Isolation and expression analysis of genes encoding DNA methyltransferase in wheat (Triticum aestivum L.).
DNA methylation of cytosine residues, catalyzed by DNA methyltransferases, is suggested to play important roles in regulating gene expression and plant development. In this study, we isolated four wheat cDNA fragments and one cDNA with open reading frame encoding putative DNA methyltransferase and designated TaMET1, TaMET2a, TaMET2b, TaCMT, TaMET3, respectively. BLASTX searches and phylogenetic analysis suggested that five cDNAs belonged to four classes (Dnmt1, Dnmt2, CMT and Dnmt3) of DNA methyltransferase genes. TaMET2a encoded a protein of 376 aa and contained eight of ten conserved motifs characteristic of DNA methyltransferase. Genomic sequence of TaMET2a was obtained and found to contain ten introns and eleven exons. The expression analysis of the five genes revealed that they were expressed in developing seed, during germination and various vegetative tissues, but in quite different abundance. It was interesting to note that TaMET1 and TaMET3 mRNAs were clearly detected in dry seeds. Moreover, the differential expression patterns of five genes were observed between wheat hybrid and its parents in leaf, stem and root of jointing stage, some were up-regulated while some others were down-regulated in the hybrid. We concluded that multiple wheat DNA methyltransferase genes were present and might play important roles in wheat growth and development.
Dai
Yan
Y
Department of Plant Genetics and Breeding, State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing.
Ni
Zhongfu
Z
Dai
Jing
J
Zhao
Tao
T
Sun
Qixin
Q
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D006824:Hybridization, Genetic; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D016415:Sequence Alignment; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D004248:DNA (Cytosine-5-)-Methyltransferases