title: The growth profiles of three types of canine distemper virus on Vero cells expressing canine signaling lymphocyte activation molecule.
abstract: To know growth profiles of canine distemper virus (CDV) on Vero cells stably expressing canine signaling lymphocyte activation molecule (Vero-DogSLAMtag; Vero-DST cells), the propagation of three strains of CDV was tested in Vero-DST cells in comparison with parental Vero cells. Strain MD77 could grow well in both cell lines, but demonstrated no syncytium formation or indistinguishable rounding cytopathic effects (CPE) in Vero cells. Strains Onderstepoort and KDK-1 also grew well in Vero-DST cells with apparent syncytium CPE, while they grew less or no efficiently, respectively, in Vero cells. All three CDV strains demonstrated the peak titers, in Vero-DST cells before reaching to an extensive CPE and drastic decrease of titers at/after full CPE. Immunohistochemistry revealed that viral antigens of all CDV strains were found exclusively in the syncytia in Vero-DST cells, while in Vero cells, viral antigen was identified in their single cells for strain MD77 but none for other strains. Thus, every strain of CDV could grow well in Vero-DST cells and behaved differently against Vero cells. These results would be of practical value for workers of CDV because 1) In Vero-DST cells, by observation of distinct syncytium CPE, the highest titer or the best growth of virus could be identified; 2) In Vero cells, various CDV strains could be readily classified after propagation in Vero-DST cells.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Lan
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kai
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.forename: Susumu
authors.initials: S
authors.lastname: Tateyama
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000956:Antigens, Viral; D002522:Chlorocebus aethiops; D004217:Distemper Virus, Canine / Q000145:classification / Q000254:growth & development*; D004285:Dogs; D015870:Gene Expression; D006023:Glycoproteins / Q000502:physiology*; D007136:Immunoglobulins / Q000502:physiology*; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000502:physiology*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary
chemical_list: D015703:Antigens, CD; D000956:Antigens, Viral; D006023:Glycoproteins; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1