title: Culture medium optimization for acetic acid production by a persimmon vinegar-derived bacterium.
abstract: A new acetic acid-producing microorganism, Acetobacter sp. RKY4, was isolated from Korean traditional persimmon vinegar, and we optimized the culture medium for acetic acid production from ethanol using the newly isolated Acetobacter sp. RKY4. The optimized culture medium for acetic acid production using this microorganism was found to be 40 g/L ethanol, 10 g/L glycerol, 10 g/L corn steep liquor, 0.5 g/L MgSO4.7H2O, and 1.0 g/L (NH4)H2PO4. Acetobacter sp. RKY4 produced 47.1 g/L of acetic acid after 48 h of fermentation in a 250 mL Erlenmeyer flask containing 50 mL of the optimized medium.
authors.affiliation: School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Jin-Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Jong-Sok
authors.initials: JS
authors.lastname: Choo
authors.forename: Young-Jung
authors.initials: YJ
authors.lastname: Wee
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Yun
authors.forename: Hwa-Won
authors.initials: HW
authors.lastname: Ryu
mesh_terms: D019342:Acetic Acid / Q000302:isolation & purification / Q000378:metabolism*; D000091:Acetobacter / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D029788:Diospyros / Q000382:microbiology*; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013045:Species Specificity
chemical_list: D003470:Culture Media; D000431:Ethanol; D005947:Glucose; D019342:Acetic Acid