Interleukin-2alpha receptor in membrane lipid rafts.
Acute allograft rejection is driven by production of cytokines such as interleukin(IL)-2 that activate and expand alloreactive T cells by ligating high-affinity IL-2alpha receptors. Lipid rafts on the membranes of T cells may be the functional microdomains for IL-2alpha receptor. Flow cytometric analysis was performed for expression of CD25 (IL-2alpha receptor) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Ralpha in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Cells were stimulated with IL-2 and expression of CD25 (IL-2alpha receptor) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Ralpha was localized in lipid rafts. Lipid rafts are the functional microdomains for IL-2alpha receptors.
Li
Q-R
QR
Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, People's Republic of China. liqiurong@yahoo.com
Ma
J
J
Wang
H
H
Li
J-S
JS
D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D053645:Interleukin-2 Receptor alpha Subunit; D019169:Jurkat Cells; D021962:Membrane Microdomains / Q000276:immunology*; D018123:Receptors, Interleukin / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D018123:Receptors, Interleukin