[The function of ERK on Fas-mediated apoptotic signaling pathway of human gastric carcinoma cells].
OBJECTIVE
To analyze the expression and functions of ERK (extracellular regulated kinase) in Fas-mediated apoptosis in gastric carcinoma cell line SGC-7901 and to elucidate the potential significance of this signaling pathway in tumor progression.


METHODS
Radioisotope labeling and Western blotting with special anti-ERK antibody were used to check ERK activity in SGC-7901 cell line after anti-Fas antibody treatment. Apoptosis induced by several treatment factors was evaluated by FACS can flow cytometer.


RESULTS
ERK activity increased and reached the peak at 30 min after treatment with anti-Fas antibody and decreased in PD98059 pretreated group. The number of sub-G(1) cell was 30.5% +/- 2.6% in PD98059 pretreated group, which was higher than anti-Fas treatment group and control group, respectively.


CONCLUSIONS
In gastric cancer cell line SGC-7901, Fas-induced ERK activation may suppress Fas-mediated apoptosis. Inhibition of ERK may enhance the sensitivity of SGC-7901 cells to Fas-mediated apoptosis. Fas-induced ERK activation may confer gastric cancer cells ability to escape the immune surveillance.
Liu
Ning
N
Division of Biochemistry & Molecular Biology, Shenyang Medical College, Shenyang 110034, China.
Sun
Li-guang
LG
Yu
Hui-ying
HY
Zhang
Yi
Y
Yang
Qing
Q
Wang
Xiao-peng
XP
D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000378:metabolism*; D006801:Humans; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000473:pathology*
D053222:Fas Ligand Protein; D048049:Extracellular Signal-Regulated MAP Kinases