The protein ICP0 of herpes simplex virus type 1 is targeted to nucleoli of infected cells. Brief report.
This study describes the nucleolar localization of the viral protein ICP0 of herpes simplex virus type 1. We show that the RING finger domain of ICP0 is essential for ICP0 to localize in nucleoli of transfected and 4 hour-infected cells. ICP0 forms particular intranucleolar domains that do not correspond to any known nucleolar domains. This distribution was confirmed by immunoblots performed on fractionated infected cells. Quantitative RT-PCR experiments indicated that ICP0 did not increase the transcription from the RNA polymerase I (Pol I) promoter in transfected cells, an effect opposite to that observed on viral and cellular Pol II promoters. Nucleoli are thus, after PML bodies and centromeres, a novel nuclear structure targeted by ICP0.
Morency
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Centre de Génétique Moléculaire et Cellulaire, UMR5534-CNRS, Equipe Silencing Viral et Remodelage de la Chromatine Université Claude Bernard Lyon 1, Villeurbanne, France.
Couté
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Thomas
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J
Texier
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Lomonte
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P
D002466:Cell Nucleolus / Q000821:virology*; D002503:Centromere / Q000821:virology; D006367:HeLa Cells; D006561:Herpes Simplex / Q000503:physiopathology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000821:virology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D016335:Zinc Fingers
D014764:Viral Proteins; C095533:gene 63 protein, Equine herpesvirus 1