Effect of LLLT Ga-Al-As (685 nm) on LPS-induced inflammation of the airway and lung in the rat.
The purpose of this study was to investigate the effect of low level laser therapy (LLLT) on male Wistar rat trachea hyperreactivity (RTHR), bronchoalveolar lavage (BAL) and lung neutrophils influx after Gram-negative bacterial lipopolyssacharide (LPS) intravenous injection. The RTHR, BAL and lung neutrophils influx were measured over different intervals of time (90 min, 6 h, 24 h and 48 h). The energy density (ED) that produced an anti-inflammatory effect was 2.5 J/cm(2), reducing the maximal contractile response and the sensibility of trachea rings to methacholine after LPS. The same ED produced an anti-inflammatory effect on BAL and lung neutrophils influx. The Celecoxib COX-2 inhibitor reduced RTHR and the number of cells in BAL and lung neutrophils influx of rats treated with LPS. Celecoxib and LLLT reduced the PGE(2) and TXA(2) levels in the BAL of LPS-treated rats. Our results demonstrate that LLLT produced anti-inflammatory effects on RTHR, BAL and lung neutrophils influx in association with inhibition of COX-2-derived metabolites.
Aimbire
F
F
Laboratório de Farmacologia e LASER, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba Av: Shishima Hifumi 2911, São José dos Campos, S.P., 12240-000, Brazil. aimbire@univap.br
Albertine
R
R
de Magalhães
R G
RG
Lopes-Martins
R A B
RA
Castro-Faria-Neto
H C
HC
Zângaro
R A
RA
Chavantes
M C
MC
Pacheco
M T T
MT
D000818:Animals; D016535:Bronchial Hyperreactivity / Q000532:radiotherapy*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D000068579:Celecoxib; D002634:Chemotaxis, Leukocyte; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000032:analysis; D008070:Lipopolysaccharides; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009504:Neutrophils / Q000166:cytology; D011014:Pneumonia / Q000532:radiotherapy*; D011336:Probability; D011720:Pyrazoles / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D012016:Reference Values; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000494:pharmacology*; D014132:Trachea / Q000503:physiopathology
D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib; D015232:Dinoprostone