Laminin-5 gamma2 chain and matrix metalloproteinase-2 expression in the neoplastic changes of uterine cervical squamous epithelium.
Laminin-5 is an essential component of epithelial basal membranes. Matrix metalloproteinase-2 (MMP-2) changes laminin-5 from static to migratory substrate by cleaving the short arm of the gamma2 subunit of the laminin-5. Recently, the expression of laminin-5 gamma2 chain has been reported to be a marker of invasiveness of epithelial malignant tumors. We investigated the expression of laminin-5 gamma2 chain and MMP-2 in the neoplastic changes of uterine cervical squamous epithelium. Tissue samples of normal uterine cervix, cervical intraepithelial neoplasia-1, -3 (CIN-1, -3) and squamous cell carcinoma keratinizing (SCC-K) and non-keratinizing (SCC-NK) were analyzed for expression of the laminin-5 gamma2 chain and MMP-2 by immunohistochemistry. In normal uterine cervix and CIN-1, laminin-5 gamma2 chain was immunolocalized only in the basal membrane. In CIN-3, expression of laminin-5 gamma2 chain and MMP-2 became apparent at the epithelial-stromal interface of epithelial cells. In SCC-K and SCC-NK, laminin-5 gamma2 chain and MMP-2 expression were abundant at the epithelial-stromal interface of tumor clusters. These results suggest that the expression of laminin-5 gamma2 chain and MMP-2 may be involved in the process of neoplastic changes of uterine cervical squamous epithelium.
Malina
Robin
R
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.
Motoyama
Satoru
S
Hamana
Shinya
S
Maruo
Takeshi
T
D001485:Basement Membrane / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002584:Cervix Uteri / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*; D002578:Uterine Cervical Dysplasia / Q000378:metabolism*
C400567:LAMC2 protein, human; D007797:Laminin; D020778:Matrix Metalloproteinase 2