Using Dirichlet tessellation to help estimate microbial biomass concentrations.
Dirichlet tessellation was applied to estimate microbial concentrations from microscope well slides. The use of microscopy/Dirichlet tessellation to quantify biomass was illustrated with two species of morphologically distinct cyanobacteria (Microcystis aeruginosa and Anabaena flos aquae), and validated empirically by comparison with chlorophyll a and Coulter count analyses. Biomass estimates obtained by microscopy/Dirichlet tessellation were significantly correlated with the results obtained from chlorophyll a and Coulter analyses.
Dugan
Nicholas R
NR
United States Environmental Protection Agency, Water Supply and Water Resources Division, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA. dugan.nicholas@epa.gov
D000465:Algorithms; D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D015169:Colony Count, Microbial / Q000379:methods; D046869:Dolichospermum flos-aquae / Q000254:growth & development*; D007091:Image Processing, Computer-Assisted; D046931:Microcystis / Q000254:growth & development*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D018709:Statistics, Nonparametric
D002734:Chlorophyll; D000077194:Chlorophyll A