title: Detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness.
abstract: BACKGROUND
  Reverse transcription polymerase chain reaction (RT-PCR) is a powerful tool that allows the detection of minute quantities of viral RNA. Because of the sensitivity of these assays it is possible that the finding of viral RNA indicates not only active infection but also transient colonization or residual nucleic acid from a distant infection. Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two viruses for which RT-PCR is now frequently used for diagnosis in adult disease.
  
  
  OBJECTIVE
  We evaluated nasal secretions from adults with and without respiratory illnesses by nested, one-tube RT-PCR for RSV and hMPV to determine if rates of detectable RNA were significantly higher among ill subjects compared to controls suggesting a causal relationship with respiratory illness.
  
  
  METHODS
  Adults presenting to a health care provider with complaints of respiratory illness were recruited as "cases" and those visiting for non-respiratory complaints were recruited as "controls". Subjects were enrolled during a 3-month period (January to April) when both viruses were expected to be prevalent in the community. Nasal swab samples were obtained and subjected to one-tube nested RT-PCR for RSV and hMPV.
  
  
  RESULTS
  Of 146 ill subjects, 17 (11.6%) tested positive for RSV and 5 (3.4%) were positive for hMPV. Of the 158 control subjects, one was RT-PCR positive for RSV and none tested positive for hMPV. The rates of RT-PCR positive cases compared to controls were significantly different for RSV (p<.0001) and hMPV (p<.02). Subjects remained RSV RT-PCR positive on average until day 7.1+/-2.8 of symptoms with a range of 3-10 days. No subject had a positive swab on days 14, 21 or 28.
  
  
  CONCLUSIONS
  Asymptomatic carriage of RSV or hMPV is uncommon. RT-PCR should be a useful method for the diagnosis of these viral illnesses in adults.
authors.affiliation: Department of Medicine, Infectious Diseases Unit, Rochester General Hospital, 1425 Portland Avenue, Rochester, NY 14621, USA.
authors.forename: Ann R
authors.initials: AR
authors.lastname: Falsey
authors.forename: Mary C
authors.initials: MC
authors.lastname: Criddle
authors.forename: Edward E
authors.initials: EE
authors.lastname: Walsh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D009666:Nose / Q000821:virology; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000503:physiopathology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods
chemical_list: D012367:RNA, Viral