title: Bio-artificial periosteum for severe open fracture--an experimental study of osteogenic cell/collagen sponge composite as a bio-artificial periosteum.
abstract: In an attempt to reduce complications in cases of severe open fracture, we developed a bio-artificial periosteum composed of osteogenic cells and collagen sponge. In the present study, we evaluated the osteogenic potential of the bio-artificial periosteum in vivo and in vitro. After 4-week incubation in vitro, the bio-artificial periosteum had high alkaline phosphatase activity and osteocalcin content. Moreover, energy dispersive X-ray analysis revealed numerous crystal structures consisting of P and Ca on the surface of the bio-artificial periosteum. Using a rat model for severe bone injury, we examined the bone formation process in defect sites covered with the bio-artificial periosteum. New bone formation occurred in the central part of the bone defect as well as at the bone edge. We conclude that by using the bio-artificial periosteum, the fracture site benefited from an improved osteogenic environment. These results indicate that a clinical trial to further evaluate this technique should be conducted.
authors.affiliation: Department of Orthopaedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. hattori@naramed-u.ac.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Hattori
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takakura
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Aoki
authors.forename: Masato
authors.initials: M
authors.lastname: Sonobe
authors.forename: Naohide
authors.initials: N
authors.lastname: Tomita
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D021522:Bioartificial Organs; D001672:Biocompatible Materials / Q000737:chemistry*; D001698:Biomedical Engineering / Q000379:methods*; D001854:Bone Marrow Cells / Q000166:cytology; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000648:ultrastructure; D003094:Collagen / Q000737:chemistry*; D017102:Fracture Healing; D005597:Fractures, Open / Q000628:therapy*; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000737:chemistry / Q000378:metabolism; D010012:Osteogenesis; D010521:Periosteum / Q000737:chemistry* / Q000000981:diagnostic imaging; D011859:Radiography; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase