title: Nonactin biosynthesis: the product of the resistance gene degrades nonactin stereospecifically to form homochiral nonactate dimers.
abstract: Heterologous expression of the tetranactin resistance gene nonR in E. coli gave a his6-tagged protein that catalyzed the stereoselective hydrolysis of the chiral macrotetrolide antibiotic nonactin into equivalent, chiral, inactive nonactate dimer species. No trimeric nonactate was produced in the reaction.
authors.affiliation: Department of Chemistry, The University of Montana, Missoula, Montana 59812-1656, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Cox
authors.forename: Nigel D
authors.initials: ND
authors.lastname: Priestley
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D018942:Macrolides / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018942:Macrolides; C089115:ORF6 protein, Bacteria; C050476:nonactin