title: Rational strategy for the production of new crude lipases from Candida rugosa.
abstract: Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis.
authors.affiliation: Grupo de Biotransformaciones, Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad Complutense Madrid, Plaza de Ramón y Cajal, s/n. Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Pablo Domínguez
authors.initials: PD
authors.lastname: de María
authors.forename: José M
authors.initials: JM
authors.lastname: Sánchez-Montero
authors.forename: Andrés R
authors.initials: AR
authors.lastname: Alcántara
authors.forename: Francisco
authors.initials: F
authors.lastname: Valero
authors.forename: José V
authors.initials: JV
authors.lastname: Sinisterra
mesh_terms: D002175:Candida / Q000201:enzymology*; D007851:Dodecanol / Q000378:metabolism; D004790:Enzyme Induction; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D000074242:Sunflower Oil
chemical_list: D007527:Isoenzymes; D009829:Oleic Acids; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D007851:Dodecanol; D019301:Oleic Acid; C068800:heptyl oleate; D008049:Lipase; D005990:Glycerol