Adding value to fusion proteins through covalent labelling.
Recombinant proteins are often expressed with an additional polypeptide the role of which is to aid purification, detection or functional studies. Recently, the role of these tags has been extended to mediate the labelling of the protein of interest with chemically diverse compounds. These approaches are of importance for protein science as they allow proteins to be equipped with properties that cannot be genetically encoded and also enable the use of a single fusion protein for several different applications. Several new approaches have been developed for the covalent labelling of fusion proteins both in living cells and in vitro.
Gronemeyer
Thomas
T
Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering, CH-1015 Lausanne, Switzerland.
Godin
Guillaume
G
Johnsson
Kai
K
D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods
D011993:Recombinant Fusion Proteins