title: Expression of cell survival/death genes: Bcl-2 and Bax at the rate of colon cancer prognosis.
abstract: The rate of tumour growth is dependent on the balance between proliferation and apoptosis at all stages of carcinogenesis. Apoptosis inhibition, in turn, depends partly on the balance between expression of two cell death regulatory genes, Bcl-2 and Bax. Colon cancer has long been associated with disturbances in apoptosis regulation. The aim of our study was to determine the expression levels of Bcl-2 and Bax mRNAs in 1 microg sample of total RNA obtained from normal colon and colon adenocarcinoma. This study was intended to evaluate possible differences in Bcl-2 and Bax mRNA levels at particular stages of colon adenocarcinoma classified according to Duke's system. The apoptotic frequency (represented by Bax mRNA copy number) was inversely proportional to the decrease of Bcl-2 gene expression. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was performed to confirm apoptosis.
authors.affiliation: Department of Molecular Biology and Medical Genetic, Medical University of Silesia, Katowice, Poland. mpaul@slam.katowice.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Paul-Samojedny
authors.forename: Danuta
authors.initials: D
authors.lastname: Kokocińska
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Samojedny
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Robert
authors.initials: R
authors.lastname: Partyka
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Lorenz
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016923:Cell Death; D002470:Cell Survival / Q000235:genetics; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D012313:RNA