Whole-genome transcription profiling of rhesus monkey rhadinovirus.
Rhesus monkey rhadinovirus (RRV) and Kaposi's sarcoma-associated herpesvirus (KSHV; also called human herpesvirus 8) belong to the gamma-2 grouping of herpesviruses. RRV and KSHV share a high degree of sequence similarity, and their genomes are organized in a similar fashion. RRV serves as an excellent animal model system to study the gamma herpesvirus life cycle both in vitro and in vivo. We have developed a high-sensitivity, high-throughput, high-specificity real-time quantitative reverse transcriptase-based PCR assay for RRV and have used this assay to profile transcription from the whole RRV genome during de novo productive infection of rhesus fibroblasts. Using this assay, we demonstrate that the genome-wide transcription profile for RRV closely parallels the genome-wide transcription profile for KSHV.
Dittmer
Dirk P
DP
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, North Carolina 27599, USA.
Gonzalez
Carlos M
CM
Vahrson
Wolfgang
W
DeWire
Scott M
SM
Hines-Boykin
Rebecca
R
Damania
Blossom
B
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D016679:Genome, Viral; D008253:Macaca mulatta / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D018144:Rhadinovirus / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D017931:DNA Primers