title: Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus.
abstract: Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field.
authors.affiliation: Laboratory of Virology, Wageningen University, The Netherlands.
authors.forename: Marcel
authors.initials: M
authors.lastname: Prins
authors.affiliation: Laboratory of Virology, Wageningen University, The Netherlands.
authors.forename: Dick
authors.initials: D
authors.lastname: Lohuis
authors.affiliation: Laboratory of Molecular Recognition and Antibody Technology, Wageningen University, The Netherlands.
authors.forename: Arjen
authors.initials: A
authors.lastname: Schots
authors.affiliation: Laboratory of Virology, Wageningen University, The Netherlands.
authors.forename: Rob
authors.initials: R
authors.lastname: Goldbach
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D019151:Peptide Library; D010935:Plant Diseases / Q000821:virology; D030821:Plants, Genetically Modified / Q000276:immunology*; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017865:Tospovirus / Q000276:immunology / Q000472:pathogenicity*
chemical_list: D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D019151:Peptide Library