In silico prediction of binding of putative antigenic peptides to HLA-DRB1 alleles in Vogt-Koyanagi-Harada disease.
We evaluated peptides demonstrated to be immunogenic in studies of HLA-DRB1*0405-restricted T cells from patients with Vogt-Koyanagi-Harada (VKH) disease for likelihood of binding to HLA-DRB1*0405 using internet-accessible algorithms. Immunogenic peptides were more likely to overlap with fragments predicted to have a high likelihood of binding to HLA-DRB1*0405 and were more likely to bind to HLA-DRB1*0405 than the negative control, HLA-DRB1*0701. We also determined the likelihood of binding to other HLA molecules associated with VKH disease. The peptide immunogenic in the most patients was predicted to bind to HLA-DRB1*0101, 0404, and 0405, but not to the negative control, HLA-DRB1*0701. In silico methods hold promise for high-throughput evaluation of putative auto-antigens, for testing and generating hypotheses, and suggest a correlation between HLA binding and immunogenicity.
Prasad
Pradeep S
PS
Ocular Inflammatory Disease Center, Jules Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine, 100 Stein Plaza, UCLA, Los Angeles, CA 90095-7002, USA.
Levinson
Ralph D
RD
D000465:Algorithms; D000483:Alleles; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D015234:HLA-A Antigens / Q000276:immunology / Q000378:metabolism; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D059811:HLA-DRB1 Chains; D006801:Humans; D020407:Internet; D008562:Membrane Glycoproteins / Q000276:immunology; D018448:Models, Immunological; D014442:Monophenol Monooxygenase / Q000276:immunology; D010088:Oxidoreductases / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D014607:Uveomeningoencephalitic Syndrome / Q000235:genetics / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; C488436:HLA DRB1*04:04 antigen; D015234:HLA-A Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C487961:HLA-DRB1*01:01 antigen; C493960:HLA-DRB1*04:05 antigen; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D010088:Oxidoreductases; C489799:TYRP1 protein, human; D014442:Monophenol Monooxygenase