[Study of the genetic stability of expression plasmid vector pMG36e in host bacteria].
OBJECTIVE
To study the genetic stability of expression plasmid vector pMG36e in Escherichia coli JM109 and Lactococcus lactis MG1363/36e and observe the effect of erythomycin for its stability.


METHODS
We used classical method to determine the genetic stable rates of pMG36e in E. coli JM109 and Lactococcus lactis MG1363/36e with or without erythomycin selective pressure.


RESULTS
When E. coli JM109 were continuously incubated to 120 generations, its pMG36e genetic stable rates were 100% and 96% respectively with or without erythomycin selective pressure. When Lactococcus lactis MG1363/36e were continuously incubated to 100 generations, its pMG36e genetic stable rates were the same 98%, with or without erythomycin selective pressure.


CONCLUSIONS
pMG36e had good genetic stability in this two hosts. Erythromycin selective pressure had no significant effect for the genetic stability of pMG36e.
Wang
Chuan
C
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Chao-wu
CW
Du
Jiang
J
Lü
Xiao-ying
XY
D003470:Culture Media; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D012692:Serial Passage
D003470:Culture Media; D004917:Erythromycin