title: Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms.
abstract: Polyclonal antibodies obtained by immunization of rabbits with native form of weak toxin (WTX) from cobra Naja kaouthia venom efficiently interacted with WTX and a weak toxin from Naja oxiana venom, but not so with their denaturated forms. These antibodies could also bind with lower affinity other groups of three-fingered toxins: long-chain alpha-neurotoxins, muscarinic toxins and cytotoxins, but practically did not bind short-chain alpha-neurotoxins. The efficiency of toxin-antibody interaction depends on the group (weak toxins, long or short alpha-neurotoxins, cytotoxins etc.) to which the toxin belongs, but not on species of snake from which the toxin originates. There is a correlation between the results obtained and phylogenetic analysis of the three-fingered toxins which revealed that WTX is very close to other weak toxins, relatively close to long alpha-neurotoxins, cytotoxins and muscarinic toxins, but is distant from the short alpha-neurotoxins.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, V-437, Miklukho-Maklaya Street, 16/10, 117997 GSP, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kryukova
authors.forename: D Yu
authors.initials: DY
authors.lastname: Mordvintsev
authors.forename: S
authors.initials: S
authors.lastname: Daya
authors.forename: Yu N
authors.initials: YN
authors.lastname: Utkin
authors.forename: V I
authors.initials: VI
authors.lastname: Tsetlin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D004546:Elapid Venoms / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D017815:Elapidae / Q000502:physiology*; D000939:Epitopes; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D011489:Protein Denaturation; D011817:Rabbits
chemical_list: D004546:Elapid Venoms; D000939:Epitopes; C428174:Weak toxin, Naja