Molecular cloning and characterization of a novel human C4orf13 gene, tentatively a member of the sodium bile acid cotransporter family.
By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA (C4orf13). This cDNA is 2706 bp in length, encoding a 340-amino-acid polypeptide that contains a typical SBF (sodium bile acid cotransporter family) domain and ten possible transmembrane segments. The putative protein C4orf13 shows high similarity with its orthologs in Mus musculus and Xenopus laevis. Human C4orf13 is mapped to chromosome 4q31.2 and contains 12 exons. RT-PCR analysis shows that human C4orf13 is widely expressed in human tissues, and the expression levels in liver and lung are relatively high, expression levels in placenta, kidney, spleen, and thymus are moderate, low levels of expression are detected in heart, prostate, and testis.
Zou
Xianqiong
X
School of Resource Processing and Bio-engineering, Central South University, Changsha 410083, PR China.
Wang
Dianzuo
D
Qiu
Guanzhou
G
Ji
Chaoneng
C
Jin
Feng
F
Wu
Maoqing
M
Zheng
Huarui
H
Li
Xin
X
Sun
Liyun
L
Wang
Yu
Y
Tang
Rong
R
Zhao
Robert Chunhua
RC
Mao
Yumin
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D029363:Organic Anion Transporters, Sodium-Dependent / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D027981:Symporters / Q000737:chemistry / Q000235:genetics*; D014982:Xenopus laevis
D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter