title: A pre-S/S CHO-derived hepatitis B virus vaccine protects woodchucks from WHV productive infection.
abstract: We evaluated whether a non-adjuvanted vaccine derived from Chinese hamster ovary cells was capable of providing protection against woodchuck hepatitis virus (WHV). Three woodchucks were vaccinated with four 50-microg doses and challenged with a previously characterized virus isolate (WHV197). In all three animals, titre levels of antibodies against hepatitis B surface antigens (anti-HBs) exceeded 10 mIU/ml, peaking at 150 mIU/ml. Challenge resulted in productive acute infection in the two non-vaccinated woodchucks yet in none of the vaccinated woodchucks. In the vaccinated animals, there was evidence of abortive infection. The results demonstrate that a human vaccine is able to protect woodchucks from WHV infection.
authors.affiliation: Viral Hepatitis Unit, Department of Infectious, Parasitic, and Immune-Mediated Disease, Istituto Superiore di Sanità, Viale Regina Elena, 00161 Rome, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Argentini
authors.forename: Roberto
authors.initials: R
authors.lastname: Giuseppetti
authors.forename: Emilio
authors.initials: E
authors.lastname: D'Ugo
authors.forename: Valentina
authors.initials: V
authors.lastname: La Sorsa
authors.forename: Elena
authors.initials: E
authors.lastname: Tritarelli
authors.forename: Sara
authors.initials: S
authors.lastname: Orobello
authors.forename: Andrea
authors.initials: A
authors.lastname: Canitano
authors.forename: Reinhard
authors.initials: R
authors.lastname: Glück
authors.forename: Maria
authors.initials: M
authors.lastname: Rapicetta
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000821:virology; D008392:Marmota; D016133:Polymerase Chain Reaction; D014766:Viremia
chemical_list: D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines