[Utilization of released proteins (RPs) of Yersinia enterocolitica for serologic diagnosis of yersiniosis. III. Western-blot].
The usefulness of the immunoblotting using released proteins (Yersinia outer proteins-Yop) as the antigen for the serological diagnosis of yersiniosis was estimated. The IgA, IgG, and IgM antibody responses of patients with yersiniosis and healthy blood donors were studied by western-blot prepared in our laboratory, and two commercial assays. The results indicate that antibodies of all three classes are most consistently directed against the proteins of YopD, YopM and YopE. Good correlations between the three western-blots were obtained for all proteins except the protein V-AG. Patients with yersinia-triggered reactive arthritis have IgA class antibodies against the YopD more often and for longer period than the non-arthritic patients with yersiniosis.
Rastawicki
Waldemar
W
Zakład Bakteriologii PZH w Warszawie.
Jagielski
Marek
M
Gierczyński
Rafał
R
D000907:Antibodies, Bacterial / Q000097:blood*; D001170:Arthritis, Infectious / Q000276:immunology / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D015009:Yersinia Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C078910:YopD protein, Yersinia; C063239:yopE protein, Yersinia; C060772:yopM protein, Yersinia