Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens.
Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes.
Min
Wongi
W
Animal Parasitic Diseases Laboratory, Animal & Natural Resources Institute, US Dept. of Agriculture, Beltsville, MD 20705, USA.
Lillehoj
Hyun S
HS
Ashwell
Christopher M
CM
van Tassell
Curtis P
CP
Dalloul
Rami A
RA
Matukumalli
Lakshmi K
LK
Han
Jae Y
JY
Lillehoj
Erik P
EP
D000818:Animals; D002645:Chickens / Q000235:genetics / Q000276:immunology / Q000469:parasitology*; D003048:Coccidiosis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D004539:Eimeria; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D019410:Interleukin-16 / Q000235:genetics; D020381:Interleukin-17 / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology; D008214:Lymphocytes / Q000378:metabolism*; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000469:parasitology*
D019410:Interleukin-16; D020381:Interleukin-17