PCR-TGGE: a method for fingerprinting the microbial flora in the small intestine of pigs.
The bacterial flora of the small intestine is still not fully understood. This is also due to the fact that a lot of these gut inhabitants aren't cultivable. PCR-TGGE (Temperature Gradient Gel Electrophoresis) is a molecular tool by which the 16S ribosomal DNA of a bacterial community can be studied for its diversity. Based on sequence differences in the variable region V6-V8, 16S ribosomal DNA fragments are separated during a TGGE run. This study shows that PCR-TGGE is a valuable tool as it gives additional information about the flora composition that could not be found with classical bacteriological techniques.
Van den Bossche
A
A
University of Ghent, Department of Animal Production, Proefhoevestraat 10, 9090 Melle, Belgium.
Van Nevel
C
C
Herman
L
L
Decuypere
J
J
De Smet
S
S
Dierick
N
N
Heyndrickx
M
M
D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary*; D007421:Intestine, Small / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*; D013696:Temperature
D004269:DNA, Bacterial