[Apoptosis of phagocytes as one of the probable mechanisms of the pathogenetic action of Yersinia pestis "mouse" toxin].
Apoptosis was evaluated by characteristic morphological changes of cells in preparations stained with histological dyes and in live preparations, as well as by DNA degradation, colorimetrically detected with the use of the diphenylamine reagent. "Mouse toxin" (MT) was found to have a pronounced apoptogenic action with respect to the phagocytic cells of mice, but not guinea pigs. Macrophages were affected by this action stronger than neutrophils, and in both cases this effect was dose dependent. As the dose of MT decreased to 0.01 microg/ml, the proportion of cells dying as the result of apoptosis increased, the necrotic type of damage was almost absent. On the contrary, as MT concentration rose to 1.0 microg/ml and over, the proportion of phagocytes dying due to necrosis increased with a decrease in the number of cells in which the process of apoptosis started. The results of the study are indicative of the fact that the mechanisms programming the death of cells under the action of MT on macrophages and neutrophils took part in the process, which, in its turn, determined their role in the pathogenesis of plague.
Vasil'eva
G I
GI
Kiseleva
A K
AK
Mishan'kin
M B
MB
Kozlovskiĭ
V N
VN
Mishan'kin
B N
BN
D000818:Animals; D000942:Antigens, Bacterial; D017209:Apoptosis / Q000276:immunology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000276:immunology; D010586:Phagocytes / Q000276:immunology* / Q000473:pathology; D010930:Plague / Q000209:etiology; D018089:Superantigens / Q000494:pharmacology*; D015010:Yersinia pestis / Q000276:immunology* / Q000472:pathogenicity
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D018089:Superantigens