title: Comparative study of four extraction methods for enterovirus recovery from wastewater and sewage sludge.
abstract: This study investigated four methods for the recovery of enteroviruses from sterilized raw wastewater, activated sludge, thickened sludge and treated wastewater, inoculated with Echovirus 11, Gregory prototype. The adsorption-elution method recommended by the US Environmental Protection Agency (EPA) was better for Echovirus 11 recovery than a sonication method, a modified EPA method and a membrane adsorption elution method since it resulted in the highest detection levels by cell culture and RT-PCR (Friedman's test, p<0.00041 and p=0.041, respectively).
authors.affiliation: Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie de Monastir, 5000 Rue Avicenne, Monastir, Tunisia.
authors.forename: Khaoula
authors.initials: K
authors.lastname: Belguith
authors.forename: Abdennaceur
authors.initials: A
authors.lastname: Hassen
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
mesh_terms: D002478:Cells, Cultured; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000821:virology*; D014776:Virus Cultivation; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
chemical_list: D012722:Sewage