title: Blimp1 is a critical determinant of the germ cell lineage in mice.
abstract: Germ cell fate in mice is induced in pluripotent epiblast cells in response to signals from extraembryonic tissues. The specification of approximately 40 founder primordial germ cells and their segregation from somatic neighbours are important events in early development. We have proposed that a critical event during this specification includes repression of a somatic programme that is adopted by neighbouring cells. Here we show that Blimp1 (also known as Prdm1), a known transcriptional repressor, has a critical role in the foundation of the mouse germ cell lineage, as its disruption causes a block early in the process of primordial germ cell formation. Blimp1-deficient mutant embryos form a tight cluster of about 20 primordial germ cell-like cells, which fail to show the characteristic migration, proliferation and consistent repression of homeobox genes that normally accompany specification of primordial germ cells. Furthermore, our genetic lineage-tracing experiments indicate that the Blimp1-positive cells originating from the proximal posterior epiblast cells are indeed the lineage-restricted primordial germ cell precursors.
authors.affiliation: Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2-2-3 Minatojima-minamimachi, Kobe, Hyogo 650-0047, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Ohinata
authors.forename: Bernhard
authors.initials: B
authors.lastname: Payer
authors.forename: Dónal
authors.initials: D
authors.lastname: O'Carroll
authors.forename: Katia
authors.initials: K
authors.lastname: Ancelin
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ono
authors.forename: Mitsue
authors.initials: M
authors.lastname: Sano
authors.forename: Sheila C
authors.initials: SC
authors.lastname: Barton
authors.forename: Tetyana
authors.initials: T
authors.lastname: Obukhanych
authors.forename: Michel
authors.initials: M
authors.lastname: Nussenzweig
authors.forename: Alexander
authors.initials: A
authors.lastname: Tarakhovsky
authors.forename: Mitinori
authors.initials: M
authors.lastname: Saitou
authors.forename: M Azim
authors.initials: MA
authors.lastname: Surani
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D049109:Cell Proliferation; D005775:Gastrula / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D051379:Mice; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1