Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation.
BACKGROUND
The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis.


METHODS
A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores.


RESULTS
The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals.


CONCLUSIONS
This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.
Maddalo
Gianluca
G
Centro Studi sull'Invecchiamento (Ce.S.I.), Fondazione Università "G.D'Annunzio", Chieti, Italy.
Petrucci
Francesca
F
Iezzi
Manuela
M
Pannellini
Tania
T
Del Boccio
Piero
P
Ciavardelli
Domenico
D
Biroccio
Antonino
A
Forlì
Federica
F
Di Ilio
Carmine
C
Ballone
Enzo
E
Urbani
Andrea
A
Federici
Giorgio
G
D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D016014:Linear Models; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000378:metabolism; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012468:Salivary Gland Neoplasms / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D020543:Proteome