Effect of okadaic acid on integrins and structural proteins in BE(2)-M17 cells.
Okadaic acid (OA), an algal toxin, is known to induce Diarrhetic Shellfish Poisoning and apoptosis in a variety of cell lines. One of the main targets of OA is the actin cytoskeleton which can be modulated by integrins and other structural proteins. In this paper we studied the role of these proteins and skeletal structures on OA-induced apoptosis in neuroblastoma cells. Results show that beta1 integrin and vinculin are down-regulated when cells were exposed to OA. We observed an interaction between talin and beta1 integrin that is impaired in OA treated cells.
Santaclara
Francisco
F
Microbiology and Biotoxins Area, ANFACO-CECOPESCA, Campus Univ Vigo, 36310 Pontevedra, Spain. agcabado@anfaco.es
Lago
Jorge
J
Vieites
Juan M
JM
Cabado
Ana G
AG
D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D019319:Okadaic Acid / Q000494:pharmacology*; D016608:Talin / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
D003598:Cytoskeletal Proteins; D019012:Integrin beta1; D016023:Integrins; D016608:Talin; C488955:VCL protein, human; D016596:Vinculin; D019319:Okadaic Acid