Induction of active immune suppression by co-immunization with DNA- and protein-based vaccines.
Although immunization has been used for eliciting immune response, here we show that it can also induce immune suppression. When a DNA vaccine encoding a viral antigen such as the VP1 protein from the foot and mouth disease virus is administered together with its recombinant protein antigen or a viral preparation containing the same antigen, the immunized animals developed significantly reduced antigen-specific T cell-mediated responses and became impaired to subsequent rechallenge with the same antigen. The induction of immune suppression is mediated by suppressor T cells, as demonstrated by an adoptive transfer experiment and mixed lymphocyte reactions. The induction of immune suppression in immunized animals is also correlated with a shift of cytokine balance, as reflected by an elevated level of IL-10 and reduced level of IFN-gamma or IL-2. Hence, co-immunization with DNA- and protein-based vaccines may represent a novel means for inducing active suppression against untoward immunity.
Jin
Huali
H
State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing.
Kang
Youmin
Y
Zheng
Guoxing
G
Xie
Qifa
Q
Xiao
Chong
C
Zhang
Xinyu
X
Yu
Yang
Y
Zhu
Kaichun
K
Zhao
Gan
G
Zhang
Fuchun
F
Chen
Aoshuang
A
Wang
Bin
B
D000818:Animals; D007111:Immunity, Cellular; D007114:Immunization; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology
D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D007371:Interferon-gamma