[Fluorometric method for determination of keratin SH-groups in human epidermis].
Simple and highly sensitive fluorometric method for determination of keratin SH-groups using fluorescein mercuric acetate has been developed. It allows to determine amount of SH-groups up to 0.1 nmole/mg of protein in extracts of corneal layer of human epidermis.
Morenkova
S A
SA
Nagler
L G
LG
D004817:Epidermis / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007633:Keratins / Q000737:chemistry*; D009941:Organomercury Compounds / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis*
D005452:Fluoresceins; D009941:Organomercury Compounds; D013438:Sulfhydryl Compounds; C001673:fluorescein mercuric acetate; D007633:Keratins