title: Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach.
abstract: A number of protein markers for oral cancer are still not applicable in large populations. Proteomic technologies provide excellent tools for rapid screening of a large number of potential biomarkers in malignant cells. To gain insight into the molecular mechanisms of carcinogenesis and to identify potential biomarkers for oral squamous cell carcinomas (OSCCs), we performed proteomic profiling between human normal oral keratinocytes (HNOKs) and OSCC-derived cell lines (HSC-2 and HSC-3) using fluorescent two-dimensional difference in-gel electrophoresis. Proteins with a > or =2-fold change in expression were considered significant. The spots of interest were digested and identified by matrix-assisted laser desorption/ionization time-of-flight peptide mass finger-printing. Twenty-two proteins were identified as differentially expressed between the HNOKs and OSCC-derived cell lines. Of these, 9 spots were up-regulated and 13 were down-regulated in OSCC-derived cell lines compared to the HNOKs. These spots included the cancer-related proteins; annexin A1, heat shock protein 27, lamin A/C, interleukin 1 receptor antagonist, serine proteinase inhibitor clade B5, stathmin 1, and superoxide dismutase 2. Our results are a first step toward identifying a protein profile of HNOKs and OSCC-derived cell lines. The identified proteins in this experiment may be used in future studies of carcinogenesis or as diagnostic markers and therapeutic targets for OSCC.
authors.affiliation: Department of Clinical Molecular Biology, Chiba University, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Koike
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uzawa
authors.forename: Dai
authors.initials: D
authors.lastname: Nakashima
authors.forename: Ken
authors.initials: K
authors.lastname: Shimada
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Kato
authors.forename: Morihiro
authors.initials: M
authors.lastname: Higo
authors.forename: Yukinao
authors.initials: Y
authors.lastname: Kouzu
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Endo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasamatsu
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001331:Automation; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D010455:Peptides; D013482:Superoxide Dismutase