Expression of peroxisome proliferator-activated receptor-gamma in renal ischemia-reperfusion injury.
The pathogenesis of ischemia-reperfusion injury is known to involve cytokines and particularly surface adhesion molecules, the expression of which initiates the attachment of inflammatory cells. Peroxisome proliferator-activated receptor (PPAR)-gamma is considered an important immunomodulatory factor as well as a fatty acid regulator. In this study, we researched the expression of PPAR-gamma in renal ischemia-reperfusion injury of the rat. The right kidney was harvested and left renal artery and vein were clamped under laparotomy. The kidney was reperfused after 90 minutes of ischemia, and rats were sacrificed at 0, 1.5, 3, 5, 12, and 24 hours after reperfusion. PPAR-gamma expression was analyzed by immunohistochemical staining using monoclonal antibody. In normal kidney, PPAR-gamma staining was weak on endothelial cells, including mesangial cells. On the other hand, PPAR-gamma staining was weak on interstitial cells and strong on collecting ducts of medulla. From 1.5 to 5 hours after reperfusion, PPAR-gamma staining was strong on endothelial cells, moderate on interstitial cells, and strong on collecting ducts. Twelve hours after reperfusion, PPAR-gamma staining was weak on endothelial cells, moderate on interstitial cells, and strong on collecting ducts. PPAR-gamma is induced on collecting ducts, interstitial cells, and endothelial cells in a rat model having renal ischemia-reperfusion injury.
Matsuyama
M
M
Department of Urology, Osaka City University Graduate School of Medicine, Asahi-machi, Osaka, Japan.
Yoshimura
R
R
Hase
T
T
Uchida
J
J
Tsuchida
K
K
Takemoto
Y
Y
Kawahito
Y
Y
Sano
H
H
Nakatani
T
T
D000818:Animals; D004730:Endothelium, Vascular / Q000473:pathology; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000473:pathology; D008297:Male; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000473:pathology*
D047495:PPAR gamma