title: Antisperm antibodies in semen, sera and follicular fluids of infertile patients: relation to reproductive outcome after in vitro fertilization.
abstract: OBJECTIVE
  Data given in the literature, regarding the influence of antisperm antibodies (ASA) in the semen and/or sera on in vitro fertilization (IVF) procedure outcome are controversial. The aim of this study was to compare the prevalence of ASA in semen, peripheral blood and follicular fluid as well as to determine total immunoglobulin concentration in the serum and follicular fluid. Selected parameters were analyzed with regard to IVF outcome.
  
  
  METHODS
  The study enrolled 52 married couples. ASA in the semen was determined by direct immunobead mixed antiglobulin reaction (MAR Screen test), while in the peripheral blood and follicular fluid was determined by indirect immunobead MAR Screen test. Immunoglobulin (IgG, IgM and IgA) concentration in the follicular fluid and serum was determined by a liquid-phase immunoprecipitation assay with nephelometric end-point detection and analyzed with regard to IVF outcome.
  
  
  RESULTS
  Semen MAR test IgG was < 20% in 38 couples, and > 20% in 14 couples. Fertilization (73.2% versus 71.5%) and pregnancy rates (28.9% versus 28.57%) in both groups of patients were not significantly different. The results of direct and indirect MAR test were not associated with fertilization and pregnancy rates. Total serum IgG, IgM and IgA in infertile women were within normal ranges. Follicular fluid IgG was within normal values for serum samples, while IgA and IgM were decreased.
  
  
  CONCLUSIONS
  The presence of ASA on sperm or in the serum and follicular fluid was not associated with IVF outcome in the couples with good quality semen characteristic.
authors.affiliation: Department of Obstetrics and Gynecology, Medical School, University of Zagreb, Sveti Duh Hospital, Zagreb. sanja_vujisic@hotmail.com
authors.forename: Sanja
authors.initials: S
authors.lastname: Vujisić
authors.forename: Snjezana Zidovec
authors.initials: SZ
authors.lastname: Lepej
authors.forename: Lidija
authors.initials: L
authors.lastname: Jerković
authors.forename: Ivana
authors.initials: I
authors.lastname: Emedi
authors.forename: Bozica
authors.initials: B
authors.lastname: Sokolić
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000276:immunology*; D006801:Humans; D007246:Infertility / Q000276:immunology* / Q000628:therapy; D007247:Infertility, Female / Q000276:immunology / Q000628:therapy; D007248:Infertility, Male / Q000276:immunology / Q000628:therapy; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D000906:Antibodies