title: Does resveratrol prevent free radical-induced acute pancreatitis?
abstract: OBJECTIVE
  The purpose of this study was to examine protective and antioxidative effect of stilbene derivatives, resveratrol and diethylstilbestrol, in experimental acute pancreatitis (EAP).
  
  
  METHODS
  EAP was induced in male Wistar rats by retrograde injection of tert-butyl hydroperoxide (ButOOH) solution, a well-known prooxidant agent, into the common bile pancreatic duct. After a 3-hour observation, the animals were killed. Blood samples were collected. Each pancreas was removed and weighed. Tissue samples were taken for microscopic studies. The carbonyl and sulfhydryl (SH) group levels were estimated in the homogenate.
  
  
  RESULTS
  Examination using light microscopy revealed morphologic changes in pancreata removed from EAP rats, namely focal edema, acinar cell vacuolization, and focal necrosis of pancreatic acini. The electron microscopic analysis also showed changes in their subcellular structures: dilated cisternae of the rough endoplasmic reticulum, swollen mitochondria, and "debris" of mitochondrial cristae. These changes corresponded with higher activities of serum amylase and tissue carbonyl groups levels and decreased SH group level compared with controls. Changes in pancreata were much less pronounced in the rats that received resveratrol or diethylstilbestrol for 8 days prior to ButOOH injection.
  
  
  CONCLUSIONS
  Stilbene derivatives prevent pancreatic cells from structural changes during ButOOH-induced acute pancreatitis.
authors.affiliation: Department of General Surgery and Transplantation, Medical University of Gdansk, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Lawinski
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Sledzinski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kubasik-Juraniec
authors.forename: Jan Henryk
authors.initials: JH
authors.lastname: Spodnik
authors.forename: Michal
authors.initials: M
authors.lastname: Wozniak
authors.forename: Wojciech
authors.initials: W
authors.lastname: Boguslawski
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004054:Diethylstilbestrol / Q000494:pharmacology; D005609:Free Radicals; D008297:Male; D010195:Pancreatitis / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D013267:Stilbenes; D004054:Diethylstilbestrol; D020122:tert-Butylhydroperoxide; D000681:Amylases; D000077185:Resveratrol