title: Molecular taxonomy of a soil actinomycete isolate, KCCM10454 showing neuroprotective activity by 16S rRNA and rpoB gene analysis.
abstract: Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)442, according to the results of 16S rRNA and rpoB gene analyses.
authors.affiliation: Department of Anatomy & Neurobiology, College of Medicine, Institute of Medical Science, Cheju National University, Jeju 690-756, Republic of Korea.
authors.forename: Bong-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Hong
authors.initials: H
authors.lastname: Kim
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yoon-Kyu
authors.initials: YK
authors.lastname: Lim
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Byun
authors.forename: Brian
authors.initials: B
authors.lastname: Hutchinson
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Ko
authors.forename: Keun-Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Cha
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000302:isolation & purification*; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D018696:Neuroprotective Agents / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000187:drug effects / Q000235:genetics
chemical_list: D000927:Anticonvulsants; D018696:Neuroprotective Agents; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin