title: Microbial structural diversity estimated by dilution-extinction of phenotypic traits and T-RFLP analysis along a land-use intensification gradient.
abstract: The present work tested whether the relationship between functional traits and inoculum density reflected structural diversity in bacterial communities from a land-use intensification gradient applying a mathematical model. Terminal restriction fragment length polymorphism (T-RFLP) analysis was also performed to provide an independent assessment of species richness. Successive 10-fold dilutions of a soil suspension were inoculated onto Biolog GN(R) microplates. Soil bacterial density was determined by total cell and plate counts. The relationship between phenotypic traits and inoculum density fit the model, allowing the estimation of maximal phenotypic potential (Rmax) and inoculum density (KI) at which Rmax will be half-reduced. Though Rmax decreased with time elapsed since clearing of native vegetation, KI remained high in two of the disturbed sites. The genetic pool of bacterial community did not experience a significant reduction, but the active fraction responding in the Biolog assay was adversely affected, suggesting a reduction in the functional potential.
authors.affiliation: Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina. egomez@fcagr.unr.edu.ar
authors.forename: Elena
authors.initials: E
authors.lastname: Gomez
authors.forename: Jay L
authors.initials: JL
authors.lastname: Garland
authors.forename: Michael S
authors.initials: MS
authors.lastname: Roberts
mesh_terms: D001419:Bacteria / Q000235:genetics*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis*; D017753:Ecosystem; D014644:Genetic Variation; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S