title: Experimental model of pulmonary metastasis treatment with IFNalpha.
abstract: Melanoma shows the high rates of mortality, preferentially by metastasis, and the failure of chemotherapy. We inoculated 30 B16F10 Swiss mice with 5 x 10(5) melanocytes. The mice were then inoculated with 150,000; 300,000; 600,000 and 1,200,000 IU/mouse of interferon alpha. A count of the metastatic nodules of the lung surface and the microscopic study were made by image analysis on five histological sections, calculating the implantation percentage and tumoral growth and invasion index. Treatment with the three highest doses of interferon alpha resulted in a dose-dependent reduction of the number of metastatic nodules and invaded area. A correlation existing between the parameters studied and the different antitumoral effects observed.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain. crismart@um.es
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente
authors.forename: M Josefa
authors.initials: MJ
authors.lastname: Yáñez
authors.forename: Juana M
authors.initials: JM
authors.lastname: García
authors.forename: Manuel
authors.initials: M
authors.lastname: Canteras
authors.forename: Miguel
authors.initials: M
authors.lastname: Alcaraz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary* / Q000662:veterinary; D008544:Melanocytes; D008545:Melanoma / Q000188:drug therapy* / Q000556:secondary* / Q000662:veterinary; D051379:Mice; D009374:Neoplasms, Experimental; D012878:Skin Neoplasms / Q000473:pathology* / Q000662:veterinary; D014183:Transplantation, Heterologous
chemical_list: D000970:Antineoplastic Agents; D016898:Interferon-alpha