Protein mapping of the salivary complex from a hematophagous leech.
The salivary complex of leeches contains many components able to modulate physiological mechanisms, such as coagulation and fibrinolysis, and it is composed by the salivary glands and proboscis, encompassing two different proteomes. The bidimensional electrophoretic pattern of the salivary complex from the Haementeria depressa leech revealed a total of 352 spots, 103 in common with the muscular tissue and 249 exclusive from the salivary complex as detected by silver staining; these spots showed isoelectric points from 3.5 to 9.5 and covered an apparent molecular weight range from 10 to 105 kDa. The following isoforms of proteins were identified by mass spectrometry analysis: antiplatelet protein, myohemerythrin and carbonic anhydrase. Since the leeches were not fed for about 2-3 months to stimulate the secretion of proteins that facilitates the blood metabolism, these most abundant proteins in the salivary complex excised from leeches, are expected to play a role during feeding and might have some anti-hemostatic properties. Furthermore, by zymography, a gelatinolytic and a fibrinolytic protein were identified.
Ricci-Silva
Maria Esther
ME
Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, Brazil.
Konno
Katsuhiro
K
Faria
Fernanda
F
Rádis-Baptista
Gandhi
G
Fontes
Wagner
W
Stöcklin
Reto
R
Michalet
Sophie
S
Yamane
Tetsuo
T
Chudzinski-Tavassi
Ana Marisa
AM
D000595:Amino Acid Sequence; D000818:Animals; D002256:Carbonic Anhydrases / Q000235:genetics / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D006422:Hemerythrin / Q000235:genetics / Q000302:isolation & purification; D007865:Leeches / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012469:Salivary Glands / Q000737:chemistry / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006422:Hemerythrin; C073760:LAPP protein, Haementeria officinalis; D012471:Salivary Proteins and Peptides; C008571:myohemerythrin; D002256:Carbonic Anhydrases