PPARgamma-agonist rosiglitazone increases number and migratory activity of cultured endothelial progenitor cells.
OBJECTIVE
Endothelial progenitor cells (EPC) are involved in the process of endothelial maintenance and angiogenesis and might be related to endothelial function. EPC function was shown to be impaired in type 2 diabetic patients. Since endothelial dysfunction of type 2 diabetic patients can be ameliorated by treatment with thiazolidinediones we asked whether this treatment might also influence number and function of EPC.


RESULTS
We investigated 10 recently diagnosed type 2 diabetic patients and 10 age and sex matched healthy control subjects. After baseline examination of metabolic parameters and EPC, patients received 4 mg rosiglitazone b.i.d. for 12 weeks. We measured EPC number and migratory activity after 3 and 12 weeks of treatment. Migratory activity of EPCs obtained from type 2 diabetic patients at baseline was 40% lower compared to control (P<0.05). There was no significant difference of EPC number between patients (323+/-19) and controls (358+/-25) at baseline. Treatment of patients with rosiglitazone normalized impaired migratory activity of EPC and increased EPC number (464+/-33, P<0.01). In addition treatment improved glycemic control and insulin sensitivity.


CONCLUSIONS
Twelve-week treatment with rosiglitazone improved EPC number and migratory activity of type 2 diabetic patients. The latter mechanism may contribute to the recently observed improvement of endothelial function by rosiglitazone in type 2 diabetes.
Pistrosch
Frank
F
Nephrology, Department of Medicine, University Hospital Dresden, Fetscherstrasse 74, Dresden 01307, Germany. frankpistrosch@hotmail.com
Herbrig
Kay
K
Oelschlaegel
Uta
U
Richter
Susannne
S
Passauer
Jens
J
Fischer
Sabine
S
Gross
Peter
P
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005260:Female; D015309:Glucose Clamp Technique; D006442:Glycated Hemoglobin / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood; D007333:Insulin Resistance; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D047495:PPAR gamma / Q000819:agonists*; D010455:Peptides / Q000032:analysis; D000077154:Rosiglitazone; D016037:Single-Blind Method; D045162:Thiazolidinediones / Q000494:pharmacology* / Q000627:therapeutic use; D014280:Triglycerides / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D002784:Cholesterol; D006442:Glycated Hemoglobin; D006023:Glycoproteins; D008077:Lipoproteins, LDL; D047495:PPAR gamma; D010455:Peptides; D000077154:Rosiglitazone; D045162:Thiazolidinediones; D014280:Triglycerides; D042461:Vascular Endothelial Growth Factor A; C032629:acetyl-LDL