title: [Molecular cytogenetic analysis for a familial complex chromosomal rearrangement].
abstract: OBJECTIVE
  To determine a complex chromosomal rearrangement by advanced molecular cytogenetic techniques and analyze its clinical effect.
  
  
  METHODS
  A complex chromosomal rearrangement (CCR) involved in chromosomes 5, 16 and 20 in a 29-year-old male carrier was determined by chromosomal microdissection and multicolor fluorescence in situ hybridization (M-FISH), and family degree investigation was further performed.
  
  
  RESULTS
  The karyotype of the case was a complex chromosomal translocation among chromosomes 5, 20 and 16, and accompanied with a band of chromosome 20 inserted into chromosome 5. His mother and sister both had the same abnormal karyotype by familial investigation.
  
  
  CONCLUSIONS
  The combined use of M-FISH and chromosome microdissection is a powerful tool to determine CCR. The complex chromosomal rearrangement could be transmitted stably in the family, but still the carriers could give birth to a healthy baby by chance.
authors.affiliation: Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan, 410078, PR China.
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Qian
authors.forename: Yue-qiu
authors.initials: YQ
authors.lastname: Tan
authors.forename: Wai-mui
authors.initials: WM
authors.lastname: Tjia
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Xin-yuan
authors.initials: XY
authors.lastname: Guan
authors.forename: Guang-xiu
authors.initials: GX
authors.lastname: Lu
mesh_terms: D000328:Adult; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D011247:Pregnancy; D014178:Translocation, Genetic