title: Inhibitory effect on proliferation of vascular smooth muscle cells and protective effect on CCl(4)-induced hepatic damage of HEAI extract.
abstract: The effects of methanol extract from Hericium erinaceus cultivated with Artemisia iwayomogi (HEAI) on proliferation of vascular smooth muscle cells and CCl(4)-induced hepatic damage were evaluated. HEAI was shown to have a potent inhibitory effect on the proliferation of vascular smooth muscle cells (VSMCs). Interestingly, a methanol extract of Hericium erinaceus showed no inhibitory effect on the proliferation of VSMCs, while a methanol extract of Artemisia iwayomogi possessed strong inhibitory effects on the proliferation of VSMCs. Therefore, the inhibitory effects of HEAI may be caused by the changes of chemical components in the culture broth after the addition of Artemisia iwayomogi in the HEAI growth media. HEAI also had a strong protective effect on CCl(4)-induced hepatic damage in rats. The activity was evaluated using biochemical parameters such as glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and alkaline phosphatase (ALP). HEAI treatment caused a significant reduction in the activity of GOT but not of GPT and ALP in comparison with CCl(4) treatment alone. Histopathological studies showed that liver samples treated with HEAI were significantly different when compared to non-treated animals after CCl(4) exposure.
authors.affiliation: Department of Life Sciences, Soonchunhyang University, Asan, Chungnam 336-745, Korea.
authors.forename: Won-Sik
authors.initials: WS
authors.lastname: Choi
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.forename: Byeoung-Soo
authors.initials: BS
authors.lastname: Park
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Gary R
authors.initials: GR
authors.lastname: Takeoka
authors.forename: Dong-Goo
authors.initials: DG
authors.lastname: Kim
authors.forename: Xiang
authors.initials: X
authors.lastname: Lanpiao
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors; D049109:Cell Proliferation / Q000187:drug effects*; D056486:Chemical and Drug Induced Liver Injury; D004305:Dose-Response Relationship, Drug; D008107:Liver Diseases / Q000188:drug therapy*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D010936:Plant Extracts; D002251:Carbon Tetrachloride