title: Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line.
abstract: Mosquito densoviruses generally establish persistent infections in mosquito cell lines including the C6/36 Aedes albopictus cell line. In contrast, the closely related Haemagogus equinus densovirus (HeDNV) causes dramatic cytopathic effects in the C6/36 Aedes albopictus cell line. Infection of C6/36 cells by HeDNV causes internucleosomal fragmentation of host chromosomal DNA, changes in cellular morphology (membrane budding, apoptotic bodies), caspase activation and exposure of phosphatidylserine on the cellular membrane. This is accompanied by a higher rate of infection and more vigorous production of virus in these cells. These observations are consistent with the induction of apoptosis during infection. In contrast, expression of AeDNV proteins in C6/36 cells does not cause obvious cytopathic effects although NS1 expression causes accumulation of cells in G2 phase. C6/36 cells persistently infected with AeDNV were not protected from superinfection with HeDNV. Thus, there does not seem to be an antiviral state induced by AeDNV persistent infection.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Paterson
authors.forename: Erin
authors.initials: E
authors.lastname: Robinson
authors.forename: Erica
authors.initials: E
authors.lastname: Suchman
authors.forename: Boris
authors.initials: B
authors.lastname: Afanasiev
authors.forename: Jonathan
authors.initials: J
authors.lastname: Carlson
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line / Q000473:pathology / Q000821:virology; D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019223:Densovirinae / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007700:Kinetics; D013997:Time Factors; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D004247:DNA