Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century.
Gene targeting in mouse embryonic stem cells has become the 'gold standard' for determining gene function in mammals. Since its inception, this technology has revolutionized the study of mammalian biology and human medicine. Here I provide a personal account of the work that led to the generation of gene targeting which now lies at the centre of functional genomic analysis.
Capecchi
Mario R
MR
Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, 15 North 2030 East, Room 5100, Salt Lake City, Utah 84112-5331, USA. mario.capecchi@genetics.utah.edu
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology; D018390:Gene Targeting / Q000266:history*; D016678:Genome; D023281:Genomics / Q000266:history*; D049673:History, 20th Century; D049674:History, 21st Century; D051379:Mice / Q000235:genetics*; D011995:Recombination, Genetic; D013234:Stem Cells