title: Identification and expression of a new type II transmembrane protein in human mast cells.
abstract: A cDNA encoding a new type II transmembrane protein has been isolated from human mast cells by subtraction cloning. This cDNA contains an open reading frame of 186 amino acids. RT-PCR analysis showed that this gene is differentially expressed in mast cells. Therefore, the peptide encoded by this gene was termed mast cell-expressed membrane protein 1 (MCEMP1). The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. Monoclonal antibodies against MCEMP1 were generated and characterized by immunoprecipitation and FACS. The results showed that the native MCEMP1 is expressed in cord blood-derived mast cells and HMC-1 and THP-1 cell lines, but not in other cell types that we have tested. Immunochemical staining of human lung sections showed that MCEMP1 staining is specifically associated with lung mast cells.
authors.affiliation: Tanox, Inc., 10301 Stella Link Road, Suite 110, Houston, TX 77025, USA. kli@tanox.com
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Shen-Wu
authors.initials: SW
authors.lastname: Wang
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Li
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Martin
authors.forename: Lixin
authors.initials: L
authors.lastname: Li
authors.forename: Meisheng
authors.initials: M
authors.lastname: Lu
authors.forename: Salah-Eddine
authors.initials: SE
authors.lastname: Lamhamedi-Cherradi
authors.forename: Guanghui
authors.initials: G
authors.lastname: Hu
authors.forename: Sossiena
authors.initials: S
authors.lastname: Demissie-Sanders
authors.forename: Ji
authors.initials: J
authors.lastname: Zheng
authors.forename: Fan
authors.initials: F
authors.lastname: Chung
authors.forename: Tim
authors.initials: T
authors.lastname: Oates
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Yao
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; C506757:MCEMP1 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins