Molecular studies of Onchocerca volvulus isolates from Mexico.
DNA from Onchocerca volvulus from Oaxaca and Chiapas, Mexico were used as templates to amplify members of the O-150 Onchocerca specific repeat sequence family. The resulting PCR amplicons all hybridized with OVS2, an oligonucleotide that has been previously shown to recognize amplicons derived from O. volvulus with 100% sensitivity. However, when PCR products amplified from the O. volvulus specific plasmid pOVS134 were used as a probe, most samples did not hybridize. Similarly, when PCR products amplified from DNA isolated from adult O. volvulus from Oaxaca were used as a probe, amplicons from adult worms from both Oaxaca and Chiapas were recognized, but PCR products from infected black flies from Chiapas were not recognized. Amplicons derived from an adult worm from Chiapas hybridized with PCR products produced from adult parasites from both Oaxaca and Chiapas and to PCR products derived from the DNA of infected black flies from Chiapas. These data, when taken together, suggest that differences exist among the repeat sequence populations of parasites from Oaxaca and Chiapas in Mexico, suggesting that the O-150 repeat sequence family may be a useful tool for biogeographic studies of O. volvulus in the Americas.
Ramírez-Ramírez
Alicia
A
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, Col. Casco de Santo Tomás, C.P. 11340, México D.F., Mexico.
Sánchez-Tejeda
Gustavo
G
Méndez-Galván
Jorge
J
Unnasch
Thomas R
TR
Monroy-Ostria
Amalia
A
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D018509:DNA, Helminth / Q000737:chemistry / Q000378:metabolism*; D005784:Gene Amplification; D017238:Genes, Helminth; D014644:Genetic Variation; D005843:Geography; D007303:Insect Vectors / Q000235:genetics / Q000469:parasitology; D007814:Larva / Q000235:genetics; D008800:Mexico; D009693:Nucleic Acid Hybridization; D017181:Onchocerca volvulus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012843:Simuliidae / Q000235:genetics / Q000469:parasitology; D013698:Templates, Genetic
D015342:DNA Probes; D018509:DNA, Helminth