Role of Staphylococcus aureus global regulators sae and sigmaB in virulence gene expression during device-related infection.
The ability of Staphylococcus aureus to adapt to different environments is due to a regulatory network comprising several loci. Here we present a detailed study of the interaction between the two global regulators sae and sigmaB of S. aureus and their influence on virulence gene expression in vitro, as well as during device-related infection. The expression of sae, asp23, hla, clfA, coa, and fnbA was determined in strain Newman and its isogenic saeS/R and sigB mutants by Northern analysis and LightCycler reverse transcription-PCR. There was no indication of direct cross talk between the two regulators. sae had a dominant effect on target gene expression during device-related infection. SigmaB seemed to be less active throughout the infection than under induced conditions in vitro.
Goerke
Christiane
C
Institut für Med. Mikrobiologie und Hygiene, Universitätsklinikum Tübingen, Wilhelmstrasse 31, D-72074 Tübingen, Germany. christiane.goerke@uni-tuebingen.de
Fluckiger
Ursula
U
Steinhuber
Andrea
A
Bisanzio
Vittoria
V
Ulrich
Martina
M
Bischoff
Markus
M
Patti
Joseph M
JM
Wolz
Christiane
C
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D019736:Prostheses and Implants / Q000009:adverse effects*; D012333:RNA, Messenger / Q000032:analysis; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014157:Transcription Factors; D014774:Virulence
D001426:Bacterial Proteins; D012333:RNA, Messenger; C433805:SaeR protein, Staphylococcus aureus; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D014157:Transcription Factors