Adsorptive stripping voltammetric technique for the rapid determination of tobramycin on the hanging mercury electrode.
A linear sweep adsorptive stripping voltammetric method (AdS-LSV) for the determination of tobramycin (TOB) has been proposed for the first time. The method is based on the formation of the voltammetrically active iso-butyraldehyde derivative of TOB and the electrochemical behavior of TOB iso-butyraldehyde derivative has been studied. TOB iso-butyraldehyde derivative exhibits a sensitive cathodic peak at -1.40 V (versus SCE) in a medium of B-R buffer (pH 9.8) with a scan rate of 90 mVs(-1) after a preconcentration period of 120 s at -1.10 V (versus SCE). The linear concentration range of application was 6.87 x 10(-9) - 3.44 x 10(-7) mol L(-1) of TOB, with a relative standard deviation of 4.4% (for a level of 1.0 x 10(-7) mol L(-1)) and a detection limit of 3.44 x 10(-9) mol L(-1). The method was applied to the direct determination of TOB in injectable formulations and spiked urine and serum samples.
Sun
Nan
N
College of Chemical Engineering and Material Science, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China. sunnan@zjut.edu.cn
Mo
Wei-Min
WM
Shen
Zhen-Lu
ZL
Hu
Bao-Xiang
BX
D000327:Adsorption; D000447:Aldehydes / Q000737:chemistry; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006801:Humans; D008628:Mercury / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014031:Tobramycin / Q000032:analysis* / Q000097:blood / Q000652:urine
D000447:Aldehydes; D008628:Mercury; C018475:butyraldehyde; D014031:Tobramycin