Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos.
OBJECTIVE
To investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome (BWS) in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS.


METHODS
Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.


RESULTS
Transcripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos.


CONCLUSIONS
Transcripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.
Shen
Wen-jie
WJ
Reproductive Medical Center, Nanfang Hospital, Guangzhou, Guangdong, 510515, PR China.
Xing
Fu-qi
FQ
Kong
Ling-hong
LH
Chen
Shi-ling
SL
Li
Hong
H
D001506:Beckwith-Wiedemann Syndrome / Q000235:genetics*; D001755:Blastocyst / Q000378:metabolism*; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics; D009865:Oocytes / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050761:Cyclin-Dependent Kinase Inhibitor p57; C485621:KCNQ1OT1 long non-coding RNA, human; D009687:Nuclear Proteins; D024642:Potassium Channels, Voltage-Gated; C109277:TSSC3 protein