title: Inhibition of gastric cancer-associated angiogenesis by antisense COX-2 transfectants.
abstract: We characterized the effects of selective Cox-2 inhibition on the angiogenesis gastric cancer cell line SGC7901 by stable transfection of antisense Cox-2 cDNA (Cox-2-AS) or pharmacological inhibition by selective cox-2 inhibitor (NS398). The conditioned media obtained from SGC7901 treated with Cox-2-AS or NS398 suppressed the proliferation, migration and tube formation of human umbilical vein endothelial cells. Moreover, both treatments inhibited in vivo angiogenesis. These inhibitions could be partially reversed by the addition of PGE2. Our findings support that selective inhibition of Cox-2 alone plays an instrumental role on gastric cancer associated angiogenesis.
authors.affiliation: Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital 30-32 Ngan Shing Street, Shatin N.T., Hong Kong, SAR, China.
authors.forename: Yuan-Gen
authors.initials: YG
authors.lastname: Fu
authors.forename: Joseph Jy
authors.initials: JJ
authors.lastname: Sung
authors.forename: Kai-Chun
authors.initials: KC
authors.lastname: Wu
authors.forename: Han-Pin
authors.initials: HP
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Martin
authors.initials: M
authors.lastname: Chan
authors.forename: Victor Yw
authors.initials: VY
authors.lastname: Chan
authors.forename: Ka-Kui
authors.initials: KK
authors.lastname: Chan
authors.forename: Dai-Ming
authors.initials: DM
authors.lastname: Fan
authors.forename: Wai K
authors.initials: WK
authors.lastname: Leung
mesh_terms: D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D016373:DNA, Antisense; D018076:DNA, Complementary; D006801:Humans; D008565:Membrane Proteins; D009389:Neovascularization, Pathologic; D009578:Nitrobenzenes / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000473:pathology*; D013449:Sulfonamides / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D016373:DNA, Antisense; D018076:DNA, Complementary; D008565:Membrane Proteins; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases