Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell.
Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1alpha, IL-1beta, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
Kim
Dong Hyun
DH
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Yoo
Keon Hee
KH
Choi
Kyung Suk
KS
Choi
Jaewon
J
Choi
Sang-Yun
SY
Yang
Sung-Eun
SE
Yang
Yoon-Sun
YS
Im
Ho Joon
HJ
Kim
Kye Hyun
KH
Jung
Hye Lim
HL
Sung
Ki Woong
KW
Koo
Hong Hoe
HH
D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers; D020869:Gene Expression Profiling; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D016207:Cytokines; D017931:DNA Primers; D006133:Growth Substances