Differential expression and distribution of alternatively spliced transcripts of PDGF-A and of PDGF receptor-alpha in mouse reproductive tissues.
Platelet-derived growth factor-A (PDGF-A) affects cellular activities such as proliferation, differentiation, and development by way of paracrine or autocrine interaction with PDGF-A receptor alpha (PDGFR-alpha). Two forms of alternatively spliced PDGF-A mRNA, a long and a short isoform, have been found in several mammalian species. Expression of PDGF-A and its cognate receptor PDGFR-alpha has been well studied in various tissues. However, these investigations did not distinguish between the individual isoforms of PDGF-A. In the present investigation, we identified the differential cellular expression patterns of the two isoforms of PDGF-A and of PDGFR-alpha in mouse reproductive tissues by using laser capture microdissection coupled with reverse transcriptase polymerase chain reaction. The long PDGF-A mRNA isoform was primarily detected in the epithelium, while the short isoform was ubiquitously distributed in epithelium, stroma, and muscle cells, although it was still more prominent in epithelium. PDGFR-alpha was mainly detected in stromal and muscle cells. Also, it was found in the epididymal epithelium, mucosal folds of the seminal vesicle, and ovarian granulosa cells. Thus, the complete PDGF-A/PDGFR-alpha signaling system is present in murine reproductive tissues, but the distribution of the long and short isoforms of PDFG-A differs.
Li
Sheng-Hsiang
SH
Division of Reproduction and Endocrinology, Department of Medical Research, Mackay Memorial Hospital, Tamshui 25115, Taipei, Taiwan, ROC.
Lee
Robert Kuo-Kuang
RK
Chen
Peng-Wu
PW
Lu
Chung-Hao
CH
Wang
Shu-Huei
SH
Hwu
Yuh-Ming
YM
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003850:Deoxyribonuclease I / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D005835:Genitalia / Q000378:metabolism*; D005836:Genitalia, Female / Q000378:metabolism; D005837:Genitalia, Male / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
D018076:DNA, Complementary; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C075278:platelet-derived growth factor A; D020796:Receptor, Platelet-Derived Growth Factor alpha; D003850:Deoxyribonuclease I