Highly sensitive analysis of the anti-tumor agent 1-[4-(furo[2,3-b]-quinolin-4-ylamino)phenyl]ethanone in rat plasma by high-performance liquid chromatography using electrochemical detection.
A sensitive high-performance liquid chromatography method with electrochemical detection was developed for the purpose of determining the concentration of the new anti-tumor agent 1-[4-(furo[2,3-b]-quinolin-4-ylamino)phenyl]ethanone (FQPE) in rats. The plasma samples were spiked with the internal standard diclofenac and extracted using dichloromethane. A C(18) 250 mm x 4mm column was used for the separation of analyte with a mobile phase consisting of 50% acetonitrile and 50% pH 3.0 of sodium 1-pentansulfonate solution at a flow rate of 1.0 mL/min. FQPE was detected by electrochemical detector at 1.0 V and 20 nA. Intra-day and inter-day precision and accuracy were acceptable down to the limit of quantization of 1 ng/mL. The lower limit of detection (LOD) was 0.5 ng/mL. The pharmacokinetic parameters of FQPE in rats after intravenous administration of 2.1 and 4.2 mg/kg were determined. The apparent volume of distribution, half-life of elimination, and clearance showed no significant difference between the two dosages. The area under the plasma concentration time curve increased proportionally with dose. The half-life of FQPE was prolonged about 2.4-fold, compared with amsacrine.
Huang
Yaw-Bin
YB
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung City, 80708 Taiwan, R.O.C.
Wu
Pao-Chu
PC
Hsu
Ming-Wei
MW
Chen
Yeh-Long
YL
Tzeng
Cherng-Chyi
CC
Tsai
Yi-Hung
YH
D000677:Amsacrine / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004563:Electrochemistry / Q000379:methods*; D006207:Half-Life; D015394:Molecular Structure; D011804:Quinolines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods
C510259:1-(4-(furo(2,3-b)quinolin-4-ylamino)phenyl)ethanone; D000970:Antineoplastic Agents; D011804:Quinolines; D000677:Amsacrine