Glycolytic oscillations and waves in an open spatial reactor: Impact of feedback regulation of phosphofructokinase.
An open spatial reactor has been designed for the investigation of spatio-temporal dynamics of glycolysis. The reactor consists of a diffusive layer made of gel-fixed yeast extract which is in contact with a continuously stirred reservoir to supply this layer with substrates. The coupling between reaction and diffusion in the gel layer enables the formation of spatio-temporal patterns. Temporal oscillations of glycolysis are simply induced by feeding the yeast extract with sugar. Under properly chosen conditions, these oscillations sustain for more than 12 h. A necessary prerequisite for the generation of oscillations is that the ATP concentration in the feeding solution must be high enough to allow for negative feedback of phosphofructokinase. Otherwise, the interplay between ATP-consuming and ATP-producing reactions leads to an unfavorable low ATP/AMP ratio. The generation of travelling NADH-waves is observed in the diffusive layer, when feeding the yeast extract with substrates. Break-up of circular-shaped waves is repeatedly observed, resulting in the formation of rotating NADH-spirals.
Bagyan
Satenik
S
Institute of Experimental Physic, Group of Biophysics, Otto-von-Guericke-University of Magdeburg, Universitätsplatz 2, D-39106 Magdeburg, Germany.
Mair
Thomas
T
Dulos
Etiennette
E
Boissonade
Jacques
J
De Kepper
Patrick
P
Müller
Stefan C
SC
D000249:Adenosine Monophosphate / Q000378:metabolism / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D019149:Bioreactors; D002138:Calibration; D004058:Diffusion; D004563:Electrochemistry; D025461:Feedback, Physiological; D006019:Glycolysis / Q000187:drug effects; D008567:Membranes, Artificial; D009243:NAD / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D025221:Phosphofructokinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013050:Spectrometry, Fluorescence
D008567:Membranes, Artificial; D009243:NAD; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D025221:Phosphofructokinases