title: Molecular characterization of Entamoeba histolytica RNase III and AGO2, two RNA interference hallmark proteins.
abstract: Entamoeba histolytica, a protozoan parasite with variable DNA content and complex ploidity, has defied most efforts aimed at gene depletion using classical genetic methods. In this study, we identified and characterized two proteins involved in the RNA interference (RNAi) pathway, RNase III and AGO2. Our results strengthen the findings that an RNAi pathway does exist in this parasite.
authors.affiliation: Department of Molecular Microbiology, The Bruce, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, POB 9649, Haifa 31096, Israel.
authors.forename: Mona
authors.initials: M
authors.lastname: Abed
authors.forename: Serge
authors.initials: S
authors.lastname: Ankri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D015536:Down-Regulation; D004748:Entamoeba histolytica / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000737:chemistry; D016053:RNA, Protozoan / Q000737:chemistry; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000235:genetics*; D043244:Ribonuclease III / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D012330:RNA, Double-Stranded; D016053:RNA, Protozoan; D034743:RNA-Induced Silencing Complex; D043244:Ribonuclease III