The 5' region of the human hSUV3 gene encoding mitochondrial DNA and RNA helicase: promoter characterization and alternative pre-mRNA splicing.
The human nuclear hSUV3 gene encodes ATP-dependent RNA and DNA helicase, which predominantly localizes in the mitochondria. In yeast, the Suv3 helicase is a component of mitochondrial degradosome, a two-subunit complex, which degrades aberrant mtRNAs. In contrast to the well-documented physiological role of the yeast SUV3, the function of its human orthologue remains unknown. In this report, we have analyzed the hSUV3 5' genomic region. Our data suggest that hSUV3 is a housekeeping gene. Deletion analysis and in vitro mutagenesis revealed the presence of an enhancer region and regulatory elements in basal promoter including: (i) direct 10-bp-long repeats, which share significant sequence similarity with the consensus for the NF-kappaB/Rel family transcription factors, (ii) Sp1 general transcription factor binding site, and (iii) NRF-1 transcription factor binding sites, the latter typical for nuclear-encoded mitochondrial genes. Furthermore, we show that the 5' region of the hSUV3 pre-mRNA can be alternatively spliced.
Minczuk
Michal
M
Department of Genetics, University of Warsaw, Pawinskiego 5A, 02-106 Warsaw, Poland. mminczuk@ibb.waw.pl
Lilpop
Joanna
J
Boros
Joanna
J
Stepien
Piotr P
PP
D024506:5' Flanking Region; D017398:Alternative Splicing; D001483:Base Sequence; D001665:Binding Sites; D018899:CpG Islands; D053487:DEAD-box RNA Helicases; D004742:Enhancer Elements, Genetic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020365:RNA Helicases / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D024363:Transcription Initiation Site
D016329:Sp1 Transcription Factor; C469673:SUPV3L1 protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases