[Study of IL-8 overexpression in A549 cells induced by crocidolite fibers].
OBJECTIVE
This study was to investigate the effect of crocidolite fibers on IL-8 protein and mRNA levels in A549 cells cultured supernatant and the role of tyrosine kinase inhibitor PD98059 on IL-8 expression.


METHODS
Real Time PCR and ELISA were employed to detect A549 cellular IL-8 mRNA and supernatant IL-8 protein, respectively.


RESULTS
After incubating cells with 100 microg/ml of crocidolite, IL-8 protein releasing in supernatant was significantly increased and IL-8 mRNA was also higher than that of control, the differences were statistically significant (P < 0.05), however both IL-8 protein and mRNA was abrogated by pretreating cells with tyrosine kinase inhibitor PD98059.


CONCLUSIONS
IL-8 was shown as overexpression and could be decreased by PD98059. This indicated that MAPK signal pathway is involved in the process IL-8 expression.
Wang
Xin-chao
XC
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Xu
Yu-bao
YB
Wu
Yi-ming
YM
Li
Zhuo-wei
ZW
Samet
James M
JM
Ghio
Andrew J
AJ
D017638:Asbestos, Crocidolite / Q000633:toxicity*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005419:Flavonoids; D016209:Interleukin-8; D012333:RNA, Messenger; D017638:Asbestos, Crocidolite; D011505:Protein-Tyrosine Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one