title: Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea.
abstract: From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in 2002 formed one distinct cluster, which different from clusters of Korean isolates in 2000, with in the same lineage of the ME-SA topotype strains. Deduced amino acid sequences around neutralizable antigenic site on VP1 site of O/SKR/2002 isolates were aligned and compared with other strains. At the antigenic site 1, the replacements of the critical amino acid residues at position 144 from V to L and at position 152 from A to T were observed in O/SKR/2002 viruses. For antigenic site 2 and 4, there were not significant variations in general. At the antigenic site 3, the substitutions of amino acid residues were present at positions 54 and 56 in O/SKR/2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O/SKR/AS/2002 (cow) virus. And the substitution (L-->P) of significant residue at position 144 was detected at the amino acid sequence of the O/SKR/2002 (cow) virus.
authors.affiliation: National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Korea. jku0622@nvrqs.go.kr
authors.forename: Jae Ku
authors.initials: JK
authors.lastname: Oem
authors.forename: Kwang Nyeong
authors.initials: KN
authors.lastname: Lee
authors.forename: In Soo
authors.initials: IS
authors.lastname: Cho
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Kye
authors.forename: Jee Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Jong Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Yong Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Yi Seok
authors.initials: YS
authors.lastname: Joo
authors.forename: Hee Jong
authors.initials: HJ
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D016000:Cluster Analysis; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000032:analysis; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D012367:RNA, Viral; C442501:VP1 protein, Foot-and-mouth disease virus