Immunoprophylaxis with DNA vaccination inhibits Th2-mediated responses and airway inflammation in mice sensitised with the major timothy grass pollen allergen Phl p 5b.
DNA vaccination is discussed as having the potential of improving therapy of type I allergies. A mouse model was used to investigate the effect of DNA vaccination against sensitisation with the clinically relevant Phl p 5b major allergen from timothy grass pollen in an immunoprophylactic experimental setup. Pre-treating the mice with Phl p 5b-DNA-construct significantly inhibited the production of specific IgE and IgG1 antibodies and prevented the development of a lung pathology associated with inflammation. Thus, immunoprophylaxis with an expression vector containing the Phl p 5b sequence totally prevented the emergence of characteristics associated with type I allergy and chronic bronchitis.
Darcan
Yasemin
Y
Division of Biochemical and Molecular Allergology, Research Centre Borstel, Parkallee 1-40, D-23845 Borstel, Germany.
Petersen
Arnd
A
Becker
Wolf-Meinhard
WM
Galle
Jürgen
J
Ernst
Martin
M
Ahmed
Jabbar
J
Seitzer
Ulrike
U
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D001982:Bronchial Diseases / Q000276:immunology / Q000517:prevention & control*; D007114:Immunization; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000008:administration & dosage* / Q000276:immunology; D011058:Pollen / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage*
D000485:Allergens; C095238:PHLPVI protein, Phleum pratense; C076049:Phl p V protein, Phleum pratense; D010940:Plant Proteins; D019444:Vaccines, DNA