[Protective effect of estrogens replacement on apoptosis cells of nasal mocosas in ovariectomized rats].
OBJECTIVE
To study the protective effect of nylestriol on apoptosis cells of atrophic nasal mucosas in ovariectomized rats and the difference between nasal drip and gastrogavage.


METHODS
Sixty rats with atrophic rhinitis were divided into four groups at random (each with 15 rats): contrary group (Cg), ovariectomized group (Og), ovariectomized + nylestriol nasal drip group (ONNDg), and ovariectomized + nylestriol by gastrogavage (ONGg). Earlier apoptosis cells of nasal mucosas taken from nasal septum were measured with flow cytometry.


RESULTS
After being ovariectomized, the number of apoptosis cells of mucosas increased. In ONGg, the number of apoptosis cells of mucosas increased at 15 d after operation (P < 0.05), but it recovered at 30 d and 60 d after operation. In ONGg, the number of apoptosis cells of mucosas increased at 15 d and 30 d after operation (P < 0.05), but it recovered at 60 d after operation.


CONCLUSIONS
Estrogen replacement by gastrogavage and via nasal drip have effects on protecting cells of mucosas from lacking of estrogen by decreasing apoptosis cells in ovariectomized rats. It might cost more time to get therapeutic effectiveness via nasal drip than by gastrogavage.
Qi
Benming
B
Department of Otolaryngology, the First People's Hospital of Yunnan Province, Kunming, 650001, China. qibenming@21cn.com
Fan
Li
L
Jiang
Hequn
H
Zhang
Lin
L
He
Xiupei
X
Wan
Tao
T
Cai
Chunchun
C
Deng
Xiaoming
X
Tian
Xinghua
X
D000281:Administration, Intranasal; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D015914:Estrogen Replacement Therapy; D005260:Female; D009297:Nasal Mucosa / Q000187:drug effects* / Q000473:pathology; D010052:Ovariectomy; D011800:Quinestrol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
C010722:nylestriol; D011800:Quinestrol