In vitro characterization of an Fe(8) cluster as potential MRI contrast agent.
The complex [(tacn)(6)Fe(8)(micro(3)-O)(2)(micro(2)-OH)(12)]Br(8).9H(2)O (Fe(8)) was evaluated in vitro as a new kind of possible MRI contrast agent. Relaxivities were measured at 1.41 and 9.4 T for Fe(8) and commercial Gd-DTPA dissolved in PBS. There was significant difference for r(1) and r(2) values between Fe(8) and Gd-DTPA at high field (9.4 T) and for r(1) at low field (1.4 T) (p<0.05). Phantom studies with T(1)-weighted MRI at 9.4 T suggest T(1) contrast potential for Fe(8). That is, up to 5.2 times higher intensity enhancement with respect to that of equimolar Gd-DTPA was obtained with an Fe(8) concentration, referred to the whole molecule, of 0.2 mM, for which no toxicity on C6 cells could be detected. No toxic effects on cultured C6 cells were observed up to a concentration of 1 mM Fe(8).
Rodríguez
Elisenda
E
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, 08193 Bellaterra, Spain. elisenda@icmab.es
Roig
Anna
A
Molins
Elies
E
Arús
Carles
C
Quintero
María Rosa
MR
Cabañas
Miquel E
ME
Cerdán
Sebastián
S
Lopez-Larrubia
Pilar
P
Sanfeliu
Coral
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003287:Contrast Media / Q000009:adverse effects*; D005240:Feasibility Studies; D005910:Glioma / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods*; D058085:Iron Compounds / Q000009:adverse effects*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051381:Rats
D003287:Contrast Media; D058085:Iron Compounds