[Effect of femoral vein-occlusion on replanted limb survival at different stages].
OBJECTIVE
To study the effect of vein-occlusion on the replanted limb survival in SD rats at different stages.


METHODS
Twenty-five adult SD rats were randomly divided into 5 groups according to the time of the femoral vein occlusion after the replanted limbs: 2-, 3-, 4-, 6-, and 8-day groups. The limbs were observed through naked eye, measurement of dermal temperature and angiography.


RESULTS
No formation of collateral veinlet was found, and necrosis was seen in the replanted limbs of 2-, 3-day groups. Reflux-vein was gradually increased in the replanted limbs of 4, 6, and 8 day groups. Angiographic score of capillary density and dermal temperature in the thigh muscles were greater in groups 4-, 6-, and 8-day than in groups 2- and 3-day.


CONCLUSIONS
Within 2 and 3 days,the replanted limbs of SD rats will necrose because of vein-occlusion; and 4 days later the replanted limbs can survive depending on the reflux-vein of new collateral veinlet.
Yuan
Feng
F
Department of Orthopedics, Tongji Hospital Affiliated to Tongji University, Shanghai, 200065, PR China.
Yu
Guangrong
G
Cai
Xuansong
X
D000818:Animals; D005260:Female; D005268:Femoral Vein / Q000601:surgery*; D006614:Hindlimb / Q000098:blood supply* / Q000293:injuries / Q000601:surgery; D008026:Ligation / Q000009:adverse effects; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012092:Replantation / Q000379:methods; D013997:Time Factors; D014022:Tissue Survival; D014652:Vascular Diseases / Q000209:etiology / Q000503:physiopathology*