P5644 interacts with phosphatidylinositol-4-phosphate adaptor protein-1 associated protein-1.
The human novel gene pp5644 (GeneBank Accession No. AF289559) coding for 124 amino acids was recently cloned. Overexpression of pp5644 in Hela cells significantly inhibited the growth and colony formation. The pp5644-interacting protein FAPP1 (phosphatidylinositol-four-phosphate adaptor protein1) associated protein-1, called FASP1, was obtained by using yeast two-hybrid system. The interaction between pp5644 and FASP1 was experimentally confirmed by GST pull-down assay in vitro and co-immunoprecipitation assay in vivo. Co-localization of pp5644 and FASP1 in cytoplasm in Hela cells could further support the interaction. Based on the experimental results, it is suggested that pp5644 physically bind to FASP1 and the biological significance of this kind of interaction in vivo is discussed.
Ye
Xiao-Xia
XX
State Key Laboratory of Genetic Engineering, Institute of Genetic, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
Lu
Hong
H
Yu
Yao
Y
Ding
Ning
N
Zhang
Nai-Ling
NL
Huo
Ke-Ke
KK
Wan
Da-Fang
DF
Li
Yu-Yang
YY
Gu
Jian-Ren
JR
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C502943:MAFIP protein, human; C502942:MIS18A protein, human; C487037:PLEKHA3 protein, human; D011506:Proteins; D025521:Tumor Suppressor Proteins; D005982:Glutathione Transferase