[Determination of HLA-A, B, and DRB1 gene polymorphism in Monba ethnic group of Xizang Autonomous Region, China].
OBJECTIVE
To investigate the HLA-A, B, DRB1 allele polymorphism in Monba ethnic population of Xizang Autonomous Region.


METHODS
HLA-A, B, DRB1, DNA types in 47 unrelated Monba ethnic healthy individuals from the Xizang Autonomous Region of China were analyzed by polymerase chain reaction-based reverse line-strip sequence specific oligonucleotide hybridization. Based on the HLA-DRB1 alleles frequencies of 11 other ethnic groups in China, a molecular phylogenetic tree was constructed by the Neighbor-Joining (NJ) method.


RESULTS
Twenty-three alleles were detected for HLA-A, 39 for HLA-B, and 33 for HLA-DRB1. HLA-A*1101, A*2402, A*02011, A*0206, HLA-B*3802, B*4001, B*4002, B*51011, HLA-DRB1*12021, DRB1*0403, DRB1*0701, DRB1*1201 were the most common alleles. The most frequent alleles were HLA-A*1101(0.2128), HLA-B*3802(0.1064), and HLA-DRB1*12021(0.0851).


CONCLUSIONS
The HLA loci are highly polymorphic in Monba population of Xizang Autonomous Region. Compared with other ethnic groups in China, the Monba ethnic group is close to Tibetan ethnic group.
Zhang
Hong-bo
HB
National Ministry of Health for Forensic Sciences and Environment and Genes Related to Diseases, Key Laboratory of the Education Ministry, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China.
Gao
Fang
F
Kang
Long-li
LL
Li
Sheng-bin
SB
D000483:Alleles; D002681:China; D005006:Ethnicity / Q000145:classification / Q000235:genetics*; D005787:Gene Frequency; D006680:HLA Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D006680:HLA Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains