title: High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?
abstract: Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.
authors.affiliation: Institut de Biochimie et de Biophysique Moléculaire et Cellulaire (CNRS-UMR 8619), Université Paris-Sud, Bâtiment 430, 91405 Orsay, France. nicolas.leulliot@ibbmc.u-psud.fr
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Lionel
authors.initials: L
authors.lastname: Trésaugues
authors.forename: Michael
authors.initials: M
authors.lastname: Bremang
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sorel
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ulryck
authors.forename: Marc
authors.initials: M
authors.lastname: Graille
authors.forename: Ilham
authors.initials: I
authors.lastname: Aboulfath
authors.forename: Anne
authors.initials: A
authors.lastname: Poupon
authors.forename: Dominique
authors.initials: D
authors.lastname: Liger
authors.forename: Sophie
authors.initials: S
authors.lastname: Quevillon-Cheruel
authors.forename: Joël
authors.initials: J
authors.lastname: Janin
authors.forename: Herman
authors.initials: H
authors.lastname: van Tilbeurgh
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D012371:Robotics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins