title: Oligodendrocyte-myelin glycoprotein is present in lipid rafts and caveolin-1-enriched membranes.
abstract: The oligodendrocyte-myelin glycoprotein is a ligand of the neuronal Nogo receptor and a potent inhibitor of neurite outgrowth, but its physiological function remains to be elucidated. The oligodendrocyte-myelin glycoprotein is anchored solely in the outer leaflet of the plasma membrane via its glycosylphosphatidylinositol anchor, and through its leucine-rich repeat domain, it likely interacts with other proteins. In the present study, we compare its buoyancy and detergent solubility characteristics with those of other myelin proteins. Based on its detergent solubility profile and membrane fractionation using established ultracentrifugation procedures, we conclude that the oligodendrocyte-myelin glycoprotein is a lipid raft component that is closely associated with the axolemma. Moreover, it associates with caveolin-1 and caveolin-1-enriched membranes. We postulate that, by virtue of its concentration in lipid rafts and perhaps through interactions with caveolin-1, the oligodendrocyte-myelin glycoprotein may influence signaling pathways.
authors.affiliation: Department of Neurology, University of Michigan, Ann Arbor, MI 48109-0322, USA.
authors.forename: Madanamohan
authors.initials: M
authors.lastname: Boyanapalli
authors.forename: Vicky
authors.initials: V
authors.lastname: Kottis
authors.forename: Oscar
authors.initials: O
authors.lastname: Lahoud
authors.forename: Saoussen
authors.initials: S
authors.lastname: Bamri-Ezzine
authors.forename: Peter E
authors.initials: PE
authors.lastname: Braun
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Mikol
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D058851:GPI-Linked Proteins; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000737:chemistry / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein / Q000737:chemistry / Q000378:metabolism*; D063308:Myelin-Oligodendrocyte Glycoprotein; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D012995:Solubility
chemical_list: D051242:Caveolin 1; D003902:Detergents; D058851:GPI-Linked Proteins; C571327:Mog protein, mouse; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; C560529:Omg protein, mouse