A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits.
A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits was compared with a conventional ELISA. This whole-blood ELISA has advantages. The volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. The incubation time was shortened to 5 minutes. Wells were washed three to five times with PBS-Tween after each procedure. Optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. Constant temperature was not necessary. The entire procedure took only 20-30 minutes.
Hirose
Yoshinori
Y
Department of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.
Kirinoki
Masashi
M
Chigusa
Yuichi
Y
Matsuda
Hajime
H
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008403:Mass Screening / Q000379:methods; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis*; D013997:Time Factors
D000947:Antigens, Helminth