Sugarcane bagasse as raw material and immobilization support for xylitol production.
Xylose-to-xylitol bioconversion was performed utilizing Candida guilliermondii immobilized in sugarcane bagasse and cultured in Erlenmeyer flasks using sugarcane bagasse hydrolysate as the source of xylose. Fermentations were carried out according to a factorial design, and the independent variables considered were treatment, average diameter, and amount of bagasse used as support for cell immobilization. By increasing the amount of support, the xylitol yield decreased, whereas the biomass yield increased. The diameter of the support did not influence xylitol production, and treatment of the bagasse with hexamethylene diamine prior to fermentation resulted in the highest amount of immobilized cells.
Santos
Júlio C
JC
Faculdade de Engenharia Química de Lorena, Departamento de Biotecnologia, Rodovia Itajubá-Lorena, Km 74.5, C.P. 116, CEP, 12.600-970, Lorena/SP, Brazil.
Pinto
Icaro R G
IR
Carvalho
Walter
W
Mancilha
Ismael M
IM
Felipe
Maria G A
MG
Silva
Silvio S
SS
D001711:Biotransformation; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D012037:Refuse Disposal / Q000379:methods; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism*; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism*
D007220:Industrial Waste; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D014993:Xylitol