Cell separation between mesenchymal progenitor cells through porous polymeric membranes.
This study investigates the separation of two types of marrow stromal cells, KUSA-A1 osteoblasts and H-1/A preadipocytes, by filtration through various porous polymeric membranes. It was found that KUSA-A1 permeates better than H-1/A cells through 12-microm polyurethane foaming membranes. This appears to be due to the relatively smaller cell size of KUSA-A1 cells. In addition, when feed solutions containing suspensions of either cell type or a mixture of the two were used, the permeation ratio was relatively low (< 6%) through polyurethane and surface-modified polyurethane foaming membranes. It was also found that there was some degree of separation between KUSA-A1 and H-1/A cells (separation factor = 1.8) with nylon-net filter membranes, but no separation was obtained when filters made of nonwoven fabrics or silk screens were used. This ability of the nylon-net filter membranes to separate the two cell types was due to a sieving effect that results from an optimal pore size. Finally, permeation of a solution of human serum albumin through the membrane following filtration of the cells did not result in a separation of cells in the recovery solution.
Higuchi
Akon
A
Department of Applied Chemistry, Seikei University, Musashino, Tokyo, Japan.
Shindo
Yosuke
Y
Gomei
Yumiko
Y
Mori
Taisuke
T
Uyama
Taro
T
Umezawa
Akihiro
A
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D002462:Cell Membrane; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D005434:Flow Cytometry; D008567:Membranes, Artificial; D008648:Mesoderm / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D010006:Osteoblasts / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D012542:Scattering, Radiation; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D017154:Stromal Cells / Q000166:cytology; D013499:Surface Properties
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D011140:Polyurethanes