title: Structural conservation of residues in BH1 and BH2 domains of Bcl-2 family proteins.
abstract: The sequence of Bcl-2 homology domains, BH1 and BH2, is known to be conserved among anti- and pro-apoptotic members of Bcl-2 family proteins. But structural conservation of these domains with respect to functionally active residues playing role in heterodimerization-mediated regulation of apoptosis has never been elucidated. Here, we have suggested the formation of an active site by structurally conserved residues in BH1 (glycine, arginine) and BH2 (tryptophan) domains of Bcl-2 family members, which also accounts for the functional effect of known mutations in BH1 (G145A, G145E) and BH2 (W188A) domains of Bcl-2.
authors.affiliation: Stem Cell Gene Therapy Research Group, Institute of Nuclear Medicine and Allied Sciences, DRDO, Delhi 110054, India. gugdutta@rediffmail.com
authors.forename: Gangenahalli U
authors.initials: GU
authors.lastname: Gurudutta
authors.forename: Yogesh Kr
authors.initials: YK
authors.lastname: Verma
authors.forename: Vimal Kishor
authors.initials: VK
authors.lastname: Singh
authors.forename: Pallavi
authors.initials: P
authors.lastname: Gupta
authors.forename: H G
authors.initials: HG
authors.lastname: Raj
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D017124:Conserved Sequence; D019281:Dimerization; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry* / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2