[Role of ROK in contraction of rat femoral arteries after 14 d tail suspension].
OBJECTIVE
To determine the role of Rho associated kinase (ROK) in contraction of rat femoral arteries after 14 d tail suspension.


METHODS
Male Wistar rats were randomly divided into control group (CON) and 14 d -30 degrees tail suspension group (TS14d). Analysis was performed on the contractile responses of perfused femoral arterial rings from both TS14d and CON rats to KCl, phenylephrine (PE), and U-46619 in the presence of Y-27632.


RESULTS
Arterial rings from TS14d rats displayed a reduced contractile response to KCl and PE but not significantly to U-46619. In the response to KCl, Y-27632 caused a concentration-dependent relaxation and significantly larger decrease of contraction in tissues from TS14d rats. Y27632 nearly abolished the tonic component of KCl-induced contraction when its dose was increased from 10(-6)mol/L to 10(-5) mol/L. It had also an inhibitive effect on the PE and U-46619-induced contraction and caused significantly larger decrease in U-46619 but not in KCl or PE induced contraction in tissues from TS14d rats.


CONCLUSIONS
ROK activity may be enhanced and play a compensational role in rat femoral arteries after TS14d.
Li
Zhi-li
ZL
Yuan
Ming
M
Jiang
Shi-zhong
SZ
Wang
De-sheng
DS
Wang
Hui-juan
HJ
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology*; D000577:Amides / Q000494:pharmacology; D000818:Animals; D001835:Body Weight; D005263:Femoral Artery / Q000187:drug effects; D019417:Hindlimb Suspension; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D018474:Weightlessness Simulation; D054460:rho-Associated Kinases
D000577:Amides; D047908:Intracellular Signaling Peptides and Proteins; D011725:Pyridines; D014662:Vasoconstrictor Agents; C108830:Y 27632; D010656:Phenylephrine; D011189:Potassium Chloride; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases