title: [L-fucose-specific lectin from pike perch (Lucioperca lucioperca L.) roe. Purification and studies of carbohydrate specificity].
abstract: L-fucose-specific lectin was purified from pike perch (Lucioperca lucioperca L.) roe by affinity chromatography on ovariomucine H-sepharose from a human ovary cyst. Three bands were detected after disk-electrophoresis in PAAG in alkaline (pH 8.9) and five bands--in acidic system (pH 4.3). According to electrophoresis data in 15% SDS-PAGE the lectin contains two components with molecular weight 13-14 kDa. Molecular weight of the lectin is 50 kDa according to gel-chromatography on tojopearl HW-55. The immunochemical properties of the lectins from perch (Persa fluviatilis L.) roe and pike perch (Lucioperca lucioperca L.) roe are similar.
authors.forename: V O
authors.initials: VO
authors.lastname: Antoniuk
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010063:Ovum / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D002241:Carbohydrates; D037102:Lectins; C027112:fucose-binding lectin