title: DNA adduct formation of benzo[a]pyrene in white blood cells of workers exposed to polycyclic aromatic hydrocarbons.
abstract: The major DNA adducts of anti-benzo[a]pyrene diolepoxide (BPDE) were determined by high performance liquid chromatography with fluorescence detection (HPLC-FLD) in white blood cells (WBC) of workers exposed to benzo[a]pyrene (B[a]P). In addition, ambient concentrations of B[a]P at the workplace were determined by personal air sampling. Workers in a refractory setting were examined before (n=26) and 3 months after (n = 33) changing the production material (binding pitch). Furthermore, 9 coke oven workers were examined. The change in the production process in the refractory setting led to a decrease in the median of ambient B[a]P concentrations (0.14 to <0.07 microg/m3). The median of BPDE-DNA adduct levels in WBC also decreased from 0.9 adducts/10(8) nucleotides before changing the production material to <0.5 adducts/10(8) nucleotides 3 months afterwards. The B[a]P concentrations at the workplace for the coke oven workers were found to be significantly higher than in the refractory setting. However, BPDE-DNA adduct concentrations in coke oven workers and refractory setting workers showed no significant difference, which was probably due to the low number of studied subjects in the coke-oven setting. No significant differences could be observed for BPDE-DNA adduct levels between current smokers (n=21) and non-smokers (n=14; p = 0.93) from both plants. In addition, no correlation between B[a]P concentrations in the air and DNA adduct levels in refractory workers and in coke oven workers could be found (r = -0.03, p = 0.87). Because of the missing correlation between personal air sampling and BPDE-DNA adduct levels in WBC, the results may indicate that their formation is either influenced by other routes of exposure to B[a]P (e.g., skin absorption, dietary habits) or interindividual differences in their formation and repair.
authors.affiliation: Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, Institut für Arbeitsmedizin der Ruhr-Universität Bochum, Bürkle-de-la-Camp-Platz 1, 44789 Bochum, Germany. mensing@bgfa.ruhr-uni-bochum.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Mensing
authors.forename: Boleslaw
authors.initials: B
authors.lastname: Marczynski
authors.forename: Beate
authors.initials: B
authors.lastname: Engelhardt
authors.forename: Michael
authors.initials: M
authors.lastname: Wilhelm
authors.forename: Ralf
authors.initials: R
authors.lastname: Preuss
authors.forename: Martin
authors.initials: M
authors.lastname: Kappler
authors.forename: Jürgen
authors.initials: J
authors.lastname: Angerer
authors.forename: Heiko U
authors.initials: HU
authors.lastname: Kafferlein
authors.forename: Michael
authors.initials: M
authors.lastname: Scherenberg
authors.forename: Albrecht
authors.initials: A
authors.lastname: Seidel
authors.forename: Thomas
authors.initials: T
authors.lastname: Brüning
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003077:Coke; D018736:DNA Adducts / Q000097:blood / Q000187:drug effects*; D005858:Germany; D006801:Humans; D016273:Occupational Exposure
chemical_list: D003077:Coke; D018736:DNA Adducts; D001564:Benzo(a)pyrene; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide