EEDA: a protein associated with an early stage of stratified epithelial differentiation.
Using suppressive subtractive hybridization, we have identified a novel gene, which we named early epithelial differentiation associated (EEDA), which is uniquely associated with an early stage of stratified epithelial differentiation. In epidermis, esophageal epithelium, and tongue epithelium, EEDA mRNA, and antigen was abundant in suprabasal cells, but was barely detectable in more differentiated cells. Consistent with the limbal location of corneal epithelial stem cells, EEDA was expressed in basal corneal epithelial cells that are out of the stem cell compartment, as well as the suprabasal corneal epithelial cells. The strongest EEDA expression occurred in suprabasal precortical cells of mouse, bovine, and human anagen follicles. Developmental studies showed that the appearance of EEDA in embryonic mouse epidermis (E 15.5) coincided with morphological keratinization. Interestingly, EEDA expression is turned off when epithelia were perturbed by wounding and by cultivation under both low and high Ca2+ conditions. Our results indicate that EEDA is involved in the early stages of normal epithelial differentiation, and that EEDA is important for the "normal" differentiation pathway in a wide range of stratified epithelia.
Sun
Lijie
L
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
Ryan
David G
DG
Zhou
Mingyuan
M
Sun
Tung-Tien
TT
Lavker
Robert M
RM
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003315:Cornea / Q000033:anatomy & histology; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D004848:Epithelium / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014018:Tissue Distribution
D051017:Apoptosis Regulatory Proteins; C091572:DAP protein, human; D011506:Proteins; C508722:early epithelial differentiation-associated protein, mouse