Identification of leukemia-associated antigens in chronic myeloid leukemia by proteomic analysis.
The immune system plays an important role in the treatment of chronic myeloid leukemia (CML). Identification of leukemia-associated antigens (LAAs) eliciting an immune response in patients is a prerequisite for specific immunotherapy of CML. To identify new LAAs in CML, We utilized a novel approach based serology and proteomics technologies. LAAs were identified by comparing the reactivity of proteins resolved by 2-DE with sera from CML patients and healthy donors. Several new LAAs were identified including alpha enolase, aldolase A, HSP70 protein8, beta-tubulin and tropomyosin isoforms. Although, the functions of these identified proteins in CML need further investigation, the detection of autoantibodies in CML may have value on CML screening, diagnosis, or follow-up. Additionally, identification of LAAs in CML may also be of vital importance in antigen-based immunotherapy.
Zou
Liyun
L
Institute of Immunology of PLA, Third Military Medical University, Chongqing 400038, PR China.
Wu
Yuzhang
Y
Pei
Li
L
Zhong
Daping
D
Gen
Miao
M
Zhao
Tingting
T
Wu
Jiehong
J
Ni
Bing
B
Mou
Zhirong
Z
Han
Junfeng
J
Chen
Yongwen
Y
Zhi
Yi
Y
D000293:Adolescent; D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D001323:Autoantibodies / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D014335:Tropomyosin / Q000032:analysis; D014404:Tubulin / Q000032:analysis
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins; D014335:Tropomyosin; D014404:Tubulin; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase