title: Intragenomic spliced leader RNA array analysis of kinetoplastids reveals unexpected transcribed region diversity in Trypanosoma cruzi.
abstract: The spliced leader RNA gene (SL RNA) repeat is present in large multicopy arrays and has been used as a marker for the diversity of kinetoplastid protozoans. Intra-array variation could affect conclusions made using a randomly isolated repeat as a marker. We examined the Leishmania major (Friedlin) and Trypanosoma cruzi (CL Brener) genome projects for SL RNA repeat sequences in order to assess their homogeneity and the possible effects of sequence variation on taxonomic interpretation. Of the dozens of distinct sequence classes examined, no single copy would bias clustering analyses with regard to other closely related species or isolates. Six dimorphic sites within the T. cruzi transcribed region were found to be linked and are predicted to yield a heterogeneous SL RNA population. The variation that exists among the repeats paints a picture of the broad mechanisms of array maintenance and evolution where site-specific mutations in a single repeat may be spread throughout the array and recombined with existing repeats to create new sequence classes, all occurring under selective pressure to maintain or increase the fitness of the cell line in which these events occur.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles, CA, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Thomas
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Westenberger
authors.forename: David A
authors.initials: DA
authors.lastname: Campbell
authors.forename: Nancy R
authors.initials: NR
authors.lastname: Sturm
mesh_terms: D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D014644:Genetic Variation; D018503:Genome, Protozoan; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D020038:RNA, Spliced Leader / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D020038:RNA, Spliced Leader