title: Novel integrated electrochemical nano-biochip for toxicity detection in water.
abstract: An electrochemical nano-biochip for water toxicity detection is presented. We describe chip design, fabrication, and performance. Bacteria, which have been genetically engineered to respond to environmental stress, act as a sensor element and trigger a sequence of processes, which leads to generation of electrical current. This novel, portable and miniature device provides rapid and sensitive real-time electrochemical detection of acute toxicity in water. A clear signal is produced within less than 10 min of exposure to various concentrations of toxicants, or to stress conditions, with a direct correlation between the toxicant concentration and the induced current.
authors.affiliation: Department of Electrical Engineering--Physical Electronics and TAU Research Institute for Nano Science and Nano-technologies, Tel-Aviv University. rachela@eng.tau.ac.il
authors.forename: Rachela
authors.initials: R
authors.lastname: Popovtzer
authors.forename: Tova
authors.initials: T
authors.lastname: Neufeld
authors.forename: Dvora
authors.initials: D
authors.lastname: Biran
authors.forename: Eliora Z
authors.initials: EZ
authors.lastname: Ron
authors.forename: Judith
authors.initials: J
authors.lastname: Rishpon
authors.forename: Yosi
authors.initials: Y
authors.lastname: Shacham-Diamand
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004777:Environment; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D015999:Multivariate Analysis; D036103:Nanotechnology / Q000379:methods*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; C489400:GrpE protein, E coli; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D014867:Water; C063842:dnaK protein, E coli