title: [Survival and growth of Vibrio cholerae O139 inside Acanthamoeba].
abstract: OBJECTIVE
  To study the survival and growth of Vibrio cholerae inside the Acanthamoeba polyphage.
  
  
  METHODS
  Survival and growth of Vibro cholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope.
  
  
  RESULTS
  Viable O139 was observed inside the amoebal vacuoles in 24 hours. Vacuoles were filled with more bacteria along with the longer period of co-culture. The process of O139 infection with Amoebae would include uptake, formation of O139 vacuole, multiplication, trophozoites lysed and expel under electron microscopy. Some infected trophozoites could subsequently encyst and the surviving O139 could locate in the vesicles inside the cysts.
  
  
  CONCLUSIONS
  O139 might survive and multiply in the trophozoites and reside inside the cysts of Amoebae, suggesting that Acanthamoebae might serve as one of the environmental hosts of Vibro cholerae.
authors.affiliation: School of Public Health, Fudan University, Shanghai 200032, China. qwjiang@shmu.edu.cn
authors.forename: Qing-wu
authors.initials: QW
authors.lastname: Jiang
authors.forename: Qin-xue
authors.initials: QX
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Pei-song
authors.initials: PS
authors.lastname: Zhong
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jia-di
authors.initials: JD
authors.lastname: Tan
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Shao
authors.forename: Zi-hua
authors.initials: ZH
authors.lastname: Li
mesh_terms: D000048:Acanthamoeba / Q000254:growth & development* / Q000382:microbiology* / Q000648:ultrastructure; D000818:Animals; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D041242:Vibrio cholerae O139 / Q000254:growth & development* / Q000648:ultrastructure; D014867:Water / Q000469:parasitology
chemical_list: D003470:Culture Media; D014867:Water