title: Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma.
abstract: OBJECTIVE
  To study the effect of IGF-1/IGF-1R and gastrin/CCK-BR on carcinogenesis and development of human gastric carcinoma and to explore its mechanism and provide a credible theoretical foundation for early diagnosis and molecular therapy of gastric carcinoma.
  
  
  METHODS
  mRNA expression levels of IGF-1/IGF-1R and gastrin/CCK-BR were assessed by RT-PCR method in gastric cancer tissues, adjacent mucosa, and tumor-free tissues from 56 patients with gastric carcinoma and normal gastric mucosae from 56 healthy controls. Tissue specimens were obtained by biopsy and confirmed by histological evaluation.
  
  
  RESULTS
  The mRNA levels of IGF-1/IGF-1R were increased in gastric cancer tissues compared with normal tissues from healthy controls and successively increased in tumor-free tissues, adjacent mucosa, and gastric cancer tissues. The mRNA levels of gastrin/CCK-BR were increased in gastric cancer tissues compared with normal tissues from healthy controls. There was a significant difference between gastric cancer tissues and adjacent mucosa and tumor-free tissues, but the mRNA levels of gastrin were not significantly increased in adjacent mucosa and gastric cancer tissues compared with tumor-free tissues. The mRNA levels of CCK-BR were increased in gastric cancer tissues and adjacent mucosa compared with tumor-free tissues, but not significantly increased in adjacent mucosa and gastric cancer tissues compared with gastric cancer tissues.
  
  
  CONCLUSIONS
  Overexpression of IGF-1/IGF-1R and gastrin/CCK-BR promotes the disorderly proliferation of gastric mucosa epithelia and it is of great significance in the carcinogenesis and development of gastric carcinoma.
authors.affiliation: Department of Radiology, Affiliated Hospital of Binzhou Medical College, Binzhou 256-603, Shandong Province, China.
authors.forename: Ming-Dong
authors.initials: MD
authors.lastname: Zhao
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Dian-Jing
authors.initials: DJ
authors.lastname: Sun
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yu-Hao
authors.initials: YH
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
mesh_terms: D005755:Gastrins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D043887:Receptor, Cholecystokinin B / Q000235:genetics*; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D005755:Gastrins; D043887:Receptor, Cholecystokinin B; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1