title: [Clinical significance and relationship of expression of matrix metalloproteinase-2 mRNA with breast cancer metastasis].
abstract: OBJECTIVE
  To discuss the clinical significance of matrix metalloproteinase-2 (MMP-2) mRNA and the relationship of MMP-2 mRNA expression with breast cancer metastasis.
  
  
  METHODS
  Detect MMP-2 mRNA expression of 30 breast cancer, metastatic lymphnode and 116 clinical breast cancer samples by flurence-quantitative RT-PCR and analysis the relativity of MMP-2 mRNA expression with clinical, pathology factors.
  
  
  RESULTS
  MMP-2 mRNA expression in 30 metastatic lymphnode is lower than in primary breast cancer (t = -3.293, P < 0.05). The mRNA expression of MMP-2 in tumor > 2 cm is lower than in tumor </= 2 cm (t = 1.936, P < 0.05). The MMP-2 mRNA expression in stage II, III is lower than in stage I (t = 2.466, P < 0.05). The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1 - 3 positive (t = 3.202, P < 0.05). The MMP-2 mRNA expression is irrelated with tissue grade, pathologic type, ER, PR and Her-2.
  
  
  CONCLUSIONS
  MMP-2 mRNA expression is up-regulated in early clinic stage, lymphnode metastatic early stage, and down regulated with tumor progressing. MMP-2 mRNA expression is related with the metastasis of breast cancer.
authors.affiliation: Department of Breast, Tianjin Medical University Cancer Hospital, Tianjin 300060, China. Email: llxxqq80@163.com.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Xiao
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Feng
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Xi-Shan
authors.initials: XS
authors.lastname: Hao
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D020778:Matrix Metalloproteinase 2