title: Development of Isospora belli in Hct-8, Hep-2, human fibroblast, BEK and Vero culture cells.
abstract: The development of Isospora belli, a human coccidian parasite, was studied in different cell lines. Merozoites were observed in all kinds of cells, whereas sporogony was demonstrated only in Hct-8. This implied that not only the human cell line can be infected, but also some animal cell lines. Unizoites could be found in Vero cells. The merozoites were transferred to a new culture cell for three passages and maintained for two weeks, but no oocyst production was observed in any culture cells during cultivation.
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmcsr@mahidol.ac.th
authors.forename: Chutatip
authors.initials: C
authors.lastname: Siripanth
authors.forename: Benjanee
authors.initials: B
authors.lastname: Punpoowong
authors.forename: Pornsawan
authors.initials: P
authors.lastname: Amarapal
authors.forename: Niramol
authors.initials: N
authors.lastname: Thima
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005243:Feces / Q000469:parasitology; D005347:Fibroblasts / Q000469:parasitology; D006790:Host-Parasite Interactions; D006801:Humans; D007549:Isospora / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D013045:Species Specificity; D034101:Sporozoites / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014709:Vero Cells