title: A functional polymorphism in the IL-10 promoter influences the response after vaccination with HBsAg and hepatitis A.
abstract: The immune response to hepatitis B surface antigen (HBsAg) is mostly genetically determined. Interleukin 10 (IL-10) is a central immunoregulatory cytokine with important effects on B-cells. We have studied the influence of IL-10 promoter polymorphisms on the immune response to HBsAg and hepatitis A vaccination. We vaccinated 202 twin pairs in an open prospective study with a combined recombinant HBsAg/inactivated hepatitis A vaccine. IL-10 promoter polymorphisms were investigated in all individuals and their influence on anti-HBs, and anti-HAV responsiveness was studied. In the multiple regression analysis accounting for smoking, gender, body mass index and age, the ACC haplotype (-1082, -819 and -592) had a strong influence on anti-HBs production. Individuals carrying the ACC haplotype had anti-HBs titres almost twice as high as individuals without this haplotype. In contrast, anti-HAV production was suppressed by the presence of the -1082A allele in comparison with individuals homozygous for the -1082G allele. The contribution of the shared IL-10 promoter haplotype accounted for 27% of the genetic influence on anti-HBs antibody response. In conclusion, genetic variability in the IL-10 promoter is an important modulator of the immune response against hepatitis viral antigens.
authors.affiliation: Department of Internal Medicine, Johannes Gutenberg University, Mainz, Germany. thomas.hoehler@prosper-hospital.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Höhler
authors.forename: Esther
authors.initials: E
authors.lastname: Reuss
authors.forename: Christine M
authors.initials: CM
authors.lastname: Freitag
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schneider
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D005260:Female; D022362:Hepatitis A Vaccines / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D008297:Male; D011110:Polymorphism, Genetic / Q000276:immunology*; D011401:Promoter Regions, Genetic; D011446:Prospective Studies; D014427:Twins
chemical_list: D000956:Antigens, Viral; D022362:Hepatitis A Vaccines; D006514:Hepatitis B Surface Antigens; D016753:Interleukin-10