[The degree of cerebal oxidative stress in rats during ischemia-reperfusion combined with a modulaion of L-arginine-NO system].
We estimated the cerebral oxidative stress during ischemia-reperfusion of the brain in conditions of modulated L-arginine-NO system in rats. In 128 rats, the changes in lipid peroxidation products (conjugated dienes, malondialdehyde, Schiff bases) and antioxidant defense factors (retinal, a-tocopherol, SH-groups) were studied. Analysis of the inhibitor (administration of NO synthase substrate L-arginine, non-selective inhibitor Nw-nitro-L-arginine methyl ester, selective inhibitor of neuronal NO synthase 7-nitroindasole, or selective inhibitor of inducible NO synthase S-methyl-isothiourea) showed the NO-dependent nature of oxidative stress in rats with brain ischemia-reperfusion. NO generated by different NO synthase isoenzymes played different roles: hyperactivation of the neuronal NO synthase was associated with oxidative stress during both brain ischemia-reperfusion periods, and the higher inducible NO synthase activity had such association only during the later period.
Maksimovich
N E
NE
Zinchuk
V V
VV
Maslakov
D A
DA
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D018384:Oxidative Stress; D051381:Rats; D015427:Reperfusion Injury / Q000201:enzymology / Q000378:metabolism*; D012545:Schiff Bases / Q000032:analysis; D024502:alpha-Tocopherol / Q000032:analysis
D004791:Enzyme Inhibitors; D012545:Schiff Bases; D008315:Malondialdehyde; D001120:Arginine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D024502:alpha-Tocopherol; D019331:NG-Nitroarginine Methyl Ester