title: Fate of prions in soil: trapped conformation of full-length ovine prion protein induced by adsorption on clays.
abstract: Studying the mechanism of retention of ovine prion protein in soils will tackle the environmental aspect of potential dissemination of scrapie infectious agent. We consider the surface-induced conformational changes that the recombinant ovine prion protein (ovPrP) may undergo under different pH conditions when interacting with soil minerals of highly adsorptive capacities such as montmorillonite. The conformational states of the full-length ovine prion protein adsorbed on the electronegative clay surface are compared to its solvated state in deuterated buffer in the pD range 3.5-9, using FTIR spectroscopy. The in vitro pH-induced conversion of the alpha-helical monomer of ovPrP into oligomers of beta-like structure prone to self-aggregation does not occur when the protein is adsorbed on the clay surface. The conformation of the trapped ovPrP molecules on montmorillonite is pH-independent and looks like that of the ovPrP solvated state at pD higher than 7, suggesting the major role of Arg and Lys residues in the electrostatic origin of adsorption. The uneven distribution of positively and negatively charged residues of the ovPrP protein would promote a favored orientation of the protein towards the clay, so that not only the basic residues embedded in the N-terminal flexible part but also external basic residues in the globular part of the protein might participate to the attractive interaction. From these results, it appears unlikely that the interaction of normal prions (PrP(C)) with soil clay surfaces could induce a change of conformation leading to the pathogenic form of prions (PrP(Sc)).
authors.affiliation: Laboratoire de Dynamique, Interactions et Réactivité CNRS-Université Pierre et Marie Curie, UMR 7075, 2 rue Henri Dunant, 94320 Thiais, France.
authors.forename: M
authors.initials: M
authors.lastname: Revault
authors.forename: H
authors.initials: H
authors.lastname: Quiquampoix
authors.forename: M H
authors.initials: MH
authors.lastname: Baron
authors.forename: S
authors.initials: S
authors.lastname: Noinville
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000493:pharmacokinetics*; D000818:Animals; D001546:Bentonite / Q000493:pharmacokinetics; D000077215:Clay; D006863:Hydrogen-Ion Concentration; D011328:Prions / Q000737:chemistry* / Q000493:pharmacokinetics; D011487:Protein Conformation; D012756:Sheep; D012987:Soil; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000538:Aluminum Silicates; D011328:Prions; D012987:Soil; D012996:Solutions; D001546:Bentonite; D000077215:Clay