title: Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle.
abstract: The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyperoxia on UCP3 expression in mouse skeletal muscle and C2C12 myotubes. We found that the hyperoxia-mediated oxidative stress was associated with a 5-fold and 3-fold increase of UCP3 mRNA and protein levels, respectively, in mouse muscle. Hyperoxia also enhanced reactive oxygen species production and UCP3 mRNA expression in C2C12 myotubes. Our findings support the view that both in vivo and in vitro UCP3 may modulate reactive oxygen species production in response to an oxidative stress.
authors.affiliation: Department of Cell Physiology and Metabolism, Centre Médical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland. pflandin@itsa.ucsf.edu
authors.forename: Pierre
authors.initials: P
authors.lastname: Flandin
authors.forename: Yves
authors.initials: Y
authors.lastname: Donati
authors.forename: Constance
authors.initials: C
authors.lastname: Barazzone-Argiroffo
authors.forename: Patrick
authors.initials: P
authors.lastname: Muzzin
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D007473:Ion Channels; D051379:Mice; D024101:Mitochondrial Proteins; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000071247:Uncoupling Protein 3
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3