Investigation of ligand-receptor systems by high-resolution solid-state NMR: recent progress and perspectives.
Solid-state Nuclear Magnetic Resonance (NMR) provides a general method to study molecular structure and dynamics in a non-crystalline and insoluble environment. We discuss the latest methodological progress to construct 3D molecular structures from solid-state NMR data obtained under magic-angle-spinning conditions. As shown for the neurotensin/NTS-1 system, these methods can be readily applied to the investigation of ligand-binding to G-protein coupled receptors.
Luca
Sorin
S
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health, Bethesda, Maryland 20892, USA.
Heise
Henrike
H
Lange
Adam
A
Baldus
Marc
M
D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000639:trends*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D009496:Neurotensin / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism
D008024:Ligands; D043562:Receptors, G-Protein-Coupled; D009496:Neurotensin