A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines.
OBJECTIVE
To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines.


RESULTS
A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and (1)H and (13)C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC(50) value of 0.65 and 5.07 Łg ml(-1) respectively.


CONCLUSIONS
Harzianum A with a chemical formula of C(23)H(28)O(6) was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines.


CONCLUSIONS
This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.
Lee
H B
HB
Department of Biological Sciences, Seoul National University, Seoul, Korea.
Kim
Y
Y
Jin
H Z
HZ
Lee
J J
JJ
Kim
C-J
CJ
Park
J Y
JY
Jung
H S
HS
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002845:Chromatography; D004271:DNA, Fungal / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004043:Dietary Fiber / Q000382:microbiology; D006801:Humans; D035901:Hypocrea / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014255:Trichothecenes / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*
D000970:Antineoplastic Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D004043:Dietary Fiber; D014255:Trichothecenes; C087546:harzianum A