Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells.
Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5'-flanking region of human MEF gene and identified a TATA-less promoter region at -204/-54 which contains 4 putative binding sites for Sp1, two of which are essential in up-regulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene.
Koga
Tomoaki
T
Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan.
Suico
Mary Ann
MA
Nakamura
Hideaki
H
Taura
Manabu
M
Lu
Zhuo
Z
Shuto
Tsuyoshi
T
Okiyoneda
Tsukasa
T
Kai
Hirofumi
H
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D015536:Down-Regulation; D036383:Ephrin-A2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors / Q000502:physiology*
D036383:Ephrin-A2; D016329:Sp1 Transcription Factor; D004247:DNA