Presence of presenilin 1/2 affects the invasion and replication of Salmonella typhimurium.
In this study, we used four different cell lines, with or without presenilin-1 or -2, to investigate the hypothesis that the presence of presenilin, the most prominently mutated gene in Alzheimer's patients, affects the infection rate of host cells by Salmonella. The invasion and replication of Salmonella in presenilin 1/2-deficient cells were significantly lower than those in presenilin 1/2-expressing cells. Among several presenilin-interacting proteins, the expression of filamin-A in presenilin 1/2-deficient cells was significantly lower than in presenilin 1/2-expressing cells. However, Salmonella infection of filamin-A-deficient M2 cells did not significantly differ from infection of filamin-A-containing A7 cells, ruling out the possibility that filamin-A is a major protein inhibiting Salmonella invasion and replication. It is of interest to note that Hes-1 expression, a downstream target of Notch signaling pathway, was significantly decreased by Salmonella infection. Our results demonstrate that the presence of presenilins affects the invasion and replication processes of Salmonella.
Park
Jae-Hyo
JH
College of Pharmacy, Chonnam National University, Kwangju, Republic of Korea.
Kim
Hyun-ju
HJ
Choy
Hyon E
HE
Kim
Kwonseop
K
D000199:Actins / Q000378:metabolism; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D003285:Contractile Proteins / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology*; D064448:Filamins; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010587:Phagocytosis; D053764:Presenilin-1; D053766:Presenilin-2; D051880:Receptors, Notch; D012475:Salmonella / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism* / Q000472:pathogenicity*; D015398:Signal Transduction; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D000072056:Transcription Factor HES-1; D051176:beta Catenin
D000199:Actins; D051792:Basic Helix-Loop-Helix Proteins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D064448:Filamins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D008156:Luciferases; D008565:Membrane Proteins; D008840:Microfilament Proteins; D053764:Presenilin-1; D053766:Presenilin-2; D051880:Receptors, Notch; D015534:Trans-Activators; D000072056:Transcription Factor HES-1; D051176:beta Catenin; C495267:CTNNB1 protein, mouse; C508853:PSEN1 protein, human; C508861:PSEN2 protein, human; C078493:HES1 protein, human