No association of the 94T/G polymorphism in the adiponectin gene with diabetic complications.
OBJECTIVE
This study examined a possible association of the T/G polymorphism at nucleotide 94 in the adiponectin gene with the prevalence of diabetic complications.


METHODS
The study was performed in 696 patients with type 1 diabetes and type 2 diabetes. Genotyping was performed by means of polymerase chain reaction and subsequent cleavage by using SmaI restriction endonuclease.


RESULTS
The 94G/G genotype was significantly more prevalent in patients with type 2 diabetes (2.2%) than in type 1 diabetics (0.0%) (p = 0.02), whereas no differences were found for frequencies of the 94T/T and the 94G/T genotypes, respectively. In patients with type 1 diabetes, 45 of 239 patients were heterozygous for the 94T/G polymorphism (carrier rate (CR): 18.8%; allele frequency (AF): 0.094). In type 2 diabetics, 71 of 457 patients were heterozygous and 10 patients were homozygous for the 94G/G genotype (CR: 17.7%; AF: 0.10). No association with diabetic nephropathy, diabetic neuropathy or diabetic retinopathy was found for either genotype in patients with type 1 and type 2 diabetes.


CONCLUSIONS
The 94T/G polymorphism in the adiponectin gene is not associated with diabetic complications. The significance of a higher prevalence of the G allele in type 2, compared to type 1 diabetes remains to be clarified.
Rudofsky
G
G
Division of Endocrinology and Metabolism, Department of Medicine, University of Heidelberg, Heidelberg, Germany.
Schlimme
M
M
Schlotterer
A
A
von Eynatten
M
M
Reismann
P
P
Tafel
J
J
Grafe
I
I
Morcos
M
M
Nawroth
P
P
Bierhaus
A
A
Hamann
A
A
D000328:Adult; D000483:Alleles; D003430:Cross-Sectional Studies; D048909:Diabetes Complications / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D003928:Diabetic Nephropathies / Q000235:genetics; D003929:Diabetic Neuropathies / Q000235:genetics; D003930:Diabetic Retinopathy / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011110:Polymorphism, Genetic / Q000235:genetics*