Interaction of the protein phosphatase 2A with the regulatory domain of the cystic fibrosis transmembrane conductance regulator channel.
A direct interaction of the regulatory domain (R domain) of the cystic fibrosis transmembrane conductance regulator protein (CFTR) with PR65, a regulatory subunit of the protein phosphatase 2A (PP2A), was shown in yeast two hybrid, pull-down and co-immunoprecipitation experiments. The R domain could be dephosphorylated by PP2A in vitro. Overexpression of the interacting domain of PR65 in Caco-2 cells, as well as treatment with okadaic acid, showed a prolonged deactivation of the chloride channel. Taken together our results show a direct and functional interaction between CFTR and PP2A.
Vastiau
Annick
A
Department of Human Genetics, Division of Human Mutations and Polymorphisms, KULeuven, Herestraat 49, Postbus 602, 3000 Leuven, Belgium.
Cao
Lishuang
L
Jaspers
Martine
M
Owsianik
Grzegorz
G
Janssens
Veerle
V
Cuppens
Harry
H
Goris
Jozef
J
Nilius
Bernd
B
Cassiman
Jean-Jacques
JJ
D000595:Amino Acid Sequence; D000818:Animals; D018938:Caco-2 Cells; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D054648:Protein Phosphatase 2; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C505032:CFTR protein, human; D021122:Protein Subunits; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2