Herpes simplex virus 1 inhibits apoptosis through a caspase-3-dependent pathway in primary cultures of cortical neuronal cells of fetal mice.
We could induce apoptosis in primary cultures of cortical neurons of fetal mice with ceramide or sorbitol. The induction was accompanied by an increase in caspase-3 (CAS-3) activity and depolarization of the inner mitochondrial membrane of neuronal cells which both could be reversed by Herpes simplex virus 1 (HSV-1) infection. We conclude tha HSV-1 infection inhibited the apoptosis, induced in neuronal cells by sorbitol or ceramide, via a CAS-dependent pathway.
Wang
J W
JW
Department of Neurology, Beijing Friendship Hospital-Affiliate of Capital University of Medical Sciences, Beijing 100050, PR China.
Wang
D X
DX
Wang
R J
RJ
Li
W R
WR
Tuo
H Z
HZ
Feng
Z J
ZJ
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D002518:Ceramides / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D013012:Sorbitol / Q000494:pharmacology
D061945:Caspase Inhibitors; D002518:Ceramides; D013012:Sorbitol; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases