[Sequencing and bioinformatic analysis of NRDRiso cDNA].
This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H)-dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT-PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full-length cDNA is 1,003bp in length and was submitted to GenBank as NADP-dependent retinol dehydrogenase/reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.
Du
Jing
J
Department of Biochemistry, Shenyang Medical College, Shenyang, 110034, China. Dujing1106@sohu.com
Liu
Ge-Fei
GF
Wang
Gui-Ling
GL
Xu
Xiao-Lin
XL
Wang
Bo
B
Zhu
Li
L
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D019295:Computational Biology; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism*; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D007527:Isoenzymes; D009249:NADP; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase