Evaluation of inoculum of Candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate.
The effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of Candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/L), xylose (30 g/L), or a mixture of glucose (2 g/L) and xylose (30 g/L). The inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 IU/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0.203 IU/mgprot) when compared with an inoculum-grown medium containing only xylose. The improvement in enzyme activities resulted in higher values of xylitol yield (0.56 g/g) and productivity (0.46 g/[L.h]) after 48 h of fermentation.
da Silva
Débora Danielle Virgínio
DD
Department of Biotechnology, Faculty of Chemical Engineering of Lorena, FAENQUIL, Rodovia Itajuba-Lorena, Km 74.5, Caixa Postal 116, 12600-970, Lorena, SP, Brazil.
das Graças de Almeida Felipe
Maria
M
de Mancilha
Ismael Maciel
IM
da Silva
Sílvio Silvério
SS
D000449:Aldehyde Reductase / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D003470:Culture Media / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D050542:D-Xylulose Reductase; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D014993:Xylitol / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D003470:Culture Media; D014994:Xylose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D005947:Glucose; D014993:Xylitol