title: Distribution and phenotype of murine rotavirus-specific B cells induced by intranasal immunization with 2/6 virus-like particles.
abstract: Virus-like particles containing the rotavirus (RV) internal proteins VP2 and VP6 (2/6-VLP) have been shown to induce serum and fecal antibodies as well as protection in mice after intranasal administration with a mutant of E. coli toxin, LT-R192G. To better understand the origin of fecal IgA induced by this protocol, we studied the RV-specific B cell response in systemic and mucosal lymphoid tissues using a flow cytometry assay that allows quantification and phenotypic characterization of RV-specific B lymphocytes. We also assessed the RV-specific antibody-secreting cells in the spleen and intestinal lamina propria (ILP). A remarkably high frequency of RV-specific B cells was found in the respiratory lymphoid tissues and spleen, of which only a minority expressed the alpha4beta7 integrin (intestinal homing receptor). In contrast, but in accordance with alpha4beta7 expression at the induction site, a very low response was observed in intestinal lymphoid tissues (mesenteric lymph nodes and ILP), which did not increase after a second immunization. Thus, intranasal immunization with a nonreplicating antigen does not induce an important number of RV-specific B cells with an intestinal homing profile.
authors.affiliation: Microbiologie Médicale et Moléculaire, EA562, UFRs Médecine et Pharmacie, Laboratoire de Virologie CHU, Dijon, France.
authors.forename: Agathe
authors.initials: A
authors.lastname: Ogier
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Franco
authors.forename: Annie
authors.initials: A
authors.lastname: Charpilienne
authors.forename: Jean
authors.initials: J
authors.lastname: Cohen
authors.forename: Pierre
authors.initials: P
authors.lastname: Pothier
authors.forename: Evelyne
authors.initials: E
authors.lastname: Kohli
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000906:Antibodies / Q000097:blood; D000956:Antigens, Viral / Q000008:administration & dosage* / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000276:immunology; D005243:Feces / Q000737:chemistry; D007072:Immunoglobulin D / Q000096:biosynthesis; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000276:immunology / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009297:Nasal Mucosa / Q000276:immunology / Q000821:virology; D012401:Rotavirus / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000821:virology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014771:Virion / Q000276:immunology*
chemical_list: D000906:Antibodies; D000956:Antigens, Viral; D001427:Bacterial Toxins; D036022:Capsid Proteins; D007072:Immunoglobulin D; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus; D022223:Vaccines, Subunit; D014764:Viral Proteins; D014765:Viral Vaccines