title: A study of interlaboratory influence on column evaluation.
abstract: A liquid chromatography method for the characterization of base deactivated columns was investigated in a collaborative study involving six laboratories. This work was carried out on two chromatographic supports (Xterra RP 18 and Symmetry Shield). Different cooling systems, namely water bath and air oven, were tested and it was shown that column thermoregulation did not significantly influence chromatographic data. In order to control the mobile phase composition, the latter was prepared by weight rather than volume. Thanks to the injection of a set of selected neutral compounds, extra-column effects were evaluated in each of the participating laboratories. The results showed that chromatographic supports tested in different laboratories and following the same test protocol could be effectively compared.
authors.affiliation: Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, Switzerland.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Stella
authors.forename: Sabine
authors.initials: S
authors.lastname: Heinisch
authors.forename: Xiangli
authors.initials: X
authors.lastname: Liu
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Carrupt
authors.forename: Gabriel
authors.initials: G
authors.lastname: Cazorla
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Gauvrit
authors.forename: Pierre
authors.initials: P
authors.lastname: Lantéri
authors.forename: Katell Le
authors.initials: KL
authors.lastname: Mapihan
authors.forename: Jérôme
authors.initials: J
authors.lastname: Vial
authors.forename: Sylvie
authors.initials: S
authors.lastname: Héron
authors.forename: Alain
authors.initials: A
authors.lastname: Tchapla
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Rocca
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007753:Laboratories