Effects of monocular enucleation on calbindin-D 28k and c-Fos expression in the lateral geniculate nucleus in rats.
The present study was undertaken to evaluate the effects of monocular enucleation on the calbindin-D 28k (CB) and c-Fos immunoreactive (IR) neurons in the lateral geniculate nucleus (LGN) complex of adult rats. The enucleation resulted in neuronal degeneration and decrease of neurons in the LGN complex. Our study demonstrated a decrease of CB-IR neuronal density on the contralateral side of the ventral (vLGN) and dorsal LGN (dLGN) until 12 weeks post-enucleation (PE). On the ipsilateral side, CB-IR neuronal density in the dLGN and vLGN showed significant and continuous decrease until 48 and 12 weeks PE, respectively. In an additional experiment, c-Fos-IR neurons were increased at 2 days PE in the vLGN with ipsilateral predominance. At 7 days, c-Fos-IR neurons on the ipsilateral vLGN were still higher than those of pre-enucleated rats. The present results suggest that monocular enucleation affects the expression of the CB and c-Fos in the LGN complex. It is indicated that these may play an important role in the neuronal degeneration and neuroplasticity of the subvisual system in rats.
Gonzalez
Daniel
D
Department of Anatomy and Neurobiology, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
Satriotomo
Irawan
I
Miki
Takanori
T
Lee
Kyoung-Youl
KY
Yokoyama
Toshifumi
T
Touge
Tetsuo
T
Matsumoto
Yoshiki
Y
Li
Hong-Peng
HP
Kuriyama
Shigeki
S
Takeuchi
Yoshiki
Y
D000818:Animals; D001766:Blindness / Q000378:metabolism* / Q000503:physiopathology; D064026:Calbindins; D002452:Cell Count; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D015353:Eye Enucleation; D007839:Functional Laterality / Q000502:physiology; D005829:Geniculate Bodies / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000503:physiopathology; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology; D012160:Retina / Q000293:injuries / Q000503:physiopathology; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
D064026:Calbindins; D016760:Proto-Oncogene Proteins c-fos; D064030:S100 Calcium Binding Protein G