title: [Optimization of transfection properties of DNA-lysine dendrimer complexes].
abstract: We studied the possibility of optimizing the DNA transfection properties of carriers based on lysine dendrimers of the third and the fifth generation, including those containing a chloroacetyl or a lipophilic palmitoyl moiety at C-end. The use of lysosome-destroying antibiotic chloroquine and an amphipathic polycationic nonadecapeptide JTS-1 was found to enhance the DNA transfecting properties of the lysine dendrimers. The triple complex including DNA, a lysine dendrimer of the third generation modified with lipophylic moieties of palmitic acid at its C-end, and JTS-1 was shown to be comparable in its transfecting activity to a complex containing Escort, a commercial cationic liposome carrier.
authors.forename: G P
authors.initials: GP
authors.lastname: Vlasov
authors.forename: E A
authors.initials: EA
authors.lastname: Lesina
authors.forename: V I
authors.initials: VI
authors.lastname: Korol'kov
authors.forename: I A
authors.initials: IA
authors.lastname: Gur'ianov
authors.forename: N V
authors.initials: NV
authors.lastname: Baianova
authors.forename: A N
authors.initials: AN
authors.lastname: Baranov
authors.forename: A V
authors.initials: AV
authors.lastname: Kisilev
authors.forename: V S
authors.initials: VS
authors.lastname: Baranov
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D045744:Cell Line, Tumor; D002738:Chloroquine / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D010455:Peptides; D002738:Chloroquine; D004247:DNA; D001616:beta-Galactosidase; D008239:Lysine