title: [The characteristics of nuclear/cytoplasmic genomes of asymmetric somatic hybrid of Daucus carota var. sativa and Swertia mussotii].
abstract: Protoplasts of Swertia mussotii irradiated by ultra-violet light (UV) 260 microW/cm(2) were fused with protoplasts of Daucus carota var. sativa using the PEG method. Analysis of 5S rDNA spacer sequences and random amplified polymorphic DNA (RAPD) showed the presence and combination of nuclear DNA from both parents. All hybrids mainly contained genomes of D. carota (receptor) RAPD bands, and a few donor RAPD bands of S. mussotii. There is no obvious relationship between the nuclear DNA composition was not affected by UV-dosage. SSR analysis of chloroplast DNA of the hybrid clones further revealed that cpDNAs of the parents were integrated and recombined in the hybrids randomly.
authors.affiliation: School of Life Sciences, Shandong Univeristy, Jinan 250100, China.
authors.forename: Zi-Dong
authors.initials: ZD
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Shao
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Cai
authors.forename: Feng-Ning
authors.initials: FN
authors.lastname: Xiang
authors.forename: Guang-Min
authors.initials: GM
authors.lastname: Xia
mesh_terms: D002678:Chimera / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D018552:Daucus carota / Q000235:genetics* / Q000528:radiation effects; D018745:Genome, Plant / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D029967:Swertia / Q000235:genetics* / Q000528:radiation effects
chemical_list: D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S