Agrobacterium phytochrome as an enzyme for the production of ZZE bilins.
Photoconversion of phytochrome from the red-absorbing form Pr to the far-red-absorbing form Pfr is initiated by a Z to E isomerization around the ring C-ring D connecting double bond; the chromophore undergoes a ZZZ to ZZE isomerization. In vivo, phytochrome chromophores are covalently bound to the protein, but several examples of noncovalent in vitro adducts have been reported which also undergo Pr to Pfr photoconversion. We show that free biliverdin or phycocyanobilin, highly enriched in the ZZE isomer, can easily be obtained from chromophores bound in a noncovalent manner to Agrobacterium phytochrome Agp1, and used for spectral assays. Photoconversion of free biliverdin in a methanol/HCl solution from ZZE to ZZZ proceeded with a quantum yield of 1.8%, but was negligible in neutral methanol solution, indicating that this process is proton-dependent. The ZZE form of biliverdin and phycocyanobilin were tested for their ability to assemble with Agp1 and cyanobacterial phytochrome Cph1, respectively. In both cases, a Pfr-like adduct was formed but the chromophore was bound in a noncovalent manner to the protein. Agp1 Pfr undergoes dark reversion to Pr; the same feature was found for the noncovalent ZZE adduct. After dark reversion, the chromophore became covalently bound to the protein. In analogy, the PCB chromophore became covalently bound to Cph1 upon irradiation with strong far-red light which initiated ZZE to ZZZ isomerization. Agrobacterium Agp2 belongs to a yet small group of phytochromes which also assemble in the Pr form but convert from Pr to Pfr in darkness. When the Agp2 apoprotein was assembled with the ZZE form of biliverdin, the formation of the final adduct was accelerated compared to the formation of the ZZZ control, indicating that the ZZE chromophore fits directly into the chromophore pocket of Agp2.
Lamparter
Tilman
T
Pflanzenphysiologie, Freie Universität Berlin, Königin Luise Strasse 12-16, D-14195 Berlin, Germany. lamparte@zedat.fu-berlin.de
Michael
Norbert
N
D016960:Agrobacterium tumefaciens / Q000201:enzymology* / Q000235:genetics; D000409:Alanine / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001654:Bile Pigments / Q000096:biosynthesis*; D001664:Biliverdine / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000235:genetics; D007536:Isomerism; D008027:Light; D000432:Methanol / Q000737:chemistry; D010777:Photochemistry; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D010798:Phycocyanin / Q000737:chemistry / Q000378:metabolism; D010834:Phytochrome / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011758:Pyrroles / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D045725:Tetrapyrroles
D001426:Bacterial Proteins; D001654:Bile Pigments; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D011758:Pyrroles; D045725:Tetrapyrroles; D010798:Phycocyanin; D010834:Phytochrome; C011069:phycocyanobilin; D011494:Protein Kinases; C413623:Cph1 phytochrome protein, bacteria; D003545:Cysteine; D001664:Biliverdine; D000409:Alanine; D000432:Methanol