Expression of functional recombinant mussel adhesive protein type 3A in Escherichia coli.
Mussel adhesive proteins, including the 20-plus variants of foot protein type 3 (fp-3), have been suggested as potential environmentally friendly adhesives for use in aqueous conditions and in medicine. Here we report the novel production of a recombinant Mytilus galloprovincialis foot protein type 3 variant A (Mgfp-3A) fused with a hexahistidine affinity ligand in Escherichia coli and its approximately 99% purification with affinity chromatography. Recombinant Mgfp-3A showed a superior purification yield and better apparent solubility in 5% acetic acid (prerequisites for large-scale production and practical use) compared to those of the previously reported recombinant M. galloprovincialis foot protein type 5 (Mgfp-5). The adsorption abilities and adhesion forces of purified recombinant Mgfp-3A were compared with those of Cell-Tak (a commercial mussel extract adhesive) and recombinant Mgfp-5 using quartz crystal microbalance analysis and modified atomic force microscopy, respectively. These assays showed that the adhesive ability of recombinant Mgfp-3A was comparable to that of Cell-Tak but lower than that of recombinant Mgfp-5. Collectively, these results indicate that recombinant Mgfp-3A may be useful as a commercial bioadhesive or an adhesive ingredient in medical or underwater environments.
Hwang
Dong Soo
DS
Department of Chemical Engineering and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Gim
Youngsoo
Y
Cha
Hyung Joon
HJ
D000268:Adhesiveness; D000269:Adhesives; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008422:Materials Testing; D008970:Molecular Weight; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013314:Stress, Mechanical; D014014:Tissue Adhesives
D000269:Adhesives; D001672:Biocompatible Materials; D011506:Proteins; D011994:Recombinant Proteins; D014014:Tissue Adhesives; C037403:adhesive protein, mussel