Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry.
In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isobaric with ions commonly used for the detection of cyclosporin A and cyclosporin D, respectively. In this article it is demonstrated that limited chromatography with co-elution of such metabolites together with cyclosporin A and cyclosporin D can lead to incorrect results.
Vogeser
Michael
M
Institute of Clinical Chemistry, Hospital of the University of Munich, Germany. michael.vogeser@med.uni-muenchen.de
Spöhrer
Ute
U
D016477:Artifacts; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D016572:Cyclosporine / Q000097:blood* / Q000592:standards; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D016572:Cyclosporine