Synthesis, loading, and application of individual nanocapsules for probing single-cell signaling.
This paper describes the synthesis and loading of silica and polystyrene-acrylic based nanocapsules with small molecules. The nanocapsules are used for delivering defined packages of stimuli to single cells with both high spatial and temporal resolutions. To introduce molecules into the capsules, we characterized two approaches. The first approach is based on a base-swell process in which the shell of the capsule is swelled so small molecules can diffuse into the interior of the capsule and be trapped inside once the capsules are de-swelled. The second approach is based on a dry-swell-dry process in which the solution containing the molecules of interest and the nanocapsules is physically dried to promote more molecules to enter into the interior of the capsule. We characterized both methods by monitoring the content of and the release from individual capsules with confocal microscopy and wide-field imaging. To illustrate the biological applications of such nanocapsules, we used optical trapping to position a single carbachol-loaded capsule adjacent to a single CHO cell that has been transfected with muscarinic acetylcholine (M1) receptors, released the carbachol from the capsule with a single 3-ns N2 laser pulse, and then monitored the subsequent intracellular signaling triggered by the binding of carbachol to the M1 receptors.
Sun
Bingyun
B
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
Chiu
Daniel T
DT
D000818:Animals; D016466:CHO Cells; D002214:Capsules / Q000138:chemical synthesis* / Q000737:chemistry; D002217:Carbachol / Q000378:metabolism / Q000494:pharmacology; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D003102:Colloids; D006224:Cricetinae; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D011976:Receptors, Muscarinic / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry
D002214:Capsules; D003102:Colloids; D011976:Receptors, Muscarinic; D012834:Silver; D012822:Silicon Dioxide; D002217:Carbachol