Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus.
Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field.
Prins
Marcel
M
Laboratory of Virology, Wageningen University, The Netherlands.
Lohuis
Dick
D
Laboratory of Virology, Wageningen University, The Netherlands.
Schots
Arjen
A
Laboratory of Molecular Recognition and Antibody Technology, Wageningen University, The Netherlands.
Goldbach
Rob
R
Laboratory of Virology, Wageningen University, The Netherlands.
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D019151:Peptide Library; D010935:Plant Diseases / Q000821:virology; D030821:Plants, Genetically Modified / Q000276:immunology*; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017865:Tospovirus / Q000276:immunology / Q000472:pathogenicity*
D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D019151:Peptide Library