Electroporation-mediated transformation of Aeromonas hydrophila.
A strain of Aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as PHBHHx, was successfully transformed by electroporation. The plasmid used was a broad host range plasmid pBBR1MCS. Electroporation conditions were varied systemically to develop an electroporation protocol. The optimal yield of transformant was approximately 4x10(2) CFU/microg DNA at 12.5 kV/cm and 1000 Omega, resulting in a time constant of approximately 5 ms. The A. hydrophila transformants expressed plasmid-encoded resistance to chloromphenicol. Plasmid DNA in the A. hydrophila transformant was stably maintained. This is the first report of transformation of bacteria A. hydrophila.
Fengqing
Hu
H
Department of Life Science, Liaoning University, Shenyang 110036, China. drhfqing@hotmail.com
Song
You
Y
D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D002208:Caproates / Q000378:metabolism; D002702:Chloramphenicol Resistance / Q000235:genetics; D004269:DNA, Bacterial; D018274:Electroporation; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
D002208:Caproates; D004269:DNA, Bacterial; C045051:3-hydroxyhexanoic acid; D020155:3-Hydroxybutyric Acid