title: TRPC channels as signal transducers.
abstract: The study of the TRPC cation channels as signal transducers in sensory neurons is in its infancy. Mechanoreceptors that monitor arterial pressure are prime candidates for the involvement of TRPC channels as either primary mechanical transducers or as modulators of the transduction process. Their activity patterns can be regulated by growth factors such as BDNF and by a variety of ligands that activate Gq-coupled receptors, mechanisms that have been shown in heterologous expression systems to activate TRPC channels. We investigated the distribution of TRPC1 and TRPC3-7 in nodose sensory neurons and in their peripheral axons that terminate as mechanosensitive receptors in the aortic arch of the rat. Using immunocytochemical techniques we identified these six TRPC proteins in the soma of the nodose neurons but only TRPC1 and TRPC3-5 were found to distribute to the peripheral axons and the mechanosensory terminals. TRPC1 and TRPC3 extended into the low threshold complex sensory endings with very strong labeling. In contrast, TRPC4 and TRPC5 were found primarily in major branches of the receptor but immunoreactivity was weak in the region where mechanotransduction is presumed to occur. Terminals arising from unmyelinated fibers also expressed TRPC1 and TRPC3-5 but not all fibers expressed all of the channels suggesting that specific TRPC protein may be aligned with previously described subclasses of the unmyelinated C-fibers.
authors.affiliation: Rammelkamp Center for Education and Research, Case Western Reserve University, MetroHealth Campus, 2500 MetroHealth Drive, Cleveland, OH 44109, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Glazebrook
authors.forename: William P
authors.initials: WP
authors.lastname: Schilling
authors.forename: Diana L
authors.initials: DL
authors.lastname: Kunze
mesh_terms: D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D008465:Mechanoreceptors / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009620:Nodose Ganglion / Q000502:physiology; D011311:Pressoreceptors / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology*; D050052:TRPC Cation Channels / Q000502:physiology*
chemical_list: D050052:TRPC Cation Channels