title: In vitro polyploidy induction in Rhododendron simsii-hybrids.
abstract: Three in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was rather labour intensive. Direct sowing on media enriched with these compounds required less work but was less efficient. Mitotic inhibitors, when added to multiplication media, induced ploidy chimeras of existing genotypes.
authors.affiliation: Department of Plant Genetics and Breeding, Caritasstaat 21, 9090 Melle, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Eeckhout
authors.forename: G
authors.initials: G
authors.lastname: Samyn
authors.forename: E
authors.initials: E
authors.lastname: Van Bockstaele
mesh_terms: D002678:Chimera; D003470:Culture Media; D005838:Genotype; D018525:Germination; D066298:In Vitro Techniques; D008938:Mitosis; D011123:Polyploidy; D029793:Rhododendron / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D003470:Culture Media