title: Alteration of human neuroblastoma cell morphology and neurite extension with micropatterns.
abstract: The spatial orientation of nerve cells plays a pivotal role in nerve regeneration. Here we report a new method for regulating neuronal cell morphology and guiding neurite extension on standard tissue culture dishes. Random copolymers of oligoethyleneglycol methacrylate and methacrylic acid [poly(OEGMA-co-MA)], microcontact printed on standard tissue culture dishes, resist cell attachment and remain intact in serum-containing medium for up to 2 weeks. Cell viability assay of SH-SY5Y cells demonstrated that poly(OEGMA-co-MA) on the substrate or in solution has no cytotoxic effect. When retinoic acid was added to SH-SY5Y cells, they extended neurites along the line patterns that are significantly longer than cells cultured on non-patterned culture dishes. The ability to guide neurite extension with micrometer precision is valuable for guiding directional growth of neurites and path finding of regenerating nerves.
authors.affiliation: Department of Chemical and Material Engineering, University of Cincinnati, USA.
authors.forename: In Hong
authors.initials: IH
authors.lastname: Yang
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Co
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Ho
mesh_terms: D000199:Actins / Q000737:chemistry; D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D013379:Substrate Specificity; D013997:Time Factors; D014212:Tretinoin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D003470:Culture Media; D011108:Polymers; D014212:Tretinoin