title: Polymorphisms of B7 (CD80 and CD86) genes do not affect disease susceptibility to sarcoidosis.
abstract: BACKGROUND
  B7 proteins (CD80, CD86) are costimulatory molecules expressed on antigen-presenting cells and are essential factors for T cell activation.
  
  
  OBJECTIVE
  The aim of this study is to investigate the relationship of B7 gene polymorphisms either to disease susceptibility or to cell profile of bronchoalveolar lavage (BAL) fluid in Japanese sarcoidosis patients.
  
  
  METHODS
  Gene polymorphisms located in CD80 promoter, CD80 exon 3 and exon 8 of CD86 were examined in 146 Japanese sarcoidosis patients and 157 healthy controls using single-strand conformation polymorphism and direct sequencing. The distribution of genotypes was compared between the two groups. BAL fluid cell profiles were compared for the various genotypes of the different polymorphisms for the 62 patients who underwent BAL.
  
  
  RESULTS
  There were no significant differences in the distribution of genotypes or allele frequencies for all polymorphisms between sarcoidosis and controls. There were no significant differences in BAL fluid cell profiles among the different genotype groups of the various polymorphisms studied.
  
  
  CONCLUSIONS
  There was no relationship between the B7 gene polymorphisms studied and disease susceptibility or BAL fluid cell profiles in Japanese sarcoidosis patients.
authors.affiliation: Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Shogoin Kawaracho, Kyoto, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Handa
authors.forename: Sonoko
authors.initials: S
authors.lastname: Nagai
authors.forename: Isao
authors.initials: I
authors.lastname: Ito
authors.forename: Rollin
authors.initials: R
authors.lastname: Tabuena
authors.forename: Michio
authors.initials: M
authors.lastname: Shigematsu
authors.forename: Kunio
authors.initials: K
authors.lastname: Hamada
authors.forename: Masanori
authors.initials: M
authors.lastname: Kitaichi
authors.forename: Takateru
authors.initials: T
authors.lastname: Izumi
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Junya
authors.initials: J
authors.lastname: Toguchida
authors.forename: Michiaki
authors.initials: M
authors.lastname: Mishima
mesh_terms: D015703:Antigens, CD / Q000235:genetics*; D018122:B7-1 Antigen / Q000235:genetics*; D051940:B7-2 Antigen; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic / Q000235:genetics*; D012507:Sarcoidosis / Q000235:genetics* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins