title: [Evaluation of beta-lactamase detection reagent with used HMRZ-86 for the detection of extended-spectrum beta-lactamase and metallo-beta-lactamase].
abstract: Currently, evidence related to infectious diseases and the nosocomial infection in hospitals with Gram-negative rods with extended spectrum beta-lactamase (ESBL) or metallo-beta-lactamase (MBL) is accumulating. Thereby, it will be very important to know quickly the resistant mechanisms for antibiotics in the treatment strategy of patients with infectious diseases in a clinical setting. Then, beta-lactamase detection reagent Cica beta-Test (Kanto Chemical, Tokyo) which used HMRZ-86 was evaluated. Bacteria used in the present study were 42 stocked strains of beta-lactamase produced strain isolated from clinical samples in our hospital. ESBL were coincided in 13 strains among 17 (76.5%). MBL were coincided in 8 strains among 11 (72.7%). KOXY belongs to class A beta-lactamase was missjudged as ESBL. Therefore, although there are a little of limitations. Cica beta-Test provides us the rapid information of the esistance mechanism in 15-min with relatively, which is surely valuable to treat patients with bacterial infection and to prevent the nosocomial infection.
authors.affiliation: Division of Clinical Central Laboratory, Kansai Medical University Hospital, 10-15 Fumizono, Moriguchi, Osaka 570-8507, Japan. nakamurt@takii.kmc.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakamura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Uchida
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Tanaka
authors.forename: Miyuki
authors.initials: M
authors.lastname: Sano
authors.forename: Chiyo
authors.initials: C
authors.lastname: Nakata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Heijo
authors.forename: Hakuo
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D002511:Cephalosporins; D007202:Indicators and Reagents; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D002511:Cephalosporins; C487639:HMRZ 86; D007202:Indicators and Reagents; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases