Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum.
A Gram-negative, rod-shaped, non-spore-forming bacterium was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, LMG 22862(T) was shown to belong to the family Sphingobacteriaceae, related to Pedobacter africanus (98.0 %) and Pedobacter heparinus (97.6 %). The results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, SDS-PAGE, analysis of the fatty acid composition and physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22862(T) from Pedobacter species with validly published names. LMG 22862(T) therefore represents a novel species within this genus, for which the name Pedobacter caeni sp. nov. is proposed, with the type strain LMG 22862(T) (=DSM 16990(T)).
Vanparys
Bram
B
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Heylen
Kim
K
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Lebbe
Liesbeth
L
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
De Vos
Paul
P
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D020543:Proteome; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet; D009584:Nitrogen