[Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase].
OBJECTIVE
To investigate the relationship between DNA degradeation and postmortem interval.


METHODS
dUTP was transferred to 3'terminal of DNA by using terminal deoxynucleotidyl transferase (TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected.


RESULTS
The reminders of dUTP were decreasing along with the postmortem interval.


CONCLUSIONS
Postmortem DNA degradation may be used in postmortem interval judgment.
Zhan
Lan
L
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhao
Zi-qin
ZQ
Shan
Yi-wen
YW
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004796:Clinical Enzyme Tests / Q000379:methods; D004247:DNA / Q000378:metabolism*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D011180:Postmortem Changes; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000378:metabolism; D013997:Time Factors
D004247:DNA; D004253:DNA Nucleotidylexotransferase; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase