title: Development of miniaturized multi-channel high-performance liquid chromatography for high-throughput analysis.
abstract: We have developed miniaturized multi-channel high-performance liquid chromatography (HPLC) system. With this system, we can simultaneously separate multiple samples, using a single high-pressure gradient pump, a chip-based sample injection unit, a monolithic silica capillary column array, and a multi-channel UV detection unit based on fiber optics. The injection unit has a simplified structure composed of brass housing and a quartz microchip having microchannels and access ports, which enable a direct injection of sample to multi-channel by commercial multichannel micropipette. Moreover, that possesses a function of microvalve, and on-chip definition of sample injection plugs achieved with a cross channel injection method, providing each column of monolithic silica capillary array. The substances in channels were simultaneously detected with UV having multiple cells. Standard samples were analyzed for characterizing newly developed system, and sharp peaks were obtained with reproducibility data of < 0.9% (R.S.D.). Analysis of tryptic digestion of casein was also employed. These results show that the novel multi-channel HPLC system has the benefits for the high-throughput analysis in the post-genomic analysis/combinatorial chemistry.
authors.affiliation: GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan. shintani@gls.co.jp
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shintani
authors.forename: Keiji
authors.initials: K
authors.lastname: Hirako
authors.forename: Masanori
authors.initials: M
authors.lastname: Motokawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Iwano
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhou
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Takano
authors.forename: Masahiro
authors.initials: M
authors.lastname: Furuno
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Minakuchi
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D008904:Miniaturization; D010455:Peptides / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010455:Peptides