Meniscal repair using bone marrow-derived mesenchymal stem cells: experimental study using green fluorescent protein transgenic rats.
Meniscal tears in the avascular zone have very limited potential to heal because of a poor blood supply. Although there have been many attempts to promote the healing potential of the torn meniscus, no established treatments have achieved sufficient meniscal healing. In this study, we evaluated the efficacy of mesenchymal stem cell transplantation as a cell source to promote meniscal healing, using cells from the green fluorescent protein (GFP) transgenic rat and organ culture model. Mesenchymal stem cells from bone marrow were isolated and expanded in monolayer culture. They were embedded in fibrin glue and were transplanted into the meniscal defects of Sprague-Dawley rats. In the control groups, the defects remained untreated, or only fibrin glue without cells was transplanted. The GFP-positive cells enabled us to detect the transplanted cells from recipient cells easily. As a result, transplanted mesenchymal stem cells could survive and proliferate in the meniscal defects in the organ culture model. They also could produce an abundant extracellular matrix stained by toluidine blue around the cells which contributed to meniscal healing in the avascular status. We could detect transplanted GFP cells under a fluorescent microscope until 8 weeks after transplantation. In a clinical situation, mesenchymal stem cell transplantation is a promising new clinical strategy for the treatment of meniscal tears in the avascular zone.
Izuta
Yasunori
Y
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, 734-8551, Japan. izuta@hiroshima-u.ac.ip
Ochi
Mitsuo
M
Adachi
Nobuo
N
Deie
Masataka
M
Yamasaki
Takuma
T
Shinomiya
Rikuo
R
D000818:Animals; D030801:Animals, Genetically Modified; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism; D015718:Fibrin Tissue Adhesive; D005347:Fibroblasts / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006490:Hemostatics; D007718:Knee Injuries / Q000628:therapy*; D049408:Luminescent Agents / Q000378:metabolism*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley; D000070600:Tibial Meniscus Injuries
D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D049408:Luminescent Agents; D049452:Green Fluorescent Proteins