In situ generation of Co(II) by use of a solid-phase reactor in an FIA assembly for the spectrophotometric determination of penicillamine.
A flow injection analysis (FIA) manifold for the determination of penicillamine in pharmaceutical preparations is proposed. The manifold includes a solid-phase reactor for the in situ production of the derivatizing reagent, Co(II) ion, which forms a coloured complex with penicillamine in an alkaline medium. The reactor is prepared by natural immobilization of cobalt carbonate on a polymer matrix, which endows it with a high mechanical and microbiological stability. The cobalt released by passage of a 5 x 10(-4) mol l(-1) sulphuric acid stream at a flow-rate of 2.3 ml min(-1) is merged with a volume of 314 microl of sample containing penicillamine in ammonium-ammonia buffer at pH 9.5 to measure the absorbance at 360 nm. Beer's law is obeyed over the penicillamine concentration range 5-60 mg l(-1). The limit of detection (LOD) of the method is 1 mg l(-1) and its throughput 70 samples h(-1).
Corominas
B Gómez-Taylor
BG
Departamento de Química, Bioquímica y Biología Molecular, Universidad Cardenal Herrera CEU, Moncada, Valencia, Spain.
Pferzschner
Julia
J
Icardo
M Catalá
MC
Zamora
L Lahuerta
LL
Martínez Calatayud
J
J
D003035:Cobalt / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation*; D010396:Penicillamine / Q000032:analysis*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
D003035:Cobalt; D010396:Penicillamine