Ball lens coupled fiber-optic probe for depth-resolved spectroscopy of epithelial tissue.
A ball lens coupled fiber-optic probe design is described for depth-resolved measurements of the fluorescence and reflectance properties of epithelial tissue. A reflectance target, fluorescence targets, and a two-layer tissue phantom consisting of fluorescent microspheres suspended in collagen are used to characterize the performance of the probe. Localization of the signal to within 300 microm of the probe tip is observed by use of reflectance and fluorescence targets in air. Differential enhancement of the fluorescence signal from the top layer of the two-layer tissue phantom is observed.
Schwarz
Richard A
RA
Department of Biomedical Engineering, University of Texas at Austin, 1 University Station, C0800, Austin, Texas 78712, USA.
Arifler
Dizem
D
Chang
Sung K
SK
Pavlova
Ina
I
Hussain
Insiya A
IA
Mack
Vivian
V
Knight
Bob
B
Richards-Kortum
Rebecca
R
Gillenwater
Ann M
AM
D000818:Animals; D004848:Epithelium / Q000502:physiology* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D055100:Optical Fibers; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods; D014159:Transducers