Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells.
The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5'-triphosphate (ATP), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non-migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relationship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.
Mao
Jian Wen
JW
Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, China.
Wang
Li Wei
LW
Jacob
Tim
T
Sun
Xue Rong
XR
Li
Hui
H
Zhu
Lin Yan
LY
Li
Pan
P
Zhong
Ping
P
Nie
Si Huai
SH
Chen
Li Xin
LX
D000255:Adenosine Triphosphate / Q000378:metabolism; D020533:Angiogenesis Inhibitors / Q000378:metabolism; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D018118:Chloride Channels / Q000378:metabolism; D006801:Humans; D007038:Hypotonic Solutions; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D009579:Nitrobenzoates / Q000378:metabolism; D013629:Tamoxifen / Q000378:metabolism; D014407:Tumor Cells, Cultured
D020533:Angiogenesis Inhibitors; D018931:Antineoplastic Agents, Hormonal; D018118:Chloride Channels; D007038:Hypotonic Solutions; D009579:Nitrobenzoates; D013629:Tamoxifen; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D000255:Adenosine Triphosphate