title: Oct2 transcription factors in fish--a comparative genomic analysis.
abstract: The Oct2 transcription factor is important in driving expression of the IgH locus of the channel catfish, Ictalurus punctatus. Two isoforms, catfish Oct2alpha and Oct2beta, have been characterized at the level of expression and function, but little is known of the structure of the Oct2 gene in catfish. To gain insight into the diversity of Oct2 gene structure and expression in the teleost fish, a comparative genomic analysis of Oct2 was undertaken in the pufferfish (Fugu rubripes) and the zebrafish (Danio rerio). The orthologues of zebrafish and Fugu Oct2 were identified, and share with catfish Oct2 the expression of a limited number (two in zebrafish, three in Fugu) of isotypes produced by alternative pathways of RNA processing. The alternatively spliced variants of catfish Oct2 showed a different pattern of exon use from those of Fugu and zebrafish. The analysis also identified a novel homologue of Oct2 in both zebrafish and Fugu. This homologue, termed Oct2x, shares similarities to both Oct1 and Oct2. A phylogenetic analysis of the relationships of Oct2x gave an unexpected result, with Oct2x occupying a position basal to the Oct gene families of both vertebrates and Drosophila.
authors.affiliation: Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
authors.forename: Mara L
authors.initials: ML
authors.lastname: Lennard
authors.forename: Melanie R
authors.initials: MR
authors.lastname: Wilson
authors.forename: Norman W
authors.initials: NW
authors.lastname: Miller
authors.forename: L William
authors.initials: LW
authors.lastname: Clem
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Warr
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D005075:Biological Evolution; D004330:Drosophila / Q000235:genetics; D005091:Exons / Q000235:genetics; D005399:Fishes / Q000235:genetics* / Q000276:immunology; D023061:Gene Order; D016678:Genome / Q000235:genetics*; D006801:Humans; D007059:Ictaluridae / Q000235:genetics; D050813:Octamer Transcription Factor-2 / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D017422:Sequence Analysis, DNA; D023741:Takifugu / Q000235:genetics; D014714:Vertebrates / Q000235:genetics; D015027:Zebrafish / Q000235:genetics
chemical_list: D050813:Octamer Transcription Factor-2; D020033:Protein Isoforms