Expression and characterization of the intracellular vanilloid receptor (TRPV1) in bronchi from patients with chronic cough.
TRPV1 is a modulator of noxious stimuli known to be important in the cough reflex. We have compared the expression of TRPV1 in normal human airways and those from patients with chronic cough and found that there is up regulation in airways smooth muscle in disease. This increased expression appears to be intracellular and we have therefore examined the role of intracellular rat and human TRPV1 activity was found using intracellular calcium signalling with human intracellular TRPV1 being located in a thapsigargin insensitive compartment. Increase in TRPV1 activity may have a role in the airway hypersensitivity seen in chronic cough.
Mitchell
Jennifer E
JE
Department of Cellular Pathology, Hull Royal Infirmary, Hull, United Kingdom.
Campbell
Anne P
AP
New
Norman E
NE
Sadofsky
Laura R
LR
Kastelik
Jack A
JA
Mulrennan
Siobhan A
SA
Compton
Steven J
SJ
Morice
Alyn H
AH
D000818:Animals; D001980:Bronchi / Q000378:metabolism* / Q000473:pathology; D016535:Bronchial Hyperreactivity / Q000235:genetics / Q000378:metabolism; D001991:Bronchitis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020013:Calcium Signaling; D016022:Case-Control Studies; D002460:Cell Line; D002908:Chronic Disease; D003371:Cough / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology; D007424:Intracellular Fluid / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats; D050916:TRPV Cation Channels
D007473:Ion Channels; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C493922:Trpv1 protein, rat