Comparison of autologous cell therapy and granulocyte-colony stimulating factor (G-CSF) injection vs. G-CSF injection alone for the treatment of acute radiation syndrome in a non-human primate model.
OBJECTIVE
To compare the efficacy of autologous cell therapy after irradiation combined with granulocyte-colony stimulating factor (G-CSF) injections with G-CSF treatment alone in a heterogeneous model of irradiation representative of an accidental situation.


METHODS
Non-human primates were irradiated at 8.7 Gy whole-body dose with the right arm shielded to receive 4.8 Gy. The first group of animals received G-CSF (lenograstim) injections starting 6 h after irradiation, and a second group received a combination of G-CSF (lenograstim) injections and autologous expanded hematopoietic cells. Animals were followed up for blood cell counts, circulating progenitors, and bone marrow cellularity.


RESULTS
No significant differences were seen between the two treatment groups, whatever the parameter observed: time to leukocyte or platelet recovery and duration and severity of aplasia.


CONCLUSIONS
Our results indicated that identical recovery kinetic was observed when irradiated animals are treated with G-CSF independently of the reinjection of ex vivo expanded autologous hematopoietic cells. Thus G-CSF injections might be chosen as a first-line therapeutic strategy in the treatment of accidental acute radiation victims.
Bertho
Jean-Marc
JM
Institut de Radioprotection et de Sûreté Nucléaire, Service de Radiobiologie et d'Epidémiologie, Laboratoire de Thérapie Cellulaire et de Radioprotection Accidentelle, Fontenay-aux-Roses, France. jean-marc.bertho@irsn.fr
Frick
Johanna
J
Prat
Marie
M
Demarquay
Christelle
C
Dudoignon
Nicolas
N
Trompier
François
F
Gorin
Norbert-Claude
NC
Thierry
Dominique
D
Gourmelon
Patrick
P
D000818:Animals; D003131:Combined Modality Therapy; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006410:Hematopoiesis / Q000528:radiation effects*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D000078224:Lenograstim; D008252:Macaca fascicularis; D008297:Male; D011833:Radiation Injuries, Experimental / Q000628:therapy*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013577:Syndrome; D016896:Treatment Outcome
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000078224:Lenograstim