[Observation and analysis on mutation of routine STR locus].
OBJECTIVE
To observe and analyze the characteristic of mutation at STR locus.


METHODS
27 mutant genes observed in 1211 paternity testing cases were checked by PAGE-silver stained and PowerPlex 16 System Kit and validated by sequencing.


RESULTS
Mutant genes locate on 15 loci. The pattern of mutation was accord with stepwise mutation model. The mutation ratio of male-to-female was 8:1 and correlated to the age of father.


CONCLUSIONS
Mutation rate is correlated to the geometric mean of the number of homogeneous repeats of locus. The higher the mean, the higher the mutation rate. These loci are not so appropriate for use in paternity testing.
Li
Qiu-yang
QY
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. xwqy1111@hotmail.com
Feng
Wei-jun
WJ
Yang
Qin-gen
QG
D000367:Age Factors; D000483:Alleles; D004247:DNA / Q000097:blood; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D010334:Paternity; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012737:Sex Factors; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004247:DNA