[Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis].
OBJECTIVE
Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4.


METHODS
Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry.


RESULTS
During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little. These data confirmed that HSCs had the core effect on liver fibrogenesis and apoptosis may be a major factor regulating HSCs numbers during the injury-fibrosis-recovery sequence. At each time point, the number of activated HSCs in pathological group is more than intervention group, while apoptotic HSCs are less, which suggested dietary supplement with antioxidative nutrients had effect on HSC apoptosis but more studies are necessary to make the mechanism clearer.


CONCLUSIONS
Dietary supplement with proper vitamin E and selenium can effectively lighten the hepatic fibrosis and promote the recovery of hepatocyte and the degradation of the existing collagens, ie, it is beneficial to the recovery of hepatic fibrosis.
Shen
Xiu-hua
XH
Department of Nutrition, Shanghai Second Medical University, Shanghai 200025, China.
Cheng
Wu-feng
WF
Li
Xuan-hai
XH
Xie
Liang-min
LM
Sun
Jianqin
J
Li
Feng
F
Ma
Lin
L
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002251:Carbon Tetrachloride; D049109:Cell Proliferation / Q000187:drug effects; D019587:Dietary Supplements; D022781:Hepatocytes / Q000187:drug effects*; D008106:Liver Cirrhosis, Experimental / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D002251:Carbon Tetrachloride; D012643:Selenium