Preparation of uniform sized chitosan microspheres by membrane emulsification technique and application as a carrier of protein drug.
The control of size and size distribution of microspheres is necessary for obtaining repeatable controlled release behavior. The chitosan microspheres were prepared by a membrane emulsification technique in this study. Chitosan was dissolved in 1 wt.% aqueous acetic acid containing 0.9 wt.% sodium chloride, which was used as a water phase. A mixture of liquid paraffin and petroleum ether 7:5 (v/v) containing PO-500 emulsifier was used as an oil phase. The water phase was permeated through the uniform pores of a porous glass membrane into the oil phase by the pressure of nitrogen gas to form W/O emulsion. Then GST (Glutaraldehyde Saturated Toluene) as crosslinking agent was slowly dropped into the W/O emulsion to solidify the chitosan droplets. The preparation condition for obtaining uniform-sized microspheres was optimized. The microspheres with different size were prepared by using the membranes with different pore size, and there was a linear relationship between the diameter of microspheres and pore size of the membranes when the microspheres were in the range of micron size. The smallest chitosan microspheres obtained was 0.4 mum in diameter. This is the first report for preparing the uniform-sized chitosan microspheres by membrane emulsification technique. Uniform chitosan microspheres were further used as a carrier of protein drug. Bovine serum albumin (BSA) as a model drug was loaded in the microspheres and released in vitro. The effects of pH value, diameter and crosslinking degree of microspheres, and BSA concentration on loading efficiency and release behavior were discussed.
Wang
Lian-Yan
LY
State Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
Ma
Guang-Hui
GH
Su
Zhi-Guo
ZG
D000327:Adsorption; D000473:Alkanes / Q000737:chemistry; D000537:Aluminum Oxide / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D003369:Cottonseed Oil / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods*; D045703:Emulsifying Agents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008899:Mineral Oil / Q000737:chemistry; D000069463:Olive Oil; D010316:Particle Size; D010938:Plant Oils / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000473:Alkanes; D003369:Cottonseed Oil; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D045703:Emulsifying Agents; D000069463:Olive Oil; D010938:Plant Oils; D014867:Water; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D008899:Mineral Oil; D048271:Chitosan; D000537:Aluminum Oxide; C004544:naphtha; D005976:Glutaral