Recombinant rabies virus vaccine strain SAD-l16 inoculated intracerebrally in young mice produces a severe encephalitis with extensive neuronal apoptosis.
Seven-day-old ICR mice were infected by intracerebral inoculation with recombinant rabies virus vaccine strain SAD-L16. Infected mice developed severe and fatal encephalitis with rabies virus-infected neurons in widespread regions of the brain. There was extensive neuronal death with predominant features of apoptosis, as assessed by light and electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining, and immunohistochemical staining for activated caspase-3. Although SAD-L16 is a neuroattenuated rabies virus, it is fully capable of spreading efficiently and inducing widespread neuronal apoptosis in the immature mouse brain.
Rasalingam
Pamini
P
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario.
Rossiter
John P
JP
Jackson
Alan C
AC
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D017209:Apoptosis; D001921:Brain / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron / Q000662:veterinary; D009336:Necrosis; D009474:Neurons / Q000473:pathology* / Q000821:virology; D011818:Rabies / Q000517:prevention & control / Q000662:veterinary; D011819:Rabies Vaccines / Q000633:toxicity*; D011820:Rabies virus / Q000276:immunology / Q000472:pathogenicity*; D011897:Random Allocation; D014614:Vaccines, Synthetic / Q000633:toxicity
D000956:Antigens, Viral; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases