title: Nucleolin is regulated both at the level of transcription and translation.
abstract: Nucleolin is a multi-functional protein that is located to the nucleolus. In tissue culture cells, the stability of nucleolin is related to the proliferation status of the cell. During development, rat cardiomyocytes proliferate actively with increases in the mass of the heart being due to both hyperplasia and hypertrophy. The timing of this shift in the phenotype of the myocyte from one capable of undergoing hyperplasia to one that can grow only by hypertrophy occurs within 4 days of post-natal development. Thus, cardiomyocytes are an ideal model system in which to study the regulation of nucleolin during growth in vivo. Using Western blot and quantitative RT-PCR (TaqMan) we found that the amount of nucleolin is regulated both at the level of transcription and translation during the development of the cardiomyocyte. However, in cells which had exited the cell cycle and were subsequently given a hypertrophic stimulus, nucleolin was regulated post-transcriptionally.
authors.affiliation: Cardiovascular Research Group, School of Pharmacy, The University of Reading, UK.
authors.forename: Katrina
authors.initials: K
authors.lastname: Bicknell
authors.forename: Gavin
authors.initials: G
authors.lastname: Brooks
authors.forename: Pete
authors.initials: P
authors.lastname: Kaiser
authors.forename: Hongying
authors.initials: H
authors.lastname: Chen
authors.forename: Brian K
authors.initials: BK
authors.lastname: Dove
authors.forename: Julian A
authors.initials: JA
authors.lastname: Hiscox
mesh_terms: D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002454:Cell Differentiation; D049108:Cell Enlargement; D004247:DNA / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D066298:In Vitro Techniques; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection; D000097796:Nucleolin
chemical_list: D004247:DNA; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D000097796:Nucleolin