Simultaneous biocatalyst production and Baeyer-Villiger oxidation for bioconversion of cyclohexanone by recombinant Escherichia coli expressing cyclohexanone monooxygenase.
Cyclohexanone monooxygenase (CHMO) catalyzing Baeyer-Villiger oxidation converts cyclic ketones into optically pure lactones, which have been used as building blocks in organic synthesis. A recombinant Escherichia coli BL21(DE3)/pMM4 expressing CHMO originated from Acinetobacter sp. NCIB 9871 was used to produce epsilon-caprolactone through a simultaneous biocatalyst production and Baeyer-Villiger oxidation (SPO) process. A fed-batch process was designed to obtain high cell density for improving production of epsilon-caprolactone. The fed-batch SPO process gave the best results, 10.2 g/L of epsilon-caprolactone and 0.34 g/(L.h) of productivity, corresponding to a 10.5- and 3.4-fold enhancement compared with those of the batch SPO, respectively.
Lee
Won-Heong
WH
School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Korea.
Park
Yong-Cheol
YC
Lee
Dae-Hee
DH
Park
Kyungmoon
K
Seo
Jin-Ho
JH
D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D002208:Caproates / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003512:Cyclohexanones / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
D002208:Caproates; D003512:Cyclohexanones; D007783:Lactones; D011994:Recombinant Proteins; C121056:caprolactone; C036468:cyclohexanone; D010105:Oxygenases; C019961:cyclohexanone oxygenase