Targeting antigens to murine and human M-cells with Aleuria aurantia lectin-functionalized microparticles.
Neuraminidases act as a virulence factors for several pathogens that invade the human body through Peyer's patch M-cells. Because of the structural similarity of Aleuria aurantia lectin (AAL) to neuraminidases, we hypothesized that AAL might also target human M-cells. In an in vitro human M-cell co-culture model significantly more particles were transported across the epithelium when microparticles were functionalized with AAL versus those that were not. Moreover, high concentrations of AAL induced no detectable cytotoxic effects on the related intestinal epithelial cell cultures, epithelial Caco2- and HT29-MTX-E12-cells. Upon incubation with AAL, PBMCs of allergic volunteers proliferated in response to AAL and secreted the cytokines, IL-2, IFN-gamma, IL-10 and IL-5 in a concentration-dependent manner, indicating immune-stimulatory properties of the lectin. We conclude that AAL-coated microparticles may have the potential to target entrapped antigens to human M-cells for oral vaccination.
Roth-Walter
Franziska
F
Center of Physiology and Pathophysiology, Medical University of Vienna, AKH-3Q, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
Bohle
Barbara
B
Schöll
Isabella
I
Untersmayr
Eva
E
Scheiner
Otto
O
Boltz-Nitulescu
George
G
Gabor
Franz
F
Brayden
David J
DJ
Jensen-Jarolim
Erika
E
D000485:Allergens / Q000276:immunology; D018938:Caco-2 Cells; D018920:Coculture Techniques; D004306:Dose-Response Relationship, Immunologic; D016503:Drug Delivery Systems; D019073:HT29 Cells; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D037102:Lectins / Q000276:immunology; D008863:Microspheres; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage*
D000485:Allergens; D007376:Interleukin-2; D037102:Lectins; D014612:Vaccines; C064549:lectin, Aleuria aurantia; D007371:Interferon-gamma