title: Triacylglycerol structure and composition of hydrogenated soybean oil margarine and shortening basestocks.
abstract: The composition and structures of triacylglycerols (TAG) in a commercially prepared hydrogenated soybean oil margarine basestock [iodine value (IV) 65, 39.7% trans fatty acids] were determined by high-performance liquid chromatography (HPLC) in tandem with atmospheric pressure chemical ionization (APCI) mass spectrometry (MS). The basestock was separated by preparative HPLC into four fractions. Fractions 1 and 4, constituting approximately 8% of the total, were shown to consist of LOO, PLO, and LLS and OSS and PSS, respectively (where L = linoleic, O = oleic, S = stearic, and P = palmitic). APCI will not distinguish between O, oleic cis C18:1, and E, elaidic trans C18:1. Thus, O and E may be used interchangeably in discussion of TAG isomer structures. Fraction 2 consisted of OOO and POO. Fraction 3 consisted of OOO, POO, OOS, and POS. About 80% of the total triglycerides consisted of OOO, POO, and OOS. The trans fatty acid content of the fractions was determined, and the results showed that 92% of the total trans content was found in fractions 2 and 3. A shortening basestock (IV 81.7, 31.8% trans fatty acids) was partially characterized.
authors.affiliation: Food and Industrial Oil Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA. listGR@ncaur.usda.gov
authors.forename: Gary R
authors.initials: GR
authors.lastname: List
authors.forename: William C
authors.initials: WC
authors.lastname: Byrdwell
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Steidley
authors.forename: Richard O
authors.initials: RO
authors.lastname: Adlof
authors.forename: William E
authors.initials: WE
authors.lastname: Neff
mesh_terms: D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006865:Hydrogenation; D008383:Margarine / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D013024:Soybean Oil / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry*
chemical_list: D014280:Triglycerides; D013024:Soybean Oil; D008383:Margarine