Increased density of ghrelin-expressing cells in the gastric fundus and body in Prader-Willi syndrome.
BACKGROUND
The levels of ghrelin, an orexigenic hormone secreted by oxyntic cells in the digestive tract, are elevated in Prader-Willi syndrome (PWS) and GH deficiency (GHD) patients. In this study, we hypothesized that the hyperghrelinemia observed in PWS is related to IGF-I or GH/IGF axis deficiency.


METHODS
We investigated the densities of ghrelin-expressing cells (GECs), the amounts of ghrelin in gastric tissues, and ghrelin levels in plasma in 16 PWS patients and compared these results with those of 13 GHD patients and comparison groups (19 normal lean and 10 normal obese subjects).


RESULTS
In the gastric body and fundus, 2- to 3-fold increases in the numbers of GECs (P < 0.001) and in the amounts of ghrelin (P < 0.018) were noted in PWS patients vs. comparison groups, whereas GEC numbers in GHD patients were similar to those of the comparison group despite elevated fasting plasma ghrelin levels. In addition, IGF-I sd scores in PWS were not found to be correlated with GEC densities, the amounts of ghrelin expressed in gastric tissues, or plasma ghrelin levels.


CONCLUSIONS
Our results suggest that IGF-I or GH/IGF axis deficiency appears to be unrelated to observed GEC increases in the stomach of patients with PWS.
Choe
Yon Ho
YH
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Il-Won Dong, Gang-Nam Gu, Seoul, 135-710, Korea.
Song
Sang Yong
SY
Paik
Kyung-Hoon
KH
Oh
Yoo Joung
YJ
Chu
Su-Hyun
SH
Yeo
Sung Hee
SH
Kwon
Eun Kyung
EK
Kim
Eun Mee
EM
Rha
Mee Yong
MY
Jin
Dong-Kyu
DK
D016022:Case-Control Studies; D002452:Cell Count; D002648:Child; D005260:Female; D005748:Gastric Fundus / Q000378:metabolism* / Q000473:pathology*; D005753:Gastric Mucosa / Q000378:metabolism*; D054439:Ghrelin; D019382:Human Growth Hormone / Q000172:deficiency; D006801:Humans; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D036361:Peptide Hormones / Q000097:blood / Q000378:metabolism*; D011218:Prader-Willi Syndrome / Q000378:metabolism* / Q000473:pathology*; D043202:Steroid Metabolism, Inborn Errors / Q000378:metabolism / Q000473:pathology; D013270:Stomach / Q000473:pathology*; D014018:Tissue Distribution
D054439:Ghrelin; D036361:Peptide Hormones; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I