title: F-actin, beta-tubulin, aldolase, and fructose-1,6-bisphosphatase in heteropteran ovarioles--I. Immunocytochemical investigations of whole-mounted ovarioles.
abstract: The distribution of F-actin, beta-tubulin, aldolase, and fructose-1,6-bisphosphatase (FBPase) in ovarioles of four heteropteran species (Ilyocoris cimicoides, Coreus marginatus, Lygus pratensis, and Notostira elongata) was investigated biochemically and immunocytochemically. Aldolase was found to be uniformly distributed in the cytoplasm of trophocytes and follicular cells, with the highest concentration in prefollicular cells. Its concentration in follicular cells increased during differentiation and reached a peak in ovarian follicles at the stage of late choriogenesis. FBPase was observed in the cytoplasm (weak reaction) and on cell borders (strong reaction) of both germ line and somatic cells. No FBPase or aldolase signal was observed on the F-actin trophic core mesh or on stress fibers.
authors.affiliation: Zoological Institute, Wrocław University, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Dziewulska-Szwajkowska
authors.forename: Antoni
authors.initials: A
authors.lastname: Ogorzałek
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D005854:Germ Cells / Q000378:metabolism / Q000648:ultrastructure; D020062:Heteroptera / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D022502:Stress Fibers / Q000378:metabolism / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000199:Actins; D014404:Tubulin; D006597:Fructose-Bisphosphatase; D005634:Fructose-Bisphosphate Aldolase