title: Hepatosplenic gammadelta T-cell lymphoma.
abstract: OBJECTIVE
  To investigate the clinicopathologic characteristics, immunophenotype and TCR gene rearrangements of hepatosplenic T-cell lymphoma in eight Chinese patients.
  
  
  METHODS
  Eight Chinese patients with hepatosplenic gammadelta T-cell lymphomas were studied. Hematoxylin-eosin-stained slides and clinical histories were reviewed. We also carried out immunohistochemical staining for CD3, CD4, CD8, CD20, CD43, CD56, CD79a, UCHL-1, and TCR gammadelta. Rearrangements of TCR gamma and delta chain genes were also studied.
  
  
  RESULTS
  The spleens were enlarged and the cut surfaces were homogeneous and red-purple in color without identifiable gross lesions or enlarged hilar lymph nodes. Histologically, lymphoma cells infiltrated the cords of Billroth and often packed the sinuses. Liver biopsy showed lymphoma cell infiltrations in the sinusoids, and three cases showed involvements of the portal tracts. Immunohistochemically lymphoma cells were positive for CD3, CD43, and CD56 in all cases. Four of eight cases were positive for CD8, and all cases were negative for CD4 (6/6). Monoclonal rearrangements of TCR gamma gene were demonstrated by PCR analysis in five out of the eight cases. TCR delta gene rearrangements were detected in six out of the eight cases, which demonstrated single bands on PAGE gel, and the amplification products in two cases were confirmed by sequencing.
  
  
  CONCLUSIONS
  The clinicopathology of hepatosplenic gammadelta T-cell lymphoma in Chinese patients is similar to what was previously reported except that the splenomegaly is not so massive, and CD8 is positive.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Shuan-Zeng
authors.initials: SZ
authors.lastname: Wei
authors.forename: Tong-Hua
authors.initials: TH
authors.lastname: Liu
authors.forename: De-Tian
authors.initials: DT
authors.lastname: Wang
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Cao
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liang
mesh_terms: D000328:Adult; D001483:Base Sequence; D002648:Child; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D006529:Hepatomegaly / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000276:immunology; D013160:Splenic Neoplasms / Q000276:immunology* / Q000473:pathology; D013163:Splenomegaly / Q000473:pathology
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta