title: Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance.
abstract: A rat model for human minimal change nephropathy was obtained by the intravenous injection of adriamycin (ADR) at 5 mg/kg. By using an in vivo electron paramagnetic resonance (EPR) spectrometer operating at 700 MHz, the temporal changes in signal intensities of a nitroxide radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), in the kidneys of rats with ADR nephropathy were investigated. The decay rate of the EPR signal intensity obtained in the kidney is indicative of the renal reducing ability. It was found that the reducing ability in the kidney declined on the 7th day after ADR administration and recovered after the 14th day. Impairment of the reducing ability occurred before the appearance of continuous urinary protein. The in vitro EPR study showed that this impairment of in vivo renal reducing ability is related to impairment of the reducing ability in the mitochondria.
authors.affiliation: Pathophysiology of Renal Diseases, Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Oteki
authors.forename: Sohji
authors.initials: S
authors.lastname: Nagase
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohya
authors.forename: Takao
authors.initials: T
authors.lastname: Akatsuka
authors.forename: Mika
authors.initials: M
authors.lastname: Tada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ueda
authors.forename: Aki
authors.initials: A
authors.lastname: Hirayama
authors.forename: Akio
authors.initials: A
authors.lastname: Koyama
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004317:Doxorubicin; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016166:Free Radical Scavengers; D004317:Doxorubicin