[Application of McAbs against rabies nucleocapsid in diagnosis of rabies street virus].
OBJECTIVE
McAbs against rabies nucleocapsid were used to detect rabies street viruses in animal brain specimens with indirect immunofluorescent assay to evaluate the sensitivity and specificity of this assay.


METHODS
62 specimen from rabid animal brains including genotype 1 to 7 and 271 specimens from different normal animal brains collected in Pasteur Institute in 2003 were tested and compared, using indirect immunofluorescent assay. All these specimens were identified and compared using rapid rabies enzyme immunodiagnosis, fluorescent antibody test and rabies virus isolation assay in neuroblastoma cell culture which were all provided by Pasteur Institute.


RESULTS
Both sensitivity and the specificity of the indirect immunofluorescent assay were 100%.


CONCLUSIONS
The results showed a positive of rabies virus detection with these methods.
Xu
Ge-lin
GL
Wuhan Institute of Biological Product, Wuhan 430060, China.
Yan
Jia-xin
JX
Larrous
F
F
Zhu
Yu-tao
YT
Cozette
P
P
Xue
Hong-gang
HG
Hu
Qiao-ling
QL
Bourhy
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D001921:Brain / Q000821:virology*; D004285:Dogs; D005455:Fluorescent Antibody Technique / Q000379:methods; D005838:Genotype; D019251:Nucleocapsid / Q000276:immunology*; D011820:Rabies virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral