Expression of PKC-beta or cyclin D2 predicts for inferior survival in diffuse large B-cell lymphoma.
We sought to determine whether identification of poor-risk subgroups of diffuse large B-cell lymphoma (DLBCL) using immunohistochemical stains would have practical utility with regard to prognosis and therapeutic decisions. Tissue microarray blocks were created using replicate samples of formalin-fixed, paraffin-embedded tissue from 200 cases of de novo DLBCL. The sections were stained with antibodies to proteins that are expressed by activated or proliferating B cells including MUM1, FOXP1, bcl-2, survivin, protein kinase C-beta (PKC-beta), cyclin D2, cyclin D3, and Ki-67. In univariate analysis, tumor expression of cyclin D2 (P = 0.025) or PKC-beta (P = 0.015) was associated with a worse overall survival, whereas none of the other markers was predictive of overall survival. Patients with DLBCL that expressed either cyclin D2 or PKC-beta had a 5-year overall survival of only 30% as compared to 52% for those who were negative for both markers (P = 0.0019). In multivariate analysis, the expression of cyclin D2 or PKC-beta was an independent predictor of poor overall survival (P = 0.035). Cyclin D2 and PKC-beta expression will be useful in designing a 'biological prognostic index' for patients with DLBCL.
Hans
Christine P
CP
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-3135, USA. chans@unmc.edu
Weisenburger
Dennis D
DD
Greiner
Timothy C
TC
Chan
Wing C
WC
Aoun
Patricia
P
Cochran
Gregory T
GT
Pan
Zenggang
Z
Smith
Lynette M
LM
Lynch
James C
JC
Bociek
Robert Gregory
RG
Bierman
Philip J
PJ
Vose
Julie M
JM
Armitage
James O
JO
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D056742:Cyclin D2; D016213:Cyclins / Q000096:biosynthesis*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000401:mortality*; D008875:Middle Aged; D011237:Predictive Value of Tests; D011493:Protein Kinase C / Q000096:biosynthesis*; D064546:Protein Kinase C beta; D015996:Survival Rate; D046888:Tissue Array Analysis
D014408:Biomarkers, Tumor; C080336:CCND2 protein, human; D056742:Cyclin D2; D016213:Cyclins; D011493:Protein Kinase C; D064546:Protein Kinase C beta