title: Demonstration by PCR and DNA sequencing of Corynebacterium pseudodiphtheriticum as a cause of joint infection and isolation of the same organism from a surface swab specimen from the patient.
abstract: A case of infectious arthritis following arthroscopy is described. Real-time PCR, using universal bacterial primers targeting the 16S rRNA gene, and subsequent DNA sequencing of the PCR product demonstrated the presence of DNA from Corynebacterium pseudodiphtheriticum in the synovial fluid from the affected knee. Culture from a surface swab from the site of purulent discharge from the knee was initially reported as growing normal skin microbiota. Knowing the result of the DNA analysis, the specimen was re-examined and a diphtheroid bacterium was isolated. The DNA sequence of the isolated bacterium was identical to that of the DNA in the joint. The isolated bacterium was tested for susceptibility to relevant antibiotics. Demonstration and identification of bacterial DNA by PCR and gene sequencing may not by itself give information on important characteristics such as susceptibility to antibiotics of the infecting bacterium. The present case illustrates that the results obtained by the method can be used to isolate the relevant bacterium in culture from other sites and thereby characterize it. It furthermore demonstrates that C. pseudodiphtheriticum can cause severe arthritis when inoculated into joints.
authors.affiliation: Department of Clinical Microbiology, State Serum Institute, Copenhagen, Denmark 2Department of Orthopedic Surgery, Roskilde County Hospital, Koege, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Kemp
authors.affiliation: Department of Clinical Microbiology, State Serum Institute, Copenhagen, Denmark 2Department of Orthopedic Surgery, Roskilde County Hospital, Koege, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Holtz
authors.affiliation: Department of Clinical Microbiology, State Serum Institute, Copenhagen, Denmark 2Department of Orthopedic Surgery, Roskilde County Hospital, Koege, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Andresen
authors.affiliation: Department of Clinical Microbiology, State Serum Institute, Copenhagen, Denmark 2Department of Orthopedic Surgery, Roskilde County Hospital, Koege, Denmark.
authors.forename: J J
authors.initials: JJ
authors.lastname: Christensen
mesh_terms: D000368:Aged; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000382:microbiology*; D001182:Arthroscopy / Q000009:adverse effects; D003352:Corynebacterium / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005260:Female; D006801:Humans; D007719:Knee Joint; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology; D013582:Synovial Fluid / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S