title: Study on the characteristics of pectin-ketoprofen for colon targeting in rats.
abstract: Pectin-ketoprofen (PT-KP) prodrug with the potential for colon targeted delivery has been evaluated. A sensitive HPLC method was established for the determination of concentration of ketoprofen (KP) in rats. This method was also used to evaluate the colon targeting property of PT-KP. KP or PT-KP was given to rats by oral administration at a dosage of 10mg/kg. Plasma and the different parts of gastrointestinal (GI) tract were taken after 2, 4, 6, 8, 10 and 12h of oral administration of KP or PT-KP to rats and the concentration of KP was measured by HPLC. Preliminary experiments show KP distributes mainly in stomach, proximal small intestine and distal small intestine. However, KP released from PT-KP mainly distributes in cecum and colon. Therefore, this approach suggests that PT-KP prodrug has a good colon targeting property.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Miao Miao
authors.initials: MM
authors.lastname: Xi
authors.forename: San Qi
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Xin Yi
authors.initials: XY
authors.lastname: Wang
authors.forename: Kun Quan
authors.initials: KQ
authors.lastname: Fang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism*; D005079:Excipients; D005260:Female; D007660:Ketoprofen / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D010368:Pectins / Q000008:administration & dosage*; D011355:Prodrugs / Q000493:pharmacokinetics*; D051381:Rats
chemical_list: D005079:Excipients; D011355:Prodrugs; D010368:Pectins; D007660:Ketoprofen