Comparison of early passage, senescent and hTERT immortalized endothelial cells.
The need for standardized experimental conditions to gain relevant and reproducible results has increased the demand for well characterized continuously growing cell lines that exhibit the characteristics of their normal counterparts. Immortalization of normal human cells by ectopic expression of the catalytic subunit of human telomerase (hTERT) has shown to result in highly differentiated cell lines. However, the influence of the increased telomerase activity on the protein expression profile was not investigated so far. Therefore, we have immortalized human umbilical vein endothelial cells (HUVECs) by hTERT overexpression and compared them to their normal early passage and senescent counterparts. This study, including a proteomic approach, shows that ectopic hTERT expression leads to a stable growing cell line. Although these cells are highly differentiated, the protein expression profile of the cell line is different to that of normal early passage and senescent cells.
Chang
Martina Wei-Fen
MW
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
Grillari
Johannes
J
Mayrhofer
Corina
C
Fortschegger
Klaus
K
Allmaier
Günter
G
Marzban
Gorji
G
Katinger
Hermann
H
Voglauer
Regina
R
D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000166:cytology* / Q000473:pathology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D016133:Polymerase Chain Reaction; D040901:Proteomics; D019098:Telomerase / Q000235:genetics; D014162:Transfection
D004268:DNA-Binding Proteins; D019098:Telomerase