title: [Os(bpy)2(CO)(enIA)][OTf]2: a novel sulfhydryl-specific metal-ligand complex.
abstract: The synthesis and physical-chemical characterization of the metal-ligand complex [Os(bpy)2(CO)(enIA)][OTf]2 (where enIA = ethylenediamine iodoacetamide) with a sulfhydryl-specific functional group is described. The UV and visible absorption and luminescence emission, including lifetime and steady-state anisotropy, are reported for the free probe and the probe covalently linked to two test proteins. The spectroscopic properties of the probe are unaffected by chemical modification and subsequent covalent linkage to the proteins. The luminescence lifetime in aqueous buffer is approximately 200 ns and the limiting anisotropy is greater than 0.125, suggesting a potentially useful probe for biophysical investigations.
authors.affiliation: Dipartimento di Chimica IFM, Università di Torino, Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Garino
authors.forename: Simona
authors.initials: S
authors.lastname: Ghiani
authors.forename: Roberto
authors.initials: R
authors.lastname: Gobetto
authors.forename: Carlo
authors.initials: C
authors.lastname: Nervi
authors.forename: Luca
authors.initials: L
authors.lastname: Salassa
authors.forename: Valentina
authors.initials: V
authors.lastname: Ancarani
authors.forename: Paolo
authors.initials: P
authors.lastname: Neyroz
authors.forename: Laurie
authors.initials: L
authors.lastname: Franklin
authors.forename: J B
authors.initials: JB
authors.lastname: Alexander Ross
authors.forename: Eleanore
authors.initials: E
authors.lastname: Seibert
mesh_terms: D003545:Cysteine / Q000737:chemistry; D045647:DNA Glycosylases / Q000737:chemistry; D004563:Electrochemistry; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D008024:Ligands; D049449:Luminescence; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009992:Osmium / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C501372:(Os(bpy)2(CO)(enIA))(OTf)2; D008024:Ligands; D009942:Organometallic Compounds; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D009992:Osmium; D045647:DNA Glycosylases; D003545:Cysteine