An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus.
Ryegrass mosaic virus (RGMV) is considered the most serious and widespread virus infecting temperate pasture grasses. The use of visible symptoms to diagnose infection is unreliable and ELISA analysis requires antibodies with broad cross-reactivity. Here we describe the production of a polyclonal antiserum (PAb-cp3'Delta) using a bacterially expressed RGMV coat protein fragment. The PAb-cp3'Delta antiserum is specific for RGMV and recognises RGMV strains from each major phylogenetic cluster. PAb-cp3'Delta may be used in ELISAs for fast, accurate and inexpensive detection of RGMV.
Webster
D E
DE
Botany Department, University of Otago, Dunedin, New Zealand. diane@burnet.edu.au
Beck
D L
DL
Rabenstein
F
F
Forster
R L S
RL
Guy
P L
PL
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001419:Bacteria / Q000378:metabolism; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007106:Immune Sera / Q000276:immunology*; D008129:Lolium / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera