title: Evaluation of hybridisation on oligonucleotide microarrays for analysis of drug-resistant Mycobacterium tuberculosis.
abstract: A molecular approach was developed to identify drug-resistant strains of Mycobacterium tuberculosis by means of biochips with oligonucleotides immobilised in polyacrylamide gel pads. The technique was based on multiplex PCR, followed by hybridisation on an oligonucleotide microarray, and detected > 95% of rifampicin-resistant and c. 80% of isoniazid-resistant M. tuberculosis isolates within 12 h. In total, 220 drug-resistant isolates and 131 clinical samples were tested using biochips. The sensitivity and specificity of the developed method were comparable with those of standard bacteriological testing of M. tuberculosis drug resistance.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. grad@redline.ru
authors.forename: D
authors.initials: D
authors.lastname: Gryadunov
authors.forename: V
authors.initials: V
authors.lastname: Mikhailovich
authors.forename: S
authors.initials: S
authors.lastname: Lapa
authors.forename: N
authors.initials: N
authors.lastname: Roudinskii
authors.forename: M
authors.initials: M
authors.lastname: Donnikov
authors.forename: S
authors.initials: S
authors.lastname: Pan'kov
authors.forename: O
authors.initials: O
authors.lastname: Markova
authors.forename: A
authors.initials: A
authors.lastname: Kuz'min
authors.forename: L
authors.initials: L
authors.lastname: Chernousova
authors.forename: O
authors.initials: O
authors.lastname: Skotnikova
authors.forename: A
authors.initials: A
authors.lastname: Moroz
authors.forename: A
authors.initials: A
authors.lastname: Zasedatelev
authors.forename: A
authors.initials: A
authors.lastname: Mirzabekov
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin