Induction of protein secretory pathway is required for systemic acquired resistance.
In plants, systemic acquired resistance (SAR) is established as a result of NPR1-regulated expression of pathogenesis-related (PR) genes. Using gene expression profiling in Arabidopsis, we found that in addition to controlling the expression of PR genes, NPR1 also directly controls the expression of the protein secretory pathway genes. Up-regulation of these genes is essential for SAR, because mutations in some of them diminished the secretion of PR proteins (for example, PR1), resulting in reduced resistance. We provide evidence that NPR1 coordinately regulates these secretion-related genes through a previously undescribed cis-element. Activation of this cis-element is controlled by a transcription factor that is translocated into the nucleus upon SAR induction.
Wang
Dong
D
Developmental, Cell and Molecular Biology Group, Department of Biology, Post Office Box 91000, Duke University, Durham, NC 27708, USA.
Weaver
Natalie D
ND
Kesarwani
Meenu
M
Dong
Xinnian
X
D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D004721:Endoplasmic Reticulum / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D012045:Regulatory Sequences, Nucleic Acid; D020156:Salicylic Acid / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C105496:NPR1 protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors; C077221:BIP protein, Arabidopsis; D003907:Dexamethasone; D020156:Salicylic Acid