Determination of methyltestosterone using flow injection with chemiluminescence detection.
Methyltestosterone (MT) enhanced the chemiluminescence of potassium permanganate-sodium thiosulphate system in sulphuric acid medium, and this was used as the basis of a novel flow-injection chemiluminescence method for the determination of methyltesterone. Under optimum conditions, the linear response of MT was in the range 3.0 x 10(-8)-8.0 x 10(-6) g/mL, and the detection limit for determination of MT was 1.0 x 10(-8) g/mL. The method was applied to the analysis of fish with recovery in the range 88.0-113.8% with an average RSD of 1.66% (n = 8).
Xie
Zenghong
Z
Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, People's Republic of China.
Ouyang
Xiaoqing
X
Guo
Liangqia
L
Lin
Xucong
X
Chen
Guonan
G
D000818:Animals; D002138:Calibration; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D008777:Methyltestosterone / Q000032:analysis*; D011196:Potassium Permanganate; D013885:Thiosulfates
D013885:Thiosulfates; D011196:Potassium Permanganate; C017717:sodium thiosulfate; D008777:Methyltestosterone