[Isolation and preparation of sesquiterpenols from the Japanese cedar, Cryptomeria japonica D. Don, by preparative reversed-phase high performance liquid chromatography and its characterization].
A method for the isolation of sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by preparative reversed-phase high performance liquid chromatography (RP-HPLC) was established. The preparation of the sesquiterpenols was carried out on a preparative liquid chromatograph with a YWC-Pack C18 column, and the mobile phase was isopropanol-methanol-hexane-water (50:35:10:5, v/v) at a flow rate of 5.0 mL/min. The two sesquiterpenols, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one, were separated by liquid chromatography and identified by spectroscopic analyses (ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS)). The purities of the two sesquiterpenols were 98.7% and 99.1%, respectively.
Chen
Xiaohui
X
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. cxh88820022002@yahoo.com.cn
Zhang
Huifen
H
Bi
Kaishun
K
Kim
Chul-Sa
CS
Horiike
Michio
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase; D032485:Cryptomeria / Q000737:chemistry*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D018514:Plant Structures / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis* / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
C442121:2,7(14),10-bisabolatrien-1-ol-4-one; D007202:Indicators and Reagents; D012717:Sesquiterpenes; C442120:cubebol