title: Identification of adrenomedullin in avian type II pneumocytes: increased expression after exposure to air pollutants.
abstract: Adrenomedullin (AM) is a potent vasodilator peptide present in the lung of mammals where it is expressed mainly in the columnar epithelium and alveolar macrophages. AM increases the secretion of phosphatidylcholine by type II pneumocytes, which suggests a role as an autocrine modulator of surfactant secretion. In this study we show the expression of an AM-like protein in the lung of the pigeon, Columba livia. Using an antibody against its human ortholog, AM-like immunoreactivity was found to be associated with membranous structures of the multivesicular bodies of type II pneumocytes. We also studied the differential expression of AM-like peptide in the lung of pigeons exposed to polluted city air vs cleaner countryside conditions and found that AM-like expression was higher in city animals. Similar results were obtained in an experimental study in which pigeons were exposed to increasing concentrations of a single pollutant, ozone. Taken together, our findings support the implication of AM in the response of type II pneumocytes to air pollutants.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA. ncuesta@som.umaryland.edu.
authors.forename: Natalia
authors.initials: N
authors.lastname: Cuesta
authors.forename: Alfredo
authors.initials: A
authors.lastname: Martínez
authors.forename: Frank
authors.initials: F
authors.lastname: Cuttitta
authors.forename: Enrique
authors.initials: E
authors.lastname: Zudaire
mesh_terms: D053607:Adrenomedullin; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D010856:Columbidae; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010126:Ozone / Q000633:toxicity; D010455:Peptides / Q000819:agonists* / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D010455:Peptides; D053607:Adrenomedullin; D010126:Ozone