title: Preparative separation of isovitexin and isoorientin from Patrinia villosa Juss by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v) was used to isolate and separate two C-glycosylflavones from Patrinia villosa Juss, a traditional Chinese medicine. The separation produced 42.9 mg isovitexin and 20.1 mg isoorientin with purities of 99.3% and 98.5%, respectively as determined by high-performance liquid chromatography (HPLC) in one step elution from 250 mg crude extract, and identification was performed by MS, 1H NMR and 13C NMR. It is the first report of discovering isovitexin and isoorientin from the plant of Patrinia genus.
authors.affiliation: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Hong
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
authors.forename: Yutian
authors.initials: Y
authors.lastname: Wu
mesh_terms: D047310:Apigenin / Q000302:isolation & purification*; D003377:Countercurrent Distribution; D047311:Luteolin / Q000302:isolation & purification*; D032405:Patrinia / Q000737:chemistry*
chemical_list: D047310:Apigenin; C057912:homoorientin; C049772:isovitexin; D047311:Luteolin