title: Incomplete replacement of docosahexaenoic acid by n-6 docosapentaenoic acid in the rat retina after an n-3 fatty acid deficient diet.
abstract: When sources of n-3 fatty acids are not present in the diet, nervous system docosahexaenoic acid (22:6n3) is replaced by docosapentaenoic acid (22:5n6). Dams were fed either an n-3 deficient diet or one containing alpha-linolenic acid (18:3n3) and 22:6n3 throughout pregnancy and lactation. Their male offspring at weaning also received either the n-3 deficient or n-3 adequate diets and were sacrificed at 5, 10, 20, 50 and 91 days of age. Retinal lipids were extracted and analysed by gas chromatography for fatty acyl content. The percentage of retinal 22:6n3 increased continuously over the 13 week course of the experiment but reached its maximal concentration around day 20. Non-reciprocal replacement of 22:6n3 by 22:5n6 was observed at postnatal day 20 and 50 but not at other time points. Complete replacement of 22:6n3 was apparent if elevations in both 22:5n6 and docosatetraenoic acid (22:4n6) were considered. These data indicate that during the rapid period of accretion of retinal 22:6n3 around postnatal day 20, the supply of 22:5n6 to the retina was inadequate to completely replace 22:6n3 in n-3 deficient rats.
authors.affiliation: Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Room 3N-07, MSC 9410, Bethesda, MD 20892-9410, USA. nsalem@niaaa.nih.gov
authors.forename: Norman
authors.initials: N
authors.lastname: Salem
authors.forename: James
authors.initials: J
authors.lastname: Loewke
authors.forename: Janice N
authors.initials: JN
authors.lastname: Catalan
authors.forename: Sharon
authors.initials: S
authors.lastname: Majchrzak
authors.forename: Toru
authors.initials: T
authors.lastname: Moriguchi
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D004032:Diet; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D019787:Linoleic Acid / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000254:growth & development / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid; C026219:docosapentaenoic acid