Coherent phase microscopy in cell biology: visualization of metabolic states.
Visualization of functional properties of individual cells and intracellular organelles still remains an experimental challenge in cell biology. The coherent phase microscopy (CPM) provides a convenient and non-invasive tool for imaging cells and intracellular organelles. In this work, we report results of statistical analysis of CPM images of cyanobacterial cells (Synechocystis sp. PCC 6803) and spores (Bacillus licheniformis). It has been shown that CPM images of cyanobacterial cells and spores are sensitive to variations of their metabolic states. We found a correlation between one of optical parameters of the CPM image ('phase thicknesses' Deltah) and cell energization. It was demonstrated that the phase thickness Deltah decreased after cell treatment with the uncoupler CCCP or inhibitors of electron transport (KCN or DCMU). Statistical analysis of distributions of parameter Deltah and cell diameter d demonstrated that a decrease in the phase thickness Deltah could not be attributed entirely to a decrease in geometrical sizes of cells. This finding demonstrates that the CPM technique may be a convenient tool for fast and non-invasive diagnosis of metabolic states of individual cells and intracellular organelles.
Tychinsky
Vladimir P
VP
Moscow State Institute for Radioengineering, Electronics and Automation, prosp. Vernadskogo 78, 119454 Moscow, Russia.
Kretushev
Alexander V
AV
Vyshenskaya
Tatyana V
TV
Tikhonov
Alexander N
AN
D001407:Bacillus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003627:Data Interpretation, Statistical; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D055095:Optics and Photonics; D011190:Potassium Cyanide / Q000494:pharmacology; D013171:Spores, Bacterial / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D046939:Synechocystis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014475:Uncoupling Agents / Q000494:pharmacology
D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D004237:Diuron; D011190:Potassium Cyanide