Fra-1 activity in the frog, Rana esculenta, testis.
Using an anti-Fos family member antiserum, we previously described, in the testis of Rana esculenta, the presence of a nuclear 43-kDa protein that we hypothesized to be Fra-1. Using an antiserum against Fra-1, we here report on Fra-1 expression, localization, and putative activity in the R. esculenta testis during the annual reproductive cycle. Western blot analysis confirms that the nuclear 43-kDa protein is Fra-1. Immunocytochemistry demonstrates Fra-1 in peritubular myoid cells (PMC), efferent ducts, and blood vessels. We present, for the first time for a vertebrate, experimental evidence that the expression of Fra-1 in PMC is related to its activity during sperm transport from the tubular compartment to the efferent ducts.
Cobellis
Gilda
G
Dipartimento di Medicina Sperimentale, Sez. F. Bottazzi, II Università di Napoli, Naples, Italy.
Lombardi
Monica
M
Scarpa
Donatella
D
Izzo
Gaia
G
Fienga
Giulia
G
Meccariello
Rosaria
R
Pierantoni
Riccardo
R
Fasano
Silvia
S
D000818:Animals; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis*; D011893:Rana esculenta / Q000378:metabolism*; D013737:Testis / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D000099169:Fos-Related Antigen 1
D016760:Proto-Oncogene Proteins c-fos; D000099169:Fos-Related Antigen 1