title: Serum surfactant protein D is increased in acute and chronic inflammation in mice.
abstract: Surfactant protein A (SP-A) and surfactant protein D (SP-D) are important components of innate immunity that can modify the inflammatory response. However, alterations and regulation of SP-A and SP-D in acute and chronic inflammation are not well defined. In addition, serum SP-D may serve as a biomarker of lung inflammation. We determined the expression of SP-A and SP-D in murine models. To study acute inflammation, we instilled bleomycin intrabronchially. To study chronic lung inflammation, we used a transgenic mouse that overexpresses tumor necrosis factor (TNF)-alpha under the control of the SP-C promoter. These mice have a chronic mononuclear cell infiltration, airspace enlargement, pulmonary hypertension, and focal pulmonary fibrosis. In acute inflammation model, levels of mRNA for all surfactant proteins were reduced after bleomycin administration. However, serum SP-D was increased from days 7 to 28 after instillation. In chronic inflammation model, SP-D mRNA expression was increased, whereas the expression of SP-A, SP-B and SP-C was reduced. Both serum and lung SP-D concentrations were increased in chronic lung inflammation. These data clarified profile of SP-A and SP-D in acute and chronic inflammation and indicated that serum SP-D can serve as a biomarker of lung inflammation in both acute and chronic lung injury in mice.
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Maidashi, Fukuoka, Japan. fujita@kokyo.med.kyushu-u.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Fujita
authors.forename: John M
authors.initials: JM
authors.lastname: Shannon
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ouchi
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Voelker
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Mason
mesh_terms: D000208:Acute Disease; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid; D002908:Chronic Disease; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D011014:Pneumonia / Q000097:blood* / Q000139:chemically induced / Q000473:pathology*; D037663:Pulmonary Surfactant-Associated Protein D / Q000097:blood* / Q000235:genetics; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014598:Uteroglobin / Q000235:genetics
chemical_list: D037663:Pulmonary Surfactant-Associated Protein D; C556390:Scgb1a1 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D001761:Bleomycin; D014598:Uteroglobin