Enantioselective capillary electrophoretic separation of tryptophane- and tyrosine-methylesters in a dual system with a tetra-oxadiaza-crown-ether derivative and a cyclodextrin.
Different dual selector systems containing a cyclodextrin derivative (methyl-beta-cyclodextrin and dimethyl-beta-cyclodextrin) and a new diaza-crown-ether derivative (N-[2-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecan-7-yl)propanoyl]glycine) were studied in the enantioselective separation of tryptophan-methylester and tyrosine-methylester enantiomers. This paper deals with the systematic study of the effects of changing the composition of the background electrolyte on the resolution of the d- and l- forms using an experimental design approach. It was found that the dual systems allowed a better chiral separation of the amino acid derivatives. The experimental design approach also allowed improving the separation compared to the starting conditions (center point of the design), which were adopted from a previous study.
Elek
János
J
Research Group of Homogeneous Catalysis Hungarian Academy of Sciences, University of Debrecen, P.O. Box 7, Debrecen H-4010, Hungary.
Mangelings
Debby
D
Iványi
Timea
T
Lázár
István
I
Heyden
Y Vander
YV
D000465:Algorithms; D002021:Buffers; D020650:Combinatorial Chemistry Techniques; D047248:Crown Compounds / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D002021:Buffers; D047248:Crown Compounds; D003505:Cyclodextrins; D004573:Electrolytes; C503752:N-(2-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecan-7-yl)propanoyl)glycine; C040444:tyrosine methyl ester; D014443:Tyrosine; C028129:tryptophan methyl ester; D014364:Tryptophan; D005998:Glycine