title: Deletion of the datA site does not affect once-per-cell-cycle timing but induces rifampin-resistant replication.
abstract: In Escherichia coli, three mechanisms have been proposed to maintain proper regulation of replication so that initiation occurs once, and only once, per cell cycle. First, newly formed origins are inactivated by sequestration; second, the initiator, DnaA, is inactivated by the Hda protein at active replication forks; and third, the level of free DnaA protein is reduced by replication of the datA site. The datA site titrates unusually large amounts of DnaA and it has been reported that reinitiation, and thus asynchrony of replication, occurs in cells lacking this site. Here, we show that reinitiation in deltadatA cells does not occur during exponential growth and that an apparent asynchrony phenotype results from the occurrence of rifampin-resistant initiations. This shows that the datA site is not required to prevent reinitiation and limit initiation of replication to once per generation. The datA site may, however, play a role in timing of initiation relative to cell growth. Inactivation of active ATP-DnaA by the Hda protein and the sliding clamp of the polymerase was found to be required to prevent reinitiation and asynchrony of replication.
authors.affiliation: Department of Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway.
authors.lastname: Morigen
authors.forename: Felipe
authors.initials: F
authors.lastname: Molina
authors.forename: Kirsten
authors.initials: K
authors.lastname: Skarstad
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology; D004269:DNA, Bacterial / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D017353:Gene Deletion; D016296:Mutagenesis; D010641:Phenotype; D018741:Replication Origin; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D029968:Escherichia coli Proteins; D000251:Adenosine Triphosphatases; C435211:Hda protein, E coli; D012293:Rifampin