title: [Study on the RNA of severe acute respiratory syndrome (SARS) associated coronavirus in the blood and excretion of convalescent patients with SARS].
abstract: OBJECTIVE
  To examine the RNA of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease.
  
  
  METHODS
  A total of 276 samples, including plasma, urine, feces and sputum, obtained from 23 convalescent patients with SARS were studied at 3 time-points at least 21 days after the onset of symptoms. RNA was extracted and nested reverse transcription-polymerase chain reaction (RT-PCR) was carried out using SARS-CoV specific primers.
  
  
  RESULTS
  Among the 276 samples, SARS-CoV RNA was detected in 6 cases (38.8%) by nested RT-PCR. The positive rates of SARS-CoV RNA was 5.8% in feces and 2.9% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases.
  
  
  CONCLUSIONS
  The existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the excretion from these patients should be carefully treated whilthe re-transmission of SARS by which, should be further studied.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Zhao-rui
authors.initials: ZR
authors.lastname: Chang
authors.forename: Ren-quan
authors.initials: RQ
authors.lastname: Yang
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Li-li
authors.initials: LL
authors.lastname: Ren
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yao-wu
authors.initials: YW
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Yan-gong
authors.initials: YG
authors.lastname: Chao
authors.forename: Cheng-yi
authors.initials: CY
authors.lastname: Qu
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003289:Convalescence; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000821:virology*; D013183:Sputum / Q000821:virology
chemical_list: D012367:RNA, Viral