title: Direct measurement of gastric H+/K+-ATPase activities in patients with or without Helicobacter pylori-associated chronic gastritis.
abstract: OBJECTIVE
  The role of Helicobacter pylori (H pylori) infection in gastric acid secretion of patients with chronic gastritis remains controversial. This study was designed to elucidate the effect of H pylori on H+/K+-ATPase activities in gastric biopsy specimens.
  
  
  METHODS
  Eighty-two patients with chronic gastritis who had undergone upper endoscopy were included in this study. H pylori infection was confirmed by rapid urease test and histology. Gastric H+/K+-ATPase activities and serum gastrin concentrations were measured by an enzymatic method and radioimmunoassay, respectively. For those patients who received triple therapy for eradicating H pylori, changes in the activity of gastric H+/K+-ATPase and serum gastrin levels were also measured.
  
  
  RESULTS
  The mean gastric H+/K+-ATPase activity in H pylori-positive group (42 patients) was slightly higher than that in H pylori-negative group (29 patients) (169.65+/-52.9 and 161.38+/-43.85 nmol Pi/(mg.h), respectively, P=0.301). After eradication of H pylori, the gastric H+/K+-ATPase activities slightly decreased compared to prior therapy (165.03+/-59.50 and 158.42+/-38.93 nmol Pi/(mg.h), respectively, P=0.805). The mean basal gastrin concentration was slightly higher in H pylori-positive patients than in H pylori-negative patients (87.92+/-39.65 pg/mL vs 75.04+/-42.57 pg/mL, P=0.228). The gastrin levels fell significantly after the eradication of H pylori. (Before treatment 87.00+/-30.78 pg/mL, after treatment 64.73+/-18.96 pg/mL, P=0.015).
  
  
  CONCLUSIONS
  Gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronic gastritis.
authors.affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University Hospital, Bangkok 10330, Thailand. d.thong@mailcity.com
authors.forename: Duangporn
authors.initials: D
authors.lastname: Thong-Ngam
authors.forename: Pisit
authors.initials: P
authors.lastname: Tangkijvanich
authors.forename: Pichet
authors.initials: P
authors.lastname: Sampatanukul
authors.forename: Paungpayom
authors.initials: P
authors.lastname: Prichakas
authors.forename: Varocha
authors.initials: V
authors.lastname: Mahachai
authors.forename: Piyaratana
authors.initials: P
authors.lastname: Tosukowong
mesh_terms: D002908:Chronic Disease; D005753:Gastric Mucosa / Q000201:enzymology*; D005755:Gastrins / Q000097:blood; D005756:Gastritis / Q000201:enzymology / Q000382:microbiology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000201:enzymology*; D016480:Helicobacter pylori; D006801:Humans; D012016:Reference Values
chemical_list: D005755:Gastrins; D017506:H(+)-K(+)-Exchanging ATPase