[Influence of dermal template on the biomechanical compliance of wound tissue].
OBJECTIVE
To investigate the influence of dermal template on the biomechanical compliance of wound tissue during wound repair.


METHODS
One hundred and forty-four SD rats subjected to full-thickness skin loss on the dorsum were enrolled in the study, and they were randomly divided into A (n = 6, without grafting on wound), B (n = 6, with full thickness skin grafting on wound), C (n = 6, with razor thin skin grafting on wound) and D [n = 6, with acellular dermal matrix (ADM) and razor thin skin grafting on wound] groups. The tissue samples from the wounds were harvested 1, 2, 4, 6, 12 and 20 weeks after the operation. The biomechanical compliance of the wound was assessed by Instron biomechanics tensiometer. The expression of alpha-SMA in the dermal fibroblasts of each group was determined by immunohistochemistry (ABC) method.


RESULTS
The biomechanical compliance of the wound in D group was higher than that in A and C groups (P < 0.05), but lower than that in B group during 4 to 20 weeks after operation (P < 0.05). The expression of alpha-SMA in D group (7.53 +/- 0.98)% was lower than that in A (26.99 +/- 2.90)% and C (2.18 +/- 2.79)% groups (P < 0.01), but higher than that in B group at 4 weeks after operation (P < 0.05).


CONCLUSIONS
Dermal template might affect the scar formation during wound healing, in improving wound healing quality by enhancing the biomechanical compliance of wound tissue.
Liu
Ying-kai
YK
Department of Burns, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P.R. China.
Lu
Shu-liang
SL
Qing
Chun
C
Chen
Zhi-long
ZL
Xiang
Jun
J
Xie
Ting
T
Qiao
Liang
L
Lin
Wei-dong
WD
Hu
Qing-shen
QS
Liao
Zhen-jiang
ZJ
Shi
Ji-xiang
JX
D000199:Actins / Q000378:metabolism*; D000818:Animals; D003187:Compliance; D020405:Dermis / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016038:Skin Transplantation; D017768:Skin, Artificial; D017695:Soft Tissue Injuries / Q000503:physiopathology* / Q000601:surgery*; D014945:Wound Healing
D000199:Actins