Identification of a transcriptionally active hVH-5 pseudogene on 10q22.2.
Mitogen-activated protein kinases (MAPKs) are important regulators of a vast number of biological functions that affect life and death of eukaryotic cells and are tightly regulated by the concerted action of several phosphatases. Among these is the human homologue of vaccinia virus H1 phosphatase gene clone 5 (hVH-5) product, which dephosphorylates and thus inhibits members of the MAPK family. Here, we analyzed hVH-5 transcripts in mammary carcinoma cell lines and discovered a sequence with 88% similarity to hVH-5 transcripts. Because this variant of hVH-5 lacked intronic sequences in its genomic structure, we suggest it might be a processed pseudogene of hVH-5. psihVH-5 transcripts were detected in human peripheral tissues as well as in 11 of 14 breast cancer cell lines. In respect to the normal hVH-5 gene, the pseudogene contains several point mutations and a frame shift due to the deletion of 2 bases that would lead to the truncation of the putative psihVH-5 product.
Berger
Ingemarie R
IR
Department of Molecular Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
Buschbeck
Marcus
M
Bange
Johannes
J
Ullrich
Axel
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D054637:Dual-Specificity Phosphatases; D006801:Humans; D009154:Mutation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011544:Pseudogenes; D016415:Sequence Alignment; D014018:Tissue Distribution; D014158:Transcription, Genetic
C517038:DUSP8 protein, human; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases