title: Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis.
abstract: A cDNA encoding for a predicted small heat shock protein (sHSP), Tsol-sfISP35.6, has been isolated by antibody screening of a Taenia solium c-DNA library. The clone was a full-length sequence (1172 bp) with an open reading frame of 945 bp and encoded for a 314 amino acid protein with deduced molecular mass of 35.6 kDa, isoelectric point of 5.6 arid the characteristic HSP20/alpha-crystallin domain duplicated. It was highly conserved, with a high sequence similarity with other platyhelminth sHSPs. Western blot analysis, using serum from neurocysticercosis patients (NCC), indicated that the purified Tsol-sHSP35.6 expression product was immunogenic, while in indirect ELISA, using the purified Tsol-sHSP35.6 expression product as antigen and serum samples from pigs and humans, 80% of T. solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed.
authors.affiliation: Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ferrer
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: González
authors.forename: Mildred
authors.initials: M
authors.lastname: Foster-Cuevas
authors.forename: Maria Milagros
authors.initials: MM
authors.lastname: Cortéz
authors.forename: Iris
authors.initials: I
authors.lastname: Dávila
authors.forename: Mercedes
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.forename: Leslie J S
authors.initials: LJ
authors.lastname: Harrison
authors.forename: R Michael E
authors.initials: RM
authors.lastname: Parkhouse
authors.forename: Teresa
authors.initials: T
authors.lastname: Gárate
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000469:parasitology; D016366:Open Reading Frames / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D041201:Taenia solium / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D007106:Immune Sera; D011994:Recombinant Proteins