Elevated levels of WT1 transcripts in bone marrow harvests are associated with a high relapse risk in patients autografted for acute myeloid leukaemia.
Relapse postautograft in acute myeloid leukaemia (AML), may in part arise from leukaemia cells present in the bone marrow (BM) inoculum, and the level of minimal residual disease (MRD) in BM harvests used for autografting may therefore be clinically important. We have used the WT1 transcript as a marker of MRD, which was quantitated by RQ-PCR, in the BM harvests of 24 patients receiving an ABMT for AML. ABL was used as a control gene with WT1 level being normalised to 10(5) copies of ABL per sample. Median WT1 level was 651 copies (range=113-32 700) for the 13 patients with relapse-free survival (RFS) of less than 5 years, and 174 (range=0-1900) for patients with RFS of over 5 years postautograft (P<0.04). The RFS was 10.5 months for patients with WT1 level of >2000 copies (n=5), and has not yet been reached for patients with WT1 level<2000 (n=21), at a median follow-up of 92 months (P<0.05). We show that elevated levels of MRD in BM harvests are associated with a higher relapse risk in patients autografted for AML.
Osborne
D
D
University Department of Haematology, Manchester Royal Infirmary, Manchester, UK.
Frost
L
L
Tobal
K
K
Liu Yin
J A
JA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001781:Blood Component Removal; D001853:Bone Marrow / Q000737:chemistry / Q000473:pathology; D016026:Bone Marrow Transplantation; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007951:Leukemia, Myeloid / Q000175:diagnosis / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis*; D012008:Recurrence; D012306:Risk; D014182:Transplantation, Autologous; D025721:WT1 Proteins / Q000235:genetics*
D012333:RNA, Messenger; D025721:WT1 Proteins