title: Sublingual specific immunotherapy reduces PBMC proliferations.
abstract: BACKGROUND
  Subcutaneous specific immunotherapy has been demonstrated capable of inducing T regulatory response. There is few evidence concerning immunological changes induced by sublingual immunotherapy.
  
  
  OBJECTIVE
  The aim of this study was to evaluate T cell proliferation in subjects successfully treated with SLIT for HDM.
  
  
  METHODS
  PBMCs were isolated from patients after at least 3 years of successful HDM SLIT and from matched untreated allergic and healthy control subjects. After 3 and 6 days of in vitro stimulation with PHA, Candida albicans, Dermatophagoides farinae, grasses, Parietaria judaica, and cat, proliferation.
  
  
  RESULTS
  Subjects treated with SLIT showed significant reduction of proliferation induced by Candida albicans, Parietaria, and grasses in comparison with untreated atopics (p=0.0002, 0.0033, and 0.009 respectively).
  
  
  CONCLUSIONS
  This pilot study confirms reduced T cell proliferation in allergic subjects treated with SLIT.
authors.affiliation: DIMI-CEBR, Università di Genova, Genoa, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Fenoglio
authors.forename: F
authors.initials: F
authors.lastname: Puppo
authors.forename: I
authors.initials: I
authors.lastname: Cirillo
authors.forename: A
authors.initials: A
authors.lastname: Vizzaccaro
authors.forename: A
authors.initials: A
authors.lastname: Ferrera
authors.forename: M A
authors.initials: MA
authors.lastname: Tosca
authors.forename: G
authors.initials: G
authors.lastname: Marseglia
authors.forename: G
authors.initials: G
authors.lastname: Ciprandi
mesh_terms: D000286:Administration, Sublingual; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use; D002176:Candida albicans / Q000276:immunology; D002415:Cats / Q000276:immunology; D040001:Dermatophagoides farinae / Q000276:immunology; D003888:Desensitization, Immunologic; D005260:Female; D006197:Hair / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D032403:Parietaria / Q000276:immunology; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D012882:Skin Tests; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides