title: Monitoring dopamine release from single living vesicles with nanoelectrodes.
abstract: Carbon fiber nanoelectrodes (tip diameter = ca. 100 nm) have been first used to monitor real-time dopamine release from single living vesicles of single rat pheochromocytoma (PC12) cells. The experiments show that active and inactive release sites exist on the surface of cells, and the spatial distributions have been differentiated even in the same active release zone. It is first demonstrated that multiple vesicles can sequentially release dopamine at the same site of the cell surface, which possibly plays the main role in the dopamine release from PC12 cells.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Wen-Zhan
authors.initials: WZ
authors.lastname: Wu
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zong-Li
authors.initials: ZL
authors.lastname: Wang
authors.forename: Jie-Ke
authors.initials: JK
authors.lastname: Cheng
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Rong-Ying
authors.initials: RY
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002244:Carbon / Q000737:chemistry; D000077482:Carbon Fiber; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005089:Exocytosis / Q000502:physiology; D008839:Microelectrodes; D036103:Nanotechnology; D018377:Neurotransmitter Agents / Q000737:chemistry; D016716:PC12 Cells; D051381:Rats
chemical_list: D000077482:Carbon Fiber; D018377:Neurotransmitter Agents; D002244:Carbon; D004298:Dopamine