Chryseobacterium taichungense sp. nov., isolated from contaminated soil.
A bacterial strain (CC-TWGS1-8(T)) isolated from a tar-contaminated soil in Taiwan was studied in a detailed taxonomic study. The cells were Gram-negative, rod-shaped and non-spore-forming. Phylogenetic analyses using the 16S rRNA gene sequence of the strain clearly revealed an affiliation to the genus Chryseobacterium, the highest sequence similarities being to the type strain of Chryseobacterium indologenes (96.8 %), to Chryseobacterium gleum (96.8 %) and to Chryseobacterium joostei (96.4 %). The 16S rRNA gene sequence similarities to all other Chryseobacterium species were below 96 %. The major whole-cell fatty acids were 15 : 0 iso (35.4 %) and 17 : 0 iso 3OH (22.5 %). DNA-DNA hybridization values and the biochemical and chemotaxonomic properties demonstrate that strain CC-TWGS1-8(T) represents a novel species, for which the name Chryseobacterium taichungense sp. nov. is proposed. The type strain is CC-TWGS1-8(T) (= CCUG 50001(T) = CIP 108519(T)).
Shen
Fo-Ting
FT
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
Young
Chiu-Chung
CC
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Lai
Wei-An
WA
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Arun
A B
AB
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D045247:Chryseobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013624:Taiwan
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D005840:Gentian Violet