Comparative study of antioxidants as quenchers or scavengers of reactive oxygen species based on quenching of MCLA-dependent chemiluminescence.
The quenching or scavenging effect of non-enzymatic antioxidants against reactive oxygen species (ROS) was studied by comparing the degree of suppression of chemiluminescence (CL) caused by the oxidation of MCLA (methoxylated Cypridina luciferin analogue) by ROS. MCLA-dependent CL caused by O2- was effectively quenched by ascorbic acid, beta-carotene, lycopene and astaxanthin, while it was enhanced by alpha-tocopherol. The CL by 1O2 was quenched effectively by beta-carotene, lycopene and astaxanthin, moderately by ascorbic acid, and slightly by alpha-tocopherol. beta-Carotene and alpha-tocopherol remarkably suppressed the CL when ROS was HO*. The present study revealed that MCLA-dependent CL assay provides a simple and rapid method for the evaluation of antioxidants as a quencher or scavenger against any kind of ROS.
Hosaka
S
S
Department of Applied Chemistry, Faculty of Engineering, Tokyo Polytechnic University, 1583 Atsugi, Kanagawa 243-0297, Japan. s-hosaka@parkcity.ne.jp
Obuki
M
M
Nakajima
J
J
Suzuki
M
M
D000975:Antioxidants / Q000737:chemistry*; D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D049449:Luminescence; D015394:Molecular Structure; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012680:Sensitivity and Specificity; D026082:Singlet Oxygen / Q000737:chemistry; D013997:Time Factors
D000975:Antioxidants; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D049454:Firefly Luciferin