Insulin-degrading enzyme degrades amyloid peptides associated with British and Danish familial dementia.
Familial British dementia (FBD) and familial Danish dementia (FDD) are autosomal dominant disorders characterized by cerebrovascular and parenchymal amyloid deposition and neurofibrillary degeneration. In both conditions, the genetic defects cause the loss of the normal stop codon in the precursor BRI, generating novel 34-residue peptides named ABri and ADan in FBD and FDD, respectively. ABri and ADan show a strong tendency to aggregate into non-fibrillar and fibrillar structures at neutral pH and this property seems to be directly related to neurotoxicity. Here we report that a recombinant insulin-degrading enzyme (rIDE) was capable of degrading monomeric ABri and ADan in vitro more efficiently than oligomeric species. These peptides showed high beta-structure content and were more resistant to proteolysis as compared to the BRI wild-type product of 23 amino acids. Specific sites of cleavage within the C-terminal pathogenic extensions raise the possibility that proteolysis of monomeric soluble precursors by IDE may delay ABri and ADan aggregation in vivo.
Morelli
Laura
L
Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, (CONICET), Patricias Argentinas 435, Buenos Aires 1405, Argentina.
Llovera
Ramiro E
RE
Alonso
Leonardo G
LG
Frangione
Blas
B
de Prat-Gay
Gonzalo
G
Ghiso
Jorge
J
Castaño
Eduardo M
EM
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D003545:Cysteine / Q000737:chemistry; D003704:Dementia / Q000235:genetics* / Q000378:metabolism*; D003718:Denmark; D005799:Genes, Dominant; D006801:Humans; D066298:In Vitro Techniques; D007339:Insulysin / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D020836:Protein Structure, Quaternary; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D006113:United Kingdom
D048868:Adaptor Proteins, Signal Transducing; D000682:Amyloid; C120083:ITM2B protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D007339:Insulysin; D003545:Cysteine