Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011.
Marine organisms are potentially an untapped source of drugs and value-added food production. Currently, Acetes chinensis is an underutilized shrimp species with low commercial value from the Bo Hai Gulf of China. In this paper, the shrimp was digested by a crude protease from Bacillus sp. SM98011 and filtered through a 3 kDa ultrafiltration membrane. Biological functions of the hydrolysate and ultrafiltrate were then assayed. The analyses showed that 41% of the oligopeptides in the ultrafiltrate had a molecular mass lower than 3 kDa. The antioxidant activities of the hydrolysate and ultrafiltrate were determined through the scavenger activity of the hydroxyl radical, with inhibitions of 42.38% and 67.95%, respectively. The hydrolysate and ultrafiltrate also had good Angiotensin-I-converting enzyme (ACE) inhibitory activity, with IC50 values of 0.98 mg/ml and 0.22 mg/ml, respectively. In addition, Chitin and chitosan were recovered from the hydrolytic sediment using a much smaller volume of strong acids and bases than is normally needed. With this method, we have shown that A. chinensis can be utilized to generate a high value-added product, and have revealed its hidden potential in the production of functional foods and ACE inhibitory peptides.
He
Hailun
H
State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, China.
Chen
Xiulan
X
Sun
Caiyun
C
Zhang
Yuzhong
Y
Gao
Peiji
P
D000596:Amino Acids / Q000032:analysis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism*; D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D002686:Chitin / Q000032:analysis; D048271:Chitosan / Q000032:analysis; D033364:Decapoda / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D014783:Viscosity
D000596:Amino Acids; D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; D002686:Chitin; D048271:Chitosan; D010450:Endopeptidases