Production of a transgenic pig expressing human albumin and enhanced green fluorescent protein.
We introduced a fusion gene of human albumin and enhanced green fluorescent protein (EGFP) into porcine oocytes using the sperm vector method, and produced a piglet that showed clear expression of GFP in the hooves and skin. PCR and Southern blotting analysis of genomic DNA extracted from the piglet's tissues, including the liver, showed that the tissues carried the transgene. RT-PCR analysis demonstrated that both the human albumin and EGFP genes were expressed in the tissues. The fact that human albumin gene was integrated and expressed in the liver of the transgenic pig opened a way for us to achieve our goal, which was the use of transgenic pigs for the bioartificial liver support system.
Naruse
Katsutoshi
K
Division of Artificial Organs & Transplantation, Department of Surgery, Faculty of Medicine, University of Tokyo, Japan. narusek-sur@h.u-tokyo.ac.jp
Ishikawa
Hiroshi
H
Kawano
Hiro-omi
HO
Ueda
Hideto
H
Kurome
Mayuko
M
Miyazaki
Koji
K
Endo
Maiko
M
Sawasaki
Tohru
T
Nagashima
Hiroshi
H
Makuuchi
Masatoshi
M
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D002645:Chickens; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009865:Oocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D019076:Transgenes
D000418:Albumins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D004247:DNA