title: Application of HPLC for determination of phytic acid in the colonic epithelial Caco-2 cells.
abstract: Phytic acid (myo-inositol hexaphosphate, IP6) is currently receiving a considerable interest because of its anticancer (preventive and therapeutic) potential against colon tumors and the need for methods of its determination. The aim of this study was to analyze the uptake of IP6 by human colon adenocarcinoma cells (Caco-2 cell line) and to evaluate the method of its intracellular quantification with the use of high performance liquid chromatography (HPLC) technique. Chromatographic analysis revealed a rapid uptake of IP6 by cells. The intracellular accumulation of IP6 was saturable at 0.5 h and it did not change with the prolongation of incubation up to 72 h.
authors.affiliation: Department of Biochemistry, Medical University of Silesia 1 Narcyzów Str., 41-200 Sosnowiec, Poland.
authors.forename: Ludmiła
authors.initials: L
authors.lastname: Weglarz
authors.forename: Beata
authors.initials: B
authors.lastname: Parfiniewicz
authors.forename: Beata
authors.initials: B
authors.lastname: Bat
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Orchel
authors.forename: Zofia
authors.initials: Z
authors.lastname: Dzierzewicz
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
mesh_terms: D018938:Caco-2 Cells; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010833:Phytic Acid / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010833:Phytic Acid