Genomic DNA microarrays for Entamoeba histolytica: applications for use in expression profiling and strain genotyping.
The parasite Entamoeba histolytica is a causative agent of dysentery and liver abscesses. Found predominantly in developing countries, this parasitic infection is responsible for significant morbidity and mortality. We have developed a genomic DNA microarray for E. histolytica. The array composed of 11,328 clones contains >2000 unique genes and was utilized for expression profiling and comparative genomic hybridizations of Entamoeba strains. We present a synopsis of our results to date and potential future applications of microarray technology for the study of Entamoeba biology.
MacFarlane
Ryan
R
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5124, USA.
Bhattacharya
Dhruva
D
Singh
Upinder
U
D000818:Animals; D016172:DNA Fingerprinting; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000469:parasitology*; D020869:Gene Expression Profiling / Q000379:methods*; D018503:Genome, Protozoan; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*