Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete.
An alkaliphilic actinomycete, strain YIM 80305(T), which was isolated from a muddy sample in Chaka salt lake, Qinghai Province of China, was characterized using a polyphasic approach. The isolate produced light-yellow substrate and yellow-white aerial mycelia on most tested media. Optimum pH for growth was 9.0-10.0 with scant growth at pH 7.0. Results showed that strain YIM 80305(T) was obligately Na(+)-dependent, and showed sensitivity to K(+). The DNA G + C content was 70.5 mol%. 16S rRNA gene sequence analysis together with these characteristics consistently assigned strain YIM 80305(T) to the genus Streptomyces. It formed a distinct clade based on analyses of the almost-complete and 120-nucleotide variable gamma region of the 16S rRNA gene. It could be differentiated by phenotypic and genotypic analysis from all the Streptomyces species whose names have been validly published. On the basis of polyphasic evidence, Streptomyces sodiiphilus sp. nov. is proposed. The type strain is YIM 80305(T) (= CCTCC AA 203015(T) = CIP 107975(T)).
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Zhang
Yong-Guang
YG
Research Centre of Industrial Microbiology, School of Biotechnology, Southern Yangtze University, Wuxi, 214036, China.|The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Zhang
Yu-Qin
YQ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Xu
Ping
P
New Drug R & D, North China Pharmaceutic Corp., Shijiazhuang, 050015, P. R. China.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006131:Growth Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011188:Potassium / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013302:Streptomyces / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006131:Growth Inhibitors; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D011188:Potassium