CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules.
CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8 kb on chromosome 16q22.1. Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis.
Shi
Shuang
S
Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, 38 Xueyuan Road, Beijing 100083, China.
Rui
Min
M
Han
Wenling
W
Wang
Ying
Y
Qiu
Xiaoyan
X
Ding
Peiguo
P
Zhang
Pei
P
Zhu
Xiaohui
X
Zhang
Yingmei
Y
Gan
Qini
Q
Ma
Dalong
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D018925:Chemokines / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D018076:DNA, Complementary; D006801:Humans; D007150:Immunohistochemistry; D062792:MARVEL Domain-Containing Proteins; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
C505071:CMTM2 protein, human; D018925:Chemokines; D018076:DNA, Complementary; D062792:MARVEL Domain-Containing Proteins