title: Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti.
abstract: The effect of gene expression knockdown was used to study the function of the sterol carrier protein-2 (AeSCP-2) in the yellow fever mosquito, Aedes aegypti. Injection of small double stranded AeSCP-2 RNAs into mosquito larvae resulted in the knockdown of gene products. The lack of AeSCP-2 in larvae coincided with a reduction in accumulated cholesterol in pupae, supporting the hypothesis that AeSCP-2 may be involved in cholesterol uptake in mosquito larvae. Knockdown of AeSCP-2 caused a high mortality rate in developing adult and reduced egg viability. Results from this study indicate that AeSCP-2 is important for adult development and for the viability of the eggs.
authors.affiliation: Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Blitzer
authors.forename: I
authors.initials: I
authors.lastname: Vyazunova
authors.forename: Q
authors.initials: Q
authors.lastname: Lan
mesh_terms: D000330:Aedes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D020868:Gene Silencing; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007814:Larva / Q000378:metabolism; D009154:Mutation; D010063:Ovum; D011679:Pupa / Q000378:metabolism; D012098:Reproduction
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; C072087:sterol carrier proteins; D002784:Cholesterol