Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus.
Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
Jothikumar
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Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, 27599-7431, USA. JIN2@cdc.gov
Cromeans
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Sobsey
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Robertson
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BH
D020121:5' Untranslated Regions / Q000235:genetics*; D017931:DNA Primers; D004196:Disease Outbreaks; D004784:Environmental Monitoring / Q000379:methods; D062665:Epidemiological Monitoring; D006506:Hepatitis A / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D030041:Hepatitis A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism*; D013997:Time Factors
D020121:5' Untranslated Regions; D017931:DNA Primers; D012367:RNA, Viral; D019914:Taq Polymerase