title: GPI-specific phospholipase D (GPI-PLD) is expressed during mouse development and is localized to the extracellular matrix of the developing mouse skeleton.
abstract: Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is abundant in serum and has a well-characterized biochemistry; however, its physiological role is completely unknown. Previous investigations into GPI-PLD have focused on the adult animal or on in vitro systems and a putative role in development has been neither proposed nor investigated. We describe the first evidence of GPI-PLD expression during mouse embryonic ossification. GPI-PLD expression was detected predominantly at sites of skeletal development, increasing during the course of gestation. GPI-PLD was observed during both intramembraneous and endochondral ossification and localized predominantly to the extracellular matrix of chondrocytes and to primary trabeculae of the skeleton. In addition, the mouse chondrocyte cell line ATDC5 expressed GPI-PLD after experimental induction of differentiation. These results implicate GPI-PLD in the process of bone formation during mouse embryogenesis.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Bern, Bühlstrasse 28, 3012 Bern, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Gregory
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kraemer
authors.forename: Gisela
authors.initials: G
authors.lastname: Zürcher
authors.forename: René
authors.initials: R
authors.lastname: Gentinetta
authors.forename: Valeria
authors.initials: V
authors.lastname: Rohrbach
authors.forename: Urs
authors.initials: U
authors.lastname: Brodbeck
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Andres
authors.forename: Andrew
authors.initials: A
authors.lastname: Ziemiecki
authors.forename: Peter
authors.initials: P
authors.lastname: Bütikofer
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D010739:Phospholipase D / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010739:Phospholipase D; C065017:glycoprotein phospholipase D