title: Liver recipients harbouring anti-donor preformed lymphocytotoxic antibodies exhibit a poor allograft survival at the first year after transplantation: experience of one centre.
abstract: In this retrospective study, we analyzed the effect of the presence of anti-donor preformed alloantibodies in 268 liver allograft transplants. Crossmatches were performed by complement-dependent cytotoxicity (CDC) assay and HLA antibody screening by flow cytometry (FlowPRA). Positive anti-donor crossmatch was detected in 5.2% of transplants. Acute rejection frequency in +CDC crossmatch patients was not different from that observed in -CDC crossmatch patients. None of the patients transplanted with +CDC crossmatch developed chronic rejection, but they showed a significantly lower allograft survival rate, and the majority of them had allograft failures before the end of the first post-transplant year, mainly within the 3 first months. Indeed, positive FlowPRA determination was concordant with data from the CDC assay. In conclusion, these findings show a direct correlation between the presence of anti-donor preformed antibodies and a poor allograft survival in liver transplant.
authors.affiliation: Immunology Service, University Hospital Virgen de la Arrixaca, Murcia 30120, Spain. manuel.muro@carm.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Muro
authors.forename: Luis
authors.initials: L
authors.lastname: Marin
authors.forename: Manuel
authors.initials: M
authors.lastname: Miras
authors.forename: Rosa
authors.initials: R
authors.lastname: Moya-Quiles
authors.forename: Alfredo
authors.initials: A
authors.lastname: Minguela
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Bueno
authors.forename: Juan
authors.initials: J
authors.lastname: Bermejo
authors.forename: Ricardo
authors.initials: R
authors.lastname: Robles
authors.forename: Pablo
authors.initials: P
authors.lastname: Ramírez
authors.forename: Ana
authors.initials: A
authors.lastname: García-Alonso
authors.forename: Pascual
authors.initials: P
authors.lastname: Parrilla
authors.forename: María R
authors.initials: MR
authors.lastname: Alvarez-López
mesh_terms: D000961:Antilymphocyte Serum / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000097:blood* / Q000401:mortality; D006085:Graft Survival; D006801:Humans; D008107:Liver Diseases / Q000097:blood / Q000628:therapy*; D016031:Liver Transplantation / Q000401:mortality; D008297:Male; D011379:Prognosis; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D000961:Antilymphocyte Serum; D015415:Biomarkers