Differential gene expression profiling of CD34+ CD133+ umbilical cord blood hematopoietic stem progenitor cells.
Umbilical cord blood (CB)-derived primitive hematopoietic stem progenitor cells (HSPC) are a promising source for stem cell-based gene therapy due to the reduced incidence and severity of graftversus- host disease (GVHD) after human leukocyte antigen (HLA)-disparate CB transplantation. Cell-surface markers such as CD34 and CD133 have been used in combination to enrich primitive HSPC for research and clinical applications. To understand the molecular characteristics of the CB HSPC, we compared the global gene expression of freshly isolated CB CD34+ CD133+ cells with their progenies using a cDNA microarray containing 22,000 human cDNA clones printed on a single chip. A total of 139 genes were differentially expressed between CB HSPC and their progenies. These transcripts included a number of known genes that might play roles in key functions of CB HSPC as well as many genes of unknown function. Among the genes showing the greatest differential expression levels in HSPC were: psoriasin 1, CRHBP, HDAC3, MLLT3, HBEX2, SPINK2, c-kit, H2BFQ, CD133, HHEX, TCF4, ALDH1A1, and FHL1. These data provide more information on the molecular phenotype of CB HSPC and may lead to the identification of new genes critical to stem cell function.
He
Xianghui
X
Gene Therapy Group, Department of Microbiology and Immunology, University of Arizona, Tucson, AZ 85724, USA.
Gonzalez
Veronica
V
Tsang
Amy
A
Thompson
Jennifer
J
Tsang
Tom C
TC
Harris
David T
DT
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000096:biosynthesis*; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000378:metabolism; D005231:Fatty Acids, Unsaturated; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis*; D006086:Graft vs Host Disease / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides; D010641:Phenotype; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D014470:Umbilical Cord / Q000166:cytology*
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C055649:9-(4-chlorophenyl) 7,7-dimethyl-5,8-nonadienoic acid methyl ester; D012313:RNA