Genes transactivated by hepatitis C virus core protein, a microarray assay.
OBJECTIVE
To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.


METHODS
Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.


RESULTS
The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method.


CONCLUSIONS
HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.
Liu
Min
M
Department of Infectious Diseases, The First Affilated, Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. liumin3262@sohu.com
Zhang
Shu-Lin
SL
Cheng
Jun
J
Liu
Yan
Y
Wang
Lin
L
Shao
Qing
Q
Zhang
Jian
J
Lin
Shu-Mei
SM
D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D015967:Gene Expression Regulation, Viral; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000503:physiopathology*; D006801:Humans; D008113:Liver Neoplasms; D020411:Oligonucleotide Array Sequence Analysis