title: Two families with nonsyndromic low-frequency hearing loss harbor novel mutations in Wolfram syndrome gene 1.
abstract: Although hereditary hearing loss is highly heterogeneous, only a few loci have been implicated with low-frequency hearing loss. Mutations in one single gene, Wolfram syndrome 1 (WFS1), have been reported to account for most familial cases with this type of hearing impairment. This study was conducted to determine the cause of nonsyndromic low-frequency hereditary hearing impairment in two large families. Two large families from Switzerland and United States with low-frequency hearing loss were identified. Genomewide linkage analysis was performed followed by mutation screening in the candidate gene WFS1 with direct DNA sequencing and restriction fragment analysis. Both families were linked to DFNA6/14/38 with lod scores>3. Two novel heterozygous missense mutations in WFS1 were identified: c.2311G>C leading to p.D771H in the Swiss family and c.2576G>C leading to p.R859P in the US family. The sequence alteration was absent in 100 control chromosomes. Nonsyndromic low-frequency hereditary hearing impairment seems to be predominantly a monogenic disorder due to WFS1. We confirm that most mutations in WFS1 associated with isolated low-frequency hearing loss are clustered in the C-terminal protein domain coded by exon 8.
authors.affiliation: Hals-Nasen-Ohren-Klinik, Kantonsspital Aarau, Aarau, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gürtler
authors.forename: Yuil
authors.initials: Y
authors.lastname: Kim
authors.forename: Anand
authors.initials: A
authors.lastname: Mhatre
authors.forename: Christoph
authors.initials: C
authors.lastname: Schlegel
authors.forename: Adolf
authors.initials: A
authors.lastname: Mathis
authors.forename: Robert
authors.initials: R
authors.lastname: Daniels
authors.forename: Clough
authors.initials: C
authors.lastname: Shelton
authors.forename: Anil K
authors.initials: AK
authors.lastname: Lalwani
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D002648:Child; D004252:DNA Mutational Analysis; D005260:Female; D008040:Genetic Linkage; D034381:Hearing Loss / Q000235:genetics* / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013557:Switzerland; D014589:Utah
chemical_list: D008565:Membrane Proteins; C114987:wolframin protein