Cutting edge: egress of newly generated plasma cells from peripheral lymph nodes depends on beta 2 integrin.
During humoral immune responses, naive B cells differentiate into Ab-secreting plasma cells within secondary lymphoid organs. Differentiating plasma cells egress from their sites of generation and redistribute to other tissues, predominantly the bone marrow and mucosal tissues. In this study, we demonstrate that within peripheral lymph nodes newly generated plasma cells localize to medullary cords which express the beta(2) integrin ligand ICAM-1. In beta(2) integrin-deficient mice plasma cells accumulate inside the lymph nodes, resulting in severely reduced plasma cell numbers in the bone marrow. Since plasma cells isolated from beta(2) integrin-deficient animals migrate efficiently into the bone marrow when transferred i.v., our findings provide profound evidence that beta(2) integrins are required for the egress of plasma cells from peripheral lymph nodes.
Pabst
Oliver
O
Institute of Immunology, Hannover Medical School, Hannover, Germany.
Peters
Thorsten
T
Czeloth
Niklas
N
Bernhardt
Günter
G
Scharffetter-Kochanek
Karin
K
Förster
Reinhold
R
D019264:Adoptive Transfer; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018821:CD18 Antigens / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D018655:Lymphocyte Count; D008643:Mesentery; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009333:Neck; D009596:Nitrophenols / Q000008:administration & dosage / Q000276:immunology; D010648:Phenylacetates; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
D018821:CD18 Antigens; D006241:Haptens; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D018799:Intercellular Adhesion Molecule-1; D002772:Cholera Toxin