Solid-phase synthesis of oligonucleotides labeled with luminescent lanthanide(III) chelates.
The synthesis of phosphoramidite building blocks that allow introduction of luminescent europium(III), terbium(III), dysprosium(III), and samarium(III) chelates to oligonucleotides on the solid phase is described. Several labeled oligonucleotides using these building blocks were prepared, and the photophysical properties of these bioconjugates were investigated.
Jaakkola
Lassi
L
PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Peuralahti
Jari
J
Hakala
Harri
H
Kunttu
Jenni
J
Tallqvist
Pekka
P
Mukkala
Veli-Matti
VM
Ylikoski
Alice
A
Hovinen
Jari
J
D002614:Chelating Agents / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D009841:Oligonucleotides; D011725:Pyridines; C023666:pyridine