title: [An experimental study on injury of hippocampus neurons in rats by simulated push-pull maneuver of different degrees].
abstract: OBJECTIVE
  To explore the degree of injury and phase of push-pull effect induced by different degrees of push-pull maneuver on hippocampus neurons in rats.
  
  
  METHODS
  Injury of push-pull maneuver on hippocampus neurons in rats after different degrees of push-pull maneuver were determined by general evaluation of animal model, pathologic examination and electronic microscopy.
  
  
  RESULTS
  1) General evaluation of animal model: the apoplexy index and the pathological grading increased with the increase of intensity of the push-pull maneuver. 2) Routine HE: No abnormity was found in appearance and distribution of hippocampus neurons in the control rats and various groups of +/-2 Gz exposures, and 30 min groups of +/-6 Gz, +/-8 Gz exposures, but denatured necrosis of neurons were found in 6 h and 24 h groups of +/-6 Gz, +/-8 Gz exposures. 3) Examination of ultrastructure under electronic microscope: No significant change were found in hippocampus neurons of CA1 area in 30 min and 24 h after +/-2 Gz exposures as compared with the normal control group. But various degrees of denatured necrosis of neurons were found in some of the hippocampus neurons of CA1 area in 6 h after +/-2 Gz exposures, 6 h and 24 h after +/-6 Gz and +/-8 Gz exposures.
  
  
  CONCLUSIONS
  +/-6 Gz and +/-8 Gz exposures may cause injury of hippocampus tissues and it is most severe at 6 h after the +/-8 Gz exposures.
authors.affiliation: Jilin Military Medical College, The Fourth Military Medical University, Jilin, China.
authors.forename: Su-hong
authors.initials: SH
authors.lastname: Guo
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Wang
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Dou
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yun-yi
authors.initials: YY
authors.lastname: Li
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology; D004195:Disease Models, Animal; D006624:Hippocampus / Q000293:injuries / Q000473:pathology*; D018471:Hypergravity / Q000009:adverse effects*; D008854:Microscopy, Electron; D009336:Necrosis; D009474:Neurons / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000209:etiology / Q000473:pathology; D013997:Time Factors