Inhibitory effect of coumarins from Weigela subsessilis on low density lipoprotein oxidation.
Oxidation of low density lipoprotein (LDL) is thought to be a major factor in the pathophysiology of atherosclerosis. In the present study, we found that coumarins isolated from Weigela subsessilis (Caprifoliaceae) inhibited LDL oxidation mediated by either catalytic copper ions (Cu2+) or free radicals generated with the azo compound 2,2'-azobis-(2-amidinopropane)dihydrochloride (AAPH). Of the coumarins tested, scopoletin (1) and cleomiscosin A (2) increased the lag time of conjugated diene formation and inhibited the generation of thiobarbituric acid reactive substances (TBARS) in a dose-dependent manner. In addition, it was found that compounds 1 and 2 had the capacity to protect the fragmentation of apolipoprotein B-100 (apoB-100). These results suggest that W. subsessilis and its active coumarins, 1 and 2, may have a role to play in preventing the LDL oxidation involved in atherogenesis.
Thuong
Phuong Thien
PT
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Na
MinKyun
M
Su
Nguyen Duy
ND
Seong
Rack Seon
RS
Lee
Young Mi
YM
Sok
Dai Eun
DE
Bae
KiHwan
K
D027922:Caprifoliaceae; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D018547:Plant Stems
D003374:Coumarins; D008077:Lipoproteins, LDL; D010936:Plant Extracts