Update on reproductive biotechnologies in small ruminants and camelids.
Recent advances in reproductive biotechnologies in small ruminants include improvement of methods for in vitro production of embryos and attempts at spermatogonial stem cell transplantation. In vitro production of embryos by IVM/IVF, intra-cytoplasmic sperm injection (ICSI), or nuclear transfer (NT) has been made possible by improvements in oocyte collection and maturation techniques, and early embryo culture systems. However, in vitro embryo production still is not very efficient due to several limiting factors affecting the outcome of each step of the process. This paper discusses factors affecting in vitro embryo production in small ruminants and camelids, as well as preliminary results with the technique of spermatogonial stem cell transplantation.
Tibary
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Department of Veterinary Clinical Sciences and Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6610, USA. tibary@vetmed.wsu.edu
Anouassi
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Khatir
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D000818:Animals; D002161:Camelids, New World; D002478:Cells, Cultured; D019976:Cloning, Organism; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D018587:Oocyte Donation / Q000662:veterinary; D009865:Oocytes / Q000502:physiology; D027724:Reproductive Techniques, Assisted / Q000639:trends / Q000662:veterinary*; D012418:Ruminants; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary; D013094:Spermatozoa; D033581:Stem Cell Transplantation / Q000662:veterinary; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary