Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus.
Inonotus obliquus BELYU1102 was selected from 12 different strains of Inonotus as a producer of immuno-stimulating polysaccharide. After a batch fermentation of I. obliquus BELYU1102 was carried out in a 300 l pilot vessel, endo-polysaccharide and exo-polysaccharide were both obtained. The proliferation activity of endo-polysaccharide for splenic cells was much higher than the activity of exo-polysaccharide. The active endo-polysaccharide was produced primarily during the late stationary phase. Enhanced proliferation and polyclonal IgM antibody production were observed in B cells by purified water-soluble endo-polysaccharide. Nitrite production and expression of IL-1beta, IL-6, TNF-alpha, and iNOS in macrophages were also enhanced. However, the endo-polysaccharide did not affect the proliferation of T cells, the IL-2 expression of Th1 cells, or the IL-4 expression of Th2 cells. The endo-polysaccharide showed activities similar to lipopolysaccharide (LPS) for B cells and macrophages, but there was a large difference between the two polysaccharides because cellular activations induced by endo-polysaccharide were not affected by polymyxin B, a specific inhibitor of LPS. The endo-polysaccharide appeared to have other cellular binding sites with TLR-4 and did not show a direct toxicity against tumor cells. However, indirect anti-cancer effects via immuno-stimulation were observed. The mycelial endo-polysaccharide of I. obliquus is a candidate for use as an immune response modifier. Submerged mycelial cultures are advantageous for industrial production of polysaccharides.
Kim
Yong Ook
YO
Department of Biotechnology, College of Engineering, Yonsei University, Shinchon-dong, Seodaemoon-gu, Seoul 120-749, South Korea.
Han
Sang Bae
SB
Lee
Hong Woen
HW
Ahn
Hyo Jung
HJ
Yoon
Yeo Dae
YD
Jung
Joon Ki
JK
Kim
Hwan Mook
HM
Shin
Chul Soo
CS
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005285:Fermentation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009573:Nitrites / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility; D013601:T-Lymphocytes / Q000378:metabolism
D000276:Adjuvants, Immunologic; D003470:Culture Media; D016207:Cytokines; D009573:Nitrites; D011134:Polysaccharides