Inhibitory effect of Gamibojungikgitang extract on mast cell-mediated allergic reaction in murine model.
OBJECTIVE
Gamibojungikgitang (GBIT) is an Oriental herbal prescription medication, which has been commonly used to treat allergic rhinitis in far Eastern countries including Korea, China, and Japan. Additionally, GBIT effectively treats ovarian cysts and improves ovarian functions by regulating both endocrine and metabolic activities. However, it has not been cleared how it prevents allergic diseases in experimental model. Here we report the effect of GBIT on mast cell-mediated allergic reactions.


METHODS
The anti-anaphylactic effect of GBIT extract was studied against compound 48/80-induced systemic anaphylactic shock model in mice. Rat Peritoneal Mast Cells (RPMCs) were used to investigate the effect of GBIT extract on histamine release induced by compound 48/80. Passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE was used to know the effect of GBIT extract. In addition, human mast cell line HMC-1 cells culture supernatants that GBIT extract pretreated were assayed for IL-6 protein levels by enzyme-linked immunosorbent assay method.


RESULTS
GBIT extract dose dependently inhibited compound 48/80-induced systemic anaphylactic shock. When GBIT extract was given as pretreatment at concentrations ranging 0.01-1 mg/ml, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. GBIT extract also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE. In addition, GBIT extract inhibited phorbol 12-myristate 13-acetate + A23187-induced interleukin-6 secretion from human mast cell line HMC-1 cells.


CONCLUSIONS
These results suggest a potential role for GBIT extract as a source of anti-anaphylactic agents for allergic disorders.
Moon
Phil-Dong
PD
College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, South Korea.
Na
Jo-Jeong
JJ
Jeong
Hyun-Ja
HJ
Hong
Seung-Heon
SH
Kim
Su-Jin
SJ
Chae
Han-Jung
HJ
Kim
Hyung-Ryong
HR
Choi
Jeong-On
JO
Lee
Si-Hyeong
SH
Shin
Jo-Young
JY
Kim
Hyung-Min
HM
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018514:Plant Structures; D051381:Rats; D017208:Rats, Wistar
D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal