Simple sensitive and simultaneous high-performance liquid chromatography method of glucoconjugated and non-glucoconjugated porphyrins and chlorins using near infra-red fluorescence detection.
This paper reports, for the first time, a reversed-phase high performance liquid chromatographic method for the simultaneous determination of seven glucoconjugated and non-glucoconjugated porphyrins and chlorins, using near infra-red fluorescence detection. Chromatographic separation was performed on nucleosil-CN analytical column using an isocratic acetonitrile-0.1% (w/v) TFA at pH 1.8 (55:45, v/v) as mobile phase. Wavelength gradient was employed for sensitive detection, porphyrins derivates were monitored at lambda(exc) = 440 nm and lambda(emi) = 680 nm; and chlorins derivates at lambda(exc) = 420 nm, lambda(emi) = 650 nm. The method was validated and applied to monitor the biodegradation of a tri glucoconjugated chlorin derivative, TPC(glu)3, in spiked samples of human serum.
Cañada-Cañada
Florentina
F
Groupe de Chimie Analytique de Paris-Sud, EA 3343, Faculté de Pharmacie, F-92296 Châtenay-Malabry, France. flori@unex.es
Bautista-Sánchez
Antonia
A
Taverna
Myriam
M
Prognon
Patrice
P
Maillard
Philippe
P
Grierson
David S
DS
Kasselouri
Athena
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification*; D006801:Humans; D007259:Infrared Rays; D011166:Porphyrins / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D005960:Glucosides; D011166:Porphyrins; C006969:chlorin