Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43.
Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a > or =4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.
Che
Xiao-Yan
XY
Center of Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, People's Republic of China. linche@pub.guangzhou.gd.cn
Qiu
Li-Wen
LW
Liao
Zhi-Yong
ZY
Wang
Ya-di
YD
Wen
Kun
K
Pan
Yu-Xian
YX
Hao
Wei
W
Mei
Ya-Bo
YB
Cheng
Vincent C C
VC
Yuen
Kwok-Yung
KY
D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood*; D028941:Coronavirus 229E, Human / Q000276:immunology*; D028962:Coronavirus OC43, Human / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M