title: Chicken liver bile acid-binding protein is in a compact partly folded state at acidic pH. Its relevance to the interaction with lipid membranes.
abstract: Chicken liver bile acid-binding protein (formerly known as chicken liver basic fatty acid-binding protein) binds to anionic lipid membranes acquiring a partly folded state [Nolan, V., Perduca, M., Monaco, H., Maggio, B., and Montich, G. (2003) Biochim. Biophys. Acta 1611, 98-106]. To understand the mechanisms of its interactions with membranes, we have investigated the presence of partly folded states in solution. Using fluorescence spectroscopy of the single Trp residue, circular dichroism in the far- and near-UV, Fourier transform infrared spectroscopy, and size-exclusion chromatography, we found that L-BABP was partly unfolded at pH 2.5 and low ionic strength, retaining some of its secondary structure. Addition of 0.1 M NaCl at pH 2.5 or decreasing the pH to 1.5 produced a more compact partly folded state, with a partial increase of secondary structure and none of tertiary structure. Fluorescence emission spectra of this state indicate that the Trp residue is within an environment of low polarity, similar to the native state. This environment is not produced by the insertion of the Trp into soluble aggregates as revealed by size-exclusion chromatography, fluorescence anisotropy, and infrared spectroscopy. The presence of partly folded states under acidic conditions in solution suggests the possibility that membrane binding of L-BABP occurs via this state.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba - CIQUIBIC (CONICET) - Pabellón Argentina, Ciudad Universitaria (5000) Córdoba, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Nolan
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Perduca
authors.forename: Hugo L
authors.initials: HL
authors.lastname: Monaco
authors.forename: Guillermo G
authors.initials: GG
authors.lastname: Montich
mesh_terms: D000143:Acids / Q000737:chemistry; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002645:Chickens; D002850:Chromatography, Gel; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism*; D009994:Osmolar Concentration; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012996:Solutions; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000143:Acids; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008563:Membrane Lipids; D012996:Solutions; C037643:bile acid binding proteins