Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.
Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.
Nagata
Reiko
R
Immune System Section, Department of Immunology, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan 305-0856. kikuma@affrc.go.jp
Muneta
Yoshihiro
Y
Yoshihara
Kazuhiro
K
Yokomizo
Yuichi
Y
Mori
Yasuyuki
Y
D020816:Amino Acid Motifs; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D002417:Cattle; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000096:biosynthesis*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology
D000942:Antigens, Bacterial; D011994:Recombinant Proteins; D007371:Interferon-gamma