Resveratrol may reduce oxidative stress induced by platinum compounds in human plasma, blood platelets and lymphocytes.
Resveratrol (trans-3,4',5-trihydroxystilbene), a polyphenolic compound found in grapes and wine, has been shown to have anti-inflammatory, anti-oxidant, anti-tumor and anti-platelet activities. Using different methods, we show that resveratrol reduces oxidative stress induced by cisplatin (cis-diamminedichloroplatinum II) and selenium-cisplatin conjugate ([NH(3)](2)Pt(SeO(3)), Se-Pt) in human blood platelets, lymphocytes and plasma. Resveratrol decreased the production of 8-epi-prostaglandin F(2) (a biomarker of lipid peroxidation) in control blood platelets and platelets treated with platinum compounds (10 microg/ml), and markedly reduced activities of different anti-oxidative enzymes (glutathione peroxidase, superoxide dismutase and catalase) in these cells. A combined action of resveratrol and Se-Pt evoked a significant decrease of DNA damage (measured by comet assay) in lymphocytes compared with cells treated with Se-Pt only. Resveratrol also caused a distinct reduction of total anti-oxidant level in plasma after incubation with platinum compounds. Therefore, anti-oxidative activity of resveratrol may diminish oxidative stress and damage to cellular biomolecules (lipids, proteins and DNA) induced by platinum compounds.
Olas
Beata
B
Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Poland. olasb@biol.uni.lodz.pl
Wachowicz
Barbara
B
Majsterek
Ireneusz
I
Blasiak
Janusz
J
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001769:Blood / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000031:analogs & derivatives / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000097:blood; D010949:Plasma / Q000187:drug effects; D017671:Platinum Compounds / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D000077185:Resveratrol; D018036:Selenium Compounds / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology*
D000970:Antineoplastic Agents; D000975:Antioxidants; D017671:Platinum Compounds; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; D013267:Stilbenes; C110743:selenium-cisplatin conjugate; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D010088:Oxidoreductases; D002945:Cisplatin; D000077185:Resveratrol