title: [Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence].
abstract: OBJECTIVE
  To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence.
  
  
  METHODS
  Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining.
  
  
  RESULTS
  At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells.
  
  
  CONCLUSIONS
  Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China. hbsyyqsdr@163.com
authors.forename: Qi-sheng
authors.initials: QS
authors.lastname: Yao
authors.forename: Zhang-qun
authors.initials: ZQ
authors.lastname: Ye
authors.forename: Cong-bo
authors.initials: CB
authors.lastname: Chen
authors.forename: Xiao-kang
authors.initials: XK
authors.lastname: Wang
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Wang
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival; D017690:Cell Transplantation; D005260:Female; D018482:Muscle, Skeletal / Q000166:cytology*; D011183:Postoperative Complications / Q000628:therapy*; D011468:Prostatectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous; D014521:Urethra / Q000166:cytology; D001743:Urinary Bladder / Q000166:cytology; D014549:Urinary Incontinence / Q000209:etiology / Q000628:therapy*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001616:beta-Galactosidase