Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo.
The heat shock cognate 70-4 protein gene promoter (HSC70-4p) from Bombyx mori (BmHSC70-4p) is an ideal candidate for the transgenic silkworm due to its high transcriptional activity, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. Using luciferase as a reporter gene and transient expression system in vivo and in vitro, we found that BmNPVhr3 can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Moreover, the transcriptional activity of the combination of BmHSC70-4p and BmNPVhr3 changed with developmental stages and hormone titers. These results suggest that the combination of BmHSC70-4 promoter and BmNPVhr3 enhancer is more effective candidate for the transgene or stable cell expression system in Bombyx mori.
Tang
Shunming
S
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang.
Zhao
Qiaoling
Q
Yi
Yongzhu
Y
Zhang
Zhifang
Z
Li
Yiren
Y
D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D007605:Juvenile Hormones / Q000502:physiology; D007814:Larva; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013997:Time Factors; D014158:Transcription, Genetic
D006360:Heat-Shock Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones