Nerve-pathways of acupoint Fengch'ih in rat by anterograde transport of HRP.
OBJECTIVE
To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP).


METHODS
Fifty Wistar rats were randomly divided into 1, 2, 3, 4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih. Serial, transverse or capital, 40 microm sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red.


RESULTS
After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased.


CONCLUSIONS
Fengch'ih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.
Xi
Gang-Ming
GM
Department of Neurology, Shiyan Renmin Hospital Affiliated to Yunyang Medical College, Shiyan, Hubei Province, China.
Wang
Hua-Qiao
HQ
He
Guo-Hou
GH
Huang
Chao-Feng
CF
Yuan
Qun-Fang
QF
Wei
Guo-Yao
GY
Li
Hua
H
Liu
Wen-Wen
WW
Fan
Hua-Yan
HY
D015669:Acupuncture Points; D000818:Animals; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D009434:Neural Pathways / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions
D012996:Solutions; D006735:Horseradish Peroxidase