title: Interactions of drugs and an oligonucleotide with charged membranes analyzed by immobilized liposome chromatography.
abstract: We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or distearoyltrimethylammoniumpropane) or detergent (sodium dodecylsulfate) interacted electrostatically in a concentration-dependent matter with the solutes. The oligonucleotide ions presumably bound to the liposomes by multipoint interactions, which was saturable. Sodium dodecylsulfate seemed to affect the drug-membrane interactions more strongly than phosphatidylserine did, probably due to different positioning in the bilayer.
authors.affiliation: Department of Biochemistry, Biomedical Center, Uppsala University, Box 576, SE-751 23 Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Lundquist
authors.forename: Caroline
authors.initials: C
authors.lastname: Engvall
authors.forename: Elisabet
authors.initials: E
authors.lastname: Boija
authors.forename: Sanela
authors.initials: S
authors.lastname: Kurtovic
authors.forename: Jyoti
authors.initials: J
authors.lastname: Chattopadhyaya
authors.forename: Christine Lagerquist
authors.initials: CL
authors.lastname: Hägglund
authors.forename: Per
authors.initials: P
authors.lastname: Lundahl
mesh_terms: D001483:Base Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D008081:Liposomes; D008567:Membranes, Artificial; D009841:Oligonucleotides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*
chemical_list: D008081:Liposomes; D008567:Membranes, Artificial; D009841:Oligonucleotides; D004364:Pharmaceutical Preparations