Phase II metabolites of etofesalamide in filamentous fungi.
OBJECTIVE
To study phase II metabolites of etofesalamide in filamentous fungi.


METHODS
Seven fungi were screened to transform etofesalamide. The metabolites of etofesalamide were assayed using liquid chromatography coupled to mass spectrometry. The major metabolite was subject to enzymatic hydrolysis to confirm its structure.


RESULTS
Etofesalamide was converted into two phase II metabolites: glucoside and riboside conjugates. Glucoside conjugate was the major product with a yield greater than 90%; no phase I metabolites were detected.


CONCLUSIONS
Glucoside and riboside conjugations of etofesalamide in filamentous fungi differ from the phase II metabolism of glucuronidation in mammals.
Huang
Hai-Hua
HH
Department of Pharmaceutical Microbiology, Shenyang Pharmaceutical University, Shenyang 110016, China.
Ma
Gui-Lei
GL
Sun
Yu-Ming
YM
Li
Qiang
Q
Zhong
Da-Fang
DF
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D001230:Aspergillus / Q000378:metabolism; D001549:Benzamides / Q000378:metabolism*; D001711:Biotransformation; D002853:Chromatography, Liquid; D020093:Cunninghamella / Q000145:classification / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism; D013058:Mass Spectrometry; D009089:Mucor / Q000378:metabolism; D010407:Penicillium / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001549:Benzamides; D005960:Glucosides; C512377:etofesalamide