title: [Affection of metallothionein-3 to dUTPase's accommodating cellular toxicity of dUTP].
abstract: Metallothionein-3 (MT-3), renamed as growth inhibitory factor (GIF), is a brain specific member of the metallothionein family. Human dUTPase is a recently found protein in brain that can interact with hMT-3. They have the growth inhibitory activity on neuron cell by interaction. To study the affection of hMT-3 to dUTPase's eliminating the cellular toxicity caused by dUTP, the pSVHA-dUTPase and pFLag-hMT-3 genes have been transfected into HEK293 cells. In addition, the dUTPase and hMT-3 proteins were expressed in BL21 to study the role of hMT-3 on the hydrolyzation of dUTP by dUTPase. The results demonstrate that the cells co-transfected with dUTPase and hMT-3 genes have more strong resistibility to dUTP than the cells transfected only with dUTPase gene. And that the hMT-3 protein can accelerate the hydrolyzation of dUTP by dUTPase. All these indicate that hMT-3 can cooperate with dUTPase to protect better the 293 cells from dUTP. This research offered the theoretic elements for the application of hMT-3 and dUTPase in chemic cure.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, National Laboratory of Protein Engineering, Beijing 100871, China.
authors.forename: Qiao-Lin
authors.initials: QL
authors.lastname: Chen
authors.forename: Qiao-Hua
authors.initials: QH
authors.lastname: Kang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ren
authors.forename: Zong-Yuan
authors.initials: ZY
authors.lastname: Wang
authors.forename: Bing-Gen
authors.initials: BG
authors.lastname: Ru
mesh_terms: D002460:Cell Line; D003856:Deoxyuracil Nucleotides / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D054730:Protein Interaction Domains and Motifs; D011755:Pyrophosphatases / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D014162:Transfection
chemical_list: D003856:Deoxyuracil Nucleotides; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C027078:deoxyuridine triphosphate; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase