Novel functional biodegradable polymer. III. The construction of poly(gamma-glutamic acid)-sulfonate hydrogel with fibroblast growth factor-2 activity.
Poly(gamma-glutamic acid)-sulfonate (gamma-PGA-S) hydrogel, with fibroblast growth factor (FGF)-2 activity was investigated as a novel, next-generation tissue-engineering material. gamma-PGA-net-gamma-PGA-S72 (S72-netgel) was prepared with gamma-PGA-S and gamma-PGA-S72 (72% sulfonated gamma-PGA) to provide the high mobility of gamma-PGA-S72 for FGF-2 activity. Cell adhesion and proliferation activities were evaluated on gamma-PGA and gamma-PGA-S hydrogels along with S72-netgels. Both cell adhesion and proliferation activities of gamma-PGA and gamma-PGA-S hydrogels were low. In contrast, S72-netgels had high cell adhesion and proliferation activities, because of their low swelling ratios and high sulfonic acid group concentrations. Furthermore, S72-netgels had high FGF-2 activity, because gamma-PGA-S72 retained FGF-2 activity when incorporated into S72-netgels. S72-netgels should be useful as next-generation tissue-engineering material containing FGF-2 activity.
Matsusaki
Michiya
M
Department of Nanostructured and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
Serizawa
Takeshi
T
Kishida
Akio
A
Akashi
Mitsuru
M
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels; D051379:Mice; D008956:Models, Chemical; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering
D001672:Biocompatible Materials; D020100:Hydrogels; D011108:Polymers; D013451:Sulfonic Acids; C451199:poly(glutamic acid)sulfonate; D016222:Fibroblast Growth Factor 2; D011099:Polyglutamic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate