title: Retention of nativelike conformation by proteins embedded in high external electric fields.
abstract: In this Communication, we show that proteins embedded in high external electric fields are capable of retaining a nativelike fold pattern. We have tested the metalloprotein azurin, immobilized onto SiO2 substrates in air with proper electrode configuration, by applying static fields up to 10(6)-10(7) Vm. The effects on the conformational properties of protein molecules have been determined by means of intrinsic fluorescence measurements. Experimental results indicate that no significant field-induced conformational alteration occurs. Such results are also discussed and supported by theoretical predictions of the inner protein fields.
authors.affiliation: National Nanotechnology Laboratories of INFM, University of Lecce, Via per Arnesano 73100 Lecce, Italy.
authors.forename: P P
authors.initials: PP
authors.lastname: Pompa
authors.forename: A
authors.initials: A
authors.lastname: Bramanti
authors.forename: G
authors.initials: G
authors.lastname: Maruccio
authors.forename: R
authors.initials: R
authors.lastname: Cingolani
authors.forename: F
authors.initials: F
authors.lastname: De Rienzo
authors.forename: S
authors.initials: S
authors.lastname: Corni
authors.forename: R
authors.initials: R
authors.lastname: Di Felice
authors.forename: R
authors.initials: R
authors.lastname: Rinaldi