An overlay gel method for identification and isolation of bacterial beta-lactamases.
A modification of the iodometric technique using an overlay gel was employed for fast identification and isolation of beta-lactamase types TEM, SHV and AmpC from non-denaturing gels. Osmotic shock preparations of the three beta-lactamases were run on polyacrylamide gels without SDS and ampicillin containing overlay gels were flooded with the iodine solution before being placed on polyacrylamide gel strips. Distinct clear bands appeared in dark blue backgrounds indicating beta-lactamase activity.
Eftekhar
Fereshteh
F
Department of Biology, Faculty of Sciences, Shahid Beheshti University, Evin, Tehran, Iran. f-eftekhar@cc.sbu.ac.ir
Rafiee
Roya
R
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology; D024881:Drug Resistance, Bacterial / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007455:Iodine / Q000378:metabolism; D007769:Lactams / Q000378:metabolism* / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods*; D013213:Starch / Q000378:metabolism; D001618:beta-Lactamases / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D007769:Lactams; D013213:Starch; D007455:Iodine; D001618:beta-Lactamases