RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion.
Previous work indicates that matrix metalloproteinase-1 (MMP-1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP-1 knock-down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA oligos tested, one showed a 90% inhibitory effect on MMP-1 gene expression. Stable attenuation of MMP-1 in chondrosarcoma cells was achieved by using a plasmid vector-based siRNA system. Pronounced MMP-1 activity suppression was accompanied by marked inhibition of tumor cell invasion. Cells in which MMP-1 has been stably silenced are useful tools to elucidate the mechanism of MMP-1 mediated tumor metastasis.
Yuan
Jun
J
Department of Orthopaedics, Mayo Clinic, Rochester, MN, USA.
Dutton
Charyl M
CM
Scully
Sean P
SP
D001859:Bone Neoplasms / Q000473:pathology / Q000628:therapy*; D002460:Cell Line; D002813:Chondrosarcoma / Q000473:pathology / Q000628:therapy*; D020868:Gene Silencing; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D010957:Plasmids; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000627:therapeutic use*
D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020781:Matrix Metalloproteinase 1