title: Correlation of CENP-F gene expression with tumor-proliferating activity in human salivary gland tumors.
abstract: We examined the expression of Centromere protein F (CENP-F) mRNA in 26 human salivary gland tumors (seven pleomorphic adenomas, three Warthin tumors, seven mucoepidermoid carcinomas, four adenoid cystic carcinomas, four acinic cell carcinomas and one malignant myoepithelioma) and four normal submandibular glands using the real time quantitative reverse transcription-polymerase chain reaction (RT-PCR). The mean expression level of CENP-F mRNA was higher in malignant tumors (1.05+/-0.32) than normal submandibular glands (0.11+/-0.05) and benign tumors (0.46+/-0.16). We found a significant association between the level of CENP-F mRNA expression and clinical stage in 16 malignant tumors (Mann-Whitney U test, p=0.027). We also found a significant correlation between the Ki-67 labeling index and CENP-F expression in malignant tumors (Spearmans correlation coefficient by rank test, p=0.029). These results indicate that human CENP-F mRNA is closely linked to the increased or abnormal cell proliferation in malignant conditions.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Shigeishi
authors.forename: Kuniko
authors.initials: K
authors.lastname: Mizuta
authors.forename: Koichiro
authors.initials: K
authors.lastname: Higashikawa
authors.forename: Shingo
authors.initials: S
authors.lastname: Yoneda
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Ono
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kamata
mesh_terms: D000236:Adenoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D008840:Microfilament Proteins; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000235:genetics*; D013363:Submandibular Gland / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D008840:Microfilament Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C094772:centromere protein F