Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene.
Transgenic mouse lines were generated that express the Cre recombinase under the control of the distal promoter of the mouse Lck gene. Cre recombination in four of these lines of transgenic mice was characterized at the single cell level using ROSA26-regulated loxP-Stop-loxP-betageo and loxP-Stop-loxP-YFP reporter mouse lines. Two of the lines showed T cell-restricted Cre recombination, whereas the other two also expressed Cre in B cells, NK cells, and monocytes. Cre recombination began at a late stage of T cell development (at or after up-regulation of the TCR during positive selection) in the two T cell-restricted lines. Lines of mice that express the Cre recombinase at late stages of thymocyte development are of value for determining the impact of mutations on T cell function in the absence of complicating effects on early thymocyte selection.
Zhang
Dong Ji
DJ
Department of Microbiology and Immunology, University of California, San Francisco, 94143, USA.
Wang
Qi
Q
Wei
Jie
J
Baimukanova
Gyulnar
G
Buchholz
Frank
F
Stewart
A Francis
AF
Mao
Xiaohong
X
Killeen
Nigel
N
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D017930:Genes, Reporter; D019426:Integrases / Q000096:biosynthesis* / Q000235:genetics*; D007694:Killer Cells, Natural / Q000201:enzymology / Q000276:immunology; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000276:immunology*; D011995:Recombination, Genetic; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D019076:Transgenes
D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); C045073:Cre recombinase; D019426:Integrases