title: Nonisothermal bioreactors in the treatment of vegetation waters from olive oil: laccase versus syringic acid as bioremediation model.
abstract: Laccase from Trametes versicolor was immobilized by diazotization on a nylon membrane grafted with glycidil methacrylate, using phenylenediamine as spacer and coupling agent. The behavior of these enzyme derivatives was studied under isothermal and nonisothermal conditions by using syringic acid as substrate, in view of the employment of these membranes in processes of detoxification of vegetation waters from olive oil mills. The pH and temperature dependence of catalytic activity under isothermal conditions has shown that these membranes can be usefully employed under extreme pH and temperatures. When employed under nonisothermal conditions, the membranes exhibited an increase of catalytic activity linearly proportional to the applied transmembrane temperature difference. Percentage activity increases ranging from 62% to 18% were found in the range of syringic acid concentration from 0.02 to 0.8 mM, when a difference of 1 degrees C was applied across the catalytic membrane. Because the percentage activity increase is strictly related to the reduction of the production times, the technology of nonisothermal bioreactors has been demonstrated to be an useful tool also in the treatment of vegetation waters from olive oil mills.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine and Surgery, Second University of Naples, Via S.M. di Costantinopoli 16, 80136 Naples, Italy.
authors.forename: Angelina
authors.initials: A
authors.lastname: Attanasio
authors.forename: Nadia
authors.initials: N
authors.lastname: Diano
authors.forename: Valentina
authors.initials: V
authors.lastname: Grano
authors.forename: Stefano
authors.initials: S
authors.lastname: Sicuranza
authors.forename: Sergio
authors.initials: S
authors.lastname: Rossi
authors.forename: Umberto
authors.initials: U
authors.lastname: Bencivenga
authors.forename: Luigi
authors.initials: L
authors.lastname: Fraconte
authors.forename: Silvana
authors.initials: S
authors.lastname: Di Martino
authors.forename: Paolo
authors.initials: P
authors.lastname: Canciglia
authors.forename: Damiano Gustavo
authors.initials: DG
authors.lastname: Mita
mesh_terms: D000383:Agriculture / Q000379:methods; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002384:Catalysis; D003198:Computer Simulation; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control; D042845:Laccase / Q000737:chemistry*; D008567:Membranes, Artificial; D008956:Models, Chemical; D000069463:Olive Oil; D010636:Phenols / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D013696:Temperature; D018508:Water Purification / Q000379:methods
chemical_list: D004800:Enzymes, Immobilized; D007220:Industrial Waste; D008567:Membranes, Artificial; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D005707:Gallic Acid; C001945:syringic acid; D042845:Laccase