Comparative study of four extraction methods for enterovirus recovery from wastewater and sewage sludge.
This study investigated four methods for the recovery of enteroviruses from sterilized raw wastewater, activated sludge, thickened sludge and treated wastewater, inoculated with Echovirus 11, Gregory prototype. The adsorption-elution method recommended by the US Environmental Protection Agency (EPA) was better for Echovirus 11 recovery than a sonication method, a modified EPA method and a membrane adsorption elution method since it resulted in the highest detection levels by cell culture and RT-PCR (Friedman's test, p<0.00041 and p=0.041, respectively).
Belguith
Khaoula
K
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie de Monastir, 5000 Rue Avicenne, Monastir, Tunisia.
Hassen
Abdennaceur
A
Aouni
Mahjoub
M
D002478:Cells, Cultured; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000821:virology*; D014776:Virus Cultivation; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
D012722:Sewage