title: Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation.
abstract: It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Hayashi
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Ogura
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Harry
authors.forename: Naotake
authors.initials: N
authors.lastname: Ogasawara
authors.forename: Shigeki
authors.initials: S
authors.lastname: Moriya
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005434:Flow Cytometry; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C103076:DnaX protein, Bacteria; D015534:Trans-Activators; C045264:replication initiator protein; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; C083980:dnaN protein, Bacteria; D004265:DNA Helicases