Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus.
The reconstructed cellular metabolic network of Mus musculus, based on annotated genomic data, pathway databases, and currently available biochemical and physiological information, is presented. Although incomplete, it represents the first attempt to collect and characterize the metabolic network of a mammalian cell on the basis of genomic data. The reaction network is generic in nature and attempts to capture the carbon, energy, and nitrogen metabolism of the cell. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, including transport reactions between the compartments and the extracellular medium. The reaction list consists of 872 internal metabolites involved in a total of 1220 reactions, whereof 473 relate to known open reading frames. Initial in silico analysis of the reconstructed model is presented.
Sheikh
Kashif
K
Department of Chemical Engineering, The University of Queensland, St. Lucia, 4072 QLD, Australia, and Fluxome Sciences A/S, Søltofts Plads, Building 223, 2800 Lyngby, Denmark.
Förster
Jochen
J
Nielsen
Lars K
LK
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019992:Databases as Topic; D023281:Genomics / Q000379:methods*; D005947:Glucose / Q000494:pharmacology; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D008954:Models, Biological; D008957:Models, Genetic
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D005947:Glucose