All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion.
The major secreted virulence factor of Helicobacter pylori, the vacuolating cytotoxin VacA, is known to insert into eukaryotic membranes and has been observed in association with the surface of H. pylori cells that are actively producing it. Here, it is demonstrated that VacA is capable of interacting with the surface of H. pylori and Escherichia coli after secretion. It is shown that this interaction is resistant to disruption of electrostatic and hydrophobic forces, and that it appears to occur despite truncation of LPS and the removal of trypsin-accessible surface proteins. Adsorption to bacterial cell surfaces was independent of the VacA subtype, suggesting that it is not mediated through recognition of a known receptor by the VacA p58 subunit. Similarly, adsorption to bacterial cell surfaces is unlikely to be instigated by the extreme N-terminus of VacA, since a hydrophilic extension at this location that is known to disrupt VacA-induced vacuolation did not interfere with adsorption to H. pylori cells.
Fitchen
Nicola
N
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
Letley
Darren P
DP
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
O'Shea
Paul
P
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
Atherton
John C
JC
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
Williams
Paul
P
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
Hardie
Kim R
KR
Institute of Infection, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RH, UK 2Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK 3School of Biomedical Sciences, Queen's Medical School, Nottingham NG7 2UH, UK.
D000327:Adsorption; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D003603:Cytotoxins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity; D011817:Rabbits; D014617:Vacuoles; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
D001426:Bacterial Proteins; D003603:Cytotoxins; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors