title: [Expression of MLRQ subunit gene of NADH oxidoreductase and its clinical significance in malignant tumors of digestive system].
abstract: OBJECTIVE
  To study the differential expression and possible role of MLRQ subunit gene of nicotinamide adenine dinucleotide reduced (NADH) oxidoreductase in malignant tumors of digestive system.
  
  
  METHODS
  Specimens of cancerous tissues and matched adjacent normal tissues resected during operation or biopsy: 38 pairs of specimens of esophageal carcinoma, 7 pairs of specimens of cardiac carcinoma, 14 pairs of specimens of gastric carcinoma, 11 pairs of specimens of colon carcinoma, and 7 pairs of specimens of liver carcinoma underwent PCR test and Northern hybridization to detect the differential expression of MLRQ subunit gene of NADH oxidoreductase.
  
  
  RESULTS
  (1) Overexpression of MLRQ subunit gene was found in 31 of the 38 pairs of specimens of esophageal carcinoma (81.6%), 4 of the 7 pairs of specimens of cardiac carcinoma (57.1%), 12 of the 14 pairs of specimens of gastric carcinoma (85.7%), 4 of the 7 pairs of specimens of colon carcinoma (65.6%), and 7 of the 11 pairs of specimens of liver cancer (57.1%). No significant difference among different cancers was observed by X(2) test (all P > 0.05). (2) The up-regulation of MLRQ subunit was not correlated with clinic stage, infiltration degree, lymphatic metastasis, and differentiation of tumor (all P > 0.05).
  
  
  CONCLUSIONS
  MLRQ subunit gene is up-regulated in the malignant tumors of digestive system.
authors.affiliation: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Ze-jian
authors.initials: ZJ
authors.lastname: Li
authors.forename: Li-yong
authors.initials: LY
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Guo-bin
authors.initials: GB
authors.lastname: Sun
authors.forename: Xiu-qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D004067:Digestive System Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation
chemical_list: D009247:NADH, NADPH Oxidoreductases