Passive adsorption of human antirrabic immunoglobulin onto a polystyrene surface.
The latex agglutination immunoassay technique uses polymer colloids as carriers of adsorbed proteins to enhance the antigen-antibody reaction. The aim of the present work is to study the adsorption of Human Antirrabic Immunoglobulin (HA-IgG) on polystyrene latex (PS). The physical adsorption of HA-IgG on PS latex was investigated as a function of pH at 2 mM ionic strength. The amount of HA-IgG adsorbed onto PS latex greatly depends on pH; its value showed a maximum in the neighborhood of the IEP of HA-IgG. The electrophoretic method was applied to characterize latex particles. The influence of the amount of HA-IgG adsorbed (J(ads)) on the electrophoretic mobility and zeta-potential values was also studied.
Rosado
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E
Department of Macromolecular Chemistry, Biomaterials Center BIOMAT, University of Havana, P.O. Box 6130, Postal Code 10600, Havana, Cuba. rosado@biomat.uh.cu
Caroll
H
H
Sánchez
O
O
Peniche
C
C
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D004563:Electrochemistry; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry*; D007477:Ions; D007525:Isoelectric Focusing; D007700:Kinetics; D007840:Latex / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007477:Ions; D007840:Latex; D011108:Polymers; D011137:Polystyrenes