title: The yeast multicopper oxidase Fet3p and the iron permease Ftr1p physically interact.
abstract: High affinity iron uptake in yeast is carried out by a multicomponent system formed by the ferroxidase Fet3p and the iron permease Ftr1p. The currently accepted model predicts that Fet3p and Ftr1p are functionally associated, however, a structural interaction between these two proteins has not been proven yet. The methylotrophic yeast Pichia pastoris has been used to perform cross-linking studies aimed to demonstrate the existence of a Fet3p-Ftr1p complex. Cross-linking of membrane suspensions with the membrane-impermeable reagents DTSSP and BS(3) has evidenced the presence of a high molecular weight band with Fet3p oxidase activity. This band has been purified and subjected to N-terminal sequence analysis. Two sequences were found in the cross-linked species, one of which could be assigned to Fet3p and the other to Ftr1p. This is the first experimental demonstration that Fet3p and Ftr1p are physically associated.
authors.affiliation: Department of Biochemical Sciences A. Rossi Fanelli, University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy. mariacarmela.bonaccorsi@uniroma l.it
authors.forename: M Carmela
authors.initials: MC
authors.lastname: Bonaccorsi di Patti
authors.forename: Rossella
authors.initials: R
authors.lastname: Miele
authors.forename: M
authors.initials: M
authors.lastname: Eugenia Schininà
authors.forename: Donatella
authors.initials: D
authors.lastname: Barra
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D027682:Cation Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D002570:Ceruloplasmin / Q000737:chemistry* / Q000378:metabolism*; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism; D010843:Pichia / Q000201:enzymology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D027682:Cation Transport Proteins; D009097:Multienzyme Complexes; D002570:Ceruloplasmin