title: A noninvasive oral rinse assay to monitor engraftment, neutrophil tissue delivery and susceptibility to infection following HSCT in pediatric patients.
abstract: The time interval between neutrophil tissue delivery and blood confirmed engraftment following hematopoietic stem cell transplantation (HSCT) may serve as an indicator of patient susceptibility to infection. Using an oral rinse protocol, we studied neutrophil tissue delivery kinetics and its relationship to clinical parameters post-HSCT in 29 pediatric patients. Oral neutrophil counts were compared to circulating neutrophil levels, oral mucositis scores and infection-related febrile episodes after engraftment. Blood engraftment (BE) is currently defined by a blood neutrophil count of > or =0.5 x 10(9)/l. We defined oral engraftment (OE) as the day neutrophils returned in the mouth post-HSCT (> or =0.25 x 10(4)/ml oral neutrophils in the rinse sample). We found that neutrophils reappeared 6.3+/-3.9 s.d. days earlier in the mouth than in the circulation enabling us to identify successful engraftment almost 1 week sooner than using blood count values alone. Furthermore, the time-span between OE and BE was inversely related to the number of infection-related febrile episodes post-BE. We conclude that monitoring the timing of neutrophil tissue delivery through a rapid oral rinse may yield important insights into the biology of neutrophil recovery during and after engraftment and the factors associated with neutrophil tissue recruitment.
authors.affiliation: CIHR Group in Matrix Dynamics, University of Toronto, Toronto, ON, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Cheretakis
authors.forename: Y
authors.initials: Y
authors.lastname: Dror
authors.forename: M
authors.initials: M
authors.lastname: Glogauer
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D004198:Disease Susceptibility; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007239:Infections / Q000175:diagnosis; D007700:Kinetics; D007938:Leukemia / Q000628:therapy*; D007958:Leukocyte Count / Q000379:methods*; D008297:Male; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000473:pathology; D009067:Mouthwashes; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
chemical_list: D009067:Mouthwashes