Parallel detection and quantification using nine immunoassays in a protein microarray for drug from serum samples.
A protein microarray system for detection and quantification of nine prohibited drugs in serum is described. Chemically modified slides were chosen as the microarray substrates because of their suitable for drug-BSA printing. The developed protein microarray was able to preserve the biological function of the haptens, when immobilized on the microarray surface and demonstrated binding with their corresponding antibodies. The microarray could also be used for quantitative analysis when mouse IgG was chosen as an internal control for data processing. There was no qualitative difference between the results obtained using the protein microarray and ELISA. The protein microarray technology should be applicable to performing, simultaneously, large scale screening tests for many different analytes in serum.
Du
Hongwu
H
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Yang
Weiping
W
Xing
Wanli
W
Su
Yao
Y
Cheng
Jing
J
D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D013287:Illicit Drugs / Q000097:blood*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D015813:Substance Abuse Detection / Q000295:instrumentation* / Q000379:methods
D013287:Illicit Drugs