MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains.
Twenty Campylobacter jejuni and 16 Campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. A combined mismatch amplification mutation assay-PCR technique was developed to detect the mutations A 2074 C and A 2075 G in the 23S rRNA gene associated with erythromycin resistance. All high-level erythromycin-resistant strains examined by DNA sequencing carried the transition mutation A 2075 G, whereas no isolate carried the A 2074 C mutation. No mutations were found among the susceptible and low-level erythromycin-resistant strains.
Alonso
Rodrigo
R
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
Mateo
Estibaliz
E
Churruca
Estibaliz
E
Martinez
Irati
I
Girbau
Cecilia
C
Fernández-Astorga
Aurora
A
D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics; D002645:Chickens; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011202:Poultry Products / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013552:Swine
D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D004917:Erythromycin