title: [Cloning and sequence analysis of human uric acid transporter gene].
abstract: OBJECTIVE
  To obtain full-length human urate transporter (hUAT) gene.
  
  
  METHODS
  Primers was designed according to the sequence of hUAT reported in Genbank. The target fragments were obtained by reverse transcriptional (RT) PCR from the mRNA extracted from human renal tubular epithelial cell lines (HK-2), followed by cloning into pEGFP-C1 plasmid. The cloned fragment was subjected to restriction mapping and sequence analysis for confirmation.
  
  
  CONCLUSIONS
  hUAT gene with correct sequence is successfully cloned from HK-2 cells. The restriction maps and sequence analysis of the selected clones were consistent with the sequence reported in Genbank.
authors.affiliation: Center of Nephrology and Key Laboratory of PLA, General Hospital of the PLA, Beijing 100853, China. redhq@163.com
authors.forename: Quan
authors.initials: Q
authors.lastname: Hong
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Xiang-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Kai
authors.initials: K
authors.lastname: Hou
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014527:Uric Acid
chemical_list: D027361:Organic Anion Transporters; C103862:urate transporter; D014527:Uric Acid