title: [Preparation and identification of monoclonal antibody against heterogeneous nuclear ribonucleoprotein B1].
abstract: OBJECTIVE
  To establish anti-heterogeneous nuclear ribonucleoprotein B1 monoclonal antibody cell line and to identify immunological activity.
  
  
  METHODS
  BALB/c mice were immunized with specific hnRNPB1 polypeptide by multiple-place hypodermic injection. The splenic cells of the mice were fused with SP2/0 mouse myeloma cells. The hybridoma culture supernants were screened by indirect ELISA with artificial synthetic peptide.
  
  
  RESULTS
  Two hybridoma cell lines against hnRNPB1 were successfully obtained; the titers of antibody in ascites were 1: 1.8 x 10(5). The McAb showed the character of no cross reactions. Immunohistochemical analysis demonstrated that the antibody could bind with hnRNPB1 specifically.
  
  
  CONCLUSIONS
  The McAb prepared so by this laboratory is of good specificity and sensitivity.
authors.affiliation: Respiratory Department, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Ming-yong
authors.initials: MY
authors.lastname: Chen
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Wei
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D016601:RNA-Binding Proteins