PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species.
Two PCR assays have been developed to detect Aspergillus carbonarius and Aspergillus ochraceus, considered the main sources of ochratoxin A (OTA) contaminating commodities, particularly grapes, coffee and derivatives, in warm climates. The species specific primers have been designed on the basis of ITS (internal transcribed spacers of rDNA units) sequence comparisons obtained from Aspergillus strains and have been tested in a number of strains from different origins and hosts. These PCR assays, based on multi-copy sequences, are highly sensitive and specific and represent a good tool for an early detection of OTA-producing Aspergillus species and to prevent OTA entering the food chain.
Patiño
Belén
B
Departamento de Microbiología III, Universidad Complutense de Madrid, Spain.
González-Salgado
Amaia
A
González-Jaén
M  Teresa
M
Vázquez
Covadonga
C
D001230:Aspergillus / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D001235:Aspergillus ochraceus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009793:Ochratoxins / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D013045:Species Specificity
D004275:DNA, Ribosomal; D009793:Ochratoxins; C025589:ochratoxin A