[Spectrum of gene expression of a multi-drug resistant leukemia cell line with high tumorigenicity in nude mice].
OBJECTIVE
To investigate the mechanism of multi-drug resistance of K562-n/VCR cell line with both bcr-abl and mdr-1 expressions by clustering analysis of differential gene expression profiles.


METHODS
By DNA microarray technique, genes differentially expressed by K562-n/VCR and K562-n cell lines were identified and analyzed.


RESULTS
DNA microarray analysis of K562-n/VCR and K562-n cells was repeated three times and revealed 58 genes significantly differentially expressed among 12,800 genes arrayed. All but one was up-regulated in K562-n/VCR cells. The only gene down-regulated was a-myb. The up-regulated genes were MDR-associated genes, oncogenes, cytoskeleton, protein kinases and phosphatases, apoptotic and antiapoptotic factors, metabolism, transcriptional regulators associated with stress response, cell cycle checkpoint control, and genes for signal transduction proteins.


CONCLUSIONS
These results indicate that, besides MDR-associated genes, other known and unknown genes may also be involved in the mechanism of multi-drug resistance.
Chen
Li
L
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Xu
Xiao-ping
XP
Wang
Jian-min
JM
Li
Yao
Y
Xu
Hong
H
Lü
Shu-qing
SQ
Fei
Xin-hong
XH
Huang
Zheng-xia
ZX
D000818:Animals; D018432:Drug Resistance, Multiple / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D051379:Mice; D008819:Mice, Nude; D020411:Oligonucleotide Array Sequence Analysis; D014750:Vincristine / Q000494:pharmacology
D014750:Vincristine