title: Cooperative folding in a multi-domain protein.
abstract: Most protein domains are found in multi-domain proteins, yet most studies of protein folding have concentrated on small, single-domain proteins or on isolated domains from larger proteins. Spectrin domains are small (106 amino acid residues), independently folding domains consisting of three long alpha-helices. They are found in multi-domain proteins with a number of spectrin domains in tandem array. Structural studies have shown that in these arrays the last helix of one domain forms a continuous helix with the first helix of the following domain. It has been demonstrated that a number of spectrin domains are stabilised by their neighbours. Here we investigate the molecular basis for cooperativity between adjacent spectrin domains 16 and 17 from chicken brain alpha-spectrin (R16 and R17). We show that whereas the proteins unfold as a single cooperative unit at 25 degrees C, cooperativity is lost at higher temperatures and in the presence of stabilising salts. Mutations in the linker region also cause the cooperativity to be lost. However, the cooperativity does not rely on specific interactions in the linker region alone. Most mutations in the R17 domain cause a decrease in cooperativity, whereas proteins with mutations in the R16 domain still fold cooperatively. We propose a mechanism for this behaviour.
authors.affiliation: University of Cambridge, Department of Chemistry, MRC Centre for Protein Engineering, Lensfield Rd, Cambridge CB2 1EW, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Batey
authors.forename: Lucy G
authors.initials: LG
authors.lastname: Randles
authors.forename: Annette
authors.initials: A
authors.lastname: Steward
authors.forename: Jane
authors.initials: J
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012492:Salts; D012996:Solutions; D013049:Spectrin / Q000737:chemistry* / Q000235:genetics; D013816:Thermodynamics
chemical_list: D011994:Recombinant Proteins; D012492:Salts; D012996:Solutions; D013049:Spectrin