[Study on the oxidative injury of the vascular endothelial cell affected by PM2.5].
OBJECTIVE
To study the mechanism of cardiovascular disease affected by PM2.5.


METHODS
ECV304 cells were exposed to PM2.5 of different concentration (50, 200 and 400 microg/ml), after 24h, the viability of cells by MTT, SOD and GSH contents in cells and apoptosis of cells determined by flow cytometer were measured.


RESULTS
Viability of ECV304 cells declined and mortality of ECV304 cells increased gradually with increase of concentration, GSH contents in cells (mg/g prot) were 20.643 +/- 2.167, 16.774 +/- 2.911 (P < 0.05), 15.658 +/- 3.471 (P < 0.01), and SOD contents in cells (U/mg prot) were 5.878 +/- 0.401, 5.140 +/- 0.448 (P < 0.01), 4.817 +/- 0.451 (P < 0.01) when the concentration of PM2.5 was 50, 200 and 400 microg/ml.


CONCLUSIONS
PM2.5 can cause vascular endothelial cells to die by way of oxidative injury, then induce cardiovascular disease.
Dong
Chen
C
School of Public Health of Fudan University, Shanghai 200032, China.
Song
Wei-min
WM
Shi
Ye-wen
YW
D000393:Air Pollutants / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
D000393:Air Pollutants; D013482:Superoxide Dismutase; D005978:Glutathione