title: On the interaction between amiloride and its putative alpha-subunit epithelial Na+ channel binding site.
abstract: The epithelial Na+ channel (ENaC) belongs to the structurally conserved ENaC/Degenerin superfamily. These channels are blocked by amiloride and its analogues. Several amino acid residues have been implicated in amiloride binding. Primary among these are alphaSer-583, betaGly-525, and gammaGly-542, which are present at a homologous site within the three subunits of ENaC. Mutations of the beta and gamma glycines greatly weakened amiloride block, but, surprisingly, mutation of the serine of the alpha subunit resulted in moderate (<5-fold) weakening of amiloride K(i). We investigated the role of alphaSer-583 in amiloride binding by systematically mutating alphaSer-583 and analyzing the mutant channels with two-electrode voltage clamp. We observed that most mutations had moderate effects on amiloride block, whereas those introducing rings showed dramatic effects on amiloride block. In addition, mutations introducing a beta-methyl group at this site altered the electric field of ENaC, affecting both amiloride binding and the voltage dependence of channel gating. We also found that the His mutation, in addition to greatly weakening amiloride binding, appends a voltage-sensitive gate within the pore of ENaC at low pH. Because diverse residues at alpha583, such as Asn, Gln, Ser, Gly, Thr, and Ala, have similar amiloride binding affinities, our results suggest that the wild type Ser side chain is not important for amiloride binding. However, given that some alphaSer-583 mutations affect the electrical properties of the channel whereas those introducing rings greatly weaken amiloride block, we conclude that amiloride binds at or near this site and that alphaSer-583 may have a role in ion permeation through ENaC.
authors.affiliation: Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Ossama B
authors.initials: OB
authors.lastname: Kashlan
authors.forename: Shaohu
authors.initials: S
authors.lastname: Sheng
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Kleyman
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D004232:Diuretics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D053503:Epithelial Sodium Channels; D005998:Glycine / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011485:Protein Binding; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D018075:RNA, Complementary / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D015222:Sodium Channels / Q000737:chemistry*; D014982:Xenopus laevis
chemical_list: D004232:Diuretics; D053503:Epithelial Sodium Channels; D007477:Ions; D018075:RNA, Complementary; D015222:Sodium Channels; C013407:benzamil; D012694:Serine; D006639:Histidine; D000584:Amiloride; D012964:Sodium; D005998:Glycine