Fluorescein-labeled sinistrin as marker of glomerular filtration rate.
There is an obvious and growing medical need for an accurate and easy to handle determination of glomerular filtration rate (GFR) for a broad spectrum of indications. Newly synthesized fluorescein-isothiocyanate (FITC)-sinistrin (FS) with various degrees of labeling was selected by its physicochemical properties and good tolerability out of a number of dye-labeled compounds intended for use as GFR markers for characterization of its pharmacological profile. With respect to solubility FS is more convenient in handling compared to FITC-inulin (FI). Up to 100 mg ml(-1) of FS can be dissolved in aqueous solvents at room temperature, whereas FI can only be solubilized after warming up to 55 degrees C. This reveals a considerable advantage of FS over FI in preparation of galenical formulations for intended i.v. application. A fluorometric method was established to determine FS concentration in blood serum with a comparable accuracy to the established enzymatic method for polyfructosanes. Similar concentration time curves in blood serum of FS measured fluorometrically and enzymatically suggest no relevant change of pharmacokinetic behavior by dye labeling. This notion is supported by the rapid renal and missing of biliary excretion. On the basis of these results, FS is superior in handling to the available GFR markers and makes it highly interesting as a novel diagnostic drug.
Pill
Johannes
J
Roche Diagnostics GmbH, Sandhofer Str. 116, D-68305 Mannheim, Germany. johannes.pill@roche.com
Kraenzlin
Bettina
B
Jander
Julia
J
Sattelkau
Tim
T
Sadick
Maliha
M
Kloetzer
Hans-Martin
HM
Deus
Carsten
C
Kraemer
Uwe
U
Gretz
Norbert
N
D000818:Animals; D015415:Biomarkers / Q000652:urine; D019793:Fluorescein / Q000032:analysis* / Q000737:chemistry*; D005919:Glomerular Filtration Rate / Q000502:physiology*; D008297:Male; D015394:Molecular Structure; D009844:Oligosaccharides / Q000009:adverse effects / Q000737:chemistry* / Q000493:pharmacokinetics / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D009844:Oligosaccharides; D019793:Fluorescein; C064636:sinistrin