[Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant].
OBJECTIVE
To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant.


METHODS
Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores.


RESULTS
The rate of germination effects were 68.0% under 6 mmol/L inosine at 37 degrees C, pH 7.9; 74.5% under 70 mmol/L L-alanine at 30 degrees C, pH 8.9; and 85.6% under 6 mmol/L inosine and 70 mmol/L L-alanine at 37 degrees C, pH 8.2. Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed.


CONCLUSIONS
Under suitable environment, the nutrient germinant with inosine and L-alanine might be helpful for germinating the bacillus anthracoides spores.
Zhang
Li-jun
LJ
Department of Military Epidemiology, Academy of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China.
Xiong
Hong-yan
HY
Zhang
Yao
Y
Ma
Fei
F
Li
Li
L
D000409:Alanine / Q000494:pharmacology*; D001408:Bacillus anthracis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media; D007288:Inosine / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
D001426:Bacterial Proteins; D003470:Culture Media; D007288:Inosine; D000409:Alanine