CpG motif in ATCGAT hexamer improves DNA-vaccine efficiency against lethal Pseudorabies virus infection in pigs.
This study evaluated the adjuvant activity of a porcine-specific type A CpG-containing oligodeoxynucleotide (CpG-ODN) in a DNA-vaccine against Pseudorabies virus (PrV) infection. This vaccine, composed of three independent plasmids encoding PrV gB, gC and gD glycoproteins, was injected alone or was co-injected with CpG-ODN or control GpC-ODN. CpG-ODN improved the PrV-specific humoral immune response and provided better clinical protection against lethal PrV-infection. The PrV-specific cell-mediated immune response seemed also to be increased.
Dory
Daniel
D
Viral Genetics and Biosafety Unit, Afssa, BP-53, Fr-22440 Ploufragan, France. d.dory@ploufragan.afssa.fr
Béven
Véronique
V
Torché
Anne-Marie
AM
Bougeard
Stéphanie
S
Cariolet
Roland
R
Jestin
André
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001835:Body Weight; D007114:Immunization; D007371:Interferon-gamma / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011557:Pseudorabies / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D022421:Pseudorabies Vaccines; D012333:RNA, Messenger; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma