title: Industrial process proteomics: alfalfa protein patterns during wet fractionation processing.
abstract: A proteome reference map of major soluble proteins from Medicago sativa (alfalfa) leaves and stems has been established for the first time. Among 195 spots analyzed by mass spectrometry and N-terminal Edman sequencing, 117 spots were unambiguously identified, representing 87 different proteins. Of these 87 proteins, 13 proteins were directly identified from the partial genome of Medicago sativa, 30 from expressed sequenced tags (ESTs) of the model legume Medicago truncatula and 44 from closely relative species by a cross-species protein identification method. The proteome map of Medicago sativa was then set as a reference to study the major high protein content products that are generated during the wet fractionation process of alfalfa green biomass. Using two-dimensional electrophoresis, we studied the variation of the protein patterns at different steps of the industrial-scale process. We clearly show that the process induces significant changes including chemical modifications, proteolytic events, and heat-shock protein responses. Strikingly, a certain level of cellular regulation is conserved during biomass processing, as exemplified by the induction of some heat shock proteins. Finally, all the results obtained in this proteomic study may help to identify novel products and to improve process designs in alfalfa biomass plants.
authors.affiliation: Viridis S.A., Aulnay-aux-Planches, F-51130 Val des Marais, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Incamps
authors.forename: Florence
authors.initials: F
authors.lastname: Hély-Joly
authors.forename: Pierre
authors.initials: P
authors.lastname: Chagvardieff
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rambourg
authors.forename: Alain
authors.initials: A
authors.lastname: Dedieu
authors.forename: Elodie
authors.initials: E
authors.lastname: Linares
authors.forename: Eric
authors.initials: E
authors.lastname: Quéméneur
mesh_terms: D005591:Chemical Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D007218:Industrial Microbiology / Q000379:methods*; D000455:Medicago sativa / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D020033:Protein Isoforms / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D016415:Sequence Alignment
chemical_list: D020033:Protein Isoforms; D011506:Proteins