Transient reporter RNA assay: quantification of reporter gene mRNA during immediate early response in mammalian cells based on real-time reverse transcription polymerase chain reaction.
Ishihara
Hiroshi
H
Radiation Safety Research Center, Radiation Emergency Research Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan. ishihara@nirs.go.jp
Tanaka
Izumi
I
Ishihara
Fumiko
F
Suzuki
Keiko
K
Yoshino
Chieko
C
Cheeramakara
Cheerarattana
C
Wan
Hong
H
Akashi
Shingo
S
D000818:Animals; D002460:Cell Line; D017781:Genes, Immediate-Early; D017930:Genes, Reporter / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic
D010703:Phorbol Esters; D012333:RNA, Messenger; D008156:Luciferases