title: Comparison of four media types during 3-day human IVF embryo culture.
abstract: The aim of this study was to compare the effectiveness of human tubal fluid (HTF), G1.2, Sage Cleavage and Life Global media for IVF outcome during 3-day culture of human embryos. A three-phase auto-controlled study was conducted in which IVF outcome was compared between (1) HTF and G1.2, (2) HTF and Cleavage, and (3) Cleavage and Life Global. In phase 1, no differences in embryo quality were observed between HTF and G1.2. However, embryos derived from intracytoplasmic sperm injection (ICSI) displayed significantly improved quality when grown in HTF versus G1.2. No differences in pregnancy and implantation rates were observed in cases where embryos transferred were grown exclusively in HTF or G1.2 media. In phase 2, embryo quality was significantly improved for embryos cultured in Cleavage versus HTF media (P < 0.001). However, pregnancy, implantation and spontaneous abortion rates were similar between the two media. In phase 3, there were no differences in embryo quality, pregnancy, implantation, and spontaneous abortion rates between Cleavage and Life Global media. Overall, the data indicate that Life Global and Cleavage media yield similar results in a 3-day IVF culture programme. Cleavage medium is superior to HTF, as evidenced by significantly improved embryo quality (P < 0.001). Meanwhile, HTF medium is superior to G1.2 for ICSI cases.
authors.affiliation: Andrology Laboratory, University of Utah School of Medicine, Salt Lake City, UT 84108, USA.
authors.forename: Vincent W
authors.initials: VW
authors.lastname: Aoki
authors.forename: Aaron L
authors.initials: AL
authors.lastname: Wilcox
authors.forename: C Matthew
authors.initials: CM
authors.lastname: Peterson
authors.forename: Kirtly
authors.initials: K
authors.lastname: Parker-Jones
authors.forename: Harry H
authors.initials: HH
authors.lastname: Hatasaka
authors.forename: Mark
authors.initials: M
authors.lastname: Gibson
authors.forename: Ivan
authors.initials: I
authors.lastname: Huang
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Carrell
mesh_terms: D002970:Cleavage Stage, Ovum; D003470:Culture Media / Q000737:chemistry*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies
chemical_list: D003470:Culture Media