title: Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.
abstract: Dectin-2 is a recently described dendritic-cell-associated receptor, suggested to be involved in the initiation and maintenance of UV-induced tolerance. To understand the physiological relevance of the proposed functions of this C-type lectin-like receptor, we have generated monoclonal antibodies against its extracellular domain and performed a detailed study of its expression. In naive mice, Dectin-2 has a novel distribution pattern compared with other myeloid markers, but is predominantly expressed by a wide variety of tissue macrophages. Its expression was limited on dendritic cells and notably absent from brain microglia and choroid plexus or meningeal macrophages. On peripheral blood monocytes, Dectin-2 expression was very low on the surface but was transiently and markedly up-regulated on induction of inflammation in vivo using a variety of stimuli. This change in Dectin-2 expression occurs on 'inflammatory' monocytes after arrival at the inflammatory lesion as demonstrated by adoptive cell-transfer studies, and is independent of whether the macrophages elicited by the stimuli ultimately expressed Dectin-2. These observations show Dectin-2 expression to be characteristic of monocyte activation/maturation at an inflammatory lesion and provide a new perspective on the interpretation of Dectin-2 function in vivo.
authors.affiliation: Sir William Dunn School of Pathology, Oxford University, Oxford, UK. philip.taylor@path.ox.ac.uk
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Delyth M
authors.initials: DM
authors.lastname: Reid
authors.forename: Sigrid E M
authors.initials: SE
authors.lastname: Heinsbroek
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Brown
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
authors.forename: Simon Y C
authors.initials: SY
authors.lastname: Wong
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D007150:Immunohistochemistry; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology* / Q000637:transplantation; D022423:Myeloid Cells / Q000378:metabolism* / Q000473:pathology* / Q000637:transplantation; D041681:NIH 3T3 Cells; D014161:Transduction, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D037181:Lectins, C-Type; C445340:dectin-2, mouse