Single cell electrophoresis in determining cell death: potential for use in organ transplant research.
Viability of donor tissues is essential for the success of organ transplantation. Although much work has been done in the field of organ preservation, currently there are few objective methods for evaluating transplant organ viability, and thus preservation efficiency. In the field of cancer biology, single-cell gel electrophoresis (SCGE) is a technique commonly used to measure the efficacy of anti-tumor treatments by measuring the breakdown of tumor cell deoxyribonucleic acid (DNA). This assay has recently been applied to various organs from a postmortem porcine animal model, and cells were found to undergo postmortem breakdown in a way similar to apoptosis-induced DNA fragmentation. Collections of cells from each organ reached levels indicative of non-viability as the postmortem interval (PMI) progressed. The rates of cellular DNA degradation were found to be specific to each organ type at a given ambient temperature. We believe that following the application of various preservation techniques, SCGE assay has the potential to provide a clear indication of cell viability in an organ destined for transplant. As a readily available viability assay, this technique could provide transplant researchers with a useful tool to quantify the efficacy of their experimental organ preservation techniques.
Johnson
Laura A
LA
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada. laura@brc.ubc.ca
Ferris
James A J
JA
D000818:Animals; D016923:Cell Death; D020552:Comet Assay / Q000379:methods*; D053938:DNA Fragmentation; D006801:Humans; D007668:Kidney / Q000166:cytology; D007962:Leukocytes / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology; D009926:Organ Preservation; D011180:Postmortem Changes; D013552:Swine; D009927:Tissue and Organ Procurement