Chemistry and biology of the ELISPOT assay.
Enzyme-linked immunospot, or ELISPOT, assay allows the detection of low frequencies of cells secreting various molecules. ELISPOT can be used in many areas of research and, because of its high sensitivity, has the potential to become a valuable diagnostic tool. Based on the same "sandwich" immunochemical principles as enzyme-linked immunosorbent assay, ELISPOT is easy to perform and quantify the results. At the same time ELISPOT remains a state-of-the-art technique that requires accuracy, thorough selection of antibodies and detection reagents, and an understanding of the principles of data analysis. This review covers various technical aspects of the ELISPOT assay, including immunochemical principles of the assay, selection of reagents and plates, and troubleshooting recommendations.
Kalyuzhny
Alexander E
AE
R&D Systems Inc., Minneapolis, MN, USA.
D000469:Alkaline Phosphatase; D000906:Antibodies; D003627:Data Interpretation, Statistical; D004797:Enzyme-Linked Immunosorbent Assay / Q000266:history / Q000379:methods* / Q000706:statistics & numerical data; D049673:History, 20th Century; D006735:Horseradish Peroxidase; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007120:Immunochemistry; D007159:Immunologic Tests; D013194:Staining and Labeling
D000906:Antibodies; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase