title: [Identification and sequence analysis of a novel HLA-B*5614 allele].
abstract: OBJECTIVE
  To investigate the molecular genetics basis for a novel HLA allele, HLA-B*5614, in Chinese population.
  
  
  METHODS
  DNA was extracted from whole blood by salting-out method. The HLA-B exons 2-4 of the proband was amplified and the amplified product was cloned using TOPO cloning sequencing kit to split the two alleles apart. Both strands of exons 2,3 and 4 of chosen colonies were sequencing. The PCR-SSP was performed to confirm the mutations detected by sequencing.
  
  
  RESULTS
  The sequencing results showed the HLA-B alleles of the proband as B*1502 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY601726, AY610727, AY610728). After BLAST analysis, the novel allele differs from B*5608 by a single nucleotide at position 277G-->C in exon 2. This results in an amino acid change from Gly to Arg at codon 93.
  
  
  CONCLUSIONS
  This allele is a novel allele and has been officially named B*5614 by the WHO Nomenclature Committee.
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, 310006, PR China. zfm00@hotmail.com
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Qin-feng
authors.initials: QF
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hai-qin
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Qi-hua
authors.initials: QH
authors.lastname: Fu
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D005091:Exons / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D015235:HLA-B Antigens