[Development of a multiantigenic serological test for tuberculosis diagnosis].
OBJECTIVE
The present work evaluated a multi-antigen printing immunoassay (MAPIA) for the serological diagnosis of tuberculosis.


METHODS
Sera were obtained from 66 patients with tuberculosis, verified clinically and bacteriologically and from 47 healthy individuals (control group). Sample sera were used for detection of antibodies against 3 enriched mixtures of proteins and 5 unique recombinant antigens. The antigens were presented in a solid matrix. Sensitivity, specificity and predictive values were evaluated and confirmed by a logistic regression analysis. A prevalence value was calculated and used for the selection of the best antigenic combination.


RESULTS
The sensitivity and specificity values of individual antigens varied between 5-83% and 9-100%. The enriched mixtures values were more accurate than those obtained with the recombinant antigens. Combinations of several antigens improved the sensitivity values up to the 81% level. In most cases, specificity values of 57% or less were obtained.


CONCLUSIONS
These results suggested that the multiantigenic test can be a useful screening tool, to be used in conjunction with the more definitive diagnostic tests.
Castro
Claudia Marcela
CM
Laboratorio de Micobacterias, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Porras
Tania Bibiana
TB
Guerrero
Martha Inirida
MI
León
Clara Ines
CI
Mojica
Maria Antonieta
MA
Lara
Martha
M
Parada
Maria Cristina
MC
Espitia
Clara
C
D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial