Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens.
Blood samples were collected from 98 horses suspected of having borreliosis or granulocytic ehrlichiosis in Connecticut and New York State, USA during 1985, 1995, and 1996. Serum antibodies to Borrelia burgdorferi were detected by an enzyme-linked immunosorbent assay (ELISA), based on whole-cell and recombinant antigens, in 82 (84%) horses. Of the 181 sera tested, 59% were positive, using whole-cell antigens, compared to 48% with protein (p)37 and 35% with VlsE antigens. An ELISA containing either of these fusion proteins can be used as an adjunct to general screening by an ELISA or immunoblotting in animals not vaccinated for this disease.
Magnarelli
L
L
The Connecticut Agricultural Experiment Station, P.O. Box 1106, New Haven, CT 06504, USA. louis.magnarelli@po.state.ct.us
Fikrig
E
E
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis* / Q000453:epidemiology; D006736:Horses; D008193:Lyme Disease / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D014481:United States / Q000453:epidemiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011994:Recombinant Proteins