One in vitro model for visceral adipose-derived fibroblasts in chronic inflammation.
One pathogenesis of the obesity-associated complications is that consistent with increased body fat mass, the elevation of adipose tissue-derived cytokines inflicts a low-grade chronic inflammation, which ultimately leads to metabolic disorders. Adipocytes and macrophages in visceral adipose (VA) have been confirmed to contribute to the chronic inflammation; however, the role of the resident fibroblasts is still unknown. We established one VA fibroblast cell line, termed VAFC. Morphological analysis indicated that there were large numbers of pits at the cell plasma membrane. In vitro VAFC cells promoted bone marrow cells to differentiate into macrophages and protected them from apoptosis in the serum-free conditions. Additionally, they also interfered in lymphocytes proliferation. On the basis of these results, this cell line might be an in vitro model for understanding the role of adipose-derived fibroblasts in obesity-associated chronic inflammation.
Yue
Gui-Ping
GP
Laboratory of Biochemistry, National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Du
Li-Rui
LR
Xia
Tao
T
He
Xian-Hui
XH
Qiu
Huan
H
Xu
Li-Hui
LH
Chen
Xiao-Dong
XD
Feng
Sheng-Qiu
SQ
Yang
Zai-Qing
ZQ
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D002908:Chronic Disease; D017931:DNA Primers; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D020869:Gene Expression Profiling; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009765:Obesity / Q000473:pathology
D017931:DNA Primers