Determination of oxytetracycline by flow injection with chemiluminescence detection.
A flow-injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2'-bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2'-bipyridine) ruthenium (II) by cerium (IV) sulphate in sulphuric acid. Under the optimum conditions, the calibration curve was linear over the range 1.0 x 10(-7)-1.0 x 10(-5) g/mL for oxytetracycline with the linear equation: DeltaINT = 148.77 x C + 0.6637 (R2 = 0.9994). The detection limit was 4.52 x 10(-8) g/mL. The proposed method was also successfully used to determine oxytetracycline in pharmaceutical formulations. The mean recovery of determination of oxytetracycline was 92.73%. A mechanism for the chemiluminescence enhancement by oxytetracycline of tris(2,2'-bipyridine)-ruthenium (II) and cerium (IV) sulphate system is also proposed.
Guo
Liangqia
L
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.
Xie
Zenghong
Z
Bian
Xintong
X
Lin
Xucong
X
Zhang
Weilin
W
Liu
Xiaohua
X
Chen
Guonan
G
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D002138:Calibration; D056831:Coordination Complexes; D008163:Luminescent Measurements / Q000379:methods / Q000592:standards; D010084:Oxidation-Reduction; D010118:Oxytetracycline / Q000032:analysis* / Q000592:standards; D004364:Pharmaceutical Preparations / Q000032:analysis; D013464:Sulfuric Acids; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D010118:Oxytetracycline; D004364:Pharmaceutical Preparations; D013464:Sulfuric Acids; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C033158:sulfuric acid