A high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives anti-trichomonas activity.
Several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. A fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. The colorimetric technique was based on the use of Alamar blue as a redox-indicator. The test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determination of 50% and 90% inhibitory concentrations), the latter took advantage of the dye fluorometric properties. The performance of the method was excellent affording an exactitude 97.86% and a reproducibility of 95% and no interference of the trichomonas-culture medium was observed during the test. Some of the nitroimidazole compounds tested showed a fair trichomonicidal activity, however none of them was as active as the model compound, metronidazole.
Campos Aldrete
Ma Elena
ME
Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas-IPN, Prol. Carpio y Plan de Ayala, S/N CP 11340 México, D.F., Mexico. camesol22@hotmail.com
Salgado-Zamora
Héctor
H
Luna
Julieta
J
Meléndez
Estela
E
Meráz-Ríos
Marco A
MA
D000818:Animals; D000994:Antitrichomonal Agents / Q000633:toxicity*; D001681:Biological Assay; D003124:Colorimetry; D005470:Fluorometry; D007202:Indicators and Reagents; D009593:Nitroimidazoles / Q000633:toxicity*; D010078:Oxazines; D010084:Oxidation-Reduction; D014246:Trichomonas vaginalis / Q000187:drug effects*; D014966:Xanthenes
D000994:Antitrichomonal Agents; D007202:Indicators and Reagents; D009593:Nitroimidazoles; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin