Characterization of the C2 subdomain of yeast mitochondrial initiation factor 2.
The COOH-terminal part of the yeast mitochondrial initiation factor 2 (ymIF2), containing the C2 subdomain, was expressed and purified as a histidine-tagged polypeptide of 137 amino acids. Like the recombinant full-length protein, the C2 subdomain binds both formyl-Met-tRNA(f)(Met) and unformylated Met-tRNA(f)(Met) with only a small preference for the former species. Formation of a binary complex between the C2 subdomain or the full-length ymIF2 and initiator tRNA was also assessed by fluorescence measurements. The binding of coumarin-Met-tRNA(f) to either protein caused a blue shift of the coumarin emission spectrum and an increase in anisotropy. Full-length ymIF2 is functionally competent in forming an initiation complex and supporting formation of the first peptide bond on Escherichia coli ribosomes. The results demonstrate that ymIF2 has the same domain structure and biochemical properties of a typical IF2 species as found in bacteria or mammalian mitochondria--but with enhanced ability to bind unformylated initiator Met-tRNA.
Garofalo
Cristiana
C
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA.
Kramer
Gisela
G
Appling
Dean R
DR
D003374:Coumarins / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D008928:Mitochondria / Q000201:enzymology*; D010448:Peptide Initiation Factors / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D012358:RNA, Transfer, Met / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D003374:Coumarins; D010448:Peptide Initiation Factors; D012358:RNA, Transfer, Met; D029701:Saccharomyces cerevisiae Proteins; C030123:coumarin