Human embryonic stem cell lines derived from the Chinese population.
Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.
Fang
Zhen Fu
ZF
Center for Developmental Biology, Xinhua Hospital, Shanghai Second Medical University, Shanghai, China.
Jin
Fan
F
Gai
Hui
H
Chen
Ying
Y
Wu
Li
L
Liu
Ai Lian
AL
Chen
Bin
B
Sheng
Hui Zhen
HZ
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D002681:China; D019976:Cloning, Organism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D053652:Nuclear Transfer Techniques; D044382:Population Groups; D013724:Teratoma / Q000378:metabolism / Q000473:pathology
D015415:Biomarkers; D006680:HLA Antigens