title: Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent.
abstract: The development of a process intensified primary capture step for the direct selective recovery of intracellular proteins from very dense particulate-containing yeast extract has been explored. The purification of glyceraldehyde 3-phosphate dehydrogenase from bakers' yeast was chosen as a potential demonstration of this approach. A high throughput (50%, w/v, yeast extracts at a superficial linear velocity of 450 cm h(-1)) was achieved by adoption of a high-density adsorbent (UpFront steel-agarose; rho = 2.65 g ml(-1)) derivatized with selective ligand chemistries (Cibacron Blue 3GA). This should ultimately minimize adsorption time and maximize process efficiency of fluidized bed adsorption.
authors.affiliation: Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering, University of Birmingham, Edgbaston, UK. ltc555@eng.upm.edu.my
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
authors.forename: Andrew
authors.initials: A
authors.lastname: Lyddiatt
mesh_terms: D000465:Algorithms; D018533:Biomass; D002846:Chromatography, Affinity / Q000379:methods; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000302:isolation & purification*; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D013232:Steel / Q000737:chemistry
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C017842:cibacron blue-sepharose; D013232:Steel; D012685:Sepharose; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)