Seladin-1/DHCR24 expression in normal ovary, ovarian epithelial and granulosa tumours.
OBJECTIVE
The human DIMINUTO/DWARF1 homolog seladin-1/DHCR24 has been recently reported to be up-regulated in adrenocortical adenomas. Seladin-1 expression has been reported in the normal ovary. Granulosa cell tumours of the ovary (GCT) as with adrenocortical adenomas arise from a steroidogenic tissue, respond to pituitary hormone stimulation and synthesize steroid hormones.


METHODS
To test the hypothesis that seladin-1 may also have a role in the pathogenesis of GCT, we determined the expression of seladin-1 in a cohort of GCT and in mucinous and serous cystadenocarcinomas and in normal ovary.


METHODS
Expression was determined by RT-PCR using gene specific primers and probes combined with Southern blot analysis of the PCR products.


RESULTS
Seladin-1 expression was identified in the normal ovary, mucinous cystadenocarcinomas and serous cystadenocarcinomas of the ovary whereas no expression was observed in the GCT.


CONCLUSIONS
Based on our results, seladin-1 is not expressed in the granulosa cells or at least not in those that give rise to GCT.
Fuller
Peter J
PJ
Prince Henry's Institute of Medical Research, Department of Gynaecology Oncology, Monash Medical Centre, Clayton, Victoria 3168, Australia. peter.fuller@phimr.monash.edu.au
Alexiadis
Maria
M
Jobling
Tom
T
McNeilage
Jane
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern / Q000379:methods; D018282:Cystadenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006106:Granulosa Cell Tumor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics* / Q000378:metabolism; D011974:Receptors, LH / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011974:Receptors, LH; D044925:Oxidoreductases Acting on CH-CH Group Donors; C437069:3beta-hydroxysterol delta24-reductase; C499055:DHCR24 protein, human