Rapid determination of ultra trace amounts of residual protein in penicillin based on its enhancement effects of Rayleigh light scattering on trimethoxyphenlyfluorone (TM-PF)-Mo(VI) complex.
This is a report on the ultra trace amount determination of protein in penicillin based on the interaction with trimethoxyphenlyfluorone (TM-PF)-molybdenum (Mo)(VI) complex in the presence of Triton X-100 microemulsion by Rayleigh light scattering (RLS). At pH 2.70, the weak RLS of TM-PF-Mo(VI) complex was enhanced greatly by the addition of proteins. Based on this, the interaction of TM-PF-Mo(VI) complex with proteins was studied and a new quantitative determination method for proteins was developed. This method is extremely sensitive (the linear range of the calibration curve was 0-80 ng mL(-1) for bovine serum albumin (BSA). The detection limit was 0.47 ng mL(-1)), simple, steady and free from interference of coexisting substances. TM-PF-Mo(VI) complex as a spectral probe can be used to the determination of the residual protein in penicillin samples successfully.
Qin
Wei
W
School of Chemistry and Chemical Engineering, Jinan University, Jinan 250022, PR China. sdjndxwq@263.net
Yan
Li
L
Hui
Zhang
Z
Yulong
Ding
D
Bin
Du
D
D000818:Animals; D002138:Calibration; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D000431:Ethanol / Q000494:pharmacology; D005452:Fluoresceins / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008027:Light; D008982:Molybdenum / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis* / Q000737:chemistry; D011489:Protein Denaturation / Q000187:drug effects; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
D005452:Fluoresceins; D009942:Organometallic Compounds; D010406:Penicillins; D011506:Proteins; D012710:Serum Albumin, Bovine; D000431:Ethanol; C010159:fluorone Black; D008982:Molybdenum; D017830:Octoxynol