title: Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cells.
abstract: We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Light Hall, Nashville, Tennessee 37232, USA.
authors.forename: Subhadra C
authors.initials: SC
authors.lastname: Gunawardana
authors.forename: Manami
authors.initials: M
authors.lastname: Hara
authors.forename: Graeme I
authors.initials: GI
authors.lastname: Bell
authors.forename: W Steven
authors.initials: WS
authors.lastname: Head
authors.forename: Mark A
authors.initials: MA
authors.lastname: Magnuson
authors.forename: David W
authors.initials: DW
authors.lastname: Piston
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology* / Q000196:embryology*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D019076:Transgenes / Q000235:genetics
chemical_list: D049452:Green Fluorescent Proteins