Physicochemical characterization of poly(L-lactic acid) and poly(D,L-lactide-co-glycolide) nanoparticles with polyethylenimine as gene delivery carrier.
Polymer nanoparticles have been used as non-viral gene delivery systems and drug delivery systems. In this study, biodegradable poly(L-lactic acid) (PLA)/polyethylenimine (PEI) and poly(D,L-lactide-co-glycolide) (PLGA)/PEI nanoparticles were prepared and characterized as gene delivery systems. The PLA/PEI and PLGA/PEI nanoparticles, which were prepared by a diafiltration method, had spherical shapes and smooth surface characteristics. The size of nanoparticles was controlled by the amount of PEI, which acted as a hydrophilic moiety, which effectively reduced the interfacial energy between the particle surface and the aqueous media. The nanoparticles showed an excellent dispersive stability under storage in a phosphate-buffered saline solution for 12 days. The positive zeta-potentials for the nanoparticles decreased and changed to negative values with increasing plasmid DNA (pDNA) content. Agarose gel electrophoresis showed that the complex formation between the nanoparticles and the pDNA coincided with the zeta-potential results. The results of in vitro transfection and cell viability on HEK 293 cells indicated that the nanoparticles could be used as gene delivery carriers.
Kim
In-Sook
IS
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Yongbong-dong, Bug-gu, Gwangju 500-757, Republic of Korea.
Lee
Soo-Kyung
SK
Park
Yu-Mi
YM
Lee
Yong-Bok
YB
Shin
Sang-Chul
SC
Lee
Kang Choon
KC
Oh
In-Joon
IJ
D002470:Cell Survival; D016503:Drug Delivery Systems; D004355:Drug Stability; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D049329:Nanostructures; D010316:Particle Size; D011091:Polyesters; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection
D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D011094:Polyethyleneimine