title: Developmental control of CD8 T cell-avidity maturation in autoimmune diabetes.
abstract: The progression of immune responses is generally associated with an increase in the overall avidity of antigen-specific T cell populations for peptide-MHC. This is thought to result from preferential expansion of high-avidity clonotypes at the expense of their low-avidity counterparts. Since T cell antigen-receptor genes do not mutate, it is puzzling that high-avidity clonotypes do not predominate from the outset. Here we provide a developmental basis for this phenomenon in the context of autoimmunity. We have carried out comprehensive studies of the diabetogenic CD8 T cell population that targets residues 206-214 of the beta cell antigen islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP(206-214)) and undergoes avidity maturation as disease progresses. We find that the succession of IGRP(206-214)-specific clonotypes with increasing avidities during the progression of islet inflammation to overt diabetes in nonobese diabetic mice is fueled by autoimmune inflammation but opposed by systemic tolerance. As expected, naive high-avidity IGRP(206-214)-specific T cells respond more efficiently to antigen and are significantly more diabetogenic than their intermediate- or low-avidity counterparts. However, central and peripheral tolerance selectively limit the contribution of these high-avidity T cells to the earliest stages of disease without abrogating their ability to progressively accumulate in inflamed islets and kill beta cells. These results illustrate the way in which incomplete deletion of autoreactive T cell populations of relatively high avidity can contribute to the development of pathogenic autoimmunity in the periphery.
authors.affiliation: Department of Microbiology and Infectious Diseases, Julia McFarlane Diabetes Research Centre, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Bingye
authors.initials: B
authors.lastname: Han
authors.forename: Pau
authors.initials: P
authors.lastname: Serra
authors.forename: Jun
authors.initials: J
authors.lastname: Yamanouchi
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Amrani
authors.forename: John F
authors.initials: JF
authors.lastname: Elliott
authors.forename: Peter
authors.initials: P
authors.lastname: Dickie
authors.forename: Teresa P
authors.initials: TP
authors.lastname: Dilorenzo
authors.forename: Pere
authors.initials: P
authors.lastname: Santamaria
mesh_terms: D000818:Animals; D015551:Autoimmunity; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D005952:Glucose-6-Phosphatase / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology; D011506:Proteins / Q000276:immunology; D017634:Self Tolerance
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D005952:Glucose-6-Phosphatase; C117983:G6pc2 protein, mouse