In vitro activity of the essential oil of Cinnamomum zeylanicum and eugenol in peroxynitrite-induced oxidative processes.
The essential oil obtained from the bark of Cinnamomum zeylanicum Blume (Lauraceae) and three of its main components, eugenol, (E)-cinnamaldehyde, and linalool (representing 82.5% of the total composition), were tested in two in vitro models of peroxynitrite-induced nitration and lipid peroxidation. The essential oil and eugenol showed very powerful activities, decreasing 3-nitrotyrosine formation with IC50 values of 18.4 microg/mL and 46.7 microM, respectively (reference compound, ascorbic acid, 71.3 microg/mL and 405.0 microM) and also inhibiting the peroxynitrite-induced lipid peroxidation showing an IC50 of 2.0 microg/mL and 13.1 microM, respectively, against 59.0 microg/mL (235.5 microM) of the reference compound Trolox. On the contrary, (E)-cinnamaldehyde and linalool were completely inactive.
Chericoni
Silvio
S
Dipartimento di Chimica Bioorganica e Biofarmacia, Università di Pisa, via Bonanno 33, 56126 Pisa, Italy.
Prieto
José M
JM
Iacopini
Patrizia
P
Cioni
Pierluigi
P
Morelli
Ivano
I
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002935:Cinnamomum zeylanicum / Q000737:chemistry*; D005054:Eugenol / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009566:Nitrates / Q000737:chemistry; D009822:Oils, Volatile / Q000494:pharmacology*; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D051381:Rats; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
D009566:Nitrates; D009822:Oils, Volatile; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D005054:Eugenol; D014443:Tyrosine