A new simply and effective fractionation method for cylindrospermopsin analyses.
A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50% methanol containing 1% formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification.
Kubo
Takuya
T
Graduate School of Environmental Studies, Tohoku University, Aramaki-aza aoba 6-6-20, Aoba-ku, Sendai 980-8579, Japan. kubo@mail.kankyo.tohoku.ac.jp
Sano
Tomoharu
T
Hosoya
Ken
K
Tanaka
Nobuo
N
Kaya
Kunimitsu
K
D000470:Alkaloids; D001427:Bacterial Toxins; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid; D000087522:Cyanobacteria Toxins; D046891:Cylindrospermopsis / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis
D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil