[Protective effect of ischemic preconditioning duration against ischemia-reperfusion injury of skeletal muscle in rats].
OBJECTIVE
To study the protective effects of ischemic preconditioning (IP) duration against ischemic reperfusion injury of skeletal muscle.


METHODS
Thirty-six Wistar rats were made amputation-like models, which underwent temporary amputation at the level of the femur, excluding the femoral vessels. They were divided into 6 groups (n=6) according to different treatments before ischemia-reperfusion: group A (4 hours of ischemia-reperfusion); groups B, C, D, E (5, 10, 15, 20 minutes of ischemia and 5, 10, 15, 20 minutes of reperfusion respectively, for 3 cycles, 4 hours ischemia-reperfusion); group F (no ischemia-reperfusion). The malondialdehyde (MDA), the extent of edema and necrosis of skeletal muscle were measured to observe protective effects of different ischemic preconditioning duration.


RESULTS
Five minutes of ischemic preconditioning (IP5) could protect skeletal muscle of ischaemia against necrosis and the survival area of the muscle was 82.47%. The effects of IP10 and IP15 were significantly superior to that of IP5 and the survival areas of the muscle were 89.03% and 89.49%. The effect of IP20 (78.27%) was significantly inferior to that of IP5. IP5 could reduce edema of skeletal muscle, the effect of IP10 was significantly superior to that of IP5. IP5, IP10, and IP15 could decrease the level of MDA, but IP20 did not decrease it.


CONCLUSIONS
The trend of protective effect of IP on ischemia-reperfusion injury of the muscle in rats first rise to the peak and then go down, 10 minutes of IP is optimal.
Qi
Wei
W
Department of Hand Surgery, Tianjin Hospital, Tianjin, China. qxcs8@hotmail.com
Kan
Shi Lian
SL
D000818:Animals; D004195:Disease Models, Animal; D019194:Ischemic Preconditioning; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control; D013997:Time Factors