title: Comparative evaluation of the VIDAS HIV DUO Ultra assay for combined detection of HIV-1 antigen and antibodies to HIV.
abstract: The aim of the study was to evaluate the performance of the combined antigen and antibody HIV screening assay VIDAS HIV DUO Ultra (BioMérieux, Marcy l'Etoile, France) in comparison with two other combined tests: the former version of the same test (VIDAS HIV DUO, BioMérieux) and the AxSYM HIV Ag/Ab Combo assay (Abbott Laboratories, Rungis, France). A prospective study was performed on serum specimens received on a routine basis for HIV testing: 1443 blood samples were tested with the three assays. Sensitivity was 100% for the three tests. Specificity assessed on repeated false-positive samples was 99.86, 99.03 and 99.65% for VIDAS HIV DUO Ultra, VIDAS HIV DUO and AxSYM HIV Ag/Ab Combo, respectively. In addition, 14 seroconversion panels were tested with the VIDAS DUO Ultra and AxSYM HIV Ag/Ab Combo assays. For four of these panels, a positive signal was detected one blood sampling point earlier with the VIDAS DUO Ultra assay, corresponding to a higher sensitivity of the HIV antigen test. These results indicate that the VIDAS HIV DUO Ultra exhibits an improved specificity with comparison to the former version of this assay and an excellent sensitivity for early detection of HIV seroconversion.
authors.affiliation: Laboratoire de Bactériologie-Virologie, Hôpital Nord, CHU de Saint-Etienne, 42055 Saint-Etienne Cedex 02, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bourlet
authors.forename: Catherine
authors.initials: C
authors.lastname: Pretis
authors.forename: Sylvie
authors.initials: S
authors.lastname: Pillet
authors.forename: Mylène
authors.initials: M
authors.lastname: Lesenechal
authors.forename: Jérôme
authors.initials: J
authors.lastname: Piche
authors.forename: Bruno
authors.initials: B
authors.lastname: Pozzetto
mesh_terms: D015492:AIDS Serodiagnosis; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D011933:Reagent Kits, Diagnostic