title: [Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos].
abstract: By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduated School of Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yu
authors.forename: Wen-Yong
authors.initials: WY
authors.lastname: Li
authors.forename: Yu-Ge
authors.initials: YG
authors.lastname: Wang
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Yang
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Liu
authors.forename: Miao
authors.initials: M
authors.lastname: Du
authors.forename: Qing-Xuan
authors.initials: QX
authors.lastname: Chen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism* / Q000502:physiology; D015152:Blotting, Northern; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011817:Rabbits