An experimental animal model of fulminant hepatic failure in pigs.
The objective of this study was to develop an experimental animal model of fulminant hepatic failure to test the efficacy of the bioartificial liver system. The portal vein and the hepatic artery were clamped intermittently and then the hepatic artery was ligated (ligation group, n=5). Pigs whose hepatic arteries were not ligated after clamping were assigned to the non-ligation group (n=5). The biochemical changes in blood, histologic alterations of the liver and neurologic examination for pigs were checked up. All animals died within 17 hr in the ligation group. On the other hand, all animals survived more than 7 days in the non-ligation group. In the ligation group, the levels of ammonia, lactic acid and creatinine showed a progressively increasing pattern. Prothrombin time was also prolonged gradually. Cytoplasmic condensation and nuclear pyknosis of hepatocytes were detected histologically at autopsy. Neurologic findings such as decreased pain sensation, tachypnea and no light reflex of pupils were observed. The findings shown in the ligation group are similar to the clinical features of fulminant hepatic failure in human and this animal model is reproducible. Therefore, this can be a suitable animal model to evaluate the efficacy of the bioartificial liver system for treating fulminant hepatic failure.
Lee
Kuhn Uk
KU
Department of Surgery, Seoul National University College of Medicine, Korea. kulee@plaza.snu.ac.kr
Zheng
Long-xian
LX
Cho
Yong Beom
YB
Kim
Ki-Ho
KH
Ha
Jongwon
J
Suh
Kyung-Suk
KS
Jung
Sung Eun
SE
D000138:Acidosis / Q000209:etiology / Q000517:prevention & control; D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D006499:Hepatic Artery / Q000601:surgery; D019344:Lactic Acid / Q000097:blood; D008026:Ligation / Q000009:adverse effects; D017114:Liver Failure, Acute / Q000097:blood / Q000473:pathology*; D011169:Portal Vein / Q000601:surgery; D011188:Potassium / Q000097:blood; D011517:Prothrombin Time; D017693:Sodium Bicarbonate / Q000494:pharmacology; D013552:Swine
D001786:Blood Glucose; D019344:Lactic Acid; D000641:Ammonia; D017693:Sodium Bicarbonate; D003404:Creatinine; D001219:Aspartate Aminotransferases; D001663:Bilirubin; D011188:Potassium