title: Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination.
abstract: The aim of this study was to determine the immunomodulatory effects of IL-12, IL-18 and CD154 (CD40 ligand, CD40L) in DNA-vaccination against the classical swine fever virus. Four recombinant plasmids were constructed including the CSFV coding region for the glycoprotein gp55/E2 alone or together with porcine IL-12, IL-18 or CD154 genes. Five groups of four pigs each were immunized intramuscularly (i.m.) three times with the respective constructs. The control group was inoculated with empty plasmid DNA. Eighteen days after the final immunization, the pigs were challenged with a lethal dose of CSFV strain Eystrup and monitored for a further 16 days. This study showed that co-delivery of IL-18 and CD154 induced an earlier appearance of serum antibodies, reduced B-cell deficiency after infection and protected pigs against a lethal CSFV infection. In contrast, co-delivery of IL-12 led to a reduced titer of neutralizing antibodies and protection against a lethal CSFV challenge in comparison to the other pigs and to pigs that were immunized with a gp55/E2 plasmid alone.
authors.affiliation: Institute of Immunology, Friedrich Loeffler Institute, Paul-Ehrlich-Strasse 28, 72076 Tübingen, Federal Republic of Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wienhold
authors.forename: Elisenda
authors.initials: E
authors.lastname: Armengol
authors.forename: Annette
authors.initials: A
authors.lastname: Marquardt
authors.forename: Christian
authors.initials: C
authors.lastname: Marquardt
authors.forename: Heiner
authors.initials: H
authors.lastname: Voigt
authors.forename: Mathias
authors.initials: M
authors.lastname: Büttner
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.forename: Eberhard
authors.initials: E
authors.lastname: Pfaff
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics*; D000818:Animals; D023201:CD40 Ligand / Q000235:genetics / Q000276:immunology; D006691:Classical Swine Fever / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology; D010957:Plasmids; D013552:Swine; D013997:Time Factors; D019444:Vaccines, DNA / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D006023:Glycoproteins; D020382:Interleukin-18; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; D023201:CD40 Ligand; D018664:Interleukin-12