Rotary culture enhances pre-osteoblast aggregation and mineralization.
Three-dimensional environments have been shown to enhance cell aggregation and osteoblast differentiation. Thus, we hypothesized that three-dimensional (3D) growth environments would enhance the mineralization rate of human embryonic palatal mesenchymal (HEPM) pre-osteoblasts. The objective of this study was to investigate the potential use of rotary cell culture systems (RCCS) as a means to enhance the osteogenic potential of pre-osteoblast cells. HEPM cells were cultured in a RCCS to create 3D enviroments. Tissue culture plastic (2D) cultures served as our control. 3D environments promoted three-dimensional aggregate formations. Increased calcium and phosphorus deposition was significantly enhanced three- to 18-fold (P < 0.001) in 3D cultures as compared with 2D environments. 3D cultures mineralized in 1 wk as compared with the 2D cultures, which took 4 wks, a decrease in time of nearly 75%. In conclusion, our studies demonstrated that 3D environments enhanced osteoblast cell aggregation and mineralization.
Facer
S R
SR
Department of Endodontics, University of Iowa, College of Dentistry, IA 52242, USA.
Zaharias
R S
RS
Andracki
M E
ME
Lafoon
J
J
Hunter
S K
SK
Schneider
G B
GB
D002113:Calcification, Physiologic / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D004396:Coloring Agents; D004577:Electron Probe Microanalysis; D006801:Humans; D008648:Mesoderm / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010159:Palate / Q000196:embryology; D010758:Phosphorus / Q000378:metabolism; D012399:Rotation; D013997:Time Factors
D004396:Coloring Agents; D010758:Phosphorus; D002118:Calcium