[Authentication of Zanthexylum bungeanum Maxin population and adulterants by analysis of rDNA ITS sequences].
OBJECTIVE
To study the difference of rDNA ITS sequences between Zanthexylum bungeanum populations and their adulterants in main habitants of China so as to provide molecular markers for identifying Zanthexylum bungeanum populations against adulterants.


METHODS
rDNA ITS regions (including ITS-1, 5.8S and ITS-2) of 7 populations of Zanthexylum bungeanum which are separate located in Gansu, Shanxi, Sichuan, Hebei provinces, and 3 adulterants were sequenced by PCR products sequencing method or clone sequencing method.


RESULTS
The sequences of rDNA ITS region of Zanthexylum bungeanum were reported for the first time, and the sequences of ITS region ranged from 619 to 620 bp, and the length difference amoung Zanthexylum bungeanum and their adulterants is 4 bp. There are 15 variable sites, 12 informative sites and 3 authenticable sites among Zanthexylum bungeanum populations. The difference of rDNA ITS regions amoung Zanthexylum bungeanum and their adulterants is obvious, the number of variable sites is 71.


CONCLUSIONS
The difference of rDNA ITS sequences can be used to authenticate accurately the populations of Zanthexylum bungeanum and their adulterants. These populations of Z. bungeanum which have close relationship always distribute in near geographic areas. The characteristics of rDNA ITS sequence can be used as good markers for authenticating Zanthexylum bungeanum populations form their adulterants.
Shen
Jie
J
I. Jiangsu Key Laboratory for Bioresource Technology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Ding
Xiao-yu
XY
Zhang
Wei-ming
WM
Bao
Shu-lin
SL
Chang
Jun
J
Tang
Feng
F
D001483:Base Sequence; D002681:China; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004340:Drug Contamination; D017753:Ecosystem; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D032106:Zanthoxylum / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer