Microarrays and expression profiling in microglia research and in inflammatory brain disorders.
Expression profiling by using microarrays is a powerful tool for investigating transcriptional changes in a variety of diseases. In this survey, microarray data selected from the literature from in vivo and in vitro studies are scrutinized to find differentially expressed genes in common within specific inflammatory conditions in brain or microglial cell cultures, if there are at least two independent investigations available. Viral encephalitis, multiple sclerosis, epileptic seizures, ischemic lesions, and traumatic brain injury are the disorders covered. Moreover, by taking into account expression data obtained from cultured microglia, two examples are presented of how one can deal (or should not deal) with lists of candidate genes showing up in these kinds of studies without sophisticated software programs. Finally, some general remarks are made about pivotal issues when beginning to use microarray technology.
Gebicke-Haerter
Peter J
PJ
Department of Psychopharmacology, Central Institute for Mental Health, Mannheim, Germany. gebicke@zi-mannheim.de
D000818:Animals; D017209:Apoptosis; D001927:Brain Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D046228:Microarray Analysis / Q000379:methods; D017628:Microglia / Q000378:metabolism*; D008954:Models, Biological