Transcription past DNA adducts derived from polycyclic aromatic hydrocarbons.
The ability of a DNA lesion to block transcription is a function of many variables: (1) the ability of the RNA polymerase active site to accommodate the damaged base; (2) the size and shape of the adduct, which includes the specific modified base; (3) the stereochemistry of the adduct; (4) the base incorporated into the growing transcript; (5) and the local DNA sequence. Each of these parameters, either alone or in combination, can influence how a particular lesion in the genome will affect transcription elongation, resulting in potential clearance of the lesion via transcription-coupled DNA repair or in the formation of truncated or full-length transcripts that might encode defective proteins.
Scicchitano
David A
DA
Department of Biology, 1009 Silver Center, 100 Washington Square East, New York University, New York, NY 10003, USA. das2@nyu.edu
D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry*; D014158:Transcription, Genetic
D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct; D004247:DNA