Molecular studies of loss of heterozygosity in Chinese sporadic retinoblastoma patients.
BACKGROUND
Retinoblastoma, an embryonic neoplasm of retinal origin, is the most common and severe intra-ocular tumor affecting infants and young children.


METHODS
Loss of heterozygosity (LOH) on chromosome 13 was investigated in 16 Chinese sporadic RB patients, using 14 microsatellite markers spanning the complete chromosome 13, to determine whether those alterations different from the alteration of RB1 gene on 13q14 may play a role in the development of RB. Loss of RB1 allele is commonly encountered in sporadic RB. Microdissected RB tissues and their matched blood DNAs were analyzed for PCR-based LOH by using fluorescence-based DNA sequencing technology.


RESULTS
Of 16 RB cases, 13 showed LOH on chromosome 13. The frequency of LOH on 13q14 was about 75% (12/16), consistent with other reports. Investigation of parental origin of lost RB1 alleles showed that, in all these cases, the paternal alleles were preferentially lost. Aside from the RB1 locus, other regions with the frequency of LOH above 30% in these tumors were D13S265, D13S158, D13S170, D13S218, D13S285 and D13S159. In particular, the D13S265 locus at 13q31-32 showed the highest rate of allele loss (64%, 9/14 informative cases), suggesting the presence of 1 or several genes whose loss of function may contribute to the development of RB. Comparison of the genotypic characteristics of 3 sites of frequent LOH (D13S153, D13S263 and D13S265) with the clinicopathological phenotype, respectively, showed that LOH of each locus was preferentially associated with a significantly younger age at diagnosis of RB.


CONCLUSIONS
LOH analysis at some specific loci on chromosome 13 may be of a value in RB patients as diagnostic markers.
Zhang
Xiao L
XL
Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University, Chongqing, PR China. xlzhang227@yahoo.com.cn
Fu
Wei L
WL
Zhao
Hong X
HX
Zhou
Lai X
LX
Huang
Jun F
JF
Wang
Jiang H
JH
D000483:Alleles; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D019656:Loss of Heterozygosity; D008297:Male; D012175:Retinoblastoma / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics*