Spectrophotometric determination of DNA using a near infrared probe 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine.
A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris-HCl buffer, the reaction of DSTCY with DNA was complete within 10 min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5-8.0 microg ml(-1) and the detection limit of 45 ng ml(-1).
Wang
Hong
H
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Li
Wei-Rong
WR
Lu
Yin
Y
Fu
Ni-Na
NN
Zhang
Hua-Shan
HS
D002021:Buffers; D002232:Carbocyanines / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared; D013696:Temperature
C479728:1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine; D002021:Buffers; D002232:Carbocyanines; D002593:Cetrimonium Compounds; D004247:DNA; C427203:calf thymus DNA; D000077286:Cetrimonium