title: Analysis of SNP profiles in patients with major depressive disorder.
abstract: The present study focused on 91 single-nucleotide polymorphisms (SNPs) in 21 candidate genes to find associations with major depressive disorder (MDD). In total, 160 healthy controls and 177 patients with MDD were studied. We applied arrayed primer extension (APEX) based genotyping technology followed by association and haplotype analysis. SNPs in CCKAR, DRD1, DRD2, and HTR2C genes showed nominally significant associations with MDD. None of these associations remained significant after adjustment for multiple testing. Haplotype analysis revealed CCKAR haplotypes to be associated with MDD (global p=0.004). More precisely, we found the GAGT haplotype to be associated with increased risk for MDD (OR 7.42, 95% CI 2.13-25.85, p=0.002). This haplotype effect remained significant after Bonferroni correction (p=0.04 after Bonferroni's adjustment). Altogether we were able to find some nominal associations, but due to small sample size these results should be taken as exploratory. However, the effect of GAGT haplotype on the CCKAR gene may be considered as increasing the risk for MDD.
authors.affiliation: Department of Physiology, University of Tartu, Tartu, EstoniaVisgenyx Ltd, Tartu, Estonia. Sulev.Koks@ut.ee
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
authors.forename: Tiit
authors.initials: T
authors.lastname: Nikopensius
authors.forename: Kati
authors.initials: K
authors.lastname: Koido
authors.forename: Eduard
authors.initials: E
authors.lastname: Maron
authors.forename: Signe
authors.initials: S
authors.lastname: Altmäe
authors.forename: Evelin
authors.initials: E
authors.lastname: Heinaste
authors.forename: Kristel
authors.initials: K
authors.lastname: Vabrit
authors.forename: Veronika
authors.initials: V
authors.lastname: Tammekivi
authors.forename: Pille
authors.initials: P
authors.lastname: Hallast
authors.forename: Ants
authors.initials: A
authors.lastname: Kurg
authors.forename: Jakov
authors.initials: J
authors.lastname: Shlik
authors.forename: Veiko
authors.initials: V
authors.lastname: Vasar
authors.forename: Andres
authors.initials: A
authors.lastname: Metspalu
authors.forename: Eero
authors.initials: E
authors.lastname: Vasar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D016001:Confidence Intervals; D003865:Major Depressive Disorder / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D043886:Receptor, Cholecystokinin A / Q000235:genetics
chemical_list: D043886:Receptor, Cholecystokinin A