[Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence].
OBJECTIVE
To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence.


METHODS
Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining.


RESULTS
At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells.


CONCLUSIONS
Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.
Yao
Qi-sheng
QS
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China. hbsyyqsdr@163.com
Ye
Zhang-qun
ZQ
Chen
Cong-bo
CB
Wang
Xiao-kang
XK
Wang
Wei-min
WM
Chen
Li-xin
LX
D000818:Animals; D002470:Cell Survival; D017690:Cell Transplantation; D005260:Female; D018482:Muscle, Skeletal / Q000166:cytology*; D011183:Postoperative Complications / Q000628:therapy*; D011468:Prostatectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous; D014521:Urethra / Q000166:cytology; D001743:Urinary Bladder / Q000166:cytology; D014549:Urinary Incontinence / Q000209:etiology / Q000628:therapy*; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase