title: Effect of glyphosate contaminated feed on rumen fermentation parameters and in sacco degradation of grass hay and corn grain.
abstract: Abstract Four rumen fistulated wethers were used to investigate the effect of glyphosate contaminated feed on rumen fermentation. The rations were based on corn silage, urea and a vitamin-mineral premix, either in the absence or presence of 0.77 g glyphosate per kg DM. Furthermore, rations were fed either with or without aromatic amino acid supplementation. During four periods of 28 days, sheep received each of the four dietary treatments according to a Latin square. After 14 days of adaptation rumen fermentation parameters (pH, ammonia, volatile fatty acids) were measured on day 15 over a five-hour period after the morning feeding. The remaining 13 days served for in sacco degradation studies with grass hay and corn grain. Ammonia (NH3) and pH of rumen fluid were within the normal range for all dietary treatments (NH3: 9.1-32.3 mmol x l(- l), pH: 6.2-6.7). Neither rumen fermentation parameters nor in sacco DM and NDF degradation of incubated feedstuffs were significantly affected by glyphosate, with or without aromatic amino acid supplementation. Kinetic profiles of the in sacco dry matter and NDF degradation of grass hay were almost identical for the dietary treatments.
authors.affiliation: Institute of Animal Nutrition, Federal Agricultural Research Centre (FAL), Braunschweig, Germany. liane.huether@fal.de
authors.forename: Liane
authors.initials: L
authors.lastname: Hüther
authors.forename: Svenja
authors.initials: S
authors.lastname: Drebes
authors.forename: Peter
authors.initials: P
authors.lastname: Lebzien
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D005506:Food Contamination; D005998:Glycine / Q000008:administration & dosage / Q000009:adverse effects* / Q000031:analogs & derivatives*; D006540:Herbicides / Q000008:administration & dosage / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration; D008297:Male; D010573:Pesticide Residues / Q000009:adverse effects / Q000032:analysis; D006109:Poaceae; D011897:Random Allocation; D012417:Rumen / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D003313:Zea mays; D000097797:Glyphosate
chemical_list: D005232:Fatty Acids, Volatile; D005998:Glycine; D006540:Herbicides; D010573:Pesticide Residues; D000097797:Glyphosate