title: Identification of a novel gene family, paralogs of inhibitor of apoptosis proteins present in plants, fungi, and animals.
abstract: Only few orthologs of animal apoptosis regulators have been found in plants. Recently, the ectopic expression of mammalian inhibitor of apoptosis proteins (IAPs) has been shown to affect plant programmed cell death. Here, we identified two novel proteins homologous to Arabidopsis thaliana IAP-like protein (AtILP) 1 and 2 by applying an improved motif searching method. Furthermore, homologs of AtILP1 were found to occur as a novel gene family in other organisms such as fungi and animals including Homo sapiens (HsILP1). Like baculovirus IAP repeats (BIRs) in IAPs, ILPs contain two highly conserved BIR-like domains (BLDs) with a putative C2HC-type zinc finger. Phylogenetic analyses indicated that ILPs are putative paralogs of IAPs. Homology modeling revealed that the three-dimensional structure of BLD in HsILP1 is similar to that of BIR. Transient expression of HsILP1 resulted in inhibition of etoposide-induced apoptosis in HEK293 and HeLaS3 cells. These findings suggest that ILPs are conserved in a wide range of eukaryotes including plants, and that their functions are closely related to those of IAPs.
authors.affiliation: Genome and Drug Research Center, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Higashi
authors.forename: R
authors.initials: R
authors.lastname: Takasawa
authors.forename: A
authors.initials: A
authors.lastname: Yoshimori
authors.forename: T
authors.initials: T
authors.lastname: Goh
authors.forename: S
authors.initials: S
authors.lastname: Tanuma
authors.forename: K
authors.initials: K
authors.lastname: Kuchitsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D017360:Arabidopsis / Q000235:genetics*; D002460:Cell Line; D005047:Etoposide / Q000494:pharmacology; D005800:Genes, Fungal; D017343:Genes, Plant; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D011506:Proteins; D005047:Etoposide