New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides.
A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the [M--H](-) ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moiety.
Sturiale
Luisa
L
CNR-Istituto di Chimica e Tecnologia dei Polimeri, Viale Regina Margherita 6, 95123 Catania, Italy.
Garozzo
Domenico
D
Silipo
Alba
A
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Molinaro
Antonio
A
D001419:Bacteria / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003640:Dealkylation; D007202:Indicators and Reagents; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007202:Indicators and Reagents; D008050:Lipid A; D008070:Lipopolysaccharides