title: [The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus].
abstract: OBJECTIVE
  To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients.
  
  
  METHODS
  Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA.
  
  
  RESULTS
  In 162 serum samples, 90.2% (55/61) became N protein positive in 1 - 5 days and 92.8% (13/14) became positive IgM and IgG in 15 - 18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3% (14/24) in 1 - 5 days and 71.4% (10/14) in 6 - 9 days after the onset.
  
  
  CONCLUSIONS
  Other than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.
authors.affiliation: Guangzhou Centre of Disease Control and Prevention, Guangzhou 510080, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Duan-hua
authors.initials: DH
authors.lastname: Zhou
authors.forename: Xin-wei
authors.initials: XW
authors.lastname: Wu
authors.forename: Xiao-shuang
authors.initials: XS
authors.lastname: Chen
authors.forename: Biao
authors.initials: B
authors.lastname: Di
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Du
authors.forename: Hui-fang
authors.initials: HF
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Gu
authors.forename: Bo-jian
authors.initials: BJ
authors.lastname: Zheng
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Xu
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D019590:Nucleocapsid Proteins / Q000097:blood; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic