title: Structural analysis of a neutral (1-->3),(1-->4)-beta-D-glucan from the mycelia of Cordyceps sinensis.
abstract: The extracellular polysaccharide (1) extracted from the mycelia of Cordyceps sinensis with hot water was fractionated and purified by ion-exchange and gel-filtration chromatography. The structure was investigated using methylation and hydrolysis analysis, periodate oxidation, NMR spectroscopy, and reaction with beta-D-glucanase, and the results indicated that this D-glucan consisted of a backbone composed of (1-->3)-beta-D-glucosyl residues and carried a single (1-->4)-beta-linked D-glucosyl residue. NMR and IR spectroscopic measurements showed that the sugar residues were beta-glycosidically linked.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, 310027, People's Republic of China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Wu
authors.forename: Cuirong
authors.initials: C
authors.lastname: Sun
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D002681:China; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D032661:Cordyceps / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D025282:Mycelium / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
chemical_list: D005936:Glucans