Monitoring dopamine release from single living vesicles with nanoelectrodes.
Carbon fiber nanoelectrodes (tip diameter = ca. 100 nm) have been first used to monitor real-time dopamine release from single living vesicles of single rat pheochromocytoma (PC12) cells. The experiments show that active and inactive release sites exist on the surface of cells, and the spatial distributions have been differentiated even in the same active release zone. It is first demonstrated that multiple vesicles can sequentially release dopamine at the same site of the cell surface, which possibly plays the main role in the dopamine release from PC12 cells.
Wu
Wen-Zhan
WZ
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Huang
Wei-Hua
WH
Wang
Wei
W
Wang
Zong-Li
ZL
Cheng
Jie-Ke
JK
Xu
Tao
T
Zhang
Rong-Ying
RY
Chen
Yu
Y
Liu
Jie
J
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002244:Carbon / Q000737:chemistry; D000077482:Carbon Fiber; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005089:Exocytosis / Q000502:physiology; D008839:Microelectrodes; D036103:Nanotechnology; D018377:Neurotransmitter Agents / Q000737:chemistry; D016716:PC12 Cells; D051381:Rats
D000077482:Carbon Fiber; D018377:Neurotransmitter Agents; D002244:Carbon; D004298:Dopamine