Dielectric monitoring of growth and sporulation of Bacillus thuringiensis.
On-line permittivity and optical density measurements have been used to monitor biomass concentration and sporulation status during growth of a spore-forming bacterium, Bacillus thuringiensis, in fed-batch culture. The correlation between permittivity, optical density and other observations showed three distinct phases of growth: growth itself, transition and sporulation. The permittivity variations during the transition and sporulation phases could be related to the sporulation development: the evolution pattern of the ratio of optical density to permittivity was representative of the culture state, and during the sporulation phase, a permittivity index could be build to measure the extend of spore liberation.
Sarrafzadeh
M H
MH
UMR Ingénierie de la Réaction Biologique--Bioproductions, Université Montpellier II, 34095, Montpellier, France.
Belloy
L
L
Esteban
G
G
Navarro
J M
JM
Ghommidh
C
C
D000042:Absorption; D001413:Bacillus thuringiensis / Q000254:growth & development*; D004553:Electric Conductivity; D008954:Models, Biological; D013171:Spores, Bacterial / Q000502:physiology*