Co-expressed recombinant human Translin-Trax complex binds DNA.
Trax, expressed alone aggregates into insoluble complexes, whereas upon co-expression with Translin becomes readily soluble and forms a stable heteromeric complex ( approximately 430 kDa) containing both proteins at nearly equimolar ratio. Based on the subunit molecular weights, estimated by MALDI-TOF-MS, the purified complex appears to comprise of either an octameric Translin plus a hexameric Trax (calculated MW 420 kDa) or a heptamer each of Trax and Translin (calculated MW 425 kDa) or a hexameric Translin plus an octameric Trax (calculated MW 431 kDa). The complex binds single-stranded/double-stranded DNA. ssDNA gel-shifted complex shows both proteins at nearly equimolar ratio, suggesting that Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent.
Gupta
Gagan D
GD
Synchrotron Radiation Section, Bhabha Atomic Research Centre, Mumbai 400 085, India.
Makde
Ravindra D
RD
Kamdar
Radhika P
RP
D'Souza
Jacinta S
JS
Kulkarni
Meeta G
MG
Kumar
Vinay
V
Rao
Basuthkar J
BJ
D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D011994:Recombinant Proteins; C095563:TSN protein, human; C498305:TSNAX protein, human; D004247:DNA