title: Influence of alginate on type II collagen fibrillogenesis.
abstract: Collagen II is the majority of extracellular matrix components in articular cartilage, which with the major functions of preventing expansion of the tissue and distributing the load of body weight. To obtain man-made ECM, the reconstitution of collagen could be conducted in the presence of negatively charged polysaccharide, such as alginate. Alginate is an anionic polysaccharide capable of eversible gelated in calcium ion solution to prepare different shapes of biomaterials. Its well-known biocompatibility makes it an ideal material in biomedical applications. Thus, the aim of this study was to evaluate the effects of alginate on the fibrillogenesis of type II collagen. The preliminary results revealed that inclusion of alginate into soluble type II collagen solution could inhibit the development of turbidity of collagen solution, and the apparent rate constants in lag and growth phases decreased during collagen formation period, both rate constants decreased to about one-third of the original constants, respectively. From TEM observations, the collagen fibrils were significantly thicker in 0.05% and 0.1% alginate as compared with pure collagen solution. Furthermore, the D-periods of collagen fibers kept unchanged significantly under all reconstituted conditions, which meant the packing of collagen monomer was probably not affected by adding these amounts of alginate.
authors.affiliation: Department of Biomedical Engineering, I-SHOU University, Kaohsiung County, Taiwan.
authors.forename: Shyh Ming
authors.initials: SM
authors.lastname: Kuo
authors.forename: Yng Jiin
authors.initials: YJ
authors.lastname: Wang
authors.forename: Chin Lung
authors.initials: CL
authors.lastname: Weng
authors.forename: Huai En
authors.initials: HE
authors.lastname: Lu
authors.forename: Shwu Jen
authors.initials: SJ
authors.lastname: Chang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D024043:Collagen Type II / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D019281:Dimerization; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D024043:Collagen Type II; D006603:Hexuronic Acids; D046912:Multiprotein Complexes; D020723:Glucuronic Acid