title: Single-feature polymorphism discovery in the barley transcriptome.
abstract: A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10,000 single-feature polymorphisms (SFP) between two barley genotypes. The method has good sensitivity, as 67% of known single-nucleotide polymorphisms (SNP) were called as SFPs. This method is applicable to all oligonucleotide microarray data, accounts for SNP effects in gene-expression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes.
authors.affiliation: Scottish Crop Research Institute, Genome Dynamics, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
authors.forename: Nils
authors.initials: N
authors.lastname: Rostoks
authors.forename: Justin O
authors.initials: JO
authors.lastname: Borevitz
authors.forename: Peter E
authors.initials: PE
authors.lastname: Hedley
authors.forename: Joanne
authors.initials: J
authors.lastname: Russell
authors.forename: Sharon
authors.initials: S
authors.lastname: Mudie
authors.forename: Jenny
authors.initials: J
authors.lastname: Morris
authors.forename: Linda
authors.initials: L
authors.lastname: Cardle
authors.forename: David F
authors.initials: DF
authors.lastname: Marshall
authors.forename: Robbie
authors.initials: R
authors.lastname: Waugh
mesh_terms: D020869:Gene Expression Profiling; D018745:Genome, Plant / Q000235:genetics; D001467:Hordeum / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D018749:RNA, Plant