title: Lack of insulin-like growth factor binding protein-3 protease activation by venous cannulation.
abstract: Disruption of the endothelium activates thrombogenic and fibrinolytic enzymes that cleave insulin-like growth factor binding protein-3 (IGFBP-3) in vitro. The aim of the present human study was to determine whether blood sampling, i.e., venous stasis and cannulation increase IGFBP-3 proteolysis before and/or after surgery by activating these enzymes. Serum samples obtained immediately after cannulation were compared with samples obtained from a previously inserted venous catheter. Cannulation did not increase serum IGFBP-3 proteolytic activity pre- and post-operatively, as determined by in vitro degradation of 125I-IGFBP-3. Furthermore, there was no effect on in vivo IGFBP-3 fragmentation assessed by western immunoblot. In addition, a standardized venous stasis did not affect IGFBP-3 proteolytic activity or fragmentation. Comparison of IGFBP-3 proteolytic activity before and after surgery demonstrated a significant post-operative increase. However, this could not be demonstrated immediately after the initial cannulation, due to a large individual variation at this time-point before surgery.
authors.affiliation: Department of Woman and Child Health, Karolinska Institute, Pediatric Endocrinology Unit, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Gustafsson
authors.forename: C
authors.initials: C
authors.lastname: Carlsson-Skwirut
authors.forename: U
authors.initials: U
authors.lastname: Berg
authors.forename: J
authors.initials: J
authors.lastname: Nygren
authors.forename: P
authors.initials: P
authors.lastname: Bang
mesh_terms: D000005:Abdomen / Q000601:surgery; D002406:Catheterization, Peripheral; D010450:Endopeptidases / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood* / Q000378:metabolism; D008297:Male; D011184:Postoperative Period; D014680:Veins / Q000166:cytology
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D010450:Endopeptidases; C080473:insulin-like growth factor binding protein-3 protease