New method for DNA microarrays development: applied to human platelet antigens polymorphisms.
DNA microarrays are a powerful experimental tool for the detection of specific genomic sequences and are invaluable to a broad array of applications: clinical diagnosis, personalized medicine, drug research and development, gene therapy, food control technologies, and environmental sciences. Alloimmunization to human platelet antigens (HPAs) is commonly responsible for neonatal alloimmune thrombocytopenia, post-transfusional purpura and platelet transfusion refractoriness. Using DNA microarrays, we developed a diagnosis to type the biallelic HPA-1 platelet group. The region for the human genomic DNA sequence that contains the polymorphism responsible for HPA-1 alleles was amplified by polymerase chain reaction (PCR). The expected DNA fragments were hybridized on DNA microarrays, and the data were analyzed using specially developed software. Our initial results show that the two HPA-1 antigens polymorphisms containing a single base difference were detected using DNA microarrays.
Brès
J-C
JC
LEOM-UMR 5512, Ecole Centrale de Lyon, BP 163, 69131 Ecully Cedex, France.
Mérieux
Y
Y
Dugas
V
V
Broutin
J
J
Vnuk
E
E
Jaber
M
M
Rigal
D
D
Martin
J-R
JR
Souteyrand
E
E
Cabrera
M
M
Cloarec
J-P
JP
D016824:Antigens, Human Platelet / Q000097:blood* / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods; D039661:Integrin beta3; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3