Tumor necrosis factor alpha, CYP 17, urokinase, and interleukin 10 gene polymorphisms in postmenopausal women: correlation to bone mineral density and susceptibility to osteoporosis.
OBJECTIVE
Osteoporosis is a common disorder with a strong genetic component. We investigated the correlations between bone mineral density (BMD) and four gene polymorphisms (-308G>A tumor necrosis factor alpha (TNF-alpha), -34T>C CYP 17, *141T>C urokinase, and -627C>A interleukin 10 (IL-10) promoter), and their relationship to osteoporosis in postmenopausal women.


METHODS
These polymorphisms were determined using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis. BMD of the lumbar spine and proximal femur were measured using dual-energy X-ray absorptiometry.


RESULTS
The prevalence of each genotype was as follows: (1) 79.3% A/A, 16.6% A/G, and 4.1% G/G in -308G>A TNF-alpha; (2) 18.9% T/T, 52.1% T/C, and 29% C/C in -34T>C CYP 17; (3) 86.4% C/C and 13.6% C/T in *141T>C urokinase; (4) 46.2% A/A, 45% A/C, and 8.8% C/C in -627C>A IL-10 promoter. Subjects with genotype C/C in -627C>A IL-10 promoter had lower BMD values and a significantly greater risk for osteoporosis (OR 8.1, 95% CI 1.5-42.8) at the lumbar spine compared with subjects with genotype A/C in -627C>A IL-10 promoter, after adjustment for potential confounders.


CONCLUSIONS
The RsaI IL-10 promoter gene polymorphism is associated with reduced BMD and predisposes women to osteoporosis at the lumbar spine.
Chen
Huey-Yi
HY
Department of Obstetrics and Gynecology, China Medical University Hospital, School of Medicine, China Medical University, No. 2, Yu-Der Road, Taichung 404, Taiwan.
Chen
Wen-Chi
WC
Hsu
Chin-Moo
CM
Tsai
Fuu-Jen
FJ
Tsai
Chang-Hai
CH
D015502:Absorptiometry, Photon; D015519:Bone Density; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics
D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D013254:Steroid 17-alpha-Hydroxylase; D014568:Urokinase-Type Plasminogen Activator