Nonisothermal bioreactors in the treatment of vegetation waters from olive oil: laccase versus syringic acid as bioremediation model.
Laccase from Trametes versicolor was immobilized by diazotization on a nylon membrane grafted with glycidil methacrylate, using phenylenediamine as spacer and coupling agent. The behavior of these enzyme derivatives was studied under isothermal and nonisothermal conditions by using syringic acid as substrate, in view of the employment of these membranes in processes of detoxification of vegetation waters from olive oil mills. The pH and temperature dependence of catalytic activity under isothermal conditions has shown that these membranes can be usefully employed under extreme pH and temperatures. When employed under nonisothermal conditions, the membranes exhibited an increase of catalytic activity linearly proportional to the applied transmembrane temperature difference. Percentage activity increases ranging from 62% to 18% were found in the range of syringic acid concentration from 0.02 to 0.8 mM, when a difference of 1 degrees C was applied across the catalytic membrane. Because the percentage activity increase is strictly related to the reduction of the production times, the technology of nonisothermal bioreactors has been demonstrated to be an useful tool also in the treatment of vegetation waters from olive oil mills.
Attanasio
Angelina
A
Department of Experimental Medicine, Faculty of Medicine and Surgery, Second University of Naples, Via S.M. di Costantinopoli 16, 80136 Naples, Italy.
Diano
Nadia
N
Grano
Valentina
V
Sicuranza
Stefano
S
Rossi
Sergio
S
Bencivenga
Umberto
U
Fraconte
Luigi
L
Di Martino
Silvana
S
Canciglia
Paolo
P
Mita
Damiano Gustavo
DG
D000383:Agriculture / Q000379:methods; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002384:Catalysis; D003198:Computer Simulation; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control; D042845:Laccase / Q000737:chemistry*; D008567:Membranes, Artificial; D008956:Models, Chemical; D000069463:Olive Oil; D010636:Phenols / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D013696:Temperature; D018508:Water Purification / Q000379:methods
D004800:Enzymes, Immobilized; D007220:Industrial Waste; D008567:Membranes, Artificial; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D005707:Gallic Acid; C001945:syringic acid; D042845:Laccase