title: [Morphological and molecular biological peculiarities of the experimental autoimmune prostatitis rat model].
abstract: OBJECTIVE
  To observe the morphological and molecular biological peculiarities of the experimental autoimmune prostatitis (EAP) rat model made by SC purified prostate protein twice with immune adjuvant.
  
  
  METHODS
  Male rats were intradermally immunized with a saline extract of male rat prostate glands (RPG) in Freund's complete adjuvant (FCA) and Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. at the 0 and 30th day, and the concentrations of the extract were respectively 5 mg/ml, 10 mg/ml and 15 mg/ml. At the 45th day, the rats were sacrificed and the morphological and molecular biological changes of the prostate specimens were observed to determine the effective concentration of RPG for a successful model.
  
  
  RESULTS
  The expression of inflammation genes such as TNF-alpha, IL-1beta, IL-2 and iNOS obviously increased in the high-dosage model group; LM, EM and in situ hybridization revealed appearant chronic inflammation response, but this was not the case in the other two dosage groups.
  
  
  CONCLUSIONS
  15 mg/ml RPG mixed with FCA (1:1) 1.0 ml SC with Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. was an effective dosage for the successful model in our experiment.
authors.affiliation: Zhejiang University School of Pharmaceutical Sciences, Hangzhou, Zhejiang 310031, China. zhxh@zju.edu.cn
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Zhi-heng
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Zhong-de
authors.initials: ZD
authors.lastname: Liu
authors.forename: Ji-xiang
authors.initials: JX
authors.lastname: Yang
authors.forename: Yan-wei
authors.initials: YW
authors.lastname: Lv
authors.forename: Chun-lai
authors.initials: CL
authors.lastname: You
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000008:administration & dosage; D004195:Disease Models, Animal; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D008297:Male; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D011472:Prostatitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015721:Diphtheria-Tetanus-Pertussis Vaccine; D011506:Proteins; D005620:Freund's Adjuvant