Chemopreventive effects of Paullinia cupana Mart var. sorbilis, the guaraná, on mouse hepatocarcinogenesis.
Guaraná (Paullinia cupana) is originally from Amazon, Brazil. Its effects on mouse hepatocarcinogenesis have been investigated in this study. Mice were treated with N-nitrosodiethylamine (DEN), received three different doses of P. cupana added to commercial food, and euthanized after 25 weeks. Gross lesions were quantified, and preneoplastic lesions (PNL) were histologically measured. Cellular proliferation was evaluated by immunobloting for the proliferating cell nuclear antigen (PCNA). The incidence and multiplicity of macroscopic lesions were reduced. The PNL number and PCNA expression were reduced in the highest P. cupana dose. According to these results, guaraná presented inhibitory effects on DEN hepatocarcinogenesis in mice.
Fukumasu
Heidge
H
Laboratory of Experimental Oncology, Department of Pathology, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr Orlando Marques de Paiva, 87. CEP 05508-900 São Paulo, SP, Brazil.
da Silva
Tereza Cristina
TC
Avanzo
José Luis
JL
de Lima
Cyntia Esteves
CE
Mackowiak
Ivone Isabel
II
Atroch
André
A
de Souza Spinosa
Helenice
H
Moreno
Fernando Salvador
FS
Dagli
Maria Lucia Zaidan
ML
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D032126:Paullinia; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D011230:Precancerous Conditions / Q000517:prevention & control; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D002110:Caffeine