Cross-linked leucaena seed gum matrix: an affinity chromatography tool for galactose-specific lectins.
A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactose-specific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US dollars/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins.
Seshagirirao
Kottapalli
K
Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India. kssl@uohyd.ernet.in
Leelavathi
Chaganti
C
Sasidhar
Vemula
V
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000032:analysis*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification*; D012639:Seeds; D029778:Trichosanthes / Q000737:chemistry*
D037102:Lectins; D005690:Galactose