A novel simian immunodeficiency virus from black mangabey (Lophocebus aterrimus) in the Democratic Republic of Congo.
In order to understand primate lentivirus evolution, characterization of additional simian immunodeficiency virus (SIV) strains is essential. Here, an SIV from a black mangabey (Lophocebus aterrimus) originating from the Democratic Republic of Congo was analysed phylogenetically. The monkey had cross-reactive antibodies against human immunodeficiency virus type 1 (HIV-1) and HIV-2. The viral pol region sequence was amplified by nested PCR and sequence analysis confirmed that it was related to known SIV sequences. This is the first report to characterize genetically an SIV from the monkey genus Lophocebus. Phylogenetic analysis of the pol region revealed that this novel SIV, designated SIVbkm, fell into the SIVsyk and SIVgsn virus group, containing viruses isolated from the genus Cercopithecus, and suggests that cross-species transmission has occurred between species of the genera Lophocebus and Cercopithecus.
Takemura
Taichiro
T
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Ekwalanga
Michel
M
National Institute of Biomedical Research, Kinshasa, Democratic Republic of Congo.
Bikandou
Blaise
B
Cite Louis Pasteur-Laboratoire National de Sante Publique, Brazzaville 120, Republic of Congo.
Ido
Eiji
E
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Yamaguchi-Kabata
Yumi
Y
Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Japan.
Ohkura
Sadayuki
S
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Harada
Hirotada
H
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Takehisa
Jun
J
Department of Viral Infection and International Health, Graduate School of Medical Science, Kanazawa University, Japan.
Ichimura
Hiroshi
H
Department of Viral Infection and International Health, Graduate School of Medical Science, Kanazawa University, Japan.
Parra
Henri-Joseph
HJ
Cite Louis Pasteur-Laboratoire National de Sante Publique, Brazzaville 120, Republic of Congo.
Nende
Monique
M
Kinshasa Zoo, Kinshasa, Democratic Republic of Congo.
Mubwo
Eric
E
Kinshasa Zoo, Kinshasa, Democratic Republic of Congo.
Sepole
Midingi
M
Kinshasa Zoo, Kinshasa, Democratic Republic of Congo.
Hayami
Masanori
M
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Miura
Tomoyuki
T
Institute for Virus Research, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
D000818:Animals; D016664:Cercocebus / Q000821:virology*; D002521:Cercopithecus / Q000821:virology; D015023:Democratic Republic of the Congo; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification