title: Matrix molecule mRNA levels in the bursa and rotator cuff of patients with full-thickness rotator cuff tears.
abstract: OBJECTIVE
  The purpose of this study was to evaluate at the mRNA level a subset of extracellular matrix molecules relevant during healing and remodeling of rotator cuff tears.
  
  
  METHODS
  Controlled laboratory study.
  
  
  METHODS
  Bursal and rotator cuff tissue from the margin of the rotator cuff tear were harvested from 10 patients (mean age, 57.5 +/- 7.3 years) undergoing surgical repair of full-thickness rotator cuff tears. There were six male and four female patients with a mean duration of symptoms of 14.6 months (range, 2 to 60 months). The mean tear size was 4.4 cm. In addition, tissue was obtained from 6 cadaveric specimens with no gross evidence of rotator cuff tearing. Reverse transcription polymerase chain reaction (RT-PCR) was performed for type I, II, and III collagen, biglycan, decorin, and aggrecan, and normalized to the housekeeping gene GAPDH.
  
  
  RESULTS
  RT-PCR showed that both the bursa and rotator cuff margin had increased mRNA levels for type I and type III collagen in patients with full-thickness rotator cuff tears when compared with normal cadaveric controls. In addition, there was a significant decrease in decorin mRNA levels and an increase in aggrecan mRNA levels in the rotator cuff margin of torn rotator cuff tendons when compared with normal cadaveric controls.
  
  
  CONCLUSIONS
  These results suggest that both the bursa and rotator cuff margin of patients with rotator cuff tears are actively remodeling after injury and that both tissues may potentially contribute to the healing process following repair.
  
  
  CONCLUSIONS
  These findings may help clinicians determine what aspects of the repair process can be manipulated to affect optimal ruptured tendon repair.
authors.affiliation: McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Calgary, Alberta, Canada. ikylo@ucalgary.ca
authors.forename: Ian K Y
authors.initials: IK
authors.lastname: Lo
authors.forename: Richard
authors.initials: R
authors.lastname: Boorman
authors.forename: Linda
authors.initials: L
authors.lastname: Marchuk
authors.forename: Robert
authors.initials: R
authors.lastname: Hollinshead
authors.forename: David A
authors.initials: DA
authors.lastname: Hart
authors.forename: Cyril B
authors.initials: CB
authors.lastname: Frank
mesh_terms: D002061:Bursa, Synovial / Q000293:injuries* / Q000378:metabolism; D002062:Bursitis / Q000235:genetics; D002102:Cadaver; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017006:Rotator Cuff / Q000378:metabolism*; D000070636:Rotator Cuff Injuries
chemical_list: D011509:Proteoglycans; D012333:RNA, Messenger