Molecular characterization of crane Coccidia, Eimeria gruis and E. reichenowi, found in feces of migratory cranes.
Eimeria gruis and E. reichenowi have lethal pathogenicity to a number of species of cranes. These parasites develop at multiple organs or tissues in infected cranes, thus lacking the specificity of infection sites shown by other Eimeria spp. in spite of morphologic similarity. To date, there have been many reports of crane Eimeria infections, however, genetic examinations of these parasites have never been published. In the present study, we isolated oocysts of E. gruis and E. reichenowi from crane feces at a wintering area in Japan. By phylogenic analysis, we first demonstrated that partial sequences of the isolates formed their own cluster, located separately from other Eimeria spp.
Matsubayashi
Makoto
M
Department of Food and Nutrition, Osaka Yuhigaoka Gakuen Junior College, Tennoji-ku, Osaka 543-0073, Japan.
Takami
Kazutoshi
K
Abe
Niichiro
N
Kimata
Isao
I
Tani
Hiroyuki
H
Sasai
Kazumi
K
Baba
Eiichiroh
E
D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D001715:Bird Diseases / Q000469:parasitology*; D001717:Birds / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology* / Q000662:veterinary*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004539:Eimeria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D007564:Japan; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D033661:Oocysts / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S