Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements.
Galpha(12/13) have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Galpha(12) and Galpha(13) signaling by overexpressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Galpha(12/13) are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, Galpha(12/13) function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
Lin
Fang
F
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Sepich
Diane S
DS
Chen
Songhai
S
Topczewski
Jacek
J
Yin
Chunyue
C
Solnica-Krezel
Lilianna
L
Hamm
Heidi
H
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D044365:GTP-Binding Protein alpha Subunits, G12-G13 / Q000235:genetics / Q000378:metabolism*; D005775:Gastrula / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
D036341:Intercellular Signaling Peptides and Proteins; D043562:Receptors, G-Protein-Coupled; D051153:Wnt Proteins; D044365:GTP-Binding Protein alpha Subunits, G12-G13