title: Studies on soluble ectodomain proteins of relaxin (LGR7) and insulin 3 (LGR8) receptors.
abstract: The ectodomains of both the relaxin (LGR7) and the INSL3 (LGR8) receptors can be expressed on the cell surface using only a single transmembrane domain. These membrane-anchored proteins retain the ability to bind relaxin and can be cleaved from the cell surface. The subsequent LGR7 protein, 7BP, binds relaxin and can act as a functional relaxin antagonist. By contrast, the equivalent LGR8 protein 8BP does not bind relaxin or antagonize LGR8 activity. The 7BP protein has been successfully immobilized onto chemically derivatized surfaces for the capture of relaxin peptides and subsequent identification via SELDI-MS analysis.
authors.affiliation: Howard Florey Institute, University of Melbourne, Victoria 3010, Australia.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.forename: Sharon
authors.initials: S
authors.lastname: Layfield
authors.forename: Tania
authors.initials: T
authors.lastname: Ferraro
authors.forename: Jin
authors.initials: J
authors.lastname: Kumagai
authors.forename: Satoko
authors.initials: S
authors.lastname: Sudo
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Tang
authors.forename: Eleni
authors.initials: E
authors.lastname: Giannakis
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Tregear
authors.forename: John D
authors.initials: JD
authors.lastname: Wade
authors.forename: Ross A D
authors.initials: RA
authors.lastname: Bathgate
mesh_terms: D002460:Cell Line; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism*; D018000:Receptors, Peptide; D012065:Relaxin / Q000378:metabolism*; D012995:Solubility
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D008565:Membrane Proteins; D011506:Proteins; C417543:RXFP1 protein, human; C472867:RXFP2 protein, human; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; D012065:Relaxin