title: Vascular ENaC proteins are required for renal myogenic constriction.
abstract: The myogenic response is an essential component of renal blood flow autoregulation and is the inherent ability of vascular smooth muscle cells (VSMCs) to contract in response to increases in intraluminal pressure. Although mechanosensitive ion channels are thought to initiate VSMC stretch-induced contraction, their molecular identity is unknown. Recent reports suggest degenerin/epithelial Na(+) channels (DEG/ENaC) may form mechanotransducers in sensory neurons and VSMCs; however, the role of DEG/ENaC proteins in myogenic constriction of mouse renal arteries has not been established. To test the hypothesis that DEG/ENaC proteins are required for myogenic constriction in renal vessels, we first determined expression of ENaC transcripts and proteins in mouse renal VSMCs. Then, we determined pressure- and agonist-induced constriction and changes in vascular smooth muscle cytosolic Ca(2+) and Na(+) in isolated mouse renal interlobar arteries following DEG/ENaC inhibition with amiloride and benzamil. We detect alpha-, beta-, and gammaENaC transcript and protein expression in cultured mouse renal VSMC. In contrast, we detect only beta- and gamma- but not alphaENaC protein in freshly dispersed mrVMSC. Selective DEG/ENaC inhibition, with low doses of amiloride and benzamil, abolishes pressure-induced constriction and increases in cytosolic Ca(2+) and Na(+) without diminishing agonist-induced responses in isolated mouse interlobar arteries. Our findings indicate that DEG/ENaC proteins are required for myogenic constriction in mouse interlobar arteries and are consistent with our hypothesis that DEG/ENaC proteins may be components of mechanosensitive ion channel complexes required for myogenic vasoconstriction.
authors.affiliation: Dept. of Physiology and Biophysics, Univ. of Mississippi Medical Center, 2500 North State St., Jackson, MS 39216, USA.
authors.forename: Nikki L
authors.initials: NL
authors.lastname: Jernigan
authors.forename: Heather A
authors.initials: HA
authors.lastname: Drummond
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003600:Cytosol / Q000378:metabolism; D004232:Diuretics / Q000494:pharmacology; D053503:Epithelial Sodium Channels; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D051379:Mice; D009119:Muscle Contraction / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D012077:Renal Artery / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D004232:Diuretics; D053503:Epithelial Sodium Channels; D015222:Sodium Channels; D014662:Vasoconstrictor Agents; C013407:benzamil; D011189:Potassium Chloride; D000584:Amiloride; D012964:Sodium; D002118:Calcium