Effect of environmental factors on production of lichenin, a chromosomally encoded bacteriocin-like compound produced by Bacillus licheniformis 26L-10/3RA.
Effect of environmental factors on production of lichenin, a bacteriocin-like compound produced by Bacillus licheniformis 26L-10/3RA isolated from buffalo rumen was studied. Lichenin represents the first anaerobiosis-specific expression of broad-spectrum antibacterial compound effective only under anaerobic conditions. Production of lichenin by B. licheniformis 26L-10/3RA was found to be very high at 39 degrees C in L-10 medium supplemented with 0.5% glucose and 20% (w/v) inert thermocol beads. Lichenin production was highest at pH 6.8 after 72-96h of incubation. Our study also indicated that Lichenin is not a plasmid-linked characteristic and is encoded by chromosomal DNA. Results obtained can be used in large-scale production of Lichenin for potential application in manipulating rumen function intended for improving productivity of the ruminants.
Pattnaik
Priyabrata
P
National Dairy Research Institute, Karnal-132 001, India. p.pat@rediffmail.com
Grover
Sunita
S
Batish
Virender Kumar
VK
D000693:Anaerobiosis; D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002020:Buffaloes; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005936:Glucans / Q000096:biosynthesis* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012417:Rumen / Q000382:microbiology; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D005936:Glucans; C015968:lichenin