Polymorphism of HIV-1 gag (p17) gene from female sex workers in Calcutta, India.
HIV-1 subtype C is the major subtype in India as evidenced from the analysis of specific regions within envelope and gag gene. The matrix protein (p17) of HIV-1 which is involved in several functions like the viral RNA transport, nuclear localization, assembly of pre-integration complex into host nucleus has been used to study the strain diversity among female sexworkers in Calcutta. The gene encoding for the HIV-1 matrix protein, p17 was amplified by nested polymerase chain reaction (PCR) from blood samples of HIV-1 seropositive female sex workers (FSW) in Calcutta, India. Genes of twenty-two samples were sequenced and the phylogenetic analysis with different global strains showed that the majority (seventeen) was clustered with Indian type C. A few samples were found to be close to other C subtypes isolated from South Africa, China and Myanmar. The comparison of Calcutta samples with the samples from other regions of India along with other non-asian subtype C sequences clearly revealed a different cluster of Indian sequences. The two samples, cal 242 and cal 709 was found to be the most divergent type and showed close relatedness with African C subtypes.
Sengupta
S
S
National Institute of Cholera and Enteric Diseases, ICMR, Calcutta, India.
Khetawat
D
D
Jana
S
S
Sarkar
K
K
Bhattacharya
S K
SK
Chakrabarti
S
S
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015683:Gene Products, gag / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D015488:HIV Antigens / Q000235:genetics*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007194:India; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011477:Sex Work; D014764:Viral Proteins / Q000235:genetics*; D054301:gag Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015683:Gene Products, gag; D015488:HIV Antigens; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1