title: A novel molecular marker of pituitary tumor transforming gene involves in a rat liver regeneration.
abstract: BACKGROUND
  Pituitary tumor transforming gene (PTTG), homologous to a mammalian securin, plays a pivotal role in cell transformation, however, its biological function(s) in normal tissues is not fully understood. Because the liver is a regenerative organ, the relevant biological function of PTTG in the liver would be more feasible to understand PTTG. Also, PTTG may be involved in the liver regeneration.
  
  
  METHODS
  Expressions of rat hepatic PTTG messengerRNA (mRNA) and cellular immunoreactivities during the cell proliferative period of the liver regeneration both in vitro and in vivo were tested.
  
  
  RESULTS
  PTTG expression of the rat primary hepatocyte was stimulated by HGF in a dose dependent manner, and was suppressed when hepatocyte proliferation was inhibited by transforming growth factor-beta1. A positive PTTG immunoreactive co-localizing with 5-bromo-2'-deoxyuridine (BrdU) in the hepatocyte nucleus was found and there was a concurrent sister chromatin itself by the immunofluorescent labeling of PTTG with cytokeratin 18 (CK18).
  
  
  CONCLUSIONS
  Since the correlation of PTTG mRNA expression, cell proliferation and immunoreactivity were observed in primary rat cultured hepatocytes, PTTG may be a novel marker of cell proliferation both in vitro and in vivo liver regeneration.
authors.affiliation: Department of Medicine, Cedars-Sinai Research Institute UCLA School of Medicine, Los Angeles, California, USA.
authors.forename: Kozo
authors.initials: K
authors.lastname: Akino
authors.forename: Sadanori
authors.initials: S
authors.lastname: Akita
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuguchi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takumi
authors.forename: Run
authors.initials: R
authors.lastname: Yu
authors.forename: Xhi-yong
authors.initials: XY
authors.lastname: Wang
authors.forename: Jacek
authors.initials: J
authors.lastname: Rozga
authors.forename: Achilles A
authors.initials: AA
authors.lastname: Demetriou
authors.forename: Shlomo
authors.initials: S
authors.lastname: Melmed
authors.forename: Akira
authors.initials: A
authors.lastname: Ohtsuru
authors.forename: Shunichi
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D015415:Biomarkers; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D015870:Gene Expression / Q000187:drug effects; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008115:Liver Regeneration / Q000235:genetics*; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D064249:Securin; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D064249:Securin; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor; D007633:Keratins; D001973:Bromodeoxyuridine