title: Ultrastructural, immunohistochemical and biochemical analysis of glycosaminoglycans and proteoglycans in the mouse pubic symphysis during pregnancy.
abstract: During pregnancy, an interpubic ligament is formed in the mouse pubic symphysis. In late stages, this ligament undergoes "relaxation" to allow proper delivery, which is expected on the 19th day. Proteoglycans and hyaluronic acid play an important role in the remodeling of the extracellular matrix in these tissues. Glycosaminoglycans and proteoglycans were studied by electron microscopic, immunohistochemical and biochemical methods in samples of mouse pubic symphysis from the 12th to 18th day of pregnancy. At the ultrastructural level, using cuprolinic blue and enzymatic digestion by chondroitin lyases, two types of proteoglycan filaments were observed in the fibrocartilage on the 12th day, as well as in D 15, D 17 and D 18 pubic ligaments. The only sulfated glycosaminoglycan in these filaments was chondroitin sulfate, as shown by chondroitin lyase treatment. Their electrophoretic mobility, before and after enzymatic degradation, corroborated this inference. The ratio of chondroitin sulfate/dry weight of symphysis showed two phases of increase: between D12 and D 15, and between D 17 and D 18. We suggest that the first corresponds mainly to an increase in decorin when the ligament is formed, and the second to versican, during "relaxation". Versican and hyaluronic acid, working as water holding molecules would be responsible for the hydration of the ligament at the end of pregnancy, allowing an increase in resiliency. The presence of hyaluronic acid was confirmed by labeling with HA-probe in the perichondrium, fibrocartilage and ligament. The role of collagen fibers as physical restrictors of the complete expansion of glycosaminoglycans and hyaluronic acid in tissue is discussed.
authors.affiliation: Department of Cell Biology, Institute of Biology, UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Pinheiro
authors.forename: O A
authors.initials: OA
authors.lastname: Mora
authors.forename: E G
authors.initials: EG
authors.lastname: Caldini
authors.forename: C N
authors.initials: CN
authors.lastname: Battlehner
authors.forename: P P
authors.initials: PP
authors.lastname: Joazeiro
authors.forename: O M S
authors.initials: OM
authors.lastname: Toledo
mesh_terms: D000818:Animals; D002808:Chondroitin Lyases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure*; D006820:Hyaluronic Acid / Q000032:analysis; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011509:Proteoglycans / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure*; D011631:Pubic Symphysis / Q000378:metabolism* / Q000648:ultrastructure*; D013455:Sulfur / Q000737:chemistry
chemical_list: D006025:Glycosaminoglycans; D007211:Indoles; D009942:Organometallic Compounds; D011509:Proteoglycans; C015445:copper phthalocyanine; D013455:Sulfur; D006820:Hyaluronic Acid; D002808:Chondroitin Lyases