Polymorphisms of B7 (CD80 and CD86) genes do not affect disease susceptibility to sarcoidosis.
BACKGROUND
B7 proteins (CD80, CD86) are costimulatory molecules expressed on antigen-presenting cells and are essential factors for T cell activation.


OBJECTIVE
The aim of this study is to investigate the relationship of B7 gene polymorphisms either to disease susceptibility or to cell profile of bronchoalveolar lavage (BAL) fluid in Japanese sarcoidosis patients.


METHODS
Gene polymorphisms located in CD80 promoter, CD80 exon 3 and exon 8 of CD86 were examined in 146 Japanese sarcoidosis patients and 157 healthy controls using single-strand conformation polymorphism and direct sequencing. The distribution of genotypes was compared between the two groups. BAL fluid cell profiles were compared for the various genotypes of the different polymorphisms for the 62 patients who underwent BAL.


RESULTS
There were no significant differences in the distribution of genotypes or allele frequencies for all polymorphisms between sarcoidosis and controls. There were no significant differences in BAL fluid cell profiles among the different genotype groups of the various polymorphisms studied.


CONCLUSIONS
There was no relationship between the B7 gene polymorphisms studied and disease susceptibility or BAL fluid cell profiles in Japanese sarcoidosis patients.
Handa
Tomohiro
T
Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Shogoin Kawaracho, Kyoto, Japan.
Nagai
Sonoko
S
Ito
Isao
I
Tabuena
Rollin
R
Shigematsu
Michio
M
Hamada
Kunio
K
Kitaichi
Masanori
M
Izumi
Takateru
T
Aoyama
Tomoki
T
Toguchida
Junya
J
Mishima
Michiaki
M
D015703:Antigens, CD / Q000235:genetics*; D018122:B7-1 Antigen / Q000235:genetics*; D051940:B7-2 Antigen; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic / Q000235:genetics*; D012507:Sarcoidosis / Q000235:genetics* / Q000276:immunology
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins