title: Typing class I HLA-A gene using a nested PCR-RFLP procedure.
abstract: In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
authors.affiliation: TOXICAN, Departamento de Patologia, Faculdade de Medicina, Universidade Estadual Paulista, 186180-000 Botucatu, SP, Brasil. erickcastelli@terra.com.br
authors.forename: E C
authors.initials: EC
authors.lastname: Castelli
authors.forename: D S
authors.initials: DS
authors.lastname: Gil
authors.forename: L C S
authors.initials: LC
authors.lastname: Veiga
authors.forename: J L V
authors.initials: JL
authors.lastname: de Camargo
mesh_terms: D000483:Alleles; D005805:Genes, MHC Class I / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D015234:HLA-A Antigens