title: Evaluation of inoculum of Candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate.
abstract: The effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of Candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/L), xylose (30 g/L), or a mixture of glucose (2 g/L) and xylose (30 g/L). The inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 IU/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0.203 IU/mgprot) when compared with an inoculum-grown medium containing only xylose. The improvement in enzyme activities resulted in higher values of xylitol yield (0.56 g/g) and productivity (0.46 g/[L.h]) after 48 h of fermentation.
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering of Lorena, FAENQUIL, Rodovia Itajuba-Lorena, Km 74.5, Caixa Postal 116, 12600-970, Lorena, SP, Brazil.
authors.forename: Débora Danielle Virgínio
authors.initials: DD
authors.lastname: da Silva
authors.forename: Maria
authors.initials: M
authors.lastname: das Graças de Almeida Felipe
authors.forename: Ismael Maciel
authors.initials: IM
authors.lastname: de Mancilha
authors.forename: Sílvio Silvério
authors.initials: SS
authors.lastname: da Silva
mesh_terms: D000449:Aldehyde Reductase / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003470:Culture Media / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D050542:D-Xylulose Reductase; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D014993:Xylitol / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D014994:Xylose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D005947:Glucose; D014993:Xylitol