title: Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans.
abstract: An actinomycetes expression vector (pIBR25) was constructed and applied to express a gene from the kanamycin biosynthetic gene cluster encoding 2-deoxy-scyllo-inosose synthase (kanA) in Streptomyces lividans TK24. The expression of kanA in pIBR25 transformants reached a maximum after 72 h of culture. The plasmid pIBR25 showed better expression than pSET152, and resulted in the formation of insoluble KanA when it was expressed in Escherichia coli. This strategy thus provides a valuable tool for expressing aminoglycoside-aminocyclitols (AmAcs) biosynthetic genes in Streptomyces spp.
authors.affiliation: Institute of Biomolecule Reconstruction, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, Korea.
authors.forename: Mai Le
authors.initials: ML
authors.lastname: Thuy
authors.forename: Madan Kumar
authors.initials: MK
authors.lastname: Kharel
authors.forename: Rajan
authors.initials: R
authors.lastname: Lamichhane
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D015870:Gene Expression; D005822:Genetic Vectors; D007612:Kanamycin / Q000096:biosynthesis; D008190:Lyases / Q000096:biosynthesis* / Q000235:genetics*; D048170:Streptomyces lividans / Q000201:enzymology* / Q000235:genetics*
chemical_list: D007612:Kanamycin; D008190:Lyases; C085631:2-deoxy-scyllo-inosose synthase