QCM-D on mica for parallel QCM-D-AFM studies.
Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coated QCM-D sensors limits parallel structural characterization by atomic force microscopy (AFM). We present a method for coating QCM-D sensors with thin mica sheets operating in liquid with high stability and sensitivity. We define criteria to objectively assess the reliability of the QCM-D measurements and demonstrate that the mica-coated sensors can be used to follow the formation of supported lipid membranes and subsequent protein adsorption. This method allows combining QCM-D and AFM investigations on identical supports, providing detailed physicochemical and structural characterization of model membranes.
Richter
Ralf P
RP
Laboratoire d'Imagerie Moléculaire et Nano-Bio-Technologie, IECB, UMR-CNRS 5471, Université Bordeaux I, 2 Rue Robert Escarpit, 33607 Pessac Cedex, France.
Brisson
Alain
A
D000538:Aluminum Silicates / Q000737:chemistry*; D017304:Annexin A5 / Q000737:chemistry; D003460:Crystallization; D008051:Lipid Bilayers / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D011791:Quartz / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water
D000538:Aluminum Silicates; D017304:Annexin A5; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D014867:Water; D011791:Quartz; C042511:1,2-dioleoylphosphatidylserine; C017251:1,2-oleoylphosphatidylcholine; C011934:mica