Effect of ischemic preconditioning on reactive oxygen species-mediated ischemia--reperfusion injury in the isolated perfused rat lung.
OBJECTIVE
To investigate the protective effect of ischemic preconditioning (IP) in the early phase of reperfusion injury.


METHODS
Control rat lungs were subjected to 3 h of perfusion, whereas the lungs of the ischemia-then-reperfusion (I/R) group were subjected to 2 h of cold ischemia following 30 min of perfusion. IP was performed by two cycles of 5-min ischemia followed by 5 min of reperfusion prior to 2-h cold ischemia. Lipid peroxidation and antioxidant status were determined in tissue samples.


RESULTS
Lipid peroxidation and reduced/oxidized glutathione (GSH/GSSG) ratio were increased; antioxidant enzyme activities were decreased in the I/R group whereas lipid peroxidation and GSH/GSSG ratio were decreased; antioxidant enzyme activities were increased in the IP group.


CONCLUSIONS
IP appeared to have a protective effect against reactive oxygen species-mediated I/R injury in isolated rat lung.
Ucar
Gülberk
G
Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey. gulberk@hacepette.edu.tr
Topaloglu
Eda
E
Kandilci
H Burak
HB
Gümüsel
Bülent
B
D000818:Animals; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D019194:Ischemic Preconditioning; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D010544:Peroxidases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000503:physiopathology*
D017382:Reactive Oxygen Species; D010544:Peroxidases; D005978:Glutathione