Evaluation of cell culture on the polyurethane-based membrane (Tegaderm): implication for tissue engineering of skin.
BACKGROUND
The use of polymer-based delivery systems, on which cells are cultured and transferred, improves the ease of handling and transfer of the keratinocytes. A transparent polymer also allows observation of cell growth prior to grafting as well as re-epithelialization after grafting to the wound. We have developed techniques for cultured keratinocytes on Tegaderm (3M), an inexpensive and easily available polyurethane-based wound dressing, for treatment of burn and chronic wounds. In this study, we evaluate cell culture characteristics of three different cell types, human epidermal keratinocytes, human dermal fibroblasts and pig bone marrow mesenchymal stem cells on Tegaderm membrane.


METHODS
Cells were isolated from human skin or pig bone marrow and cultured on membranes for a period of five days. Cell proliferation was assessed by colorimetric assay (MTT) and scanning electron microscopy.


CONCLUSIONS
This study confirms that Tegaderm membranes support attachment and growths for these cell types, with those growth characteristics are similar, if not as good as that of optimal condition of tissue culture plastics. Data from our study suggest that Tegaderm membranes can be used, modified and developed further as an economical and easily available material for tissue engineered skin.
Phan
T T
TT
Department of Plastic Surgery, Singapore General Hospital, Republic of Singapore. surptt@nus.edu.sg
Lim
I J
IJ
Tan
E K
EK
Bay
B H
BH
Lee
S T
ST
D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes; D013552:Swine; D023822:Tissue Engineering
D011140:Polyurethanes