A physiological product-release model for baculovirus infected insect cells.
Existing models describe the product release from baculovirus infected insect cells as an unspecific protein leakage occurring in parallel with protein production. The model presented here shows that the observed product release of normally non-secreted proteins can be described through cell death alone. This model avoids the implicit non-physiological assumption of previous models that cells permeable to recombinant protein as well as trypan blue continue to produce protein.
Haas
Richard
R
Laboratory for Biological Engineering, Department of Chemical Engineering, the University of Queensland, 4072 Brisbane, Australia.
Nielsen
Lars K
LK
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D007313:Insecta / Q000166:cytology*; D008962:Models, Theoretical; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology / Q000235:genetics
D011994:Recombinant Proteins