Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiae.
Post-translational histone modifications abound and regulate multiple nuclear processes. Most modifications are targeted to the amino-terminal domains of histones. Here we report the identification and characterization of acetylation of lysine 56 within the core domain of histone H3. In the crystal structure of the nucleosome, lysine 56 contacts DNA. Phenotypic analysis suggests that lysine 56 is critical for histone function and that it modulates formamide resistance, ultraviolet radiation sensitivity, and sensitivity to hydroxyurea. We show that the acetylated form of histone H3 lysine 56 (H3-K56) is present during interphase, metaphase, and S phase. Finally, reverse genetic analysis indicates that none of the known histone acetyltransferases is solely responsible for H3-K56 acetylation in Saccharomyces cerevisiae.
Ozdemir
Anil
A
Department of Molecular Biology, Nijmegen Center for Molecular Life Sciences, Radboud University, 6500 HB Nijmegen, The Netherlands.
Spicuglia
Salvatore
S
Lasonder
Edwin
E
Vermeulen
Michiel
M
Campsteijn
Coen
C
Stunnenberg
Hendrik G
HG
Logie
Colin
C
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005559:Formamides / Q000494:pharmacology; D006657:Histones / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006859:Hydrogen; D007399:Interphase; D008239:Lysine / Q000737:chemistry*; D008677:Metaphase; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D017354:Point Mutation; D011485:Protein Binding; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D005559:Formamides; D006657:Histones; C031066:formamide; D006859:Hydrogen; D004247:DNA; D008239:Lysine