Animal models and analytical approaches for understanding the relationships between wine and cancer.
We used two approaches for studying the relationships between wine consumption, wine composition and cancer In the first approach, a transgenic mouse model of human neurofibromatosis, combined with the use of well-defined, chemically purified diets, showed that red wine contains nonalcoholic components that can delay tumor onset. In additional studies, catechin, the main monomeric polyphenol of red wine, delayed tumor onset in this mouse model in a positive, linear relationship when incorporated into the diet at levels of 0.5-4 mmol/kg diet. In the second approach, low doses of the chemical carcinogen 2-amino-1-methyl-6-phenylimidazo(4, 5-b)pyridine (PhlP) were administered to rats, and formation of DNA adducts was evaluated by accelerator mass spectrometry. Consumption of red wine solids (the residue from red wine remaining after removal of alcohol and water) and the wine polyphenol quercetin did not influence PhlP-DNA adduct levels or induce liver enzymes (glutathione-S-transferase and quinone reductase). However, quercetin did alter distribution of PhlP in the rat tissues compared to control animals and animals fed other potential dietary chemopreventive agents, including phenylethyl isothiocyanate and sulforaphane. These studies demonstrate the feasibility of these approaches for studying the chemopreventive potential of dietary components at physiologic levels in
Ebeler
S E
SE
Department of Viticulture and Enology, University of California, Davis, CA 95616, USA. seebeler@ucdavis.edu
Dingley
K H
KH
Ubick
E
E
Abel
S
S
Mitchell
A E
AE
Burns
S A
SA
Steinberg
F M
FM
Clifford
A J
AJ
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D002392:Catechin / Q000494:pharmacology; D018736:DNA Adducts / Q000096:biosynthesis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007093:Imidazoles / Q000378:metabolism / Q000633:toxicity*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D017253:Neurofibromatoses / Q000235:genetics / Q000517:prevention & control*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D027843:Vitis / Q000737:chemistry; D014920:Wine
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D018736:DNA Adducts; D007093:Imidazoles; D002392:Catechin; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D011794:Quercetin