Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody.
Araliaceous species containing ginsenosides, the major active component in Panax species, were surveyed using ELISA and eastern blotting with anti-ginsenoside Rb1 monoclonal antibody. Immunoassay-guided fractionation of the methanol-soluble extracts of Araliaceous species led to the isolation of a known compound in the bark of Kalopanax pictus Nakai. The known compound was identified as ginsenoside Rb1 by spectroscopic methods and by comparison with an authentic sample. The result provided evidence that a combination of two immunoassays using monoclonal antibodies against ginsenosides is a powerful means of surveying new ginsenoside resources.
Tanaka
Hiroyuki
H
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Fukuda
Noriko
N
Yahara
Shoji
S
Isoda
Susumu
S
Yuan
Chun-Su
CS
Shoyama
Yukihiro
Y
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D036145:Ginsenosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D036882:Kalopanax / Q000737:chemistry*; D008517:Phytotherapy
D000911:Antibodies, Monoclonal; D036145:Ginsenosides; C442759:ginsenoside Rb1