title: Evaluation of biphasic culture system for mycobacterial isolation from the sputum of patients with pulmonary tuberculosis.
abstract: Mycobacterial diseases continue to cause high morbidity and mortality. Isolation, identification and sensitivity testing form the backbone of laboratory investigations. M. tuberculosis isolation needs 6-8 weeks on conventional egg containing media. For rapid isolation various methods have been evaluated. We evaluated biphasic system (Middlebrook 7H11 agar slant+Middlebrook 9H broth) in comparison with Lowenstein-Jensen (LJ) medium. In smear positive cases biphasic system showed the recovery rate of 97.05% as against 79.41% on LJ on incubation for 21+/-4.44 and 28+/-3.76 days respectively. In smear negative and culture positive cases biphasic system and LJ showed isolation rates of 91.66% and 66.6% after 36+/-3.44 and 41+/- 4.09 days respectively. Biphasic system showed lower contamination rate (1.33%). Biphasic medium is superior to LJ medium in isolation of M. tuberculosis.
authors.affiliation: Department of Microbiology, Dr. V. M. Medical College, Solapur, India. mangalghatole@hotmail.com
authors.forename: M
authors.initials: M
authors.lastname: Ghatole
authors.forename: C
authors.initials: C
authors.lastname: Sable
authors.forename: P
authors.initials: P
authors.lastname: Kamale
authors.forename: S
authors.initials: S
authors.lastname: Kandle
authors.forename: V
authors.initials: V
authors.lastname: Jahagirdar
authors.forename: V
authors.initials: V
authors.lastname: Yemul
mesh_terms: D000362:Agar; D015169:Colony Count, Microbial / Q000379:methods*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000362:Agar