title: Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis).
abstract: We recently reported the occurrence of natural infection with H. pylori in a group of cynomolgus monkeys with chronic active gastritis and gastric erosions. The goal of the present study was to characterize and to compare strains isolated from animals originating from two different geographical areas. Gross and microscopic pathology determined at the time of necropsy was similar in all animals. H. pylori were isolated from specimens harvested in five monkeys (four from Vietnam and one from the Philippines) with gastritis. Isolates from monkeys bred in Vietnam had a similar DNA fingerprint pattern, which was distinct from that of isolates from a monkey bred in the Philippines. All strains were of the s1a vacA subtype, but all the 'Vietnamese' strains were cagA+ and all but one were iceA1 whereas the 'Philippino' strains were cagA- and iceA2. The sequences of the 16S rRNA of the Vietnamese and Philippino strains shared 98% homology and both clustered with H. pylori sequences present in the NCBI database. In conclusion, cynomolgus monkeys can be naturally colonized by H. pylori, and the strains isolated from these animals appear to vary according to the geographical origin, thus indicating probable infection prior to importation. Since some of the cynomolgus monkeys developed antral erosions during natural infection, we propose that this animal model may be used to investigate the role of H. pylori in ulcerogenesis.
authors.affiliation: Department of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Room A3075, Bethesda, MD 20814, USA.
authors.forename: Sonia Q
authors.initials: SQ
authors.lastname: Doi
authors.forename: Tara
authors.initials: T
authors.lastname: Kimbason
authors.forename: James
authors.initials: J
authors.lastname: Reindel
authors.forename: Andre
authors.initials: A
authors.lastname: Dubois
mesh_terms: D000818:Animals; D016172:DNA Fingerprinting; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D008252:Macaca fascicularis / Q000382:microbiology*; D008992:Monkey Diseases / Q000382:microbiology*; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S