Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum.
A lacto-N-biose phosphorylase (LNBP) was purified from the cell extract of Bifidobacterium bifidum. Its N-terminal and internal amino acid sequences were homologous with those of the hypothetical protein of Bifidobacterium longum NCC2705 encoded by the BL1641 gene. The homologous gene of the type strain B. longum JCM1217, lnpA, was expressed in Escherichia coli to confirm that it encoded LNBP. No significant identity was found with any proteins with known function, indicating that LNBP should be classified in a new family. The lnpA gene is located in a novel putative operon for galactose metabolism that does not contain a galactokinase gene. The operon seems to be involved in intestinal colonization by bifidobacteria mediated by metabolism of mucin sugars. In addition, it may also resolve the question of the nature of the bifidus factor in human milk as the lacto-N-biose structure found in milk oligosaccharides.
Kitaoka
Motomitsu
M
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. mkitaoka@nfri.affrc.go.jp
Tian
Jiesheng
J
Nishimoto
Mamoru
M
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D005690:Galactose / Q000378:metabolism*; D008892:Milk / Q000737:chemistry; D009876:Operon; D010204:Pantetheine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006005:Phosphorylases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment; D013379:Substrate Specificity
D001426:Bacterial Proteins; D010204:Pantetheine; C033398:galactosyl-1,3-N-acetylglucosamine; C000354:bifidus factor; D006005:Phosphorylases; D000117:Acetylglucosamine; D005690:Galactose