Hyaluronan-binding motif identified by panning a random peptide display library.
The glycosaminoglycan hyaluronan (HA) is involved in a variety of functions such as cell migration, adhesion, activation of intracellular signaling, metastasis, inflammation and wound repair. These functions of HA are mediated via HA-binding proteins (HABPs). To derive details of the HA-binding site in HABPs, here, we panned a random peptide display library expressed on the E. coli flagellin protein using HA-coated plates. Using this random peptide display library, 40 positive clones were obtained and the nucleotide sequences were determined. As a result, an Arg-Arg sequence, in addition to the known B-X7-B motif, was found to bind to HA. A binding experiment using the IAsys resonant mirror biosensor verified that a peptide containing an Arg-Arg sequence binds to HA.
Amemiya
Kana
K
Department of Biochemistry, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Nakatani
Tatsuya
T
Saito
Akio
A
Suzuki
Atsuo
A
Munakata
Hiroshi
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000235:genetics; D005408:Flagellin / Q000096:biosynthesis / Q000235:genetics; D006820:Hyaluronic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins; D005408:Flagellin; D006820:Hyaluronic Acid; D001120:Arginine