Lipid analysis of the sex pheromone gland of the moth Heliothis virescens.
The sex pheromone gland of female Heliothis virescens was analyzed for fatty acid and lipid content. Base methanolysis of the gland showed a large amount of methyl (Z)-11-hexadecenoate (Z11-16:Acyl), the fatty acyl analog of the major pheromone component, (Z)-11-hexadecenal, as well as a small amount of methyl (Z)-11-octadecenoate. Methyl esters of various common fatty acids were also observed. HPTLC analysis of the glandular lipids revealed large quantities of triacylglycerols (TGs), and lesser amounts of 1,2-diacylglycerols (1,2-DGs), 2-monoacylglycerols (2-MGs), phosphatidyl ethanolamines, and phosphatidyl cholines. The greatest amount of Z11-16:Acyl in these lipids was in the TGs, with lesser amounts in the two phospholipid classes and only trace amounts in the other neutral lipids. The glands of females at various ages and photoperiodic times were extracted, fractionated into neutral and polar fractions by silica SPE, and fatty acid titers in these fractions determined. All fatty acids, but notably Z11-16:Acyl, showed significant total and neutral lipid fraction peaks at mid scotophase for 2-day-old females; a less dramatic, but significant, Z11-16:Acyl peak in the polar fraction was also observed. However, only a relatively small proportion (<50%) of this acid was recovered from the silica at all times. This "non-recoverable" Z11-16:Acyl showed a dramatic and significant peak at mid scotophase for 2-day females, corresponding roughly with maximal pheromone titer. All other acids in the gland were recovered in high proportions, and their respective "non-recoverable" titers were not different at any of the times analyzed. Based on previous work, this non-recoverable Z11-16:Acyl is likely the CoA ester. Therefore, it appears that the pheromone gland of H. virescens maintains pools of Z11-16:Acyl in both CoA ester and TG forms, which are available for biosynthesis of pheromone. These pools are greatest during maximal pheromone production when the biosynthetic enzymes, possibly the fatty acid reductase, are unable to utilize rapidly enough the quantities of Z11-16:Acyl biosynthesized.
Foster
S P
SP
Department of Entomology, North Dakota State University, Fargo, North Dakota 58105-5346, USA. Stephen.Foster@ndsu.nodak.edu
D000818:Animals; D002855:Chromatography, Thin Layer; D004952:Esters / Q000032:analysis; D005088:Exocrine Glands / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D009036:Moths / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012724:Sex Attractants / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D004952:Esters; D005227:Fatty Acids; D010743:Phospholipids; D012724:Sex Attractants; D014280:Triglycerides