Renin-angiotensin system polymorphisms and coronary artery surgery patients.
The frequencies of angiotensin-converting enzyme gene insertion/deletion, angiotensinogen-M253T, and angiotensin II type 1 receptor-A1166C polymorphisms were analyzed in 105 patients undergoing coronary artery bypass grafting (group 1) and a control group of 105 non-cardiac patients (group 2). Blood samples were obtained for biochemical analyses and DNA extraction. Genotyping was performed by polymerase-chain-reaction-based restriction analysis. According to the angiotensin-converting enzyme gene insertion/deletion polymorphism, 36.3% of patients in group 1 and 30.7% in group 2 were homozygous for the DD allele. This difference was not statistically significant. Angiotensin II type 1 receptor-A1166C genotype polymorphism was also not significantly different between the groups. The results showed the angiotensinogen-M235T polymorphism to be heterogenous. The MM homozygote frequency was significantly higher in controls (72.3%), whereas 80% of the TT homozygote frequency was in the surgical group ( p = 0.001). These results show that although there were no significant differences in angiotensin-converting enzyme gene insertion/deletion and angiotensin II type 1 receptor-A1166C genotype polymorphisms between the groups, angiotensinogen-M235T polymorphism of TT homozygote frequency was significantly associated with patients undergoing coronary artery bypass surgery.
Ozisik
Kanat
K
Department of Cardiovascular Surgery, Ankara Namune Education and Research Hospital, Metis Biotechnology Ltd., Ankara, Turkey. sozisik2002@yahoo.com
Misirlioglu
Muge
M
Ulus
Tulga A
TA
Tuncer
Serdar
S
Emir
Mustafa
M
Katircioglu
Fehmi
F
D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000235:genetics* / Q000601:surgery; D004251:DNA Transposable Elements; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000235:genetics*
D004251:DNA Transposable Elements; D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen