Adenovirus type 7 peptide diversity during outbreak, Korea, 1995-2000.
To understand the molecular basis of observed regional shifts in the genome types of adenovirus type 7 (Ad7) isolated in Korea during nationwide outbreaks from 1995 to 2000, the genetic variabilities of Ad7d and Ad7l were studied by sequence analysis of hexon, fiber, E3, and E4 open reading frame (ORF) 6/7 peptides. One amino acid change in the receptor-binding domain of fiber and 6 amino acid variations in E4 ORF 6/7 were identified between 2 genome types, while no variations were found in hexon and E3. Phylogenetic trees based on hexon, fiber, and E4 suggested that the Ad7 epidemic was probably caused by the introduction of the Japanese Ad7d strains. Our data also provide evidence that the rapid divergence of Ad7d to a novel genome type Ad7l could have been due to viral strategies involving multiple sequence changes in E4. This result suggests fiber and E4 ORF 6/7 peptides participate in the evolution of Ad7.
Choi
Eun Hwa
EH
Seoul National University College of Medicine, Seoul, Korea.
Kim
Hee Sup
HS
Eun
Byung Wook
BW
Kim
Beyong Il
BI
Choi
Jung Yeon
JY
Lee
Hoan Jong
HJ
Inada
Toshiki
T
D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics*; D004196:Disease Outbreaks; D014644:Genetic Variation; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D015183:Restriction Mapping