title: In cat four times as many lamina I neurons project to the parabrachial nuclei and twice as many to the periaqueductal gray as to the thalamus.
abstract: The spinothalamic tract, and especially its fibers originating in lamina I, is the best known pathway for transmission of nociceptive information. On the other hand, different studies have suggested that more lamina I cells project to the parabrachial nuclei (PBN) and periaqueductal gray (PAG) than to the thalamus. The exact ratio of the number of lamina I projections to PBN, PAG and thalamus is not known, because comprehensive studies examining these three projections from all spinal segments, using the same tracers and counting methods, do not exist. In the present study, the differences in number and distribution of retrogradely labeled lamina I cells in each segment of the cat spinal cord (C1-Coc2) were determined after large wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) injections in either PBN, PAG or thalamus. We estimate that approximately 6000 lamina I cells project to PBN, 3000 to PAG and less than 1500 to the thalamus. Of the lamina I cells projecting to thalamus or PAG more than 80%, and of the lamina I-PBN cells approximately 60%, were located on the contralateral side. In all cases, most labeled lamina I cells were found in the upper two cervical segments and in the cervical and lumbar enlargements.
authors.affiliation: Department of Anatomy and Embryology, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, Building 3215, P.O. Box 196, 9700 AD Groningen, The Netherlands.
authors.forename: E M
authors.initials: EM
authors.lastname: Klop
authors.forename: L J
authors.initials: LJ
authors.lastname: Mouton
authors.forename: R
authors.initials: R
authors.lastname: Hulsebosch
authors.forename: J
authors.initials: J
authors.lastname: Boers
authors.forename: G
authors.initials: G
authors.lastname: Holstege
mesh_terms: D000818:Animals; D002415:Cats; D002452:Cell Count / Q000379:methods; D005260:Female; D007839:Functional Laterality / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010487:Periaqueductal Gray / Q000502:physiology*; D011149:Pons / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology; D013133:Spinothalamic Tracts / Q000166:cytology / Q000502:physiology; D013788:Thalamus / Q000502:physiology*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
chemical_list: D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate