title: Functional studies of mesenchymal stem cells derived from adult human adipose tissue.
abstract: Recent evidence suggests that cells with the properties of human mesenchymal stem cells (hMSCs) can be derived from adult peripheral tissues, including adipose tissue, muscle and dermis. We isolated hMSCs from the stromal-vascular portion of subcutaneous adipose tissue from seven adult subjects. These cells could be readily differentiated into cells of the chondrocyte, osteocyte and adipocyte lineage demonstrating their multipotency. We studied the functional properties of hMSCs-derived adipocytes and compared them with adipocytes differentiated from hMSCs obtained from bone marrow (BM-hMSC). The two cell types displayed similar lipolytic capacity upon stimulation with catecholamines, including a pronounced antilipolytic effect mediated through alpha2A-adrenoceptors, a typical trait in human but not rodent fat cells. Furthermore, both cell types secreted the fat cell-specific factors leptin and adiponectin in comparable amounts per time unit. The fat tissue-derived hMSCs retained their differentiation capacity up to at least fifteen passages. We conclude that hMSCs derived from adult human adipose tissue can be differentiated into fully functional adipocytes with a similar, if not identical, phenotype as that observed in cells derived from BM-hMSCs. Human adipose-tissue-derived MSCs could therefore constitute an efficient and easily obtainable renewable cellular source for studies of adipocyte biology.
authors.affiliation: Department of Medicine, M63, Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
authors.forename: Andrea
authors.initials: A
authors.lastname: Dicker
authors.forename: Katarina
authors.initials: K
authors.lastname: Le Blanc
authors.forename: Gaby
authors.initials: G
authors.lastname: Aström
authors.forename: Vanessa
authors.initials: V
authors.lastname: van Harmelen
authors.forename: Cecilia
authors.initials: C
authors.lastname: Götherström
authors.forename: Lennart
authors.initials: L
authors.lastname: Blomqvist
authors.forename: Peter
authors.initials: P
authors.lastname: Arner
authors.forename: Mikael
authors.initials: M
authors.lastname: Rydén
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D052242:Adiponectin; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism*; D058647:Adrenergic alpha-2 Receptor Agonists; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism / Q000494:pharmacology; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D010641:Phenotype; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism
chemical_list: D052242:Adiponectin; D058647:Adrenergic alpha-2 Receptor Agonists; D002395:Catecholamines; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D018341:Receptors, Adrenergic, alpha-2