title: Detection of fragmented genomic DNA by PCR-free piezoelectric sensing using a denaturation approach.
abstract: Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Firenze, 50019 Sesto Fiorentino, Italy. minunni@unifi.it
authors.forename: Maria
authors.initials: M
authors.lastname: Minunni
authors.forename: Sara
authors.initials: S
authors.lastname: Tombelli
authors.forename: Jessica
authors.initials: J
authors.lastname: Fonti
authors.forename: Maria M
authors.initials: MM
authors.lastname: Spiriti
authors.forename: Marco
authors.initials: M
authors.lastname: Mascini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bogani
authors.forename: Marcello
authors.initials: M
authors.lastname: Buiatti
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D014026:Nicotiana / Q000235:genetics
chemical_list: D018744:DNA, Plant; D004277:DNA, Single-Stranded; D009841:Oligonucleotides