Immortalized human microglial cell line: phenotypic expression.
Microglia are a major neuroglial component of the CNS, playing an important role as resident immunocompetent and phagocytic cells in the CNS in the event of injury and disease. To understand the role of microglia in the CNS in health and diseases, we have recently established an immortalized clonal cell line of human microglia, HMO6, from human embryonic telencephalon tissue by using a retroviral vector encoding v-myc. This immortalized microglia HMO6 cell line exhibits cell-type-specific antigens for microglia, including CD11b (Mac-1), CD68, CD86 (B7-2), HLA-ABC, HLA-DR, and RCA-1 lectin, and actively phagocytoses latex beads.
Nagai
Atsushi
A
Department of Neurology, Shimane University School of Medicine, Izumo, Japan.
Mishima
Seiji
S
Ishida
Yuri
Y
Ishikura
Hiroto
H
Harada
Takayuki
T
Kobayashi
Shotai
S
Kim
Seung U
SU
D000818:Animals; D000938:Antigen-Presenting Cells / Q000378:metabolism; D002460:Cell Line; D017690:Cell Transplantation; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000502:physiology; D010641:Phenotype
D018925:Chemokines; D016207:Cytokines