[Expression of P53 and Bax in rat liver induced by microcystin LR in vivo].
To examine the expression of P53, Bcl-2 and Bax proteins in rat liver after exposed to microcystin LR.



SD rats received microsystin LR by gastric perfusion. The expression of P53, Bax and Bcl-2 in liver was detected by Western blot.



The expression of P53 and Bax in each treatment group increased significantly compared with that in control group(P<0.05), with the exception of 0.1 microg/kg LR exposure group. Moreover, with exposure levels increasing the expression of P53 and Bax increased gradually; while no changes of the expression of Bcl-2 were observed.



P53 and Bax may play important roles in microcystin LR induced apoptosis, but Bcl-2 seems not be involved in this process.
Wang
Xiu-min
XM
Department of Biochemistry and Molecular Biology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Chen
Jia-ping
JP
Fu
Wen-yu
WY
Xu
Li-hong
LH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001427:Bacterial Toxins / Q000633:toxicity; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008387:Marine Toxins / Q000633:toxicity; D052998:Microcystins; D010456:Peptides, Cyclic / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics; D051028:bcl-2-Associated X Protein
D001427:Bacterial Toxins; C494828:Bax protein, rat; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C057862:cyanoginosin LR