Letter: collision-induced dissociation of peptide thioesters: influence of the peptide length on the fragmentation.
Five peptide thioesters of increasing length were fragmented under two processes, in-source and in- collision cell fragmentation, using an electrospray source coupled to a triple quadrupole. Comparison of their fragmentations was made in regard to the length. The two fragmentation conditions show that the peptide length has no influence on structural information and that the fragmentation efficiency is higher for the smallest peptides than for the longest. The particularity of these peptide thioesters consists on the neutral loss of ethanethiol. The absence of the a3 fragment ion and the presence of the (a3-17) ion on the CID mass spectra are noted.
Buré
Corinne
C
Boujard
Olivier
O
Bertrand
Marylène
M
Lange
Catherine
C
Delmas
Agnès F
AF
D004952:Esters / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry
D004952:Esters; D010455:Peptides; D013438:Sulfhydryl Compounds