title: The lantibiotic lacticin 3147 produced in a milk-based medium improves the efficacy of a bismuth-based teat seal in cattle deliberately infected with Staphylococcus aureus.
abstract: A preparation of the bacteriocin lacticin 3147 (prepared from a demineralized whey protein fermentation liquor) was combined as a powder with a bismuth-based intramammary teat seal and evaluated for its potential as an antimicrobial in non-lactating cows. The lacticin/teat seal formulation enabled significant bacteriocin release from the seal without the requirement for a surfactant. Studies in vivo in lactating cows demonstrated that this formulation was effective in reducing bacterial recoveries (approximately 20-fold) from teats deliberately inoculated with Staphylococcus aureus after infusion. Moreover, this formulation also significantly reduced the numbers of Staph. aureus recovered from teats that were exposed to the challenge bacterium before the infusion of the teat seal preparation. The powdered preparation of lacticin 3147 did, however, cause some teat irritation as evidenced by associated rises in somatic cell count (SCC). However, this effect was short-lived and when the mean SCC readings pre-infusion and the final two readings post-infusion were compared, there was no significant difference in the immunological acceptance between treatments.
authors.affiliation: Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork.
authors.forename: Fiona
authors.initials: F
authors.lastname: Crispie
authors.forename: Denis
authors.initials: D
authors.lastname: Twomey
authors.forename: James
authors.initials: J
authors.lastname: Flynn
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
authors.forename: Paul
authors.initials: P
authors.lastname: Ross
authors.forename: William
authors.initials: W
authors.lastname: Meaney
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D001729:Bismuth / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial / Q000662:veterinary; D003470:Culture Media; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; C082508:lacticin 481; D001729:Bismuth