[Culture of human epidermal stem cells in different media and their biological characteristics].
OBJECTIVE
To select the appropriate media to culture the epidermal stem cells in vitro, and to observe the biological characteristics of the epidermal stem cells.


METHODS
The epidermal stem cells were cultured in five different media, including FAD, FAD+1 ng/ml bFGF, FAD+5 ng/ml bFGF, FAD+10 ng/ml bFGF and K-SFM, and the same fetus fibroblasts were used as the nutrient cells. The proliferation ability was investigated by cell growth curve and MTT detection. Then the biological characteristics of epidermal stem cells were observed through phase-contrast microscope, cell growth curve, BrdU detection and FBM analysis.


RESULTS
The epidermal stem cells grew best in FAD with bFGF and nutrient cells. And the epidermal stem cells retained proliferative capacity, and formed larger and more expandable clones in vitro. And 80.2% of the cells show a G0/G1 cycle, and the cells had long cell proliferation cycle.


CONCLUSIONS
The above results demonstrate that the media with bFGF and the use of nutrient layer were appropriate to culture epidermal stem cell in vitro. And the epidermal stem cells have a slow cell cycle, characteristics of immaturity.
Dong
Rui
R
Department of Oral Histology and Pathology, College of Stomatology, Fourth Military Medical University, Xi'an Shaanxi, 710032, PR China.
Jin
Yan
Y
Lui
Yuan
Y
D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006801:Humans; D008858:Microscopy, Phase-Contrast; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D005182:Flavin-Adenine Dinucleotide