Surface display of recombinant proteins on Bacillus thuringiensis spores.
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.
Du
Cheng
C
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA. cdu@unmc.edu
Chan
Wing C
WC
McKeithan
Timothy W
TW
Nickerson
Kenneth W
KW
D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D018274:Electroporation; D004731:Endotoxins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006460:Hemolysin Proteins; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D010080:Oxazoles / Q000276:immunology*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D057127:Single-Chain Antibodies; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial
C119678:4-ethoxymethylene-2-phenyl-2-oxazoline-5-one; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007135:Immunoglobulin Variable Region; D008164:Luminescent Proteins; D010080:Oxazoles; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C410726:scFV green fluorescent protein, recombinant; D049452:Green Fluorescent Proteins