The placental RCAS1 expression during stillbirth.
BACKGROUND
Independently of the fetal death cause the beginning and course of stillbirth is closely related with the growing cytotoxic activity at the maternal-fetal interface. RCAS1 participates in the inhibition of maternal immune response during pregnancy. The alterations of RCAS1 protein expression in placental cells seem to determine the beginning of the labor and participate in the placental abruption. The aim of the present study was to investigate RCAS1 expression in placentas obtained following stillbirths or normal term births.


METHODS
RCAS1 expression was evaluated by Western blot method with the use of monoclonal anti-RCAS1 antibody in 67 placental tissue samples. Pregnant women were divided into four groups according to the mode of labor onset--spontaneous or induced, and the type of labor, stillbirth or labor at term. Placental beta-Actin expression was chosen as a control protein. Relative amounts of placental RCAS1 were compared with the use of Student's t-test, whereas beta-Actin control data were compared with the use of Mann-Whitney U test.


RESULTS
The average relative amount of RCAS1 was significantly lower in women with induced stillbirths than in women with induced labor at term. Similarly, significantly lower RCAS1 placental levels were observed in patients with spontaneous stillbirths than in women with spontaneous labor at term. Significant differences in RCAS1 expression were also observed with the respect to the beginning of the stillbirth: spontaneous and induced. Lowest RCAS1 placental levels were observed in women with spontaneous stillbirth.


CONCLUSIONS
These preliminary results indicate that the alterations of RCAS1 expression in the human placenta may be involved in the changes of maternal immune system that take place during stillbirth.
Wicherek
Lukasz
L
Department of Gynecology and Infertility, Jagiellonian University, 23 Kopernik Str, 31-501 Krakow, Poland. mowicher@cyf-kr.edu.pl
Klimek
Marek
M
Czekierdowski
Artur
A
Popiela
Tadeusz J
TJ
Galazka
Krystyna
K
Tetlak
Tomasz
T
Gilowski
Andrzej
A
Dutsch-Wicherek
Magdalena
M
D000328:Adult; D000951:Antigens, Neoplasm / Q000378:metabolism*; D005260:Female; D006801:Humans; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D050497:Stillbirth / Q000453:epidemiology
D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human