Expression of cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor.
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
Huang
Yinping
Y
Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Ye
Duyun
D
Wu
Ping
P
Huang
Yanjun
Y
Zhang
Li
L
Zhou
Xiaoyan
X
Huang
Yunfeng
Y
Yuan
Ping
P
Zhang
Daijuan
D
Wan
Jingyuan
J
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007745:Labor Onset / Q000378:metabolism*; D008969:Molecular Sequence Data; D009215:Myometrium / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017421:Sequence Analysis
D012333:RNA, Messenger; D051546:Cyclooxygenase 2