Specific molecular interactions by force spectroscopy: from single bonds to collective properties.
Interactions involving multiple bonds occur throughout biology, and have distinct properties that are fundamentally different from those present in single bond systems. We have developed a new method to analyse the AFM force measurements in order to extract relevant information and to characterise the interactions involving from single to multiple bonds. Our study reveals a surprising behaviour in the presence of multiple bonds with a high rebinding probability: the mean binding forces increase with decreasing pulling velocity. Such behaviour is different from the force dependence on the loading rate for single bond rupture or existing models for multiple bonds rupture.
Lévy
R
R
Biosciences Center, University of Liverpool, Crown street, Liverpool L69 7ZD, United Kingdom.
Maaloum
M
M
D055592:Biophysical Phenomena; D001703:Biophysics; D007093:Imidazoles / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*
D007093:Imidazoles; C029899:imidazole