title: A new simply and effective fractionation method for cylindrospermopsin analyses.
abstract: A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50% methanol containing 1% formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Aramaki-aza aoba 6-6-20, Aoba-ku, Sendai 980-8579, Japan. kubo@mail.kankyo.tohoku.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Sano
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kunimitsu
authors.initials: K
authors.lastname: Kaya
mesh_terms: D000470:Alkaloids; D001427:Bacterial Toxins; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid; D000087522:Cyanobacteria Toxins; D046891:Cylindrospermopsis / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil