[Glutathione-deficient state of nervous tissues in starved animals intensifies lipid peroxidation and oxidation of protein SH-groups].
The levels of the lipid peroxidation products (LPO), different forms of protein SH-groups and their oxidation rate in the homogenates of the mesencephalon, hypothalamus and sensorymotor cortex of normal and GSH-deficient rats under 3-day food starvation were studied. It was shown, that the basic level of LPO products--lipid hydroperoxides and malonic dialdehyde (MDA) in hypothalamus and sensorymotor cortex of normal animals are by 20-30% (p < 0.05) higher and reduced glutathione (GSH) content is 2 times higher, than these values in mesencephalon. Under 3 day starvation of normal animals activation of the LPO observed only in the hypothalamus and sensorymotor cortex, whereas under 3 day starvation of the GSH-deficient rats formed by the intraparenteraly injection of diethylmaleate in a dose of 2.5 mmol/kg of body weight in all investigated structures the lipid hydroperoxides and MDA increased many times (2-3 times), the content of the surface and masked protein SH-groups decreased and essentially increased the oxidation rate of these functional groups. It was proposed that GSH and its enzymes participate in the LPO regulation and protection of protein SH-groups from oxidative damage at this event the intensity of this prosesse depends on structural and functional organization of nervous tissues.
Kerimov
B F
BF
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D005508:Food Deprivation; D005978:Glutathione / Q000172:deficiency*; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D013217:Starvation / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013997:Time Factors
D008054:Lipid Peroxides; D009419:Nerve Tissue Proteins; D013438:Sulfhydryl Compounds; D005978:Glutathione