[Study of DNA-binding proteins in mitochondria of rat liver gamma-irradiation].
Acid-soluble proteins were isolated from the liver mitochondria of control and irradiated (8 Gy) rats. By means of electrophoresis in 15% polyacrylamide gel, these proteins were separated into more than 20 polypeptides of molecular masses between 10 and 120 kDa. The irradiation of rats with a dose of 8 Gy led to changes in the polypeptide content of mitochondrial acid-soluble proteins in the postradiation period. It was found that the liver acid-soluble proteins of control and irradiated rats were able to form nucleoproteid complexes with DNA at the physiological NaCl concentration. It was shown that along with mitochondrial acid-soluble proteins, proteases were also released, their activity increased in the presence of DNA. Twenty four hours after irradiation of rats with 8 Gy, the activity of proteases cleaving mitochondrial acid-soluble proteins decreased. Probably, the acid-soluble proteins and DNA-activated proteases of mitochondria are involved in the regulation of the structural organization and functional activity of mitochondrial DNA.
Kutsyĭ
M P
MP
Guliaeva
N A
NA
Kuznetsova
E A
EA
Gaziev
A I
AI
D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D003627:Data Interpretation, Statistical; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays; D006657:Histones / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism / Q000528:radiation effects*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013997:Time Factors
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D006657:Histones; D024101:Mitochondrial Proteins; D010455:Peptides; D010447:Peptide Hydrolases