Effects of dietary phytoestrogens on cardiac remodeling secondary to chronic volume overload in female rats.
Previously, we demonstrated that intact female rats fed a standard rodent diet containing soybean products exhibit essentially no adverse left ventricular (LV) remodeling in response to aortocaval fistula-induced chronic volume overload. We hypothesized that phytoestrogenic compounds in the diet contributed to the female cardioprotection. To test this hypothesis, four groups of female rats were studied: sham-operated (Sham) and fistula (Fist) rats fed a diet with [P(+)] or without [P(-)] phytoestrogens. Eight weeks postfistula, systolic and diastolic cardiac function was assessed by using a blood-perfused, isolated heart preparation. High-phytoestrogen diet had no effect on body, heart, and lung weights, or cardiac function in Sham rats. Fistula groups developed LV hypertrophy, which was not reduced by dietary phytoestrogens [1,184 +/- 229 mg Fist-P(-) and 1,079 +/- 199 mg Fist-P(+) vs. 620 +/- 47 mg for combined Sham groups, P < 0.05]. Unstressed LV volume increased in Fist-P(-) rats (428 +/- 16 vs. 300 +/- 14 microl Sham, P < 0.0001), but it was not different from Sham for Fist-P(+) animals (286 +/- 17 microl). Fist-P(-) rats developed increased ventricular compliance (5.3 +/- 0.8 vs. 2.3 +/- 0.3 microl/mmHg Sham, P < 0.01), whereas Fist-P(+) rats had no change in compliance (2.8 +/- 0.4 mul/mmHg). Intrinsic ventricular contractility was maintained in the Fist-P(+) rats, but it was reduced (P < 0.001) in the Fist-P(-) rats [systolic pressure-volume slope: 1.04 +/- 0.03, 0.60 +/- 0.06, and 0.99 +/- 0.08 mmHg/microl, for Fist-P(+), Fist-P(-), and Sham, respectively]. These data indicate that dietary phytoestrogens contribute significantly to female cardioprotection against volume overload-induced adverse ventricular remodeling and that studies evaluating gender differences in cardiovascular remodeling must consider the influence of dietary phytoestrogens.
Gardner
Jason D
JD
Department of Anatomy, Physiology, and Pharmacology, Auburn University, USA. jgardner@gwcmed.sci.edu
Brower
Gregory L
GL
Janicki
Joseph S
JS
D000818:Animals; D001018:Aortic Diseases / Q000150:complications; D001164:Arteriovenous Fistula / Q000150:complications; D002303:Cardiac Output, Low / Q000209:etiology / Q000503:physiopathology; D002316:Cardiotonic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002908:Chronic Disease; D004032:Diet; D005260:Female; D006940:Hyperemia / Q000503:physiopathology*; D017379:Hypertrophy, Left Ventricular / Q000209:etiology; D009200:Myocardial Contraction; D048789:Phytoestrogens / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D014682:Vena Cava, Inferior; D020257:Ventricular Remodeling / Q000187:drug effects*
D002316:Cardiotonic Agents; D048789:Phytoestrogens