[Correlations of expressions of Glut1 and HIF-1alpha to cellular proliferation of colorectal adenocarcinoma].
OBJECTIVE
Malignant cells exhibit increased glucose metabolism and microenvironment hypoxia. Glucose transporter-1 (Glut1) and hypoxia-inducible factor 1 alpha (HIF-1alpha) enhance glucose metabolism under hypoxia. This study was to investigate the expressions of Glut1 and HIF-1alpha in colorectal adenocarcinoma, and explore their correlations to cell proliferation.


METHODS
Immunohistochemistry was used to detect expressions of Glut1, HIF-1alpha, and proliferating cell nuclear antigen (PCNA) in 60 specimens of colorectal adenocarcinoma and 20 specimens of normal colorectal tissues. Their interrelations were analyzed.


RESULTS
Positive rates of Glut1 and HIF-1alpha were significantly higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (58.3% vs. 0, P < 0.01; 73.3% vs. 0, P < 0.01). PCNA level was significantly higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (65.25+/-16.35 vs. 15.20+/-3.47, P < 0.01). Glut1 and HIF-1alpha levels were positively correlated with PCNA level (r(1)=0.409, P < 0.01; r(2)=0.323, P < 0.05), and were associated with Dukes' stage and lymph node metastasis.


CONCLUSIONS
The overexpressions of Glut1 and HIF-1alpha play important roles in carcinogenesis and progression of colorectal adenocarcinoma, and closely correlate with cell proliferation of colorectal adenocarcinoma.
Zhou
Yu-Ling
YL
Department of Gastroenterology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P. R. China.
Deng
Chang-Sheng
CS
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D051272:Glucose Transporter Type 1; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018809:Proliferating Cell Nuclear Antigen