Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco.
Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1-C11) were isolated and fused with a beta-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4-C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.
Shirasawa-Seo
Naomi
N
Miyagi Prefectural Agriculture and Horticulture Research Center, Takadate-kawakami, Natori, Miyagi 981-1243, Japan.
Sano
Yoshitaka
Y
Laboratory of Plant Pathology, Faculty of Agriculture, Niigata University, Ikarashi, Niigata 950-2181, Japan.|Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Nakamura
Shigeo
S
Miyagi Prefectural Agriculture and Horticulture Research Center, Takadate-kawakami, Natori, Miyagi 981-1243, Japan.
Murakami
Taka
T
National Institute of Agro-Biological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Seo
Shigemi
S
National Institute of Agro-Biological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Ohashi
Yuko
Y
National Institute of Agro-Biological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Hashimoto
Yoshifumi
Y
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Matsumoto
Tsuguo
T
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
D036022:Capsid Proteins / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D018519:Meristem / Q000378:metabolism; D025682:Nanovirus / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014026:Nicotiana; D014764:Viral Proteins / Q000378:metabolism
D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D014764:Viral Proteins