Expression of feline interferon-alpha subtypes in Esherichia coli, and their antiviral activity and animal species specificity.
Two kinds of FeIFN-alpha consisting of 166 amino acids (aa) and 171 aa were expressed in Escherichia coli, and the purified proteins were tested for antiviral activity on homologous and heterologous animal cells. Crude FeIFN induced in feline cells revealed antiviral activity on both homologous and heterologous animal cells. In contrast, both types of recombinant FeIFN-alpha revealed antiviral activity only on the feline cells. All of the FeIFN-alpha subtypes showed high activity to vesicular stomatitis virus, and the three species of feline viruses belonging to different families.
Taira
Osamu
O
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, USA.
Suzuki
Makoto
M
Takeuchi
Yuko
Y
Aramaki
Yoshitaka
Y
Sakurai
Itsuki
I
Watanabe
Takao
T
Motokawa
Kenji
K
Arai
Setsuo
S
Sato
Hisaaki
H
Maehara
Nobutoshi
N
D000818:Animals; D017927:Calicivirus, Feline / Q000276:immunology; D002415:Cats / Q000276:immunology* / Q000821:virology; D004926:Escherichia coli; D016766:Feline Infectious Peritonitis / Q000276:immunology; D015870:Gene Expression; D006564:Herpesviridae / Q000276:immunology; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000502:physiology*; D030781:Organisms, Genetically Modified; D013045:Species Specificity; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014780:Viruses / Q000276:immunology*
D016898:Interferon-alpha