title: Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus.
abstract: Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.
authors.affiliation: Department of Biological Sciences, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA.
authors.forename: Salvador
authors.initials: S
authors.lastname: Hernández-Martínez
authors.forename: Yiping
authors.initials: Y
authors.lastname: Li
authors.forename: Humberto
authors.initials: H
authors.lastname: Lanz-Mendoza
authors.forename: Mario H
authors.initials: MH
authors.lastname: Rodríguez
authors.forename: Fernando G
authors.initials: FG
authors.lastname: Noriega
mesh_terms: D000330:Aedes / Q000737:chemistry*; D000818:Animals; D000852:Anopheles / Q000737:chemistry*; D009033:Culicidae / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000378:metabolism*; D018613:Microscopy, Confocal; D009479:Neuropeptides / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; C050669:allatotropin