title: 1,25-Dihydroxyvitamin D3 suppresses gene expression of eukaryotic translation initiation factor 2 in human promyelocytic leukemia HL-60 cells.
abstract: The physiologically active metabolite of vitamin D(3), 1alpha,25-dihydroxyvitamin D(3) (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.
authors.affiliation: Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21st Century, University of Shizuoka, Yada, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yu
authors.initials: Y
authors.lastname: Koyama
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Tsushima
authors.forename: Kouichi
authors.initials: K
authors.lastname: Abe
authors.forename: Sumio
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuruto-Niwa
authors.forename: Ryushi
authors.initials: R
authors.lastname: Nozawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Isemura
mesh_terms: D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D015852:Eukaryotic Initiation Factor-2 / Q000096:biosynthesis / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015852:Eukaryotic Initiation Factor-2; D009363:Neoplasm Proteins; D002117:Calcitriol