Identification of Helicobacter pylori surface proteins by selective proteinase K digestion and antibody phage display.
Five surface proteins of Helicobacter pylori were identified by proteinase K treatment of live H. pylori followed by proteome analysis. One of the identified proteins, HopQ, is also recognized by an antibody selected by phage display screening of intact H. pylori.
Sabarth
Nicolas
N
Max Planck Institute for Infection Biology, Department of Molecular Biology, Schumannstrasse 21/22, D-10117 Berlin, Germany. nicolas.2.sabarth@gsk.com
Hurvitz
Robert
R
Schmidt
Monika
M
Zimny-Arndt
Ursula
U
Jungblut
Peter R
PR
Meyer
Thomas F
TF
Bumann
Dirk
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D019286:Endopeptidase K; D016480:Helicobacter pylori / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D040901:Proteomics
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D019151:Peptide Library; D019286:Endopeptidase K