Solution stirring initiates nucleation and improves the quality of adenosine deaminase crystals.
Crystals of bovine adenosine deaminase (ADA) grown over a two-week period in the presence of an inhibitor (ADA complex) were found to be of low quality for X-ray diffraction analysis. Furthermore, ADA incubated in the absence of an inhibitor (ADA native) did not form any crystals using conventional crystallization methods. A solution-stirring technique was used to obtain high-quality ADA complex and ADA native crystals. The crystals obtained using this technique were found to be of high quality and were shown to have high structural resolution for X-ray diffraction analyses. The results reported here indicate that the solution-stirring technique promotes nucleation and improves the quality of protein crystals.
Adachi
Hiroaki
H
Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. adachi@pwr.eng.osaka-u.ac.jp
Takano
Kazufumi
K
Niino
Ai
A
Matsumura
Hiroyoshi
H
Kinoshita
Takayoshi
T
Warizaya
Masaichi
M
Inoue
Tsuyoshi
T
Mori
Yusuke
Y
Sasaki
Takatomo
T
D000243:Adenosine Deaminase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D012996:Solutions / Q000737:chemistry
D012996:Solutions; D000243:Adenosine Deaminase