In vitro cytokine profile in childhood alopecia areata and the immunomodulatory effects of AS-101.
The aim of the present study was to determine the effect of AS-101, a known immunomodulator, on the pattern of cytokine production in children with patchy alopecia areata (PAA). Ten previously untreated children with PAA were compared to 10 healthy age- and sex-matched controls. Peripheral blood mononuclear cells (PBMC) were isolated from all participants. Unstimulated and phytohaemagglutinin (PHA)-stimulated PBMC were tested with and without the addition of AS-101. The production of interferon gamma (IFNgamma), soluble interleukin (IL)-2 receptor (IL-2R), IL-10, IL-5 and IL-6 was determined. The levels of soluble IL-2R, IL-5 and IL-6 were significantly higher in the PAA patients than the controls. AS-101 inhibited the production of IL-10, IFNgamma, IL-2R and IL-5 in both PAA patients and controls, but there was a greater inhibitory effect in children with PAA.
Shohat
M
M
Department of Dermatology and Cellular Immunology Unit, Rabin Medical Center, Beilinson Campus, Petah Tiqva, Israel.
Mimouni
D
D
Ben-Amitai
D
D
Sredni
B
B
Sredni
D
D
Shohat
B
B
David
M
M
D000293:Adolescent; D000506:Alopecia Areata / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D010835:Phytohemagglutinins / Q000276:immunology
D016207:Cytokines; D005030:Ethylenes; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; C054105:ammonium trichloro(dioxoethylene-O,O'-)tellurate