SECISDesign: a server to design SECIS-elements within the coding sequence.
CONCLUSIONS
SECISDesign is a server for the design of SECIS-elements and arbitrary RNA-elements within the coding sequence of an mRNA. The element has to satisfy both structure and sequence constraints. At the same time, a certain amino acid similarity to the original protein has to be kept. The designed sequence can be used for recombinant expression of selenoproteins in Escherichia coli.


BACKGROUND
The server is available at http://www.bio.inf.uni-jena.de/Software/SECISDesign/index.html.
Busch
Anke
A
Friedrich-Schiller-University Jena, Institute of Computer Science, Ernst-Abbe-Platz 2, 07743 Jena, Germany.
Will
Sebastian
S
Backofen
Rolf
R
D017076:Computer-Aided Design; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D020407:Internet; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D051140:Selenoproteins; D020539:Sequence Analysis, Protein / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software
D004251:DNA Transposable Elements; D011506:Proteins; D011994:Recombinant Proteins; D051140:Selenoproteins