Biomolecule detection via target mediated nanoparticle aggregation and dielectrophoretic impedance measurement.
A new biosensing system is described that is based on the aggregation of nanoparticles by a target biological molecule and dielectrophoretic impedance measurement of these aggregates. The aggregation process was verified within a microchannel via fluorescence microscopy, demonstrating that this process can be used in a real time sensor application. Positive dielectrophoresis is employed to capture the nanoparticle aggregates at the edge of thin film electrodes, where their presence is detected either by optical imaging via fluorescence microscopy or by measuring the change in electrical impedance between adjacent electrodes. The electrical detection mechanism demonstrates the potential for this method as a micro total analysis system (microTAS).
Costanzo
Philip J
PJ
Chemistry Department, University of California at Davis, 95616, USA.
Liang
Enzhu
E
Patten
Timothy E
TE
Collins
Scott D
SD
Smith
Rosemary L
RL
D001360:Avidin / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019921:Biotinylation; D019187:Cadmium Compounds / Q000737:chemistry; D017097:Electric Impedance; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry*; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013499:Surface Properties
D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D001360:Avidin; D011092:Polyethylene Glycols; D012822:Silicon Dioxide