[The role of circadian gene period1 in morphine reward in mice].
OBJECTIVE
To investigate the role of circadian gene Period1 on drug dependence.


METHODS
The ribozyme cleave the Period1 mRNA (per1RZ) was designed and the per1RZ expression vector (pcDNA 3.1-per1RZ) was constructed based on eukaryotic expression vector pcDNA 3.1. In vitro cleavage experiment proved the target cleave Period1 mRNA ability of per1RZ. Conditional place preference (CPP) paradigm used to investigate the effect of intracerebroventricular (ICV) injection of pcDNA 3.1-per1RZ on drug reward in BALB/C mice.


RESULTS
Quantitative analysis of in vitro cleavage experiment showed the efficacy of ribozyme per1RZ, about 60% of the Period1 mRNA was cleaved by ribozyme per1RZ. CPP test displayed the block of drug reward in pcDNA 3.1-per1RZ ICV injection group. Period1 expression in brain was attenuated of pcDNA 3.1-per1RZ ICV injection group demonstrated by western blot.


CONCLUSIONS
Interfere the Period1 expression in brain could attenuate the psychological dependence of drug in mammals.
Wang
Yue-qi
YQ
School of Basic Medicine and Forensic, Sichuan University, Chengdu, China.
Zhou
Wei
W
Liu
Yan-you
YY
Liu
Ying-hui
YH
Peng
Tao
T
Wang
Zheng-rong
ZR
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000235:genetics*; D004195:Disease Models, Animal; D015316:Genetic Therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000494:pharmacology*; D009021:Morphine Dependence / Q000188:drug therapy* / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000494:pharmacology*; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000378:metabolism; D019966:Substance-Related Disorders / Q000188:drug therapy* / Q000235:genetics
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D009020:Morphine