title: Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus.
abstract: Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
authors.affiliation: Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, 27599-7431, USA. JIN2@cdc.gov
authors.forename: N
authors.initials: N
authors.lastname: Jothikumar
authors.forename: T L
authors.initials: TL
authors.lastname: Cromeans
authors.forename: M D
authors.initials: MD
authors.lastname: Sobsey
authors.forename: B H
authors.initials: BH
authors.lastname: Robertson
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D017931:DNA Primers; D004196:Disease Outbreaks; D004784:Environmental Monitoring / Q000379:methods; D062665:Epidemiological Monitoring; D006506:Hepatitis A / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D030041:Hepatitis A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D012367:RNA, Viral; D019914:Taq Polymerase