Ligand-dependent protein interactions of the estrogen receptors using the yeast two-hybrid system.
The protein-interacting ability of estrogen receptor beta (ERbeta) and estrogen receptor alpha (ERalpha) from Atlantic salmon (Salmo salar) was studied using the yeast two-hybrid system. The salmon ERbeta shared features with other nuclear receptors regarding dimerization, forming a homodimer and heterodimer with ERalpha. The activation of salmon ERs by estradiol-17beta was shown to be necessary for dimerization. Furthermore, the xenoestrogens, 4-nonylphenol and bisphenol A, were shown to promote ER dimerization in the yeast two-hybrid system.
Oftedal
Bergithe E
BE
Department of Molecular Biology, University of Bergen, Norway.
Ladstein
Sigve
S
Telle
Wenche
W
Male
Rune
R
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D008024:Ligands; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D020317:Salmo salar; D020798:Two-Hybrid System Techniques
D008024:Ligands; D011960:Receptors, Estrogen