Musculin/MyoR is expressed in kidney side population cells and can regulate their function.
Musculin/MyoR is a new member of basic helix-loop-helix transcription factors, and its expression is limited to skeletal muscle precursors. Here, we report that musculin/MyoR is expressed in adult kidney side population (SP) cells and can regulate their function. SP phenotype can be used to purify stem cell-rich fractions. Microarray analysis clarified that musculin/MyoR was exclusively expressed in kidney SP cells, and the cells resided in the renal interstitial space. Musculin/MyoR-positive cells were decreased in acute renal failure, but infusion of kidney SP cells increased musculin/MyoR-positive cells and improved renal function. Kidney SP cells in reversible acute renal failure expressed a high level of renoprotective factors and leukemia inhibitory factor (LIF), but not in irreversible chronic renal failure. In cultured kidney SP cells, LIF stimulated gene expression of renoprotective factors, and down-regulation of musculin/MyoR augmented LIF-induced gene expression. Our results suggest that musculin/MyoR may play important roles not only in developmental processes but also in regenerative processes in adult tissue.
Hishikawa
Keiichi
K
Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan. hishikawa-tky@umin.ac.jp
Marumo
Takeshi
T
Miura
Shigeki
S
Nakanishi
Asato
A
Matsuzaki
Yumi
Y
Shibata
Katsunori
K
Ichiyanagi
Tomoko
T
Kohike
Hiroko
H
Komori
Takuya
T
Takahashi
Ichiro
I
Takase
Osamu
O
Imai
Naohiko
N
Yoshikawa
Masahiro
M
Inowa
Toshihiko
T
Hayashi
Matsuhiko
M
Nakaki
Toshio
T
Nakauchi
Hiromitsu
H
Okano
Hideyuki
H
Fujita
Toshiro
T
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D015850:Interleukin-6 / Q000378:metabolism / Q000494:pharmacology; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009124:Muscle Proteins; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D051437:Renal Insufficiency / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D009124:Muscle Proteins; C498449:Ostn protein, mouse; D014157:Transcription Factors