Interactions of acinar cells on biomaterials with various surface properties.
The purpose of this study is to evaluate the interactions of rat parotid acinar cells on biomaterials with different surface properties. The biomaterials used in this study included polyvinyl alcohol (PVA), chitosan, poly (ethylene-co-vinyl alcohol) (EVAL), and polyvinylidene fluoride (PVDF). Cell morphology was observed by photomicroscope. Cell growth and differentiated characteristic function were separately assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity and amylase activity. Results indicated that behaviors of acinar cells on materials might differ to a great extent depending on the surface hydrophilicity and morphology of the materials. On the relatively hydrophobic materials, the abilities of acinar cells to adhere and proliferate increased simultaneously. In addition, porous PVDF had higher cell growth compared with dense PVDF. Therefore, the hydrophobic PVDF with a porous structure was the best substrate for culturing acinar cells. According to our findings, a tubular PVDF scaffold with dense outer surface to prevent saliva leakage and with porous inner surface for the cell growth was proposed to serve as an artificial salivary gland for future use in the treatment of patients with salivary hypofunction.
Chen
Min-Huey
MH
National Taiwan University Hospital, No 1 Chang-Te Street, Tapei 100, Taiwan, Republic of China.
Hsu
Yuan-Hua
YH
Lin
Chun-Pin
CP
Chen
Yi-Jane
YJ
Young
Tai-Horng
TH
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002455:Cell Division; D002478:Cells, Cultured; D007797:Laminin / Q000502:physiology; D010179:Pancreas / Q000166:cytology* / Q000201:enzymology; D011145:Polyvinyls; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D005465:Fluorocarbon Polymers
D000681:Amylases; D001672:Biocompatible Materials; D007797:Laminin; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers