title: 36-kDa microfibril-associated glycoprotein (MAGP-36) is an elastin-binding protein increased in chick aortae during development and growth.
abstract: MAGP-36 was discovered in porcine aorta in 1989 and is thought to be one of the microfibril-associated proteins. MAGP-36 has been localized on the surface of elastic fibers or laminae in immunohistochemical studies. However, its functional role in the aorta is obscure. Herein, we report on the binding activity of MAGP-36 to components of the aortic wall and its accumulation pattern in the aorta during development and growth. In vitro, MAGP-36 bound to elastin and collagen in a Ca(2+)-dependent manner, and mediated the adhesion of human aortic smooth muscle cells. This cell adhesion mostly depended on the RGD-containing domain of MAGP-36. We examined the accumulation of MAGP-36 with quantitative Western blot analysis and immunoelectron microscopy in chick aortae during development and growth. The amount of MAGP-36 increased on the surface of elastic fibers or laminae between days 14 and 34 after the start of incubation, and reached a plateau at about 53 days. This accumulation of MAGP-36 roughly correlated with an increase in blood pressure for this period. Thus, MAGP-36 might be a bridging protein that connects elastin to other components of the aortic wall and might play a role in maintaining the integrity of the aortic structure under arterial pressure.
authors.affiliation: Department of Structure and Functional Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan. tetsu@kms.ac.jp
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Toyoshima
authors.forename: Nozomu
authors.initials: N
authors.lastname: Nishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kusama
authors.forename: Ryoji
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Itano
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D015153:Blotting, Western; D002448:Cell Adhesion; D002478:Cells, Cultured; D002642:Chick Embryo; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D020894:Microfibrils / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D006023:Glycoproteins; D011956:Receptors, Cell Surface; C054901:elastin-binding proteins