Effect of ginsenoside Rb1 and compound K in chronic oxazolone-induced mouse dermatitis.
During the screening program to discover antipsoriatic agents from natural products, ginseng was found to show inhibitory activity in oxazolone-induced mouse ear dermatitis. Therefore, the effects of a main constituent ginsenoside Rb1 isolated from ginseng and its metabolite compound K on oxazolone-induced mouse ear dermatitis were investigated. Compound K at concentrations of 0.02% and 0.05% also potently suppressed mouse ear swelling by 54% and 76% at 16 days, respectively, although ginsenoside Rb1 did not significantly show the inhibitory activity. The compound K also significantly reduced the levels of mRNA of cyclooxygenase (COX)-2, IL-1beta, TNF-alpha, IFN-gamma and IL-4 increased in oxazolone-applied mouse ears. Based on these findings, the compound K may improve contact dermatitis or psoriasis by the regulation of COX-2 produced by macrophage cells and interferon-gamma and IL-4 induced by Th cells.
Shin
Yong-Wook
YW
College of Pharmacy, Kyung Hee University, 1, Hoegi, Dongdaemun-ku, Seoul 130-701, South Korea.
Bae
Eun-Ah
EA
Kim
Sung-Soo
SS
Lee
Young-Chul
YC
Kim
Dong-Hyun
DH
D000818:Animals; D002460:Cell Line; D002908:Chronic Disease; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D003877:Dermatitis, Contact / Q000188:drug therapy*; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D036145:Ginsenosides / Q000627:therapeutic use*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D010081:Oxazolone / Q000633:toxicity*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D011565:Psoriasis / Q000188:drug therapy*; D012333:RNA, Messenger / Q000032:analysis
D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D036145:Ginsenosides; D008565:Membrane Proteins; D009569:Nitric Oxide; D010081:Oxazolone; D011451:Prostaglandin-Endoperoxide Synthases; D012333:RNA, Messenger; C442759:ginsenoside Rb1; C112772:ginsenoside compound K; C496512:Ptgs1 protein, mouse