Optical and MRI multifunctional nanoprobe for targeting gliomas.
A multifunctional nanoprobe capable of targeting glioma cells, detectable by both magnetic resonance imaging and fluorescence microscopy, was developed. The nanoprobe was synthesized by coating iron oxide nanoparticles with covalently bound bifunctional poly(ethylene glycol) (PEG) polymer, which were subsequently functionalized with chlorotoxin and the near-infrared fluorescing molecule Cy5.5. Both MR imaging and fluorescence microscopy showed significant preferential uptake of the nanoparticle conjugates by glioma cells. Such a nanoprobe could potentially be used to image resections of glioma brain tumors in real time and to correlate preoperative diagnostic images with intraoperative pathology at cellular-level resolution.
Veiseh
Omid
O
Department of Materials Science & Engineering, University of Washington, Seattle, Washington 98195, USA.
Sun
Conroy
C
Gunn
Jonathan
J
Kohler
Nathan
N
Gabikian
Patrik
P
Lee
Donghoon
D
Bhattarai
Narayan
N
Ellenbogen
Richard
R
Sze
Raymond
R
Hallahan
Andrew
A
Olson
Jim
J
Zhang
Miqin
M
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry*; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D032383:Myocytes, Cardiac / Q000166:cytology; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D019047:Phantoms, Imaging; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D012604:Scorpion Venoms / Q000737:chemistry
C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005290:Ferric Compounds; D012604:Scorpion Venoms; C091539:Chlorotoxin; C000499:ferric oxide; D011092:Polyethylene Glycols