[SNP genotyping by multiplex amplification and microarrays assay and forensic application].
OBJECTIVE
Research on the application feasibility of SNP genotyping for forensic identification by microarrays.


METHODS
Oligonucleotide microarrays which could detect 31 different SNPs were used. Population studies on 31 SNP loci was carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.


RESULTS
No deviations from Hardy-Weinberg equilibrium could be found at the 31 SNP loci 4 loci were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal informative SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in duos and trios were 0.9609 and 0.9970 respectively.


CONCLUSIONS
The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification.
Li
Li
L
Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, China.
Li
Rong-yu
RY
Li
Cheng-tao
CT
D044466:Asian People / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005554:Forensic Medicine / Q000379:methods; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010334:Paternity; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D020080:Tandem Repeat Sequences
D017931:DNA Primers