title: [Expression of bFGF receptor and TGF-beta receptors in cultured human scleral fibroblasts].
abstract: OBJECTIVE
  To examine the expression of basic fibroblast growth factor receptor (FGF R1) and transforming growth factor-beta receptors (TGF-beta RI and TGF-beta RII) in cultured human scleral fibroblasts.
  
  
  METHODS
  Scleral fibroblasts of passages 2-4 were used for the present studies. Polyclonal antibodies against FGF R1, TGF-beta RI and TGF-beta RII were used to detect the proteins of these receptors. Indirect immunofluorescence staining method (IIF) was used.
  
  
  RESULTS
  Antibodies for FGF R1, TGF-beta RI and TGF-beta RII produced specific staining of the entire cell surface, including the cell membrane enveloping cytoplasmic projections; positive staining in some cells was most intensive in the perinuclear region. Immunostaining mainly originated from the cell membranes, indicating that the presence of the receptor proteins on the cell surface. The intensity of staining for TGF-beta RI and TGF-beta RII was relatively strong, while staining of FGF R1 was relatively weak. The cells treated with PBS instead of primary antibodies did not produce specific staining.
  
  
  CONCLUSIONS
  This study shows that cultured human scleral fibroblasts express the receptor protein for FGF R1, TGF-beta RI and TGF-beta RII and also indicates that these growth factors may influence these cells. Exogenous bFGF and TGF-beta administration may elicit actions through the binding with these receptors in the scleral fibroblasts.
authors.affiliation: School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou 325003, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Qu
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Xiang-tian
authors.initials: XT
authors.lastname: Zhou
authors.forename: Dan-ning
authors.initials: DN
authors.lastname: Hu
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Fu
authors.forename: Fan
authors.initials: F
authors.lastname: Lü
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D009216:Myopia; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D012590:Sclera / Q000166:cytology*
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2