Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors.
In spite of their apparently restricted differentiation potentiality, hematopoietic precursors are plastic cells able to trans-differentiate from a maturation lineage to another. To better characterize this differentiation plasticity, we purified CD14- and CD14+ myeloid precursors generated by 'in vitro' culture of human CD34+ hematopoietic progenitors. Morphological analysis of the investigated cell populations indicated that, as expected, they consisted of granulocyte and monocyte precursors, respectively. Treatment with differentiation inducers revealed that CD14- cells were bipotent granulo-monocyte precursors, while CD14+ cells appeared univocally committed to a terminal macrophage maturation. Flow cytometry analysis demonstrated that the conversion of granulocyte precursors to the mono-macrophage maturation lineage occurs through a differentiation transition in which the granulocyte-related myeloperoxidase enzyme and the monocyte-specific CD14 antigen are co-expressed. Expression profiling evidenced that the observed trans-differentiation process was accompanied by a remarkable upregulation of the monocyte-related MafB transcription factor.
Montanari
M
M
Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Università di Modena e Reggio Emilia, Modena, Italy.
Gemelli
C
C
Tenedini
E
E
Zanocco Marani
T
T
Vignudelli
T
T
Siena
M
M
Zini
R
R
Salati
S
S
Chiossi
G
G
Tagliafico
E
E
Manfredini
R
R
Grande
A
A
Ferrari
S
S
D018952:Antigens, CD34 / Q000276:immunology*; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage; D002478:Cells, Cultured; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D051258:MafB Transcription Factor / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism*
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D018950:Lipopolysaccharide Receptors; C490407:MAFB protein, human; D051258:MafB Transcription Factor; D012333:RNA, Messenger