title: Holistic differential analysis of embryo-induced alterations in the proteome of bovine endometrium in the preattachment period.
abstract: During the peri-implantation period, molecular signaling between embryo and endometrium (layer of tissue lining the uterus lumen) is supposed to be crucial for the maintenance of pregnancy. To investigate embryo-induced alterations in the proteome of bovine endometrium in the preattachment period (day 18), we used monozygotic cattle twins (generated by embryo splitting) as a model eliminating genetic variability as a source for proteome differences. One of the twins was pregnant after the transfer of two in vitro produced blastocysts, while the corresponding twin received a sham-transfer and served as a nonpregnant control. The two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) analysis of the endometrium samples of three twin pairs (pregnant/nonpregnant) revealed four proteins with significantly higher abundance (p < 10(-9)) in each sample derived from the pregnant animals: Rho GDP dissociation inhibitor beta; 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD); soluble NADP(+)-dependent isocitrate dehydrogenase 1; and acyl-CoA-binding protein. To verify the accuracy of the 2-D DIGE quantification, the abundances of 20 alpha-HSD were quantified by a targeted cleavable isotope-coded affinity tag (ICAT) approach. The mass spectrometry-based ICAT quantification matched perfectly the results obtained by 2-D DIGE quantification, demonstrating the accuracy of our data. These results demonstrate that our model (monozygotic twins) in combination with the appropriate analytical tools is particularly suitable for the detection of the proteins involved in the embryo-maternal interactions.
authors.affiliation: Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilian University, Munich, Germany.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Berendt
authors.forename: Thomas
authors.initials: T
authors.lastname: Fröhlich
authors.forename: Susanne E M
authors.initials: SE
authors.lastname: Schmidt
authors.forename: Horst-Dieter
authors.initials: HD
authors.lastname: Reichenbach
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wolf
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Arnold
mesh_terms: D000418:Albumins / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D003545:Cysteine; D015180:Electrophoresis, Gel, Two-Dimensional; D010064:Embryo Implantation / Q000502:physiology; D004717:Endometrium / Q000502:physiology*; D005260:Female; D005456:Fluorescent Dyes; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin; D014430:Twins, Monozygotic
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome; D014357:Trypsin; D003545:Cysteine