title: Suppressive effect of rice extract on Helicobacter pylori infection in a Mongolian gerbil model.
abstract: BACKGROUND
  Rice extract has been shown to protect gastric mucosa from stress-induced damage. In this study, the antibiotic effect and the anti-inflammatory effect of orally administered aqueous rice extract on Helicobacter pylori infection and H. pylori-induced gastritis, respectively, in Mongolian gerbils were investigated.
  
  
  METHODS
  Fifty specific-pathogen-free male Mongolian gerbils, seven weeks old, were divided into four groups: uninfected, untreated animals (group A); uninfected, rice extract-treated animals (group B); H. pylori-infected, untreated animals (group C); and H. pylori-infected, rice extract-treated animals (group D). Group C and D animals were killed 12 weeks after H. pylori infection (i.e., at 19 weeks of age) and group A and B animals were also killed at age 19 weeks. The stomachs were removed for histopathological examination with hematoxylin-and-eosin staining and anti-5'-bromo-2'-deoxyuridine (BrdU) immunostaining, and to determine the bacterial burden. Serum anti-H. pylori antibody titers were also tested.
  
  
  RESULTS
  In groups A and B, the gastric mucosa showed no inflammatory cell infiltration and a few BrdU-reactive cells. Group C animals developed marked chronic active gastritis in the gastric mucosa, and BrdU-labeled cells in the gastric mucosa markedly increased in number. In group D animals, a significant reduction occurred in the degree of neutrophilic polymorphonuclear cell infiltration into the gastric mucosa, in the BrdU-labeling indices of gastric epithelial cells, and in anti-H. pylori antibody titers in the serum (P < 0.01), compared with although H. pylori was not completely eradicated.
  
  
  CONCLUSIONS
  The rice extract was effective in suppressing inflammation and epithelial cell proliferation in the gastric mucosa in H. pylori-infected Mongolian gerbils. The rice extract has potential to exhibit a protective effect on H. pylori-related gastric mucosal diseases.
authors.affiliation: Department of Gastroenterological Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Murakami
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishizone
authors.forename: Fukuto
authors.initials: F
authors.lastname: Maruta
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Akita
authors.forename: Yukie
authors.initials: Y
authors.lastname: Okimura
authors.forename: Toshiko
authors.initials: T
authors.lastname: Kumagai
authors.forename: Megumi
authors.initials: M
authors.lastname: Jo
authors.forename: Takashi
authors.initials: T
authors.lastname: Tokuyama
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D005756:Gastritis / Q000188:drug therapy* / Q000382:microbiology; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000188:drug therapy*; D007150:Immunohistochemistry; D008297:Male; D012275:Oryza; D008517:Phytotherapy / Q000379:methods*; D028321:Plant Preparations / Q000627:therapeutic use*; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D016896:Treatment Outcome
chemical_list: D028321:Plant Preparations