title: Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells.
abstract: The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5'-triphosphate (ATP), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non-migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relationship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.
authors.affiliation: Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, China.
authors.forename: Jian Wen
authors.initials: JW
authors.lastname: Mao
authors.forename: Li Wei
authors.initials: LW
authors.lastname: Wang
authors.forename: Tim
authors.initials: T
authors.lastname: Jacob
authors.forename: Xue Rong
authors.initials: XR
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Lin Yan
authors.initials: LY
authors.lastname: Zhu
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Si Huai
authors.initials: SH
authors.lastname: Nie
authors.forename: Li Xin
authors.initials: LX
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D020533:Angiogenesis Inhibitors / Q000378:metabolism; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D018118:Chloride Channels / Q000378:metabolism; D006801:Humans; D007038:Hypotonic Solutions; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D009579:Nitrobenzoates / Q000378:metabolism; D013629:Tamoxifen / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D020533:Angiogenesis Inhibitors; D018931:Antineoplastic Agents, Hormonal; D018118:Chloride Channels; D007038:Hypotonic Solutions; D009579:Nitrobenzoates; D013629:Tamoxifen; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D000255:Adenosine Triphosphate