A mammalian dpy-19 homologue is expressed in GABAergic neurons.
Gsh2 homeobox transcription factors play a crucial role in the development of GABAergic neurons. Caenorhabditis elegans's mab-5 gene is homologous to Gsh2; its expression is controlled by dpy-19. This study produced the polyclonal anti-mammalian DPY-19 (MDPY-19) antibody and showed the distribution of anti-MDPY-19 immunopositive cells. In addition, the mammalian dpy-19 (Mdpy-19) 5'-flanking region was analyzed by in vivo transient transfection assays. Mdpy-19 is expressed in ependymal cells in the adult rat brain, embryonic neuroepithelial cells, and cultured neural stem cells. In the adult rat ventricular zone, immunoreactivity with MDPY-19 of the dorsal area is stronger than that of the ventral area. Embryonic neuroepithelial cells and radial glial cells show strong anti-MDPY-19 immunoreactivity. We created the Mdpy-19 green fluorescent protein (GFP) reporter gene. Our results show that Mdpy-19 is expressed in neural stem cells and progenitor cells, especially radial glial cells. Only ependymal cells among differentiated cells express Mdpy-19 gene. However, the possibility exists that the Mdpy-19 gene is able to transcript in GABAergic neurons. It is suggested that a factor existing in mature neurons withdraws the expression of Mdpy-19.
Kurokawa
Kiyoshi
K
Department of Anatomy, Shiga University of Medical Science, Seta-Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan. kurokawa@belle.shiga-med.ac.jp
Tamura
Yasuhisa
Y
Kataoka
Yosky
Y
Yamada
Hisao
H
Nakamura
Takaaki
T
Taki
Kosuke
K
Kudo
Motoi
M
D000818:Animals; D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins / Q000032:analysis; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D064231:Nestin; D009474:Neurons / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; C417031:DPY-19 protein, C elegans; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D005680:gamma-Aminobutyric Acid