[PCR as an alternative method for detecting toxigenic strains of Corynebacterium diphtheriae].
PCR standardization was performed in order to detect a fragment and a whole tox gene which is presented in all toxigenic strains of Corynebacterium diphtheriae. PCR is one of the least time-consuming methods for detection toxigenicity of C. diphtheriae strains near EIA (enzyme immunoassay) and ICS (immunochromatographic strip) tests.
Zasada
Aleksandra Anna
AA
Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie. azasada@pzh.gov.pl
D002845:Chromatography / Q000379:methods; D003353:Corynebacterium diphtheriae / Q000302:isolation & purification*; D004165:Diphtheria / Q000382:microbiology*; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D004167:Diphtheria Toxin