title: [Evaluation of measurement uncertainty for the determination of ginsenosides in Radix ginseng by HPLC].
abstract: OBJECTIVE
  To set out the procedure for estimation of measurement uncertainty for the determination of ginsenosides R(g1), Re and R(b1) in Radix ginseng by HPLC.
  
  
  METHODS
  To facilitate the identification and analysis of the uncertainty sources arising from the procedure of analysis, a cause and effect diagram was constructed and simplified. Each uncertainty component whether associated with individual sources or with the combined effects of several sources, was evaluated with respect to the significance of its contribution to the overall measurement uncertainty and was expressed as standard uncertainty. All the standard uncertainties were then combined according to the appropriate rules to give a combined standard uncertainty and an expanded standard uncertainty. Results The expanded standard uncertainties for the HPLC determination of ginsenoside R(g1), Re, and R(b1), are 0.12c, 0.14c and 0.13c, respectively.
  
  
  CONCLUSIONS
  Measurement uncertainty is applicable to set the limit of the ginsenosides in Radix ginseng. The establishment of the methodology for the evaluation of measurement uncertainty is important to the studies of Chinese materia medica standards.
authors.affiliation: School of Chemistry and Pharmaceutics, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Guo-an
authors.initials: GA
authors.lastname: Luo
authors.forename: Zhong-zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Kelvin K C
authors.initials: KK
authors.lastname: Chan
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1