Typing class I HLA-A gene using a nested PCR-RFLP procedure.
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Castelli
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EC
TOXICAN, Departamento de Patologia, Faculdade de Medicina, Universidade Estadual Paulista, 186180-000 Botucatu, SP, Brasil. erickcastelli@terra.com.br
Gil
D S
DS
Veiga
L C S
LC
de Camargo
J L V
JL
D000483:Alleles; D005805:Genes, MHC Class I / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods
D015234:HLA-A Antigens