[The role of cyclic AMP in repair of hemisection of spinal cord in rats models].
OBJECTIVE
To study the role of cAMP in repair of hemisection of spinal cord in rats models.


METHODS
Rats models of spinal cord hemisection were made and cAMP were injected once in the motor cortex or continuously input in the lesion area or in the subarachnoid cistern for 3 d. NFs, GFAP, CSTs and spinal axons in the lesion areas were observed by immunohistochemistry and hind limb movements were evaluated in BBB scales.


RESULTS
Many regenerated axons were presented in the lesion areas in cAMP groups though no continuous long regenerated axons traversed the lesion area when cAMP was input in the motor cortex or in the local lesion area. In control group, no regenerated axon were presented in the lesion areas. When cAMP was input in the subarachnoid cistern, only few-labelled CST axon survived and presented in the lesion area comparing no labelled CST axon presented in the lesion area. More NFs and less GFAP were distributed and extended in the lesion area in the cAMP groups. All the rats restored to normally walk 4-5 weeks after operations and no significance existed between cAMP groups and control groups comparing the BBB scales of hind limb movements.


CONCLUSIONS
cAMP injected in the brain cortex or continuously input in the lesion area can induce the axonal regeneration.
Chen
Xiang-rong
XR
Department of Orthopaedical Surgery, Nan Fang Hospital, the First Military Medical University, Guangzhou 510515, China.
You
Si-wei
SW
Jin
Da-di
DD
D000287:Administration, Topical; D000818:Animals; D000242:Cyclic AMP / Q000008:administration & dosage* / Q000502:physiology; D006614:Hindlimb / Q000503:physiopathology; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology
D000242:Cyclic AMP