title: Deletion of the ferric uptake regulator Fur impairs the in vitro growth and virulence of Actinobacillus pleuropneumoniae.
abstract: In order to investigate the role of the ferric uptake regulator Fur in the porcine lung pathogen Actinobacillus pleuropneumoniae, we constructed an isogenic in-frame deletion mutant, A. pleuropneumoniae Deltafur. This mutant showed constitutive expression of transferrin-binding proteins, growth deficiencies in vitro, and reduced virulence in an aerosol infection model.
authors.affiliation: Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Ilse
authors.initials: I
authors.lastname: Jacobsen
authors.forename: Jörg
authors.initials: J
authors.lastname: Gerstenberger
authors.forename: Achim D
authors.initials: AD
authors.lastname: Gruber
authors.forename: Janine T
authors.initials: JT
authors.lastname: Bossé
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langford
authors.forename: Isabel
authors.initials: I
authors.lastname: Hennig-Pauka
authors.forename: Jochen
authors.initials: J
authors.lastname: Meens
authors.forename: Gerald-F
authors.initials: GF
authors.lastname: Gerlach
mesh_terms: D000189:Actinobacillus Infections / Q000378:metabolism / Q000473:pathology; D016977:Actinobacillus pleuropneumoniae / Q000254:growth & development* / Q000472:pathogenicity; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D007501:Iron / Q000378:metabolism; D008168:Lung / Q000473:pathology; D012097:Repressor Proteins / Q000502:physiology*; D013552:Swine; D033882:Transferrin-Binding Protein B / Q000235:genetics; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D033882:Transferrin-Binding Protein B; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron