title: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation.
abstract: Using two-colour flow cytometry >200 antibodies submitted to the 8th International Workshop of Human Leukocyte Differentiation Antigens (HLDA8) have been analyzed for their reactivity with resting and activated CD203c+ basophils. Four antibodies either non-reactive or weakly reactive with resting basophils exhibited an increased reactivity with basophils activated by anti-IgE-mediated cross-linking of the high affinity IgE receptor (FceRI). These include antibodies against CD164 (WS-80160, clone N6B6 and WS-80162, clone 67D2), as well as two reagents with previously unknown specificities that were identified as CD13 (WS-80274, clone A8) and CD107a (WS-80280, clone E63-880). The activation patterns followed either the "CD203c-like" or "CD63-like" activation profile. The CD203c profile is characterized by a rapid and significant upregulation (of CD13, CD164, and CD203c), reaching maximum levels after 5-15 min of stimulation. The Phosphoinositide-3-kinase (PI3K)-specific inhibitor Wortmannin inhibited the upregulation of these markers whereas 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induced a rapid and FceRI-independent upregulation within 1-2 min. In the CD63 profile, maximum upregulation (of CD63 and CD107a) was detected only after 20-40 min, and upregulation by TPA reached maximum levels after 60 min. In summary, our data identify CD13, CD107a, and CD164 as novel basophil-activation antigens. Based on time kinetics of upregulation, we hypothesize that molecules of the "CD203c group" and the "CD63 group" are linked to two different mechanisms of basophil activation.
authors.affiliation: University Clinic of Tübingen, Department of Internal Medicine II, Division of Hematology and Immunology, Tübingen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Hennersdorf
authors.forename: Stefan
authors.initials: S
authors.lastname: Florian
authors.forename: Andreas
authors.initials: A
authors.lastname: Jakob
authors.forename: Katharina
authors.initials: K
authors.lastname: Baumgärtner
authors.forename: Karoline
authors.initials: K
authors.lastname: Sonneck
authors.forename: Alfred
authors.initials: A
authors.lastname: Nordheim
authors.forename: Tilo
authors.initials: T
authors.lastname: Biedermann
authors.forename: Peter
authors.initials: P
authors.lastname: Valent
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Bühring
mesh_terms: D000730:Androstadienes / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001491:Basophils / Q000166:cytology / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism*; D018826:CD13 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D051930:Endolyn / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000276:immunology; D052118:Lysosomal-Associated Membrane Protein 1 / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000276:immunology; D010727:Phosphoric Diester Hydrolases / Q000276:immunology; D015230:Prostaglandin D2 / Q000276:immunology; D011755:Pyrophosphatases / Q000276:immunology; D017455:Receptors, IgE / Q000276:immunology; D015854:Up-Regulation; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D000906:Antibodies; D015415:Biomarkers; C497898:CD164 protein, human; C427944:ENPP3 protein, human; D051930:Endolyn; D000939:Epitopes; D007166:Immunosuppressive Agents; D052118:Lysosomal-Associated Membrane Protein 1; D017455:Receptors, IgE; D007073:Immunoglobulin E; D010727:Phosphoric Diester Hydrolases; D018826:CD13 Antigens; D011755:Pyrophosphatases; D015230:Prostaglandin D2; D000077191:Wortmannin