Ultrastructural localization of laminin-5 (gamma2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy.
Laminin-5 (Ln-5) is an important molecule associated with epithelial cell adhesion and migration. In the gingiva around the tooth, Ln-5 localizes within basement membranes between the junctional epithelium (JE) and the tooth or connective tissue. Recently, we reported that in the oral mucosa around a dental implant, Ln-5 is expressed within the basement membranes at the implant-peri-implant epithelium (PIE) interface, and at the PIE-connective tissue interface. However, the ultrastructural localization of Ln-5 within or along the PIE has not yet been reported. Therefore, peri-implant oral mucosa was treated with anti-Ln-5 (gamma2 chain) antibody and examined using immuno-electron microscopy. Ln-5 was localized in the cells of the innermost-third layer and basal layer of the PIE. A 100-nm-wide Ln-5-positive internal basal lamina (basement membrane) and hemidesmosomes as adhesion structures were formed at the apical portion of the implant-PIE interface. However, at the upper-middle portion of the interface, these adhesion structures were not observed. Furthermore, at the PIE-connective tissue interface, the Ln-5-positive external basal lamina (basement membrane) and hemidesmosomes were partially deficient. Judging from these findings, we concluded that Ln-5 contributes to the attachment of the PIE to the titanium surface, and that PIE attached to titanium at the apical portion of the dental implant-PIE interface.
Atsuta
Ikiru
I
Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
Yamaza
Takayoshi
T
Yoshinari
Masao
M
Goto
Tetsuya
T
Kido
Mizuho A
MA
Kagiya
Tadayoshi
T
Mino
Satoya
S
Shimono
Masaki
M
Tanaka
Teruo
T
D000818:Animals; D001672:Biocompatible Materials; D015921:Dental Implants; D007797:Laminin / Q000378:metabolism* / Q000648:ultrastructure*; D008297:Male; D008422:Materials Testing; D016253:Microscopy, Immunoelectron; D009061:Mouth Mucosa / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014025:Titanium
D001672:Biocompatible Materials; D015921:Dental Implants; C400567:LAMC2 protein, human; D007797:Laminin; D014025:Titanium