title: [Osteogenic differentiation of synovial mesenchymal stem cells in vitro].
abstract: OBJECTIVE
  To investigate the potential of synovial mesenchymal stem cells (SMSC) in osteogenic differentiation.
  
  
  METHODS
  SMSC were obtained by limited dilution method and expanded to culture in 25-milliliter flasks. The attached cells were treated with inductive medium containing dexamethasone, glycerophosphate and vitamin C at 3rd passage SMSC. The mineralized nodule was stained by Von Kossa method. The expression of ALP and osteopontin were detected by histochemical, immunohistological staining technique, respectively, while the expression of cbfa1 mRNA by RT-PCR.
  
  
  RESULTS
  Pure SMSC which were of spindle shape and star shape, uniform in size, could be induced to pleomorphism osteoblast in vitro, which were intensive positive in ALP and osteopontin. The expression of cbfa1 mRNA were also verified by RT-PCR and the polygonal cells formed nodular structure at 4 weeks. All these were coincident with the characters of osteoblast.
  
  
  CONCLUSIONS
  SMSC can be purified and induced into osteoblast in vitro.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Long
authors.forename: Fan
authors.initials: F
authors.lastname: Zhu
authors.forename: Xue-Chao
authors.initials: XC
authors.lastname: Yang
mesh_terms: D001205:Ascorbic Acid; D002454:Cell Differentiation; D003907:Dexamethasone; D005994:Glycerophosphates; D006801:Humans; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D005994:Glycerophosphates; D012333:RNA, Messenger; D053495:Osteopontin; D003907:Dexamethasone; D001205:Ascorbic Acid