title: [Concentration and determination of strychnine alkaloid in biological fluids].
abstract: OBJECTIVE
  To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers.
  
  
  METHODS
  A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids.
  
  
  RESULTS
  the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit.
  
  
  CONCLUSIONS
  The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.
authors.affiliation: School of medicine, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lang-chong
authors.initials: LC
authors.lastname: He
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D012680:Sensitivity and Specificity; D013331:Strychnine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D000470:Alkaloids; D011108:Polymers; C083806:brucine; D013331:Strychnine