Glyco-oxidation in diabetes and related diseases.
BACKGROUND
Recent evidence has shown that hyperglycemia is able to cause increased production of superoxide on the mitochondrial transport chain, and that this is the key event which activates some events such as increased AGE formation, increased hexosamine and polyol flux, and activation of PKC, all believed to be important for the development of the chronic complications of diabetes, aging and uremia. In this context, non-enzymatic protein glyco-oxidation leads to the formation of a series of products whose intra- and extra-cellular accumulation is of key importance in the pathogenesis of these chronic diseases.


METHODS
Various spectrometric approaches, such as matrix-assisted laser desorption ionisation (MALDI), and liquid chromatography-electrospray ionisation (ESI) were used.


CONCLUSIONS
The latest mass spectrometric approaches have shown their power in proteomics, and we report here some applications of this technique in the study of in vitro and in vivo non-enzymatic protein glyco-oxidation.
Lapolla
Annunziata
A
Dipartimento di Scienze Mediche e Chirurgiche-Cattedra di Malattie del Metabolismo, Università degli Studi di Padova, Via Giustiniani 2, 35100 Padova, Italy. annunziata.lapolla@unipd.it
Fedele
Domenico
D
Traldi
Pietro
P
D003920:Diabetes Mellitus / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D040901:Proteomics
D017127:Glycation End Products, Advanced; D005947:Glucose