title: [Influence of dermal template on the biomechanical compliance of wound tissue].
abstract: OBJECTIVE
  To investigate the influence of dermal template on the biomechanical compliance of wound tissue during wound repair.
  
  
  METHODS
  One hundred and forty-four SD rats subjected to full-thickness skin loss on the dorsum were enrolled in the study, and they were randomly divided into A (n = 6, without grafting on wound), B (n = 6, with full thickness skin grafting on wound), C (n = 6, with razor thin skin grafting on wound) and D [n = 6, with acellular dermal matrix (ADM) and razor thin skin grafting on wound] groups. The tissue samples from the wounds were harvested 1, 2, 4, 6, 12 and 20 weeks after the operation. The biomechanical compliance of the wound was assessed by Instron biomechanics tensiometer. The expression of alpha-SMA in the dermal fibroblasts of each group was determined by immunohistochemistry (ABC) method.
  
  
  RESULTS
  The biomechanical compliance of the wound in D group was higher than that in A and C groups (P < 0.05), but lower than that in B group during 4 to 20 weeks after operation (P < 0.05). The expression of alpha-SMA in D group (7.53 +/- 0.98)% was lower than that in A (26.99 +/- 2.90)% and C (2.18 +/- 2.79)% groups (P < 0.01), but higher than that in B group at 4 weeks after operation (P < 0.05).
  
  
  CONCLUSIONS
  Dermal template might affect the scar formation during wound healing, in improving wound healing quality by enhancing the biomechanical compliance of wound tissue.
authors.affiliation: Department of Burns, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P.R. China.
authors.forename: Ying-kai
authors.initials: YK
authors.lastname: Liu
authors.forename: Shu-liang
authors.initials: SL
authors.lastname: Lu
authors.forename: Chun
authors.initials: C
authors.lastname: Qing
authors.forename: Zhi-long
authors.initials: ZL
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Lin
authors.forename: Qing-shen
authors.initials: QS
authors.lastname: Hu
authors.forename: Zhen-jiang
authors.initials: ZJ
authors.lastname: Liao
authors.forename: Ji-xiang
authors.initials: JX
authors.lastname: Shi
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D003187:Compliance; D020405:Dermis / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016038:Skin Transplantation; D017768:Skin, Artificial; D017695:Soft Tissue Injuries / Q000503:physiopathology* / Q000601:surgery*; D014945:Wound Healing
chemical_list: D000199:Actins