Study of tumor cell invasion by Fourier transform infrared microspectroscopy.
Lung cancer is usually fatal once it becomes metastatic. However, in order to develop metastases, a tumor usually invades the basal membrane and enters the vascular or lymphatic system. In this study, a three-dimensional artificial membrane using collagen type I, one of the main components of basal membranes, was established in order to investigate tumor cell invasion. Lung cancer cell line CALU-1 was seeded on this artificial membrane and cell invasion was studied using the Fourier transform infrared (FTIR) imaging technique. This approach allowed identification of tumor cells invading the collagen type I membrane by means of their infrared spectra and images. The mapping images obtained with FTIR microspectroscopy were validated with standard histological section analysis. The FTIR image produced using a single wavenumber at 1080 cm(-1), corresponding to PO2- groups in DNA from cells, correlated well with the histological section, which clearly revealed a cell layer and invading cells within the membrane. Furthermore, the peaks corresponding to amide A, I, and II in the spectra of the invading cells shifted compared to the noninvading cells, which may relate to the changes in conformation and/or heterogeneity in the phenotype of the cells. The data presented in this study demonstrate that FTIR microspectroscopy can be a fast and reliable technique to assess tumor invasion in vitro.
Yang
Ying
Y
Institute for Science and Technology in Medicine, Keele University, Stoke-on-Trent ST4 7QB, UK. bea00@keele.ac.uk
Sulé-Suso
Josep
J
Sockalingum
Ganesh D
GD
Kegelaer
Gregory
G
Manfait
Michel
M
El Haj
Alicia J
AJ
D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D045744:Cell Line, Tumor; D024042:Collagen Type I; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D008567:Membranes, Artificial; D008853:Microscopy / Q000379:methods; D009361:Neoplasm Invasiveness; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D024042:Collagen Type I; D008567:Membranes, Artificial