title: Investigation of human serum albumin (HSA) binding specificity of certain photosensitizers related to pyropheophorbide-a and bacteriopurpurinimide by circular dichroism spectroscopy and its correlation with in vivo photosensitizing efficacy.
abstract: A series of pyropheophorbide-a and bacteriopurpurinimides were investigated to understand the correlation between HSA (site II) binding affinity and in vivo photosensitizing activity. In our study, photosensitizers that bound to site II of HSA produced a significant difference in the circular dichroism spectra of the corresponding complexes, especially at Soret band region of the photosensitizers. Our results suggest that CD spectroscopy of the photosensitizer-HSA complexes could be a valuable tool in screening new photosensitizers before evaluating them for in vivo efficacy.
authors.affiliation: Chemistry Division, Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Chen
authors.forename: Razvan
authors.initials: R
authors.lastname: Miclea
authors.forename: Thamarapu
authors.initials: T
authors.lastname: Srikrishnan
authors.forename: Sathyamangalam
authors.initials: S
authors.lastname: Balasubramanian
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Dougherty
authors.forename: Ravindra K
authors.initials: RK
authors.lastname: Pandey
mesh_terms: D000818:Animals; D001665:Binding Sites; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D004337:Drug Carriers / Q000378:metabolism; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry*; D011485:Protein Binding; D011688:Purinones / Q000737:chemistry*; D012709:Serum Albumin / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D004337:Drug Carriers; D017319:Photosensitizing Agents; D011688:Purinones; D012709:Serum Albumin; D002734:Chlorophyll; C040298:pyropheophorbide a