Gene expression profiling to characterize anticancer drug sensitivity.
This chapter presents a protocol for using cDNA microarrays to acquire gene expression profiles that characterize anticancer drug sensitivity. The protocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data processing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommended.
Breaux
James K
JK
Rebecca and John Moores UCSD Cancer Center, University of California-San Diego, La Jolla, USA.
Los
Gerrit
G
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000032:analysis / Q000187:drug effects*; D013223:Statistics as Topic; D013997:Time Factors; D043922:Toxicogenetics
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012334:RNA, Neoplasm