Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglioside antibody repertoire.
Neuropathogenic murine antibodies reactive with terminal disialylgalactose epitopes are innate and preferentially encoded by the VH7183.3b gene. Here we have studied antibodies reactive with internal galactose-linked disialosyl epitopes and the terminal trisaccharide of GT1b. Antibodies were of moderate affinity and unmutated. Anti-GD1b antibodies were often encoded by the VH10.2b heavy and gj38c light chain genes. Anti-GT1b antibodies with broader glycan binding patterns were encoded by VHQ52 and VHJ558 family genes. These data indicate that the discrete specificities of ganglioside-binding antibodies are dictated by particular patterns of V gene usage residing within the innate B cell repertoire.
Boffey
Judith
J
University Department of Neurology, Institute of Neurological Sciences, Southern General Hospital, Glasgow, Scotland G51 4TF, United Kingdom.
Odaka
Masaaki
M
Nicoll
Dawn
D
Wagner
Eric R
ER
Townson
Kate
K
Bowes
Tyrone
T
Conner
Joe
J
Furukawa
Koichi
K
Willison
Hugh J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000378:metabolism; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism; D005732:Gangliosides / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D012799:Sialyltransferases / Q000172:deficiency / Q000235:genetics; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D007135:Immunoglobulin Variable Region; C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase