title: Silica monolithic membrane as separation medium. Summable property of different membranes for high-performance liquid chromatographic separation.
abstract: We studied an applicability of a silica monolithic membrane as separation medium for high-performance liquid chromatography (HPLC). We prepared porous monolithic silica membranes having a three-dimensional network structure to cut and shape into a membrane separation medium. We evaluated chromatographic properties of a variety of solutes using a column containing the membranes with HPLC to elucidate summable property of the membrane separation media. In addition, we made brief study on separation of HbA1c in whole blood with the stacked" membranes having different surface characteristics in one column, which is a membrane column. We confirmed that the membrane column was able to separate HbA1c from other matrix in whole blood to some extent, and it also had an excellent ability for hydrophobic and ion exchange adsorption.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. kenpc@kit.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ogata
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Watabe
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Minakuchi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006442:Glycated Hemoglobin; D008567:Membranes, Artificial; D012822:Silicon Dioxide