Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells.
The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-alpha expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.
Weng
Tsui-Ying
TY
Department of Medical Laboratory Sciences and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Chen
Lien-Cheng
LC
Shyu
Huey-Wen
HW
Chen
Shun-Hua
SH
Wang
Jen-Ren
JR
Yu
Chun-Keung
CK
Lei
Huan-Yao
HY
Yeh
Trai-Ming
TM
D000818:Animals; D000831:Animals, Newborn; D000998:Antiviral Agents / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D007223:Infant; D007781:Lactoferrin / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D015678:Viral Structural Proteins / Q000378:metabolism*
D000998:Antiviral Agents; D015678:Viral Structural Proteins; D007781:Lactoferrin