C-type lectin OCILRP2/Clr-g and its ligand NKRP1f costimulate T cell proliferation and IL-2 production.
We are reporting the identification of a novel C-type lectin receptor-ligand pair that is involved in T cell costimulation. The receptor, OCILRP2/Clr-g, is rapidly induced following T cell activation and maintained at a substantial level of up to 72 h. The ligand, NKRP1f, is predominantly expressed on dendritic cells (DC). The soluble OCILRP2-Ig blocking protein significantly suppresses specific antigen-stimulated T cell proliferation as well as IL-2 secretion both in vitro and in vivo; conversely, NKRP1f-expressing antigen presenting cells (APC) enhance B7.1/CD28-mediated costimulation for T cell proliferation through interaction with OCILRP2/Clr-g. Our studies reveal a unique functional interaction between two C-type lectins, OCILRP2/Clr-g and NKRP1f, during APC-mediated T cell costimulation and suggest a role for C-type lectins in maintaining T cell response or memory in vivo.
Tian
Wenzhi
W
Division of Immunology, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA.
Nunez
Rafael
R
Cheng
Shuhua
S
Ding
Yumei
Y
Tumang
Joseph
J
Lyddane
Clay
C
Roman
Christopher
C
Liou
Hsiou-Chi
HC
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors
C470572:Dcl1 protein, mouse; D007376:Interleukin-2; D037181:Lectins, C-Type; D008024:Ligands; D008565:Membrane Proteins; C442138:Nkrp1f protein, mouse; D020033:Protein Isoforms; D011971:Receptors, Immunologic