title: Description of Enterobacter ludwigii sp. nov., a novel Enterobacter species of clinical relevance.
abstract: A new species, Enterobacter ludwigii, is presented on the basis of the characteristics of 16 strains, which were isolated from clinical specimens. These bacteria form a distinct genetic cluster in phylogenetic analyses of the population structure of the Enterobacter cloacae complex. As determined by DNA-DNA cross-hybridization experiments in microplates, this genetic cluster can be delineated from the other species of the E. cloacae complex with deltaTm values equal to or above 5 degrees C with Enterobacter hormaechei being the closest relative. The bacteria are gram-negative, fermentative, motile rods with the general characteristics of the genus Enterobacter and the E. cloacae complex in particular. E. ludwigii can be differentiated from the other Enterobacter species by its growth on myo-inositol and 3-0-methyl-D-glucopyranose. The type strain is EN-119 (= DSM 16688T = CIP 108491T).
authors.affiliation: Institute of Microbiology and Laboratory Medicine, Pneumatological Hospital, Robert-Koch-Allee 2, D-82131 Gauting, Germany. harald.hoffmann@asklepios.com
authors.forename: Harald
authors.initials: H
authors.lastname: Hoffmann
authors.forename: Sibylle
authors.initials: S
authors.lastname: Stindl
authors.forename: Anita
authors.initials: A
authors.lastname: Stumpf
authors.forename: Andre
authors.initials: A
authors.lastname: Mehlen
authors.forename: Daniel
authors.initials: D
authors.lastname: Monget
authors.forename: Jürgen
authors.initials: J
authors.lastname: Heesemann
authors.forename: Karl H
authors.initials: KH
authors.lastname: Schleifer
authors.forename: Andreas
authors.initials: A
authors.lastname: Roggenkamp
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004754:Enterobacter / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005285:Fermentation; D020459:Genes, rRNA; D005840:Gentian Violet; D006801:Humans; D007294:Inositol / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D007294:Inositol; D005840:Gentian Violet