title: Effect of withdrawal of fluoride-containing toothpaste on the interproximal plaque microflora.
abstract: To compare the effects of fluoride-containing and fluoride-free toothpaste on plaque microflora, 15 subjects were enrolled in a double-blind crossover trial. All subjects used a fluoride toothpaste for 7 days before the trial started. Then, 4 interproximal sites per subject were professionally cleaned and subjects used one of the toothpastes for 5 days. On the 5th day plaque was collected from 2 sites, 12 and 6 h after toothpaste use. There was no difference between the groups in the numbers or proportions of aciduric bacteria (recovered at pH 4.8 or 5.2), or of yeasts, neisseriae, lactobacilli or streptococci (total or individual species, including Streptococcus mutans). However, the numbers and proportions of Gram-positive pleomorphic rods, primarily Actinomyces naeslundii, increased in 6-hour samples from subjects using fluoride toothpaste. The data suggest that the anti-caries effect of fluoride toothpaste is not mediated primarily through effects on the plaque microflora, although effects on plaque physiology could be important.
authors.affiliation: Department of Microbiology, Guy's, King's and St. Thomas' Dental Institute, King's College London, London, UK.
authors.forename: S R
authors.initials: SR
authors.lastname: Brailsford
authors.forename: E A M
authors.initials: EA
authors.lastname: Kidd
authors.forename: S C
authors.initials: SC
authors.lastname: Gilbert
authors.forename: D T
authors.initials: DT
authors.lastname: Clark
authors.forename: D
authors.initials: D
authors.lastname: Beighton
mesh_terms: D000328:Adult; D002327:Cariostatic Agents / Q000627:therapeutic use*; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000188:drug therapy / Q000382:microbiology*; D004311:Double-Blind Method; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D008875:Middle Aged; D018709:Statistics, Nonparametric; D014100:Toothpastes / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D002327:Cariostatic Agents; D014100:Toothpastes; D005459:Fluorides