Determination of sinomenine in human plasma by HPLC/ESI/ion trap mass spectrum.
BACKGROUND
Sinomenine is a pure alkaloid with a variety of pharmacological actions including anti-inflammation, immuno-suppression, analgesia, antihypertension, and antiarrhythmia. Methods have been developed to measure sinomenine in rats and rabbits through high-performance liquid chromatography (HPLC). But only one report described a method for determination of sinomenine in humans.


METHODS
We developed a method to quantitate sinomenine in human plasma based on liquid chromatography-ion trap mass spectrometry coupled with electrospray ionization (HPLC/ESI/ion trap mass spectrum).


RESULTS
The calibration curve (r2=0.9993) of sinomenine was established with standard solutions. The mean inter-day and intra-day CVs<15%. The limit of detection for sinomenine was 0.5 ng/ml. The relative recovery of high, middle and low concentration was 99%, 103%, and 118%, respectively.


CONCLUSIONS
The method is simple, rapid, sensitive, specific, and accurate enough for determination of sinomenine in human plasma and may be used to investigate the metabolism and pharmacokinetics of sinomenine.
Yao
Yong-Mei
YM
Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China.
Tan
Zhi-Rong
ZR
Hu
Zhe-Yi
ZY
Guo
Xin
X
Cheng
Ze-Neng
ZN
Wang
Lian-Sheng
LS
Zhou
Hong-Hao
HH
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009019:Morphinans / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization
D009019:Morphinans; C009271:sinomenine