title: Characterization of four terpene synthase cDNAs from methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii.
abstract: Numerous terpenoid compounds are present in copious amounts in the oleoresin produced by conifers, especially following exposure to insect or fungal pests. CDNA clones for many terpene synthases responsible for the biosynthesis of these defense compounds have been recovered from several conifer species. Here, the use of three terpene synthase sequences as heterologous probes for the discovery of related terpene synthase genes in Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco (Pinaceae), is reported. Four full-length terpene synthase cDNAs were recovered from a methyl jasmonate-induced Douglas-fir bark and shoot cDNA library. These clones encode two multi-product monoterpene synthases [a (-)-alpha-pinene/(-)-camphene synthase and a terpinolene synthase] and two single-product sesquiterpene synthases [an (E)-beta-farnesene synthase and a (E)-gamma-bisabolene synthase].
authors.affiliation: Michael Smith Laboratories and Departments of Botany and Forest Sciences, University of British Columbia, #301 - 2185 East Mall, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Dezene P W
authors.initials: DP
authors.lastname: Huber
authors.forename: Ryan N
authors.initials: RN
authors.lastname: Philippe
authors.forename: Kimberley-Ann
authors.initials: KA
authors.lastname: Godard
authors.forename: Rona N
authors.initials: RN
authors.lastname: Sturrock
authors.forename: Jörg
authors.initials: J
authors.lastname: Bohlmann
mesh_terms: D000085:Acetates; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003517:Cyclopentanes; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D054883:Oxylipins; D024301:Plant Bark / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010937:Plant Growth Regulators; D010940:Plant Proteins / Q000235:genetics*; D018520:Plant Shoots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D028224:Pseudotsuga / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D018744:DNA, Plant; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013729:Terpenes; C072239:methyl jasmonate; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase