title: Detection of West Nile viral RNA from an overwintering pool of Culex pipens pipiens (Diptera: Culicidae) in New Jersey, 2003.
abstract: In total, 1,324 Culex pipiens pipiens L. female mosquitoes were collected at Ft. Hancock, Monmouth County, New Jersey, from January to March 2001-2003. Mosquitoes were held in an insectary at 27 degrees C and a photoperiod of 16:8 (L:D) h for 6 to 21 d after which they were tested in 34 pools. West Nile viral RNA was detected in one pool by a TaqMan reverse transcription-polymerase chain reaction assay; however, infectious virus could not be isolated using either Vero cell plaque assay or C6/36 mosquito cells. Twenty females dissected in January and March 2003 confirmed ovarian diapause status. We suggest that the mode of infection in this pool of overwintering females may have been due to vertical (transgenerational) transmission.
authors.affiliation: Mosquito Research and Control, Department of Entomology, Rutgers University, New Brunswick, NJ 08901- 8536, USA. farajoll@rci.rutgers.edu
authors.forename: Ary
authors.initials: A
authors.lastname: Farajollahi
authors.forename: Wayne J
authors.initials: WJ
authors.lastname: Crans
authors.forename: Patricia
authors.initials: P
authors.lastname: Bryant
authors.forename: Bruce
authors.initials: B
authors.lastname: Wolf
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Burkhalter
authors.forename: Marvin S
authors.initials: MS
authors.lastname: Godsey
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Aspen
authors.forename: Roger S
authors.initials: RS
authors.lastname: Nasci
mesh_terms: D000818:Animals; D003465:Culex / Q000821:virology*; D005260:Female; D009515:New Jersey; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D013997:Time Factors; D014902:West Nile virus / Q000235:genetics*
chemical_list: D012367:RNA, Viral