Short tandem repeat DNA typing provides an international reference standard for authentication of human cell lines.
Cell lines have wide applications as model systems in the medical and pharmaceutical industry. Much drug and chemical testing is now first carried out exhaustively on in vitro systems, reducing the need for complicated and invasive animal experiments. The basis for any research, development or production program involving cell lines is the choice of an authentic cell line. Microsatellites in the human genome that harbour short tandem repeat (STR) DNA markers allow individualisation of established cell lines at the DNA level. Fluorescence polymerase chain reaction amplification of eight highly polymorphic microsatellite STR loci plus gender determination was found to be the best tool to screen the uniqueness of DNA profiles in a fingerprint database. Our results demonstrate that cross-contamination and misidentification remain chronic problems in the use of human continuous cell lines. The combination of rapidly generated DNA types based on single-locus STR and their authentication or individualisation by screening the fingerprint database constitutes a highly reliable and robust method for the identification and verification of cell lines.
Dirks
Wilhelm Gerhard
WG
German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Cultures, D-Braunschweig. WDI@DSMZ.de
Faehnrich
Silke
S
Estella
Isabelle Annick Janine
IA
Drexler
Hans Guenter
HG
D000826:Animal Testing Alternatives / Q000592:standards; D002460:Cell Line; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D030561:Databases, Nucleic Acid; D006801:Humans; D007621:Karyotyping; D009369:Neoplasms / Q000235:genetics; D012015:Reference Standards; D015203:Reproducibility of Results; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004247:DNA