title: RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1.
abstract: Rho GTPases are crucial regulators of the actin cytoskeleton and they play a role in the control of membrane trafficking. In contrast to the close family members RhoA and RhoC, RhoB localises to endosomes and delays epidermal growth factor receptor traffic. Here, we show that activated RhoB induces the peripheral distribution of endosomes, which align along subcortical actin stress fibres and are surrounded by an actin coat. The Diaphanous-related formin, Dia1, is recruited to endosomes by activated RhoB. Dia1 is required for the formation of the actin coat around endosomes downstream of RhoB, connecting membrane trafficking with the regulation of actin dynamics.
authors.affiliation: The Netherlands Cancer Institute, Division of Tumour Biology, Amsterdam.
authors.forename: Mar
authors.initials: M
authors.lastname: Fernandez-Borja
authors.forename: Lennert
authors.initials: L
authors.lastname: Janssen
authors.forename: Desiree
authors.initials: D
authors.lastname: Verwoerd
authors.forename: Peter
authors.initials: P
authors.lastname: Hordijk
authors.forename: Jacques
authors.initials: J
authors.lastname: Neefjes
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D048430:Cell Shape; D003599:Cytoskeleton / Q000378:metabolism; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D000080865:Formins; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D015398:Signal Transduction; D020743:rhoB GTP-Binding Protein / Q000378:metabolism*
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C108962:DIAPH1 protein, human; D000080865:Formins; D020743:rhoB GTP-Binding Protein