title: Preparation of porcine small intestinal submucosa sponge and their application as a wound dressing in full-thickness skin defect of rat.
abstract: Small intestinal submucosa (SIS) sponge was prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The prepared SIS sponges exhibited elastic and soft property on touch and were ease to handle. The SIS sponges have the pore diameter of 100-200 microm and an interconnective porous structure. The SIS sponges exhibited high water absorption ability over 8000%. The water uptake of SIS sponges decreased as SIS concentration used to manufacture SIS sponge increased. In wound healing test, SIS sponge attained uniform adherence to the wound surface. The SIS sponges absorbed higher extent of exudation for wound than that covered with Tegaderm as control. Wound area contracted above 80% at the 21st postoperative day. The SIS sponge treated wound was almost completely covered with a thin layer of epidermis at 4 weeks. In addition, the dermal collagen in the wound regenerated at only SIS sponges treated wounds. The progress of granulous tissue formation was faster in SIS sponges as wound dressing than in Tegaderm. In conclusion, we found that the SIS sponges might be a potential material as a wound dressing.
authors.affiliation: Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Republic of Korea.
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Keum Duck
authors.initials: KD
authors.lastname: Hong
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Shin
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Kim
authors.forename: Soon Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Min Suk
authors.initials: MS
authors.lastname: Lee
authors.forename: Woo Young
authors.initials: WY
authors.lastname: Jang
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
authors.forename: Hai Bang
authors.initials: HB
authors.lastname: Lee
mesh_terms: D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D001684:Biological Dressings; D001706:Biopsy; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D007249:Inflammation; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011161:Porifera; D011208:Powders / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones / Q000737:chemistry; D012867:Skin / Q000378:metabolism; D017768:Skin, Artificial; D013552:Swine; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011208:Powders; D012828:Silicones; D014867:Water; D005022:Ethyldimethylaminopropyl Carbodiimide