title: Discovery of selective aldo-keto reductase ligands--an on-bead assay strategy.
abstract: An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
authors.affiliation: Department of Chemistry, University of California, Davis, 95616-5295, USA.
authors.forename: Seth
authors.initials: S
authors.lastname: Dixon
authors.forename: Lori
authors.initials: L
authors.lastname: Robins
authors.forename: Robert A
authors.initials: RA
authors.lastname: Elling
authors.forename: Ruiwu
authors.initials: R
authors.lastname: Liu
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.forename: David K
authors.initials: DK
authors.lastname: Wilson
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Kurth
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D000449:Aldehyde Reductase / Q000378:metabolism; D000074408:Aldo-Keto Reductases; D008024:Ligands; D008856:Microscopy, Fluorescence; D008958:Models, Molecular
chemical_list: D008024:Ligands; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase