Genetic basis for conversion of rough-to-smooth colony morphology in Actinobacillus actinomycetemcomitans.
The basis of the rough-to-smooth conversion of Actinobacillus actinomycetemcomitans was examined. Smooth variants often contained mutations at the flp promoter region. Replacing the mutated flp promoter with the wild-type promoter restored the rough phenotype. The expression level of the flp promoter was approximately 100-fold lower in smooth than in rough strains. Mutations of the flp promoter are a cause of the rough-to-smooth conversion.
Wang
Ying
Y
Division of Primary Oral Health Care, University of Southern California School of Dentistry, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.
Liu
Amy
A
Chen
Casey
C
D016976:Aggregatibacter actinomycetemcomitans / Q000166:cytology* / Q000235:genetics* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D001426:Bacterial Proteins; C113731:Flp protein, bacteria