The transcriptional response to hypoxic insult controlled by FRA-2.
FRA-2 is involved in cellular differentiation and is also upregulated in response to ischemic injury to the brain. To shed light on the function of this transcription factor, a novel microarray analysis was utilized to identify FRA-2-dependent gene expression increased in the hypoxic response. Genes were identified that were upregulated by exposure of neuronally differentiated PC12 cells to hypoxia. Using a dominant negative construct to block FRA-2, a second subset of genes that were FRA-2 dependent was found. Cross comparison then allowed isolation of a list of genes that were induced in response to hypoxia in a FRA-2-dependent manner. These data suggest that FRA-2 is involved in the transcriptional control of neuroprotective genes and in the switch from aerobic to anaerobic metabolism.
Butler
Tanya L
TL
Department of Pharmacology and Therapeutics, University of South Florida, Tampa, FL 33612, USA.
Pennypacker
Keith R
KR
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051921:Fos-Related Antigen-2; D020869:Gene Expression Profiling; D005799:Genes, Dominant; D020932:Nerve Growth Factor / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D016716:PC12 Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
D015415:Biomarkers; D004268:DNA-Binding Proteins; D051921:Fos-Related Antigen-2; C497874:Fosl2 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; D020932:Nerve Growth Factor