An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging.
An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540 nm with a peak intensity of around 490 nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation.
Martin
Matt E
ME
Center for Advanced Biomedical Photonics, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, USA.
Wabuyele
Musundi
M
Panjehpour
Masoud
M
Overholt
Bergein
B
DeNovo
Robert
R
Kennel
Steve
S
Cunningham
Glenn
G
Vo-Dinh
Tuan
T
D000818:Animals; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007834:Lasers; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration