Deletion of the ferric uptake regulator Fur impairs the in vitro growth and virulence of Actinobacillus pleuropneumoniae.
In order to investigate the role of the ferric uptake regulator Fur in the porcine lung pathogen Actinobacillus pleuropneumoniae, we constructed an isogenic in-frame deletion mutant, A. pleuropneumoniae Deltafur. This mutant showed constitutive expression of transferrin-binding proteins, growth deficiencies in vitro, and reduced virulence in an aerosol infection model.
Jacobsen
Ilse
I
Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Gerstenberger
Jörg
J
Gruber
Achim D
AD
Bossé
Janine T
JT
Langford
Paul R
PR
Hennig-Pauka
Isabel
I
Meens
Jochen
J
Gerlach
Gerald-F
GF
D000189:Actinobacillus Infections / Q000378:metabolism / Q000473:pathology; D016977:Actinobacillus pleuropneumoniae / Q000254:growth & development* / Q000472:pathogenicity; D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D007501:Iron / Q000378:metabolism; D008168:Lung / Q000473:pathology; D012097:Repressor Proteins / Q000502:physiology*; D013552:Swine; D033882:Transferrin-Binding Protein B / Q000235:genetics; D014774:Virulence
D001426:Bacterial Proteins; D012097:Repressor Proteins; D033882:Transferrin-Binding Protein B; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron