The human LGR7 low-density lipoprotein class A module requires calcium for structure.
The relaxin and INSL3 receptors, LGR7 and LGR8, are the only human G-protein-coupled receptors to contain a low-density lipoprotein class-A (LDL-A) module. LDL-A modules are well characterized in a variety of diverse biological functions that involve ligand binding to elicit a response. Common features of the LDL-A modules characterized to date are the conservation of three disulfide bonds and the structural arrangement around a bound calcium ion. In this study we recombinantly produce the human LGR7 LDL-A module for NMR studies and demonstrate that calicum is required for the module to form a stable and correctly folded structure.
Hopkins
Emma J
EJ
The Howard Florey Institute of Experimental Physiology, University of Melbourne, Parkville, Australia.
Bathgate
Ross A
RA
Gooley
Paul R
PR
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology*; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000145:classification; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D011487:Protein Conformation / Q000187:drug effects; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018000:Receptors, Peptide; D016415:Sequence Alignment; D012995:Solubility
D004220:Disulfides; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; C417543:RXFP1 protein, human; C472867:RXFP2 protein, human; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; D002118:Calcium