[Development of proteome two-dimensional electrophoresis technology].
Two-dimensional electrophoresis (2-DE) is the core technology of proteome study. This paper mainly introduces the 2-DE principle, sample preparation and usual methods to improve its resolving power. Furthermore, isoelectric focusing, SDS-PAGE electrophoresis and 2-DE staining methods are also reviewed.
Du
Peng
P
Department of Military Hygiene, Sencond Military Medical University, Shanghai 200433, China.
Feng
Wei-hua
WH
Guo
Jun-sheng
JS
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007525:Isoelectric Focusing; D020543:Proteome / Q000032:analysis*; D013194:Staining and Labeling
D020543:Proteome