title: [Killing effect of 131I-Herceptin on breast cancer cell lines in vitro].
abstract: OBJECTIVE
  To study the specific killing effect of (131)I-labeled Herceptin on breast cancer cell lines positive for Her-2 expression.
  
  
  METHODS
  Anti-Her-2 monoclonal antibody Herceptin was labeled with (131)I by means of IODO-GEN, and the killing effects of Herceptin on SK-BR-3, MCF-7 and A549 cell lines expressing Her-2 were evaluated with MTT assay.
  
  
  RESULTS
  (131)I-Herceptin had immunoactivity had to that of Herceptin but possessed stronger killing effects on the cell lines expressing Her-2 than Herceptin and (131)I used alone (P<0.05). Its killing effect on cell lines negative or only weakly positive for Her-2 expression was similar to that of (131)I and Herceptin.
  
  
  CONCLUSIONS
  (131)I-Herceptin can specifically kill Her-2-positive cancer cells in vitro.
authors.affiliation: Center of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Rong-cheng
authors.initials: RC
authors.lastname: Luo
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Li
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Zhang
authors.forename: Cheng-wei
authors.initials: CW
authors.lastname: Lü
authors.forename: Xiao
authors.initials: X
authors.lastname: Yan
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000187:drug effects*; D000068878:Trastuzumab; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab