Genomic Comparison of cag pathogenicity island (PAI)-positive and -negative Helicobacter pylori strains: identification of novel markers for cag PAI-positive strains.
In an analysis of Helicobacter pylori genomic DNA by macroarray methodology, genomic DNA from a panel of cag pathogenicity island (PAI)-negative H. pylori clinical isolates failed to hybridize with 27 genes located outside the cag PAI in a cag PAI-positive reference strain. PCR analyses confirmed that HP0217 (encoding a lipopolysaccharide biosynthetic protein) and HP1079 (encoding a protein of unknown function) were present significantly more frequently in cagA-positive strains than in cagA-negative strains. A low G+C content of these two genes suggests they were acquired by horizontal transfer events.
Terry
Courtney E
CE
Department of Biology, The College of William & Mary, 214 Millington Hall, Landrum Drive, Williamsburg, VA 23187-8795, USA.
McGinnis
Lisa M
LM
Madigan
Katharine C
KC
Cao
Ping
P
Cover
Timothy L
TL
Liechti
George W
GW
Peek
Richard M
RM
Forsyth
Mark H
MH
D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015415:Biomarkers; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D044404:Genomic Islands; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; C080866:cagA protein, Helicobacter pylori