DNA microarray analysis of the nitrogen starvation response of Corynebacterium glutamicum.
Nitrogen is an essential component of nearly all of the complex macromolecules in a bacterial cell, e.g. proteins, nucleic acids, and cell wall components. Accordingly, most prokaryotes have developed elaborate control mechanisms to provide an optimal supply of nitrogen for cellular metabolism and to cope with situations of nitrogen limitation. In this communication, a global analysis of the Corynebacterium glutamicum nitrogen starvation response by transcriptional profiling using DNA microarrays is presented. Our results show that C. glutamicum reacts to nitrogen starvation with a rearrangement of the cellular transport capacity, changes in metabolic pathways concerning nitrogen assimilation and amino acid biosynthesis, and a decreased capacity for protein synthesis.
Silberbach
Maike
M
Institut für Biochemie, Universität zu Köln, Zülpicher Strasse 47, D-50674 Köln, Germany.
Hüser
Andrea
A
Kalinowski
Jörn
J
Pühler
Alfred
A
Walter
Britta
B
Krämer
Reinhard
R
Burkovski
Andreas
A
D000222:Adaptation, Physiological / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism*
D001426:Bacterial Proteins; D020543:Proteome; D014157:Transcription Factors; D009584:Nitrogen