title: Molecular characterization of crane Coccidia, Eimeria gruis and E. reichenowi, found in feces of migratory cranes.
abstract: Eimeria gruis and E. reichenowi have lethal pathogenicity to a number of species of cranes. These parasites develop at multiple organs or tissues in infected cranes, thus lacking the specificity of infection sites shown by other Eimeria spp. in spite of morphologic similarity. To date, there have been many reports of crane Eimeria infections, however, genetic examinations of these parasites have never been published. In the present study, we isolated oocysts of E. gruis and E. reichenowi from crane feces at a wintering area in Japan. By phylogenic analysis, we first demonstrated that partial sequences of the isolates formed their own cluster, located separately from other Eimeria spp.
authors.affiliation: Department of Food and Nutrition, Osaka Yuhigaoka Gakuen Junior College, Tennoji-ku, Osaka 543-0073, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Takami
authors.forename: Niichiro
authors.initials: N
authors.lastname: Abe
authors.forename: Isao
authors.initials: I
authors.lastname: Kimata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tani
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sasai
authors.forename: Eiichiroh
authors.initials: E
authors.lastname: Baba
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D001715:Bird Diseases / Q000469:parasitology*; D001717:Birds / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology* / Q000662:veterinary*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004539:Eimeria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D007564:Japan; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D033661:Oocysts / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S