title: 1alpha,25-Dihydroxyvitamin D3 modulates the murine antibody response to pneumococcal capsular polysaccharide serotype 3 through IL-12.
abstract: 1alpha,25-Dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)] is a steroid hormone that regulates calcium metabolism. Besides, 1alpha,25(OH)(2)D(3 )also has pronounced immunomodulatory effects: it strongly inhibits dendritic cell (DC) maturation and impairs IL-12 production. We studied the effect of 1alpha,25(OH)(2)D(3 )on the antibody response to pneumococcal capsular polysaccharide (caps-PS) serotype 3. 1alpha,25(OH)(2)D(3) inhibited the IgG2a antibody response to caps-PS serotype 3. Besides, 1alpha,25(OH)(2)D(3) also inhibited IL-12 production and maturation of DC. Anti-IL-12 and exogenous IL-12, respectively, inhibited and stimulated the IgG2a antibody response to caps-PS serotype 3. Exogenous IL-12 abrogated the effect of 1alpha,25(OH)(2)D(3) on the IgG2a antibody response to caps-PS serotype 3, indicating that the effect of 1alpha,25(OH)(2)D(3) on the IgG2a antibody response to caps-PS serotype 3 was mediated through IL-12. In conclusion, we demonstrate that 1alpha,25(OH)(2)D(3) has an inhibitory effect on the IgG2a antibody response to caps-PS serotype 3, and that this effect was mediated trough IL-12.
authors.affiliation: Experimental Laboratory Medicine, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium.
authors.forename: Axel
authors.initials: A
authors.lastname: Jeurissen
authors.forename: Evelyne
authors.initials: E
authors.lastname: Van Etten
authors.forename: Lut
authors.initials: L
authors.lastname: Overbergh
authors.forename: Greet
authors.initials: G
authors.lastname: Wuyts
authors.forename: Hubertine
authors.initials: H
authors.lastname: Heremans
authors.forename: Patrick
authors.initials: P
authors.lastname: Matthys
authors.forename: Jan L
authors.initials: JL
authors.lastname: Ceuppens
authors.forename: Chantal
authors.initials: C
authors.lastname: Mathieu
authors.forename: Xavier
authors.initials: X
authors.lastname: Bossuyt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D016667:Bacterial Capsules / Q000276:immunology*; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D018664:Interleukin-12 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D018664:Interleukin-12; D002117:Calcitriol