title: Mouse syngenic in vitro blood-brain barrier model: a new tool to examine inflammatory events in cerebral endothelium.
abstract: Although cerebral endothelium disturbance is commonly observed in central nervous system (CNS) inflammatory pathologies, neither the cause of this phenomenon nor the effective participation of blood-brain barrier (BBB) in such diseases are well established. Observations were mostly made in vivo using mouse models of chronic inflammation. This paper presents a new mouse in vitro model suitable for the study of underlying mechanistic events touching BBB functions during CNS inflammatory disturbances. This model consists of a coculture with both primary cell types isolated from mice. Mouse brain capillary endothelial cell (MBCEC)s coming from brain capillaries are in culture with their in vivo partners and form differentiated monolayers that retain endothelial markers and numerous phenotypic properties of in vivo cerebral endothelium, such as: (1) peripheral distribution of tight junction proteins (occludin, claudin-5, claudin-3 and JAM-1); (2) high trans-endothelium electrical resistance value; (3) attenuated paracellular flux of sucrose and inulin; (4) P-gp expression; (5) no MECA-32 expression. Furthermore, this endothelium expresses cell adhesion molecules described in vivo and shows intracellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 upregulation under lipopolysaccharide-treatment. Therefore, this well-differentiated model using autologous cells appears as a suitable support to reconstitute pathological in vitro BBB model.
authors.affiliation: EA 2465, Unité mixte Université d'Artois, Institut Pasteur de Lille, Lens Cedex, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Coisne
authors.forename: Lucie
authors.initials: L
authors.lastname: Dehouck
authors.forename: Christelle
authors.initials: C
authors.lastname: Faveeuw
authors.forename: Yannick
authors.initials: Y
authors.lastname: Delplace
authors.forename: Florence
authors.initials: F
authors.lastname: Miller
authors.forename: Christophe
authors.initials: C
authors.lastname: Landry
authors.forename: Céline
authors.initials: C
authors.lastname: Morissette
authors.forename: Laurence
authors.initials: L
authors.lastname: Fenart
authors.forename: Romeo
authors.initials: R
authors.lastname: Cecchelli
authors.forename: Patrick
authors.initials: P
authors.lastname: Tremblay
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Dehouck
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D002196:Capillaries; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000502:physiology*; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D007249:Inflammation / Q000503:physiopathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008833:Microcirculation / Q000502:physiology; D023421:Models, Animal; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides