Different response of the knockout mice lacking b-series gangliosides against botulinum and tetanus toxins.
We assessed the response in knockout mice lacking the b-series (G(D2), G(D1b), G(T1b) and G(Q1b)) gangliosides against Clostridium botulinum (types A, B and E) and tetani toxins. We found that botulinum toxins were fully toxic, while tetanus toxin was much less toxic in the knockout mice. Combining the present results with our previous finding that tetanus toxin and botulinum types A and B toxins showed essentially no toxic activity in the knockout mice lacking both the a-series and b-series gangliosides (complex gangliosides), we concluded that the b-series gangliosides is the major essential substance for tetanus toxin, while b-series gangliosides may be not the essential substance for botulinum toxins, at the initial step during the intoxication process in mouse.
Kitamura
Masaru
M
Division of Biomedical Food Research, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan. kmasaru@ninus.ocn.ne.jp
Igimi
Shizunobu
S
Furukawa
Keiko
K
Furukawa
Koichi
K
D000818:Animals; D001905:Botulinum Toxins / Q000378:metabolism / Q000633:toxicity*; D003014:Clostridium botulinum; D004305:Dose-Response Relationship, Drug; D005260:Female; D005732:Gangliosides / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D006720:Homozygote; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D015996:Survival Rate; D013744:Tetanus Toxin / Q000378:metabolism / Q000633:toxicity*
D005732:Gangliosides; D013744:Tetanus Toxin; D001905:Botulinum Toxins