On-line preconcentration/determination of copper in parenteral solutions using activated carbon by inductively coupled plasma optical emission spectrometry.
A method for the on-line preconcentration of copper using a minicolumn packed with activated carbon and its subsequent determination by inductively coupled plasma optical emission spectrometry (ICP-OES) coupled with flow injection (FI) was studied. In order to determinate the copper concentration present in parenteral solutions; it was retained on activated carbon (AC) at pH 9.5. A sensitivity enrichment factor of 30-fold was obtained with respect to the copper determination by ICP-OES without preconcentration. The detection limit for the preconcentration of 25 ml of sample was 0.1 microgl(-1). The precision for the ten replicate determinations at the 2.5 microgl(-1) Cu level was 3.0% relative standard deviation (R.S.D.), calculated with the peak heights. The calibration graph using the preconcentration method for cooper species was linear with a correlation coefficient of 0.9996 at levels near the detection limits up to at least 200 microgl(-1). The method was successfully applied to the determination of copper in parenteral solutions.
Takara
E A
EA
Department of Analytical Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Chacabuco and Pedernera, P.O. Box 375, 5700 San Luis, Argentina.
Pasini-Cabello
S D
SD
Cerutti
S
S
Gásquez
J A
JA
Martinez
L D
LD
D000042:Absorption; D002021:Buffers; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007263:Infusions, Parenteral / Q000295:instrumentation*; D015203:Reproducibility of Results; D012306:Risk; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013678:Technology, Pharmaceutical; D013997:Time Factors
D002021:Buffers; D002244:Carbon; D003300:Copper