Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes.
This study investigated the presence of cytokines interferon (IFN)-gamma, interleukins (IL) -6 and -8 in serum of cattle and buffaloes infected with Fasciola gigantica from one to 16 weeks post-infection to determine their T cell response during infection. The concentration of these cytokines was determined by sandwich enzyme-linked immunosorbent assay (ELISA). No IFN-gamma was detected in these animals while IL-6 was elevated from one to 16 weeks postinfection. Levels of IL-8 were also elevated in infected buffaloes from one to 16 weeks post-infection. A predominantly T helper (Th) 2 response which started early in the infection was apparently present in cattle and buffaloes in this study which was characterised by IL-6. IL-8 production could be another mechanism of immune response in buffaloes during infection with F. gigantica.
Molina
Elizabeth C
EC
School of Tropical Veterinary Science, James Cook University, Townsville, Queensland 4811, Australia. elizabeth.molina@jcu.edu.au
D000818:Animals; D002020:Buffaloes / Q000097:blood / Q000276:immunology / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology*; D016207:Cytokines / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D011897:Random Allocation
D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D007371:Interferon-gamma