title: Genomic Comparison of cag pathogenicity island (PAI)-positive and -negative Helicobacter pylori strains: identification of novel markers for cag PAI-positive strains.
abstract: In an analysis of Helicobacter pylori genomic DNA by macroarray methodology, genomic DNA from a panel of cag pathogenicity island (PAI)-negative H. pylori clinical isolates failed to hybridize with 27 genes located outside the cag PAI in a cag PAI-positive reference strain. PCR analyses confirmed that HP0217 (encoding a lipopolysaccharide biosynthetic protein) and HP1079 (encoding a protein of unknown function) were present significantly more frequently in cagA-positive strains than in cagA-negative strains. A low G+C content of these two genes suggests they were acquired by horizontal transfer events.
authors.affiliation: Department of Biology, The College of William & Mary, 214 Millington Hall, Landrum Drive, Williamsburg, VA 23187-8795, USA.
authors.forename: Courtney E
authors.initials: CE
authors.lastname: Terry
authors.forename: Lisa M
authors.initials: LM
authors.lastname: McGinnis
authors.forename: Katharine C
authors.initials: KC
authors.lastname: Madigan
authors.forename: Ping
authors.initials: P
authors.lastname: Cao
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Cover
authors.forename: George W
authors.initials: GW
authors.lastname: Liechti
authors.forename: Richard M
authors.initials: RM
authors.lastname: Peek
authors.forename: Mark H
authors.initials: MH
authors.lastname: Forsyth
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015415:Biomarkers; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D044404:Genomic Islands; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; C080866:cagA protein, Helicobacter pylori