Preliminary characterisation of Toxoplasma gondii isolates from Zimbabwe, with stage-specific monoclonal antibodies.
Cell-culture-derived clones of eight Toxoplasma gondii isolates from Zimbabwe were characterised in IFAT with a panel of five monoclonal antibodies (mAb). Each clone had been established from a single murine brain cyst. The antibodies were bradyzoite-specific (4.3), tachyzoite-specific (4.25, 5.1 and anti-P(30)), or tachyzoite- and bradyzoite-specific (5.15). Their strong reactivity with the bradyzoite-specific mAb 4.3 and their weaker reactivity with the tachyzoite-specific 4.25, 5.1 and anti-P(30) indicated that all the isolates are probably of genetic type II. Each of the isolates reacted in the IFAT in a similar way to the Danish reference strain of T. gondii, SSI-119.
Hove
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Parasitology Section, Faculty of Veterinary Science, University of Zimbabwe, Mount Pleasant, Harare. thove@vet.uz.ac.zw
Lind
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Mukaratirwa
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D001921:Brain / Q000469:parasitology; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006041:Goats / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep / Q000469:parasitology; D013552:Swine / Q000469:parasitology; D014122:Toxoplasma / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan