[Effects of combined naloxone and methylprednisolone on nuclear factor-kappaB expression in the lung in acute lung injury in rats].
OBJECTIVE
To investigate the effects of a combination of naloxone and methylprednisolone on nuclear factor-kappaB (NF-kappaB) p65 expression in the lung tissue in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats.


METHODS
ALI models were reproduced by intratracheal instillation of LPS (3 mg/kg). Four hours after LPS instillation, rats were randomly divided into five groups: normal saline group, LPS group, methylprednisolone group, naloxone group (LPS+naloxone) and combined drug group (LPS+naloxone+methylprednisolone). The level of interleukin-8 (IL-8) in serum was measured by immunoassay. Meanwhile, the expression of NF-kappaB p65 in the lung tissue was determined with immunohistochemical staining.


RESULTS
Naloxone and methylprednisolone significantly reduced the LPS-induced increase in IL-8 concentrations in serum in vivo, and suppressed the activation of NF-kappaB p65 in the lung tissue.


CONCLUSIONS
NF-kappaB activation is involved in the LPS-induced ALI in rats. Combination of naloxone and methylprednisolone could suppress the increase of IL-8 content and NF-kappaB activation in the lung tissue of rat in vivo in our experiment.
Zhang
Qiu-jin
QJ
Emergency Department, General Hospital of PLA, Beijing 100853, China.
Shen
Hong
H
Zhang
Wei
W
Li
Yin-ping
YP
Li
Tan-shi
TS
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D016209:Interleukin-8 / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009270:Naloxone; D008775:Methylprednisolone