A note concerning acetate activation of peroxidative activity of catalases using 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid as a substrate.
Beef liver catalases showed peroxidative activity using 2,2'-azino-bis-(3-ethylbenzthiazoline)-6-sulfonic acid as the electron donor and hydrogen peroxide as the acceptor at a pH of 5. This activity was not observed at pH 7. The reaction depended on acetate concentration, although succinate and propionate could partly replace the acetate as a catalyst. Other haem proteins also catalyzed a peroxidative effect. The reaction using syringaldazine or the coupling between dimethylaminobenzoic acid and 3-methyl-2-benzothiazolinone hydrazone was less effective and less sensitive. Evidence is presented that the reaction is associated with a conformational change of the catalase.
Baker
Warren L
WL
Environment and Biotechnology Centre, Swinburne University of Technology, John Street, Hawthorn, 3122, Melbourne, Australia. wbaker@swin.edu.au
Key
Christopher
C
Lonergan
Greg T
GT
D000818:Animals; D052160:Benzothiazoles; D002374:Catalase / Q000737:chemistry*; D002417:Cattle; D004789:Enzyme Activation; D004795:Enzyme Stability; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry*
D052160:Benzothiazoles; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D002374:Catalase