Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein.
To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH.
Matsuno
Akira
A
Department of Neurosurgery, Teikyo University Ichihara Hospital, 3426-3 Anesaki, Ichihara City, Chiba 299-0111, Japan. akirakun@med.teikyo-u.ac.jp
Mizutani
Akiko
A
Itoh
Johbu
J
Takekoshi
Susumu
S
Nagashima
Tadashi
T
Okinaga
Hiroko
H
Takano
Koji
K
Osamura
R Yoshiyuki
RY
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002460:Cell Line / Q000378:metabolism*; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics*; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; D013006:Growth Hormone; D002118:Calcium