Utilization of methyloleate in production of microbial lipase.
In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports.
Destain
Jacqueline
J
Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, B-5030 Gembloux, Belgium. destain.j@fsagx.ac.be
Fickers
Patrick
P
Weekers
Frédéric
F
Moreau
Benoît
B
Thonart
Philippe
P
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D004789:Enzyme Activation; D008049:Lipase / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D019301:Oleic Acid / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism*; D025062:Yarrowia / Q000201:enzymology* / Q000254:growth & development*
D009829:Oleic Acids; D000069463:Olive Oil; D010938:Plant Oils; D019301:Oleic Acid; C005576:methyl oleate; D008049:Lipase