Structure, gene expression, and evolution of primate glutathione peroxidases.
Glutathione peroxidases (GPxs) are a family of enzymes that scavenge peroxides generated in cells. We carried out molecular cloning for cDNAs of four GPx isozymes (GPx-1 through 4) in primate species. The essential residues for the function of these isozymes were well conserved. A phylogenetic tree of GPx isozymes of primates and other mammals showed that GPx-4 diverged first, followed by GPx-3, GPx-2, and GPx-1. Expression of mRNAs for GPx-2 through 4 in various tissues of Japanese monkey was analyzed by Northern blot hybridization. GPx-2 mRNA was detected at 1.7 kb, exclusively in the stomach and small intestine. GPx-3 mRNA was detected at 1.8 kb, intensively in the kidney and adrenal gland, and weakly in the cerebellum, heart, and lung. GPx-4 mRNA was detected at 1.1 kb, very intensively in the testis and weakly in lung, heart, and cerebellum. These results showed that GPx isozymes were expressed under tissue-specific regulations.
Fukuhara
Ryoji
R
Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama Aichi, 484-8506, Japan. fukuhara@pri.kyoto-u.ac.jp
Kageyama
Takashi
T
D000595:Amino Acid Sequence; D000818:Animals; D002428:Cebus; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D006922:Hylobates; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008251:Macaca; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011148:Pongo pygmaeus; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D005979:Glutathione Peroxidase