title: Dose-dependent inhibition of gynecophoral canal protein gene expression in vitro in the schistosome (Schistosoma japonicum) by RNA interference.
abstract: The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose-dependent. RT-PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied.
authors.affiliation: Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Cheng
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.forename: You-Xin
authors.initials: YX
authors.lastname: Jin
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Jin
authors.forename: Yuan-Cong
authors.initials: YC
authors.lastname: Zhou
authors.forename: You-Min
authors.initials: YM
authors.lastname: Cai
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis*; D015801:Helminth Proteins / Q000096:biosynthesis*; D008297:Male; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal
chemical_list: D006023:Glycoproteins; D015801:Helminth Proteins; D012330:RNA, Double-Stranded; C103128:gynecophoral canal protein, 86-kDa