title: Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum.
abstract: Two sporulating bacterial strains designated CECAP06(T) and CECAP16 were isolated from the rhizosphere of the legume Cicer arietinum in Argentina. Almost-complete 16S rRNA gene sequences identified the isolates as a Paenibacillus species. It was most closely related to Paenibacillus cineris LMG 18439(T) (99.6 % sequence similarity), Paenibacillus favisporus LMG 20987(T) (99.4 % sequence similarity) and Paenibacillus azoreducens DSM 13822(T) (97.7 % sequence similarity). The cells of this novel species were motile, sporulating, rod-shaped, Gram-positive and strictly aerobic. The predominant fatty acids were anteiso-C(15 : 0), C(16 : 0) and iso-C(16 : 0). The DNA G+C content of strains CECAP06(T) and CECAP16 was 51.3 and 50.9 mol%, respectively. Growth was observed from many carbohydrates, but gas production was not observed from glucose. Catalase and oxidase activities were present. The isolates produced beta-galactosidase and hydrolysed aesculin. Gelatinase, caseinase and urease were not produced. The results of DNA-DNA hybridization showed that the strains from this study constitute a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizosphaerae sp. nov. is proposed. The type strain is CECAP06(T) (=LMG 21955(T) = CECT 5831(T)).
authors.affiliation: Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus M. Unamuno, 37007 Salamanca, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rivas
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Gutiérrez
authors.affiliation: Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Córdoba, Argentina.
authors.forename: Adriana
authors.initials: A
authors.lastname: Abril
authors.affiliation: Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus M. Unamuno, 37007 Salamanca, Spain.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Mateos
authors.affiliation: Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus M. Unamuno, 37007 Salamanca, Spain.
authors.forename: Eustoquio
authors.initials: E
authors.lastname: Martínez-Molina
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
authors.affiliation: Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus M. Unamuno, 37007 Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
mesh_terms: D000332:Aerobiosis; D001118:Argentina; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D029863:Cicer / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004929:Esculin / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010619:Phenazines; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial; D001617:beta-Glucosidase / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004929:Esculin; D010088:Oxidoreductases; D002374:Catalase; D001617:beta-Glucosidase; D005840:Gentian Violet