Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled receptor.
The seven-transmembrane-spanning G protein-coupled receptor (GPCR) superfamily plays many important roles in basic biology, human health, and human disease. Here, well-resolved solution NMR spectra are presented for a human GPCR, the vasopressin V2 receptor in detergent micelles. The quality of the NMR spectra indicates that backbone resonance assignments for a majority of resonances are feasible. The key to obtaining high quality spectra appears to be the coupling of methods for expressing the receptor into membranes rather than into inclusion bodies, with use of a biochemically mild lysolipid detergent for membrane extraction, protein purification, and NMR sample preparation.
Tian
Changlin
C
Center for Structural Biology and Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, USA.
Breyer
Richard M
RM
Kim
Hak Jun
HJ
Karra
Murthy D
MD
Friedman
David B
DB
Karpay
Anne
A
Sanders
Charles R
CR
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D017483:Receptors, Vasopressin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012996:Solutions
D043562:Receptors, G-Protein-Coupled; D017483:Receptors, Vasopressin; D011994:Recombinant Proteins; D012996:Solutions