title: A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.
abstract: Articular cartilage has limited intrinsic repair capacity. In order to promote cartilage repair, the amplification and transfer of autologous chondrocytes using three-dimensional scaffolds have been proposed. We have developed an injectable and self-setting hydrogel consisting of hydroxypropyl methylcellulose grafted with silanol groups (Si-HPMC). The aim of the present work is to assess both the in vitro cytocompatibility of this hydrogel and its ability to maintain a chondrocyte-specific phenotype. Primary chondrocytes isolated from rabbit articular cartilage (RAC) and two human chondrocytic cell lines (SW1353 and C28/I2) were cultured into the hydrogel. Methyl tetrazolium salt (MTS) assay and cell counting indicated that Si-HPMC hydrogel did not affect respectively chondrocyte viability and proliferation. Fluorescent microscopic observations of RAC and C28/I2 chondrocytes double-labeled with cell tracker green and ethidium homodimer-1 revealed that chondrocytes proliferated within Si-HPMC. Phenotypic analysis (RT-PCR and Alcian blue staining) indicates that chondrocytes, when three-dimensionnally cultured within Si-HPMC, expressed transcripts encoding type II collagen and aggrecan and produced sulfated glycosaminoglycans. These results show that Si-HPMC allows the growth of differentiated chondrocytes. Si-HPMC therefore appears as a potential scaffold for three-dimensional amplification and transfer of chondrocytes in cartilage tissue engineering.
authors.affiliation: INSERM EM 9903, Research Center on Materials with Biological Interest, School of Dental Surgery, University of Nantes, 1-Place Alexis Ricordeau 44042, Nantes Cedex 1, France.
authors.forename: C
authors.initials: C
authors.lastname: Vinatier
authors.forename: D
authors.initials: D
authors.lastname: Magne
authors.forename: P
authors.initials: P
authors.lastname: Weiss
authors.forename: C
authors.initials: C
authors.lastname: Trojani
authors.forename: N
authors.initials: N
authors.lastname: Rochet
authors.forename: G F
authors.initials: GF
authors.lastname: Carle
authors.forename: C
authors.initials: C
authors.lastname: Vignes-Colombeix
authors.forename: C
authors.initials: C
authors.lastname: Chadjichristos
authors.forename: P
authors.initials: P
authors.lastname: Galera
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D019281:Dimerization; D006025:Glycosaminoglycans / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012821:Silanes / Q000737:chemistry*; D013997:Time Factors; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D020100:Hydrogels; D012333:RNA, Messenger; D012821:Silanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D065347:Hypromellose Derivatives; D008747:Methylcellulose