Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.
We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems.
Durany
Olga
O
Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
Bassett
Philip
P
Weiss
Amanda M E
AM
Cranenburgh
Rocky M
RM
Ferrer
Pau
P
López-Santín
Josep
J
de Mas
Carles
C
Hanak
Julian A J
JA
D000446:Aldehyde-Lyases / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D007218:Industrial Microbiology / Q000379:methods; D009875:Operator Regions, Genetic; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D012097:Repressor Proteins; D000446:Aldehyde-Lyases; C036027:L-fuculosephosphate aldolase