Peptide-conjugated quantum dots: imaging the angiotensin type 1 receptor in living cells.
Peptide-quantum dot conjugates have been prepared by attaching angiotensin II (Ang II) to cadmium selenide/zinc sulfide core-shell nanocrystals using an 1-[3-(Dimethyamino)propyl]-3-ethylcarbo diimide hydrochloride (EDC) coupling. These conjugates have been used to image angiotensin I-expressing Chinese hamster ovary (CHO) cells in vitro. When CHO cells were incubated with Ang II before incubating with Ang II-conjugated quantum dots, we were able to block the binding of the dots. The Ang II-quantum dot conjugates did not bind to parental cells and showed similar staining patterns when compared with a commercially available Ang II Alexa 488 conjugate.
Tomlinson
Ian D
ID
Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
Mason
John N
JN
Blakely
Randy D
RD
Rosenthal
Sandra J
SJ
D000804:Angiotensin II / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003952:Diagnostic Imaging / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D045663:Quantum Dots; D011945:Receptors, Angiotensin / Q000032:analysis*
D010455:Peptides; D011945:Receptors, Angiotensin; D000804:Angiotensin II