In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model.
OBJECTIVE
This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model.


METHODS
In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37 degrees C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37 degrees C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test.


RESULTS
There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P = 0.237).


CONCLUSIONS
The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P = 0.047), suggesting an inferior adhesion potential for this microorganism.
Lima
L M
LM
Department of Pediatric Dentistry, Araraquara Dental School, University of São Paulo State, Rua Humaitá, 1680, Avenida Mariangela Pucci Ananias, 305 Araraquara, Brazil. lulima@yahoo.com
Motisuki
C
C
Spolidorio
D Madalena Palomari
DM
Santos-Pinto
L
L
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D003731:Dental Caries / Q000382:microbiology* / Q000517:prevention & control; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D007779:Lactobacillus acidophilus / Q000502:physiology*; D007780:Lacticaseibacillus casei / Q000502:physiology*; D019936:Probiotics; D013295:Streptococcus mutans / Q000472:pathogenicity; D013696:Temperature; D013997:Time Factors