RESISTANCE TO FUSARIUM OXYSPORUM 1, a dominant Arabidopsis disease-resistance gene, is not race specific.
Arabidopsis thaliana ecotypes differ in their susceptibility to Fusarium wilt diseases. Ecotype Taynuilt-0 (Ty-0) is susceptible to Fusarium oxysporum forma specialis (f.) matthioli whereas Columbia-0 (Col-0) is resistant. Segregation analysis of a cross between Ty-0 and Col-0 revealed six dominant RESISTANCE TO FUSARIUM OXYSPORUM (RFO) loci that significantly contribute to f. matthioli resistance in Col-0 relative to Ty-0. We refer to the locus with the strongest effect as RFO1. Ty-0 plants in which only the Col-0 allele of RFO1 (RFO1(Col-0)) was introduced were resistant to f. matthioli. Surprisingly, RFO1(Col-0) also conferred resistance to f. raphani, demonstrating that RFO1-mediated resistance is not race specific. Expression of resistance by RFO2, RFO4, or RFO6 was dependent on RFO1(Col-0). Map-based cloning of RFO1(Col-0) showed that RFO1 is identical to the previously named Arabidopsis gene WAKL22 (WALL-ASSOCIATED KINASE-LIKE KINASE 22), which encodes a receptor-like kinase that does not contain an extracellular leucine-rich repeat domain. Consistent with these results, a Col-0 rfo1 loss-of-function mutant was more susceptible to f. matthioli, f. conglutinans, and f. raphani. Thus, RFO1 encodes a novel type of dominant disease-resistance protein that confers resistance to a broad spectrum of Fusarium races.
Diener
Andrew C
AC
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.
Ausubel
Frederick M
FM
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005670:Fusarium / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D005799:Genes, Dominant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D007178:Inbreeding; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D013045:Species Specificity
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C036483:T-DNA; D011494:Protein Kinases; C514341:RFO1 protein, Arabidopsis; D020156:Salicylic Acid