Gene expression profile and aortic vessel distensibility in voluntarily exercised spontaneously hypertensive rats: potential role of heat shock proteins.
Physical exercise is considered to be beneficial for cardiovascular health. Nevertheless, the underlying specific molecular mechanisms still remain unexplored. In this study, we aimed to investigate the effects of voluntary exercise on vascular mechanical properties and gene regulation patterns in spontaneously hypertensive rats. By using ultrasound biomicroscopy in an ex vivo perfusion chamber, we studied the distensibility of the thoracic aorta. Furthermore, exercise-induced gene regulation was studied in aortae, using microarray analysis and validated with real-time PCR. We found that distensibility was significantly improved in aortas from exercising compared with control rats (P < 0.0001). Exercising rats demonstrated a striking pattern of coordinated downregulation of genes belonging to the heat shock protein family. In conclusion, voluntary exercise leads to improved vessel wall distensibility and reduced gene expression of heat shock protein 60 and 70, which may indicate decreased oxidative stress in the aortic vascular wall.
Hägg
Ulrika
U
Department of Physiology, Institute of Physiology and Pharmacology, Göteborg University, Sweden.
Johansson
Maria E
ME
Grönros
Julia
J
Naylor
Andrew S
AS
Jonsdottir
Ingibjörg H
IH
Bergström
Göran
G
Svensson
Per-Arne
PA
Gan
Li-ming
LM
D000465:Algorithms; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D015233:Models, Statistical; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010477:Perfusion; D010805:Physical Conditioning, Animal; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018834:Chaperonin 60; D017931:DNA Primers; D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012313:RNA