Promotion of stable triplex formation by partial incorporation of 2',5'-phosphodiester linkages into triplex-forming oligonucleotides.
Pentadecamer homopyrimidine oligonucleotides containing three or more 2',5'-phosphodiester linkages in different modes were prepared and used to evaluate the ability as a triplex-forming oligonucleotide (TFO), and it was found that discontinuous replacement of the 3',5'-phosphodiester linkages in TFO by 2',5'-linkages significantly stabilizes parallel-motif triplexes.
Obika
Satoshi
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hiroto
Akiko
A
Nakagawa
Osamu
O
Imanishi
Takeshi
T
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D004951:Esterification; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry*; D013696:Temperature
D009841:Oligonucleotides; C051802:triplex DNA; D010758:Phosphorus; D004247:DNA