title: Aldose reductase inhibitors from the fruiting bodies of Ganoderma applanatum.
abstract: The isolation and characterization of rat lens aldose reductase (RLAR) inhibitors from the fruiting bodies of Ganoderma applanatum were conducted. Among the extracts and fractions from G. applanatum tested, the MeOH extract and EtOAc fraction were found to exhibit potent RLAR inhibition in vitro, their IC50 being 1.7 and 0.8 microg/ml, respectively. From the active EtOAc fraction, seven compounds with diverse structural moieties were isolated and identified as D-mannitol (1), 2-methoxyfatty acids (2), cerebrosides (3), daucosterol (4), 2,5-dihydroxyacetophenone (5), 2,5-dihydroxybenzoic acid (6), and protocatechualdehyde (7). Among them, protocatechualdehyde (7) was found to be the most potent RLAR inhibitor (IC50=0.7 microg/ml), and may be useful for the prevention and/or treatment of diabetic complications.
authors.affiliation: Natural Products Research Institute and College of Pharmacy, Seoul National University, Japan.
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
authors.forename: Ju Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Kuk Hyun
authors.initials: KH
authors.lastname: Shin
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000302:isolation & purification; D032902:Ganoderma; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004791:Enzyme Inhibitors; D000449:Aldehyde Reductase