Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain.
Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids beta-(1,4) linked and variably C2 and/or C3O-acetylated. To obtain beta-Delta-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using 1H NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan.
Delattre
C
C
Laboratoire des Glucides-EPMV CNRS FRE 2779, IUT/GB, UPJV, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex, France.
Michaud
P
P
Lion
J M
JM
Courtois
B
B
Courtois
J
J
D000107:Acetylation; D002853:Chromatography, Liquid; D005656:Fungal Proteins / Q000737:chemistry*; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D016962:Sinorhizobium meliloti / Q000737:chemistry* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
D005656:Fungal Proteins; D005965:Glucuronates; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase