title: Directed evolution and Phenomics screening of new biocatalysts.
abstract: Biodiversity screening and directed evolution are two fruitful complementary approaches for the discovery and design of novel biocatalysts. A new technology for directed evolution, L-Shuffling, has been designed and patented by Proteus. L-Shuffling technology offers several competitive advantages over other technologies including (i) directed evolution of large genes: L-Shuffling" means "Large-Shuffling"; (ii) high fidelity recombination and (iii) Control over location and frequency of recombination. The thousands of new recombinants generated by L-Shuffling can be further screened for their biochemical characteristics using Phenomics. Phenomics is a proprietary functional HTS technology designed and patented by Proteus for the screening of natural biodiversity as well as biodiversity generated by combinatorial biology. Phenomics is a function to gene structure approach which provides an alternative to genomics and proteomics. The traditional limits of expression libraries are thereby circumvented especially those related to cytotoxic products in usual or specific surrogate hosts. The quality of the answer given by the screening is directed dependent on the quality of the question asked. Thanks to a new substrates synthesis technology named CLIPS-O, the company can design highly specific molecules simulating the chemical structure and energetic state of the industrial substrates. The whole process of novel biocatalysts discovery has been automated using commercially available high throughput robotics.
authors.affiliation: Protéus SA, Parc Scientifique George Besse, 70 allée Graham Bell, 3000 Nîmes, France.
authors.forename: F
authors.initials: F
authors.lastname: Lefevre
authors.forename: G
authors.initials: G
authors.lastname: Ravot
authors.forename: H K
authors.initials: HK
authors.lastname: Nguyen
authors.forename: C
authors.initials: C
authors.lastname: Ayrinhac
authors.forename: S
authors.initials: S
authors.lastname: Pallas
authors.forename: J M
authors.initials: JM
authors.lastname: Masson
authors.forename: D
authors.initials: D
authors.lastname: Dupret
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002175:Candida / Q000201:enzymology / Q000235:genetics; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D030541:Databases, Genetic; D019020:Directed Molecular Evolution; D007221:Industry; D019712:Thermococcus / Q000201:enzymology / Q000235:genetics