Structure-dependent and receptor-independent increase in osmotic fragility of rat erythrocytes by short-chain fatty acids.
We examined short-chain fatty acids (SCFAs) with 1 (C1) to 5 (C5) carbon atoms for osmotic fragility (OF) in isolated red blood cells (RBCs) in rats. The RBCs were used as prototypical plasma membrane model. The dense packed RBC was incubated in a phosphate-NaCl buffer solution containing each SCFA at 0 to 100 mM. The RBC suspensions were transferred into the OF test tubes containing NaCl from 0.2 to 0.9%. The hemoglobin concentration was determined and the EC50 in hemolysis was calculated. The OF in RBCs was dose-dependently increased by exposure to SCFAs, except for C1, with an increasing number of carbon atoms. Branched-chain fatty acids (isomers of C4 and C5) have a smaller effect on OF than straight-chain fatty acids (C4 and C5). The SCFA-induced increases in OF were not affected by pretreatment of RBCs with trypsin. The response of the RBC membrane to SCFAs depends on their concentration, carbon chain length and chain structure (straight or branched). The SCFAs probably disturb the lipid bilayer of the RBC membrane and result in a decrease in osmotic resistance. The plasma membrane in rat RBCs could respond to the structure of the SCFAs in detail by using the OF as an indicator.
Mineo
Hitoshi
H
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan. mineo@primarycell.com
Hara
Hiroshi
H
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape; D004305:Dose-Response Relationship, Drug; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006461:Hemolysis; D008055:Lipids / Q000737:chemistry; D008297:Male; D009995:Osmosis; D009996:Osmotic Fragility; D010710:Phosphates / Q000737:chemistry; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000737:chemistry; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000737:chemistry
D005232:Fatty Acids, Volatile; D008055:Lipids; D010710:Phosphates; D020033:Protein Isoforms; D012965:Sodium Chloride; D014357:Trypsin