Exogenous hyaluronic acid enhances porcine parthenogenetic embryo development in vitro possibly mediated by CD44.
Exogenous hyaluronic acid (HA) has been reported to improve early embryo development in vitro in pigs and cows. Although early embryo development in vitro is improved by exogenous HA, the mechanism mediating the action of HA is not clearly defined. In the present study, two possible HA actions on early embryo development were proposed to understand interactions between HA and the embryos using porcine parthenotes. We hypothesized that improvement of early embryo development mediated by HA would be caused by embryo-derived growth factors due to the high molecular weight of HA or cellular response through its receptor (CD44). We examined the effects of HA molecular weight on parthenogenetic embryo development, permeability of HA into the zona pellucida, expression of CD44 in porcine parthenotes at various stages, and blocking interactions between HA and CD44 by monoclonal anti-CD44 antibody (mCD44Ab). As a result, although development of porcine parthenotes to the blastocyst stage was significantly enhanced by exogenous HA with various molecular weights, there was no difference in blastocyst formation among the various molecular weights (P < 0.05). Immunofluorescence revealed that exogenous HA was accessible to CD44 through the zona pellucida, irrespective of the oocyte activation and that CD44 was also expressed in both oocytes and parthenotes at all developmental stages. In addition, development of parthenotes was partially blocked by mCD44Ab. In conclusion, we demonstrated that exogenous HA enhanced development of porcine parthenotes in vitro. This improvement mediated by exogenous HA on parthenogenetic embryo development was possibly caused by cellular response via CD44.
Toyokawa
K
K
Department of Life Science, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Hyogo, Japan. toyo7394@uidaho.edu
Harayama
H
H
Miyake
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000502:physiology; D002463:Cell Membrane Permeability; D003571:Cytochalasin B / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D018960:Hyaluronan Receptors / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D008970:Molecular Weight; D010312:Parthenogenesis / Q000187:drug effects*; D013552:Swine / Q000196:embryology*; D015044:Zona Pellucida / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D003571:Cytochalasin B; D006820:Hyaluronic Acid