title: Programming of glucose-insulin metabolism in adult sheep after maternal undernutrition.
abstract: The present study examines the effects of late vs. early gestation undernutrition on adult glucose-insulin homeostasis in sheep and investigates whether the lower birth weight of twins alters glucose-insulin handling in adult life. Pregnant sheep were fed to requirement (100% intake) from day 0 of gestation to term [ approximately 147 days of gestation (dGA), control singles (CS) n = 5; control twins (CT) n = 5] or to 50% requirement from days 0-30 dGA [nutrient restricted during early gestation (NRE); n = 5] or day 110-term [NR during late nutrition (NRL); n = 4]. At all other times, NR sheep received 100% intake. All sheep lambed naturally; offspring were weaned at 10 wk and were reared on pasture until 1 yr of age. At this time, indwelling catheters were inserted, and 2-4 days later, basal metabolic and endocrine status and responses to an intravenous glucose tolerance test (IVGTT) and feeding were assessed. Adipose and skeletal muscle were then sampled after humane euthanasia and were analyzed for expression of insulin-signaling proteins and GLUT4. Between groups, birth weight of singletons was similar and increased relative to twins. At 1 yr of age, weights were similar between groups. The areas under the curve for glucose and insulin during the IVGTT were greater in NRL vs. other groups, indicating glucose intolerance. This was associated with reduced adipose, but not muscle, GLUT4, and increased adipose tissue mass. Adult glucose-insulin homeostasis in sheep was unaffected by fetal number. In conclusion, prenatal undernutrition, specifically during late gestation, affects adult offspring intermediary metabolism, and, in particular, glucose-insulin homeostasis.
authors.affiliation: School of Human Development, Academic Division of Child Health, University Hospital, Nottingham, NG7 2UH, UK. david.gardner@nottingham.ac.uk
authors.forename: D S
authors.initials: DS
authors.lastname: Gardner
authors.forename: K
authors.initials: K
authors.lastname: Tingey
authors.forename: B W M
authors.initials: BW
authors.lastname: Van Bon
authors.forename: S E
authors.initials: SE
authors.lastname: Ozanne
authors.forename: V
authors.initials: V
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Dandrea
authors.forename: D H
authors.initials: DH
authors.lastname: Keisler
authors.forename: T
authors.initials: T
authors.lastname: Stephenson
authors.forename: M E
authors.initials: ME
authors.lastname: Symonds
mesh_terms: D000818:Animals; D001724:Birth Weight; D001823:Body Composition; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D044342:Malnutrition / Q000378:metabolism*; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena; D012756:Sheep; D014427:Twins / Q000378:metabolism
chemical_list: D007328:Insulin; D005947:Glucose