[Primary study on the influence of blood transfusion on the STR genotypes of recipient's blood].
OBJECTIVE
To investigate whether any change occurred in recipients' blood collected at different times after transfusion with different quantity of blood.


METHODS
Three patients were transfused with 400 ml, 800 ml and 1200 ml blood separately. The blood samples were collected from the recipients before transfusion and at 4 h, 8 h, 12 h after transfusion, and from the donors. DNA were extracted by Chelex-100 method and were amplified by the polymerase chain reaction (PCR). Six loci of D1S549, D18S865, D3S1754, D12S391, D12S375, D6S477 were selected. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis and silver staining.


RESULTS
The investigations on the six STR loci revealed that the patients' STR genotypes remained unchanged within 12 h after blood transfusion.


CONCLUSIONS
The STR genotypes of the donors would have no influence on the STR genotypes of the patients within 12 h after transfusion providing the volume of blood transfused is less than 1200 ml.
Han
Jun-ge
JG
Department of Forensic Biology, West China School of Preclincal and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Guo-di
GD
Liu
Zhi-yong
ZY
Yu
Lin
L
Liao
Miao
M
Wu
Mei-yun
MY
Liao
Zhi-gang
ZG
Kang
Yan
Y
Luo
Chuan-xin
CX
Jin
Xiao-dong
XD
D044466:Asian People; D001803:Blood Transfusion; D004247:DNA / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D020080:Tandem Repeat Sequences / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA