title: [Discovery of a new splicing type of KCHIP1 gene].
abstract: The present study aimed to find the mutations of KCHIP1 gene in breast cancer. KCHIP1 cDNA samples from 12 specimens of breast cancer and 12 specimens of normal mammary tissues were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and directly sequenced to detect mutation. No mutation of KCHIP1 gene was found in these samples; while a new splicing type of KCHIP1 gene was found, which has an insert (162 bp) between exon 1 and exon 2 in KCHIP1 gene (AY780424).
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, P. R. China. liuzheng19752002@yahoo.com.cn
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Xiao
authors.forename: Fei-Yue
authors.initials: FY
authors.lastname: Fan
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Sun
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Li
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Yang
mesh_terms: D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D006801:Humans; D051605:Kv Channel-Interacting Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; C496750:KCNIP1 protein, human; D051605:Kv Channel-Interacting Proteins