Characterization of Potato virus Y (PVY) molecular determinants involved in the vein necrosis symptom induced by PVYN isolates in infected Nicotiana tabacum cv. Xanthi.
Viral molecular determinant(s) involved in the tobacco vein necrosis (TVN) symptom induced by necrotic isolates of Potato virus Y (PVY) on Nicotiana tabacum cv. Xanthi leaves remain undetermined. Reference isolates belonging to PVY(N) (infectious PVY(N)-605 clone) and PVY(O) (PVY(O)-139) were used to produce PVY chimeric genomes by using reverse-genetic techniques. These chimeric clones were inoculated biolistically onto Nicotiana clevelandii plants to establish the clone, prior to being tested on N. tabacum for their ability to induce TVN symptoms. Comparison between sequence data and symptoms observed for each mutated PVY construct shows that the C-terminal part of the multifunctional HC-Pro protein includes two residues (K(400) and E(419)) that are involved in TVN induced by PVY(N) isolates. Site-directed mutagenesis was used to confirm that these two HC-Pro residues are involved in the TVN phenotype.
Tribodet
Michel
M
INRA/ENSA, Unité Mixte de Recherche Biologie des Organismes et des Populations appliquée à la Protection des Plantes (BiO3P), Domaine de la Motte BP 35327, F-35653 Le Rheu cedex, France.
Glais
Laurent
L
FNPPPT (Fédération Nationale des Producteurs de Plants de Pomme de Terre), 9 rue d'Athènes, F-75009 Paris, France.|INRA/ENSA, Unité Mixte de Recherche Biologie des Organismes et des Populations appliquée à la Protection des Plantes (BiO3P), Domaine de la Motte BP 35327, F-35653 Le Rheu cedex, France.
Kerlan
Camille
C
INRA/ENSA, Unité Mixte de Recherche Biologie des Organismes et des Populations appliquée à la Protection des Plantes (BiO3P), Domaine de la Motte BP 35327, F-35653 Le Rheu cedex, France.
Jacquot
Emmanuel
E
INRA/ENSA, Unité Mixte de Recherche Biologie des Organismes et des Populations appliquée à la Protection des Plantes (BiO3P), Domaine de la Motte BP 35327, F-35653 Le Rheu cedex, France.
D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus