title: Comparison of early passage, senescent and hTERT immortalized endothelial cells.
abstract: The need for standardized experimental conditions to gain relevant and reproducible results has increased the demand for well characterized continuously growing cell lines that exhibit the characteristics of their normal counterparts. Immortalization of normal human cells by ectopic expression of the catalytic subunit of human telomerase (hTERT) has shown to result in highly differentiated cell lines. However, the influence of the increased telomerase activity on the protein expression profile was not investigated so far. Therefore, we have immortalized human umbilical vein endothelial cells (HUVECs) by hTERT overexpression and compared them to their normal early passage and senescent counterparts. This study, including a proteomic approach, shows that ectopic hTERT expression leads to a stable growing cell line. Although these cells are highly differentiated, the protein expression profile of the cell line is different to that of normal early passage and senescent cells.
authors.affiliation: Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Martina Wei-Fen
authors.initials: MW
authors.lastname: Chang
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Corina
authors.initials: C
authors.lastname: Mayrhofer
authors.forename: Klaus
authors.initials: K
authors.lastname: Fortschegger
authors.forename: Günter
authors.initials: G
authors.lastname: Allmaier
authors.forename: Gorji
authors.initials: G
authors.lastname: Marzban
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Regina
authors.initials: R
authors.lastname: Voglauer
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000166:cytology* / Q000473:pathology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D016133:Polymerase Chain Reaction; D040901:Proteomics; D019098:Telomerase / Q000235:genetics; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D019098:Telomerase