title: Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements.
abstract: Galpha(12/13) have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Galpha(12) and Galpha(13) signaling by overexpressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Galpha(12/13) are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, Galpha(12/13) function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
authors.affiliation: Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Lin
authors.forename: Diane S
authors.initials: DS
authors.lastname: Sepich
authors.forename: Songhai
authors.initials: S
authors.lastname: Chen
authors.forename: Jacek
authors.initials: J
authors.lastname: Topczewski
authors.forename: Chunyue
authors.initials: C
authors.lastname: Yin
authors.forename: Lilianna
authors.initials: L
authors.lastname: Solnica-Krezel
authors.forename: Heidi
authors.initials: H
authors.lastname: Hamm
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D044365:GTP-Binding Protein alpha Subunits, G12-G13 / Q000235:genetics / Q000378:metabolism*; D005775:Gastrula / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D043562:Receptors, G-Protein-Coupled; D051153:Wnt Proteins; D044365:GTP-Binding Protein alpha Subunits, G12-G13