[The preparation of fructose-modified chitosan microcarrier and culture of primary rat hepatocyte].
Crosslinked chitosan microcarries were prepared by the reaction of glutaraldehyde with fructose-modified chitosan. Various factors that influence the preparation were studied and the reaction conditions were optimized. Morphology of rat hepatocyte cultured on chitosan microcarriers was observed using phase contrast microscopy and scanning electron microscope, the metabolic activity was measured. Rat hepatocytes cultured on chitosan microcarrier retained the spherical shape as they have in vivo and had high metabolic activity. Fructose can enhance the metabolic activity of hepatocytes and fructose-modified chitosan microcarrier is a promising scaffold for hepatocytes attachment, which can be used in bioartificial liver support system.
Zhang
Li-Guo
LG
The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
Pan
Ji-Lun
JL
Li
Jie-Liang
JL
Yu
Yao-Ting
YT
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology* / Q000648:ultrastructure; D019164:Liver, Artificial; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D051381:Rats
D001672:Biocompatible Materials; D005632:Fructose; D048271:Chitosan