Effect of ultrasonic exposure on Ca2+-ATPase activity in plasma membrane from Aloe arborescens callus cells.
We investigated the effect of ultrasound on plasma membrane (PM) Ca2+-ATPase activity of Aloe arborescens callus cells in solid culture. The calluses were exposed by a 20 kHz digital sonifier at the powers of 2 and 10 W from the effective exposure times of 2-10 s. PM Ca2+-ATPase activity was almost significantly higher at 2 W both in continuous wave and 10% duty cycle than that of the control (no ultrasound) at effective exposure times of 5 and 10 s. However, its activity decreased at 10 W in continuous wave exposure. It is possible that the PM Ca2+-ATPase configuration or structure may be partly damaged by high-energy ultrasound at 10 W. Our results showed that low-energy ultrasound exposure was a useful physical field to stimulate A. arborescens callus cells to adapt environmental stress through PM Ca2+-ATPase activity increase.
Liu
Yiyao
Y
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, PR China. liuyiyao@hotmail.com
Yang
Hong
H
Takatsuki
Hideyo
H
Sakanishi
Akio
A
D000504:Aloe / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D014465:Ultrasonics
D000252:Calcium-Transporting ATPases