title: Proinflammatory cytokines induce lymphocyte apoptosis in acute African swine fever infection.
abstract: Twenty-one pigs inoculated with a highly virulent isolate (E70) of African swine fever (ASF) virus were killed 1-7 days later; a further three animals served as uninfected controls. An early increase in TNF-alpha, IL-1alpha, IL-1beta and IL-6 expression was detected in lymphoid organs from infected animals, together with an increase in the serum concentrations of TNF-alpha and IL-1beta. These changes were accompanied by increased apoptosis of lymphocytes, and the presence of infected and uninfected macrophages showing changes indicative of secretory and phagocytic activation. The present study demonstrated an increase in the number of macrophages expressing TNF-alpha, IL-1 and IL-6 in proximity to lymphocytes undergoing apoptosis, supporting previous suggestions that in acute ASF proinflammatory cytokines induce lymphocyte apoptosis.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Edificio de Sanidad Animal, Campus Universitario de Rabanales, Universidad de Córdoba, 14014 Córdoba, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: P J
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: A
authors.initials: A
authors.lastname: Núñez
authors.forename: M
authors.initials: M
authors.lastname: Fernández de Marco
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
mesh_terms: D000357:African Swine Fever / Q000276:immunology* / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D025701:Asfarviridae / Q000472:pathogenicity* / Q000502:physiology; D002452:Cell Count; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D020287:In Situ Nick-End Labeling; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008221:Lymphoid Tissue / Q000276:immunology* / Q000821:virology; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure / Q000821:virology; D008297:Male; D013154:Spleen / Q000276:immunology / Q000821:virology; D013552:Swine; D013950:Thymus Gland / Q000276:immunology / Q000821:virology
chemical_list: D016207:Cytokines