An aptamer-based protein biochip.
The establishment of an aptamer-based biochip for protein detection is described. Using a model system comprising human IgE as the analyte and single-stranded DNA aptamers specific for IgE or anti-IgE antibodies as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. Aptamer-based analyte detection on glass slides could clearly be demonstrated at minimum concentrations of 10 ng/mL IgE. In addition, we successfully showed specific analyte recognition in complex protein samples by the aptamer-based biochip system. Using DNA aptamers specific for human thrombin as an additional model receptor/ligand system, dual protein detection on a single slide could be proven. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific protein detection, representing an innovative tool for future proteomics.
Stadtherr
Karin
K
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg, Germany.
Wolf
Hans
H
Lindner
Petra
P
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D005898:Glass / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D015394:Molecular Structure; D008970:Molecular Weight; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013917:Thrombin / Q000032:analysis*
D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E; D013917:Thrombin