title: The short-term in situ model to evaluate the anticariogenic potential of ionomeric materials.
abstract: OBJECTIVE
  Aiming to contribute to the study of mechanisms involved in the anticariogenic properties of dental materials, this study assessed the suitability of a short-term in situ model to evaluate the anticariogenic potential of ionomeric materials.
  
  
  METHODS
  The study used a 3-phase crossover, double blind design, and in each phase eight volunteers wore palatal appliances containing four enamel blocks restored with one of the following materials: composite resin (CR-Z250) (negative control), a conventional glass ionomer cement (GIC-Ketac-Fil) or a resin-modified GIC (RM-GIC-Vitremer). The restored blocks were covered with a "test plaque" of S. mutans, placed in palatal appliances and a cariogenic challenge was made during 1 min with 20% sucrose solution. After 45 min, test plaque was collected for fluoride (F) analysis. Enamel surface microhardness was previously determined at one side of the restoration and the percentage of surface microhardness change (%SMC) in relation to baseline (other side) was calculated. F concentration in enamel was also evaluated.
  
  
  RESULTS
  Split-plot ANOVA showed a statistically lower %SMC on enamel around the ionomeric materials than around the CR (p<0.05). This result was supported by a statistically higher F concentration in test plaque (P<0.001) and in enamel (P<0.001) restored with the ionomeric materials when compared to the CR.
  
  
  CONCLUSIONS
  The results suggest that the short-term in situ model tested is useful for studying the anticariogenic potential of dental materials that release fluoride.
authors.affiliation: Faculty of Dentistry of Piracicaba, State University of Campinas, Av. Limeira 901, 13414-903 Piracicaba, SP, Brazil.
authors.forename: Livia Maria Andaló
authors.initials: LM
authors.lastname: Tenuta
authors.forename: Cecilia Claudia Costa
authors.initials: CC
authors.lastname: Ribeiro
authors.forename: Nilza Cristina de Valor
authors.initials: NC
authors.lastname: Gonçalves
authors.forename: Altair Antoninha
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: Carolina Patrícia
authors.initials: CP
authors.lastname: Aires
authors.forename: Cristiana
authors.initials: C
authors.lastname: Tengan
authors.forename: Elaine Pereira Silva
authors.initials: EP
authors.lastname: Tagliaferro
authors.forename: Giovana Daniela
authors.initials: GD
authors.lastname: Pecharki
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Cínthia Pereira Machado
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002417:Cattle; D003773:Dental Plaque / Q000737:chemistry; D004812:Epidemiologic Methods; D005260:Female; D005459:Fluorides / Q000627:therapeutic use*; D005899:Glass Ionomer Cements / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D017001:Tooth Demineralization / Q000517:prevention & control*
chemical_list: D002327:Cariostatic Agents; D005899:Glass Ionomer Cements; D005459:Fluorides