Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags.
Phytophthora parasitica is a soilborne oomycete pathogen capable of infecting a wide range of plants, including many solanaceous plants. In a first step towards large-scale gene discovery, we generated expressed sequence tags (ESTs) from a cDNA library constructed using mycelium grown in synthetic medium. A total of 3568 ESTs were assembled into 2269 contiguous sequences. Functional categorization could be performed for 65.45% of ESTs. A significant portion of the transcripts encodes proteins of common metabolic pathways. The most prominent sequences correspond to members of the elicitin family, and enzymes involved in the lipid metabolism. A number of genes potentially involved in pathogenesis were also identified, which may constitute virulence determinants.
Panabières
Franck
F
INRA UMR 1064, Unité Interactions Plantes-Microorganismes et Santé Végétale, 400 route des Chappes, F-06930 Sophia-Antipolis cedex, France. panab@antibes.inra.fr
Amselem
Joëlle
J
Galiana
Eric
E
Le Berre
Jo-Yanne
JY
D000595:Amino Acid Sequence; D001483:Base Sequence; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D010802:Phylogeny; D010838:Phytophthora / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D020543:Proteome; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D005656:Fungal Proteins; D020543:Proteome