The transcriptional activator HAP4 is a high copy suppressor of an oxa1 yeast mutation.
Oxa1p is a key component of the machinery for the insertion of membrane proteins in mitochondria, and in the yeast Saccharomyces cerevisiae, the deletion of OXA1 impairs the biogenesis of the three respiratory complexes of dual genetic origin. Oxa1p is formed from three domains located in the intermembrane space, the inner membrane and the mitochondrial matrix. We have isolated a high copy suppressor able to partially compensate for the respiratory deficiency caused by a large deletion of the matrix domain. We show that the suppressor gene corresponds to the nuclear transcriptional activator Hap4p which is known to regulate respiratory functions.
Hlavacek
Otakar
O
Centre de Génétique Moléculaire, Avenue de la Terrasse, 91198-Gif sur Yvette, France.
Bourens
Myriam
M
Salone
Veronique
V
Lachacinski
Nicole
N
Lemaire
Claire
C
Dujardin
Geneviève
G
D015153:Blotting, Western; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D023081:CCAAT-Binding Factor; C061437:HAP4 protein, S cerevisiae; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C090858:OXA1 protein; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003576:Electron Transport Complex IV