Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions.
The study describes the suitability of a colorimetric method (malachite green procedure) for detection of minimal amounts of phosphate (7.3-29.1 micromol/L) in different acidic solutions (hydrochloric acid, oxalic acid, maleic acid, perchloric acid, tartaric acid, citric acid, lactic acid and acetic acid) adjusted to pH 2.0. A mixture of the respective phosphate concentrations with distilled water served as control. The experiments were run with ten repeats in series. Assessment of intra- and interassay coefficient of variation and lower limit of quantification revealed that depending on the acid used, the applied method is a reliable and suitable tool to detect and quantify minimal phosphate contents in small samples of acidic solutions that have the potential to cause erosive dental lesions.
Attin
T
T
Department of Operative and Preventive Dentistry and Periodontology, Georg-August-University Göttingen, Robert-Koch-Strasse 40, 37075 Göttingen, Germany. thomas.attin@med.uni-goettingen.de
Becker
K
K
Hannig
C
C
Buchalla
W
W
Wiegand
A
A
D000143:Acids / Q000737:chemistry*; D003124:Colorimetry; D004396:Coloring Agents; D005240:Feasibility Studies; D007202:Indicators and Reagents; D010710:Phosphates / Q000032:analysis*; D012394:Rosaniline Dyes; D012996:Solutions / Q000737:chemistry; D017186:Titrimetry
D000143:Acids; D004396:Coloring Agents; D007202:Indicators and Reagents; D010710:Phosphates; D012394:Rosaniline Dyes; D012996:Solutions; C005095:malachite green