title: Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome.
abstract: Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.
authors.affiliation: Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. B.W.M.vanBalkom@vet.uu.nl
authors.forename: Bas W M
authors.initials: BW
authors.lastname: van Balkom
authors.forename: Renske A
authors.initials: RA
authors.lastname: van Gestel
authors.forename: Jos F H M
authors.initials: JF
authors.lastname: Brouwers
authors.forename: Jeroen
authors.initials: J
authors.lastname: Krijgsveld
authors.forename: Aloysius G M
authors.initials: AG
authors.lastname: Tielens
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Jaap J
authors.initials: JJ
authors.lastname: van Hellemond
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis* / Q000502:physiology; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012550:Schistosoma mansoni / Q000737:chemistry*
chemical_list: D015801:Helminth Proteins; D020543:Proteome