The relative levels of cyclin D1a and D1b alternative transcripts in mantle cell lymphoma may depend more on sample origin than on CCND1 polymorphism.
The relative levels of cyclin D1 (CCND1) (a) and (b) transcripts were determined by real-time reverse transcription polymerase chain reaction (RT-PCR) and found to vary according to the tissue origin in both control and tumor samples. A five-fold overexpression of both isoforms was observed in 28/38 cases of mantle cell lymphoma (MCL) and of only one isoform in 10/38 MCL. No correlation was observed between expression of cyclin D1 isoforms and CCND1 genotype at position 870.
Carrère
Nathalie
N
Belaud-Rotureau
Marc-Antoine
MA
Dubus
Pierre
P
Parrens
Marie
M
de Mascarel
Antoine
A
Merlio
Jean-Philippe
JP
D017398:Alternative Splicing; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000378:metabolism*; D011110:Polymorphism, Genetic; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020033:Protein Isoforms; D019938:Cyclin D1