Altered expression of p53, Bcl-2 and Bax induced by microcystin-LR in vivo and in vitro.
It has been reported that MC-LR could induce apoptosis in a variety of cell types. Although the induction of oxidative stress and mitochondrial alteration played critical role in MC-LR induced apoptosis, but the exact mechanisms of MC-LR induced apoptosis are still unknown. In spite of extensive studies on MC-LR mediated cell damages, there is little information on the protein expression of p53 and Bcl-2, Bax in vivo and in vitro, which are vital regulator of apoptosis in response to a variety of stimuli. The present study was undertaken to determine the expression level of p53 and Bcl-2, Bax in cultured hepatocytes and rat liver tissues. The results show that MC-LR can increase the expression of p53 and Bax significantly both in vivo and in vitro, however, MC-LR can only decrease the expression of Bcl-2 significantly in vitro and there is no difference observed in vivo. It can be concluded that the expression of p53, Bcl-2 and Bax are involved in the regulation of MC-LR induced apoptosis.
Fu
Wen-yu
WY
Department of Biochemistry and Molecular Biology, School of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Jia-ping
JP
Wang
Xiu-min
XM
Xu
Li-hong
LH
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C057862:cyanoginosin LR