Neuronal markers expression of NGF-primed bone marrow cells (BMCs) transplanted in the brain of 6-hydroxydopamine and ibotenic acid lesioned littermate mice.
In the present study, we aim to show that non-adherent bone marrow cells (BMCs) express TrkA, the nerve growth factor (NGF) receptor, and that addition of NGF promotes the survival and neuronal commitment of BMC transplanted into the experimentally injured brain of littermates mice. Immunohistochemical analysis revealed that transplanted BMCs express tyrosine hydroxylase (TH) in proximity of the damaged dopaminergic tissues and choline acetyltransferase (ChAT) in the lesioned cholinergic regions. These results suggest that NGF supports the survival and differentiation of uncommitted BMCs and concurs with other local environmental signals to promote the expression of neuronal markers in these cells. The possible functional significance of these observations will be discussed.
Triaca
Viviana
V
National Research Council (C.N.R.), Institute of Neurobiology and Molecular Medicine V. 1e Marx, 15/43, 00137 Rome, Italy.
Aloe
Luigi
L
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007051:Ibotenic Acid / Q000633:toxicity*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D009498:Neurotoxins / Q000633:toxicity*; D016627:Oxidopamine / Q000633:toxicity*; D011247:Pregnancy; D020917:Receptor, trkA / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D009498:Neurotoxins; D007051:Ibotenic Acid; D016627:Oxidopamine; D020932:Nerve Growth Factor; D014446:Tyrosine 3-Monooxygenase; D020917:Receptor, trkA; D001973:Bromodeoxyuridine