title: Relaxin stimulates cAMP production in MCF-7 cells upon overexpression of type V adenylyl cyclase.
abstract: Relaxin stimulates cAMP production and activation of ERK and PI3K in THP-1 cells. Relaxin also stimulates protein kinase C zeta (PKCzeta) translocation to the plasma membrane in a PI3K-dependent manner in THP-1 and MCF-7 cells. However, relaxin did not increase cAMP production in MCF-7 cells. We overexpressed different adenylyl cyclase (AC) isoforms in MCF-7 cells to examine coupling of endogenous relaxin receptors to cAMP production. Overexpression of types II and IV AC had no effect on cAMP production by relaxin. However, overexpression of type V AC, which is activated by PKCzeta, showed synergistic stimulation of cAMP by relaxin and forskolin.
authors.affiliation: Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, TX 77030, USA.
authors.forename: Bao T
authors.initials: BT
authors.lastname: Nguyen
authors.forename: Carmen W
authors.initials: CW
authors.lastname: Dessauer
mesh_terms: D000262:Adenylyl Cyclases / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000096:biosynthesis*; D015870:Gene Expression; D006801:Humans; D012065:Relaxin / Q000494:pharmacology*
chemical_list: D012065:Relaxin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases