title: An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus.
abstract: Two forms of acetyl-CoA synthetase (ACS1 and ACS2) have been detected in Phycomyces blakesleeanus. ACS1, encoded by the gene facA, was induced by acetate and repressed by glucose at the transcriptional level. ACS2, not encoded by the gene facA, was detected as a response to carbon starvation both in the wild type and in an facA(-) mutant. Both enzymes were purified and characterized. They can use acetate and propionate as substrates. ACS2 is a much more stable enzyme than ACS1. After 60 min incubation at 55 degrees C, ACS2 retained 50% of its activity whereas ACS1 only retained 3%. The optimum temperature was 50 degrees C for ACS2 and 30 degrees C for ACS1.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24007 León, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: De Cima
authors.forename: Javier
authors.initials: J
authors.lastname: Rúa
authors.forename: Eusebio
authors.initials: E
authors.lastname: Perdiguero
authors.forename: Pilar
authors.initials: P
authors.lastname: del Valle
authors.forename: Félix
authors.initials: F
authors.lastname: Busto
authors.forename: Alberto
authors.initials: A
authors.lastname: Baroja-Mazo
authors.forename: Dolores
authors.initials: D
authors.lastname: de Arriaga
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D015152:Blotting, Northern; D003066:Coenzyme A Ligases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004795:Enzyme Stability; D015966:Gene Expression Regulation, Fungal; D006358:Hot Temperature; D007700:Kinetics; D010800:Phycomyces / Q000201:enzymology*; D011422:Propionates / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D011422:Propionates; D012331:RNA, Fungal; D012333:RNA, Messenger; D003066:Coenzyme A Ligases; C101958:acetate-CoA ligase (ADP-forming); C029658:propionic acid; D019342:Acetic Acid