[Gene expression of resistin and the effect of conjugated linoleic acid on its expression of obese rat fed with high fat diet].
OBJECTIVE
To study the gene expression of the resistin and the effects of conjugated linoleic acid on its expression in white adipose tissue of obese rats fed with high fat diet during the formation of insulin resistance.


METHODS
Male Wistar rats were randomly separated in control group, high-fat group and high fat + conjugated linoleic acid (CLA) group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight), using reverse transcription polymerase chain reaction (RT-PCR) technique to measure the expression level of resistin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA expression.


RESULTS
the serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) mIU/L, (5.09 +/- 0.66) mmol/L, and supplement of CLA might decrease hyperinsulinemia and hyperglycemia, in CLA group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight) the serum insulin levels were (6.99 +/- 1.77) mIU/L, (7.36 +/- 1.48) mIU/L, (7.85 +/- 1.60) mIU/L, and glucose levels were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L, (4.06 +/- 0.63) mmol/L. The expression of resistin in adipose tissue of obese rat fed with high fat diet was increased as compared with those fed with basic diet. CLA might increase the expression of resistin and PPARgamma in adipose tissue of obese rat.


CONCLUSIONS
The expression of resistin mRNA of obese rat fed with high fat diet was higher than those fed with basic diet, and CLA might improve the insulin resistance in obese rats and possibly upregulate the expression of resistin through activing PPARgamma.
Zhou
Xiao-rong
XR
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150001, China.
Sun
Chang-hao
CH
Zhang
Xiao-hong
XH
Wang
Hai-ying
HY
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D015870:Gene Expression / Q000187:drug effects*; D007333:Insulin Resistance; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D052243:Resistin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D012333:RNA, Messenger; D052243:Resistin; C046938:9,11-linoleic acid