3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis.
Saturated straight- and branched-chain 3-hydroxy fatty acids (3-OH FAs) of 10-18 carbon chain lengths were determined in saliva from 27 individuals with chronic periodontitis and 18 healthy individuals by using gas chromatography-tandem mass spectrometry. Of the 14 different 3-OH FAs detected, 3-OH-C(i17:0) was the most abundant in the periodontitis samples while 3-OH-C(14:0) was the most abundant in the healthy individuals. Considering the relative percentages of 3-OH-C(12:0), 3-OH-C(14:0), 3-OH-C(i17:0), and 3-OH-C(17:0), 95.6% of all cases were correctly classified as healthy individuals or periodontitis patients by means of discriminant analysis. The sensitivity, specificity, positive predictive value and negative predictive value of 3-OH FA analysis in diagnosing peridontitis were, respectively, 0.92, 1.00, 1.00, and 0.90. The results indicate that 3-OH FA analysis of saliva samples is a useful diagnostic method in chronic periodontitis.
Ferrando
Raúl
R
Medicina Oral y Periodoncia, Clínica Odontológica Universitaria, Hospital Universitario Morales Meseguer, Avenida Marqués de los Vélez s/n, 30008 Murcia, Spain.
Szponar
Bogumila
B
Sánchez
Arturo
A
Larsson
Lennart
L
Valero-Guillén
Pedro L
PL
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002908:Chronic Disease; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006900:Hydroxylation; D008875:Middle Aged; D015394:Molecular Structure; D010518:Periodontitis / Q000175:diagnosis* / Q000378:metabolism; D011237:Predictive Value of Tests; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
D015415:Biomarkers; D005227:Fatty Acids