Expression of 14-3-3 zeta and interaction with protein kinase C in the rat retina in early diabetes.
OBJECTIVE
The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes.


METHODS
Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabetic rats after 6 weeks of diabetes. PKC activity was examined using a PKC assay.


RESULTS
In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased.


CONCLUSIONS
These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.
Kim
Y H
YH
Department of Anatomy and Neurobiology, College of Medicine, Institute of Health Science, Gyeongsang National University, Jinju, South Korea.
Kim
Y S
YS
Kang
S S
SS
Noh
H S
HS
Kim
H J
HJ
Cho
G J
GJ
Choi
W S
WS
D048948:14-3-3 Proteins / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000503:physiopathology*; D005786:Gene Expression Regulation; D008297:Male; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000503:physiopathology*; D014158:Transcription, Genetic
D048948:14-3-3 Proteins; D012333:RNA, Messenger; D011493:Protein Kinase C