In vitro bioactivity-guided fractionation and characterization of polyphenolic inhibitory fractions from Acacia nilotica (L.) Willd. ex Del.
The present study was undertaken to evaluate antimutagenic and cytotoxic effects of different extracts/fractions of Acacia nilotica prepared by maceration method. The potency order of different extracts was more or less similar in Ames assay as well as in cytotoxic assay. Considering the maximum potential of acetone extract in both the assays, the studies were initiated to fractionate this extract. Two pure fractions, namely AN-1 and AN-2, were obtained from acetone extract, of which AN-2 was found to be of gallic acid and AN-1 fraction is still to be identified. In conclusion, the antimutagenic and cytotoxic activities exhibited by acetone extract may partially be ascribed to the presence of gallic acid and other polyphenols.
Kaur
Kamaljit
K
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India.
Michael
Husheem
H
Arora
Saroj
S
Härkönen
Pirkko
P
Kumar
Subodh
S
D000045:Acacia / Q000737:chemistry*; D000096:Acetone / Q000737:chemistry; D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D010636:Phenols / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D059808:Polyphenols; D013997:Time Factors
D016587:Antimutagenic Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D000096:Acetone; D005707:Gallic Acid