title: Feasibility of implementing an automated culture system for bacteria screening in platelets in the blood bank routine.
abstract: Bacterial contamination of blood components is the principal infectious complication linked to transfusion. The aim of the study was to evaluate the applicability of an automated culture system for platelets. 10 141 platelet concentrates were cultured individually and in pools of five on storage days 1 and 7 using Bact/Alert system aerobic bottles. A modified collection bag was used for improved sampling. Five-millilitre samples were cultured at 37 degrees C for 7 days. Only those samples where the same bacteria were identified in reculture were considered true positives (TP). Homogeneity of proportions was tested by Fisher's exact test. The rate of TP was 30 per 100 000 (95% CI, 6.1-86.4) sampling on day 1; 33 per 100 000 (95% CI, 7-96) on day 7; and 40 per 100 000 (95% CI, 1.28-122.4) if the screening was based on taking both samples (day 1 and 7). Only one TP was detected in the pool testing. The time for detection among TPs on day 1 ranged between 30 and 134 h. The system is not considered practical for use as a routine screening method, as the time for detection is too long. Pool testing is insensitive. Faster screening methods or pathogen-inactivation systems are needed.
authors.affiliation: Centro de Donación de Sangre Cruz Roja Española, Madrid, Spain. ecastro@cdscruzroja.infonegocio.com
authors.forename: E
authors.initials: E
authors.lastname: Castro
authors.forename: J L
authors.initials: JL
authors.lastname: Bueno
authors.forename: L
authors.initials: L
authors.lastname: Barea
authors.forename: R
authors.initials: R
authors.lastname: González
mesh_terms: D001419:Bacteria; D001424:Bacterial Infections / Q000517:prevention & control; D001431:Bacteriological Techniques / Q000379:methods*; D000094902:Blood Banking / Q000379:methods; D001792:Blood Platelets / Q000382:microbiology*; D015169:Colony Count, Microbial; D006801:Humans