Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome.
Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.
van Balkom
Bas W M
BW
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. B.W.M.vanBalkom@vet.uu.nl
van Gestel
Renske A
RA
Brouwers
Jos F H M
JF
Krijgsveld
Jeroen
J
Tielens
Aloysius G M
AG
Heck
Albert J R
AJ
van Hellemond
Jaap J
JJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis* / Q000502:physiology; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012550:Schistosoma mansoni / Q000737:chemistry*
D015801:Helminth Proteins; D020543:Proteome