Flow injection analysis of riboflavin with chemiluminescence detection using a N-halo compounds-luminol system.
A chemiluminescent method for the determination of riboflavin is described. The method is based on the chemiluminescence (CL) generated during the oxidation of luminol by N-bromosuccinimide (NBS) and N-chlorosuccinimide (NCS) in alkaline medium. It was found that riboflavin could greatly enhance this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of riboflavin is proposed in this paper. The detection limits were 7.5 ng/mL and 3.5 ng/mL of riboflavin for the NBS- and NCS-luminol CL systems, respectively. The relative CL intensity was linear, with riboflavin concentration in the range 19-600 ng/mL and 600-2000 ng/mL for the NBS-luminol CL system, and 12-200 ng/mL and 200-2000 ng/mL for the NCS-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official method and demonstrated good accuracy and precision.
Safavi
A
A
Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran. safavi@chem.susc.ac.ir
Karimi
M A
MA
Nezhad
M R Hormozi
MR
D001974:Bromosuccinimide; D002138:Calibration; D004867:Equipment Design; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods; D007202:Indicators and Reagents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008165:Luminol / Q000737:chemistry*; D004364:Pharmaceutical Preparations; D012256:Riboflavin / Q000032:analysis* / Q000592:standards; D013388:Succinimides
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D013388:Succinimides; C015752:N-chlorosuccinimide; D008165:Luminol; D001974:Bromosuccinimide; D012256:Riboflavin