The generation of immunotoxins using chimeric anti-CD22 antibodies containing mutations which alter their serum half-life.
Murine and chimeric RFB4 (anti-human CD22) monoclonal antibodies (MAbs) with mutations in their Fc portions were conjugated to recombinant ricin toxin A chain to generate immunotoxins. The resulting immunotoxins (ITs) constructed with chimeric RFB4 MAbs were designed to have longer or shorter half-lives but similar binding and cytotoxic properties. These ITs can now be evaluated in vivo for improved therapeutic indices. The characteristics of these ITs are the subject of this report.
Pop
Laurentiu M
LM
The Cancer Immunobiology Center, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., NB99.210 Dallas, TX 75390-8576, USA.
Liu
Xiaoyun
X
Ghetie
Victor
V
Vitetta
Ellen S
ES
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D015703:Antigens, CD / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006207:Half-Life; D006801:Humans; D000922:Immunotoxins / Q000493:pharmacokinetics* / Q000494:pharmacology; D037102:Lectins / Q000276:immunology*; D051379:Mice; D009154:Mutation; D012276:Ricin / Q000493:pharmacokinetics*; D051918:Sialic Acid Binding Ig-like Lectin 2; D020298:U937 Cells
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; C497868:Cd22 protein, mouse; D015815:Cell Adhesion Molecules; D000922:Immunotoxins; D037102:Lectins; D051918:Sialic Acid Binding Ig-like Lectin 2; D012276:Ricin