Flow-injection resonance light scattering detection of proteins at the nanogram level.
A resonance light scattering (RLS) detection method for protein was developed, using a flow-injection system based on the enhancement of RLS signals from Biebrich scarlet (BS) by protein. The enhanced RLS intensities at 286.0 nm, in acidic aqueous medium, were proportional to the protein concentration over the range 0.005-18 microg/mL and 0.008-16 microg/mL for human serum albumin (HSA) and bovine serum albumin (BSA), respectively, with corresponding limits of detection (3sigma) of 5.00 ng/mL for HSA, and 7.80 ng/mL for BSA. The method was successfully applied to the quantification of total proteins in human serum samples.
Tan
Kejun
K
College of Chemistry and Chemical Engineering, and Key Laboratory of Eco-Environments of Three Gorges Reservoir Region under the Ministry of Education of PRC, Southwest Normal University, Chongqing 400715, People's Republic of China.
Li
Yuanfang
Y
Huang
Chengzhi
C
D000818:Animals; D002138:Calibration; D004867:Equipment Design; D006801:Humans; D036103:Nanotechnology; D011506:Proteins / Q000032:analysis* / Q000592:standards; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D014466:Ultraviolet Rays
D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine