Radiation therapy induced modulation of wound healing at experimental vein graft anastomoses.
OBJECTIVE
The aim of this study was to investigate if radiation therapy (RT) favorably modulates wound healing at vein graft anastomoses.


METHODS
Jugular vein grafts were sewn into carotid arteries in 32 rats which were randomly divided into two groups: RT (gamma source, 14 Gray, n=16) and control (C, sham irradiation, n=16). Grafts and adjacent arteries were analyzed at 2 (n=8) and 8 weeks (n=8) by histology, immunohistochemistry, and morphometry.


RESULTS
Although, RT did not reduce the overall occurrence of intimal hyperplasia, the distribution differed. RT led to a reduction of intimal hyperplasia in arterial segments (median: C: 41.873 microm2; RT: 6.452 microm2, p < 0.0007). In contrast, RT augmented intimal hyperplasia in vein grafts (median: C: 30.287 microm2; RT: 90.455 microm2, p < 0.014). Vein graft diameters after RT were enlarged (median: C: 2.098 microm; RT: 3.381, p < 0.031). Over 80% of the cells were of mesenchymal origin in both groups.


CONCLUSIONS
RT reduced intimal hyperplasia in arterial segments. However, RT led to graft dilatation and increased intimal hyperplasia in vein grafts. RT did not favorably modulate the vascular wound healing response in this model.
Heckenkamp
J
J
Division of Vascular Surgery, Department of Vascular and Visceral Surgery, University of Cologne, Cologne, Germany. joerg.heckenkamp@uk-koeln.de
Lieder
K
K
Lang
E
E
Aleksic
M
M
Bendel
M S
MS
Gawenda
M
M
Fries
J W U
JW
Brunkwall
J S
JS
D000714:Anastomosis, Surgical; D000818:Animals; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014680:Veins / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery* / Q000637:transplantation; D014945:Wound Healing / Q000528:radiation effects*