[Effects of alcohol on the expression of heat shock protein 70 and super microstructure of astrocytes in rat embryonic brain].
OBJECTIVE
To explore effects of alcohol on the expression of heat shock protein 70 (HSP70) and super microstructure of astrocytes.


METHODS
Different dosage of alcohol (1, 5, 25, 50mmol/L) was administered to astrocytes of rat embryos. Effects of alcohol on expression of HSP70 were studied by immunocytochemistry, and changes of super microstructure of astrocytes were observed by electron microscope.


RESULTS
The results showed that with increase of alcohol dosage, the expression of HSP70 increased, hepatin granule and survival astrocytes in 25mmol/L and 50mmol/L dosage group decreased, cell membrane and mitochondrion were damaged, and even apoptosis and necrosis occurred.


CONCLUSIONS
It suggested that alcohol could increase expression of HSP of astrocytes, which could depress the expression of normal function protein, and mainly impaired cell membrane and mitochondrion, which could lead to apoptosis and necrosis.
Wang
Xia
X
School of Public Health, Fudan University, Shanghai 200032, China.
Qu
Wei-dong
WD
Ya
Seng
S
Xia
Tian
T
Zhang
Tianbao
T
Jiang
Songhui
S
Zhu
Huigang
H
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D018840:HSP70 Heat-Shock Proteins; D000431:Ethanol