Identification of a second collagen-like glycoprotein produced by Bacillus anthracis and demonstration of associated spore-specific sugars.
Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.
Waller
Lashanda N
LN
Department of Pathology and Microbiology, University of South Carolina School of Medicine, Columbia, South Carolina 29208, USA.
Stump
Michael J
MJ
Fox
Karen F
KF
Harley
William M
WM
Fox
Alvin
A
Stewart
George C
GC
Shahgholi
Mona
M
D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013171:Spores, Bacterial / Q000378:metabolism
D002241:Carbohydrates; D006023:Glycoproteins