title: TCP10L is expressed specifically in spermatogenic cells and binds to death associated protein kinase-3.
abstract: The human transcriptional factor T-complex protein 10 like (TCP10L) gene is expressed exclusively in the liver and testis. However, the function of TCP10L in the testis remains unknown. We examined the expression of TCP10L in human testis and found that TCP10L was expressed specifically in the nucleus of spermatogenic cells during spermatogenesis. In addition, we identified death associated protein kinase 3 (DAPK-3/ZIP kinase) as a binding partner for TCP10L by yeast two-hybrid screening, followed with immunoprecipitation and subcellular localization experiments. Mutagenesis study revealed that this interaction was dependant on the leucine zipper motif-containing region. The specific expression pattern of TCP10L and interaction with DAPK-3 implies that TCP10L might play crucially important roles in spermatogenesis through its interaction with DAPK-3.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Yu
authors.forename: Daojun
authors.initials: D
authors.lastname: Jiang
authors.forename: Zekun
authors.initials: Z
authors.lastname: Guo
authors.forename: Hexige
authors.initials: H
authors.lastname: Saiyin
authors.forename: Jinhu
authors.initials: J
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D018929:Cell Culture Techniques; D002460:Cell Line; D017931:DNA Primers; D064550:Death-Associated Protein Kinases; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013091:Spermatogenesis; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000502:physiology; D014162:Transfection; D056427:t-Complex Genome Region
chemical_list: D051017:Apoptosis Regulatory Proteins; D017931:DNA Primers; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C579101:DAPK3 protein, human; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases