[Two-dimensional electrophoresis and mass spectrometry analysis on effects of trichloroethylene on protein of L-02 liver cells].
OBJECTIVE
To study the effects of trichloroethylene (TCE) on the protein in L-02 cells in vitro.


METHODS
Thiazolyl blue and Trypan blue tests were used to investigate the cytotoxicity of TCE to L-02 liver cell. The 2-D electrophoresis was used to analyse the expression of proteins in L-02 liver cells. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).


RESULTS
When the concentration of TCE exceeded 30 micromol/L, there was distinct cytotoxicity to L-02 cell (P < 0.05). Selected 40 micromol/L to treat L-02 liver cells and analyze the differential proteome expression, the results showed that the expression level of 37 protein spots was up-regulated and 15 protein spots was down-regulated. And 15 proteins were identified by MALDI-TOF-TOF-MS.


CONCLUSIONS
TCE can change the proteome expression of L-02 liver cell. It should provide the fundamental information to identify proteins related to TCE in further study.
Huang
Hai-yan
HY
Laboratory of food and Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen 510080, China.
Liu
Jian-jun
JJ
Zhuang
Zhi-xiong
ZX
Li
Xi-yi
XY
Xu
Xin-yun
XY
Wei
Qin-zhi
QZ
Yang
Xiao-hua
XH
D018685:Anesthetics, Inhalation / Q000494:pharmacology; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014241:Trichloroethylene / Q000494:pharmacology*
D018685:Anesthetics, Inhalation; D011506:Proteins; D020543:Proteome; D014241:Trichloroethylene