title: Identification of an rRNA operon promoter from Zea mays chloroplasts which excludes the proximal tRNAValGAC from the primary transcript.
abstract: The transcriptional start site of the tRNA operon from Zea mays chloroplasts has ben identified by a combination of S1 mapping and Southern hybridization with in vitro capped chloroplast RNA as radioactive probe. This is the first example in which the transcriptional start site of a chloroplast gene has ben established by identification of the triphosphate-bearing RNA terminus. The start site is located at position -117 proximal to the 16S rRNA gene and is preceded by -10 and -35 sequences homologous to prokaryotic promoters. The primary transcript directed by this promoter does not include tRNAValGAC sequences which are coded further upstream between positions -302 and -231. A major processing site of the primary rRNA transcript was identified at position -30 which is embedded in a secondary structure typical for prokaryotic RNase III processing sites. Several putative processing and start sites of the tRNAValGAC transcripts have been mapped by primer extension with reverse transcriptase.
authors.affiliation: Insititut für Biologie III der Universität Freiburg, Schaenzlestrasse 1, D-7800 Freiburg, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Strittmatter
authors.forename: A
authors.initials: A
authors.lastname: Gozdzicka-Jozefiak
authors.forename: H
authors.initials: H
authors.lastname: Kössel
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D003313:Zea mays / Q000235:genetics*; D012407:rRNA Operon / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012346:RNA, Transfer, Amino Acyl