title: An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging.
abstract: An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTF, and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540 nm with a peak intensity of around 490 nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation.
authors.affiliation: Center for Advanced Biomedical Photonics, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, USA.
authors.forename: Matt E
authors.initials: ME
authors.lastname: Martin
authors.forename: Musundi
authors.initials: M
authors.lastname: Wabuyele
authors.forename: Masoud
authors.initials: M
authors.lastname: Panjehpour
authors.forename: Bergein
authors.initials: B
authors.lastname: Overholt
authors.forename: Robert
authors.initials: R
authors.lastname: DeNovo
authors.forename: Steve
authors.initials: S
authors.lastname: Kennel
authors.forename: Glenn
authors.initials: G
authors.lastname: Cunningham
authors.forename: Tuan
authors.initials: T
authors.lastname: Vo-Dinh
mesh_terms: D000818:Animals; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007834:Lasers; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration