title: Luminal nitric oxide in ileal reservoirs for continent cutaneous diversion or orthotopic bladder reconstruction.
abstract: OBJECTIVE
  To measure mucosal inflammation as reflected in nitric oxide (NO) production in ileal reservoirs for the storage of urine and to correlate it with the growth of bacteria as well as CRP.
  
  
  METHODS
  Intraluminal gas NO concentrations were determined using the chemoluminescence technique in 25 patients with continent cutaneous ileal reservoirs (Kock pouch) and 12 patients with orthotopic bladders (hemi-Kock or T-pouch). NO concentrations were determined in both intestinal reservoir gas and silicon catheter balloon gas. Urinary culture and blood CRP determinations were performed.
  
  
  RESULTS
  NO concentrations in reservoir gas were higher than in silicon catheter balloons. Bacteriuria was associated with approximately 20 times higher NO concentrations than sterile urine. NO concentrations did not differ between continent cutaneous reservoirs or orthotopic bladders when due attention was paid to variance in the rate of bacteriuria. Elevated CRP was associated with higher NO concentrations. Bacteriuria with acinetobacter, enterococci and pseudomonas appeared to cause comparatively lower NO concentrations. The inflammatory response of reservoir walls to bacteriuria did not decrease with time.
  
  
  CONCLUSIONS
  Urine in itself causes much less intestinal wall inflammation than bacteriuria, as reflected in NO production. High CRP values are associated with high NO concentrations. The inflammatory response varies with the bacterial specimens.
authors.affiliation: Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Pazooki
authors.forename: Anders
authors.initials: A
authors.lastname: Kilander
authors.forename: Elisabet
authors.initials: E
authors.lastname: Lindholm
authors.forename: Anna-Carin
authors.initials: AC
authors.lastname: Olin
authors.forename: Harriet
authors.initials: H
authors.lastname: Törnqvist
authors.forename: Kjell-Arne
authors.initials: KA
authors.lastname: Ung
authors.forename: Olof
authors.initials: O
authors.lastname: Jonsson
mesh_terms: D000388:Air / Q000032:analysis*; D001419:Bacteria / Q000302:isolation & purification; D001437:Bacteriuria / Q000097:blood / Q000382:microbiology; D002097:C-Reactive Protein / Q000378:metabolism; D039021:Colonic Pouches / Q000502:physiology*; D015169:Colony Count, Microbial; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D001745:Urinary Bladder Diseases / Q000601:surgery; D016476:Urinary Reservoirs, Continent
chemical_list: D009569:Nitric Oxide; D002097:C-Reactive Protein