[Regulation of chromium on gene expression of skeletal muscles in diabetic rats].
OBJECTIVE
To study the regulation of chromium on gene expression related to metabolism of skeletal muscles in diabetic rats.


METHODS
Male Wistar rats were assigned to three groups: normal control group (NC), alloxan-induced diabetic control group (DM), and DM with chromium supplementation group (DM + Cr). 200 microg Cr/kg bw perday was supplemented orally for 60 days. At the end of the treatment, the changes in gene expression among three groups were studied by mRNA differential display technique combined with cDNA fragments cloning, sequencing and BLASTn analysis.


RESULTS
11 cDNA fragments larger than 400bp expressed differences in skeletal muscles between DM + Cr group and DM group were isolated, 4 of them expressed higher in DM + Cr group, while the rest expressed higher in DM group. Cr-3 and GLUT4 are homologous with sequence identities of 98% and Cr-5 and IRS-1 are homologous with sequence identities of 100%. The expression level of GLUT4 mRNA in DM + Cr group are higher than those of DM group.


CONCLUSIONS
Chromium supplementation could induce the expression of GLUT4 mRNA on skeletal muscles in diabetic rats. It may be one of the molecular mechanisms that chromium could improve the disorders of glucose and lipids metabolism in diabetic rats.
Wu
Yun-tang
YT
School of Public Health, Tianjin Medical University, Tianjin 300070, China. wuyuntang@tjmu.edu.cn
Sun
Zhong
Z
Che
Su-ping
SP
Wang
Xia
X
Wang
Yongming
Y
Guo
Gang
G
D000818:Animals; D002857:Chromium / Q000008:administration & dosage / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D051275:Glucose Transporter Type 4; D012333:RNA, Messenger; D002857:Chromium