The research on biotransformation of praziquantel.
Praziquantel (PZQ), a broad spectrum antihelmintic drug, is extensively metabolized in the liver, yielding mainly monohydroxylated and dihydroxylated phase-I-metabolites. However, the exact chemical structures of the most metabolites are still unknown. In the presented research, three types of PZQ biotransformations were performed: biotransformation with the whole cells of Saccharomyces cerevisiae, with cytochrome c from Saccharomyces cerevisiae and with microsomes isolated from Saccharomyces cerevisiae. To describe the obtained metabolites TLC, RP-TLC, and HPLC were used.
Godawska-Matysik
Anna
A
Jagiellonian University Medical College, Department of Technology and Biotechnology of Drugs, 9 Medyczna Str., PL 30-688 Kraków.
Pekala
Elzbieta
E
Kieć-Kononowicz
Katarzyna
K
D000980:Antiplatyhelmintic Agents / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D045304:Cytochromes c / Q000378:metabolism; D007202:Indicators and Reagents; D011223:Praziquantel / Q000493:pharmacokinetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D000980:Antiplatyhelmintic Agents; D007202:Indicators and Reagents; D011223:Praziquantel; D045304:Cytochromes c