Chemiluminescence evaluation of oxidative damage to biomolecules induced by singlet oxygen and the protective effects of antioxidants.
A chemiluminescence (CL) method was developed for the evaluation of oxidative damage to biomolecules induced by singlet oxygen ((1)O(2)) and for the evaluation of the protective effects of antioxidants. The (1)O(2) was generated from the reaction of H(2)O(2)+OCl(-). Results showed that the CL signal from the reaction of H(2)O(2)+OCl(-) was weak, however, it was enhanced dose-dependently with the addition of DNA and unsaturated fatty acid, respectively. Spectra analysis indicated that the enhanced CL could be ascribed to the decay of triplet-excited carbonyl compounds, which were generated from the reaction of (1)O(2) plus the biomolecules. On the other hand, the enhanced CL produced in the above systems could be effectively inhibited by lycopene, beta-carotene, VC, and VE, but could not be inhibited by mannitol, SOD, and NaN(3). The mechanism therein was discussed.
Wenli
Yu
Y
School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai 200240, PR China. yuwenli@sjtu.edu.cn
Yaping
Zhao
Z
D000975:Antioxidants / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D020011:Protective Agents / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry*
D000975:Antioxidants; D020011:Protective Agents; D026082:Singlet Oxygen; D004247:DNA; D019787:Linoleic Acid