title: Susceptibility of B lymphocytes to adenovirus type 5 infection is dependent upon both coxsackie-adenovirus receptor and alphavbeta5 integrin expression.
abstract: Human lymphocytes are resistant to genetic modification, particularly from recombinant adenoviruses, thus hampering the analysis of gene function using adenoviral vectors. This study engineered an Epstein-Barr virus-transformed B-lymphoblastoid cell line permissive to adenovirus infection and elucidated key roles for both the coxsackie-adenovirus receptor and alphavbeta5 integrin in mediating entry of adenoviruses into these cells. The work identified a strategy for engineering B cells to become susceptible to adenovirus infection and showed that such a strategy could be useful for the introduction of genes to alter lymphoblastoid-cell gene expression.
authors.affiliation: Infection and Immunity, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Ciarán
authors.initials: C
authors.lastname: Richardson
authors.affiliation: Infection and Immunity, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Brennan
authors.affiliation: Infection and Immunity, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Powell
authors.affiliation: Infection and Immunity, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Stuart
authors.initials: S
authors.lastname: Prince
authors.affiliation: Biomolecular Sciences Building, School of Biology, University of St Andrews, St Andrews, UK.
authors.forename: Yun-Hsiang
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Virus Receptor and Immune Evasion Group, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff, UK.
authors.forename: O Brad
authors.initials: OB
authors.lastname: Spiller
authors.affiliation: Infection and Immunity, Henry Wellcome Research Building, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Rowe
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000378:metabolism / Q000502:physiology*; D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D016023:Integrins; D011991:Receptors, Virus; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5