Nuclear localization of the major vault protein in U373 cells.
The major vault protein (MVP) is the predominant member of a large ribonucleoprotein particle, named vault. Vaults are abundant in the cytosol of mammalian cells. Mammalian MVP has previously been reported to be associated with the nucleus, particularly its cytosolic surface on which vaults are thought to dock at or near the nuclear pore complex. To date the presence of vault particles inside the nucleus has been convincingly reported only for sea urchin cells. We have addressed the potential nuclear localization of MVP in mammalian cells by employing confocal laser microscopy and cryo-immunoelectron microscopy. As revealed by immunostaining and by analysis of cells transfected with a construct encoding MVP and green fluorescent protein, MVP is present in both the cytosol and in the nucleus. Cryo-electron microscopy of human astroglioma U373 cells reveals clusters of immunogold particles at nuclear pores and in the nucleoplasm suggesting that nuclear MVP is associated with particulate structures. Quantification of the fluorescence observed in the cytosol and in the nuclei reveals that about 5% of the MVP in U373 cells is localized inside the nucleus. Our results further support the notion that part of the cellular MVP can enter the nucleus.
Slesina
Marco
M
Zoological Institute, AK Neurochemistry, Biocenter J.W. Goethe University, Marie-Curie-Strasse 9, 60439, Frankfurt am Main, Germany.
Inman
Elisabeth M
EM
Rome
Leonard H
LH
Volknandt
Walter
W
D001254:Astrocytoma / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D022022:Nuclear Pore / Q000378:metabolism / Q000648:ultrastructure; D014162:Transfection; D020394:Vault Ribonucleoprotein Particles / Q000378:metabolism* / Q000648:ultrastructure; D000099187:Major Vault Protein
D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein