title: Indian primary HIV-2 isolates and relationship between V3 genotype, biological phenotype and coreceptor usage.
abstract: The chemokine coreceptors play a significant role in HIV entry and pathogenesis. The V3 region of HIV envelope glycoprotein is considered as a principal determinant for viral phenotype and tropism. The present study describes lack of association between the V3 genotype and viral phenotype of 18 Indian HIV-2 isolates. The viruses were isolated, confirmed by PCR and the HIV subtypes were determined by sequencing V3 region of the env gene. The coreceptor usage and syncytium inducing (SI) capacity of isolates was determined. Our study indicated that CCR5 coreceptor usage and NSI phenotype is predominant among Indian HIV-2 isolates obtained from patients in the early stage of infection. Two of the four HIV-2 isolates obtained from the late stage patients were SI and dual tropic. Phylogenetic analysis of these isolates revealed close relatedness to the isolates from western and southern India.
authors.affiliation: Department of Molecular Virology, National AIDS Research Institute, Bhosari, India. skulkarni@nariindia.org
authors.forename: Smita
authors.initials: S
authors.lastname: Kulkarni
authors.forename: Srikanth
authors.initials: S
authors.lastname: Tripathy
authors.forename: Kalpana
authors.initials: K
authors.lastname: Agnihotri
authors.forename: Neena
authors.initials: N
authors.lastname: Jatkar
authors.forename: Sushama
authors.initials: S
authors.lastname: Jadhav
authors.forename: Winston
authors.initials: W
authors.lastname: Umakanth
authors.forename: Kavita
authors.initials: K
authors.lastname: Dhande
authors.forename: Prasad
authors.initials: P
authors.lastname: Tondare
authors.forename: Raman
authors.initials: R
authors.lastname: Gangakhedkar
authors.forename: Ramesh
authors.initials: R
authors.lastname: Paranjape
mesh_terms: D002460:Cell Line; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology; D006679:HIV Seropositivity / Q000276:immunology*; D015498:HIV-2 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D019713:Receptors, CCR5 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D019713:Receptors, CCR5