title: The structure of the capsular polysaccharide of Shewanella oneidensis strain MR-4.
abstract: Capsular polysaccharides were extracted from Shewanella oneidensis strain MR-4, grown on two different culture media. The polysaccharides were analyzed using 1H and 13C NMR spectroscopy, and the following structure of the repeating unit was established: [structure: see text] where the residue of 4-amino-4,6-dideoxy-D-glucose (Qui4N) was substituted with different N-acyl groups depending on the growth media. All monosaccharides are present in the pyranose form. In the PS from cells grown on enriched medium (trypticase soy broth, TSB) aerobically it was N-acylated with 3-hydroxy-3-methylbutyrate (60%) or with 3-hydroxybutyrate (40%), whereas in the PS from cells grown on minimal medium (CDM) aerobically it was acylated mostly with 3-hydroxybutyrate (>90%).
authors.affiliation: Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. evguenii.vinogradov@nrc.ca
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Ludmila
authors.initials: L
authors.lastname: Nossova
authors.forename: Anton
authors.initials: A
authors.lastname: Korenevsky
authors.forename: Terry J
authors.initials: TJ
authors.lastname: Beveridge
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D020592:Shewanella / Q000737:chemistry* / Q000145:classification*
chemical_list: D011135:Polysaccharides, Bacterial