cDNA AFLP-based techniques for studying transcript profiles in horses.
The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression in species, such as the equine one, for which little genomic information is available. In the present study, we evaluated two techniques for studying mRNA profiles in horse tissue, one referred to the cDNA-amplified fragment length polymorphism (AFLP) that we called C-AFLP (classical cDNA-AFLP) protocol and the other to ordered differential display (ODD) with some modifications that we named S-AFLP (systematic cDNA-AFLP). Both techniques can be applied in live animals because of the small amount of sample required. We applied the S-AFLP to investigate horse transcript profile modifications during physical exercise. We found two transcripts that are mostly expressed during exercise and immediately after the end of it.
Cappelli
K
K
Centro di Studio del Cavallo Sportivo, Facoltà di Medicina Veterinaria, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. vete7@unipg.it
Porceddu
A
A
Verini-Supplizi
A
A
Capomaccio
S
S
Marchis
F De
FD
Falcinelli
M
M
Gaiti
A
A
Silvestrelli
M
M
D000818:Animals; D018076:DNA, Complementary; D020869:Gene Expression Profiling / Q000662:veterinary*; D006736:Horses / Q000235:genetics*; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000032:analysis
D018076:DNA, Complementary; D012333:RNA, Messenger