Detection of lymph nodes micrometastases in Dukes' A and B colorectal cancer using anti-cytokeratin antibodies AE1/AE3.
OBJECTIVE
To detect lymph nodes micrometastases and analyze its correlation with clinicopathological parameters in Dukes' A and B colorectal cancer patients.


METHODS
One hundred and fourteen patients with colorectal cancer (Dukes' A 16; Dukes' B 98) undergoing curative operation without histological lymph nodes metastases were studied between 2001 and 2003. A total of 2,481 lymph nodes were analyzed using monoclonal cytokeratin antibody AE1/AE3 (DAKO, Carpinteria, CA) for immunohistochemistry.


RESULTS
In total, 33 (29%) patients were positive for cancer cell by immunohistochemistry. In 31 (94%) patients of them positive nodes showed single tumor cell or small groups of tumor cells; and tumor deposits measuring 0.2 and 0.37 mm in diameter in another 2 (6%) patients. Micrometastases were mainly located in the subcapsular sinus or paracortical sinus. There was no correlation between the positive lymph nodes and gender, age, tumor site, tumor size, histological type, histological grade, invasion depth, Dukes' staging and microsatellite instability (P>0.05).


CONCLUSIONS
Our findings suggest that immunohistochemical technique using monoclonal cytokeratin antibody AE1/AE3 may be a sensitive and reliable method for detecting lymph nodes micrometastases in Dukes' A and B colorectal cancer. The clinical significance of lymph nodes micrometastases is still not confirmed.
Zhou
Zhi-Wei
ZW
Department of Abdominal Surgery, Sun Yat-Sen University Cancer Center, Guangzhou 510060, Guangdong Province, China. zhouzhiw@pub.guangzhou.gd.cn
Rieger
Nick
N
Ruszkiewicz
Andrew
A
Wang
Guo-Qiang
GQ
Wan
De-Sen
DS
D000906:Antibodies / Q000032:analysis; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007633:Keratins / Q000276:immunology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D009367:Neoplasm Staging
D000906:Antibodies; D007633:Keratins