title: Affinity ligands for industrial protein purification.
abstract: Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
authors.affiliation: Department of Biotechnology, Royal Institute of Technology (KTH), Albanova University Center, Stockholm, Sweden. martinl@biotech.kth.se
authors.forename: Martin
authors.initials: M
authors.lastname: Linhult
authors.forename: Susanne
authors.initials: S
authors.lastname: Gülich
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
mesh_terms: D002617:Chemical Industry / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D008024:Ligands; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D008024:Ligands; D011506:Proteins