title: [Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2].
abstract: OBJECTIVE
  To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.
  
  
  METHODS
  Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.
  
  
  RESULTS
  PC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.
  
  
  CONCLUSIONS
  RNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.
authors.affiliation: Institute of Biotechnology, Military Academy of Medical Science, Beijing 100850, China.
authors.forename: Li-quan
authors.initials: LQ
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Xue-song
authors.initials: XS
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Rui-xia
authors.initials: RX
authors.lastname: Liang
authors.forename: Bao-fa
authors.initials: BF
authors.lastname: Hong
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011755:Pyrophosphatases / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases