Simultaneous quantification of six ephedrines in a Mahwang preparation and in urine by high-performance liquid chromatography.
A rapid and reliable high-performance liquid chromatographic method was developed and validated for the simultaneous determination of norephedrine (NE), norpseudoephedrine (NPE), ephedrine (E), pseudoephedrine (PE), methylephedrine (ME) and methylpseudoephedrine (MPE) in both a Mahwang traditional Chinese medicine (TCM) preparation and in urine using alpha-ethylbenzylamine as the internal standard. The method uses a Spherisorb C(18) column for an isocratic elution in a tetraethylammoniumphosphate-methanol mobile phase at a wavelength of 206 nm. The limits of detection of NE, NPE, E, PE, ME and MPE in sample solutions ranged from 0.1 to 0.3 microg[sol ]mL at a signal-to-noise ratio of 3. The within-day precision as calculated from the Mahwang TCM preparation and urine samples was below 6.2 and 1.4% for each analyte. The between-day precision as calculated from the Mahwang TCM preparation and urine samples was below 6.8 and 5.9% for each analyte. The between-day accuracy as determined from the Mahwang TCM preparation and urine samples was below 2.2 and 6.8% for each analyte. The recoveries for six compounds, obtained with compounds spiked into the Mahwang TCM preparation and urine, were found to be more than 93.6%. This method can be successfully applied to doping and excretion rate studies.
Chan
Kuei Hui
KH
Sports Business Management Department, Da Yah University, Da-Tsuen, Changhua, Taiwan, Republic of China.
Pan
Ryh-Nan
RN
Hsu
Mei-Chich
MC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004300:Doping in Sports; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004809:Ephedrine / Q000031:analogs & derivatives / Q000032:analysis / Q000652:urine; D006801:Humans; D010665:Phenylpropanolamine / Q000032:analysis / Q000652:urine; D011412:Propanolamines / Q000032:analysis* / Q000652:urine*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D011412:Propanolamines; C005331:norpseudoephedrine; D010665:Phenylpropanolamine; C041707:N-methylephedrine; D004809:Ephedrine