Quality assessment of microarray experiments.
OBJECTIVE
We describe a quality assurance procedure to maximize the value of oligonucleotide microarray expression profiles.


METHODS
Background microarray noise was 82.2+/-54.5 and 51.8+/-12.4 units, respectively, before and after enacting the program (P<0.0001). We also noted improved concordance of microarray expression fold-changes for selected genes with results of RT-PCR validation.


CONCLUSIONS
This multi-step procedure, including quantification of RNA sample degradation and detection of outlier data points, has increased data quality from our microarray facility.
Carter
David E
DE
London Regional Genomics Centre, Robarts Research Institute, London, Ontario, Canada N6A 5K8.
Robinson
John F
JF
Allister
Emma M
EM
Huff
Murray W
MW
Hegele
Robert A
RA
D000818:Animals; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D011786:Quality Control; D012313:RNA / Q000592:standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software
D012313:RNA