Synthetic nanoscale elements for delivery of materials into viable cells.
Arrays of vertically aligned carbon nanofibers (VACNFs) provide structures that are well suited for the direct integration and manipulation of molecular-scale phenomena within intact, live cells. VACNFs are fabricated via a combination of microfabrication techniques and catalytic plasma-enhanced chemical vapor deposition. In this chapter, we discuss the synthesis of VACNFs and detail the methods for introducing these arrays into the intracellular domain of mammalian cells for the purpose of delivering large macromolecules, specifically plasmid DNA, on a massively parallel basis.
McKnight
Timothy E
TE
Molecular-Scale Engineering and Nanoscale Technologies Research Group, Oak Ridge National Laboratory, TN, USA.
Melechko
Anatoli V
AV
Guillorn
Michael A
MA
Merkulov
Vladimir I
VI
Lowndes
Douglas H
DH
Simpson
Michael L
ML
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D018014:Gene Transfer Techniques; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*
D037742:Nanotubes, Carbon