Developmental expression of sema3G, a novel zebrafish semaphorin.
The semaphorins are a large, evolutionarily conserved family of signaling molecules with broad functions during development. The class 3 semaphorins are a subclass of secreted semaphorins found in vertebrates. There have been six class 3 semaphorins identified to date (sema3A to sema3F) and some have been shown to function in axon guidance and cardiovascular development. However, the functions of many class 3 semaphorins and their potential interactions in vivo are still not well understood. As a step toward understanding the actions of all class 3 semaphorins in vivo, we have cloned and analyzed the developmental expression pattern of a novel zebrafish class 3 semaphorin, sema3H [corrected] sema3H [corrected] is expressed in a dynamic pattern throughout the first 3 days of development. It is expressed in the adaxial cells of the somite during somitogenesis. In the brain, sema3H [corrected] is expressed in cell clusters in the midbrain and diencephalon, and is expressed in the telencephalon in close proximity to the olfactory epithelium. sema3H [corrected] also is expressed in the pharyngeal arches, the pectoral fin bud, and the developing pronephros. These results provide a basis for studying how expression of multiple semaphorins could be essential for aspects of early development.
Stevens
Craig B
CB
Department of Zoology, University of Wisconsin, Madison, 53706, USA.
Halloran
Mary C
MC
D000818:Animals; D001369:Axons / Q000378:metabolism; D001921:Brain / Q000196:embryology; D002319:Cardiovascular System; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D007136:Immunoglobulins / Q000378:metabolism; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics*; D009900:Optic Nerve / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D039961:Semaphorins / Q000096:biosynthesis* / Q000235:genetics*; D019170:Somites; D013687:Telencephalon / Q000378:metabolism; D013997:Time Factors; D015027:Zebrafish
D007136:Immunoglobulins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D039961:Semaphorins; C115735:semaphorin Z8