title: Temporal progression of viral replication and gross and histological lesions in Balb/c mice inoculated epidermally with Saimiriine herpesvirus 1 (SaHV-1).
abstract: Saimiriine herpesvirus 1 (SaHV-1), an alphaherpesvirus enzootic in squirrel monkeys, is genetically related to monkey B virus and human herpes simplex virus (HSV). To study the temporal progression of viral spread and associated lesions, Balb/c mice were inoculated epidermally by scarification with a green fluorescent protein (GFP)-expressing recombinant strain of SaHV-1 and killed sequentially. Pinpoint ulcerative lesions in the inoculated epidermis progressed over a few days to unilateral or bilateral hindlimb paresis or paralysis, urinary and faecal incontinence, abdominal distension, hunched posture and eventual depression warranting euthanasia. Viral replication was present within epidermal keratinocytes, neurons of the dorsal root ganglia and thoracolumbar spinal cord, regional autonomic ganglia, lower urinary tract epithelium and colonic myenteric plexuses, as indicated by histological lesions and GFP expression. Almost all mice inoculated with 10(5) or 10(6) plaque-forming units (PFU) of SaHV-1 developed rapidly progressive disease. Two of eight mice given 10(4)PFU developed disease, but no mice receiving less than 10(4)PFU gave evidence of infection. Mice that showed no clinical signs also failed to develop an antiviral IgG response, indicating absence of active viral infection. For SaHV-1 inoculated epidermally, the ID(50), CNSD(50) and LD(50) values were identical (10(4.38)), indicating that successful infection by this route invariably resulted in lethal CNS (central nervous system) disease. Consistently severe disease in all infected animals, with regionally extensive distribution of viral replication, constituted a marked difference from the disease produced by intramuscular inoculation.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Breshears
authors.forename: R
authors.initials: R
authors.lastname: Eberle
authors.forename: J W
authors.initials: JW
authors.lastname: Ritchey
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000473:pathology* / Q000821:virology; D005260:Female; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006561:Herpes Simplex / Q000473:pathology* / Q000635:transmission* / Q000821:virology; D015603:Keratinocytes / Q000473:pathology / Q000821:virology; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000472:pathogenicity / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000956:Antigens, Viral; D049452:Green Fluorescent Proteins