Measuring bacterial growth by tapered fiber and changes in evanescent field.
Single mode continuous tapered fibers were fabricated with waist diameters of 6-8 microm and of 11 mm waist lengths. The tapered surface was coated with poly-l-lysine and Escherichia coli (E. coli) (JM 101) expressing green fluorescent protein was immobilized. Growth of this culture at 22 and 32 degrees C was monitored by 480 nm light transmission through the tapered fiber. Change in transmission is a measure of change in absorption of the evanescent field. The transmission decreased exponentially with cell growth on the tapered surface. Growth rate was determined and compared favorably with cells grown on the same medium in multiwell plates. Significance of the results is that a tapered fiber sensor can be used effectively for rapid assessment to determine the presence of bacteria by growth.
Maraldo
David
D
Department of Chemical Engineering, Drexel University, 32nd and Chestnut Sts, Philadelphia, PA 19104, USA.
Shankar
P Mohana
PM
Mutharasan
Raj
R
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D049109:Cell Proliferation; D015169:Colony Count, Microbial / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins