title: [Application with single-stranded probes in randomized terminal linker-dependent PCR].
abstract: OBJECTIVE
  Preparing single-stranded (ss) probes in place of double-stranded (ds) probes to improve the hybridization efficiency and specificity of randomized terminal linker-dependent PCR (RDPCR).
  
  
  METHODS
  Using asymmetric PCR and single-primer PCR to prepare ss probes of extron 7 of p53 gene in rat, then comparing the results hybridized with ss probes and ds probes.
  
  
  RESULTS
  Preparation of ss probes by asymmetric PCR and single-primer PCR gets success. Hybridization results showed that the ss probes could get better signals and less noise than ds probes.
  
  
  CONCLUSIONS
  In comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.
authors.affiliation: School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Chao-long
authors.initials: CL
authors.lastname: Rao
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Zheng-chang
authors.initials: ZC
authors.lastname: Heng
mesh_terms: D000818:Animals; D017931:DNA Primers; D015342:DNA Probes; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015342:DNA Probes