[Effects of Triton X-100 on the oxygen uptake rate of photosystem I particles treated at 70 degrees C].
The characteristics including oxygen uptake rates, fluorescence spectra and absorption spectra of photosystem I particles with or without Triton-X 100 treatment before or after the incubation at 70 degrees C for 10 min were compared. The oxygen uptake rates of photosystem I particles decreased after being incubated at 70 degrees C for 10 min, which could be recovered by the addition of Triton-X 100. Singlet oxygen was formed when the light-harvesting complex I was separated from the core complex of photosystem I, which resulted in high oxygen uptake rate. There was much difference in the fluorescence spectra of photosystem I particles between photosystem I particles treated with Triton-X 100 after the incubation at 70 degrees C for 10 min or not, which implies the ability of Triton-X 100 to promote the recovery of photosystem I particles after the incubation at 70 degrees C for 10 min.
Chen
Wei
W
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Yang
Zhen-Le
ZL
Li
Liang-Bi
LB
Kuang
Ting-Yun
TY
D006358:Hot Temperature; D017830:Octoxynol / Q000494:pharmacology*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000737:chemistry / Q000378:metabolism*; D026082:Singlet Oxygen / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D045331:Photosystem I Protein Complex; D026082:Singlet Oxygen; D017830:Octoxynol; D010100:Oxygen