title: Expression and changes of Ca2+-ATPase in neurons and astrocytes in the gerbil hippocampus after transient forebrain ischemia.
abstract: Ca2+-ATPase is one of the most powerful modulators of intracellular calcium levels. In this study, we focused on chronological changes in the immunoreactivity and protein levels of Ca2+-ATPase in the hippocampus after 5 min of transient forebrain ischemia. Ca2+-ATPase immunoreactivity was significantly altered in the hippocampal CA1 region and in the dentate gyrus, but not in the CA2/3 region after ischemic insult. In the sham-operated group, Ca2+-ATPase immunoreactivity was detected in the hippocampus. Ca2+-ATPase immunoreactivity in the CA1 region and in the dentate gyrus, and its protein levels peaked 3 h after ischemic insult. At this time, CA1 pyramidal cells and dentate polymorphic cells showed strong Ca2+-ATPase immunoreactivity. Thereafter, Ca2+-ATPase immunoreactivity reduced in the CA1 region and in the dentate gyrus. One day after ischemic insult, Ca2+-ATPase immunoreactivity was observed in some CA1 non-pyramidal cells, and 4 days after ischemic insult, Ca2+-ATPase immunoreactivity was detected in astrocytes throughout the CA1 region, but Ca2+-ATPase immunoreactivity in the dentate gyrus had nearly disappeared. Our results suggest that Ca2+-ATPase changes may be associated with a response to ischemic damage in hippocampal CA1 pyramidal cells, and that increased Ca2+-ATPase immunoreactivity in the reactive astrocytes may be associated with the maintenance of intracellular calcium levels.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
authors.forename: Hyoseon
authors.initials: H
authors.lastname: Shin
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Song
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Byoung Hee
authors.initials: BH
authors.lastname: Han
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D016923:Cell Death; D005849:Gerbillinae; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D009474:Neurons / Q000201:enzymology; D016548:Prosencephalon / Q000098:blood supply* / Q000201:enzymology; D017966:Pyramidal Cells / Q000166:cytology / Q000201:enzymology*; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000252:Calcium-Transporting ATPases