title: Circular dichroism spectroscopy for the study of protein-ligand interactions.
abstract: Circular dichroism (CD) is the difference in absorption of left and right circularly polarized light, usually by a solution containing the molecules of interest. A signal is only measured for chiral molecules such as proteins. A CD spectrum provides information about the bonds and structures responsible for this chirality. When a small molecule (or ligand) binds to a protein, it acquires an induced CD (ICD) spectrum through chiral perturbation to its structure or electron rearrangements. The wavelengths of this ICD are determined by the ligand's own absorption spectrum, and the intensity of the ICD spectrum is determined by the strength and geometry of its interaction with the protein. Thus, ICD can be used to probe the binding of ligands to proteins. This chapter outlines protein CD and ICD, together with some of the issues relating to experimental design and implementation.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Rodger
authors.forename: Rachel
authors.initials: R
authors.lastname: Marrington
authors.forename: David
authors.initials: D
authors.lastname: Roper
authors.forename: Stuart
authors.initials: S
authors.lastname: Windsor
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000199:Actins; D008024:Ligands; D010455:Peptides; D011506:Proteins; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase