title: Apoptosis induced by one new podophyllotoxin glucoside in human carcinoma cells.
abstract: 4-Demethyl-picropodophyllotoxin 7'-O-beta-D-glucopyranoside (4DPG), a new podophyllotoxin glucoside, was isolated from the rhizomes of Sinopodophyllum emodi (Wall.) Ying and showed cytotoxic effects in human carcinoma cells. Among the target cells (HeLa, A2 and SH-SY5Y), the cytotoxic effects of 4DPG showed dose- and time-dependency. Furthermore, the cervical carcinoma cell line, HeLa, was more sensitive to 4DPG. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at the G1 phase, and a concomitant increase of cell population at the G2/M phase. 4DPG also caused DNA fragmentation in HeLa cells. Treatment with 0.1 microM 4DPG increased p53 expression and Bax/Bcl-2 ratio in HeLa cells, as well as in A2 cells. These results suggested that 4DPG-induced apoptosis might be through a p53-dependent pathway.
authors.affiliation: Life Sciences College, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Qiu-Ying
authors.initials: QY
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Chang-Qi
authors.initials: CQ
authors.lastname: Zhao
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Ding
authors.forename: Yong-Gong
authors.initials: YG
authors.lastname: Zhai
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011035:Podophyllum; D027343:Rhizome
chemical_list: C440054:4-demethyl-picropodophyllotoxin 7'-O-glucopyranoside; D000972:Antineoplastic Agents, Phytogenic; D005960:Glucosides; D010936:Plant Extracts; D011034:Podophyllotoxin