Inhibitory effect on proliferation of vascular smooth muscle cells and protective effect on CCl(4)-induced hepatic damage of HEAI extract.
The effects of methanol extract from Hericium erinaceus cultivated with Artemisia iwayomogi (HEAI) on proliferation of vascular smooth muscle cells and CCl(4)-induced hepatic damage were evaluated. HEAI was shown to have a potent inhibitory effect on the proliferation of vascular smooth muscle cells (VSMCs). Interestingly, a methanol extract of Hericium erinaceus showed no inhibitory effect on the proliferation of VSMCs, while a methanol extract of Artemisia iwayomogi possessed strong inhibitory effects on the proliferation of VSMCs. Therefore, the inhibitory effects of HEAI may be caused by the changes of chemical components in the culture broth after the addition of Artemisia iwayomogi in the HEAI growth media. HEAI also had a strong protective effect on CCl(4)-induced hepatic damage in rats. The activity was evaluated using biochemical parameters such as glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and alkaline phosphatase (ALP). HEAI treatment caused a significant reduction in the activity of GOT but not of GPT and ALP in comparison with CCl(4) treatment alone. Histopathological studies showed that liver samples treated with HEAI were significantly different when compared to non-treated animals after CCl(4) exposure.
Choi
Won-Sik
WS
Department of Life Sciences, Soonchunhyang University, Asan, Chungnam 336-745, Korea.
Kim
Chang-Jin
CJ
Park
Byeoung-Soo
BS
Lee
Sung-Eun
SE
Takeoka
Gary R
GR
Kim
Dong-Goo
DG
Lanpiao
Xiang
X
Kim
Jeong-Han
JH
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors; D049109:Cell Proliferation / Q000187:drug effects*; D056486:Chemical and Drug Induced Liver Injury; D004305:Dose-Response Relationship, Drug; D008107:Liver Diseases / Q000188:drug therapy*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
D010936:Plant Extracts; D002251:Carbon Tetrachloride