[Interleukin-6 is involved in the enhanced proliferation of HEK293 cells by the tumor specific antigen of HCA520].
OBJECTIVE
To investigate the effect of tumor specific antigen of HCA520 on the proliferation of HEK293 cells and to obtain some functional implications for HCA520.


METHODS
MTT assay was performed with HEK293 stable cell lines transfected with pcDNA3-HCA520-flag construct. Cytokines probably involved in HCA520 enhanced proliferation were screened by RT-PCR, and effect of these cytokines on the HEK293 cell proliferation was further confirmed by MTT assay.


RESULTS
HCA520 significantly promoted the proliferation of HEK293 cells, which was at least partially attributed to the up-regulation of IL-6 in HEK293 cells by HCA520.


CONCLUSIONS
HCA520 might accelerate tumorigenesis by promoting proliferation of cancerous cells.
Qian
Xiao-ping
XP
Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
Li
Guo-dong
GD
Han
Ke-jun
KJ
Chen
Wei-feng
WF
D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004622:Embryo, Mammalian; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology*; D009363:Neoplasm Proteins / Q000494:pharmacology*; D014162:Transfection
C469743:CHP1 protein, human; D002135:Calcium-Binding Proteins; D015850:Interleukin-6; D009363:Neoplasm Proteins