title: Commuting between Golgi cisternae--mind the GAP!
abstract: Intracellular transport has remained central to cell biology now for more than 40 years. Despite this, we still lack an overall mechanistic framework that describes transport in different parts of the cell. In the secretory pathway, basic questions, such as how biosynthetic cargo traverses the pathway, are still debated. Historically, emphasis was first put on interpreting function from morphology at the ultrastructural level revealing membrane structures such as the transitional ER, vesicular carriers, vesicular tubular clusters, Golgi cisternae, Golgi stacks and the Golgi ribbon. This emphasis on morphology later switched to biochemistry and yeast genetics yielding many of the key molecular players and their associated functions that we know today. More recently, microscopy studies of living cells incorporating biophysics and system analysis has proven useful and is often used to readdress earlier findings, sometimes with surprising outcomes.
authors.affiliation: Department of Medical Biochemistry, Göteborg University, 413 90 Göteborg, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Kartberg
authors.forename: Markus
authors.initials: M
authors.lastname: Elsner
authors.forename: Linda
authors.initials: L
authors.lastname: Fröderberg
authors.forename: Lennart
authors.initials: L
authors.lastname: Asp
authors.forename: Tommy
authors.initials: T
authors.lastname: Nilsson
mesh_terms: D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D016695:Glycosyltransferases