Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cells.
We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.
Gunawardana
Subhadra C
SC
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Light Hall, Nashville, Tennessee 37232, USA.
Hara
Manami
M
Bell
Graeme I
GI
Head
W Steven
WS
Magnuson
Mark A
MA
Piston
David W
DW
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology* / Q000196:embryology*; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D019076:Transgenes / Q000235:genetics
D049452:Green Fluorescent Proteins