Localization and quantification of fluorescent inclusions embedded in a turbid medium.
A set-up, based on a CCD camera, to localize fluorescent inclusions in diffusing media was developed. This set-up allows one to acquire a huge dataset along two axes. This aspect is fundamental to performing a tomographic reconstruction in order to quantify the fluorescence amplitude in each voxel of the sample. Firstly, a simple analytical approach to recover the position of a single inclusion, embedded in a turbid medium, was developed. Then, we implemented a reconstruction algorithm to recover the position of one and two inclusions and to estimate their relative concentrations. Finally, we studied the dependence of reconstructed data on the number of injection points of excitation light and the number of detection points of fluorescence emission.
D'Andrea
Cosimo
C
INFM-Dipartimento di Fisica and IFN-CNR, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milan, Italy. cosimo.dandrea@polimi.it
Spinelli
Lorenzo
L
Comelli
Daniela
D
Valentini
Gianluca
G
Cubeddu
Rinaldo
R
D000465:Algorithms; D000818:Animals; D005456:Fluorescent Dyes; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D002479:Inclusion Bodies / Q000378:metabolism* / Q000648:ultrastructure*; D008163:Luminescent Measurements / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes