title: Acute dopamine/norepinephrine reuptake inhibition increases brain and core temperature in rats.
abstract: The purpose of the present study was to examine the effects of an acute dose of the dual dopamine (DA) and norepinephrine (NE) reuptake inhibitor bupropion (Bup) on brain (T(brain)), body core (T(core)), and tail skin (T(tail)) temperature in freely moving rats and to simultaneously monitor the extracellular neurotransmitter concentrations in the preoptic area and anterior hypothalamus (PO/AH). A microdialysis probe was inserted in the PO/AH, and samples for NE, DA, and serotonin (5-HT) were collected every 20 min before and after the injection of 17 mg/kg of Bup, for a total sampling time of 180 min. T(core) was monitored using a biotelemetry system. T(brain) and T(tail), an index of heat loss response, were also measured. Both NE and DA levels in the PO/AH significantly increased after Bup injection compared with the baseline levels, reaching approximately 450 and 230%, respectively, 40 min after injection. There was no effect on 5-HT release. The neurotransmitter changes were accompanied by a significant decrease in T(tail) and an increase in both T(brain) and T(core) compared with the baseline levels. The present results demonstrate that inhibition of NE and DA reuptake suppresses heat loss mechanisms and elevates T(brain) and T(core) in freely moving rats.
authors.affiliation: Department of Human Physiology and Sportsmedicine, Vrije Universiteit Brussel, Belgium.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Romain
authors.initials: R
authors.lastname: Meeusen
authors.forename: Sophie
authors.initials: S
authors.lastname: Sarre
authors.forename: Marc
authors.initials: M
authors.lastname: Diltoer
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Piacentini
authors.forename: Yvette
authors.initials: Y
authors.lastname: Michotte
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016642:Bupropion / Q000008:administration & dosage / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D045604:Extracellular Fluid / Q000378:metabolism; D007032:Hypothalamus, Anterior / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D017551:Microdialysis; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism / Q000502:physiology*; D009994:Osmolar Concentration; D011301:Preoptic Area / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012881:Skin Temperature / Q000187:drug effects; D013623:Tail; D013686:Telemetry
chemical_list: D018765:Dopamine Uptake Inhibitors; D014179:Neurotransmitter Uptake Inhibitors; D016642:Bupropion; D004298:Dopamine; D009638:Norepinephrine