Investigation of the accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) in rat kidneys.
The accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) and its metabolite 2,4-dichlorophenol (2,4-DCP) in the kidneys of rats was investigated. Male and female Sprague-Dawley rats were given 2,4-D in drinking water and food for 30 days. Group A (control group) was fed a normal diet, Group B was fed 50 ppm 2,4-D in 15 g food, Group C received 100 ppm 2,4-D in 15 g food, Group D received 25 ppm 2,4-D in 15 ml drinking water and Group E was given 50 ppm 2,4-D in 15 ml of drinking water. Levels of 2,4-D and 2,4-DCP in kidneys were determined using high performance liquid chromatography (HPLC). It was observed that at low doses of 2,4-D, the metabolite, 2,4-DCP found in the kidneys.
Aydin
Handan
H
University Istanbul, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, 34310 Avcilar, Istanbul, Turkey. haydin@istanbul.edu.tr
Ozdemir
Nurullah
N
Uzunören
Nuray
N
D015084:2,4-Dichlorophenoxyacetic Acid / Q000008:administration & dosage / Q000032:analysis*; D000821:Animal Feed; D000818:Animals; D000871:Anthelmintics / Q000032:analysis; D002733:Chlorophenols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D005554:Forensic Medicine; D006540:Herbicides / Q000008:administration & dosage / Q000032:analysis*; D007668:Kidney / Q000737:chemistry*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000737:chemistry
D000871:Anthelmintics; D002733:Chlorophenols; D006540:Herbicides; D014867:Water; D015084:2,4-Dichlorophenoxyacetic Acid; C004762:2,4-dichlorophenol