Immobilization of glucose oxidase onto gelatin for biosensor construction.
The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized using chemical and electrochemical methods. Temperature, pH, cross-linking agent concentration, enzyme concentration, kinetic properties, reusability and the effect of electro-active compounds were among the parameters studied. The response time of the glucose oxidase biosensor is 90 s, the detection limit is below 1 mmol/dm3 and the sensor can be used 20 times within a 2-month period without losing its stability.
Emregul
Emel
E
Department of Chemistry, Faculty of Science, Ankara University, 06100 Ankara, Turkey. eemregul@yahoo.com
Sungur
Sibel
S
Akbulut
Ural
U
D000085:Acetates / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D002857:Chromium / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D004563:Electrochemistry; D004566:Electrodes; D004572:Electrolysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000085:Acetates; D000814:Aniline Compounds; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; C416807:polyaniline; D002857:Chromium; D005780:Gelatin; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen