title: Nerve-pathways of acupoint Fengch'ih in rat by anterograde transport of HRP.
abstract: OBJECTIVE
  To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP).
  
  
  METHODS
  Fifty Wistar rats were randomly divided into 1, 2, 3, 4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih. Serial, transverse or capital, 40 microm sections of the cervical spinal ganglia, cervical and thoracic spinal cord segment and brain were cut on a cryotome. Sections were incubated for HRP histochemistry according to the tetramethylbenzidine (TMB). Part of the sections were counterstained with neutral red.
  
  
  RESULTS
  After 1 d of survival times, many labeled cell bodies were found in 1-4 cervical spinal ganglia, anterior horn of 1-4 cervical spinal cord, ventromedial division of facial nucleus, accessory facial nucleus ipsilaterally. With increasing survival times, the intensity of labeled cells were slightly decreased.
  
  
  CONCLUSIONS
  Fengch'ih may bring into full play its effect by correlation of posterior ear branch of facial nerve and anterior branch of 2-3 cervical nerve with 1-4 cervical the anterior horn of the spinal cord, ventromedial division of facial nucleus, accessory facial nucleus.
authors.affiliation: Department of Neurology, Shiyan Renmin Hospital Affiliated to Yunyang Medical College, Shiyan, Hubei Province, China.
authors.forename: Gang-Ming
authors.initials: GM
authors.lastname: Xi
authors.forename: Hua-Qiao
authors.initials: HQ
authors.lastname: Wang
authors.forename: Guo-Hou
authors.initials: GH
authors.lastname: He
authors.forename: Chao-Feng
authors.initials: CF
authors.lastname: Huang
authors.forename: Qun-Fang
authors.initials: QF
authors.lastname: Yuan
authors.forename: Guo-Yao
authors.initials: GY
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Liu
authors.forename: Hua-Yan
authors.initials: HY
authors.lastname: Fan
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D009434:Neural Pathways / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions
chemical_list: D012996:Solutions; D006735:Horseradish Peroxidase