Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.
Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.
Nienhaus
Karin
K
Department of Biophysics and General Zoology, University of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany.
Nienhaus
G Ulrich
GU
Wiedenmann
Jörg
J
Nar
Herbert
H
D000818:Animals; D037421:Anthozoa; D001224:Aspartic Acid / Q000737:chemistry; D018360:Crystallography, X-Ray; D004583:Electrons; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D007532:Isoleucine / Q000737:chemistry; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry; D014965:X-Rays; D000097573:Red Fluorescent Protein
D001224:Aspartic Acid; D006639:Histidine; D007532:Isoleucine; D008164:Luminescent Proteins; D010455:Peptides; D010649:Phenylalanine; D011506:Proteins; D014867:Water; D000097573:Red Fluorescent Protein