title: OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation.
abstract: The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified. The expression of OsLSD1 was light-induced or dark-suppressed. Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants. Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1 mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus. Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus. Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin. OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells. Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.
authors.affiliation: National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Zhongyou
authors.initials: Z
authors.lastname: Pei
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Chaozu
authors.initials: C
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D002734:Chlorophyll / Q000096:biosynthesis; D037341:Fumonisins / Q000633:toxicity; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000502:physiology*; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D037341:Fumonisins; D010940:Plant Proteins; D002734:Chlorophyll; C056933:fumonisin B1; C037184:chlorophyll b