title: [Proteomic analysis on metastasis-associated proteins of hepatocellular carcinoma tissues].
abstract: OBJECTIVE
  A comparative proteomic approach was used to identify and analyze proteins relevant to metastasis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  Proteins extracted from 12 liver tumor tissue specimens (6 with metastases and 6 without) were separated by two-dimensional gel electrophoresis (2-DE). Comparative analyses of 2-DE protein patterns between the two groups were done using computerized image analysis. Selected proteins exhibiting statistically significant alternations were identified by mass spectrometry. Immunohistochemistry, Western blotting and RT-PCR were performed to examine the expressions of the candidate proteins.
  
  
  RESULTS
  16 proteins including HSP27, S100A11, CK18 were identified using mass spectrometry, which were related to cell mobility, signal transduction, and energy metabolism respectively. Of these, HSP27 was found to be uniquely over-expressed in 2-DE maps of all metastatic HCCs when compared to the non-metastatic HCC tissues. Immunohistochemistry and Western blotting of HCC tissues confirmed this difference while RT-PCR did not.
  
  
  CONCLUSIONS
  There are different proteins working together that affect the metastasis of HCCs. The overexpression of HSP27 may serve as a biomarker for early detection and therapeutic targets to the metastatic phenotype of HCC. The role of HSP27 in HCC metastasis warrants further investigation.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Song
authors.forename: Yin-kun
authors.initials: YK
authors.lastname: Liu
authors.forename: Jie-feng
authors.initials: JF
authors.lastname: Cui
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dai
authors.forename: Ju-tao
authors.initials: JT
authors.lastname: Feng
authors.forename: Jiu-xian
authors.initials: JX
authors.lastname: Feng
authors.forename: Hua-li
authors.initials: HL
authors.lastname: Shen
authors.forename: Peng-yuan
authors.initials: PY
authors.lastname: Yang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D013058:Mass Spectrometry; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020543:Proteome; D009418:S100 Proteins; C072337:S100A11 protein, human