title: Bee venom modulates murine Th1/Th2 lineage development.
abstract: Administration of bee venom (BV) elicits anti-inflammatory, anti-nociceptive and anti-allergic effects in various animal models. This study was designed to evaluate the direct effects of BV on helper T cell activities and on Th1/Th2 lineage development using both in vitro and in vivo conditions. In the Th1 skewed condition, BV increased the expression of IFN-gamma mRNA and enhanced the expression of T-bet on purified CD4(+) T cells from splenocytes of BALB/c mice. On the other hand, BV treatment did not alter the expression of IL-4 or GATA-3 in a Th2 driven environment. To elucidate the effects of BV on Th1/Th2 lineage development under in vivo conditions, BV was given by intraperitonial injection to BALB/c mice. It significantly increased the CD4(+) T cell population and enhanced IFN-gamma expression, while IL-4 transcripts were not altered upon in vivo activation using an anti-CD3 antibody injection. Taken together, these results imply that BV induces Th1 lineage development from CD4(+) T cells by increasing the expression of a Th1-specific cytokine, IFN-gamma. In addition, this result may be mediated by inducing a Th1-specific transcription factor, T-bet.
authors.affiliation: College of Oriental Medicine, Kyung Hee University, #1 Hoeki-dong Dongdaemoon-gu, Seoul, 130-701, Korea.
authors.forename: Sangsoo
authors.initials: S
authors.lastname: Nam
authors.forename: Eunjung
authors.initials: E
authors.lastname: Ko
authors.forename: Seong-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Seonggyu
authors.initials: S
authors.lastname: Ko
authors.forename: Chan-Yong
authors.initials: CY
authors.lastname: Jun
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Shin
authors.forename: Moo-Chang
authors.initials: MC
authors.lastname: Hong
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D019070:Cell Lineage / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000032:analysis; D050990:GATA3 Transcription Factor; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D051379:Mice; D020825:T-Box Domain Proteins; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*; D015534:Trans-Activators / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis; D000098815:T-bet Transcription Factor
chemical_list: D001514:Bee Venoms; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; D015847:Interleukin-4; D007371:Interferon-gamma