In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway.
A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes.
Companjen
Arjen
A
Cell Biology and Immunology Group, Wageningen University, 6709 PG Wageningen, The Netherlands.
Heinhuis
Bas
B
Aspers
Kelly
K
Rombout
Jan
J
D000818:Animals; D002118:Calcium / Q000276:immunology; D002347:Carps / Q000276:immunology*; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods* / Q000662:veterinary*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004533:Egtazic Acid; D007770:L-Lactate Dehydrogenase; D008562:Membrane Glycoproteins / Q000276:immunology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins
D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin; D004533:Egtazic Acid; D007770:L-Lactate Dehydrogenase; D002118:Calcium