Improved malonaldehyde assay using headspace solid-phase microextraction and its application to the measurement of the antioxidant activity of phytochemicals.
A modified malonaldehyde (MA) assay for antioxidant activity, which involves derivatization and headspace solid-phase microextraction (HS-SPME) was developed and validated. The recovery of MA as 1-methylpyrazole (product of MA and N-methylhydrazine) from a headspace of an aqueous solution containing MA, buffer, surfactant, and cod liver oil using HS-SPME with a PDMS/DVB fiber was 91.3 +/- 3.38%. MA was analyzed by a gas chromatograph with a nitrogen-phosphorus detector, and its detection limit was 0.0103 nmol/mL. The antioxidant activities of natural compounds were determined as the percentage inhibition of MA formed from cod liver oil oxidized by Fenton's reagents in the above aqueous solution. Sesamol inhibited MA formation most (86.1%), followed by eugenol (84.4%), capsaicin (80.7%), ethylvanillin (45.3%), and vanillin (31.6%) at a level of 50 microg/mL. This method did not require any organic solvents and is a simple, fast, and a highly sensitive method for MA determination.
Fujioka
Kazutoshi
K
Department of Environmental Toxicology, University of California, One Shields Avenue, Davis, California 95616, USA.
Shibamoto
Takayuki
T
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001547:Benzaldehydes / Q000494:pharmacology; D052117:Benzodioxoles; D002211:Capsaicin / Q000494:pharmacology; D002849:Chromatography, Gas / Q000379:methods*; D003060:Cod Liver Oil / Q000737:chemistry; D005054:Eugenol / Q000494:pharmacology; D008315:Malondialdehyde / Q000032:analysis*; D010084:Oxidation-Reduction; D010636:Phenols / Q000494:pharmacology; D010944:Plants / Q000737:chemistry*
D000975:Antioxidants; D001547:Benzaldehydes; D052117:Benzodioxoles; D010636:Phenols; D005054:Eugenol; D008315:Malondialdehyde; D003060:Cod Liver Oil; C025583:sesamol; C100058:vanillin; D002211:Capsaicin