Benzimidazole derivatives as potent inhibitors of milk xanthine oxidase.
The inhibitory properties of a series of benzimidazole derivatives (which are different in the substituents at positions C2, C5, C6) in the reaction with milk xanthine oxidase have been investigated. The inhibition of xanthine hydroxylation process was monitored at different pH solutions using UV absorption spectroscopy. The efficient inhibition was observed in the case, if the benzene ring of the benzimidazoles was substituted at C5 and C6 positions with Cl or NO2 but not with methyl groups. The inhibition potency increased, when 5,6-dichloro or 5,6-dinitrobenzimidazoles were substituted at C2 position with fluoroalkyl groups.
Rudnicka
Renata
R
Department of Biophysics, University of Warsaw, 93 Zwirki & Wigury Str., 02-089 Warsaw.
Bojarska
Elzbieta
E
Kazimierczuk
Zygmunt
Z
D000818:Animals; D001562:Benzimidazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology*; D008892:Milk / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
D001562:Benzimidazoles; D004791:Enzyme Inhibitors; D014969:Xanthine Oxidase