title: [Effects of pentachlorophenol on rat sertoli cells].
abstract: OBJECTIVE
  To detect the toxic effects of pentachlorophenol (PCP) on cultured rat Sertoli cells.
  
  
  METHODS
  The viability of Sertoli cells was detected and morphological examination was performed, followed by flow cytometric assay to evaluate the toxic effect of PCP on rat Sertoli cells.
  
  
  RESULTS
  MTT assay showed that PCP induced a concentration- and time-dependent decrease in Sertoli cell viability. Flow cytometric assay revealed that the number of dead Sertoli cells grew along with increased exposure to PCP.
  
  
  CONCLUSIONS
  PCP, with obvious cytotoxic effects, can cause necrosis of Sertoli cells in vitro.
authors.affiliation: Laboratory of Immunology and Reproductive Biology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
authors.forename: Shu-zhen
authors.initials: SZ
authors.lastname: Yang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Da-qiang
authors.initials: DQ
authors.lastname: Yin
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008297:Male; D010416:Pentachlorophenol / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000502:physiology
chemical_list: D010416:Pentachlorophenol