title: Determination of oxaliplatin in human plasma and plasma ultrafiltrate by graphite-furnace atomic-absorption spectrometry.
abstract: A method for sensitive determination of the anti-cancer agent oxaliplatin in human plasma and human plasma ultrafiltrate (pUF) is presented. The method is based on the quantification of platinum by graphite-furnace atomic-absorption spectrometry, with Zeeman correction and an atomisation temperature of 2,700 degrees C. Sample pretreatment involves dilution of the samples with a solution containing 0.15 mol L(-1) NaCl and 0.20 mol L(-1) HCl in water. Validation was performed in accordance with the most recent FDA guidelines for bioanalytical method validation. All results were within requirements. The validated ranges of quantification were 0.10-400 micromol L(-1) for human pUF and 0.50-400 micromol L(-1) for plasma. The assay is now successfully used to support pharmacokinetic studies of cancer patients treated with oxaliplatin.
authors.affiliation: Department of Pharmacy and Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC, Amsterdam, The Netherlands. apebr@slz.nl
authors.forename: E E M
authors.initials: EE
authors.lastname: Brouwers
authors.forename: M M
authors.initials: MM
authors.lastname: Tibben
authors.forename: M
authors.initials: M
authors.lastname: Joerger
authors.forename: O
authors.initials: O
authors.lastname: van Tellingen
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: J H M
authors.initials: JH
authors.lastname: Schellens
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000097:blood*; D000077150:Oxaliplatin; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014462:Ultrafiltration
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D006108:Graphite