title: Cytochrome p4501a induced differentially in endothelial cells cultured from different organs of Anguilla rostrata.
abstract: Endothelial cells are a structural barrier and an active regulator of many bodily processes. Cytochrome P4501A (CYP1A) activity is induced in the endothelium of teleosts and mammals exposed to lipophilic xenobiotics, such as polycyclic aromatic hydrocarbons, and can have significant consequences for endothelial functions. We exposed cultures of characterized endothelial cells from the heart, kidney, and rete mirabile of the eel, Anguilla rostrata, to aryl hydrocarbon receptor (AhR) agonists. In heart endothelial cells, the maximum response (based on O-deethylation of 7-ethoxyresorufin to resorufin [EROD] activity) to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 113 pmol/mg/min, was at 1 nM TCDD and the peak response to beta-napthoflavone (betaNF), 135 pmol/mg/min, was at 3 microM betaNF. The maximum response to TCDD in the kidney endothelial cells is 12 pmol/mg/min at 0.3 nM TCDD. The rete mirabile capillary endothelial cells responded minimally or not at all to exposure to TCDD and betaNF. Both the heart and kidney endothelial cells (but not the rete mirabile capillary cells) have a low level of EROD activity (12.7 and 5.2 pmol/mg/min, respectively) in untreated or dimethylsulfoxide-treated cells. The robust response of the heart endothelial cells to induction and the lack of response in the rete mirabile capillary endothelial cells indicate that these cells are a good resource to use to investigate the physiological consequences of AhR agonist exposure and CYP1A induction in different areas of the vasculature.
authors.affiliation: Department of Natural Sciences, Fordham University, 113 W. 60th Street, New York, New York 10023, USA. rgarrick@whoi.edu
authors.forename: Rita Anne
authors.initials: RA
authors.lastname: Garrick
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Woodin
authors.forename: John J
authors.initials: JJ
authors.lastname: Stegeman
mesh_terms: D000811:Anguilla; D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D042783:Endothelial Cells / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D007668:Kidney / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D009928:Organ Specificity; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D018336:Receptors, Aryl Hydrocarbon / Q000037:antagonists & inhibitors*; D019324:beta-Naphthoflavone / Q000633:toxicity
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1