title: Frequency, species and molecular characterization of oral Candida in hosts of different age in China.
abstract: BACKGROUND
  Research indicates that host age is a determining factor in yeast carriage. From the neonatal period, humans go through several dentition periods, and the emergence and substitution of teeth and changes in living habits greatly change the environment of the oral cavity, and therefore influence colonization by oral commensal organisms, certainly including Candida spp. No previous study of Candida carriage by different age groups divided by dentition has been reported. This study supplies data on the geographical specificity of C. albicans genotypic subgroup distribution.
  
  
  METHODS
  All test individuals came from a single geographical locale over a short period. Following mucosal swab sampling, CHROMagar Candida-yeast differential media were used to determine the frequency of carriage and species. All C. albicans strains were confirmed by PCR and PCR using primers reported to span a transposable intron region in the 25S rRNA gene was used to determine genotypic subgroups.
  
  
  RESULTS
  The results demonstrate that for the tested population, the frequency of Candida species and the distribution of C. albicans genotypic subgroups varied with age group. With increasing age, the frequency of C. albicans decreases, non-C. albicans yeasts increases; Genotypic subgroup A is the dominating strain in the oral cavities of healthy young individuals.
  
  
  CONCLUSIONS
  The influence of dentition substitution on oral yeast carriage was minor.
authors.affiliation: West China College of Stomatology, Sichuan University, Chengdu City, China.
authors.forename: Q G
authors.initials: QG
authors.lastname: Qi
authors.forename: T
authors.initials: T
authors.lastname: Hu
authors.forename: X D
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041221:Candida glabrata / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041022:Candida tropicalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D002681:China; D004251:DNA Transposable Elements / Q000235:genetics; D003818:Dentition, Mixed; D019229:Dentition, Permanent; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D007438:Introns / Q000235:genetics; D009055:Mouth / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D014094:Tooth, Deciduous
chemical_list: D004251:DNA Transposable Elements; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S