Imaging phosphoinositide dynamics using GFP-tagged protein domains.
Phosphoinositides are important regulators of cellular homoeostasis and numerous signal-transduction pathways. One of their major features is their ability to recruit signalling proteins to membranes by direct interaction with phosphoinositide-binding modules. The distribution and dynamics of membrane phosphoinositides are therefore major determinants in the spatiotemporal control of cell signalling and membrane trafficking. However, standard biochemical approaches cannot reveal the dynamics of phosphoinositides at the single-cell level. A major technical advance has been the development of genetically encoded fluorescent phosphoinositide probes on the basis of the phosphoinositide-binding domains found in signalling proteins, such as the PH (pleckstrin homology) domain. This review describes the diverse fluorescent phosphoinositide probes available for imaging specific phosphoinositide species and how their use has improved the understanding of phosphoinositide signalling at the single-cell level.
Halet
Guillaume
G
Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK. g.halet@ucl.ac.uk
D000818:Animals; D001665:Binding Sites; D003952:Diagnostic Imaging; D004705:Endocytosis; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D051379:Mice; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010587:Phagocytosis; D010716:Phosphatidylinositols / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
D005456:Fluorescent Dyes; D010716:Phosphatidylinositols; D049452:Green Fluorescent Proteins; D010744:Phosphoric Monoester Hydrolases