title: Absence of human papillomavirus infection in minimal deviation adenocarcinoma and lobular endocervical glandular hyperplasia.
abstract: The human papillomavirus (HPV) is basically always detected in squamous cell carcinoma of the cervix and its precursors; a high incidence of HPV also has been reported in adenocarcinoma and adenocarcinoma in situ of the uterine cervix. Lobular endocervical glandular hyperplasia (LEGH) was first described by Nucci in 1999. It is difficult to differentiate minimal deviation adenocarcinoma (MDA) from LEGH preoperatively or postoperatively by clinical and pathologic features. The relationships between HPV and MDA or LEGH have not been studied well because of the rare incidence of the two diseases. To our knowledge, the HPV status in LEGH has not been reported. This study was designed to investigate HPV infection in MDA and LEGH, using the polymerase chain reaction (PCR) technique. Tumor tissue lesions were microdissected and the detection of HPV and its typing were analyzed by PCR-based assay. As the control, HPV DNA was detected in all cases of squamous cell carcinoma and three of five cases of adenocarcinoma. However, no HPV DNA was detected in any of the 10 cases of LEGH or in the 3 cases of MDA. These results suggest that MDA and LEGH are probably not related to HPV infection.
authors.affiliation: Department of Obstetrics and Gynecology, University of Yamanashi, Yamanashi 409-3898, Japan.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Xu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kondo
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yuminamochi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nara
authors.forename: Keiko
authors.initials: K
authors.lastname: Hashi
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Murata
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D000368:Aged; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000254:growth & development*; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D014412:Tumor Virus Infections / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral