First partial characterisation of small ruminant lentiviruses from Greece.
Small ruminant lentivirus (SRLV) infections are widespread in Greece, but SRLVs have never been isolated and characterized. In this study, we present the sequence of a 574-nucleotide (191-amino acid) region of the gag gene of SRLV strains from four sheep and one goat from a single geographic area of Greece. All five sequences appeared to be closely related at both nucleotide (2.1-14.2% variation) and deduced amino acid (1.6-4.2% variation) level. Greek SRLV strains were closer to ovine prototypic strains (average divergence 16.8%) than to the caprine strain CAEV-Co (21% divergence). By amino acid composition, the Greek SRLVs were on the average more than twice as distant from CAEV-Co as from other ovine strains. Phylogenetic analysis suggested that Greek strains segregate into a unique group, separate from, but related to, other ovine prototype sequences.
Angelopoulou
K
K
Laboratory of Biochemistry and Toxicology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Karanikolaou
K
K
Papanastasopoulou
M
M
Koumpati-Artopiou
M
M
Vlemmas
I
I
Papadopoulos
O
O
Koptopoulos
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D006115:Greece; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D017972:Lentiviruses, Ovine-Caprine / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*
D004279:DNA, Viral