Inhibition of porcine small intestinal sucrase by valienamine.
Valienamine, an aminocyclitol, has been isolated from the enzymolysis broth of validamycins. The absolute configuration of valienamine is similar to that of alpha-D-glucose. The inhibitory effect of this amino-sugar analog of alpha-D-glucose, valienamine, on porcine small intestinal sucrase was examined. Valienamine was found to be potent, competitive reversible inhibitor of porcine small intestinal sucrase in vitro with an IC50 value of 1.17 x 10(-3)M. Valienamine also exhibited dose-dependent, instantaneous inhibition of porcine small intestinal sucrase. The inhibition of porcine small intestinal sucrase by valienamine was pH-independent.
Zheng
Yu-Guo
YG
Institute of Bioengineering, State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China. zhengyg@zjut.edu.cn
Shentu
Xu-Ping
XP
Shen
Yin-Chu
YC
D000818:Animals; D053138:Cyclohexenes; D006595:Hexosamines / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology*; D013393:Sucrase / Q000037:antagonists & inhibitors*; D013552:Swine
D053138:Cyclohexenes; D006595:Hexosamines; C030791:valienamine; D007294:Inositol; C003749:validamycins; D013393:Sucrase