title: Nitric oxide-dependent activation of pig oocytes: role of calcium.
abstract: Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor. These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.
authors.affiliation: Research Institute of Animal Production, Prátelství 815, Prague 10, Uhríneves, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Petr
authors.forename: Radko
authors.initials: R
authors.lastname: Rajmon
authors.forename: Vilma
authors.initials: V
authors.lastname: Lánská
authors.forename: Markéta
authors.initials: M
authors.lastname: Sedmíková
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jílek
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D047028:Macrocyclic Compounds; D009569:Nitric Oxide / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010080:Oxazoles / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012430:Ruthenium Red / Q000494:pharmacology; D013552:Swine; D014700:Verapamil / Q000494:pharmacology
chemical_list: D047028:Macrocyclic Compounds; D010080:Oxazoles; C108644:xestospongin A; D012430:Ruthenium Red; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D009569:Nitric Oxide; D011343:Procaine; D004533:Egtazic Acid; D006493:Heparin; D014700:Verapamil; D002118:Calcium