Tethered phytic acid as a probe for measuring phytase activity.
A novel approach for measuring phytase activity is presented. We have developed a new chromophoric substrate analog of phytic acid, 5-O-[6-(benzoylamino)hexyl]-d-myo-inositol-1,2,3,4,6-pentakisphosphate that permits direct measurement of the phosphate ester bond-cleavage reaction using HPLC. This compound, along with its dephosphorylated T-phosphatidylinositol intermediates, are quantified using reversed phase chromatography with UV detection.
Berry
Duane F
DF
Virginia Polytechnic Institute and State University, Department of Crop and Soil Environmental Sciences, Blacksburg, VA 24061, USA. duberry@vt.edu
Berry
David A
DA
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D015335:Molecular Probes; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D010833:Phytic Acid / Q000032:analysis* / Q000378:metabolism; D013379:Substrate Specificity
D007295:Inositol Phosphates; D015335:Molecular Probes; D007294:Inositol; D010833:Phytic Acid; D010744:Phosphoric Monoester Hydrolases; C400321:3-phytase