[Effects of Chinese herb compound on myocardial SDH, ATP-ase and energy reserves in tail-suspended rats].
OBJECTIVE
To investigate effects of Chinese herb compound on myocardial SDH, ATP-ase and energy reserves in tail-suspended rats.


METHODS
Male SD rats were randomly divided into three groups (n=10 each): (A) normal control group; (B) tail-suspended group; (C) tail-suspended + Chinese Medical herb compound group. Rats in group B and C were tail-suspended (-30 degrees) for 5 d to simulate weightlessness. All rats B were decapitated after the experiment. Myocardial SDH (method of Anderson N-BT), ATP-ase (method of Wachstein and Meisel) and energy reserves (method of HPLC) were examined.


RESULTS
Compared with rats in groups A and C, SDH activity increased, SDH staining deepened, value of OD SDH enhanced, ATP-ase activity and OD value increased and enhanced significantly (P<0.01) but value of myocardial energy reserves decreased in group B rats.


CONCLUSIONS
Tail-suspension strengthens compensatory oxidation metabolism of cardiac muscle and activates SDH and ATP-ase, at the same time decrease, myocardial energy reserves. Normal are maintained the low level of metabolism by the medical herb compound used which shows a protecting effects through mitigation, tranguilization and replenishment of Qi.
Wang
Bao-zhen
BZ
Institute of Space Medico-Engineering, Beijing, China.
Li
Yong-zhi
YZ
Xin
Bing-mu
BM
Fan
Quan-chun
QC
Bai
Gui-e
GE
D000251:Adenosine Triphosphatases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D019417:Hindlimb Suspension; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000187:drug effects* / Q000378:metabolism; D018474:Weightlessness Simulation
D004365:Drugs, Chinese Herbal; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases