title: Gastrin-releasing peptide receptors in normal and neoplastic human uterus: involvement of multiple tissue compartments.
abstract: BACKGROUND
  Bombesin-like neuropeptides including gastrin-releasing peptide (GRP) and their corresponding receptors, mediate multiple physiological actions and have biological significance in cancer. However, information about the function of these neuropeptides and the incidence, distribution, density, and subtype of their receptors in human uterine tissues is scarce.
  
  
  OBJECTIVE
  The objective of the study was to investigate normal and neoplastic human uterine tissues for their bombesin receptor status.
  
  
  METHODS
  In vitro subtype-specific bombesin receptor autoradiography was used in this study.
  
  
  METHODS
  The following tissue samples were taken immediately after surgery: myometrium (n = 41), endometrium (n = 29), leiomyomas (n = 26), leiomyosarcomas (n = 6), endometrial adenocarcinomas (n = 28), and carcinosarcoma (n = 1).
  
  
  RESULTS
  Normal uterine tissues expressed GRP receptors (GRP-Rs) in the myometrium, in subsets of secretory endometrial glands, and in subsets of endometrial blood vessels of the late proliferative and the secretory phase. Most leiomyomas (20 of 26) expressed GRP-R but not the leiomyosarcomas. GRP-Rs were also detected in 10 of 28 adenocarcinomas, one of one carcinosarcoma, and in blood vessels surrounding the adenocarcinomas. No other bombesin receptor subtypes (neuromedin B receptors and bb3) were detected.
  
  
  CONCLUSIONS
  These findings may be of physiological and pathophysiological significance. The expression of GRP-R in glands and vessels during specific phases of the cycle suggests a timely precise physiological action of GRP in these targets; in certain uterine neoplasms, the GRP-R overexpression may contribute to tumor development because GRP is a potent growth factor. Furthermore, these findings may be diagnostically and therapeutically relevant. The expression of GRP-R in leiomyomas may allow distinguishing them from receptor-negative leiomyosarcomas; GRP-R in leiomyomas, in a subset of endometrial adenocarcinomas, carcinosarcomas, and in peritumoral vessels may be candidates for receptor targeting in vivo.
authors.affiliation: Division of Cell Biology and Experimental Cancer Research, Institute of Pathology, University of Berne, Murtenstrasse 31, P.O. Box 62, CH-3010 Berne, Switzerland.
authors.forename: Achim
authors.initials: A
authors.lastname: Fleischmann
authors.forename: Beatrice
authors.initials: B
authors.lastname: Waser
authors.forename: Jan-Olaf
authors.initials: JO
authors.lastname: Gebbers
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Reubi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D001345:Autoradiography; D002296:Carcinosarcoma / Q000378:metabolism; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism*; D007890:Leiomyosarcoma / Q000378:metabolism; D018004:Receptors, Bombesin / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
chemical_list: D018004:Receptors, Bombesin