Involvement of sortase anchoring of cell wall proteins in biofilm formation by Streptococcus mutans.
Streptococcus mutans is one of the best-known biofilm-forming organisms associated with humans. We investigated the role of the sortase gene (srtA) in monospecies biofilm formation and observed that inactivation of srtA caused a decrease in biofilm formation. Genes encoding three putative sortase-dependent proteins were also found to be up-regulated in biofilms versus planktonic cells and mutations in these genes resulted in reduced biofilm biomass.
Lévesque
Céline M
CM
Oral Microbiology, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, Ontario, Canada M5G 1G6.
Voronejskaia
Elena
E
Huang
Yi-Chen Cathy
YC
Mair
Richard W
RW
Ellen
Richard P
RP
Cvitkovitch
Dennis G
DG
D020816:Amino Acid Motifs; D019881:Aminoacyltransferases / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002352:Carrier Proteins / Q000235:genetics; D002473:Cell Wall / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D037102:Lectins; D013295:Streptococcus mutans / Q000502:physiology*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D037102:Lectins; C037937:glucan-binding proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases