Effects of active fractions from Lycopus lucidus L. F04 on platelet aggregation and thrombus formation.
OBJECTIVE
To study the effect of active fractions from Lycopus lucidus (L. F04) on platelet aggregation and thrombus formation so as to investigate its mechanism of promoting blood circulation and removing blood stasis.


METHODS
The effects of Lycopus lucidus L. F04 on platelet aggregation induced by ADP in vivo, and thrombosis of artery-vein by-pass and thrombus formed in circuitous loop in vitro were examined using the blood stasis animal model of rats made by injecting high molecular weight dextran (HMWD).


RESULTS
The increase of maximum platelet aggregation rate induced by ADP in vivo in HMWD model was evidently inhibited with 0.408 g/kg or 0.204 g/kg or L. F04, and the effect showed a trend of concentration-dependence. As compared with the control, the thrombus weight in rat model of blood stasis increased clearly and its length showed only a trend of increase. Both 0.408 g/kg and 0.204 g/kg of L. F04 had the effect of resisting the thrombus formation, while 0.408 g/kg showed better effects of reducing the thrombus dry weight and wet weight. Both 0.408 g/kg and 0.204 g/kg of L. F04 could inhibit the thrombosis in artery-vein by-pass; the inhibition rates were 27.41% and 27.14% respectively.


CONCLUSIONS
Lycopus lucidus L. F04 could significantly inhibit platelet aggregation and thrombus formation.
Shi
Hong-zhi
HZ
Institute of Space Medico-Engineering, Beijing. shihongz2002@yahoo.com.cn
Gao
Nan-nan
NN
Li
Yong-zhi
YZ
Yu
Jing-guang
JG
Fan
Quan-chun
QC
Bai
Gui-e
GE
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D003911:Dextrans / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D031333:Lycopus; D008297:Male; D008517:Phytotherapy; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000188:drug therapy
D000925:Anticoagulants; D003911:Dextrans; D004365:Drugs, Chinese Herbal