Caffeic acid inhibits oxidative Stress and reduces hypercholesterolemia induced by iron overload in rats.
The effects of caffeic acid, a major phenolic compound of the diet, on oxidative stress and cholesterolemia are studied in rats submitted to oxidative stress by iron overload. Male Wistar rats were fed semi-synthetic diets containing regular (50 mg/kg diet) or high (2000 mg/kg) doses of iron with and without caffeic acid (6460 mg/kg) for 4 weeks. The high doses of iron induced an increase of lipid oxidation in the liver, as measured by thiobarbituric acid-reactive substances (TBARS), and an increase of cholesterolemia. Caffeic acid fully prevented the pro-oxidant effects of high iron doses (p < 0.001). It also reduced lipid peroxidation in rats fed the low iron dose (p < 0.05). Caffeic acid also increased vitamin E levels in plasma (2.74 micromol/L to 4.09 micromol/L for normal diet; p < 0.001; 2.78 micromol/L to 4.94 micromol/L for iron supplemented diet p < 0.001). Iron-induced hypercholesterolemia was inhibited by caffeic acid (1.07 g/L to 0.82 g/L; p < 0.001). These results demonstrate the antioxidative capacity of caffeic acid, a highly bioavailable polyphenol, in an in vivo model of oxidative stress.
Lafay
Sophie
S
Unité des Maladies Métaboliques and Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 St-Genes-Champanelle, France.
Gueux
Elyett
E
Rayssiguier
Yves
Y
Mazur
André
A
Rémésy
Christian
C
Scalbert
Augustin
A
D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology* / Q000627:therapeutic use / Q000652:urine; D002784:Cholesterol / Q000097:blood; D003373:Coumaric Acids / Q000652:urine; D006937:Hypercholesterolemia / Q000139:chemically induced / Q000188:drug therapy*; D007501:Iron / Q000008:administration & dosage / Q000097:blood; D019190:Iron Overload / Q000150:complications*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000097:blood
D002109:Caffeic Acids; D003373:Coumaric Acids; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D014810:Vitamin E; D002784:Cholesterol; C004999:ferulic acid; D007501:Iron; C040048:caffeic acid