Enamel matrix derivative gel stimulates signal transduction of BMP and TGF-{beta}.
It has been shown that Emdogain Gel (Emd-Gel) containing enamel matrix proteins promotes biomineralization, such as osteogenesis and cementogenesis, during the regeneration of periodontal tissues. However, the growth factors involved in these activities of Emd-Gel remain unclear. In this study, Emd-Gel was fractionated into 22 sub-fractions by size exclusion chromatography. The osteoinductive factors, TGF-beta and BMP, were examined by a specific luciferase reporter gene assay. In the unfractionated Emd-Gel, TGF-beta-like activity was detected, while BMP activity was not. In contrast, in the fractionated Emd-Gel samples, TGF-beta-like activity was detected from fractions 8 to 13, and BMP-like activity was detected from fractions 4 to 6. Also, it was confirmed that the BMP-like activity in Emd-Gel was inhibited by authentic TGF-beta1 and TGF-beta-like activity. These results indicate that Emd-Gel contains both TGF-beta- and BMP-like growth factors that contribute to the induction of biomineralization during periodontal regeneration.
Suzuki
S
S
Department of Periodontics and Endodontics, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. suzuki-shin@tsurumi-u.ac.jp
Nagano
T
T
Yamakoshi
Y
Y
Gomi
K
K
Arai
T
T
Fukae
M
M
Katagiri
T
T
Oida
S
S
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000302:isolation & purification; D002460:Cell Line; D002850:Chromatography, Gel; D003746:Dental Enamel Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D008156:Luciferases; D049408:Luminescent Agents; D008168:Lung / Q000166:cytology; D051379:Mice; D008907:Mink; D032446:Myoblasts; D010012:Osteogenesis / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000187:drug effects* / Q000302:isolation & purification / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D003746:Dental Enamel Proteins; D049408:Luminescent Agents; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C109069:enamel matrix proteins; D008156:Luciferases