Reviving the exosome.
In the nucleus of eukaryotic cells, RNA decay in the 3'-5' direction is carried out by a complex of exonucleases called the exosome. Surprisingly, the purified exosome shows only weak activity in vitro. Two papers in this issue of Cell, by LaCava et al. (2005) and Wyers et al. (2005), and a third report by Vanacova et al. (2005) help to solve this riddle by characterizing a new RNA decay-activating complex containing a poly(A) polymerase. In addition, they identify new unconventional RNA polymerase II transcription units.
Jensen
Torben Heick
TH
Department of Molecular Biology, Arhus University, 8000 Arhus C., Denmark.
Moore
Claire
C
D002467:Cell Nucleus / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism; D063326:Exosome Multienzyme Ribonuclease Complex; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D012319:RNA Polymerase II; D011062:Polynucleotide Adenylyltransferase; D004259:DNA-Directed DNA Polymerase; C487545:PAP2 protein, S cerevisiae; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C112610:RRP6 protein, S cerevisiae