Nonactin biosynthesis: the product of the resistance gene degrades nonactin stereospecifically to form homochiral nonactate dimers.
Heterologous expression of the tetranactin resistance gene nonR in E. coli gave a his6-tagged protein that catalyzed the stereoselective hydrolysis of the chiral macrotetrolide antibiotic nonactin into equivalent, chiral, inactive nonactate dimer species. No trimeric nonactate was produced in the reaction.
Cox
James E
JE
Department of Chemistry, The University of Montana, Missoula, Montana 59812-1656, USA.
Priestley
Nigel D
ND
D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D018942:Macrolides / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D018942:Macrolides; C089115:ORF6 protein, Bacteria; C050476:nonactin