title: Interdependence between transcription and mRNP processing and export, and its impact on genetic stability.
abstract: The conserved eukaryotic THO-TREX complex acts at the interface between transcription and mRNA export and affects transcription-associated recombination. To investigate the interdependence of nuclear mRNA processes and their impact on genomic integrity, we analyzed transcript accumulation and recombination of 40 selected mutants covering representative steps of the biogenesis and export of the messenger ribonucleoprotein particle (mRNP). None of the mutants analyzed shared the strong transcript-accumulation defect and hyperrecombination of THO mutants. Nevertheless, mutants in 3' end cleavage/polyadenylation, nuclear exosome, and mRNA export showed a weak but significant effect on recombination and transcript accumulation. Mutants of the nuclear exosome (rrp6) and 3' end processing factors (rna14 and rna15) showed inefficient transcription elongation and genetic interactions with THO. The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Luna
authors.forename: Sonia
authors.initials: S
authors.lastname: Jimeno
authors.forename: Mercedes
authors.initials: M
authors.lastname: Marín
authors.forename: Pablo
authors.initials: P
authors.lastname: Huertas
authors.forename: María
authors.initials: M
authors.lastname: García-Rubio
authors.forename: Andrés
authors.initials: A
authors.lastname: Aguilera
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D042822:Genomic Instability; D016296:Mutagenesis; D012319:RNA Polymerase II / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D012331:RNA, Fungal; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D012319:RNA Polymerase II