title: Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled receptor.
abstract: The seven-transmembrane-spanning G protein-coupled receptor (GPCR) superfamily plays many important roles in basic biology, human health, and human disease. Here, well-resolved solution NMR spectra are presented for a human GPCR, the vasopressin V2 receptor in detergent micelles. The quality of the NMR spectra indicates that backbone resonance assignments for a majority of resonances are feasible. The key to obtaining high quality spectra appears to be the coupling of methods for expressing the receptor into membranes rather than into inclusion bodies, with use of a biochemically mild lysolipid detergent for membrane extraction, protein purification, and NMR sample preparation.
authors.affiliation: Center for Structural Biology and Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-8725, USA.
authors.forename: Changlin
authors.initials: C
authors.lastname: Tian
authors.forename: Richard M
authors.initials: RM
authors.lastname: Breyer
authors.forename: Hak Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Murthy D
authors.initials: MD
authors.lastname: Karra
authors.forename: David B
authors.initials: DB
authors.lastname: Friedman
authors.forename: Anne
authors.initials: A
authors.lastname: Karpay
authors.forename: Charles R
authors.initials: CR
authors.lastname: Sanders
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D017483:Receptors, Vasopressin / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012996:Solutions
chemical_list: D043562:Receptors, G-Protein-Coupled; D017483:Receptors, Vasopressin; D011994:Recombinant Proteins; D012996:Solutions