020727:ADP-Ribosylation Factors / Q000737:chemistry* / Q000378:metabolism; D020816:Amino Acid Motifs; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006150:Guanine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008958:Models, Molecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D055672:Static Electricity
D006150:Guanine Nucleotides; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D006153:Guanosine Diphosphate; C018957:guanosine 5'-diphosphate-3'-monophosphate; D006160:Guanosine Triphosphate; D020727:ADP-Ribosylation Factors; C475879:ARL5A protein, human