Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing.
OBJECTIVE
The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressings: Mepitel and Tulle Gras.


METHODS
Proliferation was measured by the extent of thymidine incorporation into the replicating DNA of proliferative fibroblasts in contact with the complete dressing.Additional cell viability and metabolism were evaluated using MTT assay. Morphology and ultrastructure analysis were based on immunolabelling and confocal laser microscopy.


RESULTS
Only Urgotul significantly stimulated thymidine incorporation, generally with a maximal proliferative effect at a contact time of 48 hours. This was confirmed by the observation of a greater number of dividing cells (mitotic cells) than in the control cultures. No cytotoxicity was observed following treatment with this dressing. Cells exhibited normal structural and ultrastructural features.


CONCLUSIONS
Fibroblasts play a key role in dermal wound repair. The ability of Urgotul to promote fibroblast proliferation could explain its efficiency in the healing process of acute and chronic wounds.


BACKGROUND
This study was supported by Urgo Laboratories.
Bernard
F X
FX
BIOalternatives SAS, Gençay, France.
Barrault
C
C
Juchaux
F
F
Laurensou
C
C
Apert
L
L
D000704:Analysis of Variance; D047070:Bandages, Hydrocolloid; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003102:Colloids / Q000494:pharmacology*; D020405:Dermis / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D015999:Multivariate Analysis; D012680:Sensitivity and Specificity; D012869:Skin Absorption / Q000187:drug effects
D003102:Colloids