title: Reversibility of airflow obstruction by hypoglossus nerve stimulation in anesthetized rabbits.
abstract: BACKGROUND
  Anesthesia-induced uncoupling of upper airway dilating and inspiratory pump muscles activation may cause inspiratory flow limitation, thereby mimicking obstructive sleep apnea/hypopnea.
  
  
  OBJECTIVE
  Determine whether inspiratory flow limitation occurs in spontaneously breathing anesthetized rabbits and whether this can be reversed by direct hypoglossal nerve stimulation and by the application of continuous positive airway pressure.
  
  
  METHODS
  Ten New Zealand White rabbits were anesthetized, instrumented, and studied supine while breathing spontaneously at ambient pressure or during the application of positive or negative airway pressure. Under each of these conditions, the effect of unilateral or bilateral hypoglossal nerve stimulation was investigated.
  
  
  METHODS
  Inspiratory flow and tidal volume were measured together with esophageal pressure and the electromyographic activity of diaphragm, alae nasi, and genioglossus muscles.
  
  
  RESULTS
  Anesthesia caused a marked increase in inspiratory resistance, snoring, and in eight rabbits, inspiratory flow limitation. Hypoglossus nerve stimulation was as effective as continuous positive airway pressure in reversing inspiratory flow limitation and snoring. Its effectiveness increased progressively as airway opening pressure was lowered, reached a maximum at -5 cm H2O, but declined markedly at lower pressures. With negative airway opening pressure, airway collapse eventually occurred during inspiration that could be prevented by hypoglossus nerve stimulation. The recruitment characteristics of hypoglossus nerve fibers was steep, and significant upper airway dilating effects already obtained with stimulus intensities 36 to 60% of maximum.
  
  
  CONCLUSIONS
  This study supports hypoglossus nerve stimulation as a treatment option for obstructive sleep apnea.
authors.affiliation: Laboratoire du sommeil, Service de pneumologie, Centre Hospitalier de l'Université de Montréal, Hôtel-Dieu, Montréal, Quebec, H2W 1T8 Canada. bellemaf@colba.net
authors.forename: François
authors.initials: F
authors.lastname: Bellemare
authors.forename: Matteo
authors.initials: M
authors.lastname: Pecchiari
authors.forename: Monica
authors.initials: M
authors.lastname: Bandini
authors.forename: Mohamad
authors.initials: M
authors.lastname: Sawan
authors.forename: Edgardo
authors.initials: E
authors.lastname: D'Angelo
mesh_terms: D000402:Airway Obstruction / Q000209:etiology / Q000628:therapy*; D000758:Anesthesia / Q000009:adverse effects*; D000818:Animals; D045422:Continuous Positive Airway Pressure; D004558:Electric Stimulation / Q000379:methods*; D020437:Hypoglossal Nerve Diseases / Q000209:etiology / Q000628:therapy*; D001239:Inhalation / Q000502:physiology; D011817:Rabbits