title: In vitro cultivation and electron microscopy characterization of Trachipleistophora anthropophthera isolated from the cornea of an AIDS patient.
abstract: We describe an in vitro culture technique for a microsporidian isolated from the corneal biopsy of an HIV-infected patient. The corneal biopsy was inoculated into a monolayer culture of fibroblasts derived from newborn mouse brain and incubated at 37 degrees C in an atmosphere of 5% CO2. Minimum essential medium supplemented with 2% fetal bovine serum appeared to be an optimum medium for growth and maintenance of the parasite and for production of large numbers of spores. This microsporidian was identified as Trachipleistophora anthropophthera based on ultrastructural features. It forms two types of sporophorous vesicles and two types of spores simultaneously: polysporous vesicle type I with eight or more oval spores, 3.7-4.0 microm by 2.0-2.3 microm, and bisporous vesicle type II with two round spores, 1.7-2.2 microm by 1.6-2.0 microm in size.
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors.forename: Sandra I
authors.initials: SI
authors.lastname: Juarez
authors.forename: Chaturong
authors.initials: C
authors.lastname: Putaporntip
authors.forename: Somchai
authors.initials: S
authors.lastname: Jongwutiwes
authors.forename: Akitoyo
authors.initials: A
authors.lastname: Ichinose
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yanagi
authors.forename: Hiroji
authors.initials: H
authors.lastname: Kanbara
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000469:parasitology*; D000818:Animals; D018938:Caco-2 Cells; D002460:Cell Line; D002478:Cells, Cultured; D003315:Cornea / Q000469:parasitology*; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007564:Japan; D051379:Mice; D016814:Microsporidia / Q000254:growth & development* / Q000302:isolation & purification / Q000648:ultrastructure; D016881:Microsporidiosis / Q000150:complications / Q000469:parasitology*; D033761:Spores, Protozoan / Q000648:ultrastructure
chemical_list: D003470:Culture Media