title: Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
abstract: In vitro infection of bovine cells of many origins with the cytopathogenic bovine viral diarrhea virus (cpBVDV) results in the induction of alpha/beta interferon (IFN-alpha/beta), whereas noncytopathogenic BVDV (ncpBVDV) isolates have been shown not to induce IFN-alpha/beta in vitro. Similarly, cpBVDV induces IFN-alpha/beta in the early bovine fetus, but ncpBVDV does not. However, acute infection of naive cattle with ncpBVDV results in IFN-alpha/beta production. In this study, we identified and characterized a minor population of cells, present in lymph nodes that produce IFN-alpha in response to ncpBVDV. These cells expressed the myeloid markers CD14, CD11b, and CD172a but did not express CD4 and CD45RB. We also established that these cells produced IFN-alpha in the absence of detectable productive infection.
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. bryan.charleston@bbsrc.ac.uk.
authors.forename: L S
authors.initials: LS
authors.lastname: Brackenbury
authors.forename: B V
authors.initials: BV
authors.lastname: Carr
authors.forename: Z
authors.initials: Z
authors.lastname: Stamataki
authors.forename: H
authors.initials: H
authors.lastname: Prentice
authors.forename: E A
authors.initials: EA
authors.lastname: Lefevre
authors.forename: C J
authors.initials: CJ
authors.lastname: Howard
authors.forename: B
authors.initials: B
authors.lastname: Charleston
mesh_terms: D000818:Animals; D015703:Antigens, CD; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000821:virology; D039481:CD11b Antigen / Q000378:metabolism; D002417:Cattle; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000472:pathogenicity; D016898:Interferon-alpha / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; D016898:Interferon-alpha; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D016899:Interferon-beta