Chiral separation of anticholinergic drug enantiomers in nonaqueous capillary electrophoresis.
Nonaqueous capillary electrophoretic (NACE) method for the separation of nine structurally similar chiral anticholinergic drugs was developed. The eight drug enantiomers were separated on baseline within 18 min using 20mM phosphoric acid and 10 mM NaOH, containing 10 mM heptakis(2,3-dimethyl-6-sulfato)-4beta-cyclodextrin (HDMS-beta-CD) in methanol. The results were compared with those obtained in the high performance liquid chromatography system.
Du
Guohua
G
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
Zhang
Shuzhen
S
Xie
Jianwei
J
Zhong
Bohua
B
Liu
Keliang
K
D018680:Cholinergic Antagonists / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D018680:Cholinergic Antagonists; D047392:beta-Cyclodextrins; C400661:heptakis(2,3-dimethyl-6-sulfato)cyclodextrin