title: Glutamine synthesis is essential for growth of Streptococcus thermophilus in milk and is linked to urea catabolism.
abstract: Growth of a glutamine synthetase-deficient mutant of Streptococcus thermophilus was compared to that of the parent strain in milk that was not supplemented or was supplemented with ammonium chloride, glutamine, or the urease inhibitor flurofamide. It was concluded that one of the functions of urease is to supply ammonia for the synthesis of glutamine.
authors.affiliation: Unité Mixte de Recherche Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, France. monnet@grignon.inra.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Monnet
authors.forename: Diego
authors.initials: D
authors.lastname: Mora
authors.forename: Georges
authors.initials: G
authors.lastname: Corrieu
mesh_terms: D000818:Animals; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000096:biosynthesis*; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA; D048249:Streptococcus thermophilus / Q000235:genetics / Q000254:growth & development*; D014508:Urea / Q000378:metabolism
chemical_list: D005973:Glutamine; D014508:Urea; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase