title: Interaction of the protein phosphatase 2A with the regulatory domain of the cystic fibrosis transmembrane conductance regulator channel.
abstract: A direct interaction of the regulatory domain (R domain) of the cystic fibrosis transmembrane conductance regulator protein (CFTR) with PR65, a regulatory subunit of the protein phosphatase 2A (PP2A), was shown in yeast two hybrid, pull-down and co-immunoprecipitation experiments. The R domain could be dephosphorylated by PP2A in vitro. Overexpression of the interacting domain of PR65 in Caco-2 cells, as well as treatment with okadaic acid, showed a prolonged deactivation of the chloride channel. Taken together our results show a direct and functional interaction between CFTR and PP2A.
authors.affiliation: Department of Human Genetics, Division of Human Mutations and Polymorphisms, KULeuven, Herestraat 49, Postbus 602, 3000 Leuven, Belgium.
authors.forename: Annick
authors.initials: A
authors.lastname: Vastiau
authors.forename: Lishuang
authors.initials: L
authors.lastname: Cao
authors.forename: Martine
authors.initials: M
authors.lastname: Jaspers
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Owsianik
authors.forename: Veerle
authors.initials: V
authors.lastname: Janssens
authors.forename: Harry
authors.initials: H
authors.lastname: Cuppens
authors.forename: Jozef
authors.initials: J
authors.lastname: Goris
authors.forename: Bernd
authors.initials: B
authors.lastname: Nilius
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Cassiman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018938:Caco-2 Cells; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D054648:Protein Phosphatase 2; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C505032:CFTR protein, human; D021122:Protein Subunits; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2