Photoperiod regulates clock gene rhythms in the ovine liver.
To investigate the photoperiodic entrainment of peripheral rhythms in ruminants, we studied the expression of clock genes in the liver in the highly seasonal Soay sheep. Animals were kept under long (LD 16:8) or short photoperiod (LD 8:16). Daily rhythms in locomotor activity were recorded, and blood concentrations of melatonin and cortisol were measured by RIA. Per2, Bmal1, and Cry1 gene expression was determined by Northern blot analyses using ovine RNA probes in liver collected every 4h for 24h. Liver Per2 and Bmal1, but not Cry1, expression was rhythmic in all treatments. Under long days, peak Per2 expression occurred at end of the night with a similar timing to Bmal1, whereas, under short days the Per2 maximum was in the early night with an inverse pattern to Bmal1. There was a photoperiodxtime interaction for only Per2 (P < 0.001). The 24-h pattern in plasma cortisol matched the observed phasing of Per2 expression, suggesting that it may act as an endocrine entraining factor. The clock gene rhythms in the peripheral tissues were different in timing compared with the ovine suprachiasmatic nucleus (SCN, central pacemaker) and pars tuberalis (melatonin target tissue), and the hepatic rhythms were of lower amplitude compared with photoperiodic rodents. Thus, there are likely to be important species differences in the way the central and peripheral clockwork encodes external photoperiod.
Andersson
Håkan
H
Medical Research Council, Human Reproductive Sciences Unit, Centre for Reproductive Biology, Edinburgh, Scotland, UK.
Johnston
Jonathan D
JD
Messager
Sophie
S
Hazlerigg
David
D
Lincoln
Gerald
G
D056930:ARNTL Transcription Factors; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D015152:Blotting, Northern / Q000662:veterinary*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D056931:Cryptochromes; D005260:Female; D005420:Flavoproteins / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood; D008099:Liver / Q000502:physiology*; D008550:Melatonin / Q000097:blood; D009043:Motor Activity / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D017440:Photoperiod; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012756:Sheep / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D056930:ARNTL Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D056931:Cryptochromes; D005420:Flavoproteins; D006854:Hydrocortisone; D008550:Melatonin; D009687:Nuclear Proteins; D012313:RNA; D014157:Transcription Factors; C538960:BMAL1 protein, human; C538963:CRY1 protein, human