Human immunodeficiency virus type 1 KK26-27 matrix mutants display impaired infectivity, circularization and integration but not nuclear import.
We analyzed the role of human immunodeficiency virus (HIV)-1 matrix protein (MA) during the virus replication afferent phase. Single-round infection of H9 T lymphocytes showed that the combined mutation of MA Lys residues 26-27 in MA reported nuclear localization signal (NLS)-1 impaired infectivity, abrogated 2-LTR-circle formation and significantly reduced integration. However, the mutation did not affect viral DNA docking to chromatin in either interphasic or mitotic cells, indicating that MA N-terminal basic domain should not represent a major determinant of HIV-1 nuclear import in T lymphocytes. These data point to a previously unreported role of MA in the late, post-chromatin-binding, afferent phase of HIV-1 replication cycle.
Mannioui
Abdelkrim
A
UMR 7151 Centre National de la Recherche Scientifique-Université Paris 7, 75010 Paris, France. karim.mannioui@chu-stlouis.fr
Nelson
Elisabeth
E
Schiffer
Cecile
C
Felix
Nathalie
N
Le Rouzic
Erwann
E
Benichou
Serge
S
Gluckman
Jean Claude
JC
Canque
Bruno
B
D002453:Cell Cycle; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015683:Gene Products, gag / Q000235:genetics / Q000502:physiology*; D015488:HIV Antigens / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D009154:Mutation; D013601:T-Lymphocytes / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D016662:Virus Integration; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus
D002843:Chromatin; D004279:DNA, Viral; D015683:Gene Products, gag; D015488:HIV Antigens; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1