AM3 inhibits LPS-induced iNOS expression in mice.
We have analyzed the effect of a patented glycoconjugate of natural origin, AM3 (commercially available under the name Inmunoferon) in the expression of iNOS induced by administration of LPS in mice. We have observed that oral treatment with the drug daily for 6 days reduced the levels of expression of iNOS induced by an intravenous pulse of LPS. This effect was significant in the lungs and kidneys, but it was much more marked in the liver. In addition, the levels of nitric oxide in serum were clearly decreased upon treatment with AM3. Together, these results suggest that AM3 modulates the nitric oxide response and points to a possible role for AM3 in the control of the inflammatory response.
Majano
Pedro
P
Unidad de Biología Molecular, Hospital Universitario de la Princesa, U.A.M., Madrid, Spain.
Alonso-Lebrero
José Luis
JL
Janczyk
Agnieszka
A
Martín-Vichez
Samuel
S
Molina-Jiménez
Francisca
F
Brieva
Aurora
A
Pivel
Juan Pablo
JP
González
Salvador
S
López-Cabrera
Manuel
M
Moreno-Otero
Ricardo
R
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006020:Glycopeptides / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D011917:Rats, Inbred Lew; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000893:Anti-Inflammatory Agents; D002130:Calcium Phosphates; D006020:Glycopeptides; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C038773:Immunoferon; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C496318:Nos2 protein, rat