[Study on the correlation of transforming growth factor beta1 and its receptors with spontaneous abortion after in vitro fertilization and embryo transfer].
OBJECTIVE
To understand the expressions of transforming growth factor beta1 (TGF-beta1) and its receptors (TbetaR) in villi and decidua during early pregnancy and their effects on early embryo development, and explore the mechanism of spontaneous abortion.


METHODS
By immunohistochemical technique, expressions of TGF-beta1 and its receptors were determined in villi and decidua from 10 cases of spontaneous abortion women after in vitro fertilization and embryo transfer (IVF-ET group), 20 cases of spontaneous abortion women (spontaneous abortion group), and 20 cases of normal early pregnancy women (control group).


RESULTS
TGF-beta1 and its receptors had high expressions in decidua cells, villus glands and interstitial cytoplasm in all three groups. The average light density of the expressions of TGF-beta1 in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.167 and 0.199, 0.198 and 0.201, 0.277 and 0.274, respectively. The intensity of TbetaR-I in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.144 and 0.150, 0.202 and 0.201, 0.238 and 0.281, respectively. And the expressions of TbetaR-II in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.199 and 0.145, 0.153 and 0.156, 0.300 and 0.301, respectively. The differences between the control group and both abortion groups were all significant (P < 0.01).


CONCLUSIONS
The expressions of TGF-beta1 and its receptors in the early pregnancy tissues of aborted women declined. It demonstrates that the abnormal expressions of TGF-beta1 and its receptors play an important role in spontaneous abortion, they are one of the causes of spontaneous abortion.
Cheng
Ling-hui
LH
Department of Obstetrics and Gynecology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
Cao
Yun-xia
YX
D000022:Abortion, Spontaneous / Q000378:metabolism*; D000328:Adult; D002824:Chorionic Villi / Q000378:metabolism; D003656:Decidua / Q000378:metabolism; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism / Q000628:therapy; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II