[Effects of centrifuge training on mRNA expression in different rat tissues].
OBJECTIVE
To demonstrate the tissue specific expression of five rat genes related to centrifuge training and the relationship between expression change and duration of training.


METHODS
mRNA was extracted from hearts, brains, kidneys, lungs and intestines of rats after different durations of training. Five genes obtained by SSH from centrifuge-trained rats were labeled by Dig-11-dUTP as probes. The expression levels were measured by thin-line hybridization. Optical density (OD) values were obtained by scanning and treated with statistics.


RESULTS
As compared with control group, the new gene CH157 expression decreased in hearts and brains of rats trained for 6 d (P<0.05), but returned to control levels after 12 d training; the new gene CH244 expression depressed in rat hearts, brains, and intestines after 6 d or 12 d training, but it was not statistically significant owing to large individual differences; gene expression of protein inhibitor of neuronal nitric oxide syntheses (PIN) increased gradually and significantly in rat hearts (P<0.01 after 12 d training) and kidneys (P<0.05 after 12 d training); Cyt b gene expression in rat hearts and brains increased increasingly (P<0.01 after 12 d training), however, it increased obviously (P<0.05) in kidneys, lungs and intestines of rats trained for 6 d, while it returned back a little in rats trained for 12 d; enlongation factor 1-alpha expression levels in intestines of rats trained for 6 d increased significantly (P<0.05), but it became no significant in rats trained for 12 d, while the expression in brains increased gradually (P<0.01).


CONCLUSIONS
It suggested that the change in gene expression in several tissues of rats caused by centrifuge training contribute in enhancing +Gz tolerance.
Li
Li-ping
LP
Department of Biochemistry, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou, China. li@163.tom.com
Liu
Li
L
Yu
Li-shen
LS
Luo
Chao-quan
CQ
D000054:Acceleration; D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D001921:Brain; D002498:Centrifugation; D015870:Gene Expression / Q000502:physiology*; D006321:Heart; D018471:Hypergravity; D007422:Intestines; D007668:Kidney; D008168:Lung; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution
D012333:RNA, Messenger