title: Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells.
abstract: We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Hyogo 661-0974, Japan.
authors.forename: Mohammed Omedul
authors.initials: MO
authors.lastname: Islam
authors.forename: Yonehiro
authors.initials: Y
authors.lastname: Kanemura
authors.forename: Jesmin
authors.initials: J
authors.lastname: Tajria
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shofuda
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
authors.forename: Mami
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D015536:Down-Regulation; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013234:Stem Cells / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D012333:RNA, Messenger