title: Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha).
abstract: Four cytosolic glutathione S-transferase (GST) classes were isolated and characterized from juvenile winter run Chinook salmon (Oncorhynchus tshawytscha) liver. Two techniques were used: (1) gel electrophoresis/immunoblotting against a polyclonal striped bass GST antibody and (2) high-pressure liquid chromatography (HPLC). Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as pi, theta, mu and alpha, by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid (ETHA) were determined to be 0.3+/-0.05 U/mg cytosolic protein and 0.06+/-0.02 U/mg cytosolic protein, respectively.
authors.affiliation: Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.
authors.forename: Rachel T
authors.initials: RT
authors.lastname: Donham
authors.forename: Dexter
authors.initials: D
authors.lastname: Morin
authors.forename: William T
authors.initials: WT
authors.lastname: Jewell
authors.forename: M W
authors.initials: MW
authors.lastname: Lamé
authors.forename: H J
authors.initials: HJ
authors.lastname: Segall
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Tjeerdema
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D019295:Computational Biology; D003600:Cytosol / Q000201:enzymology*; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004976:Ethacrynic Acid / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification*; D015151:Immunoblotting; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012474:Salmon / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase; D004976:Ethacrynic Acid