title: Gene expression profile in oral squamous cell carcinoma: a pilot study.
abstract: OBJECTIVE
  To study the gene expression profile of oral squamous cell carcinomas.
  
  
  METHODS
  Gene expression profile was investigated in oral squamous cell carcinomas in 5 patients using the Atlas Glass Human 3.8 I Microarray (which detects cDNA obtained from cellular total RNA) (Clontech Laboratories, Palo Alto, CA). Data were normalized by the LOWESS method. Statistical significances of deviations from a 1:1 ratio were evaluated by t tests, with P<.05.
  
  
  RESULTS
  Of the 3,757 genes analyzed, 322 (8.6%) were significantly overexpressed in tumoral tissue with respect to normal tissue, while 104 (2.8%) were significantly underexpressed. The affected genes fell into a wide range of functional categories.
  
  
  CONCLUSIONS
  We consider that cDNA microarrays are of clear value for investigating the biology of these tumors, and that this technology may help in the molecular classification of oral squamous cell carcinomas and in the identification of targets for gene therapy.
authors.affiliation: Universidad de Santiago de Compostela, Complejo Hospitalario, Universitario de Santiago, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Somoza-Martín
authors.forename: Abel
authors.initials: A
authors.lastname: García-García
authors.forename: Francisco
authors.initials: F
authors.lastname: Barros-Angueira
authors.forename: Eva
authors.initials: E
authors.lastname: Otero-Rey
authors.forename: María
authors.initials: M
authors.lastname: Torres-Español
authors.forename: Pilar
authors.initials: P
authors.lastname: Gándara-Vila
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Reboiras-López
authors.forename: Andrés
authors.initials: A
authors.lastname: Blanco-Carrión
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Gándara-Rey
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects
chemical_list: D004273:DNA, Neoplasm