[Os(bpy)2(CO)(enIA)][OTf]2: a novel sulfhydryl-specific metal-ligand complex.
The synthesis and physical-chemical characterization of the metal-ligand complex [Os(bpy)2(CO)(enIA)][OTf]2 (where enIA = ethylenediamine iodoacetamide) with a sulfhydryl-specific functional group is described. The UV and visible absorption and luminescence emission, including lifetime and steady-state anisotropy, are reported for the free probe and the probe covalently linked to two test proteins. The spectroscopic properties of the probe are unaffected by chemical modification and subsequent covalent linkage to the proteins. The luminescence lifetime in aqueous buffer is approximately 200 ns and the limiting anisotropy is greater than 0.125, suggesting a potentially useful probe for biophysical investigations.
Garino
Claudio
C
Dipartimento di Chimica IFM, Università di Torino, Via P. Giuria 7, 10125 Torino, Italy.
Ghiani
Simona
S
Gobetto
Roberto
R
Nervi
Carlo
C
Salassa
Luca
L
Ancarani
Valentina
V
Neyroz
Paolo
P
Franklin
Laurie
L
Alexander Ross
J B
JB
Seibert
Eleanore
E
D003545:Cysteine / Q000737:chemistry; D045647:DNA Glycosylases / Q000737:chemistry; D004563:Electrochemistry; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D008024:Ligands; D049449:Luminescence; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009992:Osmium / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
C501372:(Os(bpy)2(CO)(enIA))(OTf)2; D008024:Ligands; D009942:Organometallic Compounds; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D009992:Osmium; D045647:DNA Glycosylases; D003545:Cysteine