title: Enantioselective capillary electrophoretic separation of tryptophane- and tyrosine-methylesters in a dual system with a tetra-oxadiaza-crown-ether derivative and a cyclodextrin.
abstract: Different dual selector systems containing a cyclodextrin derivative (methyl-beta-cyclodextrin and dimethyl-beta-cyclodextrin) and a new diaza-crown-ether derivative (N-[2-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecan-7-yl)propanoyl]glycine) were studied in the enantioselective separation of tryptophan-methylester and tyrosine-methylester enantiomers. This paper deals with the systematic study of the effects of changing the composition of the background electrolyte on the resolution of the d- and l- forms using an experimental design approach. It was found that the dual systems allowed a better chiral separation of the amino acid derivatives. The experimental design approach also allowed improving the separation compared to the starting conditions (center point of the design), which were adopted from a previous study.
authors.affiliation: Research Group of Homogeneous Catalysis Hungarian Academy of Sciences, University of Debrecen, P.O. Box 7, Debrecen H-4010, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Elek
authors.forename: Debby
authors.initials: D
authors.lastname: Mangelings
authors.forename: Timea
authors.initials: T
authors.lastname: Iványi
authors.forename: István
authors.initials: I
authors.lastname: Lázár
authors.forename: Y Vander
authors.initials: YV
authors.lastname: Heyden
mesh_terms: D000465:Algorithms; D002021:Buffers; D020650:Combinatorial Chemistry Techniques; D047248:Crown Compounds / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification
chemical_list: D002021:Buffers; D047248:Crown Compounds; D003505:Cyclodextrins; D004573:Electrolytes; C503752:N-(2-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecan-7-yl)propanoyl)glycine; C040444:tyrosine methyl ester; D014443:Tyrosine; C028129:tryptophan methyl ester; D014364:Tryptophan; D005998:Glycine