Anti-diabetic activity of beta-glucans and their enzymatically hydrolyzed oligosaccharides from Agaricus blazei.
Beta-glucans were prepared from Agaricus blazei Murill by repeated extraction with hot water. The average molecular weights of beta-glucans were 30-50 kDa by gel filtration chromatography. Oligosaccharides (AO), derived from hydrolyzing beta-glucans with an endo-beta-(1-->6)-glucanase from Bacillus megaterium, were mainly di- and tri-saccharides. Though beta-glucans and AO both showed anti-hyperglycemic, anti-hypertriglyceridemic, anti-hypercholesterolemic, and anti-arteriosclerotic activity indicating overall anti-diabetic activity in diabetic rats, AO had about twice the activity of beta-glucans with respect to anti-diabetic activity.
Kim
Yea-Woon
YW
Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University, Gimhae, 621-749, Republic of Korea.
Kim
Ki-Hoon
KH
Choi
Hyun-Ju
HJ
Lee
Dong-Seok
DS
D000364:Agaricus / Q000378:metabolism*; D000818:Animals; D001410:Bacillus megaterium / Q000201:enzymology; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood; D014312:Trisaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D047071:beta-Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D004187:Disaccharides; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D009844:Oligosaccharides; D014280:Triglycerides; D014312:Trisaccharides; D047071:beta-Glucans; D002784:Cholesterol; D006026:Glycoside Hydrolases; C020105:endo-1,6-beta-glucanase