Replaceable cross-linked polyacrylamide for high performance separation of proteins.
An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matrix generated high resolutions for a wide range (approximately 4 kD to approximately 300 kD) of proteins. When compared to the most frequently used sieving matrixes, the rCPA permitted the highest resolutions with comparable or increased separation speed for protein separations.
Lu
Joann J
JJ
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA.
Liu
Shaorong
S
Pu
Qiaosheng
Q
D000180:Acrylic Resins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000592:standards; D005782:Gels / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013997:Time Factors; D000099166:Polyacrylamides
D000180:Acrylic Resins; D005782:Gels; D011506:Proteins; D000099166:Polyacrylamides