Study of muscle regeneration using in vitro 2D 1H spectroscopy.
The in vivo spectrum of regenerating muscles shows a specific cross-correlation signal assigned to the (n-3) fatty acyl chain, which peaks during the myoblast fusion phase. In order to identify the origin of this signal and to take all the lipid metabolites into account, we investigated the degeneration-regeneration process by 1H 2D NMR of lipid muscle extracts. We observed an increase in the total amount of lipids during the regeneration process, although the lipid profile did not show any drastic change during this process. The changes in the NMR signal observed in vivo and, in particular, the appearance of the specific (n-3) fatty acyl chain signal appears to arise from mobile lipid compartments located in fusing cells.
Gillet
B
B
Institut de Chimie des Substances Naturelles UPR 2301, CNRS, 91198 Gif sur Yvette cedex, France. gillet@icsn.cnrs-gif.fr
Sebrié
C
C
Bogaert
A
A
Bléneau
S
S
de la Porte
S
S
Beloeil
J-C
JC
D000818:Animals; D005121:Extremities / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006859:Hydrogen; D008055:Lipids / Q000737:chemistry / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology*; D032446:Myoblasts / Q000737:chemistry / Q000502:physiology; D012016:Reference Values; D012038:Regeneration / Q000502:physiology*; D014020:Tissue Extracts / Q000737:chemistry
D008055:Lipids; D014020:Tissue Extracts; D006859:Hydrogen