NF-kappaB activation in Kupffer cells after partial hepatectomy.
The transcription factor nuclear factor-kappaB (NF-kappaB) is activated during liver regeneration after partial hepatectomy. However, the physiological role and cellular localization of NF-kappaB activation are unresolved. In this study, we used an adenoviral vector expressing a mutated form of IkappaBalpha to inhibit NF-kappaB activity during liver regeneration. After partial hepatectomy in mice, introduction of Ad5IkappaB, but not a control virus (Ad5GFP), resulted in increased liver injury and decreased hepatocyte proliferation. Hepatocyte apoptosis was not observed. To investigate the kinetics and cellular localization of NF-kappaB-induced transcription during liver regeneration, we generated a transgenic mouse expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-kappaB cis elements (cis-NF-kappaB-EGFP). During liver regeneration, EGFP expression was detected within 12 h and was primarily located in Kupffer cells. Our data demonstrate that activation of NF-kappaB initially occurs in Kupffer cells after partial hepatectomy in mice.
Yang
Liu
L
Dept. of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA.
Magness
Scott T
ST
Bataller
Ramon
R
Rippe
Richard A
RA
Brenner
David A
DA
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006498:Hepatectomy / Q000662:veterinary*; D007700:Kinetics; D007728:Kupffer Cells; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000096:biosynthesis* / Q000502:physiology*; D014158:Transcription, Genetic
D016328:NF-kappa B; D049452:Green Fluorescent Proteins