title: Reduced platelet adhesion to titanium metal coated with apatite, albumin-apatite composite or laminin-apatite composite.
abstract: Titanium metal coated with apatite (HA-Ti), albumin-apatite composite (AA-Ti) or laminin-apatite composite (LA-Ti) was prepared by the immersion of NaOH- and heat-treated titanium metal in a calcium phosphate solution, or one supplemented with albumin or laminin. Platelet adhesion to the obtained materials under flow conditions was investigated in real time using a cone- and plate-type viscometer and fluorescence labeled platelets. Adhesion and activation of the platelets on the HA-Ti, AA-Ti and LA-Ti were definitely suppressed as compared with those on untreated titanium metal with a mirror surface. Furthermore, the numbers of platelets adhered to AA-Ti and LA-Ti are smaller than those adhered to HA-Ti, although the differences were not statistically significant. These findings suggest that HA-Ti, AA-Ti and LA-Ti, especially AA-Ti and LA-Ti, would exhibit thromboresistance that is superior to commercially pure titanium metal in terms of platelet adhesion.
authors.affiliation: Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, Tsukuba, Ibaraki, Japan. uchida-m@aist.go.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Uchida
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
authors.forename: Katsuko S
authors.initials: KS
authors.lastname: Furukawa
authors.forename: Keigo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Onimura
authors.forename: Ayako
authors.initials: A
authors.lastname: Oyane
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamane
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tamaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tateishi
mesh_terms: D000328:Adult; D000418:Albumins / Q000737:chemistry / Q000494:pharmacology*; D001031:Apatites / Q000737:chemistry*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000418:Albumins; D001031:Apatites; D020099:Coated Materials, Biocompatible; D007797:Laminin; D014025:Titanium