title: Eco1524I, a type II restriction endonuclease: isolation, partial purification, and characterization.
abstract: Various strains of Escherichia coli, isolated from different patients, were screened for type II restriction endonuclease activity. In 1 out of 23 patients, a type II restriction endonuclease activity was found. The restriction endonuclease designated Eco1524I was purified to near homogeneity, based on hydroxyapatite and heparin sepharose chromatography. Eco1524I exhibited endonuclease restriction activity in the pH range from 6.0 to 10.0 (maximum level at pH 8.0) and required Mg2+ as divalent cation. The enzyme was stable till temperature 55 degrees C and pH range from 6.0 to 10.0. Eco1524I recognized the sequence 6-bp palindromic 5'AGG downward arrow CCT 3', producing blunt end and is found to be an isoschizomer of Stu I.
authors.affiliation: Laboratoire de Biochimie, Faculté des Sciences de Tunis, Campus Universitaire 1060 Tunis, Tunisia.
authors.forename: Hadeer
authors.initials: H
authors.lastname: Lazim
authors.forename: Jytte
authors.initials: J
authors.lastname: Josephsen
authors.forename: Assia
authors.initials: A
authors.lastname: Ben Hassen
authors.forename: Omrane
authors.initials: O
authors.lastname: Belhadj
authors.forename: Férid
authors.initials: F
authors.lastname: Limam
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D013045:Species Specificity; D013696:Temperature
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific