Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis.
The authors examined the usefulness of nested PCR (N-PCR) to detect Mycobacterium tuberculosis (MTB) DNA in CSF for assessing the clinical course of tuberculous meningitis (TBM). N-PCR successfully detected MTB DNA in all nine CSF samples from patients with suspected TBM. During anti-tuberculosis treatments, N-PCR results converted from positive to negative, correlating with the improvement of the patient's clinical condition.
Takahashi
T
T
Division of Neurology, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan.
Nakayama
T
T
Tamura
M
M
Ogawa
K
K
Tsuda
H
H
Morita
A
A
Hara
M
M
Togo
M
M
Shiota
H
H
Suzuki
Y
Y
Minami
M
M
Ishikawa
H
H
Miki
K
K
Shikata
E
E
Takahashi
S
S
Kuragano
T
T
Matsumoto
K
K
Sawada
S
S
Mizutani
T
T
D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000627:therapeutic use; D002555:Cerebrospinal Fluid / Q000737:chemistry* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000639:trends; D011237:Predictive Value of Tests; D016896:Treatment Outcome; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000382:microbiology
D000995:Antitubercular Agents; D004269:DNA, Bacterial