title: [Infection with chlamydia pneumoniae increases oxidative stress and accelerates the development of atherosclerosis in C57BL/6J mice].
abstract: OBJECTIVE
  To evaluate the effects of Chlamydia pneumoniae infection on oxidative stress and the development of atherosclerosis in C57BL/6J mice.
  
  
  METHODS
  Forty-eight C57BL/6J mice were divided into 4 groups including infection of CP and cholesterol diet, cholesterol diet, infection of CP and control. Atherosclerotic lesions were measured in the aortic root at 40 weeks after the primary infection. Production of superoxide was measured by lucigenin-enhanced chemiluminescence response and evaluated in situ with laser scanning confocal microscope.
  
  
  RESULTS
  Infected mice fed with an atherogenic diet developed significantly larger lesion areas compared with the single atherogenic diet mice (135 249 +/- 43 748 microm2 vs. 96 378 +/- 30 945 microm2, P < 0.05). Superoxide generation was higher in aortic arches of the infected mice or atherogenic diet mice compared with the control mice (1974.25 +/- 650.49, 701.00 +/- 105.16, 455.62 +/- 77.54 counts.mg(-1).min(-1) vs. 142.25 +/- 31.82 counts.mg(-1).min(-1), respectively, P < 0.001).
  
  
  CONCLUSIONS
  Chlamydia pneumoniae infection accelerates atherosclerotic lesion development in diet-induced hypercholesterolemic mice. Generation of reactive oxygen species may contribute to atherosclerotic development by Chlamydia pneumoniae infection.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Zhong Shan University, Guangzhou 510080, China.
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Yu-gang
authors.initials: YG
authors.lastname: Dong
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D050197:Atherosclerosis / Q000209:etiology*; D002690:Chlamydia Infections / Q000150:complications* / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D013481:Superoxides / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D013481:Superoxides