[Sequences and their polymorphisms of swine MHC-DQB, DRB proximal promoter region].
The primers, RF/RR and QF1/QR,were designed based on the homologous region of swine MHC-DQB and DRB exon1 sequences and HLA-DQB and HLA-DRB sequences. The 5' proximal promoter region (URR) nucleotide sequences of swine MHC-DQB and DRB were firstly obtained by PCR, cloning and sequencing techniques. There exist highly conserved sequence motifs including W, X, Y, CCAAT, and TATA-like boxes in the obtained sequences,showing the same organization of the conserved regulatory elements as in other species. 12 DRB-URR and 14 DQB-URR alleles were found by PCR-SSCP in 313 pigs. The aligned results of these alleles showed that there exist abundant polymorphism sites in both Porcine MHC-DQB and DRB proximal promoter region.
X U
Ru-Hai
RH
College of Animal Science & Technology, China Agricuutural Univeisity, Beijing 100094, China.
Wang
Kun
K
Sun
Dong-Xiao
DX
Ding
Xiang-Dong
XD
Liu
Pei-Qiong
PQ
Zhang
Qin
Q
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*; D016385:TATA Box
D000949:Histocompatibility Antigens Class II