Hilbert phase microscopy for investigating fast dynamics in transparent systems.
We introduce Hilbert phase microscopy (HPM) as a novel optical technique for measuring high transverse resolution quantitative phase images associated with optically transparent objects. Because of its single-shot nature, HPM is suitable for investigating rapid phenomena that take place in transparent structures such as biological cells. The potential of this technique for studying biological systems is demonstrated with measurements of red blood cells, and its ability to quantify dynamic processes on a millisecond scale is exemplified with measurements of evaporating micrometer-sized water droplets.
Ikeda
Takahiro
T
Hamamatsu Photonics K. K., 5000 Hirakuchi, Hamakita, Shizuoka 434-8601, Japan.
Popescu
Gabriel
G
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D019047:Phantoms, Imaging