title: Allopregnanolone and pregnanolone are produced by the human corpus luteum.
abstract: Using a dispersed human luteal cell culture model, progesterone, allopregnanolone and pregnanolone release following treatment by incremental doses of human chorionic gonadotrophin (hCG) were evaluated. Corpus luteum tissues, obtained from 48 healthy women scheduled for benign surgery, were grouped according to luteal age and tissue concentration of allopregnanolone and pregnanolone was determined. The mRNA expression of 5alpha-, and 5beta-reductase and 3alpha-HSOR mRNA expressions were evaluated in corpora lutea from the late luteal phase. Allopregnanolone concentrations in corpus luteum tissue were consistently about three- to four-fold higher than pregnanolone levels. Allopregnanolone tissue concentrations significantly decreased between early- and late-luteal phase, p<0.05. When exposed to hCG, progesterone output from freshly obtained human corpora lutea cells was two- three-fold increased compared to control levels. With 0.1U/ml hCG a two-fold increase in allopregnanolone levels were noted, whereas pregnanolone levels were increased by approximately 40%. Furthermore, the mRNA of 5alpha-, 5beta-reductase and 3alpha-HSOR mRNA were all expressed in human corpus luteum. In conclusion, the neurosteroids allopregnanolone and pregnanolone are produced in the human corpus luteum and their release is stimulated by trophic hormone.
authors.affiliation: Department of Clinical Science, Obstetrics and Gynecology, Umeå University Hospital, Umeå, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Ottander
authors.forename: Inger Sundström
authors.initials: IS
authors.lastname: Poromaa
authors.forename: Erik
authors.initials: E
authors.lastname: Bjurulf
authors.forename: Asa
authors.initials: A
authors.lastname: Skytt
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Bäckström
authors.forename: Jan I
authors.initials: JI
authors.lastname: Olofsson
mesh_terms: D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D011280:Pregnanolone / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D046509:Tissue Culture Techniques
chemical_list: D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D011280:Pregnanolone; D010088:Oxidoreductases