title: One- and two-dimensional electrophoretic identification of IgE-binding polypeptides of Lupinus albus and other legume seeds.
abstract: The prevalence of food allergies in the world population requires integrated approaches to identify new potential allergens, especially those of plant origin. The aim of this work was the allergen in vitro analysis of Lupinus albus seed proteome, a promising food protein source, and the assessment of IgE cross-reactivities with other more diffused legume species. A combination of one- and two-dimensional gel electrophoresis and immunoblotting analyses with specific IgGs for band identification and lupin-sensitized patients' circulating IgEs for allergenicity studies has been used. Two lupin proteins, namely, conglutin gamma and 11S globulin basic subunits, strongly reacted with all patients' sera. Also, cross-reactivities with the homologous polypeptides of other legume species were observed. Otherwise, no reaction at all was detected with a 2S-type lupin protein. This global electrophoretic approach has allowed the identification of a new potential lupin allergen and confirmed the cross-reactivity among the legume 11S globulin basic subunits.
authors.affiliation: Department of AgriFood Molecular Sciences, State University of Milan, Via Celoria 2, I-20133 Milan, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Magni
authors.forename: Anita
authors.initials: A
authors.lastname: Herndl
authors.forename: Elena
authors.initials: E
authors.lastname: Sironi
authors.forename: Alessio
authors.initials: A
authors.lastname: Scarafoni
authors.forename: Cinzia
authors.initials: C
authors.lastname: Ballabio
authors.forename: Patrizia
authors.initials: P
authors.lastname: Restani
authors.forename: Roberto
authors.initials: R
authors.lastname: Bernardini
authors.forename: Elio
authors.initials: E
authors.lastname: Novembre
authors.forename: Alberto
authors.initials: A
authors.lastname: Vierucci
authors.forename: Marcello
authors.initials: M
authors.lastname: Duranti
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007073:Immunoglobulin E / Q000378:metabolism*; D007525:Isoelectric Focusing; D031297:Lupinus / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000276:immunology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D010455:Peptides; D007073:Immunoglobulin E