title: GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions.
abstract: OBJECTIVE
  To investigate the inhibitory effect of a new compound of GLB on tumor metastasis in vivo and analyze its actions on tumor cell adhesion to clarify its mechanism.
  
  
  METHODS
  The effect of GLB on tumor metastasis was analyzed by Lewis lung carcinoma model. The pathological morphology of lung alveolar was evaluated by hematoxylin-eosin staining. The effect of GLB on the proliferation of human prostate cancer cell (PC-3M, with a high metastatic characteristic) was studied using the MTT method, and its actions on PC-3M cell adhesion to human umbilical vein endothelial cells (HUVEC) and laminin were analyzed in vitro.
  
  
  RESULTS
  GLB (100 mg/kg/d for 28 d, ig) reduced the number of lung colonies of Lewis lung carcinoma metastasis significantly (P<0.05). Simultaneously, GLB could mitigate the damage of lung alveolar caused by metastasic tumor deposits. In vitro, GLB inhibited dramatically the adhesion of PC-3M cells to HUVEC (P< 0.01) and laminin (P<0.05), without cytotoxic or anti-proliferative action on PC-3M cells.
  
  
  CONCLUSIONS
  GLB has anti-tumor metastatic activity, which partly depends on its inhibition of tumor adhesion.
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences and State Key Laboratory of Natural Biomimetic Drugs, Peking University, Beijing 100083, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pan
authors.forename: Qian-Liu
authors.initials: QL
authors.lastname: Song
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Lu
authors.forename: He-Ming
authors.initials: HM
authors.lastname: Yu
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D018827:Carcinoma, Lewis Lung / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000502:physiology; D005260:Female; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010069:Oxadiazoles / Q000737:chemistry / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology*; D013141:Spiro Compounds / Q000737:chemistry / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C512376:(2''S,3aR,6S,7aR)-3''-acetyl-3a,7a-dihydro-2,2,2'-2',-tetramethyl-5'-(4-bromophenyl)-spiro(spiro(1,3-dioxolo(4,5-D)-pyrane-6(7H),5'(4'H)-1',3'-dioxolo)-7(6H),2''(3''H)-(1,3,4)-oxadiazole); D000970:Antineoplastic Agents; D007797:Laminin; D010069:Oxadiazoles; D011714:Pyrans; D013141:Spiro Compounds