Alginate lyase (AlgL) activity is required for alginate biosynthesis in Pseudomonas aeruginosa.
To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algLdelta::Gm(r) mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.
Albrecht
Mark T
MT
Division of Biomedical Sciences, University of California, Riverside, Riverside, California 92521-0121, USA.
Schiller
Neal L
NL
D000464:Alginates / Q000378:metabolism; D005816:Genetic Complementation Test; D020723:Glucuronic Acid / Q000096:biosynthesis* / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D009154:Mutation; D011108:Polymers / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D014574:Uronic Acids / Q000378:metabolism
D000464:Alginates; D006603:Hexuronic Acids; D011108:Polymers; D014574:Uronic Acids; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase