title: Cultivation system using glass beads immersed in liquid medium facilitates studies of Streptomyces differentiation.
abstract: A two-phase cultivation system was developed which will enable studies of streptomycete differentiation by molecular biological and global techniques such as transcriptomics and proteomics. The system is based on a solid phase formed by glass beads corresponding to particles in soil, clay, or sand natural habitats of streptomycetes. The beads are immersed in a liquid medium that allows easy modification or replacement of nutrients and growth factors as well as radioactive labeling of proteins. Scanning electron microscopy was used to analyze morphological differentiation of streptomycetes on glass beads and two-dimensional protein electrophoresis to demonstrate the potential of the system for analyses of protein synthesis profiles during the developmental program. This system facilitates studies of differentiation including expression and post-translation modifications of streptomycetes proteins, secondary metabolite biosynthesis, and morphological development.
authors.affiliation: Institute of Microbiology, Czech Academy of Sciences, 14220 Prague 4, Czech Republic.
authors.forename: Liem D
authors.initials: LD
authors.lastname: Nguyen
authors.forename: Ladislava
authors.initials: L
authors.lastname: Kalachová
authors.forename: Jana
authors.initials: J
authors.lastname: Novotná
authors.forename: Martin
authors.initials: M
authors.lastname: Holub
authors.forename: Olga
authors.initials: O
authors.lastname: Kofronová
authors.forename: Oldrich
authors.initials: O
authors.lastname: Benada
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Thompson
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Weiser
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D005898:Glass; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D040901:Proteomics; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media