title: Integration of mutations responsible for the attenuated phenotype of Pepper mild mottle virus strains results in a symptomless cross-protecting strain.
abstract: An enhanced attenuated strain of Pepper mild mottle virus (PMMoV) was constructed by incorporating mutations that affect viral attenuation from three reported attenuated strains of PMMoV, which causes serious economic losses in the production of green pepper in Japan. The new strain caused no symptoms on pepper plants and protected them from infection by a wild-type strain. The mutations responsible for viral attenuation were located in the intervening region (IR) of the 126-kDa/183-kDa proteins. The mutations had synergistic effects in terms of the attenuation of symptoms and decreased the accumulation of the viral coat protein in infected pepper plants. In this paper, we propose an efficient method for the improvement of attenuated viruses by reverse genetics in plant viruses.
authors.affiliation: National Agricultural Research Center, Tsukuba, Ibaraki, Japan. tuehara@affrc.go.jp
authors.forename: T U
authors.initials: TU
authors.lastname: Ichiki
authors.forename: E N
authors.initials: EN
authors.lastname: Nagaoka
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: K
authors.initials: K
authors.lastname: Uchikawa
authors.forename: S
authors.initials: S
authors.lastname: Tsuda
authors.forename: T
authors.initials: T
authors.lastname: Omura
mesh_terms: D001483:Base Sequence; D002212:Capsicum / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007564:Japan; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins; D017898:Tobamovirus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D018076:DNA, Complementary; D004279:DNA, Viral; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins