title: Microdissection-derived murine mcb probes from somatic cell hybrids.
abstract: The multicolor-banding (mcb) technique is a fluorescence in situ hybridization (FISH)-banding approach, which is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first three available mcb-probe sets for the Mus musculus chromosomes 3, 6, and 18. In the present work, the creation of the microdissection libraries was done for the first time on mouse/human somatic cell hybrids. During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes.
authors.affiliation: Institut für Human Genetics and Anthropology, Postfach, D-07740 Jena, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trifonov
authors.forename: Constanze
authors.initials: C
authors.lastname: Karst
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Kristin
authors.initials: K
authors.lastname: Mrasek
authors.forename: Susanne
authors.initials: S
authors.lastname: Michel
authors.forename: Philip
authors.initials: P
authors.lastname: Avner
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000648:ultrastructure*; D006822:Hybrid Cells / Q000648:ultrastructure*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D042282:Microdissection