title: Transcriptional response of Enterococcus faecalis V583 to erythromycin.
abstract: A transcriptional profile of Enterococcus faecalis V583 (V583) treated with erythromycin is presented. This is the first study describing a complete transcriptional profile of Enterococcus. E. faecalis is a common and nonvirulent bacterium in many natural environments, but also an important cause of nosocomial infections. We have used a genome-wide microarray based on the genome sequence of V583 to study gene expression in cells exposed to erythromycin. V583 is resistant to relatively high concentrations of erythromycin, but growth is retarded by the treatment. The effect of erythromycin treatment on V583 was studied by a time course experiment; samples were extracted at five time points over a period of 90 min. A drastic change in gene transcription was seen with the erythromycin-treated cells compared to the untreated cells. Altogether, 260 genes were down-regulated at one or more time points, while 340 genes were up-regulated. Genes encoding hypothetical proteins and genes encoding transport and binding proteins were the two most dominating groups of differentially expressed genes. The gene encoding ermB (EFA0007) was expressed, but not differentially, which indicated that other genes are important for the survival and growth maintenance of V583 treated with erythromycin. One of these genes is a putative MsrC-like protein, which was up-regulated at all time points studied. Other specific genes that were found to be up-regulated were genes encoding ABC transporters and two-component regulatory systems, and these may be genes that are important for the specific response of V583 to erythromycin.
authors.affiliation: Laboratory of Microbial Gene Technology, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway. agot.aakra@umb.no
authors.forename: Agot
authors.initials: A
authors.lastname: Aakra
authors.forename: Heidi
authors.initials: H
authors.lastname: Vebø
authors.forename: Lars
authors.initials: L
authors.lastname: Snipen
authors.forename: Helmut
authors.initials: H
authors.lastname: Hirt
authors.forename: Are
authors.initials: A
authors.lastname: Aastveit
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
authors.forename: Gary
authors.initials: G
authors.lastname: Dunny
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
authors.forename: Barbara
authors.initials: B
authors.lastname: Murray
authors.forename: Ingolf F
authors.initials: IF
authors.lastname: Nes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D004917:Erythromycin / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004917:Erythromycin