title: Taura syndrome virus from Belize represents a unique variant.
abstract: A Taura syndrome virus (TSV) isolate from cultured Penaeus vannamei grown in Belize, Central America was characterized and shown to be a unique isolate. Mortality rates in laboratory infections of specific pathogen-free (SPF) P. vannamei, reactivity of the virus with monoclonal antibody (MAb) 1A1 and phylogenetic analysis demonstrated that the Belize isolate (BLZ02TSV) is a new valiant of TSV. The Hawaiian 1994 TSV isolate (HI94TSV, GenBank AF277675) was used as the reference isolate for these studies. Laboratory infections of SPF P. vannamei with BLZ02TSV demonstrated higher mortalities and earlier onset of mortalities compared to infections with HI94TSV. Shrimp tissues infected with BLZ02TSV reacted with a TSV-specific gene probe by in situ hybridization and were positive by RT-PCR using TSV diagnostic primers, thus indicating that the isolate was TSV. However, Western blot analysis and immunohistochemistry using MAb 1A1 demonstrated that BLZ02TSV did not react with the antibody, suggestive of changes in the VP1 region of the genome that codes for the polypeptide to which MAb 1A1 binds. Phylogenetic analysis of a 1.3 kbp fragment of the TSV VP1 capsid region revealed that BLZ02TSV represents a distinct group among more than 29 isolates of TSV studied thus far. This research demonstrates that BLZ02TSV is a unique isolate of TSV and reiterates a problem related to the use of MAb 1A1 for detection of TSV in clinical specimens.
authors.affiliation: Aquaculture Pathology Laboratory, Department of Veterinary Science and Microbiology, The University of Arizona, 1117 East Lowell Street, Tucson, Arizona 85721, USA.
authors.forename: Heidi S
authors.initials: HS
authors.lastname: Erickson
authors.forename: Bonnie T
authors.initials: BT
authors.lastname: Poulos
authors.forename: Kathy F J
authors.initials: KF
authors.lastname: Tang
authors.forename: Deborah
authors.initials: D
authors.lastname: Bradley-Dunlop
authors.forename: Donald V
authors.initials: DV
authors.lastname: Lightner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D017756:Aquaculture; D001483:Base Sequence; D001531:Belize; D015153:Blotting, Western; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers