title: Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration.
abstract: BMP-2 (bone morphogenetic protein-2) has been used in promoting bone formation and, in combination with various delivery carriers, in bone regeneration or bone tissue-engineering practice. In the present study, BMP-2 was immobilized directly on a GBR (guided bone-regenerative) membrane surface made of chitosan nanofibres, providing a bioactive surface that can enhance bone-regeneration capacity. The BMP-2-conjugated membrane surface retained bioactivity for up to 4 weeks of incubation, as well as holding over 50% of the initial BMP-2 attached. The BMP-2-conjugated surface increased osteoblastic cell attachment in accordance with the amount of BMP-2 conjugated. In addition, the BMP-2-conjugated chitosan membrane significantly promoted cell proliferation, alkaline phosphatase activity, as well as calcium deposition when compared with BMP-2-adsorbed membrane, indicating a stable localization of BMP-2 at the surface of the chitosan membrane. Taking these findings together, the conjugation of BMP-2 on a chitosan nanofibrous membrane would seen to be applicable for inducing significant and localized bone formation in future GBR procedures.
authors.affiliation: Craniomaxillofacial Reconstructive Science Major, School of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul 110-749, South Korea.
authors.forename: Yoon Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Kyung Hwa
authors.initials: KH
authors.lastname: Kim
authors.forename: Jue Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Ku
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Byung Moo
authors.initials: BM
authors.lastname: Min
authors.forename: Chong Pyoung
authors.initials: CP
authors.lastname: Chung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry*; D001861:Bone Regeneration; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D048091:Guided Tissue Regeneration; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D036103:Nanotechnology; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D048271:Chitosan