Alterations of glomerular and extracellular levels of glutathione peroxidase in patients and experimental rats with diabetic nephropathy.
To investigate the status and role of glutathione peroxidase (GPX) in diabetic nephropathy, we measured GPX in the plasma and urine of 14 patients with diabetic glomerulosclerosis (DGS) and measured glomerular GPX immunostaining in these patients and in rats with streptozotocin-induced diabetes of varying duration. Plasma GPX levels were significantly lower in DGS patients than in diabetic patients without nephropathy (P < .05) or normal controls (P < .01). Urinary GPX concentrations were also significantly lower in DGS patients than in diabetic patients without nephropathy or normal controls (both P < .05). Immunostaining of glomerular GPX was significantly less in DGS patients than in normal controls (P < .05) and was negatively correlated with the glomerular sclerosis score and the index of mesangial expansion. Serial examination of glomerular GPX in diabetic rats showed that immunostaining scores for glomerular GPX in rats were significantly lower than those in normal control rats after 1 and 3 months' duration of diabetes, and staining scores were also significantly lower in rats killed after 3 months of diabetes than in those killed after 1 week. In conclusion, our study demonstrates that GPX concentrations in plasma, urine, and glomeruli are decreased in individuals with DGS and that the immunostaining of glomerular GPX decreases progressively.
Chiu
Yi-Wen
YW
Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University, Taiwan.
Kuo
Mei-Chuan
MC
Kuo
Hung-Tien
HT
Chang
Jer-Ming
JM
Guh
Jinn-Yuh
JY
Lai
Yung-Hsiung
YH
Chen
Hung-Chun
HC
D000328:Adult; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D003928:Diabetic Nephropathies / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood* / Q000652:urine*; D006801:Humans; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar
D005979:Glutathione Peroxidase