title: High genetic diversity of human immunodeficiency virus type 1 in Angola.
abstract: To investigate which HIV-1 genetic forms are circulating in Angola, we have determined the gag and/or env genotypes of 48 isolates from patients living in Cabinda and Luanda provinces. The following subtypes were identified: A1 (18 samples, 38%), C (7, 15%), H (5, 10%), J (3, 6%), G (2, 4%), A2 (2, 4%), F1 (1, 2%), and D (1, 2%). The env gene fragment was untypable in one sample. Discordant subtype classifications in the gag and env genes were found in eight (17%) samples. There were six different recombination patterns (gag/env): A1/H (3, 6%), A1/G (1, 2%), C/A2 (1, 2%), F1/B (1, 2%), G/B (1, 2%), and G/H (1, 2%). The A1/H recombinant may represent a new circulating recombinant form. The marked genetic heterogeneity of HIV-1 in Angola has important implications for vaccine development.
authors.affiliation: Instituto Superior de Ciências da Saúde Sul, Monte de Caparica, 2829-511 Caparica, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Bártolo
authors.forename: Marta
authors.initials: M
authors.lastname: Epalanga
authors.forename: José
authors.initials: J
authors.lastname: Bartolomeu
authors.forename: Marlene
authors.initials: M
authors.lastname: Fonseca
authors.forename: Ana
authors.initials: A
authors.lastname: Mendes
authors.forename: António
authors.initials: A
authors.lastname: Gama
authors.forename: Nuno
authors.initials: N
authors.lastname: Taveira
mesh_terms: D000810:Angola; D015752:Genes, env; D015750:Genes, gag; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins