Anti-oxidants reduce the acute adverse effects of residual oil fly ash on the frog palate mucociliary epithelium.
There is evidence indicating that oxidants play a pivotal role in determining air pollution-dependent lung injury. In the present study we explored the role of oxidants present in ambient particles in causing damage to the mucociliary epithelium. We explored the protective effects of pretreatment with three substances (n-propyl gallate, DL-alpha-tocopherol acetate, and EDTA) on the frog palate exposed to residual oil fly ash (ROFA). The parameters analyzed were mucociliary transport (MCT) and ciliary beating frequency (CBF) after 0, 10, 20, 30, 60, and 120 min of exposure. MCT was decreased significantly by ROFA (P < 0.001), with a significant interaction effect (P = 0.02) between the duration of exposure and treatment with antioxidants. The inhibitory effects on MCT of the substances tested were significantly different (P = 0.002); vitamin E was similar to control (Ringer) and different from all other groups. CBF showed no significant effect of duration of exposure (P = 0.465), but a significant interaction between duration of exposure and treatments was observed (P = 0.011). Significant differences were detected among treatments (P < 0.001), with ROFA and n-propyl gallate at concentrations of 50 microM presenting a short-lived increase in CBF, which was not observed in the remaining groups. The results showed that both MCT and CBF were affected within a short period (100 min) of exposure to ROFA and that the presence of antioxidant substances, such as vitamin E (4 mg/mL) and n-propyl gallate (300 microM), protected against the mucociliary impairment induced by ROFA on the frog palate.
Carvalho-Oliveira
Regiani
R
Laboratory of Experimental Air Pollution, Department of Pathology, University of São Paulo, Av. Dr. Arnaldo 455, SP CEP01246-903 São Paulo, Brazil.
Saiki
Mitiko
M
Pires-Neto
Ruy C
RC
Lorenzi-Filho
Geraldo
G
Macchione
Mariangela
M
Saldiva
Paulo H N
PH
D000208:Acute Disease; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001001:Anura; D002244:Carbon / Q000633:toxicity*; D060729:Coal Ash; D004347:Drug Interactions; D004492:Edetic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects* / Q000378:metabolism; D019570:Inhalation Exposure; D009079:Mucociliary Clearance; D010159:Palate / Q000166:cytology / Q000187:drug effects*; D052638:Particulate Matter; D011435:Propyl Gallate / Q000494:pharmacology; D013997:Time Factors; D024505:Tocopherols; D024502:alpha-Tocopherol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000393:Air Pollutants; D000975:Antioxidants; D060729:Coal Ash; D052638:Particulate Matter; D002244:Carbon; D011435:Propyl Gallate; D004492:Edetic Acid; D024502:alpha-Tocopherol; D024505:Tocopherols