title: Determination of mechanism of flock sediment formation in tea beverages.
abstract: The mechanism of sediment formation during the storage of green tea beverage was investigated. Green tea extract was separated by Diaion HP-20 column chromatography, and a sediment-formation test was performed. Results showed that at least one compound of the substance causing flock sediment was contained in each of the HP-20 nonadsorbed and adsorbed fractions. From the following fractionations and structure analyses, the substance in the HP-20 adsorbed fraction was determined to be 1-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-beta-D-glucose (strictinin), which is one of the ellagitannins. Strictinin was hydrolyzed to ellagic acid by heat-sterilization processes such as retort sterilization or the ultra-high temperature processing used during the manufacturing of tea beverages. Ellagic acid combined with proteins in the HP-20 nonadsorbed fraction to form an irreversible sediment of green tea beverage; ellagic acid and proteins were confirmed to be present in that sediment. The HP-20 adsorbed fraction contained little strictinin and formed hardly any sediment, suggesting that control of the strictinin content is significant in avoiding sediment formation during the manufacturing process of tea beverages.
authors.affiliation: Central Research Institute and Quality Control, ITO EN Ltd., 21 Mekami, Sagara-cho, Haibara-gun, Shizuoka, 421-0516, Japan. h-nino@itoen.com.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niino
authors.forename: Iwao
authors.initials: I
authors.lastname: Sakane
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okanoya
authors.forename: Syuhei
authors.initials: S
authors.lastname: Kuribayashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kinugasa
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000032:analysis / Q000737:chemistry; D006358:Hot Temperature; D006868:Hydrolysis; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
chemical_list: D010636:Phenols; D013662:Tea; C496786:strictinin; D004610:Ellagic Acid