Measuring T-cell function in animal models of tuberculosis by ELISPOT.
Enzyme-linked immunospot (ELISPOT) was originally developed from an enzyme-linked immunosorbent assay (ELISA) to detect and measure the frequency of individual cells that produce cytokines in response to antigenic stimulation. ELISPOT assay is more sensitive than the enzyme-linked immunosorbent assay or intracellular cytokine staining. Increased sensitivity of ELISPOT is particularly advantageous when studying T-cell-mediated responses in Mycobacterium tuberculosis infection because antigen specific T-cells can occur at a low frequency in vivo. This method has been successfully used to analyze M. tuberculosis immune responses in humans in addition to murine and nonhuman primate models of tuberculosis.
Lazarevic
Vanja
V
Dept. of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA, USA.
Pawar
Santosh
S
Flynn
Joanne
J
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000882:Haplorhini; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D007371:Interferon-gamma