title: DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage.
abstract: Outside the magnetic field of the Earth, high energy heavy ions constitute a relevant part of the biologically significant dose to astronauts during the very long travels through space. The typical pattern of energy deposition in the matter by heavy ions on the microscopic scale is believed to produce spatially correlated damage in the DNA which is critical for radiobiological effects. We have investigated the influence of a lucite shielding on the initial production of very small DNA fragments in human fibroblasts irradiated with 1 GeV/u iron (Fe) ions. We also used gamma rays as reference radiation. Our results show: (1) a lower effect per incident ion when the shielding is used; (2) an higher DNA Double Strand Breaks (DSB) induction by Fe ions than by gamma rays in the size range 1-23 kbp; (3) a non-random DNA DSB induction by Fe ions.
authors.affiliation: Istituto Superiore di Sanità, Rome, Italy. francesca.antonelli@iss.it
authors.forename: F
authors.initials: F
authors.lastname: Antonelli
authors.forename: M
authors.initials: M
authors.lastname: Belli
authors.forename: A
authors.initials: A
authors.lastname: Campa
authors.forename: A
authors.initials: A
authors.lastname: Chatterjee
authors.forename: V
authors.initials: V
authors.lastname: Dini
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: B
authors.initials: B
authors.lastname: Rydberg
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.forename: M A
authors.initials: MA
authors.lastname: Tabocchini
mesh_terms: D002460:Cell Line; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D053938:DNA Fragmentation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005069:Evaluation Studies as Topic; D005118:Extraterrestrial Environment; D005347:Fibroblasts / Q000528:radiation effects; D005720:Gamma Rays; D020450:Heavy Ions; D006801:Humans; D007501:Iron; D018499:Linear Energy Transfer; D019904:Polymethyl Methacrylate; D011835:Radiation Protection / Q000295:instrumentation* / Q000379:methods; D012062:Relative Biological Effectiveness; D017356:Synchrotrons
chemical_list: D004247:DNA; D019904:Polymethyl Methacrylate; D007501:Iron