Luminal nitric oxide in ileal reservoirs for continent cutaneous diversion or orthotopic bladder reconstruction.
OBJECTIVE
To measure mucosal inflammation as reflected in nitric oxide (NO) production in ileal reservoirs for the storage of urine and to correlate it with the growth of bacteria as well as CRP.


METHODS
Intraluminal gas NO concentrations were determined using the chemoluminescence technique in 25 patients with continent cutaneous ileal reservoirs (Kock pouch) and 12 patients with orthotopic bladders (hemi-Kock or T-pouch). NO concentrations were determined in both intestinal reservoir gas and silicon catheter balloon gas. Urinary culture and blood CRP determinations were performed.


RESULTS
NO concentrations in reservoir gas were higher than in silicon catheter balloons. Bacteriuria was associated with approximately 20 times higher NO concentrations than sterile urine. NO concentrations did not differ between continent cutaneous reservoirs or orthotopic bladders when due attention was paid to variance in the rate of bacteriuria. Elevated CRP was associated with higher NO concentrations. Bacteriuria with acinetobacter, enterococci and pseudomonas appeared to cause comparatively lower NO concentrations. The inflammatory response of reservoir walls to bacteriuria did not decrease with time.


CONCLUSIONS
Urine in itself causes much less intestinal wall inflammation than bacteriuria, as reflected in NO production. High CRP values are associated with high NO concentrations. The inflammatory response varies with the bacterial specimens.
Pazooki
David
D
Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
Kilander
Anders
A
Lindholm
Elisabet
E
Olin
Anna-Carin
AC
Törnqvist
Harriet
H
Ung
Kjell-Arne
KA
Jonsson
Olof
O
D000388:Air / Q000032:analysis*; D001419:Bacteria / Q000302:isolation & purification; D001437:Bacteriuria / Q000097:blood / Q000382:microbiology; D002097:C-Reactive Protein / Q000378:metabolism; D039021:Colonic Pouches / Q000502:physiology*; D015169:Colony Count, Microbial; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D001745:Urinary Bladder Diseases / Q000601:surgery; D016476:Urinary Reservoirs, Continent
D009569:Nitric Oxide; D002097:C-Reactive Protein