A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes.
Epstein-Barr virus (EBV) infects human target cells mainly through gp350/220-CD21 and gp42-MHCII interactions; however, it has been shown that these interactions are dispensable for EBV-invasion of susceptible cells, suggesting that other viral proteins are involved in this process. It is probable that tegument BNRF1/p140 protein is involved in EBV-invasion of target cells, since anti-p140 antibodies inhibit EBV-infection of B-lymphocytes and there is evidence that part of the protein is located on virus surface. Sixty-six peptides, covering the entire BNRF1/p140 sequence, were synthesised and tested in lymphoblastoid cell line binding assays. Peptides 11465 and 11521 bound with high affinity to Raji, Ramos and P3HR-1 cells but not to erythrocytes, showing cell-binding behaviour similar to EBV. These two peptides induced antibodies recognising live EBV-infected cells. Interestingly, peptide-11521 (YVLQNAHQIACHFHSNGTDA) or antibodies induced by this peptide inhibited EBV-binding to B-lymphocytes, suggesting that this p140-region could be involved in EBV and B-lymphocyte interaction.
López
Ramsés
R
Fundación Instituto de Inmunología de Colombia (FIDIC), Universidad Nacional de Colombia, Avenida Carrera 50 No. 26-00, Bogotá, Colombia.
Urquiza
Mauricio
M
Patino
Helena
H
Suárez
Jorge
J
Reyes
Claudia
C
Patarroyo
Manuel A
MA
Patarroyo
Manuel E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000821:virology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology*; D010455:Peptides; D016133:Polymerase Chain Reaction; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000494:pharmacology*
D000914:Antibodies, Viral; C506671:BNRF1 protein, Epstein-Barr virus (1221-1240); C062724:P140 protein, Epstein-barr virus; D010446:Peptide Fragments; D010455:Peptides; D014759:Viral Envelope Proteins