title: responses of mycobacterium tuberculosis to growth in the mouse lung.
abstract: Using a promoter trap, we have identified 56 Mycobacterium tuberculosis genes preferentially expressed in the mouse lung. Quantitative real-time PCR showed that RNA levels of several genes were higher from bacteria growing in mouse lungs than from broth cultures. These results support the current hypothesis that Mycobacterium tuberculosis utilizes fatty acids as a carbon source in the mouse lung.
authors.affiliation: The Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
authors.forename: Eugenie
authors.initials: E
authors.lastname: Dubnau
authors.forename: John
authors.initials: J
authors.lastname: Chan
authors.forename: V P
authors.initials: VP
authors.lastname: Mohan
authors.forename: Issar
authors.initials: I
authors.lastname: Smith
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins; D024881:Drug Resistance, Bacterial; D007538:Isoniazid / Q000494:pharmacology; D008168:Lung / Q000382:microbiology*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000254:growth & development*; D010088:Oxidoreductases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D010088:Oxidoreductases; C085257:InhA protein, Mycobacterium; D007538:Isoniazid