[Leukemic bone marrow stromal cells in vitro protect leukemic cell line Jurkat cells from daunorubicin-induced apoptosis].
OBJECTIVE
Tumor microenvironment affects tumor cells growth. Bone marrow microenvironment may protect leukemic cells from drug-induced damages, but the mechanism is unclear. This study was to explore the protection of bone marrow stromal cells (BMSCs) on leukemic cells against chemotherapy and its mechanism.


METHODS
Normal and leukemic BMSCs were isolated using Percoll, and cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. After treatment of 0.5 micromol/L of daunorubicin (DNR), apoptosis and cell cycle distribution of Jurkat cells were analyzed by flow cytometry.


RESULTS
When treated with DNR for 24 h, apoptosis rate of normal BMSCs-cocultured Jurkat cells was significantly lower than that of Jurkat cells without coculture [(8.39+/-4.08)% vs. (16.02+/-1.00)%, P < 0.05], and apoptosis rate of leukemic BMSCs-cocultured Jurkat cells was significantly lower than that of normal BMSCs-cocultured Jurkat cells [(5.73+/-1.78)% vs. (8.39+/-4.08)%, P < 0.05]; G(0)/G(1) phase percentage of BMSCs-cocultured Jurkat cells was significantly higher than that of Jurkat cells without coculture (P < 0.05), but the difference between Jurkat cells cocultured with normal and leukemic BMSCs was not significant (P > 0.05).


CONCLUSIONS
Leukemic BMSCs may inhibit DNR-induced apoptosis in leukemic cells partly through G(0)/G(1) phase arrest.
Li
Zhong-Jun
ZJ
Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P. R. China.
Teng
Ben-Xiu
BX
Chen
Xing-Hua
XH
Kong
Pei-Yan
PY
Wang
Ji-Gang
JG
Peng
Xian-Gui
XG
Yang
Zhen-Zhou
ZZ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D018920:Coculture Techniques; D003630:Daunorubicin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007399:Interphase / Q000187:drug effects; D019169:Jurkat Cells; D007938:Leukemia / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D000903:Antibiotics, Antineoplastic; D003630:Daunorubicin