title: Impact of donor gender on male rat recipients of small-for-size liver grafts.
abstract: The aim of this study was to assess the impact of donor gender on small-for-size (SFS) liver transplantation in male recipients using a rat model. Adult female or male Lewis rats were used as donors and male Lewis rats as recipients. Size-matched (SM) and SFS liver grafts from either male or female donors were transplanted into male recipients. Animals receiving SFS grafts were sacrificed at postoperative week 1, week 4, and week 12, respectively (n = 6-8 per group), those receiving SM grafts after 3 months. The cumulative survival rate (SVR) in the female-to-male (F-M) SFS group was significantly lower (62%; 13 of 21) compared with the male-to-male (M-M) group (90%; 18 of 20) (P < 0.05). Spontaneous death occurred in the F-M SFS combination either in the early postoperative period (<3 weeks) in animals with confluent hepatic necrosis or in the late postoperative period (>8 weeks) in animals with biliary obstruction. In contrast, no death was observed in the early posttransplantation period after M-M liver transplantation. The relative graft size in the SM F-M group was significantly higher (graft-to-recipient weight ratio [GRWR] 2.40% +/- 0.8%) than in the SFS M-M group (GRWR 1.35% +/- 0.2%; P < 0.001). Regardless of graft size, the outcome was worse in terms of SVR as well as regarding the incidence and severity of biliary complications in F-M compared with M-M liver transplantation. In conclusion, male recipients of female livers had a less favorable outcome irrespective of graft size. Confluent hepatic necrosis as well as biliary obstruction were perceived as consequence of a severe perfusion problem in F-M liver transplantation, which was possibly related to an enhancement of ischemia-reperfusion (I/R) injury by the lack of estrogen in male recipients of female grafts.
authors.affiliation: Department of General Surgery and Transplantation Surgery, University Hospital Duisburg-Essen, Essen, Germany.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Gu
authors.forename: Olaf
authors.initials: O
authors.lastname: Dirsch
authors.forename: Uta
authors.initials: U
authors.lastname: Dahmen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Haidong
authors.initials: H
authors.lastname: Chi
authors.forename: Christoph Erich
authors.initials: CE
authors.lastname: Broelsch
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D008099:Liver / Q000033:anatomy & histology*; D016031:Liver Transplantation / Q000009:adverse effects / Q000379:methods*; D008297:Male; D009929:Organ Size; D011183:Postoperative Complications; D011336:Probability; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D012737:Sex Factors; D015996:Survival Rate; D014019:Tissue Donors