Characterization of medroxyprogesterone and DMBA-induced multilineage mammary tumors by gene expression profiling.
Mouse mammary tumors arising during medroxyprogesterone-DMBA-mediated mammary carcinogenesis comprised three distinct phenotypes: adenocarcinoma, squamous cell carcinoma, and myoepithelial carcinoma. The molecular signature for each of the three tumor subsets was characterized by gene microarray analysis, and three distinct sets of gene expression profiles were obtained that were corroborated in part by quantitative RT-PCR and immunohistochemistry. These results suggest that this carcinogenesis and gene expression model will be useful for rapidly assessing the histopathological differences arising in mammary carcinogenesis and the effects of tumor promoting or chemoprevention agents.
Yin
Yuzhi
Y
Department of Oncology, and Lombardi Comprehensive Cancer Center, Georgetown University, Washington, District of Columbia, USA.
Bai
Renkui
R
Russell
Robert G
RG
Beildeck
Marcy E
ME
Xie
Zhihui
Z
Kopelovich
Levy
L
Glazer
Robert I
RI
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D007150:Immunohistochemistry; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008525:Medroxyprogesterone / Q000494:pharmacology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D015127:9,10-Dimethyl-1,2-benzanthracene; D008525:Medroxyprogesterone