title: Intra-coronary high-dose CD34+ stem cells in patients with chronic ischemic heart disease: a 12-month follow-up.
abstract: Current stem cell protocols for ischemic heart disease are limited by the small numbers of cells that can be obtained by bone marrow aspirate. To increase myocardial delivery of bone marrow stem cells in patients with chronic ischemic heart disease (CIHD), we used granulocyte colony stimulating factor (G-CSF) for bone marrow mobilization of CD34+ cells, enabling intracoronary infusion of large numbers of CD34+ stem cells. Patients with CIHD (n = 5) demonstrated significantly reduced numbers of CD34+ cells mobilized by G-CSF in comparison to age-matched controls. Sustained reduction in anginal symptoms and improvement in quality of life scores was seen in all patients following infusion of cells. Moreover, mean collateral flow grade at 12-month follow-up angiography significantly improved, indicating sustained myocardial neovascularization. No proliferative retinopathy was induced and no in-stent restenosis seen. However, in two patients with documented increase in collateral flow, complications arose, one developing an acute coronary syndrome and the other a lentigo maligna. These results demonstrate the feasibility of G-CSF mobilization, leukapheresis and intracoronary transfer of CD34+ stem cells in patients with CIHD, but longer-term studies are required to ensure that this protocol is safe and effective.
authors.affiliation: Department of Medicine, University of Melbourne, St Vincent's Hospital, Princes St, Fitzroy 3065, Victoria, Australia. aboyle@medstv.unimelb.edu.au
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Boyle
authors.forename: Robert
authors.initials: R
authors.lastname: Whitbourn
authors.forename: Stephen
authors.initials: S
authors.lastname: Schlicht
authors.forename: Henry
authors.initials: H
authors.lastname: Krum
authors.forename: Alfred
authors.initials: A
authors.lastname: Kocher
authors.forename: Harshal
authors.initials: H
authors.lastname: Nandurkar
authors.forename: Steven
authors.initials: S
authors.lastname: Bergmann
authors.forename: Mark
authors.initials: M
authors.lastname: Daniell
authors.forename: Justin
authors.initials: J
authors.lastname: O'Day
authors.forename: Donna
authors.initials: D
authors.lastname: Skerrett
authors.forename: David
authors.initials: D
authors.lastname: Haylock
authors.forename: Richard E
authors.initials: RE
authors.lastname: Gilbert
authors.forename: Silviu
authors.initials: S
authors.lastname: Itescu
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D018952:Antigens, CD34 / Q000008:administration & dosage*; D003097:Collateral Circulation; D017023:Coronary Angiography; D005260:Female; D005500:Follow-Up Studies; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000628:therapy*; D011788:Quality of Life; D015899:Tomography, Emission-Computed, Single-Photon; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor