Discovery of selective aldo-keto reductase ligands--an on-bead assay strategy.
An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
Dixon
Seth
S
Department of Chemistry, University of California, Davis, 95616-5295, USA.
Robins
Lori
L
Elling
Robert A
RA
Liu
Ruiwu
R
Lam
Kit S
KS
Wilson
David K
DK
Kurth
Mark J
MJ
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D000449:Aldehyde Reductase / Q000378:metabolism; D000074408:Aldo-Keto Reductases; D008024:Ligands; D008856:Microscopy, Fluorescence; D008958:Models, Molecular
D008024:Ligands; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase