title: Development of a cost-effective oral vaccination method against viral disease in fish.
abstract: Different vaccination methods have been applied to protect fish against the detrimental effects of various pathogens. Several studies have shown the potentials of oral vaccination. In theory oral vaccination is an effortless and stress-free method which can be applied at almost any age. In general, however, the vaccine has to be protected to avoid digestion, which results in high costs for application in aquaculture. In this paper we introduce a cost-effective oral vaccination strategy for viral diseases of fish. The vaccines discussed here include fusion proteins consisting of a gut adhesion molecule and a viral peptide expressed in plants. The adhesion molecule mediates binding to and uptake from the gut, whereas the viral peptide functions as vaccine antigen mediating the induction of a humoral immune response. The first pilot studies using a fusion of the gut adhesion molecule and well-characterised heterologous linear B- and T-cell viral epitopes, produced in potato tubers, showed a promising binding and subsequent uptake in the end gut of carp. The results further indicated that a specific humoral immune response was evoked.
authors.affiliation: Wageningen University and Research Centre: Department of Animal Sciences, Cell Biology and Immunology Group, The Netherlands. arjen.companjen@wur.nl
authors.forename: A R
authors.initials: AR
authors.lastname: Companjen
authors.forename: D E A
authors.initials: DE
authors.lastname: Florack
authors.forename: J H M W
authors.initials: JH
authors.lastname: Bastiaans
authors.forename: C I
authors.initials: CI
authors.lastname: Matos
authors.forename: D
authors.initials: D
authors.lastname: Bosch
authors.forename: J H W M
authors.initials: JH
authors.lastname: Rombout
mesh_terms: D000284:Administration, Oral; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001427:Bacterial Toxins / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D002347:Carps; D004768:Enterotoxins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D005393:Fish Diseases / Q000517:prevention & control*; D006652:Histological Techniques / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D011198:Solanum tuberosum / Q000378:metabolism / Q000821:virology; D014611:Vaccination / Q000379:methods* / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000378:metabolism; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000378:metabolism; D014777:Virus Diseases / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018984:Epitopes, T-Lymphocyte; D029968:Escherichia coli Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C027538:heat-labile enterotoxin, E coli