[Expression and clinical significance of KISS-1 and GPR54 mRNA in endometrial carcinoma].
OBJECTIVE
To investigate the expression and clinical significance of KISS-1 mRNA and GPR54 mRNA in endometrial carcinoma.


METHODS
The expression of KISS-1 mRNA and GPR54 mRNA in 32 patients with endometrial carcinoma, 10 patients with endometrial intraepithelial neoplasia (EIN) and 12 patients with normal endometrium was detected by reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
The positive rate of KISS-1 mRNA in endometrial carcinoma, EIN and normal endometrium was 37.5%, 80.0% and 83.3% respectively (endometrial carcinoma vs EIN or normal endometrium, P < 0.05). The expression of KISS-1mRNA in patients with endometrial carcinoma was correlated with its clinical stage, myometrial invasion and lymph node metastasis (P < 0.05). In endometrial carcinoma, the more advanced clinical stage, the lower expression of KISS-1 mRNA was detected. The positive rate of GPR54 mRNA in endometrial carcinoma, EIN and normal endometrium was 78.1%, 70.0% and 66.7% respectively, with no significant statistical difference (P > 0.05). It was not correlated with the clinical stage, histology grade, myometrial invasion or lymph node metastasis (P > 0.05).


CONCLUSIONS
The interaction of KISS-1 and GPR54 may play an important role in inhibiting the invasion and metastasis of endometrial carcinoma.
Jiang
Tao
T
Department of Gynecology and Obstetrics, Second Hospital, China Medical University, Shenyang 110004, China.
Zhang
Shu-lan
SL
Lin
Bei
B
Meng
Li-rong
LR
Gao
Hong
H
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D059648:Kisspeptins; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D000074502:Receptors, Kisspeptin-1; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
C106628:KISS1 protein, human; C118688:KISS1R protein, human; D059648:Kisspeptins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1; D025521:Tumor Suppressor Proteins