title: CC chemokine receptor 4 ligand production by bronchoalveolar lavage fluid cells in cigarette-smoke-associated acute eosinophilic pneumonia.
abstract: We examined the production of macrophage-derived chemokine (MDC/CCL22) and thymus- and activation-regulated chemokine (TARC/CCL17) by bronchoalveolar lavage fluid (BALF) cells in cigarette-smoke-associated acute eosinophilic pneumonia (CS-AEP). The CC Chemokine Receptor 4 (CCR4) ligand levels in BALF from patients with CS-AEP were considerably higher than those in healthy volunteers and correlated well with Th2 cytokine levels. Interleukin-4 enhanced CCR4 ligand production. MDC expression was observed in CD68-positive cells from patients with CS-AEP and in healthy control smokers. In contrast, TARC expression in CD68- or CD1a-positive cells was detected only in CS-AEP. An in vivo cigarette smoke challenge test induced increases in CCR4 ligands in the BALF and in the cultured supernatant of BALF adherent cells. These results suggest that alveolar macrophages and dendritic cells contribute to the pathogenesis of CS-AEP by generating CCR4 ligands, probably in response to cigarette smoke.
authors.affiliation: Division of Pulmonary Disease, Third Department of Internal Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan. nureki@med.oita-u.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nureki
authors.forename: Eishi
authors.initials: E
authors.lastname: Miyazaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Ando
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Tsuda
mesh_terms: D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000378:metabolism; D008024:Ligands; D011657:Pulmonary Eosinophilia / Q000209:etiology / Q000378:metabolism*; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: C106230:CCL17 protein, human; C515482:CCL22 protein, human; C515354:CCR4 protein, human; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC; D008024:Ligands; D054398:Receptors, CCR4; D019707:Receptors, Chemokine; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor