Identification of antiadhesive fraction(s) in nonimmunized egg yolk powder: in vitro study.
The presence of antiadhesive component(s) in the hen egg yolk against foodborne pathogens was anticipated from results of a previous animal study conducted by the authors. The previous work showed egg yolk powder without specific antibodies is effective in controlling Salmonella enteritidis,Salmonella typhimurium, and Escherichia coli O157:H7 colonization in laying hens. Therefore, this study was necessary to locate the activity and identify the effective component(s). In vitro experiments were conducted using confluent Caco-2 cell monolayers. S. enteritidis, S. typhimurium, and E. coli O157:H7 were investigated against the various extracted granule and plasma fractions in three different assays: adhesion elimination, adhesion prevention, and antimicrobial. This study revealed original findings and identified the protective yolk fraction against the foodborne pathogens as the granule component, high-density lipoproteins (HDL). The protective activity conveyed by HDL was confirmed to remain intact despite peptic and tryptic enzymatic digestion and to have antiadhesive but not antimicrobial effect.
Kassaify
Zeina G
ZG
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Li
Esther W Y
EW
Mine
Yoshinori
Y
D000818:Animals; D000890:Anti-Infective Agents; D001422:Bacterial Adhesion / Q000187:drug effects*; D002645:Chickens / Q000382:microbiology; D004530:Egg Yolk / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D019453:Escherichia coli O157 / Q000187:drug effects; D005260:Female; D005516:Food Microbiology; D008075:Lipoproteins, HDL / Q000032:analysis / Q000494:pharmacology; D010455:Peptides / Q000032:analysis / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects
D000890:Anti-Infective Agents; D008075:Lipoproteins, HDL; D010455:Peptides