[An interactive volume rendering algorithm for laser scanning confocal microscope data].
OBJECTIVE
To satisfy different observational demands, a real-time interactive volume rendering for laser scanning confocal microscope (LSCM) image was realized on the PC.


METHODS
An interactive transfer function was proposed through analyzing optical properties of LSCM image, so that the users can enroll in exploring the LSCM data set by altering rendering parameters. The texture mapping based on visualization method is adopted to realize real-time 3D reconstruction.


RESULTS
The algorithm was realized on the common PC. All 3D reconstruction results, which fulfills various demands, was obtained by the above algorithm with LSCM data set sized 256 x 256 x 40 pixels. In the process of reconstruction, the object could be re-rendered at a rate of 10 frames per second when the rendering parameters were adjusted.


CONCLUSIONS
Different from the traditional algorithms, the one we demonstrate here is able to offer a real-time environment on the PC and to get the 3D reconstruction results, thus easily achieve diverse observational mapping.
Chen
Ji
J
Hong
Di-hui
DH
Zheng
Xiao-xiang
XX
D000465:Algorithms; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007834:Lasers; D018613:Microscopy, Confocal; D014584:User-Computer Interface