[The characteristics of nuclear/cytoplasmic genomes of asymmetric somatic hybrid of Daucus carota var. sativa and Swertia mussotii].
Protoplasts of Swertia mussotii irradiated by ultra-violet light (UV) 260 microW/cm(2) were fused with protoplasts of Daucus carota var. sativa using the PEG method. Analysis of 5S rDNA spacer sequences and random amplified polymorphic DNA (RAPD) showed the presence and combination of nuclear DNA from both parents. All hybrids mainly contained genomes of D. carota (receptor) RAPD bands, and a few donor RAPD bands of S. mussotii. There is no obvious relationship between the nuclear DNA composition was not affected by UV-dosage. SSR analysis of chloroplast DNA of the hybrid clones further revealed that cpDNAs of the parents were integrated and recombined in the hybrids randomly.
Li
Zi-Dong
ZD
School of Life Sciences, Shandong Univeristy, Jinan 250100, China.
Shao
Fei
F
Cai
Yun-Fei
YF
Xiang
Feng-Ning
FN
Xia
Guang-Min
GM
D002678:Chimera / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D018552:Daucus carota / Q000235:genetics* / Q000528:radiation effects; D018745:Genome, Plant / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D029967:Swertia / Q000235:genetics* / Q000528:radiation effects
D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S