Granulocyte colony-stimulating factor is not protective against selective dopaminergic cell death in vitro.
In the present study, we evaluated the potential neuroprotective effect of granulocyte colony-stimulating factor (G-CSF), a hematopoietic growth factor in two different culture models in which dopaminergic (DA) neurons die selectively: first, in a culture model in which death of DA neurons occurs spontaneously and second, in a toxin-based paradigm, the in vitro 1-methyl-4-phenylpyridinium model of PD. In neither of the two models, a treatment with G-CSF, could prevent or halt the progressive neurodegeneration. However, we cannot rule out that G-CSF might exert neuroprotective or even deleterious effects in in vivo models of PD, based on the significant increase in the number of microglial cells observed after G-CSF treatment.
Henze
Carmen
C
INSERM U 679 (former U 289), Experimental Neurology and Therapeutics, Centre d'Investigation Clinique, Hôpital de la Salpêtrière, Paris, France.
Lescot
Thomas
T
Traver
Sabine
S
Salthun-Lassalle
Bénédicte
B
Hirsch
Etienne C
EC
Michel
Patrick P
PP
Hartmann
Andreas
A
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003561:Cytarabine / Q000633:toxicity; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006540:Herbicides / Q000633:toxicity; D007150:Immunohistochemistry / Q000379:methods; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000493:pharmacokinetics; D014316:Tritium / Q000493:pharmacokinetics; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D006540:Herbicides; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D014746:Vimentin; D003561:Cytarabine; D014316:Tritium; D016179:Granulocyte Colony-Stimulating Factor; D014446:Tyrosine 3-Monooxygenase; D015655:1-Methyl-4-phenylpyridinium; D013936:Thymidine; D004298:Dopamine