Chromosomal aberrations induced by double strand DNA breaks.
It has been suggested that introduction of double strand DNA breaks (DSBs) into mammalian chromosomes can lead to gross chromosomal rearrangements through improper DNA repair. To study this phenomenon, we employed a model system in which a double strand DNA break can be produced in human cells in vivo at a predetermined location. The ensuing chromosomal changes flanking the breakage site can then be cloned and characterized. In this system, the recognition site for the I-SceI endonuclease, whose 18 bp recognition sequence is not normally found in the human genome, is placed between a strong constitutive promoter and the Herpes simplex virus thymidine kinase (HSV-tk) gene, which serves as a negative selectable marker. We found that the most common mutation following aberrant DSB repair was an interstitial deletion; these deletions typically showed features of non-homologous end joining (NHEJ), such as microhomologies and insertions of direct or inverted repeat sequences. We also detected more complex rearrangements, including large insertions from adjacent or distant genomic regions. The insertion events that involved distant genomic regions typically represented transcribed sequences, and included both L1 LINE elements and sequences known to be involved in genomic rearrangements. This type of aberrant repair could potentially lead to gene inactivation via deletion of coding or regulatory sequences, or production of oncogenic fusion genes via insertion of coding sequences.
Varga
Tamas
T
Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Navy 8, 8901 Wisconsin Ave., Bethesda, MD 20889-5105, USA.
Aplan
Peter D
PD
D015139:Blotting, Southern; D002460:Cell Line; D002869:Chromosome Aberrations; D019457:Chromosome Breakage / Q000235:genetics*; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015321:Gene Rearrangement; D006801:Humans; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D013937:Thymidine Kinase; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific