title: Oligomeric proteins ultrastructurally localize to cell processes, especially to axon terminals with higher density, but not to lipid rafts in Tg2576 mouse brain.
abstract: We examined the ultrastructural localization of oligomeric proteins, Abeta42, and flotillin-1 in Tg2576 mouse brains by triple immunoelectron microscopy. Oligomer-specific immunoreactions localized to cell processes, especially to axon terminals with higher density in Tg than in nonTg mouse brains. The oligomer was less frequently colocalized to flotillin-1-immunoreactive rafts than Abeta42, suggesting that rafts are one of the sites of polymeric Abeta deposition, but not of oligomeric proteins including Abeta.
authors.affiliation: Gunma University School of Health Sciences, 3-39-15 Showa-machi, Maebashi 371-8514, Japan.
authors.forename: Hideko
authors.initials: H
authors.lastname: Kokubo
authors.forename: Rakez
authors.initials: R
authors.lastname: Kayed
authors.forename: Charles G
authors.initials: CG
authors.lastname: Glabe
authors.forename: Takaomi C
authors.initials: TC
authors.lastname: Saido
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Iwata
authors.forename: J Bernd
authors.initials: JB
authors.lastname: Helms
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Yamaguchi
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D004195:Disease Models, Animal; D021962:Membrane Microdomains / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D016253:Microscopy, Immunoelectron; D010446:Peptide Fragments / Q000378:metabolism*; D058225:Plaque, Amyloid / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); C106354:flotillins