title: Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection.
abstract: A sensitive and accurate analytical method for the determination of ochratoxin A (OTA) in rice, based on extraction with phosphate-buffered saline/methanol, an immunoaffinity column (IAC) for clean-up, and high performance liquid chromatography with fluorescence detection (HPLC-FD), is described. The limit of quantification of the proposed method was 0.05 microg kg(-1). Recovery of OTA from rice samples spiked at 0.05 microg kg(-1) was 92%, with a within-day RSD of 5.4%. The proposed method was applied to 42 rice samples from Portugal and the presence of OTA was found in six samples at concentrations ranging from 0.09 to 3.52 microg kg(-1). The identification of OTA was confirmed by methyl ester derivatization and then HPLC analysis. The daily intake of OTA by the Portuguese population was also estimated.
authors.affiliation: Group of Bromatology, Faculty of Pharmacy, University of Coimbra, 3049 Coimbra, Portugal. apena@ci.uc.pt
authors.forename: A
authors.initials: A
authors.lastname: Pena
authors.forename: F
authors.initials: F
authors.lastname: Cerejo
authors.forename: C
authors.initials: C
authors.lastname: Lino
authors.forename: I
authors.initials: I
authors.lastname: Silveira
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D011174:Portugal; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A