title: Local protein synthesis mediates a rapid increase in dendritic elongation factor 1A after induction of late long-term potentiation.
abstract: The maintenance of long-term potentiation (LTP) requires a brief period of accelerated protein synthesis soon after synaptic stimulation, suggesting that an early phase of enhanced translation contributes to stable LTP. The mechanism regulating protein synthesis and the location and identities of mRNAs translated are not well understood. Here, we show in acute brain slices that the induction of protein synthesis-dependent hippocampal LTP increases the expression of elongation factor 1A (eEF1A), the mRNA of which contains a 5' terminal oligopyrimidine tract. This effect is blocked by rapamycin, indicating that the increase in EF1A expression is mediated by the mammalian target of rapamycin (mTOR) pathway. We find that mRNA for eEF1A is present in pyramidal cell dendrites and that the LTP-associated increase in eEF1A expression was intact in dendrites that had been severed from their cell bodies before stimulation. eEF1A levels increased within 5 min after stimulation in a translation-dependent manner, and this effect remained stable for 3 h. These results suggest a mechanism whereby synaptic stimulation, by signaling through the mTOR pathway, produces an increase in dendritic translational capacity that contributes to LTP maintenance.
authors.affiliation: Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: Panayiotis
authors.initials: P
authors.lastname: Tsokas
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Grace
authors.forename: Pokman
authors.initials: P
authors.lastname: Chan
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Sealfon
authors.forename: Ravi
authors.initials: R
authors.lastname: Iyengar
authors.forename: Emmanuel M
authors.initials: EM
authors.lastname: Landau
authors.forename: Robert D
authors.initials: RD
authors.lastname: Blitzer
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003712:Dendrites / Q000502:physiology*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases
chemical_list: D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger; D011494:Protein Kinases; D058570:TOR Serine-Threonine Kinases