PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.
Here we show that the PIASy protein is specifically required for mitotic modification of Topoisomerase-II by SUMO-2 conjugation in Xenopus egg extracts. PIASy was unique among the PIAS family members in its capacity to bind mitotic chromosomes and recruit Ubc9 onto chromatin. These properties were essential, since PIASy mutants that did not bind chromatin or failed to recruit Ubc9 were functionally inactive. We observed that PIASy depletion eliminated essentially all chromosomal accumulation of EGFP-SUMO-2-conjugated species, suggesting that it is the primary E3-like factor for mitotic chromosomal substrates of SUMO-2. PIASy-dependent SUMO-2-conjugated species were highly concentrated on the inner centromere, and inhibition of PIASy blocked anaphase sister chromatid segregation in egg extracts. Taken together, our observations suggest that PIASy is a critical regulator of mitotic SUMO-2 conjugation for Topoisomerase-II and other chromosomal substrates, and that its activity may have particular relevance for centromeric functions required for proper chromosome segregation.
Azuma
Yoshiaki
Y
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892-5431, USA.
Arnaoutov
Alexei
A
Anan
Tadashi
T
Dasso
Mary
M
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D020090:Chromosome Segregation / Q000502:physiology; D002875:Chromosomes / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT; D017434:Protein Structure, Tertiary; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism; D014981:Xenopus; D000098843:Ubiquitin-Conjugating Enzyme UBC9
D002843:Chromatin; D047908:Intracellular Signaling Peptides and Proteins; C485891:PIAS4 protein, human; D000075223:Poly-ADP-Ribose Binding Proteins; D050828:Protein Inhibitors of Activated STAT; C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D044763:Ubiquitin-Conjugating Enzymes; D004250:DNA Topoisomerases, Type II; D000098843:Ubiquitin-Conjugating Enzyme UBC9