Characterization of neural cell types expressing peroxiredoxins in mouse brain.
The differential expression patterns of antioxidant enzymes observed in the brains of patients with neurodegenerative diseases suggest an important role for reactive oxygen species and antioxidant enzymes in neurodegeneration. The six mammalian peroxiredoxins (Prxs) comprise a novel family of anti-oxidative proteins that are widely distributed in most tissues, but few studies of Prx in brain tissue have been reported. The specific histology of the neural cell types in which Prxs are expressed is an important issue related to biological function and defense against oxidative stress in the brain. This study analyzed mouse brain neural cell types expressing Prx isoforms using single- or double-label immunohistochemical techniques. In neurons, immunoreactivity for Prx II-V was observed in the cytoplasm. In particular, Prx II was found in the habenular nuclei, and Prx III and V were found in the stratum lucidum of the hippocampus. Astrocytes and microglia were immunoreactive only for Prx VI and Prx I, respectively. Prx I and IV immunoreactivity was apparent in oligodendrocytes, where it was principally localized in the nuclei. The observed distribution of Prx isoforms in the mammalian brain may be indicative of their specific roles in their preferred neural cell types and subcellular locales. The results of this study will help in unraveling the physiological and pathophysiological roles of the different Prx isoforms in neural function.
Jin
Mei-Hua
MH
Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, 52, Oun-dong, Yusong-gu, Daejeon 305-333, South Korea.
Lee
Young-Ho
YH
Kim
Jin-Man
JM
Sun
Hu-Nan
HN
Moon
Eun-Yi
EY
Shong
Min Ho
MH
Kim
Sun-Uk
SU
Lee
Sang Ho
SH
Lee
Tae-Hoon
TH
Yu
Dae-Yeul
DY
Lee
Dong-Seok
DS
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D017628:Microglia / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism; D010544:Peroxidases / Q000096:biosynthesis*; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins
D007527:Isoenzymes; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins