Ovarian monocyte progenitor cells: phenotypic and functional characterization.
Leukocytes of the macrophage lineage are abundant in the ovarian tissues and have an important function in both follicular development and regression of postovulatory follicles. In this study, we tested the hypothesis that continuous production of macrophages in the ovarian stroma is maintained by a resident population of progenitors. We established a long-term culture of ovarian follicular stromal cells from BALB/c and green fluorescent protein-transgenic (GFP-TG) C57BL/6 mice. Nonadherent cells were collected and tested for hematopoietic function in vitro and in vivo. Histological and ultrastructural analyses revealed a homogenous population of monocyte-like rounded cells. Nonadherent cells continued to proliferate in culture for several months without senescence. When plated at very low density in methylcellulose, these cells formed colonies consisting of monocyte-like cells. Ovarian monocyte-like cells reacted with CD45, CD11b, CD11c, and Ly6-Gr-1 cell surface markers. A distinct CD45low population within these cells reacted with CD117 (C-kit) surface marker, suggestive of a primitive hematopoietic progenitor. Fifty thousand nonadherent cells failed to provide radioprotection to lethally irradiated mice and thus were not considered to be equivalent to pluripotent hematopoietic stem cells. Ovarian nonadherent stromal cells were positive for alkaline phosphatase but lacked embryonic cell antigens stage-specific embryonic antigen (SSEA-1) and Oct-4. We conclude that in the ovaries, a higher requirement for macrophages is provided by a resident stromal population of progenitors whose progeny is restricted to the production of cells of the monocyte-macrophage lineage.
Pascual
Cherry J
CJ
Department of Pediatrics, University of South Florida, St. Petersburg, FL 33612, USA.
Sanberg
Paul R
PR
Chamizo
Wilfredo
W
Haraguchi
Soichi
S
Lerner
Danika
D
Baldwin
Margi
M
El-Badri
Nagwa S
NS
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D039481:CD11b Antigen / Q000096:biosynthesis; D039521:CD11c Antigen / Q000096:biosynthesis; D002448:Cell Adhesion; D019070:Cell Lineage; D049109:Cell Proliferation; D017690:Cell Transplantation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D007962:Leukocytes / Q000166:cytology; D016256:Lewis X Antigen / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008747:Methylcellulose / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3; D010053:Ovary / Q000166:cytology* / Q000473:pathology; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000096:biosynthesis
D039481:CD11b Antigen; D039521:CD11c Antigen; D004268:DNA-Binding Proteins; D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D008747:Methylcellulose; D019009:Proto-Oncogene Proteins c-kit; D000469:Alkaline Phosphatase; D017493:Leukocyte Common Antigens