title: Fra-1 activity in the frog, Rana esculenta, testis.
abstract: Using an anti-Fos family member antiserum, we previously described, in the testis of Rana esculenta, the presence of a nuclear 43-kDa protein that we hypothesized to be Fra-1. Using an antiserum against Fra-1, we here report on Fra-1 expression, localization, and putative activity in the R. esculenta testis during the annual reproductive cycle. Western blot analysis confirms that the nuclear 43-kDa protein is Fra-1. Immunocytochemistry demonstrates Fra-1 in peritubular myoid cells (PMC), efferent ducts, and blood vessels. We present, for the first time for a vertebrate, experimental evidence that the expression of Fra-1 in PMC is related to its activity during sperm transport from the tubular compartment to the efferent ducts.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sez. F. Bottazzi, II Università di Napoli, Naples, Italy.
authors.forename: Gilda
authors.initials: G
authors.lastname: Cobellis
authors.forename: Monica
authors.initials: M
authors.lastname: Lombardi
authors.forename: Donatella
authors.initials: D
authors.lastname: Scarpa
authors.forename: Gaia
authors.initials: G
authors.lastname: Izzo
authors.forename: Giulia
authors.initials: G
authors.lastname: Fienga
authors.forename: Rosaria
authors.initials: R
authors.lastname: Meccariello
authors.forename: Riccardo
authors.initials: R
authors.lastname: Pierantoni
authors.forename: Silvia
authors.initials: S
authors.lastname: Fasano
mesh_terms: D000818:Animals; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis*; D011893:Rana esculenta / Q000378:metabolism*; D013737:Testis / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D000099169:Fos-Related Antigen 1
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D000099169:Fos-Related Antigen 1