title: Peptide photodissociation at 157 nm in a linear ion trap mass spectrometer.
abstract: The photodissociation by 157 nm light of singly- and doubly-charged peptide ions containing C- or N-terminal arginine residues was studied in a linear ion trap mass spectrometer. Singly-charged peptides yielded primarily x- and a-type ions, depending on the location of the arginine residue, along with some related side-chain fragments. These results are consistent with our previous work using a tandem time-of-flight (TOF) instrument with a vacuum matrix-assisted laser desorption/ionization (MALDI) source. Thus, the different internal energies of precursor ions in the two experiments seem to have little effect on their photofragmentation. For doubly-charged peptides, the dominant fragments observed in both photodissociation and collisionally induced dissociation (CID) experiments are b- and y-type ions. Preliminary experiments demonstrating fragmentation of multiply-charged ubiquitin ions by 157 nm photodissociation are also presented.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Kim
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Thompson
authors.forename: James P
authors.initials: JP
authors.lastname: Reilly
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D002417:Cattle; D006736:Horses; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D010455:Peptides; D025801:Ubiquitin; D001120:Arginine