Lecithin:retinol acyltransferase in ARPE-19.
The purpose of this study is to investigate if a readily available cell line (APRE-19) may be used to study in vitro function of visual cycle enzymes such as lecithin:retinol acyltransferase (LRAT). Cells incubated with exogenous retinol accumulated intracellular all-trans retinol and all-trans retinyl ester. Membrane proteins from ARPE-19 exhibited LRAT activity, which was inhibited by an LRAT inhibitor, retinyl bromoacetate (RBA). Gene microarray and Western blot results indicated that ARPE-19 cells expressed LRAT transcript and the LRAT protein. Therefore, our data show that ARPE-19 contains an active LRAT enzyme and suggest that it is an appropriate cell system to study visual cycle enzymes.
Trevino
Simon G
SG
Department of Biology, the University of Texas at San Antonio, San Antonio, TX 78249, USA.
Schuschereba
Steven T
ST
Bowman
Phillip D
PD
Tsin
Andrew
A
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D005136:Eye Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D012176:Retinoids / Q000378:metabolism
D005136:Eye Proteins; D008565:Membrane Proteins; D012176:Retinoids; D000217:Acyltransferases; C056942:lecithin-retinol acyltransferase