title: [Primary study on the influence of blood transfusion on the STR genotypes of recipient's blood].
abstract: OBJECTIVE
  To investigate whether any change occurred in recipients' blood collected at different times after transfusion with different quantity of blood.
  
  
  METHODS
  Three patients were transfused with 400 ml, 800 ml and 1200 ml blood separately. The blood samples were collected from the recipients before transfusion and at 4 h, 8 h, 12 h after transfusion, and from the donors. DNA were extracted by Chelex-100 method and were amplified by the polymerase chain reaction (PCR). Six loci of D1S549, D18S865, D3S1754, D12S391, D12S375, D6S477 were selected. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis and silver staining.
  
  
  RESULTS
  The investigations on the six STR loci revealed that the patients' STR genotypes remained unchanged within 12 h after blood transfusion.
  
  
  CONCLUSIONS
  The STR genotypes of the donors would have no influence on the STR genotypes of the patients within 12 h after transfusion providing the volume of blood transfused is less than 1200 ml.
authors.affiliation: Department of Forensic Biology, West China School of Preclincal and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-ge
authors.initials: JG
authors.lastname: Han
authors.forename: Guo-di
authors.initials: GD
authors.lastname: Chen
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.forename: Miao
authors.initials: M
authors.lastname: Liao
authors.forename: Mei-yun
authors.initials: MY
authors.lastname: Wu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Kang
authors.forename: Chuan-xin
authors.initials: CX
authors.lastname: Luo
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Jin
mesh_terms: D044466:Asian People; D001803:Blood Transfusion; D004247:DNA / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D004247:DNA