Assessment of multiple displacement amplification for polymorphism discovery and haplotype determination at a highly polymorphic locus, MC1R.
The identification of common genetic variants such as single nucleotide polymorphisms (SNPs) in the human genome has become central in human population genetics and evolution studies, as well as in the study of the genetic basis of complex traits and diseases. Crucial for the accurate identification of genetic variants is the availability of high quality genomic DNA (gDNA). Since popular sources of gDNA (buccal cells, lymphocytes, hair bulb) often do not yield sufficient quantities of DNA for molecular genetic applications, whole genome amplification methods have recently been introduced to generate a renewable source of double-stranded linear DNA. Here, we assess the fidelity of one method, multiple displacement amplification (MDA), which utilizes bacteriophage Phi29 DNA polymerase to generate amplified DNA from an original source of gDNA, in a representative SNP discovery and genetic association study at the melanocortin 1 receptor (MC1R) locus, a highly polymorphic gene in humans involved in skin and hair pigmentation. We observed that MDA has high fidelity for novel SNP discovery and can be a valuable tool in generating a potentially indefinite source of DNA. However, we observed an allele amplification bias that causes genotype miscalls at heterozygous sites. At loci with multiple polymorphic sites in linkage disequilibrium, such as at MC1R, this bias can create a significant number of heterozygote genotype errors that subsequently misrepresents haplotypes.
Murthy
Kenton K
KK
Polymorphism Research Laboratory, Department of Psychiatry, University of California at San Diego, La Jolla 92093, USA.
Mahboubi
Vafa S
VS
Santiago
Alicia
A
Barragan
Mandy T
MT
Knöll
Ralph
R
Schultheiss
Heinz-Peter
HP
O'Connor
Daniel T
DT
Schork
Nicholas J
NJ
Rana
Brinda K
BK
D000483:Alleles; D017103:Bacillus Phages / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics* / Q000502:physiology*
D044102:Receptor, Melanocortin, Type 1; D004247:DNA; D004259:DNA-Directed DNA Polymerase