Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma.
OBJECTIVE
To study the effect of IGF-1/IGF-1R and gastrin/CCK-BR on carcinogenesis and development of human gastric carcinoma and to explore its mechanism and provide a credible theoretical foundation for early diagnosis and molecular therapy of gastric carcinoma.


METHODS
mRNA expression levels of IGF-1/IGF-1R and gastrin/CCK-BR were assessed by RT-PCR method in gastric cancer tissues, adjacent mucosa, and tumor-free tissues from 56 patients with gastric carcinoma and normal gastric mucosae from 56 healthy controls. Tissue specimens were obtained by biopsy and confirmed by histological evaluation.


RESULTS
The mRNA levels of IGF-1/IGF-1R were increased in gastric cancer tissues compared with normal tissues from healthy controls and successively increased in tumor-free tissues, adjacent mucosa, and gastric cancer tissues. The mRNA levels of gastrin/CCK-BR were increased in gastric cancer tissues compared with normal tissues from healthy controls. There was a significant difference between gastric cancer tissues and adjacent mucosa and tumor-free tissues, but the mRNA levels of gastrin were not significantly increased in adjacent mucosa and gastric cancer tissues compared with tumor-free tissues. The mRNA levels of CCK-BR were increased in gastric cancer tissues and adjacent mucosa compared with tumor-free tissues, but not significantly increased in adjacent mucosa and gastric cancer tissues compared with gastric cancer tissues.


CONCLUSIONS
Overexpression of IGF-1/IGF-1R and gastrin/CCK-BR promotes the disorderly proliferation of gastric mucosa epithelia and it is of great significance in the carcinogenesis and development of gastric carcinoma.
Zhao
Ming-Dong
MD
Department of Radiology, Affiliated Hospital of Binzhou Medical College, Binzhou 256-603, Shandong Province, China.
Hu
Xue-Mei
XM
Sun
Dian-Jing
DJ
Zhang
Qun
Q
Zhang
Yu-Hao
YH
Meng
Wei
W
D005755:Gastrins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D043887:Receptor, Cholecystokinin B / Q000235:genetics*; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
D005755:Gastrins; D043887:Receptor, Cholecystokinin B; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1