title: Permselectivity and pH-dependence of Plasmodium falciparum-induced anion currents in human erythrocytes.
abstract: Intraerythrocytic survival of the malaria pathogen Plasmodium falciparum requires delivery of nutrients and disposal of waste products across the host erythrocyte membrane. Recent patch-clamp experiments have demonstrated inwardly and outwardly rectifying anion conductances in infected but not in control erythrocytes. A ClC-2-generated fraction of the inwardly rectifying current is activated by cell swelling and presumably subserves host cell volume regulation. In contrast, the outwardly rectifying current is insensitive to cell volume but allows the passage of lactate and is involved in the transport of nutrients. The present study was performed to characterize the permselectivity and pH sensitivity of the anion conductances using whole-cell recording. The outwardly rectifying and the inwardly rectifying currents exhibited permselectivities of Cl- > or = Br- approximately I- > SCN- and SCN- > I- > Br- > Cl-, respectively, as evident from the reversal potentials recorded under biionic conditions. While the inwardly rectifying current was not affected significantly by alterations of pH between 6.0 and 8.4, the outward rectifier was inhibited strongly by alkalinization to pH > or = 7.8. Fluxes of 14C-lactate and parasite growth were decreased markedly by the increase of bath pH, an effect that may at least in part be due to inhibition of the outward rectifier and subsequently impaired transport across the erythrocyte membrane.
authors.affiliation: Department of Physiology I, Eberhard-Karls-University Tübingen, Gmelinstrasse 5, 72076 Tubingen. christophe.duranton@uni-tuebingen.de
authors.forename: Christophe
authors.initials: C
authors.lastname: Duranton
authors.forename: Valerie
authors.initials: V
authors.lastname: Tanneur
authors.forename: Verena
authors.initials: V
authors.lastname: Brand
authors.forename: Ciprian D
authors.initials: CD
authors.lastname: Sandu
authors.forename: Canan
authors.initials: C
authors.lastname: Akkaya
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Huber
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism*; D018118:Chloride Channels / Q000502:physiology*; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010539:Permeability; D010963:Plasmodium falciparum / Q000502:physiology*
chemical_list: D000838:Anions; D018118:Chloride Channels; D019344:Lactic Acid