Two polyene amides produced by genetically modified Streptomyces diastaticus var. 108.
Streptomyces diastaticus var. 108, a newly isolated strain, was recently characterized as a producer of two polyene macrolide antibiotics (rimocidin and CE-108), and the biosynthetic gene cluster was partially characterized. When the producer strain was genetically modified by transformation with some engineered SCP2*-derived vectors carrying the ermE gene, two previously uncharacterized macrolides were detected in the fermentation broth of the recombinant strain and chemically characterized as the amides of the parental polyene carboxylic acids. The biological activity and some in vitro toxicity assays showed that this chemical modification resulted in pharmaceuticals with improved biological properties compared with the parental products.
Seco
Elena M
EM
Centro Nacional de Biotecnología, Campus de la UAM, 28049 Cantoblanco, Madrid, Spain.
Cuesta
Trinidad
T
Fotso
Serge
S
Laatsch
Hartmut
H
Malpartida
Francisco
F
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D005658:Fungi / Q000187:drug effects; D005798:Genes, Bacterial; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D018942:Macrolides / Q000302:isolation & purification* / Q000494:pharmacology; D008780:Methyltransferases / Q000235:genetics; D009005:Monosaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D011090:Polyenes / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D000935:Antifungal Agents; C485858:CE-108; D018942:Macrolides; D009005:Monosaccharides; D011090:Polyenes; C003273:rimocidin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase