title: Transplantation of human acute myeloid leukemia (AML) cells in immunodeficient mice reveals altered cell surface phenotypes and expression of human endothelial markers.
abstract: To better characterize acute myeloid leukemia (AML) development in non-obese diabetic (NOD)/severe combined immunodeficiency (SCID) mice, we transplanted samples from patients with AML or KG-1 and EOL-1 cell lines. We found 9/12 primary AML samples and both cell lines to engraft within 2-8 weeks, with 5-80% human cells in bone marrow. Compared with freshly isolated AML cells, percentages of human CD33+, CD38+, CD31+ CD13+ or CD15+ subpopulations increased after transplantation, whereas percentages of CD34+ cells decreased. Engrafted mice frequently showed expression of human endothelial cell markers. Thus, transplantation of human AML into NOD/SCID mice reveals expression of hematopoietic and endothelial differentiation markers.
authors.affiliation: Institute of Transfusion Medicine and Immune Hematology, German Red Cross Blood Center, Johann Wolfgang Goethe University, Sandhofstrasse 1, D-60528 Frankfurt, Germany. rhenschler@bsdhessen.de
authors.forename: Reinhard
authors.initials: R
authors.lastname: Henschler
authors.forename: Stephan
authors.initials: S
authors.lastname: Göttig
authors.forename: Ilse
authors.initials: I
authors.lastname: Junghahn
authors.forename: Gesine
authors.initials: G
authors.lastname: Bug
authors.forename: Erhard
authors.initials: E
authors.lastname: Seifried
authors.forename: Albrecht M
authors.initials: AM
authors.lastname: Müller
authors.forename: Iduna
authors.initials: I
authors.lastname: Fichtner
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007951:Leukemia, Myeloid / Q000145:classification / Q000276:immunology / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface; D014408:Biomarkers, Tumor