Reduced expression of chemokine (C-C motif) ligand-2 (CCL2) in ovarian adenocarcinoma.
Chemokine (C-C motif) ligand-2 (CCL2) is a chemoattractant and activator of macrophages and is a key determinant of the macrophage infiltrate into tumours. We demonstrate here that CCL2 is expressed in normal human ovarian surface epithelium (HOSE) cells and is silenced in most ovarian cancer cell lines, and silenced or downregulated in the majority of primary ovarian adenocarcinomas. Analysis of the CCL2 locus at 17q11.2-q12 showed loss of heterozygosity (LOH) in 70% of primary tumours, and this was significantly more common in tumours of advanced stage or grade. However, we did not detect any mutations in the CCL2 coding sequence in 94 primary ovarian adenocarcinomas. These data support the hypothesis that CCL2 may play a role in the pathobiology of ovarian cancers, but additional studies will be required to evaluate this possibility.
Arnold
J M
JM
The Queensland Institute of Medical Research, c/o RBH Post Office, Herston, Brisbane, QLD 4029, Australia. jeremyA@qimr.edu.au
Huggard
P R
PR
Cummings
M
M
Ramm
G A
GA
Chenevix-Trench
G
G
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D020868:Gene Silencing; D006801:Humans; D019656:Loss of Heterozygosity; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
C111835:CCL2 protein, human; D018932:Chemokine CCL2