Tissue microarrays: fast-tracking protein expression at the cellular level.
Tissue microarrays maximize returns in cellular pathology whilst minimizing the use of cells and tissues. They are made by arraying cores of tissue taken from multiple donor blocks into a single recipient block. Accordingly, the histology and pathology of several hundred tissues can be represented in one tissue microarray that, when stained by immunohistochemistry, provides comprehensive topographic information on protein expression. Used with complimentary techniques, such as complementary DNA microarray analysis, tissue microarrays are providing valuable data for the identification of new markers of disease and assisting in the discovery of therapeutic targets. They are also leading a revolution in cellular pathology as high-throughput technology is introduced to maximize the information provided.
Warford
Anthony
A
Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. aw1@sanger.ac.uk
D000818:Animals; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods
D011506:Proteins