title: Review of translocations detected by FISH for retrospective biological dosimetry applications.
abstract: Several European laboratories have combined their research efforts to arrive at a consensus view on using fluorescence in situ hybridisation (FISH) for retrospective dosimetry. The aim of this review is to report these views and to highlight some areas where further work is needed. Translocations in the stable cells should be measured only in the cells that contain the full complement of the painted material. Two-way and one-way translocations should be combined with equal weight. The control level of translocations has a strong dependence on age, which has now been measured and the system has been calibrated. In conclusion, the technique works and a lifetime dose to the bone marrow from low-linear energy transfer radiation of 0.5 Gy above normal background levels can be measured for any individual. The main application is considered to provide an independent verification of lifetime doses to individuals who might form a part of an epidemiological study.
authors.affiliation: Health Protection Agency, Radiation Protection Division (HPA), Chilton, Didcot, Oxon OX11 0RQ, UK. alan.edwards@hpa-rp.org.uk
authors.forename: A A
authors.initials: AA
authors.lastname: Edwards
authors.forename: C
authors.initials: C
authors.lastname: Lindholm
authors.forename: F
authors.initials: F
authors.lastname: Darroudi
authors.forename: G
authors.initials: G
authors.lastname: Stephan
authors.forename: H
authors.initials: H
authors.lastname: Romm
authors.forename: J
authors.initials: J
authors.lastname: Barquinero
authors.forename: L
authors.initials: L
authors.lastname: Barrios
authors.forename: M R
authors.initials: MR
authors.lastname: Caballin
authors.forename: L
authors.initials: L
authors.lastname: Roy
authors.forename: C A
authors.initials: CA
authors.lastname: Whitehouse
authors.forename: E J
authors.initials: EJ
authors.lastname: Tawn
authors.forename: J
authors.initials: J
authors.lastname: Moquet
authors.forename: D C
authors.initials: DC
authors.lastname: Lloyd
authors.forename: P
authors.initials: P
authors.lastname: Voisin
mesh_terms: D002138:Calibration; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016273:Occupational Exposure; D011834:Radiation Monitoring / Q000379:methods; D011874:Radiometry / Q000379:methods*; D014178:Translocation, Genetic