title: [Establishment of COL1A1-EGFP stably transfected ROS17/2.8 cell lines].
abstract: OBJECTIVE
  To obtain ROS17/2.8 cell lines which were stably expressing EGFP reporter gene drived by COL1A1 promoter.
  
  
  METHODS
  A 3.6 Kb COL1A1 promoter from rat was cloned into pMD-18-T vector by PCR. This amplified promoter vector was digested to get several different length fragments which were then fused with EGFP reporter gene to construct eukaryotic expression vectors. ROS17/2.8 cell was stably transfected with these vectors by LipofectAMINE(TM) and selected by G418.
  
  
  RESULTS
  The COL1A1-EGFP stably transfected cell lines were established.
  
  
  CONCLUSIONS
  The cell lines will be useful for studying the effects of microgravity on the activity of COL1A1 promoter and expression of gene related with bone form.
authors.affiliation: Institute of Space Medico-Engineering, Beijing, China.
authors.forename: Zhong-quan
authors.initials: ZQ
authors.lastname: Dai
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Li
authors.forename: Bai
authors.initials: B
authors.lastname: Ding
authors.forename: Yu-guo
authors.initials: YG
authors.lastname: Zhang
authors.forename: Peng-peng
authors.initials: PP
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D010006:Osteoblasts; D012516:Osteosarcoma / Q000235:genetics; D016133:Polymerase Chain Reaction; D051381:Rats; D018474:Weightlessness Simulation
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase