Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB.
EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment that encodes the C-terminus of EhGPCR-1. The cloning and sequence of the full-length cDNA revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocytosis and seven transmembranal domains. These results suggest that EhGPCR-1 could be a GPCR involved in phagocytosis. EhGPCR-1 could be a member of the Rhodopsin family, characterized by a short N-terminus without cysteine residues.
Picazarri
Karina
K
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, A.P. 14-740, Mexico, D.F. 07000, Mexico.
Luna-Arias
Juan Pedro
JP
Carrillo
Eduardo
E
Orozco
Esther
E
Rodriguez
Mario A
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000502:physiology; D010802:Phylogeny; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
D018076:DNA, Complementary; D043562:Receptors, G-Protein-Coupled; D020691:rab GTP-Binding Proteins