title: Clonality among Enterococcus faecium clinical isolates.
abstract: In the course of a survey to determine the epidemiology of enterococcal infections in Italy, a sudden increment, in a 1-year time, was noted in the number of glycopeptide resistant Enterococcus faecium isolated from different wards of the University Hospital in Rome, Italy. The isolates were characterized for clonal relatedness by comparing SmaI gel electropherotypes, presence of vancomycin-resistance genes, and expression of virulence factors. PFGE identified in a single pulsed type all the glycopeptide-resistant isolates but one. Resistance to high levels of aminoglycosides was expressed by these same isolates, which also included a majority of non biofilm-forming strains. Two esp gene-carrying strains were also identified in different PFGE types. Data indicates that a specific clone acquired, in the clinical setting, the genetic determinant for glycopeptide resistance, thus improving environmental adaptation and favoring its persistence and spread.
authors.affiliation: Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Università Campus Biomedico, Rome, Italy. baldassa@iss.it
authors.forename: L
authors.initials: L
authors.lastname: Baldassarri
authors.forename: L
authors.initials: L
authors.lastname: Bertuccini
authors.forename: R
authors.initials: R
authors.lastname: Creti
authors.forename: G
authors.initials: G
authors.lastname: Orefici
authors.forename: G
authors.initials: G
authors.lastname: Dicuonzo
authors.forename: G
authors.initials: G
authors.lastname: Gherardi
authors.forename: M
authors.initials: M
authors.lastname: Venditti
authors.forename: R
authors.initials: R
authors.lastname: Di Rosa
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics*; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans
chemical_list: D006020:Glycopeptides