title: A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge.
abstract: We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal anti-enterovirus strategy.
authors.affiliation: Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jeonghyun
authors.initials: J
authors.lastname: Ahn
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Jun
authors.forename: Hui Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Yoon
authors.forename: DongHou
authors.initials: D
authors.lastname: Kim
authors.forename: Chul-Hyun
authors.initials: CH
authors.lastname: Joo
authors.forename: Yoo Kyum
authors.initials: YK
authors.lastname: Kim
authors.forename: Heuiran
authors.initials: H
authors.lastname: Lee
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D004770:Enterovirus / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000998:Antiviral Agents; D034741:RNA, Small Interfering; D014764:Viral Proteins; C097017:coxsackie B3 virus protein 2B