Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique.
At pH 9.75, the resonance light scattering (RLS) intensity of OA-Eu3+ system is greatly enhanced by nucleic acid. Based on this phenomenon, a new quantitative method for nucleic acid in aqueous solution has been developed. Under the optimum condition, the enhanced RLS is proportional to the concentration of nucleic acid in the range of 1.0x10(-9) to 1.0x10(-6)g/ml for herring sperm DNA, 8.0x10(-10) to 1.0x10(-6) g/ml for calf thymus DNA and 1.0x10(-9) to 1.0x10(-6) g/ml for yeast RNA, and their detection limits are 0.020, 0.011 and 0.010 ng/ml, respectively. Synthetic samples and actual samples were satisfactorily determined. In addition, the interaction mechanism between nucleic acid and OA-Eu3+ is also investigated.
Wu
Xia
X
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, PR China.
Sun
Shuna
S
Yang
Jinghe
J
Wang
Minqin
M
Liu
Liyan
L
Guo
Changying
C
D004247:DNA / Q000032:analysis; D005063:Europium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D010093:Oxolinic Acid / Q000737:chemistry*; D010710:Phosphates / Q000494:pharmacology; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D013997:Time Factors
D015335:Molecular Probes; D009696:Nucleic Acids; D010710:Phosphates; D005063:Europium; D004247:DNA; C427203:calf thymus DNA; D010093:Oxolinic Acid