[Analysis of chromosome karyotype of oral carcinoma-associated fibroblasts].
OBJECTIVE
The purpose of this study was to investigate whether the fundamental genetic character of oral carcinoma-associated fibroblasts changes through contrasting and analyzing the oral carcinoma-associated fibroblasts and the normal fibroblasts of oral mucosa.


METHODS
The two kinds of cells were treated with colchicine and microsometic fluid, and then were expanded with cold acetic acid and formalized with methyl alcohol. The cells were observed under the oil microscope after Giemsa staining. The chromosome karyotype of the two kinds of cells was analyzed by Visus 2. 1.


RESULTS
There were not obvious differences in the way of chromosome karyotype between the oral carcinoma-associated fibroblasts and the normal fibroblasts of oral mucosa.


CONCLUSIONS
The basic genetic characteristics of the normal cells are conserved in the oral carcinoma-associated fibroblasts, which means the cells have no malignant changes.
Zheng
Xiao-Hui
XH
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Liu
Ying
Y
Zhou
Hong-Mei
HM
Chen
Qian-Ming
QM
Li
Bing-Qi
BQ
D002875:Chromosomes; D005347:Fibroblasts; D006801:Humans; D059785:Karyotype; D007621:Karyotyping; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000235:genetics*