Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice.
Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have recently reported that adipose-derived stromal cells (ASCs) could differentiate into mesenchymal lineages in vitro. In this study, we performed neural induction using ASCs from GFP transgenic mice and were able to induce these ASCs into neuronal and glial cell lineages. Most of the neurally induced cells showed bipolar or multipolar appearance morphologically and expressed neuronal markers. Electron microscopy revealed their neuronal morphology. Some cells also showed glial phenotypes, as shown immunocytochemically. The present study clearly shows that ASCs derived from GFP transgenic mice differentiate into neural lineages in vitro, suggesting that these cells might provide an ideal source for further neural stem cell research with possible therapeutic application for neurological disorders.
Fujimura
Juri
J
Department of Pharmacology, Nippon Medical School, Tokyo, Japan. juri-f@nms.ac.jp
Ogawa
Rei
R
Mizuno
Hiroshi
H
Fukunaga
Yoshitaka
Y
Suzuki
Hidenori
H
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D048429:Cell Size; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D049452:Green Fluorescent Proteins