title: Solution stirring initiates nucleation and improves the quality of adenosine deaminase crystals.
abstract: Crystals of bovine adenosine deaminase (ADA) grown over a two-week period in the presence of an inhibitor (ADA complex) were found to be of low quality for X-ray diffraction analysis. Furthermore, ADA incubated in the absence of an inhibitor (ADA native) did not form any crystals using conventional crystallization methods. A solution-stirring technique was used to obtain high-quality ADA complex and ADA native crystals. The crystals obtained using this technique were found to be of high quality and were shown to have high structural resolution for X-ray diffraction analyses. The results reported here indicate that the solution-stirring technique promotes nucleation and improves the quality of protein crystals.
authors.affiliation: Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. adachi@pwr.eng.osaka-u.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Ai
authors.initials: A
authors.lastname: Niino
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Masaichi
authors.initials: M
authors.lastname: Warizaya
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Takatomo
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D012996:Solutions / Q000737:chemistry
chemical_list: D012996:Solutions; D000243:Adenosine Deaminase