title: Microvascular density, vascular endothelial growth factor immunoreactivity in tumor cells, vessel diameter and intussusceptive microvascular growth in primary melanoma.
abstract: Intussusceptive microvascular growth (IMG) corresponds to one of the types of angiogenesis described in literature. Recent morphological work strongly supports a role for IMG, even during tumor angiogenesis. In this study, the extent of angiogenesis, evaluated as microvascular density, the immunoreactivity of tumor cells to vascular endothelial growth factor (VEGF), the vessel diameter and the IMG have been correlated to the tumor thickness in human primary melanoma specimens. Results showed that an increased microvascular density, a strong VEGF immunoreactivity of tumor cells, a major vessel diameter and a high number of connections of intraluminal tissue folds with the opposite vascular wall, expression of IMG, are correlated to a high tumor thickness (>3.6 mm). Overall, these data demonstrate for the first time in human primary melanoma a relationship between angiogenesis, VEGF immunoreactivity of tumor cells, vessels diameter and IMG and seem to indicate that VEGF is specifically involved in increasing vessel diameter and IMG.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, 11 Piazza Giulio Cesare, Policlinico, I-70124 Bari, Italy. ribatti@anatomia.uniba.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Carlo
authors.initials: C
authors.lastname: Floris
authors.forename: Domenica
authors.initials: D
authors.lastname: Mangieri
authors.forename: Franca
authors.initials: F
authors.lastname: Piras
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Ennas
authors.forename: Angelo
authors.initials: A
authors.lastname: Vacca
authors.forename: Paola
authors.initials: P
authors.lastname: Sirigu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D012878:Skin Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A