[Effect of heavy metal cations on the activity of membrane Na+, K+ -ATPase in embryos of loach (Misgurnus fossilis L.)].
The activity of membrane Na+, K+ -ATPase of embryos of loach (Misgurnus fossilis L.) at early stages of development in the normal conditions and under the influence of heavy metal cations has been investigated. It was established, that the influence of such heavy metal cations as Ni2+, Co2+, Sn2+, Zn2+, Mn2+ and Cd2+ in concentrations 10(-6) - 10(-4) M results in reduction of activity of membrane Na+, K+ -ATPase of loach embryos. It was shown, that the inhibition effect is more expressed with the increase of concentrations of heavy metal cations in the incubation medium. The definition of inhibition constants Io.5 has allowed to analyze the sensitivity of Na+, K+ -ATPase to influence of various cations of heavy metals at different stages of blastomer division. Possible mechanisms of influence of heavy metal cations on the activity of membrane Na+, K+ -ATPase of loach embryos have been considered.
Boĭko
N M
NM
Tselevych
M V
MV
Sanahurs'kyĭ
D I
DI
D000818:Animals; D001757:Blastomeres / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D002413:Cations, Divalent / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D003531:Cypriniformes / Q000196:embryology*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019216:Metals, Heavy / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D002413:Cations, Divalent; D004791:Enzyme Inhibitors; D019216:Metals, Heavy; D000254:Sodium-Potassium-Exchanging ATPase