title: A media design program for lactic acid production coupled with extraction by electrodialysis.
abstract: The aim of this study was to investigate industrial media for lactic acid fermentation to reduce the cost of nitrogen sources. Corn steep liquor (CSL) was successfully used at 5% (v/v) in batch fermentations. Use of soluble CSL improved the productivity approximately 20% with an advantage of clearer fermentation broth. Yeast extract (YE)-complemented CSL media further increased the productivity. It was found that 3.1 g L(-1) yeast extract and 5% CSL could be an effective substitute for 15 g L(-1) yeast extract in 10% glucose medium. Spent brewery yeast was also used as a sole nitrogen source equivalent to 5% CSL. Lactic acid was recovered by electrodialysis from the cell free broth. Depleted cell free broth supplemented with 5 g L(-1) of yeast extract performed reasonably in batch cultures. Reuse of the fermentation broth may reduce the cost of raw materials as well as minimize the fermentation wastes.
authors.affiliation: Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), South Korea. klee@wistar.upenn.edu
authors.forename: KiBeom
authors.initials: K
authors.lastname: Lee
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis / Q000379:methods; D005285:Fermentation; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013053:Spectrophotometry; D003313:Zea mays / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D019344:Lactic Acid; D005947:Glucose