Indications of limited altered gene expression in Pinus sylvestris trees from the Chernobyl region.
To evaluate the consequences of irradiation on the vegetation of the Chernobyl region, gene expression was compared in morphologically normal and dwarf needles from the same Pinus sylvestris trees in a region where the absorbed dose was 3-5 Gy. To compare the levels of gene expression, arrays consisting of 373 Pinus taeda cDNAs were hybridized with labeled cDNA derived from normal and dwarf needles of P. sylvestris. Twelve genes were significantly (P<0.01) up- or down-regulated between normal and dwarf needles for all five trees taken together. Five of these, related to stress or development, were up- or down-regulated 1.25-1.7-fold in the dwarf needles. There were no significant differences in (137)Cs content in the normal and dwarf needles, or in elongation growth rate of seedlings raised under controlled conditions from seed derived from trees in the region that had received a radiation dose over the range 2-12 Gy.
Zelena
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Department of Biophysics and Radiobiology, Institute of Cell Biology and Genetic Engineering, 148 Zabolotnogo Street, Kyiv 03143, Ukraine.
Sorochinsky
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von Arnold
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van Zyl
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Clapham
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DH
D002588:Cesium Radioisotopes / Q000032:analysis; D047852:Chernobyl Nuclear Accident; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D049673:History, 20th Century; D020411:Oligonucleotide Array Sequence Analysis; D041605:Pinus sylvestris / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism; D011847:Radioactive Fallout; D014455:Ukraine
D002588:Cesium Radioisotopes; D004785:Environmental Pollutants; D011847:Radioactive Fallout