title: Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated Douglas-Firby novel and commercial fungal cellulases.
abstract: Softwood residues are the most abundant feedstock available for bioconversion in many northern countries. However, the high costs for delignification and enzymatic hydrolysis currently deter commercialization of softwood bioconversion processes. This study evaluates the abilities of two novel fungal preparations (MSUBC1 and MSUBC2) and two commercial cellulase preparations (TR1 and TR2) to hydrolyze cellulose in Douglas-firpretreated by steam explosion or ethanol organosolv process. MSUBC1 showed significantly better performance than the other preparations on both lignocellulosic substrates. In particular, MSUBC1 achieved >76% cellulose conversion for hydrolysis of steam-exploded Douglas-fir (approximately 44% lignin) after 72 h at low enzyme loading (10 filter paper units/g of cellulose) and without beta-glucosidase supplementation.
authors.affiliation: Forest Products Biotechnology, Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.
authors.forename: Arwa
authors.initials: A
authors.lastname: Kurabi
authors.forename: Alex
authors.initials: A
authors.lastname: Berlin
authors.forename: Neil
authors.initials: N
authors.lastname: Gilkes
authors.forename: Douglas
authors.initials: D
authors.lastname: Kilburn
authors.forename: Renata
authors.initials: R
authors.lastname: Bura
authors.forename: Jamie
authors.initials: J
authors.lastname: Robinson
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Markov
authors.forename: Anton
authors.initials: A
authors.lastname: Skomarovsky
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Gusakov
authors.forename: Oleg
authors.initials: O
authors.lastname: Okunev
authors.forename: Arkady
authors.initials: A
authors.lastname: Sinitsyn
authors.forename: David
authors.initials: D
authors.lastname: Gregg
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: John
authors.initials: J
authors.lastname: Saddler
mesh_terms: D001673:Biodegradation, Environmental; D044602:Cellulases / Q000737:chemistry*; D004789:Enzyme Activation; D000431:Ethanol / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D010407:Penicillium / Q000201:enzymology*; D028224:Pseudotsuga / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology*; D014867:Water / Q000737:chemistry*; D014934:Wood
chemical_list: D014867:Water; D000431:Ethanol; D008031:Lignin; D044602:Cellulases