[Analysis of CT antigen expression and humoral immunogenicity of NY-ESO-1 protein in gastric carcinoma].
OBJECTIVE
To study the expression of 11 cancer-testis (CT) genes and autologous humoral immune response to NY-ESO-1 in gastric carcinoma in China to provide some data for developing a vaccine against this cancer.


METHODS
Pathology tests were conducted in 101 gastric carcinoma samples, including tumor tissue and paired adjacent normal non-tumor tissue. Reverse transcription-polymerase chain reaction (RT-PCR) was used to test for 11 CT antigen gene expression and ELISA for autologous humoral immune response to NY-ESO-1. The antigen of NY-ESO-1 was also detected in tumor tissues by mAb E978 in IHC.


RESULTS
About 74.3% of the samples expressed at least one CT antigen, and the most frequently expressed one was MAGE-3 (41.6%). Twelve out of the 101 samples (11.9%) were found to be NY-ESO-1 mRNA positive. Simultaneously, autologous humoral immune response to NY-ESO-1 was detected in 6 of 12 NY-ESO-1 mRNA positive patients.


CONCLUSIONS
Multiple CT genes were expressed in gastric cancer samples. MAGE-3, SSX-1, SSX-4, NY-ESO-1/LAGE-1, and MAGE-1 can be chosen as candidate antigens for cancer vaccines due to their higher expression rate. The potential of a multivalent CT antigen vaccine, including NY-ESO-1 and MAGE-3 along with other CT antigens, should be evaluated in patients with gastric cancer.
Wu
Xiao-jiang
XJ
Department of Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China.
Wang
Yu
Y
Ji
Jia-fu
JF
D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D012097:Repressor Proteins / Q000235:genetics / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm; D001323:Autoantibodies; C104948:CTAG1B protein, human; C072013:MAGEA3 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012097:Repressor Proteins; C093962:synovial sarcoma X breakpoint proteins