Histological co-localization of iron in Abeta plaques of PS/APP transgenic mice.
This study confirms the presence of iron, co-localized with Abeta plaques, in PS/APP mouse brain, using Perls' stain for Fe3+ supplemented by 3,3'-diaminobenzidine (DAB) and Abeta immunohistochemistry in histological brains sections fixed with formalin or methacarn. In this study, the fixation process and the slice thickness did not interfere with the Perls' technique. The presence of iron in beta-amyloid plaques in PS/APP transgenic mice, a model of Alzheimer's disease (AD) pathology, may explain previous reports of reductions of transverse relaxation time (T2) in MRI studies and represent the source of the intrinsic Abeta plaque MR contrast in this model.
Falangola
Maria F
MF
Center for Advanced Brain Imaging, Nathan Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA. falangola@nki.rfmh.org
Lee
Sang-Pil
SP
Nixon
Ralph A
RA
Duff
Karen
K
Helpern
Joseph A
JA
D015100:3,3'-Diaminobenzidine; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002421:Caudate Nucleus / Q000473:pathology; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000378:metabolism* / Q000473:pathology; D011699:Putamen / Q000378:metabolism; D016707:Tissue Fixation
D016229:Amyloid beta-Peptides; D015100:3,3'-Diaminobenzidine; D007501:Iron