OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation.
The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified. The expression of OsLSD1 was light-induced or dark-suppressed. Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants. Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1 mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus. Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus. Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin. OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells. Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.
Wang
Lijuan
L
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P R. China.
Pei
Zhongyou
Z
Tian
Yingchuan
Y
He
Chaozu
C
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D002734:Chlorophyll / Q000096:biosynthesis; D037341:Fumonisins / Q000633:toxicity; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology*; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D016335:Zinc Fingers / Q000502:physiology
D037341:Fumonisins; D010940:Plant Proteins; D002734:Chlorophyll; C056933:fumonisin B1; C037184:chlorophyll b