title: Abrogation of bronchial eosinophilic inflammation and attenuated eotaxin content in protease-activated receptor 2-deficient mice.
abstract: Protease-activated receptor 2 (PAR2) belongs to the PAR family (PAR1 to PAR4), which is activated by serine proteases (trypsin, tryptase, etc.). In this study, we evaluated the role of PAR2 in allergic inflammation of airways using PAR2-deficient (PAR2(-/-)) mice. In wild- type mice, infiltration of eosinophils and high eotaxin content were found in bronchoalveolar lavage fluid (BALF) after ovalbumin (OA) sensitization and following challenge. In contrast, both OA-induced infiltration of eosinophils and increase of eotaxin content were abrogated in BALF from PAR2(-/-) mice. The activation of PAR2 might be essential in the production of eotaxin and consequential allergic inflammation in airways.
authors.affiliation: Tokyo New Drug Research Laboratories II, Pharmaceutical Division, Kowa Company Ltd., Tokyo 189-0022, Japan. ttakizaw@kowa.co.jp
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takizawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Tamiya
authors.forename: Takuya
authors.initials: T
authors.lastname: Hara
authors.forename: Jiro
authors.initials: J
authors.lastname: Matsumoto
authors.forename: Naohiro
authors.initials: N
authors.lastname: Saito
authors.forename: Toru
authors.initials: T
authors.lastname: Kanke
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawagoe
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hattori
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000235:genetics; D004804:Eosinophils / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D044464:Receptor, PAR-2 / Q000172:deficiency* / Q000235:genetics
chemical_list: C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D044464:Receptor, PAR-2