[Influence of wall polymer and preparation process on the particle size and encapsulation of hemoglobin microcapsules].
Methoxypoly (ethylene glycol)- block-poly (DL-lactide) (PELA) microcapsules containing bovine hemoglobin (BHb) were prepared by a W/O/W double emulsion-solvent diffusion process. The P50 and Hill coeffcient were 3466 Pa and 2.4 respectively, which were near to the natural bioactivity of bovine hemoglobin. The results suggested that polymer composition had significant influence on encapsulation efficiency and particle size of microcapsules. The encapsulation efficiency could reach 90% and the particle size 3 - 5 microm when the PELA copolymer containing MPEG 2000 block was used. The encapsulation efficiency and particle size increased with the concentration of PELA. Increasing the concentrations of NaCl in outer aqueous solution resulted in the increase of encapsulation efficiency and the decrease of particle size. As the concentration of stabilizer in outer aqueous solution increased in the range of 10 g/L to 20 g/L, the particle size reduced while encapsulation efficiency was increased, further increase of the stabilizer concentration would decrease encapsulation efficiency. Increasing of primary emulsion stirring rate was advantageous to the improvement of encapsulation efficiency though it had little influence on the particle size. The influence of re-emulsion stirring rate was complicated, which was not apparent in the case of large volume of re-emulsion solution. When the wall polymer and primary emulsion stirring rate were fixed, the encapsulation efficiency decreased as the particle size reduced.
Qiu
Wei
W
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, the Chinese Academy of Sciences, PO Box 353, Beijing 100080, China.
Ma
Guang-Hui
GH
Meng
Fan-Tao
FT
Su
Zhi-Guo
ZG
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002214:Capsules; D002417:Cattle; D006454:Hemoglobins / Q000378:metabolism*; D007773:Lactates / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D001672:Biocompatible Materials; D002214:Capsules; D006454:Hemoglobins; D007773:Lactates; C058523:polyethylene oxide-polylactic acid block copolymer; D011092:Polyethylene Glycols