title: Cytokeratin immunoreactivity patterns in short-segment Barrett's esophagus in Japanese patients.
abstract: BACKGROUND
  The origin of intestinal metaplasia at the esophagogastric junction has clinical importance. However, it can be difficult to differentiate between intestinal metaplasia of short-segment Barrett's esophagus and cardiac intestinal metaplasia due to Helicobacter pylori infection. Specific patterns of cytokeratin (CK)7 and CK20 have been detected in long-segment Barrett's esophagus. The aim of the present study was to assess the immunostaining patterns associated with short-segment Barrett's esophagus.
  
  
  OBJECTIVE
  Paraffin-embedded biopsy specimens were prepared from 128 patients with intestinal metaplasia of long-segment Barrett's esophagus (n = 3), short-segment Barrett's esophagus without H. pylori infection (n = 22), short-segment Barrett's esophagus with H. pylori infection (n = 22), and cardiac mucosa (n = 49) and gastric mucosa from antrum and fundus (n = 44) with H. pylori infection. Sections were prepared and immunostained for CK7 and CK20.
  
  
  RESULTS
  A Barrett's CK7/20 pattern was present in all three patients (100%) with long-segment Barrett's esophagus, 21 of 22 patients (95%) with short-segment Barrett's esophagus without H. pylori infection, and six of 22 patients (27%) with short-segment Barrett's esophagus with H. pylori infection (P < 0.05).
  
  
  CONCLUSIONS
  Intestinal metaplasia of short-segment Barrett's esophagus in patients without H. pylori infection is thought to be similar to that seen in long-segment Barrett's esophagus.
authors.affiliation: Department of Internal Medicine, Niigata Prefectural Yoshida Hospital, Yoshida-machi, Nishikanbara-gun, Niigata 959-0242, Japan. yagikazu@pop12.odn.ne.jp
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yagi
authors.forename: Atsuo
authors.initials: A
authors.lastname: Nakamura
authors.forename: Atsuo
authors.initials: A
authors.lastname: Sekine
mesh_terms: D000328:Adult; D000368:Aged; D001471:Barrett Esophagus / Q000453:epidemiology / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D018450:Disease Progression; D016099:Endoscopy, Gastrointestinal; D004947:Esophagus / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007564:Japan / Q000453:epidemiology; D053554:Keratin-20; D053552:Keratin-7; D007633:Keratins / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; C507499:KRT20 protein, human; C507497:KRT7 protein, human; D053554:Keratin-20; D053552:Keratin-7; D007633:Keratins