title: Enhanced clearance of Candida albicans from the oral cavities of mice following oral administration of Lactobacillus acidophilus.
abstract: Orally administered live Lactobacillus acidophilus was assessed for its capacity to enhance clearance from the oral cavity of DBA/2 mice shown previously to be 'infection prone'. L. acidophilus fed to DBA/2 mice significantly shortened the duration of colonization of the oral cavity compared to controls. Enhanced clearance of Candida albicans correlated with both early mRNA gene expression for interleukin (IL)-4 and interferon (IFN)-gamma and expression of their secreted products in cultures of cervical lymph nodes stimulated with Candida antigen. In addition rapid clearance correlated with higher levels of IFN-gamma and nitric oxide in saliva. Delayed clearance, less pronounced levels of the cytokine response, saliva IFN-gamma and nitric oxide, and later mRNA expression for IL-4 and IFN-gamma relative to feeding with the L. acidophilus isolate were noted in mice fed a different Lactobacillus isolate (L. fermentum). These observations indicate significant variations in individual isolates to activate the common mucosal system.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Sasketchewan, Sasketoon, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Elahi
authors.forename: G
authors.initials: G
authors.lastname: Pang
authors.forename: R
authors.initials: R
authors.lastname: Ashman
authors.forename: R
authors.initials: R
authors.lastname: Clancy
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002176:Candida albicans / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D018928:Immunity, Mucosal; D007779:Lactobacillus acidophilus; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009055:Mouth / Q000382:microbiology*; D009333:Neck; D009569:Nitric Oxide / Q000096:biosynthesis; D019936:Probiotics / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000276:immunology
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D009569:Nitric Oxide