No association of polymorphisms in the glutathione S-transferase genes with pre-eclampsia, eclampsia and HELLP syndrome in a Turkish population.
OBJECTIVE
There is substantial evidence that genetic factors play a role in pre-eclampsia. The aim of this study was to determine whether genetic variability in the encoding of genes for glutathione S-transferase M1 (GSTM1) and glutathione S-transferase T1 (GSTT1) contributes to individual differences in susceptibility to pre-eclampsia, eclampsia, or hemolysis, elevated liver enzymes, and low platelets (HELLP syndrome).


METHODS
A total of 221 women with pre-eclampsia, eclampsia and HELLP syndrome and 147 healthy female controls were genotyped for GSTM1 and GSTT1 polymorphisms by polymerase chain reaction (PCR). Statistical evaluation of differences in polymorphic rates was carried out using chi(2) analysis.


RESULTS
This study included 140 pre-eclamptic, 33 eclamptic and 48 HELLP syndrome cases and 147 healthy controls. The frequencies for the GSTM1 null genotype were 58%, 45%, and 60% for pre-eclampsia, eclampsia, and HELLP syndrome, respectively, and in controls it was 55%. The distribution of the GSTT1 null genotype was 22%, 21%, and 27% for pre-eclampsia, eclampsia, and HELLP syndrome, respectively, and in controls it was 22%. There was no significant association between GSTM1 and GSTT1 polymorphisms and pre-eclampsia, eclampsia, and HELLP syndrome.


CONCLUSIONS
Our data do not support a role for polymorphisms of the GSTM1 and GSTT1 genes in the pathogenesis of pre-eclampsia, eclampsia and HELLP syndrome.
Cetin
Meral
M
Department of Obstetrics and Gynecology, Cumhuriyet University School of Medicine, Sivas, Turkey. mcetin@cumhuriyet.edu.tr
Pinarbasi
Ergun
E
Percin
Ferda E
FE
Akgün
Egemen
E
Percin
Sibel
S
Pinarbasi
Hatice
H
Gurlek
Fatma
F
Cetin
Ali
A
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D017931:DNA Primers; D004461:Eclampsia / Q000235:genetics*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D017359:HELLP Syndrome / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics; D011247:Pregnancy; D014421:Turkey / Q000453:epidemiology; D044465:White People / Q000235:genetics*
D017931:DNA Primers; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1