Fibroblast growth factor-23 mutants causing familial tumoral calcinosis are differentially processed.
Familial tumoral calcinosis (TC, OMIM 211900) is a heritable disorder characterized by hyperphosphatemia, normal or elevated serum 1,25-dihydroxyvitamin D, and often severe ectopic calcifications. Two recessive mutations in fibroblast growth factor-23 (FGF23), serine 71/glycine (S71G) and serine 129/phenylalanine (S129F), were identified as causing TC. Herein, we undertook comprehensive biochemical analyses of an extended TC family carrying the S71G FGF23 mutation, which revealed that heterozygous (serine/glycine, S/G) individuals had elevated serum FGF23 C-terminal fragments compared with wild-type (serine/serine, S/S) family members (P < 0.025). To understand the differential processing of FGF23 in TC patients, we transiently expressed S71G as well as S129F FGF23. FGF23 ELISA in tandem with Western analyses revealed increased proteolytic cleavage of mutant FGF23 and a limited secretion of intact protein. Furthermore, S71G and S129F FGF23 carrying mutations that disrupt the furin-like protease RXXR motif in FGF23 rescued the secretion of the intact protein, and both TC mutant proteins harboring the R176Q mutation revealed no altered sensitivity to trypsin compared with the native (R176Q)FGF23. Finally, S71G, but not S129F mutant FGF23, is rescued by temperature. In summary, FGF23 mutations causing TC lead to increased intracellular proteolysis of FGF23, most likely by furin-like proteases, due to conformational changes of the mutant protein. The destabilizing nature of these mutations provides new insight into the pathophysiology of TC and exemplifies the physiological importance of FGF23 in phosphate and vitamin D metabolism.
Larsson
Tobias
T
Department of Medical and Molecular Genetics, 975 West Walnut Street, IB130, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
Davis
Siobhan I
SI
Garringer
Holly J
HJ
Mooney
Sean D
SD
Draman
Mohamad S
MS
Cullen
Michael J
MJ
White
Kenneth E
KE
D002114:Calcinosis / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D007668:Kidney / Q000166:cytology; D008297:Male; D008958:Models, Molecular; D010375:Pedigree; D010641:Phenotype; D017354:Point Mutation; D017433:Protein Structure, Secondary; D013696:Temperature
D018076:DNA, Complementary; C000717427:FGF23 protein, human; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23