title: Robust hepatitis C virus infection in vitro.
abstract: The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.
authors.affiliation: Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhong
authors.forename: Pablo
authors.initials: P
authors.lastname: Gastaminza
authors.forename: Guofeng
authors.initials: G
authors.lastname: Cheng
authors.forename: Sharookh
authors.initials: S
authors.lastname: Kapadia
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kato
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.forename: Stefan F
authors.initials: SF
authors.lastname: Wieland
authors.forename: Susan L
authors.initials: SL
authors.lastname: Uprichard
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Francis V
authors.initials: FV
authors.lastname: Chisari
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D018922:HL-60 Cells; D006367:HeLa Cells; D016174:Hepacivirus / Q000378:metabolism*; D006526:Hepatitis C / Q000473:pathology*; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D012313:RNA / Q000737:chemistry; D013395:Sucrose / Q000494:pharmacology; D060225:Tetraspanin 28; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D020298:U937 Cells
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; D018076:DNA, Complementary; D060225:Tetraspanin 28; D013395:Sucrose; D012313:RNA