title: Plasma 25-hydroxy vitamin D concentrations, vitamin D receptor genotype and breast cancer risk in a UK Caucasian population.
abstract: Low levels of 25-hydroxy vitamin D (25(OH)D) and polymorphisms in the vitamin D receptor gene (VDR) have been found separately to increase risk of breast cancer. The aim of this study was to determine whether low 25(OH)D levels, alone and in combination with BsmI VDR genotype, increased breast cancer risk in a United Kingdom (UK) Caucasian population. Breast cancer patients (n=179) and control women (n=179) were recruited and 25(OH)D levels measured by enzyme-linked immunosorbent assay (ELISA). VDR genotype was determined by polymerase chain reaction (PCR) and restriction enzyme digest. Analysis showed that subjects with 25(OH)D levels <50 nM and the bb BsmI VDR genotype are 6.82 times more likely to have breast cancer than subjects with levels of 25(OH)D>50 nM and either the BB or Bb genotype (95% confidence interval (CI) 2.31-14.7, P<0.001). This study indicates that low levels of circulating 25(OH)D, both alone and in combination with BsmI VDR genotype, may increase risk of breast cancer in a UK Caucasian population.
authors.affiliation: Department of Cellular and Molecular Medicine, St. George's Hospital Medical School, Cranmer Terrace, London SW17 ORE, UK.
authors.forename: Lorraine C
authors.initials: LC
authors.lastname: Lowe
authors.forename: Michelle
authors.initials: M
authors.lastname: Guy
authors.forename: Janine L
authors.initials: JL
authors.lastname: Mansi
authors.forename: Clare
authors.initials: C
authors.lastname: Peckitt
authors.forename: Judith
authors.initials: J
authors.lastname: Bliss
authors.forename: Rosalind Given
authors.initials: RG
authors.lastname: Wilson
authors.forename: Kay W
authors.initials: KW
authors.lastname: Colston
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000097:blood* / Q000208:ethnology / Q000235:genetics; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics*; D012307:Risk Factors; D006113:United Kingdom; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood*; D044465:White People
chemical_list: D018167:Receptors, Calcitriol; D014807:Vitamin D; C104450:25-hydroxyvitamin D