Polyhydroxylated steroidal constituents from the fresh rhizomes of Tupistra yunnanensis.
Six new polyhydroxylated steroidal saponins, tupistrosides A-F (1-6), together with nine known steroids, were isolated from the fresh rhizomes of Tupistra yunnanensis. Their structures were elucidated to be (25S)-1beta,4beta,5beta-trihydroxy-spirostane-3beta-yl O-alpha-l-arabinopyranoside (1), 1beta,24beta-dihydroxy-spirost-5,25(27)-dien-3alpha-yl O-beta-d-glucopyranoside (2), (22S,25S)-1alpha,2beta,3alpha,5alpha-tetrahydroxy-furo-spirostane-26-yl O-beta-d-glucopyranoside (3), 1beta,3alpha,22 xi-trihydroxy-furost-5,25(27)-dien-26-yl O-beta-d-glucopyranoside (4), 26-O-beta-d-glucopyranosyl-1beta,22-dihydroxy-furost-5-en-3alpha-yl O-beta-d-glucopyranoside (5) and 22-methoxy-1beta,2beta,3beta,4beta,5beta,7alpha-hexahydroxy-furost-25(27)-en-6-one-26-yl O-beta-d-glucopyranoside (6), respectively, by means of spectroscopic analysis and the results of acid hydrolysis.
Yang
Qing-Xiong
QX
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
Zhang
Ying-Jun
YJ
Li
Hai-Zhou
HZ
Yang
Chong-Ren
CR
D003525:Cyclosteroids / Q000302:isolation & purification*; D006851:Hydrochloric Acid; D006868:Hydrolysis; D019668:Liliaceae / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification*; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*
D003525:Cyclosteroids; D010936:Plant Extracts; D012503:Saponins; D006851:Hydrochloric Acid