[Effects of macrophage migration inhibitory factor on collagen I and III mRNA expression in vascular smooth muscle cells in vitro].
OBJECTIVE
To investigate the association between macrophage migration inhibitory factor (MIF) and collagen synthesis in vascular smooth muscle cells (VSMCs).


METHODS
VSMCs isolated from SD rat aorta were cultured in different culture plates, and the fourth passage of cells were divided into two groups, one of which was simulated by MIF and the other served as control group. Collagen I and III mRNA expression in the VSMCs was detected by reverse transcriptase (RT)-PCR and PCR.


RESULTS
Collagen I and III mRNA expression in MIF-treated VSMCs increased significantly as compared with that in the control cells (P<0.05).


CONCLUSIONS
MIF increases collagen I and III mRNA expression of VSMCs in vitro, indicating its involvement in collagen synthesis in VSMCs.
Li
Jun
J
Institute of Cardiovascular Diseases, Guangdong People's Hospital, Guangzhou 510080, China. linzhongxiangma@sina.com
Lin
Shu-guang
SG
Yu
Xi-yong
XY
Lin
Qiu-xiong
QX
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D024061:Collagen Type III; D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger