Preparation and characterization of intelligent core-shell nanoparticles based on poly(D,L-lactide)-g-poly(N-isopropyl acrylamide-co-methacrylic acid).
New thermo-responsive, pH-responsive, and biodegradable nanoparticles comprised of poly(D,L-lactide)-graft-poly(N-isopropyl acrylamide-co-methacrylic acid) (PLA-g-P(NIPAm-co-MAA)) were developed by grafting biodegradable poly(D,L-lactide) onto N-isopropyl acrylamide and methacrylic acid. A core-shell type nano-structure was formed with a hydrophilic outer shell and a hydrophobic inner core, which exhibited a phase transition temperature above 37 degrees C suitable for biomedical application. Upon heating above the phase transition temperature, PLA-g-P(NIPAm-co-MAA) nanoparticle showed a polarity increase of pyrene in either buffer solution or intra-hepato-carcinoma cells as determined by fluorescence measurement, indicating that the structure of nanoparticles caused leakages from outer shell copolymers aggregation and collapsed. The drug loading level of 5-fluorouracil (5-FU) encapsulated in the PLA-g-P(NIPAm-co-MAA) nanoparticles can be as high as 20%. The release of 5-FU from nanoparticles was strongly controlled by the pH in the aqueous solution. Based on these results, PLA-g-P(NIPAm-co-MAA) nanoparticles can be used as a drug carrier for intracellular delivery of anti-cancer drug.
Lo
Chun-Liang
CL
Department of Chemical Engineering, National Tsing Hua University, 101 Section, 2 Kuang Fu Road, Hsinchu, 300 Taiwan, ROC.
Lin
Ko-Min
KM
Hsiue
Ging-Ho
GH
D000178:Acrylamides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D005472:Fluorouracil / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D049329:Nanostructures; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics; D012995:Solubility; D013696:Temperature
D000178:Acrylamides; D004337:Drug Carriers; D011091:Polyesters; D011109:Polymethacrylic Acids; C503833:poly(D,L-lactide)-g-poly(N-isopropyl acrylamide-co-methacrylic acid); C085085:poly(N-isopropylacrylamide-co-methacrylic acid); C033616:poly(lactide); D005472:Fluorouracil