Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes.
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were exhibited on immunoblot profiles against bovine IgM, IgE, IgA, and IgG, respectively. A 46 kDa band was probed as a common antigenic band except IgA; 69 kDa band was bovine IgM and IgE; 33, 37, 55, and 79 kDa bands were bovine IgM and IgG; 72 kDa band was found IgM and IgA profiles. Based on the analysis, it appeared that different immunoglobulin classes recognizing different antigenic molecules were cooperating to cope with neosporosis.
Shin
Yong-Seung
YS
Institue of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea.
Lee
Eung-Goo
EG
Jung
Tae-Sung
TS
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D003048:Coccidiosis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D015151:Immunoblotting; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D018688:Neospora / Q000276:immunology*
D000953:Antigens, Protozoan; D007130:Immunoglobulin Idiotypes