Immunohistological characterization of macrophage migration inhibitory factor expression in Plasmodium falciparum-infected placentas.
Previously, we have shown that macrophage migration inhibitory factor (MIF) was highly elevated in the placental intervillous blood (IVB) of Plasmodium falciparum-infected women. Here, we compared the expression of MIF in placental tissues obtained from P. falciparum-infected and -uninfected women. Immunoperoxidase staining showed a consistent pattern of MIF expression in syncytiotrophoblasts, extravillous trophoblasts, IVB mononuclear cells, and amniotic epithelial cells, irrespective of their malaria infection status. Cytotrophoblast, villous stroma, and Hofbauer cells showed focal staining. Only amniotic epithelial and IVB mononuclear cells from P. falciparum-infected placentas exhibited significantly higher level of MIF expression than uninfected placentas. Stimulation of syncytilized human trophoblast BeWo cells with P. falciparum-infected erythrocytes that were selected to bind these cells resulted in significant increases in MIF secretion, whereas control erythrocytes, lipopolysaccharides, and synthetic beta-hematin had minimal effect. These findings suggest that placental malaria modulates MIF expression in different placental compartments.
Chaisavaneeyakorn
Sujittra
S
Centers for Disease Control and Prevention, 4770 Buford Highway, Mail Stop F-12, Chamblee, GA 30341, USA.
Lucchi
Naomi
N
Abramowsky
Carlos
C
Othoro
Caroline
C
Chaiyaroj
Sansanee C
SC
Shi
Ya Ping
YP
Nahlen
Bernard L
BL
Peterson
David S
DS
Moore
Julie M
JM
Udhayakumar
Venkatachalam
V
D000818:Animals; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis* / Q000096:biosynthesis; D010920:Placenta / Q000737:chemistry* / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000472:pathogenicity*; D011247:Pregnancy
D008263:Macrophage Migration-Inhibitory Factors