Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae.
The sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox herpesvirus (Cyprinid herpesvirus 1, CyHV-1), haematopoietic necrosis herpesvirus of goldfish (Cyprinid herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid herpesvirus 3 (CyHV-3).
Waltzek
Thomas B
TB
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Kelley
Garry O
GO
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Stone
David M
DM
The Centre for the Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
Way
Keith
K
The Centre for the Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
Hanson
Larry
L
Department of Basic Sciences, College of Veterinary Medicine, PO Box 6100 Mississippi State, MS 39762, USA.
Fukuda
Hideo
H
Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Hirono
Ikuo
I
Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Aoki
Takashi
T
Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Davison
Andrew J
AJ
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Hedrick
Ronald P
RP
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002347:Carps / Q000821:virology; D002397:Catfishes / Q000821:virology; D004265:DNA Helicases; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005393:Fish Diseases / Q000821:virology*; D016679:Genome, Viral; D006054:Goldfish / Q000821:virology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007913:Herpesvirus 1, Ranid; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity; D014758:Viral Core Proteins / Q000235:genetics
D036022:Capsid Proteins; D014758:Viral Core Proteins; C065639:DNA polymerase A; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases