Central leptin differentially modulates ultradian secretory patterns of insulin, leptin and ghrelin independent of effects on food intake and body weight.
We tested the hypothesis that leptin acts centrally to differentially modulate the ultradian communication of leptin, insulin and ghrelin with the hypothalamus. The ultradian fluctuation of these hormones in plasma after central leptin gene therapy was analyzed. Increased leptin transgene expression in the hypothalamus significantly decreased energy intake and body weight concomitant with severe hypoleptinemia and hypoinsulinemia resulting from drastically suppressed peak heights with unchanged frequency discharge of these hormones. Ghrelin secretion was, however, increased solely due to increased pulse amplitude. In pair-fed control rats leptin and ghrelin secretion was unchanged. In conclusion, independent of restraint on caloric intake and weight, leptin acting centrally modulates only the pulse amplitude of ultradian rhythmicity of the three afferent signals involved in the hypothalamic integration of energy balance. Since rhythmic discharge patterns dictate target response of hormones, these findings reveal a novel hypothalamic action of leptin in the pathophysiology of the obesity-dependent metabolic syndrome.
Otukonyong
Effiong E
EE
Department of Neuroscience, McKnight Brain Institute, University of Florida, College of Medicine, P.O. Box 100244, Gainesville, FL 32610-0244, USA.
Dube
Michael G
MG
Torto
Rita
R
Kalra
Pushpa S
PS
Kalra
Satya P
SP
D000256:Adenoviridae; D000818:Animals; D001835:Body Weight; D004435:Eating; D004734:Energy Metabolism; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D054439:Ghrelin; D007031:Hypothalamus / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000235:genetics; D008659:Metabolic Diseases / Q000097:blood / Q000150:complications; D009765:Obesity / Q000097:blood / Q000150:complications; D036361:Peptide Hormones / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019076:Transgenes
D054439:Ghrelin; D007328:Insulin; D020738:Leptin; D036361:Peptide Hormones