[Changes of subtype T lymphocytes in blood after implanting with xenogeneic acellular bone matrix].
OBJECTIVE
To observe the changes of immune status in recipient after implanting with xenogeneic acellular bone matrix (ACBM).


METHODS
Twenty rabbits were randomly divided into 4 groups. Autograft, ACBM and bone soaked in alcohol were implanted into the 3 experimental groups separately, and No-treatment was done as control group. The CD4+, CD8+, CD25+ T lymphocytes in blood were detected by flow cytometer at 1, 2, 4 and 6 weeks after operation. After 2 and 6 weeks of implantation, the changes of bone and tissue were observed by histology.


RESULTS
After 2-6 weeks, CD4+ and CD8+ T cells were significantly higher in the implanted group of bone soaked in alcohol than that in the other 3 groups (P < 0.05) and there was no statistically significant difference in the other 3 groups (P > 0.05). After 2 weeks, CD25+ T cells were significantly higher in the implanted group of bone soaked in alcohol than that in the other groups. In the 2nd week, there were inflammatory infiltration with a predominance of granulocytes. In the 6th week, there were many fibroblasts instead of granulocytes with a few lymphocytes and cartilage island formed in the implanted groups of autograft and ACBM.


CONCLUSIONS
ACBM implanting has low influence on cellular immunity in recipient.
Sun
Xinjun
X
Daping Hospital, Institute of Field Surgery, Chongqing, 400042, PR China.
Wang
Zhengguo
Z
Zhu
Peifang
P
D000818:Animals; D001857:Bone Matrix / Q000276:immunology / Q000637:transplantation*; D016025:Bone Transplantation / Q000276:immunology / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013997:Time Factors; D014183:Transplantation, Heterologous
D053645:Interleukin-2 Receptor alpha Subunit