Offspring resulting from transfer of cryopreserved embryos in camel (Camelus dromedarius).
The dromedary embryos, collected at hatched blastocyst stage, survived freezing and thawing in the presence of a high concentration of ethylene glycol (7.0 mol/L) with sucrose (0.5 mol/L) and direct plunging in liquid nitrogen. The rate of survival, as judged by the morphological appearance of the embryos after thawing, was high (92%). The transfer of frozen-thawed embryos into the recipients during the breeding (n=20) and non-breeding season (n=25) resulted in two and one pregnancy, respectively. One of the two pregnant recipients, with embryos transferred during the breeding season, delivered a normal healthy male calf at term. To our knowledge, this offspring is the first camelid produced following transfer of a frozen-thawed embryo.
Nowshari
Manzoor A
MA
Central Veterinary Research Laboratory, P.O. Box 597, Dubai, United Arab Emirates. mnowshari@cvrl.ae
Ali
Syed A
SA
Saleem
Shazia
S
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D001947:Breeding; D002162:Camelus; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D004622:Embryo, Mammalian / Q000502:physiology*; D019855:Ethylene Glycol; D005260:Female; D011247:Pregnancy; D012621:Seasons; D013395:Sucrose; D014021:Tissue Preservation / Q000662:veterinary*; D020858:Tissue and Organ Harvesting / Q000662:veterinary
D013395:Sucrose; D019855:Ethylene Glycol