title: Counting absolute number of lymphocytes in quail whole blood by flow cytometry.
abstract: In a previous study, we reported a new method for counting quail blood cells. After quail blood cells were stained with fluorescent lipophilic dye (DiOC6(3)), absolute counts of erythrocytes, granulocytes, and monocytes were obtained by means of flow cytometry (FC). The FC method has the potential for application to avian blood cells count; however, the method was unable to distinguish between lymphocytes and thrombocytes. In the present study, we improved the FC method to obtain separate counts of lymphocytes using DiOC5(3). After quail blood cells were stained with DiOC5(3), the cells were measured with FC. Each blood cell type was distinguished by means of their typical FL-1 (green fluorescence) and SSC (side scatter). Absolute numbers of erythrocytes, granulocytes, monocytes and lymphocytes in whole blood were obtained. The improved FC analysis worked equally well with chicken (Gallus gallus) and goose (Anser cygnoides) blood.
authors.affiliation: Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fugisawa, Kanagawa, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Uchiyama
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
authors.forename: Osamu
authors.initials: O
authors.lastname: Kai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Uwatoko
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D002232:Carbocyanines; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D018655:Lymphocyte Count / Q000379:methods; D008214:Lymphocytes; D011784:Quail / Q000097:blood*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C018528:3,3'-dipentyl-2,2'-oxacarbocyanine