PTEN and Egr-1 expression in thyroid proliferative lesions.
PTEN is a tumor suppressor gene that inhibits cell cycle progression. Recent data support that PTEN transcription is upregulated by Egr-1. The present study evaluated the immunohistochemical expression of PTEN and Egr-1 in normal thyroid and in its benign and malignant proliferative lesions. PTEN expression was cytoplasmic. The median percentage of normal cells with positive staining was 97.5%. It was similar in nodular hyperplasia, adenoma and papillary carcinoma. Follicular and undifferentiated carcinoma presented a significant decrease in the percentage of positive cells (P=0.027 and P=0.004). Egr-1 expression was nuclear. The median percentage of positivity was similar in normal tissue (29.75%), nodular hyperplasia (30.5%) and papillary carcinoma (28.25%). Adenomas, follicular carcinomas and undifferentiated carcinomas showed a significant decrease of nuclear positivity (P=0.001; P=0.001 and P=0.004, respectively).
Di Loreto
Carla
C
Anatomic Pathology, University of Udine, Udine, Italy.
Tell
Gianluca
G
Pestrin
Marta
M
Pandolfi
Maura
M
Damante
Giuseppe
G
Puglisi
Fabio
F
D000236:Adenoma / Q000235:genetics* / Q000503:physiopathology*; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000503:physiopathology*; D016022:Case-Control Studies; D002453:Cell Cycle; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D051766:Early Growth Response Protein 1; D020869:Gene Expression Profiling; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics*; D007150:Immunohistochemistry; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis* / Q000235:genetics*; D013959:Thyroid Diseases / Q000235:genetics* / Q000503:physiopathology*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000503:physiopathology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human