title: Continuous beer fermentation using immobilized yeast cell bioreactor systems.
abstract: Traditional beer fermentation and maturation processes use open fermentation and lager tanks. Although these vessels had previously been considered indispensable, during the past decades they were in many breweries replaced by large production units (cylindroconical tanks). These have proved to be successful, both providing operating advantages and ensuring the quality of the final beer. Another promising contemporary technology, namely, continuous beer fermentation using immobilized brewing yeast, by contrast, has found only a limited number of industrial applications. Continuous fermentation systems based on immobilized cell technology, albeit initially successful, were condemned to failure for several reasons. These include engineering problems (excess biomass and problems with CO(2) removal, optimization of operating conditions, clogging and channeling of the reactor), unbalanced beer flavor (altered cell physiology, cell aging), and unrealized cost advantages (carrier price, complex and unstable operation). However, recent development in reactor design and understanding of immobilized cell physiology, together with application of novel carrier materials, could provide a new stimulus to both research and application of this promising technology.
authors.affiliation: Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Tomás
authors.initials: T
authors.lastname: Brányik
authors.forename: António A
authors.initials: AA
authors.lastname: Vicente
authors.forename: Pavel
authors.initials: P
authors.lastname: Dostálek
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
mesh_terms: D001515:Beer / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods* / Q000639:trends; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D018914:Cells, Immobilized / Q000502:physiology; D004867:Equipment Design; D005285:Fermentation / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*