Collagen-coated polylactide microspheres as chondrocyte microcarriers.
Polylactide (PLA) microspheres were coated with collagen for cell culture and injectable cell carriers. Utilizing a method of emulsion-solvent evaporation, PLA microspheres with diameter ranging from 180 to 280 microm were prepared, followed with aminolysis in hexanediamine/n-propanol solution to introduce free amino groups on their surfaces. After the amino groups were transferred into aldehyde groups by a treatment of glutaraldehyde, collagen type I was covalently coupled via Schiff base formation between the aldehyde groups and the amino groups on collagen molecules. Meanwhile, physically entangled collagen molecules were retained following a grafting-coating protocol to yield microspheres coated with larger amount of collagen. Aminolysis resulted in weight loss of the microspheres following a linear relationship with the aminolysis time. The NH2 and collagen contents existed on the microsphere surface were quantitatively determined by ninhydrin and hydroproline (Hyp) analyses, respectively. Larger amount of collagen was immobilized on the microspheres with higher content of NH2. In vitro chondrocyte culture revealed that the cells could attach, proliferate and spread on these PLA microspheres, in particular on the ones having higher content of collagen. These results show that the collagen-coated PLA microspheres are promising candidate as cell microcarriers.
Hong
Yi
Y
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Gao
Changyou
C
Xie
Ying
Y
Gong
Yihong
Y
Shen
Jiacong
J
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits
D020099:Coated Materials, Biocompatible; D011091:Polyesters; C033616:poly(lactide); D003094:Collagen