title: Effects of excretory/secretory products of Haemonchus contortus on cell vacuolation.
abstract: Excretory/secretory (ES) products of the gastric nematode, Haemonchus contortus, have been implicated in the inhibition of gastric acid secretion which follows infection. Parietal cell vacuolation has been observed in abomasal sections from parasitised sheep, and ES prepared in vitro has been reported to cause vacuolation and to increase neutral red (NR) uptake in epithelial cell cultures. We have used the latter approach to examine, at the cellular level, the effects of ES prepared from L3 and adult nematodes. Both NR uptake and cellular vacuolation were increased following exposure to larval or adult ES products. ES preparations from adult worms induced more extensive vacuolation then those from L3 worms and, as with VacA treatment, adherent cells remained viable despite high levels of vacuolation. Whereas VacA-induced vacuolation resulted in NR uptake predominantly localised in vacuoles, this appeared not to be the case with ES-induced vacuolation, suggesting that different mechanisms might be involved. Both ES and VacA exposure was associated with an increased rate of cell detachment.
authors.affiliation: Institute of Parasitology and Zoology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Wien, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Huber
authors.forename: Heinrich
authors.initials: H
authors.lastname: Prosl
authors.forename: Anja
authors.initials: A
authors.lastname: Joachim
authors.forename: Heather V
authors.initials: HV
authors.lastname: Simpson
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Pedley
mesh_terms: D000018:Abomasum / Q000469:parasitology; D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006189:Haemonchus / Q000378:metabolism*; D006367:HeLa Cells; D015801:Helminth Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007814:Larva / Q000378:metabolism; D012756:Sheep; D014617:Vacuoles / Q000187:drug effects*
chemical_list: D001685:Biological Factors; D015801:Helminth Proteins