title: [No association between 3 polymorphisms of transforming growth factor beta3 gene and essential hypertension in Chinese].
abstract: OBJECTIVE
  To investigate possible association between the single nucleotide polymorphisms (SNPs) of transforming growth factor beta3 (TGF-beta3) gene and essential hypertension (EH) in Chinese.
  
  
  METHODS
  The promoter region, exons, as well as part of the introns of TGF-beta3 gene were sequenced by a fluorescent labeling automatic sequencing method to detect and characterize the SNPs in 24 DNA samples from a Chinese population. Then we conducted a case-control study using 396 patients with hypertension (case) and 214 nomortensive subjects (control). The three SNPs including Thr63Asn, SS5608219 and SS5608220 were genotyped by PCR-RFLP or real-time allele-specific PCR in subjects studied.
  
  
  RESULTS
  Seven SNPs in the exons, introns and 3'untranslated region (3'UTR) of TGF-beta3 gene were identified. Among them, 2 SNPs were found to be novel genetic variants and one of the two located in the exon 1 and produced substitution of amino acid. However, no differences were found between hypertensives and nomortensives in genotype distribution and allele frequency of SS5608219, Thr63Asn or SS5608220 polymorphisms.
  
  
  CONCLUSIONS
  Two novel SNPs of TGF-beta3 gene were identified in Chinese. One of them produces a threonine to asparagines substitution in codon 63 (Thr63Asn). But no association was found between TGF-beta3 gene polymorphisms and EH in Chinese.
authors.affiliation: Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Bang-Chuan
authors.initials: BC
authors.lastname: Hu
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Chu
authors.forename: Ji-Guang
authors.initials: JG
authors.lastname: Wang
authors.forename: Gu-Liang
authors.initials: GL
authors.lastname: Wang
authors.forename: Ping-Jin
authors.initials: PJ
authors.lastname: Gao
authors.forename: Ding-Liang
authors.initials: DL
authors.lastname: Zhu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005091:Exons; D005260:Female; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D053782:Transforming Growth Factor beta3 / Q000235:genetics*
chemical_list: C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3