[Telomerase RNA antisense oligonucleotides inhibit growth of human choriocarcinoma xenograft in nude mice].
OBJECTIVE
To study the inhibitory effect of antisense oligonucleotides against telomerase RNA on the growth of human choriocarcinoma transplant in nude mice.


METHODS
Choriocarcinoma xenografts were established by transplanting JAR cells subcutaneously to female nude mice, and were treated with high and low doses of antisense oligonucleotides. Control groups were treated with NS, random sequence and actinomycin D (Act-D). Tumor growth was monitored once every other day. Telomerase relative activity was assayed by TRAP-ELISA. Western blotting was used to detect expression of hTERT.


RESULTS
Low and high doses antisense oligonucleotides, and Act-D inhibited tumor growth by 76.6%, 93.8% and 85.4% respectively, which were significantly different when compared with random sequence and NS groups. Expression of telomerase relative activity and hTERT were decreased as well. But the differences among the first three groups had no significance.


CONCLUSIONS
Telomerase RNA antisense oligonucleotide inhibits growth of human choriocarcinoma xenografts in nude mice. It may be a novel approach to the treatment of choriocarcinoma.
Chen
Li-li
LL
Women's Hospital, Medical College of Zhejiang University, Hangzhou 310006, China. chenglili-@163.com
Zheng
Wei
W
Chen
Xue-jun
XJ
Wan
Liang
L
Shi
Yi-fu
YF
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000201:enzymology / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014594:Uterine Neoplasms / Q000201:enzymology / Q000473:pathology*
D000903:Antibiotics, Antineoplastic; D004268:DNA-Binding Proteins; D016376:Oligonucleotides, Antisense; D003609:Dactinomycin; D019098:Telomerase