Expression of the Rho-family GTPase gene RHOF in lymphocyte subsets and malignant lymphomas.
We have studied the expression of RHOF, a member of the Rho-GTPase family, in an array of lymphoid cells and tissues. Previous microarray studies demonstrated RHOF upregulation in a subset of transformed follicular lymphomas. Real-time quantitative polymerase chain reaction evaluated RHOF expression in lymphocyte subpopulations, and normal and malignant lymphoid tissue. Cells and tissues of B-cell origin expressed higher RHOF levels than their T-cell counterparts. Neoplastic cells and tissues of B-cell origin expressed higher levels of RHOF than their benign cellular counterparts. Relatively elevated levels of RHOF were seen in lymphomas derived from germinal centre origin.
Gouw
Launce G
LG
Department of Hematology, University of Utah, 50 North Medical Drive, Salt Lake City, UT 84132, USA.
Reading
N Scott
NS
Jenson
Stephen D
SD
Lim
Megan S
MS
Elenitoba-Johnson
Kojo S J
KS
D000704:Analysis of Variance; D001402:B-Lymphocytes / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015870:Gene Expression; D018858:Germinal Center / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics; D015458:Leukemia, T-Cell / Q000235:genetics; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000378:metabolism; D008223:Lymphoma / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics; D020522:Lymphoma, Mantle-Cell / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics* / Q000276:immunology
C419867:RHOF protein, human; D020741:rho GTP-Binding Proteins