[Osteogenic differentiation of synovial mesenchymal stem cells in vitro].
OBJECTIVE
To investigate the potential of synovial mesenchymal stem cells (SMSC) in osteogenic differentiation.


METHODS
SMSC were obtained by limited dilution method and expanded to culture in 25-milliliter flasks. The attached cells were treated with inductive medium containing dexamethasone, glycerophosphate and vitamin C at 3rd passage SMSC. The mineralized nodule was stained by Von Kossa method. The expression of ALP and osteopontin were detected by histochemical, immunohistological staining technique, respectively, while the expression of cbfa1 mRNA by RT-PCR.


RESULTS
Pure SMSC which were of spindle shape and star shape, uniform in size, could be induced to pleomorphism osteoblast in vitro, which were intensive positive in ALP and osteopontin. The expression of cbfa1 mRNA were also verified by RT-PCR and the polygonal cells formed nodular structure at 4 weeks. All these were coincident with the characters of osteoblast.


CONCLUSIONS
SMSC can be purified and induced into osteoblast in vitro.
Li
Jian
J
Dept. of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Long
Xing
X
Zhu
Fan
F
Yang
Xue-Chao
XC
D001205:Ascorbic Acid; D002454:Cell Differentiation; D003907:Dexamethasone; D005994:Glycerophosphates; D006801:Humans; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D005994:Glycerophosphates; D012333:RNA, Messenger; D053495:Osteopontin; D003907:Dexamethasone; D001205:Ascorbic Acid