Transfection efficiency comparison of oligonucleotide and plasmid to the HL-60 cell line with liposomes.
The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminePLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90% - 95% and it had no obvious attenuation within 84 h. However, the plasmid transfection rate was only 5% -25% and it was decreased significantly within 60 h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.
Tang
Yi
Y
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Wenli
W
Zhou
Jianfeng
J
Xu
Huizhen
H
Lu
Wu
W
D049452:Green Fluorescent Proteins / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D008081:Liposomes; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D014162:Transfection
D008081:Liposomes; D009841:Oligonucleotides; D049452:Green Fluorescent Proteins