Investigation of acute toxicity of chlorpyrifos-methyl on guppy Poecilia reticulata.
Static bioassays were made to determine acute toxicity of chlorpyrifos-methyl, a wide spectrum organophosphorus insecticide and potential toxic pollutant of aquatic ecosystem, Guppy fish (Poecilia reticulata). Bioassays were made at a regulated temperature of 22+/-1 degrees C and were repeated three times. Lethal doses of the insecticides were determined using LC50 software programme of U.S. EPA based on Finney's Probit Analysis statistical method. The 96 h LC50 value and 95% confidence limit of chlorpyrifos-methyl for Guppy was estimated as 1.79 (1.47-2.10) mg/l. The fish exposed to chlorpyrifos-methyl exhibited behavioral changes in the form of neurotoxin toxicity: less general activity than control group, loss of equilibrium, erratic swimming and staying motionless at a certain location generally at mid-water level for prolonged periods. The 1 mg/l (lowest) concentration had similar behavior (NOEC) with the control group.
Selvi
Mahmut
M
Department of Biology Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey.
Sarikaya
Rabia
R
Erkoç
Figen
F
Koçak
Oner
O
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001681:Biological Assay; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D011036:Poecilia / Q000502:physiology*; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007306:Insecticides; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl