Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity.
A method of assessing chemosensitivity of tissue utilizing tissue fluorescence and image analysis was implemented to provide a rapid and quantitative means of assessing the effect of drugs on tissue metabolic activity and proliferative capacity. The fluorescent microscopic image captured by a silicon-intensified target (low-light-detecting) camera and linked to an image- processing unit was measured for fluorescent brightness and tumor image area. An established rodent model served to characterize the system's ability to measure serially the tumor's metabolic activity and growth. Further studies on fresh human tumors were conducted with a novel topoisomerase II inhibitor, NC-190. Tumor image area and fluorescent brightness were measured 24 h pretreatment, 48 h posttreatment, and 48 h post-drug removal. Fifty-five percent (28/51) of fresh human tumors showed sensitivity to 48-h exposure to 10, 30, or 100 microM NC-190. The potential benefit of this technique is the ability to predict the response of tumors to chemotherapeutic agents as a laboratory tool for preclinical drug evaluation and clinically prior to the commencement of therapy.
Gibbs
John F
JF
Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA.
Rustum
Youcef M
YM
Slocum
Harry K
HK
D019813:1,2-Dimethylhydrazine; D000230:Adenocarcinoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001706:Biopsy; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007580:Jejunal Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D010619:Phenazines / Q000494:pharmacology; D051381:Rats; D059005:Topoisomerase II Inhibitors
D000970:Antineoplastic Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010619:Phenazines; D059005:Topoisomerase II Inhibitors; C059048:NC 190; D019813:1,2-Dimethylhydrazine; C018506:diacetylfluorescein