title: Hydrolysis of polyesters by serine proteases.
abstract: The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene succinate) (PBS), poly(butylene succinate-co-adipate) (PBS/A), poly[oligo(tetramethylene succinate)-co-(tetramethylane carbonate)] (PBS/C), and poly(epsilon-caprolactone) (PCL). alpha-Chymotrypsin could degrade PLA and PEA with a lower activity on PBS/A. Proteinase K and subtilisin degraded almost all substrates other than PHB. Trypsin and elastase had similar substrate specificities to alpha-chymotrypsin.
authors.affiliation: Institute of Agricultural Science & Technology, Chonbuk National University, Jeonju, 561-756, Korea.
authors.forename: Hyun-A
authors.initials: HA
authors.lastname: Lim
authors.forename: Takao
authors.initials: T
authors.lastname: Raku
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tokiwa
mesh_terms: D000272:Adipates / Q000378:metabolism; D002072:Butylene Glycols / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D011095:Polyethylenes / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*; D013379:Substrate Specificity; D020860:Subtilisin / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D000272:Adipates; D002072:Butylene Glycols; D002918:Chymotrypsin; D019286:Endopeptidase K; D006885:Hydroxybutyrates; D010196:Pancreatic Elastase; D011091:Polyesters; D011095:Polyethylenes; D011108:Polymers; D012697:Serine Endopeptidases; D020860:Subtilisin; D013386:Succinates; D014357:Trypsin; C089797:bionole; C475864:poly(butylene succinate-co-butylene adipate); C443715:poly(ethylene succinate); C016240:polycaprolactone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C033616:poly(lactide); C117608:alpha-chymotrypsin