title: Sporicidal activity in liquid chemical products to sterilize or high-level disinfect medical and dental instruments.
abstract: BACKGROUND
  Various liquid chemical products are commercially available in Mexico under sporicidal label claims. Frequently, information provided on their labels conflicts with published data on active ingredients, use concentrations, or exposure times.
  
  
  OBJECTIVE
  To evaluate sporicidal activity in 8 chemical products sold for the sterilization or high-level disinfection of medical and dental instruments.
  
  
  METHODS
  Bacillus atrophaeus ATCC 9372 spores were suspended (10 6 spores in 50 mL) in each of 4 glutaraldehyde solutions: a super oxidized solution, a hydrogen peroxide formulation, a quaternary ammonium compound, and an ortho -phthalaldehyde solution. After up to a 10-hour exposure, the solutions were passed through 0.22-mum filters, which were then rinsed with 1% sodium bisulfite, washed, and incubated on tryptic soy agar for 5 days at 37 degrees C.
  
  
  RESULTS
  At the use concentrations stipulated on their labels, only 2 of 6 products registered as sterilants showed sporicidal activity when challenged with 6 log 10 : a 2% glutaraldehyde in 10 hours and the hydrogen peroxide solution in 6 hours. Of 2 products registered as high-level disinfectants, only the ortho -phthalaldehyde showed noticeable sporicidal activity after 10-hour exposure.
  
  
  CONCLUSIONS
  The results show that some chemical products, commercially available in Mexico as "Liquid Chemical Sterilants/High-Level Disinfectants" cannot be used reliably to process instruments between patients.
authors.affiliation: Microbiology and Infection Control Laboratory, Postgraduate Division, National University of Mexico Dental School, Mexico City, 04510 Mexico. acostag@servidor.uam.mx
authors.forename: A Enrique
authors.initials: AE
authors.lastname: Acosta-Gío
authors.forename: José L
authors.initials: JL
authors.lastname: Rueda-Patiño
authors.forename: Leonor
authors.initials: L
authors.lastname: Sánchez-Pérez
mesh_terms: D004202:Disinfectants; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D008800:Mexico; D013171:Spores, Bacterial; D013242:Sterilization / Q000379:methods*
chemical_list: D004202:Disinfectants