Establishment and characterization of new cell lines derived from melanotic neuroectodermal tumor of infancy arising in the mandible.
Three cell systems (MINT1/2/3) derived from a melanotic neuroectodermal tumor of infancy (MNTI) arising in the mandible of a 1-month-old newborn boy have been established, and their cytological natures have been characterized. The cells had immunopositivities for pan-keratin, vimentin, neuron-specific enolase, S-100 protein and melanoma-associated antigen (HMB-45). These immunohistochemical phenotypes were basically the same as those observed in tissue sections, in which, synaptophysin, myelin basic protein, c-myc gene products, carcinoembryonic antigen, and epithelial membrane antigen were also immunolocalized in tumor cells. Karyotyping analyzes revealed that the chromosome numbers of the three cell systems ranged from 60 to 67 with 3n ploidies, and that there were many structural aberrations, such as del(11)(q13), del(22)(q13), add(2)(p11), add(7)(q22), extra copies for chromosomes 1, 2, 3, 5, 7, 9, 10, 11, 12, 16, 20, and 22, der(9)t(9;13)(p13;q12)add(9)(q34), and der(13;21)(q10;q10), which were shared by the three cell systems, while der(19)t(11;19)(q13;p13) was found in MINT1 and MINT3. When stimulated by endothelin-3 and vitamin D(3), the cells had spinous cell shapes with immunopositivities for HMB-45, neurofilament protein and glial fibrillary acidic protein, which indicated more neural differentiation. The established cell systems will be useful for further investigation on the molecular and genetic basis of MNTI to understand its pathogenesis, which is largely unknown.
Metwaly
Hamdy
H
Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Cheng
Jun
J
Maruyama
Satoshi
S
Ohshiro
Kazufumi
K
Suzuki
Ichiro
I
Hoshina
Yukiko
Y
Saku
Takashi
T
D000818:Animals; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002762:Cholecalciferol / Q000494:pharmacology; D002869:Chromosome Aberrations; D019334:Endothelin-3 / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D058950:Melanoma-Specific Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018396:Mucin-1 / Q000032:analysis; D004676:Myelin Basic Protein / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D017600:Neuroectodermal Tumor, Melanotic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D014183:Transplantation, Heterologous; D014746:Vimentin / Q000032:analysis
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D019334:Endothelin-3; C489427:MYC protein, human; D058950:Melanoma-Specific Antigens; D018396:Mucin-1; D004676:Myelin Basic Protein; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D009418:S100 Proteins; D014746:Vimentin; D002762:Cholecalciferol; D007633:Keratins; D010751:Phosphopyruvate Hydratase