title: Protein crystal perfection and its application.
abstract: Knowledge of protein crystal perfection in theory and practice is reviewed. X-ray methods of assessing perfection such as mosaicity, topography and reciprocal-space mapping are described. X-ray diffraction physics applications of protein crystals such as in Laue geometry, the large-angle oscillation technique and forming polychromatic profiles across diffraction spots are covered. Cryo- and room-temperature cases are discussed including in X-ray and neutron protein crystallography. Experience of freezing very large crystals, which are commonly used today in neutron protein crystallography, is highlighted.
authors.affiliation: Department of Chemistry, University of Manchester, M13 9PL, England.
authors.forename: John R
authors.initials: JR
authors.lastname: Helliwell
mesh_terms: D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins