Potential mechanism of blood vessel protection by resveratrol, a component of red wine.
Resveratrol-mediated heme oxgenase-1 (HO-1) induction has been shown to occur in primary neuronal cultures and has been implicated as having potential neuroprotective action. Further, antioxidant properties of resveratrol have been reported to protect against coronary heart disease. We attempted to examine the HO-1 inducing potency of resveratrol and the regulatory mechanism of its induction in rat aortic smooth muscle cells (RASMC). We showed that resveratrol-induced HO-1 expression was concentration- and time-dependent. The level of HO-1 expression and its promoter activity mediated by resveratrol was attenuated by nuclear factor-kappa B (NF-kappaB) inhibitors, but not by mitogen-activated protein kinase (MAPK) inhibitors. Deletion of NF-kappaB binding sites in the promoter region strongly reduced luciferase activity. Collectively, we suggest that resveratrol-mediated HO-1 expression occurs, at least in part, through the NF-kappaB pathway.
Huang
Huei-Mei
HM
Graduate Institute of Cell and Molecular Biology, Center for Stem Cells Research at Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan.
Liang
Yu-Chih
YC
Cheng
Tzu-Hurng
TH
Chen
Cheng-Heien
CH
Juan
Shu-Hui
SH
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003116:Color; D024202:Electrophoretic Mobility Shift Assay; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014920:Wine
D016328:NF-kappa B; D047428:Protein Kinase Inhibitors; D013267:Stilbenes; D051547:Heme Oxygenase-1; D020928:Mitogen-Activated Protein Kinases; D000077185:Resveratrol