title: Identification of a new RTN3 transcript, RTN3-A1, and its distribution in adult mouse brain.
abstract: The Reticulon (RTN) family of proteins is thought to play important roles in the regulation of neuronal regeneration. In this study, we have identified a novel alternative splicing isoform of the RTN gene family, RTN3-A1, which contains an additional 2.3-kb exon. The transcripts of human and mouse RTN3-A1 (about 5.0 kb) were first discovered by database sequence mining and analysis, and verified by cloning and sequencing. Northern blot analysis of 16 human tissues with a common probe of RTN3 transcripts and a specific probe for RTN3-A1 demonstrated that human RTN3-A1 is expressed mainly in brain tissues with a weak expression in the skeletal muscle. With Western blot analysis, the expected 100-kDa RTN3-A1 protein was detected in mouse brain. In situ hybridization with a mouse RTN3-A1-specific cRNA probe revealed that the mouse RTN3-A1 mRNA was regionally expressed in the neurons of the cerebral cortex, hippocampus, hypothalamus, and cerebellum of the adult mouse brain. In contrast to the transcripts of RTN1 and RTN2, RTN3-A1 shares some significant similarity with RTN4-A in exon structure, tissue distribution, and brain expression profile. Since other reports have shown that RTN4-A inhibits neuronal outgrowth and restricts the plasticity of the central nervous system, we speculate that RTN3-A1 might play certain roles in the central nervous system.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 200433 Shanghai, P.R. China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Cai
authors.forename: Hexige
authors.initials: H
authors.lastname: Saiyin
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Pingzhao
authors.initials: P
authors.lastname: Zhang
authors.forename: Lisha
authors.initials: L
authors.lastname: Tang
authors.forename: Xinghua
authors.initials: X
authors.lastname: Pan
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D017398:Alternative Splicing / Q000502:physiology; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001923:Brain Chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000070798:Nogo Proteins; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D000070798:Nogo Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C476198:RTN3 protein, human; C000603199:RTN4 protein, human; C482720:Rtn3 protein, mouse; C000603201:Rtn4 protein, mouse