Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated Douglas-Firby novel and commercial fungal cellulases.
Softwood residues are the most abundant feedstock available for bioconversion in many northern countries. However, the high costs for delignification and enzymatic hydrolysis currently deter commercialization of softwood bioconversion processes. This study evaluates the abilities of two novel fungal preparations (MSUBC1 and MSUBC2) and two commercial cellulase preparations (TR1 and TR2) to hydrolyze cellulose in Douglas-firpretreated by steam explosion or ethanol organosolv process. MSUBC1 showed significantly better performance than the other preparations on both lignocellulosic substrates. In particular, MSUBC1 achieved >76% cellulose conversion for hydrolysis of steam-exploded Douglas-fir (approximately 44% lignin) after 72 h at low enzyme loading (10 filter paper units/g of cellulose) and without beta-glucosidase supplementation.
Kurabi
Arwa
A
Forest Products Biotechnology, Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.
Berlin
Alex
A
Gilkes
Neil
N
Kilburn
Douglas
D
Bura
Renata
R
Robinson
Jamie
J
Markov
Aleksandr
A
Skomarovsky
Anton
A
Gusakov
Aleksandr
A
Okunev
Oleg
O
Sinitsyn
Arkady
A
Gregg
David
D
Xie
Dan
D
Saddler
John
J
D001673:Biodegradation, Environmental; D044602:Cellulases / Q000737:chemistry*; D004789:Enzyme Activation; D000431:Ethanol / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D010407:Penicillium / Q000201:enzymology*; D028224:Pseudotsuga / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology*; D014867:Water / Q000737:chemistry*; D014934:Wood
D014867:Water; D000431:Ethanol; D008031:Lignin; D044602:Cellulases