title: ANOSVA: a statistical method for detecting splice variation from expression data.
abstract: BACKGROUND
  Many or most mammalian genes undergo alternative splicing, generating a variety of transcripts from a single gene. New information on splice variation is becoming available through technology for measuring expression levels of several exons or splice junctions per gene. We have developed a statistical method, ANalysis Of Splice VAriation (ANOSVA) to detect alternative splicing from expression data. Since ANOSVA requires no transcript information, it can be applied when the level of annotation is poor. When validated against spiked clone data, it generated no false positives and few false negatives. We demonstrated ANOSVA with data from a prototype mouse alternative splicing array, run against normal adult tissues, yielding a set of genes with evidence of tissue-specific splice variation.
  
  
  BACKGROUND
  The results are available at the supplementary information site.
  
  
  BACKGROUND
  The results are available at the supplementary information site https://bioinfo.affymetrix.com/Papers/ANOSVA/
authors.affiliation: Affymetrix, Inc. 3380 Central Expressway, Santa Clara, CA 95051, USA. cline@pasteur.fr
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Cline
authors.forename: John
authors.initials: J
authors.lastname: Blume
authors.forename: Simon
authors.initials: S
authors.lastname: Cawley
authors.forename: Tyson A
authors.initials: TA
authors.lastname: Clark
authors.forename: Jing-Shan
authors.initials: JS
authors.lastname: Hu
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Nathan
authors.initials: N
authors.lastname: Salomonis
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Alan
authors.initials: A
authors.lastname: Williams
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D005189:False Positive Reactions; D020869:Gene Expression Profiling; D051379:Mice; D015233:Models, Statistical; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D012984:Software