Myogenic program induction in mature fat tissue (with MyoD expression).
MyoD exerts a master transcriptional control over the myogenic differentiation cascade. Here, we study different approaches to induce myogenic transdifferentiation in mature adipocytes utilizing MyoD gene transfer. Organotypic cultures of fat tissue and a long-term culture of in vitro differentiated adipocytes deduced that MyoD provoked morphological changes in mature adipocytes that can be summarized as loss of fat content, acquisition of a fusiform shape and eventual fusion with committed neighbor cells. In vivo, MyoD gene transfer into rat interscapular and inguinal fat pads demonstrated that while structural proteins of muscle lineage were expressed, they co-existed with specific adipocyte proteins. Expression of these proteins diminished over time likewise the fat content. The transdifferentiation process initiated by MyoD did not require cell cycle progression and was well tolerated by the fully differentiated and mature adipocytes.
Kocaefe
Y C
YC
Genethon CNRS UMR8115 1 bis rue de l'Internationale 91002 Evry cedex, France. kocaefe@hacettepe.edu.tr
Israeli
D
D
Ozguc
M
M
Danos
O
O
Garcia
L
L
D000256:Adenoviridae / Q000235:genetics; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism; D017570:MyoD Protein / Q000096:biosynthesis / Q000235:genetics*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics; D019459:Urothelium / Q000166:cytology / Q000378:metabolism
D015415:Biomarkers; D009124:Muscle Proteins; D017570:MyoD Protein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins