title: Specific properties of heavy fraction of mitochondria from human-term placenta - glycerophosphate-dependent hydrogen peroxide production.
abstract: Mitochondrial respiratory chain enzyme Complexes are present in placenta at proportion similar to other tissues with exception of glycerophosphate dehydrogenase (mGPDH) which is expressed at a very high rate. As shown by Western blot quantification and respiratory chain enzyme activity measurements, the specific content of mGPDH is similar to that of succinate dehydrogenase or NADH dehydrogenase. Using fluorometric probe dichlorodihydrofluorescein diacetate we found that placental mitochondria display high rate of glycerophosphate-dependent hydrogen peroxide production. This was confirmed by oxygraphic detection of glycerophosphate-induced, KCN- or antimycin A-insensitive oxygen uptake. Hydrogen peroxide production by mGPDH was highly activated by one-electron acceptor, potassium ferricyanide and it was depressed by inhibitors of mGPDH and by cytochrome c. Our results indicate that mGPDH should be considered as an additional source of reactive oxygen species participating in induction of oxidative stress in placenta.
authors.affiliation: Department of Pediatrics, 1st Faculty of Medicine, Charles University, Ke Karlovu 2, 12000 Prague 2, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Honzík
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
authors.forename: M
authors.initials: M
authors.lastname: Böhm
authors.forename: P
authors.initials: P
authors.lastname: Jesina
authors.forename: T
authors.initials: T
authors.lastname: Mrácek
authors.forename: J
authors.initials: J
authors.lastname: Paul
authors.forename: J
authors.initials: J
authors.lastname: Zeman
authors.forename: J
authors.initials: J
authors.lastname: Houstek
mesh_terms: D000818:Animals; D006224:Cricetinae; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D010100:Oxygen