title: [Association between familial febrile convulsions and HCN2 gene].
abstract: OBJECTIVE
  To investigate the possible association of familial febrile convulsions with HCN2 gene.
  
  
  METHODS
  PCR was conducted on the DNA of peripheral blood white cells from 60 children with familial febrile convulsion (FC) of Han nationality population in northern China aged 1.5 +/- 1.0 (8 months to 5 years old), to amplify the exons of HCN2 gene. The PCR products underwent sequencing to identify the possible mutations. 101 normal children from the same area were used as controls.
  
  
  RESULTS
  No mutation was found in the exons of HCN2 gene, however, 14 single nucleotide polymorphisms (SNPs) were found among which there were 8 newly identified SNPs. Using 9 SNPs as markers, association study was conducted between the FC group and control group. There were no significant differences in allele frequencies and genotype frequencies of the 9 SNPs between the FC group and control group.
  
  
  CONCLUSIONS
  HCN2 may not be a susceptibility gene for FC in Chinese population.
authors.affiliation: Department of Pediatrics, First Hospital of Peking University, Beijing 100034, China.
authors.forename: Yi-Nang
authors.initials: YN
authors.lastname: Ma
authors.forename: Zhi-Yue
authors.initials: ZY
authors.lastname: Chen
authors.forename: Li-Pin
authors.initials: LP
authors.lastname: Zou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Niu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Xu
authors.forename: Pei
authors.initials: P
authors.lastname: Pei
authors.forename: Ding-Fang
authors.initials: DF
authors.lastname: Bu
authors.forename: Yu
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000483:Alleles; D002675:Child, Preschool; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007223:Infant; D007473:Ion Channels / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D015221:Potassium Channels; D003294:Seizures, Febrile / Q000235:genetics*
chemical_list: C578710:HCN2 protein, human; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D015221:Potassium Channels