Manufacturing of nanochannels with controlled dimensions using protease nanolithography.
The feasibility of creating nanometer scale depressions in biological substrates using active enzymes delivered with scanning probe microscopes has been previously demonstrated by us and other groups. Here we present a comprehensive study revealing the dependence of channels dimensions on the parameters of the "writing" process and provide a simple way to precisely control their dimensions. Such nanochannels may be used in nanofluidic biochip applications.
Ionescu
Rodica E
RE
Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
Marks
Robert S
RS
Gheber
Levi A
LA
D000327:Adsorption; D003460:Crystallization / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000648:ultrastructure; D008420:Manufactured Materials / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010781:Photography / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry* / Q000648:ultrastructure
D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D014357:Trypsin