Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays.
A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.
Pereira
A
A
Max-Planck-Institut fur Züchtungsforschung, 5000 Köln 30, FRG.
Schwarz-Sommer
Z
Z
Gierl
A
A
Bertram
I
I
Peterson
P A
PA
Saedler
H
H
D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D017353:Gene Deletion; D017343:Genes, Plant / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements