Comparison of PCR-DGGE and selective plating methods for monitoring the dynamics of a mixed culture population in synthetic brewery wastewater.
Enrichment of an activated sludge inoculum in synthetic brewery wastewater, which included glucose, maltose, and ethanol, was conducted in batch experiments to identify the dominant microbes present, to determine methodologies capable of monitoring the mixed culture population dynamics, and to determine the consortium's substrate degradation behavior. These results and methodologies were subsequently used in the determination of the population dynamics of suspended and attached microorganisms in a sequencing batch system in the second part of this research work. The three-membered microbial community comprised two bacterial and one fungal species that were identified as Acinetobacter sp., Enterobacter sp., and Candida sp. PCR-DGGE and plating on selective media were used to track the population dynamics of the consortium during the degradation of different substrates in synthetic wastewater containing glucose, maltose, and ethanol. Enterobacter sp. could degrade glucose and maltose but not ethanol, whereas Acinetobacter and Candida could degrade all three carbon sources. In buffered batch mixed culture experiments, Enterobacter was the predominant bacterium until the sugar concentrations decreased to levels that enabled Acinetobacter and Candida to degrade ethanol. PCR-DGGE was effective for detecting the dominant species, but culture-based methods were more accurate for monitoring the population dynamics of these microorganisms during growth in the wastewater medium.
Tam
Kawai
K
Department of Chemical and Environmental Engineering and Department of Environmental Sciences, University of California, Riverside, California 92521, USA.
Yang
Ching-Hong
CH
Matsumoto
Mark R
MR
Crowley
David E
DE
Sheppard
John D
JD
D000150:Acinetobacter / Q000302:isolation & purification* / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology; D002175:Candida / Q000302:isolation & purification* / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D015169:Colony Count, Microbial / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D004754:Enterobacter / Q000302:isolation & purification* / Q000502:physiology; D007220:Industrial Waste / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics
D007220:Industrial Waste; D014874:Water Pollutants, Chemical