[The immunohistochemical observations of NOS in the nasal mucosa of allergic rhinitis animal model of guinea pigs].
OBJECTIVE
To study the immunoreactivity and distribution of both iNOS and eNOS isoforms, and observe the effect of desloratadine on them.


METHODS
The guinea pigs were sensitized and challenged followed by harvest of their nasal tissue for immunohistochemical staining. The slides images were semiquantitatively analyzed and compared with the desloratadine treated group and negative control group.


RESULTS
Both iNOS and eNOS were positively stained in each group. The immunoreactivity of iNOS had no significant difference between groups (P > 0.05), but eNOS had stronger immunoreactivity in the model group and the desloratadine treated group when compared with the negative control group (P < 0.01 and P < 0.05). In addition to the distribution area of iNOS, eNOS was also positive stained in the goblet cells. Desloratadine had no influence on iNOS and eNOS immunoreactivity.


CONCLUSIONS
eNOS might play a more important role than iNOS in regulating the NO level of the nasal tissue of the guinea pigs suffered from allergic rhinitis, and desloratadine had no involvement in regulating the expression of iNOS and eNOS.
Zhao
Yu
Y
Department of Otolaryngology, Prince of Wales Hospital, Chinese University of Hongkong, Shatin, NT, Hongkong SAR, China. zhaoyu@surgery.cuhk.edu.hk
Van Hasselt
C A
CA
Woo
Kangsang
K
Wong
Yeukoi
Y
Leung
Pingcheung
P
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis; D017336:Loratadine / Q000494:pharmacology; D009297:Nasal Mucosa / Q000201:enzymology* / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis*; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology
D018926:Anti-Allergic Agents; D007527:Isoenzymes; D017336:Loratadine; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III