[Modulation of cell proliferation and collagen synthesis of porcine Tenon's fibroblasts by extracts of lens and vitreous with treatment of dexamethasone].
OBJECTIVE
To demonstrate the effects of lens extract (LE), vitreous extract (VE) and dexamethasone on the modulating of the proliferation and collagen synthesis of Tenon's capsule fibroblast.


METHODS
Cell proliferation and collagen synthesis of Tenon's fibroblast were measured using MTT and reverse transcription polymerase chain reaction techniques in the presence or absence of LE, VE, or dexamethasone, respectively.


RESULTS
The growth of Tenon's fibroblasts was stimulated by LE and VE in dose and time-dependent manners. Dexamethasone significantly inhibited the proliferation of the cells either in the presence or absence of LE and VE. Similarly, LE and VE significantly stimulated the collagen synthesis of the cells. However, dexamethasone showed no significant effect on the LE or VE-enhanced collagen synthesis of the cells.


CONCLUSIONS
These results suggested that there might be some ingredients in LE or VE that could stimulate the cell proliferation and collagen synthesis of Tenon's capsule fibroblasts. These ingredients, if released, may affect the success rate of filtration surgery for glaucoma. The application of glucocorticoid itself might not be sufficient to retard the scar formation following the filtering surgery in refractory glaucoma after cataract surgery or vitrectomy.
Xu
Dong-dong
DD
Department of Ophthalmology, Peking Union Medical College Hospital, Eye Research Center, Chinese Academy of Medical Sciences, Beijing 100730, China. guaji@yahoo.com
Li
Ruo-mei
RM
Lian
Lian
L
Liu
Xi-pu
XP
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D003228:Conjunctiva / Q000166:cytology; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000737:chemistry*; D013552:Swine; D014822:Vitreous Body / Q000737:chemistry*
D005938:Glucocorticoids; D003907:Dexamethasone; D003094:Collagen