An approach for genogrouping of Japanese isolates of aquabirnaviruses in a new genogroup, VII, based on the VP2/NS junction region.
Aquabirnaviruses, represented by Infectious pancreatic necrosis virus (IPNV), have been isolated from epizootics in salmonids and a variety of aquatic animals in the world; six genogroups of aquabirnaviruses have been identified. In comparisons of nucleotide sequences of the VP2/NS junction region, maximum nucleotide diversities of 30.8 % were observed among 93 worldwide aquabirnavirus isolates. A phylogenetic tree revealed the existence of a new genogroup, VII, for Japanese aquabirnavirus isolates from marine fish and molluscan shellfish. Nucleotide diversities between genogroups VII and I-VI were 18.7 % or greater. At the nucleotide level, Japanese IPNV isolates from epizootics in salmonids were nearly identical to a genogroup I strain from the USA or Canada. It is suggested that Japanese IPNV isolates belonging to genogroup I were originally introduced from North American sources, whereas Japanese aquabirnavirus isolates of genogroup VII were from marine aquatic animals indigenous to Japan.
Nishizawa
Toyohiko
T
Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
Kinoshita
Shinichi
S
Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
Yoshimizu
Mamoru
M
Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
D000818:Animals; D019201:Aquabirnavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics; D005393:Fish Diseases / Q000821:virology*; D005399:Fishes / Q000821:virology*; D018061:Infectious pancreatic necrosis virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012487:Salmonidae / Q000821:virology; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D017361:Viral Nonstructural Proteins