[Gene reversion of induced differentiation of adult human bone marrow-derived mesenchymal stem cells into neuron-like cells].
OBJECTIVE
To observe gene reversion during differentiation of human adult bone marrow-derived mesenchymal stem cells (MSCs) into neuron-like cells induced by Shenqiye.


METHODS
The MSCs were separated, cultured and expanded in the culture medium and induced to differentiate into neuron-like cells with Shenqiye. The expressions of neuron-specific enolase (NSE), neurofilament (NF), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemical method, and the changes of 10 genes of the cells after differentiation were detected by reverse transcriptional PCR.


RESULTS
The MSCs exhibited neuronal phenotype when treated with Shenqiye and the neuron-like cells were positive for expressions of NSE and NF but not for glial astrocyte marker GFAP. After withdrawal of Shenqiye from the medium, the neuron-like cells were reversed to MSC, flat or spindal in morphology. The gene expression profiles of the redifferentiated cells were similar to those of undifferentiated MSCs.


CONCLUSIONS
Shenqiye can induce MSC differentiation into neuron-like cells during and after which gene reversion may occur.
Yao
Xiao-li
XL
Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Zhang
Cheng
C
Feng
Shan-wei
SW
Feng
Hui-yu
HY
Liu
Zu-guo
ZG
Deng
Yu-bin
YB
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016900:Neurofilament Proteins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology*; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase