Cell microarrays and RNA interference chip away at gene function.
The recent development of cell microarrays offers the potential to accelerate high-throughput functional genetic studies. The widespread use of RNA interference (RNAi) has prompted several groups to fabricate RNAi cell microarrays that make possible discrete, in-parallel transfection with thousands of RNAi reagents on a microarray slide. Though still a budding technology, RNAi cell microarrays promise to increase the efficiency, economy and ease of genome-wide RNAi screens in metazoan cells.
Wheeler
Douglas B
DB
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Carpenter
Anne E
AE
Sabatini
David M
DM
D000818:Animals; D002478:Cells, Cultured; D020868:Gene Silencing; D023281:Genomics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000639:trends; D034622:RNA Interference