Serum TGF-beta 1 and TNF-alpha levels and cardiac fibrosis in experimental chronic renal failure.
OBJECTIVE
To characterize dynamics of changes of serum levels of TGF-beta1 and TNF-alpha in rats with cardiac fibrosis (CF) occurring during chronic renal failure (CRF), and to reveal the character pf correlations of these factors with amounts of cardiac collagen.


METHODS
CRF was induced by unilateral nephrectomy and by electrocoagulation of 25% of the cortex of remnant kidney. Post-operative checkpoints were 2, 4, and 6 months. Serum TGF-beta1 and TNF-alpha levels were measured by ELISA.


RESULTS
CF became pronounced only at 6 months of CRF, while serum TGF-beta1 concentrations reached maximum at 4 months, i.e., at the checkpoint preceding the development of CF. Multiple regression showed cardiac collagen to correlate with both serum TGF-beta1 levels and time from the onset of CRF. Sensitivity and specificity of TGF-beta1 as serum marker of CF were 86% and 75%.
Fedulov
Alexey V
AV
St. Petersburg State Pavlov Medical University, Russia. afedulov@hsph.harvard.edu
Ses
T P
TP
Gavrisheva
N A
NA
Rybakova
M G
MG
Vassilyeva
J G
JG
Tkachenko
S B
SB
Kallner
A
A
MacMillan
J C
JC
D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005355:Fibrosis / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000473:pathology*; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D012680:Sensitivity and Specificity; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000097:blood*; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D015415:Biomarkers; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha