The modulation effects of d-amphetamine and procaine on the spontaneously generated action potentials in the central neuron of snail, Achatina fulica Ferussac.
The modulation effects of d-amphetamine and procaine on the spontaneously generated action potentials were studied on the RP1 central neuron of giant African snails (Achatina fulica Ferussac). Extra-cellular application of d-amphetamine or procaine reversibly elicited bursts of potential (BoP). Prazosin, propranolol, atropine or d-tubocurarine did not alter the BoP elicited by either d-amphetamine or procaine. KT-5720 or H89 (protein kinase A inhibitors) blocked d-amphetamine-elicited BoP, whereas they did not block the procaine-elicited BoP. U73122, neomycin (phospholipase C inhibitors) blocked the procaine-elicited BoP, whereas they did not block the d-amphetamine-elicited BoP in the same neuron. These results suggest that BoP elicited by d-amphetamine or procaine were associated with protein kinase A and phospholipase C activity in the neuron.
Lin
Chia-Hsien
CH
Department of Pharmacology, College of Medicine, National Taiwan University, No.1, Sec.1, Jen-Ai Road, Taipei, Taiwan.
Tsai
Ming-Cheng
MC
D000200:Action Potentials / Q000187:drug effects*; D018674:Adrenergic Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D003913:Dextroamphetamine / Q000494:pharmacology*; D004594:Electrophysiology; D008094:Lithium / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D011343:Procaine / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012908:Snails / Q000166:cytology*; D012964:Sodium / Q000378:metabolism; D012996:Solutions; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
D018674:Adrenergic Antagonists; D018680:Cholinergic Antagonists; D047428:Protein Kinase Inhibitors; D012996:Solutions; D011343:Procaine; D008094:Lithium; D012964:Sodium; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D010738:Type C Phospholipases; D008274:Magnesium; D002118:Calcium; D003913:Dextroamphetamine