Cyclic changes in messenger RNAs encoding inhibin/activin subunits in the ovary of the golden hamster (Mesocricetus auratus).
To elucidate changing patterns of inhibin/activin subunit mRNAs in the ovary of the golden hamster (Mesocricetus auratus) during the oestrous cycle, inhibin/activin subunit cDNAs of this species were cloned and ribonuclease protection assay and in situ hybridization were carried out. Inhibin alpha-subunit mRNA was localized in granulosa cells of primary, secondary, tertiary and atretic follicles throughout the 4-day oestrous cycle. It was also expressed in luteal cells on days 1 (oestrus), 2 (metoestrus) and 3 (dioestrus). betaA-subunit mRNA was localized in granulosa cells of large secondary (>200 microm) and tertiary follicles throughout the oestrous cycle. betaB-subunit mRNA was confined to granulosa cells of large secondary and tertiary follicles. Both alpha- and betaA-subunit mRNAs were also found in ovarian interstitial cells and theca interna cells of tertiary and atretic follicles in the evening of day 4 (pro-oestrus). A striking increase in betaA-subunit mRNA levels was also observed during the preovulatory period. The expression pattern of betaA-subunit mRNA during the preovulatory period is unique and not found in other species. An i.v. injection of anti-luteinizing hormone-releasing hormone (LHRH) serum before the LH surge abolished the expression of alpha- and betaA-subunit mRNAs in ovarian interstitial cells and theca interna cells. The treatment also abolished the preovulatory increase in betaA-subunit mRNA. Furthermore, administration of human chorionic gonadotrophin (hCG), which followed the injection of anti-LHRH serum, restored the expression patterns of alpha- and betaA-subunit mRNAs. The present study revealed that the golden hamster showed a unique expression pattern of betaA-subunit mRNA in response to the LH surge.
Arai
Koji Y
KY
Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Kishi
Hisashi
H
Onodera
Satoshi
S
Jin
Wanzhu
W
Watanabe
Gen
G
Suzuki
Akira K
AK
Takahashi
Shinji
S
Kamada
Toshihiko
T
Nishiyama
Toshio
T
Taya
Kazuyoshi
K
D028341:Activins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D006224:Cricetinae; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology; D006107:Granulosa Cells / Q000737:chemistry; D007106:Immune Sera / Q000494:pharmacology; D017403:In Situ Hybridization; D028322:Inhibin-beta Subunits / Q000235:genetics; D007265:Inhibins / Q000235:genetics*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D013799:Theca Cells / Q000737:chemistry
D006063:Chorionic Gonadotropin; D007106:Immune Sera; D012333:RNA, Messenger; C422362:inhibin A; C103008:inhibin beta A subunit; D028341:Activins; D007987:Gonadotropin-Releasing Hormone; D007265:Inhibins; D028322:Inhibin-beta Subunits