Specificity of trypsin and chymotrypsin: loop-motion-controlled dynamic correlation as a determinant.
Trypsin and chymotrypsin are both serine proteases with high sequence and structural similarities, but with different substrate specificity. Previous experiments have demonstrated the critical role of the two loops outside the binding pocket in controlling the specificity of the two enzymes. To understand the mechanism of such a control of specificity by distant loops, we have used the Gaussian network model to study the dynamic properties of trypsin and chymotrypsin and the roles played by the two loops. A clustering method was introduced to analyze the correlated motions of residues. We have found that trypsin and chymotrypsin have distinct dynamic signatures in the two loop regions, which are in turn highly correlated with motions of certain residues in the binding pockets. Interestingly, replacing the two loops of trypsin with those of chymotrypsin changes the motion style of trypsin to chymotrypsin-like, whereas the same experimental replacement was shown necessary to make trypsin have chymotrypsin's enzyme specificity and activity. These results suggest that the cooperative motions of the two loops and the substrate-binding sites contribute to the activity and substrate specificity of trypsin and chymotrypsin.
Ma
Wenzhe
W
Center for Theoretical Biology, College of Chemistry, Peking University, Beijing, China.
Tang
Chao
C
Lai
Luhua
L
D000595:Amino Acid Sequence; D001665:Binding Sites; D002918:Chymotrypsin / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D003198:Computer Simulation; D004789:Enzyme Activation; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009038:Motion; D011485:Protein Binding; D011487:Protein Conformation; D020539:Sequence Analysis, Protein / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014357:Trypsin / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*
D002918:Chymotrypsin; D014357:Trypsin