Signaling pathways involved in tumor necrosis factor alpha-induced upregulation of the taurine transporter in Caco-2 cells.
We have previously demonstrated that the taurine uptake and transporter (TAUT) mRNA expression were upregulated by tumor necrosis factor alpha (TNF-alpha) in Caco-2 cells. In this present study, the signaling molecules related to this upregulation were investigated. Pyrrolidine dithiocarbamate, a nuclear factor kappaB (NF-kappaB) inhibitor, repressed the upregulation of taurine uptake and TAUT mRNA expression. A reporter assay revealed that TNF-alpha-induced TAUT transcriptional activity through the NF-kappaB consensus-like sequence in the human TAUT promoter region. An electrophoretic mobility shift assay showed that NF-kappaB could bind to the NF-kappaB consensus-like sequence. The anti-TNF receptor 1 (TNFR1) antibody, but not the TNF receptor 2 (TNFR2) antibody, repressed this upregulation.
Mochizuki
Tetsunosuke
T
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa37021@mail.ecc.u-tokyo.ac.jp
Satsu
Hideo
H
Shimizu
Makoto
M
D001483:Base Sequence; D018938:Caco-2 Cells; D016384:Consensus Sequence / Q000235:genetics; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013654:Taurine / Q000494:pharmacology; D013859:Thiocarbamates / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*; D000099247:Taurine Transporters
D020672:I-kappa B Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B; D011392:Proline; D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D013654:Taurine; D013859:Thiocarbamates; D014409:Tumor Necrosis Factor-alpha; D000099247:Taurine Transporters; C000606204:NFKBIA protein, human; C066229:prolinedithiocarbamate