title: Enzymes responsible for chlorate reduction by Pseudomonas sp. are different from those used for perchlorate reduction by Azospira sp.
abstract: Pseudomonas sp. PDA is an unusual bacterium due to its ability to respire using chlorate under aerobic conditions. The chlorate reductase produced by PDA was shown to be intrinsically different from the enzyme responsible for chlorate and perchlorate [(per)chlorate] reduction produced by Azospira sp. KJ based on subunit composition and other enzyme properties. The perchlorate reductase from strain KJ appeared to have two subunits (100 and 40 kDa) while the chlorate reductase from PDA had three subunits (60, 48, and 27 kDa). N-terminal amino acid sequencing of the 100 kDa protein from strain KJ showed a 77% similarity with the perchlorate reductase alpha subunit from another perchlorate-respiring bacterium, Dechloromonas agitata, while the N-terminus amino acid sequence of the 60 kDa protein from strain PDA did not show a similarity to previously isolated chlorate or perchlorate reductases.
authors.affiliation: Department of Civil and Environmental Engineering, 212 Sackett Building, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Steinberg
authors.forename: John J
authors.initials: JJ
authors.lastname: Trimble
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Logan
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002704:Chlorates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism*; D021122:Protein Subunits; D011549:Pseudomonas / Q000201:enzymology*; D043362:Rhodocyclaceae / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002704:Chlorates; D010472:Perchlorates; D021122:Protein Subunits; D010088:Oxidoreductases; C021777:chlorate reductase