title: Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications.
abstract: We present a method for performing polymerase chain reaction (PCR) using isolated droplets flowing in an immiscible fluorinated solvent system. Thanks to an optimized control of interfacial properties, we could achieve in this capillary-based system reproducible amplification factors, without any detectable contamination between neighboring droplets. The system is readily amenable to further miniaturization and automation and serves as the first step toward a clinically viable, high-throughput, quantitative continuous flow PCR apparatus.
authors.affiliation: Laboratoire Physicochimie-Curie, UMR/CNRS 168, Institut Curie Section de Recherche, 26 Rue d'Ulm, F-75248 Paris Cedex, France.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Dorfman
authors.forename: Max
authors.initials: M
authors.lastname: Chabert
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Codarbox
authors.forename: Gilles
authors.initials: G
authors.lastname: Rousseau
authors.forename: Patricia
authors.initials: P
authors.lastname: de Cremoux
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
mesh_terms: D004247:DNA / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D004247:DNA