Determination of endocrine disruptors in environmental waters using poly(acrylamide-vinylpyridine) monolithic capillary for in-tube solid-phase microextraction coupled to high-performance liquid chromatography with fluorescence detection.
A method for the determination of endocrine disruptors, bisphenol A and 17alpha-ethinylestradiol, in environmental water samples was developed using in-tube solid-phase microextraction followed by liquid chromatography and fluorescence detection. A poly(acrylamide-vinylpyridine) monolithic capillary column was applied as the extraction media in view of its greater phase ratio than open-tubular capillaries and thus higher extraction efficiency. After optimizing the extraction conditions, bisphenol A and 17alpha-ethinylestradiol were extracted directly from water samples in a wide dynamic linear range of 0.5-1000 ng mL(-1), with the detection limits obtained as 0.064 and 0.12 ng mL(-1), respectively. The precision of the method was satisfactory with the intraday and interday RSD values smaller than 7.2%. Environmental water samples of different sources were successfully analyzed with the presented method and the monolithic capillary was proved to be robust and reusable in analyzing real water samples.
Fan
Yi
Y
Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
Zhang
Min
M
Da
Shi-Lu
SL
Feng
Yu-Qi
YQ
D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004702:Endocrine Glands / Q000187:drug effects; D008832:Microchemistry; D009641:Norethynodrel / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D009641:Norethynodrel; D010636:Phenols; D014874:Water Pollutants, Chemical; C002029:17alpha-ethynylestr-5(10)-ene-3alpha,17beta-diol; C006780:bisphenol A; D000098826:Bisphenol A Compounds