Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR.
The aim of this study was to evaluate real-time PCR (LightCycler) kits for the detection of methicillin-resistant Staphylococcus aureus in enrichment broth using 200 sets of patient screening swab samples. One hundred sets were processed using a lysostaphin/lysozyme extraction method and a further 100 were tested using a column extraction kit. The PCR kits with lysostaphin/lysozyme lysis showed 95.7% sensitivity, 90.8% specificity, and negative and positive predictive values of 98.6% and 75.9%, respectively, compared with 88%, 95.9%, 84.6% and 95.9%, respectively, with the column extraction kit.
Levi
K
K
Department of Microbiology, Molecular Diagnostics and Typing Unit, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK. katrina.levi@qmc.nhs.uk
Towner
K J
KJ
D015373:Bacterial Typing Techniques; D003470:Culture Media; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification*
D003470:Culture Media; D011933:Reagent Kits, Diagnostic