title: Phylogenetic analysis of the hexon loop 1 region of an adenovirus from psittacine birds supports the existence of a new psittacine adenovirus (PsAdV).
abstract: Adenovirus infections in psittacine birds have been well known. Most of these infections were caused by fowl adenoviruses (FAdV). In this study, liver samples showing typical histological signs of an adenovirus infection were collected from Poicephalus spp. with acute disease. A PCR amplifying the variable loop 1 region of the hexon gene was developed using primers located in two conserved pedestal regions. A PCR product of approximately 590 bp in size was amplified and sequenced. The sequence obtained grouped outside of the FAdV reference strains of the 12 serotypes as well as egg drop syndrome virus and turkey adenovirus 3 indicating that a new avian adenovirus was detected. In comparison to the FAdV reference strains, the percentage of identical nucleotides ranged between 60.3 and 67.0 and that of identical amino acids (aa) between 51.3 and 61.0. Furthermore, 37 unique aa exchanges were observed; out of these, 27 are located in the 4 hypervariable regions of loop 1, which encode the serotype-specific epitopes. The g/c content, the isoelectric point and the charge of the amplified fragment, however, are in the range as those of group I avian adenoviruses. It was proposed, therefore, to designate this new adenovirus as psittacine adenovirus (PsAdV).
authors.affiliation: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany. rudiger.raue@pfizer.com
authors.forename: R
authors.initials: R
authors.lastname: Raue
authors.forename: H
authors.initials: H
authors.lastname: Gerlach
authors.forename: H
authors.initials: H
authors.lastname: Müller
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000662:veterinary / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010311:Parrots / Q000821:virology; D010802:Phylogeny; D011563:Psittaciformes / Q000821:virology*; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral