Bryostatin-1 in combination with calcium ionophore promotes the maturation of human umbilical cord-blood monocyte-derived dendritic cells capable of activating neonatal alloreactive T cells.
We have investigated the effect of bryostatin-1 (Bryo-1) and calcium ionophore (CI) on maturation and functions of DCs generated from adherent cells of cord blood cultured with GM-CSF plus IL-4 (CB-DCs). The CB-DCs treated with Bryo-1+CI exhibited morphologic characteristics of mature DCs, expressed increased levels of CD1a, CD80, CD83, CD86, and MHC class II as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN-gamma production. Treatment of CB-DCs with TNF-alpha or PMA+CI was less effective. Thus, Bryo-1+CI promotes maturation of CB-DCs and therefore could be used to enhance the neonatal immune response.
Do
Yoonkyung
Y
Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298-0678, USA.
Mainali
Elsie
E
Nagarkatti
Prakash S
PS
Nagarkatti
Mitzi
M
D054713:Bryostatins; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000096:biosynthesis; D007476:Ionophores / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D018942:Macrolides / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D054713:Bryostatins; D007476:Ionophores; D018942:Macrolides; C046785:bryostatin 1; D007371:Interferon-gamma; D002118:Calcium