In vivo and in vitro exposures of carp and carp tissues to graded concentrations of endocrine disrupting chemicals.
The objective of this study was to correlate morphological and biochemical parameters of reproductive activity in fish exposed to graded concentrations of endocrine disrupting chemicals. Two-year-old carps were exposed for two weeks to graded concentrations of ethynyl-estradiol, tamoxifen, and flutamide. For each chemical, morphological alterations of the gonads and liver were described according to biomarker and sex steroid levels. In parallel, isolated follicular cells (FCs) and testis fragments were incubated in the presence or absence of carp pituitary homogenate and 25-hydroxycholesterol. Media were analyzed for sex steroid level evaluations.
Bottero
S
S
DiBiSAA, University of Genoa, Italy. bottero@unige.it
Cevasco
A
A
Monteverde
M
M
Pedemonte
F
F
Bertolotti
R
R
Viganò
L
L
Mandich
A
A
Massari
A
A
D000818:Animals; D002347:Carps / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000008:administration & dosage* / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012098:Reproduction / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D052244:Endocrine Disruptors; D012739:Gonadal Steroid Hormones