title: The research on biotransformation of praziquantel.
abstract: Praziquantel (PZQ), a broad spectrum antihelmintic drug, is extensively metabolized in the liver, yielding mainly monohydroxylated and dihydroxylated phase-I-metabolites. However, the exact chemical structures of the most metabolites are still unknown. In the presented research, three types of PZQ biotransformations were performed: biotransformation with the whole cells of Saccharomyces cerevisiae, with cytochrome c from Saccharomyces cerevisiae and with microsomes isolated from Saccharomyces cerevisiae. To describe the obtained metabolites TLC, RP-TLC, and HPLC were used.
authors.affiliation: Jagiellonian University Medical College, Department of Technology and Biotechnology of Drugs, 9 Medyczna Str., PL 30-688 Kraków.
authors.forename: Anna
authors.initials: A
authors.lastname: Godawska-Matysik
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pekala
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kieć-Kononowicz
mesh_terms: D000980:Antiplatyhelmintic Agents / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D045304:Cytochromes c / Q000378:metabolism; D007202:Indicators and Reagents; D011223:Praziquantel / Q000493:pharmacokinetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D000980:Antiplatyhelmintic Agents; D007202:Indicators and Reagents; D011223:Praziquantel; D045304:Cytochromes c