Selection of hypervariable microsatellite loci for the characterization of Saccharomyces cerevisiae strains.
A survey of the genetic polyphormism that can be detected at different microsatellite loci in Saccharomyces cerevisiae strains was performed in order to develop an efficient microsatellite based typing technique. Using gel electrophoresis, we analyzed 41 microsatellite loci for 10 strains and observed that 29 loci displayed significant polymorphism. The measurement of the PCR amplicon size of 21 loci for 46 strains by capillary electrophoresis, allowed us to retain the six most variable sites for S. cerevisiae typing from which three were described for the first time. The resulting combination of these six loci provides a highly discriminant and reproducible technique that also reveals relationships between strains, especially those of particular geographical (or technological) origins. These polymorphic loci can easily be used for PCR profiling of S. cerevisiae strains during fermentation.
Legras
Jean-Luc
JL
Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche Vigne et Vins d'Alsace, 28 rue de Herrlisheim, BP 507, 68021 Colmar Cedex, France. legras@colmar.inra.fr
Ruh
Olivier
O
Merdinoglu
Didier
D
Karst
Francis
F
D000483:Alleles; D001483:Base Sequence; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000032:analysis; D005784:Gene Amplification; D014644:Genetic Variation; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal