A CFTR chloride channel activator prevents HrpN(ea)-induced cell death in Arabidopsis thaliana suspension cells.
Erwinia amylovora is a necrogenic bacterium that causes fire blight of the Maloideae subfamily of Roseacae, such as apple and pear. It provokes necrosis in aerial parts of susceptible host plants and the typical hypersensitive reaction in non-host plants. The secreted harpin, HrpN ea, is able by itself to induce an active cell death in non-host plants. Ion flux modulations were shown to be involved early in such processes but very few data are available on the plasma membrane ion channel activities responsible for the pathogen-induced ion fluxes. We show here that HrpN ea induces cell death in non-host Arabidopsis thaliana suspension cells. We further show that two cystic fibrosis transmembrane conductance regulator modulators, glibenclamide and bromotetramisole, can regulate anion channel activities and HrpN ea-induced cell death.
Reboutier
David
D
LEM, EA 3514, Université Paris 7, Case 7069, 2, place Jussieu, 75251 Paris cedex 5, France. reboutie@ccr.jussieu.fr
Frankart
Cécile
C
Vedel
Régine
R
Brault
Mathias
M
Duggleby
Ronald G
RG
Rona
Jean Pierre
JP
Barny
Marie Anne
MA
Bouteau
François
F
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000494:pharmacology; D018929:Cell Culture Techniques; D016923:Cell Death; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000819:agonists*; D044084:Erwinia amylovora / Q000378:metabolism*; D005905:Glyburide / Q000494:pharmacology; D015640:Ion Channel Gating; D013773:Tetramisole / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; C075462:harpin protein, Erwinia amylovora; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D013773:Tetramisole; D005905:Glyburide