title: Human sorbin is generated via splicing of an alternative transcript from the ArgBP2 gene locus.
abstract: We demonstrate that the human sorbin polypeptide is generated via splicing of an alternative transcript from the ArgBP2 gene locus. Previous studies have demonstrated that the central 139 amino acid region of the porcine sorbin polypeptide exhibits 95% homology to part of the human ArgBP2 protein. Yet neither the sorbin N- nor C-terminus has been identified in ArgBP2 or any other protein to date. Using computational analysis, we locate the sorbin N- and C-termini in the human ArgBP2 gene locus, and demonstrate that they are spliced to the 5' and 3' ends of the 95% homologous region. In addition, several sequence anomalies were identified in the putative human sorbin cDNA (AF396457). Thus, a revised human sorbin nucleotide sequence is proposed.
authors.affiliation: Section on Molecular Neuroscience, National Institute of Mental Health, National Institute of Health, 36 Convent Drive, MSC 4090, 9000 Rockville Pike Bethesda, MD 20892-4090, USA.
authors.forename: Damon
authors.initials: D
authors.lastname: Hand
authors.forename: Lee E
authors.initials: LE
authors.lastname: Eiden
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins; D014158:Transcription, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018398:Homeodomain Proteins; D010455:Peptides; D016601:RNA-Binding Proteins; C490755:SORBS2 protein, human; C071218:sorbin peptide