title: Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus thuringiensis.
abstract: The expression of vip3LB, one of the vegetative insecticidal protein-encoding genes of Bacillus thuringiensis, was studied at the transcriptional level. By primer extension analysis, we have identified, for the first time, the transcription start point of vip3-type gene. Upstream from vip3LB transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E sigma(E) holoenzyme of B. subtilis. Thus, it was strongly suggested that the identified vip3 promoter was under the control of sigma(35)-like enzyme, the B. thuringiensis homolog of sigma(E). The transcriptional activity from the promoter was detected at the vegetative stage of growth starting at mid-log phase as well as during the middle stage of sporulation.
authors.affiliation: Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Lobna Abdelkefi
authors.initials: LA
authors.lastname: Mesrati
authors.forename: Slim
authors.initials: S
authors.lastname: Tounsi
authors.forename: Fakher
authors.initials: F
authors.lastname: Kamoun
authors.forename: Samir
authors.initials: S
authors.lastname: Jaoua
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D024363:Transcription Initiation Site
chemical_list: D001426:Bacterial Proteins