[Effects of BMP-2 on the gene expression of rat osteosarcoma cells under simulated weightlessness].
OBJECTIVE
To investigate the effect of BMP-2 on (the) gene expression of rat osteosarcoma cells (ROS17/2.8) under rotating clinostat simulated weightlessness.


METHODS
ROS17/2.8 cells were cultivated in 1 G control and rotating clinostat simulated weightlessness with 500 ng/ml BMP-2 in the culture medium. Total RNA in cells was isolated after 24, 48 and 72 h. Reverse transcription PCR analysis was made to examine the gene expression of alpha 1 chain of type I collagen (collagen I alpha 1) and alkaline phosphatase (ALP).


RESULTS
The expression of COL-I alpha 1 mRNA induced by BMP-2 was much more than that without BMP-2 in 24 h and 48 h group (P<0.01). The level of ALP mRNA induced by BMP-2 was significantly high in 48 h or 72 h group (respectively P<0.01, P<0.05). The level of BMP-2 induced expression of COL-I alpha 1 mRNA was significantly lower under 48 h of simulated weightlessness than in 1 G condition (P<0.01). The level of ALP mRNA was significantly lower under simulated weightlessness for 48 h or 72 h (P<0.01).


CONCLUSIONS
BMP-2 can stimulate the differentiation of ROS17/2.8 cells in 1 G condition and this process is reduced under simulated weightlessness.
Wang
Bing
B
Faculty of Aerospace Medicine, The Fourth Military Medical University, Xi'an, China. wbean99@sina.com
Zhang
Shu
S
Wu
Xing-yu
XY
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects*; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D012516:Osteosarcoma / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012399:Rotation; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D018474:Weightlessness Simulation
C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000469:Alkaline Phosphatase