Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity.
The neuropeptide galanin (GAL) is involved in food intake and in fat ingestion. Presumably, these effects are conveyed via the galanin 1 receptor (GALR1). We screened the coding region of GAL (including 444 bp of its promoter region) and GALR1 for mutations using single-strand conformation polymorphism analysis and denaturing HPLC in up to 191 obese children and adolescents and 106 healthy underweight young adults (students). In GAL, we identified 3 novel single nucleotide polymorphisms (SNPs; silent: g.-419T-->C, g.-244G-->A; missense: g.47C-->T: Ala16Val) and one infrequent missense variation (c.253A-->G: Asn85Asp), and in GALR1 2 novel SNPs (silent: c.150C-->T, missense: c.793A-->T: Ile265Phe). To test for an association with obesity, we genotyped 7 SNPs (GAL: g.-244G-->A, g.47C-->T, rs7101947, rs1042577, rs3136540; GALR1: c.150C-->T, c.793A-->T) in up to 322 obese children and adolescents compared with up to 277 healthy underweight and normal weight young adults. Furthermore, we analyzed these SNPs with respect to potential effects on the percentage of energy consumed as fat in obese children and adolescents. Allele and genotype frequencies did not differ among the groups tested. In addition, we performed a pedigree transmission disequilibrium test (PDT) for one SNP (GAL: g.-244G-->A) in 610 (518 independent) obesity-trios (obese child or adolescent and both of its parents). However, the PDT for SNP GAL g.-244G-->A revealed no transmission disequilibrium. We conclude that the analyzed SNPs in GAL and GALR1 do not play a major role in early onset obesity or dietary fat intake in the obese children and adolescents of our study groups.
Schäuble
Nadine
N
Clinical Research Group, Department of Child and Adolescent Psychiatry, Philipps-University of Marburg, Germany.
Reichwald
Kathrin
K
Grassl
Wolfgang
W
Bechstein
Helen
H
Müller
Hans-Christian
HC
Scherag
André
A
Geller
Frank
F
Utting
Michael
M
Siegfried
Wolfgang
W
Goldschmidt
Hanspeter
H
Blundell
John
J
Lawton
Clare
C
Alam
Rahul
R
Whybrow
Stephen
S
Stubbs
James
J
Platzer
Matthias
M
Hebebrand
Johannes
J
Hinney
Anke
A
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D004041:Dietary Fats / Q000008:administration & dosage*; D004435:Eating / Q000235:genetics / Q000502:physiology*; D005260:Female; D019004:Galanin / Q000235:genetics / Q000502:physiology*; D014644:Genetic Variation; D006801:Humans; D008297:Male; D009765:Obesity / Q000209:etiology*; D020641:Polymorphism, Single Nucleotide; D044091:Receptor, Galanin, Type 1 / Q000235:genetics / Q000502:physiology*
D004041:Dietary Fats; D044091:Receptor, Galanin, Type 1; D019004:Galanin