title: Identification of leukemia-associated antigens in chronic myeloid leukemia by proteomic analysis.
abstract: The immune system plays an important role in the treatment of chronic myeloid leukemia (CML). Identification of leukemia-associated antigens (LAAs) eliciting an immune response in patients is a prerequisite for specific immunotherapy of CML. To identify new LAAs in CML, We utilized a novel approach based serology and proteomics technologies. LAAs were identified by comparing the reactivity of proteins resolved by 2-DE with sera from CML patients and healthy donors. Several new LAAs were identified including alpha enolase, aldolase A, HSP70 protein8, beta-tubulin and tropomyosin isoforms. Although, the functions of these identified proteins in CML need further investigation, the detection of autoantibodies in CML may have value on CML screening, diagnosis, or follow-up. Additionally, identification of LAAs in CML may also be of vital importance in antigen-based immunotherapy.
authors.affiliation: Institute of Immunology of PLA, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zou
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Pei
authors.forename: Daping
authors.initials: D
authors.lastname: Zhong
authors.forename: Miao
authors.initials: M
authors.lastname: Gen
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Jiehong
authors.initials: J
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Mou
authors.forename: Junfeng
authors.initials: J
authors.lastname: Han
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhi
mesh_terms: D000293:Adolescent; D000328:Adult; D000951:Antigens, Neoplasm / Q000032:analysis*; D001323:Autoantibodies / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D014335:Tropomyosin / Q000032:analysis; D014404:Tubulin / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins; D014335:Tropomyosin; D014404:Tubulin; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase