Effect of dendrimers on pure acetylcholinesterase activity and structure.
The effect of polyamidoamine (PAMAM) dendrimers on activity and fluorescence of pure acetylcholinesterase (EC 3.1.1.7.) was studied. It has been shown that all dendrimers studied decreased the enzymatic activity of acetylcholinesterase. This effect depended on the type of dendrimers. The data on the intrinsic fluorescence have shown that the dendrimers changed acetylcholinesterase conformation and the strongest effect was induced by PAMAM G3.5 dendrimer.
Shcharbin
D
D
Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland.
Jokiel
M
M
Klajnert
B
B
Bryszewska
M
M
D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry* / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D050091:Dendrimers; D000110:Acetylcholinesterase