Cellulase production by Trichoderma reesei using sawdust hydrolysate.
Sawdust hydrolysates were investigated for their ability to support cell growth and cellulase production, and for potential inhibition of Trichoderma reesei Rut C30. Simultaneous fermentations were conducted to compare the hydrolysate-based media with the controls having equivalent concentrations of glucose and Avicel cellulose. Six hydrolysates differing in the boiling durations in the hydrolysis procedure were evaluated. The hydrolysates were found to support cell growth and induce active cellulase synthesis. The maximum specific cellulase production rate was 0.046 filter paper units (FPU)/(g of cells.h) in the hydrolysate-based systems, much higher than that (0.017 FPU/[g of cells.h]) in the controls.
Lo
Chi-Ming
CM
Department of Chemical Engineering, The University of Akron, Akron, OH 44325-3906, USA.
Zhang
Qin
Q
Lee
Patrick
P
Ju
Lu-Kwang
LK
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002480:Cellulase / Q000096:biosynthesis*; D004391:Dust; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D012037:Refuse Disposal; D014197:Trees / Q000737:chemistry / Q000382:microbiology*; D014242:Trichoderma / Q000254:growth & development* / Q000378:metabolism*; D014934:Wood
D004391:Dust; D007220:Industrial Waste; D002480:Cellulase