Biological activity of recombinant chicken interleukin-6 in chicken hybridoma cells.
Interleukin-6 (IL-6), a multipotential cytokine that plays roles in regulating immune responses, acute phase reactions and hematopoiesis, induces proliferation and antibody production in hybridoma cells. The biological activities of the recombinant chicken IL-6 (rchIL-6) were determined using murine and chicken hybridoma cells. Cell proliferation and tyrosine phosphorylation of signal transducer and activator of transcription-3 (STAT3) were induced by rchIL-6 in the IL-6-dependent murine hybridoma cell line MH60, whereas the recombinant protein exhibited no significant cell proliferation activity in chicken hybridoma cells but induced antibody production and tyrosine phosphorylation of STAT3. The lack of cell proliferation induced by rchIL-6 in HUC2-13 cells may have been because the cell line was not IL-6-dependent in contrast to MH60 cells. These results suggest that rchIL-6 may be useful for promoting antibody production of chicken hybridoma cells as well as for creating chicken hybridomas by cell fusion.
Nishimichi
Norihisa
N
Department of Molecular and Applied Biological Science, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan.
Aosasa
Masayoshi
M
Kawashima
Tsuyoshi
T
Horiuchi
Hiroyuki
H
Furusawa
Shuichi
S
Matsuda
Haruo
H
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D006825:Hybridomas / Q000187:drug effects* / Q000502:physiology; D007136:Immunoglobulins / Q000096:biosynthesis; D015850:Interleukin-6 / Q000494:pharmacology*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000494:pharmacology; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism
D004268:DNA-Binding Proteins; C016512:IgY; D007136:Immunoglobulins; D015850:Interleukin-6; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D015534:Trans-Activators