Menadione protects gastric mucosa against ethanol-induced ulcers.
Previous studies have shown a definite role of mitogen activated protein kinase (MAPK) and epidermal growth factors (EGF) in the maintenance and repair of gastric mucosa. The aim of this study is to investigate the effect of menadione, an activator of MAPK pathway, on gastric acid secretion and experimentally induced gastric ulcer in rats. Acid secretion studies were undertaken using pylorus-ligated rats pretreated with menadione (5 - 45 mg/kg, i.p.). The effect of orally administered menadione on ethanol-induced gastric ulcers was also examined. The level of gastric wall mucus, non-protein sulfhydryls (NP-SH) and myeloperoxidase (MPO) was measured in the glandular stomach of rats following ethanol-induced gastric lesions. There was a significant inhibition of gastric acid secretion in the menadione treated rats. Pretreatment of rats with menadione significantly protected gastric mucosa against ethanol-induced gastric lesion. A significant attenuation of ethanol-induced reduction of gastric wall mucus, depletion of NP-SH and increase in gastric MPO activity was also observed in menadione treated rats. In conclusion, this study clearly showed acid antisecretory and antiulcer activity of menadione. Further studies are warranted to determine the mechanism of antiacid and gastroprotective effect of menadione.
Tariq
Mohammad
M
Research Center and Department of Pathology, Armed Forces Hospital, P. O. Box 7897, (W-912) Riyadh 11159, Saudi Arabia. yasom70@hotmail.com
Al Moutaery
Ahmed
A
D000284:Administration, Oral; D000818:Animals; D000933:Antifibrinolytic Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005260:Female; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008026:Ligation; D008297:Male; D009093:Mucus / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011708:Pylorus / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology*
D000933:Antifibrinolytic Agents; D013438:Sulfhydryl Compounds; D000431:Ethanol; D024483:Vitamin K 3; D009195:Peroxidase