title: Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis.
abstract: Immunotherapeutic potential of aqueous extract of Ocimum sanctum (O. sanctum) leaf in bovine sub-clinical mastitis (SCM) was investigated. Somatic cell count (SCC), total bacterial count (TBC), milk differential leukocyte count (DLC), phagocytic activity and Phagocytic index and leukocyte lysosomal enzymes like myeloperoxidase and acid phosphatase content were evaluated after intramammary infusion of aqueous leaf extract of O. sanctum. The results revealed that the aqueous extract of O. sanctum treatment reduced the TBC and increased neutrophil and lymphocyte counts with enhanced phagocytic activity and phagocytic index. Similarly, the lysosomal enzymes contents of the milk polymorphonuclear cells (PMNs) were also enhanced significantly in animals treated with the extract. The results suggest that the crude aqueous extract of O. sanctum (leaf) possesses some biologically active principles that are antibacterial and immunomodulatory in nature. As such, the present wok substantiates the therapeutic use of medicinal herb and also emphasizes on the potential of the commonly available non-toxic substances to enhance the mammary immunity.
authors.affiliation: Division of Medicine, Indian Veterinary Research Institute, Izatnagar, UP 243 122, India. reena@ivri.up.nic.in
authors.forename: Reena
authors.initials: R
authors.lastname: Mukherjee
authors.forename: P K
authors.initials: PK
authors.lastname: Dash
authors.forename: G C
authors.initials: GC
authors.lastname: Ram
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000188:drug therapy*; D008892:Milk / Q000166:cytology; D009504:Neutrophils / Q000201:enzymology; D031339:Ocimum / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D010936:Plant Extracts; D009195:Peroxidase; D000135:Acid Phosphatase