Mucosal and systemic antibody responses and protection induced by a prime/boost rotavirus-DNA vaccine in a gnotobiotic pig model.
A live rotavirus prime/DNA boost vaccine regimen was evaluated in a gnotobiotic pig model for human rotavirus (HRV) diarrhea. Plasmid DNA expressing rotavirus inner capsid VP6 was administered to pigs intramuscularly (IM) twice after oral priming with attenuated (Att) Wa strain HRV (AttHRV/VP6DNA2x). Other groups included: (1) VP6 DNA IM 2x then AttHRV orally (VP6DNA2x/AttHRV); (2) VP6 DNA IM 3x (VP6DNA3x) and controls. Significant protection (70%) against virus shedding, but lower protection against diarrhea (30%) was achieved only in the AttHRV/VP6DNA2x group after challenge (virulent Wa HRV). The other vaccines (VP6DNA2x/AttHRV and VP6DNA3x) were less effective. Higher protection rates were associated with the highest IgA antibody responses induced by the AttHRV/VP6DNA2x regimen. Interestingly, the VP6 DNA vaccine, although not effective when administered alone, boosted neutralizing and VP4 antibody titers in pigs previously primed with AttHRV, possibly mediated by cross-reactive T helper cells.
Yuan
Lijuan
L
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster OH 44691, USA. yuan.21@osu.edu
Azevedo
Marli S P
MS
Gonzalez
Ana M
AM
Jeong
Kwang-il
KI
Van Nguyen
Trang
T
Lewis
Peggy
P
Iosef
Cristiana
C
Herrmann
John E
JE
Saif
Linda J
LJ
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D003967:Diarrhea / Q000517:prevention & control / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005856:Germ-Free Life; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology*; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007422:Intestines / Q000276:immunology; D007700:Kinetics; D008208:Lymphatic System / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D012401:Rotavirus / Q000276:immunology; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000235:genetics / Q000276:immunology*; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D010948:Viral Plaque Assay; D017201:Virus Shedding
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; D019444:Vaccines, DNA