Study of tissue-specific CpG methylation of DNA in extended genomic loci.
Modern approaches for studies on genome functioning include investigation of its epigenetic regulation. Methylation of cytosines in CpG dinucleotides is an inherited epigenetic modification that is responsible for both functional activity of certain genomic loci and total chromosomal stability. This review describes the main approaches for studies on DNA methylation. Under consideration are site-specific approaches based on bisulfite sequencing and methyl-sensitive PCR, whole-genome approaches aimed at searching for new methylation hot spots, and also mapping of unmethylated CpG sites in extended genomic loci.
Azhikina
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TL
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia. tanya@humgen.siobc.ras.ru
Sverdlov
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ED
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D023281:Genomics; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013447:Sulfites / Q000737:chemistry
D013447:Sulfites; C029641:DNA modification methylase HpaII; C081573:DNA modification methylase SssI; D015257:DNA-Cytosine Methylases; C042345:hydrogen sulfite