title: [PC-12 cells hydrolyze the fibrin clot by producing both plasminogen and its tissue activator].
abstract: It has been shown that rat pheochromocytoma PC-12 cells degraded fibrin clots in vitro. SDS-PAGE performed in the gels of different density and Western blotting using monoclonal antibodies against DD- and D-fibrin fragments demonstrated that both high and low molecular weight degradation products similar to those of fibrin hydrolysis by plasmin had been formed. Enzyme electrophoresis, chromogenic assay and enzyme-linked immunosorbent assay using tPA-specific antibodies demonstrated that PC-12 cells constitutively secreted both plasminogen and tPA. The results obtained allow using PC-12 cells as a model to examine the interaction of nerve cells with the fibrin clot.
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Petrova
authors.forename: O M
authors.initials: OM
authors.lastname: Savchuk
authors.forename: O M
authors.initials: OM
authors.lastname: Kalashnyk
authors.forename: T M
authors.initials: TM
authors.lastname: Platonova
authors.forename: M V
authors.initials: MV
authors.lastname: Skok
authors.forename: S
authors.initials: S
authors.lastname: Cederholm-Williams
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000378:metabolism*; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005342:Fibrinolysis; D006868:Hydrolysis; D016716:PC12 Cells; D010958:Plasminogen / Q000378:metabolism*; D051381:Rats; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D005337:Fibrin; D010958:Plasminogen; D010959:Tissue Plasminogen Activator