title: A new reversion assay for measuring all possible base pair substitutions in Saccharomyces cerevisiae.
abstract: A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.
authors.affiliation: Department of Biology, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: Teresa-Marie
authors.initials: TM
authors.lastname: Williams
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Fabbri
authors.forename: Jason W
authors.initials: JW
authors.lastname: Reeves
authors.forename: Gray F
authors.initials: GF
authors.lastname: Crouse
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment