title: Expression and function of syndecan-4 in human platelets.
abstract: Platelet recruitment crucially depends on amplification systems provided by autocrine and paracrine factors such as adenosine diphosphate. In inflammatory states, consumption of coagulation proteins, such as antithrombin aggravates the procoagulant state. In this study, we report that platelets express syndecan-4, an antithrombin-binding cell surface heparan sulphate proteoglycan, whose ligation with antithrombin inhibits activated platelet-dependent superoxide anion release from neutrophils by the limitation of adenosine diphosphate and adenosine triphosphate secretion in activated platelets. Adenosine triphosphate-induced platelet aggregation is reduced after treatment of platelets with antithrombin, which is reversed by blockade of syndecan-4. We further observed that antithrombin limits CD40 ligand expression in adenosine diphosphate-activated platelets and inhibits the shedding of syndecan-4 from activated platelets. Syndecan-4 appears to be directly involved in regulating platelet aggregation as anti-syndecan-4 antibody augments platelet aggregation. We suggest that antithrombin might exert beneficial effects in disseminated intravascular coagulation by reducing platelet activation, observed as inhibited CD40 ligand expression, syndecan-4 shedding, and adenosine diphosphate- and adenosine triphosphate-release from activated platelets with subsequent inhibition of neutrophil respiratory burst. From these data it is concluded that syndecan-4 may play important roles in the regulation of inflammatory effects of platelets.
authors.affiliation: Department of Internal Medicine, University of Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Kaneider
authors.forename: Clemens
authors.initials: C
authors.lastname: Feistritzer
authors.forename: Donatella
authors.initials: D
authors.lastname: Gritti
authors.forename: Birgit A
authors.initials: BA
authors.lastname: Mosheimer
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ricevuti
authors.forename: Josef R
authors.initials: JR
authors.lastname: Patsch
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Wiedermann
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D000991:Antithrombins / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D023201:CD40 Ligand / Q000097:blood; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000097:blood; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics; D009504:Neutrophils / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects; D011509:Proteoglycans / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D016897:Respiratory Burst; D015398:Signal Transduction; D053671:Syndecan-4
chemical_list: D000991:Antithrombins; D018836:Inflammation Mediators; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; C508304:SDC4 protein, human; D053671:Syndecan-4; D023201:CD40 Ligand; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate