[Biological effects of nano-hydroxyapatite/polyamide 66 on the osteoblast].
OBJECTIVE
To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.


METHODS
MTT assay was used to determine the growth of osteoblast, enzymatic measure was used to determine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in osteoblasts treated by DMEM eluate of nHA-PA66.


RESULTS
Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98% - 106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P > 0.05).


CONCLUSIONS
nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compatibility is proved.
Ye
Ling
L
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Su
Qin
Q
Zhou
Xue-Dong
XD
D017886:Durapatite / Q000494:pharmacology*; D009757:Nylons / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D009757:Nylons; D012333:RNA, Messenger; D015675:Osteocalcin; D017886:Durapatite