Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells.
The intracellular bacterium Chlamydia pneumoniae is involved in the inflammation process of atherosclerosis. We previously demonstrated that C. pneumonia infected monocytes (THP-1 cells) responded to stimulation by an increased respiratory burst linked to an increased NADPH oxidase (NOX) activity. We now tested agents acting on the assembly of the NOX subunits or on protein kinase C, a trigger of NOX activity. Apocynin, resveratrol, rutin, quercetin, curcumin, and tocopherols were tested. The cells were pre-incubated with Chlamydia and the agent for 19 h, and then stimulated with phorbol myristate acetate. The NOX activity was monitored by measuring the hydrogen peroxide production. Resveratrol and curcumin (10(-4)-10(-6) M) were better inhibitors than apocynin. alpha-Tocopherol was inactive, and gamma-tocopherol inhibitor at 10(-4) M only. Quercetin was inactive, and rutin a moderate but significant inhibitor. The inhibition by resveratrol was increased by 10(-6) M rutin or quercetin. Resveratrol and curcumin thus appeared to be interesting for atherosclerosis treatment.
Deby-Dupont
Ginette
G
Center for Oxygen, Research and Development (CORD), Institut de Chimie, B6a, University of Liège, Sart Tilman, Belgium.
Mouithys-Mickalad
Ange
A
Serteyn
Didier
D
Lamy
Maurice
M
Deby
Carol
C
D000975:Antioxidants / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D003474:Curcumin / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors*; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*
D000975:Antioxidants; D013267:Stilbenes; D019255:NADPH Oxidases; D003474:Curcumin; D000077185:Resveratrol