Effect of isolated fractions of Barleria prionitis root methanolic extract on reproductive function of male rats: preliminary study.
Male rats treated with 100 mg/kg for 60 days of isolated fractions of the Barleria prionitis root methanolic extract (Fr. I and Fr. II) showed a significant reduction on spermatogenesis without affecting general body metabolism. Sperm motility as well density in cauda epididymides was reduced significantly. The fertility was decreased by 33.4% in Fr. I and 100% in Fr. II treated rats. The blood parameters were within the normal range. Total protein, glycogen and sialic acid contents of testes were reduced after the plant fractions treatment. Seminal vesicular fructose was decreased significantly after the treatment. The population of various spermatogenic cells such as primary spermatocytes, secondary spermatocytes and round spermatids were declined significantly in Fr. II treatment groups whereas in Fr. I treated animals preleptotene spermatocyte and spermatid number was decreased. There was no significant change in the number of Sertoli cells and spermatogonia in any of the treatment group.
Verma
Pramod Kumar
PK
Department of Zoology, University of Rajasthan, Jaipur 302004 (Raj), India. verma23@rediffmail.com
Sharma
Arti
A
Joshi
S C
SC
Gupta
R S
RS
Dixit
V P
VP
D027901:Acanthaceae; D000818:Animals; D003272:Contraceptive Agents, Male / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*
D003272:Contraceptive Agents, Male; D010936:Plant Extracts