title: Relationship between Porphyromonas gingivalis, Epstein-Barr virus infection and reactivation in periodontitis.
abstract: The aim of this study was to assess the relationship-between Porphyromonas gingivalis, Epstein-Barr virus (EBV) infection and reactivation in periodontitis using real-time PCR. The mean proportion of P. gingivalis cells to total bacterial cells in the saliva from EBV-positive periodontitis patients was significantly higher than that in saliva from EBV-negative patients. An EBV-positive B-cell line was used to determine whether P. gingivalis sonicate induced reactivation of EBV, using real-time PCR to measure the virus genome in the culture medium. A significant increase in EBV numbers was observed after the stimulation with P. gingivalis sonicate. These findings suggest that the interaction between EBV and P. gingivalis is bi-directional, with EBV reactivation suppressing host defenses and permitting overgrowth of P. gingivalis, and P. gingivalis having the potential to induce EBV reactivation.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan. sugano-n@dent.nihon-u.ac.jp
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sugano
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ikeda
authors.forename: Maiko
authors.initials: M
authors.lastname: Oshikawa
authors.forename: Masataka
authors.initials: M
authors.lastname: Idesawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Tanaka
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000821:virology; D002460:Cell Line / Q000821:virology; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D010518:Periodontitis / Q000209:etiology / Q000382:microbiology* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000472:pathogenicity* / Q000502:physiology; D012463:Saliva / Q000382:microbiology / Q000821:virology; D018709:Statistics, Nonparametric; D014775:Virus Activation
chemical_list: D017077:Culture Media, Conditioned; D004269:DNA, Bacterial; D004279:DNA, Viral