Effects of hemicellulose and lignin on enzymatic hydrolysis of cellulose from dairy manure.
This study focused on the effect of hemicellulose and lignin on enzymatic hydrolysis of dairy manure and hydrolysis process optimization to improve sugar yield. It was found that hemicellulose and lignin in dairy manure, similar to their role in other lignocellulosic material, were major resistive factors to enzymatic hydrolysis and that the removal of either of them, or for best performance, both of them, improved the enzymatic hydrolysis of manure cellulose. This result combined with scanning electron microscope (SEM) pictures further proved that the accessibility of cellulose to cellulase was the most important feature to the hydrolysis. Quantitatively, fed-batch enzymatic hydrolysis of fiber without lignin and hemicellulose had a high glucose yield of 52% with respect to the glucose concentration of 17 g/L at a total enzyme loading of 1300 FPU/L and reaction time of 160 h, which was better than corresponding batch enzymatic hydrolysis.
Liao
Wei
W
Department of Biological Systems Engineering and Center for Multiphase Environmental Research, and, Washington State University, Pullman, WA 99163, USA. wliao@mail.wsu.edu
Wen
Zhiyou
Z
Hurley
Sharon
S
Liu
Yan
Y
Liu
Chuanbin
C
Chen
Shulin
S
D002241:Carbohydrates / Q000138:chemical synthesis*; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003247:Conservation of Natural Resources / Q000379:methods; D003612:Dairying / Q000379:methods*; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D008031:Lignin / Q000737:chemistry*; D008372:Manure; D011134:Polysaccharides / Q000737:chemistry
D002241:Carbohydrates; D007220:Industrial Waste; D008372:Manure; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase