title: Stimulation of the XPB ATP-dependent helicase by the beta subunit of TFIIE.
abstract: TFIIE and TFIIH are essential for the promoter opening and escape that occurs as RNA polymerase II transits into early elongation. XPB, a subunit of TFIIH, contains an ATP-dependent helicase activity that is used in both of these processes. Here, we show that the smaller beta subunit of TFIIE stimulates the XPB helicase and ATPase activities. The larger alpha subunit can use its known inhibitory activity to moderate the stimulation by the beta subunit. Regions of TFIIE beta required for the helicase stimulation were identified. Mutants were constructed that are defective in stimulating the XPB helicase but still allow intact TFIIE to bind and recruit XPB and TFIIH to form the pre-initiation complex. In a test for the functional significance of the stimulatory effect of TFIIE beta, these mutant forms of TFIIE were shown to be defective in a transcription assay on linear DNA. The data suggest that the beta subunit of TFIIE is an ATPase and helicase co-factor that can assist the XPB subunit of TFIIH during transcription initiation and the transition to early elongation, enhancing the potential diversity of regulatory targets.
authors.affiliation: Department of Chemistry and Biochemistry, The Molecular Biology Institute, University of California Los Angeles Los Angeles, CA 90095-1569, USA.
authors.forename: Yin C
authors.initials: YC
authors.lastname: Lin
authors.forename: Jay D
authors.initials: JD
authors.lastname: Gralla
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics; D019473:Transcription Factors, TFII / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D021122:Protein Subunits; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE; C072022:XPBC-ERCC-3 protein; D000251:Adenosine Triphosphatases; D004265:DNA Helicases