Extracorporeal venovenous cooling for induction of mild hypothermia in human-sized swine.
OBJECTIVE
Several cooling methods have been investigated for inducing mild hypothermia (33-36 degrees C) after cardiac arrest, brain trauma, or stroke. To achieve its best effect, therapeutic hypothermia has to be applied very early after the ischemic insult; otherwise, the beneficial effect would be diminished or even abrogated. The aim of this study was to investigate the effectiveness and safety of extracorporeal venovenous cooling as compared with endovascular cooling.


METHODS
Swine were cooled in a randomized crossover design from 38 degrees C to 33 degrees C brain temperature, either with extracorporeal venovenous cooling or with endovascular cooling.


METHODS
Laboratory investigation.


METHODS
Six swine of human size (85 to 101 kg).


METHODS
Swine were randomly cooled with the first device, and after achieving the target brain temperature, re-warmed via the same technique and with heating lamps to baseline temperature. Then the other catheter was inserted and cooling was performed with the second device.


METHODS
Brain, pulmonary artery and tympanic temperature, blood pressure, and heart rate were recorded continuously. Laboratory samples, including free hemoglobin, were taken at predefined temperature points during cooling. Comparisons between and within (baseline vs. 33 degrees C) the treatment groups were performed with the paired Student's t-test.


RESULTS
The time needed to reduce brain temperature from 38.0 degrees C to 33.0 degrees C was 41 +/- 17 mins with venovenous cooling and 126 +/- 37 mins with endovascular cooling (p = .001). Heart rate and mean arterial pressure decreased moderately during cooling and were significantly lower at 33 degrees C than at baseline in both groups, without differences between groups. None of the swine developed significant hemolysis, arrhythmias, or bleeding.


CONCLUSIONS
Extracorporeal venovenous cooling was an effective and safe method to rapidly induce therapeutic mild hypothermia in human-sized swine. It seems to be promising for further application and investigation in patients.
Holzer
Michael
M
Department of Emergency Medicine, Medical University Vienna, Austria.
Behringer
Wilhelm
W
Janata
Andreas
A
Bayegan
Keywan
K
Schima
Heinrich
H
Deckert
Zeno
Z
Losert
Udo
U
Laggner
Anton N
AN
Sterz
Fritz
F
D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000502:physiology; D002545:Brain Ischemia / Q000628:therapy*; D002405:Catheterization, Central Venous / Q000379:methods; D018592:Cross-Over Studies; D005112:Extracorporeal Circulation; D005260:Female; D007036:Hypothermia, Induced / Q000379:methods*; D011897:Random Allocation; D018709:Statistics, Nonparametric; D013552:Swine; D013997:Time Factors