title: Central European Dobrava Hantavirus isolate from a striped field mouse (Apodemus agrarius).
abstract: Dobrava virus (DOBV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS) in Europe. It is hosted by at least two rodent species, Apodemus flavicollis and A. agrarius. According to their natural hosts they form the distinct genetic lineages DOBV-Af and DOBV-Aa, respectively. We have now established a DOBV isolate named Slovakia (SK/Aa) from an A. agrarius animal captured in Slovakia. The complete S and M and partial L segment nucleotide sequences of the new isolate were determined. Phylogenetic analyses showed that the SK/Aa isolate clustered together with the other DOBV-Aa sequences amplified from A. agrarius before and can be taken as the representative of this genetic lineage. SK/Aa, in comparison with a DOBV-Af isolate, was used for serotyping neutralizing antibodies of HFRS patients in Central Europe. Most patients' sera exhibited a higher endpoint titer when probed with our new isolate, suggesting that DOBV-Aa strains are responsible for most of the DOBV-caused HFRS cases in this region.
authors.affiliation: Institute of Virology, Helmut Ruska Haus, Charité School of Medicine, Humboldt University, D-10098 Berlin, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Klempa
authors.forename: Michal
authors.initials: M
authors.lastname: Stanko
authors.forename: Milan
authors.initials: M
authors.lastname: Labuda
authors.forename: Rainer
authors.initials: R
authors.lastname: Ulrich
authors.forename: Helga
authors.initials: H
authors.lastname: Meisel
authors.forename: Detlev H
authors.initials: DH
authors.lastname: Krüger
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000662:veterinary* / Q000821:virology; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D009115:Muridae / Q000382:microbiology*; D010802:Phylogeny; D012376:Rodent Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D018154:Slovakia
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral