Energy transduction by respiratory complex I--an evaluation of current knowledge.
Complex I (NADH:ubiquinone oxidoreductase) is a membrane-bound, multisubunit enzyme. At present, there is no high-resolution structural model available for complex I and its mechanism of energy transduction is unknown. However, the subunit compositions of complex I from both eukaryotic and prokaryotic organisms have been determined, the sequences of their subunits are known and a coherent picture of the redox cofactors present in complex I has been developed. Here, I aim to describe and examine data relating to the mechanism of complex I and to build a framework to facilitate the discussion of possible conclusions and mechanistic models.
Hirst
J
J
Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 2XY, UK. jh@mrc-dunn.cam.ac.uk
D000818:Animals; D001665:Binding Sites; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004735:Energy Transfer; D010084:Oxidation-Reduction; D013379:Substrate Specificity
D042967:Electron Transport Complex I