title: A chitosan-arginine conjugate as a novel anticoagulation biomaterial.
abstract: Chitosan (CS) was modified with arginine using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as coupling agents. FTIR and 13C NMR spectra showed that arginine was chemically coupled to CS to form a chitosan-arginine conjugate (CS-ArgC). The substitution degree of arginine in CS estimated from elemental analysis was 20.1%. The circular dichroism spectra indicated that the incorporation of arginine significantly altered the conformation of thrombin; while no obvious variation in the conformation of thrombin was observed with the addition of CS. The anticoagulation activity of glucose aldehyde crosslinked CS-ArgC and CS membranes was evaluated by assaying prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT). The APTT of CS-ArgC membrane was prolonged two times as that of CS counterpart, suggesting that the CS-ArgC is a promising candidate as an anticoagulation biomaterial.
authors.affiliation: Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China. wgliu@tju.edu.cn
authors.forename: W G
authors.initials: WG
authors.lastname: Liu
authors.forename: J R
authors.initials: JR
authors.lastname: Zhang
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Cao
authors.forename: F Y
authors.initials: FY
authors.lastname: Xu
authors.forename: K D
authors.initials: KD
authors.lastname: Yao
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D001120:Arginine / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D005240:Feasibility Studies; D006801:Humans; D008422:Materials Testing
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D048271:Chitosan; D001120:Arginine