Renal expression of the essential genes associated with sodium transport following a total proctocolectomy in rats.
The water and electrolyte balance is regulated by the renin-angiotensin-aldosterone system in the kidney. We previously reported that the levels of circulating aldosterone dramatically increased following a total proctocolectomy in rats. However, there is no direct evidence regarding whether renal adaptation is accelerated by the induction of aldosterone-associated molecules. To explore this question, Sprague-Dawley rats underwent total proctocolectomies and then were killed 8 weeks later. We investigated the renal expression of 11beta-hydroxysteroid dehydrogenase type 2, the alpha-, beta-, and gamma-subunits of the epithelial sodium channel, and the alpha1- and beta1-subunits of Na+,K+-ATPase mRNAs because those molecules are responsible for the aldosterone specificity for mineralocorticoid receptor, amiloride-sensitive sodium absorption, and sodium extrusion from distal tubules, respectively. A Northern blot analysis demonstrated the kidney to exhibit mRNA induction for all of these molecules, thus supporting the idea that renal adaptation following a total proctocolectomy depends, at least in part, upon the molecular induction which is principally regulated by circulating aldosterone.
Fukushima
Kouhei
K
Department of Surgery, Tohoku University, Graduate School of Medicine, 1-1 Seiryomachi, Sendai, 980-8574, Japan.
Sato
Shun
S
Naito
Hiroo
H
Funayama
Yuji
Y
Shibata
Chikashi
C
Sasaki
Iwao
I
D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000222:Adaptation, Physiological; D000818:Animals; D015152:Blotting, Northern; D004847:Epithelial Cells / Q000201:enzymology; D053503:Epithelial Sodium Channels; D015870:Gene Expression; D007150:Immunohistochemistry; D007668:Kidney; D008297:Male; D011184:Postoperative Period; D016737:Proctocolectomy, Restorative; D012333:RNA, Messenger / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000502:physiology*; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D053503:Epithelial Sodium Channels; D012333:RNA, Messenger; D015222:Sodium Channels; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2