Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus.
A Gram-positive, spore-forming and moderately halophilic bacterium, strain BH139(T), was isolated from saline sediment of Xinjiang Province, China. Cells of strain BH139(T) were motile, flagellated rods. The organism grew optimally at 30-35 degrees C in the presence 12-14 % (w/v) NaCl. The major fatty acids were branched saturated fatty acids such as iso-C(16 : 0), anteiso-C(15 : 0), iso-C(15 : 0), iso-C(14 : 0) and anteiso-C(17 : 0). The G + C content of the genomic DNA was about 43 mol% and the predominant isoprenoid quinone was MK-7. Comparative 16S rRNA gene sequence analyses showed that strain BH139(T) was most closely related to Lentibacillus salicampi KCCM 41560(T) (96.9 % 16S rRNA gene sequence similarity) and formed a distinct phyletic line from that species. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus salarius sp. nov. is proposed. The type strain is BH139(T) (= KCTC 3911(T) = DSM 16459(T)).
Jeon
Che Ok
CO
Environmental Biotechnology National Core Research Center, Division of Environmental Biotechnology, Gyeongsang National University, 660-701, Korea.
Lim
Jee-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Gye Suk
GS
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jung-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Xu
Li-Hua
LH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Jiang
Cheng-Lin
CL
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
D001406:Bacillaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005407:Flagella / Q000502:physiology; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005840:Gentian Violet; D019015:Geologic Sediments / Q000382:microbiology*; D006131:Growth Inhibitors / Q000494:pharmacology; D008969:Molecular Sequence Data; D009068:Movement; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D012988:Soil Microbiology; D013171:Spores, Bacterial; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D006131:Growth Inhibitors; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet