Gene transfer to dorsal root ganglia by intrathecal injection: effects on regeneration of peripheral nerves.
Gene delivery to sensory neurons of the dorsal root ganglion (DRG) offers the prospect of developing new clinical interventions against peripheral nerve diseases and disorders. Here we show that genes can be transferred to rat DRG through lumbar intrathecal injection of delivery vectors into the cerebrospinal fluid. Genes could be transferred to DRG using polyethylenimine (PEI)/DNA complexes, Lipofectamine 2000/DNA complexes, adeno-associated virus vectors, or baculovirus vectors. We also show that nerve growth factor cDNA, delivered through lumbar intrathecal injection of PEI complexes, was able to improve regeneration of transected rat sciatic nerves. These data demonstrate the viability of using an intrathecal gene delivery approach for treating peripheral neuropathies.
Wang
Xu
X
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos No. 04-41, Singapore 138669.
Wang
Chaoyang
C
Zeng
Jieming
J
Xu
Xiaoyun
X
Hwang
Peter Y K
PY
Yee
Woon-Chee
WC
Ng
Yee-Kong
YK
Wang
Shu
S
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D018076:DNA, Complementary / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics; D005727:Ganglia, Spinal / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D007278:Injections, Spinal; D008055:Lipids / Q000235:genetics; D008081:Liposomes; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D009414:Nerve Growth Factors; D009416:Nerve Regeneration / Q000235:genetics; D011094:Polyethyleneimine; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000502:physiology*
D018076:DNA, Complementary; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D009414:Nerve Growth Factors; D011094:Polyethyleneimine; D008156:Luciferases