Gill reactivity of aluminium-species following liming.
In acidified aluminium (Al) rich freshwater positively charged Al-species (Al(i)) are the key toxic components due to the accumulation in fish gills. As a countermeasure, liming is used to increase the pH and reduce the concentration of Al(i)-species; in particular low molecular mass (LMM) Al(i)-species by hydrolyses. However, very toxic high molecular mass (HMM) Al polymers can form in the unstable mixing zone immediately after liming. In the present work gill reactivity of LMM and HMM Al-species was studied under controlled conditions in eight channel-tank mixing zone systems in the field where Atlantic salmon (Salmo salar L.) kept in cages were exposed to defined mixing zone water. Mixing zones were created by continually liming acid river water (pH 5.0-5.7) high in LMM Al(i) to pH 6.0 and 6.4, respectively. Transformation processes affecting the Al-speciation as a function of time after liming were documented by in situ hollow fibre ultrafiltration interfaced with ion chromatography, while the Al accumulation in fish gills was used as bioindicator. For fish exposed to mixing zone water immediately after liming (1 min) the Al accumulation in gills (mug Al g(-1)) was higher (factor of 2) than for fish exposed to acid water prior to liming, due to the formation of gill reactive HMM Al(i)-species. The Al accumulation in gills followed a first order kinetic expression reaching steady-state conditions after 24-h exposures. The deposition rate of Al in gills (mug Al g(-1) h(-1)) correlated with the water concentrations of HMM Al(i) (R(2)=0.80) in the mixing zones, and for LMM Al(i) in the acid water (R(2)=0.92). Due to the transient nature of HMM Al(i) the deposition rate of Al decreased from the point of liming with a factor of 10 downstream the channel-tank system (i.e. 100 min after liming). The concentration of gill accumulated Al was higher (factor of 3) immediately after high level liming (pH 6.4) than following low level liming (pH 6.0). However, high level liming was more efficient in detoxifying Al in downstream waters. Furthermore, the bioavailability of a given LMM Al(i) concentration as well as bioreactivity following liming was dependent on the TOC and silicon concentration in the acid water. Increased concentration of TOC (1.5 to 4.3 mg l(-1)) and silicon (0.3 to 1.0 mg l(-1)) reduced the gill reactivity of Al(i) by approximately 50%.
Teien
Hans-Christian
HC
Isotope Laboratory, Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway. hans-christian.teien@umb.no
Kroglund
Frode
F
Salbu
Brit
B
Rosseland
Bjørn Olav
BO
D000535:Aluminum / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D000818:Animals; D001681:Biological Assay; D001682:Biological Availability; D017610:Calcium Compounds / Q000737:chemistry; D005880:Gills / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D010087:Oxides / Q000737:chemistry; D011108:Polymers; D020317:Salmo salar / Q000502:physiology*; D014873:Water Pollutants / Q000493:pharmacokinetics* / Q000633:toxicity
D017610:Calcium Compounds; D010087:Oxides; D011108:Polymers; D014873:Water Pollutants; C016538:lime; D000535:Aluminum