title: Real-time polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease in myeloma.
abstract: The evaluation of minimal residual disease (MRD) is critical in the evaluation of treatments aimed at maximal cytoreduction in multiple myeloma (MM). Qualitative evaluation of MRD now has a 10-yr-long history, but it remains a relatively sophisticated procedure. More recently, real-time quantitative approaches have also been developed. These approaches allow a very effective monitoring of disease but introduce additional complexity and costs to the procedure. This chapter describes how we currently perform real-time polymerase chain reaction (PCR) in MM. Compared to the first description of the assay in June 2000, significant improvements have been made. Although real-time PCR is the main focus of the chapter, most of the information suitable for a proper setup of a qualitative approach is also provided.
authors.affiliation: Divisione di Ematologia, Universita' di Torino, Torno, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Compagno
authors.forename: Barbara
authors.initials: B
authors.lastname: Mantoan
authors.forename: Monica
authors.initials: M
authors.lastname: Astolfi
authors.forename: Mario
authors.initials: M
authors.lastname: Boccadoro
authors.forename: Marco
authors.initials: M
authors.lastname: Ladetto
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D007621:Karyotyping / Q000379:methods; D009101:Multiple Myeloma / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D007136:Immunoglobulins