title: PAX5/IGH rearrangement is a recurrent finding in a subset of aggressive B-NHL with complex chromosomal rearrangements.
abstract: We present an extensive characterization of 10 B-cell lymphomas with a t(9;14)(p13;q32). The presence of the PAX5/IGH gene rearrangement was demonstrated by fluorescence in situ hybridization (FISH) using a validated probe set, whereas complex karyotypic changes were reassessed by multiplex-FISH (M-FISH). Pathologic and clinical review revealed the presence of this rearrangement in 4 histiocyte-rich, T-cell-rich B-cell lymphomas (HRTR-BCLs) and 2 posttransplantation diffuse large B-cell lymphomas (PTLD-DLBCLs). In contrast to initial observations describing this translocation in lymphoplasmacytic lymphoma (LPL) and LPL-derived large B-cell lymphoma, our data showed a wide morphologic and clinical spectrum associated with the PAX5/IGH rearrangement, pointing to an association between this aberration and a subset of de novo DLBCLs presenting with advanced disease and adverse prognosis. In addition, the recurrent incidence of this rearrangement in both HRTR-BCL (4 cases) and PTLD-DLBCL (2 cases) was previously unrecognized and is intriguing.
authors.affiliation: Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
authors.forename: Bruce
authors.initials: B
authors.lastname: Poppe
authors.forename: Pascale
authors.initials: P
authors.lastname: De Paepe
authors.forename: Lucienne
authors.initials: L
authors.lastname: Michaux
authors.forename: Nicole
authors.initials: N
authors.lastname: Dastugue
authors.forename: Christian
authors.initials: C
authors.lastname: Bastard
authors.forename: Christian
authors.initials: C
authors.lastname: Herens
authors.forename: Els
authors.initials: E
authors.lastname: Moreau
authors.forename: Francesco
authors.initials: F
authors.lastname: Cavazzini
authors.forename: Nurten
authors.initials: N
authors.lastname: Yigit
authors.forename: Heidi
authors.initials: H
authors.lastname: Van Limbergen
authors.forename: Anne
authors.initials: A
authors.lastname: De Paepe
authors.forename: Marleen
authors.initials: M
authors.lastname: Praet
authors.forename: Chris
authors.initials: C
authors.lastname: De Wolf-Peeters
authors.forename: Iwona
authors.initials: I
authors.lastname: Wlodarska
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002883:Chromosomes, Human, Pair 14; D002899:Chromosomes, Human, Pair 9; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051757:PAX5 Transcription Factor; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D014157:Transcription Factors