title: Identification and characterization of the afsR homologue regulatory gene from Streptomyces peucetius ATCC 27952.
abstract: We have isolated an afsR homologue, called afsR-p, through genome analysis of Streptomyces peucetius ATCC 27952. AfsR-p shares 60% sequence identity with AfsR from Streptomyces coelicolor A3 (2). afsR-p was expressed under the control of the ermE* promoter in its hosts S. peucetius, Streptomyces lividans TK 24, Streptomyces clavuligerus and Streptomyces griseus. We observed overproduction of doxorubicin (4-fold) in S. peucetius, gamma-actinorhodin (2.6-fold) in S. lividans, clavulanic acid (1.5-fold) in S. clavuligerus and streptomycin (slight) in S. griseus. Overproduction was due to expression of the gene in these strains as compared to the wild-type strains harboring the vector only. Comparative study of the expression of afsR-p revealed that regulatory networking in Streptomyces is not uniform. We speculate that phosphorylated AfsR-p becomes bound to the promoter region of afsS. The latter activates other regulatory genes, including pathway regulatory genes, and induces the production of secondary metabolites including antibiotics. We identified specific conserved amino acids and exploited them for the isolation of the partial sequence of the afsR homologue from S. clavuligerus and Streptomyces achromogens (rubradirin producer). Such findings provide additional evidence for the presence of a serine/threonine and tyrosine kinase-dependent global regulatory network in Streptomyces.
authors.affiliation: Institute of Biomolecule Reconstruction, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asansi, Chungnam 336-708, Republic of Korea.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Parajuli
authors.forename: Hung Trinh
authors.initials: HT
authors.lastname: Viet
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishida
authors.forename: Hang Thi
authors.initials: HT
authors.lastname: Tong
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics; D019818:Clavulanic Acid / Q000032:analysis; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004317:Doxorubicin / Q000032:analysis; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D007783:Lactones / Q000032:analysis; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D013307:Streptomycin / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D001426:Bacterial Proteins; D019818:Clavulanic Acid; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D004317:Doxorubicin; D007783:Lactones; D008780:Methyltransferases; D013307:Streptomycin; D014157:Transcription Factors; C098846:gamma-actinorhodin; D000095622:Benzoisochromanequinones; C035181:rRNA (adenosine-O-2'-)methyltransferase