title: Efficient engraftment of primary adult T-cell leukemia cells in newborn NOD/SCID/beta2-microglobulin(null) mice.
abstract: Adult T-cell leukemia (ATL) develops via multiple oncogenic steps in human T-cell leukemia virus type I (HTLV-I) carriers. To better understand pathogenesis of ATL, we developed a novel xenogeneic engraftment model in which primary ATL cells are intravenously transplanted into neonatal nonobese diabetic (NOD)/severe-combined immunodeficiency (SCID)/beta2-microglobulin(null) (NOD/SCID/beta2m(null)) mice. Acute-type ATL cells engrafted in the peripheral blood and in the lymph nodes of recipients at a high efficiency. Engrafted ATL cells were dually positive for human CD4 and CD25, and displayed patterns of HTLV-I integration identical to those of donors by Southern blot analysis. These cells infiltrated into recipients' liver, and formed nodular lesions, recapitulating the clinical feature of each patient. In contrast, in smoldering-type ATL cases, multiple clones of ATL cells engrafted efficiently in NOD/SCID/beta2m(null) mice. When smoldering-type ATL cells were retransplanted into secondary NOD/SCID/beta2m(null) recipients, single HTLV-I-infected clones became predominant, suggesting that clones with dominant proliferative activity can be competitively selected in this xenogeneic system. Taken together, the NOD/SCID/beta2m(null) newborn system is useful to understand kinetics, metastasis, and disease progression of ATL in vivo.
authors.affiliation: Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medicine, Fukuoka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kawano
authors.forename: F
authors.initials: F
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Shimoda
authors.forename: M
authors.initials: M
authors.lastname: Yasukawa
authors.forename: K
authors.initials: K
authors.lastname: Nagafuji
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: E
authors.initials: E
authors.lastname: Baba
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: H
authors.initials: H
authors.lastname: Gondo
authors.forename: T
authors.initials: T
authors.lastname: Otsuka
authors.forename: K
authors.initials: K
authors.lastname: Ohshima
authors.forename: L D
authors.initials: LD
authors.lastname: Shultz
authors.forename: K
authors.initials: K
authors.lastname: Akashi
authors.forename: M
authors.initials: M
authors.lastname: Harada
mesh_terms: D000754:Anemia, Refractory, with Excess of Blasts / Q000473:pathology; D000818:Animals; D001769:Blood; D049109:Cell Proliferation; D002999:Clone Cells / Q000473:pathology; D004195:Disease Models, Animal; D006085:Graft Survival; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000209:etiology / Q000473:pathology*; D017254:Leukemic Infiltration; D008099:Liver; D008198:Lymph Nodes; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D001613:beta 2-Microglobulin / Q000235:genetics
chemical_list: D001613:beta 2-Microglobulin