[Fabrication of decellularized scaffold of homograft bioprosthetic tube valved with two kinds of cell detergents].
OBJECTIVE
To compare the effect of fabricating decellularized scaffold of homograft bioprosthetic tube valved (HBTV) with two kinds of cell detergents and to provide a homograft bioprosthetic scaffold for fabrication of tissue-engineering heart valve (TEHV).


METHODS
The active cells in the HBTV, which conserved by liquid nitrogen, were decellularized by low osmotic pressure of Tris buffer, in which containing sodium dodecylsulphate (SDS) and deoxycholic acid (DOA) respectively. The leaflets or aortic wall was fixed with fixative and stained with hematoxylin and eosin, collagen fibers or elastic fibers for observation and photographs by light microscope or by scanning electron microscope (SEM) after decellularized.


RESULTS
When the leaflets of HBTV were incubated together with 0.03% SDS or 0.5% DOA of Tris buffer respectively for 48 hours, the active endothelial cells (ECs) in the leaflets were not only decellularized completely, but also reserved the collagen fibers or elastic fibers integrally, which is two of the main components of extracellular matrix (ECM). A part of fibroblast in the center leaflets was reserved. The morphologic structure of leaflets after decellularized was not significantly different from that before decellularized. The concentration of SDS was increased to 0.1% when decellularized the cells of aortic wall, but DOA was still kept 0.5%.


CONCLUSIONS
The better decellularized scaffold of HBTV obtained was disposed by 0.03%-0.1% SDS or 0.5% DOA, which was advantageous to adhesiveness and amplification of implantation cells on the decellularized scaffold of HBTV in order that HBV re-endothelialized or for the TEHV fabricated in vitro.
Feng
Bin
B
Department of Cardiac Surgery, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Beijing Union Medical College, Beijing, 100037, PR China. fsn9977@yahoo.com.cn
Lui
Yinglong
Y
Xie
Nin
N
D001705:Bioprosthesis; D003840:Deoxycholic Acid / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000166:cytology / Q000187:drug effects; D006801:Humans; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous
D003902:Detergents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate