NSP1 of the GRAS protein family is essential for rhizobial Nod factor-induced transcription.
Rhizobial Nod factors induce in their legume hosts the expression of many genes and set in motion developmental processes leading to root nodule formation. Here we report the identification of the Medicago GRAS-type protein Nodulation signaling pathway 1 (NSP1), which is essential for all known Nod factor-induced changes in gene expression. NSP1 is constitutively expressed, and so it acts as a primary transcriptional regulator mediating all known Nod factor-induced transcriptional responses, and therefore, we named it a Nod factor response factor.
Smit
Patrick
P
Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Wageningen 6703 HA, Netherlands.
Raedts
John
J
Portyanko
Vladimir
V
Debellé
Frédéric
F
Gough
Clare
C
Bisseling
Ton
T
Geurts
René
R
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008070:Lipopolysaccharides / Q000378:metabolism*; D029902:Medicago / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D016962:Sinorhizobium meliloti / Q000502:physiology*; D013559:Symbiosis; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D008070:Lipopolysaccharides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D017871:Calcium-Calmodulin-Dependent Protein Kinases