title: Semiquantitative human papillomavirus type 16 viral load and the prospective risk of cervical precancer and cancer.
abstract: We examined whether higher human papillomavirus type 16 (HPV16) viral load predicted risk of cervical intraepithelial neoplasia 3 (CIN3) or cancer (together termed > or =CIN3) within a cohort of 20,810 women followed for 10 years with cytologic screening. Semiquantitative viral load for HPV16 was measured on baseline cervicovaginal specimens using a type-specific hybridization probe test with signal amplification. An increased risk of > or =CIN3 associated with higher HPV16 viral load was found only among cytologically negative women in early follow-up, suggesting that these cases were related to the detection of prevalent lesions missed at baseline. Women with higher HPV16 viral load were more likely to undergo ablative treatment during follow-up than those with lower viral load (P(trend) = 0.008), possibly diminishing any additional risk for > or =CIN3 attributable to higher HPV16 viral loads.
authors.affiliation: Digene Corporation, 1201 Clopper Road, Gaithersburg, MD 20878, USA.
authors.forename: Philip E
authors.initials: PE
authors.lastname: Castle
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
authors.forename: David R
authors.initials: DR
authors.lastname: Scott
authors.forename: Mark E
authors.initials: ME
authors.lastname: Sherman
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Glass
authors.forename: Brenda B
authors.initials: BB
authors.lastname: Rush
authors.forename: John E
authors.initials: JE
authors.lastname: Schussler
authors.forename: Sholom
authors.initials: S
authors.lastname: Wacholder
authors.forename: Attila T
authors.initials: AT
authors.lastname: Lorincz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D052162:Human papillomavirus 16 / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008875:Middle Aged; D009922:Oregon / Q000453:epidemiology; D011446:Prospective Studies; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D014626:Vaginal Smears; D019562:Viral Load; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral