Molecular cloning of the canine c-Met/HGF receptor and its expression in normal and regenerated liver.
The c-Met proto-oncogene is the receptor for hepatocyte growth factor (HGF), which is a member of the tyrosine kinase family. Activation of the HGF/c-Met signal pathway leads to cell proliferation, motility, regeneration, and morphogenesis. In this study, the complete nucleotide sequence of complementary DNA (cDNA) of canine c-Met was cloned, and its distribution was determined in tissues. The canine c-Met cDNA clone had an open reading frame of 4419 bp that encoded a putative polypeptide of 1383 amino acids. The c-Met mRNA was expressed in a variety of canine tissues including peripheral blood mononuclear cells (PBMC), bone marrow, liver, kidney, lung, stomach, uterus, testis, thymus, lymph node, small intestine, colon, adrenal gland, thyroid gland, heart, muscle, skin, pancreas, ovary, prostate, spleen, fat, cerebrum, and cerebellum. In addition, the c-Met mRNA expression in normal and regenerated liver was examined. The levels of the mRNA increased 2-fold in regenerated liver compared to that found in normal liver, indicating that c-Met is involved in various functions including remodeling of canine hepatocytes.
Neo
Sakurako
S
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara-shi, Kanagawa 229-8501, Japan.
Kansaku
Norio
N
Furuichi
Mitsuru
M
Watanabe
Masashi
M
Hisamatsu
Sin
S
Ohno
Koichi
K
Hisasue
Masaharu
M
Tsuchiya
Ryo
R
Yamada
Takatsugu
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004285:Dogs; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008969:Molecular Sequence Data; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis* / Q000235:genetics
D019859:Proto-Oncogene Proteins c-met