title: Cooperation between alteration of DNA gyrase genes and over-expression of MexB and MexX confers high-level fluoroquinolone resistance in Pseudomonas aeruginosa strains isolated from a patient who received a liver transplant followed by treatment with fluoroquinolones.
abstract: Clinical isolates of highly fluoroquinolone-resistant Pseudomonas aeruginosa had a mutation in either A or B subunit of DNA gyrase and over-expressed MexB and MexX, the efflux system proteins. Introduction of wild-type gyrase genes of Escherichia coli into the isolates made them as fluoroquinolonesusceptible as the moderately fluoroquinolone-resistant strains that only over-expressed efflux system proteins. These findings demonstrate that high fluoroquinolone-resistance in P. aeruginosa is attributed to cooperation between alteration in DNA gyrase genes and over-expression of efflux systems proteins.
authors.affiliation: Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd, Suita, Osaka, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Niga
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ito
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oyamada
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Yamagishi
authors.forename: Manami
authors.initials: M
authors.lastname: Kadono
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishino
authors.forename: Naomasa
authors.initials: N
authors.lastname: Gotoh
authors.forename: Matsuhisa
authors.initials: M
authors.lastname: Inoue
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression; D006801:Humans; D016031:Liver Transplantation; D026901:Membrane Transport Proteins / Q000235:genetics*; D009154:Mutation; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D026901:Membrane Transport Proteins; C118597:MexB protein, Pseudomonas aeruginosa; C402791:MexXY protein, Pseudomonas aeruginosa; D027081:DNA Gyrase