[Morphological and molecular biological peculiarities of the experimental autoimmune prostatitis rat model].
OBJECTIVE
To observe the morphological and molecular biological peculiarities of the experimental autoimmune prostatitis (EAP) rat model made by SC purified prostate protein twice with immune adjuvant.


METHODS
Male rats were intradermally immunized with a saline extract of male rat prostate glands (RPG) in Freund's complete adjuvant (FCA) and Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. at the 0 and 30th day, and the concentrations of the extract were respectively 5 mg/ml, 10 mg/ml and 15 mg/ml. At the 45th day, the rats were sacrificed and the morphological and molecular biological changes of the prostate specimens were observed to determine the effective concentration of RPG for a successful model.


RESULTS
The expression of inflammation genes such as TNF-alpha, IL-1beta, IL-2 and iNOS obviously increased in the high-dosage model group; LM, EM and in situ hybridization revealed appearant chronic inflammation response, but this was not the case in the other two dosage groups.


CONCLUSIONS
15 mg/ml RPG mixed with FCA (1:1) 1.0 ml SC with Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. was an effective dosage for the successful model in our experiment.
Zhou
Xiao-hui
XH
Zhejiang University School of Pharmaceutical Sciences, Hangzhou, Zhejiang 310031, China. zhxh@zju.edu.cn
Han
Lei
L
Zhou
Zhi-heng
ZH
Liu
Zhong-de
ZD
Yang
Ji-xiang
JX
Lv
Yan-wei
YW
You
Chun-lai
CL
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000008:administration & dosage; D004195:Disease Models, Animal; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D008297:Male; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D011472:Prostatitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D015721:Diphtheria-Tetanus-Pertussis Vaccine; D011506:Proteins; D005620:Freund's Adjuvant