Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene.
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
Park
Hyo-Soon
HS
Department of Microbiology, Konkuk University, Chungju, Chungchongbuk-Do, Korea.
Lee
Jung-Hee
JH
Jeong
Eun-Ju
EJ
Kim
Jung-Eun
JE
Hong
Sung-Jin
SJ
Park
Tae-Kyu
TK
Kim
Tae-Yung
TY
Jang
Won-Jong
WJ
Park
Kyung-Hee
KH
Kim
Bum-Joon
BJ
Kook
Yoon-Hoh
YH
Lee
Seung-Hyun
SH
D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000453:epidemiology* / Q000276:immunology; D017422:Sequence Analysis, DNA; D014437:Typhus, Endemic Flea-Borne / Q000453:epidemiology* / Q000276:immunology
D018834:Chaperonin 60; D004275:DNA, Ribosomal