title: Antioxidant responses and oxidative stress after microcystin exposure in the hepatopancreas of an estuarine crab species.
abstract: Antioxidant responses and oxidative stress were evaluated in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after oral microcystin administration. Responses were evaluated through antioxidant enzyme activities (catalase-(CAT), superoxide dismutase, glutathione-S-transferase- (GST)). Nonproteic sulfhydril (NP-SH) groups, oxygen consumption, lipid peroxides (LPO), and oxidized proteins were also measured. Microcystin administration increased the oxygen consumption. GST activity and NP-SH concentration showed transient increases and CAT activity showed a peak and then a reduction. Oxidative damage was evidenced with regard to LPO content and suggested by the inhibition of CAT activity at the end of the experiment, indicating that the antioxidant response induced by the toxin was insufficient. A lowering in the number of hepatopancreatic B cells should be related to microcystin elimination.
authors.affiliation: Departamento de Ciências Fisiológicas, Fundação Universidade Federal do Rio Grande, R. Eng Alfredo Huch 475, 96201-900 Rio Grande, RS, Brazil.
authors.forename: G L L
authors.initials: GL
authors.lastname: Pinho
authors.forename: C Moura
authors.initials: CM
authors.lastname: da Rosa
authors.forename: F E
authors.initials: FE
authors.lastname: Maciel
authors.forename: A
authors.initials: A
authors.lastname: Bianchini
authors.forename: J S
authors.initials: JS
authors.lastname: Yunes
authors.forename: L A O
authors.initials: LA
authors.lastname: Proença
authors.forename: J M
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000502:physiology*; D003386:Brachyura / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D043143:Hepatopancreas / Q000502:physiology*; D052998:Microcystins; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010456:Peptides, Cyclic / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D005982:Glutathione Transferase