title: Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor.
abstract: Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Dipterans. Both mdesprot-I and mdesprot-II encoded proteins with secretion signal peptides at the N-terminals, indicating the proteins are secreted proteases that should function as midgut digestive proteases. A cytological analysis with fluorescent in situ hybridization revealed the cytological localization of mdesprot-I and mdesprot-II on the long arm of Autosome 2. Results are discussed in the context of the efficacy of potential protease inhibitors to develop Hessian fly resistant wheat through genetic engineering approaches.
authors.affiliation: Department of Entomology, Purdue University, West Lafayette, IN 47906, USA.
authors.forename: O
authors.initials: O
authors.lastname: Mittapalli
authors.forename: J J
authors.initials: JJ
authors.lastname: Stuart
authors.forename: R H
authors.initials: RH
authors.lastname: Shukle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004064:Digestive System / Q000201:enzymology; D004175:Diptera / Q000201:enzymology* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D010802:Phylogeny; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D012697:Serine Endopeptidases