Virulence and molecular diversity in Colletotrichum graminicola from Brazil.
Genetic diversity among 37 isolates of the sorghum anthracnose pathogen Colletotrichum graminicola, from four geographically distinct regions of Brazil, was evaluated by RAPD and RFLP-PCR markers and virulence characters on a set of 10 differential sorghum genotypes. Twenty-two races were identified and race 13B was the most frequent, but present in only two regions. RAPD analysis revealed 143 polymorphic bands that grouped the isolates according to their geographic origin, but not by their virulence phenotypes. RFLP with HaeIII, MspI, HinfI, HhaI, HpaII, EcoRI, HindIII, PstI, RsaI, Taq alphaI, and AluI enzymes over ITS domains and 5.8 rDNA genes of C. graminicola did not show differences among the isolates, indicating high conservation of these restriction sites. Molecular polymorphism was observed among isolates belonging to the same race. No association between virulence phenotypes and molecular profiles was observed.
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Departmento de Microbiologia, Instituto de Ciências Biològicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil. hmvaler@icb.ufmg.br
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Weikert-Oliveira
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Casela
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D001938:Brazil; D020231:Colletotrichum / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005838:Genotype; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D045868:Sorghum / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer