Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus thuringiensis.
The expression of vip3LB, one of the vegetative insecticidal protein-encoding genes of Bacillus thuringiensis, was studied at the transcriptional level. By primer extension analysis, we have identified, for the first time, the transcription start point of vip3-type gene. Upstream from vip3LB transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E sigma(E) holoenzyme of B. subtilis. Thus, it was strongly suggested that the identified vip3 promoter was under the control of sigma(35)-like enzyme, the B. thuringiensis homolog of sigma(E). The transcriptional activity from the promoter was detected at the vegetative stage of growth starting at mid-log phase as well as during the middle stage of sporulation.
Mesrati
Lobna Abdelkefi
LA
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia.
Tounsi
Slim
S
Kamoun
Fakher
F
Jaoua
Samir
S
D001413:Bacillus thuringiensis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D024363:Transcription Initiation Site
D001426:Bacterial Proteins