Head space sensor array for the detection of aflatoxin M1 in raw ewe's milk.
A novel screening method was developed for simple and rapid detection of aflatoxin M1 contamination in raw ewe's milk samples without the need for sample pretreatment. The method was based on the use of a commercial head space sensor array system constituted by 12 metal oxide semiconductor sensors, 10 metal oxide semiconductor field-effect transistor sensors, and a pattern recognition software. Twenty-four raw milk samples collected from two different groups of ewes fed with a formulated feed that contained increasing amounts of aflatoxin B1 and six noncontaminated ewe's milk samples were analyzed. The results obtained by using the head space sensor array, processed by statistical methods, made it possible to group the samples according to the presence or the absence of aflatoxin M1. Sample classification was in complete agreement with the aflatoxin M1 content measured by an enzyme-linked immunosorbent assay procedure. This is the first report, to our knowledge, of detection of aflatoxin M1 in ewe's milk by a multisensor array.
Benedetti
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Dipartimento di Scienze e Tecnologie Alimentari, Università di Milano, Via Celoria, 2, 20133 Milano, Italy.
Iametti
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S
Bonomi
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F
Mannino
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D016607:Aflatoxin M1 / Q000302:isolation & purification*; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep
D016607:Aflatoxin M1