Albumin peptide: a molecular marker for trauma/hemorrhagic-shock in rat mesenteric lymph.
Vascular permeability and endothelial cell damage has been shown to occur in rats subjected to trauma with hemorrhagic-shock. Although the factors responsible for the endothelial cell injury are unknown, it has been hypothesized that toxic factors produced in response to hemorrhagic-shock originate in the gut and are absorbed into the mesenteric lymphatics. Consistent with this hypothesis, it has been shown that lymph collected from animals subjected to trauma with hemorrhagic-shock (T/HS) results in a marked decrease in endothelial cell viability both in vitro and in vivo. We therefore compared the lymph collected pre-T/HS to samples collected during, and up to 3h post-T/HS in order to identify a factor present or increased in post-T/HS lymph. This analysis revealed that a single cationic peptide band was significantly increased in post-T/HS lymph, but not in lymph from control animals subjected to trauma without hemorrhagic-shock (T/SS). This peptide was subsequently identified as the N-terminal 24 amino acids of rat serum albumin (RSA) by mass spectrometry and amino acid sequencing. Although the measured increase in the albumin peptide correlates with detectable shock lymph-induced endothelial cell toxicity, the peptide was not toxic to endothelial cells. We therefore propose that the significant increase in the albumin peptide is a marker for post-T/HS lymph-induced endothelial cell toxicity.
Kaiser
Vicki L
VL
Department of Surgery, UMD-New Jersey Medical School, P.O. Box 1709, Newark, NJ 07101, USA. kaiser@umdnj.edu
Sifri
Ziad C
ZC
Senthil
Maheswari
M
Dikdan
George S
GS
Lu
Qi
Q
Xu
Da-Zhong
DZ
Deitch
Edwin A
EA
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002199:Capillary Permeability; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis*; D012771:Shock, Hemorrhagic / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D014947:Wounds and Injuries / Q000150:complications / Q000378:metabolism* / Q000473:pathology
D015415:Biomarkers; D010455:Peptides; D012709:Serum Albumin