Expression profile analysis of human peripheral blood mononuclear cells in response to aspirin.
BACKGROUND
Aspirin is a popular nonsteroidal anti-inflammatory drug, but some patients suffer from hypersensitivity to it. This prompted us to identify the factors or molecules related to these responses.


METHODS
A commercially available DNA microarray was used to study changes in gene expression in human peripheral blood mononuclear cells (PBMCs) after aspirin treatment. The PBMCs were collected from a patient with aspirin-intolerant asthma and one normal healthy control.


RESULTS
We identified 61 and 107 genes respectively induced and repressed by aspirin treatment in the PBMCs derived from the normal control. In the patient showing aspirin-induced asthma responses, 31 genes were up-regulated and 6 were down-regulated after aspirin treatment. Among these,1 gene was expressed with the same pattern in the control and the patient. In contrast, 19 genes showed different expression patterns, and it turned out that most of them were involved in immune responses, cell growth/proliferation, transcription/translation, and signaling pathways.


CONCLUSIONS
These results show the molecules involved in hypersensitivity to aspirin and may lead to a better understanding of adverse responses to aspirin. Furthermore, they can provide clues for identifying novel therapeutic and/or preventive molecular targets of the adverse effects of aspirin.
Choi
Sunghee
S
College of Pharmacy, Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Women's University, Seoul, Korea.
Park
Hae-Sim
HS
Cheon
Myeong Sook
MS
Lee
Kyunglim
K
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000139:chemically induced / Q000235:genetics; D016022:Case-Control Studies; D018076:DNA, Complementary / Q000235:genetics; D004342:Drug Hypersensitivity / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018076:DNA, Complementary; D001241:Aspirin