Effect of every other day feeding diet on gene expression in normal and in long-lived Ames dwarf mice.
Ames dwarf mutant mice are long-lived, hypoinsulinemic and hypoglycemic and exhibit enhanced sensitivity to injected insulin. Their phenotypic characteristics show many similarities to animals subjected to caloric restriction (CR) but Ames dwarf mice are not CR mimetics. Reducing daily food intake by 30% prolongs longevity in both normal and Ames dwarf mice. In the present study, the animals were subjected to a different type of CR, every other day feeding (EOD). Using real-time PCR, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice after 9 months of EOD. The results indicate that EOD produces some changes in the insulin and IGF1 signaling pathways, and that these changes are consistent with EOD increasing insulin sensitivity.
Masternak
Michal M
MM
Departments of Internal Medicine and Physiology, Geriatrics Research, Southern Illinois University, School of Medicine, Springfield, IL 62794, USA. mmasternak@siumed.edu
Al-Regaiey
Khalid A
KA
Bonkowski
Michael S
MS
Panici
Jacob A
JA
Bartke
Andrzej
A
D000273:Adipose Tissue / Q000378:metabolism; D000375:Aging / Q000235:genetics; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000235:genetics; D031204:Caloric Restriction / Q000379:methods*; D004032:Diet; D015870:Gene Expression / Q000235:genetics*; D007328:Insulin / Q000097:blood / Q000235:genetics; D055504:Insulin Receptor Substrate Proteins; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000502:physiology; D008136:Longevity / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D047495:PPAR gamma / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D011972:Receptor, Insulin / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics
D001786:Blood Glucose; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; C526295:Irs1 protein, mouse; C526298:Irs2 protein, mouse; D047495:PPAR gamma; D010750:Phosphoproteins; D007334:Insulin-Like Growth Factor I; D011972:Receptor, Insulin