Protein microarrays as a discovery tool for studying protein-protein interactions.
Exploring the function of the genome and the encoded proteins has emerged as a new and exciting challenge in the postgenomic era. Novel technologies come into view that promise to be valuable for the investigation not only of single proteins, but of entire protein networks. Protein microarrays are the innovative assay platform for highly parallel in vitro studies of protein-protein interactions. Due to their flexibility and multiplexing capacity, protein microarrays benefit basic research, diagnosis and biomedicine. This review provides an overview on the basic principles of protein microarrays and their potential to multiplex protein-protein interaction studies.
Korf
Ulrike
U
Division of Molecular Genome Analysis (B050), DKFZ Heidelberg, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. U.Korf@DKFZ.de
Wiemann
Stefan
S
D004261:DNA Replication; D006801:Humans; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011494:Protein Kinases / Q000032:analysis; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D020798:Two-Hybrid System Techniques
D043562:Receptors, G-Protein-Coupled; D016159:Tumor Suppressor Protein p53; D011494:Protein Kinases