title: Effect of hypercholesterolemia on transendothelial EBD-albumin permeability and lipid accumulation in porcine iliac arteries.
abstract: Hypercholesterolemia is associated with increased cardiovascular mortality and is known to promote the advancement of atherosclerotic lesions in experimental animal models. Juvenile swine were fed a normal or high-cholesterol diet, and the transendothelial macromolecular permeability of the external iliac arteries of these animals was assessed by measuring the uptake rate of circulating Evans blue dye (EBD). The extent and patterns of lipid-containing lesions were also determined using en face staining with Oil Red O (ORO). Sites of ORO staining often excluded EBD, possibly via the fragmentation of the internal elastic lamina, to which EBD binds. By spatially averaging the EBD uptake in arterial segments relatively free of ORO-positive lesions, it was found that endothelial permeability to albumin was greater in hypercholesterolemic pigs than in those on a normal diet (p=0.056).
authors.affiliation: Duke University, Department of Biomedical Engineering, P.O. Box 90281, Durham, NC 27708, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Lamack
authors.forename: Heather A
authors.initials: HA
authors.lastname: Himburg
authors.forename: Morton H
authors.initials: MH
authors.lastname: Friedman
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D001693:Biological Transport, Active / Q000502:physiology; D002199:Capillary Permeability; D004396:Coloring Agents / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D018450:Disease Progression; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005070:Evans Blue / Q000493:pharmacokinetics*; D005260:Female; D006937:Hypercholesterolemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D007083:Iliac Artery / Q000378:metabolism* / Q000473:pathology; D013552:Swine
chemical_list: D000418:Albumins; D004396:Coloring Agents; D005070:Evans Blue