Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis.
We have used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to study the expression of contractile and regulatory proteins in human vastus lateralis and deltoideus muscles, in order to understand protein turnover and isoform switching in muscles with the same fiber-type composition but different functional properties. We demonstrate a two- to six-fold overexpression of enzymes associated with glycolysis, the tricarboxylic acid cycle, oxidative phosphorylation, and substrate transport in vastus lateralis compared to deltoideus. Expression levels of contractile protein isoforms correlated to the proportion of slow-twitch fibers in deltoideus compared to vastus lateralis are consistent with the different contractile properties of the two muscles. Two proteins involved in free radical homeostasis were differentially expressed, suggesting a direct relationship between radical scavenging and the muscle function. The application of 2-DE and MS to studies of muscle physiology thus offers a more comprehensive assessment of the molecular determinants of muscle function than traditional approaches.
Capitanio
Daniele
D
Institute of Molecular Bioimaging and Physiology, CNR, Segrate (MI), Italy.
Viganò
Agnese
A
Ricci
Enzo
E
Cerretelli
Paolo
P
Wait
Robin
R
Gelfi
Cecilia
C
D000328:Adult; D000595:Amino Acid Sequence; D001706:Biopsy; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D004798:Enzymes; D009124:Muscle Proteins; D010446:Peptide Fragments