[Effects of nutrient conditions and fed-batch culture on CoQ10 production by Rhizobium radiobacter WSH2601].
The effects of the variables, including the concentrations of glucose, sucrose, corn steep liquor (CSL) and peptone, and the conditions of fed-batch culture, on CoQ10 fermentation by Rhizobium radiobacter WSH2601 were assessed. The results showed that the optimum concentrations of glucose, sucrose, CSL and peptone were 30 g/L, 40 g/L, 11 g/L and 16 g/L respectively. Addition of CSL and tomato juice stimulated the cell growth. CSL, L-methionine and isopentyl alcohol efficiently increased the biosynthesis of CoQ10. In a 7L fermentor, the fed-batch culture improved both cell growth and CoQ10 production compared to a batch culture; and suitable mixed feeding of CSL and sucrose enhanced CoQ10 yield to 52.4 mg/L. The DCW reached 26.4 g/L, an increase of 53% in comparison to that without feeding, and an increase of 24% to that feeding with sucrose only. The C/B value reached 2.38 mg/g(DCW), representing an increase of 33% to that of without feeding, and an increase of 26% to that of feeding with sucrose only.
Zu-Fan
Wu
W
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, China.
Du
Guo-Cheng
GC
Chen
Jian
J
D016960:Agrobacterium tumefaciens / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D013395:Sucrose / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D003470:Culture Media; D014451:Ubiquinone; D013395:Sucrose; C024989:coenzyme Q10; D005947:Glucose