Synergistic effect of the combination triclosan with 2-phenylphenol against Pseudomonas aeruginosa and fungi.
OBJECTIVE
Triclosan is active against Gram-positive and Gram-negative bacteria but lesser against Pseudomonas aeruginosa (P.aeruginosa). 2-phenylphenol is considered as an effective agent against fungi but its antifungal action is more important than its antibacterial activity. The aim of this study is to evaluate the bactericidal and fungicidal activities of triclosan and 2-phenylphenol alone, and in combination against standard strains of bacteria and fungi.


METHODS
The study was carried out in the Research Laboratory of Pharmaceutical Microbiology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan, during the year 2004. The standard French method of in-vitro dilution quantitative suspension test, according to the Association Françoise de Normalization (AFNOR) guideline, has been used to determine the antibacterial and antifungal activity of each agent alone as well as the combination of the agents.


RESULTS
The antibacterial activity of the combination triclosan-2-phenylphenol was significantly enhanced over that of each agent used alone against P.aeruginosa. Synergistic effect was observed against all tested strains of fungi.


CONCLUSIONS
This synergistic effect that was exhibited by triclosan-2-phenylphenol combination can be considered to enhance the antibacterial activity against P.aeruginosa and the antifungal activity of the 2 agents, to reduce the in-use concentration of each agent used alone, that may minimize any possible side effect of the 2 agents, and to avoid the occurrence of bacterial resistance to one of the 2 agents.
Tumah
Haitham N
HN
Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, PO Box 3030, Irbid 22110, Jordan. tumah@just.edu.jo
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D004357:Drug Synergism; D005658:Fungi / Q000187:drug effects*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D066298:In Vitro Techniques; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014260:Triclosan / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D001713:Biphenyl Compounds; D005659:Fungicides, Industrial; D014260:Triclosan; C004369:2-phenylphenol