Affinity ligands for industrial protein purification.
Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
Linhult
Martin
M
Department of Biotechnology, Royal Institute of Technology (KTH), Albanova University Center, Stockholm, Sweden. martinl@biotech.kth.se
Gülich
Susanne
S
Hober
Sophia
S
D002617:Chemical Industry / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D008024:Ligands; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000302:isolation & purification*
D008024:Ligands; D011506:Proteins