title: Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio).
abstract: Spindlin has been suggested to play an important role during the transition from oocyte maturation to embryo development in mouse, but its homolog similar to the mouse Spindlin in molecular and expression characterization has not been identified up to now in other vertebrates. In this study, a full length of cDNA sequence is cloned and sequenced from the gibel carp (Carassius auratus gibelio). It contains 1240 nucleotides with an open reading frame of 771 nt encoding 257 amino acids. Based on its amino acid sequence alignment and comparison analysis with the known Spin family proteins, the newly cloned Spin is named Carassius auratus gibelio Spindlin (CagSpin). Its product could be detected from mature eggs to blastula embryos, but its content decreased from the two-cell stage, and could not be detected after the gastrula stage. It suggests that the CagSpin should be a maternal protein that is expressed during oocyte maturation, and plays a crucial role in early cleavage of embryogenesis. CagSpin is the first homolog similar to mouse spindlin identified in fish, and also in other vertebrates. GST pull-down assay reveals the first biochemical evidence for the association of CagSpin and beta-tubulin, the microtubule component. Therefore, CagSpin may play important functions by interacting with beta-tubulin and other spindle proteins during oocyte maturation and egg fertilization.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Mei
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Gui
mesh_terms: D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000737:chemistry; D047108:Embryonic Development; D005775:Gastrula / Q000737:chemistry; D006054:Goldfish / Q000196:embryology*; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D010063:Ovum / Q000737:chemistry; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014404:Tubulin / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D014404:Tubulin; C104434:spindlin