WIN 55,212-2 decreases the spatial spread of neocortical excitation in vitro.
The 'intrinsic optical signal' was used to monitor neuronal network excitability. The cannabinoid receptor type 1 agonist WIN 55,212-2 reduced the intensity and the spatial spread of the intrinsic optical signal and prolonged its kinetics in the rat neocortex in vitro. These effects were antagonized by the cannabinoid receptor antagonist SR141716A. Thus, our results suggest that neocortical network activity is modulated via the activation of cannabinoid receptors. The decrease of neocortical network excitability in the present study is probably due to a decreased excitability of glutamatergic neurons.
Becker
Klaus
K
Max-Planck Institute of Psychiatry, Clinical Neuropharmacology, Kraepelinstrasse 2, 80804 Munich, Germany. becker@mpipsykl.mpg.de
Eder
Matthias
M
Zieglgänsberger
Walter
W
Dodt
Hans-Ulrich
HU
D000818:Animals; D000831:Animals, Newborn; D048588:Benzoxazines; D013181:Cortical Spreading Depression / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D003952:Diagnostic Imaging / Q000379:methods; D004558:Electric Stimulation / Q000379:methods; D066298:In Vitro Techniques; D009025:Morpholines / Q000494:pharmacology*; D009281:Naphthalenes / Q000494:pharmacology*; D019579:Neocortex / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists*; D013997:Time Factors
D048588:Benzoxazines; D009025:Morpholines; D009281:Naphthalenes; D043884:Receptor, Cannabinoid, CB1; C070417:(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone