[Changes in activity of transcription factors in the cells of vascular endothelium as a pathological mechanism of its dysfunction in essential hypertension].
OBJECTIVE
To study activity of transcription factor (TF)--the nuclear factor kappa B (NF-kB)--and its role in formation of endothelial dysfunction (ED) in patients with essential hypertension (EH).


METHODS
The examination covered 74 patients with EH stage I-II with the first-third degree of arterial hypertension (AH) by WHO/IHS classification (48 males, 36 females, mean age 46.39 +/- 3.47 years, free of symptomatic AH, diabetes mellitus, hyperlipidemia, IHD, congestive cardiac failure or other diseases with proved formation of ED or NF-kB activity alteration) and 26 healthy persons (control group). Venous occlusive plethysmography of the arms for assessment of blood flow in brachial arteries with measurement of blood flow velocity at rest and after the test for reactive hyperemia was made. Functional state of vascular endothelium was estimated according to mechanic sensitivity coefficient (MSC). Molecule-1 intercellular adhesion (ICAM-1) and E-selectin production were measured in blood. By ED degree, the patients were divided into 3 groups: group 1--patients with EH and mild ED (MSC > M +/- sigma), group 2--patients with EH and moderate ED (MSC = M +/- sigma), group 3--patients with EH and severe ED.


RESULTS
In EH patients, MSC is lower than in healthy controls, serum ICAM-1 level and E-selectin production were higher (by 26.99 and 50.77%, respectively). E-selectin was higher in group 2 than in group 2 by 32.70%, in group 3--by 55.84 and 17.44% than in groups 1 and 2, respectively. Serum level of ICAM-1 in group 3 was by 25.31% in group 2 by 12.02% higher than in group 1. The regressive analysis revealed that there is a significant correlation between MSC and ICAM-1 in the serum. The level of ICAM-1 is an independent factor determining MSC.


CONCLUSIONS
EH patients demonstrate activation of HF-kB transcription factor in the cells of vascular endothelium, manifesting with an E-selectin production rise and elevation of serum ICAM-1. Activation of NF-kB in the cells of vascular endothelium is an independent and pathophysiologically significant factor promoting formation of ED in EH patients.
Polivoda
S N
SN
Cherepok
A A
AA
D001783:Blood Flow Velocity; D019040:E-Selectin / Q000097:blood; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D014157:Transcription Factors / Q000235:genetics*
D019040:E-Selectin; D014157:Transcription Factors; D018799:Intercellular Adhesion Molecule-1