Interleukin-1beta induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP.
Interleukin-1 beta (IL-1) and CREB have many CNS actions including sleep regulation and hippocampal-dependent learning. CREB acts in part via CREB-binding protein (CBP). We thus determined whether IL-1 could induce CBP gene expression. Initially, cultured hippocampal cells were treated with IL-1 and differential display reverse transcription was used to identify up- and down-regulated genes. We then sequenced rat CBP. Of the IL-1-upregulated genes, CBP and adenine nucleotide translocator-1 (ANT-1) were investigated in vivo. In these experiments, IL-1 was given to rats intraventricularly and sacrificed 2 h later; both CBP and ANT-1 transcripts were upregulated in the cerebral cortex and hypothalamus. We conclude that rat CBP shares many of the functional domains as human and murine CBP and that IL-1 upregulates genes previously associated with learning and sleep.
Tang
Chad
C
Department of VCAPP, Program in Neuroscience, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA.
Sula
Mee Ja
MJ
Bohnet
Stewart
S
Rehman
Abdur
A
Taishi
Ping
P
Krueger
James M
JM
D033741:Adenine Nucleotide Translocator 1 / Q000235:genetics; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects / Q000235:genetics*; D050882:CREB-Binding Protein; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D017124:Conserved Sequence / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000378:metabolism* / Q000494:pharmacology; D007858:Learning / Q000502:physiology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012890:Sleep / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
D033741:Adenine Nucleotide Translocator 1; D007375:Interleukin-1; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C494364:Crebbp protein, mouse; C494365:Crebbp protein, rat