Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis.
RNA interference (RNAi), a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), can be used not only as a research tool but also as a therapeutic strategy for viral infection. We demonstrated that intracellular expression of short hairpin RNA (shRNA) targeting human cyclin T1 (hCycT1), a cellular factor essential for transcription of messenger and genomic RNAs from the long terminal repeat promoter of provirus of human immunodeficiency virus type 1 (HIV-1), could effectively suppress the replication of HIV-1. We also showed that downregulation of hCycT1 did not cause apoptotic cell death, therefore, targeting cellular factor hCycT1 by shRNAs may provide an attractive approach for genetic therapy of HIV-1 infection in the future.
Li
Zhaoyang
Z
The Modern Virology Research Centre and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Xiong
Yong
Y
Peng
Yu
Y
Pan
Ji'an
J
Chen
Yu
Y
Wu
Xiaoyun
X
Hussain
Snawar
S
Tien
Po
P
Guo
Deyin
D
D017209:Apoptosis; D002460:Cell Line; D056906:Cyclin T; D016213:Cyclins / Q000172:deficiency* / Q000235:genetics*; D015536:Down-Regulation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014779:Virus Replication
C503684:CCNT1 protein, human; D056906:Cyclin T; D016213:Cyclins; D012313:RNA