[An experimental study on injury of hippocampus neurons in rats by simulated push-pull maneuver of different degrees].
OBJECTIVE
To explore the degree of injury and phase of push-pull effect induced by different degrees of push-pull maneuver on hippocampus neurons in rats.


METHODS
Injury of push-pull maneuver on hippocampus neurons in rats after different degrees of push-pull maneuver were determined by general evaluation of animal model, pathologic examination and electronic microscopy.


RESULTS
1) General evaluation of animal model: the apoplexy index and the pathological grading increased with the increase of intensity of the push-pull maneuver. 2) Routine HE: No abnormity was found in appearance and distribution of hippocampus neurons in the control rats and various groups of +/-2 Gz exposures, and 30 min groups of +/-6 Gz, +/-8 Gz exposures, but denatured necrosis of neurons were found in 6 h and 24 h groups of +/-6 Gz, +/-8 Gz exposures. 3) Examination of ultrastructure under electronic microscope: No significant change were found in hippocampus neurons of CA1 area in 30 min and 24 h after +/-2 Gz exposures as compared with the normal control group. But various degrees of denatured necrosis of neurons were found in some of the hippocampus neurons of CA1 area in 6 h after +/-2 Gz exposures, 6 h and 24 h after +/-6 Gz and +/-8 Gz exposures.


CONCLUSIONS
+/-6 Gz and +/-8 Gz exposures may cause injury of hippocampus tissues and it is most severe at 6 h after the +/-8 Gz exposures.
Guo
Su-hong
SH
Jilin Military Medical College, The Fourth Military Medical University, Jilin, China.
Wang
Xiao-yu
XY
Dou
Zhao-hua
ZH
Yao
Ping
P
Zhang
Hui
H
Li
Yun-yi
YY
D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology; D004195:Disease Models, Animal; D006624:Hippocampus / Q000293:injuries / Q000473:pathology*; D018471:Hypergravity / Q000009:adverse effects*; D008854:Microscopy, Electron; D009336:Necrosis; D009474:Neurons / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000209:etiology / Q000473:pathology; D013997:Time Factors