Decreased stability of photosystem I in dgd1 mutant of Arabidopsis thaliana.
The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex lacking stroma side subunits was also present. PSI subunits in the dgd1 mutant were decreased to a similar level compared with that in the wild type (WT) Arabidopsis. Further experiments showed that PSI subunits in the stroma side, PsaD and PsaE, in the dgd1 mutant were more susceptible to removal by chaotropic agents than those in the WT plant, indicating that the stability of PsaD and PsaE is impaired in the dgd1 mutant. These results provide evidence that DGDG is important for the stability of the PSI complex.
Guo
Jinkui
J
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Zhang
Zhuzhu
Z
Bi
Yurong
Y
Yang
Wen
W
Xu
Yinong
Y
Zhang
Lixin
L
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D045331:Photosystem I Protein Complex / Q000032:analysis / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D020524:Thylakoids / Q000201:enzymology
D029681:Arabidopsis Proteins; D045331:Photosystem I Protein Complex; D021122:Protein Subunits; C119967:DGD1 protein, Arabidopsis; D005700:Galactosyltransferases