Assessment of the integrity of human oocytes retrieved from cryopreserved ovarian tissue after xenotransplantation.
BACKGROUND
Previous studies showed that immature oocytes stored in ovarian tissue could develop to the mature stage after transplantation. However, the quality and competency of the oocytes developed in xenografted ovarian tissue have never been investigated. As a pilot study to investigate this uncharted issue, we evaluated microtubule organization and chromatin configuration of human oocytes harvested from xenografted frozen-thawed ovarian tissue.


METHODS
Frozen-thawed human ovarian tissue was transplanted into severe combined immunodeficient mice. All animals were stimulated with gonadotrophin from 20 weeks after transplantation. Grafts were recovered 36 h after hCG administration. The oocytes were retrieved from the antral follicles (>2 mm diameter), cultured in vitro, stained for microtubule and chromatin localization.


RESULTS
Five oocytes from 21 female mice and seven oocytes from nine male mice were retrieved. Immunocytochemical examinations of these oocytes after in vitro maturation revealed only two developed to the metaphase II stage. Most oocytes were between prophase and metaphase with abnormal microtubule organization and chromatin configuration.


CONCLUSIONS
Immature oocytes in stored human ovarian tissue can grow to maturity in host animals after xenotransplantation. Retrieval of oocytes from the xenograft can be carried out and is reproducible. However, many oocytes, grown in host animals and further matured in vitro, showed aberrant microtubule organization and chromatin patterns.
Kim
S Samuel
SS
Department of OB/GYN, Eulji University School of Medicine, Seoul, Korea. medssk@attglobal.net
Kang
Hee Gyu
HG
Kim
Nam Hyung
NH
Lee
Hoi Chang
HC
Lee
Hyang Heun
HH
D000328:Adult; D000818:Animals; D002843:Chromatin; D015925:Cryopreservation; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008870:Microtubules; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development / Q000637:transplantation*; D006080:Ovarian Follicle / Q000166:cytology; D010865:Pilot Projects; D014015:Tissue Banks; D014183:Transplantation, Heterologous
D002843:Chromatin