A high performance liquid chromatography method for the simultaneous determination of arctiin, chlorogenic acid and glycyrrhizin in a Chinese proprietary medicine.
A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of arctiin, chlorogenic acid and glycyrrhizin in the tablets of a Chinese proprietary medicine named, "Yin Qiao Jie Du Pian". The analysis was performed by a reverse phase gradient elution, using an aqueous mobile phase (containing 0.4% acetic acid and 4.5% tetrahydrofuran) modified by acetonitrile and detection made simultaneously at three wavelengths. The method was validated for specificity, accuracy, precision and limits of detection and quantification. Tablets of seven commercial brands were analyzed and found to contain different amounts of the three bioactive markers. This raised the question of the quality and the efficacy of the products. The method developed can be used for the quality control of "Yin Qiao Jie Du" tablets.
Yuan
Xudong
X
Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL 33328, USA.
Koh
Hwee-Ling
HL
Chui
Wai-Keung
WK
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000032:analysis; D015415:Biomarkers; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D004345:Drug Industry; D005663:Furans / Q000032:analysis* / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D013997:Time Factors; D014466:Ultraviolet Rays
D000097:Acetonitriles; D015415:Biomarkers; D005663:Furans; D005960:Glucosides; D002726:Chlorogenic Acid; C018674:tetrahydrofuran; D019695:Glycyrrhizic Acid; D019342:Acetic Acid; C077992:arctiin; C032159:acetonitrile