title: Expression of feline interferon-alpha subtypes in Esherichia coli, and their antiviral activity and animal species specificity.
abstract: Two kinds of FeIFN-alpha consisting of 166 amino acids (aa) and 171 aa were expressed in Escherichia coli, and the purified proteins were tested for antiviral activity on homologous and heterologous animal cells. Crude FeIFN induced in feline cells revealed antiviral activity on both homologous and heterologous animal cells. In contrast, both types of recombinant FeIFN-alpha revealed antiviral activity only on the feline cells. All of the FeIFN-alpha subtypes showed high activity to vesicular stomatitis virus, and the three species of feline viruses belonging to different families.
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, USA.
authors.forename: Osamu
authors.initials: O
authors.lastname: Taira
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Aramaki
authors.forename: Itsuki
authors.initials: I
authors.lastname: Sakurai
authors.forename: Takao
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kenji
authors.initials: K
authors.lastname: Motokawa
authors.forename: Setsuo
authors.initials: S
authors.lastname: Arai
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Sato
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Maehara
mesh_terms: D000818:Animals; D017927:Calicivirus, Feline / Q000276:immunology; D002415:Cats / Q000276:immunology* / Q000821:virology; D004926:Escherichia coli; D016766:Feline Infectious Peritonitis / Q000276:immunology; D015870:Gene Expression; D006564:Herpesviridae / Q000276:immunology; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000502:physiology*; D030781:Organisms, Genetically Modified; D013045:Species Specificity; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014780:Viruses / Q000276:immunology*
chemical_list: D016898:Interferon-alpha