Determination of total N-nitroso compounds by chemical denitrosation using CuCl.
A method for the determination of total N-nitroso compounds (NOC) by chemical denitrosation and subsequent chemiluminescence detection of evolved NO is described. Denitrosation was accomplished with CuCl in HCl at 70 degrees C. The detection limit for N-nitrosoproline (NPRO) was 1 pmol. NO formation from NPRO was linear (R(2) = 0.999) from 4 pmol to 2 nmol. Among the possible interfering compounds tested, only S-nitroso compounds contribute any significant interference. This method had several advantages over other similar methods: (1) A commercially available one-piece reaction vessel and a NO analyzer with software were used. (2) NO release occurred rapidly and was easily measured and quantified. (3) Compared to HBr or HI, CuCl was more convenient to work with and safe. (4) CuCl was suitable for samples in aqueous and most organic solvents. The application of this method to food, personal care products, and human body fluids demonstrates its utility.
Wang
Jia
J
Philip Morris USA Research Center, 4201 Commerce Road, Richmond Virginia 23234, USA.
Chan
W Geoffrey
WG
Haut
Stephen A
SA
Krauss
Marc R
MR
Izac
Richard R
RR
Hempfling
Walter P
WP
D003300:Copper / Q000737:chemistry*; D006851:Hydrochloric Acid; D008163:Luminescent Measurements; D009569:Nitric Oxide / Q000032:analysis / Q000737:chemistry; D009603:Nitroso Compounds / Q000032:analysis*
D009603:Nitroso Compounds; D009569:Nitric Oxide; D003300:Copper; D006851:Hydrochloric Acid; C029892:cupric chloride