title: [High expression and characterization of human parathyroid hormone in Escherichia coli].
abstract: Human parathyroid hormone (hPTH) was highly expressed in Escherichia coli by inserted the synthesized whole hPTH cDNA into the vectors pBV220 and pET22b. After expression and disruption, the purified product was acquired through cation exchange chromatography and reverse phase chromatography. From the results of N-terminal sequencing and MALDI-TOF-MS analysis the recombiant prtein was indentified as intact hPTH. In in vitro Bioassays the recombinant hPTH stimulated adenylate cyclase as the standard did. In ovariectomized rats the recombinant hPTH markedly increased the femoral bone mass and bone mineral density.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China. fanghongqing@hotmail.com
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Fang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Dai
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Li
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Zhao
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Deng
authors.forename: Chong
authors.initials: C
authors.lastname: Xue
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Liu
authors.forename: Hou-Chu
authors.initials: HC
authors.lastname: Zhu
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Ma
authors.forename: Hui-Peng
authors.initials: HP
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015519:Bone Density / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010281:Parathyroid Hormone