Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis.
A laser-induced fluorescence microscopic system based on optical parametric oscillation has been constructed as a tunable detector for microchip analysis. The detection limit of sulforhodamine B (Ex. 520 nm, Em. 570 nm) was 0.2 mumol, which was approximately eight orders of magnitude better than with a conventional fluorophotometer. The system was applied to the determination of fluorescence-labeled DNA (Ex. 494 nm, Em. 519 nm) in a microchannel and the detection limit reached a single molecule. These results showed the feasibility of this system as a highly sensitive and tunable fluorescence detector for microchip analysis.
Kumemura
Momoko
M
Department of Ecosystem Engineering, Graduate School of Engineering, University of Tokushima, 2-1 Minami-jousanjima, Tokushima 770-8506, Japan. momo@iis.u-tokyo.ac.jp
Odake
Tamao
T
Korenaga
Takashi
T
D010582:Bacteriophage lambda / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D005453:Fluorescence; D056656:Lab-On-A-Chip Devices; D007834:Lasers; D046208:Microchip Analytical Procedures / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D012235:Rhodamines / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D012235:Rhodamines; C022027:lissamine rhodamine B