title: The effect of N-acetyl-l-cysteine and ascorbic acid on visible-light-irradiated camphorquinone/N,N-dimethyl-p-toluidine-induced oxidative stress in two immortalized cell lines.
abstract: Recent studies have revealed that visible-light (VL)-irradiated camphorquinone (CQ), in the presence of a tertiary amine (e.g., N,N-dimethyl-p-toluidine, DMT), generates initiating radicals that may indiscriminately react with molecular oxygen forming reactive oxygen species (ROS). In this study, the ability of the antioxidants N-acetyl-l-cysteine (NAC) and ascorbic acid (AA) to reduce intracellular oxidative stress induced by VL-irradiated CQ/DMT or VL-irradiated hydrogen peroxide (H(2)O(2)) was assessed in an immortalized Murine cementoblast cell line (OCCM.30) and an immortalized Murine fibroblast cell line, 3T3-Swiss albino (3T3). Intracellular oxidative stress was measured with the membrane permeable dye, 2',7'-dichlorodihydrofluorescein diacetate (H(2)DCF-DA). VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) each produced significantly (p<0.001) elevated intracellular oxidative levels in both cell types compared to intracellular ROS levels in VL-irradiated untreated cells. OCCM.30 cementoblasts were found to be almost twice as sensitive to VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) treatment compared to 3T3 fibroblasts. Furthermore, 10mm NAC and 10mm AA each eliminated oxidative stress induced by VL-irradiated CQ/DMT and VL-irradiated H(2)O(2) in both cell types. Our results suggest that NAC and AA may effectively reduce or eliminate oxidative stress in cells exposed to VL-irradiated CQ/DMT following polymerization.
authors.affiliation: Department of Restorative Dentistry/Division of Operative Dentistry, School of Dentistry, University of Washington, Box 357456, Seattle, WA, 98195-7456 USA.
authors.forename: D
authors.initials: D
authors.lastname: Pagoria
authors.forename: W
authors.initials: W
authors.lastname: Geurtsen
mesh_terms: D016475:3T3 Cells; D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002460:Cell Line; D003188:Composite Resins / Q000494:pharmacology; D003739:Dental Cementum / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D003764:Dental Materials / Q000494:pharmacology; D004347:Drug Interactions; D008027:Light; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D017382:Reactive Oxygen Species; D013729:Terpenes; C048339:camphoroquinone; D001205:Ascorbic Acid; D000111:Acetylcysteine