Protective effects of panduratin A against oxidative damage of tert-butylhydroperoxide in human HepG2 cells.
The protective effect of panduratin A, isolated from Kaempferia pandurata ROXB. (Zingiberaceae), against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity was investigated in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay) was used to monitor cytotoxicity. Lipid peroxidation [malondialdehyde (MDA) formation] and intracellular glutathione level were estimated by fluorometric methods. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA). Panduratin A significantly reduced the cell growth inhibition caused by t-BHP. Furthermore, panduratin A ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation, and attenuated glutathione (GSH) depletion in a dose-dependent manner. It was also found that panduratin A reduced intracellular ROS formation caused by t-BHP. These results strongly suggest that panduratin A has significant protective ability against oxidative damage caused by reactive intermediates.
Sohn
Jong Hee
JH
Department of Biotechnology, Yonsei University, Seoul, Korea.
Han
Kyu-Lee
KL
Lee
Sun-Hee
SH
Hwang
Jae-Kwan
JK
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D047188:Chalcones; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome; D030521:Zingiberaceae; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*
D000975:Antioxidants; D047188:Chalcones; D010936:Plant Extracts; C482884:panduratin A; D020122:tert-Butylhydroperoxide