[The expression of growth hormone release factor gene carried with PLGA microspheres in muscle tissue and its effect on mouse growth].
Injectable sustained-release pcDNA3-GRF (1-32) microspheres were prepared by double emulsion-in liquid evaporation process,using biodegrable poly lactic-co-glycolic acid as carrier. The enrapment efficiency, mean particle size, drug content thus prepared were 69%, 2.20 microm, 8% and 70% respectively. The result of transfection in vivo showed that after 30 days, accumulative increased body weights on the group injected with pcDNA3-GRF (1-32) microspheres was significantly higher than those group injected with naked plasmid (12.87%), plasmid-empty microspheres (19.72%) and saline (58.58%) respectively. PCR and RT-PCR showed that the expression level of GRF gene on the group injected with pcDNA3-GRF (1-32) microspheres was the highest. GRF gene released by microspheres was still detected after 30 days. In conclusion, pcDNA3-GRF (1-32) microspheres have a controlled release effect and GRF gene could be successfully transfected into muscle cells of mouse by microspheres with higher efficacy and stronger biological function.
Ren
Xiao-Hui
XH
Department of Biochemistry, Changchun University of Agricultural and Animal Sciences, Changchun 130062, China.
Zhang
Yong-Liang
YL
Liu
Song-Cai
SC
Dai
Jian-Wei
JW
D000818:Animals; D001835:Body Weight; D004247:DNA / Q000008:administration & dosage*; D006128:Growth; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics*; D019344:Lactic Acid / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008863:Microspheres; D018482:Muscle, Skeletal / Q000378:metabolism*; D010957:Plasmids; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016133:Polymerase Chain Reaction
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA; D013007:Growth Hormone-Releasing Hormone