title: The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.
abstract: Centromeres of fission yeast are arranged with a central core DNA sequence flanked by repeated sequences. The centromere-associated histone H3 variant Cnp1 (SpCENP-A) binds exclusively to central core DNA, while the heterochromatin proteins and cohesins bind the surrounding outer repeats. CHD (chromo-helicase/ATPase DNA binding) chromatin remodeling factors were recently shown to affect chromatin assembly in vitro. Here, we report that the CHD protein Hrp1 plays a key role at fission yeast centromeres. The hrp1Delta mutant disrupts silencing of the outer repeats and central core regions of the centromere and displays chromosome segregation defects characteristic for dysfunction of both regions. Importantly, Hrp1 is required to maintain high levels of Cnp1 and low levels of histone H3 and H4 acetylation at the central core region. Hrp1 interacts directly with the centromere in early S-phase when centromeres are replicated, suggesting that Hrp1 plays a direct role in chromatin assembly during DNA replication.
authors.affiliation: Karolinska Institute, Department of Biosciences/Department of Natural Sciences, University College Sodertorn Alfred Nobel's Alle 7, S-141 89, Huddinge, Sweden.
authors.forename: Julian
authors.initials: J
authors.lastname: Walfridsson
authors.forename: Pernilla
authors.initials: P
authors.lastname: Bjerling
authors.forename: Maria
authors.initials: M
authors.lastname: Thalen
authors.forename: Eung-Jae
authors.initials: EJ
authors.lastname: Yoo
authors.forename: Sang Dai
authors.initials: SD
authors.lastname: Park
authors.forename: Karl
authors.initials: K
authors.lastname: Ekwall
mesh_terms: D000107:Acetylation; D000251:Adenosine Triphosphatases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle; D002503:Centromere / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D020090:Chromosome Segregation; D004265:DNA Helicases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C410349:Cnp1 protein, S pombe; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; D000251:Adenosine Triphosphatases; C111321:hrp1 protein, S pombe; D004265:DNA Helicases