Overlap of the gene encoding the novel poly(ADP-ribose) polymerase Parp10 with the plectin 1 gene and common use of exon sequences.
We have recently identified PARP10 as a novel functional poly(ADP-ribose) polymerase. The gene encoding PARP10 is conserved in vertebrates but no orthologs were found in lower organisms. In addition to the poly(ADP-ribose) polymerase domain, PARP10 possesses several additional sequence motifs, including an RNA recognition motif and two ubiquitin interaction motifs. We characterized the murine genomic locus of the Parp10 gene. We noticed that 3' Parp10 sequences overlapped with the plectin 1 gene in a head-to-tail arrangement. Detailed analyses revealed that the two most 3' Parp10 exons (exons 10 and 11) are also used for plectin 1. While these two exons code for part of the poly(ADP-ribose) polymerase domain in Parp10, they are noncoding for plectin 1 due to the lack of appropriate start codons. Furthermore our findings suggest that at least one of the plectin 1 promoters is located within intron 9 of the Parp10 gene.
Lesniewicz
Krzysztof
K
Abteilung Biochemie und Molekularbiologie, Institut für Biochemie, Klinikum der RWTH, Pauwelsstrasse 30, 52057 Aachen, Germany.
Lüscher-Firzlaff
Juliane
J
Poreba
Elzbieta
E
Fuchs
Peter
P
Walsemann
Gesa
G
Wiche
Gerhard
G
Lüscher
Bernhard
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D030561:Databases, Nucleic Acid; D005091:Exons / Q000235:genetics*; D005807:Genes, Overlapping / Q000235:genetics*; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D051190:Plectin / Q000235:genetics*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D023741:Takifugu; D014158:Transcription, Genetic / Q000235:genetics
C495333:Plec protein, mouse; D051190:Plectin; D011065:Poly(ADP-ribose) Polymerases