title: Functional characterization of KlHEM13, a hypoxic gene of Kluyveromyces lactis.
abstract: The KlHEM13 gene of Kluyveromyces lactis encoding the coproporphyrinogen oxidase (EC 1.3.3.3), an oxygen-requiring enzyme that catalyzes the sixth step of heme biosynthesis, was cloned and functionally characterized. The coding and upstream regions of KlHEM13 were analyzed and the putative cis regulatory elements were discussed in relation to the mechanisms of regulation of this hypoxic gene in K. lactis.
authors.affiliation: Departmento de Biología Celular y Molecular, Universidad de A Coruña, F. Ciencias, Spain.
authors.forename: Moisés
authors.initials: M
authors.lastname: Blanco
authors.forename: Manuel
authors.initials: M
authors.lastname: Becerra
authors.forename: M Isabel
authors.initials: MI
authors.lastname: González-Siso
authors.forename: M Esperanza
authors.initials: ME
authors.lastname: Cerdán
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003304:Coproporphyrinogen Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006418:Heme / Q000096:biosynthesis; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D006418:Heme; D003304:Coproporphyrinogen Oxidase; D010100:Oxygen