Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in North Argentina.
Sixteen Leishmania stocks, 15 isolated from patients with cutaneous (CL), mucocutaneous (MCL), or recurrent cutaneous leishmaniasis, plus one from a dog with CL in Salta and Corrientes Provinces, Argentina, were studied by multilocus enzyme electrophoresis. Thirteen of the stocks from humans were grouped in two zymodemes; nine termed as KMS 1, four as KMS 2, and assigned to Leishmania (Viannia) braziliensis. Two additional stocks from CL cases expressed a KMS 4 enzyme profile, corresponding to L. (V.) guyanensis. Although the parasites from the dog were also assigned to L. (V.) braziliensis, its zymodeme, KMS 3, was not expressed in any of the current human isolates. The characterization of Leishmania from a dog was done for the first time in Argentina. The importance of the intraspecific polymorphism in the induction of clinical forms and in the host-reservoir concept is briefly discussed, based on the zymodeme data of isolates from humans and dogs. The presence of L. (V.) guyanensis was confirmed in the country.
Marco
Jorge D
JD
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan. marcojd@med.kochi-ms.ac.jp
Barroso
Paola A
PA
Calvopiña
Manuel
M
Kumazawa
Hideo
H
Furuya
Masato
M
Korenaga
Masataka
M
Cajal
Silvana P
SP
Mora
María C
MC
Rea
María M J
MM
Borda
C Edgardo
CE
Basombrío
Miguel A
MA
Taranto
Néstor J
NJ
Hashiguchi
Yoshihisa
Y
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary; D010802:Phylogeny; D013045:Species Specificity