title: [Inhibition effects of par-4 antisense oligodeoxynucleotide on apoptosis of PC12 cell induced by glutamate is mediated by ERK1/2].
abstract: OBJECTIVE
  To investigate the inhibition effects of par-4 antisense oligodeoxynucleotide on apoptosis of PC12 cell induced by glutamate and its signal transduction mechanism.
  
  
  METHODS
  (1) Cationic lipid-mediated par-4 antisense oligodeoxynucleotide (Par-4-AS-ODN) was transfected into PC12 cells before they were treated with glutamate. Mismatch oligodeoxynucleotide (MS-ODN) were also transfected into cells as controls. (2) Morphological observation and the detection of anti-apoptosis effects of par-4-AS-ODN on PC12 cells were done with the Laser Scanning confocal Microscope by double staining the cells with acridine orange/ethidium bromide (AO/EB), addition to with flow cytometry. (3) Western blot was used to detect the protein levels of par-4 and phosphorylated ERK(1/2) at threonine-202 and Tyrosine-204.
  
  
  RESULTS
  (1) Par-4-AS-ODN significantly suppressed up-regulation of the par-4 protein levels induced by glutamate in PC12 cells. (2) Par-4-AS-ODN could resist the decrease of phosphorylated ERK(1/2) (Thr202/Tyr204) induced by glutamate in PC12 cells. (3) Par-4 AS-ODN could inhibit apoptosis of PC12 cells induced by glutamate. But its inhibition effect could be eliminated by PD98059, a selective MEK(1) inhibitor which could inhibit phosphorylation of ERK(1/2).
  
  
  CONCLUSIONS
  Par-4 AS-ODN may inhibit apoptosis of PC12 cells induced by glutamate, and its inhibition effects may be medicated by the activation of ERK(1/2).
authors.affiliation: Department of Pediatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Ji-qing
authors.initials: JQ
authors.lastname: Chen
authors.forename: Sheng-hua
authors.initials: SH
authors.lastname: Wu
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Chi
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Fei
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Song-ming
authors.initials: SM
authors.lastname: Huang
authors.forename: Xi-rong
authors.initials: XR
authors.lastname: Guo
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000494:pharmacology*; D015536:Down-Regulation; D018698:Glutamic Acid / Q000494:pharmacology*; D019950:Mitogen-Activated Protein Kinase 1 / Q000096:biosynthesis / Q000235:genetics; D048052:Mitogen-Activated Protein Kinase 3 / Q000096:biosynthesis* / Q000235:genetics; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; D020319:Oligodeoxyribonucleotides, Antisense; C101296:prostate apoptosis response-4 protein; D018698:Glutamic Acid; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3