title: Molecular epidemiology of rabies in northern Colombia 1994-2003. Evidence for human and fox rabies associated with dogs.
abstract: During the period 2000-2003, wild grey foxes (Urocyon cinereoargenteus) in northern Colombia became infected with rabies. In order to derive phylogenetic relationships between rabies viruses isolated in foxes, dogs and humans in this region, 902 nt cDNA fragments containing the G-L intergenic region and encoding the cytoplasmic domain of protein G and a fragment of protein L were obtained by RT-PCR, sequenced and compared. Phylogenetic analysis showed that rabies viruses isolated in foxes, dogs and humans belonged to a single genetic variant. Speculative analysis together with epidemiological data indicated that rabies in foxes may have been due to contact with rabid dogs. Rabies transmission between dogs, wild foxes and humans may happen in natural conditions in northern Colombia. This finding is the first to suggest dog-to-fox rabies transmission in South America, and provides another example of dog rabies variants being able to successfully colonize wildlife hosts.
authors.affiliation: Laboratorio de Virología, Instituto Nacional de Salud, Bogotá, Colombia. apaezm@ins.gov.co
authors.forename: A
authors.initials: A
authors.lastname: Páez
authors.forename: C
authors.initials: C
authors.lastname: Saad
authors.forename: C
authors.initials: C
authors.lastname: Núñez
authors.forename: J
authors.initials: J
authors.lastname: Bóshell
mesh_terms: D000818:Animals; D003105:Colombia / Q000453:epidemiology; D017931:DNA Primers; D004197:Disease Reservoirs; D004285:Dogs; D005589:Foxes; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D011818:Rabies / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012367:RNA, Viral