p53 immunohistochemical expression in Barrett's esophagus before and after endoscopic ablation by argon plasma coagulation.
OBJECTIVE
Few studies have evaluated p53 accumulation in the squamous mucosa contiguous (SMC) to Barrett's esophagus (BE) and in the new squamous epithelium after endoscopic ablation. We evaluated the p53 expression in BE, in the SMC, and in the new squamous mucosa generated after ablation by argon plasma coagulation (APC).


METHODS
Endoscopic biopsy specimens from 37 BE patients, before and after ablation by APC, were analyzed. The p53 immunostaining criterion used was the staining of at least half of the nuclei.


RESULTS
p53 was detected in BE in 5 (13.5%) cases. In all these cases, SMC was p53(+). In addition, SMC was p53(-) in all cases of p53(-) BE (p <0.001). In the 5 cases with p53(+) BE and SMC, the new squamous mucosa continued to be p53(+). However, in the 32 cases with p53(-) SMC, the new squamous mucosa was also p53(-) (p <0.001). No case with p53(+) SMC turned out to be p53(-) after ablation. Similarly, no case with p53(-) BE and SMC before eradication became p53(+) after ablation (p < 0.001).


CONCLUSIONS
p53 was highly prevalent in the contiguous squamous mucosa when it is present in BE. After ablation, none of the cases lost p53 expression, and none of the negative cases turned out to be positive.
Lopes
César Vivian
CV
Department of Pathology and Gastroenterology of the Porto Alegre School of Medical Sciences/Santa Casa Hospital, Porto Alegre, Brazil. cevele@terra.com.br
Pereira-Lima
Júlio
J
Hartmann
Antônio Atalíbio
AA
D000911:Antibodies, Monoclonal; D001471:Barrett Esophagus / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D015415:Biomarkers; D001706:Biopsy; D018450:Disease Progression; D004945:Esophagoscopy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D017075:Laser Coagulation / Q000379:methods*; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D011184:Postoperative Period; D011300:Preoperative Care; D015203:Reproducibility of Results; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016159:Tumor Suppressor Protein p53