[Clinical observation and expression of C-reactive protein by LPS-induced A549 cells in patients with chronic obstructive pulmonary disease].
OBJECTIVE
To investigate the level of mRNA expression of C-reactive proteins (CRP) induced by LPS in human alveolar type epithelial cell line, A549.


METHODS
Sputum and plasma specimens were obtained from patients with chronic obstructive pulmonary disease (COPD) diagnosed according to the national criteria. CRP was measured by enzyme-linked immunosorbent assays (ELISA). A549 cells were incubated with lipopolysaccharide (LPS) at different concentrations and for different time. CRP mRNA was extracted from cells and the expression was analyzed by RT-PCR. The CRP in the supernatant was measured by ELISA.


RESULTS
The concentration of CRP in the sputum of patients with COPD was significantly higher than that in the plasma (P < 0.05). LPS at different concentrations and for different time induced the expression and secretion of CRP in A549 with a time-dependent increase and the expression of CRP were significantly higher than those of the control (P < 0.05 - 0.01).


CONCLUSIONS
Self-secretion of CRP in the respiratory tract may exist. A549 induced by LPS may express CRP mRNA.
Wu
Shang-jie
SJ
Department of Respiratory disease, Second Xiangya Hospital, Central South University, Changsha 410011, China. wushangjie@sina.com
Zhao
Shui-ping
SP
D002097:C-Reactive Protein / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013183:Sputum / Q000378:metabolism
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D002097:C-Reactive Protein