title: Spectrophotometric determination of DNA using a near infrared probe 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine.
abstract: A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris-HCl buffer, the reaction of DSTCY with DNA was complete within 10 min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5-8.0 microg ml(-1) and the detection limit of 45 ng ml(-1).
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Li
authors.forename: Yin
authors.initials: Y
authors.lastname: Lu
authors.forename: Ni-Na
authors.initials: NN
authors.lastname: Fu
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D002021:Buffers; D002232:Carbocyanines / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared; D013696:Temperature
chemical_list: C479728:1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine; D002021:Buffers; D002232:Carbocyanines; D002593:Cetrimonium Compounds; D004247:DNA; C427203:calf thymus DNA; D000077286:Cetrimonium