title: Herpes simplex virus 1 inhibits apoptosis through a caspase-3-dependent pathway in primary cultures of cortical neuronal cells of fetal mice.
abstract: We could induce apoptosis in primary cultures of cortical neurons of fetal mice with ceramide or sorbitol. The induction was accompanied by an increase in caspase-3 (CAS-3) activity and depolarization of the inner mitochondrial membrane of neuronal cells which both could be reversed by Herpes simplex virus 1 (HSV-1) infection. We conclude tha HSV-1 infection inhibited the apoptosis, induced in neuronal cells by sorbitol or ceramide, via a CAS-dependent pathway.
authors.affiliation: Department of Neurology, Beijing Friendship Hospital-Affiliate of Capital University of Medical Sciences, Beijing 100050, PR China.
authors.forename: J W
authors.initials: JW
authors.lastname: Wang
authors.forename: D X
authors.initials: DX
authors.lastname: Wang
authors.forename: R J
authors.initials: RJ
authors.lastname: Wang
authors.forename: W R
authors.initials: WR
authors.lastname: Li
authors.forename: H Z
authors.initials: HZ
authors.lastname: Tuo
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Feng
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D013012:Sorbitol / Q000494:pharmacology
chemical_list: D061945:Caspase Inhibitors; D002518:Ceramides; D013012:Sorbitol; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases