Identification and quantitation of eleven sesquiterpenes in three species of Curcuma rhizomes by pressurized liquid extraction and gas chromatography-mass spectrometry.
In this paper, GC-MS and pressurized liquid extraction (PLE) was developed for identification and quantitative determination/estimation 11 sesquiterpenes including germacrene D, curzerene, gamma-elemene, furanodienone, curcumol, isocurcumenol, furanodiene, germacrone, curdione, curcumenol and neocurdione in Ezhu which are derived from three species of Curcuma, i.e., Curcuma phaeocaulis, Curcuma wenyujin and Curcuma kwangsiensis by using an analogue as standard. The results showed the methodology could quantitatively compare the quality of three species of Curcuma. The contents of investigated sesquiterpenes in three species of Curcuma were high variant. Hierarchical clustering analysis based on characteristics of 11 identified peaks in GC profiles showed that 18 samples were divided into two main clusters, C. phaeocaulis and C. wenyujin, respectively. C. kwangsiensis showed the characters closed to C. phaeocaulis or C. wenyujin based on its location. Five components such as furanodienone, germacrone, curdione, curcumenol and neocurdione were optimized as markers for quality control of Ezhu.
Yang
F Q
FQ
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China.
Li
S P
SP
Chen
Y
Y
Lao
S C
SC
Wang
Y T
YT
Dong
Tina T X
TT
Tsim
Karl W K
KW
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D030024:Curcuma / Q000378:metabolism*; D004345:Drug Industry / Q000379:methods*; D005663:Furans / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006574:Heterocyclic Compounds, 2-Ring / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010802:Phylogeny; D011786:Quality Control; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry*; D045788:Sesquiterpenes, Germacrane / Q000032:analysis
D005663:Furans; D006574:Heterocyclic Compounds, 2-Ring; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C504126:curdione; C038905:elemene; C442489:furanodiene; C048393:germacrone; C022801:curcumol; C459499:isocurcumenol; C027259:germacrene D