title: Effect of media composition and in vitro activity of posaconazole, caspofungin and voriconazole against zygomycetes.
abstract: OBJECTIVE
  The effect of different media and composition on the in vitro activity of posaconazole, caspofungin and voriconazole against 59 zygomycetes species was determined.
  
  
  METHODS
  The media tested were RPMI 1640 medium with and without 2% glucose, antibiotic medium 3 (AM3) with and without 2% glucose, and high resolution (HR) medium.
  
  
  RESULTS
  Posaconazole was significantly more active than caspofungin and voriconazole, both in RPMI 1640 medium with 2% glucose and in HR medium. Adding glucose improved the determination of end points, but had only minor influence on the MICs. MICs evaluated in AM3 were lower than in RPMI 1640 medium or HR medium.
  
  
  CONCLUSIONS
  The in vivo effect of posaconazole in zygomycosis needs further evaluation.
authors.affiliation: Department of Paediatrics III, Paediatric Pulmonology and Infectious Diseases, University of Heidelberg, Im Neuenheimer Feld 153, D-69120 Heidelberg, Germany.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gil-Lamaignere
authors.forename: Regina
authors.initials: R
authors.lastname: Hess
authors.forename: Stefanie
authors.initials: S
authors.lastname: Salvenmoser
authors.forename: Kathrin
authors.initials: K
authors.lastname: Heyn
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kappe
authors.forename: Frank-Michael C
authors.initials: FM
authors.lastname: Müller
mesh_terms: D020095:Absidia / Q000187:drug effects / Q000254:growth & development; D000935:Antifungal Agents / Q000494:pharmacology*; D000077336:Caspofungin; D003470:Culture Media; D020093:Cunninghamella / Q000187:drug effects / Q000254:growth & development; D054714:Echinocandins; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests; D009089:Mucor / Q000187:drug effects / Q000254:growth & development; D009090:Mucorales / Q000187:drug effects* / Q000254:growth & development; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D012233:Rhizopus / Q000187:drug effects / Q000254:growth & development; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D054714:Echinocandins; D055666:Lipopeptides; D010456:Peptides, Cyclic; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D000077336:Caspofungin; D065819:Voriconazole