Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser-induced native fluorescence detection.
This paper describes the analysis of biologically active amines by capillary electrophoresis (CE) in conjunction with laser-induced native fluorescence detection. In order to simultaneously analyze amines and acids as well as to achieve high sensitivity, 10 mM formic acid solutions (pH < 4.0) containing silica nanoparticles (SiNPs) were chosen as the background electrolytes. With increasing SiNP concentration, the migration times for seven analytes decrease as a result of increase in electroosmotic flow (EOF) and decrease in their electrophoretic mobilities against EOF. A small EOF generated at pH 3.0 reveals adsorption of SiNPs on the deactivated capillary wall. The decreases in electrophoretic mobilities with increasing SiNP concentration up to 0.3x indicate the interactions between the analytes and the SiNPs. Having a great sensitivity (the limits of detection at a signal-to-noise ratio (S/N) = 3 of 0.09 nM for tryptamine (TA)), high efficiency, and excellent reproducibility (less than 2.4% of the migration times), this developed method has been applied to the analysis of urinal samples with the concentrations of 0.50 +/- 0.02 microM, 0.49 +/- 0.04 microM, and 74 +/- 2 microM for TA, 5-hydroxytryptamine, and tryptophan, respectively. The successful examples demonstrated in this study open up a possibility of using functional nanoparticles for the separation of different analytes by CE.
Kuo
I-Ting
IT
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Huang
Yu-Fen
YF
Chang
Huan-Tsung
HT
D001826:Body Fluids / Q000737:chemistry; D002395:Catecholamines / Q000302:isolation & purification* / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007834:Lasers; D049329:Nanostructures; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D012822:Silicon Dioxide