title: Use of proteomics to study chemosensitivity.
abstract: Chemoresistance remains an unresolved problem in clinical oncology. Therefore it is important to identify molecular factors that lead to an understanding of the mechanisms of drug resistance in cancer cells. On the protein-expression level, this can be done using proteomics, which has become the focus of significant interest and research over the past decade. We describe an easy and practicable standardized technique that can be used to study global protein expression in chemosensitive and chemoresistant cancer cells to find candidate proteins that are potentially associated with the drug-resistant phenotype. As an example, fractionation of human neuroblastoma cells using two-dimensional polyacrylamide electrophoresis, spot detection, image analysis, and finally protein identification is illustrated.
authors.affiliation: Institut für Laboratoriumsmedizin und Pathobiochemie, Universitätsklinikum Charite, Berlin, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Poland
authors.forename: Silke
authors.initials: S
authors.lastname: Wandschneider
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
authors.forename: Sergio
authors.initials: S
authors.lastname: Bernardini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Pranav
authors.initials: P
authors.lastname: Sinha
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D009363:Neoplasm Proteins; D009447:Neuroblastoma / Q000188:drug therapy*; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D020543:Proteome