Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction.
A new method to determine the degree of deacetylation (DD) of alpha-chitin and chitosan in the range of 17-94% DD using X-ray powder diffraction (XRD) is proposed. The results were calibrated using (1)H NMR spectroscopy for chitosan and FTIR for chitin, in comparison with the potentiometric titration method. The results showed a good linear correlation between the CrI020 from XRD and the calibrated DD value. This method is found to be simple, rapid and nondestructive to the sample.
Zhang
Yongqin
Y
Department of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Xue
Changhu
C
Xue
Yong
Y
Gao
Ruichang
R
Zhang
Xiuli
X
D000107:Acetylation; D000818:Animals; D002138:Calibration; D002236:Carbohydrate Conformation; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D033362:Powder Diffraction; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction / Q000379:methods*
D002686:Chitin; D048271:Chitosan