title: In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds.
abstract: A proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 x 10(-9) M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica(88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced approximately 34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets.
authors.affiliation: Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Carina L
authors.initials: CL
authors.lastname: Araújo
authors.forename: Ingrid W L
authors.initials: IW
authors.lastname: Bezerra
authors.forename: Adeliana S
authors.initials: AS
authors.lastname: Oliveira
authors.forename: Fabiano T
authors.initials: FT
authors.lastname: Moura
authors.forename: Leonardo L P
authors.initials: LL
authors.lastname: Macedo
authors.forename: Carlos E M
authors.initials: CE
authors.lastname: Gomes
authors.forename: Aulus E A D
authors.initials: AE
authors.lastname: Barbosa
authors.forename: Francisco P
authors.initials: FP
authors.lastname: Macedo
authors.forename: Tánia M S
authors.initials: TM
authors.lastname: Souza
authors.forename: Octavio L
authors.initials: OL
authors.lastname: Franco
authors.forename: Carlos
authors.initials: C
authors.lastname: Bloch-J
authors.forename: Mauricio P
authors.initials: MP
authors.lastname: Sales
mesh_terms: D033622:Ceratitis capitata; D004591:Electrophoresis, Polyacrylamide Gel; D007306:Insecticides; D012639:Seeds / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029861:Tamarindus / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000302:isolation & purification; D048889:Weevils
chemical_list: D007306:Insecticides; D014361:Trypsin Inhibitors