title: Importance of eIF2alpha phosphorylation and stress granule assembly in alphavirus translation regulation.
abstract: Alphavirus infection results in the shutoff of host protein synthesis in favor of viral translation. Here, we show that during Semliki Forest virus (SFV) infection, the translation inhibition is largely due to the activation of the cellular stress response via phosphorylation of eukaryotic translation initiation factor 2alpha subunit (eIF2alpha). Infection of mouse embryo fibroblasts (MEFs) expressing a nonphosphorylatable mutant of eIF2alpha does not result in efficient shutoff, despite efficient viral protein production. Furthermore, we show that the SFV translation enhancer element counteracts the translation inhibition imposed by eIF2alpha phosphorylation. In wild-type MEFs, viral infection induces the transient formation of stress granules (SGs) containing the cellular TIA-1/R proteins. These SGs are disassembled in the vicinity of viral RNA replication, synchronously with the switch from cellular to viral gene expression. We propose that phosphorylation of eIF2alpha and the consequent SG assembly is important for shutoff to occur and that the localized SG disassembly and the presence of the enhancer aid the SFV mRNAs to elude general translational arrest.
authors.affiliation: Microbiology and Tumour Biology Centre, Karolinska Institute, Stockholm 171 77, Sweden. gerald.mcinerney@mtc.ki.se
authors.forename: Gerald M
authors.initials: GM
authors.lastname: McInerney
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Kedersha
authors.forename: Randal J
authors.initials: RJ
authors.lastname: Kaufman
authors.forename: Paul
authors.initials: P
authors.lastname: Anderson
authors.forename: Peter
authors.initials: P
authors.lastname: Liljeström
mesh_terms: D000524:Alphavirus / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D051379:Mice; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D015852:Eukaryotic Initiation Factor-2; D012367:RNA, Viral; D010768:Phosphoserine