title: [Sequences and their polymorphisms of swine MHC-DQB, DRB proximal promoter region].
abstract: The primers, RF/RR and QF1/QR,were designed based on the homologous region of swine MHC-DQB and DRB exon1 sequences and HLA-DQB and HLA-DRB sequences. The 5' proximal promoter region (URR) nucleotide sequences of swine MHC-DQB and DRB were firstly obtained by PCR, cloning and sequencing techniques. There exist highly conserved sequence motifs including W, X, Y, CCAAT, and TATA-like boxes in the obtained sequences,showing the same organization of the conserved regulatory elements as in other species. 12 DRB-URR and 14 DQB-URR alleles were found by PCR-SSCP in 313 pigs. The aligned results of these alleles showed that there exist abundant polymorphism sites in both Porcine MHC-DQB and DRB proximal promoter region.
authors.affiliation: College of Animal Science & Technology, China Agricuutural Univeisity, Beijing 100094, China.
authors.forename: Ru-Hai
authors.initials: RH
authors.lastname: X U
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Dong-Xiao
authors.initials: DX
authors.lastname: Sun
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Ding
authors.forename: Pei-Qiong
authors.initials: PQ
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*; D016385:TATA Box
chemical_list: D000949:Histocompatibility Antigens Class II