A novel molecular marker of pituitary tumor transforming gene involves in a rat liver regeneration.
BACKGROUND
Pituitary tumor transforming gene (PTTG), homologous to a mammalian securin, plays a pivotal role in cell transformation, however, its biological function(s) in normal tissues is not fully understood. Because the liver is a regenerative organ, the relevant biological function of PTTG in the liver would be more feasible to understand PTTG. Also, PTTG may be involved in the liver regeneration.


METHODS
Expressions of rat hepatic PTTG messengerRNA (mRNA) and cellular immunoreactivities during the cell proliferative period of the liver regeneration both in vitro and in vivo were tested.


RESULTS
PTTG expression of the rat primary hepatocyte was stimulated by HGF in a dose dependent manner, and was suppressed when hepatocyte proliferation was inhibited by transforming growth factor-beta1. A positive PTTG immunoreactive co-localizing with 5-bromo-2'-deoxyuridine (BrdU) in the hepatocyte nucleus was found and there was a concurrent sister chromatin itself by the immunofluorescent labeling of PTTG with cytokeratin 18 (CK18).


CONCLUSIONS
Since the correlation of PTTG mRNA expression, cell proliferation and immunoreactivity were observed in primary rat cultured hepatocytes, PTTG may be a novel marker of cell proliferation both in vitro and in vivo liver regeneration.
Akino
Kozo
K
Department of Medicine, Cedars-Sinai Research Institute UCLA School of Medicine, Los Angeles, California, USA.
Akita
Sadanori
S
Mizuguchi
Toru
T
Takumi
Ichiro
I
Yu
Run
R
Wang
Xhi-yong
XY
Rozga
Jacek
J
Demetriou
Achilles A
AA
Melmed
Shlomo
S
Ohtsuru
Akira
A
Yamashita
Shunichi
S
D000818:Animals; D015415:Biomarkers; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D015870:Gene Expression / Q000187:drug effects; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008115:Liver Regeneration / Q000235:genetics*; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D064249:Securin; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
D015415:Biomarkers; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D064249:Securin; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor; D007633:Keratins; D001973:Bromodeoxyuridine