title: Francisella tularensis live vaccine strain: proteomic analysis of membrane proteins enriched fraction.
abstract: Proteome analysis of Gram-negative facultative intracellular pathogen Francisella tularensis (F. tularensis) live vaccine strain has been performed only on whole-cell extracts so far. This is the first study dealing with the analysis of the membrane subproteome of this microorganism. A fraction enriched in membrane proteins obtained by carbonate extraction was separated using two-dimensional electrophoresis and all visualized spots were identified by mass spectrometry. The reference map is the basis for further comparative analyses of virulent and non-virulent F. tularensis strains.
authors.affiliation: Proteome Center for the Study of Intracellular Parasitism of Bacteria, Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic. klimtova@pmfhk.cz
authors.forename: Ivona
authors.initials: I
authors.lastname: Pávková
authors.forename: Martin
authors.initials: M
authors.lastname: Hubálek
authors.forename: Jana
authors.initials: J
authors.lastname: Zechovská
authors.forename: Juraj
authors.initials: J
authors.lastname: Lenco
authors.forename: Jirí
authors.initials: J
authors.lastname: Stulík
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005604:Francisella tularensis / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014613:Vaccines, Attenuated / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D014613:Vaccines, Attenuated