Continuous measurements of a binding reaction using a capacitive biosensor.
A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the muM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-occupied binding sites and (iii) the rate of disappearance of available binding sites is scaled as the number of available binding sites and analyte concentration in solution. Deconvolution of the curves using the mathematical model indicates clearly that it is possible to retrieve concentration profiles (isocratic, linearly or exponentially increasing gradients) of the analyte in the continuous sample flow from the normalized integral binding (NIB) curves. The data presented constitutes the theoretical background and the first step towards the development of an analytical system allowing on-line detection of the concentration profile of the analyte from NIB-curves. Since the system can be used for extended time periods between regeneration steps, a low frequency of regeneration steps can be expected.
Hedström
Martin
M
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Galaev
Igor Yu
IY
Mattiasson
Bo
B
D000906:Antibodies / Q000378:metabolism*; D001666:Binding Sites, Antibody; D015374:Biosensing Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold; D006801:Humans; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D012709:Serum Albumin; D006046:Gold