title: Slit/Robo signaling is necessary to confine early neural crest cells to the ventral migratory pathway in the trunk.
abstract: Neural crest cells migrate along two discrete pathways within the trunk of developing embryos. In the chick, early migrating crest cells are confined to a ventral pathway medial to the dermamyotome while later cells migrate on a dorsal pathway lateral to the dermamyotome. Here we show that Slits are expressed in the dermamyotome, that early migrating crest cells express the Slit receptors Robo 1 and Robo 2, that Slit2 repels migrating crest cells in an in vitro assay, and that the misexpression of a dominant-negative Robo1 receptor induces a significant fraction of early crest cells to migrate ectopically in the dorso-lateral pathway. These findings suggest that Slits, most likely those expressed in the dermamyotome, help to confine the migration of early crest cells to the ventral pathway.
authors.affiliation: Department of Neuroscience, University of Pennsylvania School of Medicine, 1115, BRB II/III, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Jonathan
authors.initials: J
authors.lastname: Raper
mesh_terms: D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology*; D002642:Chick Embryo; D017931:DNA Primers; D029721:Drosophila Proteins / Q000378:metabolism*; D018274:Electroporation; D004625:Embryo, Nonmammalian / Q000196:embryology*; D015870:Gene Expression; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009432:Neural Crest / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D012160:Retina / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D000097905:Roundabout Proteins
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C066819:sli protein, Drosophila