title: Dephosphorylation and internalization of cell adhesion molecule L1 induced by theta burst stimulation in rat hippocampus.
abstract: The neural cell adhesion molecule L1 may participate in initiating and maintaining synaptic changes during learning in the hippocampus. One prominent form of synaptic change in the hippocampus is long-term potentiation (LTP) that occurs following specific patterns of synaptic activity. We present evidence that Y1176 of the YRSL motif within L1 cytoplasmic domain is dephosphorylated in LTP-induced hippocampus. The dephosphorylated L1 is associated with AP-2 and AP180 that are required for clathrin-mediated internalization of L1. These data suggest that clathrin-mediated recycling of L1 at presynaptic sites is enhanced by certain kinds of neural activity, and that maintenance of LTP-induced synaptic changes is regulated by L1 recycling.
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, Kagawa 769-2193, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Itoh
authors.forename: Ken
authors.initials: K
authors.lastname: Shimono
authors.forename: Vance
authors.initials: V
authors.lastname: Lemmon
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D004558:Electric Stimulation; D004705:Endocytosis / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D017774:Long-Term Potentiation / Q000502:physiology*; D034041:Monomeric Clathrin Assembly Proteins / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D009924:Organ Culture Techniques; D010766:Phosphorylation; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000378:metabolism*; D013826:Theta Rhythm
chemical_list: D033962:Adaptor Protein Complex 2; D034041:Monomeric Clathrin Assembly Proteins; D039842:Neural Cell Adhesion Molecule L1; C439023:clathrin assembly protein AP180