title: [Frequency of prothrombin gene G20210A variant in the 3'-untranslated region in Zhuang ethnic Chinese].
abstract: OBJECTIVE
  To ascertain the frequency of prothrombin (FII) gene 3'-untranslated region (3'-UT) G20210A variant and to explore whether this mutation is related to arterial thrombosis in Chinese Zhuang population.
  
  
  METHODS
  Seventy-six patients with cerebral thrombosis, 23 patients with myocardial infarction and 106 healthy Chinese Zhuang persons were studied. The G20210A mutant allele of the prothrombin gene in all blood specimens was investigated by DNA extraction, polymerase chain reaction amplification, Hind III digestion and polyacrylamide gel electrophoresis.
  
  
  RESULTS
  The patients and normal control subjects were all homozygous for the normal G20210G allele, and there was no FII G20210A variant.
  
  
  CONCLUSIONS
  Factor II gene 3'-UT G20210A mutant allele is absent in the 99 Chinese Zhuang ethnic patients with ischemic stroke and myocardial infarction and is absent in 106 normal healthy Zhuang people. FII G20210A mutation may not be a major risk factor for thrombogenesis in ethnic Chinese.
authors.affiliation: Department of Medical Oncology, Cancer Center of Sun Yat-sen University, Guangzhou, Guangdong, 510060, PR China. changjianhua@163.com
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Chang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.forename: Guang-sen
authors.initials: GS
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002681:China; D004252:DNA Mutational Analysis; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D020767:Intracranial Thrombosis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009203:Myocardial Infarction / Q000235:genetics; D016133:Polymerase Chain Reaction; D011516:Prothrombin / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D011516:Prothrombin