title: Cell-type-dependent activation of the cellular EF-1alpha promoter by the varicella-zoster virus IE63 protein.
abstract: The varicella-zoster virus (VZV) IE63 protein is abundantly expressed during productive viral infection and is one of six gene products that appear to be expressed during latency. We have found that the IE63 protein can activate expression from the cellular EF-1alpha promoter in the absence of other viral proteins. The VZV IE62 protein, in contrast, was not found to transactivate this promoter. These data indicate that IE63 can function independently of the IE62 protein to positively influence the cellular transcription apparatus. We show that IE63 activation of the EF-1alpha promoter is cell type dependent and have examined the effects of point mutations important for IE63 phosphorylation and virus viability on this activation.
authors.affiliation: Department of Microbiology and Immunology and Witebsky Center for Microbial Pathogenesis and Immunology, 138 Farber Hall, University at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Tricia
authors.initials: T
authors.lastname: Zuranski
authors.forename: Hesham
authors.initials: H
authors.lastname: Nawar
authors.forename: Donna
authors.initials: D
authors.lastname: Czechowski
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Lynch
authors.forename: Ann
authors.initials: A
authors.lastname: Arvin
authors.forename: John
authors.initials: J
authors.lastname: Hay
authors.forename: William T
authors.initials: WT
authors.lastname: Ruyechan
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D020648:Peptide Elongation Factor 1; D015534:Trans-Activators; D014759:Viral Envelope Proteins; C074551:immediate early protein 63, Human herpesvirus 3