title: Fungal endophyte N-acetylglucosaminidase expression in the infected host grass.
abstract: Fungal endophytes of the genera Neotyphodium and Epichlolë are important mutualistic symbionts and pathogens of many cool-season grass species. Here we report the characterization of a secreted N-acetylglucosaminidase from the Neotyphodium sp. endophyte that infects the grass Poa ampla. The enzyme was expressed at low levels within the host, and activity could be detected in the apoplastic protein fraction. Low-level expression could also be detected in endophyte-infected perennial ryegrass (Lolium perenne), Chewings fescue (Festuca rubra subsp. fallax), and tall fescue (L. arundinaceum). The enzyme may function in the recycling of chitin oligomers generated from turnover of the fungal cell wall. This is the first report of a secreted N-acetylglucosaminidase expressed by an endophytic fungus in the infected host plant.
authors.affiliation: Department of Plant Biology and Pathology, Cook College, Rutgers University,, 59 Dudley Road, New Brunswick, NJ 08903, USA.
authors.forename: Huaijun Michael
authors.initials: HM
authors.lastname: Li
authors.forename: Jo Anne
authors.initials: JA
authors.lastname: Crouch
authors.forename: Faith C
authors.initials: FC
authors.lastname: Belanger
mesh_terms: D000118:Acetylglucosaminidase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D015971:Gene Expression Regulation, Enzymologic; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D006109:Poaceae / Q000382:microbiology*
chemical_list: D000118:Acetylglucosaminidase