Hessian fly resistance gene H13 is mapped to a distal cluster of resistance genes in chromosome 6DS of wheat.
H13 is inherited as a major dominant resistance gene in wheat. It was previously mapped to chromosome 6DL and expresses a high level of antibiosis against Hessian fly (Hf) [Mayetiola destructor (Say)] larvae. The objective of this study was to identify tightly linked molecular markers for marker-assisted selection in wheat breeding and as a starting point toward the map-based cloning of H13. Fifty-two chromosome 6D-specific microsatellite (simple sequence repeat) markers were tested for linkage to H13 using near-isogenic lines Molly (PI 562619) and Newton-207, and a segregating population consisting of 192 F(2:3) families derived from the cross PI 372129 (Dn4) x Molly (H13). Marker Xcfd132 co-segregated with H13, and several other markers were tightly linked to H13 in the distal region of wheat chromosome 6DS. Deletion analysis assigned H13 to a small region closely proximal to the breakpoint of del6DS-6 (FL 0.99). Further evaluation and comparison of the H13-linked markers revealed that the same chromosome region may also contain H23 in KS89WGRC03, an unnamed H gene (H(WGRC4)) in KS89WGRC04, the wheat curl mite resistance gene Cmc4, and a defense response gene Ppo for polyphenol oxidase. Thus, these genes comprise a cluster of arthropod resistance genes. Marker analysis also revealed that a very small intercalary chromosomal segment carrying H13 was transferred from the H13 donor parent to the wheat line Molly.
Liu
X M
XM
Department of Entomology and Plant Science and Entomology Research Unit, USDA-ARS, Kansas State University, Manhattan, 66506, USA.
Gill
B S
BS
Chen
M-S
MS
D000818:Animals; D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004175:Diptera; D017343:Genes, Plant / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology*; D014908:Triticum / Q000235:genetics*