title: Immunohistochemical detection of cdc2 is useful in predicting survival in patients with mantle cell lymphoma.
abstract: Recent cDNA microarray studies have reported the prognostic value of several genes in mantle cell lymphoma patients. We aimed to validate the prognostic significance of three of these genes: alpha-tubulin, cdc2, and CENP-F. The protein expression of alpha-tubulin, cdc2, and CENP-F was assessed using immunohistochemistry. Their immunoreactivity in 48 formalin-fixed/paraffin-embedded mantle cell lymphoma tumors was determined by estimating the percentage of positive cells. These results were correlated with the expression of proliferation marker Ki67 and survival. Of these 48 mantle cell lymphoma patients, 41 were men and seven were women. The median age at time of diagnosis was 64.5 years, and the overall median survival was 40 months. In benign lymph nodes, the expression of cdc2 and alpha-tubulin was restricted to the germinal centers; mantle zones were negative. Expression of CENP-F was more uniformly distributed. In mantle cell lymphoma, Ki67 significantly correlated with all three markers (P<0.05, Spearman), but only Ki67 (>50%) and cdc2 (>25%) significantly correlated with shorter survival (P<0.0006, Spearman). Of several clinical parameters examined, international prognostic index of >or=2 correlated with worse clinical outcome, and high clinical stage (ie 4 vs <or=3) showed a trend for shorter survival. The prognostic significance of cdc2 and Ki67 was independent of international prognostic index and clinical stage. We have validated the prognostic value of cdc2, and confirmed that of Ki67, in a cohort of mantle cell lymphoma patients. Immunohistochemical detection of cdc2 and Ki67 may be a useful and simple method in evaluating the prognosis of mantle cell lymphoma patients.
authors.affiliation: Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Hui
authors.forename: Tony
authors.initials: T
authors.lastname: Reiman
authors.forename: John
authors.initials: J
authors.lastname: Hanson
authors.forename: Rick
authors.initials: R
authors.lastname: Linford
authors.forename: Winson
authors.initials: W
authors.lastname: Wong
authors.forename: Andrew
authors.initials: A
authors.lastname: Belch
authors.forename: Raymond
authors.initials: R
authors.lastname: Lai
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D016203:CDC2 Protein Kinase / Q000096:biosynthesis*; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D020522:Lymphoma, Mantle-Cell / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D008297:Male; D008840:Microfilament Proteins; D008875:Middle Aged; D011379:Prognosis; D015203:Reproducibility of Results; D016019:Survival Analysis; D014404:Tubulin / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; D002868:Chromosomal Proteins, Non-Histone; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D014404:Tubulin; C094772:centromere protein F; D016203:CDC2 Protein Kinase