title: [Effect of eucommia ulmoides oliv on SOD activity and alpha-actin expression in the penile tissues of diabetic rats].
abstract: OBJECTIVE
  To study the effect of aqueous abstract from eucommia ulmoides oliv on the activity of superoxide dismutase (SOD) and alpha-actin expression in the penile tissues of rats with diabetes mellitus (DM) in vitro.
  
  
  METHODS
  A diabetes model was established by administration of alloxun twice to Sprague Dawley (SD) rats. Ten diabetic and 10 normal rats were randomly selected and the penile strips of each rat were divided into four equal shares and cultured in two groups, a eucommia ulmoides oliv coculture group (Group A, further dicided into 1 microg/ml, 10 microg/ml and 100 microg/ml subgroups) and a control group (Group B). Seven days later, the activity of SOD in the culture medium was detected by spectrophotometry, and the levels of micro-actin expression in the penile tissues were examined with the immunohistochemical method.
  
  
  RESULTS
  Compared with Group B, the activity of SOD in the culture medium in athe 10 and 100 microg/ml subgroups was notably elevated (P < 0.01), and the numbers of immunoreactive positive cells of alpha-actin in the penile tissues remarkably increased (P < 0.01).
  
  
  CONCLUSIONS
  The activity of SOD and alpha-actin expression in the penile tissues of diabetic rats in vitro can be increased by eucommia ulmioides oliv.
authors.affiliation: Department of Venereology, Wuhan Institute of Dermatology and Venereology, Wuhan, Hubei 430031, China. whzwh1718@hotmail.com
authors.forename: Wan-hong
authors.initials: WH
authors.lastname: Zhang
authors.forename: Zi-long
authors.initials: ZL
authors.lastname: Liu
authors.forename: Chao-dong
authors.initials: CD
authors.lastname: Liu
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Zhu
authors.forename: Cheng-liang
authors.initials: CL
authors.lastname: Xiong
authors.forename: Han-sheng
authors.initials: HS
authors.lastname: Dong
mesh_terms: D000199:Actins / Q000096:biosynthesis*; D000496:Alloxan; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D031283:Eucommiaceae; D008297:Male; D010413:Penis / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
chemical_list: D000199:Actins; D010936:Plant Extracts; D000496:Alloxan; D013482:Superoxide Dismutase