Compact detector for proteins based on two-photon excitation of native fluorescence.
A compact, inexpensive detector for proteins has been constructed based on two-photon excitation of fluorescence from phenylalanine, tyrosine, and tryptophan. The fluorescence was excited by a solid-state microchip laser operating at 532 nm. Detection limits for phenylalanine, tyrosine, and tryptophan were 62 microM, 2.0 microM, and 470 nM, respectively, in a volume of 3 fL. The detection limit for a test protein, bovine serum albumin, was 130 nM.
Paul
Uchenna P
UP
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, USA.
Li
Li
L
Lee
Milton L
ML
Farnsworth
Paul B
PB
D000818:Animals; D002417:Cattle; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005453:Fluorescence; D056656:Lab-On-A-Chip Devices; D007834:Lasers; D046208:Microchip Analytical Procedures / Q000379:methods; D055095:Optics and Photonics; D010649:Phenylalanine / Q000032:analysis / Q000528:radiation effects; D017785:Photons; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000528:radiation effects; D013997:Time Factors; D014364:Tryptophan / Q000032:analysis / Q000528:radiation effects; D014443:Tyrosine / Q000032:analysis / Q000528:radiation effects
D012710:Serum Albumin, Bovine; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan