title: Characterization of markers to determine the extent and variability of leaf senescence in Arabidopsis. A metabolic profiling approach.
abstract: Comparison of the extent of leaf senescence depending on the genetic background of different recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana) is described. Five RILs of the Bay-0 x Shahdara population showing differential leaf senescence phenotypes (from early senescing to late senescing) were selected to determine metabolic markers to discriminate Arabidopsis lines on the basis of senescence-dependent changes in metabolism. The proportion of gamma-aminobutyric acid, leucine, isoleucine, aspartate, and glutamate correlated with (1) the age and (2) the senescence phenotype of the RILs. Differences were observed in the glycine/serine ratio even before any senescence symptoms could be detected in the rosettes. This could be used as predictive indicator for plant senescence behavior. Surprisingly, late-senescing lines appeared to mobilize glutamine, asparagine, and sulfate more efficiently than early-senescing lines. The physiological basis of the relationship between leaf senescence and flowering time was analyzed.
authors.affiliation: Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, 78 026 Versailles cedex, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Diaz
authors.forename: Sarah
authors.initials: S
authors.lastname: Purdy
authors.forename: Aurélie
authors.initials: A
authors.lastname: Christ
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Morot-Gaudry
authors.forename: Astrid
authors.initials: A
authors.lastname: Wingler
authors.forename: Céline
authors.initials: C
authors.lastname: Masclaux-Daubresse
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003433:Crosses, Genetic; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism / Q000502:physiology*
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D002734:Chlorophyll; D002244:Carbon; D009584:Nitrogen