Improved protein identification through the use of unstained gels.
In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.
Loiselle
David R
DR
Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Thelin
William R
WR
Parker
Carol E
CE
Dicheva
Nedyalka N
NN
Kesner
Barry A
BA
Mocanu
Viorel
V
Wang
Frank
F
Milgram
Sharon L
SL
Warren
Maria R Esteban
MR
Borchers
Christoph H
CH
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D005782:Gels; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000592:standards; D012371:Robotics; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005782:Gels; D011506:Proteins