Removing peroxide impurities from ether improves the quality of globin chains for biosynthetic studies.
The diagnosis of the different forms of thalassemia is one of the important applications of analysis of globin chains. These analyses are done by high performance liquid chromatography (HPLC) using a MONO-S cation exchange column and ether is used for washing the globin powder in the final step. The presence of peroxide impurities in ether could change the structure of the globin chains. In the chromatograms, these modified globins appear as separated peaks next to the unmodified globin peaks. In these cases, the alpha/beta ratio exceed by artifact the correct value. Our study demonstrates that diagnostic centers should ensure that the ether they use is pure.
Mirzazadeh
Roghieh
R
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran. rmirzazadeh@yahoo.com
Khatami
Shohreh
S
Bayat
Parastoo
P
Zamani
Zahra
Z
Sadeghi
Sedigheh
S
Roohi
Soghra
S
Saidi
Parinaz
P
D016477:Artifacts; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004987:Ethers / Q000737:chemistry*; D005914:Globins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D010545:Peroxides / Q000737:chemistry*; D012016:Reference Values; D012680:Sensitivity and Specificity
D004987:Ethers; D010545:Peroxides; D005914:Globins