title: Depletion and fractionation technologies in plasma proteomic analysis.
abstract: This review intends to survey the traditional and current technologies in the depletion and subfractionation of plasma proteins for further analyses. The value of depletion aims to enrich low-abundant proteins by removing highly abundant proteins, such as albumin or immunoglobulin G, from plasma. With this approach, one can examine both the resulting high- and low-abundant protein fractions. The depleted protein population can be further subfractionated based on their isoelectric point ranges, creating a more discrete pool of proteins for detailed post-translational modification studies by methods such as 2D gel electrophoresis and mass spectrometry. The concept of divide to conquer will greatly enhance our ability to identify and characterize low-abundant proteins and cleaved peptides from plasma as important diagnostic markers or potential drug targets. This can potentially reverse the decline in the development of new plasma diagnostic tests.
authors.affiliation: Charles River Proteomic Services, 57 Union Street, Worcester, MA 01608, USA. sunny.tam@dds.criver.com
authors.forename: Sun W
authors.initials: SW
authors.lastname: Tam
authors.forename: John
authors.initials: J
authors.lastname: Pirro
authors.forename: Douglas
authors.initials: D
authors.lastname: Hinerfeld
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D040901:Proteomics / Q000379:methods*
chemical_list: D001798:Blood Proteins