title: Systematic analysis and overall toxicity evaluation of dioxins and hexachlorobenzene in human milk.
abstract: A systematic method for analyzing dioxins (PCDDs, PCDFs and dioxin-like PCBs), hexachlorobenzene (HCB), heptachlor epoxide and beta-hexachlorocyclohexane (HCH) in human milk was developed to determine the residual amount of HCB in human milk and to evaluate the overall toxicity of both dioxins and HCB in human milk. The fractionation behavior of HCB on chromatography with silica gel, alumina, and activated carbon/silica gel, and the concentrated sulfuric acid decomposition method, which is widely used as a dioxin cleanup method, were studied in order to make the preprocessing operation for HCB measurement compatible with that for conventional dioxin measurement. HCB was found to be eluted in the 2% dichloromethane (DCM)/hexane 60 ml fraction from an alumina column. Heptachlor epoxide and a part of beta-HCH were eluted in the 10% DCM/hexane 50 ml fraction from a silica gel column, while the remaining beta-HCH was eluted in the 25% DCM/hexane 60 ml fraction from an activated carbon/silica gel column. Moreover, HCB showed significant correlation with dioxin congeners having high toxicity equivalence factors (TEFs). The results suggest that the exposure route to HCB and its accumulation behavior in the human body are similar to those of the dioxins.
authors.affiliation: Department of Analytical Chemistry, Hoshi University, 2-4-41 Ebara, Tokyo 142-8501, Japan. k-saito@hoshi.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ogawa
authors.forename: Mikiko
authors.initials: M
authors.lastname: Takekuma
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohmura
authors.forename: Migaku
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Rie
authors.initials: R
authors.lastname: Ito
authors.forename: Koichi
authors.initials: K
authors.lastname: Inoue
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Matsuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D002623:Chemistry Techniques, Analytical; D004147:Dioxins / Q000032:analysis* / Q000633:toxicity*; D004785:Environmental Pollutants / Q000032:analysis* / Q000633:toxicity*; D005260:Female; D006581:Hexachlorobenzene / Q000032:analysis* / Q000633:toxicity*; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D012016:Reference Values; D018570:Risk Assessment; D018675:Toxicity Tests
chemical_list: D004147:Dioxins; D004785:Environmental Pollutants; D006581:Hexachlorobenzene