[Expression of MLRQ subunit gene of NADH oxidoreductase and its clinical significance in malignant tumors of digestive system].
OBJECTIVE
To study the differential expression and possible role of MLRQ subunit gene of nicotinamide adenine dinucleotide reduced (NADH) oxidoreductase in malignant tumors of digestive system.


METHODS
Specimens of cancerous tissues and matched adjacent normal tissues resected during operation or biopsy: 38 pairs of specimens of esophageal carcinoma, 7 pairs of specimens of cardiac carcinoma, 14 pairs of specimens of gastric carcinoma, 11 pairs of specimens of colon carcinoma, and 7 pairs of specimens of liver carcinoma underwent PCR test and Northern hybridization to detect the differential expression of MLRQ subunit gene of NADH oxidoreductase.


RESULTS
(1) Overexpression of MLRQ subunit gene was found in 31 of the 38 pairs of specimens of esophageal carcinoma (81.6%), 4 of the 7 pairs of specimens of cardiac carcinoma (57.1%), 12 of the 14 pairs of specimens of gastric carcinoma (85.7%), 4 of the 7 pairs of specimens of colon carcinoma (65.6%), and 7 of the 11 pairs of specimens of liver cancer (57.1%). No significant difference among different cancers was observed by X(2) test (all P > 0.05). (2) The up-regulation of MLRQ subunit was not correlated with clinic stage, infiltration degree, lymphatic metastasis, and differentiation of tumor (all P > 0.05).


CONCLUSIONS
MLRQ subunit gene is up-regulated in the malignant tumors of digestive system.
Wang
Tao
T
Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China.
Liu
Zhi-hua
ZH
Li
Ze-jian
ZJ
Zhang
Li-yong
LY
Liu
Yu
Y
Sun
Guo-bin
GB
Wang
Xiu-qin
XQ
Wu
Min
M
D004067:Digestive System Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation
D009247:NADH, NADPH Oxidoreductases