title: A zinc finger protein TZF is a novel corepressor of androgen receptor.
abstract: Steroid hormones control the transcriptional activity of target genes mediated by intracellular nuclear receptors, and these transcriptional activities are modulated by the combination with coactivators and corepressors. We found in this study that testicular zinc finger protein (TZF) that was a nuclear protein with a zinc finger motif of the Cys2-His2 type was a novel corepressor of androgen receptor (AR). Fusion protein with green fluorescence protein GFP formed the specific foci in nuclei and TZF-dependent foci were located close to the splicing factor compartment. In addition, TZF was recruited into AR subnuclear foci after the treatment of dihydrotestosterone. Furthermore, we revealed that TZF bound to the activation function-1 (AF-1) domain (N-terminal transactivating domain) of AR protein. Transient over-expression of TZF in COS-7 cells or LNCaP human prostatic cancer cell resulted in decreased AR activity in a ligand-dependent fashion. Moreover, a transcriptional corepressor N-CoR additively decreased the transcriptional activity of AR with TZF. These findings suggest that TZF might be a novel corepressor of AR.
authors.affiliation: Department of Biological Sciences, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Hisaya
authors.initials: H
authors.lastname: Kawate
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Takayanagi
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ohshima
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Tao
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hagiwara
mesh_terms: D059002:Androgen Receptor Antagonists; D000818:Animals; D019556:COS Cells; D011485:Protein Binding; D012326:RNA Splicing; D011944:Receptors, Androgen / Q000378:metabolism / Q000502:physiology; D012097:Repressor Proteins / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation / Q000502:physiology; D016335:Zinc Fingers
chemical_list: D059002:Androgen Receptor Antagonists; D011944:Receptors, Androgen; D012097:Repressor Proteins