title: Coculture with dendritic cells promotes proliferation but not cytotoxic activity of gamma/delta T cells.
abstract: T cells bearing the gamma9/delta2 T cell receptor (TCR) have recently raised interest as non-MHC restricted effector cells against multiple myeloma. They are described to be stimulated by phosphoantigens without the need of antigen presenting cells. However, in the past a positive effect of cells of the monocyte lineage on activation of gamma/delta T cells has been shown. Monocyte derived dendritic cells (DC) are professional antigen presenting cells widely investigated as stimulators of alpha/beta T cells. But only little is known about the interaction of gamma/delta T cells and monocyte derived DC. Here, we investigated the effect of coculture of mature DC unpulsed or pulsed with ibandronate on the proliferation and cytotoxic activity of isolated gamma/delta T cells. After coculturing monocyte derived DC with isolated gamma/delta T cells, proliferation of gamma/delta T cells was enhanced as determined by the (3)H thymidine uptake assay. Also, IFN-gamma secretion was increased after coculture with DC. As DC are well known to induce activation of alpha/beta T cells we investigated whether the cytotoxic activity of gamma/delta T cells could be increased by coculture with DC. We found no difference in cytotoxic activity of gamma/delta T cells alone or cocultured with unpulsed or pulsed mature DC. Also, sensitizing of myeloma cells by addition of ibandronate could not increase lysis by gamma/delta T cells. In conclusion, monocyte derived DC are capable of stimulating proliferation and secretion of IFN-gamma of gamma/delta T cells but do not exert an effect on cytotoxic activity of gamma/delta T cells against myeloma cells.
authors.affiliation: Department of Medicine, Bonn University, Germany. m.lilienfeld.toal@uni-bonn.de
authors.forename: M
authors.initials: M
authors.lastname: von Lilienfeld-Toal
authors.forename: E
authors.initials: E
authors.lastname: Sievers
authors.forename: V
authors.initials: V
authors.lastname: Bodemüller
authors.forename: C
authors.initials: C
authors.lastname: Mihailescu
authors.forename: A
authors.initials: A
authors.lastname: Märten
authors.forename: M
authors.initials: M
authors.lastname: Gorschlüter
authors.forename: I G H
authors.initials: IG
authors.lastname: Schmidt-Wolf
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004164:Diphosphonates / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000187:drug effects; D009000:Monocytes / Q000166:cytology; D009101:Multiple Myeloma / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D004164:Diphosphonates; D016692:Receptors, Antigen, T-Cell, gamma-delta