[Effects of pentachlorophenol on rat sertoli cells].
OBJECTIVE
To detect the toxic effects of pentachlorophenol (PCP) on cultured rat Sertoli cells.


METHODS
The viability of Sertoli cells was detected and morphological examination was performed, followed by flow cytometric assay to evaluate the toxic effect of PCP on rat Sertoli cells.


RESULTS
MTT assay showed that PCP induced a concentration- and time-dependent decrease in Sertoli cell viability. Flow cytometric assay revealed that the number of dead Sertoli cells grew along with increased exposure to PCP.


CONCLUSIONS
PCP, with obvious cytotoxic effects, can cause necrosis of Sertoli cells in vitro.
Yang
Shu-zhen
SZ
Laboratory of Immunology and Reproductive Biology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
Han
Xiao-dong
XD
Chen
Wei
W
Yin
Da-qiang
DQ
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008297:Male; D010416:Pentachlorophenol / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000502:physiology
D010416:Pentachlorophenol