title: Milk-derived GM(3) and GD(3) differentially inhibit dendritic cell maturation and effector functionalities.
abstract: Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides in breast milk has yet to be elucidated but when milk is ingested, dietary gangliosides might conceptually affect immune cells, such as dendritic cells (DCs). In this study, we address the in vitro effect of GD(3) and GM(3) on DC effector functionalities. Treatment of bone marrow-derived DCs with GD(3) before lipopolysaccharide-induced maturation decreased the production of interleukin-6 (IL-6), IL-10, IL-12 and tumor necrosis factor-alpha as well as reduced the alloreactivity in mixed leucocyte reaction (MLR). In contrast, only IL-10 and IL-12 productions were significantly inhibited by GM(3,) and the potency of DCs to activate CD4(+) cells in MLR was unaffected by GM(3). However, both gangliosides suppressed expression of CD40, CD80, CD86 and major histocompatibility complex class II on DCs. Because GD(3) overall inhibits DC functionalities more than GM(3), the immune modulating effect of the ganglioside fraction of breast milk might be more prominent in the commencement of lactation during which the milk contains the most GD(3).
authors.affiliation: Biochemistry and Nutrition Group, Centre for Advanced Food Studies and Biocentrum-DTU, Technical University of Denmark, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Brønnum
authors.forename: T
authors.initials: T
authors.lastname: Seested
authors.forename: L I
authors.initials: LI
authors.lastname: Hellgren
authors.forename: S
authors.initials: S
authors.lastname: Brix
authors.forename: H
authors.initials: H
authors.lastname: Frøkiaer
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology*; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008892:Milk / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D005679:G(M3) Ganglioside; D005732:Gangliosides; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016753:Interleukin-10; D018664:Interleukin-12; C026226:ganglioside, GD3