A practical molecular clip for immobilization of receptors and biomolecules on devices' surface: synthesis, grafting protocol and analytical assay.
Deposition of O-succinimidyl 4-(p-azido-phenyl)butanoate (4) onto inorganic device (FTIR-ATR crystal) or polymer material (filtration membrane) followed by irradiation at 254nm led to surface functionalisation with NHS esters. Further reaction with biomolecules allowed their covalent grafting. The reactivity of the photoactivated surfaces was assayed by two methods: (i) the coupling with 3,5-bis(trifluoromethyl)benzylamine (7) and subsequent XPS analysis; (ii) the coupling with 4,5-bis-tritiated lysine (10) and subsequent LSC measurement of the radioactivity.
Devouge
Sabrina
S
Université Catholique de Louvain, Unité de Chimie Organique et Médicinale, Bâtiment Lavoisier, Place L. Pasteur No. 1, B-1348 Louvain-la-Neuve, Belgium.
Salvagnini
Claudio
C
Marchand-Brynaert
Jacqueline
J
D000925:Anticoagulants / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D010777:Photochemistry; D013388:Succinimides / Q000138:chemical synthesis*; D013499:Surface Properties; D013917:Thrombin / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D003432:Cross-Linking Reagents; D013388:Succinimides; D013917:Thrombin; C001426:N-hydroxysuccinimide