The ingestion of Ginkgo biloba extract (EGb 761) inhibits arachidonic acid-mediated platelet aggregation and thromboxane B2 production in healthy volunteers.
Twelve non-diabetic volunteers (age = 39 +/- 13 years, BMI = 23.5 +/- 3.5) undertook a randomized double-blind placebo-controlled crossover study in which they ingested either 120 mg of EGb 761 or a placebo daily for 3 months and then switched to the other test capsules for the next 3 months. Platelet aggregation in platelet-rich plasma (PRP) was performed at the end of each 3-month arm, with or without 1-min incubation with graded doses of EGb 761. In the placebo cycles, AA-stimulated TXB2 production was 2581 +/-1337 pg/10(6) platelets (range 897-5485) compared to 1668 +/- 992 pg/10(6) platelets (range 6-1668) in the EGb 761 cycles (p < 0.005). Incubation of PRP with EGb 761 (150 microg/ml) completely inhibited platelet aggregation accompanied by inhibition of TXB2 synthesis in all subjects both in the placebo (<200 pg TXB2/10(6) platelets) and EGb 761 cycles (< 120 TXB2/10(6) platelets) (p < 0.0001). These results support EGb 761-mediated inhibition of platelet TXB2 synthesis in vivo.
Kudolo
George B
GB
Department of Clinical Laboratory Sciences, MSC 6246, University of Texas HSC at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA. Kudolo@uthscsa.edu
Wang
Wen
W
Barrientos
Jessica
J
Elrod
Ryan
R
Blodgett
Janet
J
D000328:Adult; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors / Q000097:blood*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D005260:Female; D020441:Ginkgo biloba; D006801:Humans; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000037:antagonists & inhibitors / Q000097:blood*; D013997:Time Factors; D000096983:Ginkgo Extract
D001095:Arachidonic Acids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013929:Thromboxane B2; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract