Enzymatic synthesis of a novel cyclic pentasaccharide consisting of alpha-D-glucopyranose with 6-alpha-glucosyltransferase and 3-alpha-isomaltosyltransferase.
A novel cyclic pentasaccharide (CPS) and a branched cyclic pentasaccharide (6G-CPS) consisting of d-glucopyranose were synthesized with 6-alpha-glucosyltransferase (6GT) and 3-alpha-isomaltosyltransferase (IMT) from Bacillus globisporus N75. The structure of CPS was cyclo-[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->]. The other, 6G-CPS, had the structure cyclo-[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-[alpha-D-Glcp-(1-->6)]-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->]. The formation of CPS was presumed to occur after the following four successive reactions: a 6-glucosyltransfer reaction with 6GT, a 4-glucosyltransfer reaction with 6GT, a 3-isomaltosyltransfer reaction with IMT, and a cyclization reaction with IMT.
Watanabe
Hikaru
H
Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase minami-machi, Okayama 700-0834, Japan. h-watanabe@hayashibara.co.jp
Nishimoto
Tomoyuki
T
Aga
Hajime
H
Kubota
Michio
M
Fukuda
Shigeharu
S
Kurimoto
Masashi
M
Tsujisaka
Yoshio
Y
D001407:Bacillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism*; D047028:Macrocyclic Compounds / Q000737:chemistry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013213:Starch / Q000737:chemistry
D047028:Macrocyclic Compounds; D009844:Oligosaccharides; C504006:cyclo(-6)-glucpyranosyl-1-3-glucopyranosyl-1-6-glucopyranosyl-1-3-(glucopyranosyl-1-6)-glucopyranosyl-1-4-glucopyranosyl-(1-); C504005:cyclo(-6)-glucpyranosyl-1-3-glucopyranosyl-1-6-glucopyranosyl-1-3-glucopyranosyl-1-4-glucopyranosyl-(1-); D013213:Starch; D005964:Glucosyltransferases; C436710:maltosyltransferase; D005947:Glucose