[Determination of angiotensin converting enzyme inhibitor activity by high performance liquid chromatography].
A high performance liquid chromatographic method to determine angiotensin-converting enzyme inhibitor activity in vitro was established by using N-hippuryl-His-Leu tetrahydrate as the reaction substrate and hippuric acid as the reaction product. The chromatographic conditions were as follows: column, ZORBAX SB-C18 (4.6 mm i. d. x 150 mm, 5 microm); column temperature, 25 degrees C; mobile phase, acetonitrile-distilled water (25:75, v/v, both containing 0.05% (v/v) trifluoroacetic acid and 0.1% (v/v) triethylamine); flow rate, 0.5 mL/min; detection wavelength, 228 nm. An excellent linearity over the range of 0.005-1.000 mmol/L (r = 0.9999) was observed. The detection limit was 0.50 micromol/L. The recoveries of hippuric acid ware 99.48%-105.64%, with a relative standard deviation (RSD) of 2.20% (n = 6). It is a simple, precise and reliable assay method for developing antihypertension drugs.
Wu
Qiongying
Q
College of Biology and Environment Engineering, Jiangsu University, Zhenjiang 212013, China.
Ma
Haile
H
Luo
Lin
L
Wu
Shouyi
S
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006626:Hippurates / Q000737:chemistry; D007202:Indicators and Reagents
D000806:Angiotensin-Converting Enzyme Inhibitors; D006626:Hippurates; D007202:Indicators and Reagents; C030514:hippuric acid