title: Behavioral changes following antisense oligonucleotide-induced reduction of organic cation transporter-3 in mice.
abstract: The organic cation transporter-3 (OCT3) can transport monoamines, similar to neuronal monoamine transporters. Due to the lack of selective ligands, however, the functional role of OCT3 is still unknown. Thus, we investigated behavioral effects of antisense against OCT3 (AS) in mice. AS (0.075-0.25 microg/0.25 microl/h, for 7 days) dose-dependently decreased immobility time. Moreover, although neither AS (0.075 microg/0.25 microl/h, for 7 days) or imipramine (4 mg/kg, i.p.) were effective, imipramine (4 mg/kg, i.p.) significantly decreased immobility time in mice treated with AS (0.075 microg/0.25 microl/h, for 7 days). Additionally, AS (0.25 microg/0.25 microl/h, for 7 days) significantly increased locomotor activity induced by methamphetamine (1 mg/kg, s.c.), but did not affect spontaneous locomotor activity. These results suggest that OCT3 might become a novel molecular target to treat depression and other diseases related to monoaminergic neuronal systems.
authors.affiliation: Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20, Daikominami, Nagoya 461-8673, Japan. kitaichi@met.nagoya-u.ac.jp
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Kitaichi
authors.forename: Masaya
authors.initials: M
authors.lastname: Fukuda
authors.forename: Hironao
authors.initials: H
authors.lastname: Nakayama
authors.forename: Nagisa
authors.initials: N
authors.lastname: Aoyama
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Yohei
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Takagi
authors.forename: Kenzo
authors.initials: K
authors.lastname: Takagi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects*; D015153:Blotting, Western; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007099:Imipramine / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D029362:Organic Anion Transporters, Sodium-Independent / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D013550:Swimming / Q000523:psychology
chemical_list: D000929:Antidepressive Agents, Tricyclic; D000697:Central Nervous System Stimulants; D016376:Oligonucleotides, Antisense; D029362:Organic Anion Transporters, Sodium-Independent; C114338:organic anion transport protein 3; D008694:Methamphetamine; D007099:Imipramine