title: [Modification of a method for determining the cytotoxic activity of lymphocytes--natural killers in space experiments].
abstract: A space modification of a method for determining the cytotoxic activity of natural killers (NK) uses cultures of human lymphocytes and re-inoculated suspension of tumorous myeloblasts K-562. The 3H-uridine labeled target-cells fixated in 0.1 % formalin solution proved to be best suited to the conditions prohibiting regular recharge of cultural medium and utilization of radioactive agents. The following practical conclusions were made from the space-flown experiment: pre-launch procedures (lymphocyte isolation and delivery to the launch site) and time period till dock must not be longer than 5 days (1) and during transportation to the orbiting vehicle cell cultures must be kept at 18-20 degrees C (2). The modified method can be employed to study various aspects of cell interaction such as the NK ability to destroy target-cells in suspension cultured exposed in microgravity.
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
authors.forename: M P
authors.initials: MP
authors.lastname: Rykova
authors.forename: E N
authors.initials: EN
authors.lastname: Antropova
authors.forename: O V
authors.initials: OV
authors.lastname: Grigor'eva
mesh_terms: D023181:Antimicrobial Cationic Peptides; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology* / Q000502:physiology; D042381:Granulocyte Precursor Cells / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology; D007951:Leukemia, Myeloid / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology; D013026:Space Flight; D013696:Temperature; D013997:Time Factors; D014529:Uridine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C026530:myroridin K; D014529:Uridine