Indole alkaloids from Tabernaemontana australis (Muell. Arg) Miers that inhibit acetylcholinesterase enzyme.
Ten indole alkaloids from the chloroform extract of stalk of Tabernaemontana australis (Muell. Arg) Miers were tentatively identified by GC-MS, viz., coronaridine (1), voacangine (2), voacangine hydroxyindolenine (3), rupicoline (4), ibogamine (5), ibogaine (6), ibogaline (7), desethyl-voacangine (8), voachalotine (9), and affinisine (10). Of these, the first four were isolated by silica gel open column chromatography, identified by uni- and bidimensional NMR, IR, MS and showed anti-cholinesterasic activity at the same concentration as the reference compounds physostigmine and galanthamine (detection limit of 0.01mM) by TLC assay using the modified Ellman's method.
Andrade
Marcelo T
MT
Instituto de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco A, Cidade Universitária, 21945-970 Rio de Janeiro, RJ, Brazil.
Lima
Josélia A
JA
Pinto
Angelo C
AC
Rezende
Claudia M
CM
Carvalho
Meriane P
MP
Epifanio
Rosângela A
RA
D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D002845:Chromatography; D004524:Eels; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D013057:Spectrum Analysis; D030014:Tabernaemontana / Q000737:chemistry*
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007211:Indoles; D010936:Plant Extracts