title: The ischemic rat heart releases S100B.
abstract: S100B is an astrocytic protein assessed in cerebrospinal fluid and serum as a biochemical marker of cerebral injuries. However, increasing evidences suggest the influence of extra cerebral sources on its serum levels. Since it was reported that the injured myocardium expresses S100B, we investigated whether the isolated heart releases this protein. The rat hearts were excised and perfused by the Langendorff technique of isolated heart perfusion. After stabilization, 10 hearts (ischemic group) were submitted to 20 minutes of ischemia and 30 minutes of reperfusion, and 5 hearts (control group) were submitted to 50 minutes of perfusion. The perfusion fluid was collected at pre-ischemia, and 0, 5, 10, 15 and 30 min after ischemia (or equivalent in controls) for S100B and cardiac troponin T (a heart injury marker) assays. In the ischemic group, S100B and troponin T levels increased significantly at time 0 min: S100B values [mug/L, median (IQ25/IQ75)] increased from < or = 0.02 (< or = 0.02/0.03) to 0.38 (0.22/0.84), while troponin T values [mug/L, median (IQ25/IQ75)] increased from 0.31 (0.15/0.45) to 2.84 (2.00/3.63). Our results point to the ischemic heart as an extra cerebral source of S100B.
authors.affiliation: Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2600, anexo.CEP 90035-003. Porto Alegre, RS, Brazil.
authors.forename: Guilherme S
authors.initials: GS
authors.lastname: Mazzini
authors.forename: Débora V
authors.initials: DV
authors.lastname: Schaf
authors.forename: Alvaro R
authors.initials: AR
authors.lastname: Oliveira
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gonçalves
authors.forename: Adriane
authors.initials: A
authors.lastname: Belló-Klein
authors.forename: Solange
authors.initials: S
authors.lastname: Bordignon
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Bruch
authors.forename: Guilherme F
authors.initials: GF
authors.lastname: Campos
authors.forename: Dalton V
authors.initials: DV
authors.lastname: Vassallo
authors.forename: Diogo O
authors.initials: DO
authors.lastname: Souza
authors.forename: Luis V C
authors.initials: LV
authors.lastname: Portela
mesh_terms: D000818:Animals; D005260:Female; D006321:Heart / Q000502:physiology*; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D020107:Troponin T / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; D020107:Troponin T