Mass spectrometry-based quantitative proteomics.
A major aim of present-day proteomics is to study changes in protein expression levels at a global level, ideally monitoring all proteins present in cells or tissue. Mass spectrometry is a well-respected technology in proteomics that is widely used for the identification of proteins. More recently, methodologies have been introduced showing that mass spectrometry can also be used for protein quantification. This article reviews various mass spectrometry-based technologies in quantitative proteomics, highlighting several interesting applications in areas ranging from cell biology to clinical applications.
Heck
Albert J R
AJ
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. a.j.r.heck@chem.uu.nl
Krijgsveld
Jeroen
J
D000818:Animals; D002458:Cell Fractionation; D015870:Gene Expression; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*
D020543:Proteome