[Analysis of angiogenesis associated factors of retinoblastoma cell line and tumor tissues].
OBJECTIVE
To investigate the factors regulating angiogenesis of retinoblastoma.


METHODS
Cultured retinoblastoma cells from HXO-Rb-44 cell lines were inoculated subcutaneously into the leg of nude-mice. Experimental retinoblastoma was studied by immunohistochemistry staining for the expression of angiogenesis factors (VEGF-A, VEGF-C, VEGF-D and bFGF), vascular endothelial cell membrane receptor tyrosine kinases (Flt-1, Flt-4, Flk-1, Tie-2 and FGFR), transcription regulated factor, Hif-1, matrix metalloproteinase (MMP-2, MMP-7 and MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1 and TIMP-2).


RESULTS
There were plenty of capillaries and small vessels in the retinoblastoma. Some retinoblastoma cells expressed Hif-1 and most cells expressed VEGF at a high level, especially VEGF-D. Flt-1, Flt-4 and FGFR were found in vascular endothelial cells in the retinoblastoma. Three matrix metalloproteinases were positive and two tissue inhibitors of metalloproteinases were negative in the retinoblastoma.


CONCLUSIONS
Down-regulated TIMP and up-regulated MMP and VEGF signal transduction play an important role in the occurrence of angiogenesis in the retinoblastoma. This could be considered as the target of retinoblastoma treatment.
Wu
Ji-hong
JH
Central Experimental Laboratory, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Xu
Ping
P
Yi
Miao-ying
MY
Wang
Feng
F
Wang
Xiao-li
XL
Huang
Qian
Q
D000818:Animals; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D020782:Matrix Metalloproteinases / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D019572:Retinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D012175:Retinoblastoma / Q000378:metabolism* / Q000473:pathology; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis*
D019714:Tissue Inhibitor of Metalloproteinases; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D020782:Matrix Metalloproteinases