title: Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo.
abstract: The heat shock cognate 70-4 protein gene promoter (HSC70-4p) from Bombyx mori (BmHSC70-4p) is an ideal candidate for the transgenic silkworm due to its high transcriptional activity, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. Using luciferase as a reporter gene and transient expression system in vivo and in vitro, we found that BmNPVhr3 can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Moreover, the transcriptional activity of the combination of BmHSC70-4p and BmNPVhr3 changed with developmental stages and hormone titers. These results suggest that the combination of BmHSC70-4 promoter and BmNPVhr3 enhancer is more effective candidate for the transgene or stable cell expression system in Bombyx mori.
authors.affiliation: Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang.
authors.forename: Shunming
authors.initials: S
authors.lastname: Tang
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yongzhu
authors.initials: Y
authors.lastname: Yi
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yiren
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D007605:Juvenile Hormones / Q000502:physiology; D007814:Larva; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D006360:Heat-Shock Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones