title: A newly discovered function for RNase L in regulating translation termination.
abstract: The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We identify and characterize the human translation termination factor eRF3/GSPT1 as an interacting partner of RNase L. We show that interaction of eRF3 with RNase L leads to both increased translation readthrough efficiency at premature termination codons and increased +1 frameshift efficiency at the antizyme +1 frameshift site. On the basis of our results, we present a model describing how RNase L is involved in regulating gene expression by modulating the translation termination process.
authors.affiliation: Department of Molecular Genetics, Microbiology & Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
authors.forename: Florence
authors.initials: F
authors.lastname: Le Roy
authors.forename: Tamim
authors.initials: T
authors.lastname: Salehzada
authors.forename: Catherine
authors.initials: C
authors.lastname: Bisbal
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Dougherty
authors.forename: Stuart W
authors.initials: SW
authors.lastname: Peltz
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism*; D016368:Frameshift Mutation; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D010454:Peptide Termination Factors; D016601:RNA-Binding Proteins; C087971:peptide-chain-release factor 3; D008156:Luciferases; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease