title: PCR-TGGE: a method for fingerprinting the microbial flora in the small intestine of pigs.
abstract: The bacterial flora of the small intestine is still not fully understood. This is also due to the fact that a lot of these gut inhabitants aren't cultivable. PCR-TGGE (Temperature Gradient Gel Electrophoresis) is a molecular tool by which the 16S ribosomal DNA of a bacterial community can be studied for its diversity. Based on sequence differences in the variable region V6-V8, 16S ribosomal DNA fragments are separated during a TGGE run. This study shows that PCR-TGGE is a valuable tool as it gives additional information about the flora composition that could not be found with classical bacteriological techniques.
authors.affiliation: University of Ghent, Department of Animal Production, Proefhoevestraat 10, 9090 Melle, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Van den Bossche
authors.forename: C
authors.initials: C
authors.lastname: Van Nevel
authors.forename: L
authors.initials: L
authors.lastname: Herman
authors.forename: J
authors.initials: J
authors.lastname: Decuypere
authors.forename: S
authors.initials: S
authors.lastname: De Smet
authors.forename: N
authors.initials: N
authors.lastname: Dierick
authors.forename: M
authors.initials: M
authors.lastname: Heyndrickx
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary*; D007421:Intestine, Small / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial