[Effects of triptolide on migration of vascular endothelial cells].
OBJECTIVE
To investigate the inhibitory effects of triptolide on the migration, gene and protein expression of urokinase-type plasminogen activator (u-PA) of endothelial cells (ECs) cultured in vitro and to elucidate the anti-angiogenic mechanism of triptolide.


METHODS
Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and the passage 3 cells were treated with triptolide (0, 5, 10, 20, 30 microg/L). Three-dimensional culture system was used to assess the effect of triptolide on the migration of HUVECs. Real time quantitive reverse transcription polymerase chain reaction and immunohistochemical assay were applied to detect gene and protein expression of u-PA.


RESULTS
Triptolide decreased the migration activity and suppressed the expression of u-PA of HUVECs at the gene and protein level.


CONCLUSIONS
Triptolide could inhibit the migration of ECs by reducing the gene and protein expression of u-PA. These may contribute to an elucidation of the mechanism by which triptolide inhibits the angiogenesis.
Ding
Yi
Y
Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhao
Qing-liang
QL
Li
Yong-gang
YG
Zhang
Jie
J
Wang
Qiu-lin
QL
Wang
Shu-ren
SR
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology*; D004852:Epoxy Compounds; D006801:Humans; D007231:Infant, Newborn; D010616:Phenanthrenes / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics
D020533:Angiogenesis Inhibitors; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D012333:RNA, Messenger; C001899:triptolide; D014568:Urokinase-Type Plasminogen Activator