Changes of the solution pH due to exposure by high-voltage electric pulses.
The change of the pH of a NaCl solution (139-149 mM NaCl) buffered with 5-15 mM sodium phosphates (pH 7.4) during electromanipulation was studied. It has been determined that an increase in the pH value of electroporation solution of a whole chamber volume, caused by the application of electric field pulses, commonly used in cell electromanipulation procedures, can exceed 1-2 pH units. Several materials for the cathode were tested. In all cases a stainless steel anode was utilized. The aluminum cathode gave a two-fold greater DeltapH in comparison with platinum, copper or stainless steel cathodes. In addition, a substantial release of aluminum (up to 1 mg/l) from the cathode was observed. It has also been found that the shift in pH depended on the medium conductivity: DeltapH of the solution, in which sucrose was substituted for NaCl, was about 5 times less. On the basis of the results obtained here, to avoid the plausible undesirable consequences of the cathodic electrolysis processes, in particular under the conditions of strong electric treatment, it could be recommended that chambers with aluminum electrodes not be utilized and one should use strongly buffered solutions of low conductivity and alternating current (sine or square wave) bipolar electric pulses.
Saulis
Gintautas
G
Department of Biology, Faculty of Nature Sciences, Vytautas Magnus University, 28 Daukanto str., 44246, Kaunas, Lithuania. sg@kaunas.omnitel.net
Lape
Remigijus
R
Praneviciūte
Rita
R
Mickevicius
Donatas
D
D004560:Electricity; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
D010710:Phosphates; D012996:Solutions; D012965:Sodium Chloride; C018279:sodium phosphate