Direct interaction between SNAP-23 and L-type Ca2+ channel.
During secretion, membrane-bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca2+-channels. Ca2+-channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t-SNARE proteins, Syntaxin-1 and SNAP-25 with various Ca2+-channels. In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+-channel at the plasma membrane in pancreas.
Cho
Won Jin
WJ
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
Jeremic
Aleksandar
A
Jena
Bhanu P
BP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D047468:Immunoprecipitation; D008297:Male; D008958:Models, Molecular; D010179:Pancreas / Q000737:chemistry; D011485:Protein Binding / Q000502:physiology; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D020746:Calcium Channels, L-Type; D002352:Carrier Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human