title: Evaluation of oxidative stress measurements in obstructive sleep apnea syndrome.
abstract: Assessment of reactive oxygen species (ROS) is highly important in neurodegenerative disorders and neuroleptic treatment. However, conflicting results have been reported, which may arise from methodological difficulties. Obstructive sleep apnea (OSA) syndrome with episodic hypoxia-reoxygenation is proposed as a human model for the investigation of ROS measurements. Despite a broad analytical approach comprising lipid peroxidation and amino acid oxidation products, oxidative DNA damage, and activity of the antioxidant defense, only plasma malondialdehyde (MDA) and urinary o,o'-dityrosine seemed to be appropriate, robust biomarkers of oxidative stress, which are also simple enough for routine clinical use. MDA concentrations correlated with a duration of nocturnal desaturation below 85% (r = 0.77, p<0.0005), and o,o'-dityrosine levels decreased after therapy (p<0.05) as a function of baseline concentrations (r = -0.61, p<0.05). Gender effects in ROS generation also have to be considered. At present, we recommend the application of several oxidative stress measurements at different time points, preferably involving plasma MDA and urinary o,o'-dityrosine.
authors.affiliation: Department of Psychiatry and Psychotherapy, University of Goettingen, Goettingen, Germany. wjordan@gwdg.de
authors.forename: W
authors.initials: W
authors.lastname: Jordan
authors.forename: S
authors.initials: S
authors.lastname: Cohrs
authors.forename: D
authors.initials: D
authors.lastname: Degner
authors.forename: A
authors.initials: A
authors.lastname: Meier
authors.forename: A
authors.initials: A
authors.lastname: Rodenbeck
authors.forename: G
authors.initials: G
authors.lastname: Mayer
authors.forename: J
authors.initials: J
authors.lastname: Pilz
authors.forename: E
authors.initials: E
authors.lastname: Rüther
authors.forename: J
authors.initials: J
authors.lastname: Kornhuber
authors.forename: S
authors.initials: S
authors.lastname: Bleich
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000652:urine; D045422:Continuous Positive Airway Pressure; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D019803:Glutathione Disulfide / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008550:Melatonin / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020181:Sleep Apnea, Obstructive / Q000378:metabolism* / Q000628:therapy; D013482:Superoxide Dismutase / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives / Q000652:urine
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D014443:Tyrosine; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; C007543:dityrosine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D003849:Deoxyguanosine; D008550:Melatonin; D019803:Glutathione Disulfide