title: Replaceable cross-linked polyacrylamide for high performance separation of proteins.
abstract: An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matrix generated high resolutions for a wide range (approximately 4 kD to approximately 300 kD) of proteins. When compared to the most frequently used sieving matrixes, the rCPA permitted the highest resolutions with comparable or increased separation speed for protein separations.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA.
authors.forename: Joann J
authors.initials: JJ
authors.lastname: Lu
authors.forename: Shaorong
authors.initials: S
authors.lastname: Liu
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Pu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000592:standards; D005782:Gels / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013997:Time Factors; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D005782:Gels; D011506:Proteins; D000099166:Polyacrylamides