title: Oxidative injury in rat fatty liver exposed to ischemia-reperfusion is modulated by nutritional status.
abstract: OBJECTIVE
  Oxidative stress contributes to ischemia-reperfusion injury in fatty livers. This study aimed to determine whether glycogen depletion influences this oxidative injury and whether the administration of glucose can be protective.
  
  
  METHODS
  Rats with choline deficiency-induced fatty liver underwent hepatic ischemia-reperfusion. Experimental groups: (1) fed rats; (2) 18 h fasted rats; (3) 18 h fasted rats supplemented with glucose prior to surgery. The thiobarbituric acid-reactive substances, protein carbonyls and total glutathione concentrations were measured in liver tissue and isolated mitochondria as parameters of oxidative stress before and after ischemia and during reperfusion. The mitochondrial F1-ATPase content and the serum alanine transaminase were also determined.
  
  
  RESULTS
  With respect to fed rats, fasted rats exhibited an increased oxidative injury in both liver tissue and isolated mitochondria throughout the experiment with the only exception of thiobarbituric acid-reactive substances, which were not affected by the nutritional status in liver tissue. Fasted rats showed a significantly lower F1-ATPase content and higher alanine transaminase levels. Glucose supplementation significantly reduced the fasting-associated exacerbation of oxidative stress and liver injury and the F1-ATPase exhaustion.
  
  
  CONCLUSIONS
  These data indicate that the pre-existing hepatic glycogen content modulates the oxidative damage in rat fatty livers exposed to ischemia-reperfusion injury and that the energetic substrate supplementation may represent a clinically feasible protective strategy.
authors.affiliation: Department of Internal Medicine, University of Bologna, Bologna, Italy; Center for Applied Biomedical Research (C.R.B.A.), University of Bologna, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Domenicali
authors.forename: G
authors.initials: G
authors.lastname: Vendemiale
authors.forename: G
authors.initials: G
authors.lastname: Serviddio
authors.forename: I
authors.initials: I
authors.lastname: Grattagliano
authors.forename: A M
authors.initials: AM
authors.lastname: Pertosa
authors.forename: B
authors.initials: B
authors.lastname: Nardo
authors.forename: A
authors.initials: A
authors.lastname: Principe
authors.forename: A
authors.initials: A
authors.lastname: Viola
authors.forename: F
authors.initials: F
authors.lastname: Trevisani
authors.forename: E
authors.initials: E
authors.lastname: Altomare
authors.forename: M
authors.initials: M
authors.lastname: Bernardi
authors.forename: P
authors.initials: P
authors.lastname: Caraceni
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D019587:Dietary Supplements; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009752:Nutritional Status; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D050050:Protein Carbonylation; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D000410:Alanine Transaminase; D006180:Proton-Translocating ATPases; D005978:Glutathione; D005947:Glucose