Inhibitory effect of glycolipids from spinach on in vitro and ex vivo angiogenesis.
Anti-cancer activity of some glycolipids from animals and plants has been demonstrated, although it was unknown whether the glycolipids had anti-angiogenic activity. The effects of the purified three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), and sulfoquinovosyl diacylglycerol (SQDG) from the green vegetable spinach (Spinacia oleracea L.) were examined on in vitro and ex vivo angiogenesis models. MGDG and SQDG suppressed microvessel growth in an ex vivo angiogenesis model using a rat aortic ring. The glycolipids inhibited human umbilical vein endothelial cell (HVUEC) tube formation on a reconstituted basement membrane and HUVEC proliferation. These results demonstrate that glycolipids from spinach would suppress tumor growth by suppressing angiogenesis and might be candidates for anti-cancer or anti-angiogenic materials.
Matsubara
Kiminori
K
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan. kmatsuba@fhw.oka-pu.ac.jp
Matsumoto
Hiroaki
H
Mizushina
Yoshiyuki
Y
Mori
Masaharu
M
Nakajima
Nobuyoshi
N
Fuchigami
Michiko
M
Yoshida
Hiromi
H
Hada
Takahiko
T
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000254:growth & development; D001808:Blood Vessels / Q000187:drug effects* / Q000254:growth & development; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D038983:Galactolipids / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D018724:Spinacia oleracea / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D038983:Galactolipids; D006017:Glycolipids; D010936:Plant Extracts; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; C013694:sulfoquinovosyl diglyceride