title: Non-invasive in situ simultaneous measurement of multi-parameter mechanical properties of red blood cell membrane.
abstract: The purpose of this study was to develop a new dynamic image analyzing technique that will give us the ability to measure the viscoelastic parameters of individual living red blood cells non-invasively, in situ and in real time. With this technique, the bending modulus KC, the shear elasticity mu and their ratio were measured under different temperatures, oxygen partial pressures and osmotic pressures. The results not only show the effects of external conditions on mechanical properties of cell membranes including deformability, flexibility, adhesive ability and plasticity, but also demonstrate that the technique can be used to measure cell membrane parameters continuously under several physiological and pathological conditions.
authors.affiliation: Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, China. lsslij@gzsums.edu.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yao-Xiong
authors.initials: YX
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Ji
authors.forename: Mei
authors.initials: M
authors.lastname: Tu
authors.forename: Xuan
authors.initials: X
authors.lastname: Mao
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Chen
authors.forename: Guang-Wei
authors.initials: GW
authors.lastname: Chen
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques / Q000295:instrumentation; D004548:Elasticity; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008858:Microscopy, Phase-Contrast; D009997:Osmotic Pressure; D010100:Oxygen; D010313:Partial Pressure; D013696:Temperature
chemical_list: D010100:Oxygen