Isolation and characterization of Pseudomonas sp. strain HF-1, capable of degrading nicotine.
A nicotine-degrading bacterium, strain HF-1, was isolated from tobacco waste-contaminated soil and identified as a member of Pseudomonas sp. based on morphology, physiological tests, Vitek system (GN+), Biolog GN, 16S rDNA sequence and phylogenetic characteristics. The thermal denaturation test indicated that the G+C content of the DNA of HF-1 was 62.7 mol%. The relationship between growth and nicotine degradation of the isolate suggested that strain HF-1 could utilize nicotine as sole source of carbon, nitrogen and energy. Viridescent pigment was observed during nicotine degradation by strain HF-1. The isolate grew optimally at 30 degrees C, initial pH 6.5-7.5 and 1.3 g l(-1) of nicotine concentration in the nicotine inorganic salt medium, which is basically consistent with degradation of nicotine by the isolate. Strain HF-1 could degrade 99.6% of nicotine under the optimized incubation conditions for 25 h monitored by high-performance liquid chromatography. This study demonstrates that Pseudomonas sp. strain HF-1 had strong ability to degrade nicotine, and may be useful for bioremediation of environments contaminated by tobacco waste.
Ruan
Aidong
A
College of Life Sciences, Zhejiang University, Hangzhou 310029, PR China.
Min
Hang
H
Peng
Xiaohui
X
Huang
Zheng
Z
D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D018533:Biomass; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009538:Nicotine / Q000378:metabolism*; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011549:Pseudomonas / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D009538:Nicotine