title: [Selective heterologous communication among human lung epithelial cells, fibroblasts, and lung cancer PG cells].
abstract: OBJECTIVE
  To further understand the roles of gap junctional intercellular communication in carcinogenesis and progression of human lung cancer.
  
  
  METHODS
  The heterologous communication was characterized among human lung epithelial cells HLEC, human lung fibroblasts HLF, human lung giant carcinoma PG cells and its Cx43 transfectants PG/C4 by using a preloading assay. Cx43 immunofluorescent staining and Northern blot were performed to examine Cx43 gene expression.
  
  
  RESULTS
  Although both human lung fibroblasts HLF and epithelial cells HLEC expressed Cx43 and had very strong homologous communication, HLEC cells were unable to communicate with HLF cells. The human lung carcinoma cell line PG was defective of both homologous communication, and heterologous communication with its epithelial origin HLEC. Transfection of Cx43 into PG cells, which rescued PG cells' homologous communication and enhanced heterologous communication with HLF cells, could restore heterologous coupling with HLEC cells.
  
  
  CONCLUSIONS
  Selective heterologous communication exists among different kinds of human lung cells, when human lung carcinoma cells lost coupling with its epithelial origin, Cx43 gene expression is not enough to establish heterologous communication between them.
authors.affiliation: Department of Cell Biology, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100034, China. zqzhang@public3.bta.net.cn
authors.forename: Zhi-qian
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
mesh_terms: D002450:Cell Communication; D018031:Connexin 43 / Q000096:biosynthesis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000473:pathology*
chemical_list: D018031:Connexin 43