title: Fluoride-releasing capacity and cariostatic effect provided by sealants.
abstract: This study evaluated the effect of sealants on enamel demineralization, focusing on physical protection of the sealed enamel and fluoride protection of the adjacent unsealed enamel. Occlusal fissures with areas measuring 12 mm2 were delimited in 48 extracted molars, randomly divided into 4 groups (n =12): 1) no sealing; 2) sealing with a resin-modified glass-ionomer (Vitremer, 3M ESPE); 3) sealing with a fluoride-releasing composite sealant (Clinpro Sealant, 3M ESPE); and 4) sealing with a non-fluoridated composite sealant (Concise, 3M ESPE). A 4-mm2 window was outlined on the buccal enamel for analysis of fluoride uptake. Following treatment, groups 2, 3 and 4 were subjected to 5-days of pH-cycling, while group 1 was kept in a moist environment at 37 degrees C. Fluoride uptake was assessed by dental biopsy, and the amount of fluoride released to the cycling solutions was determined by ion analysis. Enamel demineralization around the sealants was evaluated by cross-sectional micro-hardness analysis. Group 2 showed higher levels of fluoride release (P < 0.01) and uptake by enamel (P < 0.05), and lower levels of demineralization (P < 0.05) than groups 3 and 4. Group 3 exhibited reduced demineralization on unsealed enamel and provided fluoride uptake in a distant enamel area, while group 4 did not.
authors.affiliation: Cariology Area, Piracicaba School of Dentistry, University of Campinas, São Paulo, Brazil.
authors.forename: Maristela Maia
authors.initials: MM
authors.lastname: Lobo
authors.forename: Giovana Daniela
authors.initials: GD
authors.lastname: Pecharki
authors.forename: Cristiana
authors.initials: C
authors.lastname: Tengan
authors.forename: Débora Dias
authors.initials: DD
authors.lastname: da Silva
authors.forename: Elaine Pereira Silva
authors.initials: EP
authors.lastname: da Tagliaferro
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
mesh_terms: D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000493:pharmacokinetics*; D003188:Composite Resins / Q000737:chemistry / Q000627:therapeutic use; D003430:Cross-Sectional Studies; D003743:Dental Enamel / Q000378:metabolism*; D003799:Dental Stress Analysis; D005459:Fluorides / Q000032:analysis / Q000493:pharmacokinetics*; D005899:Glass Ionomer Cements / Q000627:therapeutic use; D006244:Hardness; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008963:Molar; D010895:Pit and Fissure Sealants / Q000737:chemistry* / Q000627:therapeutic use; D018709:Statistics, Nonparametric; D017001:Tooth Demineralization / Q000188:drug therapy*
chemical_list: D002327:Cariostatic Agents; C500418:Clinpro Sealant; D003188:Composite Resins; D005899:Glass Ionomer Cements; D010895:Pit and Fissure Sealants; C084550:Vitremer; D017438:Bisphenol A-Glycidyl Methacrylate; D005459:Fluorides