Oxidation of glucose to gluconic acid by glucose oxidase in a membrane bioreactor.
Glucose oxidase (GO) (EC 1.1.3.4) was used as catalyst for oxidizing glucose into gluconic acid utilizing a 10-mL Bioengineering Enzyme Membrane Reactor or a 400-mL Millipore Stirred Ultrafiltration Cell (MSUC) coupled with a Millipore UF membrane (cutoff of 100 kDa) and operated for 12 h under an agitation of 100 rpm, pH 5.5, and 30 degrees C. The effect of feeding rate (0.10, 0.15, or 0.20 min-1), glucose (2.5 or 5.0 mM), and GO (1.0 or 2.0 mg/mL) concentrations on the catalysis were studied. A yield of about 75% was attained when the MSUC filled with 1.0 mg/mL of GO was fed with 2.5 mM glucose solution at a rate of 0.15 min-1.
Tomotani
Ester Junko
EJ
University of São Paulo, School of Pharmaceutical Sciences, Department of Biochemical and Pharmaceutical Technology, Av. Prof. Lineu Prestes, 580, B-16, 05508-900, São Paulo, SP, Brazil.
das Neves
Luiz Carlos Martins
LC
Vitolo
Michele
M
D019149:Bioreactors; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005942:Gluconates / Q000138:chemical synthesis*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods*
D004800:Enzymes, Immobilized; D005942:Gluconates; D008567:Membranes, Artificial; D005949:Glucose Oxidase; D005947:Glucose; C030691:gluconic acid