Human CC chemokine CCL23, a ligand for CCR1, induces endothelial cell migration and promotes angiogenesis.
A number of chemokines induce angiogenesis and endothelial cells express several chemokine receptors. To date, only a limited number of CC chemokines for CCR1 have been reported to induce angiogenic responses. We investigated the ability of CCL23 (also known as MPIF-1, MIP-3, or CKbeta8) to promote angiogenesis, which induces chemotaxis of immune cells through CCR1. CCL23 promoted the chemotactic migration and differentiation of endothelial cells, and neovascularization in the chick chorioallantoic membrane. An N-terminal truncated form of CCL23 was at least 100-fold more potent than its intact form and was comparable to that of FGF in the angiogenic activities. Treatment with either pertussis toxin or anti-CCR1 antibody completely inhibited the CCL23-induced endothelial cell migration, indicating that endothelial cell migration was mediated through CCR1. CCL23 didn't promote the migration of HT1080 human fibrosarcoma cells that did not express CCR1. Our results suggest a role of CCL23 in angiogenesis in vitro as well as in vivo.
Hwang
Jungsu
J
Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin 449-701, Republic of Korea.
Son
Kyung-No
KN
Kim
Chan Woo
CW
Ko
Jesang
J
Na
Doe Sun
DS
Kwon
Byoung S
BS
Gho
Yong Song
YS
Kim
Jiyoung
J
D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000378:metabolism / Q000494:pharmacology*; D002642:Chick Embryo; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology; D054389:Receptors, CCR1; D019707:Receptors, Chemokine / Q000378:metabolism*; D014470:Umbilical Cord / Q000098:blood supply / Q000166:cytology / Q000378:metabolism
C105504:CCL23 protein, human; C515300:CCR1 protein, human; D019742:Chemokines, CC; D008024:Ligands; D010446:Peptide Fragments; D054389:Receptors, CCR1; D019707:Receptors, Chemokine