Anti-inflammatory and antimicrobial effect of lectin from Lonchocarpus sericeus seeds in an experimental rat model of infectious peritonitis.
We have investigated the anti-inflammatory and antimicrobial effect of the lectin from Lonchocarpus sericeus seeds (LSL) in a model of infectious peritonitis in adult Wistar rats. Animals were treated with saline or LSL (10 mg kg(-1), i.v) immediately and 6 h after the induction of peritonitis via cecal ligation and single puncture. Twelve hours after surgery, animals were killed and the infectious process was monitored by total and differential count of cells from blood and peritoneal washing liquid, adenosine deaminase activity, antibiogram and the number of viable bacteria of the peritoneal cavity. LSL treatment decreased the inflammatory response evoked by the induction of peritonitis, as seen by the inhibition of neutrophil migration into peritoneal cavities, leucocytosis and reduction of adenosine deaminase activity in the peritoneal fluid. All these effects were reversed by the lectin association to N-acetyl-glucosamine. LSL in-vitro did not show any antimicrobial action, but promoted a marked decrease of the viable bacterial population in peritoneal cavities. In conclusion, LSL inhibited the inflammatory response and the bacterial colonization of infectious peritonitis in rats.
Alencar
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NM
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Brazil. Cel Nunes de Melo, 1127, 60430-270, Fortaleza-Ceará-Brasil. nylane@ufc.br
Cavalcante
C F
CF
Vasconcelos
M P
MP
Leite
K B
KB
Aragão
K S
KS
Assreuy
A M S
AM
Nogueira
N A P
NA
Cavada
B S
BS
Vale
M R
MR
D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement; D029866:Derris / Q000737:chemistry*; D007249:Inflammation; D037102:Lectins; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010538:Peritonitis / Q000188:drug therapy* / Q000662:veterinary; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry
D037102:Lectins; D010936:Plant Extracts