Molecular evolution of flavonoid dioxygenases in the family Apiaceae.
Plant species of the family Apiaceae are known to accumulate flavonoids mainly in the form of flavones and flavonols. Three 2-oxoglutarate-dependent dioxygenases, flavone synthase or flavanone 3 beta-hydroxylase and flavonol synthase are involved in the biosynthesis of these secondary metabolites. The corresponding genes were cloned recently from parsley (Petroselinum crispum) leaves. Flavone synthase I appears to be confined to the Apiaceae, and the unique occurrence as well as its high sequence similarity to flavanone 3beta-hydroxylase laid the basis for evolutionary studies. In order to examine the relationship of these two enzymes throughout the Apiaceae, RT-PCR based cloning and functional identification of flavone synthases I or flavanone 3beta-hydroxylases were accomplished from Ammi majus, Anethum graveolens, Apium graveolens, Pimpinella anisum, Conium maculatum and Daucus carota, yielding three additional synthase and three additional hydroxylase cDNAs. Molecular and phylogenetic analyses of these sequences were compatible with the phylogeny based on morphological characteristics and suggested that flavone synthase I most likely resulted from gene duplication of flavanone 3beta-hydroxylase, and functional diversification at some point during the development of the apiaceae subfamilies. Furthermore, the genomic sequences from Petroselinum crispum and Daucus carota revealed two introns in each of the synthases and a lack of introns in the hydroxylases. These results might be explained by intron losses from the hydroxylases occurring at a later stage of evolution.
Gebhardt
Yvonne
Y
Philipps Universität Marburg, Institut für Pharmazeutische Biologie, Deutschhausstr. 17A, D-35037 Marburg/Lahn, Germany.
Witte
Simone
S
Forkmann
Gert
G
Lukacin
Richard
R
Matern
Ulrich
U
Martens
Stefan
S
D000595:Amino Acid Sequence; D019661:Apiaceae / Q000201:enzymology* / Q000235:genetics*; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism*; D019143:Evolution, Molecular; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D008956:Models, Chemical; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism
D005419:Flavonoids; D010940:Plant Proteins; D049308:Dioxygenases