Interactions between beta D372 and gamma subunit N-terminus residues gamma K9 and gamma S12 are important to catalytic activity catalyzed by Escherichia coli F1F0-ATP synthase.
Substitution of Escherichia coli F(1)F(0) ATP synthase residues betaD372 or gammaS12 with groups that are unable to form a hydrogen bond at this location decreased ATP synthase-dependent cell growth by 2 orders of magnitude, eliminated the ability of F(1)F(0) to catalyze ATPase-dependent proton pumping in inverted E. coli membranes, caused a 15-20% decrease in the coupling efficiency of the membranes as measured by the extent of succinate-dependent acridine orange fluorescence quenching, but increased soluble F(1)-ATPase activity by about 10%. Substitution of gammaK9 to eliminate the ability to form a salt bridge with betaD372 decreased soluble F(1)-ATPase activity and ATPase-driven proton pumping by 2-fold but had no effect on the proton gradient induced by addition of succinate. Mutations to eliminate the potential to form intersubunit hydrogen bonds and salt bridges between other less highly conserved residues on the gamma subunit N-terminus and the beta subunits had little effect on ATPase or ATP synthase activities. These results suggest that the betaD372-gammaK9 salt bridge contributes significantly to the rate-limiting step in ATP hydrolysis of soluble F(1) while the betaD372-gammaS12 hydrogen bond may serve as a component of an escapement mechanism for ATP synthesis in which alphabetagamma intersubunit interactions provide a means to make substrate binding a prerequisite of proton gradient-driven gamma subunit rotation.
Lowry
David S
DS
Center for the Study of Early Events in Photosynthesis, School of Life Sciences, Arizona State University, P.O. Box 874501, Tempe, Arizona 85287-4501, USA.
Frasch
Wayne D
WD
D000165:Acridine Orange / Q000737:chemistry; D000818:Animals; D001224:Aspartic Acid / Q000235:genetics; D002384:Catalysis; D002417:Cattle; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D005947:Glucose / Q000737:chemistry; D007700:Kinetics; D008239:Lysine / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary / Q000235:genetics; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics; D017494:Proton Pumps / Q000737:chemistry / Q000235:genetics; D012694:Serine / Q000235:genetics; D013050:Spectrometry, Fluorescence; D019802:Succinic Acid / Q000737:chemistry; D013816:Thermodynamics
D003470:Culture Media; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D021122:Protein Subunits; D017494:Proton Pumps; D001224:Aspartic Acid; D012694:Serine; D019802:Succinic Acid; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases; D000165:Acridine Orange; D005947:Glucose; D008239:Lysine