title: Evaluation of (GTG)5-PCR for identification of Enterococcus spp.
abstract: A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG)(5) primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG)(5)-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG)(5) primer is a reliable and fast method for species identification of enterococci.
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Brno, Czech Republic. mpavel@sci.muni.cz
authors.forename: Pavel
authors.initials: P
authors.lastname: Svec
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.forename: Milan
authors.initials: M
authors.lastname: Seman
authors.forename: Cindy
authors.initials: C
authors.lastname: Snauwaert
authors.forename: Karen
authors.initials: K
authors.lastname: Lefebvre
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedlácek
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
mesh_terms: D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial; D016983:Enterococcus / Q000145:classification / Q000235:genetics*; D005516:Food Microbiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial