Notch signaling functions as a binary switch for the determination of glandular and luminal fates of endodermal epithelium during chicken stomach development.
During development of the chicken proventriculus (glandular stomach), gut endoderm differentiates into glandular and luminal epithelium. We found that Delta1-expressing cells, undifferentiated cells and Notch-activated cells colocalize within the endodermal epithelium during early gland formation. Inhibition of Notch signaling using Numb or dominant-negative form of Su(H) resulted in a luminal differentiation, while forced activation of Notch signaling promoted the specification of immature glandular cells, but prevented the subsequent differentiation and the invagination of the glands. These results suggest that Delta1-mediated Notch signaling among endodermal cells functions as a binary switch for determination of glandular and luminal fates, and regulates patterned differentiation of glands in the chicken proventriculus.
Matsuda
Yoshimasa
Y
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachiohji, Tokyo 192-0397, Japan.
Wakamatsu
Yoshio
Y
Kohyama
Jun
J
Okano
Hideyuki
H
Fukuda
Kimiko
K
Yasugi
Sadao
S
D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D029721:Drosophila Proteins; D004707:Endoderm / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051880:Receptors, Notch; D015398:Signal Transduction; D013278:Stomach, Avian / Q000196:embryology* / Q000378:metabolism*; D013997:Time Factors
D029721:Drosophila Proteins; D007605:Juvenile Hormones; D008565:Membrane Proteins; D051880:Receptors, Notch; C060606:numb protein, Drosophila