In vivo pretreatment with PGG-glucan fails to alter cytokine mRNA expression of equine peripheral blood mononuclear cells exposed to endotoxin ex vivo.
Twelve healthy horses were assigned to treatment or control groups. Treated horses received PGG-Glucan[ED-1] (1 mg/kg, IV) 24 hours prior to peripheral blood mononuclear cell (PBMC) isolation. PBMCs were isolated and incubated in the presence of lipopolysachharide (LPS). At 0, 6, 12, 24 and 48 hours messenger RNA (mRNA) was extracted. Reverse transcription polymerase chain reaction (PCR) was performed and cytokine mRNA expression for tumor necrosis factor alpha (TNFalpha), interleukin 1beta (IL-1beta), interleukin 10 (IL-10) and interferon gamma (IFN-gamma) determined using real time PCR. A significant effect of LPS stimulation over time was seen on TNFalpha, IL-1beta, IL-10 and IFN-gamma production. No significant difference was observed between the PGG-Glucan treatment group and control cells at any time point.
Sykes
Benjamin W
BW
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Helsinki, 00014 Helsinki, Finland.
Furr
Martin
M
Giguère
Steeve
S
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004334:Drug Administration Schedule; D004731:Endotoxins; D006736:Horses / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000378:metabolism*; D047071:beta-Glucans / Q000008:administration & dosage / Q000494:pharmacology*
D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D047071:beta-Glucans; C069894:poly-1-6-glucopyranosyl-1-3-glucopyranose glucan