title: Endovascular needle injection of cholesteryl linoleate into the arterial wall produces complex vascular lesions identifiable by intravascular ultrasound: early development in a porcine model of vulnerable plaque.
abstract: BACKGROUND
  We attempted to create a pig model of complex arterial lesions through the percutaneous injection of cholesteryl linoleate into the vessel wall.
  
  
  RESULTS
  A total of 81 arterial segments (27 arteries) underwent percutaneous intramural injection of cholesteryl linoleate, in eight pigs. Intravascular ultrasound (IVUS) analysis and corresponding histology were obtained for analysis at 2 and 4 weeks after injection. Overall, 18 out of 27 (67%) of the injected arterial segments displayed lesions identifiable by IVUS as an eccentric echolucent zone present within the deeper layer of the lesion. Quantitative IVUS analysis demonstrated that these lesions were non-occlusive (36+/-8% area stenosis), eccentric (eccentricity index, 0.78+/-0.07) and located into positively remodeled vessels (remodeling index, 1.45+/-0.24). By histology, these lesions were eccentric and comprised less than a third of the vessel circumference. Medial thickening and a thickened intima containing lipid droplets and mononuclear cells were consistently found. The presence of lipids or local wall thickening seen by histology colocalized with the presence of echolucent structures seen by IVUS in 65% of the coronary segments and 70% of the iliac segments.
  
  
  CONCLUSIONS
  The intramural deposition of cholesteryl linoleate results in the development of complex, lipid-containing inflammatory lesions in less than 4 weeks. These lesions are already identifiable by IVUS at 2 weeks and colocalize with histologic findings. Further development of this model may allow the validation of technologies designed to detect and treat high-risk atherosclerotic lesions.
authors.affiliation: Cardiovascular Intervention Core, Methodist Hospital Research Institute, Houston, Texas 77030, USA.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Granada
authors.forename: Pedro R
authors.initials: PR
authors.lastname: Moreno
authors.forename: Allen P
authors.initials: AP
authors.lastname: Burke
authors.forename: Daryl G
authors.initials: DG
authors.lastname: Schulz
authors.forename: Albert E
authors.initials: AE
authors.lastname: Raizner
authors.forename: Greg L
authors.initials: GL
authors.lastname: Kaluza
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000000981:diagnostic imaging* / Q000473:pathology*; D001707:Biopsy, Needle; D002788:Cholesterol Esters; D003331:Coronary Vessels / Q000000981:diagnostic imaging / Q000473:pathology*; D004195:Disease Models, Animal; D007083:Iliac Artery / Q000000981:diagnostic imaging / Q000473:pathology*; D007150:Immunohistochemistry; D007269:Injections, Intra-Arterial; D011336:Probability; D012680:Sensitivity and Specificity; D034421:Sus scrofa; D017539:Tunica Intima / Q000000981:diagnostic imaging / Q000473:pathology; D018084:Ultrasonography, Interventional
chemical_list: D002788:Cholesterol Esters; C021976:cholesteryl linoleate