title: Polyphosphoinositides are derived from ether-linked inositol glycerophospholipids in rat brain.
abstract: Membrane inositol glycerophospholipid (IGP) is metabolized to phosphatidylinositol-4-phosphate (PIP), phosphatidylinositol-4, 5-bisphosphate (PIP2), and inositol triphosphate (IP3) in signaling transduction. This study was carried out to determine the subclasses of IGP involved in signaling pathway. The acyl chain moieties of the phospholipids are easily modulated by dietary fatty acids. We analyzed acyl chain composition of IGP 3-subclasses, PIP and PIP2 from rat brain after feeding sunflower seed oil enriched with linoleic acid or fish oil high in eicosapentaenoic acid and docosahexaenoic acid. Long chain polyunsaturated fatty acids (LCPUFA) as eicosapentaenoic acid and docosahexaenoic acid were not incorporated into ether-linked IGP (alkenylacylglycerophosphoinositol and alkylacyl-glycerophosphoinositol), PIP and PIP2, while diacyl-glycerophosphoinositol (GPI) contained high LCPUFA. These results suggest that PIP might be phosphorylated from only the ether-linked IGP (alkenylacyl- and alkylacyl species) but not from diacyl subclass for signals to intracellular responses in the plasma membrane of rat brain.
authors.affiliation: Lipid Chemistry Laboratory, Kyungpook National University, Daegu, 702-701 Korea.
authors.forename: Sun H
authors.initials: SH
authors.lastname: Shin
authors.forename: Jong S
authors.initials: JS
authors.lastname: Kim
authors.forename: Hak R
authors.initials: HR
authors.lastname: Kim
authors.forename: Jin K
authors.initials: JK
authors.lastname: Lim
authors.forename: Byung K
authors.initials: BK
authors.lastname: Choi
authors.forename: Young K
authors.initials: YK
authors.lastname: Yeo
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry; D004986:Ether; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D020404:Glycerophospholipids / Q000737:chemistry* / Q000302:isolation & purification; D006368:Helianthus; D007294:Inositol; D010716:Phosphatidylinositols / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D020404:Glycerophospholipids; D010716:Phosphatidylinositols; D004986:Ether; D007294:Inositol