A new fluorogenic transformation: development of an optical probe for coenzyme Q.
[reaction: see text] A new fluorogenic transformation based on a quinone reduction/lactonization sequence has been developed and evaluated as a tool for probing redox phenomena in a biochemical context. The probe presented herein is an irreversible redox probe and is reduced selectively by biologically relevant quinols such as ubiquinol but is inert to reduced nicotinamides (e.g., NADH). The ensuing cyclization is fast and quantitative and provides a measurable optical response.
Tremblay
Matthew S
MS
Department of Chemistry, Columbia University, New York, New York 10027, USA.
Sames
Dalibor
D
D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009536:Niacinamide / Q000031:analogs & derivatives / Q000737:chemistry; D010084:Oxidation-Reduction; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry
D005456:Fluorescent Dyes; D014451:Ubiquinone; D009536:Niacinamide; C003741:ubiquinol