Neuronal specific regulatory elements in apolipoprotein E gene proximal promoter.
Systemic and local changes in apolipoprotein E (ApoE) quantity have been related with Alzheimer's and other neurodegenerative diseases, showing the relevance of maintaining physiological ApoE levels. However, APOE transcription has not been extensively studied in neural cells. In this report, we study the transcriptional activity of different APOE proximal promoter regions and their binding to nuclear proteins from human neural (astrocytoma and neuroblastoma) and peripheral (hepatoma and lymphoma) cell lines. We define several regions with a negative regulatory effect in all the cells and a region with a strong positive activity in neuroblastoma cells. Additionally, we show that the -219T/G polymorphism produces variations in APOE transcriptional activity, with the G allele showing higher activity.
Ramos
María C
MC
Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Matías
Salomón
S
Artiga
María J
MJ
Pozueta
Julio
J
Sastre
Isabel
I
Valdivieso
Fernando
F
Bullido
María J
MJ
D001057:Apolipoproteins E / Q000235:genetics*; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
D001057:Apolipoproteins E