title: Serum IgG mediates mucosal immunity against rotavirus infection.
abstract: We evaluated the protective role of passively transferred circulating antibodies in protecting non-human primates against experimental rotavirus infection. Pooled sera with rotavirus-specific IgG titers that were either high (1:10,000), intermediate (1:300), or negative (< 1:25) were infused i.v. into naive pigtailed macaques (ages 3-6 months). Rotavirus-specific IgG could be detected in the sera at 18 h in all animals infused with antibody-containing serum, and fecal IgG titers could be detected only in animals given high-titer pooled sera. When orally challenged with 10(6) fluorescent-forming units of a simian rotavirus strain, YK-1, at 18 h after serum transfer, control animals shed virus starting 1-3 days after challenge and continued to shed virus at high titers for 6-8 days, whereas passively immunized macaques did not shed virus or had delayed shedding at low titers for only a limited time. The observation that passively transferred antibodies can suppress or delay viral infection in rotavirus-challenged pigtailed macaques has important implications for the design and testing of parenteral candidate rotavirus vaccines.
authors.affiliation: Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Viruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. lew2@cdc.gov
authors.forename: Larry E
authors.initials: LE
authors.lastname: Westerman
authors.forename: Harold M
authors.initials: HM
authors.lastname: McClure
authors.forename: Baoming
authors.initials: B
authors.lastname: Jiang
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Almond
authors.forename: Roger I
authors.initials: RI
authors.lastname: Glass
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D005243:Feces / Q000737:chemistry; D018928:Immunity, Mucosal / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008254:Macaca nemestrina; D009500:Neutralization Tests; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*; D014611:Vaccination; D017201:Virus Shedding / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G