ATR-IR spectroscopy as applied to nucleic acid films.
For the first time the ATR technique was applied to obtain IR absorption spectra of DNA and RNA dry films. There was worked out procedure of the nucleic acid removal from germanium plate, which obviously was a main obstacle to application of ATR-IR spectroscopy to nucleic acids. This technique of IR spectroscopy was applied to confirmation of RNA tropism of aurin tricarboxylic acid observed by molecular biological methods.
Stepanyugin
Andriy V
AV
Institute of Molecular Biology and Genetics, Ukrainian National Academy of Sciences, 150 vul. Zabolotnoho, 03143 Kyiv, Ukraine.
Samijlenko
Svitlana P
SP
Martynenko
Olena I
OI
Hovorun
Dmytro M
DM
D010582:Bacteriophage lambda; D004247:DNA / Q000737:chemistry*; D005857:Germanium / Q000737:chemistry; D031962:Kalanchoe; D012313:RNA / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D005857:Germanium; D012313:RNA; D004247:DNA