A comparison of feeding efficiency and swimming ability of Daphnia magna exposed to cypermethrin.
Microcosm and mesocosm studies evidence that pyrethroid insecticides may have a severe effect on zooplankton populations. The effect may cascade to phytoplankton communities and thus worsen the impact of eutrophication and algal blooms. In natural freshwater systems, pyrethroids are usually only detectable during the first 24 h after application to adjacent areas, a period too short for mesocosm and microcosm studies to reveal potential effects. In this study we compare the effects of environmentally realistic concentrations of the pyrethroid cypermethrin on: (i) the swimming ability of Daphnia magna; (ii) the feeding efficiency, measured as the content of chlorophyll pigments in the gut; and (iii) the total body carbohydrate content. The latter two were measured using a newly developed high performance planar chromatography (HPPC) system. Sublethal effects on the gut content of chlorophyll pigments, carbohydrate substances and the swimming ability of D. magna were observed at nominal concentrations between 0.05 and 0.6 microg cypermethrinL(-1), which lies within the concentration range occurring in freshwater systems after pesticide application. In addition, the content of chlorophyll pigments in the gut was significantly reduced (>50%) after only 6 h of exposure to 0.1 microg cypermethrinL(-1). Most of the D. magna had recovered 3 days after exposure doses lower than 0.2 microg cypermethrinL(-1). We conclude that HPPC analysis of the gut content of chlorophyll pigments was the most sensitive endpoint of our study due to its capacity to detect significant reductions in feeding within hours of exposure to environmentally realistic concentrations of cypermethrin.
Christensen
Birthe Thordahl
BT
Department of Freshwater Ecology, National Environmental Research Institute, Vejlsøvej 25, P.O. Box 314, DK-8600 Silkeborg, Denmark.
Lauridsen
Torben L
TL
Ravn
Helle Weber
HW
Bayley
Mark
M
D000704:Analysis of Variance; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002734:Chlorophyll / Q000378:metabolism / Q000493:pharmacokinetics; D002845:Chromatography / Q000379:methods; D003621:Daphnia / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D008124:Locomotion / Q000187:drug effects*; D011722:Pyrethrins / Q000633:toxicity*
D007306:Insecticides; D011722:Pyrethrins; D002734:Chlorophyll; C017160:cypermethrin