Immunoblot analysis of specific antigen bands predictable for Dirofilaria immitis infection in cats.
Serial sera from four mongrel cats experimentally inoculated with infectious larvae of Dirofilaria immitis were analyzed by immunoblot patterns against a phosphate buffered saline-extract of D. immitis. Antigen-specific protein bands detected indicate that the low molecular weight bands of 36, 32, 22, 19 and 14 kDa, are predictable for positive adult worm infection, suggesting diagnostic usefulness for adult D. immitis infection in cats.
Hayasaki
Mineo
M
Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. hayasaki@yamaguchi-u.ac.jp
Mori
Naoko
N
Hongo
Haruka
H
Katsuya
Akiyo
A
Song
Kun-Ho
KH
Une
Satoshi
S
Ikeda
Tadao
T
Ooi
Hong-Kean
HK
Uchida
Akihiko
A
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000097:blood*; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D002415:Cats; D004183:Dirofilaria immitis / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D005260:Female; D008297:Male
D000909:Antibodies, Helminth; D000947:Antigens, Helminth