title: Sequence analysis of near full-length HIV type 1 subtype D primary strains isolated in Cape Town, South Africa, from 1984 to 1986.
abstract: South Africa has one of the fastest growing HIV-1 epidemics worldwide, consisting mostly of subtype C. However, HIV-1 subtype B and subtype D viruses were isolated in the beginning of the epidemic in the early 1980s. This study describes the amplification, cloning, and near full-length genome sequencing of four HIV-1 subtype D primary strains, isolated from 1984 to 1986 in Cape Town, in what seems to have been a small restricted subtype D epidemic in the country.
authors.affiliation: Department of Medical Virology, National Health Laboratory Service (NHLS) and University of Stellenbosch, Tygerberg Academic Hospital, Tygerberg, 7505, South Africa.
authors.forename: André G
authors.initials: AG
authors.lastname: Loxton
authors.forename: Florette
authors.initials: F
authors.lastname: Treurnicht
authors.forename: Annette
authors.initials: A
authors.lastname: Laten
authors.forename: Estrelita Janse
authors.initials: EJ
authors.lastname: van Rensburg
authors.forename: Susan
authors.initials: S
authors.lastname: Engelbrecht
mesh_terms: D004196:Disease Outbreaks; D016679:Genome, Viral; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013019:South Africa / Q000453:epidemiology