title: Sequence dependence of tRNA(Gly) import into tobacco mitochondria.
abstract: Plant mitochondrial genomes lack a number of tRNA genes and the corresponding tRNAs, which are nuclear-encoded, are imported from the cytosol. We show that specific import of tRNA(Gly) isoacceptors occurs in tobacco mitochondria: tRNA(Gly)(UCC) and tRNA(Gly)(CCC) are cytosolic and mitochondrial, while tRNA(Gly)(GCC) is found only in the cytosol. Exchange of sequences between tRNA(Gly)(UCC) and tRNA(Gly)(GCC) shows that the anticodon and D-domain are essential for tRNA(Gly)(UCC) import. However the reverse mutations in tRNA(Gly)(GCC) are not sufficient to promote its import into tobacco mitochondria.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR du CNRS no. 2357, Université Louis Pasteur, 12, rue du Général Zimmer, 67000 Strasbourg, France.
authors.forename: Thalia
authors.initials: T
authors.lastname: Salinas
authors.forename: Cécile
authors.initials: C
authors.lastname: Schaeffer
authors.forename: Laurence
authors.initials: L
authors.lastname: Maréchal-Drouard
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Duchêne
mesh_terms: D000926:Anticodon; D001483:Base Sequence; D003600:Cytosol / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation; D030821:Plants, Genetically Modified; D034443:RNA Transport / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000737:chemistry / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D046249:Transfer RNA Aminoacylation
chemical_list: D000926:Anticodon; D012353:RNA, Transfer, Gly