Stimulation of midgut stem cell proliferation and differentiation by insect hormones and peptides.
Stem cells derived from midguts of the caterpillar, Spodoptera littoralis, can be induced to multiply and differentiate in vitro. Ecdysone (E) and 20-hydroxyecdysone (20E) had a concentration-dependent effect: E was more active in cell proliferation and 20E in differentiation. Ecdysteroid receptors in midgut stem cell nuclei were stained with the antibody 9B9. In addition, alpha-arylphorin and four midgut differentiation factors (MDF) specifically stimulated proliferation and differentiation of stem cells, respectively. The activity of a panel of peptide growth factors and hormones on growth and metamorphosis of the insect midgut is discussed.
Smagghe
Guy
G
Laboratory of Agrozoology, Ghent University, Belgium. guy.smagghe@ugent.be
Vanhassel
Wendy
W
Moeremans
Clarina
C
De Wilde
Daan
D
Goto
Shintaro
S
Loeb
Marcia J
MJ
Blackburn
Michael B
MB
Hakim
Raziel S
RS
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004440:Ecdysone / Q000502:physiology; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D007301:Insect Hormones / Q000502:physiology*; D019476:Insect Proteins / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D018411:Spodoptera / Q000187:drug effects / Q000254:growth & development*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
D007301:Insect Hormones; D019476:Insect Proteins; D010455:Peptides; D004440:Ecdysone