[Application with single-stranded probes in randomized terminal linker-dependent PCR].
OBJECTIVE
Preparing single-stranded (ss) probes in place of double-stranded (ds) probes to improve the hybridization efficiency and specificity of randomized terminal linker-dependent PCR (RDPCR).


METHODS
Using asymmetric PCR and single-primer PCR to prepare ss probes of extron 7 of p53 gene in rat, then comparing the results hybridized with ss probes and ds probes.


RESULTS
Preparation of ss probes by asymmetric PCR and single-primer PCR gets success. Hybridization results showed that the ss probes could get better signals and less noise than ds probes.


CONCLUSIONS
In comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.
Rao
Chao-long
CL
School of Public Health, Sichuan University, Chengdu 610041, China.
Wu
Qing
Q
Heng
Zheng-chang
ZC
D000818:Animals; D017931:DNA Primers; D015342:DNA Probes; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes