title: Ball lens coupled fiber-optic probe for depth-resolved spectroscopy of epithelial tissue.
abstract: A ball lens coupled fiber-optic probe design is described for depth-resolved measurements of the fluorescence and reflectance properties of epithelial tissue. A reflectance target, fluorescence targets, and a two-layer tissue phantom consisting of fluorescent microspheres suspended in collagen are used to characterize the performance of the probe. Localization of the signal to within 300 microm of the probe tip is observed by use of reflectance and fluorescence targets in air. Differential enhancement of the fluorescence signal from the top layer of the two-layer tissue phantom is observed.
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin, 1 University Station, C0800, Austin, Texas 78712, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Schwarz
authors.forename: Dizem
authors.initials: D
authors.lastname: Arifler
authors.forename: Sung K
authors.initials: SK
authors.lastname: Chang
authors.forename: Ina
authors.initials: I
authors.lastname: Pavlova
authors.forename: Insiya A
authors.initials: IA
authors.lastname: Hussain
authors.forename: Vivian
authors.initials: V
authors.lastname: Mack
authors.forename: Bob
authors.initials: B
authors.lastname: Knight
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.forename: Ann M
authors.initials: AM
authors.lastname: Gillenwater
mesh_terms: D000818:Animals; D004848:Epithelium / Q000502:physiology* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D055100:Optical Fibers; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods; D014159:Transducers