Event-specific real-time detection and quantification of genetically modified Roundup Ready soybean.
The event-specific real-time detection and quantification of Roundup Ready soybean (RRS) using an ABI PRISM 7700 sequence detection system with light upon extension (LUX) primer was developed in this study. The event-specific primers were designed, targeting the junction of the RRS 5' integration site and the endogenous gene lectin1. Then, a standard reference plasmid was constructed that carried both of the targeted sequences for quantitative analysis. The detection limit of the LUX real-time PCR system was 0.05 ng of 100% RRS genomic DNA, which was equal to 20.5 copies. The range of quantification was from 0.1 to 100%. The sensitivity and range of quantification successfully met the requirement of the labeling rules in the European Union and Taiwan.
Huang
Chia-Chia
CC
Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan 106.
Pan
Tzu-Ming
TM
D018744:DNA, Plant / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000235:genetics*
D018744:DNA, Plant