title: Protein microarrays as a discovery tool for studying protein-protein interactions.
abstract: Exploring the function of the genome and the encoded proteins has emerged as a new and exciting challenge in the postgenomic era. Novel technologies come into view that promise to be valuable for the investigation not only of single proteins, but of entire protein networks. Protein microarrays are the innovative assay platform for highly parallel in vitro studies of protein-protein interactions. Due to their flexibility and multiplexing capacity, protein microarrays benefit basic research, diagnosis and biomedicine. This review provides an overview on the basic principles of protein microarrays and their potential to multiplex protein-protein interaction studies.
authors.affiliation: Division of Molecular Genome Analysis (B050), DKFZ Heidelberg, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. U.Korf@DKFZ.de
authors.forename: Ulrike
authors.initials: U
authors.lastname: Korf
authors.forename: Stefan
authors.initials: S
authors.lastname: Wiemann
mesh_terms: D004261:DNA Replication; D006801:Humans; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011494:Protein Kinases / Q000032:analysis; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D020798:Two-Hybrid System Techniques
chemical_list: D043562:Receptors, G-Protein-Coupled; D016159:Tumor Suppressor Protein p53; D011494:Protein Kinases