Ornithine decarboxylase, mitogen-activated protein kinase and matrix metalloproteinase-2 expressions in human colon tumors.
OBJECTIVE
To investigate the expressions of ornithine decarboxylase (ODC), MMP-2, and Erk, and their relationship in human colon tumors.


METHODS
ODC activity, MMP-2 expression, and mitogen-activated protein (MAP) kinase activity (Erk phosphorylation) were determined in 58 surgically removed human colon tumors and their adjacent normal tissues, using [1-14C]-ornithine as a substrate, ELISA assay, and Western blotting, respectively.


RESULTS
ODC activity, MMP-2 expression, and Erk phosphorylation were significantly elevated in colon tumors, compared to those in adjacent normal tissues. A significant correlation was observed between ODC activities and MMP-2 levels.


CONCLUSIONS
This is the first report showing a significant correlation between ODC activities and MMP-2 levels in human colon tumors. As MMP-2 is involved in cancer invasion and metastasis, and colon cancer overexpresses ODC, suppression of ODC expression may be a rational approach to treat colon cancer which overexpresses ODC.
Nemoto
Takahiro
T
Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
Kubota
Shunichiro
S
Ishida
Hideyuki
H
Murata
Nobuo
N
Hashimoto
Daijo
D
D000970:Antineoplastic Agents / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000201:enzymology*; D000518:Eflornithine / Q000627:therapeutic use; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D065108:Ornithine Decarboxylase Inhibitors
D000970:Antineoplastic Agents; D065108:Ornithine Decarboxylase Inhibitors; D020928:Mitogen-Activated Protein Kinases; D020778:Matrix Metalloproteinase 2; D009955:Ornithine Decarboxylase; D000518:Eflornithine