Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.
Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA-SE fluorescent dye on labeled cells. Finally, we determined the optimal staining time of CFDA-SE fluorescent dye for cell labeling. The results show that the optimal concentration of CFDA-SE fluorescent dye for cell labeling varies according to different cell types. CFDA-SE fluorescent dye is non-toxic to cells as the cell death rate caused by CFDA-SE labeling is below 5%. The optimal cell labeling time was determined to be 8 min of incubation with CFDA-SE fluorescent dye. We concluded that the advantages of using CFDA-SE fluorescent dye for cell labeling are as follows: (1) the binding of CFDA-SE fluorescent dye to cells is stable; (2) CFDA-SE fluorescent dye is not toxic and does not modify the viability of labeled cells; and (3) CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling.
Wang
Xiao-Qi
XQ
The Central Laboratory, the First Teaching Hospital of Jilin University, Changchun 130021, China. wangxiaoqi1979@yahoo.com.cn
Duan
Xiu-Mei
XM
Liu
Li-Hua
LH
Fang
Yan-Qiu
YQ
Tan
Yan
Y
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003584:Cytological Techniques; D005434:Flow Cytometry; D005452:Fluoresceins / Q000737:chemistry / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D013194:Staining and Labeling / Q000379:methods*; D013388:Succinimides / Q000737:chemistry / Q000633:toxicity
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides