Differential gene expression of collagen-binding small leucine-rich proteoglycans and lysyl hydroxylases, during mineralization by MC3T3-E1 cells cultured on titanium implant material.
Titanium implants create a unique ultrastructure (composed of a collagenous zone with relatively disorganized fibril morphology) at the bone-implant interface. The objective of this study was to investigate the temporal mRNA expression patterns, using real-time polymerase chain reaction, of type I collagen (COLI) and regulators for collagen fibrillogenesis, collagen-binding small leucine-rich proteoglycans (SLRPs) and lysyl hydroxylases (LHs), during mineralization, by MC3T3-E1 cells cultured on titanium (Ti). Lysates of the cultures on Ti and on plastic wells (Pl) for 10-50 d were used for the quantification of calcium and mRNA. Although the onset of calcium accumulation in the cultures on Ti (30-40 d) was slower than that of cultures on Pl (20-30 d), the gene expression patterns during mineralization were similar in cells cultured on either material. COLI and fibromodulin were up-regulated just before the onset of mineralization and then down-regulated. Lumican and LH1 were up-regulated just before the onset of mineralization and then returned to the baseline level. Decorin and LH2 were up-regulated at the late mineralization stage. Biglycan was down-regulated once at the early mineralization stage and then returned to the original level. LH3 was maintained at a steady level throughout. This study suggests actual but distinct roles of SLRPs and LHs in the formation of a unique ultrastructure at the bone-implant interface.
Takashi
Matsuura
M
Department of Oral Rehabilitation, Fukuoka Dental College, Tamura 2-15-1, Sawara-ku, Fukuoka 814-0193, Japan. matsuurt@college.fdcnet.ac.jp
Tsubaki
Satoshi
S
Tsuzuki
Takashi
T
Duarte
Wagner R
WR
Yamauchi
Mitsuo
M
Sato
Hironobu
H
D016475:3T3 Cells; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology*; D002118:Calcium / Q000032:analysis; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000235:genetics; D024042:Collagen Type I / Q000032:analysis* / Q000235:genetics; D058575:Decorin; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics; D000071235:Fibromodulin; D005786:Gene Expression Regulation / Q000235:genetics; D007632:Keratan Sulfate / Q000032:analysis / Q000235:genetics; D007930:Leucine / Q000032:analysis* / Q000235:genetics; D000071234:Lumican; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010969:Plastics / Q000737:chemistry; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000032:analysis* / Q000235:genetics; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014025:Titanium / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D015854:Up-Regulation
D011508:Chondroitin Sulfate Proteoglycans; D024042:Collagen Type I; C546879:Dcn protein, mouse; D058575:Decorin; D015921:Dental Implants; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; C000604307:Lum protein, mouse; D000071234:Lumican; D010969:Plastics; D011509:Proteoglycans; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000071235:Fibromodulin; D007632:Keratan Sulfate; D014025:Titanium; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D007930:Leucine; D002118:Calcium