Proteomic analysis of ursolic acid-induced apoptosis in cervical carcinoma cells.
Proteomic analyses can efficiently detect the variation of protein in high throughput screening. Using 2DE/MALDI-TOF-MS and SELDI-TOF-MS, we tried to search several cellular proteins that are responsive to ursolic acid (UA) in HeLa cervical carcinoma cells. Compared to control, UA-treated HeLa cells unfolded 25 proteins in significant changes by 2DE/MALDI-TOF-MS, most of which were involved in apoptosis. SELDI-TOF-MS with two types of protein chips profiled and analyzed proteomic features after administration of UA. Interestingly, eight polypeptide peaks can be detected. Further identification and characterization of these proteins may build the molecular basis of UA-induced apoptosis and provide insight into the anti-proliferative mechanism in cervical carcinoma cells.
Yim
Eun-Kyoung
EK
Department of Medical Bioscience, Graduate School of Catholic University, Kangnam St Mary's Hospital, The Catholic University, 505 Banpo-dong, Seocho-gu, Seoul, 137-040 South Korea.
Lee
Keun-Ho
KH
Namkoong
Sung-Eun
SE
Um
Soo-Jong
SJ
Park
Jong-Sup
JS
D017209:Apoptosis / Q000187:drug effects*; D002154:Calpain / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D053181:Caspase 8; D020169:Caspases / Q000032:analysis; D056970:Co-Repressor Proteins; D051360:Cyclin-Dependent Kinase 5 / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006367:HeLa Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics; D016601:RNA-Binding Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
D002154:Calpain; D002352:Carrier Proteins; D053181:Caspase 8; D020169:Caspases; D056970:Co-Repressor Proteins; D051360:Cyclin-Dependent Kinase 5; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; D014315:Triterpenes; D000097245:Ursolic Acid; C498650:SAP18 protein, human; C120901:CAPN11 protein, human; C505530:CASP8 protein, human