Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications.
We present a method for performing polymerase chain reaction (PCR) using isolated droplets flowing in an immiscible fluorinated solvent system. Thanks to an optimized control of interfacial properties, we could achieve in this capillary-based system reproducible amplification factors, without any detectable contamination between neighboring droplets. The system is readily amenable to further miniaturization and automation and serves as the first step toward a clinically viable, high-throughput, quantitative continuous flow PCR apparatus.
Dorfman
Kevin D
KD
Laboratoire Physicochimie-Curie, UMR/CNRS 168, Institut Curie Section de Recherche, 26 Rue d'Ulm, F-75248 Paris Cedex, France.
Chabert
Max
M
Codarbox
Jean-Hugues
JH
Rousseau
Gilles
G
de Cremoux
Patricia
P
Viovy
Jean-Louis
JL
D004247:DNA / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D004247:DNA