[Effective productions of plant secondary metabolites having antitumor activity by plant cell and tissue cultures].
Methods for the effective production of plant secondary metabolites with antitumor activity using plant cell and tissue cultures were developed. The factors in tannin productivity were investigated using culture strains producing different types of hydrolyzable tannins, i.e., gallotannins (mixture of galloylglucoses), ellagi-, and dehydroellagitannins. Production of ellagi- and dehydroellagitannins was affected by the concentrations and ratio of nitrogen sources in the medium. The formation of oligomeric ellagitannins in shoots of Oenothera tetraptera was correlated with the differentiation of tissues. Cultured cells of Eriobotrya japonica producing ursane- and oleanane-type triterpenes with antitumor activities were also established.
Taniguchi
Shoko
S
Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan. taniguchi@pharm.okayama-u.ac.jp
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D031983:Eriobotrya / Q000378:metabolism*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D009369:Neoplasms / Q000473:pathology; D009584:Nitrogen; D031664:Oenothera / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D047348:Hydrolyzable Tannins; D014315:Triterpenes; D009584:Nitrogen