title: Lentivirus-specific cytotoxic T-lymphocyte responses are rapidly lost in thymectomized cats infected with feline immunodeficiency virus.
abstract: To what extent the thymus is needed to preserve the virus-specific cytotoxic T-lymphocyte (CTL) response of lentivirus-infected adults is unclear. Presented here is the first definitive study using thymectomized (ThX) animals to directly evaluate the contribution of thymic function to lentivirus-specific CTL response and the control of lentivirus infections. ThX and mock-ThX cats were inoculated with feline immunodeficiency virus (FIV) and monitored for their FIV-specific CTL responses. Early in infection, both FIV-ThX and FIV-mock-ThX cats produced similar CTL responses, but surprisingly, after 20 weeks, the FIV-ThX cats showed a statistically significant loss of FIV-specific CTL activity, while FIV-infected cats with intact thymuses continued to maintain FIV-specific CTL. The loss of CTL did not affect plasma virus load, which remained elevated for both groups. These results emphasize the importance of thymic integrity in maintaining immunity to lentiviruses, but also bring into question the notion that virus load is regulated predominantly by the virus-specific CTL response.
authors.affiliation: Department of Veterinary Biosciences, Center for Retrovirus Research, The Ohio State University, 1925 Coffey Road, Columbus, Ohio 43210, USA.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Hayes
authors.forename: Sadi
authors.initials: S
authors.lastname: Köksoy
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Phipps
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Buck
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Kociba
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Mathes
mesh_terms: D000818:Animals; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000302:isolation & purification; D016086:Lentivirus / Q000276:immunology*; D016180:Lentivirus Infections / Q000382:microbiology; D012367:RNA, Viral / Q000097:blood; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013934:Thymectomy; D019562:Viral Load
chemical_list: D012367:RNA, Viral