Effect of Cysticercus cellulosae fractions on the respiratory burst of pig neutrophils.
Neutrophils, eosinophils and macrophages are cells that interact with invading parasites and naive hosts have been shown to have anti-parasitic activity. The initial reaction of these leukocytes is the generation of reactive oxygen species (ROS) to play in parasite expulsion. The present work was carried out to study the effect of total extract, scolex and membrane fractions from Cysticercus cellulosae on respiratory burst by pig neutrophils. Hydrogen peroxide (H2O2) production by neutrophils incubated with metacestode fractions from C. cellulosae showed an increase of: 190% (total extract), 120% (scolex) and 44% (membrane). High antioxidant catalatic activity (33%, 28%, 28% by total extract, scolex and membrane, respectively) was observed in neutrophils incubated with metacestode fractions, which could be an attempt at self-protection. Scolex and membrane fractions increased the phagocytic capacity of neutrophils (44% and 28%, respectively). On the other hand, total cysticerci did not alter the phagocytosis, possibly due to modifications in membrane function, caused by high ROS production from neutrophils in the presence of total cysticerci. Total fraction from C. cellulosae is toxic for neutrophils as shown by the decrease in phagocytic capacity, probably caused by high levels of ROS formation. The difference in toxicity of total extract, scolex and membrane fractions on neutrophils can be explained by the presence of an antigenic effect of the vesicular fluid in the total extract of C. cellulosae.
Pugine
Silvana Marina Piccoli
SM
Department of Basic Science, Faculty of Zootechny and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Faria
Michelle Fernandes de
MF
Maia
Antônio Augusto Mendes
AA
Valle
Claudia Ribeiro do
CR
Boschini
Camila
C
Poleti
Mirele Daiana
MD
Silva
Marcia Ramos Monteiro da
MR
De Melo
Mariza Pires
MP
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000469:parasitology; D003552:Cysticercus / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000469:parasitology* / Q000502:physiology; D010088:Oxidoreductases / Q000096:biosynthesis*; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology*; D016897:Respiratory Burst; D013552:Swine
D000947:Antigens, Helminth; D017382:Reactive Oxygen Species; D010088:Oxidoreductases