[The study of salivary-SIgA reaction to Streptococcus mutans in acid environment].
OBJECTIVE
To test the salivary immunoglobulin A antibody activity to Streptococcus mutans in normal with in acid environment.


METHODS
Streptococcus mutans strains were isolated from 20 volunteers, serotyped by biochemical test and PCR, and genotyped by AP-PCR. Unstimulated secretions from submandibular glands and sublingual glands were collected from volunteers by modified collectors. Each identified Streptococcus mutans genotype was cultured in two groups: control group was cultured in BHI broth pH7.2 at 37 degrees C for 2 h; acid shock group were cultured in TYEG broth (pH5.5) at 37 degrees C for 2 h. Analysis of SIgA activity to Streptococcus mutans genotypes in different groups was detected by Western blot.


RESULTS
(1) The SIgA of each individual could response to his own Streptococcus mutans strains and the reference strains; (2) The same individual had different SIgA activity to different genotype strains; (3) There were no significant difference between acid groups and control groups, in spite that some bands had strong or weak intensity.


CONCLUSIONS
Although Streptococcus mutans could express acid shock proteins in stress, the present study suggests that these new proteins have no qualitative effect on the reaction of SIgA to Streptococcus mutans.
Nie
Min
M
Department of Operative Dentistry and Endodontics, School of Stomatology, Wuhan University, Wuhan 430079, China.
Fan
Hua-li
HL
Fan
Ming-wen
MW
Hu
Ping
P
Liu
Jia-rong
JR
Bian
Zhuan
Z
D000328:Adult; D003773:Dental Plaque / Q000276:immunology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology* / Q000378:metabolism
D007071:Immunoglobulin A, Secretory