title: Antitubercular activity and inhibitory effect on Epstein-Barr virus activation of sterols and polyisoprenepolyols from an edible mushroom, Hypsizigus marmoreus.
abstract: Seven sterols (1-7) and eight polyisoprenepolyols (8-15), isolated from the non-saponifiable lipid fraction of the dichloromethane extract of an edible mushroom, Hypsizigus marmoreus (Buna-shimeji), were tested for their antitubercular activity against Mycobacterium tuberculosis strain H37Rv using the Microplate Alamar Blue Assay (MABA). Six sterols (2-7) and two polyisoprenepolyols (8, 12) showed a minimum inhibitory concentration (MIC) in the range of 1-51 microg/ml, while the others (1, 9-11, 13-15) were inactive (MIC>128 microg/ml). The seven sterols (1-7) and three polyisoprenepolyols (8, 10, 12) were further evaluated for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells. Sterols 6 and 7 showed potent inhibitory effects while preserving the high viability of Raji cells.
authors.affiliation: College of Science and Technology, Nihon University, Tokyo, Japan. akihisa@chem.cst.nihon-u.ac.jp
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akihisa
authors.forename: Scott Gary
authors.initials: SG
authors.lastname: Franzblau
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tagata
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ukiya
authors.forename: Tsunetomo
authors.initials: T
authors.lastname: Matsuzawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Metori
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Ken
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D000363:Agaricales; D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002070:Butadienes / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D045782:Hemiterpenes / Q000302:isolation & purification / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010420:Pentanes / Q000302:isolation & purification / Q000494:pharmacology; D013261:Sterols / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D000998:Antiviral Agents; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D013261:Sterols; C005059:isoprene