title: Intracellular and extracellular CPPD crystals are a regular feature in synovial fluid from uninflamed joints of patients with CPPD related arthropathy.
abstract: OBJECTIVE
  To determine whether calcium pyrophosphate dihydrate (CPPD) crystals can be found in the synovial fluid of non-inflamed joints in patients with CPPD related arthropathy; if so, to determine whether they interact with cells and produce subclinical inflammation in this setting.
  
  
  METHODS
  74 synovial fluid samples were obtained from non-inflamed knees of 74 patients with CPPD related arthropathy. Identification of CPPD crystals and synovial fluid cell counts were done manually in undiluted samples using a haematocytometric chamber. A supravital stain (Testsimplets, Boehringer Mannheim) was used to carry out differential counts and to assess the presence of intracellular crystals.
  
  
  RESULTS
  All 74 samples contained CPPD crystals. The mean cell count was 301.4 cells/microl (95% confidence interval (CI), 216.6 to 386.4; range 22 to 2302.5). Mononuclear cells accounted for 83.2% (95% CI, 80.4% to 86.1%; range 43% to 99%), the rest being polymorphonuclear (PMN) cells (16.8% (95% CI, 13.9% to 19.6%; range 1% to 57%)). All the samples contained intracellular CPPD crystals, which were found in 24.0% of all the cells (95% CI, 20.1% to 27.9%; range 1% to 78%). Most of the intracellular crystals were inside mononuclear cells (22.2% of all the cells (95% CI, CI 18.5% to 25.9%)), although some PMN also contained them (1.8% of all the cells (95% CI, 1.1% to 2.4%)).
  
  
  CONCLUSIONS
  CPPD crystals are normally found in synovial fluid of non-inflamed joints of patients with CPPD related arthropathy, and they interact with cells. The raised cell counts and percentage of PMN suggest mild subclinical inflammation in these joints.
authors.affiliation: Calle Pintor Baeza, s/n Alicante 03010, Spain. martinez_agu@gva.es
authors.forename: A
authors.initials: A
authors.lastname: Martínez Sanchis
authors.forename: E
authors.initials: E
authors.lastname: Pascual
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002131:Calcium Pyrophosphate / Q000032:analysis*; D002452:Cell Count; D002805:Chondrocalcinosis / Q000378:metabolism* / Q000473:pathology; D003460:Crystallization; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000737:chemistry* / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D013582:Synovial Fluid / Q000737:chemistry* / Q000166:cytology
chemical_list: D002131:Calcium Pyrophosphate