Generation of mouse polyclonal and human monoclonal antibodies against Bacillus anthracis toxin.
High titer antisera against the protective antigen (PA) from Bacillus anthracis were generated immunizing Balb/c mice two times intraperitoneally with PA in combination with lipopeptide adjuvant P3CSK4. The sera were able to protect the mouse macrophage cell line J774A.1 from an anthrax toxin challenge. We also tested the blood of anthrax vaccine-immunized persons for PA- and lethal factor (LF)-specific antibodies. An increased titer was found after three immunizations, and the sera were also able to protect the mouse macrophage cell line from a toxin challenge. For the preparation of human monoclonal antibodies, we used peripheral blood lymphocytes. After in vitro stimulation using PA or synthetic peptides derived from PA, B lymphocytes were immortalized by PEG fusion with the human mouse heteromyeloma cell line CB-F7. We obtained several clones producing high amounts of PA-specific immunoglobulin (Ig).
Huber
M
M
Institute of Molecular Medicine and Cell Research, University Hospital, Freiburg, Germany. maria.huber@mol-med.uni-freiburg.de
Vor Dem Esche
U
U
Grunow
R
R
Bessler
W G
WG
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology*; D000992:Antitoxins / Q000096:biosynthesis*; D001408:Bacillus anthracis / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C030325:anthrax toxin; D005620:Freund's Adjuvant