Action of a novel pyrrolo[1,2-c][1.3]benzodiazepine on the viability of Jurkat and neuronal/glial cells.
We have designed and synthesized several structural isomers of anthramycin (heterocycles 2, 3, 5, 6, and 8) and found that, in particular, pyrrolobenzodiazepine 8 induces DNA cleavage and formation of small fragments of DNA. The cytotoxic effects of 8 were manifested with both non-transformed primary neuronal/glial cells and transformed Jurkat cells. The other compounds did not change the viability either of transformed or of non-transformed cells, and induced DNA cleavage to a lesser extent.
Rotas
Georgios
G
Department of Chemistry, University of Ioannina, GR-451 10 Ioannina, Greece.
Natchkebia
Ketevan
K
Natsvlishvili
Nino
N
Kekelidze
Merab
M
Kimbaris
Athanasios
A
Varvounis
George
G
Mikeladze
David
D
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001569:Benzodiazepines / Q000138:chemical synthesis / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000188:drug therapy* / Q000473:pathology; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D001569:Benzodiazepines; D004247:DNA