[Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo].
OBJECTIVE
To investigate the effect of IL-24 expression on the growth of glioma cells.


METHODS
The IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor.


RESULTS
It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.


CONCLUSIONS
IL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.
Li
Wen-ling
WL
Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.
Shan
Bao-en
BE
Yan
Yun-li
YL
Zheng
Li-fen
LF
Li
Qiao-xia
QX
Zhou
Na-jing
NJ
Zhao
Wen-qing
WQ
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D000099180:Interleukin-24
D007378:Interleukins; D011994:Recombinant Proteins; D000099180:Interleukin-24