title: ExsE, a secreted regulator of type III secretion genes in Pseudomonas aeruginosa.
abstract: Type III secretion systems are toxin delivery systems that are present in a large number of pathogens. A hallmark of all type III secretion systems studied to date is that expression of one or more of their components is induced upon cell contact. It has been proposed that this induction is controlled by a negative regulator that is itself secreted by means of the type III secretion machinery. Although candidate proteins for this negative regulator have been proposed in a number of systems, for the most part, a direct demonstration of their role in regulation is lacking. Here, we report the discovery of ExsE, a negative regulator of type III secretion gene expression in Pseudomonas aeruginosa. Deletion of exsE deregulates expression of the type III secretion genes. We provide evidence that ExsE is itself secreted by means of the type III secretion machinery and physically interacts with ExsC, a positive regulator of the type III secretion regulon. Taken together, these data demonstrate that ExsE is the secreted negative regulator that couples triggering of the type III secretion machinery to induction of the type III secretion genes.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, and Division of Infectious Diseases, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Arne
authors.initials: A
authors.lastname: Rietsch
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vallet-Gely
authors.forename: Simon L
authors.initials: SL
authors.lastname: Dove
authors.forename: John J
authors.initials: JJ
authors.lastname: Mekalanos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D040461:Gene Components; D015964:Gene Expression Regulation, Bacterial; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D037521:Virulence Factors / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D001616:beta-Galactosidase; D002118:Calcium