Separation of compounds interacting with liposome membrane in combined prescription of traditional Chinese medicines with immobilized liposome chromatography.
Immobilized liposome chromatography (ILC), the stationary phase of which has been regarded as a mimic biomembranes system was used to separate and analyze compounds interacting with liposome membrane in Danggui Buxue decoction, a combined prescription of traditional Chinese medicines (CPTCMs), and its compositions Radix Astragli and Radix Angelica Sinensis. More than 10 main peaks in the extract of Danggui Buxue decoction were resolved on the ILC column, suggesting that more than 10 components in the prescription have significant retention on ILC column. Ligustilide, astragaloside IV and formononetin, three main bioactive ingredients in Danggui Buxue decoction, were found to have relatively significant, while ferulic acid, another bioactive ingredient in the prescription, relatively weak retention on ILC column. Effects of the eluent pH and amount of immobilized phosphatidylcholine (PC) on separation of interactional compounds in the extract of Danggui Buxue decoction were also investigated. It was found that these two factors strongly affected the retention of some interactional compounds. In addition, the fractions partitioned with different solvents from water extract of this combined prescription were evaluated with this ILC column system.
Sheng
Liang-Hong
LH
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116011, China. sh_lianghong@163.com
Li
Song-Lin
SL
Kong
Liang
L
Chen
Xue-Guo
XG
Mao
Xi-Qin
XQ
Su
Xing-Ye
XY
Zou
Han-Fa
HF
Li
Ping
P
D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000032:analysis; D008081:Liposomes / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D012503:Saponins / Q000032:analysis; D013678:Technology, Pharmaceutical / Q000379:methods; D014315:Triterpenes / Q000032:analysis
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D008081:Liposomes; D010713:Phosphatidylcholines; D012503:Saponins; D014315:Triterpenes; C477279:danggui buxue decoction; C007768:formononetin; C052064:astragaloside A; C004999:ferulic acid