title: [Detection of CCAAT/enhancer binding protein alpha gene mutations in acute myeloid leukemia].
abstract: OBJECTIVE
  To explore the relationship between CCAAT/enhancer binding protein alpha (C/EBPalpha) gene mutations and the development of acute myeloid leukemia (AML).
  
  
  METHODS
  The whole coding region of C/EBPalpha gene were screened in 48 cases of AML and 11 normal subjects by PCR-single strand conformation polymorphism (PCR-SSCP) and sequencing.
  
  
  RESULTS
  C/EBPalpha mutations were detected in 5 of 48 AML patients. Four duplications and 1 deletion were confirmed by DNA sequencing. All of those are newly identified mutations.
  
  
  CONCLUSIONS
  Different mutation types of C/EBPalpha gene exist in a small number of patients with AML and might be related to the pathogenesis of some leukemias.
authors.affiliation: Institute of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Jin-mei
authors.initials: JM
authors.lastname: Zhao
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Zhi-fang
authors.initials: ZF
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Ge
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics*; D002648:Child; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha