[Polymorphisms within the interferon-Gamma receptor associated with systemic lupus erythematosus].
OBJECTIVE
To assess the association between polymorphisms within the interferon-Gamma receptor gene(IFN-GammaR) and systemic lupus erythematosus (SLE) in Chinese people.


METHODS
The IFN-GammaR genotypes of 94 SLE patients and 80 healthy subjects were examined by the reverse transcription-polymerase chain reaction (RT-PCR)-single-strand conformation polymorphism (SSCP) method, RT-PCR-restriction fragment length polymorphism (RFLP) method and DNA sequencing.


RESULTS
There was no statistically significant difference in the IFN-GammaR genotype frequencies between the two groups. The IFN-Gamma R2 Arg64/Arg64 genotype decreased the risk of SLE (OR = 2.481, 95% CI 0.992 - 6.203, P = 0.047). The decreased risk of the development of SLE was detected in the individuals who had the combination of IFN-GammaR2 Arg64/Arg64 genotype and IFN-GammaR1 Val14/Val14 genotype (OR = 2.481, 95% CI 0.992 - 6.203, P = 0.047).


CONCLUSIONS
The IFN-GammaR2 Arg64/Arg64 genotype does not determine susceptibility to SLE in Chinese people, and the combination of IFN-Gamma R2 Arg64/Arg64 genotype and IFN-Gamma R1 Val14/Val14 genotype does not, either.
Yao
Xu
X
Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing, Jiangsu, 210042, PR China.
Li
Yu-mei
YM
Hu
Zi-jia
ZJ
Li
Wen-zhong
WZ
Chen
Zhi-qiang
ZQ
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D017471:Receptors, Interferon / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D000096743:Interferon gamma Receptor
D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor