title: Serological markers during dengue 3 primary and secondary infections.
abstract: BACKGROUND
  The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied.
  
  
  OBJECTIVE
  To study the IgM, IgA and IgE specific antibody response in dengue 3 infected patients with different clinical picture and type of infection.
  
  
  METHODS
  One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies.
  
  
  CONCLUSIONS
  In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied.
authors.affiliation: Virology Department, PAHO/WHO Collaborating Center for Viral Diseases, Pedro Kourí Institute, Autopista Novia del Mediodía, Km 61/2, La Lisa, Ciudad de la Habana, P.O. Box: 601 Marianao 13, Havana City, Cuba (IPK). svazquez@ipk.sld.cu
authors.forename: S
authors.initials: S
authors.lastname: Vázquez
authors.forename: A B
authors.initials: AB
authors.lastname: Pérez
authors.forename: D
authors.initials: D
authors.lastname: Ruiz
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Pupo
authors.forename: N
authors.initials: N
authors.lastname: Calzada
authors.forename: L
authors.initials: L
authors.lastname: González
authors.forename: D
authors.initials: D
authors.lastname: González
authors.forename: O
authors.initials: O
authors.lastname: Castro
authors.forename: T
authors.initials: T
authors.lastname: Serrano
authors.forename: M G
authors.initials: MG
authors.lastname: Guzmán
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D015415:Biomarkers; D003462:Cuba; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D019595:Severe Dengue / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D015415:Biomarkers; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007073:Immunoglobulin E