Screening of the antigen epitopes of basic fibroblast growth factor by phage display.
In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.
Junjian
Xiang
X
Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University, Guangzhou, 510632, China. txjj@jnu.edu.cn
Zhenyu
Zhong
Z
Ning
Deng
D
Zhendong
Zhong
Z
Hongyu
Yang
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D016222:Fibroblast Growth Factor 2 / Q000276:immunology*; D006801:Humans; D051379:Mice; D019151:Peptide Library
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019151:Peptide Library; D016222:Fibroblast Growth Factor 2; D004247:DNA