Development of miniaturized multi-channel high-performance liquid chromatography for high-throughput analysis.
We have developed miniaturized multi-channel high-performance liquid chromatography (HPLC) system. With this system, we can simultaneously separate multiple samples, using a single high-pressure gradient pump, a chip-based sample injection unit, a monolithic silica capillary column array, and a multi-channel UV detection unit based on fiber optics. The injection unit has a simplified structure composed of brass housing and a quartz microchip having microchannels and access ports, which enable a direct injection of sample to multi-channel by commercial multichannel micropipette. Moreover, that possesses a function of microvalve, and on-chip definition of sample injection plugs achieved with a cross channel injection method, providing each column of monolithic silica capillary array. The substances in channels were simultaneously detected with UV having multiple cells. Standard samples were analyzed for characterizing newly developed system, and sharp peaks were obtained with reproducibility data of < 0.9% (R.S.D.). Analysis of tryptic digestion of casein was also employed. These results show that the novel multi-channel HPLC system has the benefits for the high-throughput analysis in the post-genomic analysis/combinatorial chemistry.
Shintani
Yukihiro
Y
GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan. shintani@gls.co.jp
Hirako
Keiji
K
Motokawa
Masanori
M
Iwano
Takeshi
T
Zhou
Xiaojing
X
Takano
Yoshihiko
Y
Furuno
Masahiro
M
Minakuchi
Hiroyoshi
H
Ueda
Mitsuyoshi
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D008904:Miniaturization; D010455:Peptides / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides