Cloning and characterization of a gene encoding a malic enzyme involved in anaerobic growth in Mucor circinelloides.
A 3193 bp contiguous sequence has been cloned from the oleaginous fungus Mucor circinelloides, that contains a full-length gene encoding a putative NADP+: dependent malic enzyme (EC. 1.1.1.40). The cloned DNA contains a 2154 bp putative open reading frame containing five introns and encoding a protein of 616 amino acids. The gene encoded what appeared to be an anaerobic isoform of malic enzyme (isoform II); this conclusion is supported by transcript analysis and by the fact that the ORF contains an N-terminal mitochondrial target sequence (a similar cellular location was identified for the anaerobic malic enzyme in Saccharomyces cerevisiae; Boles et al. 1998). The cloned gene did not encode either isoform III (the isoform associated with active growth) or isoform IV (associated with lipid accumulation) previously identified. M. circinelloides therefore must possess (at least) two structural genes for malic enzyme.
Li
Yonghua
Y
Centre for Lipid Research, University of Hull, Cottingham Road, Hull HU6 7RX, UK.
Adams
Ian P
IP
Wynn
James P
JP
Ratledge
Colin
C
D000693:Anaerobiosis; D003001:Cloning, Molecular; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009089:Mucor / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016366:Open Reading Frames
D007527:Isoenzymes; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)