The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Poliovirus provides a well-characterized system for understanding how nonenveloped viruses enter and infect cells. Upon binding its receptor, poliovirus undergoes an irreversible conformational change to the 135S cell entry intermediate. This transition involves shifts of the capsid protein beta barrels, accompanied by the externalization of VP4 and the N terminus of VP1. Both polypeptides associate with membranes and are postulated to facilitate entry by forming a translocation pore for the viral RNA. We have calculated cryo-electron microscopic reconstructions of 135S particles that permit accurate placement of the beta barrels, loops, and terminal extensions of the capsid proteins. The reconstructions and resulting models indicate that each N terminus of VP1 exits the capsid though an opening in the interface between VP1 and VP3 at the base of the canyon that surrounds the fivefold axis. Comparison with reconstructions of 135S particles in which the first 31 residues of VP1 were proteolytically removed revealed that the externalized N terminus is located near the tips of propeller-like features surrounding the threefold axes rather than at the fivefold axes, as had been proposed in previous models. These observations have forced a reexamination of current models for the role of the 135S particle in transmembrane pore formation and suggest testable alternatives.
Bubeck
Doryen
D
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Filman
David J
DJ
Cheng
Naiqian
N
Steven
Alasdair C
AC
Hogle
James M
JM
Belnap
David M
DM
D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000737:chemistry / Q000472:pathogenicity* / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure
D036022:Capsid Proteins; C442541:VP2 protein, Poliovirus; C442542:VP3 protein, Poliovirus