Changes in erythrocyte enzymes in humans long-term exposed to pesticides: influence of several markers of individual susceptibility.
Changes in erythrocyte delta-aminolevulinic acid dehydratase (ALA-D) have been reported after exposure to different pesticides, including organophosphates and paraquat. In this study, we have determined ALA-D in 135 pesticide applicators (sprayers) from an intensive agriculture setting at two periods with different pesticide exposure. Acetylcholinesterase (AChE) was used as a reference biomarker. The effects of the combined polymorphism of enzymes involved in the detoxification of pesticides (paraoxonase (PON1), benzoylcholinesterase (BChE), and glutathione S-transferase (GSTM1 and GSTT1)) on the level of the target erythrocyte enzymes were also studied as biomarkers of individual susceptibility. Sprayers presented significant lower levels of ALA-D and AChE than controls (41.3% and 14.5%, respectively) at the high exposure period. When all biomarkers of individual susceptibility to pesticides were considered at the same time, the GSTT1 null allele determined higher ALA-D and AChE activities at the period of high exposure to pesticides. PON1 R allele in turn determined lower AChE activity at the low exposure period. Null genotype for both GST subclasses (GSTM1 and GSTT1) was found to be the unique independent predictor of pesticide-related symptomatology. Interestingly, sprayers were consistently underrepresented among carriers of "unfavourable" BChE variants. In conclusion, ALA-D appears to be an important biological indicator of pesticide exposure and PON1 and GSTT1 are relevant determinants of susceptibility to chronic pesticide poisoning.
Hernández
Antonio F
AF
Department of Legal Medicine and Toxicology, University of Granada School of Medicine, Granada, Spain. ajerez@ugr.es
López
Olga
O
Rodrigo
Lourdes
L
Gil
Fernando
F
Pena
Gloria
G
Serrano
José Luis
JL
Parrón
Tesifón
T
Alvarez
Juan Carlos
JC
Lorente
José Antonio
JA
Pla
Antonio
A
D000109:Acetylcholine / Q000097:blood; D000293:Adolescent; D000328:Adult; D000383:Agriculture; D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics; D015415:Biomarkers; D002091:Butyrylcholinesterase / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D010575:Pesticides / Q000633:toxicity*; D011110:Polymorphism, Genetic; D000623:Porphobilinogen Synthase / Q000097:blood / Q000235:genetics
D015415:Biomarkers; D010575:Pesticides; D005979:Glutathione Peroxidase; D002091:Butyrylcholinesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D000623:Porphobilinogen Synthase; D000109:Acetylcholine