Generation of landomycin D-producing strain Streptomyces globisporus LD3.
Rhodinosyl transferase gene lndGT4, governing the conversion of the disaccharide oligoketide ('polyketide') landomycin D into a trisaccharide derivative landomycin E, was deleted in Streptomyces globisporus 1912 genome. Possible resistance mechanisms that protect the resulting landomycin D-producing mutant strain S. globisporus LD3 against the toxic action of landomycins were determined.
Ostash
B
B
Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 79005 Lviv, Ukraine.
Kozarevska
I
I
Fedorenko
V
V
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006031:Glycosylation; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014166:Transferases / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C493499:landomycin D; C113593:landomycin E; D014166:Transferases