[Experimental study on polylactic glycolate acid microparticles with releasing-slowly vascular endothelial growth factor].
OBJECTIVE
To observe the degradation of the polylactic glycolate acid (PLGA) microparticles with releasing-slowly vascular endothelial growth factor (VEGF) synthesized by the method of emulsification-diffusion.


METHODS
The method of emulsification-diffusion is to incorporate VEGF into microparticles composed of biodegradable PLGA. The controlled release of microparticles are acquired. The content of the VEGF released slowly from PLGA microparticles in vitro was detected with ELISA at different time.


RESULTS
We synthesized 100 releasing-slowly VEGF PLGA microparticles with the size of 0.20-0.33 microm by 5 times. The contents were 62 +/- 11 ng/L, 89 +/- 14 ng/L, and 127 +/- 19 ng/L in the 1st, the 2nd and the 3rd months after degradation, respectively.


CONCLUSIONS
The PLGA microparticles with releasing-slowly VEGF can be synthesized by the method of emulsification-diffusion.
Chen
Yang
Y
Department of Orthopaedics, Shenzhen Second People's Hospital, Shenzhen Guangdong, 518035, PR China. chenyang@tom.com
Luo
Xinle
X
Xiao
Jiande
J
D003692:Delayed-Action Preparations / Q000737:chemistry; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
D003692:Delayed-Action Preparations; D004337:Drug Carriers; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D011100:Polyglycolic Acid; D019344:Lactic Acid