[Effect of baicalin on the proliferation of insulinoma cell line].
OBJECTIVE
To investigate the effect of baicalin on the proliferation of insulinoma cell line and the molecular mechanism involved.


METHODS
Such methods as light microscope, MTT assay, flow cytometry and Western blotting were applied to investigate the effects of baicalin (0, 100, 200, and 400 microg/ml baicalin treated for 24 h or 200 microg/ml baicalin treated at different time points) on the cell proliferation, cell survival rate, the cell cycle and related molecular mechanisms.


RESULTS
The number of proliferating cells obviously decreased with the increase of baicalin under the light microscope, and the survival rate of cells decreased as determined by MTT assay. After being treated with baicalin, the number of insulinoma cells in S-phase obviously decreased from 38.2% (0 microg/ml) to 9.4% (400 microg/ml), and the number of cells in phase G1 increased from 56.4% (0 microg/ml) to 85.9% (400 microg/ml). In the meantime, the expression of cyclin D1 was obviously declined by Western blotting.


CONCLUSIONS
Baica-lin can inhibit the proliferation of insulinoma cells, and the down-regulation of the expression of cyclin D1 might also be involved in these events.
Song
Jian-hui
JH
Department of Pediatrics, Xiangya Hospital , Central South University, Changsha 410008.
Sun
Ji-ping
JP
Jia
Yan-jie
YJ
Yang
Yu-jia
YJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007340:Insulinoma / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D019938:Cyclin D1; C038044:baicalin