title: Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary.
abstract: Fundulus heteroclitus (Atlantic killifish, mummichog) from a highly contaminated site on the Elizabeth River (VA, USA) are resistant to the toxicity of sediment from the site. However, the mechanistic changes that confer resistance to the toxicity are not yet well understood. We utilized differential display to identify mRNAs that are differentially expressed in hepatic tissue of male and female killifish from the Elizabeth River environment, compared to killifish from a non-contaminated reference site, King's Creek (VA, USA). Seventy-four differentially expressed mRNAs were initially identified (including sex and population-specific differences), and 65 of these were isolated and sequenced. A reverse northern blot array constructed of these cDNAs (plus an additional 15 previously sequenced mRNAs of interest) was used to confirm and quantify expression differences. High interindividual variability was observed in mRNA expression, but multiple differentially expressed mRNAs were identified, including 11 population-specific differences occurring in both sexes, 24 population-specific differences occurring in only one sex, and 22 sex-specific differences. Many of these differentially expressed mRNAs were novel, or not previously hypothesized to play a role in response to contaminant exposure. In addition, the results indicate that the effect of contaminated sediment exposure on the expression of a large proportion of the differentially expressed mRNAs was dependent on the sex of the fish.
authors.affiliation: Nicholas School of the Environment and Earth Sciences, and Integrated Toxicology Program, Duke University, Durham, NC 27708-0328, USA.
authors.forename: Joel N
authors.initials: JN
authors.lastname: Meyer
authors.forename: David C
authors.initials: DC
authors.lastname: Volz
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Freedman
authors.forename: Richard T
authors.initials: RT
authors.lastname: Di Giulio
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D017931:DNA Primers; D004351:Drug Resistance / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D023781:Fundulidae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D019015:Geologic Sediments / Q000032:analysis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012737:Sex Factors; D014768:Virginia
chemical_list: D017931:DNA Primers; D004785:Environmental Pollutants; D012333:RNA, Messenger