Delayed vaccine virus replication in chickens vaccinated subcutaneously with an immune complex infectious bursal disease vaccine: quantification of vaccine virus by real-time polymerase chain reaction.
The distribution of the immune complex vaccine virus for infectious bursal disease (IBD) in tissue was examined and the viral loads of the organs were quantitatively compared. One-day-old specific pathogen free (SPF) and maternally immune broiler chickens were injected subcutaneously with the vaccine. Lymphoid and non-lymphoid tissues were collected at various time intervals during the experiment to test for infectious bursal disease virus (IBDV)-RNA by using reverse transcriptase-polymerase chain reaction (RT-PCR). Only the bursa of Fabricius was found to be positive with unusually long viral persistence in the broiler group. The positive bursa samples were further investigated by using real-time PCR coupled with a TaqMan probe. The highest amounts of the virus were detected at its first appearance in the bursa: on day 14 post vaccination (PV) in the SPF chickens and on day 17 and day 21 PV in the maternally immune broiler group. The virus then gradually cleared, most likely due to the arallel appearance of the active immune response indicated by seroconversion.
Iván
Judit
J
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary. hirvi08@yahoo.com
Velhner
Maja
M
Ursu
Krisztina
K
German
Péter
P
Mató
Tamás
T
Drén
Csaba Nick
CN
Mészáros
János
J
D000818:Animals; D000936:Antigen-Antibody Complex / Q000008:administration & dosage / Q000276:immunology; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000821:virology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D007279:Injections, Subcutaneous / Q000662:veterinary; D009928:Organ Specificity; D011201:Poultry Diseases / Q000517:prevention & control*; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D019562:Viral Load / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage*
D000936:Antigen-Antibody Complex; D014765:Viral Vaccines