Synthesis and screening of a small library of proline-based biodendrimers for use as delivery agents.
A small library of defined peptide dendrimers based on polyproline sequences was designed to demonstrate the feasibility of generating a new type of polymeric agent for therapeutic use. Structural modifications to dendrimer surfaces further enriched the diversity of the library. Data show that the prolinerich dendrimers can be internalized in human epithelial (HeLa) cells, demonstrating the importance of the dendrimeric motif. The promising results described herein suggest that controlled modification of the dendrimer surface should eventually yield proline dendrimers with therapeutic potential.
Sanclimens
Glòria
G
Barcelona Biomedical Research Institute, Barcelona Science Park, University of Barcelona, Josep Samitier 1, 08028 Barcelona, Spain.
Shen
Hong
H
Giralt
Ernest
E
Albericio
Fernando
F
Saltzman
Mark William
MW
Royo
Miriam
M
D000818:Animals; D019556:COS Cells; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000378:metabolism; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression; D015723:Gene Library; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D011392:Proline / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013696:Temperature; D001616:beta-Galactosidase / Q000378:metabolism
D003470:Culture Media; D050091:Dendrimers; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D004247:DNA; D011392:Proline; D001616:beta-Galactosidase