[Recovery and purification of monoclonal antibody producted by continuous perfusion culture using expended bed adsorption].
Monoclonal antibody producted by continuous perfusion culture was recovered and purified by expended bed adsorption chromatography. A packed bed column XK16/20 was used for method scouting with Streamline SP adsorbent. Two expended bed columns Streamline-25 and -50 were used for method optimization and pilot scale experiment, respectively. The recovery yield of monoclonal antibody was above 90% in a 5 - 7 fold enhanced purity and 10 fold increased concentration. According to the different concentration of monoclonal antibody in cell culture broth, about 18 - 50L fluid can be treated in a single cycle. MAb purification from lab scale (400mg per cycle) to a small pilot scale (2g per cycle) has been achieved. Compared with packed bed adsorption, the preparation cycle was half shortened, and the cost of production and the complexity of process were decreased markedly. It has been proven that a purification process based on expended bed adsorption technique is simple, efficient and economical.
Yu
Xiao-Ling
XL
The Fourth military medical university, National Engineering Research Center, Xi'an 710032, China.
Mi
Li
L
Yao
Xi-Ying
XY
Chen
Zhi-Nan
ZN
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002845:Chromatography / Q000379:methods*; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism
D000911:Antibodies, Monoclonal