[The selection and properties of Penicillium verruculosum mutants with enhanced production of cellulases and xylanases].
The paper describes three Penicillium verruculosum 28K mutants with about threefold enhanced production of five industrially important carbohydrases. The two-stage fermentation process that we developed provided a further two- to threefold increase in the production of carbohydrases. Physiological and biochemical studies showed that the synthesis of all five carbohydrases is inducible. Carboxymethylcellulase, xylanase, and beta-glucanase are synthesized under a common regulatory control, as is evident from the concurrent increase in the synthesis of these enzymes in the presence of microcrystalline cellulose. The synthesis of avicelase and beta-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
Solov'eva
I V
IV
Okunev
O N
ON
Vel'kov
V V
VV
Koshelev
A V
AV
Bubnova
T V
TV
Kondrat'eva
E G
EG
Skomarovskiĭ
A A
AA
Sinitsyn
A P
AP
D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism; D009154:Mutation; D010407:Penicillium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012641:Selection, Genetic; D014995:Xylosidases / Q000096:biosynthesis / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
D003470:Culture Media; D002482:Cellulose; D006026:Glycoside Hydrolases; D014995:Xylosidases; C019822:carbohydrase; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase