Cell-cycle-dependent efficacy of photodynamic therapy with ATX-S10(Na).
Photodynamic therapy (PDT) is a useful strategy for treating various cancers. Details of the mechanisms of PDT have not been made clear yet. We intended to study the efficacy of PDT in relation to the cell cycle. HeLa S3 cells were synchronized by the thymidine block method. Cells in different cell cycle phases after release were treated with the water-soluble photosensitizer, ATX-S10(Na). The cellular viability after PDT was determined by the MTT assay. Intracellular levels of ATX-S10(Na) in different cell cycle phases were also determined. We found that cells in the S and G(2)/M phases were hypersensitive to PDT with ATX-S10(Na) in comparison with those in the G(1) phase, and that cellular levels of ATX-S10(Na) were increased in cells in the S and G(2)/M phases compared to those in the G(1) phase. We conclude that cellular ATX-S10(Na) levels differ among the different cell cycle phases, which is associated with the cell-cycle-dependent efficacy of PDT with ATX-S10(Na).
Sano
Munetaka
M
First Department of Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.
Furuta
Takahisa
T
Takahira
Kenichiro
K
Kajimura
Masayoshi
M
Hanai
Hiroyuki
H
Kohno
Eiji
E
Hirano
Toru
T
Hishida
Akira
A
D000704:Analysis of Variance; D002453:Cell Cycle / Q000187:drug effects*; D005260:Female; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000494:pharmacology*; D011336:Probability; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
C115550:ATX-S10; D011166:Porphyrins