title: DNA damage in leukocytes of workers occupationally exposed to arsenic in copper smelters.
abstract: Inorganic arsenic (i-As) is a known human carcinogen; however, humans continue to be exposed to i-As in drinking water and in certain occupational settings. In this study, we used the Comet assay to evaluate DNA damage in the somatic cells of workers from three Polish copper smelters who were occupationally exposed to i-As. Blood samples were collected from 72 male workers and 83 unexposed male controls and used for the detection of DNA damage, oxidative DNA damage, and DNA damage after a 3-hr incubation in culture. Urine samples were collected to assess the level of exposure. The mean concentration of arsenic metabolites in urine [the sum of arsenite (AsIII), arsenate (AsV), monomethylarsenate (MMA) and dimethylarsenate (DMA)] and the concentrations of DMA (the main metabolite in urine) were higher in workers than in controls, but the differences were not statistically significant. By contrast, the level of DNA damage, expressed as the median tail moment, was significantly higher in the leukocytes of workers than in the controls. Comet assays conducted with formamidopyrimidine glycosylase (FPG) digestion to detect oxidative DNA damage indicated that oxidative lesions were present in leukocytes from both the exposed and control groups, but the levels of damage were significantly higher among the workers. Incubation of the cells in culture resulted in a significant reduction in the levels of DNA damage, especially among leukocytes from the workers, suggesting that the DNA damage was subject to repair. Our findings indicate that copper smelter workers have increased levels of DNA damage in somatic cells, suggesting a potential health risk for the workers. Although i-As was present in air samples from the smelters and in urine samples from workers, no clear association could be made between i-As exposure and the DNA damage.
authors.affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland. jadwigap@imp.lodz.pl
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Palus
authors.forename: Dobroslawa
authors.initials: D
authors.lastname: Lewinska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Dziubaltowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Stepnik
authors.forename: Jens
authors.initials: J
authors.lastname: Beck
authors.forename: Konrad
authors.initials: K
authors.lastname: Rydzynski
authors.forename: Robert
authors.initials: R
authors.lastname: Nilsson
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D001151:Arsenic / Q000652:urine*; D002101:Cacodylic Acid / Q000652:urine; D020552:Comet Assay; D003300:Copper / Q000737:chemistry*; D004249:DNA Damage; D003627:Data Interpretation, Statistical; D004784:Environmental Monitoring / Q000706:statistics & numerical data; D006801:Humans; D007962:Leukocytes / Q000737:chemistry* / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D008906:Mining; D016273:Occupational Exposure / Q000706:statistics & numerical data; D011044:Poland
chemical_list: D000395:Air Pollutants, Occupational; D003300:Copper; D002101:Cacodylic Acid; D001151:Arsenic