1-ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization.
1-ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at approximately 420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine.
Wagner
Clemens
C
Technical University of Munich, Chemistry Department, Lichtenbergstr. 4, D-85747 Garching, Germany.
Rist
Manuela
M
Mayer-Enthart
Elke
E
Wagenknecht
Hans-Achim
HA
D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D011721:Pyrenes; C042881:1-ethynylpyrene; D006147:Guanine; D003596:Cytosine; D004247:DNA