Triacylglycerol structure and composition of hydrogenated soybean oil margarine and shortening basestocks.
The composition and structures of triacylglycerols (TAG) in a commercially prepared hydrogenated soybean oil margarine basestock [iodine value (IV) 65, 39.7% trans fatty acids] were determined by high-performance liquid chromatography (HPLC) in tandem with atmospheric pressure chemical ionization (APCI) mass spectrometry (MS). The basestock was separated by preparative HPLC into four fractions. Fractions 1 and 4, constituting approximately 8% of the total, were shown to consist of LOO, PLO, and LLS and OSS and PSS, respectively (where L = linoleic, O = oleic, S = stearic, and P = palmitic). APCI will not distinguish between O, oleic cis C18:1, and E, elaidic trans C18:1. Thus, O and E may be used interchangeably in discussion of TAG isomer structures. Fraction 2 consisted of OOO and POO. Fraction 3 consisted of OOO, POO, OOS, and POS. About 80% of the total triglycerides consisted of OOO, POO, and OOS. The trans fatty acid content of the fractions was determined, and the results showed that 92% of the total trans content was found in fractions 2 and 3. A shortening basestock (IV 81.7, 31.8% trans fatty acids) was partially characterized.
List
Gary R
GR
Food and Industrial Oil Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA. listGR@ncaur.usda.gov
Byrdwell
William C
WC
Steidley
Kevin R
KR
Adlof
Richard O
RO
Neff
William E
WE
D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006865:Hydrogenation; D008383:Margarine / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D013024:Soybean Oil / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry*
D014280:Triglycerides; D013024:Soybean Oil; D008383:Margarine