title: Genetic variation of exon 2 of SLA-DQB gene in Chinese pigs.
abstract: A 273 base pair (bp) fragment of the SLA-DQB gene including parts of intron 1 and exon 2 has been investigated using PCR-RFLP in 38 indigenous Chinese pig breeds, two Chinese wild boars, and three foreign pig breeds. The restriction enzyme RsaI revealed three polymorphic sites in the 273 bp fragment for the pig breeds studied. In total, four alleles resulting in 10 genotypes were found. Twenty pig breeds are not in Hardy-Weinberg equilibrium at this locus. The allele frequency of a chi-square test showed that there is significant difference (P < 0.05) among six Chinese pig groups, and an even greater significant difference (P < 0.01) was found between Chinese and European pig breeds.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Fang
authors.forename: Martin
authors.initials: M
authors.lastname: Braunschweig
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Hu
authors.forename: Luosui
authors.initials: L
authors.lastname: Hu
authors.forename: Jidong
authors.initials: J
authors.lastname: Feng
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Changxin
authors.initials: C
authors.lastname: Wu
mesh_terms: D000483:Alleles; D000818:Animals; D002681:China; D005787:Gene Frequency; D005802:Genes, MHC Class II; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*