Functional characterization of KlHEM13, a hypoxic gene of Kluyveromyces lactis.
The KlHEM13 gene of Kluyveromyces lactis encoding the coproporphyrinogen oxidase (EC 1.3.3.3), an oxygen-requiring enzyme that catalyzes the sixth step of heme biosynthesis, was cloned and functionally characterized. The coding and upstream regions of KlHEM13 were analyzed and the putative cis regulatory elements were discussed in relation to the mechanisms of regulation of this hypoxic gene in K. lactis.
Blanco
Moisés
M
Departmento de Biología Celular y Molecular, Universidad de A Coruña, F. Ciencias, Spain.
Becerra
Manuel
M
González-Siso
M Isabel
MI
Cerdán
M Esperanza
ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003304:Coproporphyrinogen Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006418:Heme / Q000096:biosynthesis; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins; D006418:Heme; D003304:Coproporphyrinogen Oxidase; D010100:Oxygen