Enzyme-linked immunosorbent assay for detection of canine chromogranin A by use of immunological cross-reactivity of rabbit anti-bovine chromogranin A antibody.
Bovine and canine chromogranin A were extracted and purified from each specie's adrenal glands. Isolated bovine 70 kDa protein showed 100% identity to bovine CgA reported previously, whereas isolated canine 68 kDa protein showed 83.3% identity to bovine CgA by the NH(2)-terminal amino acid sequence analysis. Rabbit antibody to purified bovine protein (CgA) was found to immunologically cross-reacted with purified canine protein (CgA). In sandwich ELISA with anti-bovine CgA, concentration-dependent curves were obtained ranging from 0.3 to 20 mug/ml for canine CgA. From these findings, sandwich ELISA with anti-bovine CgA is found to be useful to determine the concentration of canine CgA.
Akiyoshi
Hideo
H
Laboratory of Veterinary Surgery, Department of Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai-shi, Osaka 599-8531, Japan.
Aoki
Mica
M
Kumagai
Daijiro
D
Saleh
Nahed
N
Noda
Katsura
K
Shimada
Terumasa
T
Sugii
Shunji
S
Ohashi
Fumihito
F
D000818:Animals; D000906:Antibodies; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011817:Rabbits; D013045:Species Specificity
D000906:Antibodies; D053379:Chromogranin A; D002864:Chromogranins