title: Partial protection against Eimeria acervulina and Eimeria tenella induced by synthetic peptide vaccine.
abstract: Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella antigens) homogenized in Freund's complete and incomplete adjuvants. The immune responses to the vaccine were assessed by evaluation of antibody and lymphocyte proliferation responses, and the degree of resistance of vaccinated chickens to challenge with sporulated oocysts of E. acervulina or E. tenella determined by comparison of their oocyst output with those of control chickens. The results indicated that the synthetic peptide vaccine induced a high level of antibody and cellular responses associated with partial cross-species protection against challenge with sporulated oocysts of E. acervulina or E. tenella.
authors.affiliation: Poultry Diseases Division, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Iran. a_talebi1337@yahoo.com
authors.forename: Alireza
authors.initials: A
authors.lastname: Talebi
authors.forename: Grace
authors.initials: G
authors.lastname: Mulcahy
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D016786:Eimeria tenella / Q000276:immunology; D018604:Epitope Mapping / Q000662:veterinary; D007106:Immune Sera / Q000276:immunology; D007117:Immunization, Secondary / Q000662:veterinary; D008213:Lymphocyte Activation / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007106:Immune Sera; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic