title: Systemic soluble Tie2 expression inhibits and regresses corneal neovascularization.
abstract: This study was designed to determine if soluble Tie2 (sTie2) expression inhibits and regresses corneal neovascularization, and if VEGF contributes to its effect. The corneas of BALB/c mice were scraped and the mice were injected with either an adenovirus expressing soluble Tie2 (Ad.sTie2) or an empty adenoviral vector. When injected at the inhibition timepoint (one day prior to corneal injury), the mean percentage of neovascularized corneal area two weeks later in Ad.sTie2-treated mice vs. controls was 56.37+/-9.15% vs. 85.79+/-3.55% (p=0.04). At the regression timepoint (4 weeks after corneal scrape), the mean area of corneal neovascularization in Ad.sTie2-treated mice was 42.89+/-4.74% vs. 75.01+/-3.22% in the control group (p=0.007). VEGF expression was significantly higher in Ad.sTie2-treated mice at the inhibition timepoint and there was no significant difference at the regression timepoint. These findings suggest that sTie2 inhibits and regresses corneal neovascularization in a VEGF-independent manner.
authors.affiliation: Department of Ophthalmology, Medical College of Georgia, Augusta, GA, USA.
authors.forename: Nirbhai
authors.initials: N
authors.lastname: Singh
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Macnamara
authors.forename: Saadia
authors.initials: S
authors.lastname: Rashid
authors.forename: Jayakrishna
authors.initials: J
authors.lastname: Ambati
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Kontos
authors.forename: Eric
authors.initials: E
authors.lastname: Higgins
authors.forename: Balamurali K
authors.initials: BK
authors.lastname: Ambati
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D016510:Corneal Neovascularization / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D008571:Men; D051379:Mice; D008807:Mice, Inbred BALB C; D042787:Receptor, TIE-2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D014162:Transfection / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D042787:Receptor, TIE-2