[Identification and sequence analysis of a novel HLA-B*5614 allele].
OBJECTIVE
To investigate the molecular genetics basis for a novel HLA allele, HLA-B*5614, in Chinese population.


METHODS
DNA was extracted from whole blood by salting-out method. The HLA-B exons 2-4 of the proband was amplified and the amplified product was cloned using TOPO cloning sequencing kit to split the two alleles apart. Both strands of exons 2,3 and 4 of chosen colonies were sequencing. The PCR-SSP was performed to confirm the mutations detected by sequencing.


RESULTS
The sequencing results showed the HLA-B alleles of the proband as B*1502 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY601726, AY610727, AY610728). After BLAST analysis, the novel allele differs from B*5608 by a single nucleotide at position 277G-->C in exon 2. This results in an amino acid change from Gly to Arg at codon 93.


CONCLUSIONS
This allele is a novel allele and has been officially named B*5614 by the WHO Nomenclature Committee.
Zhu
Fa-ming
FM
Blood Center of Zhejiang Province, Hangzhou, Zhejiang, 310006, PR China. zfm00@hotmail.com
Lu
Qin-feng
QF
Zhang
Wei
W
Zhang
Hai-qin
HQ
Fu
Qi-hua
QH
Yan
Li-xing
LX
D000483:Alleles; D005091:Exons / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
D015235:HLA-B Antigens