[Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2].
OBJECTIVE
To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.


METHODS
Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.


RESULTS
PC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.


CONCLUSIONS
RNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.
Zhou
Li-quan
LQ
Institute of Biotechnology, Military Academy of Medical Science, Beijing 100850, China.
Zhang
Hui
H
Gao
Xue-song
XS
Wang
Jian
J
Liang
Rui-xia
RX
Hong
Bao-fa
BF
Zhou
Jian-guang
JG
D045744:Cell Line, Tumor; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011755:Pyrophosphatases / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D012333:RNA, Messenger; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases