Purification of glucose oxidase from Aspergillus niger by liquid-liquid cationic reversed micelles extraction.
The aim of this work was to select the operating conditions for the extraction and recovery of glucose oxidase (GOX) by reversed micelles from mixtures of commercial enzyme and Aspergillus niger homogenates. For this purpose, the influence of the main operating parameters (pH, surfactant concentration, and presence of cell debris or not) on GOX extraction was investigated at 25 degrees C. Without cell debris, the highest yield of GOX activity recovery (90.8%) was obtained performing (a) the forward extraction in isooctane as solvent and hexanol and butanol as cosolvents at 76/6/18 ratio, pH 7.0, 0.2 M cetyl trimethylammonium bromide as cationic surfactant, and electric conductivity of 5.0 mS cm(-1) and (b) the backward extraction at pH 5.5. Forward and backward extractions furnished comparable results when using raw homogenate, which demonstrates a negligible impact of the presence of cell debris on the process. The highest extraction yield (94%) was obtained under the same forward and backward conditions adopted without cell debris. The promising results of this work suggest that the proposed methodology could be profitably exploited at an industrial level.
Ferreira
Luis Fernando Peffi
LF
Biotechnology Interunit Graduation Program IPT/Butantã/USP, P.O. Box 66083, São Paulo SP, Brazil.
Taqueda
Maria Elena
ME
Converti
Attilio
A
Vitolo
Michele
M
Pessoa
Adalberto
A
D001234:Aspergillus niger / Q000201:enzymology*; D002498:Centrifugation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D005949:Glucose Oxidase / Q000096:biosynthesis* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010865:Pilot Projects; D012996:Solutions; D012997:Solvents / Q000737:chemistry*
D008823:Micelles; D012996:Solutions; D012997:Solvents; D005949:Glucose Oxidase