Reactive oxygen species mediate lethality induced by far-UV in Escherichia coli cells.
The involvement of reactive oxygen species (ROS) in the induction of DNA damage to Escherichia coli cells caused by UVC (254 nm) irradiation was studied. We verified the expression of the soxS gene induced by UVC (254 nm) and its inhibition by sodium azide, a singlet oxygen (1O2) scavenger. Additional results showed that a water-soluble carotenoid (norbixin) protects against the lethal effects of UVC. These results suggest that UVC radiation can also cause ROS-mediated lethality.
Gomes
A A
AA
Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, Brazil.
Silva-Júnior
A C T
AC
Oliveira
E B
EB
Asad
L M B O
LM
Reis
N C S C
NC
Felzenszwalb
I
I
Kovary
K
K
Asad
N R
NR
D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects*; D029968:Escherichia coli Proteins / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D010100:Oxygen / Q000737:chemistry; D017382:Reactive Oxygen Species; D019810:Sodium Azide / Q000737:chemistry / Q000494:pharmacology; D015534:Trans-Activators / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D015534:Trans-Activators; D014157:Transcription Factors; D014867:Water; C070724:SoxR protein, Bacteria; C070725:SoxS protein, E coli; D002338:Carotenoids; D019810:Sodium Azide; C099823:norbixin; D010100:Oxygen