title: The presence of Propionibacterium spp. in the vitreous fluid of uveitis patients with sarcoidosis.
abstract: OBJECTIVE
  An immunological reaction to a bacterial antigen, such as Mycobacterium tuberculosis or Propionibacterium spp., is suspected to be an initial mechanism in the disorder known as sarcoidosis. We investigated whether or not P. acnes, P. granulosum or M. tuberculosis are present in the vitreous fluid of eyes suffering from uveitis with sarcoidosis.
  
  
  METHODS
  Using polymerase chain reaction, we analysed the presence of P. acnes, P. granulosum and/or M. tuberculosis DNA in vitreous samples taken from six eyes with sarcoidosis and six control eyes.
  
  
  RESULTS
  Among the six uveitis eyes with sarcoidosis, we detected P. acnes DNA in two eyes, P. granulosum DNA in four eyes, and both P. acnes and P. granulosum DNA in one eye, but no Propionibacterium spp. in the control eyes. M. tuberculosis DNA was not present in any of the patient or control eyes.
  
  
  CONCLUSIONS
  This is the first report indicating the presence of Propionibacterium spp. and/or its DNA in the vitreous fluid of sarcoidic eyes with uveitis. This, therefore, supports the idea that Propionibacterium spp. are involved in the aetiology of uveitis in sarcoidosis.
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. tyasuhar@ophth.kpu-m.ac.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Yasuhara
authors.forename: Rei
authors.initials: R
authors.lastname: Tada
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tei
authors.forename: Chikako
authors.initials: C
authors.lastname: Mochida
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kamei
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004269:DNA, Bacterial / Q000032:analysis; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011425:Propionibacterium acnes / Q000235:genetics / Q000302:isolation & purification*; D012507:Sarcoidosis / Q000382:microbiology*; D015866:Uveitis, Posterior / Q000382:microbiology*; D014822:Vitreous Body / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial