Isolation of genes differentially expressed during the fruit body development of Pleurotus ostreatus by differential display of RAPD.
To analyze genes involved in fruit body development of Pleurotus ostreatus, mRNAs from three different developmental stages: i.e., vegetative mycelium, primordium, and mature fruit body, were isolated and reverse-transcribed to cDNAs. One hundred and twenty random PCR amplifications were performed with the cDNAs, which generated 382, 394, 393 cDNA fragments from each developmental stage. From these fragments, four cDNA clones specifically expressed in primordium or mature fruit body were detected. Sequence analysis and database searches revealed significant similarity with triacylglycerol lipase, cytochrome P450 sterol 14 alpha-demethylase and developmentally regulated genes of other fungi. Northern blot analyses confirmed that all of the four cDNAs were unexpressed in mycelium, thus stage-specific genes for fruit body formation of P. ostreatus were successfully isolated.
Sunagawa
Masahide
M
Department of Applied Microbiology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan. masahide@ffpri.affrc.go.jp
Magae
Yumi
Y
D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins