title: Upregulation of the somatotropic axis is correlated with increased G6PDH expression in Black Sea bream adapted to iso-osmotic salinity.
abstract: Black sea bream (Mylio macrocephalus) were adapted to salinities of 0 ppt (freshwater), 6 ppt (hypo-osmotic), 12 ppt (iso-osmotic), 33 ppt (seawater), and 50 ppt (hypersaline) for 1 month. Using RT-PCR assays, the expression of pituitary growth hormone (GH) and hepatic insulin-like growth factor 1 (IGF-1) genes were studied. It was found that the transcripts for both of these genes were highest in fish maintained at iso-osmotic salinity. To correlate the expression of GH and IGF-1 with an index of growth, we also measured the transcript levels of glucose-6-phosphate dehydrogenase (G6PDH) in liver. It was found that this transcript was also elevated in iso-osmotically adapted black sea bream.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
authors.forename: Eddie E
authors.initials: EE
authors.lastname: Deane
authors.forename: Norman Y S
authors.initials: NY
authors.lastname: Woo
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis*; D013006:Growth Hormone / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D007552:Isotonic Solutions; D012462:Saline Solution, Hypertonic; D021541:Sea Bream / Q000378:metabolism*; D012623:Seawater; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; D012462:Saline Solution, Hypertonic; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D005954:Glucosephosphate Dehydrogenase