title: In vitro interaction between surface-treated Ti-6Al-4V titanium alloy and human peripheral blood mononuclear cells.
abstract: The Ti-6Al-4V titanium alloy is widely employed as an implant material. The effects of Ti-6Al-4V samples, tested in both an untreated state and one in which the samples were subjected to a glow-discharge treatment performed with the use of air, on human peripheral blood mononuclear cells (PBMC) were studied. Apoptosis, undetectable after 24-h contact of PBMC with the two sample types, is induced after 48 h by treated samples, and, after 48 h, but in the presence of 1.5 microg/mL PHA, by both sample types. The expression of intercellular adhesion molecule-1 (ICAM-1) always increases, in comparison with control, in PBMC put in contact with the two sample types. In the same way, a remarkable increase in tumor necrosis-alpha (TNF-alpha) release in the culture medium is registered, when PBMC are put in contact with the two sample types for 24 and 48 h. Human umbilical-vein endothelial cells (HUVEC) cocultured for 48 h with PBMC, previously incubated with the two sample types for 24 h, show an increase in ICAM-1 and vascular cell adhesion molecule-1 (VCAM-1) protein expression in comparison with control (HUVEC cocultured with control PBMC), indicating that inflammatory phenomena might occur. Taken together, these results suggest that, although plasma-treated titanium alloy shows a better biocompatibility in comparison with the untreated one, attention must be paid to the careful control of the first signs of inflammation.
authors.affiliation: Department of Biochemical Sciences of the University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Martinesi
authors.forename: Sara
authors.initials: S
authors.lastname: Bruni
authors.forename: Maria
authors.initials: M
authors.lastname: Stio
authors.forename: Cristina
authors.initials: C
authors.lastname: Treves
authors.forename: Francesca
authors.initials: F
authors.lastname: Borgioli
mesh_terms: D000497:Alloys; D017209:Apoptosis; D015153:Blotting, Western; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000497:Alloys; D003470:Culture Media; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; C031462:titanium alloy (TiAl6V4); D014025:Titanium