CYP35: xenobiotically induced gene expression in the nematode Caenorhabditis elegans.
Although over 80 cytochrome P450 (CYP) encoding genes have been identified in the genome of the nematode Caenorhabditis elegans very little is known about their involvement in biotransformation. This paper demonstrates a concentration-dependent relationship of C. elegans CYP35A1, A2, A5, and C1 gene expression in response to four organic xenobiotics, namely atrazine, PCB52, fluoranthene, and lansoprazole. The toxicity of these xenobiotics was determined using a reproduction assay. CYP-specific messenger RNA expression was analyzed by semi-quantitative RT-PCR resulting in a strongly increasing, concentration-dependent induction well below the EC50 for reproduction. For PCB52, approximately 0.5% of the EC50 induces a 2-fold increase of CYP35 gene expression. Using a double mutant and multiple RNAi of CYP35A/C it was possible to diminish the reproduction decline caused by PCB52 and fluoranthene.
Menzel
Ralph
R
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany. ramenzel@igb-berlin.de
Rödel
Matthias
M
Kulas
Jana
J
Steinberg
Christian E W
CE
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D001280:Atrazine / Q000633:toxicity; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005449:Fluorenes / Q000633:toxicity; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D064747:Lansoprazole; D009853:Omeprazole / Q000031:analogs & derivatives* / Q000633:toxicity; D011078:Polychlorinated Biphenyls / Q000633:toxicity; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology*; D015262:Xenobiotics / Q000633:toxicity*
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; C502396:CYP35 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D005449:Fluorenes; D015262:Xenobiotics; D064747:Lansoprazole; C007738:fluoranthene; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D009853:Omeprazole; D001280:Atrazine