Simultaneous quantification of different cyclodextrins and Gantrez by HPLC with evaporative light scattering detection.
A rapid and simple HPLC method with evaporative scattering detection (ELSD) has been developed for the separation and quantitation of beta-cyclodextrin (beta-CD), 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) and poly(methyl vinyl ether-co-maleic anhydride) (Gantrez). Separation was carried out on a Zorbax Eclipse XDB-Phenyl narrow bore column, with water-acetonitrile in gradient elution as mobile phase at a flow-rate of 0.25 ml/min. Polyethylenglycol 6000 was used as internal standard. The limit of quantification was of about 0.2 mg/ml for cyclodextrins and 0.05 mg/ml for Gantrez. The precision did not exceed 7%. This method was successfully applied to the rapid analysis of CD-Gantrez nanoparticle conjugates without interference from other components of the formulation.
Agüeros
M
M
Centro Galénico, Universidad de Navarra, Aptdo. 177, C/ Irunlarrea s/n, 31080 Pamplona, Navarra, Spain.
Campanero
M A
MA
lrache
J M
JM
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002138:Calibration; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D002942:Circular Dichroism; D003505:Cyclodextrins / Q000737:chemistry*; D008027:Light; D049329:Nanostructures; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D011092:Polyethylene Glycols