title: [Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection].
abstract: OBJECTIVE
  To assess the value of dynamic monitoring of soluble human lymphocytic antigen-I (sHLA-I) in the prediction of graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (PBSCT).
  
  
  METHODS
  Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantitatively detect serum sHLA-I. The serum samples for testing were added into W6 32 monoclonal antibody-coated microtiter plate and incubated with anti-beta2m HRP followed by color development with the addition of the substrate. Serum sHLA-I level was measured in 63 healthy blood donors of Shanghai and in 24 PBSCT recipients before and and at different time points after the operation.
  
  
  RESULTS
  No changes in sHLA-I levels occurred in allogeneic PBSCT recipients without GVHD or with only grade I GVHD, but sHLA-I reached high levels in patients suffering GVHD II-IV(P<0.05), which was effectively lowered by the application of immunosuppressants.
  
  
  CONCLUSIONS
  Measurements of sHLA-I levels can be valuable for monitoring GVHD after PBSCT.
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xia
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Qiu
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Cao
authors.forename: Jiong-cai
authors.initials: JC
authors.lastname: Lan
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Wen
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000175:diagnosis*; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*
chemical_list: D015395:Histocompatibility Antigens Class I