title: [Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district].
abstract: OBJECTIVE
  To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.
  
  
  METHODS
  Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package.
  
  
  RESULTS
  A 278 base pairs region of the gene for CO I encoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species.
  
  
  CONCLUSIONS
  It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the CO I encoding gene of mtDNA.
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. cjf_jifeng@163.com
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Cai
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Dong
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods; D007814:Larva / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011679:Pupa / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV