Utility of histopathologic analysis in the evaluation of onychomycosis.
Onychomycosis is a common problem seen in clinical practice. Given the differential diagnosis of dystrophic nails, it is helpful to obtain a definitive diagnosis of dermatophyte infection before initiation of antifungal therapy. Potassium hydroxide preparation and fungal culture, which are typically used in the diagnosis of these infections, often yield false-negative results. Recent studies have suggested that nail plate biopsy with periodic acid-Schiff stain may be a very sensitive technique for the diagnosis of onychomycosis. In this article, we review the literature on the utility of histopathologic analysis in the evaluation of onychomycosis. Many of these studies indicate that biopsy with periodic acid-Schiff is the most sensitive method for diagnosing onychomycosis. We propose that histopathologic examination is indicated if the results of other methods are negative and clinical suspicion is high; therefore, it is a useful complementary technique in the diagnosis of onychomycosis.
Weinberg
Jeffrey M
JM
Clinical Research Center, Department of Dermatology, St Luke's-Roosevelt Hospital Center and Beth Israel Medical Center, New York, NY 10025, USA.
Koestenblatt
Evelyn K
EK
Jennings
Maureen B
MB
D001706:Biopsy; D005533:Foot Dermatoses / Q000175:diagnosis* / Q000191:economics / Q000473:pathology; D005658:Fungi / Q000302:isolation & purification; D006801:Humans; D006878:Hydroxides; D009262:Nails / Q000382:microbiology / Q000473:pathology*; D014009:Onychomycosis / Q000175:diagnosis* / Q000191:economics / Q000473:pathology; D010503:Periodic Acid-Schiff Reaction / Q000379:methods; D017680:Potassium Compounds; D012680:Sensitivity and Specificity
D006878:Hydroxides; D017680:Potassium Compounds; C029943:potassium hydroxide