title: [Effect of curcumin on expression of human low density lipoprotein receptors in Xenopus Laevis oocytes].
abstract: OBJECTIVE
  To investigate the molecular mechanism of curcumin in reducing blood lipids by establishing gene expression system of human low density lipoprotein receptors (LDL-R) in Xenopus Laevis oocytes (XLO).
  
  
  METHODS
  The expression of LDL-R on cytomembrane was determined using immuno-fluorescent, ligand-fluorescent and immune colloidal gold techniques after human LDL-R containing p3.7 LDL plasmid was led into nucleus. And the expression of LDL-R gene in XLO was quantitatively determined by ELISA after being interfered with different concentrations of curcumin.
  
  
  RESULTS
  The human LDL-R gene could be expressed on XLO, which could be significantly enhanced by curcumin in a dose-dependent manner. Conclusion One of the paths of curcumin in reducing blood lipids and anti-atherosclerosis was improving LDL-R gene expression and increasing the LDL-cholesterol absorption of cells.
authors.affiliation: Department of Life Science, Zhejiang College of Traditional Chinese Medicine, Hangzhou.
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Fan
authors.forename: Xing-de
authors.initials: XD
authors.lastname: Wo
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics; D014982:Xenopus laevis
chemical_list: D011973:Receptors, LDL; D003474:Curcumin