title: [Study on the direct MAIPA technique in the differential diagnosis of immune and non-immune thrombocytopenia].
abstract: OBJECTIVE
  To evaluate the clinical usefulness of direct monoclonal antibody immobilization of platelet antigen (MAIPA) technique in the differential diagnosis of immune and non-immune thrombocytopenia.
  
  
  METHODS
  Platelet-bound autoantibodies in thrombocytopenic patients (immune and non-immune) were measured by direct MAIPA. Monoclonal antibodies against GP II b/III a, GPIb and GP I a/II a were used.
  
  
  RESULTS
  The positive rates of platelet-bound GP-specific autoantibodies between immune (76.4%) and non-immune thrombocytopenia (3.6%) were significantly different (P < 0.05). The direct MAIPA had a sensitivity of 76.4%, a specificity of 96.4%, and a positive predictive value of 97.1% for the diagnosis of immune thrombocytopenia. There was a significant inverse correlation between platelet-bound GP II b/III a specific autoantibody levels and platelet counts (r = -0.338, P < 0.05).
  
  
  CONCLUSIONS
  The direct MAIPA technique can be used to differentiate immune from non-immune thrombocytopenias.
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qin
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.forename: Jian-zhi
authors.initials: JZ
authors.lastname: Sun
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zhu
authors.forename: Li-zhen
authors.initials: LZ
authors.lastname: Li
authors.forename: Mao-hong
authors.initials: MH
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011696:Purpura, Thrombocytopenic / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D010980:Platelet Membrane Glycoproteins