[Effect of 2-methoxyestradiol on cell differentiation of myeloma cell line CZ-1].
OBJECTIVE
To investigate the differentiation induction effect of 2-methoxyestradiol (2ME2), an estrogen derivative on myeloma cell line CZ-1.


METHODS
The changes of CZ-1 cells in morphology, expression of surface CD49e and quantity of light chain secretion in the supernatant were observed when treated with 0.1 approximately 0.5 micromol/L 2ME2 for 48 h.


RESULTS
2ME2 could induce differentiation of CZ-1 cells. The cells appeared decreased in size of nucleus, increased in cytoplasma, decreased in the ratio of nucleus to plasma, decreased in number or disappearance of nucleolus, and thickness and pyknosis of chromatin. The expression of CD49e was increased from (12.20 +/- 1.57)% to (24.80 +/- 1.26)% (P < 0.05). Light chain secretion in the supernatant was increased from (35.97 +/- 2.60) microg/ml to (79.67 +/- 1.88) microg/ml (P < 0.05).


CONCLUSIONS
Low concentrations of 2ME2 could induce differentiation of myeloma cell line CZ-1.
Gao
Wei-Ran
WR
Department of Hematology, Changzheng Hospital of Second Military Medical University, Shanghai 200003, China.
Hou
Jian
J
D000077584:2-Methoxyestradiol; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D039482:Integrin alpha5 / Q000032:analysis; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology; D050257:Tubulin Modulators / Q000494:pharmacology
D039482:Integrin alpha5; D050257:Tubulin Modulators; D004958:Estradiol; D000077584:2-Methoxyestradiol