[Establishment of the model of heterotopic uterine transplantation in syngeneic rats].
OBJECTIVE
To develop a surgical method for establishment of a heterotopic uterine transplantation model in syngeneic rats and evaluate its feasibility.


METHODS
Thirty pairs of Wistar rats aged 8 - 10 weeks were used as donor and recipient. Heterotopic uterine transplantation was conducted, and the duration of the operation was recorded. The recipient rats were killed 7 days after surgery. The morphology of the transplanted uteri was evaluated.


RESULTS
Thirty heterotopic uterine transplantations were conducted. The survival rate increased from 40% (6/15) in the first 15 pairs of recipient rats to 75% (12/15) in the last 15 pairs of recipient rats. Among the 18 living recipient rats, 15 transplanted uteri were viable. The viable rate of uterine transplantation was 83%. Compared with the first 15 pairs of rats, the duration of donor procedures, recipient procedures vascular anastomosis and the total time of the surgery decreased to (65 +/- 10) min, (89 +/- 22) min, (36 +/- 8) min and (154 +/- 23) min respectively in the last 15 pairs of rats.


CONCLUSIONS
It is feasible to establish the model of heterotopic uterine transplantation in Wistar rats.
Yan
Qin
Q
Department of Obstetrics and Gynecology, Affiliated First People's Hospital, Jiaotong University, Shanghai 200080, China.
Wan
Xiao-ping
XP
He
Xiao-ying
XY
Xi
Xiao-wei
XW
Yang
Yi-xia
YX
D000818:Animals; D005260:Female; D006801:Humans; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016378:Tissue Transplantation / Q000379:methods* / Q000401:mortality; D016042:Transplantation, Heterotopic / Q000379:methods / Q000401:mortality; D014599:Uterus / Q000601:surgery* / Q000637:transplantation*