title: Expression and immunolocalisation of odorant-binding and chemosensory proteins in locusts.
abstract: We have identified, cloned and expressed a new chemosensory protein (CSP) in the desert locust Schistocerca gregaria belonging to a third sub-class of these polypeptides. Polyclonal antibodies stained a band of 14 kDa, as expected, in the extracts of antennae and palps of the adults, but not in the 4th and 5th instars. In the related species Locusta migratoria, instead, the same antibodies cross-reacted only with a band of apparent molecular mass of 35 kDa in the extract of 1st-5th instars, but not in the adults. The recombinant protein binds the fluorescent probe N-phenyl-1-naphthylamine, but none of the compounds so far reported as pheromones for S. gregaria. The expression of the odorant-binding protein (OBP) and of CSPs of sub-classes I and II was also monitored in antennae, tarsi, palpi, wings and other organs of solitary and gregarious locusts in their nymphal and adult stages. OBP was found to be antenna specific, where it is expressed at least from the 3rd instar in both solitary and gregarious locusts. CSPs, instead, appear to be more ubiquitous, with different expression patterns, according to the sub-class. Immunocytochemistry experiments revealed that OBP is present in the sensillum lymph of sensilla trichodea and basiconica, while CSP-I and CSP-III were found in the outer sensillum lymph of sensilla chaetica and in the sub-cuticular space between epidermis and cuticle of the antenna. Sensilla chaetica on other parts of the body showed the same expression of CSP-I as those on the antenna.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Brandazza
authors.forename: A
authors.initials: A
authors.lastname: Navarrini
authors.forename: L
authors.initials: L
authors.lastname: Ban
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: R A
authors.initials: RA
authors.lastname: Steinbrecht
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Pelosi
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D005456:Fluorescent Dyes / Q000378:metabolism; D015870:Gene Expression; D006110:Grasshoppers / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D048188:Locusta migratoria / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009758:Nymph / Q000737:chemistry / Q000378:metabolism; D018035:Receptors, Odorant / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D005456:Fluorescent Dyes; D019476:Insect Proteins; D008024:Ligands; D018035:Receptors, Odorant; C503050:chemosensory protein 3, Schistocerca gregaria; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine