Ercc6l, a gene of SNF2 family, may play a role in the teratogenic action of alcohol.
The expression profile of a newly identified mouse nucleotide excision repair (NER) gene, Ercc6l, was investigated in a mouse model of fetal alcohol syndrome (FAS). In test 1, whole-mount in situ hybridization showed Ercc6l expressed mainly in the neural tube and heart of 10.5-day embryo. However, the expressions in both of the two organs were significantly down regulated after in uterus alcohol exposure from embryonic day (ED) 6-10, which was in accordance with the result of semi-quantitative RT-PCR. In test 2, the dams were given alcohol intragastrically from ED 6-15, and Northern blot of Ercc6l mRNA was carried out with five major embryo organs on ED 15.5, which were heart, brain, kidney, liver and lung. Ercc6l expression in 15.5-day embryonic brain and heart, which are the most commonly affected organs of FAS, were both decreased by alcohol exposure. The expressions in the other three organs were unaffected. From the results, we considered that Ercc6l might play a role in the teratogenic action of alcohol.
Xu
Yajun
Y
Laboratory of Molecular Toxicology & Developmental Molecular Biology, Department of Nutrition & Food Hygiene, School of Public Health, Peking University, Beijing 100083, China.
Chen
Xianggui
X
Li
Yong
Y
D000428:Alcohol Drinking / Q000009:adverse effects; D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004265:DNA Helicases / Q000096:biosynthesis / Q000235:genetics*; D004260:DNA Repair; D045643:DNA Repair Enzymes; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D000075223:Poly-ADP-Ribose Binding Proteins; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013723:Teratogens / Q000633:toxicity*
D000075223:Poly-ADP-Ribose Binding Proteins; D013723:Teratogens; D000431:Ethanol; D012313:RNA; D004247:DNA; D004265:DNA Helicases; C513075:Ercc6 protein, mouse; D045643:DNA Repair Enzymes