Assessment of DNA damage in nuclear medicine personnel--comparative study with the alkaline comet assay and the chromosome aberration test.
Despite much research over the last few decades, there still remains considerable uncertainty as to the genetic impact of ionizing radiation on human populations, particularly at low levels. The aim of the present study was to provide data on the genetic hazards due to occupational exposure of low doses of ionizing radiation in nuclear medicine departments. The assessment of primary DNA damage in peripheral blood leukocytes of medical staff was performed using the alkaline comet assay and the data obtained were compared with the results of conventional cytogenetic biodosimetry using the chromosome aberration (CA) test. Altogether 120 subjects (60 exposed and 60 controls) participated in the study. Statistically significant increases in primary DNA damage and increased frequencies of CAs compared to controls were observed. Within the exposed population, significant inter-individual differences in DNA damage were found, indicating differences in genome sensitivity. Age and gender were not confounding factors, while smoking enhanced the levels of primary DNA damage only in control subjects, as revealed by both biomarkers studied. The present study suggests that genotoxic damage results from exposure to chronic low doses of ionizing radiation in nuclear medicine departments. Therefore, the exposed medical personnel should carefully comply with the radiation protection procedures and should minimize radiation exposure where possible to avoid potential genotoxic effects. The results obtained in this study point to the significance of biological indicators providing information on the actual risk to the radiation exposed individuals. According to our results, the alkaline comet assay and CA test are sensitive biomarkers that can be used as additional complements to physical dosimetry for assessing exposure to radiation in nuclear medicine personnel.
Kopjar
Nevenka
N
Institute for Medical Research and Occupational Health, Ksaverska c. 2, HR-10000 Zagreb, Croatia. nkopjar@imi.hr
Garaj-Vrhovac
Vera
V
D000328:Adult; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D009683:Nuclear Medicine; D016273:Occupational Exposure; D012907:Smoking / Q000009:adverse effects