title: Correlation between melanoma angiogenesis and the mesenchymal stem cells and endothelial progenitor cells derived from bone marrow.
abstract: Endothelial progenitor cells (EPC) reportedly differentiate into endothelial cells and participate in angiogenesis, including neovascularization at sites of neoplastic development. Recently, we reported that Flk+/CD31-/CD34- mesenchymal stem cells (MSC) possess the potential of differentiating into both endothelial and hematopoietic cells. We hypothesized that these MSC contribute to tumor angiogenesis. This concept is controversial and this study was undertaken to address this controversy. We show that progeny of human MSC as well as differentiated endothelial cells possess the ability to participate in tumor angiogenesis. When human marrow-derived MSC were injected into tail veins of severe combined immunodeficient (SCID) mice engrafted with human malignant melanoma, human cells incorporated into tumor vessels. Moreover, human-derived endothelial cells were identified in the walls of mouse tumor vessels by immunohistology. We report for the first time that similar results are obtained when mice carrying malignant melanoma are injected with differentiated human endothelial cells. Thus, we demonstrate that both differentiated endothelial cells from tissue peripheral to that of a tumor as well as progeny of human MSC have similar capacities to participate in angiogenesis.
authors.affiliation: Department of Pathology, TianJin Cancer Hospital, TianJin Medical University, TianJin 300060, China.
authors.forename: Baocun
authors.initials: B
authors.lastname: Sun
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Ni
authors.forename: Danfang
authors.initials: D
authors.lastname: Zhang
authors.forename: Yanxue
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhao
authors.forename: Mingxia
authors.initials: M
authors.lastname: Shi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D008545:Melanoma / Q000098:blood supply*; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D016513:Mice, SCID; D009389:Neovascularization, Pathologic; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous / Q000473:pathology*
chemical_list: D015703:Antigens, CD