Cytokine release induced by killed bacteria associated with anti-IFN-gamma antibody in Shigella infection.
An effort was made to analyze the effect of in vitro stimulation on macrophages using killed Shigella dysenteriae type-1 (KSD1) coupled with anti-Interferon Gamma (anti-IFN-gamma) antibody. The stimulated macrophages were co-cultured with primed or non-primed T-cells from Shigella infected patients. T-cell cultures were also established by co-culturing KSD1 coupled with or without PHA stimulated macrophages. Emulsified KSD1 coupled with anti-IFN-gamma antibody was found to act as a potent immunogen, inducing the release of Th1 cytokine from primed T-cells cultured in acute stage of the disease. It was observed that the levels of IFN-gamma and IL-2 production rather than IL-4 and IL-6 were increased as the disease became more severe. On comparison, the subsequent values of IL-6, IFN-gamma, IL-4 and IL-2 were found to be less significant in healthy primed T-cell cultures. This was also associated with a substantial production of superoxide ions (O2-), which probably inhibits the colonization of intracellular Shigella due to the presence of anti-IFN-gamma antibodies. On the other hand KSD1 with or without PHA failed to induce such responses. The above findings reflect that in the presence of anti-IFN-gamma antibody, KSD1 acts as a potential immunogen for eliciting cellular immunity against shigellosis.
Sinha
A K
AK
Division of Immunology and Vaccine Development (R-412), National Institute of Cholera & Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 10, India. ajoysinha@vsnl.net
Bagchi
A K
AK
D018920:Coculture Techniques; D004405:Dysentery, Bacillary / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D007378:Interleukins / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D013482:Superoxide Dismutase / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology
D007378:Interleukins; D007371:Interferon-gamma; D013482:Superoxide Dismutase