Gene expression profile of amyloid beta protein-injected mouse model for Alzheimer disease.
OBJECTIVE
To investigate the gene expression profile changes in the cerebral cortex of mice injected icv with amyloid beta-protein (Abeta) fragment 25-35 using cDNA microarray.


METHODS
Balb/c mice were randomly divided into a control group and Abeta-treated group. The Morris water maze test was performed to detect the effect of Abeta-injection on the learning and memory of mice. Atlas Mouse 1.2 Expression Arrays containing 1176 genes were used to investigate the gene expression pattern of each group.


RESULTS
The gene expression profiles showed that 19 genes including TBX1, NF-kB, AP-1/c-Jun, cadherin, integrin, erb-B2, and FGFR1 were up-regulated after 2 weeks of icv administration of Abeta; while 12 genes were down-regulated, including NGF, glucose phosphate isomerase 1, AT motif binding factor 1, Na+/K+-ATPase, and Akt.


CONCLUSIONS
The results provide important leads for pursuing a more complete understanding of the molecular events of Abeta-injection into mice with Alzheimer disease.
Kong
Ling-na
LN
Department of Pharmacology, School of Basic Medicine, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100005, China.
Zuo
Ping-ping
PP
Mu
Liang
L
Liu
Yan-yong
YY
Yang
Nan
N
D000544:Alzheimer Disease / Q000139:chemically induced / Q000235:genetics*; D016229:Amyloid beta-Peptides; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D020869:Gene Expression Profiling; D008297:Male; D018782:Maze Learning; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010446:Peptide Fragments; D020825:T-Box Domain Proteins / Q000378:metabolism
D016229:Amyloid beta-Peptides; D016328:NF-kappa B; D010446:Peptide Fragments; D020825:T-Box Domain Proteins; C108625:TBX1 protein, human; C074636:amyloid beta-protein (25-35); D020932:Nerve Growth Factor