Detection of fragmented genomic DNA by PCR-free piezoelectric sensing using a denaturation approach.
Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.
Minunni
Maria
M
Dipartimento di Chimica, Università degli Studi di Firenze, 50019 Sesto Fiorentino, Italy. minunni@unifi.it
Tombelli
Sara
S
Fonti
Jessica
J
Spiriti
Maria M
MM
Mascini
Marco
M
Bogani
Patrizia
P
Buiatti
Marcello
M
D015374:Biosensing Techniques / Q000379:methods; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D014026:Nicotiana / Q000235:genetics
D018744:DNA, Plant; D004277:DNA, Single-Stranded; D009841:Oligonucleotides