A new reversion assay for measuring all possible base pair substitutions in Saccharomyces cerevisiae.
A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.
Williams
Teresa-Marie
TM
Department of Biology, Emory University, Atlanta, Georgia 30322, USA.
Fabbri
Rebecca M
RM
Reeves
Jason W
JW
Crouse
Gray F
GF
D000595:Amino Acid Sequence; D001483:Base Sequence / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment