In vivo optical imaging of human adenoid cystic carcinoma cell metastasis.
A noninvasive, whole-body, real-time fluorescence optical imaging of stable high-level green fluorescent protein (GFP)-expressing human adenoid cystic carcinoma (ACC-M-GFP) was demonstrated for in vivo visualization of metastatic behavior in nude mice. Five-week-old female nude mice were injected with ACC-M-GFP in the primary organ: submandibular gland. Metastases were only visualized by GFP expression in the lung. However, metastatic lesions of ACC-M-GFP in the lung, muscle, bladder and bony were found by imaging of GFP expression in intact mice through tail vein injection of ACC-M-GFP cells. The construction of highly fluorescent and stable GFP transfectants of ACC-M has revealed the multi-organ metastatic capability of ACC-M cells through this optical imaging.
Xiong
Tao
T
The Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China. xiongtao2005@yahoo.com.cn
Zhang
Zhihong
Z
Liu
Bi-Feng
BF
Zeng
Shaoqun
S
Chen
Yanping
Y
Chu
Jun
J
Luo
Qingming
Q
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000556:secondary*; D002294:Carcinoma, Squamous Cell / Q000556:secondary*; D049109:Cell Proliferation; D005260:Female; D049452:Green Fluorescent Proteins; D049408:Luminescent Agents; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation
D049408:Luminescent Agents; D049452:Green Fluorescent Proteins