title: Bone marker levels in gingival crevicular fluid during orthodontic intrusive tooth movement: a preliminary study.
abstract: The aim of the present study is to observe the changes in bone turnover markers, deoxypyridinoline (Dpd), osteocalcin, n-telopeptide (NTx), and bone alkaline phosphatase (balp) during the experimental orthodontic intrusion of maxillary premolar teeth. The study population required fixed appliance therapy involving the extraction of the maxillary first premolar teeth. Gingival crevicular fluid (GCF) samples were collected from each patient by using paper strips before the appliances were fitted and 1, 24, and 168 hours after the activation of appliances. After the second activation on the 21st, 22nd, and 28th days of the study, samples were collected. Enzyme-Linked Immunosorbent Assay (ELISA) tests were performed following manufacturer's recommendations. The results of the study indicate Dpd, osteocalcin, and balp values decrease with force application. Among the tested parameters only Dpd values showed statistically significant changes through time. One, 7, 22, and 28 day results show a significant amount of decrease when compared to 0 days. The extra decrease on the 22nd day (the day after the second activation) is also significantly lower. NTx crosslink values could not be detected in the experimental samples.
authors.affiliation: Department of Orthodontics, Faculty of Dentistry, Yeditepe University, Istanbul, Turkey. drfulya@veditepe.edu.tr
authors.forename: F
authors.initials: F
authors.lastname: Isik
authors.forename: K
authors.initials: K
authors.lastname: Sayinsu
authors.forename: T
authors.initials: T
authors.lastname: Arun
authors.forename: Y
authors.initials: Y
authors.lastname: Unlüçerçi
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000032:analysis; D000539:Alveolar Process / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D016723:Bone Remodeling / Q000502:physiology*; D016009:Chi-Square Distribution; D003094:Collagen / Q000032:analysis; D024042:Collagen Type I; D003799:Dental Stress Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D015675:Osteocalcin / Q000032:analysis; D010455:Peptides / Q000032:analysis; D018709:Statistics, Nonparametric; D014087:Tooth Movement Techniques
chemical_list: D000596:Amino Acids; D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; C036020:deoxypyridinoline; D003094:Collagen; D000469:Alkaline Phosphatase