title: Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos.
abstract: OBJECTIVE
  To investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome (BWS) in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS.
  
  
  METHODS
  Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
  
  
  RESULTS
  Transcripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos.
  
  
  CONCLUSIONS
  Transcripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.
authors.affiliation: Reproductive Medical Center, Nanfang Hospital, Guangzhou, Guangdong, 510515, PR China.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Shen
authors.forename: Fu-qi
authors.initials: FQ
authors.lastname: Xing
authors.forename: Ling-hong
authors.initials: LH
authors.lastname: Kong
authors.forename: Shi-ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D001506:Beckwith-Wiedemann Syndrome / Q000235:genetics*; D001755:Blastocyst / Q000378:metabolism*; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics; D009865:Oocytes / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050761:Cyclin-Dependent Kinase Inhibitor p57; C485621:KCNQ1OT1 long non-coding RNA, human; D009687:Nuclear Proteins; D024642:Potassium Channels, Voltage-Gated; C109277:TSSC3 protein