title: Detection of 1,2-hydride shifts in the formation of euph-7-ene by the squalene-tetrahymanol cyclase of Tetrahymena pyriformis.
abstract: Incubation of samples of 2,3-dihydrosqualene, specifically labeled with deuterium at either carbon position 7 or 11, with an enzyme extract from Tetrahymena pyriformis, containing a squalene-tetrahymanol cyclase, provided specimens of euph-7-enes displaying deuterium patterns consistent with the biosynthetic operation of two consecutive 1,2-hydride shifts.
authors.affiliation: Laboratorium für Organische Chemie, ETH-Hönggerberg HCI H307, CH-8093, Zürich, Switzerland.
authors.forename: José-Luis
authors.initials: JL
authors.lastname: Giner
authors.forename: Stefano
authors.initials: S
authors.lastname: Rocchetti
authors.forename: Serge
authors.initials: S
authors.lastname: Neunlist
authors.forename: Michel
authors.initials: M
authors.lastname: Rohmer
authors.forename: Duilio
authors.initials: D
authors.lastname: Arigoni
mesh_terms: D000818:Animals; D003903:Deuterium; D008190:Lyases / Q000378:metabolism*; D013185:Squalene / Q000031:analogs & derivatives* / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000201:enzymology*; D014315:Triterpenes / Q000378:metabolism
chemical_list: D014315:Triterpenes; C517934:euph-7-ene; D013185:Squalene; D003903:Deuterium; D008190:Lyases; C022651:squalene cyclase