title: [Culture and identification of human adult Leydig cells].
abstract: OBJECTIVE
  To obtain a simple and effective method to isolate and purify adult Leydig cells.
  
  
  METHODS
  The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.
  
  
  RESULTS
  High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.
  
  
  CONCLUSIONS
  This method is easy and efficient for the isolation and purification of adult Leydig cells.
authors.affiliation: Department of Urology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Xiang-Zhou
authors.initials: XZ
authors.lastname: Sun
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Deng
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Guo
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Liu
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D006801:Humans; D007985:Leydig Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013739:Testosterone / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone