Phosphatidylinositol biosynthesis: biochemistry and regulation.
Phosphatidylinositol (PI) is a ubiquitous membrane lipid in eukaryotes. It is becoming increasingly obvious that PI and its metabolites play a myriad of very diverse roles in eukaryotic cells. The Saccharomyces cerevisiae PIS1 gene is essential and encodes PI synthase, which is required for the synthesis of PI. Recently, PIS1 expression was found to be regulated in response to carbon source and oxygen availability. It is particularly significant that the promoter elements required for these responses are conserved evolutionarily throughout the Saccharomyces genus. In addition, several genome-wide strategies coupled with more traditional screens suggest that several other factors regulate PIS1 expression. The impact of regulating PIS1 expression on PI synthesis will be discussed along with the possible role(s) that this may have on diseases such as cancer.
Gardocki
Mary E
ME
Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit MI 48202, USA.
Jani
Niketa
N
Lopes
John M
JM
D005057:Eukaryotic Cells / Q000201:enzymology; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D010641:Phenotype; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism*
D010716:Phosphatidylinositols; D029701:Saccharomyces cerevisiae Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C478389:PIS1 protein, S cerevisiae