title: Tailoring the plant metabolome without a loose stitch.
abstract: Metabolic engineering holds great promise as a technique for improving crop plants. However, introducing new metabolic steps can disturb normal metabolism and gene expression, affecting phenotype and quality in undesired ways. Recently, Charlotte Kristensen et al. reported that introducing the sorghum pathway for biosynthesis of the cyanogenic glucoside dhurrin into Arabidopsis plants resulted in high dhurrin levels and only marginal side effects on the metabolome and the transcriptome.
authors.affiliation: Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. jmemelink@biology.leidenuniv.nl
authors.forename: Johan
authors.initials: J
authors.lastname: Memelink
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods; D009570:Nitriles / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D045868:Sorghum / Q000378:metabolism*
chemical_list: D009570:Nitriles; C011220:dhurrin