The DNA-binding specificity of the Bacillus anthracis AbrB protein.
The Bacillus subtilis AbrB protein is a DNA-binding global regulator of a plethora of functions that are expressed during the transition from exponential growth to stationary phase and under suboptimal growth conditions. AbrB orthologues have been identified in a variety of prokaryotic organisms, notably in all species of Bacillus, Clostridium and Listeria that have been examined. Based on amino acid sequence identity in the N-terminal domains of the orthologues from B. subtilis and Bacillus anthracis, it was predicted that the proteins might display identical DNA-binding specificities. The binding of purified B. anthracis AbrB (AbrB(BA)) and purified B. subtilis AbrB (AbrB(BS)) at DNA targets of B. subtilis, B. anthracis and a synthetic origin was compared. In all cases examined, DNA-binding specificity was identical as judged by DNase I footprinting. In B. subtilis cells, the B. anthracis promoters from the atxA and abrB genes were regulated by AbrB(BS), and the B. subtilis promoter from the yxbB operon was regulated by AbrB(BA).
Strauch
Mark A
MA
Department of Biomedical Sciences, Dental School, University of Maryland, Baltimore, 666 W. Baltimore Street, Baltimore, MD 21201, USA.
Ballar
Petek
P
Molecular and Cell Biology Program, University of Maryland, Baltimore, 108 N. Greene St, Baltimore, MD 21201, USA.
Rowshan
Austin J
AJ
Department of Biomedical Sciences, Dental School, University of Maryland, Baltimore, 666 W. Baltimore Street, Baltimore, MD 21201, USA.
Zoller
Katherine L
KL
Department of Biomedical Sciences, Dental School, University of Maryland, Baltimore, 666 W. Baltimore Street, Baltimore, MD 21201, USA.
D001408:Bacillus anthracis / Q000502:physiology*; D001412:Bacillus subtilis / Q000502:physiology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D013379:Substrate Specificity; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*
C060762:AbrB protein, Bacillus subtilis; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors