title: DNA methylation increases throughout Arabidopsis development.
abstract: We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.
authors.affiliation: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Ruiz-García
authors.forename: M T
authors.initials: MT
authors.lastname: Cervera
authors.forename: J M
authors.initials: JM
authors.lastname: Martínez-Zapater
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant
chemical_list: D018744:DNA, Plant