title: Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness.
abstract: We have previously demonstrated a new cell manipulation technology by using an atomic force microscope (AFM) and ultrathin needles, named nanoneedles. The nanoneedle is an AFM tip etched by a focused ion beam (FIB) and is sharpened from 200 to 800 nm in diameter. In this study, we have evaluated the proper diameter of a needle required for insertion into human cells over a long period without causing cell death, and achieved highly efficient gene expression method for human cells using a nanoneedle and an AFM.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
authors.forename: SungWoong
authors.initials: S
authors.lastname: Han
authors.forename: Chikashi
authors.initials: C
authors.lastname: Nakamura
authors.forename: Ikuo
authors.initials: I
authors.lastname: Obataya
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nakamura
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
mesh_terms: D002455:Cell Division; D002460:Cell Line; D048430:Cell Shape; D002470:Cell Survival; D015870:Gene Expression; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force / Q000295:instrumentation; D018613:Microscopy, Confocal; D009339:Needles; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins