[The effect of UV-irradiation on telomerase activity and other stress-related proteins in human lens epithelial cells].
OBJECTIVE
To investigate the changes and the role of telomerase activity and other stress-related proteins in the process of UV-induced DNA damage and repair in human lens epithelial cells.


METHODS
Human lens epithelial cells were irradiated at UV-doses 0.0 (control group) and 0.5, 1.5, 2.5, 3.5, 5.0, 7.5, 10.0 mJ/cm(2) (treated 1-7 group). Telomerase activity was determined by Telomerase Repeat Amplification Protocol-Enzyme Linked Immunosorbent Assay (TRAP-ELISA), p53, growth arrest and DNA damage inducible (GADD45), proliferating cell nuclear antigen (PCNA) and p16 protein levels were analyzed by Western blotting.


RESULTS
Telomerase activity in control group and treated 1-7 group showed increased tendency, the differences of telomerase activity in 8 groups were significantly (P < 0.01). The expression of p53, GADD45, PCNA, p16 proteins showed increased tendency in experimental group, comparing with the control group, there were significant difference (P < 0.01).


CONCLUSIONS
During UV-induced DNA damage and repair in human lens epithelial cells, telomerase activity was upregulated and the expression of stress-related proteins levels was increased. Upregulated telomerase activity may play both a protective and a proliferative role in human lens epithelial cells. Increased stress-related proteins level is critic in UV-induced DNA damage and repair in human lens epithelial. Increased telomerase activity is associated with increased levels of the stress-related proteins.
Wu
Zhi-hong
ZH
Department of Ophthalmology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China. fswuzhihong@sina.com
Zhang
Jin-song
JS
D002478:Cells, Cultured; D004249:DNA Damage; D004847:Epithelial Cells / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology*; D019098:Telomerase / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D000097642:GADD45 Proteins
D047908:Intracellular Signaling Peptides and Proteins; D019098:Telomerase; D000097642:GADD45 Proteins