Neuroanatomical, immunocytochemical, and physiological studies of the pharyngeal retractor muscle and its putative regulatory neurons playing a role in withdrawal and feeding in the snail, Helix pomatia.
We describe the neurons regulating two separate functions of the pharyngeal retractor muscle (PRM), namely sustained contraction during body withdrawal and rhythmic phasic contractions during feeding, in the snail, Helix pomatia. The distribution of central neurons innervating the PRM is organized into two main units; one in the buccal-cerebral ganglion complex, the other in the subesophageal ganglion complex. Serotonin- (5-HT-), FMRFamide- (FMRFa-), and tyrosine-hydroxylase-immunostained neurons are present among the PRM neurons that densely innervate the PRM. 5HT both decreases and increases the amplitude of the electrically evoked contraction between concentrations of 0.1 microM and 1 microM. Dopamine (DA) only decreases the amplitude of contraction at a 1-microM threshold concentration. In contrast, FMRFa increases the amplitude of the contraction and slightly elevates the tone of the PRM but requires a higher threshold (10 microM). Assay by high-performance liquid chromatography of 5HT and DA in the PRM has shown that the 5HT level decreases during locomotion but increases during feeding, whereas the DA level increases during locomotion but slightly decreases during feeding. Thus, different segments of the PRM are innervated by neurons from different loci within the central nervous system. The segments of the PRM distal to the pharynx are innervated from loci of the subesophageal ganglion complex suggesting that they mediate withdrawal. The proximal segment of the PRM is innervated from cerebral and buccal loci indicating that these neurons mediate the feeding rhythm produced by buccal and cerebral feeding central pattern generators to induce rhythmic phasic contractions in the PRM during feeding.
Hernádi
László
L
Department of Experimental Zoology, Balaton Limnological Research Institute, Hungarian Academy of Sciences, Tihany. hernadi@tres.blki.hu
Vehovszky
Agnes
A
Hiripi
László
L
Györi
János
J
Walker
Robert J
RJ
Elekes
Károly
K
D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D019835:FMRFamide / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006372:Helix, Snails / Q000166:cytology* / Q000502:physiology; D007150:Immunohistochemistry; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009068:Movement / Q000187:drug effects / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009132:Muscles / Q000166:cytology / Q000294:innervation* / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010614:Pharynx / Q000166:cytology / Q000294:innervation* / Q000502:physiology; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D002395:Catecholamines; D012701:Serotonin; D019835:FMRFamide; D004298:Dopamine