Direct measurement of gastric H+/K+-ATPase activities in patients with or without Helicobacter pylori-associated chronic gastritis.
OBJECTIVE
The role of Helicobacter pylori (H pylori) infection in gastric acid secretion of patients with chronic gastritis remains controversial. This study was designed to elucidate the effect of H pylori on H+/K+-ATPase activities in gastric biopsy specimens.


METHODS
Eighty-two patients with chronic gastritis who had undergone upper endoscopy were included in this study. H pylori infection was confirmed by rapid urease test and histology. Gastric H+/K+-ATPase activities and serum gastrin concentrations were measured by an enzymatic method and radioimmunoassay, respectively. For those patients who received triple therapy for eradicating H pylori, changes in the activity of gastric H+/K+-ATPase and serum gastrin levels were also measured.


RESULTS
The mean gastric H+/K+-ATPase activity in H pylori-positive group (42 patients) was slightly higher than that in H pylori-negative group (29 patients) (169.65+/-52.9 and 161.38+/-43.85 nmol Pi/(mg.h), respectively, P=0.301). After eradication of H pylori, the gastric H+/K+-ATPase activities slightly decreased compared to prior therapy (165.03+/-59.50 and 158.42+/-38.93 nmol Pi/(mg.h), respectively, P=0.805). The mean basal gastrin concentration was slightly higher in H pylori-positive patients than in H pylori-negative patients (87.92+/-39.65 pg/mL vs 75.04+/-42.57 pg/mL, P=0.228). The gastrin levels fell significantly after the eradication of H pylori. (Before treatment 87.00+/-30.78 pg/mL, after treatment 64.73+/-18.96 pg/mL, P=0.015).


CONCLUSIONS
Gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronic gastritis.
Thong-Ngam
Duangporn
D
Department of Physiology, Faculty of Medicine, Chulalongkorn University Hospital, Bangkok 10330, Thailand. d.thong@mailcity.com
Tangkijvanich
Pisit
P
Sampatanukul
Pichet
P
Prichakas
Paungpayom
P
Mahachai
Varocha
V
Tosukowong
Piyaratana
P
D002908:Chronic Disease; D005753:Gastric Mucosa / Q000201:enzymology*; D005755:Gastrins / Q000097:blood; D005756:Gastritis / Q000201:enzymology / Q000382:microbiology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000201:enzymology*; D016480:Helicobacter pylori; D006801:Humans; D012016:Reference Values
D005755:Gastrins; D017506:H(+)-K(+)-Exchanging ATPase