Automation of macromolecular crystallography beamlines.
The production of three-dimensional crystallographic structural information of macromolecules can now be thought of as a pipeline which is being streamlined at every stage from protein cloning, expression and purification, through crystallisation to data collection and structure solution. Synchrotron X-ray beamlines are a key section of this pipeline as it is at these that the X-ray diffraction data that ultimately leads to the elucidation of macromolecular structures are collected. The burgeoning number of macromolecular crystallography (MX) beamlines available worldwide may be enhanced significantly with the automation of both their operation and of the experiments carried out on them. This paper reviews the current situation and provides a glimpse of how a MX beamline may look in the not too distant future.
Arzt
Steffi
S
European Synchrotron Radiation Facility, 6 rue Jules Horowitz, Zip 38000, BP 220, F-38043 Grenoble Cedex, France.
Beteva
Antonia
A
Cipriani
Florent
F
Delageniere
Solange
S
Felisaz
Franck
F
Förstner
Gabriele
G
Gordon
Elspeth
E
Launer
Ludovic
L
Lavault
Bernard
B
Leonard
Gordon
G
Mairs
Trevor
T
McCarthy
Andrew
A
McCarthy
Joanne
J
McSweeney
Sean
S
Meyer
Jens
J
Mitchell
Edward
E
Monaco
Stephanie
S
Nurizzo
Didier
D
Ravelli
Raimond
R
Rey
Vicente
V
Shepard
William
W
Spruce
Darren
D
Svensson
Olof
O
Theveneau
Pascal
P
D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012984:Software; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods; D017356:Synchrotrons / Q000295:instrumentation
D011506:Proteins