title: Histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein.
abstract: Histone acetyltransferase (HAT) activities of proteins such as p300, CBP, and P/CAF play important roles in activation of gene expression. We now show that the HAT activity of p300 can also be required for down-regulation of transcription by a DNA binding repressor protein. Promyelocytic leukemia zinc finger (PLZF), originally identified as a fusion with retinoic acid receptor alpha in rare cases of all-trans-retinoic acid-resistant acute promyelocytic leukemia, is a transcriptional repressor that recruits histone deacetylase-containing corepressor complexes to specific DNA binding sites. PLZF associates with p300 in vivo, and its ability to repress transcription is specifically dependent on HAT activity of p300 and acetylation of lysines in its C-terminal C2-H2 zinc finger motif. An acetylation site mutant of PLZF does not repress transcription and is functionally deficient in a colony suppression assay despite retaining its abilities to interact with corepressor/histone deacetylase complexes. This is due to the fact that acetylation of PLZF activates its ability to bind specific DNA sequences both in vitro and in vivo. Taken together, our results indicate that a histone deacetylase-dependent transcriptional repressor can be positively regulated through acetylation and point to an unexpected role of a coactivator protein in transcriptional repression.
authors.affiliation: Section of Haemato-Oncology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom.
authors.forename: Fabien
authors.initials: F
authors.lastname: Guidez
authors.forename: Louise
authors.initials: L
authors.lastname: Howell
authors.forename: Mark
authors.initials: M
authors.lastname: Isalan
authors.forename: Marek
authors.initials: M
authors.lastname: Cebrat
authors.forename: Rhoda M
authors.initials: RM
authors.lastname: Alani
authors.forename: Sarah
authors.initials: S
authors.lastname: Ivins
authors.forename: Itsaso
authors.initials: I
authors.lastname: Hormaeche
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: McConnell
authors.forename: Sarah
authors.initials: S
authors.lastname: Pierce
authors.forename: Philip A
authors.initials: PA
authors.lastname: Cole
authors.forename: Jonathan
authors.initials: J
authors.lastname: Licht
authors.forename: Arthur
authors.initials: A
authors.lastname: Zelent
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C092037:ZBTB16 protein, human; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D016650:Fluorescein-5-isothiocyanate