title: Chromosomal aberrations induced by high-energy iron ions with shielding.
abstract: Biophysical models are commonly used to evaluate the effectiveness of shielding in reducing the biological damage caused by cosmic radiation in space flights. To improve and validate these codes biophysical experiments are needed. We have measured the induction of chromosomal aberrations in human peripheral blood lymphocytes exposed in vitro to 500 MeV/n iron ion beams (dose range 0.1-1 Gy) after traversing shields of different material (lucite, aluminium, or lead) and thickness (0-11.3 g/cm2). For comparison, cells were exposed to 200 MeV/n iron ions and to X-rays. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid cell-cycle selection produced by the exposure to high-LET heavy-ion beams. Aberrations were scored in chromosomes 1, 2, and 4 following fluorescence in situ hybridization. The fraction of aberrant lymphocytes has been evaluated as a function of the dose at the sample position, and of the fluence of primary 56Fe ions hitting the shield. The influence of shield thickness on the action cross-section for the induction of exchange-type aberrations has been analyzed, and the dose average-LET measured as a function of the shield thickness. These preliminary results prove that the effectiveness of heavy ions is modified by shielding, and the biological damage is dependent upon shield thickness and material.
authors.affiliation: Department of Physical Sciences, University of Naples Federico II and INFN, Napoli, Italy. gianfranco.grossi@na.infn.it
authors.forename: G
authors.initials: G
authors.lastname: Grossi
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: G
authors.initials: G
authors.lastname: Gialanella
authors.forename: M
authors.initials: M
authors.lastname: Pugliese
authors.forename: P
authors.initials: P
authors.lastname: Scampoli
authors.forename: Y
authors.initials: Y
authors.lastname: Furusawa
authors.forename: T
authors.initials: T
authors.lastname: Kanai
authors.forename: N
authors.initials: N
authors.lastname: Matsufuji
mesh_terms: D000535:Aluminum; D002453:Cell Cycle / Q000528:radiation effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004791:Enzyme Inhibitors; D006801:Humans; D007501:Iron; D007854:Lead; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000528:radiation effects; D008387:Marine Toxins; D010080:Oxazoles; D010315:Particle Accelerators; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D019904:Polymethyl Methacrylate; D011835:Radiation Protection; D013026:Space Flight
chemical_list: D004791:Enzyme Inhibitors; D008387:Marine Toxins; D010080:Oxazoles; D007854:Lead; C059041:calyculin A; D019904:Polymethyl Methacrylate; D000535:Aluminum; D007501:Iron; D010749:Phosphoprotein Phosphatases