[Association of XRCC1 polymorphisms and chromosomal damage levels in coke-oven workers].
OBJECTIVE
To investigate the association of XRCC1 polymorphisms and chromosomal damage levels in peripheral blood lymphocyte in coke-oven workers.


METHODS
The study included 141 coke-oven workers who exposed to a high level of polycyclic aromahaplotpetic hydrocarbon and 66 non-exposed controls. Urinary 1-hydroxypyrene and chromosome damage in peripheral lymphocyte were measured. Four -tagging single nucleotide polymorphisms in XRCC1 gene, including C26304T, G27466A, G28152A and G36189A, were detected and the XRCC1 haplotypes were estimated by using an extension of Clark algorithm. The associations between haplotype pairs and micronuclei data were assessed by analysis of covariance in the exposed and non-exposed groups.


RESULTS
The geometric means of urinary 1-hydroxypyrene levels in coke-oven workers and the controls were 12.0 and 0.7 micromol/mol Cr respectively (P < 0.01). The cytokinesis-block micronucleus cytokinesis-block micronucleus frequencies (number of micronucleus per 1 000 binucleated lymphocytes) was significantly higher in coke-oven workers (0.95 +/- 0.66)% than in the controls (0.40 +/- 0.36)%, P < 0.01. The haplotype CGGG was associated with the decreased frequencies of total micronucleus, and the haplotypes TGGG (P = 0.01) and CGAG (P < 0.05) were associated with the increased frequencies of total micronucleus in the multivariate analysis with adjustment for covariates among coke-oven workers.


CONCLUSIONS
The genetic polymorphisms of XRCC1 gene could influence the chromosome damage levels in coke-oven workers.
Leng
Shu-Guang
SG
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Cheng
Juan
J
Zhang
Lin-Yuan
LY
Pan
Zu-Fei
ZF
Dai
Yu-Fei
YF
Niu
Yong
Y
Sun
Yao-Feng
YF
Li
Bin
B
He
Feng-Sheng
FS
Zheng
Yu-Xin
YX
D000328:Adult; D002681:China; D019457:Chromosome Breakage; D003077:Coke / Q000506:poisoning*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D008875:Middle Aged; D009784:Occupational Diseases / Q000209:etiology / Q000235:genetics / Q000652:urine; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000032:analysis; D000076105:X-ray Repair Cross Complementing Protein 1
D003077:Coke; D004268:DNA-Binding Proteins; D011721:Pyrenes; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C033146:1-hydroxypyrene