[Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district].
OBJECTIVE
To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.


METHODS
Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package.


RESULTS
A 278 base pairs region of the gene for CO I encoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species.


CONCLUSIONS
It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the CO I encoding gene of mtDNA.
Cai
Ji-feng
JF
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. cjf_jifeng@163.com
Dong
Jian-guo
JG
Liu
Min
M
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods; D007814:Larva / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011679:Pupa / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV