[Expression of phytase gene in Bombyx mori].
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
Wang
Wen-Bing
WB
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang 212018, China.
Yao
Bin
B
Xiao
Qing-Li
QL
Ji
Ping
P
Wang
Sheng-Peng
SP
He
Jia-Lu
JL
Wu
Xiang-Fu
XF
D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006863:Hydrogen-Ion Concentration
D005656:Fungal Proteins; D010832:6-Phytase