title: In situ characterization of CD4+ T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance.
abstract: The behavior of antigen-specific CD4+ T lymphocytes during initial exposure to antigen probably influences their decision to become primed or tolerized, but this has not been examined directly in vivo. We have therefore tracked such cells in real time, in situ during the induction of oral priming versus oral tolerance. There were marked contrasts with respect to rate and type of movement and clustering between naive T cells and those exposed to antigen in immunogenic or tolerogenic forms. However, the major difference when comparing tolerized and primed T cells was that the latter formed larger and longer-lived clusters within mucosal and peripheral lymph nodes. This is the first comparison of the behavior of antigen-specific CD4+ T cells in situ in mucosal and systemic lymphoid tissues during the induction of priming versus tolerance in a physiologically relevant model in vivo.
authors.affiliation: Division of Immunology, Infection, and Inflammation, University of Glasgow, Western Infirmary, Glasgow G11 6NT, Scotland, UK.
authors.forename: Bernd H
authors.initials: BH
authors.lastname: Zinselmeyer
authors.forename: John
authors.initials: J
authors.lastname: Dempster
authors.forename: Alison M
authors.initials: AM
authors.lastname: Gurney
authors.forename: David
authors.initials: D
authors.lastname: Wokosin
authors.forename: Mark
authors.initials: M
authors.lastname: Miller
authors.forename: Hsiang
authors.initials: H
authors.lastname: Ho
authors.forename: Owain R
authors.initials: OR
authors.lastname: Millington
authors.forename: Karen M
authors.initials: KM
authors.lastname: Smith
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Rush
authors.forename: Ian
authors.initials: I
authors.lastname: Parker
authors.forename: Michael
authors.initials: M
authors.lastname: Cahalan
authors.forename: James M
authors.initials: JM
authors.lastname: Brewer
authors.forename: Paul
authors.initials: P
authors.lastname: Garside
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D018928:Immunity, Mucosal; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D010047:Ovalbumin
chemical_list: D000941:Antigens; D010047:Ovalbumin