title: Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors.
abstract: We isolated multipotential mesenchymal stem cells (MSC) from human postnatal dermal tissues. The isolated cells were expanded and maintained for over 100 population doublings with retention of their chromosomal complement and potential for multilineage differentiation. Progeny of cell lines established from a single dermal MSC could be differentiated into adipogenic, osteogenic, and myogenic lineages, consistent with the conclusion that we established a clonal, multipotential, somatic MSC cell line. This study is the first to show potential growth of multipotential human MSC cell lines from a single cell that can be used for the engineering of tissues in vivo. Clonal growth of MSCs presents profound implications in our understanding of differentiation and development, and should provide a valuable resource for tissue repair.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: Georg
authors.initials: G
authors.lastname: Bartsch
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
authors.forename: Paolo
authors.initials: P
authors.lastname: De Coppi
authors.forename: M Minhaj
authors.initials: MM
authors.lastname: Siddiqui
authors.forename: Gunter
authors.initials: G
authors.lastname: Schuch
authors.forename: Hans G
authors.initials: HG
authors.lastname: Pohl
authors.forename: Jasmin
authors.initials: J
authors.lastname: Fuhr
authors.forename: Laura
authors.initials: L
authors.lastname: Perin
authors.forename: Shay
authors.initials: S
authors.lastname: Soker
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D002944:Circumcision, Male; D002999:Clone Cells; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D007223:Infant; D008297:Male; D024510:Muscle Development; D010012:Osteogenesis; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers