title: Ets-1 expression promotes epithelial cell transformation by inducing migration, invasion and anchorage-independent growth.
abstract: Ets-1 is the prototype of the family of ETS transcription factors. In human tumors, Ets-1 is expressed in endothelial cells and fibroblasts of the tumor stroma and is proposed to play a role in tumor vascularization and invasion by upregulating expression of matrix-degrading proteases. In human carcinomas, Ets-1 is also expressed by neoplastic cells, but little is known about the functional implications of this observation. We have addressed the role of Ets-1 in epithelial HeLa tumor cells by selecting stably Ets-1 over and underexpressing HeLa cells. Ets-1 expression increases the transformed phenotype of HeLa cells, by promoting cell migration, invasion and anchorage-independent growth, while Ets-1 downregulation reduces cell attachment. In correlation with these results, Ets-1 upregulation increases integrinbeta2 expression but not that of other integrins. These results suggest that, in addition to its role in the tumor stroma, Ets-1 may also promote tumor development and progression by increasing neoplastic transformation.
authors.affiliation: Institute of Pathology, University of Bonn, PO Box 2120, 53011 Bonn, Germany.
authors.forename: Jens Claus
authors.initials: JC
authors.lastname: Hahne
authors.forename: Ali Fuat
authors.initials: AF
authors.lastname: Okuducu
authors.forename: Annette
authors.initials: A
authors.lastname: Kaminski
authors.forename: Alexandra
authors.initials: A
authors.lastname: Florin
authors.forename: Fabrice
authors.initials: F
authors.lastname: Soncin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wernert
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018450:Disease Progression; D004847:Epithelial Cells / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D010641:Phenotype; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000502:physiology*; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000096:biosynthesis* / Q000502:physiology*
chemical_list: C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors