Expression of BLT1 leukotriene B4 receptor on the dorsal root ganglion neurons in mice.
We investigated using mice whether dorsal root ganglion (DRG) neurons express leukotriene B4 receptors. BLT1, but not BLT2, receptor mRNA was expressed in the DRG. Leukotriene B4 increased intracellular Ca2+ concentration in cultured DRG neurons, which was inhibited by leukotriene B4 antagonist. Many BLT1-immunoreactive neurons were small in size and positive for TRPV1. BLT1-immunoreactive fibers are present in the skin. The results suggest the expression of functional BLT1 receptors in the sensory neurons.
Andoh
Tsugunobu
T
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Kuraishi
Yasushi
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology; D048429:Cell Size; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D018102:Receptors, Leukotriene B4 / Q000235:genetics* / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000378:metabolism; D012867:Skin / Q000294:innervation; D050916:TRPV Cation Channels
D007473:Ion Channels; C503704:Ltb4r1 protein, mouse; D012333:RNA, Messenger; D018102:Receptors, Leukotriene B4; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D007975:Leukotriene B4; D002118:Calcium