title: [The selection and properties of Penicillium verruculosum mutants with enhanced production of cellulases and xylanases].
abstract: The paper describes three Penicillium verruculosum 28K mutants with about threefold enhanced production of five industrially important carbohydrases. The two-stage fermentation process that we developed provided a further two- to threefold increase in the production of carbohydrases. Physiological and biochemical studies showed that the synthesis of all five carbohydrases is inducible. Carboxymethylcellulase, xylanase, and beta-glucanase are synthesized under a common regulatory control, as is evident from the concurrent increase in the synthesis of these enzymes in the presence of microcrystalline cellulose. The synthesis of avicelase and beta-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
authors.forename: I V
authors.initials: IV
authors.lastname: Solov'eva
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: V V
authors.initials: VV
authors.lastname: Vel'kov
authors.forename: A V
authors.initials: AV
authors.lastname: Koshelev
authors.forename: T V
authors.initials: TV
authors.lastname: Bubnova
authors.forename: E G
authors.initials: EG
authors.lastname: Kondrat'eva
authors.forename: A A
authors.initials: AA
authors.lastname: Skomarovskiĭ
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism; D009154:Mutation; D010407:Penicillium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012641:Selection, Genetic; D014995:Xylosidases / Q000096:biosynthesis / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D003470:Culture Media; D002482:Cellulose; D006026:Glycoside Hydrolases; D014995:Xylosidases; C019822:carbohydrase; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase