title: Regulation of anxiety and initiation of sexual behavior by CREB in the nucleus accumbens.
abstract: Sexual deficits and other behavioral disturbances such as anxiety-like behaviors can be observed in animals that have undergone social isolation, especially in species having important social interactions. Using a model of protracted social isolation in adult rats, we observed increased anxiety-like behavior and deficits in both the latency to initiate sexual behavior and the latency to ejaculate. We show, using transgenic cAMP response element (CRE)-LacZ reporter mice, that protracted social isolation also reduces CRE-dependent transcription within the nucleus accumbens. This decrease in CRE-dependent transcription can be mimicked in nonisolated animals by local viral gene transfer of a dominant negative mutant of CRE-binding protein (CREB). We previously showed that this manipulation increases anxiety-like behavior. We show here that it also impairs initiation of sexual behavior in nonisolated animals, a deficit that can be corrected by anxiolytic drug treatment. This local reduction in CREB activity, however, has no influence on ejaculation parameters. Reciprocally, we used the viral transgenic approach to overexpress CREB in the nucleus accumbens of isolated animals. We show that this local increase in CREB activity completely rescued the anxiety phenotype of the isolated animals, as well as their deficit in initiating sexual behavior, but failed to rescue the deficit in ejaculation. Our data suggest a role for the nucleus accumbens in anxiety responses and in specific aspects of sexual behavior. The results also provide insight into the molecular mechanisms by which social interactions affect brain plasticity and behavior.
authors.affiliation: Department of Psychiatry and Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9070, USA. mbarrot@neurochem.u-strasbg.fr
authors.forename: Michel
authors.initials: M
authors.lastname: Barrot
authors.forename: Deanna L
authors.initials: DL
authors.lastname: Wallace
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Bolaños
authors.forename: Danielle L
authors.initials: DL
authors.lastname: Graham
authors.forename: Linda I
authors.initials: LI
authors.lastname: Perrotti
authors.forename: Rachael L
authors.initials: RL
authors.lastname: Neve
authors.forename: Heather
authors.initials: H
authors.lastname: Chambliss
authors.forename: Jerry C
authors.initials: JC
authors.lastname: Yin
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Nestler
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001007:Anxiety / Q000503:physiopathology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009714:Nucleus Accumbens / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012726:Sexual Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D012934:Social Isolation; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D014151:Anti-Anxiety Agents; D017362:Cyclic AMP Response Element-Binding Protein