[Distribution of Fas and FasL in the central nervous system of adult rhesus].
OBJECTIVE
To investigate the distribution of Fas and FasL in the CNS of adult rhesus.


METHODS
Frozen sections were incubated in polyclonal anti-Fas and anti-FasL antibody by the immunohistochemical SP method.


RESULTS
The Fas and FasL immunopositive neurons were observed in many areas. Fas immunoreactivity could be seen in the cytoplasm and processes of Purkinje cells and in the brain stem nuclei, including vestibular nucleus, dorsal nucleus of vagus and spinal nucleus of trigeminal nerve. FasL immunopositive neurons were observed in cerebral cortex, especially in pyramidal neurons of lamina I and V, cerebellar nuclei, diencephalon, and brain stem nuclei involving pontine nucleus, vestibular nucleus, cochlear nucleus, spinal nucleus of trigeminal nerve, hypoglossal nucleus, nucleus ambiguous and reticular formation. Fas and FasL immunoreactivity mainly distributed in motor neurons of spinal ventral horn and neural fibers and glia cells in white matter. They all took on brown staining in the cytoplasm and process.


CONCLUSIONS
The distribution profiles of Fas and FasL in various areas of CNS indicate that they may fill some roles in the immune and physical function of the aforesaid anatomic
Guan
Yu-guang
YG
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Wang
Ting-hua
TH
Ni
Wei
W
Li
Lang
L
Lu
Yong-chao
YC
Gao
Zhi-yu
ZY
D000818:Animals; D001923:Brain Chemistry; D001933:Brain Stem / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D053222:Fas Ligand Protein; D008253:Macaca mulatta; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D013116:Spinal Cord / Q000737:chemistry; D014018:Tissue Distribution; D048069:Tumor Necrosis Factors / Q000378:metabolism*; D014726:Vestibular Nuclei / Q000737:chemistry; D019014:fas Receptor / Q000378:metabolism*
D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors; D019014:fas Receptor