[Effect of granulocyte-macrophage colony stimulating factor on the repair of vessel intima damaged by balloon].
OBJECTIVE
To observe the effect of granulocyte-macrophage colony stimulating factor (GM-CSF) on the repair of damaged iliac arteries.


METHODS
Twenty-four male New Zealand White rabbits were randomized to two equal groups: GM-CSF group receiving subcutaneous injection of GM-CSF (10 microg.kg(-1).d(-1)), and control group receiving subcutaneous injection of normal saline. Seven days later all rabbits underwent insertion and expansion of balloon into the right iliac arteries so as to cause damage. Four weeks after the operation the operated iliac arteries of the GM-CSF group and the left iliac arteries of the control group were collected to undergo pathological examination. The repair and hyperplasia of intima were observed by microscopy and the indices of restenosis were evaluated by computerized planimetry.


RESULTS
Histological examination showed that hyperplasia of neogenetic intima was lighter, numbers of vascular smooth muscle cells and fibrous tissue were less, degree of lumen stenosis was slighter, and the endothelium was more integrated in the GM-CSF group in comparison with the control group. Morphometry showed that the lumen area of the GM-CSF group was 1.27 mm(2) +/- 0.31 mm(2), significantly larger than that of the control group (0.92 mm(2) +/- 0.24 mm(2), P < 0.05). The area of neogenetic intima of the GM-CSF group was 0.85 mm(2) +/- 0.34 mm(2), significant smaller than that of the control group (1.18 +/- 0.38, P < 0.05), and the percentage of intimal hyperplasia of the GM-CSF group was 40% +/- 7%; significantly lower than that of the control group (55% +/- 6%, P < 0.05).


CONCLUSIONS
GM-CSF promotes the repair of intima, reduces the new intima formation, and decreases the rate of restenosis.
Zhang
Xing-hua
XH
Department of Cardiology, Shandong Provincial Hospital, Jinan 250021, China.
Ma
Xiao-jing
XJ
Zhao
Tong
T
D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D023903:Coronary Restenosis / Q000517:prevention & control; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use*; D006965:Hyperplasia / Q000517:prevention & control; D007083:Iliac Artery / Q000473:pathology*; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D011897:Random Allocation; D017539:Tunica Intima / Q000473:pathology*
D016178:Granulocyte-Macrophage Colony-Stimulating Factor