title: Kinetics of enzyme-catalyzed alcoholysis of soybean oil in n-hexane.
abstract: This work investigated the production of fatty acid ethyl esters (FAEEs) from soybean oil using n-hexane as solvent and two commercial lipases as catalysts, Novozym 435 and Lipozyme IM. A Taguchi experimental design was adopted considering the variables temperature (35-65 degrees C), addition of water (0-10 wt/wt%), enzyme (5-20 wt/wt%) concentration, and oil-to-ethanol molar ratio (1:3-1:10). It is shown that complete conversion in FAEE is achieved for some experimental conditions. The effects of process variables on reaction conversion and kinetics of the enzymatic reactions are presented for all experimental conditions investigated in the factorial design.
authors.affiliation: Department of Food Engineering, URI, Campus de Erechim, Av. Sete de Setembro, 1621-Erechim-RS, 99700-000, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: Irajá
authors.initials: I
authors.lastname: do Nascimento Filho
authors.forename: Marco
authors.initials: M
authors.lastname: Di Luccio
authors.forename: Carina
authors.initials: C
authors.lastname: Faccio
authors.forename: Clarissa
authors.initials: C
authors.lastname: Dalla Rosa
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Bender
authors.forename: Nádia
authors.initials: N
authors.lastname: Lipke
authors.forename: Cristiana
authors.initials: C
authors.lastname: Amroginski
authors.forename: Cláudio
authors.initials: C
authors.lastname: Dariva
authors.forename: José Vladimir
authors.initials: JV
authors.lastname: de Oliveira
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004952:Esters; D005227:Fatty Acids / Q000138:chemical synthesis*; D005656:Fungal Proteins; D006586:Hexanes / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D012996:Solutions; D012997:Solvents / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry*
chemical_list: D000438:Alcohols; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D005656:Fungal Proteins; D006586:Hexanes; D012996:Solutions; D012997:Solvents; C026385:n-hexane; D013024:Soybean Oil; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase