Identification of a lactose-responsive element upstream of the promoter of Bacillus megaterium beta-galactosidase-encoding gene mbgA.
The Bacillus megaterium mbgA gene encodes a lactose-hydrolyzing beta-galactosidase. An AraC/XylS-type activator BgaR can activate mbgA transcription in response to lactose. In this report, we show by various deletion analyses and point mutagenesis analyses that an inverted repeat centered at position -60.5 relative to the mbgA transcriptional initiation site is the cis-acting element responsible for lactose induction of mbgA expression.
Li
Jen-Ming
JM
Institute of Biochemistry and Molecular Biology, School of Life Science, National Yang-Ming University, Taipei, Taiwan, ROC.
Chiou
Chih-Yung
CY
Lee
Tian-Ren
TR
Chen
Yuan-Shou
YS
Shaw
Gwo-Chyuan
GC
D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D001616:beta-Galactosidase; D007785:Lactose