Cell-type-dependent activation of the cellular EF-1alpha promoter by the varicella-zoster virus IE63 protein.
The varicella-zoster virus (VZV) IE63 protein is abundantly expressed during productive viral infection and is one of six gene products that appear to be expressed during latency. We have found that the IE63 protein can activate expression from the cellular EF-1alpha promoter in the absence of other viral proteins. The VZV IE62 protein, in contrast, was not found to transactivate this promoter. These data indicate that IE63 can function independently of the IE62 protein to positively influence the cellular transcription apparatus. We show that IE63 activation of the EF-1alpha promoter is cell type dependent and have examined the effects of point mutations important for IE63 phosphorylation and virus viability on this activation.
Zuranski
Tricia
T
Department of Microbiology and Immunology and Witebsky Center for Microbial Pathogenesis and Immunology, 138 Farber Hall, University at Buffalo, Buffalo, NY 14214, USA.
Nawar
Hesham
H
Czechowski
Donna
D
Lynch
Jennifer M
JM
Arvin
Ann
A
Hay
John
J
Ruyechan
William T
WT
D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D020648:Peptide Elongation Factor 1; D015534:Trans-Activators; D014759:Viral Envelope Proteins; C074551:immediate early protein 63, Human herpesvirus 3