Prostate cancer and the genomic revolution: Advances using microarray analyses.
The emerging technology of microarray analysis allows the establishment of molecular portraits of prostate cancer and the discovery of novel genes involved in the carcinogenesis process. Many novel genes have already been identified using this technique, and functional analyses of these genes are currently being tested. The combination of microarray analysis with other recently developed high-throughput techniques, such as proteomics, tissue arrays, and gene promoter-methylation, especially using tissue microdissection methods, will provide us with more comprehensive insights into how prostate cancer develops and responds to gene-targeted therapies. Animal models of prostate cancer are being characterized by high throughput techniques to better define the similarities and differences between those models and the human disease, and to determine whether particular models may be useful for specific targeted therapies in pre-clinical studies. Although profiling of mRNA expression provides important information of gene expression, the development of proteomic technologies will allow for an even more precise global insight into cellular signaling and structural alterations during prostate carcinogenesis. Not only will the "omic" revolution change basic science, but it will lead to a new era of molecular medicine.
Calvo
Alfonso
A
Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, NIH, Building 41, Bethesda, MD 20892, USA.
Gonzalez-Moreno
Oscar
O
Yoon
Cheol-Yong
CY
Huh
Jung-Im
JI
Desai
Kartiki
K
Nguyen
Quang Tri
QT
Green
Jeffrey E
JE
D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism
D014408:Biomarkers, Tumor