title: Genes transactivated by hepatitis C virus core protein, a microarray assay.
abstract: OBJECTIVE
  To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.
  
  
  METHODS
  Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.
  
  
  RESULTS
  The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method.
  
  
  CONCLUSIONS
  HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.
authors.affiliation: Department of Infectious Diseases, The First Affilated, Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. liumin3262@sohu.com
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Shao
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Lin
mesh_terms: D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D015967:Gene Expression Regulation, Viral; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000503:physiopathology*; D006801:Humans; D008113:Liver Neoplasms; D020411:Oligonucleotide Array Sequence Analysis