[A study of the association of iNOS and eNOS gene polymorphism with portal hypertension in liver cirrhosis].
OBJECTIVE
To study whether polymorphism of iNOS and eNOS genes is associated with portal hypertension in liver cirrhosis.


METHODS
A case control study of 106 patients with liver cirrhosis due to HBV was performed in comparison with 108 controls using PCR-RFLP to detect iNOS promoter -969G --> C and eNOS exon7 894G --> T polymorphism.


RESULTS
The frequency of the C allele and GC genotype in the iNOS promoter -969G --> C was significantly higher in the portal hypertension group than in the control group. (P < 0.05). The frequency of the T allele and GT genotype in eNOS exon7 894G --> T was dramatically higher in the portal hypertension group than in the control group (P < 0.05). Multivariate logistic regression analysis revealed that iNOS polymorphism in iNOS promoter -969G --> C and eNOS exon7 894G --> T was an independent new risk factor for portal hypertension. We discovered that iNOS promoter -969G --> C led to an increase in its functional activity.


CONCLUSIONS
The polymorphism of iNOS promoter -969G --> C and eNOS exon7 894G --> T is associated with portal hypertension of liver cirrhosis, which is a new independent risk factor found related to the occurrence of portal hypertension. The polymorphism of iNOS promoter -969G --> C results in functional activity increase of the iNOS promoter.
Cheng
Yuan-qiao
YQ
Department of Infectious Diseases, Dongguan People's Hospital, Guangdong Province, Dongguan 523018, China.
Lin
Ju-sheng
JS
Wang
Wen-qi
WQ
Xiong
Ping
P
Jiang
Xiao-dan
XD
D000328:Adult; D005260:Female; D019694:Hepatitis B, Chronic / Q000150:complications; D006801:Humans; D006975:Hypertension, Portal / Q000209:etiology / Q000235:genetics*; D008103:Liver Cirrhosis / Q000150:complications* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics
D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III