Association of apolipoprotein J gene 866C-->T polymorphism with preeclampsia and essential hypertension.
The etiology of preeclampsia (PE) is unknown, but endothelial cell injury plays a pivotal role. Moreover, there is a significantly positive association between PE and later hypertension. Apolipoprotein J (apo J) plays an important role in stabilizing cell membranes at diverse fluid-tissue interfaces and might protect vascular endothelium against an attack by some factors in plasma such as active complement complexes. This study investigated the prevalence and possible association of apo J gene in PE and essential hypertension (EH). To screen for polymorphisms of apo J gene in a population in Guizhou, China, by employing polymerase chain reaction combined with denaturing gradient gel electrophoresis, we identified 866C-->T polymorphism in exon 5 and a rare variant 1061C-->T in exon 7. The CT genotype and T-allele frequencies in PE (CT genotype 26%; T allele 13%) and EH (CT genotype 28.57%; T allele 17.14%) were significantly lower than that in the control group (CT geno type 50%; T allele 27%). The results suggested that the 866C-->T polymorphism might be associated with PE and EH. It is plausible that apo J may play a certain role in the predisposition to PE and EH.
Chen
MiaoXin
M
Department of Obstetrics and Gynecology, Affiliated Hospital of Guiyang Medical College, Guiyang, China.
Yuan
ZhuHua
Z
Shan
KeRen
K
D000328:Adult; D000595:Amino Acid Sequence; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D051152:Clusterin / Q000235:genetics*; D003596:Cytosine; D004252:DNA Mutational Analysis; D004586:Electrophoresis; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000453:epidemiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000453:epidemiology / Q000235:genetics; D015995:Prevalence; D013941:Thymine
C495242:CLU protein, human; D051152:Clusterin; D003596:Cytosine; D013941:Thymine