title: Tooth development in vitro in two teleost fish, the cichlid Hemichromis bimaculatus and the cyprinid Danio rerio.
abstract: A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission electron microscopy was used to compare cellular and extracellular features of tooth germs developing in vitro with the situation in vivo. Head explants of H. bimaculatus from 120 h post-fertilization (hPF) to 8.5 days post-fertilization (dPF) and of zebrafish from 45 hPF to 79 hPF and adults kept in culture for 3, 4 or 7 days revealed that tooth germs developed in vitro from explants in which the buccal or pharyngeal epithelium was apparently undifferentiated and, when present at the time of explantation, they continued their development up to a stage of attachment. In addition, the medium allowed the morphogenesis and cytodifferentiation of the tooth germs similar to that observed in vivo and the establishment of a dental pattern (place and order of tooth appearance and of attachment) that mimicked that in vivo. Organotypic culture in serum-free conditions thus provides us with the means of studying epithelial-mesenchymal interactions during tooth development in teleost fish and of analysing the genetic control of either mandibular or pharyngeal tooth development and replacement in these polyphyodont species. Importantly, it allows heads from embryonically lethal (zebrafish) mutants or from early lethal knockdown experiments to develop beyond the point at which the embryos normally die. Such organotypic culture in serum-free conditions could therefore become a powerful tool in developmental studies and open new perspectives for craniofacial research.
authors.affiliation: Biology Department, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Van der Heyden
authors.forename: F
authors.initials: F
authors.lastname: Allizard
authors.forename: J-Y
authors.initials: JY
authors.lastname: Sire
authors.forename: A
authors.initials: A
authors.lastname: Huysseune
mesh_terms: D000818:Animals; D023681:Cichlids / Q000033:anatomy & histology / Q000196:embryology; D016895:Culture Media, Serum-Free; D009805:Odontogenesis / Q000502:physiology*; D046509:Tissue Culture Techniques; D014083:Tooth Germ / Q000254:growth & development* / Q000648:ultrastructure; D015027:Zebrafish / Q000033:anatomy & histology / Q000196:embryology
chemical_list: D016895:Culture Media, Serum-Free