[Influence of dexamethasone on fusion of embryonic palatal medial edge epithelium in mouse palatal shelves in vitro].
OBJECTIVE
To study the effect of dexamethasone on the differentiation and proliferation of type A mouse palatal medial edge epithelial cells when there is type A mouse embryonic palatal mesenchymal cells.


METHODS
The mouse palatal shelves were harvested from a female mouse of gestation day 14 by microsurgical dissection and cultured in vitro. The differentiation was investigated through microscope and transmission electron microscope under condition of the palatal shelves fusion.


RESULTS
Dexamethasone promoted the palatal medial edge epithelium differentiated into squamause epithelium and affected normal development and obstructed the fusion of mouse palatal shelves.


CONCLUSIONS
The results of histological observation indicate that dexamethasone promotes the proliferation of palatal meseuchymal cells and inhibits the normal differentiation of palatal medial edge epithelial cells, which results in cleft palate.
Huang
Lei
L
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Shi
Bing
B
Sun
Jin-Hu
JH
Wang
Yan
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002972:Cleft Palate / Q000139:chemically induced*; D003907:Dexamethasone / Q000009:adverse effects / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium; D005260:Female; D005938:Glucocorticoids / Q000009:adverse effects / Q000494:pharmacology*; D051379:Mice; D009924:Organ Culture Techniques; D021362:Palate, Hard / Q000187:drug effects / Q000196:embryology*
D005938:Glucocorticoids; D003907:Dexamethasone