Effect of surface attachment on synthesis of bacterial cellulose.
Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations optimized for maximal cellulose production rates. The behavior of these bacteria on semisolid and solid surfaces has not been specifically addressed. The species Gluconacetobacter hansenii was examined for cellulose synthesis and colony morphology on a range of solid supports, including cotton linters, and on media thickened with agar, methyl cellulose, or gellan. The concentration and chemical structure of the thickening agent were found to be directly related to the formation of contiguous cellulose pellicules. Viability of the bacteria following freezer storage was improved when the bacteria were frozen in their cellulose pellicules.
Evans
Barbara R
BR
Chemical Sciences Division, Oak Ridge National Laboratory, 4500N Bethel Valley Road, Oak Ridge, TN 37831-6194, USA. evansb@ornl.gov
O'Neill
Hugh M
HM
D001422:Bacterial Adhesion / Q000502:physiology*; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002482:Cellulose / Q000096:biosynthesis*; D015925:Cryopreservation / Q000379:methods*; D041821:Gluconacetobacter / Q000145:classification / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D008567:Membranes, Artificial; D013499:Surface Properties
D008567:Membranes, Artificial; D002482:Cellulose