Gene expression profile in oral squamous cell carcinoma: a pilot study.
OBJECTIVE
To study the gene expression profile of oral squamous cell carcinomas.


METHODS
Gene expression profile was investigated in oral squamous cell carcinomas in 5 patients using the Atlas Glass Human 3.8 I Microarray (which detects cDNA obtained from cellular total RNA) (Clontech Laboratories, Palo Alto, CA). Data were normalized by the LOWESS method. Statistical significances of deviations from a 1:1 ratio were evaluated by t tests, with P<.05.


RESULTS
Of the 3,757 genes analyzed, 322 (8.6%) were significantly overexpressed in tumoral tissue with respect to normal tissue, while 104 (2.8%) were significantly underexpressed. The affected genes fell into a wide range of functional categories.


CONCLUSIONS
We consider that cDNA microarrays are of clear value for investigating the biology of these tumors, and that this technology may help in the molecular classification of oral squamous cell carcinomas and in the identification of targets for gene therapy.
Somoza-Martín
José Manuel
JM
Universidad de Santiago de Compostela, Complejo Hospitalario, Universitario de Santiago, Spain.
García-García
Abel
A
Barros-Angueira
Francisco
F
Otero-Rey
Eva
E
Torres-Español
María
M
Gándara-Vila
Pilar
P
Reboiras-López
María Dolores
MD
Blanco-Carrión
Andrés
A
Gándara-Rey
José Manuel
JM
D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects
D004273:DNA, Neoplasm