title: Crystallization of soluble urokinase receptor (suPAR) in complex with urokinase amino-terminal fragment (1-143).
abstract: Urokinase-type plasminogen activator (urokinase, uPA) and its receptor, uPAR, have been implicated in cell adhesion, migration, tissue remodelling and tumour-cell invasion. uPAR has three domains and is anchored to membranes by a glycosyl-phosphatidylinositol (GPI) anchor. Recombinant uPAR without its GPI anchor, soluble uPAR (suPAR), tends to oligomerize, making it difficult to crystallize. The amino-terminal fragment (ATF) of uPA is the major receptor-binding determinant in suPAR and binds to suPAR with nanomolar affinity, indistinguishable from membrane-bound uPAR. It is shown that uPA is capable of dissociating the oligomerization of suPAR and the crystallization of the suPAR-ATF complex is reported here. The resulting crystals diffract to 3.1 A using a synchrotron X-ray source.
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, People's Republic of China. mhuang@fjirsm.ac.cn
authors.forename: Mingdong
authors.initials: M
authors.lastname: Huang
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Mazar
authors.forename: Graham
authors.initials: G
authors.lastname: Parry
authors.forename: Abd Al-Roof
authors.initials: AA
authors.lastname: Higazi
authors.forename: Alice
authors.initials: A
authors.lastname: Kuo
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Cines
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry*; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry*
chemical_list: C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator