title: Increased levels of platelet-derived microparticles in patients with diabetic retinopathy.
abstract: Diabetic retinopathy is caused by capillary occlusions. Platelet-derived microparticles (PMPs) stimulate the coagulation cascade and increase leukocyte and endothelial cell adhesions, both of which are key events in the development of diabetic retinopathy. However, the correlation between the levels of PMPs and diabetic retinopathy has not been precisely determined. The PMPs levels and the expression of platelet CD62P and CD63 were measured in 92 diabetic patients. The level of PMPs was significantly correlated with the expression of CD62P (r = 0.76, P < 0.0001) and CD63 (r = 0.71, P < 0.0001). The mean level of PMPs in diabetics (507+/-15/10(4) platelets (plt), mean+/-S.E.) was significantly higher than that in normal. The PMPs levels increased with the progression of the diabetic retinopathy; 480+/-28/10(4) plt in diabetic patients without retinopathy (n = 25), 504+/-40/10(4) plt with mild or moderate non-proliferative diabetic retinopathy (n = 13), 512+/-29/10(4) plt with severe non-proliferative diabetic retinopathy (n = 25), and 528+/-25/10(4) plt with proliferative diabetic retinopathy (n=29). The PMPs level in patients with non-perfused retinal areas (582+/-27/10(4) plt, n = 24) was significantly higher than patients without non-perfused areas (469+/-23/10(4) plt, n = 30; P = 0.0096) and without diabetic retinopathy (P = 0.024). These high correlations indicate that increased levels of PMPs may accelerate diabetic retinopathy.
authors.affiliation: Department of Ophthalmology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, Osaka 570-8507, Japan. ogata@takii.kmu.ac.jp
authors.forename: Nahoko
authors.initials: N
authors.lastname: Ogata
authors.forename: Masahito
authors.initials: M
authors.lastname: Imaizumi
authors.forename: Shosaku
authors.initials: S
authors.lastname: Nomura
authors.forename: Akira
authors.initials: A
authors.lastname: Shozu
authors.forename: Miwa
authors.initials: M
authors.lastname: Arichi
authors.forename: Masato
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Miyo
authors.initials: M
authors.lastname: Matsumura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood; D001792:Blood Platelets / Q000473:pathology*; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D003930:Diabetic Retinopathy / Q000097:blood*; D018450:Disease Progression; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D010316:Particle Size; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; D002784:Cholesterol; D006442:Glycated Hemoglobin; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; C554586:CD63 protein, human