Doxycycline, the drug used to control the tet-regulatable promoter system, has no effect on global gene expression in Saccharomyces cerevisiae.
The tet-regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is widely accepted that doxycycline is innocuous to yeast. Global genetic studies are now commonplace and the tetO-system is being used in transcriptome studies. Hence, we wanted to ensure that the absence of phenotypic effects, on addition of doxycycline to the growth medium, is mirrored in transcriptome data. We have demonstrated that doxycycline has no significant effect on global transcription levels and will continue to use the tetO-regulatable promoter system for genetic studies.
Wishart
Jill A
JA
Faculty of Life Sciences, University of Manchester, Michael Smith Building, Manchester M13 9PT, UK.
Hayes
Andrew
A
Wardleworth
Leanne
L
Zhang
Nianshu
N
Oliver
Stephen G
SG
D003470:Culture Media; D004318:Doxycycline / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D014158:Transcription, Genetic
D003470:Culture Media; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D013752:Tetracycline; D004318:Doxycycline