Structural dissection and high-throughput screening of mannosylglycerate synthase.
The enzymatic transfer of activated mannose yields mannosides in glycoconjugates and oligo- and polysaccharides. Yet, despite its biological necessity, the mechanism by which glycosyltransferases recognize mannose and catalyze its transfer to acceptor molecules is poorly understood. Here, we report broad high-throughput screening and kinetic analyses of both natural and synthetic substrates of Rhodothermus marinus mannosylglycerate synthase (MGS), which catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS indicates that it is at the cusp of inverting and retaining transferases. The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and catalytic sites, unveil the mannosyl transfer center. Nucleotide specificity is as important in GDP-D-mannose recognition as the nature of the donor sugar.
Flint
James
J
Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, The Medical School, Newcastle upon Tyne NE2 4HH, UK.
Taylor
Edward
E
Yang
Min
M
Bolam
David N
DN
Tailford
Louise E
LE
Martinez-Fleites
Carlos
C
Dodson
Eleanor J
EJ
Davis
Benjamin G
BG
Gilbert
Harry J
HJ
Davies
Gideon J
GJ
D003461:Crystallography; D006017:Glycolipids / Q000378:metabolism; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D007700:Kinetics; D008364:Mannosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D013058:Mass Spectrometry; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D011487:Protein Conformation; D045245:Rhodothermus / Q000201:enzymology*
C483043:2-O-mannosylglycerate; D006017:Glycolipids; D006155:Guanosine Diphosphate Mannose; D008364:Mannosyltransferases; C402687:mannosylglycerate synthase