title: Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags.
abstract: Phytophthora parasitica is a soilborne oomycete pathogen capable of infecting a wide range of plants, including many solanaceous plants. In a first step towards large-scale gene discovery, we generated expressed sequence tags (ESTs) from a cDNA library constructed using mycelium grown in synthetic medium. A total of 3568 ESTs were assembled into 2269 contiguous sequences. Functional categorization could be performed for 65.45% of ESTs. A significant portion of the transcripts encodes proteins of common metabolic pathways. The most prominent sequences correspond to members of the elicitin family, and enzymes involved in the lipid metabolism. A number of genes potentially involved in pathogenesis were also identified, which may constitute virulence determinants.
authors.affiliation: INRA UMR 1064, Unité Interactions Plantes-Microorganismes et Santé Végétale, 400 route des Chappes, F-06930 Sophia-Antipolis cedex, France. panab@antibes.inra.fr
authors.forename: Franck
authors.initials: F
authors.lastname: Panabières
authors.forename: Joëlle
authors.initials: J
authors.lastname: Amselem
authors.forename: Eric
authors.initials: E
authors.lastname: Galiana
authors.forename: Jo-Yanne
authors.initials: JY
authors.lastname: Le Berre
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D010802:Phylogeny; D010838:Phytophthora / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D020543:Proteome; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D020543:Proteome