title: Effects of fatty acids on growth and poly-3-hydroxybutyrate production in bacteria.
abstract: The effects of saturated and unsaturated fatty acids (lauric acid, palmitic acid, steric acid, oleic acid, linoleic acid, soybean oil) on Sphaerotilus natans, 0B17 (Pseudomonas sp.), and recombinant Escherichia coli DH5(/pUC19/CAB were studied. Oleic acid enhances Poly-3-hydroxybutyrate (PHB) production in these three bacterial strains, suggesting that the single double bond of the acid activates the polyhydroxylkanoate accumulation enzymatic reaction. Under the effect of lauric acid and linoleic acid, the growth of S. natans and 0B17 were totally inhibited. However, the enhanced PHB accumulation in recombinant E. coli was observed.
authors.affiliation: State Key Lab of Chinese Med and Molecular Pharmacology, Shenzen, China.
authors.forename: K W
authors.initials: KW
authors.lastname: Lo
authors.forename: H
authors.initials: H
authors.lastname: Chua
authors.forename: H
authors.initials: H
authors.lastname: Lawford
authors.forename: W H
authors.initials: WH
authors.lastname: Lo
authors.forename: Peter H F
authors.initials: PH
authors.lastname: Yu
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D013045:Species Specificity; D042744:Sphaerotilus / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D000094122:Polyhydroxybutyrates
chemical_list: D005227:Fatty Acids; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates