title: AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro.
abstract: Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10(-1) to 10(-5) M) incubated for 21 days on aseptic tissue culture media WPM containing 1 microM Cu. Zn2+ caused phytotoxicity only at high concentrations (10(-1) to 10(-2) M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.
authors.affiliation: St. Stephanus University, Department of Genetics and PB, Gödöllö, Hungary. gyulai.gabor@mkk.szie.hu
authors.forename: Gábor
authors.initials: G
authors.lastname: Gyulai
authors.forename: Mervyn
authors.initials: M
authors.lastname: Humphreys
authors.forename: András
authors.initials: A
authors.lastname: Bittsánszky
authors.forename: Kirsten
authors.initials: K
authors.lastname: Skøt
authors.forename: József
authors.initials: J
authors.lastname: Kiss
authors.forename: Leif
authors.initials: L
authors.lastname: Skøtc
authors.forename: Gábor
authors.initials: G
authors.lastname: Gullner
authors.forename: Sue
authors.initials: S
authors.lastname: Heywood
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.forename: Alan
authors.initials: A
authors.lastname: Lovatt
authors.forename: László
authors.initials: L
authors.lastname: Radimszky
authors.forename: Hywel
authors.initials: H
authors.lastname: Roderick
authors.forename: Heinz
authors.initials: H
authors.lastname: Rennenberg
authors.forename: Michael
authors.initials: M
authors.lastname: Abberton
authors.forename: Tamás
authors.initials: T
authors.lastname: Komíves
authors.forename: László
authors.initials: L
authors.lastname: Heszky
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D003300:Copper / Q000493:pharmacokinetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D018515:Plant Leaves; D030821:Plants, Genetically Modified / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D032107:Populus / Q000378:metabolism*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D003300:Copper