[Changes in functional state of the low-molecular G-proteins of platelets in type 1 diabetes mellitus].
Platelets from healthy donors and insuline dependent patients with type 1 diabetes mellitus were examined for proteins specifically interacting in vitro with GST-fused constitutively active (Val12) forms of small GTPases of Rac, Rho and Cdc42. Differential changes in pattern of proteins which bind to these G-proteins in diabetic platelets have been revealed. Obtained results suggest that signalling pathways mediated by Rho GTPases are altered under type I diabetes mellitus. Such changes of actin cytoskeleton regulation may contribute to the higher level of platelet aggregation, which prove to be the etiological background of the late diabetes complications.
Sybirna
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Vovk
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OI
Drobot
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LB
D000199:Actins / Q000378:metabolism; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010974:Platelet Aggregation; D011994:Recombinant Proteins / Q000378:metabolism
D000199:Actins; D020690:GTPase-Activating Proteins; D011994:Recombinant Proteins; C085379:rho GTPase-activating protein