p53 autoantibodies from patients with head and neck cancer recognise common epitopes on the polypeptide chain of p53.
In the present study, we analysed p53 autoantibodies from patients with head and neck cancer by ELISA, by Western blot using C- and N-terminal fragments of p53 and with peptide libraries of p53. We found that 8.2% of the patients with head and neck cancer developed antibodies against p53. Using additional p53 autoantibody-positive sera from patients with head and neck cancer, we found that all of these sera recognised common epitopes, which were also recognised by well-known mouse monoclonal antibodies against p53. Common epitopes are located in regions on the polypeptide chain of p53, which are functionally important for the role of p53 in growth control. Thus, the knowledge of these epitopes might be useful for the development of new strategies for cancer therapy.
Saleh
Jumana
J
Medical Biochemistry and Molecular Biology, University of the Saarland, Building 44, 66424 Homburg, Germany.
Brunner
Christian
C
Gölzer
Ramona
R
Nastainczyk
Wolfgang
W
Montenarh
Mathias
M
D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000276:immunology*
D001323:Autoantibodies; D010446:Peptide Fragments; D016159:Tumor Suppressor Protein p53