title: Quantification of silver-staining nucleolar organizer region in epithelial cells of tongue of mice after exposure to, or intake of, alcohol.
abstract: The purpose of the current study was to evaluate the effect of alcohol on the proliferative activity of epithelial cells in the lingual mucosa of mice by means of silver-staining nucleolar organizer region (AgNOR) count and area measurements. Forty-eight CF1 mice were separated into three groups. The test groups were submitted to topical exposure to, or intake of, 40% (volume/volume) ethyl alcohol. Biopsy specimens were collected from the middle third of the dorsal tongue at 0, 6, and 12 months, and samples were stained according to the AgNOR technique. Mean number and mean area of AgNOR per nucleus were calculated for 50 basal layer cells and 50 intermediate layer cells. Increases in mean number and mean area of AgNOR per nucleus in intermediate cells were observed at 12 months in the alcohol intake group (P < .05). Results showed that intake of 40% alcohol increased epithelial cell proliferation in the dorsal surface of lingual mucosa.
authors.affiliation: Department of Oral Histology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Vinicius C
authors.initials: VC
authors.lastname: Carrard
authors.forename: Manoel Sant'Ana
authors.initials: MS
authors.lastname: Filho
authors.forename: Pantelis V
authors.initials: PV
authors.lastname: Rados
authors.forename: Anna Cecília M
authors.initials: AC
authors.lastname: Chaves
authors.forename: Isabel da Silva
authors.initials: Ida S
authors.lastname: Lauxen
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D051379:Mice; D009061:Mouth Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D009697:Nucleolus Organizer Region / Q000737:chemistry / Q000187:drug effects*; D016622:Silver Staining / Q000379:methods; D014059:Tongue / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*
chemical_list: D000431:Ethanol