The role of myostatin and the calcineurin-signalling pathway in regulating muscle mass in response to exercise training in the rainbow trout Oncorhynchus mykiss Walbaum.
Rainbow trout Oncorhynchus mykiss Walbaum were exercised at 0.8 and 1.6 body lengths s(-1) for 18 h a day over a 30 day period. Exercise resulted in a 24-30% increase in the average cross-sectional area of fast muscle fibres relative to tank-rested controls. The concentrations of growth factors and transcription factors hypothesised to play a role in regulating exercise-induced muscle fibre hypertrophy were measured. Exercise training resulted in a minor increase in calcineurin localisation in the nucleus. However, nuclear factor of T-cells 2 (NFAT2) nuclear localisation did not follow a pattern that was consistent with NFAT2-mediated transcriptional activity and changes in calcineurin signaling. The active peptide of myostatin, a negative regulator of muscle growth in mammals, was downregulated in exercise groups relative to tank-rested controls, but only by 6-7%. It was concluded that myostatin and calcineurin signaling do not play a major role in regulating exercise-induced muscle hypertrophy in trout.
Martin
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Gatty Marine Laboratory, School of Biology, University of St Andrews, St Andrews, Scotland KY16 8LB, UK.
Johnston
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D000818:Animals; D019703:Calcineurin / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D051379:Mice; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism; D055435:Myostatin; D017686:Oncorhynchus mykiss / Q000378:metabolism / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D020317:Salmo salar; D015398:Signal Transduction / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000502:physiology*
C525912:Mstn protein, mouse; C525913:Mstn protein, rat; D055435:Myostatin; D016212:Transforming Growth Factor beta; D019703:Calcineurin