[Analysis of gene expression information in the fat and muscle tissues of pig].
An in silico study was developed to detect the gene expression profile of pig fat and muscle tissues by using the porcine EST resources and human gene sequences,and thus provided candidate information for basic genetic analysis in meat quality improvement of pig. In this study,a BLAST search was performed to identify homologies between the cDNA sequences of human genes and the ESTs sequences of pig,and the high homologous records were screened out. Four Java programs were developed to retrieve and collect sequences, and analyze the BLAST alignment results. By statistical analysis, it was found that there were at least 2002 genes expressed in the fat and muscle tissues of pig, and 1087 in the fat tissue, 1205 in the muscle respectively (290 genes co-expressed in the two tissues). The top-ranking records were screened out,and meantime, 114 basic active genes (BAGs) were found to express in the two tissues,80 in the fat tissue and 34 in the muscle tissue respectively. The top 10 records were described in the paper. This study was summarized in relation to current meat quality improvement of pig at molecular level.
Pan
Zeng-Xiang
ZX
College of animal science and technology Nanjing Agricultural University, Nanjing 210095, China.
Chen
Jie
J
Huang
Rui-Hua
RH
Xu
Dan
D
Xu
Yin-Xue
YX
Xie
Zhuang
Z
Jiang
Zhi-Hua
ZH
Liu
Hong-Lin
HL
Zhao
Ru-Qian
RQ
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D009132:Muscles / Q000378:metabolism*; D013552:Swine / Q000235:genetics*