title: Differential cytotoxic effects of Annona squamosa seed extracts on human tumour cell lines: role of reactive oxygen species and glutathione.
abstract: Annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. In this study, organic and aqueous extracts from the defatted seeds of Annona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. While organic and aqueous extracts induced apoptosis in MCF-7 and K-562 cells, they failed to do so in COLO-205 cells. Treatment of MCF-7 and K-562 cells with organic and aqueous extracts resulted in nuclear condensation, DNA fragmentation, induction of reactive oxygen species (ROS) generation and reduced intracellular glutathione levels. In addition downregulation of Bcl-2 and PS externalization by Annexin-V staining suggested induction of apoptosis in MCF-7 and K-562 cells by both the extracts through oxidative stress. On the contrary, COLO-205 cells showed only PS externalization but no change in ROS and glutathione levels. These observations suggest that the induction of apoptosis by A. squamosa extracts can be selective for certain types of cancerous cells.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: B V V
authors.initials: BV
authors.lastname: Pardhasaradhi
authors.forename: Madhurima
authors.initials: M
authors.lastname: Reddy
authors.forename: A Mubarak
authors.initials: AM
authors.lastname: Ali
authors.forename: A Leela
authors.initials: AL
authors.lastname: Kumari
authors.forename: Ashok
authors.initials: A
authors.lastname: Khar
mesh_terms: D054378:Acetogenins; D017304:Annexin A5; D031041:Annona / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D005233:Fatty Alcohols / Q000378:metabolism / Q000633:toxicity*; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D007783:Lactones / Q000378:metabolism / Q000633:toxicity*; D010936:Plant Extracts / Q000378:metabolism / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry*
chemical_list: D054378:Acetogenins; D017304:Annexin A5; D005233:Fatty Alcohols; D007783:Lactones; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D005978:Glutathione