Propulsive activity induced by sequential electrical stimulation in the descending colon of the pig.
This work was performed to study electrically induced contractions in the descending colon of pigs. Contractions were monitored using impedance planimetry and manometry. The luminal pressure, cross-sectional area (CSA), latency and velocity of CSA decrease were compared when using 3 ms, 9, 12, 15 or 30 mA pulses at 10 Hz for 10 s, and 15 mA, 0.03, 0.3 or 3 ms pulses at 10 Hz for 10 s. Stimulation was performed prior and after the application of N(G)-nitro-L-arginine methyl ester (L-NAME) and atropine. In the untreated colon, contraction was always of an 'off' type. A current increase from 9 to 30 mA increased the pressure. An increase of pulse duration from 0.03 to 3 ms shortened the latency, accelerated contraction and increased pressure. By sequential stimulation, contractions were coordinated to propel semi-fluid and solid luminal contents. L-NAME increased the magnitude of CSA decrease. Atropine induced inhibitory effects on contractions elicited by 3 ms pulses and abolished contractions induced by 0.03 and 0.3 ms pulses.


CONCLUSIONS
(i) electrical stimulation evokes'off' colon contractions, which can be coordinated to result in propulsion; (ii) the best combination for current and pulse duration to induce propulsive contractions is 15 mA and 3 ms; (iii) nitrergic and cholinergic pathways mediate responses to electrical stimulation.
Sevcencu
C
C
Center for Sensory-Motor Interaction (SMI), Department of Health Science and Technology, Aalborg University, Denmark. sevcr@smi.auc.dk
Rijkhoff
N J M
NJ
Gregersen
H
H
Sinkjaer
T
T
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003106:Colon / Q000294:innervation / Q000502:physiology*; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology; D010275:Parasympathetic Nervous System / Q000502:physiology; D010276:Parasympatholytics / Q000494:pharmacology; D013552:Swine
D004791:Enzyme Inhibitors; D010276:Parasympatholytics; D009569:Nitric Oxide; D001285:Atropine; D019331:NG-Nitroarginine Methyl Ester