[Preparation and identification of monoclonal antibody against heterogeneous nuclear ribonucleoprotein B1].
OBJECTIVE
To establish anti-heterogeneous nuclear ribonucleoprotein B1 monoclonal antibody cell line and to identify immunological activity.


METHODS
BALB/c mice were immunized with specific hnRNPB1 polypeptide by multiple-place hypodermic injection. The splenic cells of the mice were fused with SP2/0 mouse myeloma cells. The hybridoma culture supernants were screened by indirect ELISA with artificial synthetic peptide.


RESULTS
Two hybridoma cell lines against hnRNPB1 were successfully obtained; the titers of antibody in ascites were 1: 1.8 x 10(5). The McAb showed the character of no cross reactions. Immunohistochemical analysis demonstrated that the antibody could bind with hnRNPB1 specifically.


CONCLUSIONS
The McAb prepared so by this laboratory is of good specificity and sensitivity.
Chen
Ming-yong
MY
Respiratory Department, West China Hospital, Sichuan University, Chengdu, China.
Li
Wei-min
WM
Xu
Dan
D
Wei
Da-peng
DP
Chen
Wen-bin
WB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D016601:RNA-Binding Proteins