title: A BAC-based physical map of the Drosophila buzzatii genome.
abstract: Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps, and provide useful resources for sequencing entire genomes. Drosophila buzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation, and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and an approximately 18x expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be approximately 23x. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9555 clones, and assembled them using FingerPrint Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670 singletons. Finally, we anchored 181 large contigs (containing 7788 clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
authors.forename: Josefa
authors.initials: J
authors.lastname: González
authors.forename: Michael
authors.initials: M
authors.lastname: Nefedov
authors.forename: Ian
authors.initials: I
authors.lastname: Bosdet
authors.forename: Ferran
authors.initials: F
authors.lastname: Casals
authors.forename: Oriol
authors.initials: O
authors.lastname: Calvete
authors.forename: Alejandra
authors.initials: A
authors.lastname: Delprat
authors.forename: Heesun
authors.initials: H
authors.lastname: Shin
authors.forename: Readman
authors.initials: R
authors.lastname: Chiu
authors.forename: Carrie
authors.initials: C
authors.lastname: Mathewson
authors.forename: Natasja
authors.initials: N
authors.lastname: Wye
authors.forename: Roger A
authors.initials: RA
authors.lastname: Hoskins
authors.forename: Jacqueline E
authors.initials: JE
authors.lastname: Schein
authors.forename: Pieter
authors.initials: P
authors.lastname: de Jong
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ruiz
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D004330:Drosophila / Q000235:genetics*; D017344:Genes, Insect; D015698:Genomic Library; D017422:Sequence Analysis, DNA