title: [Study of the genetic stability of expression plasmid vector pMG36e in host bacteria].
abstract: OBJECTIVE
  To study the genetic stability of expression plasmid vector pMG36e in Escherichia coli JM109 and Lactococcus lactis MG1363/36e and observe the effect of erythomycin for its stability.
  
  
  METHODS
  We used classical method to determine the genetic stable rates of pMG36e in E. coli JM109 and Lactococcus lactis MG1363/36e with or without erythomycin selective pressure.
  
  
  RESULTS
  When E. coli JM109 were continuously incubated to 120 generations, its pMG36e genetic stable rates were 100% and 96% respectively with or without erythomycin selective pressure. When Lactococcus lactis MG1363/36e were continuously incubated to 100 generations, its pMG36e genetic stable rates were the same 98%, with or without erythomycin selective pressure.
  
  
  CONCLUSIONS
  pMG36e had good genetic stability in this two hosts. Erythromycin selective pressure had no significant effect for the genetic stability of pMG36e.
authors.affiliation: Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.forename: Chao-wu
authors.initials: CW
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Du
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Lü
mesh_terms: D003470:Culture Media; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D012692:Serial Passage
chemical_list: D003470:Culture Media; D004917:Erythromycin