A sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein A-V concentration.
Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106 patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V concentration was 179.2 +/- 74.8 ng/ml, and it was greater in females than in males (P < 0.005). It was correlated positively with the plasma HDL cholesterol (r = 0.32, P < 0.0001), apoA-I (r = 0.27, P = 0.0001), and apoE (r = 0.18, P = 0.011) concentrations and negatively with plasma TG concentration (r = -0.22, P = 0.021). In relation to single nucleotide polymorphism 3 (-1131C/T) of the apoA-V gene, apoA-V concentration was higher in the T/T type than in the C/C type (P < 0.01). Plasma TG concentration was lower in the T/T type than in the C/C or C/T type (P < 0.05). ApoA-V concentration was lower in the diabetic patients (69.4 +/- 44.3 ng/ml; P < 0.01) than in the healthy controls.
Ishihara
Mitsuaki
M
Department of Advanced Medical Technology and Development, BML, Inc., Saitama, Japan.
Kujiraoka
Takeshi
T
Iwasaki
Tadao
T
Nagano
Makoto
M
Takano
Mayumi
M
Ishii
Jun
J
Tsuji
Masahiro
M
Ide
Hajime
H
Miller
Irina P
IP
Miller
Norman E
NE
Hattori
Hiroaki
H
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I / Q000097:blood; D000072040:Apolipoprotein A-V; D001053:Apolipoproteins / Q000097:blood* / Q000235:genetics; D001054:Apolipoproteins A; D001057:Apolipoproteins E / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values; D012727:Sex Characteristics; D014280:Triglycerides / Q000097:blood
C437704:APOA5 protein, human; D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D000072040:Apolipoprotein A-V; D001053:Apolipoproteins; D001054:Apolipoproteins A; D001057:Apolipoproteins E; D008076:Cholesterol, HDL; D014280:Triglycerides