Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection.
A sensitive and accurate analytical method for the determination of ochratoxin A (OTA) in rice, based on extraction with phosphate-buffered saline/methanol, an immunoaffinity column (IAC) for clean-up, and high performance liquid chromatography with fluorescence detection (HPLC-FD), is described. The limit of quantification of the proposed method was 0.05 microg kg(-1). Recovery of OTA from rice samples spiked at 0.05 microg kg(-1) was 92%, with a within-day RSD of 5.4%. The proposed method was applied to 42 rice samples from Portugal and the presence of OTA was found in six samples at concentrations ranging from 0.09 to 3.52 microg kg(-1). The identification of OTA was confirmed by methyl ester derivatization and then HPLC analysis. The daily intake of OTA by the Portuguese population was also estimated.
Pena
A
A
Group of Bromatology, Faculty of Pharmacy, University of Coimbra, 3049 Coimbra, Portugal. apena@ci.uc.pt
Cerejo
F
F
Lino
C
C
Silveira
I
I
D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D011174:Portugal; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D009793:Ochratoxins; C025589:ochratoxin A