title: Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid.
abstract: BACKGROUND
  Trinitrobenzene sulphonic acid (TNBS) induces chronic transmural inflammatory lesions in the rat colon. Injury is facilitated by barrier disruption and invasion of commensal bacteria. However, certain bacteria have shown anti-inflammatory properties in in vitro models.
  
  
  OBJECTIVE
  To investigate in vivo the anti-inflammatory effect of Lactobacillus casei DN-114 001.
  
  
  METHODS
  Rats with a colonic segment excluded from faecal transit were surgically prepared. After washing the lumen with antibiotics, the excluded segment was recolonized (control group: standard flora of rat origin; test group: standard flora and L casei). Microbial colonisation was confirmed by culture of segment washing, and colitis was then induced by instillation of TNBS. One day after, intestinal lesions were blindly graded by macro- and microscopic scores, and myeloperoxidase activity measured in tissue homogenates. Translocation of bacteria to mesenteric lymph nodes, spleen and liver was investigated.
  
  
  RESULTS
  Test rats showed a smaller area of mucosal injury than control rats (p<0.05). Maximum depth lesion scores were similar in both groups but myeloperoxidase activity was lower in test than in control rats (p<0.05). Remarkably, bacterial translocation was quantitatively lower (p<0.01) and less frequent (p<0.05) in test than in control rats.
  
  
  CONCLUSIONS
  In rats colonised with L casei, mucosal injury, inflammatory response, and barrier disruption after TNBS challenge were attenuated. Bacterial communities colonising the mucosa can modify inflammatory responses to luminal challenges.
authors.affiliation: Digestive System Research Unit, Hospital General Vall d'Hebron, Barcelona 08035, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Llopis
authors.forename: M
authors.initials: M
authors.lastname: Antolín
authors.forename: F
authors.initials: F
authors.lastname: Guarner
authors.forename: A
authors.initials: A
authors.lastname: Salas
authors.forename: J-R
authors.initials: JR
authors.lastname: Malagelada
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000382:microbiology / Q000628:therapy*; D003106:Colon / Q000382:microbiology; D004195:Disease Models, Animal; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000382:microbiology*; D007780:Lacticaseibacillus casei / Q000502:physiology*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000382:microbiology; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase