Profile of enzyme production by Trichoderma reesei grown on corn fiber fractions.
Corn fiber is the fibrous by-product of wet-mill corn processing. It typically consists of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. Crude corn fiber (CCF) was fractionated into de-starched corn fiber (DSCF), corn fiber with cellulose (CFC) enriched, and corn fiber arabinoxylan (CFAX), and these fractions were evaluated as substrates for enzyme production by Trichoderma reesei. T. reesei QM9414 and Rut C-30 grew on CCF, DSCF, CFC, or CFAX and secreted a number of hydrolytic enzymes. The enzymes displayed synergism with commercial cellulases for corn fiber hydrolysis.
Li
Xin-Liang
XL
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 N. University Street, Peoria, Illinois 61604. lix@ncaur.usda.gov
Dien
Bruce S
BS
Cotta
Michael A
MA
Wu
Y Victor
YV
Saha
Badal C
BC
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D044602:Cellulases / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
D010936:Plant Extracts; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases