Bacterial colonization on different suture materials--a potential risk for intraoral dentoalveolar surgery.
In this in vivo and in vitro study on resorbable (Monocryl and nonresorbable (Deknalon) monofilament sutures used in intraoral dentoalveolar surgery the bacterial colonization was compared. For the in vivo study the sutures were applied in 11 patients during dental surgery. Eight days postoperative the sutures were removed and the adhered bacteria were isolated and identified by biochemistry, morphology, antibiotic susceptibility, and gas chromatography. The colonization was studied by scanning electron microscopy. Aerobic and anaerobic bacteria were isolated in nearly equal colony-forming units (cfu) on each suture. In comparison with Monocryl about 15% more aerobic and anaerobic strains were isolated on Deknalon. Regarding the pathogens only, about three times more anaerobic strains were isolated on both sutures in total. Additionally, more pathogens were found on Deknalon than on Monocryl (aerobic >40%, anaerobic >25%). The variety of bacteria correspond with purulent infections, not with normal oral flora. Intraindividual comparisons of cfu showed differences in dependence of the patient as described for subgingivale plaques. For the in vitro study the sutures were incubated with Streptococcus intermedius and Prevotella intermedia for 0.5 h. Scanning electron microscopy was performed to examine qualitatively the level of bacterial adherence. After 0.5 h the bacteria adhered very well. The colonization rate of Streptococcus intermedius on both sutures was similar. Coccoid bacteria within biofilms were seen. The growth of Prevotella intermedia was much better on Deknalon than on Monocryl. The risk of bacteremia at the time of suture removal is discussed.
Otten
J-E
JE
Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Albert-Ludwigs-Universität Freiburg, Germany. otten@zmk2.uk1.uni-freiburg.de
Wiedmann-Al-Ahmad
M
M
Jahnke
H
H
Pelz
K
K
D001419:Bacteria / Q000648:ultrastructure; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002849:Chromatography, Gas; D015921:Dental Implants / Q000382:microbiology*; D004146:Dioxanes / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009757:Nylons; D011091:Polyesters / Q000737:chemistry; D011183:Postoperative Complications; D018713:Prevotella intermedia / Q000378:metabolism; D012306:Risk; D013234:Stem Cells; D034367:Streptococcus intermedius / Q000378:metabolism; D013529:Surgical Wound Dehiscence; D013530:Surgical Wound Infection; D013536:Suture Techniques; D013537:Sutures; D013997:Time Factors
D001672:Biocompatible Materials; D015921:Dental Implants; D004146:Dioxanes; D009757:Nylons; D011091:Polyesters; C095495:glycolide E-caprolactone copolymer