Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea.
From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in 2002 formed one distinct cluster, which different from clusters of Korean isolates in 2000, with in the same lineage of the ME-SA topotype strains. Deduced amino acid sequences around neutralizable antigenic site on VP1 site of O/SKR/2002 isolates were aligned and compared with other strains. At the antigenic site 1, the replacements of the critical amino acid residues at position 144 from V to L and at position 152 from A to T were observed in O/SKR/2002 viruses. For antigenic site 2 and 4, there were not significant variations in general. At the antigenic site 3, the substitutions of amino acid residues were present at positions 54 and 56 in O/SKR/2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O/SKR/AS/2002 (cow) virus. And the substitution (L-->P) of significant residue at position 144 was detected at the amino acid sequence of the O/SKR/2002 (cow) virus.
Oem
Jae Ku
JK
National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Korea. jku0622@nvrqs.go.kr
Lee
Kwang Nyeong
KN
Cho
In Soo
IS
Kye
Soo Jeong
SJ
Park
Jee Yong
JY
Park
Jong Hyeon
JH
Kim
Yong Joo
YJ
Joo
Yi Seok
YS
Song
Hee Jong
HJ
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D016000:Cluster Analysis; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000032:analysis; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D012367:RNA, Viral; C442501:VP1 protein, Foot-and-mouth disease virus