[Expression and secretion of human tumor necrosis factor gene transfected on human embryo myoblasts].
OBJECTIVE
To observe human tumor necrosis factor-alpha (hTNF-alpha) expression and secreting level of human embryo myoblasts transfected by hTNF-alpha gene.


METHODS
Human embryo myoblasts were transfected with shuttle plasmid pSV23SHTNF containing hTNF-alpha gene by cationic liposomes DOSPER. The control group was only given equivalent liposomes except plasmid. After culturing for 24, 48, 72 and 96 hours, hTNF-alpha expression level of human embryo myoblasts was observed with immunocytochemistry staining, and hTNF-alpha secreting of human embryo myoblasts was analyzed by ELISA.


RESULTS
After transfected by hTNF-alpha gene for 24, 48, 72 and 96 hours, the human embryo myoblasts displayed significant secretion of hTNF-alpha in the cultural supernatant (P < 0.05), and overexpression in cytoplasma and cell membrane.


CONCLUSIONS
Transfection of hTNF-alpha gene to human myoblasts made myoblasts secrete high concentration of hTNF-alpha, implying it is feasible that transfecting muscle cells surrounding tongue carcinoma lesion with hTNF-alpha gene can prevent tongue carcinoma from intruding into deeper muscle tissue.
Gao
Zhen-Nan
ZN
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Cao
Jia-Rang
JR
Tian
Wei-Dong
WD
Li
Sheng-Wei
SW
Liu
Lei
L
Fu
Chun-Hua
CH
D000818:Animals; D015316:Genetic Therapy; D006801:Humans; D032446:Myoblasts; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D010957:Plasmids; D014062:Tongue Neoplasms / Q000628:therapy*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha