title: [Expression and secretion of human tumor necrosis factor gene transfected on human embryo myoblasts].
abstract: OBJECTIVE
  To observe human tumor necrosis factor-alpha (hTNF-alpha) expression and secreting level of human embryo myoblasts transfected by hTNF-alpha gene.
  
  
  METHODS
  Human embryo myoblasts were transfected with shuttle plasmid pSV23SHTNF containing hTNF-alpha gene by cationic liposomes DOSPER. The control group was only given equivalent liposomes except plasmid. After culturing for 24, 48, 72 and 96 hours, hTNF-alpha expression level of human embryo myoblasts was observed with immunocytochemistry staining, and hTNF-alpha secreting of human embryo myoblasts was analyzed by ELISA.
  
  
  RESULTS
  After transfected by hTNF-alpha gene for 24, 48, 72 and 96 hours, the human embryo myoblasts displayed significant secretion of hTNF-alpha in the cultural supernatant (P < 0.05), and overexpression in cytoplasma and cell membrane.
  
  
  CONCLUSIONS
  Transfection of hTNF-alpha gene to human myoblasts made myoblasts secrete high concentration of hTNF-alpha, implying it is feasible that transfecting muscle cells surrounding tongue carcinoma lesion with hTNF-alpha gene can prevent tongue carcinoma from intruding into deeper muscle tissue.
authors.affiliation: Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Zhen-Nan
authors.initials: ZN
authors.lastname: Gao
authors.forename: Jia-Rang
authors.initials: JR
authors.lastname: Cao
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Fu
mesh_terms: D000818:Animals; D015316:Genetic Therapy; D006801:Humans; D032446:Myoblasts; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D010957:Plasmids; D014062:Tongue Neoplasms / Q000628:therapy*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha