title: Diagnosis of cerebral cryptococcoma using a computerized analysis of 1H NMR spectra in an animal model.
abstract: Viable cryptococci load in biopsy material from an animal model of cerebral cryptococcoma were correlated with 1H NMR spectra and metabolite profiles. A statistical classification strategy was applied to distinguish among high-resolution 1H NMR spectra acquired from cryptococcomas, glioblastomas, and normal brain tissue. The overall classification accuracy was 100% when a genetic-algorithm-based optimal region selection preceded the development of linear discriminant analysis-based classifiers. The method remained robust despite differences in the microbial load of the cryptococcoma group when harvested at different time points. These results indicate the feasibility of the method for diagnosis without isolation of the pathogenic microorganism and its potential for in vivo diagnosis based on computerized analysis of magnetic resonance spectra.
authors.affiliation: Institute for Magnetic Resonance Research, P.O. Box 148, New South Wales 2065, Australia.
authors.forename: Theresa E
authors.initials: TE
authors.lastname: Dzendrowskyj
authors.forename: Brion
authors.initials: B
authors.lastname: Dolenko
authors.forename: Tania C
authors.initials: TC
authors.lastname: Sorrell
authors.forename: Rajmund L
authors.initials: RL
authors.lastname: Somorjai
authors.forename: Richard
authors.initials: R
authors.lastname: Malik
authors.forename: Carolyn E
authors.initials: CE
authors.lastname: Mountford
authors.forename: Uwe
authors.initials: U
authors.lastname: Himmelreich
mesh_terms: D000465:Algorithms; D000818:Animals; D001927:Brain Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000469:parasitology*; D003455:Cryptococcus neoformans / Q000254:growth & development*; D003937:Diagnosis, Differential; D016002:Discriminant Analysis; D004195:Disease Models, Animal; D005909:Glioblastoma / Q000175:diagnosis / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008297:Male; D016919:Meningitis, Cryptococcal / Q000175:diagnosis* / Q000378:metabolism / Q000469:parasitology; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D051381:Rats; D011920:Rats, Inbred WF