title: Enzyme-linked immunosorbent assay for detection of canine chromogranin A by use of immunological cross-reactivity of rabbit anti-bovine chromogranin A antibody.
abstract: Bovine and canine chromogranin A were extracted and purified from each specie's adrenal glands. Isolated bovine 70 kDa protein showed 100% identity to bovine CgA reported previously, whereas isolated canine 68 kDa protein showed 83.3% identity to bovine CgA by the NH(2)-terminal amino acid sequence analysis. Rabbit antibody to purified bovine protein (CgA) was found to immunologically cross-reacted with purified canine protein (CgA). In sandwich ELISA with anti-bovine CgA, concentration-dependent curves were obtained ranging from 0.3 to 20 mug/ml for canine CgA. From these findings, sandwich ELISA with anti-bovine CgA is found to be useful to determine the concentration of canine CgA.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai-shi, Osaka 599-8531, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Akiyoshi
authors.forename: Mica
authors.initials: M
authors.lastname: Aoki
authors.forename: Daijiro
authors.initials: D
authors.lastname: Kumagai
authors.forename: Nahed
authors.initials: N
authors.lastname: Saleh
authors.forename: Katsura
authors.initials: K
authors.lastname: Noda
authors.forename: Terumasa
authors.initials: T
authors.lastname: Shimada
authors.forename: Shunji
authors.initials: S
authors.lastname: Sugii
authors.forename: Fumihito
authors.initials: F
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D000906:Antibodies; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011817:Rabbits; D013045:Species Specificity
chemical_list: D000906:Antibodies; D053379:Chromogranin A; D002864:Chromogranins