Candidate multi-peptide-vaccine against classical swine fever virus induced potent immunity with serological marker.
Our previous study proposed a protective multi-peptide-vaccine (MPV) with Freund's adjuvant against classical swine fever virus (CSFV). In this study, another candidate MPV, using aluminum adjuvant, was further examined. All immunized pigs kept healthy during the experimental period, while the control group rapidly showed clinical symptoms and died. Moreover, anti-sera from MPV-immunized pigs could interact with peptides involved in the MPV, in contrast to anti-sera from non-immunized or infected ones. This property permits MPV-immunized pigs to be easily differentiated from infected ones with simple serological method. Therefore, this new MPV is suitable to act as a candidate marker vaccine against CSFV.
Dong
Xiao-Nan
XN
Laboratory of Immunology, Department of Biology, Tsinghua University, Beijing 100084, PR China. xndong00@mails.tsinghua.edu.cn
Chen
Yu
Y
Wu
Yi
Y
Chen
Ying-Hua
YH
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006691:Classical Swine Fever / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007273:Injections, Intramuscular; D016019:Survival Analysis; D013552:Swine; D022581:Vaccines, Marker; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D022581:Vaccines, Marker; D022223:Vaccines, Subunit; D014765:Viral Vaccines; D000536:Aluminum Hydroxide