In vitro differentiation of human dental follicle cells with dexamethasone and insulin.
The dental follicle is an ectomesenchymally derived connective tissue harboring precursor cells for the tooth supporting apparatus. In this study, we examined gene expression of freshly isolated human dental follicle cells during osteogenic differentiation in vitro. These plastic adherent fibroblastic cells express Notch-1, nestin and vimentin. We differentiated dental follicle cells with dexamethasone or insulin-based protocols into membrane-like structures containing mineralizing foci. An analysis of mineralized tissue with atomic force microscopy illustrated a bone and cementum-like structure. A real-time RT-PCR analysis was developed to investigate expression of typical osteoblast or cementoblast related genes during differentiation. Gene expressions of osteocalcin (OCN), bone morphogenic protein (BMP)-2 and nestin were increased during the both differentiation approaches. Our work demonstrates differentiation of dental follicle cells with an insulin-based protocol for the first time.
Morsczeck
C
C
Stiftung caesar, Center of Advanced European Studies and Research, Ludwig Erhard Allee 2, 53175 Bonn, Germany. morsczeck@gmx.de
Moehl
C
C
Götz
W
W
Heredia
A
A
Schäffer
T E
TE
Eckstein
N
N
Sippel
C
C
Hoffmann
K H
KH
D000328:Adult; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D003739:Dental Cementum; D003795:Dental Sac / Q000187:drug effects* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D010012:Osteogenesis; D010641:Phenotype; D051881:Receptor, Notch1 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D007328:Insulin; D007381:Intermediate Filament Proteins; C577484:NES protein, human; C497761:NOTCH1 protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D051881:Receptor, Notch1; D016212:Transforming Growth Factor beta; D003907:Dexamethasone