title: ZNF649, a novel Kruppel type zinc-finger protein, functions as a transcriptional suppressor.
abstract: Cardiac differentiation involves a cascade of coordinated gene expression that regulates cell proliferation and matrix protein formation in a defined temporo-spatial manner. Many of the KRAB-ZFPs are involved in cardiac development or cardiovascular diseases. Here we report the identification and characterization of a novel human zinc-finger gene named ZNF649. The cDNA of ZNF649 is 3176 bp, encoding a protein of 505 amino acids in the nuclei. Northern blot analysis indicates that ZNF649 is expressed in most of the examined human adult and embryonic tissues. ZNF649 is a transcription suppressor when fused to GAL-4 DNA-binding domain and cotransfected with VP-16. Overexpression of ZNF649 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1. Deletion analysis with a series of truncated fusion proteins indicates that the KRAB motif is a basal repression domain when the truncated fusion proteins were assayed for the transcriptional activities of SRE and AP-1. These results suggest that ZNF649 protein may act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway to mediate cellular functions.
authors.affiliation: The Center for Heart Development, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Zhu
authors.forename: Bisheng
authors.initials: B
authors.lastname: Liu
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Canding
authors.initials: C
authors.lastname: Wang
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D050980:GATA Transcription Factors; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012097:Repressor Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013045:Species Specificity; D014018:Tissue Distribution; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D015533:Transcriptional Activation / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D050980:GATA Transcription Factors; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; C115209:Sre protein, Neurospora crassa; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C502775:ZNF649 protein, human