title: Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle.
abstract: Klp67A is a member of the Kip3 subfamily of microtubule destabilising kinesins, the loss of which results in abnormally long and stable pre-anaphase microtubules. Here we examine its role during cytokinesis in Drosophila primary spermatocytes that require the coordinated interaction of an interior and peripheral set of central spindle microtubules. In mutants anaphase B spindles elongated with normal kinetics but bent towards the cortex. Both peripheral and interior spindle microtubules then formed diminished bundles of abnormally positioned central spindle microtubules associated with the pavarotti-KLP and KLP3A motor proteins. The minus ends of these were poorly aligned as revealed by Asp protein localisation. Furrows always initiated at the sites of central spindle bundles but could be unilateral or nonequatorially positioned. Ectopic furrows were stimulated by the interior central spindle and formed only after this structure buckled and contacted the cortex. Furrows often halted and regressed as they could not be sustained by the central spindles that became increasing unstable over time and often completely degraded. Consistent with this, actin and anillin failed to form homogenous bands. Thus, the Klp67A microtubule catastrophe factor is required for cytokinesis by regulating both the formation and stability of the central spindle.
authors.affiliation: Department of Genetics, University of Cambridge, UK.
authors.forename: Melanie K
authors.initials: MK
authors.lastname: Gatt
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Savoian
authors.forename: Maria G
authors.initials: MG
authors.lastname: Riparbelli
authors.forename: Chiara
authors.initials: C
authors.lastname: Massarelli
authors.forename: Giuliano
authors.initials: G
authors.lastname: Callaini
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
mesh_terms: D000199:Actins / Q000378:metabolism; D000705:Anaphase; D000818:Animals; D018385:Centrosome / Q000378:metabolism; D003285:Contractile Proteins / Q000378:metabolism; D048749:Cytokinesis; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D000199:Actins; D003285:Contractile Proteins; D029721:Drosophila Proteins; C104939:KLP67A protein, Drosophila; D008869:Microtubule-Associated Proteins; C095886:anillin