title: Phenotypic and genotypic characterization of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Denmark.
abstract: In the study presented here 26 recent Danish clinical isolates of prolyliminopeptidase (PIP)-negative Neisseria gonorrhoeae were phenotypically and genotypically characterized to investigate whether one or more PIP-negative strains are circulating in the Danish community. The profiles of these isolates were compared with those of three isolates from a recent outbreak of PIP-negative N. gonorrhoeae infection in the UK. Twenty-five of the Danish isolates and all three UK isolates had similar antibiograms and were designated serovar IB-4. Genotypic characterization by pulsed-field gel electrophoresis, porB1b gene sequencing, and opa-typing revealed that these isolates were indistinguishable or closely related. The results indicate that at least one PIP-negative N. gonorrhoeae strain is currently circulating in the Danish community, and this strain is indistinguishable from the one that caused an outbreak in the UK.
authors.affiliation: Department of Clinical Microbiology, H:S Hvidovre Hospital, Kettegaard allé 30, 2650 Hvidovre, Denmark,
authors.forename: H
authors.initials: H
authors.lastname: Fjeldsøe-Nielsen
authors.forename: M
authors.initials: M
authors.lastname: Unemo
authors.forename: H
authors.initials: H
authors.lastname: Fredlund
authors.forename: S V
authors.initials: SV
authors.lastname: Hjorth
authors.forename: L M
authors.initials: LM
authors.lastname: Berthelsen
authors.forename: H M
authors.initials: HM
authors.lastname: Palmer
authors.forename: A
authors.initials: A
authors.lastname: Friis-Møller
mesh_terms: D000328:Adult; D000626:Aminopeptidases; D003718:Denmark / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006069:Gonorrhea / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D018272:Porins / Q000235:genetics
chemical_list: D018272:Porins; C057699:porin protein, Neisseria; D000626:Aminopeptidases; C022450:prolyl aminopeptidase