[The mechanism of Stat3 nuclear import].
In order to investigate the mechanism of stat3 nuclear import. positioned a characterized NLS of the SV40 large T antigen into Stat3-GFP, Dstat3-GFP respectively between the C-terminus of Stat3 and the N-terminus of GFP to create Stat3-NLS-GFP and Dstat3-NLS-GFP. With NLS-GFP as the positive control, Expression of the Stat3-NLS-GFP without IL-6 stimulation and Stat3-GFP with IL-6 stimulation resulted in a predominantly nuclear localization in 293T cell. Expression of Stat3-GFP and Dstat3-NLS-GFP without IL-6 stimulation resulted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localization in response to IL-6 is facilitated by gain of an NLS function.
Ye
Zhong-De
ZD
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Shen
Bei-Fen
BF
Song
Lun
L
D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D019913:Nuclear Localization Signals; D010957:Plasmids / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*
D019913:Nuclear Localization Signals; D050796:STAT3 Transcription Factor