A 13C CP/MAS and 31P NMR study of the interactions of dipalmitoylphosphatidylcholine with respirable silica and kaolin.
The interaction of silica and kaolin with dipalmitoylphosphatidylcholine (DPPC) has been studied using 13C and 31P solid state nuclear magnetic resonance spectroscopy. These studies explore the molecular interactions of these respirable dusts with a model lung surfactant species to characterize silica toxicity in mixed systems. The choline head group of DPPC was found to remain mobile when adsorbed on kaolin, in contrast to an immobile head group on silica. Further, glycerol carbon intensities were greatly diminished relative to that of choline carbons, a result attributed to broadening effects. These preliminary findings suggest that silica toxicity may not be related to choline mobility as previously noted.
Murray
David K
DK
National Institute for Occupational Health and Safety, 1095 Willowdale Road, M/S 3030, Morgantown, WV 26505, USA. dmurray@cdc.gov
Harrison
Joel C
JC
Wallace
William E
WE
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002250:Carbon Radioisotopes / Q000737:chemistry; D002794:Choline / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D007616:Kaolin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008567:Membranes, Artificial; D010761:Phosphorus Radioisotopes / Q000737:chemistry; D011663:Pulmonary Surfactants / Q000737:chemistry*; D012119:Respiration; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
D002250:Carbon Radioisotopes; D008567:Membranes, Artificial; D010761:Phosphorus Radioisotopes; D011663:Pulmonary Surfactants; D007616:Kaolin; D015060:1,2-Dipalmitoylphosphatidylcholine; D002244:Carbon; D012822:Silicon Dioxide; D002794:Choline; D005990:Glycerol