title: Expression of functional recombinant mussel adhesive protein type 3A in Escherichia coli.
abstract: Mussel adhesive proteins, including the 20-plus variants of foot protein type 3 (fp-3), have been suggested as potential environmentally friendly adhesives for use in aqueous conditions and in medicine. Here we report the novel production of a recombinant Mytilus galloprovincialis foot protein type 3 variant A (Mgfp-3A) fused with a hexahistidine affinity ligand in Escherichia coli and its approximately 99% purification with affinity chromatography. Recombinant Mgfp-3A showed a superior purification yield and better apparent solubility in 5% acetic acid (prerequisites for large-scale production and practical use) compared to those of the previously reported recombinant M. galloprovincialis foot protein type 5 (Mgfp-5). The adsorption abilities and adhesion forces of purified recombinant Mgfp-3A were compared with those of Cell-Tak (a commercial mussel extract adhesive) and recombinant Mgfp-5 using quartz crystal microbalance analysis and modified atomic force microscopy, respectively. These assays showed that the adhesive ability of recombinant Mgfp-3A was comparable to that of Cell-Tak but lower than that of recombinant Mgfp-5. Collectively, these results indicate that recombinant Mgfp-3A may be useful as a commercial bioadhesive or an adhesive ingredient in medical or underwater environments.
authors.affiliation: Department of Chemical Engineering and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Hwang
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Gim
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000268:Adhesiveness; D000269:Adhesives; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008422:Materials Testing; D008970:Molecular Weight; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013314:Stress, Mechanical; D014014:Tissue Adhesives
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D011506:Proteins; D011994:Recombinant Proteins; D014014:Tissue Adhesives; C037403:adhesive protein, mussel