DNA damage of germ cell of rat induced by nitrotoluene chemicals.
2,4-dinitrotoluene(2,4-DNT), 2,6-dinitrotoluene(2,6-DNT) and 4-nitrotoluene(4-NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single-strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4-DNT, 2,6-DNT and 4-NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.
Yang
Li
L
Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China. yangl@igsnrr.ac.cn
Xu
Jing-bo
JB
Zhen
Lin
L
Sun
Zhi-wei
ZW
Shi
Long
L
Jin
Ming-hua
MH
Liu
Xiao-mei
XM
D000818:Animals; D004249:DNA Damage; D004136:Dinitrobenzenes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D005854:Germ Cells / Q000187:drug effects*; D016014:Linear Models; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014050:Toluene / Q000031:analogs & derivatives* / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D004136:Dinitrobenzenes; D014874:Water Pollutants, Chemical; D014050:Toluene; C016403:2,4-dinitrotoluene; C017572:4-nitrotoluene; C023514:2,6-dinitrotoluene