title: Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics.
abstract: BACKGROUND
  The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.
  
  
  RESULTS
  Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.
  
  
  CONCLUSIONS
  The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.
authors.affiliation: Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA. wange@umbi.umd.edu
authors.forename: Wenming
authors.initials: W
authors.lastname: Wang
authors.forename: Milos
authors.initials: M
authors.lastname: Tanurdzic
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
authors.forename: Nicholas
authors.initials: N
authors.lastname: Sisneros
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: Jing-Ke
authors.initials: JK
authors.lastname: Weng
authors.forename: Dave
authors.initials: D
authors.lastname: Kudrna
authors.forename: Christopher
authors.initials: C
authors.lastname: Mueller
authors.forename: K
authors.initials: K
authors.lastname: Arumuganathan
authors.forename: John
authors.initials: J
authors.lastname: Carlson
authors.forename: Clint
authors.initials: C
authors.lastname: Chapple
authors.forename: Claude
authors.initials: C
authors.lastname: de Pamphilis
authors.forename: Dina
authors.initials: D
authors.lastname: Mandoli
authors.forename: Jeff
authors.initials: J
authors.lastname: Tomkins
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
authors.forename: Jo Ann
authors.initials: JA
authors.lastname: Banks
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D005434:Flow Cytometry; D015723:Gene Library; D018745:Genome, Plant; D023281:Genomics; D009693:Nucleic Acid Hybridization; D032503:Selaginellaceae / Q000235:genetics*