A test for ectopic exchange catalyzed by Cre recombinase in maize.
A maize line expressing Cre recombinase as well as the recipient line without the transgene were assayed for evidence of ectopic recombination within the maize genome. Such a test is valuable for understanding the action of Cre as well as for its use to recombine two target lox sites present in the chromosomes. Pollen examination and seed set tests of material expressing Cre provided no evidence of ectopic recombination, which would be manifested in the production of translocations or inversions and result in pollen abortion and reduced seed set. Root-tip chromosome karyotype analysis was also performed on material with and without Cre expression. Chromosomal aberrations in Cre+ material were not observed above the background level.
Ream
Thomas S
TS
Division of Biological Sciences, University of Missouri-Columbia, Columbia, 65211, USA.
Strobel
Jonathan
J
Roller
Brandon
B
Auger
Donald L
DL
Kato
Akio
A
Halbrook
Cynthia
C
Peters
Eric M
EM
Theuri
James
J
Bauer
Matthew J
MJ
Addae
Prince
P
Dioh
Waly
W
Staub
Jeffrey M
JM
Gilbertson
Larry A
LA
Birchler
James A
JA
D002869:Chromosome Aberrations; D017404:In Situ Hybridization, Fluorescence; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007621:Karyotyping; D016296:Mutagenesis / Q000235:genetics; D011058:Pollen / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases