title: [Effects of macrophage migration inhibitory factor on collagen I and III mRNA expression in vascular smooth muscle cells in vitro].
abstract: OBJECTIVE
  To investigate the association between macrophage migration inhibitory factor (MIF) and collagen synthesis in vascular smooth muscle cells (VSMCs).
  
  
  METHODS
  VSMCs isolated from SD rat aorta were cultured in different culture plates, and the fourth passage of cells were divided into two groups, one of which was simulated by MIF and the other served as control group. Collagen I and III mRNA expression in the VSMCs was detected by reverse transcriptase (RT)-PCR and PCR.
  
  
  RESULTS
  Collagen I and III mRNA expression in MIF-treated VSMCs increased significantly as compared with that in the control cells (P<0.05).
  
  
  CONCLUSIONS
  MIF increases collagen I and III mRNA expression of VSMCs in vitro, indicating its involvement in collagen synthesis in VSMCs.
authors.affiliation: Institute of Cardiovascular Diseases, Guangdong People's Hospital, Guangzhou 510080, China. linzhongxiangma@sina.com
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Lin
authors.forename: Xi-yong
authors.initials: XY
authors.lastname: Yu
authors.forename: Qiu-xiong
authors.initials: QX
authors.lastname: Lin
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger