In vitro generation of mature neutrophils from canine Lin- bone marrow cells.
The major goal of this work was to describe the in vitro generation of mature functional neutrophils derived from a canine enriched haematopoietic progenitor cell population. We have utilised lineage depletion by immunomagnetic selection to isolate a canine haematopoietic progenitor cell population. The physical, immunological, metabolical and morphological methodologies employed in this study have permitted us to isolate and define a cell population enriched in Rh-123low and CD34+ cells. Irradiated pre-established long-term bone marrow cultures (LTBMC) were utilised to determine the self-renewal ability of lineage negative (Lin-) cells, as well as their capacity to differentiate into mature functional neutrophils. The authors demonstrate for the first time that canine neutrophils derived from Lin- cells are able to produce oxyradicals, express a specific neutrophil surface antigen, and contain gelatinase granules. These characteristics enable them to migrate through basement membranes to act as a first line defence mechanism. The fact that these cells are able to differentiate into functional mature cells, and give rise to long-term culture-initiating cells (LTC-IC) after 35 days of culture, allows the authors to assure that the isolated canine enriched haematopoietic cell population exhibit functional characteristics, associated with primitive haematopoietic cells.
León
Leticia G
LG
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
Ostronoff
Luciana K
LK
Fermín
María Luisa
ML
Fragío
Cristina
C
Kremmer
Elisabeth
E
Kolb
Hans-Jochem
HJ
Tejero
Concepción
C
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004285:Dogs / Q000033:anatomy & histology / Q000276:immunology* / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018189:Immunomagnetic Separation; D066298:In Vitro Techniques; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D016897:Respiratory Burst
D018093:Gelatinases