Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical systems and species-specific PCR.
One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with "low discrimination" whereas the VITEK2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.
Giordano
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A
Dipartimento di Scienze di Sanità Pubblica Sezione di Microbiologia Università degli Studi di Roma La Sapienza, Via M. Fascetti, 67, 00136 Rome, Italy. alessandro.giordano@uniroma1.it
Magni
A
A
Trancassini
M
M
Varesi
P
P
Turner
R
R
Mancini
C
C
D001431:Bacteriological Techniques / Q000379:methods*; D001980:Bronchi / Q000382:microbiology; D003550:Cystic Fibrosis / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D013183:Sputum / Q000382:microbiology; D020615:Stenotrophomonas maltophilia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014132:Trachea / Q000382:microbiology