title: Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting.
abstract: Yeast population used in industrial production of fuel-ethanol may vary according to the plant process condition and to the environmental stresses imposed to yeast cells. Therefore, yeast strains isolated from a particular industrial process may be adapted to such conditions and should be used as starter strain instead of less adapted commercial strains. This work reports the use of PCR-fingerprinting method based on microsatellite primer (GTG)5 to characterize the yeast population dynamics along the fermentation period in six distilleries. The results show that indigenous fermenting strains present in the crude substrate can be more adapted to the industrial process than commercial strains. We also identified new strains that dominate the yeast population and were more present either in molasses or sugar cane fermenting distilleries. Those strains were proposed to be used as starters in those industrial processes. This is the first report on the use of molecular markers to discriminate Saccharomyces cerevisiae strains from fuel-ethanol producing process.
authors.affiliation: Setor de Biologia Molecular-LIKA, Brazil.
authors.forename: Eurípedes Alves
authors.initials: EA
authors.lastname: da Silva-Filho
authors.forename: Scheila Karina
authors.initials: SK
authors.lastname: Brito dos Santos
authors.forename: Alecsandra do Monte
authors.initials: Ado M
authors.lastname: Resende
authors.forename: José Otamar Falcão
authors.initials: JO
authors.lastname: de Morais
authors.forename: Marcos Antonio
authors.initials: MA
authors.lastname: de Morais
authors.forename: Diogo
authors.initials: D
authors.lastname: Ardaillon Simões
mesh_terms: D015415:Biomarkers; D001938:Brazil; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D018895:Microsatellite Repeats; D008965:Molasses; D010449:Peptide Mapping; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000502:physiology; D031786:Saccharum / Q000737:chemistry
chemical_list: D015415:Biomarkers; D000431:Ethanol