Association between beta-adrenergic receptor polymorphisms and their G-protein-coupled receptors with body mass index and obesity in women: a report from the NHLBI-sponsored WISE study.
OBJECTIVE
The beta-adrenergic receptor (betaAR) genes are candidate genes for obesity because of their roles in energy homeostasis and promotion of lipolysis in human adipose tissue. Objective is to determine the association between obesity and polymorphisms in genes of the beta(1)AR (ADRB1), beta(2)AR (ADRB2), beta(3)AR (ADRB3), Gs protein alpha (GNAS1), to which all three beta-receptors couple and the G protein beta3 subunit (GNB3), to which beta(3)ARs couple.


METHODS
A case-control genetic association study.


METHODS
A total of 643 black or white women enrolled in Women's Ischemia Syndrome Evaluation (WISE) study.


METHODS
Genotypes were determined by PCR with single primer extension. Associations between genotype and body mass index (BMI), waist-to-hip ratio (WHR), waist circumference, and obesity were made.


RESULTS
Polymorphisms in the three betaAR genes, GNAS1, and GNB3 were not associated with BMI, WHR, waist circumference, or obesity. Linear and logistic regression analyses found no contribution of either genotype or haplotype with anthropometric measurements or obesity.


CONCLUSIONS
Our study suggests that among American women with suspected coronary heart disease, polymorphisms in the betaARs and their G-protein-coupled receptors do not contribute to increased BMI, WHR, waist circumference, or obesity. Given that 50% of all women die from coronary heart disease, and a higher percentage have heart disease during their lifetime, our results are likely generalizable to many American women.
Terra
S G
SG
Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL 32610, USA.
McGorray
S P
SP
Wu
R
R
McNamara
D M
DM
Cavallari
L H
LH
Walker
J R
JR
Wallace
M R
MR
Johnson
B D
BD
Bairey Merz
C N
CN
Sopko
G
G
Pepine
C J
CJ
Johnson
J A
JA
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044383:Black People; D015992:Body Mass Index; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D016015:Logistic Models; D008875:Middle Aged; D009765:Obesity / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D044465:White People
D011943:Receptors, Adrenergic, beta; D043562:Receptors, G-Protein-Coupled