Local administration of TGF-beta1 to reinforce the anterior abdominal wall in a rat model of incisional hernia.
The purpose of this study was to investigate different forms of the local application of TGF-beta(1) for augmentation of the anterior abdominal wall in an appropriate model of an incisional hernia. Sixty male Sprague-Dawley rats were divided into six groups. Artificial defects of the anterior abdominal wall were closed with one of the following methods: running Prolene suture, Vicryl mesh, prolene suture followed by an intramuscular injection of 1 mug TGF-beta(1), Vicryl mesh coated with 1 mug TGF-beta(1), and prolene suture coated with 1 mug TGF-beta(1). A control group did not receive any defect and treatment. Six weeks after operation, tensile strength, collagen content, gene expression of collagen I and III, blood vessels, and thickness of collagen fibres were evaluated. Tensile strength was strongest in the controls (14.2 (10.5-18 N)). There was no increase in tensile strength due to the administration of TGF-beta(1). On the contrary, bolus injection of the growth factor resulted in a significantly decreased strength of the wound tissue when compared to the groups 1, 4, 5, and 6 (9.1 (4.2-9.1 N)). These results correlated with the gene expression of collagen I and III. Local application of TGF-beta(1) did not augment the strength of the abdominal wall after 6 weeks.
Korenkov
M
M
Surgical Clinic, Department of Surgery, University of Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany. korenkov@ach.klinik.uni-mainz.de
Yuecel
N
N
Koebke
J
J
Schierholz
J
J
Morsczeck
Ch
Ch
Tasci
I
I
Neugebauer
E A M
EA
Nagelschmidt
M
M
D034861:Abdominal Wall / Q000502:physiology / Q000601:surgery*; D000818:Animals; D003094:Collagen / Q000032:analysis; D006555:Hernia, Ventral / Q000601:surgery*; D007273:Injections, Intramuscular; D008297:Male; D011098:Polyglactin 910; D011126:Polypropylenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage; D013526:Surgical Mesh; D013537:Sutures; D013718:Tensile Strength; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*; D053773:Transforming Growth Factor beta1; D014945:Wound Healing / Q000187:drug effects
D011126:Polypropylenes; D011994:Recombinant Proteins; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D011098:Polyglactin 910; D003094:Collagen