title: Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture.
abstract: Basigin, an IgG family glycoprotein found on the surface of human metastatic tumors, stimulates fibroblasts to secrete matrix metalloproteases that remodel the extracellular matrix. Using Drosophila melanogaster we identify intracellular, matrix metalloprotease-independent, roles for basigin. Specifically, we found that basigin, interacting with integrin, is required for normal cell architecture in some cell types. Basigin promotes cytoskeletal rearrangements and the formation of lamellipodia in cultured insect cells. Loss of basigin from photoreceptors leads to misplaced nuclei, rough ER and mitochondria, as well as to swollen axon terminals. These changes in intracellular structure suggest cytoskeletal disruptions. These defects can be rescued by either fly or mouse basigin. Basigin and integrin colocalize to cultured cells and to the visual system. Basigin-mediated changes in the architecture of cultured cells require integrin binding activity. Basigin and integrin interact genetically to affect cell structure in the animal, possibly by forming complexes at cell contacts that help organize internal cell structure.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA. kcurtin@uark.edu
authors.forename: Kathryn D
authors.initials: KD
authors.lastname: Curtin
authors.forename: Ian A
authors.initials: IA
authors.lastname: Meinertzhagen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wyman
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051926:Basigin; D048430:Cell Shape; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D012160:Retina / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D017385:Sequence Homology
chemical_list: D000199:Actins; D015703:Antigens, CD; C497886:BSG protein, human; C504909:Bsg protein, Drosophila; C497887:Bsg protein, mouse; D029721:Drosophila Proteins; D016023:Integrins; D008562:Membrane Glycoproteins; D051926:Basigin; D020778:Matrix Metalloproteinase 2