Chiral separation of tamsulosin by capillary electrophoresis.
Enantiomers of (+/-) 5-[2 (R,S)-{[2-(o-ethoxyphenoxy) ethyl] amino} propyl]-2-methoxy-benzenesulfonamide (tamsulosin, drug frequently used in the treatment of prostate diseases) were separated by capillary electrophoresis (CE). An acidic background electrolyte (BGE) with sulfated-beta-cyclodextrin (S-beta-CD) was used to create a chiral separation environment. Baseline separation of the isomers was achieved during 5 min using cathodic electro-osmotic flow (EOF) (countercurrent mode). The quantification limits were 5.3 x 10(-6) moll(-1) for R-isomer and 5.7 x 10(-6) moll(-1) for S-isomer. The R.S.D. values of peak area were 0.54% for R-isomer and 0.75% for S-isomer. The results achieved enable determination of 0.5% of optical impurity.
Maier
Vítezslav
V
Department of Analytical Chemistry, Palacký University, Trída Svobody 8, CZ-77146 Olomouc, Czech Republic. maierv@email.cz
Horáková
Jana
J
Petr
Jan
J
Tesarová
Eva
E
Coufal
Pavel
P
Sevcík
Juraj
J
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000032:analysis; D000317:Adrenergic alpha-Antagonists / Q000032:analysis / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes; D004586:Electrophoresis; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005663:Furans / Q000032:analysis / Q000737:chemistry; D008956:Models, Chemical; D009995:Osmosis; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013449:Sulfonamides / Q000032:analysis / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry; D000077409:Tamsulosin; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000032:analysis / Q000737:chemistry*
D000097:Acetonitriles; D000317:Adrenergic alpha-Antagonists; D004573:Electrolytes; D005663:Furans; D013449:Sulfonamides; D047392:beta-Cyclodextrins; C018674:tetrahydrofuran; D013455:Sulfur; D000077409:Tamsulosin; C031215:betadex; D019342:Acetic Acid; C032159:acetonitrile