Intracellular redox state regulation by parthenolide.
In the present paper, we report a strong intracellular antioxidant activity of the sesquiterpene lactone parthenolide in the hippocampal HT22 cells. This effect is mediated by an increase of total glutathione at both, low (5 microM) and high (10 microM), concentrations. Parthenolide also increases the activation of the antioxidant/electrophile response element. This effect is the possible mediator of glutathione increase, since the limiting enzyme on its synthesis possesses this response element on its promoter. Finally, we demonstrate that its antioxidant properties do not mediate its antiproliferative effect nor its inhibition of NF-kappaB.
Herrera
Federico
F
Departamento de Morfología y Biología Celular, Facultad de Medicina, 33006 Oviedo, Spain.
Martin
Vanesa
V
Rodriguez-Blanco
Jezabel
J
García-Santos
Guillermo
G
Antolín
Isaac
I
Rodriguez
Carmen
C
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D007424:Intracellular Fluid / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D012717:Sesquiterpenes / Q000008:administration & dosage*
D000975:Antioxidants; D012717:Sesquiterpenes; C002669:parthenolide; D005978:Glutathione