Primary afferent neurons intrinsic to the guinea-pig intestine, like primary afferent neurons of spinal and cranial sensory ganglia, bind the lectin, IB4.
The plant lectin, IB4, binds to the surfaces of primary afferent neurons of the dorsal root and trigeminal ganglia and is documented to be selective for nociceptive neurons. Physiological data suggest that the intrinsic primary afferent neurons within the intestine are also nociceptors. In this study, we have compared IB4 binding to each of these neuron types in the guinea-pig. The only neurons in the intestine to be readily revealed by IB4 binding have Dogiel-type-II morphology; these neurons have been previously identified as intrinsic primary afferent neurons. Most of the neurons that are IB4-positive in the myenteric plexus are calbindin-immunoreactive, whereas those in the submucosal ganglia are immunoreactive for NeuN. The neurons that bind IB4 strongly have a similar appearance in enteric, dorsal root and trigeminal ganglia. Binding is to the cell surface, to the first part of axons and to cytoplasmic organelles. A low level of binding was found in the extracellular matrix. A few other neurons in all ganglia exhibit faint staining with IB4. Strongly reactive neurons are absent from the gastric corpus. Thus, IB4 binding reveals primary afferent neurons with similar morphologies, patterns of binding and physiological roles in enteric, dorsal root and trigeminal ganglia.
Hind
Anderson
A
Department of Anatomy & Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
Migliori
Massimo
M
Thacker
Michelle
M
Staikopoulos
Vasiliki
V
Nurgali
Kulmira
K
Chiocchetti
Roberto
R
Furness
John B
JB
D000818:Animals; D001369:Axons / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D064026:Calbindins; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape / Q000502:physiology; D004268:DNA-Binding Proteins; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000379:methods; D007422:Intestines / Q000294:innervation* / Q000502:physiology; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013368:Submucous Plexus / Q000166:cytology / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism; D017833:Visceral Afferents / Q000166:cytology / Q000378:metabolism
D064026:Calbindins; D004268:DNA-Binding Proteins; C045475:Griffonia simplicifolia lectins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D037121:Plant Lectins; D064030:S100 Calcium Binding Protein G