Neospora caninum: adoptive transfer of immune lymphocytes precipitates disease in BALB/c mice.
Neospora caninum is a recently described apicomplexan parasite first isolated from a dog in 1988 and has subsequently been shown to infect a wide range of mammals. In mice, Neospora can cause primary pneumonia, myositis, encephalitis, radiculoneuritis, and pancreatitis. Whereas, certain aspects of the host immune response to Toxoplasma gondii have been well studied, not as much is known about the full immune response to Neospora. This paper examines whether or not immune splenocytes are able to adoptively transfer protection against N. caninum infection in BALB/c mice. Mice receiving immune enriched CD8+ cells had severe neurological signs by 19 days post infection. Mice receiving immune enriched CD4+ cells had mild neurological signs on day 22 post infection. It would appear that additional immune cells can precipitate disease in the presence of circulating lymphocytes.
Spencer
Jennifer A
JA
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849, USA. spencja@vetmed.auburn.edu
Higginbotham
Michael J
MJ
Young-White
Robert R
RR
Guarino
Anthony J
AJ
Blagburn
Byron L
BL
D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D003048:Coccidiosis / Q000209:etiology / Q000276:immunology* / Q000469:parasitology; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D013997:Time Factors
D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma