[c-myc gene silencing in K562 cells with RNA interference].
OBJECTIVE
To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells.


METHODS
siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting.


RESULTS
Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate.


CONCLUSIONS
RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.
Yue
Feng
F
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. yuefeng@fimmu.com
Ma
Wen-li
WL
Song
Yan-bin
YB
Shi
Rong
R
Zhang
Bao
B
Zheng
Wen-ling
WL
D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D020868:Gene Silencing; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering
D034741:RNA, Small Interfering