[Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea].
The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T-vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT-PCR. Sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from Beta vulgaris, Nicotiana, wheat and maize mitochondrion, respectively. This analysis suggests that orf25 gene is highly conserved in terms of evolution in plant; and it also suggests that wild plant Salicornia europaea contain a male-sterility gene similar to crops that is of great importance in improvement of the breed of crop.
Li
Jin-Yao
JY
College of Life science and Technology, Xinjiang University, Urumuqi 830046, China.
Zhang
Fu-Chun
FC
Ma
Ji
J
Shan
Wen-Juan
WJ
Wang
Bin
B
D001483:Base Sequence; D019663:Chenopodiaceae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D008969:Molecular Sequence Data; D051479:Plant Infertility / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D010940:Plant Proteins