title: Comparison of autologous cell therapy and granulocyte-colony stimulating factor (G-CSF) injection vs. G-CSF injection alone for the treatment of acute radiation syndrome in a non-human primate model.
abstract: OBJECTIVE
  To compare the efficacy of autologous cell therapy after irradiation combined with granulocyte-colony stimulating factor (G-CSF) injections with G-CSF treatment alone in a heterogeneous model of irradiation representative of an accidental situation.
  
  
  METHODS
  Non-human primates were irradiated at 8.7 Gy whole-body dose with the right arm shielded to receive 4.8 Gy. The first group of animals received G-CSF (lenograstim) injections starting 6 h after irradiation, and a second group received a combination of G-CSF (lenograstim) injections and autologous expanded hematopoietic cells. Animals were followed up for blood cell counts, circulating progenitors, and bone marrow cellularity.
  
  
  RESULTS
  No significant differences were seen between the two treatment groups, whatever the parameter observed: time to leukocyte or platelet recovery and duration and severity of aplasia.
  
  
  CONCLUSIONS
  Our results indicated that identical recovery kinetic was observed when irradiated animals are treated with G-CSF independently of the reinjection of ex vivo expanded autologous hematopoietic cells. Thus G-CSF injections might be chosen as a first-line therapeutic strategy in the treatment of accidental acute radiation victims.
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire, Service de Radiobiologie et d'Epidémiologie, Laboratoire de Thérapie Cellulaire et de Radioprotection Accidentelle, Fontenay-aux-Roses, France. jean-marc.bertho@irsn.fr
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Bertho
authors.forename: Johanna
authors.initials: J
authors.lastname: Frick
authors.forename: Marie
authors.initials: M
authors.lastname: Prat
authors.forename: Christelle
authors.initials: C
authors.lastname: Demarquay
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dudoignon
authors.forename: François
authors.initials: F
authors.lastname: Trompier
authors.forename: Norbert-Claude
authors.initials: NC
authors.lastname: Gorin
authors.forename: Dominique
authors.initials: D
authors.lastname: Thierry
authors.forename: Patrick
authors.initials: P
authors.lastname: Gourmelon
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006410:Hematopoiesis / Q000528:radiation effects*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D000078224:Lenograstim; D008252:Macaca fascicularis; D008297:Male; D011833:Radiation Injuries, Experimental / Q000628:therapy*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013577:Syndrome; D016896:Treatment Outcome
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000078224:Lenograstim