Mechanism and applications of phosphite dehydrogenase.
Phosphite dehydrogenase catalyzes the NAD+-dependent oxidation of hydrogen phosphonate (common name phosphite) to phosphate in what amounts to a formal phosphoryl transfer reaction from hydride to hydroxide. This review places the enzyme in the context of phosphorus redox metabolism in nature and discusses the results of mechanistic investigations into its reaction mechanism. The potential of the enzyme as a NAD(P)H cofactor regeneration system is discussed as well as efforts to engineer the cofactor specificity of the protein.
Relyea
Heather A
HA
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Matthews Ave., Urbana, IL 61801, USA.
van der Donk
Wilfred A
WA
D000595:Amino Acid Sequence; D002384:Catalysis; D008969:Molecular Sequence Data; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010710:Phosphates / Q000138:chemical synthesis / Q000378:metabolism; D017905:Phosphites / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D010710:Phosphates; D017905:Phosphites; D009243:NAD; D009247:NADH, NADPH Oxidoreductases; C429539:NAD phosphite oxidoreductase