title: Determination of endocrine disruptors in environmental waters using poly(acrylamide-vinylpyridine) monolithic capillary for in-tube solid-phase microextraction coupled to high-performance liquid chromatography with fluorescence detection.
abstract: A method for the determination of endocrine disruptors, bisphenol A and 17alpha-ethinylestradiol, in environmental water samples was developed using in-tube solid-phase microextraction followed by liquid chromatography and fluorescence detection. A poly(acrylamide-vinylpyridine) monolithic capillary column was applied as the extraction media in view of its greater phase ratio than open-tubular capillaries and thus higher extraction efficiency. After optimizing the extraction conditions, bisphenol A and 17alpha-ethinylestradiol were extracted directly from water samples in a wide dynamic linear range of 0.5-1000 ng mL(-1), with the detection limits obtained as 0.064 and 0.12 ng mL(-1), respectively. The precision of the method was satisfactory with the intraday and interday RSD values smaller than 7.2%. Environmental water samples of different sources were successfully analyzed with the presented method and the monolithic capillary was proved to be robust and reusable in analyzing real water samples.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Shi-Lu
authors.initials: SL
authors.lastname: Da
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004702:Endocrine Glands / Q000187:drug effects; D008832:Microchemistry; D009641:Norethynodrel / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D009641:Norethynodrel; D010636:Phenols; D014874:Water Pollutants, Chemical; C002029:17alpha-ethynylestr-5(10)-ene-3alpha,17beta-diol; C006780:bisphenol A; D000098826:Bisphenol A Compounds