title: Physicochemical characterization of poly(L-lactic acid) and poly(D,L-lactide-co-glycolide) nanoparticles with polyethylenimine as gene delivery carrier.
abstract: Polymer nanoparticles have been used as non-viral gene delivery systems and drug delivery systems. In this study, biodegradable poly(L-lactic acid) (PLA)/polyethylenimine (PEI) and poly(D,L-lactide-co-glycolide) (PLGA)/PEI nanoparticles were prepared and characterized as gene delivery systems. The PLA/PEI and PLGA/PEI nanoparticles, which were prepared by a diafiltration method, had spherical shapes and smooth surface characteristics. The size of nanoparticles was controlled by the amount of PEI, which acted as a hydrophilic moiety, which effectively reduced the interfacial energy between the particle surface and the aqueous media. The nanoparticles showed an excellent dispersive stability under storage in a phosphate-buffered saline solution for 12 days. The positive zeta-potentials for the nanoparticles decreased and changed to negative values with increasing plasmid DNA (pDNA) content. Agarose gel electrophoresis showed that the complex formation between the nanoparticles and the pDNA coincided with the zeta-potential results. The results of in vitro transfection and cell viability on HEK 293 cells indicated that the nanoparticles could be used as gene delivery carriers.
authors.affiliation: College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Yongbong-dong, Bug-gu, Gwangju 500-757, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Kim
authors.forename: Soo-Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Park
authors.forename: Yong-Bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Kang Choon
authors.initials: KC
authors.lastname: Lee
authors.forename: In-Joon
authors.initials: IJ
authors.lastname: Oh
mesh_terms: D002470:Cell Survival; D016503:Drug Delivery Systems; D004355:Drug Stability; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D049329:Nanostructures; D010316:Particle Size; D011091:Polyesters; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection
chemical_list: D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D011094:Polyethyleneimine