title: A B220+ CD117+ CD19- hematopoietic progenitor with potent lymphoid and myeloid developmental potential.
abstract: In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220+ CD117+ CD19- NK1.1- cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1-0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5-10 sorted cells formed B cells, 1 in 10-15 formed T cells and 1 in 5-10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta-like-1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19- NK1.1- cells of wild-type mice and other multi-lineage BM progenitors is discussed.
authors.affiliation: Developmental and Molecular Immunology, Center for Biomedicine Department of Clinical and Biological Sciences (DKBW), University of Basel, Basel, Switzerland.
authors.forename: Gina
authors.initials: G
authors.lastname: Balciunaite
authors.forename: Rod
authors.initials: R
authors.lastname: Ceredig
authors.forename: Steffen
authors.initials: S
authors.lastname: Massa
authors.forename: Antonius G
authors.initials: AG
authors.lastname: Rolink
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D018941:Antigens, CD19 / Q000096:biosynthesis*; D000950:Antigens, Ly; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens / Q000096:biosynthesis*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000276:immunology; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000941:Antigens; D018941:Antigens, CD19; D000950:Antigens, Ly; D000954:Antigens, Surface; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens