Identification of differentially expressed proteins during human urinary bladder cancer progression.
Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss of IDPc and Prx-II in more advance tumor samples, in agreement with data on cell lines. These results suggest that loss of IDPc and Prx-II during tumor development may involve in tumor progression and metastasis. However, additional investigations are needed on large number of human samples to further verify these findings.
Memon
Ashfaque A
AA
Department of Life Science, Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea. amemo@akh.aaa.dk
Chang
Jong W
JW
Oh
Bong R
BR
Yoo
Yung J
YJ
D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D015536:Down-Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D009367:Neoplasm Staging; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D054464:Peroxiredoxins; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
D006360:Heat-Shock Proteins; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D010544:Peroxidases; C086065:PRDX2 protein, human; D054464:Peroxiredoxins