Effect of dietary fat on skin reactivity against histamine, Th1 and Th2 cytokine levels and some serum parameters in mice.
Changes in diet may be associated with the increase in allergic disease; change to high-calorie and high-fat diets may be a factor. In this study our objective was to determine skin reactivity of histamine and serum cytokine concentrations in mice fed diets containing different amounts of fat. Histamine reactivity was performed on mice back skin and serum cytokine concentrations were measured by ELISA in mice injected with anti-CD3 antibody. We measured serum interferon-gamma as a Th1-type cytokine and interleukin-4 as a Th2-type cytokine. Mice fed a high fat diet displayed enhanced skin reactivity of histamine and higher IL-4 levels in serum. These data suggest that a high fat diet may play a role in enhancing allergic reactions.
Yamaki
Kohji
K
National Agricultural Research Center for Tohoku Region, National Agricultural Research Organization, 50 Harajuku-Minami, Arai Fukushima 960-2156, Japan. kyamaki@affrc.go.jp
Takano-Ishikawa
Yuko
Y
Goto
Masao
M
Shinohara
Kazuki
K
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D017252:CD3 Complex / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects; D016207:Cytokines / Q000097:blood*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism*; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012867:Skin / Q000098:blood supply / Q000187:drug effects*; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*
D000906:Antibodies; D017252:CD3 Complex; D016207:Cytokines; D004041:Dietary Fats; D007136:Immunoglobulins; D006632:Histamine