title: Molecular profiling demonstrates limited diversity amongst geographically separate strains of Ustilago scitaminea.
abstract: Intraspecies diversity within Ustilago scitaminea isolates from South Africa, Reunion Island, Hawaii and Guadeloupe was assessed by RAPDs, bE mating-type gene detection, rDNA sequence analysis, microscopy and germination and morphological studies. Except for sequence data, the other analyses yielded no differences in the isolates that could be used in a phylogenetic separation. Mycelial DNA of the SA isolate shared 100% sequence identity with that of mycelial DNA cultured from in vitro produced teliospores of the parent cultivar. Overall the ITS1 and ITS2 regions were found to have 96.1% and 96.9% sequence identity with a total of 17 and 21 base changes, respectively, amongst the isolates. The Reunion Island isolate was shown to be most distantly related by 3.6% to the other isolates, indicating a single clonal lineage. The lack of germination in teliospores from Guadeloupe may be attributed to changes in temperature and humidity during transportation.
authors.affiliation: Department of Botany, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Durban, South Africa. singhni@ukzn.ac.za
authors.forename: Nisha
authors.initials: N
authors.lastname: Singh
authors.forename: Benesh M
authors.initials: BM
authors.lastname: Somai
authors.forename: Dorsamy
authors.initials: D
authors.lastname: Pillay
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D014644:Genetic Variation; D019430:Guadeloupe; D006254:Hawaii; D008969:Molecular Sequence Data; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D013019:South Africa; D014588:Ustilago / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer