[Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos].
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
Yu
Wei-Dong
WD
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduated School of Chinese Academy of Sciences, Beijing 100080, China.
Li
Wen-Yong
WY
Wang
Yu-Ge
YG
Yang
Li-Xin
LX
Liu
Gui-Sheng
GS
Du
Miao
M
Chen
Qing-Xuan
QX
D000818:Animals; D001755:Blastocyst / Q000378:metabolism* / Q000502:physiology; D015152:Blotting, Northern; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011817:Rabbits