Microbial transformation of 20(S)-protopanaxatriol by Mucor spinosus.
Microbial biotransformation of 20(S)-protopanaxatriol (1) by the fungus Mucor spinosus (AS 3.3450) yielded four new metabolites, the structures of which were determined to be 12-oxo-15alpha-hydroxyl-20(S)-protopanaxatriol (2), 27-hydroxyl-20(S)-protopanaxatriol (3), 12-oxo-26-hydroxyl-20(S)-protopanaxatriol (4), and 12-oxo-27-hydroxyl-20(S)-protopanaxatriol (5), respectively, on the basis of their chemical and spectroscopic data. All the new metabolites, as well as the substrate, had significant cytotoxic effects on HL-60 cells (human leukemia cells).
Tian
Yin
Y
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road #38, Beijing 100083, People's Republic of China.
Guo
Hongzhu
H
Han
Jian
J
Guo
Dean
D
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001711:Biotransformation; D004354:Drug Screening Assays, Antitumor; D015394:Molecular Structure; D009089:Mucor / Q000378:metabolism*; D012502:Sapogenins / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism; D014315:Triterpenes / Q000378:metabolism* / Q000494:pharmacology
D000970:Antineoplastic Agents; D012502:Sapogenins; D014315:Triterpenes; C081552:protopanaxatriol