Region-specific changes of calcium/calmodulin-dependent protein kinase IV in the mouse brain following chronic morphine treatment.
In this study, we examined changes in expression of calcium/calmodulin-dependent protein kinase IV (CaMKIV) in the mouse brain following chronic morphine treatment. Double immunohistochemical staining showed strong colocalization of CaMKIV with mu-opioid receptors. Chronic treatment with morphine produced an increase in expression of CaMKIV and phosphorylated cAMP response element-binding protein (pCREB) in the CA3 region of the hippocampus, whereas a decrease in CaMKIV and pCREB expression was observed in the caudate putamen. Interestingly, chronic morphine induced a decrease in protein expression of CaMKIV in the basolateral amygdale and the primary somatosensory cortex without any concomitant changes in pCREB. These findings suggest that CaMKIV-dependent signaling may play a role in chronic morphine-induced neuroplasticity in a brain region-specific manner.
Nemmani
Kumar V S
KV
Department of Psychology and Centre for Research on Pain, McGill University, 1205 Dr Penfield Avenue, Montréal, Canada, Que. H3A 1B1.
Lalonde
Jasmin
J
Mogil
Jeffrey S
JS
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles / Q000378:metabolism; D008297:Male; D051379:Mice; D009020:Morphine / Q000008:administration & dosage*; D009294:Narcotics / Q000008:administration & dosage*; D010766:Phosphorylation; D017450:Receptors, Opioid, mu / Q000378:metabolism
D017362:Cyclic AMP Response Element-Binding Protein; D007211:Indoles; D009294:Narcotics; D017450:Receptors, Opioid, mu; C007293:DAPI; D009020:Morphine; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517598:Camk1 protein, mouse; C517685:Camk4 protein, mouse; C517681:Pnck protein, mouse