Influence of KfoG on capsular polysaccharide structure in Escherichia coli K4 strain.
An enzyme KfoG with unknown function is coded by the gene kfoG. Gene kfoG belongs to genes from region 2, which are responsible for structure of capsular polysaccharide. Only two enzymes, KfoG and KfoC, coded by genes from region 2, have a glycosyltransferase motif. KfoC is the bifunctional enzyme, which is able to add both GalNAc and GlcUA on nascent polysaccharide, termed chondroitin polymerase. KfoG was predicted to be a fructosyltransferase. The gene that codes the KfoG enzyme was disrupted using homological recombination and absence of this gene was confirmed on both DNA and RNA levels. After disruption no structural changes have been observed, what indicates that fructose branching of the chondroitin backbone is not caused by enzymes, which are coded by genes from region 2 of the K4 capsular gene cluster.
Krahulec
Ján
J
CPN spol. s r.o., Dolní Dobrou 401, 56102 Czech Republic. krahulec@contipro.cz
Krahulcová
Jana
J
Medová
Martina
M
Velebny
Vladimír
V
D016667:Bacterial Capsules; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D005632:Fructose / Q000737:chemistry / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D016695:Glycosyltransferases / Q000235:genetics / Q000502:physiology*; D006602:Hexosyltransferases / Q000235:genetics / Q000502:physiology*; D016254:Mutagenesis, Insertional / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D001426:Bacterial Proteins
D029968:Escherichia coli Proteins; D005632:Fructose; D016695:Glycosyltransferases; D006602:Hexosyltransferases; D011135:Polysaccharides, Bacterial; C063323:capsular polysaccharide K4; C504138:KfoG protein, E coli; C021451:inulosucrase; D001426:Bacterial Proteins