Mu opioid receptor-containing neurons mediate electroacupuncture-produced anti-hyperalgesia in rats with hind paw inflammation.
Previous studies showed that electroacupuncture (EA) significantly attenuates inflammatory hyperalgesia in a complete Freund's adjuvant (CFA)-induced inflammatory pain rat model. The present study demonstrates that pretreatment with Derm-sap, a selective toxin for neurons that contain mu opioid receptor (MOR), specifically decreases MOR and blocks EA anti-hyperalgesia. These data suggest that spinal MOR-containing neurons are involved in the processes by which EA produces anti-hyperalgesia.
Zhang
Rui-Xin
RX
Center for Integrative Medicine, School of Medicine, University of Maryland, 3rd Floor, James Kernan Hospital Mansion, 2200 Kernan Drive, Baltimore, MD 21207, USA.
Wang
Linbo
L
Liu
Bing
B
Qiao
Jian-Tian
JT
Ren
Ke
K
Berman
Brian M
BM
Lao
Lixing
L
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D015671:Electroacupuncture / Q000379:methods*; D005620:Freund's Adjuvant / Q000009:adverse effects; D006614:Hindlimb / Q000187:drug effects / Q000294:innervation*; D006930:Hyperalgesia / Q000209:etiology / Q000628:therapy*; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation / Q000139:chemically induced / Q000150:complications; D037102:Lectins / Q000378:metabolism; D008297:Male; D009699:N-Glycosyl Hydrolases; D009474:Neurons / Q000378:metabolism*; D018847:Opioid Peptides; D010147:Pain Measurement / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D017465:Receptors, Opioid, delta / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D012867:Skin / Q000187:drug effects / Q000294:innervation; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
D037102:Lectins; D018847:Opioid Peptides; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D011993:Recombinant Fusion Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C431163:dermorphin-saporin; D013373:Substance P; D005620:Freund's Adjuvant; D009699:N-Glycosyl Hydrolases; D000076985:Saporins