[The STR typing system by fluorescence labeled Multiplex-PCR technique and its forensic application].
OBJECTIVE
To build the four STR loci typing system by fluorescence labeled Multiplex-PCR technique, applied in the parentage test and personal identification in forensic medicine.


METHODS
The primer of D3S1754 and D1S549 were labeled with 6-FAM and TMR respectively, primers of D4S2366 and D12S375 were labeled with HEX. Multiplex-PCR products were analysed on the ABI PRISM 310 Genetic Analyzer where the Data Collection Software 3.0, the GeneScan Analysis Software 3.7NT and the Genotyper 3.7NT Software were used. This typing system has been emploied in the parentage test and personal identification of casework.


RESULTS
A method of typing four STR loci by fluorescence labeled Multiplex-PCR technique had been constructed. It has showed good sensitive and stability, and met the needs of parentage test and personal identification in forensic medicine.


CONCLUSIONS
The constructed method can be used in studying genetic polymorphisms and parentage test or personal identification in forensic medicine.
Li
Ying-bi
YB
School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China. liyingbiscu@163.com
Wu
Jing
J
Hou
Yi-ping
YP
D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D016172:DNA Fingerprinting; D017931:DNA Primers; D005269:Femur; D005554:Forensic Medicine; D005787:Gene Frequency; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012984:Software; D013050:Spectrometry, Fluorescence; D020080:Tandem Repeat Sequences / Q000235:genetics*
D017931:DNA Primers; D004247:DNA