title: An unusual primate locus that attracted two independent Alu insertions and facilitates their transcription.
abstract: BC200 RNA, a neuronal, small non-messenger RNA that originated from a monomeric Alu element is specific to anthropoid primates. Tarsiers lack an insert at the orthologous genomic position, whereas strepsirrhines (Lemuriformes and Lorisiformes) acquired a dimeric Alu element, independently from anthropoids. In Galago moholi, the CpG dinucleotides are conspicuously conserved, while in Eulemur coronatus a large proportion is changed, indicating that the G.moholi Alu is under purifying selection and might be transcribed. Indeed, Northern blot analysis of total brain RNA from G.moholi with a specific probe revealed a prominent signal. In contrast, a corresponding signal was absent from brain RNA from E.coronatus. Isolation and sequence analysis of additional strepsirrhine loci confirmed the differential sequence conservation including CpG patterns of the orthologous dimeric Alu elements in Lorisiformes and Lemuriformes. Interestingly, all examined Alu elements from Lorisiformes were transcribed, while all from Lemuriformes were silent when transiently transfected into HeLa cells. Upstream sequences, especially those between the transcriptional start site and -22 upstream, were important for basal transcriptional activity. Thus, the BC200 RNA gene locus attracted two independent Alu insertions during its evolutionary history and provided upstream promoter elements required for their transcription.
authors.affiliation: Institute of Experimental Pathology, ZMBE, University of Münster, Von-Esmarch-Str. 56, D-48149 Münster, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ludwig
authors.forename: T S
authors.initials: TS
authors.lastname: Rozhdestvensky
authors.forename: V Yu
authors.initials: VY
authors.lastname: Kuryshev
authors.forename: J
authors.initials: J
authors.lastname: Schmitz
authors.forename: J
authors.initials: J
authors.lastname: Brosius
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D020087:Alu Elements / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D018899:CpG Islands / Q000235:genetics; D005701:Galago / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010802:Phylogeny; D011323:Primates / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D012333:RNA, Messenger