title: The ATP levels in kidneys and blood are mainly decreased by acute ingestion of tullidora (Karwinskia humboldtiana).
abstract: Our previous acute toxicity studies with Karwinskia humboldtiana (Kh) in rats showed renal hemodynamic changes with a marked increase in the fractional excretion of sodium and morphological damage. To analyse the effects of Kh or 'tullidora' on energetic metabolism, a single dose of an oral preparation from the seed fruits was given to Wistar rats (1.25 g/kg). In tullidora-treated rats there was 8% mortality. ATP concentrations in renal tissue decreased significantly (control: 53.85+/-3.34, tullidora 38.28+/-5.31 micromol/g fresh tissue, P<0.05). Total blood (54.8+/-0.96, tullidora: 40.2+/-1.55 micromol/dL, P<0.01) and haemoglobin-ATP concentrations (3.69+/-0.12, tullidora: 2.56+/-0.11 micromol/g, P<0.01) were also significantly diminished. Moreover, the total protein in renal cortex from tullidora-treated rats decreased as compared to control group (control: 71.43+/-2.88, tullidora: 55.20+/-4.06 mg/g fresh tissue, P<0.05). In contrast, Na+-K+-ATPase activity in tullidora-treated animals was not different from control rats. These findings might partially explain the acute effects and mortality observed in the Kh treated rats.
authors.affiliation: Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Avenida Universidad 940, Fraccionamiento Los Bosques, CP 20100 Aguascalientes, Ags., Mexico. jara@att.net.mx
authors.forename: F
authors.initials: F
authors.lastname: Jaramillo-Juárez
authors.forename: M L
authors.initials: ML
authors.lastname: Rodríguez-Vázquez
authors.forename: J
authors.initials: J
authors.lastname: Muñoz-Martínez
authors.forename: T
authors.initials: T
authors.lastname: Quezada-Tristán
authors.forename: F A
authors.initials: FA
authors.lastname: Posadas del Río
authors.forename: J
authors.initials: J
authors.lastname: Llamas-Viramontes
authors.forename: G Gabriel
authors.initials: GG
authors.lastname: Ortíz
authors.forename: Alfredo
authors.initials: A
authors.lastname: Feria-Velasco
authors.forename: J L
authors.initials: JL
authors.lastname: Reyes
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood* / Q000378:metabolism*; D000818:Animals; D031956:Karwinskia / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D007928:Lethal Dose 50; D008297:Male; D010947:Plants, Toxic; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000255:Adenosine Triphosphate