title: Synergistic effects of micropatterned biodegradable conduits and Schwann cells on sciatic nerve regeneration.
abstract: This paper describes a novel biodegradable conduit that provides a combination of physical, chemical and biological cues at the cellular level to facilitate peripheral nerve regeneration. The conduit consists of a porous poly(D,L-lactic acid) (PDLLA) tubular support structure with a micropatterned inner lumen. Schwann cells were pre-seeded into the lumen to provide additional trophic support. Conduits with micropatterned inner lumens pre-seeded with Schwann cells (MS) were fabricated and compared with three types of conduits used as controls: M (conduits with micropatterned inner lumens without pre-seeded Schwann cells), NS (conduits without micropatterned inner lumens pre-seeded with Schwann cells) and N (conduits without micropatterned inner lumens, without pre-seeded Schwann cells). The conduits were implanted in rats with 1 cm sciatic nerve transections and the regeneration and functional recovery were compared in the four different cases. The number or size of regenerated axons did not vary significantly among the different conduits. The time of recovery, and the sciatic function index, however, were significantly enhanced using the MS conduits, based on qualitative observations as well as quantitative measurements using walking track analysis. This demonstrates that biodegradable micropatterned conduits pre-seeded with Schwann cells that provide a combination of physical, chemical and biological guidance cues for regenerating axons at the cellular level offer a better alternative for repairing sciatic nerve transactions than conventional biodegradable conduits.
authors.affiliation: Chemical Engineering Department, University of Minnesota, 215 Engineering Building, Duluth, MN 55812-3025, USA.
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Rutkowski
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Miller
authors.forename: Srdija
authors.initials: S
authors.lastname: Jeftinija
authors.forename: Surya K
authors.initials: SK
authors.lastname: Mallapragada
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D009416:Nerve Regeneration / Q000502:physiology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D012583:Schwann Cells / Q000473:pathology / Q000637:transplantation*; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D020426:Sciatic Neuropathy / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)