Supercritical fluid chromatography tandem-column method development in pharmaceutical sciences for a mixture of four stereoisomers.
A tandem-column method using Chiralpak AD-H and Chiralcel OD-H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i.e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% ethanol:isopropanol (50:50 v/v). On the contrary, this mixture (mixture A) could not be baseline separated by SFC conditions explored with individual Chiralpak AD-H and Chiralcel OD-H columns. The effects of various mobile phases on elution order, capacity factor, selectivity, and resolution were determined with mixture A on the individual aforementioned columns to develop the tandem-column method.
Barnhart
Wesley W
WW
Discovery Analytical Sciences, Molecular Structure, Amgen Inc., Thousand Oaks, CA 91320, USA. wesleyb@amgen.com
Gahm
Kyung H
KH
Thomas
Sam
S
Notari
Steve
S
Semin
David
D
Cheetham
Janet
J
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013237:Stereoisomerism; D013678:Technology, Pharmaceutical / Q000379:methods*
D004364:Pharmaceutical Preparations