title: Magnetic resonance tracking of human CD34+ progenitor cells separated by means of immunomagnetic selection and transplanted into injured rat brain.
abstract: Magnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell visualization. Human cells from peripheral blood were selected by CliniMACS CD34 Selection Technology (Miltenyi). Purified CD34+ cells were implanted into rats with a cortical photochemical lesion, contralaterally to the lesion. Twenty-four hours after grafting, the implanted cells were detected in the contralateral hemisphere as a hypointense spot on T2 weighted images; the hypointensity of the implant decreased during the first week. At the lesion site we observed a hypointensive signal 10 days after grafting that persisted for the next 3 weeks, until the end of the experiment. Prussian blue and anti-human nuclei staining confirmed the presence of magnetically labeled human cells in the corpus callosum and in the lesion 4 weeks after grafting. CD34+ cells were also found in the subventricular zone (SVZ). Human DNA (a human-specific 850 base pair fragment of alpha-satellite DNA from human chromosome 17) was detected in brain tissue sections from the lesion using PCR, confirming the presence of human cells. Our results show that CD34 microbeads superparamagnetic nanoparticles can be used as a magnetic cell label for in vivo cell visualization. The fact that microbeads coated with different commercially available antibodies can bind to specific cell types opens extensive possibilities for cell tracking in vivo.
authors.affiliation: Institute of Experimental Medicine ASCR, Prague, Czech Republic. pavla.jendelova@lfmotol.cuni.cz
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
authors.forename: Vít
authors.initials: V
authors.lastname: Herynek
authors.forename: Lucia
authors.initials: L
authors.lastname: Urdziková
authors.forename: Katerina
authors.initials: K
authors.lastname: Glogarová
authors.forename: Sárka
authors.initials: S
authors.lastname: Rahmatová
authors.forename: Ivan
authors.initials: I
authors.lastname: Fales
authors.forename: Benita
authors.initials: B
authors.lastname: Andersson
authors.forename: Pavel
authors.initials: P
authors.lastname: Procházka
authors.forename: Josef
authors.initials: J
authors.lastname: Zamecník
authors.forename: Tomás
authors.initials: T
authors.lastname: Eckschlager
authors.forename: Petr
authors.initials: P
authors.lastname: Kobylka
authors.forename: Milan
authors.initials: M
authors.lastname: Hájek
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008863:Microspheres; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D020521:Stroke / Q000628:therapy*; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; D004247:DNA