A simple and inexpensive light source for research in visual neuroscience.
Investigating the properties of light responsive neurons and their networks requires appropriate control of stimulus parameters, such as intensity, spectral composition, spatial and temporal profile. In the present paper, we describe how to build a simple, versatile and low-cost light source for use in visual neuroscience. The light source is a InGaN-based ultrabright light-emitting diode (LED), which may generate conventional light flashes as well as a variety of time varying stimuli to be used in quantitative studies of the visual system. In particular, with this instrument one may generate light stimuli sinusoidally modulated in time at frequencies ranging from 0.05 to 50 Hz, with less than 1% harmonic distortion at a contrast exceeding 85%. The relationship between applied voltage and energy emitted by the source is linear over an intensity range that exceeds 4.5 log-units, up to the full suppression of the light-sensitive currents in mammalian rods. The light source has minimal space requirement and does not generate appreciable radiating heat and hum, allowing its use for single cell work "in vitro" as well as for "in vivo" recording of the electroretinogram (ERG).
Demontis
Gian Carlo
GC
Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Università di Pisa, Via Bonanno 6, I-56126 Pisa, Italy. demontis@farm.unipi.it
Sbrana
Andrea
A
Gargini
Claudia
C
Cervetto
Luigi
L
D000818:Animals; D002478:Cells, Cultured; D004581:Electronics / Q000295:instrumentation / Q000379:methods; D004596:Electroretinography / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D005583:Fourier Analysis; D006168:Guinea Pigs; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D008297:Male; D008564:Membrane Potentials / Q000502:physiology / Q000528:radiation effects; D009482:Neurophysiology / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D010775:Photic Stimulation / Q000295:instrumentation* / Q000379:methods; D012160:Retina / Q000502:physiology*; D017948:Retinal Rod Photoreceptor Cells / Q000502:physiology / Q000528:radiation effects; D014785:Vision, Ocular / Q000502:physiology / Q000528:radiation effects