title: Wt1 is not essential for hematopoiesis in the mouse.
abstract: WT1 has been implicated in human leukemia and hematopoiesis, but its role in stem cell differentiation is not yet fully defined. We show that Wt1-null murine fetal liver cells are capable of reconstituting functional hematopoiesis following transplantation into irradiated recipients. There was also no significant difference between the in vitro colony-forming ability of wild-type and Wt1-null cells. Using a reporter gene assay in a transgenic mouse system, expression from the WT1 promoter was detectable in adult bone marrow, but undetectable in subsets of different hematopoietic cells. We conclude that Wt1 is not essential for murine hematopoiesis and that there may be significant differences in its role between mouse and man.
authors.affiliation: Section of Paediatric Oncology, Institute of Cancer Research, 15 Cotswold Road, Belmont, Sutton, Surrey SM2 5NG, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: King-Underwood
authors.forename: Suzanne
authors.initials: S
authors.lastname: Little
authors.forename: Mandy
authors.initials: M
authors.lastname: Baker
authors.forename: Robyn
authors.initials: R
authors.lastname: Clutterbuck
authors.forename: Sylvie
authors.initials: S
authors.lastname: Delassus
authors.forename: Tariq
authors.initials: T
authors.lastname: Enver
authors.forename: Clive
authors.initials: C
authors.lastname: Lebozer
authors.forename: Toon
authors.initials: T
authors.lastname: Min
authors.forename: Adrian
authors.initials: A
authors.lastname: Moore
authors.forename: Andreas
authors.initials: A
authors.lastname: Schedl
authors.forename: Kathy
authors.initials: K
authors.lastname: Pritchard-Jones
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D025721:WT1 Proteins / Q000172:deficiency / Q000235:genetics*
chemical_list: D025721:WT1 Proteins