Recovery of catechin compounds from Korean tea by solvent extraction.
Catechin compounds from Korean green tea as potential sources of anticancer and antioxidant components were target materials in this work. The methodologies of solvent extraction and partition were utilized to recover catechin compounds from green tea. The optimum experimental condition was obtained by optimizing operating factors, such as, the extraction solvent, extraction time and operating temperature. After extracting the green tea with water at 80 degrees C for 40 min, the extract was partitioned with water/chloroform, which was best suited to remove caffeine impurity from the extract. Further, the resulting extract was partitioned water/ethyl acetate to deeply purify the catechin compounds of EGC, EC, EGCG and ECG. The experimental result in this work could be extended to preparative HPLC to obtain EGCG on commercial scale.
Row
Kyung Ho
KH
Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Korea. rowkho@inha.ac.kr
Jin
Yinzhe
Y
D000085:Acetates; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000302:isolation & purification*; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D007723:Korea; D010936:Plant Extracts / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D014867:Water
D000085:Acetates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; C007650:ethyl acetate; D002725:Chloroform; D002392:Catechin