Central administration of phosphatidylserine attenuates isolation stress-induced behavior in chicks.
The present study investigated whether centrally administered phosphatidylserine (PS) could modify the behavior of chicks under isolation-induced stress. Isolation stress-induced vocalization and spontaneous activity for 10 min, which were attenuated by intracerebroventricular (i.c.v.) injection of PS. The effect of PS was compared with other phospholipids or L-serine, a constituent of PS. Phosphatidylcholine (PC) had no effect on these behavior, but phosphatidylethanolamine (PE) significantly increased vocalizations and spontaneous activity compared with PS. L-Serine similarly decreased isolation-induced vocalizations and spontaneous activity. To clarify the mechanism by which central PS attenuates isolation-induced stress behavior, the contribution of the acetylcholine (ACh) receptor (AChR) was also investigated. PS was co-injected i.c.v. with the muscarinic AChR (M-AChR) antagonist scopolamine or the nicotinic AChR (N-AChR) antagonist hexamethonium. The suppression of vocalizations and spontaneous activity by PS was partially attenuated by scopolamine, but not hexamethonium. These findings indicate that isolation-induced stress behavior are attenuated by PS, acting partially through the M-AChR.
Koutoku
Tomoyuki
T
Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Takahashi
Hirokazu
H
Tomonaga
Shozo
S
Oikawa
Daichi
D
Saito
Shin
S
Tachibana
Tetsuya
T
Han
Li
L
Hayamizu
Kohsuke
K
Denbow
D Michael
DM
Furuse
Mitsuhiro
M
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D007276:Injections, Intraventricular; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D018733:Nicotinic Antagonists / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000008:administration & dosage / Q000378:metabolism; D010718:Phosphatidylserines / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D012694:Serine / Q000008:administration & dosage / Q000378:metabolism; D012934:Social Isolation; D013315:Stress, Psychological / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014828:Vocalization, Animal / Q000187:drug effects / Q000502:physiology
D018727:Muscarinic Antagonists; D018733:Nicotinic Antagonists; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D011976:Receptors, Muscarinic; C483858:phosphatidylethanolamine; D012694:Serine