title: Entamoeba histolytica: comparative genomics of the pre-mRNA 3' end processing machinery.
abstract: We report here the pre-mRNA 3' end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3' end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identified 16 putative genes encoding for cleavage/polyadenylation factors in this parasite. E. histolytica pre-mRNA 3' end processing machinery does not seem to contain homologous genes coding for human Symplekin, CFIm59, and CFIm68 proteins, neither sequences related to yeast Pta1p and Hrp1p. Protein sequence comparisons among E. histolytica, yeast, and human showed little variation in their functional domains through evolutive scale. E. histolytica pre-mRNA 3' end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. From these analyses, we propose a hypothetical working model for the pre-mRNA 3' end processing machinery in E. histolytica.
authors.affiliation: Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo # 290, Col. del Valle, CP 03110, Mexico D.F., Mexico. genomicas@yahoo.com.mx
authors.forename: César
authors.initials: C
authors.lastname: López-Camarillo
authors.forename: Esther
authors.initials: E
authors.lastname: Orozco
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Marchat
mesh_terms: D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D039223:Cleavage And Polyadenylation Specificity Factor / Q000737:chemistry / Q000235:genetics; D039241:Cleavage Stimulation Factor / Q000737:chemistry / Q000235:genetics; D016384:Consensus Sequence; D017124:Conserved Sequence; D004748:Entamoeba histolytica / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D023281:Genomics; D006801:Humans; D008969:Molecular Sequence Data; D026723:Polyadenylation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; D039223:Cleavage And Polyadenylation Specificity Factor; D039241:Cleavage Stimulation Factor; D015800:Protozoan Proteins; D012333:RNA, Messenger