Use of the zirconia-based stationary phase for separation of ibuprofen and its impurities.
A new reversed-phase liquid chromatographic method using zirconia-based stationary phase was developed for determination of ibuprofen, its related compounds and its main degradation products. The chromatographic separation was successfully achieved on the Discovery Zr-PS column (150 mm x 4.6 mm i.d., 5 microm), using a mobile phase methanol-phosphate buffer (pH 4.5; 0.05 M)-tetrahydrofurane (21:74:5, v/v/v) and the flow rate 0.5 ml min(-1). The UV detection was performed in dual wavelength mode (219 and 258 nm) to detect all compounds of interest. The column temperature was set on 60 degrees C to shorten the analysis time and improve the peak symmetry. The method is simple, rapid and cuts down the amount of hazardous waste produced in the analysis. The assay is completed within 22 minutes.
Kucera
R
R
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, CZ-50005 Hradec Králové, Czech Republic. kucerar@faf.cuni.cz
Sochor
J
J
Klimes
J
J
Dohnal
J
J
D002021:Buffers; D004340:Drug Contamination; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000302:isolation & purification*; D011137:Polystyrenes / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D015040:Zirconium / Q000737:chemistry*
D002021:Buffers; D011137:Polystyrenes; D015040:Zirconium; C028541:zirconium oxide; D007052:Ibuprofen