title: Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation.
abstract: BACKGROUND
  The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis.
  
  
  METHODS
  A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores.
  
  
  RESULTS
  The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals.
  
  
  CONCLUSIONS
  This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.
authors.affiliation: Centro Studi sull'Invecchiamento (Ce.S.I.), Fondazione Università "G.D'Annunzio", Chieti, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Maddalo
authors.forename: Francesca
authors.initials: F
authors.lastname: Petrucci
authors.forename: Manuela
authors.initials: M
authors.lastname: Iezzi
authors.forename: Tania
authors.initials: T
authors.lastname: Pannellini
authors.forename: Piero
authors.initials: P
authors.lastname: Del Boccio
authors.forename: Domenico
authors.initials: D
authors.lastname: Ciavardelli
authors.forename: Antonino
authors.initials: A
authors.lastname: Biroccio
authors.forename: Federica
authors.initials: F
authors.lastname: Forlì
authors.forename: Carmine
authors.initials: C
authors.lastname: Di Ilio
authors.forename: Enzo
authors.initials: E
authors.lastname: Ballone
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
mesh_terms: D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D016014:Linear Models; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011471:Prostatic Neoplasms / Q000378:metabolism; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012468:Salivary Gland Neoplasms / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D020543:Proteome