title: Human immune response to a plague vaccine comprising recombinant F1 and V antigens.
abstract: The human immune response to a new recombinant plague vaccine, comprising recombinant F1 (rF1) and rV antigens, has been assessed during a phase 1 safety and immunogenicity trial in healthy volunteers. All the subjects produced specific immunoglobulin G (IgG) in serum after the priming dose, which peaked in value after the booster dose (day 21), with the exception of one individual in the lowest dose level group, who responded to rF1 only. Three subjects, found to have an anti-rV titer at screening, were excluded from the overall analysis. Human antibody functionality has been assessed by quantification of antibody competing for binding to rV in vitro and also by the transfer of protective immunity in human serum into the naive mouse. Human and macaque IgG competed for binding to rV in vitro with a mouse monoclonal antibody, previously shown to protect mice against challenge with plague, suggesting that this protective B-cell epitope on rV is conserved between these three species. Total IgG to rV in individuals and the titer of IgG competing for binding to rV correlated significantly at days 21 (r = 0.72; P < 0.001) and 28 (r = 0.82; P < 0.001). Passive transfer of protective immunity into mice also correlated significantly with total IgG titer to rF1 plus rV at days 21 (r(2) = 98.6%; P < 0.001) and 28 (r(2) = 76.8%; P < 0.03). However, no significant vaccination-related change in activation of peripheral blood mononuclear cells was detected at any time. Potential serological immune correlates of protection have been investigated, but no trends specific to vaccination could be detected in cellular markers.
authors.affiliation: Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom. dwilliamson@dstl.gov.uk
authors.forename: E D
authors.initials: ED
authors.lastname: Williamson
authors.forename: H C
authors.initials: HC
authors.lastname: Flick-Smith
authors.forename: C
authors.initials: C
authors.lastname: Lebutt
authors.forename: C A
authors.initials: CA
authors.lastname: Rowland
authors.forename: S M
authors.initials: SM
authors.lastname: Jones
authors.forename: E L
authors.initials: EL
authors.lastname: Waters
authors.forename: R J
authors.initials: RJ
authors.lastname: Gwyther
authors.forename: J
authors.initials: J
authors.lastname: Miller
authors.forename: P J
authors.initials: PJ
authors.lastname: Packer
authors.forename: M
authors.initials: M
authors.lastname: Irving
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D007958:Leukocyte Count; D008297:Male; D010931:Plague Vaccine / Q000276:immunology*; D052899:Pore Forming Cytotoxic Proteins; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C043908:LcrV protein, Yersinia; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D014614:Vaccines, Synthetic; C086725:caf1 protein, Yersinia pestis