Gene transfer of glutamic acid decarboxylase reduces neuropathic pain.
We tested whether transfer of the gene coding for glutamic acid decarboxylase to dorsal root ganglion using a herpes simplex virus vector to achieve release of GABA in dorsal horn would attenuate nociception in this condition. Subcutaneous inoculation of a replication-defective herpes simplex virus vector expressing glutamic acid decarboxylase (vector QHGAD67) 7 days after selective L5 spinal nerve ligation reversed mechanical allodynia and thermal hyperalgesia; the antiallodynic effect lasted 6 weeks and was reestablished by reinoculation. QHGAD67 inoculation also suppressed induction of c-Fos and phosphorylated extracellular signal-regulated kinase 1 and 2 in the spinal cord.
Hao
Shuanglin
S
Department of Neurology, University of Michigan Health System and Ann Arbor VA Healthcare System, Ann Arbor, MI 48109-0316, USA.
Mata
Marina
M
Wolfe
Darren
D
Huang
Shaohua
S
Glorioso
Joseph C
JC
Fink
David J
DJ
D000818:Animals; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D006930:Hyperalgesia / Q000628:therapy; D008026:Ligation; D009437:Neuralgia / Q000628:therapy*; D020671:Posterior Horn Cells / Q000502:physiology; D051381:Rats; D018139:Simplexvirus / Q000235:genetics; D013127:Spinal Nerves; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase