Towards a molecular code for individuality in the absence of MHC: screening for individually variable genes in the urochordate Ciona intestinalis.
Urochordates possess several well described allorecognitions systems, the molecular nature of those is not yet understood. A prerequisite for any self-/nonself discimination system is the presence of a group of highly variable molecules, which should vary between individuals. Using suppression subtractive hybridisation (SSH) we surveyed Ciona intestinalis for individually variable genes. Our search so far identified two genes, ciS7 and ciMETA2, which both display an unexpected high degree of intra- and interindividual variability, code for secreted proteins, and contain multiple domains suitable for protein-protein interactions. The possible role of these molecules in allorecognition is discussed.
Khalturin
Konstantin
K
Zoological Institute, Christian-Albrechts-University Kiel, Olshausenstrasse 40, 24098 Kiel Germany. kkhalturin@zoologie.uni-kiel.de
Kürn
Ulrich
U
Pinnow
Nicole
N
Bosch
Thomas C G
TC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002938:Ciona intestinalis / Q000235:genetics* / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011506:Proteins