[Induction of protective immunity in rhesus monkey by inoculation with recombinant fusion protein of cholera toxin B subunit-multivalent epitopes of Plasmodium falciparum].
Rhesus monkeys (5 in each group) were inoculated with recombinant fusion protein of cholera toxin B subunit and multi-valent epitopes of Plasmodium falciparum intranasal or intramuscular (i.m.). Immune-responses and protective effect were evaluated. The antibody titer (Geometry mean) against CTB reached 1:512 (intranasal) and 1:10000 (i.m.) 14 day after 3rd immunization, and antibodies against P. falciparum were also elucidated, the titers in i.m. group were also significantly higher than that in intranasal group. The monkeys were challenged with 1.25 x 10(8) sporozoites of P. cynomolgi, Patent infection was observed in all 5 monkeys in control group inoculated with PBS in 10 - 14 days after challenge. Patent infection was also observed in 5 animals inoculated via intranasal and 2 animals in intramuscular group 19th days after challenge, But the infection last only 4 days in 3 animals in intranasal group and 2 animals in intramuscular group. The results demonstrated that the vaccine candidate could induce protective immune-responses in rhesus monkey against the challenge of P. cynomolgi.
Li
Ping
P
Beijing Institute of Biotechnology, Beijing 100850, China.
Zhong
Hui
H
Shi
Cheng-Hua
CH
Li
Jie-Zhi
JZ
Zhang
Yan-Hong
YH
Li
Chu-Fang
CF
Shi
Yun-Lin
YL
Ma
Qing-Jun
QJ
Cao
Cheng
C
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000913:Antibodies, Protozoan / Q000097:blood; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology*; D004912:Erythrocytes / Q000469:parasitology; D008253:Macaca mulatta; D008288:Malaria / Q000517:prevention & control / Q000662:veterinary*; D017780:Malaria Vaccines / Q000276:immunology*; D008992:Monkey Diseases / Q000517:prevention & control*; D016789:Plasmodium cynomolgi; D010963:Plasmodium falciparum / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D017780:Malaria Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D002772:Cholera Toxin