Corynebacterium mooreparkense, a later heterotypic synonym of Corynebacterium variabile.
Strains of a Gram-positive bacterium were isolated from the Irish smear-ripened cheese Gubbeen, and assigned to a new species, Corynebacterium mooreparkense, in 2001. During a further study on the same cheese, no additional isolates from this species could be found. Instead, multiple isolates of its nearest phylogenetic neighbour, Corynebacterium variabile, were found. A first screening with rep-PCR and SDS-PAGE pointed to a similarity between C. mooreparkense and C. variabile. Following this peculiar result, attempts were made to collect all type strains deposited at different culture collections and all strains described by Brennan et al. [Int J Syst Evol Microbiol (2001) 51, 843-852]. Subsequently, 16S rRNA gene sequencing and DNA-DNA hybridizations were performed. All C. mooreparkense strains had a 16S rRNA gene sequence similarity of at least 99.5 % with C. variabile and the DNA-DNA relatedness was 95 %. On the basis of these results, it is concluded that C. mooreparkense is a later heterotypic synonym of C. variabile.
Gelsomino
Roberto
R
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
Snauwaert
Cindy
C
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
Vandemeulebroecke
Katrien
K
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
Hoste
Bart
B
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
Cogan
Timothy M
TM
Dairy Products Research Centre, Teagasc, Fermoy, Ireland.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, Gent, Belgium.
D001426:Bacterial Proteins / Q000032:analysis; D002611:Cheese / Q000382:microbiology*; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D009626:Terminology as Topic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S