title: Overview on sugar metabolism and its control in Lactococcus lactis - the input from in vivo NMR.
abstract: The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780-156 Oeiras, Portugal.
authors.forename: Ana Rute
authors.initials: AR
authors.lastname: Neves
authors.forename: Wietske A
authors.initials: WA
authors.lastname: Pool
authors.forename: Jan
authors.initials: J
authors.lastname: Kok
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Helena
authors.initials: H
authors.lastname: Santos
mesh_terms: D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism*; D013294:Lactococcus lactis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods
chemical_list: D019344:Lactic Acid; D000431:Ethanol