A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana.
Banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. The disease is caused by Banana bunchy top virus (BBTV), a multicomponent, single-stranded DNA virus of the family Nanoviridae. We have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase chain reaction (PCR)-mediated detection of BBTV. In addition, primers to banana genomic sequence are used as an internal control, overcoming the uncertainty (owing to false-negatives) inherent in PCR diagnostics. Together these primer sets are a valuable tool in the effort to control BBTV, particularly in screening micropropagated banana plantlets for the absence of virus before release to farmers.
Mansoor
Shahid
S
National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
Qazi
Javaria
J
Amin
Imran
I
Khatri
Abdullah
A
Khan
Imtiaz A
IA
Raza
Saboohi
S
Zafar
Yusuf
Y
Briddon
Rob W
RW
D004279:DNA, Viral / Q000032:analysis; D028521:Musa / Q000821:virology*; D025682:Nanovirus / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral