Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor.
The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A resolution in-house and belongs to space group P2(1) with unit-cell parameters a=64.10 A, b=34.88 A, c=114.72 A, beta=90.49 degrees .
Duan
Ming-Rui
MR
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, Peking University, Beijing 100871, P.R. China.
Ren
Hui
H
Mao
Peng
P
Wei
Chun-Hong
CH
Liang
Yu-He
YH
Li
Yi
Y
Su
Xiao-Dong
XD
D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D004247:DNA