Large cell calcifying Sertoli cell tumor of the testis: comparative immunohistochemical study with Leydig cell tumor.
Large cell calcifying Sertoli cell tumor is a rare type of testicular tumor. Reported herein is a Japanese patient with this tumor not associated with Carney's complex. An 11-year-old boy was admitted to hospital because of left testicular enlargement, and radical orchiectomy was performed. Macroscopically, the tumor was well circumscribed and had a maximum diameter of approximately 2 cm. The cut surface showed a yellow-white solid mass. Histologically, the tumor was composed of large neoplastic cells with abundant eosinophilic cytoplasm with a tubular, trabecular, and solid arrangement and loose myxoid stroma with irregularly shaped calcification. Immunohistochemically, the tumor cells were positive for vimentin, S-100 protein, calretinin, inhibin-alpha, melan-A, and CD10, and type IV collagen and laminin were observed in the extracellular matrix around the tumor cells. The distributions of melan-A, CD10, and mitochondria were characteristically patchy; in contrast, they were diffusely distributed in the cytoplasm in a control case of Leydig cell tumor. The differences in immunostaining patterns for melan-A, CD10, and mitochondria as well as positivity for S-100 protein-beta might be useful diagnostic hallmarks of large cell calcifying Sertoli cell tumor for discrimination from Leydig cell tumor.
Sato
Katsuaki
K
Department of Pathophysiological and Experimental Pathology, Kanazawa Medical University, Ishikawa, Japan. sato-k@kanazawa-med.ac.jp
Ueda
Yoshimichi
Y
Sakurai
Aya
A
Ishikawa
Yoshimaro
Y
Okamoto
Shin-Ya
SY
Ikawa
Hiromichi
H
Katsuda
Shogo
S
D000951:Antigens, Neoplasm; D064032:Calbindin 2; D002114:Calcinosis / Q000473:pathology*; D002648:Child; D024062:Collagen Type V / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007265:Inhibins / Q000032:analysis; D058965:MART-1 Antigen; D008297:Male; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000032:analysis; D015260:Neprilysin / Q000032:analysis; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D012707:Sertoli Cell Tumor / Q000378:metabolism / Q000473:pathology*; D013736:Testicular Neoplasms / Q000378:metabolism / Q000473:pathology*; D013737:Testis / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D014746:Vimentin / Q000032:analysis
D000951:Antigens, Neoplasm; C576764:CALB2 protein, human; D064032:Calbindin 2; D024062:Collagen Type V; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins; D064030:S100 Calcium Binding Protein G; D009418:S100 Proteins; D014746:Vimentin; C094377:inhibin-alpha subunit; D007265:Inhibins; D015260:Neprilysin