title: Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography.
abstract: The present paper describes the development of a microemulsion electrokinetic chromatographic (MEEKC) method for simultaneous determination of andrographolide and dehydroandrographolide in traditional Chinese medicines and Chinese medicinal preparations. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent and butan-1-ol as co-solvent. The effect of temperature and pH of running buffers on separation were examined. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer, pH 9.2) allowed a useful and good reproducible separation of the studied analytes to be achieved.
authors.affiliation: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yanfang
authors.forename: Luo
authors.initials: L
authors.lastname: Xingping
authors.forename: Zhai
authors.initials: Z
authors.lastname: Zongde
authors.forename: Chen
authors.initials: C
authors.lastname: Liren
authors.forename: Li
authors.initials: L
authors.lastname: Yongmin
mesh_terms: D035685:Andrographis / Q000378:metabolism*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D000440:Butanols / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004224:Diterpenes / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012997:Solvents; D013696:Temperature; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000440:Butanols; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D004364:Pharmaceutical Preparations; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; C030419:andrographolide; C478098:dehydroandrographolide