[Anti-tumor effect of anti-dsDNA autoantibodies].
OBJECTIVE
To investigate effects of anti-dsDNA autoantibodies on growth of tumor in vitro and in vivo.


METHODS
BALB/c mice were inoculated with inactivated tumor cells and challenged s.c. with SP 2/0 and Wehi 164 tumor cells four weeks after the last inoculation. The naïve mice were inoculated with SP 2/0 tumor cells immediately after incubating with sera derived from the immunized mice at week 6. Then the tumor size was examined. In vitro, the cytotoxicity of anti-dsDNA autoantibodies to tumor cells was analysed. Furthermore, apoptosis of SP 2/0 and Wehi 164 tumor cells induced by anti-dsDNA autoantibodies was examined by FACS.


RESULTS
In vivo study showed that the growth of SP 2/0 and Wehi 164 tumors were inhibited in mice with anti-dsDNA autoantibodies, but not in mice lack of anti-dsDNA autoantibodies. In vitro, apoptosis of SP 2/0 and Wehi 164 tumor cells was induced when the tumor cells were incubated with the sera containing anti-dsDNA autoantibodies. Statistical analysis showed that the ability of anti-dsDNA autoantibodies to induce apoptosis of SP 2/0 and Wehi 164 tumor cells was significantly correlated with affinity (r = 0.990, P < 0.01 and r = 0.901, P < 0.05).


CONCLUSIONS
Anti-dsDNA autoantibodies have inhibitory effect on tumor cells via inducing apoptosis.
Lü
Shun
S
Center for Gene Immunization and Vaccine Research, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Zhang
Jin-ping
JP
Wu
Hou-sheng
HS
Zheng
Xiu-juan
XJ
Chu
Yi-wei
YW
Xiong
Si-dong
SD
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology*; D017209:Apoptosis; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology*; D045744:Cell Line, Tumor; D004247:DNA / Q000276:immunology*; D005354:Fibrosarcoma / Q000473:pathology / Q000517:prevention & control; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009101:Multiple Myeloma / Q000473:pathology / Q000517:prevention & control*; D009368:Neoplasm Transplantation
D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D007106:Immune Sera; D004247:DNA