title: Relaxin inhibits central angiotensin II expression in killifish: a central osmoregulatory role for relaxin and angiotensin II in the killifish Fundulus heteroclitus.
abstract: We investigated whether relaxin and brain angiotensin II (AII) are osmoregulatory hormones in killifish. Four groups of killifish acclimatized to different saline environments (freshwater, 25% sea water, 50% sea water [brackish], and sea water) were euthanized and their brains were embedded in paraffin. A separate group of brackish water killifish were injected with relaxin 1 h before dissection and embedding. Serial sagittal sections were immunohistochemically stained for AII. Angiotensin II immunostaining did not differ between saline environments. Contrary to expectation, relaxin decreased the number of AII-immunoreactive cells in hypothalami of brackish water fish.
authors.affiliation: Department of Biology, Acadia University, Wolfville, NS, Canada B4P 2R6.
authors.forename: Adwoa
authors.initials: A
authors.lastname: Amamoo
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D023781:Fundulidae / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D012065:Relaxin / Q000378:metabolism / Q000494:pharmacology*; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D000804:Angiotensin II; D012065:Relaxin