title: Phylogenetic relationships and evolutionary traits in Ranunculus s.l. (Ranunculaceae) inferred from ITS sequence analysis.
abstract: Ranunculus is a large genus with a worldwide distribution. Phylogenetic analyses of c. 200 species of Ranunculus s.l. based on sequences of the nrITS using maximum parsimony and Bayesian inference yielded high congruence with previous cpDNA restriction site analyses, but strongly contradict previous classifications. A large core clade including Ranunculus subg. Ranunculus, subg. Batrachium, subg. Crymodes p.p., Ceratocephala, Myosurus, and Aphanostemma is separated from R. subg. Ficaria, subg. Pallasiantha, subg. Coptidium, subg. Crymodes p.p., Halerpestes, Peltocalathos, Callianthemoides, and Arcteranthis. Within the core clade, 19 clades can be described with morphological and karyological features. Several sections are not monophyletic. Parallel evolution of morphological characters in adaptation to climatic conditions may be a reason for incongruence of molecular data and morphology-based classifications. In some mountainous regions, groups of closely related species may have originated from adaptive radiation and rapid speciation. Split decomposition analysis indicated complex patterns of relationship and suggested hybridization in the apomictic R. auricomus complex, R. subg. Batrachium, and the white-flowering European alpines. The evolutionary success of the genus might be due to a combination of morphological plasticity and adaptations, hybridization and polyploidy as important factors for regional diversification, and a broad range of reproductive strategies.
authors.affiliation: Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University of Vienna, Rennweg 14, A-1030 Vienna, Austria. elvira.hoerandl@univie.ac.at
authors.forename: Elvira
authors.initials: E
authors.lastname: Hörandl
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Paun
authors.forename: Jan T
authors.initials: JT
authors.lastname: Johansson
authors.forename: Carlos
authors.initials: C
authors.lastname: Lehnebach
authors.forename: Tristan
authors.initials: T
authors.lastname: Armstrong
authors.forename: Lixue
authors.initials: L
authors.lastname: Chen
authors.forename: Peter
authors.initials: P
authors.lastname: Lockhart
mesh_terms: D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D019143:Evolution, Molecular; D010802:Phylogeny; D031947:Ranunculus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer