[Effects of aldose reductase on the expression of fibronectin and collagen IV in cultured rat renal mesangial cells].
OBJECTIVE
To study the effect of aldose reductase (AR) on expression of fibronectin and collagen IV in cultured rat renal mesangial cells (MsC).


METHODS
AR expression plasmid vector (pCDNA3-AR) was constructed by restriction endonuclease digestion and ligation procedures. Stable expression of AR in MsC was established by Lipofectin transfection. Western blot and immunofluorescence analyses were performed to verify the transfection efficiency. Expression of fibronectin and collagen IV proteins were analyzed using Western blot.


RESULTS
Expression of fibronectin and collagen IV in naive MsC treated with TGF-beta1 was upregulated in comparison to that of the untreated naive MsC (P < 0.01). MsC transfected with pCDNA3-AR showed a remarkable increase of expression of fibronectin and collagen IV (P < 0.01). Aldose reductase inhibitors (Sorbinil and Zopolrestat) significantly inhibited the expression of fibronectin and collagen IV in naive MsC (P < 0.05).


CONCLUSIONS
Overexpression or inhibition of AR activity significantly alters the expression of fibronectin and collagen IV proteins in cultured rat MsC, suggesting that AR plays a significant role in the pathogenesis of glomerulosclersis.
Jiang
Tao
T
Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Che
Qi
Q
Zhao
Zhong-hua
ZH
Zhang
Xiu-rong
XR
Zhang
Nong
N
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D052160:Benzothiazoles / Q000494:pharmacology; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D005822:Genetic Vectors; D048289:Imidazolidines / Q000494:pharmacology; D050527:Mesangial Cells / Q000378:metabolism*; D010793:Phthalazines / Q000494:pharmacology; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D052160:Benzothiazoles; D024141:Collagen Type IV; D005353:Fibronectins; D048289:Imidazolidines; D010793:Phthalazines; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; C067172:zopolrestat; D000449:Aldehyde Reductase; C026411:sorbinil