title: In vitro generation of mature neutrophils from canine Lin- bone marrow cells.
abstract: The major goal of this work was to describe the in vitro generation of mature functional neutrophils derived from a canine enriched haematopoietic progenitor cell population. We have utilised lineage depletion by immunomagnetic selection to isolate a canine haematopoietic progenitor cell population. The physical, immunological, metabolical and morphological methodologies employed in this study have permitted us to isolate and define a cell population enriched in Rh-123low and CD34+ cells. Irradiated pre-established long-term bone marrow cultures (LTBMC) were utilised to determine the self-renewal ability of lineage negative (Lin-) cells, as well as their capacity to differentiate into mature functional neutrophils. The authors demonstrate for the first time that canine neutrophils derived from Lin- cells are able to produce oxyradicals, express a specific neutrophil surface antigen, and contain gelatinase granules. These characteristics enable them to migrate through basement membranes to act as a first line defence mechanism. The fact that these cells are able to differentiate into functional mature cells, and give rise to long-term culture-initiating cells (LTC-IC) after 35 days of culture, allows the authors to assure that the isolated canine enriched haematopoietic cell population exhibit functional characteristics, associated with primitive haematopoietic cells.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Leticia G
authors.initials: LG
authors.lastname: León
authors.forename: Luciana K
authors.initials: LK
authors.lastname: Ostronoff
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Fermín
authors.forename: Cristina
authors.initials: C
authors.lastname: Fragío
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Hans-Jochem
authors.initials: HJ
authors.lastname: Kolb
authors.forename: Concepción
authors.initials: C
authors.lastname: Tejero
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004285:Dogs / Q000033:anatomy & histology / Q000276:immunology* / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018189:Immunomagnetic Separation; D066298:In Vitro Techniques; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D016897:Respiratory Burst
chemical_list: D018093:Gelatinases