title: In-vitro antiproliferative effects on human tumour cell lines of extracts and jacaranone from Senecio leucanthemifolius Poiret.
abstract: We have studied the cytotoxic activity of extracts and jacaranone from Senecio leucanthemifolius Poiret. Extracts from S. leucanthemifolius were able to inhibit the in-vitro proliferation of a series of human tumour cell lines. The dichloromethane extract demonstrated effective cytotoxic activity with an IC50 of 20.1 microg mL(-1) on the large cell carcinoma cell line COR-L23. The ethyl acetate extract showed an IC50 value of 5.0 microg mL(-1) and the butanol extract an IC50 value of 6.4 microg mL(-1) on the same cell line. A major active constituent of the dichloromethane extract was shown to be jacaranone, which was demonstrated to have a very strong activity against all of the tumour cell lines with IC50 values between 2.86 and 3.85 microg mL(-1), although it did not account for all the activity observed. Constituents of S. leucanthemifolius extracts were identified by GC/MS analysis and NMR.
authors.affiliation: Department of Pharmaceutical Sciences, University of Calabria, I-87036 Arcavata di Rende, CS, Italy. mr.loizzo@unical.it
authors.forename: M R
authors.initials: MR
authors.lastname: Loizzo
authors.forename: R
authors.initials: R
authors.lastname: Tundis
authors.forename: G A
authors.initials: GA
authors.lastname: Statti
authors.forename: F
authors.initials: F
authors.lastname: Menichini
authors.forename: P J
authors.initials: PJ
authors.lastname: Houghton
mesh_terms: D016227:Benzoquinones; D001943:Breast Neoplasms / Q000473:pathology; D018938:Caco-2 Cells; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D011809:Quinones / Q000494:pharmacology*; D012674:Senecio / Q000737:chemistry*
chemical_list: D016227:Benzoquinones; D010936:Plant Extracts; D011809:Quinones; C013077:jacaranone