title: Inflammatory mediators and eosinophilia in atopic and non-atopic patients with nasal polyposis.
abstract: Nasal polyps are characterized by eosinophilic infiltration and presence of inflammatory mediators, such as total IgE, eosinophil cationic protein (ECP) and cytokines. The role of atopy in nasal polyp pathogenesis is still unclear. Therefore, we evaluated serum IgE levels, nasal mucus concentrations of ECP and cytokines and the number of infiltrating eosinophils in nasal tissue of polyps from atopic and non-atopic patients. Samples were obtained from a randomized population of 31 patients with nasal polyposis having endonasal sinus surgery and of 13 control subjects undergone corrective surgery of the nasal septum. On the basis of medical history of allergy, positive skin-prick tests and total IgE levels, patients with polyposis were divided in atopic (n = 13) and non-atopic (n = 18) patients. We determined levels of IgE in blood, ECP and cytokines (IL-4, IL-6, IL-8, IFN-gamma and IL-2) in nasal mucus, and number of infiltrating eosinophils in nasal tissue. The concentrations of total IgE, ECP, IL-4 and IL-8 and eosinophilia were significantly higher in all patients with nasal polyps compared with controls. Inside, all patients with nasal polyposis showed lower levels of IL-6, IFN-gamma and IL-2 compared with controls. The atopic patients showed significant differences when compared with non-atopic patients for the higher concentrations of total IgE (698.80+/-322.24 vs. 279.63+/-234.11; P < 0.0001) and IL-8 (1437.2 pg/ml+/-1250.7 vs. 605.5 pg/ml+/-481.1; P < 0.015). These findings suggest that inflammation still remains the major factor in the etiology of nasal polyposis and show different levels of inflammatory mediators into atopic and non-atopic patients.
authors.affiliation: Dipartimento di Morfologia Umana e Biologia Applicata, Facoltà di Medicina e Chirurgia, Università di Pisa, Pisa, Italy. m.scavuzzo@med.unipi.it
authors.forename: M C
authors.initials: MC
authors.lastname: Scavuzzo
authors.forename: B
authors.initials: B
authors.lastname: Fattori
authors.forename: R
authors.initials: R
authors.lastname: Ruffoli
authors.forename: V
authors.initials: V
authors.lastname: Rocchi
authors.forename: A
authors.initials: A
authors.lastname: Carpi
authors.forename: R
authors.initials: R
authors.lastname: Berni
authors.forename: M A
authors.initials: MA
authors.lastname: Giambelluca
authors.forename: F
authors.initials: F
authors.lastname: Giannessi
mesh_terms: D000328:Adult; D016207:Cytokines / Q000276:immunology; D047150:Eosinophil Cationic Protein / Q000097:blood / Q000276:immunology; D004802:Eosinophilia / Q000097:blood / Q000276:immunology*; D004804:Eosinophils / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D018836:Inflammation Mediators / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D009298:Nasal Polyps / Q000097:blood / Q000276:immunology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D007073:Immunoglobulin E; D047150:Eosinophil Cationic Protein