title: [Identification of the related gene of mouse tolerant to exposure to perfluoroisobutylene by DDRT-PCR].
abstract: OBJECTIVE
  The mice surviving acute exposure to low dose of perfluoroisobutylene (PFIB) could tolerant to lethal dose of PFIB. Discovering the gene related with the tolerance would be significant to find preventive agents for PFIB.
  
  
  METHODS
  By static exposure to PFIB for 3 times, the survival animals could tolerate to lethal dose of PFIB. Three anchoring primers and 4 arbitrary primers were applied in differential display reverse transcription PCR (DDRT-PCR) of lung tissues and the products were analyzed by sequencing PAGE and silver staining. The differential DNA fragments were cloned and sequenced.
  
  
  RESULTS
  There were obvious differences of gene expression between control and tolerant animals. Three expressed sequence tags (EST) have been cloned and sequenced,which were homologous to some mouse gene in Genbank.
  
  
  CONCLUSIONS
  Specific gene up-regulation might be the cause of tolerance to PFIB, and they are very important to clarity the tolerant mechanisms to PFIB.
authors.affiliation: Nanjing Military Medical Institute, Nanjing 210002, China.
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Tang
authors.forename: Shu
authors.initials: S
authors.lastname: Rong
authors.forename: Shu-biao
authors.initials: SB
authors.lastname: Ding
mesh_terms: D000818:Animals; D004361:Drug Tolerance / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D005466:Fluorocarbons / Q000506:poisoning*; D015870:Gene Expression; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D005466:Fluorocarbons; D012333:RNA, Messenger; C016648:perfluoroisobutylene