title: Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples.
abstract: We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
authors.affiliation: Dermatological, Infectious and Parasitical Diseases Department, Faculty of Medicine, Center for Microorganism Investigation, São José do Rio Preto, SP, Brazil.
authors.forename: Fabiana Rangel
authors.initials: FR
authors.lastname: Marques
authors.forename: Luciana Ventura
authors.initials: LV
authors.lastname: Cardoso
authors.forename: Carlos Eugênio
authors.initials: CE
authors.lastname: Cavasini
authors.forename: Magali Carmem de
authors.initials: MC
authors.lastname: Almeida
authors.forename: Nair Aparecida
authors.initials: NA
authors.lastname: Bassi
authors.forename: Margarete Teresa Gottardo de
authors.initials: MT
authors.lastname: Almeida
authors.forename: Andréa Regina Baptista
authors.initials: AR
authors.lastname: Rossit
authors.forename: Ricardo Luiz Dantas
authors.initials: RL
authors.lastname: Machado
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000469:parasitology*; D000818:Animals; D016022:Case-Control Studies; D003457:Cryptosporidiosis / Q000175:diagnosis*; D003458:Cryptosporidium / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity