Does lignin modification affect feeding preference or growth performance of insect herbivores in transgenic silver birch (Betula pendula Roth)?
Transgenic silver birch (Betula pendula Roth) lines were produced in order to modify lignin biosynthesis. These lines carry COMT (caffeate/5-hydroxyferulate O-methyltransferase) gene from Populus tremuloides driven by constitutive promoter 35S CaMV (cauliflower mosaic virus) or UbB1 (ubiquitin promoter from sunflower). The decreased syringyl/guaiacyl (S/G) ratio was found in stem and leaf lignin of 35S CaMV-PtCOMT transgenic silver birch lines when compared to non-transformed control or UbB1-PtCOMT lines. In controlled feeding experiments the leaves of transgenic birch lines as well as controls were fed to insect herbivores common in boreal environment, i.e., larvae of Aethalura punctulata, Cleora cinctaria and Trichopteryx carpinata (Lepidoptera: Geometridae) as well as the adults of birch leaf-feeding beetles Agelastica alni (Coleoptera: Chrysomelidae) and Phyllobius spp. (Coleoptera: Curculionidae). The feeding preferences of these herbivores differed in some cases among the tested birch lines, but these differences could not be directly associated to lignin modification. They could as well be explained by other characteristics of leaves, either natural or caused by transgene site effects. Growth performance of lepidopteran larvae fed on transgenic or control leaves did not differ significantly.
Tiimonen
Heidi
H
Finnish Forest Research Institute, Punkaharju Research Station, Finlandiantie 18, 58450, Punkaharju, Finland. heidi.tiimonen@metla.fi
Aronen
Tuija
T
Laakso
Tapio
T
Saranpää
Pekka
P
Chiang
Vincent
V
Ylioja
Tiina
T
Roininen
Heikki
H
Häggman
Hely
H
D000818:Animals; D029662:Betula / Q000235:genetics / Q000378:metabolism*; D001517:Coleoptera / Q000254:growth & development / Q000502:physiology; D005247:Feeding Behavior; D017343:Genes, Plant; D007313:Insecta / Q000254:growth & development* / Q000502:physiology*; D007915:Lepidoptera / Q000254:growth & development / Q000502:physiology; D008031:Lignin / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology; D030821:Plants, Genetically Modified / Q000378:metabolism
D008031:Lignin