Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture.
This paper reports the effects of exposure to static, sinusoidal (50 Hz), and combined static/sinusoidal magnetic fields on cultured astroglial cells. Confluent primary cultures of astroglial cells were exposed to a 1-mT sinusoidal, static, or combined magnetic field for 1h. In another experiment, cells were exposed to the combined magnetic field for 1, 2, and 4h. The hsp25, hsp60, hsp70, actin, and glial fibrillary acidic protein contents of the astroglial cells were determined by immunoblotting 24h after exposure. No significant differences were seen between control and exposed cells with respect to their contents of these proteins, neither were any changes in cell morphology observed. In a third experiment to determine the effect of a chronic (11-day) exposure to a combined 1-mT static/sinusoidal magnetic field on the proliferation of cultured astroglial cells, no significant differences were seen between control, sham-exposed, or exposed cells. These results suggest that exposure to 1-mT sinusoidal, static, or combined magnetic fields has no significant effects on the stress, cytoskeletal protein levels in, or proliferation of cultured astroglial cells.
Bodega
G
G
Departamento de Biología Celular y Genética, Facultad de Biología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. guillermo.bodega@uah.es
Forcada
I
I
Suárez
I
I
Fernández
B
B
D000199:Actins / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
D000199:Actins; D006360:Heat-Shock Proteins