[Study on inhibition on alpha-glucosidase and analysis of the hypoglycemic composition of lobster sauce].
OBJECTIVE
To study the effects of lobster sauce and polysaccharide extracts on alpha-1,4-glucosidase activity by establishing on enzyme-inhibitor model.


METHODS
Glucosidase activity was assayed at PH optima by PNPG substrate. Lobster sauce extracts were prepared by soaking, centrifagation. Polysaccharide were prepared by soaking, centrifagation, separation.


RESULTS
Lobster sauce and polysaccharide can inhibit alpha-1,4-glucosidase.


CONCLUSIONS
This finding suggested that their hypoglycemic mechanism was similar to Acarbose. Lobster sauce may contain phenyalanine.
Guo
Ruihua
R
North China Coal Medical College, Tangshan 063000.
Zhai
Li
L
Liu
Zhengmeng
Z
Wang
Heping
H
Zhao
Yunsen
Y
Sun
Yanmei
Y
Zou
Hongbo
H
D001786:Blood Glucose / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004789:Enzyme Activation; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D011134:Polysaccharides; D014867:Water