Real-time detection of virus particles and viral protein expression with two-color nanoparticle probes.
Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.
Agrawal
Amit
A
Department of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, Atlanta, Georgia 30322, USA.
Tripp
Ralph A
RA
Anderson
Larry J
LJ
Nie
Shuming
S
D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D012996:Solutions; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification; D014773:Virology / Q000379:methods
D012996:Solutions; D014764:Viral Proteins