Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells.
OBJECTIVE
Some monoclonal antibodies (mAb) to type II collagen (CII) are arthritogenic upon passive transfer to mice. We undertook this study to investigate whether such mAb are pathogenic in the absence of mediators of inflammation.


METHODS
The arthritogenic mAb CIIC1 and M2139, and the nonarthritogenic mAb CIIF4, each reactive with a distinct and well-defined conformational epitope on CII, were compared with control mAb GAD6. Bovine chondrocytes were cultured with one of the mAb, and on days 3, 6, and 9, antibody binding by chondrocytes and newly synthesized extracellular matrix (ECM) was examined by immunofluorescence, morphologic effects were studied by electron microscopy, and synthesis of matrix components was determined by metabolic labeling with (3)H-proline for collagen and (35)S-sulfate for proteoglycans.


RESULTS
All 3 mAb to CII bound to the matrix. CIIC1 and M2139 adversely affected the cultures, whereas CIIF4 did not. CIIC1 caused disorganization of CII fibrils in the ECM without affecting chondrocyte morphology, and increased matrix synthesis. M2139 caused thickening and aggregation of CII fibrils in the ECM and abnormal chondrocyte morphology but matrix synthesis was unaffected.


CONCLUSIONS
The unique arthritogenic capacity of particular anti-CII mAb upon passive transfer could be explained by their adverse, albeit differing, effects in primary cultures of chondrocytes. Such effects occur independent of inflammation mediators and are related to the epitope specificity of the mAb. Interference with the structural integrity of CII could precede, and even initiate, the inflammatory expression of disease.
Amirahmadi
S Fatemah
SF
Monash University, Clayton, Victoria, Australia.
Whittingham
Senga
S
Crombie
Duncan E
DE
Nandakumar
Kutty Selva
KS
Holmdahl
Rikard
R
Mackay
Ian R
IR
van Damme
Marie-Paule
MP
Rowley
Merrill J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001168:Arthritis / Q000276:immunology*; D015415:Biomarkers; D002356:Cartilage / Q000276:immunology; D019902:Chondrocytes / Q000276:immunology*; D024043:Collagen Type II / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D024043:Collagen Type II