The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells.
Advances in tissue engineering require biofunctional scaffolds that can not only provide cells with structural support, but also interact with cells in a biological manner. To achieve this goal, a frequently used cell adhesion peptide Arg-Gly-Asp (RGD) was covalently incorporated into poly(ethylene glycol) diacrylate (PEODA) hydrogel and its dosage effect (0.025, 1.25 and 2.5 mm) on osteogenesis of marrow stromal cells in a three-dimensional environment was examined. Expression of bone-related markers, osteocalcin (OCN) and Alkaline phosphatase (ALP), increased significantly as the RGD concentration increased. Compared with no RGD, 2.5 mm RGD group showed a 1344% increase in ALP production and a 277% increase in OCN accumulation in the medium. RGD helped MSCs maintain cbfa-1 expression when shifted from a two-dimensional environment to a three-dimensional environment. Soluble RGD was found to completely block the mineralization of marrow stromal cells, as manifested by quantitative calcium assay, phosphorus elemental analysis and Von Kossa staining. In conclusion, we have demonstrated that RGD-conjugated PEODA hydrogel promotes the osteogenesis of MSCs in a dosage-dependent manner, with 2.5 mm being optimal concentration.
Yang
Fan
F
Department of Biomedical Engineering, Johns Hopkins University, 3400 North Charles Street/Clark Hall 106, Baltimore, MD 21218, USA.
Williams
Christopher G
CG
Wang
Dong-An
DA
Lee
Hyukjin
H
Manson
Paul N
PN
Elisseeff
Jennifer
J
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D004247:DNA / Q000737:chemistry; D006041:Goats; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D015233:Models, Statistical; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D015675:Osteocalcin / Q000737:chemistry / Q000378:metabolism; D010012:Osteogenesis; D010455:Peptides / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D020100:Hydrogels; D009842:Oligopeptides; D010455:Peptides; C437167:poly(ethylene glycol)diacrylate; D015675:Osteocalcin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010758:Phosphorus; D011092:Polyethylene Glycols; D012313:RNA; C047981:arginyl-glycyl-aspartic acid; D004247:DNA; D000469:Alkaline Phosphatase