Hearing impairment in TRPV4 knockout mice.
Transient receptor potential channel vanilloid subfamily 4 (TRPV4), a member of TRP family, is a mechanosensitive non-selective cation channel. To investigate the role of TRPV4 in the cochlea, the hearing thresholds and effects of acoustic overexposure on the cochlea were examined in TRPV4 knockout mice. TRPV4 knockout mice at age 8 weeks exhibited normal, but those at 24 weeks revealed significantly higher thresholds by auditory brainstem response. The auditory threshold shift was significantly larger in the TRPV4 knockout than in the TRPV4+/+ mice 1 week after the acoustic overexposure of 128dB SPL. The present findings suggest that disruption of TRPV4 causes delayed-onset hearing loss and makes the cochlea vulnerable to acoustic injury.
Tabuchi
Keiji
K
Department of Otolaryngology, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan. ktabuchi@md.tsukuba.ac.jp
Suzuki
Makoto
M
Mizuno
Atsuko
A
Hara
Akira
A
D000161:Acoustic Stimulation; D000818:Animals; D001303:Auditory Cortex / Q000503:physiopathology*; D001309:Auditory Threshold / Q000502:physiology; D027682:Cation Transport Proteins / Q000235:genetics*; D003051:Cochlea / Q000473:pathology*; D005260:Female; D006311:Hearing Disorders / Q000235:genetics*; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D050916:TRPV Cation Channels
D027682:Cation Transport Proteins; D007473:Ion Channels; D050916:TRPV Cation Channels; C486576:Trpv4 protein, mouse