Methodologic factors affect the measurement of anti-basal ganglia antibodies.
An autoimmune etiology has been proposed for a variety of movement disorders, making the detection of autoantibodies a high investigative priority. Recognizing the existence of different methodologic approaches to identify these antibodies, we sought to investigate the effects of tissue preparation, antibody selection, and Western immunoblot detection methods on outcome. ELISA and immunoblotting studies were performed in healthy controls evaluating non-pathogenic autoantibodies. Our results indicate that enhanced data can be obtained by using fresh, rather than frozen, postmortem tissue homogenates for Western immunoblots and enzyme-linked immunosorbent assays and support the use of electrochemiluminescent detection for Western immunoblots. Molecular localization is significantly affected by the selected standard. Removal of lipids from homogenates does not affect anti-basal ganglia antibody (ABGA) results. Methodological variables should be taken into consideration when performing and interpreting neuroimmunological assays using sera or isolated IgG.
Rippel
Christopher A
CA
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hong
John J
JJ
Yoon
Dustin Y
DY
Williams
Phillip N
PN
Singer
Harvey S
HS
D000328:Adult; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D001479:Basal Ganglia / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male
D001323:Autoantibodies; D007074:Immunoglobulin G