[The expression of KDR and VEGF in yolk sac and liver of human embryo].
OBJECTIVE
To study the expression and distribution of KDR, VEGF and CD34 in yolk sac and liver of human embryo at different development stage.


METHODS
Yolk sacs and livers of 15 human embryos were analyzed by the immunohistochemical SP kits for the expression of KDR, VEGF and CD34.


RESULTS
KDR, VEGF and CD34 were all expressed in yolk sacs and livers of the embryos. In the intermediate liver group, the grey value of KDR and VEGF were 103.8 +/- 6.1 and 96.4 +/- 6.3, respectively, stronger than that in the late liver group which were 90.4 +/- 6.0 and 87.4 +/- 6.3, respectively (P < 0.05). A positive correlation between the levels of KDR and VEGF was observed (P < 0.05).


CONCLUSIONS
The expression of KDR and CD34 in yolk sac and liver of embryo suggests the presence of hemangioblast in these organs. Interaction of KDR and VEGF might relate to survival, proliferation, migration and differentiation of hemangioblasts.
Wang
Hai-yan
HY
Shandong Research Center of Stem Cell Engineering, Yantai 264000, China.
Wang
Yue-si
YS
Fan
Guang-li
GL
Meng
Fan-gang
FG
Li
Jian-yuan
JY
D004622:Embryo, Mammalian / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis*; D015017:Yolk Sac / Q000378:metabolism*
D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2