Intermittent hypoxia modulates Na+ channel expression in developing mouse brain.
Because our previous work showed that intermittent hypoxia alters neuronal excitability and Na+ current density, we examined in this work the effect of intermittent hypoxia on Na+ channel subtypes using 3H-saxitoxin (3H-STX) autoradiography and immunoblotting. Mice were exposed to intermittent hypoxia for 2 or 4 weeks from postnatal day 2 or 3. A 2-week intermittent hypoxia reduced cerebral STX binding density with significant decrease in Na(v)1.2 in the rostral and Na(v)1.1 in the caudal regions. In contrast, a 4-week intermittent hypoxia tended to increase STX binding density in most brain regions. Our data suggest that intermittent hypoxia differentially regulates plasma membrane Na+ channels in the developing brain, depending on duration of intermittent hypoxia.
Zhao
Peng
P
Department of Pediatrics, Yale University School of Medicine, 333 Cedar Street, LMP 3107, New Haven, CT 06520, USA.
Xue
Jin
J
Gu
Xiang-Qun
XQ
Haddad
Gabriel G
GG
Xia
Ying
Y
D000222:Adaptation, Physiological; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D002534:Hypoxia, Brain / Q000378:metabolism*; D051379:Mice; D015222:Sodium Channels / Q000378:metabolism*; D014018:Tissue Distribution
D015222:Sodium Channels