[Study on identification of aconiti by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
OBJECTIVE
The merhod of determination of traditional Chinese medicine of aconitum root has been established by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.


METHODS
With optimized matrix of 2,5-Dihydroxy- benzoic acid for herbal extract, the identification of aconitum root by MALDI-TOF-MS has been conditioned. By comparison of mass spetra of various of aconitium root, their fingerprint mass-to-charge ratios were found to perform identification. With the characteristic mass-to-charge ratios, It was shown that the mass spectrum of raw aconitum roots were obviously different from that of processed aconitum roots.


RESULTS
The raw radix aconiti could be differentiated from radix aconiti preparate by determination of MALDI-TOF-MS.


CONCLUSIONS
The identifical method of aconitum root by MALDI-TOF-MS is rapid, precise and sensitive, it can be used in toxicological analysis of medical tangle and illegal medical practice.
Pan
Ai-hua
AH
Department of Forensic Toxicological Analysis, Preclinical School, Sun Yat-Sen University, Guangzhou 510089, China.
Liu
Jie
J
D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis*; D031841:Aconitum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D000431:Ethanol; D008752:Methylene Chloride; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013678:Technology, Pharmaceutical / Q000379:methods
D004365:Drugs, Chinese Herbal; D000431:Ethanol; D008752:Methylene Chloride; D000157:Aconitine