Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover.
Both cellulase and cellobiase can be effectively recovered from hydrolyzed biomass using an ultrafiltration recovery method. Recovery of cellulase ranged from 60 to 66.6% and for cellobiase from 76.4 to 88%. Economic analysis shows that cost savings gained by enzyme recycling are sensitive to enzyme pricing and loading. At the demonstrated recovery of 60% and current loading of 15 Filter paper units of cellulase/g of glucan, enzyme recycling is expected to generate a cost savings of approx 15%. If recovery efficiency can be improved to 70%, the savings will increase to >25%, and at 90% recovery the savings will be 50%.
Steele
Bernie
B
MBI International, 3900 Collins Road, Lansing, MI 48910, USA. steele@mbi.org
Raj
Srini
S
Nghiem
John
J
Stowers
Mark
M
D000641:Ammonia / Q000737:chemistry / Q000191:economics*; D002480:Cellulase / Q000737:chemistry / Q000191:economics* / Q000302:isolation & purification*; D002617:Chemical Industry / Q000191:economics / Q000379:methods; D003198:Computer Simulation; D003362:Cost-Benefit Analysis; D018803:Models, Economic; D010936:Plant Extracts / Q000737:chemistry / Q000191:economics; D014462:Ultrafiltration / Q000191:economics* / Q000379:methods; D014481:United States; D003313:Zea mays / Q000737:chemistry / Q000191:economics*; D001617:beta-Glucosidase / Q000737:chemistry / Q000191:economics* / Q000302:isolation & purification*
D010936:Plant Extracts; D000641:Ammonia; D001617:beta-Glucosidase; D002480:Cellulase