title: Inhibitory effect of glycolipids from spinach on in vitro and ex vivo angiogenesis.
abstract: Anti-cancer activity of some glycolipids from animals and plants has been demonstrated, although it was unknown whether the glycolipids had anti-angiogenic activity. The effects of the purified three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), and sulfoquinovosyl diacylglycerol (SQDG) from the green vegetable spinach (Spinacia oleracea L.) were examined on in vitro and ex vivo angiogenesis models. MGDG and SQDG suppressed microvessel growth in an ex vivo angiogenesis model using a rat aortic ring. The glycolipids inhibited human umbilical vein endothelial cell (HVUEC) tube formation on a reconstituted basement membrane and HUVEC proliferation. These results demonstrate that glycolipids from spinach would suppress tumor growth by suppressing angiogenesis and might be candidates for anti-cancer or anti-angiogenic materials.
authors.affiliation: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan. kmatsuba@fhw.oka-pu.ac.jp
authors.forename: Kiminori
authors.initials: K
authors.lastname: Matsubara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mizushina
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mori
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Nakajima
authors.forename: Michiko
authors.initials: M
authors.lastname: Fuchigami
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Takahiko
authors.initials: T
authors.lastname: Hada
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000254:growth & development; D001808:Blood Vessels / Q000187:drug effects* / Q000254:growth & development; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D038983:Galactolipids / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D018724:Spinacia oleracea / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D020533:Angiogenesis Inhibitors; D038983:Galactolipids; D006017:Glycolipids; D010936:Plant Extracts; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; C013694:sulfoquinovosyl diglyceride