title: Embryo production from superovulated cattle following insemination of sexed sperm.
abstract: Two trials were conducted to ascertain fertilization rate, embryo quality and numbers of transferable embryos in superovulated heifers and cows inseminated with sexed sperm. Inseminates contained 2 x 10(6), 10 x 10(6) or 20 x 10(6) total sperm enriched for the X- or Y-chromosome ( approximately 90%) by flow cytometry/cell sorting. Non-sexed inseminates contained 40 x 10(6) total sperm. Donors in each trial were allocated to one of each of the bulls included in that study. Each donor was inseminated with frozen/thawed sperm from the same bull for each treatment in successive courses of superstimulation with twice daily i.m. injections of FSH for 4 d. Heifers and cows were inseminated 12 and 24 h after visually observed standing estrus in Trial 1. In Trial 2, a single timed inseminate was used 70-72 h following PGF(2alpha). Ova/embryos were collected non-surgically 7-7.5 d after insemination. In both trials, fewer ova were fertilized with sexed versus non-sexed treatments and with 2 x 10(6) sexed sperm compared to higher doses (P < 0.05). However, insemination of 20 x 10(6) total sexed sperm of >or=90% purity resulted in similar numbers of transferable embryos of the desired sex compared to that for non-sexed sperm.
authors.affiliation: XY Inc., 2301 Research Blvd., Suite 110, Fort Collins, CO 80526, USA. jschenk@xyinc.com
authors.forename: J L
authors.initials: JL
authors.lastname: Schenk
authors.forename: T K
authors.initials: TK
authors.lastname: Suh
authors.forename: G E
authors.initials: GE
authors.lastname: Seidel
mesh_terms: D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D016018:Least-Squares Analysis; D050498:Live Birth / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D013094:Spermatozoa / Q000145:classification*; D013480:Superovulation / Q000502:physiology*
chemical_list: D005640:Follicle Stimulating Hormone