Cholesterol-bile salt vesicles as potential delivery vehicles for drug and vaccine delivery.
The aim of this study was to further investigate the interactions between cholesterol (CH) and mixed bile salts (BS) (sodium cholate and sodium deoxycholate) and their suitability for drug and vaccine delivery. Insulin was used as a model protein to assess the ability of CH:BS vesicles to entrap a therapeutically relevant macromolecule. The association of protein (FITC-insulin) with the CH:BS structure was confirmed with fluorescence microscopy, and the overall morphology of the vesicles was examined with atomic force microscopy (AFM). Results demonstrate that the nature of the vesicles formed between CH and BS is dependent not only on the concentration of BS but also on the increasing CH concentration leading to CH crystal formation.
Martin
C
C
Vaccine Delivery Group, Centre for Drug Delivery Research, University of London, School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
Thongborisute
J
J
Takeuchi
H
H
Yamamoto
H
H
Kawashima
Y
Y
Alpar
H O
HO
D001647:Bile Acids and Salts / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D016503:Drug Delivery Systems; D004563:Electrochemistry; D016650:Fluorescein-5-isothiocyanate; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry; D008823:Micelles; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D014677:Pharmaceutical Vehicles; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry
D001647:Bile Acids and Salts; D007328:Insulin; D008823:Micelles; D014677:Pharmaceutical Vehicles; D014612:Vaccines; D002784:Cholesterol; D016650:Fluorescein-5-isothiocyanate