[Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection].
OBJECTIVE
To assess the value of dynamic monitoring of soluble human lymphocytic antigen-I (sHLA-I) in the prediction of graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (PBSCT).


METHODS
Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantitatively detect serum sHLA-I. The serum samples for testing were added into W6 32 monoclonal antibody-coated microtiter plate and incubated with anti-beta2m HRP followed by color development with the addition of the substrate. Serum sHLA-I level was measured in 63 healthy blood donors of Shanghai and in 24 PBSCT recipients before and and at different time points after the operation.


RESULTS
No changes in sHLA-I levels occurred in allogeneic PBSCT recipients without GVHD or with only grade I GVHD, but sHLA-I reached high levels in patients suffering GVHD II-IV(P<0.05), which was effectively lowered by the application of immunosuppressants.


CONCLUSIONS
Measurements of sHLA-I levels can be valuable for monitoring GVHD after PBSCT.
Xia
Rong
R
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Qiu
Hui-ying
HY
Zhang
Wei-ping
WP
Cao
Qiong
Q
Lan
Jiong-cai
JC
Wen
Hong-sheng
HS
Wang
Jian-min
JM
D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000175:diagnosis*; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*
D015395:Histocompatibility Antigens Class I