PTEN deletion in Bergmann glia leads to premature differentiation and affects laminar organization.
Development of the central nervous system is controlled by both intrinsic and extrinsic signals that guide neuronal migration to form laminae. Although defects in neuronal mobility have been well documented as a mechanism for abnormal laminar formation, the role of radial glia, which provide the environmental cues, in modulating neuronal migration is less clear. We provide evidence that loss of PTEN in Bergmann glia leads to premature differentiation of this crucial cell population and subsequently to extensive layering defects. Accordingly, severe granule neuron migration defects and abnormal laminar formation are observed. These results uncover an unexpected role for PTEN in regulating Bergmann glia differentiation, as well as the importance of time-dependent Bergmann glia differentiation during cerebellar development.
Yue
Qing
Q
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Groszer
Matthias
M
Gil
Jose S
JS
Berk
Arnold J
AJ
Messing
Albee
A
Wu
Hong
H
Liu
Xin
X
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D017353:Gene Deletion; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D051059:PTEN Phosphohydrolase; D017027:Protein Tyrosine Phosphatases / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology
D025521:Tumor Suppressor Proteins; D017027:Protein Tyrosine Phosphatases; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse