Survival adjustment of mature dendritic cells by IL-15.
The survival of CD8+/CD44(hi) memory phenotype T cells depends on an IL-15 activity on nonlymphoid cells. Here, we report that IL-15 and its receptor were induced on dendritic cells (DCs) by a combination of IFN-gamma and NF-kappaB relA inducers. IL-15 conferred in an autocrine loop resistance to apoptosis that accompanied the maturation process in DCs in vitro. As an apparent result in vivo, mice deficient in IL-15 or its receptor harbor few DCs. Injecting DCs into IL-15-/- mice was associated with the appearance of CD8+/CD44(hi) T cells that depended on IL-15 expression but also correlated with the longevity of the DCs. These findings support the hypothesis that DCs mediate the effect of IL-15 on CD8+/CD44(hi) memory phenotype T cells.
Dubois
Sigrid P
SP
Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Waldmann
Thomas A
TA
Müller
Jürgen R
JR
D000818:Animals; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002470:Cell Survival / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D019254:Genes, bcl-2 / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D018960:Hyaluronan Receptors / Q000378:metabolism; D047468:Immunoprecipitation; D007371:Interferon-gamma; D019409:Interleukin-15 / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B; D018123:Receptors, Interleukin / Q000378:metabolism*; D012190:Retroviridae
D018960:Hyaluronan Receptors; D019409:Interleukin-15; D016328:NF-kappa B; D018123:Receptors, Interleukin; D049452:Green Fluorescent Proteins; D007371:Interferon-gamma