title: Regulation by allergens of chemokine receptor expression on in vitro-generated dendritic cells.
abstract: Immature dendritic cells (DCs) derived from CD34+ progenitor cells or peripheral monocytes, are used as in vitro sensitization models in many chemical allergen treatment studies. During the sensitization, DCs follow maturation process and gain the capacity to migrate to lymph nodes where they stimulate T cells. Chemokine receptor allows DCs to migrate along chemotactic gradients. In this work, we used immature DCs from peripheral monocytes to evaluate the influence of allergens on chemokine receptor and surface-marker expression. We tested the sensitizers dinitrochlorobenzene, Bandrowski's base, and coumarin, as well as the tolerogen dichloronitrobenzene, the irritant sodium dodecyl sulfate and the solvent dimethyl sulfoxide. All skin sensitizers up-regulated the co-stimulatory molecule CD86 and increased the CD83+ cell population. No expression of the chemokine receptors CCR2, CCR3, CCR6, or CXCR5 was observed on DCs exposed to the tested chemicals. The strong allergen dinitrochlorobenzene slightly increased CCR7 expression on DCs but down-regulated CCR1 surface expression. CCR1 down-regulation was not mediated by a classical maturation pathway, as it was unaffected by the corticosteroid dexamethasone.
authors.affiliation: Laboratoire d'Immunologie, GURIFA, Faculté de Médecine, Université de Rennes I, 35043 Rennes, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Jugdé
authors.forename: Christine
authors.initials: C
authors.lastname: Boissier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rougier-Larzat
authors.forename: Anne
authors.initials: A
authors.lastname: Corlu
authors.forename: Christophe
authors.initials: C
authors.lastname: Chesné
authors.forename: Gilbert
authors.initials: G
authors.lastname: Semana
authors.forename: Denis
authors.initials: D
authors.lastname: Heresbach
mesh_terms: D000485:Allergens / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen; D003374:Coumarins / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D066298:In Vitro Techniques; D007509:Irritants / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008562:Membrane Glycoproteins / Q000276:immunology; D010655:Phenylenediamines / Q000494:pharmacology; D019707:Receptors, Chemokine / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D000097802:CD83 Antigen
chemical_list: D000485:Allergens; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D003374:Coumarins; D004121:Dimethyl Sulfoxide; D004137:Dinitrochlorobenzene; D007136:Immunoglobulins; D007509:Irritants; D008562:Membrane Glycoproteins; D010655:Phenylenediamines; D019707:Receptors, Chemokine; D012967:Sodium Dodecyl Sulfate; D000097802:CD83 Antigen; C497964:CD86 protein, human; C051597:Bandrowski's base; C030123:coumarin