[Study of the effects of immunosuppressive agents on human islet cells in vitro].
OBJECTIVE
To investigate the effects of immunosuppressive agents on insulin secretion of human islet cells in vitro.


METHODS
Human islet cells were isolated by the solution of the liberase and purified by Ficoll's density gradient centrifugation and then were exposed to various concentrations of four immunosuppressive agents for 24 hr respectively. Glucose-stimulated insulin secretion during subsequent static incubation was measured using the human insulin ELISA kit.


RESULTS
Glucose-stimulated insulin secretion from human islet cells was significantly reduced after exposed to high concentrations of MMF and FK506 (both P < 0.05). No significant reduction in insulin secretion was observed from human islet cells after exposed to FTY720 and rapamycin (both P > 0.05).


CONCLUSIONS
(1) High concentrations of MMF and FK506 have deleterious effects on insulin secretion in human islet cells. Low-dose FK506 + MMF is available for clinical use. (2) FTY720 and rapamycin have no adverse effects on insulin secretion in human islet cells. FTY720 and rapamycin may become useful immunosupressants for future clinical islet allotransplantation.
Cai
Jin-Quan
JQ
Institute of Organ Transplantation, Fuzhou General Hospital, Fuzhou 350025, China.
Tan
Jian-Ming
JM
Dong
Wei-Ping
WP
Wang
Yu-Fei
YF
Wang
Jian-Bo
JB
D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000068876:Fingolimod Hydrochloride; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology*; D011409:Propylene Glycols / Q000494:pharmacology*; D020123:Sirolimus / Q000494:pharmacology*; D013110:Sphingosine / Q000031:analogs & derivatives; D016559:Tacrolimus / Q000494:pharmacology
D007166:Immunosuppressive Agents; D011409:Propylene Glycols; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine; D020123:Sirolimus; D016559:Tacrolimus