Application of HPLC for determination of phytic acid in the colonic epithelial Caco-2 cells.
Phytic acid (myo-inositol hexaphosphate, IP6) is currently receiving a considerable interest because of its anticancer (preventive and therapeutic) potential against colon tumors and the need for methods of its determination. The aim of this study was to analyze the uptake of IP6 by human colon adenocarcinoma cells (Caco-2 cell line) and to evaluate the method of its intracellular quantification with the use of high performance liquid chromatography (HPLC) technique. Chromatographic analysis revealed a rapid uptake of IP6 by cells. The intracellular accumulation of IP6 was saturable at 0.5 h and it did not change with the prolongation of incubation up to 72 h.
Weglarz
Ludmiła
L
Department of Biochemistry, Medical University of Silesia 1 Narcyzów Str., 41-200 Sosnowiec, Poland.
Parfiniewicz
Beata
B
Bat
Beata
B
Orchel
Arkadiusz
A
Dzierzewicz
Zofia
Z
Wilczok
Tadeusz
T
D018938:Caco-2 Cells; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010833:Phytic Acid / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D010833:Phytic Acid