In vivo follow-up of rat tumor models with 2-deoxy-2-[F-18]fluoro-D-glucose/dual-head coincidence gamma camera imaging.
Before studying the impact of 2-deoxy-2-[F-18]fluoro-D-glucose (FDG) imaging with a dual-head coincidence gamma camera (DHC) for the follow-up of animal tumor models, we wanted to optimize this technique.


METHODS
Three different animal tumor models (osteosarcoma, melanoma, and breast cancer) were studied after FDG injection. Dynamic and dual time point FDG/DHC imaging were studied from one hour to five hours postinjection. In vitro tumor cell FDG uptake was assessed in eight different tumor cell lines. In one model (osteosarcoma), tumor growth, lung metastasis emergence, and survival were assessed by classical clinical follow-up and compared to FDG imaging in a control group (n = 6) and in a group treated by endostatin liposome complexes (n = 6).


RESULTS
Images obtained five hours after injection were more reliable for tumor growth follow-up than standard images (one hour). In vitro tumor cell FDG uptake confirmed in vivo imaging studies. In eight different tumor cell lines the FDG uptake was higher after five hours incubation than after one hour (p < 0.002). With FDG follow-up, we found that FDG uptake was strongly correlated with survival and that lung metastasis larger than 5 mm could be detected.


CONCLUSIONS
Using the optimization proposed above, DHC/FDG functional imaging seems to be a powerful tool to study rat tumor models and to help develop novel cancer therapies.
Monteil
Jacques
J
Service de Médecine Nucléaire, CHU Dupuytren, 2 Avenue Martin Luther-King, 87042, Limoges Cedex, France. jacques.monteil@unilim.fr
Dutour
Aurélie
A
Akla
Barbara
B
Chianéa
Thierry
T
Le Brun
Valérie
V
Grossin
Laurent
L
Paraf
François
F
Petegnief
Yolande
Y
Vandroux
Jean-Claude
JC
Rigaud
Michel
M
Sturtz
Franck G
FG
D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000494:pharmacology; D005500:Follow-Up Studies; D015902:Gamma Cameras; D006801:Humans; D009362:Neoplasm Metastasis / Q000473:pathology; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D013997:Time Factors
D019788:Fluorodeoxyglucose F18