title: Polymorphism of Aeromonas spp. tRNA intergenic spacers.
abstract: We investigated the length polymorphism of the intergenic spacers lying between tRNA genes of Aeromonas spp. A total of 69 strains representing all known genomic species of Aeromonas were used in the study. tDNA-PCR patterns were examined by Dice coefficient (S(D)) and unweighted pair group method of clustering (UPGMA). The strains were allocated into 15 groups at a similarity level of 70%. The strains belonging to seven genomic species: A. hydrophila (HG 1), A. caviae (HG 4), A. sobria (HG 7), A. veronii (HG 8/10), A. encheleia (HG 16), A. popoffii (HG 17), and A. culicicola (HG 18) formed distinct clusters. Our study revealed a genetic heterogeneity of the following species: A. bestiarum, A. salmonicida, A. media, A. eucrenophila, A. jandaei, A. schubertii, and A. allosaccharophila.
authors.affiliation: Institute of Experimental Biology, Department of Microbiology, Adam Mickiewicz University, ul. Fredry 10, 61-701 Poznań, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Laganowska
authors.forename: Adam
authors.initials: A
authors.lastname: Kaznowski
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics*; D005516:Food Microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D012329:RNA, Bacterial; D012343:RNA, Transfer