Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo.
The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.
Shakhar
Guy
G
Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA.
Lindquist
Randall L
RL
Skokos
Dimitris
D
Dudziak
Diana
D
Huang
Julie H
JH
Nussenzweig
Michel C
MC
Dustin
Michael L
ML
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology; D008164:Luminescent Proteins / Q000737:chemistry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D015778:Minor Histocompatibility Antigens; D010047:Ovalbumin / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D013047:Specific Pathogen-Free Organisms
D015703:Antigens, CD; D001426:Bacterial Proteins; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D008164:Luminescent Proteins; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; C054562:yellow fluorescent protein, Bacteria; D010047:Ovalbumin