Inhibitory effects of acylated kaempferol glycosides from the leaves of Cinnamomum kotoense on the proliferation of human peripheral blood mononuclear cells.
A new chemical entity, namely kaempferol 3- O-alpha-L-[2-(Z)-p-coumaroyl-4-(E)-p-coumaroyl]rhamnopyranoside (1), and the known kaempferol 3-O-alpha-L-[2,4-di-(E)-p-coumaroyl]rhamnopyranoside (2) have been isolated from the methanolic extract of leaves of Cinnamomum kotoense. Structural elucidation of compounds 1 and 2 were achieved on the basis of spectroscopic analysis. The effects of compounds 1 and 2 on phytohemagglutinin (PHA) stimulated cell proliferation were studied towards human peripheral blood mononuclear cells (PBMC). The results indicated that compounds 1 and 2 suppressed PBMC proliferation induced by PHA with an IC50 value of 5.0 +/- 1.3 and 6.0 +/- 1.5 microM, respectively. Interleukin-2 production in activated PBMC inhibited by compounds 1 and 2 were in a concentration-dependent manner. Therefore, we suggested that compounds 1 and 2 in C. kotoense were likely the growth modulators for PBMC.
Kuo
Yuh-Chi
YC
Department of Life Science, Fu-Jen Catholic University, Taipei, Taiwan.
Lu
Chung-Kung
CK
Huang
Li-Wei
LW
Kuo
Yueh-Hsiung
YH
Chang
Chen
C
Hsu
Feng-Lin
FL
Lee
Tzong-Huei
TH
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D049109:Cell Proliferation / Q000187:drug effects; D027424:Cinnamomum; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D044949:Kaempferols / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006027:Glycosides; D044949:Kaempferols; D010936:Plant Extracts; C006552:kaempferol