title: [Study on multi-lineage potential of bone marrow mesenchymal stem cells derived from green fluorescent protein transgenic mice].
abstract: OBJECTIVE
  To study the multi-lineage potential of bone marrow mesenchymal stem cells (MSCs) derived from transgenic mice with green fluorescent protein (GFP) gene in vitro.
  
  
  METHODS
  A 6-week-old GFP transgenic mouse was executed by dislocation of cervical vertebra, and the marrow in tibia and thighbone was washed out with asepsis. The limited cell strains of MSCs derived from GFP transgenic mice (GFP-MSCs) were obtained with density gradient centrifugation. The passage 3 GFP-MSCs were induced to differentiate into osteoblast, adippcyte, neuron with solution of calcium induction medium, adipogenic medium and neural induction medium respectively. After being calcium-induced, the activity of alkaline phosphatase on GFP-MSCs was determined by micro-plate reader, and alizarin red staining was performed to test the formation of calcium concentration. The adipo-induced MSCs were detected with oil red O staining. Immunocytochemical staining was performed to detect the expression of NSE on neuron-induced MSCs.
  
  
  RESULTS
  The ALP activity of GFP-MSCs heightened gradually along with being calcium-induced, and alizarin red staining showed positive. Oil red O staining of adipo-induced cells and NSE immunocytochemical staining of neuron-induced cells demonstrated positive.
  
  
  CONCLUSIONS
  The limited cell strain of GFP-MSCs possesses multi-lineage potential, which can be used as an efficient tracking facility for studying the mechanism of multi-lineage potential on the MSCs.
authors.affiliation: Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xi-Zhe
authors.initials: XZ
authors.lastname: Chen
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Lin
authors.forename: Zheng-Bin
authors.initials: ZB
authors.lastname: Yan
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons; D010006:Osteoblasts
chemical_list: D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase