title: Effects of supplementation with ascorbic acid for a period of 120 days on the myosin-V and NADPHd positive myenteric neurons of the ileum of rats.
abstract: We investigated the effect of ascorbic acid (AA) supplementation on the NADPH-diaphorase (NADPHd) and myosin-V myenteric neurons in the ileum of rats, after 4 months of treatment. Two groups were compared, i.e. controls rats (C) and AA-treated rats (CA). Myosin-V immunohistochemistry and NADPHd histochemistry were employed. We investigated the areas of 500 cell bodies of myosin-V neurons and of 500 NADPHd stained neurons from all groups. The quantitative analysis was performed using an area of 8.96 mm2 from each ileum. There was an increase of 21.9% in the myosin-V immunoreactive myenteric neurons (P > 0.05) and of 22.5% in the NADPHd in group CA when compared with C (P < 0.05). There were no significant differences when we compared the area of myosin-V stained neurons between groups C and CA. However, we verified an area reduction of 7.5% in NADPHd neurons when comparing group C to group CA (P < 0.05).
authors.affiliation: Department of Morphophysiological Sciences, State University of Maringá, PR, Brazil. jnzanoni@uem.br
authors.forename: J N
authors.initials: JN
authors.lastname: Zanoni
authors.forename: P
authors.initials: P
authors.lastname: De Freitas
authors.forename: R V F
authors.initials: RV
authors.lastname: Pereira
authors.forename: M A
authors.initials: MA
authors.lastname: Dos Santos Pereira
authors.forename: M H
authors.initials: MH
authors.lastname: De Miranda Neto
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D007082:Ileum / Q000294:innervation* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects* / Q000378:metabolism; D024701:Myosin Type V; D009245:NADH Dehydrogenase; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009245:NADH Dehydrogenase; D024701:Myosin Type V; D001205:Ascorbic Acid