Holliday junction dynamics and branch migration: single-molecule analysis.
The Holliday junction (HJ) is a central intermediate in various genetic processes including homologous and site-specific recombination and DNA replication. Branch migration allows the exchange between homologous DNA regions, but the detailed mechanism for this key step of DNA recombination is unidentified. Here, we report direct real-time detection of branch migration in individual molecules. Using appropriately designed HJ constructs we were able to follow junction branch migration at the single-molecule level. Branch migration is detected as a stepwise random process with the overall kinetics dependent on Mg2+ concentration. We developed a theoretical approach to analyze the mechanism of HJ branch migration. The data show steps in which the junction flips between conformations favorable to branch migration and conformations unfavorable to it. In the favorable conformation (the extended HJ geometry), the branch can migrate over several base pairs detected, usually as a single large step. Mg2+ cations stabilize folded conformations and stall branch migration for a period considerably longer than the hopping step. The conformational flip and the variable base pair hopping step provide insights into the regulatory mechanism of genetic processes involving HJs.
Karymov
Mikhail
M
School of Life Sciences, and Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
Daniel
Douglas
D
Sankey
Otto F
OF
Lyubchenko
Yuri L
YL
D001483:Base Sequence; D002413:Cations, Divalent / Q000378:metabolism / Q000494:pharmacology; D045566:DNA, Cruciform / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008954:Models, Biological; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011995:Recombination, Genetic / Q000187:drug effects
D002413:Cations, Divalent; D045566:DNA, Cruciform; D008274:Magnesium