title: Exposure to chemokines during maturation modulates antigen presenting cell function of mature macrophages.
abstract: Macrophages generated with macrophage-colony stimulating factor (M-CSF) are defective in antigen presenting cell (APC) function, although they do express major histocompatibility (MHC) class II molecules, numerous accessory molecules, and intercellular adhesion molecules. In the present study, we show evidence that the acquisition of APC function is influenced significantly by microenvironmental condition of development. Macrophages generated from bone marrow progenitor cells with M-CSF and interleukin (IL)-6 were defective in APC function as determined by their ability to induce anti-CD3 monoclonal antibody (mAb)-primed T cell proliferation. Macrophages generated in the presence of some of the CC chemokines such as leukotactin-1, macrophage inflammatory protein (MIP)-1alpha, and RANTES together with M-CSF and IL-6, however, induced proliferation of anti-CD3 mAb-primed T cells. Maximum level of APC function was obtained when developing macrophages were exposed with the chemokines at the late stage of maturation. Enhanced APC function of the macrophages appeared to be correlated with the expression of co-stimulatory molecules and the ability to produce cytokines. These results suggest that the acquisition of APC function of mature macrophage is modulated significantly by the microenvironmental condition during development.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jae Kwon
authors.initials: JK
authors.lastname: Lee
authors.forename: Jeong Ki
authors.initials: JK
authors.lastname: Kim
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Lee
authors.forename: Hyeon Seon
authors.initials: HS
authors.lastname: Kim
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Im
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Kim
authors.forename: Chong-Kil
authors.initials: CK
authors.lastname: Lee
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018925:Chemokines / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype
chemical_list: D018925:Chemokines; D016173:Macrophage Colony-Stimulating Factor