[Ginsenoside Rbl attenuates beta-amyloid peptide25-35 -induced tau hyperphosphorylation in cortical neurons].
OBJECTIVE
To explore the effect and the possible mechanism of ginsenoside Rb1 on beta-amyloid peptide (beta-AP)(25-35) -induced tau protein hyperphosphorylation in cortical neurons.


METHODS
Western blotting and immunocytochemical staining were used to detect tau phosphorylation level, total tau and glycogen synthase kinase-3beta (GSK-3beta) in cortical neurons.


RESULTS
After exposure to beta-AP(25-35) (20 micromol x L(-1)) for 12 h, the levels of tau protein phosphorylation in the sites of Ser 396, Ser 199/202, Thr 231 and total tau were raised. Meanwhile, the expression of GSK-3beta also increased. Pretreatment with ginsenoside Rbl or lithium chloride, a specific inhibitor of GSK-3beta, markedly reduced beta-AP(25-35)-induced tau hyperphosphorylation and the expression of GSK-3beta.


CONCLUSIONS
Ginsenoside Rb1 can attenuate beta AP(25-35)-induced tau protein hyperphosphorylation in cortical neurons by inhibiting the expression of GSK-3beta.
Zeng
Yu-qi
YQ
Fujian Institute of Geriatrics, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
Chen
Xiao-chun
XC
Zhu
Yuan-gui
YG
Li
Yong-kun
YK
Peng
Xiao-song
XS
Chen
Li-min
LM
Shen
Jie
J
Huang
Tian-wen
TW
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D009474:Neurons / Q000378:metabolism; D005894:Panax / Q000737:chemistry; D010446:Peptide Fragments / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D016875:tau Proteins / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D036145:Ginsenosides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D016875:tau Proteins; C442759:ginsenoside Rb1; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3