Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors.
Prominin-1/CD133 is a plasma membrane marker found in several types of somatic stem cells, including hematopoietic and neural stem cells. To study its role during development and with differentiation, we analyzed its temporal and spatial expression (mRNA and protein) in preimplantation embryos, undifferentiated mouse embryonic stem (ES) cells, and differentiated ES cell progeny. In early embryos, prominin-1 was expressed in trophoblast but not in cells of the inner cell mass; however, prominin-1 transcripts were detected in undifferentiated ES cells. Both ES-derived cells committed to differentiation and early progenitor cells coexpressed prominin-1 with early lineage markers, including the cytoskeletal markers (nestin, cytokeratin 18, desmin), fibulin-1, and valosin-containing protein. After spontaneous differentiation at terminal stages, prominin-1 expression was downregulated and no coexpression with markers characteristic for neuroectodermal, mesodermal, and endodermal cells was found. Upon induction of neuronal differentiation, some prominin-1-positive cells, which coexpressed nestin and showed the typical morphology of neural progenitor cells, persisted until terminal stages of differentiation. However, no coexpression of prominin-1 with markers of differentiated neural cells was detected. In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells.
Kania
Gabriela
G
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany.
Corbeil
Denis
D
Fuchs
Joerg
J
Tarasov
Kirill V
KV
Blyszczuk
Przemyslaw
P
Huttner
Wieland B
WB
Boheler
Kenneth R
KR
Wobus
Anna M
AM
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD; D001755:Blastocyst / Q000166:cytology; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D004396:Coloring Agents / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D006023:Glycoproteins / Q000096:biosynthesis*; D018398:Homeodomain Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D000071317:Nanog Homeobox Protein; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000378:metabolism; D010455:Peptides; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors
D000071916:AC133 Antigen; D015703:Antigens, CD; D004396:Coloring Agents; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D010455:Peptides; C000606024:Prom1 protein, mouse; D012333:RNA, Messenger; D001973:Bromodeoxyuridine