Determination of antioxidants in cosmetics by micellar electrokinetic capillary chromatography with electrochemical detection.
A new and efficient method for the determination of antioxidants [Propyl gallate (PG), tert-butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT)] in cosmetics has been established by using micellar electrokinetic capillary chromatography with electrochemical detection (MECC-ED). Under the optimum conditions of the method, all analytes were successfully separated within 13 min at the separation voltage of 18 kV in a 20 mmol/L borate running buffer (pH 7.4) containing 25 mmol/L sodium dodecyl sulfate. The excellent linearity was obtained in the concentration range from 5.0 x 10(-4) to 2.0 x 10(-6) mol/L and the detection limits (S/N = 3) of PG, TBHQ, BHA, and BHT range from 3 x 10(-7) to 3 x 10(-6) mol/L.
Guan
Yueqing
Y
Department of Chemistry, East China Normal University, Shanghai 200062, China.
Chu
Qingcui
Q
Fu
Liang
L
Ye
Jiannong
J
D000975:Antioxidants / Q000032:analysis*; D002083:Butylated Hydroxyanisole / Q000302:isolation & purification; D002084:Butylated Hydroxytoluene / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000302:isolation & purification; D011435:Propyl Gallate / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate
D000975:Antioxidants; D003358:Cosmetics; D006873:Hydroquinones; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D012967:Sodium Dodecyl Sulfate; D011435:Propyl Gallate; C018855:2-tert-butylhydroquinone