Development of Isospora belli in Hct-8, Hep-2, human fibroblast, BEK and Vero culture cells.
The development of Isospora belli, a human coccidian parasite, was studied in different cell lines. Merozoites were observed in all kinds of cells, whereas sporogony was demonstrated only in Hct-8. This implied that not only the human cell line can be infected, but also some animal cell lines. Unizoites could be found in Vero cells. The merozoites were transferred to a new culture cell for three passages and maintained for two weeks, but no oocyst production was observed in any culture cells during cultivation.
Siripanth
Chutatip
C
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmcsr@mahidol.ac.th
Punpoowong
Benjanee
B
Amarapal
Pornsawan
P
Thima
Niramol
N
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005243:Feces / Q000469:parasitology; D005347:Fibroblasts / Q000469:parasitology; D006790:Host-Parasite Interactions; D006801:Humans; D007549:Isospora / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D013045:Species Specificity; D034101:Sporozoites / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014709:Vero Cells