Heme oxygenase-1 inhibits cigarette smoke-induced increase in the tracheal mucosal permeability in guinea pigs in vivo.
OBJECTIVE
To examine whether heme oxygenase (HO)-1 inhibits cigarette smoke (CS)-induced increase in the airway mucosal permeability.


METHODS
Mucosal permeability was quantified by monitoring the rate of appearance in the circulation of horseradish peroxidase that had been instilled into the isolated tracheal segment in guinea pigs in vivo, after exposure to CS or room air.


RESULTS
Exposure to 10 puffs of CS did not increase the tracheal mucosal permeability but did increase the permeability after pretreatment with zinc protoporphyrin, a competitive inhibitor of HO-1. Moreover, pretreatment with hemin, a potent inducer of HO-1, inhibited the increase in the permeability of the tracheal mucosa induced by 20 puffs of CS exposure.


CONCLUSIONS
It is concluded that HO-1 has an important role in suppressing the increase in the mucosal permeability induced by CS in guinea pig trachea.
Tagawa
A
A
Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama-City 236-0004, Japan.
Kaneko
T
T
Shinohara
T
T
Ueda
A
A
Sato
T
T
Ishigatsubo
Y
Y
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology*; D051547:Heme Oxygenase-1; D006427:Hemin / Q000378:metabolism; D007249:Inflammation; D008297:Male; D009092:Mucous Membrane / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D010539:Permeability; D011524:Protoporphyrins / Q000378:metabolism; D012906:Smoke; D012907:Smoking; D013997:Time Factors; D014026:Nicotiana; D014028:Tobacco Smoke Pollution; D014132:Trachea / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation; D015032:Zinc / Q000378:metabolism
D004791:Enzyme Inhibitors; D016877:Oxidants; D011524:Protoporphyrins; D012906:Smoke; D014028:Tobacco Smoke Pollution; C017803:zinc protoporphyrin; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D015032:Zinc