Fluorescence polarization/anisotropy approaches to study protein-ligand interactions: effects of errors and uncertainties.
Fluorescence techniques are widely used in the study of protein-ligand interactions because of their inherent sensitivity, and the fact that they can be implemented at true equilibrium conditions. Fluorescence polarization/anisotropy methodologies, in particular, are now extensively utilized in biotechnology and clinical chemistry. In this chapter, we shall discuss both theoretical and practical aspects of polarization/anisotropy methods. We shall also focus attention on considerations of errors and uncertainties in such measurements, and how these uncertainties affect the ultimate estimation of ligand-protein dissociation constants.
Jameson
David M
DM
Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
Mocz
Gabor
G
D005454:Fluorescence Polarization / Q000379:methods* / Q000706:statistics & numerical data; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012996:Solutions
D005456:Fluorescent Dyes; D008024:Ligands; D011506:Proteins; D012996:Solutions