The effect of two different bioreactors on the neocartilage formation in type II collagen modified polyester scaffolds seeded with chondrocytes.
The effect of dynamic culture conditions on neocartilage formation in type II collagen modified polyester scaffolds was studied. Porcine or human articular chondrocytes were seeded in the scaffolds. The cell-scaffold constructs were cultivated statically, in a rotating-type bioreactor or in a shaker for up to 4 weeks. The cell proliferation, morphology, NO production, synthesis of proteoglycans and collagen, and mechanical properties were evaluated. The results demonstrated that the rotating-type bioreactor promoted the growth of primary porcine chondrocytes, helped to maintain their phenotype, and increased the production of extracellular matrix. The constructs also had the largest dynamic compressive modulus. In the static condition, chondrocytes occupied only the outer margin of the cell-polymer constructs. The poor mass transfer in static condition may have caused a lower pH value in the middle of the constructs and lead further to faster scaffold degradation as well as the weakest neocartilage. Constructs in the shaker produced the highest amount of NO as well as the lowest amount of cells and matrix production. Human or porcine chondrocytes of the second passage seeded in scaffolds were much less viable, with the largest amount of cells and matrix when cultured in rotating-type bioreactors. A larger seeding density was required to form neocartilage from passaged adult chondrocytes.
Hsu
Shan-hui
SH
Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Kuo
Chi-Ching
CC
Yen
Hung-Jen
HJ
Whu
Shu Wen
SW
Tsai
Ching-Lin
CL
D000704:Analysis of Variance; D000818:Animals; D019149:Bioreactors; D049109:Cell Proliferation; D019902:Chondrocytes / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism*; D046508:Culture Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000378:metabolism; D011091:Polyesters; D013314:Stress, Mechanical; D013552:Swine; D023822:Tissue Engineering
D024043:Collagen Type II; D011091:Polyesters; D009569:Nitric Oxide