title: Normal growth rate in rats is recovered after a period of zinc deficiency by restoration of zinc supply by means of a zinc-fortified Petit Suisse cheese.
abstract: Fortification of a Petit Suisse cheese with zinc sulfate and zinc gluconate stabilized with glycine was used as a tool to overcome zinc-deficiency effects on total-body growth and skeletal growth. Animals were divided in 4 groups of 10 rats: basal (B), control (C), depletion-repletion 1 (DR1), and depletion-repletion 2 (DR2). These four groups were fed with four diets: basal (2 ppm Zn), control (30 ppm Zn), DR1, and DR2; they received a basal diet for 14 d and a control diet for the other 14 d of the experiment, using zinc sulfate for DR1 and zinc gluconate stabilized with glycine for DR2. After 28 d of the experiment, total-body weight and weight gain of the control and DR1 and DR2 animals were not statistically different (p<0.05), Femur weight and femur zinc content of DR1 and DR2 did not achieve the values of control animals (p<0.05), but they were higher than that of basal animals. Our results show that restoration of dietary zinc levels by means of food fortification normalized weight gain, as an indicator of total-body growth, and presented a trend to normalize bone weight, as a marker of skeletal growth, in young rats and independently of the zinc source used.
authors.affiliation: Radioisotope Laboratory and Stable Isotope Laboratory Applied to Biology and Medicine, Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires. Junín 956 1113-Ciudad de Buenos Aires, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Salgueiro
authors.forename: N
authors.initials: N
authors.lastname: Leonardi
authors.forename: M
authors.initials: M
authors.lastname: Zubillaga
authors.forename: R
authors.initials: R
authors.lastname: Weill
authors.forename: C
authors.initials: C
authors.lastname: Goldman
authors.forename: G
authors.initials: G
authors.lastname: Calmanovici
authors.forename: A
authors.initials: A
authors.lastname: Barrado
authors.forename: M Martinez
authors.initials: MM
authors.lastname: Sarrasague
authors.forename: J
authors.initials: J
authors.lastname: Boccio
mesh_terms: D000818:Animals; D002611:Cheese; D003677:Deficiency Diseases / Q000178:diet therapy*; D005269:Femur / Q000033:anatomy & histology / Q000737:chemistry; D005527:Food, Fortified; D005942:Gluconates / Q000627:therapeutic use; D005998:Glycine / Q000627:therapeutic use; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain / Q000187:drug effects; D015032:Zinc / Q000172:deficiency* / Q000627:therapeutic use; D019287:Zinc Sulfate / Q000627:therapeutic use*
chemical_list: D005942:Gluconates; D006454:Hemoglobins; C437626:zinc gluconate glycine; D019287:Zinc Sulfate; D015032:Zinc; D005998:Glycine