[Effective molecular form of alpha-tocopheryl succinate in inducing apoptosis of SGC-7901 cells].
OBJECTIVE
In order to explore the effective molecular form of alpha-tocopherol succinate (alpha-TOS) in inducing apoptosis of human gastric cancer SGC-7901 cell.


METHODS
Apoptosis was detected by DAPI staining and DNA ladder, prior to the treatment of cells with alpha-TOS (5 microg/ml, 10 microg/ml and 20 microg/ml) for 48h, alpha-tocopherol (alpha-TOH) (20 microg/ml) and ethanol 1 microl/ml as control groups. HPLC was also used to examine alpha-TOS and alpha-TOH in the cells as well as culture medium.


RESULTS
The observation of DAPI showed that the chromatin in karyon of SGC-7901 cells in the 10 microg/ml and 20 microg/ml groups was concentrated with karyopyknosis and karyorrhexis, which were not found in other groups. Typical DNA ladders were obvious in the cells post-administration of alpha-TOS at concentrations 10 microg/ml and 20 microg/ml. It was alpha-TOS not its hydrolyzed product alpha-TOH that was screened out by HPLC in cells and culture medium.


CONCLUSIONS
alpha-TOS was capable of inducting the apoptosis of SGC-7901 cells in its intact form and was independent of alpha-TOH.
Li
Hong-wei
HW
School of Public Health, Harbin Medical University, Harbin 150001, China.
Zhao
Lan
L
Wu
Kun
K
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology*; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D014810:Vitamin E; D024505:Tocopherols