title: A transcriptome map of cellular transformation by the fos oncogene.
abstract: BACKGROUND
  The c-fos gene was originally identified as the cellular homolog of the oncogene v-fos carried by the Finkel-Biskis-Jenkins and Finkel-Biskis-Reilly murine osteogenic sarcoma retroviruses. Sustained expression of fos is sufficient to induce cellular transformation in vitro and tumorigenesis in vivo. Fos functions as a component of the AP-1 transcription factor complex to regulate gene transcription and several differentially expressed genes have been identified in cells transformed by fos. We have extended these studies by constructing a cellular system for conditional transformation by v-fos. Using Affymetrix-based DNA microarray technology, we analyzed transcriptional changes over the course of transformation and reversion in an inducible v-fos system.
  
  
  RESULTS
  Microarray analyses of temporal gene expression during the process of v-fos mediated cellular transformation and morphological reversion revealed a remarkably dynamic transcriptome. Of the more than 8000 genes analyzed in this study, 3766 genes were categorized into 18 gene-expression patterns by using self-organizing map analysis. By combining the analysis of gene expression profiles in stably transformed cells with the analysis of sequential expression patterns during conditional transformation, we identified a relatively small cohort of genes implicated in v-fos mediated cellular transformation.
  
  
  CONCLUSIONS
  This approach defines a general conditional cell transformation system that can be used to study the endogenous transcription regulatory mechanisms involved in transformation and tumorigenesis. In addition, this study is the first reported analysis of dynamic changes in gene expression throughout experimentally controlled morphological transformation mediated by v-fos.
authors.affiliation: Orion Genomics, 4041 Forest Park Ave., St. Louis, MO 63108, USA. jordway@oriongenomics.com
authors.forename: Jared M
authors.initials: JM
authors.lastname: Ordway
authors.forename: Steven D
authors.initials: SD
authors.lastname: Fenster
authors.forename: Hong
authors.initials: H
authors.lastname: Ruan
authors.forename: Thomas
authors.initials: T
authors.lastname: Curran
mesh_terms: D002460:Cell Line; D048430:Cell Shape; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D007763:Lac Operon / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016761:Oncogene Proteins v-fos / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D016761:Oncogene Proteins v-fos