[The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus].
OBJECTIVE
To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients.


METHODS
Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA.


RESULTS
In 162 serum samples, 90.2% (55/61) became N protein positive in 1 - 5 days and 92.8% (13/14) became positive IgM and IgG in 15 - 18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3% (14/24) in 1 - 5 days and 71.4% (10/14) in 6 - 9 days after the onset.


CONCLUSIONS
Other than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.
Wang
Ming
M
Guangzhou Centre of Disease Control and Prevention, Guangzhou 510080, China.
Gao
Yang
Y
Zhou
Duan-hua
DH
Wu
Xin-wei
XW
Chen
Xiao-shuang
XS
Di
Biao
B
Liu
Yu-fei
YF
Chen
Fang
F
Du
Lin
L
Xu
Hui-fang
HF
Gu
Jing
J
Zheng
Bo-jian
BJ
Xu
Jian-guo
JG
D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D019590:Nucleocapsid Proteins / Q000097:blood; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic