title: Isolation and purification of coumarin compounds from Cortex fraxinus by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of coumarin compounds from Cortex fraxinus, the Chinese herbal drug. n-Butanol-methanol-0.5% acetic acid (5:1.5:5, v/v) was used as the two-phase solvent system. 14.3 mg of fraxin, 26.5 mg of aesculin, 5.8 mg of fraxetin and 32.4 mg of aesculetin with the purity of 97.6, 99.5, 97.2 and 98.7%, respectively were obtained from 150 mg of crude extracts of C. fraxinus in a single run. The structures of the isolated compounds were identified by 1H NMR and 13C NMR.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Sun
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.forename: Jichun
authors.initials: J
authors.lastname: Cui
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal