title: Congener-specific tissue distribution of aroclor 1254 and a highly chlorinated environmental PCB mixture in rats.
abstract: Polychlorinated biphenyl (PCB) mixtures were synthesized and marketed in Eastern European countries, but little is known abouttheir composition, distribution, ortoxicity. PCB-contaminated soil from the former production site of the Polish PCB mixture Chlorofen was collected, and the PCBs were extracted. An in vivo study was performed to investigate the PCB tissue distribution and biochemical effects of this soil extract in immature male Sprague-Dawley rats. Rats were administered 0.05 mmol/kg soil-extracted PCBs or Aroclor 1254 and sacrificed 7 days later, and congener-specific PCB profiles in selected tissues were determined. Distribution of SigmaPCBs (sum of 120 congeners) in tissues was primarily a function of lipid content of the tissues, except for the spleen, which retained more PCB than other tissues. Multivariate analysis of the PCB congener data showed that (a) congener profiles in tissues had changed, as compared to the parent mixture; (b) disposition and redistribution of individual congeners in vivo differed between both mixtures; and (c) more highly chlorinated congeners were retained in the spleens of both treatment groups. Differences in the induction of cytochrome P-450 1A and 2B subfamilies reflected the homologue composition of the respective mixtures and predict a different toxicity profile for Chlorofen than for Aroclor 1254.
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, University of lowa, Iowa City, Iowa 52242, USA.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kania-Korwel
authors.forename: Keri C
authors.initials: KC
authors.lastname: Hornbuckle
authors.forename: Aaron
authors.initials: A
authors.lastname: Peck
authors.forename: Gabriele
authors.initials: G
authors.lastname: Ludewig
authors.forename: Larry W
authors.initials: LW
authors.lastname: Robertson
authors.forename: Wieslaw W
authors.initials: WW
authors.lastname: Sulkowski
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Espandiari
authors.forename: C Gary
authors.initials: CG
authors.lastname: Gairola
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Lehmler
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism; D006256:Hazardous Waste; D007668:Kidney / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009206:Myocardium / Q000737:chemistry; D011044:Poland; D011078:Polychlorinated Biphenyls / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry; D012989:Soil Pollutants / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D006256:Hazardous Waste; D008055:Lipids; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1