title: A high-resolution radiation hybrid map of bovine chromosome 5q1.3-q2.5 compared with human chromosome 12q.
abstract: In this study we present a comprehensive 3000-rad radiation hybrid map on bovine chromosome 5 (BTA5) of a region between 12.8 and 74.0 cM according to the linkage map, which contains a quantitative trait loci for ovulation rate. We mapped 28 gene-associated sequence tagged site markers derived from sequences of bovine BAC clones and 10 microsatellite markers to the BTA5 region. In comparison with HSA12q, four blocks of conserved synteny were apparent showing three chromosomal breakpoints and two inversions in this segment of BTA5. Therefore, we have improved breakpoint resolution in the human-bovine comparative map, which enhances the determination of candidate genes underlying traits of interest mapped to BTA5.
authors.affiliation: Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17p, 30559 Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Mömke
authors.forename: C
authors.initials: C
authors.lastname: Drögemüller
authors.forename: O
authors.initials: O
authors.lastname: Distl
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D019295:Computational Biology; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D010060:Ovulation / Q000235:genetics; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci; D021101:Radiation Hybrid Mapping; D016324:Sequence Tagged Sites
chemical_list: D017931:DNA Primers