title: Expression of Reg/PAP family members during motor nerve regeneration in rat.
abstract: In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.
authors.affiliation: Department of Anatomy and Neurobiology, Osaka City University, Graduate School of Medicine, Osaka 545-8585, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Namikawa
authors.forename: Munehisa
authors.initials: M
authors.lastname: Fukushima
authors.forename: Koichi
authors.initials: K
authors.lastname: Murakami
authors.forename: Akinobu
authors.initials: A
authors.lastname: Suzuki
authors.forename: Shin
authors.initials: S
authors.lastname: Takasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyama
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D007002:Hypoglossal Nerve / Q000473:pathology / Q000503:physiopathology*; D061228:Hypoglossal Nerve Injuries; D037181:Lectins, C-Type / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000378:metabolism* / Q000473:pathology; D009416:Nerve Regeneration / Q000502:physiology*; D000073718:Pancreatitis-Associated Proteins; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; C000612142:Reg2 protein, rat; C000612144:Reg3b protein, rat