Summary results from a pilot study conducted around an oil production platform on the Northwest Shelf of Australia.
The Australian Institute of Marine Science (AIMS) conducted a pilot study around the Harriet A oil production platform on the Northwest Shelf of Australia. We evaluated hepatic ethoxyresorufin-O-deethylase (EROD) activity, fluorescent aromatic compounds (FACs) in bile and immunodetection of CYP1A-like proteins in two Australian tropical fish species, Gold-Spotted Trevally (Carangoides fulvoguttatus) and Bar-Cheeked Coral Trout (Plectropomus maculatus) to assess exposure to petroleum hydrocarbons associated with produced formation water (PFW). Additionally, the incidence of hydrocarbon-degrading bacteria isolated from the liver and bile of all fish captured was examined. Low EROD activity was found in both species, with EROD activity in C. fulvoguttatus showing significant site differences. FACs and CYP1A protein levels in C. fulvoguttatus showed a clear trend in hydrocarbon exposure consistent with hydrocarbon chemistry data: Harriet A>Harriet C>reference site. P. maculatus showed elevated levels of FACs at Harriet A as compared to the reference site and demonstrated detectable levels of CYP1A-like proteins at these two sites. Hydrocarbon-degrading bacteria were found in the liver and bile of both species, yet there was no correlation by sites. Our results demonstrate that C. fulvoguttatus and P. maculatus have potential as indicator species for assessing the effects from exposure to petroleum hydrocarbons. Both FACs and CYP1A are providing warning signs that there is potential for biological effects on fish populations exposed to PFW around the Harriet A production platform.
King
Susan Codi
SC
Australian Institute of Marine Science, PMB #3, Townsville, Qld 4810, Australia. s.codiking@aims.gov.au
Johnson
Johanna E
JE
Haasch
Mary L
ML
Ryan
Daniel A J
DA
Ahokas
Jorma T
JT
Burns
Kathryn A
KA
D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001646:Bile / Q000382:microbiology; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004784:Environmental Monitoring / Q000706:statistics & numerical data*; D019652:Extraction and Processing Industry; D005453:Fluorescence; D019156:Indian Ocean; D008099:Liver / Q000382:microbiology; D010473:Perciformes / Q000378:metabolism* / Q000382:microbiology; D010578:Petroleum; D010865:Pilot Projects; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014904:Western Australia
D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1