Isolation and expression of a novel molecular class D beta-lactamase, OXA-61, from Campylobacter jejuni.
A novel beta-lactamase gene, blaOXA-61, from Campylobacter jejuni GC015 was cloned and its nucleotide sequence determined. blaOXA-61 encodes a protein of 257 amino acids in which the active-site STFK tetrad and conserved class D beta-lactamase motifs YGN and KTG were identified. A conserved sequence upstream of blaOXA-61 is required for expression in Campylobacter.
Alfredson
David A
DA
Microbial Glycobiology, Institute for Glycomics, Griffith University and Microbiology Department, Queensland Health Pathology Services, Gold Coast Hospital, 108 Nerang St., Southport, Queensland, Australia 4215. David_Alfredson@health.qld.gov.au
Korolik
Victoria
V
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases