Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry.
The diagnosis of B-cell chronic lymphoproliferative disorders is a great challenge when made in a background of polyclonal B cells. We studied the diagnostic usefulness of aberrant CD22 expression for differentiating neoplastic from benign B cells by 4-color flow cytometry. Of 56 cases of B-cell chronic lymphoproliferative disorders, we found that neoplastic cells showed aberrant CD22 expression in 39 (70%) of 56 cases, including chronic lymphocytic leukemia, mantle cell lymphoma, marginal zone lymphoma, hairy cell leukemia, and follicular lymphoma. In 4 cases, monoclonality was detected definitively only by evaluating the immunoglobulin light chain restriction in B cells with aberrant CD22 expression because numerous polyclonal B cells were present. Aberrant CD22 expression is a useful marker for detection of monoclonal B cells admixed with numerous benign polyclonal B cells.
Huang
James
J
Department of Pathology and Laboratory Medicine, Oregon Health & Science University, 97201, Portland.
Fan
Guang
G
Zhong
Yanping
Y
Gatter
Ken
K
Braziel
Rita
R
Gross
Gary
G
Bakke
Antony
A
D015703:Antigens, CD / Q000096:biosynthesis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002454:Cell Differentiation; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000276:immunology; D016130:Immunophenotyping; D037102:Lectins / Q000096:biosynthesis*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000276:immunology; D012189:Retrospective Studies; D051918:Sialic Acid Binding Ig-like Lectin 2
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D014408:Biomarkers, Tumor; C497867:CD22 protein, human; D015815:Cell Adhesion Molecules; D007147:Immunoglobulin Light Chains; D037102:Lectins; D051918:Sialic Acid Binding Ig-like Lectin 2