Inhibition of gastric cancer-associated angiogenesis by antisense COX-2 transfectants.
We characterized the effects of selective Cox-2 inhibition on the angiogenesis gastric cancer cell line SGC7901 by stable transfection of antisense Cox-2 cDNA (Cox-2-AS) or pharmacological inhibition by selective cox-2 inhibitor (NS398). The conditioned media obtained from SGC7901 treated with Cox-2-AS or NS398 suppressed the proliferation, migration and tube formation of human umbilical vein endothelial cells. Moreover, both treatments inhibited in vivo angiogenesis. These inhibitions could be partially reversed by the addition of PGE2. Our findings support that selective inhibition of Cox-2 alone plays an instrumental role on gastric cancer associated angiogenesis.
Fu
Yuan-Gen
YG
Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital 30-32 Ngan Shing Street, Shatin N.T., Hong Kong, SAR, China.
Sung
Joseph Jy
JJ
Wu
Kai-Chun
KC
Wu
Han-Pin
HP
Yu
Jun
J
Chan
Martin
M
Chan
Victor Yw
VY
Chan
Ka-Kui
KK
Fan
Dai-Ming
DM
Leung
Wai K
WK
D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D016373:DNA, Antisense; D018076:DNA, Complementary; D006801:Humans; D008565:Membrane Proteins; D009389:Neovascularization, Pathologic; D009578:Nitrobenzenes / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000473:pathology*; D013449:Sulfonamides / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured
D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D016373:DNA, Antisense; D018076:DNA, Complementary; D008565:Membrane Proteins; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases