Determination of mercaptopurine and its four metabolites by large-volume sample stacking with polarity switching in capillary electrophoresis.
This study describes approaches for stacking a large volume of sample solutions containing a mixture of mercaptopurine monohydrate, 6-methylmercaptopurine, thioguanine, thioguanosine, and thioxanthine in capillary electrophoresis (CE). After filling the run buffer (60 mM borate buffer, pH 8.5), a large sample volume was loaded by hydrodynamic injection (2.5 psi, 99.9 s), followed by the removal of the large plug of sample matrix from the capillary using polarity switching (-15 kV). Monitoring the current and reversing the polarity when 95% of current recovered, the separation of anionic analytes was performed in a run buffer < 20 kV. Around 44- to 90-fold improvement of sensitivity for five analytes was achieved by large-volume stacking with polarity switching when compared with CE without stacking. This method was feasible for determination of the analytes spiked in plasma. Removing most of electrolytes from plasma is a key step for performing large-volume sample stacking. Solid-phase extraction was used for pretreatment of biological samples. To our knowledge, this study is one of few applications showing the possibilities of this stacking procedure to analyze biological samples by large-volume sample stacking with polarity switching (LVSSPS) in CE.
Wang
Chun-Chi
CC
Graduate Institute of Pharmaceutical Science, College of Pharmacy, Kaohsiung Medical University, Taiwan.
Chiou
Shyh-Shin
SS
Wu
Shou-Mei
SM
D019075:Electrophoresis, Capillary / Q000379:methods*; D006151:Guanosine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D015122:Mercaptopurine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism* / Q000627:therapeutic use; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000188:drug therapy; D012680:Sensitivity and Specificity; D013866:Thioguanine / Q000032:analysis; D013872:Thionucleosides / Q000032:analysis; D014970:Xanthines / Q000032:analysis
D013872:Thionucleosides; D014970:Xanthines; D006151:Guanosine; C054700:6-thioxanthine; C010240:6-methylthiopurine; C005783:6-thioguanosine; D015122:Mercaptopurine; D013866:Thioguanine