title: [Experimental investigation of postoperative use of medication in refractive surgery].
abstract: OBJECTIVE
  To harvest microorganisms from the microkeratome after LASIK. To induce experimental infectious keratitis and investigate the use of routine postoperative medication.
  
  
  METHODS
  During eight consecutive days, we cultured the plate of the microkeratome after routine LASIK. Dilutions of the most frequent microorganism was prepared according to the MacFarland scale. Ten microliters were injected into both corneas of eight guinea pigs. The right cornea was used as control and the left cornea was treated with 0.3% ciprofloxacin associated with dexamethasone and ketorolac tromethamine every 4 hours for 24 hours (group 2). The animals were sacrificed and the corneas were divided into two halves, which were sent to analyzed by the microbiology and histopathology laboratory.
  
  
  RESULTS
  In the control group, Staphylococcus aureus could be retrieved from all corneas, while in group 2, only 50% showed positive cultures (p=0.0128). Histopathology concerning polymorphonuclear cells showed an intense degree of inflammation in group 1 (p=0.0203) and regarding monomorphonuclear cells, it was also more intense in group 1 (p=0.0051).
  
  
  CONCLUSIONS
  This study suggests that the use of wide-spectrum antibiotic associated with anti-inflammatory eye drops has a significant effect on the reduction of a potential infectious and inflammatory process after refractive surgery.
authors.affiliation: Hospital Universitário Evangélico de Curitiba. saliw@terra.com.br
authors.forename: Sâmia Ali
authors.initials: SA
authors.lastname: Wahab
authors.forename: Hamilton
authors.initials: H
authors.lastname: Moreira
authors.forename: Michele
authors.initials: M
authors.lastname: Buquera
authors.forename: Luciane
authors.initials: L
authors.lastname: Moreira
authors.forename: Alessandra Castelano
authors.initials: AC
authors.lastname: Daros
authors.forename: Cínara Sakuma de
authors.initials: CS
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002939:Ciprofloxacin / Q000627:therapeutic use; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D006168:Guinea Pigs; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology; D020731:Keratomileusis, Laser In Situ; D020911:Ketorolac Tromethamine / Q000627:therapeutic use; D011183:Postoperative Complications / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000150:complications / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D020911:Ketorolac Tromethamine; D002939:Ciprofloxacin; D003907:Dexamethasone