Comparison of ELISA and LC-MS analyses for yessotoxins in blue mussels (Mytilus edulis).
Blue mussels (Mytilus edulis) collected from Flødevigen Bay, Norway, in 2001 and 2002 were analysed for yessotoxins (YTXs) by ELISA and yessotoxin (YTX), 45-hydroxyYTX, and carboxyYTX by LC-MS. Results from the two methods were compared to evaluate the ELISA. The response in the ELISA was 3-13 times higher than LC-MS, probably due to the antibodies binding to other YTX analogues not included in the LC-MS analysis. Nevertheless, the correlation between ELISA and LC-MS was good, with r2 values> or =0.8. The results indicate that the ELISA is a reliable method for estimating the total level of YTXs in mussels, and are consistent with extensive metabolism of algal YTXs in mussels. YTX was a minor component in the blue mussels at all times compared to 45-hydroxyYTX and especially carboxyYTX, except when the P. reticulatum bloom occurred. The results also indicate the presence of significant amounts of YTX analogues in addition to those measured by LC-MS. All samples below 4 mg/kg by ELISA were below the current EU regulatory limit of 1 mg/kg by LC-MS. Therefore, we propose using ELISA as a screening tool with a cut-off limit at 4 mg/kg for negative samples, whereas samples above this limit would be reanalyzed by LC-MS.
Samdal
Ingunn A
IA
National Veterinary Institute, P.O. Box 8156 Dep., N-0033 Oslo, Norway. ingunn.samdal@vetinst.no
Aasen
John A B
JA
Briggs
Lyn R
LR
Dahl
Einar
E
Miles
Christopher O
CO
D000818:Animals; D049872:Bivalvia / Q000737:chemistry* / Q000502:physiology; D002853:Chromatography, Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004988:Ethers, Cyclic / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D008978:Mollusk Venoms / Q000032:analysis*; D009664:Norway; D035964:Oxocins / Q000032:analysis*; D012621:Seasons
C419348:45-hydroxyyessotoxin; D004988:Ethers, Cyclic; D008978:Mollusk Venoms; D035964:Oxocins; C503829:carboxyyessotoxin; C066632:yessotoxin