A putative novel alpha/beta hydrolase ORFan family in Bacillus.
A large number of sequences in each newly sequenced genome correspond to lineage and species-specific proteins, also known as ORFans. Amongst these ORFans, a large number are sequences with unknown structures and functions. We have identified a family of sequences, annotated as hypothetical proteins, which are specific to Bacillus and have carried out a computational study aimed at characterizing this family. Fold-recognition methods predict that these sequences belong to the alpha/beta hydrolase fold. We suggest possible catalytic triads for the ORFans and propose a hypothesis regarding the possible families within the alpha/beta hydrolase superfamily to which they may belong.
Siew
Naomi
N
Department of Chemistry, Ben Gurion University, Beer-Sheva 84105, Israel.
Saini
Harpreet Kaur
HK
Fischer
Daniel
D
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D002384:Catalysis; D019295:Computational Biology; D006867:Hydrolases / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013045:Species Specificity; D013329:Structure-Activity Relationship
D006867:Hydrolases