[Cytometric assessment of apoptosis and aneuploidy in relation to survival time in osteosarcomas of children and adolescents].
Apoptosis programmed cell death takes place in response to many different stimuli, which include DNA damage. This is interest for therapeutic and prognostic purposes in neoplasms. Laboratory studies have shown that many anti-cancer agents (including cytostatics) achieve their cytotoxic effect by inducing apoptosis. Flow cytometry methods have found application in identification and enumeration of apoptotic cells, which were used in the present study. The retrospective study concerning children and adolescents osteosarcomas has shown, that longer survival time correlated with higher apoptotic index (apoptotic cells in "pre-G1/G0" position of DNA histograms) in tumours after preoperative chemotherapy. Shorter survival time (up to 3 years) correlated with small apoptotic population and bigger aneuploid fraction. This suggests that response to applied chemotherapy of these osteosarcomas was relatively small. These preliminary results have shown, that estimation of the apoptotic population can be helpful in prognosis and effects of chemotherapy. It should be confirmed on fresh tumour material after preoperative chemotherapy and longer clinical observation.
Rytwiński
Krzysztof
K
Samodzielna Pracownia Cytometrii, Instytut Matki i Dziecka, Kasprzaka 17a, 01-211 Warszawa, Poland. cytom@pf.pl
Woźniak
Wojciech
W
Rychłowska
Magda
M
Wnuk
Aleksander
A
D000293:Adolescent; D000782:Aneuploidy; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000401:mortality* / Q000601:surgery; D017024:Chemotherapy, Adjuvant; D002648:Child; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000188:drug therapy / Q000401:mortality* / Q000601:surgery; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D016019:Survival Analysis; D016896:Treatment Outcome
D000970:Antineoplastic Agents