Expression of an RNAi-resistant SLBP restores proper S-phase progression.
Metazoan histone mRNAs are unique in that they lack a 3'-polyadenylated tail, but instead end in a conserved stem loop that is bound by SLBP (stem-loop binding protein). SLBP is required for efficient histone mRNA synthesis and translation. Removal of SLBP by RNA interference causes an increase in the number of cells in S-phase and this effect can be reversed by expressing an exogenous SLBP resistant to the small interfering RNA. Cells with decreased SLBP levels progress slowly through S-phase when released from a double-thymidine block. Thus SLBP is required for efficient DNA replication probably because a decreased ability to assemble chromatin results in a decrease in the rate of DNA replication.
Wagner
E J
EJ
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, NC 27599, USA.
Berkow
A
A
Marzluff
W F
WF
D000818:Animals; D002460:Cell Line; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D016196:S Phase; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics* / Q000378:metabolism*
D009687:Nuclear Proteins; C092663:SLBP protein, human; D039221:mRNA Cleavage and Polyadenylation Factors