Root responses and metal accumulation in two contrasting ecotypes of Sedum alfredii Hance under lead and zinc toxic stress.
Sedum alfredii Hance has been reported to be a Zn-hyperaccumulator plant species. In this study, root morphological and physiological response of the hyperaccumulating ecotype of S. alfredii H. (HE) from the mined area and the non-hyperaccumulating ecotype of S. alfredii H. (NHE) from the agricultural area to supplied levels of Zn and Pb were investigated. The results showed that Zn concentrations in the leaves and the stems of the HE were 34 and 41 times higher, whereas lead concentrations were 1.9 and 2.4 times greater, respectively, than those of the NHE when grown at 1224 microM Zn and/or 200 microM Pb. At combined supply of 1224 microM Zn with 200 microM Pb, however, zinc concentrations in the stems and leaves of the, HE decreased, while lead concentrations in the stems increased significantly, as compared with those of single metal treatment. Lead uptake of the HE was enhanced by Zn addition. Root activity of the HE decreased by Pb treatment in the first two days, but recovered afterward and close to the control at day 10 of the treatment. However, root activity of the NHE decreased by each metal treatment, and was not recovered with the advance of treatment time. Root length, root surface area, and root volumes increased obviously due to Zn and/or Pb/Zn combined treatments for the HE, but significantly decreased due to Pb, Zn, or Pb/Zn combined treatment for the NHE. Zinc and Pb concentrations in both ecotypes of S. alfredii H. were positively correlated with root length, root surface area, and root volumes. Root exudates of the HE, especially treated with Zn, increased the extractability of Pb and Zn from the mined soil. At the Zn supply level of 1224microM, the extractability of root exudates on soil Pb was 3-12 times greater for the HE than for the NHE. These results imply that the tolerance and hyperaccumulation of the hyperaccumulating ecotype of S. alfredii H. to Zn and Pb appear to be closely related to its high adaptation of root growth, morphology, and physiology to Pb and Zn toxicity, and through its root excretion of some special substances that can activate Pb and Zn in the mined soil, thus increasing their mobilization and bioavailability.
Li
Ting-Qiang
TQ
MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Natural Resources and Environmental Science, Zhejiang University, Hangzhou, China.
Yang
Xiao-E
XE
Jin
Xiao-Fen
XF
He
Zhen-Li
ZL
Stoffella
Peter-J
PJ
Hu
Qing-Hua
QH
D017753:Ecosystem; D018527:Hydroponics; D007854:Lead / Q000032:analysis* / Q000493:pharmacokinetics; D008906:Mining; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*; D031963:Sedum / Q000254:growth & development* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis* / Q000493:pharmacokinetics; D015032:Zinc / Q000032:analysis* / Q000493:pharmacokinetics
D012989:Soil Pollutants; D007854:Lead; D015032:Zinc