title: Detection of JC virus DNA sequences in brain tumors in pediatric patients.
abstract: OBJECTIVE
  The JC virus is a human neurotropic polyomavirus that causes progressive multifocal leukoencephalopathy and is closely related to simian virus 40. Several recent reports have indicated a possible association between the JC virus and the development of various human brain tumors. The authors examined the presence of JC virus DNA sequences in primary brain tumors in pediatric patients to evaluate the hypothesis that particular brain tumors can arise in the pediatric population as a consequence of infection with the JC virus.
  
  
  METHODS
  Genomic DNA sequences were isolated from 62 brain tumors (32 medulloblastomas, 18 ependymomas, five choroid plexus papillomas, and seven pilocytic astrocytomas) and analyzed for the presence of JC virus DNA by Southern blot hybridization and direct sequencing. The JC virus DNA sequence was detected in five ependymomas and one choroid plexus papilloma. Immunohistochemical studies revealed nuclear expression of the large T-antigen in a choroid plexus papilloma. None of the medulloblastomas or pilocytic astrocytomas contained JC virus DNA.
  
  
  CONCLUSIONS
  The results of this study provide molecular evidence of the association between JC virus and the development of certain ependymomas and choroid plexus papillomas.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Saga University, Saga, Japan. okamotoh@post.saga-med.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mineta
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ueda
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakahara
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shiraishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Tamiya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tabuchi
mesh_terms: D000957:Antigens, Viral, Tumor / Q000235:genetics; D001254:Astrocytoma / Q000473:pathology / Q000821:virology; D001483:Base Sequence; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000473:pathology / Q000821:virology*; D002528:Cerebellar Neoplasms / Q000473:pathology / Q000821:virology; D002551:Cerebral Ventricle Neoplasms / Q000473:pathology / Q000821:virology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004806:Ependymoma / Q000473:pathology / Q000821:virology; D005260:Female; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007577:JC Virus / Q000235:genetics*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000473:pathology / Q000821:virology*; D008297:Male; D008527:Medulloblastoma / Q000473:pathology / Q000821:virology; D020288:Papilloma, Choroid Plexus / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D015173:Supratentorial Neoplasms / Q000473:pathology / Q000821:virology
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral