title: Capillary electrophoresis assay of netilmicin sulphate.
abstract: An effective method based on capillary electrophoresis (CE) for the determination of netilmicin sulphate in commercial ophthalmic formulations was developed and validated. The use of a polymer-coated capillary and a non-absorbing running buffer permitted the elution of netilmicin in cationic mode at pH 3.0 and with direct UV detection at lambda=195 nm. Since pre-treatment of the samples is not required, this procedure may be straightforwardly applied to the other aminoglycosides provided that their extinction coefficient is not too low.
authors.affiliation: Sifi SpA, Via E. Patti, 36 Lavinaio (CT) 95020, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Calcara
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Enea
authors.forename: Angelo
authors.initials: A
authors.lastname: Pricoco
authors.forename: Fausto
authors.initials: F
authors.lastname: Miano
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009428:Netilmicin / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D011092:Polyethylene Glycols; D009428:Netilmicin; D011142:Polyvinyl Alcohol