title: Metabolic effects of ethanol on primary cell cultures of rat skeletal muscle.
abstract: Individuals who have consumed alcohol chronically accumulate glycogen in their skeletal muscles. Changes in the energy balance caused by alcohol consumption might lead to alcoholic myopathy. Experimental models used in the past, such as with skeletal muscle biopsy samples of alcohol-dependent individuals or in animal models, do not distinguish between direct effects and indirect effects (i.e., alterations to the nervous or endocrine system) of alcohol. In the current study, we evaluated the direct effect of ethanol on skeletal muscle glycogen concentrations and related glycolytic pathways. We measured the changes in metabolite concentrations and enzyme activities of carbohydrate metabolism in primary cell cultures of rat skeletal muscle exposed to ethanol for two periods. The concentrations of glycolytic metabolites and the activities of several enzymes that regulate glucose and glycogen metabolism were measured. After a short exposure to ethanol (6 h), glucose metabolism slowed. After 48 h of exposure, glycogen accumulation was observed.
authors.affiliation: Muscle Research Group, Department of Physiological Science I, Universitat de Barcelona, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Garriga
authors.forename: Joaquim
authors.initials: J
authors.lastname: Fernández-Solá
authors.forename: Ester
authors.initials: E
authors.lastname: Adanero
authors.forename: Alvaro
authors.initials: A
authors.lastname: Urbano-Márquez
authors.forename: Roser
authors.initials: R
authors.lastname: Cussó
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4; D006003:Glycogen / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D025481:Phosphofructokinase-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat; D000431:Ethanol; D006003:Glycogen; D025481:Phosphofructokinase-2; D005947:Glucose