title: Toxicity to early life stages and an estrogenic effect of a bisphenol A metabolite, 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene on the medaka (Oryzias latipes).
abstract: In a recent study, it was reported that 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP), a metabolite of bisphenol A (BPA; 2,2-bis(4-hydroxyphenyl)propane), showed estrogenic activity in several in vitro assays, and the estrogenic activity of MBP was higher than that of BPA. In this study, we have investigated the early life stage toxicity and estrogenic effect of MBP on medaka (Oryzias latipes). The 96-h median lethal concentration value of MBP and BPA with 24-h-old larvae was estimated to be 1,640 and 13,900 microg/l, respectively. The hatchability of fertilized eggs exposed to MBP and BPA over 14 days was significantly decreased at doses of 2,500 microg/l and 12,500 microg/l, respectively. Moreover, to compare the potency of estrogenic activity in vivo, male medaka were exposed to various concentrations of MBP and BPA for 21 days. The lowest-observed-effect concentrations of MBP and BPA for hepatic vitellogenin induction in male medaka were estimated to be 4.1 and 1,000 microg/l, respectively. These results suggest that MBP has high toxicity for early life stages of the medaka, and that the estrogenic activity of MBP was about 250-fold higher than that of BPA to male medaka.
authors.affiliation: Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan. hiroishi@agr.ehime-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Watanabe
authors.forename: Naomi
authors.initials: N
authors.lastname: Matsumura
authors.forename: Masashi
authors.initials: M
authors.lastname: Hirano
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nagao
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiratsuchi
authors.forename: Shinya
authors.initials: S
authors.lastname: Kohra
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yoshihara
authors.forename: Koji
authors.initials: K
authors.lastname: Arizono
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D001835:Body Weight / Q000187:drug effects; D004625:Embryo, Nonmammalian; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D006066:Gonads / Q000187:drug effects / Q000254:growth & development; D006128:Growth / Q000187:drug effects; D007814:Larva; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009990:Oryzias / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology* / Q000633:toxicity; D015996:Survival Rate; D014819:Vitellogenins / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D014819:Vitellogenins; C504750:4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene; C006780:bisphenol A; D000098826:Bisphenol A Compounds