Contribution of glutamate receptors to brain-derived neurotrophic factor-induced elevation of intracellular Ca2+levels.
In the present study, acute effects of brain-derived neurotrophic factor (BDNF) on intracellular calcium concentration ([Ca2+]i) in cultured visual cortical neurons were investigated. We examined specifically whether pharmacological blockade of glutamatergic receptors interfered with effects of BDNF on [Ca2+]i. We found that blockade of N-methyl-D-aspartate (NMDA) receptors significantly reduced the number of BDNF-responsive neurons, while inhibition of metabotropic glutamate receptors (mGluR) completely prevented the effect of BDNF. By contrast, blockade of alpha-amino-3-hydroxy-5-methyl-4-isoxasole propionic acid (AMPA) receptors did not affect the BDNF-induced increase of [Ca2+]i. Our results thus suggest that glutamate-mediated excitatory pathways are involved in the BDNF-induced rise of [Ca2+]i in visual cortical neurons, and that both mGluR and NMDA receptors play a critical role in this event.
Yang
Benduan
B
Brain Research Center and Department of Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada.
Gu
Qiang
Q
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004622:Embryo, Mammalian; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D005998:Glycine / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017470:Receptors, Glutamate / Q000502:physiology*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014793:Visual Cortex / Q000166:cytology*
D019208:Brain-Derived Neurotrophic Factor; D018691:Excitatory Amino Acid Antagonists; D017470:Receptors, Glutamate; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D015763:2-Amino-5-phosphonovalerate; D002118:Calcium; D005998:Glycine