DNA mismatch detection by resonance energy transfer between ruthenium(II) and osmium(II) tris(2,2'-bipyridyl) chromophores.
Octahedral tris-chelate complexes [M(II)(bpy)(3)](2+) (M = Ru or Os, bpy = 2,2'-bipyridyl), covalently attached to the 3'- and 5'-phosphates of two oligonucleotides, are juxtaposed when hybridized contiguously to a fully complementary DNA target. Visible metal-to-ligand charge-transfer (MLCT) excitation of the [Ru(II)(bpy)(3)](2+) unit leads to resonance energy transfer to the MLCT state of the [Os(II)(bpy)(3)](2+) moiety, with the energy transfer efficiency depending on the degree of hybridization. The extent of attenuation of the intense red luminescence from the Ru(II) chromophore hence allows highly sensitive structural probing of the assembly and constitutes a novel approach to DNA sensing which is capable of detecting mutations.
Bichenkova
Elena V
EV
Wolfson Centre for Rational Design of Molecular Diagnostics, School of Pharmacy and Pharmaceutical Sciences, and School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, UK.
Yu
Xuan
X
Bhadra
Pranab
P
Heissigerova
Helena
H
Pope
Simon J A
SJ
Coe
Benjamin J
BJ
Faulkner
Stephen
S
Douglas
Kenneth T
KT
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004735:Energy Transfer; D008163:Luminescent Measurements; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009942:Organometallic Compounds / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*
D009942:Organometallic Compounds; D009992:Osmium; D012428:Ruthenium; D004247:DNA