[Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice].
It is critical to generate marker gene free transgenic plants for retransformating or eliminating the potential harmfulness of marker gene and its product. In this study, Ac/Ds transposon system was developed for removal of hpt selection marker gene to obtain marker-free transgenic plants in rice ( Oryza sativa L.). Ds element containing the interesting gene bar was constructed next to the selection marker gene hpt to get Ds-T-DNA. Rice plants were transformed by Agrobacterium tumefaciens EHA105 containing Ac-T-DNA and Ds-T-DNA respectively. Rice plant containing single copy Ac-T-DNA was crossed with plant containing single copy Ds-T-DNA to obtain the F1 plant containing both Ac and Ds elements. F1 plant was self-crossed to produce F2 progeny in which T-DNA insert and transposed Ds element segregated independently. Two plants contained Ds element but no hpt marker gene in total 100 F2 plants. The result indicated that Ac/Ds transposon system could be used as a vector system for generating marker gene free transgenic plants in rice.
Jin
Wei-Zheng
WZ
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hanrgzhou 310029, China.
Duan
Rui-Jun
RJ
Zhang
Fan
F
Chen
Shuang-Yan
SY
Wu
Yun-Rong
YR
Wu
Ping
P
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015139:Blotting, Southern; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D004251:DNA Transposable Elements