Influence of the aggregation state in solution on the supramolecular organization of adsorbed type I collagen layers.
In the last years, adsorbed collagen was shown to form layers with a supramolecular organization depending on the substrate surface properties and on the preparation procedure. If the concentration of collagen and the duration of adsorption are sufficient, fibrillar collagen structures are formed, corresponding to assemblies of a few molecules. This occurs more readily on hydrophobic compared to hydrophilic surfaces. This study aims at understanding the origin of such fibrillar structures and in particular at determining whether they result from the deposition of fibrils formed in solution or from the building of assemblies at the interface. Therefore, type I collagen solutions with an increasing degree of aggregation were prepared, using the "neutral-start" approach, by ageing pH 5.8 solutions at 37 degrees C for 15 min, 2 or 7 days. The obtained solutions were used to investigate the influence of collagen aggregation in solution on the supramolecular organization of adsorbed collagen layers, which was characterized by X-ray photoelectron spectroscopy and atomic force microscopy. Polystyrene and plasma-oxidized polystyrene were chosen as substrates for the adsorption. The size and the density of collagen fibrils at the interface decreased upon increasing the degree of aggregation of collagen in solution. This is explained by a competitive adsorption process between monomers and aggregates of the solution, turning at the advantage of the monomers. More aggregated solutions, which are thus depleted in free monomers, behave like less concentrated solutions, i.e. lead to a lower adsorbed amount and less fibril formation at the interface. This study shows that the supramolecular fibrils observed in adsorbed collagen layers, especially on hydrophobic substrates, are not formed in the solution, prior to adsorption, but are built at the interface, through the assembly of free segments of adsorbed molecules.
Dupont-Gillain
Christine C
CC
Unité de Chimie des Interfaces, Université Catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium. dupont@cifa.ucl.ac.be
Jacquemart
Isabelle
I
Rouxhet
Paul G
PG
D000327:Adsorption; D003094:Collagen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D011137:Polystyrenes / Q000737:chemistry; D012996:Solutions; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet
D011137:Polystyrenes; D012996:Solutions; D003094:Collagen