STR-genotyping from human medieval tooth and bone samples.
We extracted the DNA contained in samples of bones and teeth from 10 skeletons excavated from the Gravette site (400-1000 AD, south of France). Ancient DNA was analysed by autosomal short tandem repeats (STRs). The DNA present in these ancient remains appeared very degraded, but nevertheless, better conserved in tooth than in bone samples. Moreover, we showed that the DNA extracted from ancient dental pulp was not exempt from polymerase chain reaction (PCR) inhibitors, which could result from extreme DNA fragmentation. An adapted protocol with a supplementary step of purification removed this inhibition.
Ricaut
François-Xavier
FX
Laboratoire d'Anthropologie Moléculaire, Institut de Médecine Légale, 11 rue Humann, 67085 Strasbourg Cedex, France. fx.ricaut@infonie.fr
Keyser-Tracqui
Christine
C
Crubézy
Eric
E
Ludes
Bertrand
B
D000328:Adult; D053523:Amelogenin; D001842:Bone and Bones / Q000473:pathology*; D002648:Child; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D016172:DNA Fingerprinting / Q000379:methods*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D018732:Forensic Anthropology; D005838:Genotype; D049691:History, Medieval; D006801:Humans; D008297:Male; D010163:Paleontology; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences; D014083:Tooth Germ / Q000473:pathology
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA