[Effects of nitrogen supply on nutritional quality and antioxidative enzyme activities of spinach].
A hydroponic experiment was carried out to study the effects of nitrogen (N) supply on the nutritional quality and antioxidative enzyme activities of spinach. The results showed that when the N supply increased from 4 to 8 mmol x L(-1), the biomass of spinach and its leaf vitamin C (Vc) content increased significantly, but a further increase of the N supply induced an obvious decrease of Vc content. An increasing N supply increased the nitrate content in leaves. The soluble oxalate content in leaves decreased slightly with the N supply from 4 to 8 mmol x L(-1) N, but increased with a further increase of supplied N. Total oxalate content increased firstly, and then decreased with increasing N supply. SOD and POD activities increased with the N level up to 8 mmol x L(-1) N, but drastically decreased with increasing N levels. CAT activity decreased when N level elevated. MDA content decreased firstly and then increased with increasing N levels, while free proline content decreased with increasing N supply. All of these indicated that 8 mmol x L(-1) N was an appropriate N supply level for spinach to improve its biomass, nutritional quality, and resistance.
Zhang
Yingpeng
Y
College of Environment and Resource, Zhejiang University, Hangzhou 310029, China. zhangyingpeng@163.com
Lin
Xianyong
X
Zhang
Yongsong
Y
D001205:Ascorbic Acid / Q000032:analysis; D018533:Biomass; D005308:Fertilizers; D009584:Nitrogen; D010070:Oxalates / Q000032:analysis; D018724:Spinacia oleracea / Q000737:chemistry* / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000032:analysis
D005308:Fertilizers; D010070:Oxalates; D013482:Superoxide Dismutase; D009584:Nitrogen; D001205:Ascorbic Acid