[Chemical induction -- new strategy of oocyte enucleation].
Mammalian somatic cloning has got great progress during past few years,however,the efficiency of nuclear trasfer remains low. In order to improve this technique, different perspectives of which are studying. Accordingly, the method of oocyte enucleation also becomes a focus. But the physical enucleation is technically demanding, time-consuming, inherently invasive and clearly damaging to cytoplast spatial organization. As a alternative strategy to traditional method, Demecolcine-induced enucleation(IE) attracted the scientist's eyes. Nuclear transfer procedure assited with IE, factors releated with success rate of IE and effects of IE on the oocytes and cloned embryos are mainly discussed in this review. At the same time, combining with author's research, the existing problems of IE and potential improved measures of some key steps in IE procedure are also elucidated. However, the utility of the demecolcine-induced enucleation protocol will require further deep investigation.
Wang
Qiang
Q
Institute of Bio-Engineering Northwest Sci- Tech University of Agriculture and Forestry, Yangling 712100, China. wangqiang7864@yahoo.com.cn
Gu
Ling
L
Zhang
Yong
Y
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D003703:Demecolcine / Q000494:pharmacology*; D006801:Humans; D008962:Models, Theoretical; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects*
D003703:Demecolcine