title: Genome replication and progeny virion production of herpes simplex virus type 1 mutants with temperature-sensitive lesions in the origin-binding protein.
abstract: Genome replication of herpes simplex viruses (HSV) in cultured cells is thought to be started by the action of the virus-encoded origin-binding protein (OBP). In experiments using two HSV-1 mutants with temperature-sensitive lesions in the helicase domain of OBP, we demonstrated that this function is essential during the first 6 hours of the lytic cycle. Once DNA synthesis has started, this function is no longer required, suggesting that origin-driven initiation of viral DNA replication is a single event rather than a continuous process.
authors.affiliation: Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schildgen
authors.forename: Sascha
authors.initials: S
authors.lastname: Gräper
authors.forename: Johannes
authors.initials: J
authors.lastname: Blümel
authors.forename: Bertfried
authors.initials: B
authors.lastname: Matz
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D004279:DNA, Viral / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology*; D007700:Kinetics; D009154:Mutation; D013696:Temperature; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C055789:origin-binding proteins, viral; D004265:DNA Helicases