title: One in vitro model for visceral adipose-derived fibroblasts in chronic inflammation.
abstract: One pathogenesis of the obesity-associated complications is that consistent with increased body fat mass, the elevation of adipose tissue-derived cytokines inflicts a low-grade chronic inflammation, which ultimately leads to metabolic disorders. Adipocytes and macrophages in visceral adipose (VA) have been confirmed to contribute to the chronic inflammation; however, the role of the resident fibroblasts is still unknown. We established one VA fibroblast cell line, termed VAFC. Morphological analysis indicated that there were large numbers of pits at the cell plasma membrane. In vitro VAFC cells promoted bone marrow cells to differentiate into macrophages and protected them from apoptosis in the serum-free conditions. Additionally, they also interfered in lymphocytes proliferation. On the basis of these results, this cell line might be an in vitro model for understanding the role of adipose-derived fibroblasts in obesity-associated chronic inflammation.
authors.affiliation: Laboratory of Biochemistry, National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Yue
authors.forename: Li-Rui
authors.initials: LR
authors.lastname: Du
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: Xian-Hui
authors.initials: XH
authors.lastname: He
authors.forename: Huan
authors.initials: H
authors.lastname: Qiu
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Xu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Sheng-Qiu
authors.initials: SQ
authors.lastname: Feng
authors.forename: Zai-Qing
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D002908:Chronic Disease; D017931:DNA Primers; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D020869:Gene Expression Profiling; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009765:Obesity / Q000473:pathology
chemical_list: D017931:DNA Primers