title: [Significance of the DNA-HPV research in cervical cancer prevention].
abstract: The purpose of our research was to assess DNA-HPV frequention observation and evaluation of the diagnostic value mRNA E6 and E7 HPV16 and HPV18 profile concentration in prognostic risk of intraepithelial lesions and cervical cancer progression in women with cytological screening. Human papilloma virus (HPV) infection were detected in 13.8% normal samples, presence HPV16 and 18 in 7.5% samples were detected. HPV 16, 18 or HPV16 and 18 infection were detected in 85% HSIL (high-grade squamous intraepithelial lesion) samples. HPV16 or HPV18 infection in 100% cancer samples were detected. In samples from control group expression of E6 and E7 genes were not detected. In LSIL (low-grade squamous intraepithelial lesion) group HPV16 E7 gene in 2.6% samples, in HSIL group E7 gene in 9.5% samples were detected. In all cancer samples E7 or E6 HPV16 and/or HPV18 were detected.
authors.affiliation: Z Katedry i Oddziału Klinicznego Ginekologii i Połoznictwa w Tychach.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Michalski
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Joanna
authors.initials: J
authors.lastname: Orchel
authors.forename: Agata
authors.initials: A
authors.lastname: Kachel-Flis
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zieliński
authors.forename: Adrian
authors.initials: A
authors.lastname: Lukasik
authors.forename: Anna
authors.initials: A
authors.lastname: Belowska
authors.forename: Mateusz
authors.initials: M
authors.lastname: Michalski
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral; D027383:Papillomaviridae / Q000235:genetics*; D011044:Poland; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000517:prevention & control / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000517:prevention & control / Q000821:virology*
chemical_list: D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012097:Repressor Proteins; C069102:oncogene protein E2, Human papillomavirus type 18