title: Identifying and characterising the Plasmodium falciparum merozoite surface protein 10 Plasmodium vivax homologue.
abstract: Plasmodium vivax malaria is one of the most prevalent parasitic diseases in Asia and Latin-America. The difficulty of maintaining this parasite culture in vitro has hampered identifying and characterising proteins implied in merozoite invasion of red blood cells. We have been able to identify an open reading frame in P. vivax encoding the Plasmodium falciparum merozoite surface protein 10 homologous protein using the partial sequences from this parasite's genome reported during 2004. This new protein contains 479 amino-acids, two epidermal growth factor-like domains, hydrophobic regions at the N- and C-termini, being compatible with a signal peptide and a glycosylphosphatidylinositol anchor site, respectively. The protein is expressed during the parasite's asexual stage and is recognised by polyclonal sera in parasite lysate using Western blot. P. vivax-infected patients' sera highly recognised recombinant protein by ELISA.
authors.affiliation: Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia.
authors.forename: Oscar
authors.initials: O
authors.lastname: Perez-Leal
authors.forename: Adriana Y
authors.initials: AY
authors.lastname: Sierra
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Barrero
authors.forename: Camilo
authors.initials: C
authors.lastname: Moncada
authors.forename: Pilar
authors.initials: P
authors.lastname: Martinez
authors.forename: Jimena
authors.initials: J
authors.lastname: Cortes
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Lopez
authors.forename: Luz M
authors.initials: LM
authors.lastname: Salazar
authors.forename: Johan
authors.initials: J
authors.lastname: Hoebeke
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000737:chemistry*; D010966:Plasmodium vivax / Q000737:chemistry*; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D015800:Protozoan Proteins