Preparation of porcine small intestinal submucosa sponge and their application as a wound dressing in full-thickness skin defect of rat.
Small intestinal submucosa (SIS) sponge was prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The prepared SIS sponges exhibited elastic and soft property on touch and were ease to handle. The SIS sponges have the pore diameter of 100-200 microm and an interconnective porous structure. The SIS sponges exhibited high water absorption ability over 8000%. The water uptake of SIS sponges decreased as SIS concentration used to manufacture SIS sponge increased. In wound healing test, SIS sponge attained uniform adherence to the wound surface. The SIS sponges absorbed higher extent of exudation for wound than that covered with Tegaderm as control. Wound area contracted above 80% at the 21st postoperative day. The SIS sponge treated wound was almost completely covered with a thin layer of epidermis at 4 weeks. In addition, the dermal collagen in the wound regenerated at only SIS sponges treated wounds. The progress of granulous tissue formation was faster in SIS sponges as wound dressing than in Tegaderm. In conclusion, we found that the SIS sponges might be a potential material as a wound dressing.
Kim
Moon Suk
MS
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Republic of Korea.
Hong
Keum Duck
KD
Shin
Hye Won
HW
Kim
Seon Hwa
SH
Kim
Soon Hee
SH
Lee
Min Suk
MS
Jang
Woo Young
WY
Khang
Gilson
G
Lee
Hai Bang
HB
D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D001684:Biological Dressings; D001706:Biopsy; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D007249:Inflammation; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011161:Porifera; D011208:Powders / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones / Q000737:chemistry; D012867:Skin / Q000378:metabolism; D017768:Skin, Artificial; D013552:Swine; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014945:Wound Healing
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011208:Powders; D012828:Silicones; D014867:Water; D005022:Ethyldimethylaminopropyl Carbodiimide