title: Screening for ZNF230 gene mutation and analysis of its correlation with azoospermia.
abstract: OBJECTIVE
  To investigate the possible association between ZNF230 gene and azoospermia.
  
  
  METHODS
  Screening for mutation of all 6 exons of ZNF230 gene was performed by denaturing high performance liquid chromatography(DHPLC) in 99 patients with azoospermia and in 115 healthy men as controls.
  
  
  RESULTS
  An A-->G transition at nucleotide 316 in exon 6 was identified. There were significant differences in the distribution profiles of both allele and genotype frequencies between patient group and control group (P < 0.01 and P < 0.05, respectively). In addition,there was a statistically significant difference in the serum follicle stimulating hormone (FSH) level between the patients with GG/GA genotype and those with AA genotype (P < 0.05).
  
  
  CONCLUSIONS
  ZNF230 gene may be associated with azoospermia, and the A316G mutation may be correlated with the serum FSH level.
authors.affiliation: Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
authors.forename: Jing-tao
authors.initials: JT
authors.lastname: Dong
authors.forename: Si-zhong
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Ma
authors.forename: Kai-xuan
authors.initials: KX
authors.lastname: Yang
authors.forename: Ming-kong
authors.initials: MK
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Guo-ping
authors.initials: GP
authors.lastname: He
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000175:diagnosis / Q000235:genetics*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors; C440989:ZNF230 protein, human