Effects of T-lymphocyte depletion on muscle fibrosis in the mdx mouse.
Duchenne muscular dystrophy was initially described as a myosclerosis because of the conspicuous progression of interstitial fibrosis. Using the mdx mouse homologue, we have shown previously that the accumulation of intramuscular collagen is profoundly influenced by the presence or absence of T lymphocytes. Here we have used thymectomy and antibody depletion to examine the effect of ablating CD4 or CD8 or both subsets of T lymphocytes on skeletal muscle fibrosis in mdx and C57BL10 (wild-type) mice. Depletion of either or both subsets at 4 weeks of age did not influence fibrosis in mdx mice, as determined by measuring hydroxyproline levels and collagen deposition in diaphragm. Additionally, expression of transforming growth factor-beta1, which is implicated in collagen deposition, either decreased (mdx mice) or increased (C57BL/10 mice) after double CD4/8 depletion. Our data suggest that depletion of lymphoid cells may affect the tight regulatory control of transforming growth factor-beta1, with possible pleiotropic effects, and more importantly, that the fibrotic process is self-sustaining from a very early stage.
Morrison
Jamie
J
Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
Palmer
Donald B
DB
Cobbold
Stephen
S
Partridge
Terence
T
Bou-Gharios
George
G
D000818:Animals; D003238:Connective Tissue / Q000378:metabolism; D005355:Fibrosis / Q000473:pathology*; D005434:Flow Cytometry; D006909:Hydroxyproline / Q000032:analysis; D008212:Lymphocyte Depletion; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000473:pathology*; D009137:Muscular Dystrophy, Animal / Q000276:immunology* / Q000503:physiopathology; D013601:T-Lymphocytes / Q000276:immunology*; D013934:Thymectomy; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1
C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D006909:Hydroxyproline