Thermodynamic and dynamic factors involved in the stability of native protein structure in amorphous solids in relation to levels of hydration.
The internal, dynamical fluctuations of protein molecules exhibit many of the features typical of polymeric and bulk small molecule glass forming systems. The response of a protein's internal molecular mobility to temperature changes is similar to that of other amorphous systems, in that different types of motions freeze out at different temperatures, suggesting they exhibit the alpha-beta-modes of motion typical of polymeric glass formers. These modes of motion are attributed to the dynamic regimes that afford proteins the flexibility for function but that also develop into the large-scale collective motions that lead to unfolding. The protein dynamical transition, T(d), which has the same meaning as the T(g) value of other amorphous systems, is attributed to the temperature where protein activity is lost and the unfolding process is inhibited. This review describes how modulation of T(d) by hydration and lyoprotectants can determine the stability of protein molecules that have been processed as bulk, amorphous materials. It also examines the thermodynamic, dynamic, and molecular factors involved in stabilizing folded proteins, and the effects typical pharmaceutical processes can have on native protein structure in going from the solution state to the solid state.
Hill
John J
JJ
ICOS Corporation, 22021 20th Avenue SE, Bothell, WA 98021, USA. jjhill@icos.com
Shalaev
Evgenyi Y
EY
Zografi
George
G
D001702:Biopharmaceutics; D001704:Biopolymers / Q000737:chemistry; D003451:Cryoprotective Agents / Q000737:chemistry; D003460:Crystallization; D003890:Desiccation; D004355:Drug Stability; D004356:Drug Storage; D005612:Freeze Drying; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013696:Temperature; D013816:Thermodynamics; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*
D001704:Biopolymers; D003451:Cryoprotective Agents; D011506:Proteins; D012996:Solutions; D014867:Water