[Isolation of thermophilic bacteria Thermus sp. YBJ-1 and cloning of amylase gene].
Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing, Tibet. The 16sr DNA sequence of YBJ-1 (1511bp in length) shares 98% identity with that of Thermus scotoductus strain ITI-252T. The full-length ORF of amylase gene of YBJ-1 (amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length, coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus, 96% with maltogenic amylse of Thermus. sp IM6501, and 81% with neopullulanase of Bacillus sterothermophlus.
Xiong
Peng-Jun
PJ
The Key Laboratory of Marine Biotechnology, The Third Institute of Oceanography, SOA, Xiamen 361005, China.
Wen
Jian-Jun
JJ
D000681:Amylases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D016366:Open Reading Frames / Q000235:genetics; D013824:Thermus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D003470:Culture Media; D000681:Amylases