title: A sex pheromone, protoplast release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium.
abstract: The sex pheromone protoplast release-inducing protein (PR-IP) inducer and a sexual cell division-inducing pheromone-minus (SCD-IP-minus) that mediates the sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale (C. psl) complex were investigated in this study. Recombinant PR-IP inducer produced by yeast cells was prepared and assayed for production of PR-IP and induction of SCD. Both biological activities were observed after treating mating-type plus (mt+) cells with the recombinant pheromone. SCD was induced by exposure to a lower concentration of the same pheromone and by a shorter treatment period with the pheromone than was production of PR-IP. This indicates that the previously characterized PR-IP inducer has both PR-IP-inducing and SCD-inducing activities with mt+ cells, although the inducing mechanisms of the two pheromones differ.
authors.affiliation: Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902 Japan. tsuchik@dolphin.c.u-tokyo.ac.jp
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tsuchikane
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fujii
authors.forename: Motomi
authors.initials: M
authors.lastname: Ito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sekimoto
mesh_terms: D002455:Cell Division / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D056890:Eukaryota / Q000166:cytology / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis / Q000502:physiology*; D012724:Sex Attractants / Q000502:physiology*
chemical_list: C088218:19-KSU protein, Closterium peracerosum; D010940:Plant Proteins; D012724:Sex Attractants