title: Investigation of novel mediators for a glucose biosensor based on metal picolinate complexes.
abstract: The metal complexes [Os(byp)(2)(pic)](+) and [Ru(byp)(2)(pic)](+) where byp is 2,2'-bipyridine and HPic is o-picolinic acid were synthesised and characterised using spectroscopic and electrochemical techniques. These complexes were then evaluated as mediators for a glucose oxidase (GOx)-based biosensor. Results demonstrate the electrocatalytic behaviour of both metal couples towards regeneration of the flavoprotein GOx (FADH(2)) group, when co-immobilised with glucose oxidase. Surface immobilisation was achieved by potential cycling in aqueous solutions of the metal complexes at a glucose oxidase (GOx)/Nafion modified electrode. This proved successful in terms of catalytic efficiency and stability of redox sites. Kinetic parameters associated with both enzymatic and mediator reactions were estimated and the stability/performance properties of the sensor were tested.
authors.affiliation: Electrochemical Technology Research Centre, Dept. Applied Science, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland.
authors.forename: Susan
authors.initials: S
authors.lastname: Warren
authors.forename: Timothy
authors.initials: T
authors.lastname: McCormac
authors.forename: Eithne
authors.initials: E
authors.lastname: Dempsey
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000737:chemistry*; D010848:Picolinic Acids / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D004800:Enzymes, Immobilized; D008670:Metals; D010848:Picolinic Acids; D005949:Glucose Oxidase; D005947:Glucose; C030614:picolinic acid