In vitro transcription of a whole gene on a surface-coupled template.
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.
Steffen
Jenny
J
Fraunhofer Institute for Biomedical Engineering, Dept. Molecular Bioanalytics and Bioelectronics, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany. jenny.steffen@ibmt.fhg.de
von Nickisch-Rosenegk
Markus
M
Bier
Frank F
FF
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005898:Glass / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D008957:Models, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013499:Surface Properties; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004279:DNA, Viral; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins