Using the flow cytometry to quantify the Giardia cysts and Cryptosporidium oocysts in water samples.
The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FL1 versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and Waterborne Inc.
Hsu
Bing-Mu
BM
Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chung-Li, Taiwan, ROC. bmhsu@mail.yust.edu.tw
Wu
Nan-Min
NM
Jang
Hung-Der
HD
Shih
Feng-Cheng
FC
Wan
Min-Tao
MT
Kung
Chien-Min
CM
D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct; D005872:Giardia / Q000302:isolation & purification*; D033661:Oocysts / Q000302:isolation & purification*; D014867:Water / Q000469:parasitology*
D014867:Water