title: Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique.
abstract: We have recently developed an organ manufacturing technique that enables us to form cell/biomaterial complex three-dimensional (3D) architectures in designed patterns. This technique employs a highly accurate 3D micropositioning system with a pressue-controlled syringe to deposit cell/biomaterial structures with a lateral resolution of 10 microm. The pressure-activated micro-syringe is equipped with a fine-bore exit needle using which a wide variety of 3D patterns with different arrays of channels (through-holes) were created. The channels can supply living cells with nutrients and allow removing the cell metabolites. The embedded cells remain viable and perform biological functions as long as the 3D structures are retained. The new technology has the potential for eventual high-throughput production of artificial human tissues and organs.
authors.affiliation: Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Yan
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Pan
authors.forename: Haixia
authors.initials: H
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Rendong
authors.initials: R
authors.lastname: Wu
authors.forename: Renji
authors.initials: R
authors.lastname: Zhang
authors.forename: Qingping
authors.initials: Q
authors.lastname: Lu
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry; D046508:Culture Techniques; D005780:Gelatin / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D008422:Materials Testing; D008856:Microscopy, Fluorescence; D011312:Pressure; D051381:Rats; D012984:Software; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D048271:Chitosan