Electrochemical genosensing of the interaction between the potential chemotherapeutic agent, cis-bis(3-aminoflavone)dichloroplatinum(II) and DNA in comparison with cis-DDP.
The interaction of cis-diamminedichloroplatinum(II) (cis-DDP) and the potential novel chemotherapeutic agent, cis-bis(3-aminoflavone)dichloroplatinum(II) (cis-BAFDP) was studied electrochemically with calf thymus double-stranded DNA (dsDNA) by using differential pulse voltammetry (DPV) with disposable pencil graphite electrode (PGE) at the surface. These studies were prompted by beneficial biological properties of cis-BAFDP in comparison with cis-DDP, which were proven in vitro both in human normal and cancer cells and in vivo. The changes in the experimental parameters such as the concentration of cis-DDP and cis-BAFDP were studied by using DPV; in addition, the reproducibility of this genosensor and the detection limit for each compound were determined. After the interaction of cis-DDP with dsDNA, the DPV signal of guanine and adenine was found to be decreasing. In comparison with cis-DDP, a dramatic decrease at adenine signal was also obtained after the interaction of cis-BAFDP and dsDNA. Similar results were also found in solution phase after the latter compound interacts with poly[A]. The features of the proposed electrochemical method for the detection of cis-BAFDP with DNA in comparison with cis-DDP are discussed and compared with those methods previously reported for the other type of DNA-targeted agents in the literature.
Erdem
Arzum
A
Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, Bornova, 35100 Izmir, Turkey. erdema@pharm.ege.edu.tr
Kosmider
Beata
B
Osiecka
Regina
R
Zyner
Elzbieta
E
Ochocki
Justyn
J
Ozsoz
Mehmet
M
D000225:Adenine / Q000737:chemistry; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000235:genetics*; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013501:Surface-Active Agents; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D013501:Surface-Active Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C488623:cis-bis(3-aminoflavone)dichloroplatinum(II); D006108:Graphite; D004247:DNA; C022616:thiazolyl blue; D000225:Adenine; D002945:Cisplatin