title: The Shigella flexneri serotype Y vaccine candidate SFL124 originated from a serotype 2a background.
abstract: Shigella flexneri is endemic in most developing countries and responsible for the highest mortality rate among the Shigella species. The attenuated serotype Y S. flexneri strain SFL124 has been used as the parental strain for the development of recombinant vaccines expressing multiple O-antigen structures. During the development of one such multivalent vaccine, a region of gtrII homology was found in SFL124. Sequencing and analysis of this region revealed the presence of an insertion element interrupted serotype 2a serotype-conversion locus in the serotype Y vaccine strain SFL124. The data presented suggests that SFL124 has derived from a serotype 2a background.
authors.affiliation: Faculty of Science, School of Biochemistry and Molecular Biology, The Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Fleur
authors.initials: F
authors.lastname: Roberts
authors.forename: Amy V
authors.initials: AV
authors.lastname: Jennison
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Verma
mesh_terms: D001483:Base Sequence; D007446:Chromosome Inversion / Q000235:genetics; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000276:immunology / Q000517:prevention & control; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D012703:Serotyping; D022281:Shigella Vaccines / Q000235:genetics / Q000276:immunology*; D012763:Shigella flexneri / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D022281:Shigella Vaccines; D014614:Vaccines, Synthetic