Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope.
Receptor-mediated gene transfer using an M cell ligand has been shown to be an efficient method for mucosal DNA immunization. To investigate further into alternative M cell ligands, the plant lectin, Ulex europaeus agglutinin I (UEA-1), was tested. UEA-1 binds to human intestinal Caco-2 cells, and these cells can be transfected with poly-l-lysine (PL)-conjugated UEA-1 for expression of reporter cDNAs. When tested in vivo, mice nasally immunized with UEA-1-PL complexed to plasmid encoding HIV-1 envelope showed elevated systemic and mucosal antibody responses, and these were supported by tissue antibody-forming cells. Likewise, elevated envelope-specific CTLs were induced. Thus, UEA-1 mediated DNA delivery represents an alternative mucosal formulation for inducing humoral and cellular immunity against HIV-1.
Wang
Xinhai
X
Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717-3610, USA.
Kochetkova
Irina
I
Haddad
Asmahan
A
Hoyt
Teri
T
Hone
David M
DM
Pascual
David W
DW
D016915:AIDS Vaccines / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D018938:Caco-2 Cells; D015483:HIV Antibodies / Q000032:analysis; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000276:immunology; D037121:Plant Lectins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D039762:Ulex; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic
D016915:AIDS Vaccines; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic