Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent.
The development of a process intensified primary capture step for the direct selective recovery of intracellular proteins from very dense particulate-containing yeast extract has been explored. The purification of glyceraldehyde 3-phosphate dehydrogenase from bakers' yeast was chosen as a potential demonstration of this approach. A high throughput (50%, w/v, yeast extracts at a superficial linear velocity of 450 cm h(-1)) was achieved by adoption of a high-density adsorbent (UpFront steel-agarose; rho = 2.65 g ml(-1)) derivatized with selective ligand chemistries (Cibacron Blue 3GA). This should ultimately minimize adsorption time and maximize process efficiency of fluidized bed adsorption.
Ling
Tau Chuan
TC
Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering, University of Birmingham, Edgbaston, UK. ltc555@eng.upm.edu.my
Lyddiatt
Andrew
A
D000465:Algorithms; D018533:Biomass; D002846:Chromatography, Affinity / Q000379:methods; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000302:isolation & purification*; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D013232:Steel / Q000737:chemistry
D029701:Saccharomyces cerevisiae Proteins; C017842:cibacron blue-sepharose; D013232:Steel; D012685:Sepharose; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)