The response of Na+/K+ -ATPase of human erythrocytes to green laser light treatment.
The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na(+)/K(+)-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na(+)/K(+)-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R(2)=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm(-2)). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.
Kassák
P
P
Division of Biomedical Physics, Faculty of Mathematics, Physics, and Computer Science, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovak Republic. pkassak@yahoo.com
Sikurová
L
L
Kvasnicka
P
P
Bryszewska
M
M
D003116:Color; D004912:Erythrocytes / Q000528:radiation effects*; D005456:Fluorescent Dyes; D006801:Humans; D007834:Lasers; D028022:Low-Level Light Therapy; D011744:Pyrimidinones; D000254:Sodium-Potassium-Exchanging ATPase / Q000528:radiation effects*
D005456:Fluorescent Dyes; D011744:Pyrimidinones; C003954:merocyanine dye; D000254:Sodium-Potassium-Exchanging ATPase