RET expression does not change with age in the substantia nigra pars compacta of rhesus monkeys.
Parkinson's disease is characterized by bradykinesia, rigidity and a resting tremor and the underlying basis for those symptoms is the loss of dopaminergic cells in the nigrostriatal system. Similar to PD, an age-related decrease locomotor activity and the expression of tyrosine hydroxylase immunoreactivity has been observed in rhesus monkeys, but the reason for this decrease in dopaminergic function remains to be elucidated. Trophic factors such as glial cell line derived neurotrophic factor (GDNF) and neurturin sustain the dopaminergic phenotype in midbrain neurons and act through a common receptor tyrosine kinase (RET). Examination of RET expression by immunohistochemistry was performed on sections of tissue containing the substantia nigra pars compacta of young, middle, and old aged rhesus monkeys. Stereological estimates of the number and cellular area of RET-immunoreactive cells found no change with age. Estimation of changes in RET protein using fluorescence intensity measurement was also similar across age groups. The results indicate that the mechanisms of GDNF and neurturin signaling remain intact with age, and therefore these trophic factors may be able to enhance the dopaminergic function of neurons in the nigrostriatal system, when administered to individuals of any age.
Dass
Biplob
B
Department of Neurological Sciences, Rush University Medical Center, 1735 W. Harrison Street, Chicago, IL 60657, USA.
Kladis
Theodora
T
Chu
Yaping
Y
Kordower
Jeffrey H
JH
D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D015870:Gene Expression / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008253:Macaca mulatta; D009474:Neurons / Q000378:metabolism; D051096:Proto-Oncogene Proteins c-ret / Q000378:metabolism*; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism*
D051096:Proto-Oncogene Proteins c-ret