Association of interleukin 18 (IL18) polymorphisms with specific IgE levels to mite allergens among asthmatic patients.
BACKGROUND
Allergy is regarded as a multifactorial condition. Its onset and severity are influenced by both genetic and environmental factors. Identification of genetic factors involved in asthma development and related phenotypes is a major task in understanding the genetic background of asthma. The possible involvement of IL18 polymorphisms in asthma was examined in a Korean asthma cohort.


METHODS
Direct sequencing was performed to discover single-nucleotide polymorphisms (SNPs) in the IL18 gene. Single-base extension (SBE) method was employed for genotyping. Genotypic influence of IL18 was analysed using logistic and multiple-regression models.


RESULTS
Although no polymorphisms in the IL18 gene showed significant association with the risk of asthma development, analyses of the association with specific serum IgE levels to Dermatophagoides farinae (D.f.) and D. pteronyssinus (D.p.) among asthmatic patients revealed significant associations with two completely linked SNPs, i.e. -148G>C and +13925A>C(Ser35Ser) (P = 0.01-0.11 for D.f. and P = 0.005-0.11 for D.p.). Both C allele of -148G>C and C allele of +13925A>C showed gene dose-dependent effects on the levels of specific IgE. The lowest IgE levels in homozygotes of minor alleles (1.13 and 1.22 of D.f.; 1.38 and 1.33 of D.p., respectively), intermediate IgE levels in heterozygotes (1.60 and 1.70 of D.f.; 1.84 and 1.92 of D.p., respectively), and the highest levels in homozygotes for major allele (1.93 and 1.93 of D.f.; 2.24 and 2.24 of D.p., respectively), were found.


CONCLUSIONS
The genetic relevance of IL18 to specific IgE might offer an important step in understanding the genetic background of allergic diseases.
Shin
H D
HD
Department of Genetic Epidemiology, SNP Genetics, Inc., MaeHun B/D, Seoul, South Korea.
Kim
L H
LH
Park
B L
BL
Choi
Y H
YH
Park
H-S
HS
Hong
S-J
SJ
Choi
B W
BW
Lee
J H
JH
Park
C-S
CS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D002648:Child; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D020382:Interleukin-18 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D039981:Pyroglyphidae / Q000276:immunology*
D000485:Allergens; D020382:Interleukin-18; D007073:Immunoglobulin E