Adult islets cultured in collagen gel transdifferentiate into duct-like cells.
OBJECTIVE
To establish a model of islet-ductal cell transdifferentiation to identify the transdifferentiated cells.


METHODS
Collagen was extracted from rat tail at first. Purified rat islets were divided into three groups, embedded in collagen gel and incubated respectively in DMEM/F12 alone (control group), DMEM/F12 plus epidermal growth factor (EGF), DMEM/F12 plus EGF and cholera toxin (CT). Transdifferentiation was proved by microscopy, RT-PCR, immunohistochemistry and RIA.


RESULTS
Islets embedded in collagen gel plus EGF and CT were cystically transformed and could express new gene cytokeratin 19 while still maintaining the expression of insulin and Pdx-1 genes. Immunohistochemistry demonstrated that the protein of cytokeratin 19 was only expressed in the third group. The insulin content secreted by islets in the third group decreased significantly during the transdifferentiation.


CONCLUSIONS
CT is a crucial factor for the islet-ductal cell transdifferentiation.
Lu
Jin
J
Department of Biochemistry, Xiangya School of Medicine, Central South University, Xiangya Road, Changsha 410078 Hunan Province, China.
Gu
Ya-Peng
YP
Xu
Xia
X
Liu
Mei-Lian
ML
Xie
Ping
P
Song
Hui-Ping
HP
D000367:Age Factors; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D005782:Gels; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D010183:Pancreatic Ducts / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005782:Gels; D003094:Collagen