Genomic DNA amplification from a single bacterium.
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.
Raghunathan
Arumugham
A
Molecular Staging, Inc., New Haven, Connecticut, USA.
Ferguson
Harley R
HR
Bornarth
Carole J
CJ
Song
Wanmin
W
Driscoll
Mark
M
Lasken
Roger S
RS
D017103:Bacillus Phages / Q000201:enzymology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D048168:Escherichia coli K12 / Q000166:cytology* / Q000235:genetics; D005434:Flow Cytometry; D005821:Genetic Techniques; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000166:cytology / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004259:DNA-Directed DNA Polymerase