title: Real-time detection of virus particles and viral protein expression with two-color nanoparticle probes.
abstract: Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.
authors.affiliation: Department of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, Atlanta, Georgia 30322, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Agrawal
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
authors.forename: Shuming
authors.initials: S
authors.lastname: Nie
mesh_terms: D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D012996:Solutions; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification; D014773:Virology / Q000379:methods
chemical_list: D012996:Solutions; D014764:Viral Proteins