Occurrence of heavy metal-resistance in microflora from serpentine soil of Andaman.
Serpentine soils collected from Saddle Hills, Chidyatapu and Rutland of Andaman Islands, India were analyzed for physico-chemical and microbiological characteristics and compared with those from adjacent non-serpentine localities. The serpentine soils contained high levels of nickel (1740.0-8033.4 mg/kg dry soil), cobalt (93.2-533.4 mg/kg dry soil) and chromium (302.9-4437.0 mg/kg dry soil), in addition to 62-152 g of iron and 37-60 g of magnesium per kg dry soil. Characteristically the serpentine soils showed low microbial density (6.2-11.3 x 10(6) colony forming unit/g soil) and activity (1.7-3.5 microg fluorescein/g dry soil/h) than non-serpentine outcrops. Serpentine microbial population was dominated by bacteria which represented 5.12 to 9.5 x 10(6) cfu/g of soil, while the fungal population ranged from 0.17 to 3.21 x 10(6) cfu/g of soil. A total of 342 (200 from serpentine and 142 from non-serpentine soils) isolates were compared for Ni, Co and Cr resistance. Serpentine microflora was in general, highly resistant than non-serpentine ones and showed a metal-resistance profile of Cr > Ni > Co. Amongst the serpentine isolates, 8 and 11 bacteria tolerated > 12.0 mM Ni and > 16.0 mM Cr respectively, while 6 fungal isolates showed a minimum inhibitory concentration (MIC) value > 8.0 mM Co. These 25 serpentine strains also showed co-resistance to Cu, Zn and Mn but were sensitive to Hg and Cd. The selected bacterial isolates were resistant to ampicillin, penicillin G and polymyxin B, whereas fungal strains showed resistance to amphotericin B, nystatin and fusidic acid.
Pal
Arundhati
A
Microbiology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India.
Dutta
Suman
S
Mukherjee
P K
PK
Paul
A K
AK
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D017632:Asbestos, Serpentine / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D002857:Chromium / Q000032:analysis; D003035:Cobalt / Q000032:analysis; D024881:Drug Resistance, Bacterial; D025141:Drug Resistance, Fungal; D005658:Fungi / Q000187:drug effects* / Q000302:isolation & purification; D007194:India; D007501:Iron / Q000032:analysis; D008274:Magnesium / Q000032:analysis; D008670:Metals / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009532:Nickel / Q000032:analysis; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D017632:Asbestos, Serpentine; D008670:Metals; D012987:Soil; D002857:Chromium; D003035:Cobalt; D009532:Nickel; D007501:Iron; D008274:Magnesium