Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays.
Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-beta1 and LOXL2) and iron metabolism (TFR and FTL).
Pascal
Thierry
T
Unit of Research on Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61 B-5000 Namur, Belgium.
Debacq-Chainiaux
Florence
F
Chrétien
Aline
A
Bastin
Coralie
C
Dabée
Anne-France
AF
Bertholet
Vincent
V
Remacle
José
J
Toussaint
Olivier
O
D016922:Cellular Senescence / Q000235:genetics*; D000431:Ethanol / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007501:Iron / Q000378:metabolism; D008168:Lung / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000431:Ethanol; D020122:tert-Butylhydroperoxide; D007501:Iron