The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model.
The quantum yield of the primary photoreaction of HR478 was determined as phi(green) = 0.34 +/- 0.02 and that of the photochemical reconversion of HRL410 into HR578 as phi(blue) = 0.01 +/- 0.004. From steady-state illumination and flash-photolysis experiments, a quantitative description of HRL410 formation and decay was made leading to a model of the photocycle in isolated, light-adapted HR. This model satisfies all known facts about HR including its photochronic behaviour.
Oesterhelt
D
D
Max-Planck-Institut für Biochemie, D-8033, Martinsried, FRG.
Hegemann
P
P
Tittor
J
J
D018118:Chloride Channels / Q000378:metabolism*; D019605:Euryarchaeota / Q000378:metabolism; D025602:Halorhodopsins / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008956:Models, Chemical; D010782:Photolysis
D018118:Chloride Channels; D025602:Halorhodopsins