Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells.
The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.
Tirado
Rocio
R
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Cd. Universitaria, Mexico D.F. 04510, Mexico.
Ortega
Arturo
A
Sarmiento
Rosa Elena
RE
Gómez
Beatríz
B
D000954:Antigens, Surface / Q000032:analysis / Q000502:physiology; D000956:Antigens, Viral / Q000032:analysis; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D005590:Fractional Precipitation; D015870:Gene Expression / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D047468:Immunoprecipitation; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018113:Respiratory Syncytial Virus, Human / Q000378:metabolism* / Q000528:radiation effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014357:Trypsin / Q000378:metabolism; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects; D014759:Viral Envelope Proteins / Q000378:metabolism / Q000494:pharmacology; D014760:Viral Fusion Proteins / Q000378:metabolism / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
D000954:Antigens, Surface; D000956:Antigens, Viral; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C116204:attachment protein G; D011092:Polyethylene Glycols; D014357:Trypsin