Development of a HPTLC method for in-process purity testing of pentoxifylline.
A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by-products as impurities, LiChrospher RP-18 F254s chromatoplates, acetone-chloroform-toluene-dioxane (2:2:1:1 v/v) as a mobile phase, and detection at 275 nm were employed. Linearity (r > or = 0.997), recovery (86.5-115.5%), and determination limit (0.1-0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline-containing raw materials and pharmaceuticals.
Grozdanovic
Olivera
O
Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, 11 000 Belgrade, POB 146, Serbia & Montenegro.
Antic
Dusan
D
Agbaba
Danica
D
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010431:Pentoxifylline / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D014665:Vasodilator Agents / Q000032:analysis* / Q000302:isolation & purification
D014665:Vasodilator Agents; D010431:Pentoxifylline