title: Endothelial nitric oxide synthase upregulation in the guinea pig organ of Corti after acute noise trauma.
abstract: Endothelial nitric oxide synthase (eNOS) upregulation was identified 60 h after acute noise trauma in morphologically intact cells of the reticular lamina in the organ of Corti of the guinea pig in the second turn of the cochlea. Using gold-coupled anti-eNOS antibodies and electron microscopy, it was shown that eNOS expression was upregulated in all cell areas and cell types except inner hair cells. Furthermore, eNOS was found in the organelle-free cytoplasm and in mitochondria of various cell types. The density of eNOS in mitochondria was considerably higher compared with the surrounding cytoplasm. Since eNOS activity is regulated by calcium, the eNOS detection was combined with calcium precipitation, a method for visualizing intracellular Ca2+ distribution. After acute noise trauma, intracellular Ca2+ was increased in all cell types and cell areas except in outer hair cells. Comparing the distribution patterns of eNOS and calcium, significantly elevated levels (P < 0.0001) of eNOS were detected within a 100 nm radius near calcium precipitates in all cuticular structures as well as microtubule-rich regions and Deiters' cells near Hensen cells. The observed colocalization lends support to the postulated mechanism of eNOS activation by Ca2+. eNOS upregulation after acute noise trauma might therefore be part of an induced stress response. The eNOS upregulation in cell areas with numerous microtubule- and actin-rich structures is discussed with respect to possible cytoskeleton-dependent processes in eNOS regulation.
authors.affiliation: Department of Otolaryngology--Head and Neck Surgery, Johannes Gutenberg University Medical School, 55131 Mainz, Germany. Heinrich@hno.klinik.uni-mainz.de
authors.forename: Ulf-Rüdiger
authors.initials: UR
authors.lastname: Heinrich
authors.forename: Oxana
authors.initials: O
authors.lastname: Selivanova
authors.forename: Ralph
authors.initials: R
authors.lastname: Feltens
authors.forename: Jürgen
authors.initials: J
authors.lastname: Brieger
authors.forename: Wolf
authors.initials: W
authors.lastname: Mann
mesh_terms: D000161:Acoustic Stimulation; D008841:Actin Cytoskeleton / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology; D003593:Cytoplasm / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D003599:Cytoskeleton / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D004195:Disease Models, Animal; D004331:Drosophila melanogaster; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D006317:Hearing Loss, Noise-Induced / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008928:Mitochondria / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D009622:Noise / Q000009:adverse effects*; D009925:Organ of Corti / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D013312:Stress, Physiological / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D002118:Calcium