[Identification of the related gene of mouse tolerant to exposure to perfluoroisobutylene by DDRT-PCR].
OBJECTIVE
The mice surviving acute exposure to low dose of perfluoroisobutylene (PFIB) could tolerant to lethal dose of PFIB. Discovering the gene related with the tolerance would be significant to find preventive agents for PFIB.


METHODS
By static exposure to PFIB for 3 times, the survival animals could tolerate to lethal dose of PFIB. Three anchoring primers and 4 arbitrary primers were applied in differential display reverse transcription PCR (DDRT-PCR) of lung tissues and the products were analyzed by sequencing PAGE and silver staining. The differential DNA fragments were cloned and sequenced.


RESULTS
There were obvious differences of gene expression between control and tolerant animals. Three expressed sequence tags (EST) have been cloned and sequenced,which were homologous to some mouse gene in Genbank.


CONCLUSIONS
Specific gene up-regulation might be the cause of tolerance to PFIB, and they are very important to clarity the tolerant mechanisms to PFIB.
Tang
Zhi-hua
ZH
Nanjing Military Medical Institute, Nanjing 210002, China.
Rong
Shu
S
Ding
Shu-biao
SB
D000818:Animals; D004361:Drug Tolerance / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D005466:Fluorocarbons / Q000506:poisoning*; D015870:Gene Expression; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D005466:Fluorocarbons; D012333:RNA, Messenger; C016648:perfluoroisobutylene