Chitosan/beta-lactoglobulin core-shell nanoparticles as nutraceutical carriers.
Chitosan (CS)/beta-lactoglobulin (betalg) core-shell nanoparticles (CS-betalg nanoparticle) were successfully prepared with the aim of developing a biocompatible carrier for the oral administration of nutraceuticals. The effects of pH and initial concentrations (C(beta)(lg)) of native and denatured betalg on the properties of the nanoparticles were investigated. Uniform nanoparticles were prepared by ionic gelation with sodium tripolyphosphate (TPP). The surface charge of the particles was positive, with a zeta potential of 20-60 mV. (beta)lg loading efficiency (LE) spanned a broad range (1-60%); and was highly sensitive to formulation pH. This adsorption can be mainly attributed to electrostatic, hydrophobic interactions and hydrogen bonding between (beta)lg and CS. Brilliant blue (BB) release experiments showed that the nanoparticles prepared with native (beta)lg had favorable properties to resist acid and pepsin degradation in simulated gastric conditions unlike those prepared with denatured (beta)lg or denatured (beta)lg crosslinked with Ca2+. When transferred to simulated intestinal conditions, the (beta)lg shells of the nanoparticles were degraded by pancreatin.
Chen
Lingyun
L
Chaire de recherche du Canada sur les protéines, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Pavillon Paul Comtois, Sainte-Foy, Qué., Canada.
Subirade
Muriel
M
D000327:Adsorption; D001557:Benzenesulfonates / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007782:Lactoglobulins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010194:Pancreatin / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D011122:Polyphosphates / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D013997:Time Factors
D001557:Benzenesulfonates; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D007477:Ions; D007782:Lactoglobulins; D011122:Polyphosphates; D010194:Pancreatin; D048271:Chitosan; D010434:Pepsin A; C006796:brilliant blue; C005692:triphosphoric acid