Molecular characterization of a Phi-class mustard (Brassica juncea) glutathione S-transferase gene in Arabidopsis thaliana by 5'-deletion analysis of its promoter.
Glutathione S-transferases (GSTs) are regulated by various stimuli at the transcriptional level. In this study, a 2,640-bp promoter sequence of a mustard GST gene, BjGSTF2, was cloned. Several truncated BjGSTF2 promoters were generated by 5'-deletion, fused to the beta-glucuronidase (GUS) coding sequence and the chimeric genes expressed in Arabidopsis thaliana. Transgene expression in GST2623::GUS plants carrying the longest promoter varied considerably. GUS activity was high in the roots, cotyledons, anthers and both ends of the silique, but it was low or barely detectable in the leaves, seeds, petals and stamens. Analysis of transgenic plants expressing the GUS gene under the control of different truncated BjGSTF2 promoters revealed several regions that possessed cis-acting elements required for the basal and induced expression by H(2)O(2), salicylic acid and 1-aminocyclopropane-1-carboxylate and down-regulation by spermidine. The results also showed that the GUS activity of GST2623::GUS coincided well with the H(2)O(2) accumulation pattern in cultured leaf-disc explants during the regeneration process.
Gong
Haibiao
H
Plant Genetic Engineering Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of Singapore.
Hu
Wen-Wei
WW
Jiao
Yuxia
Y
Pua
Eng-Chong
EC
D000598:Amino Acids, Cyclic / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004789:Enzyme Activation / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000201:enzymology* / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012038:Regeneration / Q000187:drug effects / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology
D000598:Amino Acids, Cyclic; C023863:1-aminocyclopropane-1-carboxylic acid; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase; D020156:Salicylic Acid; D013095:Spermidine