Interactions of drugs and an oligonucleotide with charged membranes analyzed by immobilized liposome chromatography.
We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or distearoyltrimethylammoniumpropane) or detergent (sodium dodecylsulfate) interacted electrostatically in a concentration-dependent matter with the solutes. The oligonucleotide ions presumably bound to the liposomes by multipoint interactions, which was saturable. Sodium dodecylsulfate seemed to affect the drug-membrane interactions more strongly than phosphatidylserine did, probably due to different positioning in the bilayer.
Lundquist
Anna
A
Department of Biochemistry, Biomedical Center, Uppsala University, Box 576, SE-751 23 Uppsala, Sweden.
Engvall
Caroline
C
Boija
Elisabet
E
Kurtovic
Sanela
S
Chattopadhyaya
Jyoti
J
Hägglund
Christine Lagerquist
CL
Lundahl
Per
P
D001483:Base Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D008081:Liposomes; D008567:Membranes, Artificial; D009841:Oligonucleotides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*
D008081:Liposomes; D008567:Membranes, Artificial; D009841:Oligonucleotides; D004364:Pharmaceutical Preparations