Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China.
OBJECTIVE
To investigate the association between single nucleotide polymorphism (SNP) in promoter of the DNA methyltransferase 3B (DNMT3B) gene and risk for development and lymphatic metastasis of gastric cardiac adenocarcinoma (GCA).


METHODS
The hospital based case-control study included 212 GCA patients and 294 control subjects without overt cancer. The DNMT3B SNP was genotyped by PCR and restriction fragment length polymorphism (RFLP) analysis.


RESULTS
The C/C genotype was not detected in both GCA patients and controls. In control subjects, the frequency of T/T and C/T genotypes was 94.9% and 5.1% respectively, and that of T and C alleles was 97.4% and 2.6%, respectively. The genotype and allelotype distribution in the GCA patients was not significantly different from that in controls (P=0.34 and 0.33, respectively). When stratified by smoking status and family history of upper gastrointestinal cancer, significant difference in the genotype distribution was not observed between GCA patients and controls. The distribution of DNMT3B genotypes in GCA patients with or without lymphatic metastasis did not show significant difference (P=0.42).


CONCLUSIONS
The distribution of DNMT3B SNP in North China is distinct from that in Caucasians. Although this SNP has been associated with susceptibility to lung, head, neck and breast cancer, it may not be used as a stratification marker to predict susceptibility and lymphatic metastasis of GCA, at least in the population of North China.
Wang
Yi-Min
YM
Hebei Cancer Institute, Hebei Medical University, Jiankanglu 12, Shijiazhuang 050011, Hebei Province, China.
Wang
Rui
R
Wen
Deng-Gui
DG
Li
Yan
Y
Guo
Wei
W
Wang
Na
N
Wei
Li-Zhen
LZ
He
Yu-Tong
YT
Chen
Zhi-Feng
ZF
Zhang
Xiu-Feng
XF
Zhang
Jian-Hui
JH
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D006338:Heart Neoplasms / Q000201:enzymology / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics*; D044465:White People / Q000235:genetics; D000097584:DNA Methyltransferase 3B
D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B