Microdissection-derived murine mcb probes from somatic cell hybrids.
The multicolor-banding (mcb) technique is a fluorescence in situ hybridization (FISH)-banding approach, which is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first three available mcb-probe sets for the Mus musculus chromosomes 3, 6, and 18. In the present work, the creation of the microdissection libraries was done for the first time on mouse/human somatic cell hybrids. During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes.
Trifonov
Vladimir
V
Institut für Human Genetics and Anthropology, Postfach, D-07740 Jena, Germany.
Karst
Constanze
C
Claussen
Uwe
U
Mrasek
Kristin
K
Michel
Susanne
S
Avner
Philip
P
Liehr
Thomas
T
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000648:ultrastructure*; D006822:Hybrid Cells / Q000648:ultrastructure*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D042282:Microdissection