title: [Charactierization of a new antibacterial polypeptide isolated from human cervical mucas].
abstract: OBJECTIVE
  To identify a new antibactrial polypeptide HCP-1 isolated from human cervical mucus.
  
  
  METHODS
  HCP-1 was isolated and purified from human cervical mucus, and N-terminal sequence of HCP-1 was determined. A degenerate primer was designed according to CodeHop methods, and an "anchor-oliga-dT primer" was used for the synthesis of cDNA. Using the degenerate primer and anchor-primer, cDNAs were amplified by PCR. The PCR products were cloned, sequenced, and analyzed by biological software.
  
  
  RESULTS
  N-terminat sequence of HCP-1 was PKRKAEGDAK. The full length of HCP-1 cDNA was isolated and of which the sequence was the same as HMG-17. OMIGA software analysis indicated that this molecule contained an alpha-helix region.
  
  
  CONCLUSIONS
  The new antibacterial polypeptide isolated from human cervical mucus is HMG-17. It may play a role in the human cervical mucus and the alpha-helix domain may be its antibacterial activity region.
authors.affiliation: Research Unit of Infection & Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Pan
authors.forename: Li-li
authors.initials: LL
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Xun
authors.initials: X
authors.lastname: Li
authors.forename: Bo-yao
authors.initials: BY
authors.lastname: Wang
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002582:Cervix Mucus / Q000737:chemistry* / Q000502:physiology; D005260:Female; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011484:Protein-Arginine N-Methyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012097:Repressor Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D012097:Repressor Proteins; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases