title: Suppressive effect of deoxynivalenol, a Fusarium mycotoxin, on bovine and porcine neutrophil chemiluminescence: an in vitro study.
abstract: We evaluated the immunotoxicity of deoxynivalenol (DON), a Fusarium mycotoxin, on bovine and porcine neutrophils in vitro by using two function parameters, luminol-dependent chemiluminescence and random migration under agarose. A 2-hr DON treatment suppressed the chemiluminescence of bovine and porcine cells by 42% and 35% (on average) at 10(-5)M, and by 19% and 26% at 10(-6)M. Slight suppression was observed at concentrations lower than 10(-6)M. However, after an 18-hr DON treatment, random migration of neutrophils of both species remained unaffected, even at the highest concentration (10(-5)M). Although further extensive studies are needed, to our knowledge this is the first study to have revealed in vitro that DON can affect neutrophil function.
authors.affiliation: Department of Safety Research, National Institute of Animal Health, 3-1-5 Kannon-dai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hideko
authors.initials: H
authors.lastname: Takayama
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimada
authors.forename: Osamu
authors.initials: O
authors.lastname: Mikami
authors.forename: Hideo
authors.initials: H
authors.lastname: Murata
mesh_terms: D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D013552:Swine; D014255:Trichothecenes / Q000494:pharmacology*
chemical_list: D014255:Trichothecenes; D008165:Luminol; C007262:deoxynivalenol