title: Successful transplantation of hepatocytes requires temporary elimination of scavenger and NK cells, partial hepatectomy and ligation of bile duct.
abstract: Isolated syngeneic and allogeneic hepatocytes transplanted to spleen parenchyma are rapidly disintegrated. Host scavenger monocytes and macrophages are responsible for this reaction. We designed a method overcoming early disintegration of the intra-splenic grafted hepatocytes. It consisted of administration of anti-asialoGM I antiserum eliminating NK cells, sublethal whole body irradiation for depletion of scavenging cells and reconstitution with syngeneic bone marrow cells, ligation of host bile duct, intrasplenic hepatocyte transplantation and 3 consecutive partial hepatectomies. Six months after transplantation a glycogen-rich, trabeculae-forming, dividing hepatocytes, situated along strands of newly-formed fibrous tissue and numerous dilated blind bile canaliculae were observed. There was evidently more bile canaliculae in hosts with ligated bile duct than non-ligated controls. This is the first study showing fibrous tissue formed at the site of hepatocyte implantation and stellate cells are presumably responsible for this process.
authors.affiliation: Department of Surgical Research and Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wesolowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gewartowska
authors.forename: Waldemar L
authors.initials: WL
authors.lastname: Olszewski
authors.forename: Marek
authors.initials: M
authors.lastname: Durlik
mesh_terms: D000818:Animals; D001648:Bile Canaliculi / Q000166:cytology / Q000502:physiology*; D001652:Bile Ducts / Q000601:surgery*; D006003:Glycogen / Q000378:metabolism; D006085:Graft Survival; D006498:Hepatectomy; D022781:Hepatocytes / Q000637:transplantation*; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D008026:Ligation; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000502:physiology
chemical_list: D006003:Glycogen