title: Positive regulation of sugar catabolic pathways in the cyanobacterium Synechocystis sp. PCC 6803 by the group 2 sigma factor sigE.
abstract: The sigE gene of Synechocystis sp. PCC 6803 encodes a group 2 sigma factor for RNA polymerase and has been proposed to function in transcriptional regulation of nitrogen metabolism. By using microarray and Northern analyses, we demonstrated that the abundance of transcripts derived from genes important for glycolysis, the oxidative pentose phosphate pathway, and glycogen catabolism is reduced in a sigE mutant of Synechocystis maintained under the normal growth condition. Furthermore, the activities of the two key enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, encoded by the zwf and gnd genes were also reduced in the sigE mutant. The dark enhancements in both enzyme activity and transcript abundance apparent in the wild type were eliminated by the mutation. In addition, the sigE mutant showed a reduced rate of glucose uptake and an increased intracellular level of glycogen. Moreover, it was unable to proliferate under the light-activated heterotrophic growth conditions. These results indicate that SigE functions in the transcriptional activation of sugar catabolic pathways in Synechocystis sp. PCC 6803.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Osanai
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Munehiko
authors.initials: M
authors.lastname: Asayama
authors.forename: Makoto
authors.initials: M
authors.lastname: Shirai
authors.forename: Minoru
authors.initials: M
authors.lastname: Kanehisa
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
authors.forename: Norio
authors.initials: N
authors.lastname: Murata
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis; D020411:Oligonucleotide Array Sequence Analysis; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D012808:Sigma Factor; C111259:sigE protein, Bacteria; D006003:Glycogen; D005947:Glucose