Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila.
To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects >300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.
Blanchette
Marco
M
Department of Molecular and Cell Biology, Center for Integrative Genomics, Plant and Microbial Biology, University of California, Berkeley, 94720-3204 USA.
Green
Richard E
RE
Brenner
Steven E
SE
Rio
Donald C
DC
D000818:Animals; D001665:Binding Sites; D004331:Drosophila melanogaster / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012322:RNA Precursors; D012333:RNA, Messenger