Novel proteomic approaches for tissue analysis.
Proteomics, the global study of protein expression and characteristics, has recently emerged as a key component in the field of molecular analysis. The dynamic nature of proteins, from ion channels to chaperones, presents a challenge, yet the understanding of these molecules provides a rich source of information. When applying proteomic analysis directly to human tissue samples, additional difficulties arise. The following article presents an overview of the current proteomic tools used in the analysis of tissues, beginning with conventional methods such as western blot analysis and 2D polyacrylamide gel electrophoresis. The most current high-throughput techniques being used today are also reviewed. These include protein arrays, reverse-phase protein lysate arrays, matrix-assisted laser desorption/ionization, surface-enhanced laser desorption/ionization and layered expression scanning. In addition, bioinformatics as well as issues regarding tissue preservation and microdissection to obtain pure cell populations are included. Finally, future directions of the tissue proteomics field are discussed.
Tangrea
Michael A
MA
Laboratory of Pathology, National Cancer Institute, 8717 Grovemont Circle, Gaithersburg, MD 20877, USA. tangream@mail.nih.gov
Wallis
Benjamin S
BS
Gillespie
John W
JW
Gannot
Gallya
G
Emmert-Buck
Michael R
MR
Chuaqui
Rodrigo F
RF
D000818:Animals; D046228:Microarray Analysis / Q000379:methods; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics / Q000379:methods / Q000639:trends*; D012313:RNA / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome; D012313:RNA