title: SLC26A6 and SLC26A7 anion exchangers have a distinct distribution in human kidney.
abstract: BACKGROUND
  The anion transporters SLC26A6 (PAT1) and SLC26A7, transporting at least chloride, oxalate, sulfate and bicarbonate, show a distinct expression and function in different mammalian species. They are expressed in kidney, but their exact localization in human kidney has not been studied. We therefore examined SLC26A6 and A7 expression in human kidneys.
  
  
  METHODS
  The localization of SLC26A6 and A7 in different segments of human nephrons was studied by RT-PCR and immunohistochemistry by comparing to the tubular markers PNRA, CD10, Tamm-Horsfall antigen, high molecular weight cytokeratin, CK7, AQP2 and H(+)V-ATPase.
  
  
  RESULTS
  In human kidney, SLC26A6 is expressed in distal segments of proximal tubules, parts of the thin and thick ascending limbs of Henle's loops, macula densa, distal convoluted tubules and a subpopulation of intercalated cells of collecting ducts. SLC26A7 is expressed in extraglomerular mesangial cells and a subpopulation of intercalated cells of collecting ducts.
  
  
  CONCLUSIONS
  Our results show that in human kidney SLC26A6 and A7 have a distinct, partially overlapping expression in distal segments of nephrons. The distribution partly differs from that found previously in rodent kidneys.
authors.affiliation: Department of Medical Genetics, University of Helsinki, Helsinki, Finland. minna.kujala@helsinki.fi
authors.forename: Minna
authors.initials: M
authors.lastname: Kujala
authors.forename: Jukka
authors.initials: J
authors.lastname: Tienari
authors.forename: Hannes
authors.initials: H
authors.lastname: Lohi
authors.forename: Outi
authors.initials: O
authors.lastname: Elomaa
authors.forename: Hannu
authors.initials: H
authors.lastname: Sariola
authors.forename: Eero
authors.initials: E
authors.lastname: Lehtonen
authors.forename: Juha
authors.initials: J
authors.lastname: Kere
mesh_terms: D000328:Adult; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004622:Embryo, Mammalian / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D021782:Multicystic Dysplastic Kidney / Q000378:metabolism; D009399:Nephrons / Q000378:metabolism; D016891:Polycystic Kidney, Autosomal Dominant / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000076988:Sulfate Transporters; D014018:Tissue Distribution
chemical_list: D017920:Antiporters; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C439529:SLC26A6 protein, human; C455626:SLC26A7 protein, human; D000076988:Sulfate Transporters