Effects of aliphatic acids, furfural, and phenolic compounds on Debaryomyces hansenii CCMI 941.
Debaryomyces hansenii is a polyol overproducing yeast that can have a potential use for upgrading lignocellulosic hydrolysates. Therefore, the establishment of its tolerance to metabolic inhibitors found in hydrolysates is of major interest. We studied the effects of selected aliphatic acids, phenolic compounds, and furfural. Acetic acid favored biomass production for concentrations <6.0 g/L. Formic acid was more toxic than acetic acid and induced xylitol accumulation (maximum yield of 0.21 g/g of xylose). All tested phenolics strongly decreased the specific growth rate. Increased toxicity was found for hydroquinone, syringaldehyde, and 4-methylcatechol and was correlated to the compound's hydrophobicity. Increasing the amount of furfural led to longer lag phases and had a detrimental effect on specific growth rate and biomass productivity.
Duarte
Luís C
LC
Departamento de Biotecnologia, INETI, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Carvalheiro
Florbela
F
Neves
Inês
I
Gírio
Francisco M
FM
D001203:Ascomycota / Q000145:classification / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000494:pharmacology; D005662:Furaldehyde / Q000494:pharmacology*; D008657:Metabolic Clearance Rate / Q000187:drug effects; D009005:Monosaccharides / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D013045:Species Specificity
D005227:Fatty Acids; D009005:Monosaccharides; D010636:Phenols; D005662:Furaldehyde