title: Pathogenomics analysis of Leishmania spp.: flagellar gene families of putative virulence factors.
abstract: The trypanosomatid flagellar apparatus contains conventional and unique features, whose roles in infectivity are still enigmatic. Although the flagellum and the flagellar pocket are critical organelles responsible for all vesicular trafficking between the cytoplasm and cell surface, still very little is known about their roles in pathogenesis and how molecules get to and from the flagellar pocket. The ongoing analysis of the genome sequences and proteome profiles of Leishmania major and L infantum, Trypanosoma cruzi, T. brucei, and T. gambiensi ( www.genedb.org ), coupled with our own work on L. chagasi (as part of the Brazilian Northeast Genome Program- www.progene.ufpe.br ), prompted us to scrutinize flagellar genes and proteins of Leishmania spp. promastigotes that could be virulence factors in leishmaniasis. We have identified some overlooked parasite factors such as the MNUDC-1 (a protein involved in nuclear development and genomic fusion) and SQS (an enzyme of sterol biosynthesis), among the described flagellar gene families. A database concerning the results of this work, as well as of other studies of Leishmania and its organelles, is available at http://nugen.lcc.uece.br/LPGate . It will serve as a convenient bioinformatics resource on genomics and pathology of the etiological agents of leishmaniasis.
authors.affiliation: Núcleo de Genômica e Bioinformática, Faculdade de Veterinária, Universidade Estadual do Ceara (UECE), Campus do Itaperi, Fortaleza, Ceara, Brazil. nugen@lcc.uece.br
authors.forename: Diana M
authors.initials: DM
authors.lastname: Oliveira
authors.forename: Joao Jose S
authors.initials: JJ
authors.lastname: Gouveia
authors.forename: Nilo B
authors.initials: NB
authors.lastname: Diniz
authors.forename: Ana Carolina L
authors.initials: AC
authors.lastname: Pacheco
authors.forename: Elton Jose R
authors.initials: EJ
authors.lastname: Vasconcelos
authors.forename: Michely C
authors.initials: MC
authors.lastname: Diniz
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Viana
authors.forename: Thiago D
authors.initials: TD
authors.lastname: Ferreira
authors.forename: Marianna C
authors.initials: MC
authors.lastname: Albuquerque
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Fortier
authors.forename: Allan R S
authors.initials: AR
authors.lastname: Maia
authors.forename: Luis A C
authors.initials: LA
authors.lastname: Costa
authors.forename: João Osmar P
authors.initials: JO
authors.lastname: Melo
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: da Silva
authors.forename: Cezar A
authors.initials: CA
authors.lastname: Walter
authors.forename: Jose O
authors.initials: JO
authors.lastname: Faria
authors.forename: Adriana R
authors.initials: AR
authors.lastname: Tome
authors.forename: Marcos Jose N
authors.initials: MJ
authors.lastname: Gomes
authors.forename: Sonia M P
authors.initials: SM
authors.lastname: Oliveira
authors.forename: Raimundo
authors.initials: R
authors.lastname: Araújo-Filho
authors.forename: Raimundo B
authors.initials: RB
authors.lastname: Costa
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Maggioni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030541:Databases, Genetic; D005407:Flagella / Q000235:genetics* / Q000502:physiology; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D007891:Leishmania / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D020543:Proteome; D015800:Protozoan Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics
chemical_list: D020543:Proteome; D015800:Protozoan Proteins