Apoptosis induction on HL-60 cells of a novel polysaccharide from the mucus of the loach, Misgurnus anguillicaudatus.
This study was designed to determine the anti-proliferative, apoptotic properties of a novel polysaccharide from the loach, Misgurnus anguillicaudatus (MAP), using a human promyelocytic leukemia line (HL-60) as a model system. HL-60 cells were cultured in the presence of MAP at various concentrations (50-800 mg/l) for 5 days and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results showed that MAP inhibited the cells viability in time and concentration-dependent characteristics. We found that anti-proliferative effect of MAP was associated with apoptosis on HL-60 cells by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, the content of nitric oxide (NO) and activity of lactate dehydrogenase (LDH) release increased when the cells incubated with MAP at various concentrations and times. These investigations suggest that the polysaccharide from loach has the function of anti-proliferation and induction of apoptosis in tumor cells in vitro.
Zhang
Chen-Xiao
CX
Department of Chemistry, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, PR China.
Huang
Kai-Xun
KX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003531:Cypriniformes / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009093:Mucus / Q000737:chemistry* / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D009573:Nitrites; D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase