CYP94A1, a plant cytochrome P450-catalyzing fatty acid omega-hydroxylase, is selectively induced by chemical stress in Vicia sativa seedlings.
CYP94A1 is a cytochrome P450 (P450) catalyzing fatty acid (FA) omega-hydroxylation in Vicia sativa seedlings. To study the physiological role of this FA monooxygenase, we report here on its regulation at the transcriptional level (Northern blot). Transcripts of CYP94A1, as those of two other P450-dependent FA hydroxylases (CYP94A2 and CYP94A3) from V. sativa, are barely detectable during the early development of the seedlings. CYP94A1 transcripts, in contrast to those of the two other isoforms, are rapidly (less than 20 min) and strongly (more than 100 times) enhanced after treatment by clofibrate, an hypolipidemic drug in animals and an antiauxin (p-chlorophenoxyisobutyric acid) in plants, by auxins (2,4-dichlorophenoxyacetic acid and indole-3-acetic acid), by an inactive auxin analog (2,3-dichlorophenoxyacetic acid), and also by salicylic acid. All these compounds activate CYP94A1 transcription only at high concentrations (50-500 microM range). In parallel, these high levels of clofibrate and auxins modify seedling growth and development. Therefore, the expression of CYP94A1 under these conditions and the concomitant morphological and cytological modifications would suggest the implication of this P450 in a process of plant defense against chemical injury.
Benveniste
Irène
I
CNRS-Institut de Biologie Molèculaire des Plantes--UPR2357--Département Réponses métaboliques à l'environnement biotique, Université Louis-Pasteur, 28 Rue Goethe, 67083, Strasbourg Cedex, France. Irene.Benveniste@bota-ulp.strasbg.fr
Bronner
Roberte
R
Wang
Yong
Y
Compagnon
Vincent
V
Michler
Pierre
P
Schreiber
Lukas
L
Salaün
Jean-Pierre
JP
Durst
Francis
F
Pinot
Franck
F
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002994:Clofibrate / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006540:Herbicides / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology; D013997:Time Factors; D033022:Vicia sativa / Q000187:drug effects* / Q000201:enzymology*
D006540:Herbicides; D007210:Indoleacetic Acids; D012333:RNA, Messenger; D015084:2,4-Dichlorophenoxyacetic Acid; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C426839:cytochrome P-450 CYP94A1 (Vicia sativa); C431910:cytochrome P-450 CYP94A2 (Vicia sativa); D002994:Clofibrate; D020156:Salicylic Acid