Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates.
Phylogenetic relationships within the genus Pseudomonas were examined by comparing partial (about 1000 nucleotides) rpoB gene sequences. A total of 186 strains belonging to 75 species of Pseudomonas sensu stricto and related species were studied. The phylogenetic resolution of the rpoB tree was approximately three times higher than that of the rrs tree. Ribogroups published earlier correlated well with rpoB sequence clusters. The rpoB sequence database generated by this study was used for identification. A total of 89 isolates (79.5%) were identified to a named species, while 16 isolates (14.3%) corresponded to unnamed species, and 7 isolates (6.2%) had uncertain affiliation. rpoB sequencing is now being used for routine identification of Pseudomonas isolates in our laboratory.
Ait Tayeb
Lyneda
L
Unité de Biodiversité des Bactéries Pathogènes Emergentes, INSERM Unit 389, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Ageron
Elisabeth
E
Grimont
Francine
F
Grimont
P A D
PA
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit