Differential expression of LFA-3, Fas and MHC Class I on Ad5- and Ad12-transformed human cells and their susceptibility to lymphokine-activated killer (LAK) cells.
Adenovirus (Ad) E1A is a potent oncogene and has been shown to deregulate the expression of a large number of cellular genes leading to cellular transformation. Here we have analysed the expression of several immunomodulatory molecules on the surface of a set of human cell lines transformed with either Ad12 or Ad5. Human cells transformed with Ad12 demonstrated reduced expression of cell surface LFA-3, Fas and MHC class I when compared to Ad5-transformed cells. Furthermore, Ad12-transformed human cell lines demonstrated greater susceptibility to lysis by lymphokine-activated killer (LAK) cells, compared to Ad5-transformed human cell lines. In contrast, previous studies with rodent cells showed that both Ad5- and Ad12-transformed rat cells were susceptible to LAK cells. Thus, transformation of human cells with Ad5 or Ad12 results in differences in the expression of immunomodulatory molecules on the cell surface and differential recognition of these virus-transformed cells by immune effector cells.
Bottley
Graham
G
Molecular Cell Biology Research Group and School of Biochemistry and Microbiology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Cook
Graham P
GP
Meade
Josephine L
JL
Holt
John R
JR
Hoeben
Rob C
RC
Blair
G Eric
GE
D000256:Adenoviridae / Q000235:genetics*; D017500:Adenovirus E1A Proteins / Q000235:genetics; D017501:Adenovirus E1B Proteins / Q000235:genetics; D001483:Base Sequence; D018968:CD58 Antigens / Q000235:genetics*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019014:fas Receptor / Q000235:genetics*
D017500:Adenovirus E1A Proteins; D017501:Adenovirus E1B Proteins; D018968:CD58 Antigens; D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D019014:fas Receptor