title: Targeted proteomics using immunoblotting technique for studying dysregulation of ion transporters in renal disorders.
abstract: Renal salt and water transport physiology has benefited tremendously from the rapid advance of proteomics. Proteomics developed as a fast-throughput means of screening for global changes in proteins in a selected tissue, organ or cell type, as a logical offshoot of similar comprehensive, messenger RNA array-type technology. Targeted proteomics utilizes similar techniques but examines a predetermined set of proteins. One approach that has been rigorously employed over the last 10 years to evaluate differences in renal protein abundances due to a treatment or genotype has been parallel semiquantitative immunoblotting using antibody arrays. This approach, and newer ones on the horizon, provide a rapid global overview of regulation of the individual proteins whose integrated action determines overall renal sodium or water reabsorption.
authors.affiliation: Department of Medicine, Georgetown University, Box 571412, Washington, DC 20057-1412, USA. ecelbarc@georgetown.edu
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Ecelbarger
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007674:Kidney Diseases / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D046228:Microarray Analysis; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger