title: Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies.
abstract: We have demonstrated the feasibility of detecting and quantifying six cell-cycle-related nuclear markers (Ki67, pRb, p27, phospho-p27 (phosphorylated p27), phospho-pRb (phosphorylated pRb), phospho-HH3 (phosphorylated histone H3)) in plucked human scalp and eyebrow hair. Estimates of the proportion of plucked hairs that are lost or damaged during processing plus the intra- and intersubject variability of each nuclear marker with these techniques are provided to inform sizing decisions for intervention studies with drugs potentially impacting on these markers in the future.
authors.affiliation: Edinburgh Cancer Centre, Western General Hospital, Edinburgh EH4 2XU, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Camidge
authors.forename: K R
authors.initials: KR
authors.lastname: Randall
authors.forename: J R
authors.initials: JR
authors.lastname: Foster
authors.forename: C J
authors.initials: CJ
authors.lastname: Sadler
authors.forename: J A
authors.initials: JA
authors.lastname: Wright
authors.forename: A R
authors.initials: AR
authors.lastname: Soames
authors.forename: P J
authors.initials: PJ
authors.lastname: Laud
authors.forename: P D
authors.initials: PD
authors.lastname: Smith
authors.forename: A M
authors.initials: AM
authors.lastname: Hughes
mesh_terms: D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation; D023381:Endpoint Determination; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013048:Specimen Handling
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers