title: Characterization of purified c-type heme-containing peptides and identification of c-type heme-attachment sites in Shewanella oneidenis cytochromes using mass spectrometry.
abstract: We describe methods for mass spectrometric identification of heme-containing peptides from c-type cytochromes that contain the CXXCH (X=any amino acid) sequence motif. The heme fragment ion yielded the most abundant MS/MS peak for standard heme-containing peptides with one amino acid difference for both 2+ and 3+ peptide charge states; both sequence and charge affect the extent of heme loss. Application to Shewanella oneidenis demonstrated the utility of this approach for identifying c-type heme-containing peptides from complex proteome samples.
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Bogdan
authors.initials: B
authors.lastname: Bogdanov
authors.forename: Eric F
authors.initials: EF
authors.lastname: Strittmatter
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Vilkov
authors.forename: Marina
authors.initials: M
authors.lastname: Gritsenko
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.forename: Dwayne A
authors.initials: DA
authors.lastname: Elias
authors.forename: Shuisong
authors.initials: S
authors.lastname: Ni
authors.forename: Margaret
authors.initials: M
authors.lastname: Romine
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Pasa-Tolić
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis*; D001665:Binding Sites; D003574:Cytochrome c Group / Q000032:analysis*; D006418:Heme; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000302:isolation & purification; D020592:Shewanella / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D010446:Peptide Fragments; D006418:Heme