Suppressive effects of red wine polyphenols on voltage-gated ion channels in dorsal root ganglionic neuronal cells.
Polyphenols are ubiquitous plant metabolites with multiple pharmacological properties. Using whole-cell patch-clamp current recording techniques, we studied the effects of polypnenols extracted from red wine (purity > 90% from Cabernet Sauvignon grape wine) on the activities of voltage-operated Na+-, K+-, and Ca2+-channel currents in mouse dorsal root ganglionic neuronal cells. The polyphenols suppressed all of the channel activities with half-effective concentrations of about 2.5, 4.0, and 0.8-1.5 micro g/ml, respectively. In contrast, they showed no noticeable effects on the ion channels in other types of cells, including large conductance K+-channels in mouse lacrimal acinar cells. Thus, the polyphenols suppress firings of the action potential in the neuronal cells and could show a sedative effect on the excitation. We expect that red wine can be used as a remedy for excessive sensory stimuli.
Wu
Yan L
YL
Department of Physiology, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan.
Ohsaga
Atsushi
A
Oshiro
Takako
T
Iinuma
Kazuie
K
Kondo
Yoshiaki
Y
Ebihara
Satoru
S
Sasaki
Hidetada
H
Maruyama
Yoshio
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004553:Electric Conductivity; D004594:Electrophysiology; D005419:Flavonoids / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology*; D015640:Ion Channel Gating / Q000187:drug effects*; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014920:Wine
D005419:Flavonoids; D007473:Ion Channels; D010636:Phenols; D059808:Polyphenols; D012964:Sodium; D011188:Potassium; D002118:Calcium