title: Influence of vedaprofen (Quadrisol) on quality and freezability of stallion semen.
abstract: The objective of this study was to evaluate the effect of the non-steroidal anti-inflammatory drug (NSAID) vedaprofen (Quadrisol) on quality and freezability of stallion semen. Experiments were performed using 22 Franches Montagnes stallions from the National Stud in Avenches (Switzerland) randomly divided into a control and test group. Vedaprofen was given orally to all stallions of the test group at the recommended therapeutic dose (initial dose of 2mg/kg followed by 1mg/kg body weight every 12h) for 14 days. Control animals received the same amount of carrier substance. During treatment, blood samples of five stallions in both test and control group were collected for PGF(2 alpha)-metabolite (PG-metabolite) determination. Ejaculates from all stallions were collected and cryopreserved weekly for 14 weeks from September to December. Concentrations of PG-metabolite, PGF and PGE were measured in the seminal plasma of ejaculates collected 2 weeks before, during and 2 weeks after treatment. In fresh semen the volume, concentration, motility and number of normal sperm and sperm with major defects (acrosome defects, abnormal heads, nuclear vacuoles, proximal droplets, midpiece defects) were evaluated. In frozen-thawed semen samples motility as well as viability (SYBR-14/PI) were tested and the hypoosmotic swelling test (HOS) was performed. Results demonstrate that vedaprofen had no effect on blood plasma concentration of PG-metabolite but significantly inhibited both, PGF and PGE concentrations in seminal plasma. Furthermore, all quality parameters in fresh and frozen-thawed semen were not affected by vedaprofen treatment but the time of semen collection had a significant (P<0.05) effect on motility, normal sperm and sperm with nuclear vacuoles in fresh semen.
authors.affiliation: Clinic of Reproduction, University of Zürich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland. fjanett@vetclinics.unizh.ch
authors.forename: F
authors.initials: F
authors.lastname: Janett
authors.forename: L
authors.initials: L
authors.lastname: Aebi
authors.forename: D
authors.initials: D
authors.lastname: Burger
authors.forename: I
authors.initials: I
authors.lastname: Imboden
authors.forename: M
authors.initials: M
authors.lastname: Hässig
authors.forename: H
authors.initials: H
authors.lastname: Kindahl
authors.forename: R
authors.initials: R
authors.lastname: Thun
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002467:Cell Nucleus / Q000648:ultrastructure; D015925:Cryopreservation / Q000662:veterinary*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D006736:Horses; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology*; D011422:Propionates / Q000494:pharmacology*; D011458:Prostaglandins E / Q000032:analysis; D011460:Prostaglandins F / Q000032:analysis; D011786:Quality Control; D012621:Seasons; D012661:Semen / Q000737:chemistry / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000648:ultrastructure; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009281:Naphthalenes; D011422:Propionates; D011458:Prostaglandins E; D011460:Prostaglandins F; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D015237:Dinoprost; C121784:2-(4-cyclohexyl-1-naphthalenyl)propanoic acid