title: Microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (PHAs) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in Hong Kong.
abstract: The first objective of this study was the measurement of physical properties of P(3HB-co-3HV) copolymers with different (hydroxybutyrate) HB to (hydroxyvalerate) HV ratios produced by Bacillus cereus (TRY2) isolated from activated sludge. The 3HV PHBV copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. The second objective was to study possible waste water treatment and production of PHAs at the same time by B. cereus (TRY2) and Pseudomonas spp. (TOB17) (both were isolated from activated sludge), recombinant Bacillus DH5alpha, and a combination of the above three bacteria. The results were satisfactory; the maximum COD and TOC of the sewage sludge reduced were 53.5% and 67.5%, respectively.
authors.affiliation: State Key Lab of Chinese Med and Molecular Pharmacology, Shenzen, China.
authors.forename: Tsz-Chun
authors.initials: TC
authors.lastname: Ma
authors.forename: P L
authors.initials: PL
authors.lastname: Chan
authors.forename: H
authors.initials: H
authors.lastname: Lawford
authors.forename: H
authors.initials: H
authors.lastname: Chua
authors.forename: W H
authors.initials: WH
authors.lastname: Lo
authors.forename: Peter Hoifu
authors.initials: PH
authors.lastname: Yu
mesh_terms: D001409:Bacillus cereus / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004548:Elasticity; D005240:Feasibility Studies; D006885:Hydroxybutyrates / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control; D008970:Molecular Weight; D011091:Polyesters / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods; D012722:Sewage / Q000382:microbiology*; D013718:Tensile Strength; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D007220:Industrial Waste; D011091:Polyesters; D012722:Sewage; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate