Capillary electrophoretic determination of triamterene, methotrexate, and creatinine in human urine.
A capillary zone electrophoresis (CZE) method using a fused-silica capillary (60.2 cm x 75 microm ID) was investigated for the determination of triamterene (TRI), methotrexate (MTX), and creatinine (CREA) in human urine. The separation was performed using a hydrodynamic injection time of 7 s (0.5 psi), a voltage of 25 kV, a capillary temperature of 30 degrees C, and 40 mM phosphoric acid adjusted to pH 2.25 by addition of triethanolamine as separation electrolyte. Under these conditions, analysis takes about 15 min. A linear response over the 0.5-15.0 mg L(-1) concentration range was found for TRI and MTX, and 0.5-80.0 mg L(-1) for CREA. Dilution of the sample (water:urine, 1:1 for TRI and MTX, and 1:25 for CREA determination) was the only step necessary prior to analysis by electrophoresis. The developed method is easy, rapid, and sensitive and has been applied to determine triamterene,methotrexate, and creatinine in urine samples with satisfactory results.
Rodríguez Flores
Juana
J
Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, 13071 Ciudad Real, Spain. juana.rflores@uclm.es
Berzas Nevado
Juan J
JJ
Durán Merás
Isabel
I
Rodríguez Gómez
Maria J
MJ
D000328:Adult; D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine*; D004232:Diuretics / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008727:Methotrexate / Q000652:urine*; D015394:Molecular Structure; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014223:Triamterene / Q000652:urine*
D000964:Antimetabolites, Antineoplastic; D004232:Diuretics; D003404:Creatinine; D014223:Triamterene; D008727:Methotrexate