title: Human embryonic stem cell lines derived from the Chinese population.
abstract: Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.
authors.affiliation: Center for Developmental Biology, Xinhua Hospital, Shanghai Second Medical University, Shanghai, China.
authors.forename: Zhen Fu
authors.initials: ZF
authors.lastname: Fang
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Gai
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Ai Lian
authors.initials: AL
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Hui Zhen
authors.initials: HZ
authors.lastname: Sheng
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D002681:China; D019976:Cloning, Organism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D053652:Nuclear Transfer Techniques; D044382:Population Groups; D013724:Teratoma / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D006680:HLA Antigens