[Changes in the composition of anionic membrane phospholipids influence the protein secretion and biogenesis of cell envelope in Escherichia coli].
The secretion of alkaline phosphatase (PhoA) and peculiarities of biogenesis of the cell envelope were studied in Escherichia coli strains HD30/pHD 102 and HDL11 with controlled synthesis of anionic phospholipids, phosphatidylglycerol, and cardiolipin. Inactivation of the pgsA gene encoding the synthesis of anionic phospholipids or changes in the regulation of its expression by an environmental factor caused changes in the metabolism and composition of membrane phospholipids, which resulted in a decrease in the secretion of alkaline phosphatase through the cytoplasmic membrane and an increase in PhoA secretion from the periplasm into the culture medium. A conforming increase was observed in exopolysaccharide secretion, as well as a decrease in the contents of lipopolysaccharide and lipopolyprotein of the outer membrane that determine the membrane barrier properties. The results obtained testify that anionic phospholipids play a significant role in protein secretion and are probably involved in the interrelation between the protein secretion and biogenesis of cell envelope components.
Anisimova
E V
EV
Badiakina
A O
AO
Vasil'eva
N V
NV
Nesmeianova
M A
MA
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D002308:Cardiolipins / Q000096:biosynthesis; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D019897:Periplasm / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000096:biosynthesis; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D017354:Point Mutation; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D002308:Cardiolipins; D029968:Escherichia coli Proteins; D010715:Phosphatidylglycerols; D010743:Phospholipids; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; D000469:Alkaline Phosphatase