title: Genome sequences and structures of two biologically distinct strains of Grapevine leafroll-associated virus 2 and sequence analysis.
abstract: Grapevine leafroll-associated virus 2 (GLRaV-2), a member of the genus Closterovirus within Closteroviridae, is implicated in several important diseases of grapevines including "leafroll", "graft-incompatibility", and "quick decline" worldwide. Several GLRaV-2 isolates have been detected from different grapevine genotypes. However, the genomes of these isolates were not sequenced or only partially sequenced. Consequently, the relationship of these viral isolates at the molecular level has not been determined. Here, we group the various GLRaV-2 isolates into four strains based on their coat protein gene sequences. We show that isolates "PN" (originated from Vitis vinifera cv. "Pinot noir"), "Sem" (from V. vinifera cv. "Semillon") and "94/970" (from V. vinifera cv. "Muscat of Alexandria") belong to the same strain, "93/955" (from hybrid "LN-33") and "H4" (from V. rupestris "St. George") each represents a distinct strain, while Grapevine rootstock stem lesion-associated virus.
authors.affiliation: Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, N1G 2W1, Ontario, Canada. bmeng@uoguelph.ca
authors.forename: Baozhong
authors.initials: B
authors.lastname: Meng
authors.forename: Caihong
authors.initials: C
authors.lastname: Li
authors.forename: Dariusz E
authors.initials: DE
authors.lastname: Goszczynski
authors.forename: Dennis
authors.initials: D
authors.lastname: Gonsalves
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D017790:Closterovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000821:virology*
chemical_list: D036022:Capsid Proteins