title: Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis.
abstract: RNA interference (RNAi), a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), can be used not only as a research tool but also as a therapeutic strategy for viral infection. We demonstrated that intracellular expression of short hairpin RNA (shRNA) targeting human cyclin T1 (hCycT1), a cellular factor essential for transcription of messenger and genomic RNAs from the long terminal repeat promoter of provirus of human immunodeficiency virus type 1 (HIV-1), could effectively suppress the replication of HIV-1. We also showed that downregulation of hCycT1 did not cause apoptotic cell death, therefore, targeting cellular factor hCycT1 by shRNAs may provide an attractive approach for genetic therapy of HIV-1 infection in the future.
authors.affiliation: The Modern Virology Research Centre and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yu
authors.initials: Y
authors.lastname: Peng
authors.forename: Ji'an
authors.initials: J
authors.lastname: Pan
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wu
authors.forename: Snawar
authors.initials: S
authors.lastname: Hussain
authors.forename: Po
authors.initials: P
authors.lastname: Tien
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D056906:Cyclin T; D016213:Cyclins / Q000172:deficiency* / Q000235:genetics*; D015536:Down-Regulation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014779:Virus Replication
chemical_list: C503684:CCNT1 protein, human; D056906:Cyclin T; D016213:Cyclins; D012313:RNA