title: The effect of cyclo-DfKRG peptide immobilization on titanium on the adhesion and differentiation of human osteoprogenitor cells.
abstract: This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and differentiation of human osteoprogenitor (HOP) cells arising from human bone marrow were investigated. Our results seem to demonstrate that cyclo-DfKRG peptide coating with a phosphonate anchor and grafting procedure contributes to higher cell adhesion and a strong ALP and Cbfa1 mRNA expression, after 10 days of cell seeding. At the contrary, this peptide coated with a thiol anchor stimulates differentiation of HOP within 3 days of culture.
authors.affiliation: INSERM, U577, Université Victor Segalen, Bordeaux, France. stephane.pallu@bordeaux.fr
authors.forename: Stéphane
authors.initials: S
authors.lastname: Pallu
authors.forename: Chantal
authors.initials: C
authors.lastname: Bourget
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Christine
authors.initials: C
authors.lastname: Labrugère
authors.forename: Michel
authors.initials: M
authors.lastname: Dard
authors.forename: Andreas
authors.initials: A
authors.lastname: Sewing
authors.forename: Alfred
authors.initials: A
authors.lastname: Jonczyk
authors.forename: Michel
authors.initials: M
authors.lastname: Vernizeau
authors.forename: Marie
authors.initials: M
authors.lastname: Christine Durrieu
authors.forename: Joelle
authors.initials: J
authors.lastname: Amédée-Vilamitjana
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D010456:Peptides, Cyclic; C505794:cyclo-DfKRG; D014025:Titanium