title: Determination of sinomenine in human plasma by HPLC/ESI/ion trap mass spectrum.
abstract: BACKGROUND
  Sinomenine is a pure alkaloid with a variety of pharmacological actions including anti-inflammation, immuno-suppression, analgesia, antihypertension, and antiarrhythmia. Methods have been developed to measure sinomenine in rats and rabbits through high-performance liquid chromatography (HPLC). But only one report described a method for determination of sinomenine in humans.
  
  
  METHODS
  We developed a method to quantitate sinomenine in human plasma based on liquid chromatography-ion trap mass spectrometry coupled with electrospray ionization (HPLC/ESI/ion trap mass spectrum).
  
  
  RESULTS
  The calibration curve (r2=0.9993) of sinomenine was established with standard solutions. The mean inter-day and intra-day CVs<15%. The limit of detection for sinomenine was 0.5 ng/ml. The relative recovery of high, middle and low concentration was 99%, 103%, and 118%, respectively.
  
  
  CONCLUSIONS
  The method is simple, rapid, sensitive, specific, and accurate enough for determination of sinomenine in human plasma and may be used to investigate the metabolism and pharmacokinetics of sinomenine.
authors.affiliation: Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Yao
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Tan
authors.forename: Zhe-Yi
authors.initials: ZY
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Ze-Neng
authors.initials: ZN
authors.lastname: Cheng
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009019:Morphinans / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009019:Morphinans; C009271:sinomenine