title: Lactosucrose production by various microorganisms harboring levansucrase activity.
abstract: Production of the artificial sweetener, lactosucrose, by various microorganisms containing levansucrase activity was investigated. Of the tested bacteria, Bacillus subtilis was the most effective producer using lactose as an acceptor and sucrose as a fructosyl donor. Lactosucrose production by this strain was optimal at pH 6.0 and 55 degrees C whereupon 181 g lactosucrose l(-1) was produced from 225 g lactose l(-1) and 225 g sucrose l(-1) in 10 h.
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, 143-747, Korea.
authors.forename: Nyun-Ho
authors.initials: NH
authors.lastname: Park
authors.forename: Ha-Jong
authors.initials: HJ
authors.lastname: Choi
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism*; D004187:Disaccharides / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000031:analogs & derivatives / Q000378:metabolism*; D013395:Sucrose / Q000031:analogs & derivatives / Q000378:metabolism*; D013549:Sweetening Agents / Q000378:metabolism*; D013696:Temperature
chemical_list: D004187:Disaccharides; D013549:Sweetening Agents; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase; D007785:Lactose