Mouse hepatitis virus 3 binding to macrophages correlates with resistance to experimental infection.
Mouse hepatitis virus 3 (MHV3) infection of A/J and BALB/c mice has been used as a model of resistance/susceptibility. A/J mice recover from a mild disease after 4-6 days of infection and the BALB/c mice develop an acute hepatitis and die after 3-4 days of infection. In view of studying the MHV3 binding to cells or cell extracts, we performed an enzyme-linked immunosorbent assay-like virus-binding assay, preparing microplates with L929 cells, A/J or BALB/c mouse macrophages and also with proteins extracted from these cells. Higher MHV3 bindings were observed to proteins of BALB/c macrophages than to the A/J ones. The interferon-gamma (IFN-gamma) activation led to a reduction of MHV3 binding only to proteins of resistant A/J mouse macrophages. Our experiments contribute to the hypothesis that IFN-gamma activation of macrophages plays an important role against MHV3 infection by downregulating the expression of viral receptors.
Pereira
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CA
Laboratório de Imunologia Viral, Instituto Butantan, Sao Paulo, Brazil. grugel@butantan.gov.br
Moreira
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C
Tsuhako
M H
MH
de Franco
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MT
D000818:Animals; D018352:Coronavirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D049673:History, 20th Century; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology*
D007371:Interferon-gamma