Flow injection spectrofluorimetric study of the supramolecular interaction between beta-cyclodextrin and dequalinium chloride and its analytical application.
The supramolecular interaction of dequalinium chloride (DQC) and beta-cyclodextrin has been studied by flow injection spectrofluorimetry. The results showed that beta-CD reacted with DQC to form a 1:1 host:guest complex with an apparent association constant of 4.99 x 10(2) L mol(-1). Based on the enhancement of the fluorescence intensity of DQC, a flow injection spectrofluorometric method for the determination of DQC in bulk aqueous solution in the presence of beta-CD was developed. The linear range was 0.0412-30.00 microg mL(-1). The detection limit was 12.3 ng mL(-1) with a sampling rate of 80 h(-1). There was no interference from the excipients normally used in tablets and serum compositions. The proposed method was successfully applied to the determination of DQC in tablets and serum.
Tang
Bo
B
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China. tangb@sdnu.edu.cn
Jia
Baoxiu
B
Shi
Xifeng
X
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003868:Dequalinium / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013607:Tablets / Q000737:chemistry; D013816:Thermodynamics; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D013607:Tablets; D047392:beta-Cyclodextrins; D003868:Dequalinium