ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3.
Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3zeta and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.
Zhu
Pengcheng
P
The State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. pzhu@cbr.med.harvard.edu
Sang
Yingying
Y
Xu
Huanbing
H
Zhao
Jing
J
Xu
Rener
R
Sun
Yubo
Y
Xu
Tianhong
T
Wang
Xiaolei
X
Chen
Linfeng
L
Feng
Hanping
H
Li
Changben
C
Zhao
Shouyuan
S
D048948:14-3-3 Proteins / Q000502:physiology*; D051722:ADAM Proteins; D001665:Binding Sites; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D051722:ADAM Proteins; C498474:ADAM22 protein, human