Rational strategy for the production of new crude lipases from Candida rugosa.
Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis.
de María
Pablo Domínguez
PD
Grupo de Biotransformaciones, Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad Complutense Madrid, Plaza de Ramón y Cajal, s/n. Ciudad Universitaria, 28040 Madrid, Spain.
Sánchez-Montero
José M
JM
Alcántara
Andrés R
AR
Valero
Francisco
F
Sinisterra
José V
JV
D002175:Candida / Q000201:enzymology*; D007851:Dodecanol / Q000378:metabolism; D004790:Enzyme Induction; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D000074242:Sunflower Oil
D007527:Isoenzymes; D009829:Oleic Acids; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D007851:Dodecanol; D019301:Oleic Acid; C068800:heptyl oleate; D008049:Lipase; D005990:Glycerol