title: Production of a transgenic pig expressing human albumin and enhanced green fluorescent protein.
abstract: We introduced a fusion gene of human albumin and enhanced green fluorescent protein (EGFP) into porcine oocytes using the sperm vector method, and produced a piglet that showed clear expression of GFP in the hooves and skin. PCR and Southern blotting analysis of genomic DNA extracted from the piglet's tissues, including the liver, showed that the tissues carried the transgene. RT-PCR analysis demonstrated that both the human albumin and EGFP genes were expressed in the tissues. The fact that human albumin gene was integrated and expressed in the liver of the transgenic pig opened a way for us to achieve our goal, which was the use of transgenic pigs for the bioartificial liver support system.
authors.affiliation: Division of Artificial Organs & Transplantation, Department of Surgery, Faculty of Medicine, University of Tokyo, Japan. narusek-sur@h.u-tokyo.ac.jp
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Naruse
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Hiro-omi
authors.initials: HO
authors.lastname: Kawano
authors.forename: Hideto
authors.initials: H
authors.lastname: Ueda
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kurome
authors.forename: Koji
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Maiko
authors.initials: M
authors.lastname: Endo
authors.forename: Tohru
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagashima
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Makuuchi
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D002645:Chickens; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009865:Oocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D019076:Transgenes
chemical_list: D000418:Albumins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D004247:DNA