Study on the characteristics of pectin-ketoprofen for colon targeting in rats.
Pectin-ketoprofen (PT-KP) prodrug with the potential for colon targeted delivery has been evaluated. A sensitive HPLC method was established for the determination of concentration of ketoprofen (KP) in rats. This method was also used to evaluate the colon targeting property of PT-KP. KP or PT-KP was given to rats by oral administration at a dosage of 10mg/kg. Plasma and the different parts of gastrointestinal (GI) tract were taken after 2, 4, 6, 8, 10 and 12h of oral administration of KP or PT-KP to rats and the concentration of KP was measured by HPLC. Preliminary experiments show KP distributes mainly in stomach, proximal small intestine and distal small intestine. However, KP released from PT-KP mainly distributes in cecum and colon. Therefore, this approach suggests that PT-KP prodrug has a good colon targeting property.
Xi
Miao Miao
MM
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Zhang
San Qi
SQ
Wang
Xin Yi
XY
Fang
Kun Quan
KQ
Gu
Yi
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism*; D005079:Excipients; D005260:Female; D007660:Ketoprofen / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D010368:Pectins / Q000008:administration & dosage*; D011355:Prodrugs / Q000493:pharmacokinetics*; D051381:Rats
D005079:Excipients; D011355:Prodrugs; D010368:Pectins; D007660:Ketoprofen