Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota.
The pattern of expression of a carrot dhfr-ts gene was evaluated in different plant organs, in somatic embryos, and in hypocotyl explants induced to dedifferentiate in vitro by the addition of the synthetic auxin 2,4 dichorophenoxyacetic acid. The promoter of this gene was also placed upstream of a uidA (GUS) reporter gene and, using biolistic and protoplasts transient expression assays, was shown to drive a particularly high level of expression in actively growing suspension cells. The results from these expression analyses combined with the presence of putative cell cycle-related cis-acting elements in the dhfr-ts promoter, strongly point to a cell division-dependent expression of this gene.
Albani
D
D
Dipartimento di Botanica ed Ecologia vegetale, Università di Sassari, Italy.
Giorgetti
L
L
Pitto
L
L
Luo
M
M
Cantoni
R M
RM
Erra Pujada
M
M
Rotino
G L
GL
Cella
R
R
D002460:Cell Line; D049109:Cell Proliferation; D018552:Daucus carota / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D013940:Thymidylate Synthase / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase