title: Isolation and cloning of the raccoon (Procyon lotor) papillomavirus type 1 by using degenerate papillomavirus-specific primers.
abstract: Partial sequences of a novel papillomavirus were amplified from a cutaneous lesion biopsy of a raccoon (Procyon lotor), by using PCR with degenerate papillomavirus-specific primers. The Procyon lotor papillomavirus type 1 (PlPV-1) DNA was amplified with long template PCR in two overlapping fragments, together encompassing the entire genome, and the complete PlPV-1 genomic sequence was determined. The PlPV-1 genome consists of 8170 bp, and contains the typical papillomaviral open reading frames, encoding five early proteins and two late capsid proteins. Besides the classical non-coding region (NCR1) between the end of L1 and the start of E6, PlPV-1 contains an additional non-coding region (NCR2) of 1065 bp between the early and late protein region, which has previously also been described for the canine oral papillomavirus (COPV) and the Felis domesticus papillomavirus (FdPV-1). Phylogenetic analysis places PlPV-1 together with COPV and FdPV-1 in a monophyletic branch which encompasses the Lambda papillomavirus genus.
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Annabel
authors.initials: A
authors.lastname: Rector
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Koenraad
authors.initials: K
authors.lastname: Van Doorslaer
authors.affiliation: Toronto Zoo, Ontario M1B 5K7, Canada.|Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ontario N1G 2W1, Canada.
authors.forename: Mads
authors.initials: M
authors.lastname: Bertelsen
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ontario N1G 2W1, Canada.
authors.forename: Ian K
authors.initials: IK
authors.lastname: Barker
authors.affiliation: Toronto Zoo, Ontario M1B 5K7, Canada.|Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ontario N1G 2W1, Canada.
authors.forename: Rolf-Arne
authors.initials: RA
authors.lastname: Olberg
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lemey
authors.affiliation: The Jackson Laboratory, Bar Harbor, ME 04609-1500, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Sundberg
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011821:Raccoons / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins