title: Flow injection spectrofluorimetric study of the supramolecular interaction between beta-cyclodextrin and dequalinium chloride and its analytical application.
abstract: The supramolecular interaction of dequalinium chloride (DQC) and beta-cyclodextrin has been studied by flow injection spectrofluorimetry. The results showed that beta-CD reacted with DQC to form a 1:1 host:guest complex with an apparent association constant of 4.99 x 10(2) L mol(-1). Based on the enhancement of the fluorescence intensity of DQC, a flow injection spectrofluorometric method for the determination of DQC in bulk aqueous solution in the presence of beta-CD was developed. The linear range was 0.0412-30.00 microg mL(-1). The detection limit was 12.3 ng mL(-1) with a sampling rate of 80 h(-1). There was no interference from the excipients normally used in tablets and serum compositions. The proposed method was successfully applied to the determination of DQC in tablets and serum.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China. tangb@sdnu.edu.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Baoxiu
authors.initials: B
authors.lastname: Jia
authors.forename: Xifeng
authors.initials: X
authors.lastname: Shi
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003868:Dequalinium / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013607:Tablets / Q000737:chemistry; D013816:Thermodynamics; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D013607:Tablets; D047392:beta-Cyclodextrins; D003868:Dequalinium