title: Decreased phosphatidylethanolamine binding protein expression correlates with Abeta accumulation in the Tg2576 mouse model of Alzheimer's disease.
abstract: Phosphatidylethanolamine binding protein (PEBP) is a multifunctional protein, with proposed roles as the precursor protein of hippocampal cholinergic neurostimulating peptide (HCNP), and as the Raf kinase inhibitor protein (RKIP). Previous studies have demonstrated a decrease in PEBP mRNA in CA1 region of AD hippocampus. The current study demonstrates that PEBP is decreased in the hippocampus of 11 month Tg2576 mice, in the absence of change in mRNA levels compared to non-transgenic littermates. The level of PEBP in transgenic mouse hippocampus significantly decreases at 11 months (a time point when Abeta begins accumulating) and 15 months (when Abeta plaques have formed). There was a significant correlation between decreased PEBP expression and accumulation of Abeta. Immunohistochemical studies on Tg2576 and AD brain sections demonstrate that PEBP immunoreactivities are present at the periphery of dense multicore Abeta plaques, and in selective astrocytes, primarily surrounding plaques. These findings suggest that PEBP expression may be influenced by accumulation of Abeta. Down-regulation of PEBP may result in lower levels of HCNP or altered coordination of signal transduction pathways that may contribute to neuronal dysfunction and pathogenesis in AD.
authors.affiliation: Department of Pathology, University of Melbourne, Parkville 3010, Australia.
authors.forename: Amee J
authors.initials: AJ
authors.lastname: George
authors.forename: R M Damian
authors.initials: RM
authors.lastname: Holsinger
authors.forename: Catriona A
authors.initials: CA
authors.lastname: McLean
authors.forename: Seong-Seng
authors.initials: SS
authors.lastname: Tan
authors.forename: Hamish S
authors.initials: HS
authors.lastname: Scott
authors.forename: Tina
authors.initials: T
authors.lastname: Cardamone
authors.forename: Roberto
authors.initials: R
authors.lastname: Cappai
authors.forename: Colin L
authors.initials: CL
authors.lastname: Masters
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Li
mesh_terms: D000367:Age Factors; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D049488:Phosphatidylethanolamine Binding Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D058225:Plaque, Amyloid / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D016229:Amyloid beta-Peptides; D049488:Phosphatidylethanolamine Binding Protein; D012333:RNA, Messenger