Coexistence of salusin and vasopressin in the rat hypothalamo-hypophyseal system.
Salusins are two newly discovered TOR-related peptides consisting of 28 and 20 amino acids and designated salusin-alpha and salusin-beta, respectively. Using immunohistochemistry techniques, salusin-like immunoreactivity was detected in the rat hypothalamo-neurohypophyseal tract and immunopositive cells were distributed in the suprachiasmatic, supraoptic and paraventricular nucleus. In the paraventricular nucleus, salusin-like immunoreactivity was observed both in parvocellular and magnocellular neurons. Many salusin-positive nerve fibers and their terminals were identified in the internal layer of the median eminence and posterior pituitary. Less intense salusin-positive staining of fibers and terminals was found in the suprachiasmatic nucleus and external layer of the median eminence. Dual immunostaining was performed to determine if salusin coexisted with vasopressin or oxytocin in the hypothalamus. Most of the salusin-like immunoreactivity was detected in vasopressin- but not in oxytocin-containing neurons in these nuclei. The functional significance of the coexistence of salusin with vasopressin is discussed, including the possibility that salusin participates in the regulation of blood pressure.
Takenoya
Fumiko
F
Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Hori
Tomoko
T
Kageyama
Haruaki
H
Funahashi
Hisayuki
H
Takeuchi
Masao
M
Kitamura
Yoshitaka
Y
Shichiri
Masayoshi
M
Shioda
Seiji
S
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D007030:Hypothalamo-Hypophyseal System / Q000033:anatomy & histology / Q000378:metabolism*; D007031:Hypothalamus / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013493:Suprachiasmatic Nucleus / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000378:metabolism; D014661:Vasoconstriction / Q000502:physiology; D014667:Vasopressins / Q000378:metabolism*
D009479:Neuropeptides; D014667:Vasopressins; D010121:Oxytocin; D000251:Adenosine Triphosphatases