title: Identification of Helicobacter pylori surface proteins by selective proteinase K digestion and antibody phage display.
abstract: Five surface proteins of Helicobacter pylori were identified by proteinase K treatment of live H. pylori followed by proteome analysis. One of the identified proteins, HopQ, is also recognized by an antibody selected by phage display screening of intact H. pylori.
authors.affiliation: Max Planck Institute for Infection Biology, Department of Molecular Biology, Schumannstrasse 21/22, D-10117 Berlin, Germany. nicolas.2.sabarth@gsk.com
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sabarth
authors.forename: Robert
authors.initials: R
authors.lastname: Hurvitz
authors.forename: Monika
authors.initials: M
authors.lastname: Schmidt
authors.forename: Ursula
authors.initials: U
authors.lastname: Zimny-Arndt
authors.forename: Peter R
authors.initials: PR
authors.lastname: Jungblut
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Meyer
authors.forename: Dirk
authors.initials: D
authors.lastname: Bumann
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D019286:Endopeptidase K; D016480:Helicobacter pylori / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D040901:Proteomics
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D019151:Peptide Library; D019286:Endopeptidase K