title: African origin of GB virus C determined by phylogenetic analysis of a complete genotype 5 genome from South Africa.
abstract: GB virus C (GBV-C), a positive-strand RNA virus, currently infects approximately one-sixth of the world's population. This virus exists as a collection of genotypes whose global distribution correlates with geographical origin. Genotyping of GBV-C isolates by phylogenetic analysis has relied upon the use of 5'-untranslated region (5'-UTR) sequences, however, complete genome sequences are used to demonstrate definitively their existence and geographical correlation. Initial identification of the fifth genotype from South Africa was based upon phylogenetic analysis of the 5'-UTR. It was sought to confirm this classification by analysis of full-length E2 genes from South African isolates and by analysis of a complete genotype 5 genome. Analysis of full-length E2 genes from 28 GBV-C-infected South African individuals revealed the existence of a unique group of 18 isolates, distinct from the other four genotypes. Bootstrap analysis provided strong support (95 %) for this fifth group. The remaining isolates were either genotype 1 (n=8) or 2 (n=2). Analysis of human E2 gene sequences, with the E2 gene from the chimpanzee variant GBV-Ctro included as the outgroup, produced a tree rooted on the genotype 1 branch. The complete genome nucleotide sequence of South African genotype 5 isolate D50 was determined. Phylogenetic analysis of the 5'-UTR and open reading frame produced congruent trees that grouped the sequences into five major genotypes. Inclusion of the corresponding region of the chimpanzee isolate GBV-Ctro in the analysis produced trees rooted on the branch leading to the genotype 5 isolate D50, suggesting an ancient African origin of GBV-C.
authors.affiliation: Infectious Diseases Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Dept 9NB, Bldg AP20-4, 100 Abbott Park Road, Abbott Park, IL 60064-6015, USA.
authors.forename: A Scott
authors.initials: AS
authors.lastname: Muerhoff
authors.affiliation: Infectious Diseases Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Dept 9NB, Bldg AP20-4, 100 Abbott Park Road, Abbott Park, IL 60064-6015, USA.
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Leary
authors.affiliation: Infectious Disease Unit, Nelson R. Mandela School of Medicine, Doris Duke Research Institute, University of KwaZulu-Natal, South Africa.
authors.forename: Mahomed A
authors.initials: MA
authors.lastname: Sathar
authors.affiliation: Infectious Diseases Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Dept 9NB, Bldg AP20-4, 100 Abbott Park Road, Abbott Park, IL 60064-6015, USA.
authors.forename: George J
authors.initials: GJ
authors.lastname: Dawson
authors.affiliation: Infectious Diseases Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Dept 9NB, Bldg AP20-4, 100 Abbott Park Road, Abbott Park, IL 60064-6015, USA.
authors.forename: Suresh M
authors.initials: SM
authors.lastname: Desai
mesh_terms: D018178:Flaviviridae Infections / Q000821:virology; D029407:GB virus C / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D013019:South Africa; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins