Regulation of myeloid leukemia factor-1 interacting protein (MLF1IP) expression in glioblastoma.
The myelodysplasia/myeloid leukemia factor 1-interacting protein MLF1IP is a novel gene which encodes for a putative transcriptional repressor. It is localized to human chromosome 4q35.1 and is expressed in both the nuclei and cytoplasm of cells. Northern and Western blot analyses have revealed MLF1IP to be present at very low amounts in normal brain tissues, whereas a number of human and rat glioblastoma (GBM) cell lines demonstrated a high level expression of the MLF1IP protein. Immunohistochemical analysis of rat F98 and C6 GBM tumor models showed that MLF1IP was highly expressed in the tumor core where it was co-localized with MLF1 and nestin. Moreover, MLF1IP expression was elevated in the contralateral brain where no tumor cells were detected. These observations, together with previous data demonstrating a role for MLF1IP in erythroleukemias, suggest a possible function for this protein in glioma pathogenesis and potentially in other types of malignancies.
Hanissian
Silva H
SH
Department of Neurosurgery, The University of Tennessee Health Science Center, 847 Monroe, Memphis, TN 38163, USA. shanissi@utmem.edu
Teng
Bin
B
Akbar
Umar
U
Janjetovic
Zorica
Z
Zhou
Qihong
Q
Duntsch
Christopher
C
Robertson
Jon H
JH
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism* / Q000503:physiopathology; D006657:Histones; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009687:Nuclear Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology
C485917:CENPU protein, human; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006657:Histones; D007381:Intermediate Filament Proteins; C100852:MLF1 protein, human; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D009687:Nuclear Proteins; D011506:Proteins