title: Identification and cornification-related gene expression of canine keratinocyte differentiation-associated protein, Kdap.
abstract: The outermost layer of skin, the epidermis, is cornified epithelial tissue composed of keratinocytes. To maintain the structure and function of the epidermis, the regulation of proliferation, differentiation, and cornification of keratinocytes is crucial, and various soluble factors secreted by keratinocytes are involved in these regulations. Previously, work has shown that keratinocytes secreted the protein Kdap (keratinocyte differentiation-associated protein) associated with the formation of cornified cell envelopes, a specialized protective barrier structure on the periphery of terminally differentiating keratinocytes. In the present report, the canine counterpart of human Kdap is identified and an attempt has been made to define its physiological role in canine keratinization. Canine Kdap (cKdap) showed structural features commonly observed in other counterparts and is secreted from transfected cells. The expression profile of cKdap mRNA, which was restrictively expressed in cornified epithelial tissues besides skin has also been determined. These findings indicate that there is a strong association between cKdap expression and cornification, which supports previous observations that Kdap is involved in the synthesis and/or degradation of cornified cell envelopes in humans and mice.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yagihara
authors.forename: Y
authors.initials: Y
authors.lastname: Terada
authors.forename: S
authors.initials: S
authors.lastname: Sugimoto
authors.forename: F
authors.initials: F
authors.lastname: Hidaka
authors.forename: O
authors.initials: O
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Washizu
authors.forename: K
authors.initials: K
authors.lastname: Ariizumi
authors.forename: M
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D003001:Cloning, Molecular; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity
chemical_list: D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; C104073:Kdap protein, mouse