[Endovascular microcoil applied for gene delivery system].
OBJECTIVE
To explore the possibility of using an endovascular microcoil as a gene delivery system.


METHODS
Anti-adenoviral monoclonal antibodies were covalently attached to the collagen-coated surface of platinum microcoil. These antibodies were used to tether adenovirus encoding green fluorescent protein (Ad-GFP). Cell culture studies with rat arterial smooth muscle cells (A10) assessed transduction on or near the coil. Platinum coils coated with Ad-GFP were implanted into the ligated common carotid artery (CCA) of adult rats in a model of arterial stasis and pressurization. After 7 days, CCA segments were harvested, and coils were removed for histopathology and GFP expression studies, while organs were evaluated by polymerase chain reaction to assess viral biodistribution.


RESULTS
In cell culture studies, GFP-positive smooth muscle cells were detected only on the platinum coil surface. After 7 days, GFP was detected on the harvested platinum coil and in the organizing thrombus within the CCA according to fluorescence microscopy and immunohistochemistry. Morphometric analyses revealed that (13.3 +/- 2.0)% of cells within the organized thrombus were transduced with Ad-GFP via the gene delivery system. Ad-GFP was not detectable by polymerase chain reaction in lung, liver, or kidney.


CONCLUSIONS
Gene delivery endovascular microcoils represents an interventional device-based gene therapy system that can serve as a suitable platform for either single or multiple gene therapy vectors.
Mei
Lin
L
Tianjin Biomedical Material Key Laboratory, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.
Sun
Hong-fan
HF
Tang
Li-na
LN
Yang
Jing
J
Chang
Jin
J
Song
Cun-xian
CX
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000783:Aneurysm / Q000601:surgery; D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry* / Q000378:metabolism; D001682:Biological Availability; D017536:Carotid Artery, Common / Q000187:drug effects / Q000378:metabolism / Q000601:surgery; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004621:Embolization, Therapeutic / Q000379:methods; D016228:Endothelial Growth Factors / Q000627:therapeutic use; D015316:Genetic Therapy / Q000295:instrumentation* / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000032:analysis; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D010984:Platinum / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000627:therapeutic use; D014161:Transduction, Genetic / Q000295:instrumentation / Q000379:methods
D000914:Antibodies, Viral; D016228:Endothelial Growth Factors; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D010984:Platinum