title: [c-myc gene silencing in K562 cells with RNA interference].
abstract: OBJECTIVE
  To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells.
  
  
  METHODS
  siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting.
  
  
  RESULTS
  Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate.
  
  
  CONCLUSIONS
  RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.
authors.affiliation: Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. yuefeng@fimmu.com
authors.forename: Feng
authors.initials: F
authors.lastname: Yue
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Song
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D020868:Gene Silencing; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering