title: High-density electrode array for imaging in vitro electrophysiological activity.
abstract: The development of a high-density active microelectrode array for in vitro electrophysiology is reported. Based on the Active Pixel Sensor (APS) concept, the array integrates 4096 gold microelectrodes (electrode separation 20 microm) on a surface of 2.5 mmx2.5 mm as well as a high-speed random addressing logic allowing the sequential selection of the measuring pixels. Following the electrical characterization in a phosphate solution, the functional evaluation has been carried out by recording the spontaneous electrical activity of neonatal rat cardiomyocytes. Signals with amplitudes from 130 microVp-p to 300 microVp-p could be recorded from different pixels. The results demonstrate the suitability of the APS concept for developing a new generation of high-resolution extracellular recording devices for in vitro electrophysiology.
authors.affiliation: Sensors, Actuators and Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland. luca.berdondini@unine.ch
authors.forename: L
authors.initials: L
authors.lastname: Berdondini
authors.forename: P D
authors.initials: PD
authors.lastname: van der Wal
authors.forename: O
authors.initials: O
authors.lastname: Guenat
authors.forename: N F
authors.initials: NF
authors.lastname: de Rooij
authors.forename: M
authors.initials: M
authors.lastname: Koudelka-Hep
authors.forename: P
authors.initials: P
authors.lastname: Seitz
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: P
authors.initials: P
authors.lastname: Metzler
authors.forename: N
authors.initials: N
authors.lastname: Blanc
authors.forename: S
authors.initials: S
authors.lastname: Rohr
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation*; D006046:Gold; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000502:physiology; D051381:Rats
chemical_list: D006046:Gold