title: Genetic analysis of dry matter and nitrogen accumulation and protein composition in wheat kernels.
abstract: The maximum rate and duration for grain dry matter (DM) and nitrogen (N) accumulation were evaluated in 194 recombinant inbred lines (RILs) from a cross between the two French wheat cultivars Récital and Renan. These cultivars were previously identified as having contrasting kinetics of grain DM and N accumulation. Grain protein composition was analysed by capillary electrophoresis (CE), which enabled quantification of the different storage protein fractions (alphabetagamma-gliadins, omega-gliadins, LMW glutenins, HMW glutenins, and each of their subunits). Correlation analyses revealed that DM and N accumulation rates were closely correlated and repeatable over several years, which was not the case for DM and N accumulation durations, and that protein composition was primarily influenced by the N accumulation rate. This was particularly true for the LMW-glutenins and the alphabetagamma-gliadins, the most abundant protein fractions. A genetic map of 254 molecular markers covering nearly 80% of the wheat genome was used for quantitative trait loci (QTL) analysis. A total of seven QTLs were found. Five QTLs were significantly associated with the kinetics of DM and N accumulation, and two of them also influenced protein composition. Two QTLs affected only the protein composition. One major QTL explained more than 70% of the total variation in HMW-GS Glu1B-x content.
authors.affiliation: UMR Amélioration et Santé des Plantes, INRA-Université Blaise Pascal, 234 Avenue du Brézet, 63100 Clermont-Ferrand, France. charmet@clermont.inra.fr
authors.forename: G
authors.initials: G
authors.lastname: Charmet
authors.forename: N
authors.initials: N
authors.lastname: Robert
authors.forename: G
authors.initials: G
authors.lastname: Branlard
authors.forename: L
authors.initials: L
authors.lastname: Linossier
authors.forename: P
authors.initials: P
authors.lastname: Martre
authors.forename: E
authors.initials: E
authors.lastname: Triboï
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D009584:Nitrogen / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D040641:Quantitative Trait Loci; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D014908:Triticum / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D009584:Nitrogen