Evidence for the presence of an alternative glucose transport system in Clostridium beijerinckii NCIMB 8052 and the solvent-hyperproducing mutant BA101.
The effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by Clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a non-PTS system probably energized by the transmembrane proton gradient. In both wild-type C. beijerinckii NCIMB 8052 and the butanol-hyperproducing mutant BA101, PTS activity declined at the end of exponential growth, while glucokinase activity increased in the later stages of fermentation. The non-PTS uptake system, together with enhanced glucokinase activity, may provide an explanation for the ability of the mutant to utilize glucose more effectively during fermentation despite the fact that it is partially defective in PTS activity.
Lee
Jieun
J
Food Microbiology Division, Department of Food Science and Human Nutrition, University of Illinois, Urbana, Illinois 61801, USA.
Mitchell
Wilfrid J
WJ
Tangney
Martin
M
Blaschek
H P
HP
D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005941:Glucokinase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D010766:Phosphorylation; D012997:Solvents / Q000378:metabolism*
D000440:Butanols; D012997:Solvents; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005941:Glucokinase; D005947:Glucose