title: Partial structure of the phylloxin gene from the giant monkey frog, Phyllomedusa bicolor: parallel cloning of precursor cDNA and genomic DNA from lyophilized skin secretion.
abstract: Phylloxin is a novel prototype antimicrobial peptide from the skin of Phyllomedusa bicolor. Here, we describe parallel identification and sequencing of phylloxin precursor transcript (mRNA) and partial gene structure (genomic DNA) from the same sample of lyophilized skin secretion using our recently-described cloning technique. The open-reading frame of the phylloxin precursor was identical in nucleotide sequence to that previously reported and alignment with the nucleotide sequence derived from genomic DNA indicated the presence of a 175 bp intron located in a near identical position to that found in the dermaseptins. The highly-conserved structural organization of skin secretion peptide genes in P. bicolor can thus be extended to include that encoding phylloxin (plx). These data further reinforce our assertion that application of the described methodology can provide robust genomic/transcriptomic/peptidomic data without the need for specimen sacrifice.
authors.affiliation: School of Pharmacy, Queen's University, Lisburn Road, Belfast BT9 7BL Northern Ireland, UK.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Chen
authors.forename: Ron
authors.initials: R
authors.lastname: Gagliardo
authors.forename: Brian
authors.initials: B
authors.lastname: Walker
authors.forename: Mei
authors.initials: M
authors.lastname: Zhou
authors.forename: Chris
authors.initials: C
authors.lastname: Shaw
mesh_terms: D029845:Amphibian Proteins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D001001:Anura / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000378:metabolism
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger; C404703:phylloxin; C070905:dermaseptin