Reduced endothelial secretion and plasma levels of transforming growth factor-beta1 in patients with hereditary hemorrhagic telangiectasia type 1.
OBJECTIVE
To determine if patients with hereditary hemorrhagic telangiectasia (HHT) show alterations in transforming growth factor (TGF)-beta and its pathways.


METHODS
Blood samples were obtained from HHT patients and controls, while endothelial cells were derived from umbilical veins of newborns (HUVEC) from HHT families. TGF-beta1 in plasma, or secreted by HUVEC, and plasma endoglin levels were measured by ELISA. Cellular levels of endoglin and receptor Smad proteins were tested by metabolic labeling and immunoprecipitation, mRNA levels for endoglin and TGF-beta1 by real-time PCR, and receptor Smad phosphorylation by Western blotting.


RESULTS
TGF-beta1 and endoglin plasma levels analyzed in 197 individuals showed an inverse correlation with age. Circulating levels of TGF-beta1 were reduced in HHT1 patients (with Endoglin mutations) compared to control, but not in HHT2 patients (with ALK1 mutations). Endoglin levels were unchanged in plasma but decreased in activated monocytes and HUVEC with an HHT1 genotype. These HUVEC also expressed reduced levels of endoglin and TGF-beta1 mRNA, secreted less TGF-beta1, and showed normal receptor Smad expression and phosphorylation.


CONCLUSIONS
Decreased plasma TGF-beta1 levels in HHT1 patients correlate with reduced production by endothelial cells. The lower endoglin expression in these cells may alter the regulation of TGF-beta1 via Smad-independent pathways.
Letarte
Michelle
M
Cancer Research Program, Hospital for Sick Children, 555 University Avenue, Toronto, Canada, M5G 1X8. mablab@sickkids.ca
McDonald
Merry-Lynn
ML
Li
Chenggang
C
Kathirkamathamby
Kirishanthy
K
Vera
Sonia
S
Pece-Barbara
Nadia
N
Kumar
Shant
S
D015703:Antigens, CD; D016022:Case-Control Studies; D002478:Cells, Cultured; D000071063:Endoglin; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D007231:Infant, Newborn; D009154:Mutation; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051896:Smad Proteins, Receptor-Regulated / Q000032:analysis / Q000235:genetics; D018709:Statistics, Nonparametric; D013683:Telangiectasia, Hereditary Hemorrhagic / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood / Q000235:genetics
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051896:Smad Proteins, Receptor-Regulated; D016212:Transforming Growth Factor beta; D019010:Vascular Cell Adhesion Molecule-1