Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.
PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.
Adams
D Nicholas
DN
Department of Microbiology, Royal Prince Alfred Hospital, Missenden Rd., Camperdown 2050, NSW, Australia. nick.adams@email.cs.nsw.gov.au
D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D004269:DNA, Bacterial