CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.
The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
Mustonen
Harri
H
Department of Surgery, Helsinki University Central Hospital, Finland. harri.mustonen@helsinki.fi
Lepistö
Anna
A
Lehtonen
Sanna
S
Lehtonen
Eero
E
Puolakkainen
Pauli
P
Kivilaakso
Eero
E
D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D005753:Gastric Mucosa / Q000502:physiology*; D051381:Rats
D033942:Adaptor Proteins, Vesicular Transport; D015820:Cadherins