Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde.
This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols.
Lim
Eng-Kiat
EK
CNAP, Department of Biology, University of York, UK.
Jackson
Rosamond G
RG
Bowles
Dianna J
DJ
D000171:Acrolein / Q000031:analogs & derivatives; D000447:Aldehydes / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
D000447:Aldehydes; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C501624:sinapyl aldehyde; C075384:coniferaldehyde; D000171:Acrolein; D016695:Glycosyltransferases; D005964:Glucosyltransferases; C000592771:UGT72E1 protein, Arabidopsis