title: [Effects of Shengji Huayu Recipe and its decomposed formulas on synthesis of collagen types I and III in granulation tissue of rats in early wound healing].
abstract: OBJECTIVE
  To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types I and III in granulation tissue of rats in early wound healing.
  
  
  METHODS
  Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types I and III in granulation tissue of the rats were tested with immunohistochemical methods and image analysis.
  
  
  RESULTS
  On the third day of wound healing, collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen I of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen III of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen III of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05).
  
  
  CONCLUSIONS
  Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.
authors.affiliation: Department of Chinese Traditional Surgery, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. drlibin@hotmail.com
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Zhen-Yi
authors.initials: ZY
authors.lastname: Wang
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Xiao
authors.forename: Fu-Lun
authors.initials: FL
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Fan
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000503:physiopathology; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006097:Granulation Tissue / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal