[Effect of blood purification materials and anticoagulants on the contact activity of factor XI].
OBJECTIVE
This study was conducted to examine the degree of contact activation of five blood purification materials sulfonated polyethersulfone (PES-SO3Na), polyethersulfone (PES), polysulfone (PS), polymethylmethacrylate (PMMA) and cellulose acetate (CA) after they were incubated in anticoagulant-containing human plasma for 10 minutes at 37 degrees C.


METHODS
Three anticoagulants Heparin, Trisodium citrate and Disodium EDTA were used. The time course for generation of FXII activity by the materials after incubation in anticoagulant-containing human plasma was evaluated. The degree of contact activation was determined by measuring membrane-bound alpha-FXIIa activity level.


RESULTS
(1) The five materials caused different increase at different rate in the generation of alpha-FXIIa after they were separately incubated with each of the three anticoagulants. (2) PES-SO3Na activated the least FXII no matter which anticoagulant the plasma contained.


CONCLUSIONS
Contact activation can be mediated by antithrombotic agent. PES-SO3Na has the best blood compatibility with respect to contact activation considering intrinsic coagulation.
Cheng
Li-ping
LP
Center for Biomedical Engineering, College of Material Science and Engineering, Sichuan Univeisity, Chengdu 610065, China.
Sun
Shu-dong
SD
Yue
Yi-lun
YL
Huang
Jia
J
D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D002951:Citrates / Q000737:chemistry / Q000494:pharmacology; D005174:Factor XII / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D006435:Renal Dialysis / Q000295:instrumentation*; D000077559:Sodium Citrate; D013450:Sulfones / Q000737:chemistry / Q000494:pharmacology*
D000925:Anticoagulants; D001672:Biocompatible Materials; D002951:Citrates; D005343:Fibrinolytic Agents; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D000077559:Sodium Citrate; C017662:polysulfone P 1700; C022840:polyether sulfone; C005062:acetylcellulose; D005174:Factor XII; D002482:Cellulose; D006493:Heparin