The caprine oxyhemoglobin dissociation curve.
The caprine oxyhemoglobin dissociation curve has not been previously defined. Blood from 10 healthy goats was equilibrated in a tonometer with calibrated gas mixtures of oxygen at concentrations of 95%, 21%, 13%, 12%, 10%, 9%, 8%, 5%, 4%, and 2.5% , 5% carbon dioxide, balance nitrogen. The pH, partial pressure of oxygen (PO2), partial pressure of carbon dioxide (PCO2), total hemoglobin, oxyhemoglobin saturation, carboxyhemoglobin, methemoglobin, and oxygen content were measured. The PO2/oxyhemoglobin and the PO2/oxygen content relationships were graphed with curve-fitting software and a formula for calculating oxyhemoglobin from PO2 was generated. The maximum oxygen content per gram of hemoglobin was 1.29 ml of oxygen per gram of hemoglobin. The PO2 at which hemoglobin was 50% saturated (P50) from the PO2/oxyhemoglobin relationship was 28.6+/-1.5 mmHg and that from the PO2/oxygen content relationships was 29.1+/-1.6 mmHg. The Hill coefficient for the PO2/oxyhemoglobin data was 3.0+/-0.4.
Haskins
Steve C
SC
Department of Surgical and Radiological Science, School of Veterinary Medicine, University of California, Davis, CA 95616-8745, USA. schaskins@ucdavis.edu
Rezende
Marlis L
ML
D000818:Animals; D006041:Goats / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism*; D010313:Partial Pressure
D010108:Oxyhemoglobins; D010100:Oxygen