title: Antibody reactivity to linear epitopes of Plasmodium falciparum cytoadherence-linked asexual gene 9 in asymptomatic children and adults from papua new Guinea.
abstract: The cytoadherence-linked asexual gene 9 (clag 9) of Plasmodium falciparum has been implicated in the cytoadherence of infected erythrocytes. To determine the immunogenicity of the clag 9 gene product (CLAG 9 protein) in humans, we measured antibody responses to 11 synthetic CLAG 9 peptides in a group of 177 asymptomatic children and adults subject to intense malaria exposure in Madang, Papua New Guinea. The CLAG 9 peptides were immunogenic in adults and children. Antibody responses to peptides 4 and 10 were high across all age groups and detectable in a majority of children less than five years of age. While CLAG 9 peptides are immunogenic in humans, longitudinal studies will be required to determine the longevity of antibody responses to CLAG 9 and their role in protection from disease.
authors.affiliation: Division of Infectious Diseases and Immunology, The Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, Brisbane, Queensland, Australia. kathT@qimr.edu.au
authors.forename: Katharine R
authors.initials: KR
authors.lastname: Trenholme
authors.forename: Craig S
authors.initials: CS
authors.lastname: Boutlis
authors.forename: Rachel
authors.initials: R
authors.lastname: Kuns
authors.forename: Moses
authors.initials: M
authors.lastname: Lagog
authors.forename: Moses J
authors.initials: MJ
authors.lastname: Bockarie
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Gatton
authors.forename: David J
authors.initials: DJ
authors.lastname: Kemp
authors.forename: Michael F
authors.initials: MF
authors.lastname: Good
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Anstey
authors.forename: Donald L
authors.initials: DL
authors.lastname: Gardiner
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D002448:Cell Adhesion / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D002648:Child; D017125:Genes, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D009512:New Guinea / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D000913:Antibodies, Protozoan; D015815:Cell Adhesion Molecules; D015800:Protozoan Proteins; C407823:clag9 protein, Plasmodium falciparum