[Extraction and purification of psoralen from Psoralea corylifolia L].
OBJECTIVE
To establish a method for extracting psoralen from the seed of Psoralea corylifolia L.


METHODS
Crushed seed of Psoralea corylifolia L. was soaked with 50% ethyl alcohol solution, and the leaching solution was filtrated and distilled to remove alcohol. A slurry of the sediment was obtained and dissolved in methyl alcohol (MeOH), followed by treatment with active carbon for decoloration and evaporation of MeOH, resulting in a lamellar crystal after the sediment was kept still overnight. MeOH was applied to dilute the sticky solution, and after filteration, a yellow crystal was obtained, recrystallization of which in MeOH yielded white needle-like crystals. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were employed to determine the purity of the resultant substance, and UV and H1-NMR applied to identify its chemical structure.


CONCLUSIONS
The resultant crystal is confirmed to be pure monomers of psoraren with a yield of 0.147% from the seed (m/m) by this simple extraction method.
Liu
Zhi-lin
ZL
Outpatient Clinic, Guangdong University of Foreign Studies, Guangzhou 510420, China.
Zhang
Xiang-nian
XN
Zhao
Shu-jin
SJ
Zhou
Ri-xiao
RX
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005363:Ficusin / Q000737:chemistry / Q000302:isolation & purification*; D029907:Psoralea / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005363:Ficusin