title: Enhanced production of manganese peroxidase from immobilized Phanerochaete chrysosporium due to the increased autolysis of chlamydospore-like cells.
abstract: The autolysis of chlamydospore-like cells in Phanerochaete chrysosporium immobilized in polyurethane foam correlated with the production of manganese peroxidase (MnP). The maximum specific activity of MnP was 1055 U g dry mycelium(-1) in the immobilized culture, compared with 260 U g dry mycelium(-1) in the submerged culture. Scattered mycelial pellets were formed in the immobilized culture in which almost all of the chlamydospore-like cells were subject to autolysis. However, highly crowded pellets were formed in the free culture, in which only the chlamydospore-like cells in the exterior were subject to autolysis. We propose that the enhanced production of MnP in immobilized cultures of P. chrysosporium is due to increased autolysis of the chlamydospore-like cells.
authors.affiliation: Department of Chemical Engineering, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Eunyong
authors.initials: E
authors.lastname: Chung
authors.forename: Junse
authors.initials: J
authors.lastname: Oh
authors.forename: Sangpill
authors.initials: S
authors.lastname: Hwang
authors.forename: Ik-Sung
authors.initials: IS
authors.lastname: Ahn
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
mesh_terms: D018533:Biomass; D018914:Cells, Immobilized; D005285:Fermentation; D008858:Microscopy, Phase-Contrast; D025282:Mycelium / Q000166:cytology; D010544:Peroxidases / Q000096:biosynthesis* / Q000302:isolation & purification*; D020075:Phanerochaete / Q000378:metabolism*; D011140:Polyurethanes; D013172:Spores, Fungal / Q000166:cytology
chemical_list: D011140:Polyurethanes; D010544:Peroxidases; C051129:manganese peroxidase