title: Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3.
abstract: We examined the metal ion cofactor preference for MST3 (mammalian Ste20-like kinase 3) of the Ste20 serine/threonine kinase family. Four metal ions (Mg(+2), Mn(+2), Zn(2+), and Co(2+)) activate endogenous, exogenous, and baculovirus-expressed recombinant MST3 within the physiological concentration range. In contrast, Fe(+2) and Ca(+2) do not function as MST3 cofactors. Mn(2+), Co(2+), and Mg(2+)-dependent autophosphorylation of MST3 is mainly on threonine residue while Zn(2+)-stimulated MST3 autophosphorylation is on both serine and threonine residues. The distinct autophosphorylation pattern on MST3 suggests that MST3 may exert various types of kinase reactions depending on the type of metal ion cofactor used. To our knowledge, this is the first report showing Zn(2+) as the metal ion cofactor of a recombinant serine/threonine kinase.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan.
authors.forename: Te-Jung
authors.initials: TJ
authors.lastname: Lu
authors.forename: Chi-Ying F
authors.initials: CY
authors.lastname: Huang
authors.forename: Chiun-Jye
authors.initials: CJ
authors.lastname: Yuan
authors.forename: Yuan-Chii
authors.initials: YC
authors.lastname: Lee
authors.forename: Tzeng-Horng
authors.initials: TH
authors.lastname: Leu
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Chang
authors.forename: Te-Ling
authors.initials: TL
authors.lastname: Lu
authors.forename: Wen-Yih
authors.initials: WY
authors.lastname: Jeng
authors.forename: Ming-Derg
authors.initials: MD
authors.lastname: Lai
mesh_terms: D000818:Animals; D019556:COS Cells; D002413:Cations, Divalent / Q000378:metabolism / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003035:Cobalt / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D003067:Coenzymes; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D003035:Cobalt; D004492:Edetic Acid; D017346:Protein Serine-Threonine Kinases; D015032:Zinc