title: Comparison of the four mouse fasciclin-containing genes expression patterns during valvuloseptal morphogenesis.
abstract: All four mammalian fasciclin-containing genes are expressed in the adult valves and are localized in partially overlapping and reciprocal patterns during cardiovascular development. Spatiotemporal comparison of the fasciclin-containing secreted adhesion genes, TGFbeta induced clone H3 (betaigH3) and periostin, revealed that they are co-localized within the outflow tract endocardial cushions, but that betaigH3 expression is restricted to the septal cushions within the atrioventricular canal. Conversely, the fasciclin-containing transmembrane gene, stabilin-1, is predominately expressed in the endocardial layer overlaying the cushions and lining the developing heart. However, expression of the fasciclin-containing transmembrane gene, stabilin-2 is only present in the post-natal mature valve endothelial cells. These data illustrate for the first time that the primitive endocardial cushions dynamically express multiple fasciclin-containing adhesion molecules as they undergo the key steps of seeding, proliferation, differentiation, fusion, mesenchymal condensation and remodeling during mouse heart development.
authors.affiliation: Cardiovascular Development Group, Herman B Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, 46202, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lindsley
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Nana
authors.initials: N
authors.lastname: Maeda
authors.forename: Rhonda
authors.initials: R
authors.lastname: Rogers
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Conway
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis* / Q000235:genetics; D018076:DNA, Complementary / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006346:Heart Septum / Q000196:embryology*; D006351:Heart Valves / Q000196:embryology* / Q000473:pathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D000099222:betaIG-H3 Protein
chemical_list: D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C082838:Postn protein, mouse; C486732:Stab1 protein, mouse; C486733:Stab2 protein, mouse