title: Green light emitting diode irradiation enhances fibroblast growth impaired by high glucose level.
abstract: OBJECTIVE
  The chronic metabolic disorder diabetes mellitus is an important cause of morbidity and mortality due to a series of common secondary metabolic complications, such as the development of severe, often slow healing skin lesions. In view of promoting the wound-healing process in diabetic patients, this preliminary in vitro study investigated the efficacy of green light emitting diode (LED) irradiation on fibroblast proliferation and viability under hyperglycemic circumstances.
  
  
  METHODS
  To achieve hyperglycemic circumstances, embryonic chicken fibroblasts were cultured in Hanks' culture medium supplemented with 30 g/L glucose. LED irradiation was performed on 3 consecutive days with a probe emitting green light (570 nm) and a power output of 10 mW. Each treatment lasted 3 min, resulting in a radiation exposure of 0.1 J/cm2.
  
  
  RESULTS
  A Mann-Whitney U test revealed a higher proliferation rate (p = 0.001) in all irradiated cultures in comparison with the controls.
  
  
  CONCLUSIONS
  According to these results, the effectiveness of green LED irradiation on fibroblasts in hyperglycemic circumstances is established. Future in vivo investigation would be worthwhile to investigate whether there are equivalent positive results in diabetic patients.
authors.affiliation: Department of Rehabilitation Sciences and Physiotherapy, Ghent University, 9000 Ghent, Belgium. elke.vinck@UGent.be
authors.forename: Elke M
authors.initials: EM
authors.lastname: Vinck
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Cagnie
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Cornelissen
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Declercq
authors.forename: Dirk C
authors.initials: DC
authors.lastname: Cambier
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects*; D005947:Glucose / Q000494:pharmacology; D028022:Low-Level Light Therapy; D013549:Sweetening Agents / Q000494:pharmacology
chemical_list: D013549:Sweetening Agents; D005947:Glucose