title: Importance of polyunsaturated acyl chains in chlorpromazine interaction with phosphatidylserines: a 13C and 31P solid-state NMR study.
abstract: The polyunsaturated fatty acid docosahexaenoic acid (DHA, c22:6, n-3) is found at a level of about 50% in the phospholipids of neuronal tissue membranes and appears to be crucial to human health. Dipalmitoyl phosphatidylcholine (DPPC, 16:0/16:0 PC), 1-palmitoyl-2-oleoyl phosphatidylserine (POPS) and the DHA containing 1-stearaoyl-2-docosahexenoyl phosphatidylserine (SDPS) were used to make DPPC (60%)/POPS (29%)/SDPS (11%) bilayers with and without 10 mol% chlorpromazine (CPZ), a cationic, amphiphilic phenothiazine. The T1 relaxation measurements make it clear that the saturated acyl chains carbons (palmitic, stearic and most of the oleic chain) and the choline head group are not affected by CPZ addition. The observed increased signal intensity and T1-values of DHA indicate reduced mobility of C4 and C5 due to CPZ binding. 31P NMR spectra confirm that CPZ binding to the phosphatidylserines in the bilayer enhances phospholipid head group mobility.
authors.affiliation: Department of Chemistry, University of Bergen, Allegaten 41, N-5007 Bergen, Norway.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.forename: Anja Underhaug
authors.initials: AU
authors.lastname: Gjerde
authors.forename: Holm
authors.initials: H
authors.lastname: Holmsen
authors.forename: Willy
authors.initials: W
authors.lastname: Nerdal
mesh_terms: D002247:Carbon Isotopes; D002746:Chlorpromazine / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D015394:Molecular Structure; D010718:Phosphatidylserines / Q000737:chemistry*; D010759:Phosphorus Isotopes
chemical_list: D002247:Carbon Isotopes; D008051:Lipid Bilayers; D010718:Phosphatidylserines; D010759:Phosphorus Isotopes; D004281:Docosahexaenoic Acids; D002746:Chlorpromazine