title: Antibody responses to recombinant protein fragments of the major outer membrane protein and polymorphic outer membrane protein POMP90 in Chlamydophila abortus-infected pregnant sheep.
abstract: Chlamydophila abortus is one of the major causes of infectious abortion in pregnant sheep (enzootic abortion of ewes or EAE) worldwide. Organisms shed in infected placentas and uterine discharges at lambing time are the main sources of environmental contamination, responsible for transmission to susceptible animals and possible human contacts. In the present study, a recently developed test, based on a recombinant fragment of the polymorphic outer membrane protein POMP90 (rOMP90-4 indirect enzyme-linked immunosorbent assay [iELISA]) and one based on the variable segment 2 (VS2) region of the major outer membrane protein (MOMP) (MOMP VS2 iELISA) were compared using sera from C. abortus-infected ewes at different stages throughout pregnancy. The rOMP90 iELISA detected antibody much earlier in pregnancy than the MOMP iELISA, which, like the complement fixation test, detected antibody only at the time of abortion or lambing. No anti-MOMP antibody response could be detected in three of seven experimentally infected ewes. Furthermore, the rOMP90 iELISA detected antibody in an animal that seroconverted during the course of the study, which the MOMP iELISA failed to detect. Overall, the results show that the rOMP90-4 iELISA is considerably more sensitive than the MOMP VS2 iELISA for identifying animals infected with C. abortus. Earlier detection of infection will allow appropriate control measures to be taken to reduce environmental contamination, thus limiting the spread of infection, financial losses, and the possible risks of zoonotic transmission to humans.
authors.affiliation: Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik EH26 0PZ, United Kingdom. David.Longbottom@mri.sari.ac.uk
authors.forename: Morag
authors.initials: M
authors.lastname: Livingstone
authors.forename: Gary
authors.initials: G
authors.lastname: Entrican
authors.forename: Sean
authors.initials: S
authors.lastname: Wattegedera
authors.forename: David
authors.initials: D
authors.lastname: Buxton
authors.forename: Iain J
authors.initials: IJ
authors.lastname: McKendrick
authors.forename: David
authors.initials: D
authors.lastname: Longbottom
mesh_terms: D000034:Abortion, Veterinary / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007075:Immunoglobulin M / Q000097:blood; D011247:Pregnancy; D011994:Recombinant Proteins / Q000276:immunology; D012756:Sheep / Q000097:blood; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D011994:Recombinant Proteins