Congener-specific tissue distribution of aroclor 1254 and a highly chlorinated environmental PCB mixture in rats.
Polychlorinated biphenyl (PCB) mixtures were synthesized and marketed in Eastern European countries, but little is known abouttheir composition, distribution, ortoxicity. PCB-contaminated soil from the former production site of the Polish PCB mixture Chlorofen was collected, and the PCBs were extracted. An in vivo study was performed to investigate the PCB tissue distribution and biochemical effects of this soil extract in immature male Sprague-Dawley rats. Rats were administered 0.05 mmol/kg soil-extracted PCBs or Aroclor 1254 and sacrificed 7 days later, and congener-specific PCB profiles in selected tissues were determined. Distribution of SigmaPCBs (sum of 120 congeners) in tissues was primarily a function of lipid content of the tissues, except for the spleen, which retained more PCB than other tissues. Multivariate analysis of the PCB congener data showed that (a) congener profiles in tissues had changed, as compared to the parent mixture; (b) disposition and redistribution of individual congeners in vivo differed between both mixtures; and (c) more highly chlorinated congeners were retained in the spleens of both treatment groups. Differences in the induction of cytochrome P-450 1A and 2B subfamilies reflected the homologue composition of the respective mixtures and predict a different toxicity profile for Chlorofen than for Aroclor 1254.
Kania-Korwel
Izabela
I
Department of Occupational and Environmental Health, College of Public Health, University of lowa, Iowa City, Iowa 52242, USA.
Hornbuckle
Keri C
KC
Peck
Aaron
A
Ludewig
Gabriele
G
Robertson
Larry W
LW
Sulkowski
Wieslaw W
WW
Espandiari
Parvaneh
P
Gairola
C Gary
CG
Lehmler
Hans-Joachim
HJ
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001921:Brain / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism; D006256:Hazardous Waste; D007668:Kidney / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009206:Myocardium / Q000737:chemistry; D011044:Poland; D011078:Polychlorinated Biphenyls / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry; D012989:Soil Pollutants / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry; D014018:Tissue Distribution
D006256:Hazardous Waste; D008055:Lipids; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1