Anti-inflammatory activity of Korean folk medicine 'Sabaeksan'.
Sabaeksan has been used for prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of Sabaeksan, we examined the effect of Sabaeksan on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced pro-inflammatory cytokines secretion in human mast cell line HMC-1 cells. Sabaeksan by itself had no effect on viability of HMC-1 cells. The effects of Sabaeksan on the secretion of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Sabaeksan (1 mg/ml) inhibited PMA plus A23187-induced TNF-alpha, IL-6, and IL-8 secretion by 43.86+/-5.26%, 56.39+/-3.65%, and 63.48+/-2.54%, respectively. Sabaeksan also inhibited the nuclear factor-kappa B (NF-kappaB) activation and IkappaBalpha degradation. Taken together, these results suggest that Sabaeksan inhibits the secretion of pro-inflammatory cytokines in HMC-1 cells through blockade of NF-kappaB activation.
Choi
In-Young
IY
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South Korea.
Koo
Hyun-Na
HN
Lee
Si-Hyeong
SH
Shin
Jo-Young
JY
Chae
Han-Jung
HJ
Kim
Hyung-Ryong
HR
Kim
Cheorl-Ho
CH
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D007723:Korea; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008518:Medicine, East Asian Traditional; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D002273:Carcinogens; D016207:Cytokines; D007476:Ionophores; D016328:NF-kappa B; D010936:Plant Extracts; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate