Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells.
Radial glia function during CNS development both as neural progenitors and as a scaffolding supporting neuronal migration. To elucidate pathways involved in these functions, we mapped in vivo the promoter for Blbp, a radial glial gene. We show here that a binding site for the Notch effector CBF1 is essential for all Blbp transcription in radial glia, and that BLBP expression is significantly reduced in the forebrains of mice lacking the Notch1 and Notch3 receptors. These results identify Blbp as the first predominantly CNS-specific Notch target gene and suggest that it mediates some aspects of Notch signaling in radial glia.
Anthony
Todd E
TE
Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.
Mason
Heather A
HA
Gridley
Thomas
T
Fishell
Gord
G
Heintz
Nathaniel
N
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005455:Fluorescent Antibody Technique; D005697:Galactosides; D018507:Gene Expression Regulation, Developmental; D006651:Histocytochemistry; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D007211:Indoles; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D051880:Receptors, Notch; D015398:Signal Transduction / Q000502:physiology*
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005697:Galactosides; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D007211:Indoles; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C497571:Rbpj protein, mouse; D051880:Receptors, Notch; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside