The effects of short-term training on platelet functions and total antioxidant capacity in rats.
The purpose of the present study was to investigate the effect of short-term endurance training on plasma total antioxidant status (TAS) and on in vitro platelet aggregation and ATP release. Blood samples were collected from the abdominal aorta of rats following short-term treadmill exercise (25 m/min, 0 % grade, 30 min) for three consecutive days, as well as in non-exercised control group. Platelet aggregation and platelet ATP release were evaluated by impedance and bioluminescence techniques, respectively. Plasma TAS was measured spectrophotometrically. Plasma TAS was higher and ADP-induced platelet ATP release was lower in the short-term training group with respect to the control group (p<0.001). Significant negative correlation (r = -0.56, p<0.05) was found between plasma TAS and ADP-induced platelet ATP release. Neither ADP- and collagen-induced maximum aggregation rate nor collagen-induced platelet ATP release were significantly different between the groups. According to these results, short-term training caused an alteration in platelet functions limited to the secretion response, which may be related to the oxidant/antioxidant balance changes favoring the antioxidants. The improved plasma total antioxidant capacity was possibly sufficient to prevent exercise-induced oxidative damage, and the adaptive response of platelets might be associated with enhanced antioxidant status.
Ficicilar
H
H
Department of Physiology, Ankara University, School of Medicine, Ankara, Turkey.
Zergeroglu
A M
AM
Ersoz
G
G
Erdogan
A
A
Ozdemir
S
S
Tekin
D
D
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010949:Plasma / Q000378:metabolism*; D010974:Platelet Aggregation / Q000502:physiology; D010976:Platelet Count; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants