Fabrication of thromboresistant multilayer thin film on plasma treated poly (vinyl chloride) surface.
Layer-by-layer deposited anticoagulant multilayer films were prepared on ammonia plasma treated poly (vinyl chloride) (PVC). Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) and contact angle results revealed the presence of -NH2 on the ammonia plasma treated PVC surfaces and the layer-by-layer self-assembly process. The stability of multilayer film was studied with the radio labeled method. The remainder bovine serum albumin (BSA) in cross-linked 5(heparin/BSA) multilayer films dipped in phosphate buffered saline (PBS, pH 7.4) was more than 90% in 40 days. The static platelet adhesion result indicated the anticoagulant multilayer films deposited on the plasma treated PVC reduced platelet adhesion drastically and no thrombus forming. The plasma recalcification time revealed that the multilayer modified surfaces greatly prolonged the plasma recalcification time. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices.
Tan
Qinggang
Q
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China.
Ji
Jian
J
Zhao
Feng
F
Fan
De-Zeng
DZ
Sun
Fu-Yu
FY
Shen
Jia-Cong
JC
D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005740:Gases / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011143:Polyvinyl Chloride / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry*; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control*
D020099:Coated Materials, Biocompatible; D005740:Gases; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D011143:Polyvinyl Chloride