[The expression of cytokines in experimental otitis media with effusion].
OBJECTIVE
To explore the relationship between type I allergic reaction and OME and the expression of Th1, Th2 cytokines in the middle ear mucosa in OME.


METHODS
BALB/C mice were randomly divided into experimental group (Exp), control group I (Con1) and control group II (Con2), BALB/C mice were sensitized to ovalbumin (OVA) and challenged transtympanically. The thickness of mucosa, the infiltration of eosinophils were detected by hematotoxylin-eosin staining (HE staining), the expression of IL-4, IL-5, IFN-gamma and IL-12 were examined by immunohistochemistry.


RESULTS
In experimental group, the expression of IL-4, IL-5, the thicknesss of mucosa and the numbers of eosinophils were significantly higher than those of Con1, Con2. But the expression of IFN-gamma, IL-12 were both significantly low. The concentration of IL-5 were positively correlated with the number of eosinophils in mucosa.


CONCLUSIONS
This study demonstrates that type I allergic reaction, Th2 cytokines have an important effect on OME.
Sun
Rong
R
Department of Otorhinolaryngology, the First Hospital, Chongqing Medical University, Chongqing, 400016, China. sunr2002@eyou.com
Hong
Suling
S
Tang
Xinye
X
D000818:Animals; D006969:Hypersensitivity, Immediate / Q000150:complications* / Q000276:immunology / Q000473:pathology; D015847:Interleukin-4 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D051379:Mice; D010034:Otitis Media with Effusion / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D011897:Random Allocation; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D015848:Interleukin-5; D015847:Interleukin-4