Within the cell: analytical techniques for subcellular analysis.
This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.
Olson
Karen J
KJ
Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, MN 55455, USA.
Ahmadzadeh
Hossein
H
Arriaga
Edgar A
EA
D004586:Electrophoresis / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D015388:Organelles / Q000737:chemistry; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions / Q000737:chemistry*