title: A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii.
abstract: BACKGROUND
  The role of centrioles in mitotic spindle function remains unclear. One approach to investigate mitotic centriole function is to ask whether mutation of centriole-associated proteins can cause genomic instability.
  
  
  RESULTS
  We addressed the role of the centriole-associated EF-hand protein centrin in genomic stability using a Chlamydomonas reinhardtii centrin mutant that forms acentriolar bipolar spindles and lacks the centrin-based rhizoplast structures that join centrioles to the nucleus. Using a genetic assay for loss of heterozygosity, we found that this centrin mutant showed increased genomic instability compared to wild-type cells, and we determined that the increase in genomic instability was due to a 100-fold increase in chromosome loss rates compared to wild type. Live cell imaging reveals an increased rate in cell death during G1 in haploid cells that is consistent with an elevated rate of chromosome loss, and analysis of cell death versus centriole copy number argues against a role for multipolar spindles in this process.
  
  
  CONCLUSIONS
  The increased chromosome loss rates observed in a centrin mutant that forms acentriolar spindles suggests a role for centrin protein, and possibly centrioles, in mitotic fidelity.
authors.affiliation: Dept. of Biochemistry & Biophysics, University of California-San Francisco, 600 16th St., San Francisco, California, USA. dsjiz@itsa.ucsf.edu
authors.forename: Ivan
authors.initials: I
authors.lastname: Zamora
authors.forename: Wallace F
authors.initials: WF
authors.lastname: Marshall
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D002502:Centrioles / Q000235:genetics*; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D043171:Chromosomal Instability / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000502:physiology; D002872:Chromosome Deletion; D009154:Mutation
chemical_list: D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; C056608:caltractin