Development of a genetic traceability test in pig based on single nucleotide polymorphism detection.
In order to assure traceability along the meat transformation process, a powerful system is required. The administrative traceability shows limits that the use of genetic markers could overcome. The individual genomes contain sequence differences, basis of the genetic polymorphism of which the genetic markers are the witnesses. Among them, two classes seem to dominate on the traceability field: the microsatellites and the single nucleotide polymorphisms (SNP). The aim of this work was to develop a genetic traceability test in pig based on SNPs mainly located in 5' and 3' untranslated regions (UTRs). A set of 21 SNP markers including new SNPs identified in this study and SNPs previously described was selected. A genotyping assay was performed on 96 individuals representing the major crossbred of the pig population in Belgium. Results showed that all individuals tested presented a different genotype. This genotyping method might help the administrative system to guarantee the traceability of pork meat along the transformation process.
Goffaux
F
F
Department of Food Sciences, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, 20/B43bis, 4000 Liege, Belgium.
China
B
B
Dams
L
L
Clinquart
A
A
Daube
G
G
D000823:Animal Identification Systems / Q000379:methods*; D000818:Animals; D003257:Consumer Product Safety; D004247:DNA / Q000032:analysis*; D005511:Food Handling; D005819:Genetic Markers; D005838:Genotype; D008460:Meat / Q000592:standards; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA