Influence of metabolic stress on the inheritance of cell determination in the moss, Pottia intermedia.
Epigenetically-determined apogamy in aposporous regenerants of the moss Pottia intermedia persists during vegetative propagation, the capacity of apogamy being inherited by individual aposporous protonemal cells. To test Bauer-Lazarenko's proposal that stable apogamy in mosses may be due to some self-replicating cytoplasmic factor, the effects of different metabolic stress treatments on the expression of apogamy have been tested. Chronic metabolic stress caused by long-term growth of autotrophic aposporous protonema on mineral medium with 0.25% of casamino acids and on Murashiga-Skoog (MS) medium with sucrose and phytohormones, as well as by transitory action of high kinetin concentration, have a much stronger influence on the expression of apogamy, than short-term stress treatments with RNase and Pb(2+). Apogamy has been found to be lost stably, after prolonged growth on MS medium containing kinetin and ABA. The proposal that the capacity for apogamy is related to the release of aposporous protonemal cells from a putative factor for apogamy is discussed.
Lobachevska
O
O
Institute of Ecology of the Carpathians, National Academy of Sciences of Ukraine, 11 Stefanyk Street, 79000 Lviv, Ukraine. bryology@bryology.lviv.ua
Kyjak
N
N
Khorkavtsiv
O
O
Dovgalyuk
A
A
Kit
N
N
Klyuchivska
O
O
Stoika
R
R
Ripetsky
R
R
Cove
D
D
D000040:Abscisic Acid; D000225:Adenine / Q000031:analogs & derivatives / Q000378:metabolism; D019068:Bryopsida / Q000254:growth & development* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007701:Kinetin; D010937:Plant Growth Regulators / Q000378:metabolism; D030821:Plants, Genetically Modified; D012100:Reproduction, Asexual; D012260:Ribonucleases / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D010937:Plant Growth Regulators; D013395:Sucrose; D000040:Abscisic Acid; D012260:Ribonucleases; D000225:Adenine; D007701:Kinetin