title: Hsp72 recognizes a P binding motif in the measles virus N protein C-terminus.
abstract: The major inducible 70-kDa heat shock protein (hsp72) binds measles virus (MV) nucleocapsids and increases MV gene expression. The cytoplasmic tail of the MV N protein (N(TAIL)) contains three hydrophobic domains (Box-1-3) that are potential targets of hsp72 interaction. Low affinity binding to Box-3 is correlated to hsp72-dependent stimulation of MV minireplicon reporter gene expression whereas interactions between hsp72 and Box-1 and/or -2 have not been documented. The present work showed that virus deficient in Box-3/hsp72 interaction retains the ability to form nucleocapsid/hsp72 complexes, identifying Box-2 but not Box-1 as a mediator of high affinity hsp72 binding. Box-2 is the binding site for the viral P protein X domain (XD), where P tethers the viral polymerase to nucleocapsid in support of transcription and genome replication, and competitive inhibition of XD binding to N(TAIL) by hsp72 was shown. Recognition of a common binding site by P and hsp72 represents a potential mechanism for host cell modulation of viral gene expression.
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, 43210, USA.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Bourhis
authors.forename: Sonia
authors.initials: S
authors.lastname: Longhi
authors.forename: Thomas
authors.initials: T
authors.lastname: Carsillo
authors.forename: Matthew
authors.initials: M
authors.lastname: Buccellato
authors.forename: Benjamin
authors.initials: B
authors.lastname: Morin
authors.forename: Bruno
authors.initials: B
authors.lastname: Canard
authors.forename: Michael
authors.initials: M
authors.lastname: Oglesbee
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D002460:Cell Line; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans / Q000378:metabolism; D008459:Measles virus / Q000737:chemistry*; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D014779:Virus Replication
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D019590:Nucleocapsid Proteins