title: Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.
abstract: Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.
authors.affiliation: Immune System Section, Department of Immunology, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan 305-0856. kikuma@affrc.go.jp
authors.forename: Reiko
authors.initials: R
authors.lastname: Nagata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muneta
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D002417:Cattle; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000096:biosynthesis*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D011994:Recombinant Proteins; D007371:Interferon-gamma