title: Investigation of acute toxicity of chlorpyrifos-methyl on guppy Poecilia reticulata.
abstract: Static bioassays were made to determine acute toxicity of chlorpyrifos-methyl, a wide spectrum organophosphorus insecticide and potential toxic pollutant of aquatic ecosystem, Guppy fish (Poecilia reticulata). Bioassays were made at a regulated temperature of 22+/-1 degrees C and were repeated three times. Lethal doses of the insecticides were determined using LC50 software programme of U.S. EPA based on Finney's Probit Analysis statistical method. The 96 h LC50 value and 95% confidence limit of chlorpyrifos-methyl for Guppy was estimated as 1.79 (1.47-2.10) mg/l. The fish exposed to chlorpyrifos-methyl exhibited behavioral changes in the form of neurotoxin toxicity: less general activity than control group, loss of equilibrium, erratic swimming and staying motionless at a certain location generally at mid-water level for prolonged periods. The 1 mg/l (lowest) concentration had similar behavior (NOEC) with the control group.
authors.affiliation: Department of Biology Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Selvi
authors.forename: Rabia
authors.initials: R
authors.lastname: Sarikaya
authors.forename: Figen
authors.initials: F
authors.lastname: Erkoç
authors.forename: Oner
authors.initials: O
authors.lastname: Koçak
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001681:Biological Assay; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D011036:Poecilia / Q000502:physiology*; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl