title: Ongoing pregnancy after ICSI of frozen-thawed PESA-retrieved spermatozoa and IVF in a controlled natural cycle.
abstract: The recovery of a mature oocyte from a natural cycle followed by IVF (nIVF) is an attractive alternative to conventional IVF, involving ovarian stimulation, in the treatment of female infertility. Similarly, surgical recovery of spermatozoa from the epididymis by percutaneous sperm aspiration (PESA) has simplified the treatment of men with obstructive azoospermia. A couple sought treatment for diminished ovarian reserve and male factor infertility using IVF. A mature oocyte was retrieved and was inseminated by intracytoplasmic sperm injection (ICSI), following recovery of spermatozoa by PESA. A good quality embryo was transferred. A viable pregnancy was confirmed by ultrasound scan. A healthy baby boy was delivered naturally at 37 weeks gestation. This study reports the first ongoing clinical pregnancy and subsequent birth resulting from ICSI of spermatozoa retrieved by PESA into an oocyte recovered during a natural cycle. The use of a combination of less invasive assisted reproductive techniques (PESA and nIVF) can overcome barriers to fertility.
authors.affiliation: Department of Obstetrics and Gynecology, Université de Montréal and Saint Luc Hospital (CHUM), 1058 rue Saint-Denis, Montréal, Québec, Canada H2X 3J4. kadoch@sympatico.ca
authors.forename: Isaac Jacques
authors.initials: IJ
authors.lastname: Kadoch
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Phillips
authors.forename: Robert
authors.initials: R
authors.lastname: Hemmings
authors.forename: Louise
authors.initials: L
authors.lastname: Lapensée
authors.forename: Bernard
authors.initials: B
authors.lastname: Couturier
authors.forename: François
authors.initials: F
authors.lastname: Bissonnette
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D010062:Ovulation Induction / Q000379:methods; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D020858:Tissue and Organ Harvesting / Q000379:methods*