title: Indole alkaloids from Tabernaemontana australis (Muell. Arg) Miers that inhibit acetylcholinesterase enzyme.
abstract: Ten indole alkaloids from the chloroform extract of stalk of Tabernaemontana australis (Muell. Arg) Miers were tentatively identified by GC-MS, viz., coronaridine (1), voacangine (2), voacangine hydroxyindolenine (3), rupicoline (4), ibogamine (5), ibogaine (6), ibogaline (7), desethyl-voacangine (8), voachalotine (9), and affinisine (10). Of these, the first four were isolated by silica gel open column chromatography, identified by uni- and bidimensional NMR, IR, MS and showed anti-cholinesterasic activity at the same concentration as the reference compounds physostigmine and galanthamine (detection limit of 0.01mM) by TLC assay using the modified Ellman's method.
authors.affiliation: Instituto de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco A, Cidade Universitária, 21945-970 Rio de Janeiro, RJ, Brazil.
authors.forename: Marcelo T
authors.initials: MT
authors.lastname: Andrade
authors.forename: Josélia A
authors.initials: JA
authors.lastname: Lima
authors.forename: Angelo C
authors.initials: AC
authors.lastname: Pinto
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Rezende
authors.forename: Meriane P
authors.initials: MP
authors.lastname: Carvalho
authors.forename: Rosângela A
authors.initials: RA
authors.lastname: Epifanio
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D002845:Chromatography; D004524:Eels; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D013057:Spectrum Analysis; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007211:Indoles; D010936:Plant Extracts