[Effect of epigallocatechingallate on acute lung injury induced by oleic acid in mice].
OBJECTIVE
To investigate the effect of epigallocatechingallate (EGCG) on acute lung injury induced by oleic acid in mice and the possible mechanism.


METHODS
Acute lung injury was induced by oleic acid in mice. Light microscopy and electron microscopy were used to examine histological changes and lung index as well as wet to dry weight ratio was calculated. Serum TNF-a level was measured by enzyme linked immunosorbent assay (ELISA) and the phosphorylation of p38 MAPK was determined by Western blotting.


RESULTS
Pretreatment of EGCG significantly alleviated oleic acid induced lung injury accompanied by reduction of lung index and wet to dry weight ratio, decreased of TNF-a level in serum and inhibition of phosphorylation of p38 MAPK.


CONCLUSIONS
EGCG showed beneficial effect on acute lung injury induced by oleic acid in mice. The ultimate reduction of TNF-alpha in serum caused by inhibition of phosphorylated p38 MAPK is involved in the mechanism of action of EGCG.
Xu
Guang-lin
GL
Center for Drug Research and Development, College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Yao
Lin
L
Yu
Shu-qin
SQ
Bu
Dan
D
Wang
Yan-fei
YF
Gong
Zhu-nan
ZN
Zhang
Shuang-quan
SQ
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008168:Lung / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D019301:Oleic Acid; D010766:Phosphorylation / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D019301:Oleic Acid; D002392:Catechin; C045651:epigallocatechin gallate; D048051:p38 Mitogen-Activated Protein Kinases