title: Flavoridin inhibits Yersinia enterocolitica uptake into fibronectin-adherent HeLa cells.
abstract: In this study, three structurally distinct disintegrins (flavoridin, echistatin, kistrin) were used as molecular probes to further characterize the molecular mechanisms underlying Yersinia enterocolitica infection of host cells. The activity of the three disintegrins on Y. enterocolitica uptake into fibronectin-adherent HeLa cells was evaluated at disintegrin doses which were non-cytotoxic and unable to induce cell detachment. Flavoridin resulted to be the most effective in inhibiting bacterial entry into host cells; echistatin was almost 50% less effective than flavoridin, whereas kistrin was definitely inactive. Our results suggest that alpha(5)beta(1) integrin receptor, which binds flavoridin with higher affinity than the other two disintegrins, plays a major role in Y. enterocolitica uptake into HeLa cells. Furthermore, flavoridin binding to this integrin prevented the disruption of the functional complex FAK-Cas, which occurs in the Y. enterocolitica uptake process.
authors.affiliation: Dipartimento di Patologia e Sanità Animale, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Scibelli
authors.forename: Gianluca
authors.initials: G
authors.lastname: Matteoli
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
authors.forename: Elena
authors.initials: E
authors.lastname: Alimenti
authors.forename: Ludovico
authors.initials: L
authors.lastname: Dipineto
authors.forename: Luigi Michele
authors.initials: LM
authors.lastname: Pavone
authors.forename: Rossella
authors.initials: R
authors.lastname: Della Morte
authors.forename: Lucia Francesca
authors.initials: LF
authors.lastname: Menna
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fioretti
authors.forename: Norma
authors.initials: N
authors.lastname: Staiano
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology*; D019483:Disintegrins / Q000494:pharmacology*; D005353:Fibronectins; D051417:Focal Adhesion Kinase 1 / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects* / Q000382:microbiology; D006801:Humans; D039081:Integrin alpha5beta1 / Q000737:chemistry / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D015335:Molecular Probes; D010455:Peptides / Q000494:pharmacology*; D015008:Yersinia enterocolitica / Q000187:drug effects* / Q000472:pathogenicity
chemical_list: D003435:Crotalid Venoms; D019483:Disintegrins; D005353:Fibronectins; D039081:Integrin alpha5beta1; D036341:Intercellular Signaling Peptides and Proteins; D015335:Molecular Probes; D010455:Peptides; C069044:flavoridin protein, Trimeresurus; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human