title: Adjuvant effect of Taenia crassiceps glycans against leishmanial antigens in mice infected with Leishmania mexicana.
abstract: Complex glycans derived from lipid, nucleic acid and protein free extracts of Taenia crassiceps metacestode larvae were found to have adjuvant effect against Leishmania mexicana antigens in BALB/c mice experimentally infected with L. mexicana. A single intraperitoneal or subcutaneous injection of Taenia glycans altered the Th-1/Th-2 balance in experimentally infected mice as determined by Western blot analysis of IgG 1 and IgG 2a antibodies to L. mexicana antigens. Leishmania antigens which were immunogenic in Taenia glycan vaccinated mice were different from those of non-vaccinated mice. Vaccination induced leishmania antigen specific IFN-gamma expression in vitro culture by spleen cells from Taenia glycan vaccinated-leishmania infected mice and not from mock vaccinated-leishmania infected BALB/c mice. We conclude that T. crassiceps glycans have immunoadjuvant effects against leishmania and may be developed as adjuvants in anti-leishmania vaccines.
authors.affiliation: Department of Microbiology, Faculty of Medicine, United Arab Emirates University, PO Box 17666, Al Ain, United Arab Emirates. sendiss@uaeu.ac.ae
authors.forename: Senarath
authors.initials: S
authors.lastname: Dissanayake
authors.forename: Allen
authors.initials: A
authors.lastname: Shahin
authors.forename: Abdul Majeed
authors.initials: AM
authors.lastname: Ameen
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D018946:Chemokine CCL5 / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007894:Leishmania mexicana / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000276:immunology / Q000378:metabolism; D013621:Taenia / Q000276:immunology* / Q000378:metabolism; D014612:Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D018946:Chemokine CCL5; D007074:Immunoglobulin G; D015850:Interleukin-6; D011134:Polysaccharides; D014612:Vaccines; D007371:Interferon-gamma