Characterization of a molt-inhibiting hormone (MIH) receptor in the Y-organ of the kuruma prawn, Marsupenaeus japonicus.
To characterize a receptor for molt-inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross-linking experiments using radiolabeled recombinant MIH ([(125)I]rMIH). The specific binding of [(125)I]rMIH was found in the membrane fraction of the Y-organ (K(d) = 4.76 x 10(-10) M and B(max) = 5.51 x 10(-12) M). Chemical cross-linking between [(125)I]rMIH and the membrane fraction of the Y-organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.
Asazuma
Hideaki
H
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Japan.
Nagata
Shinji
S
Katayama
Hidekazu
H
Ohira
Tsuyoshi
T
Nagasawa
Hiromichi
H
D000818:Animals; D004702:Endocrine Glands / Q000378:metabolism*; D007447:Invertebrate Hormones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology
D007447:Invertebrate Hormones; D009479:Neuropeptides; C012445:molt-inhibiting hormone