title: Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China.
abstract: OBJECTIVE
  To investigate the association between single nucleotide polymorphism (SNP) in promoter of the DNA methyltransferase 3B (DNMT3B) gene and risk for development and lymphatic metastasis of gastric cardiac adenocarcinoma (GCA).
  
  
  METHODS
  The hospital based case-control study included 212 GCA patients and 294 control subjects without overt cancer. The DNMT3B SNP was genotyped by PCR and restriction fragment length polymorphism (RFLP) analysis.
  
  
  RESULTS
  The C/C genotype was not detected in both GCA patients and controls. In control subjects, the frequency of T/T and C/T genotypes was 94.9% and 5.1% respectively, and that of T and C alleles was 97.4% and 2.6%, respectively. The genotype and allelotype distribution in the GCA patients was not significantly different from that in controls (P=0.34 and 0.33, respectively). When stratified by smoking status and family history of upper gastrointestinal cancer, significant difference in the genotype distribution was not observed between GCA patients and controls. The distribution of DNMT3B genotypes in GCA patients with or without lymphatic metastasis did not show significant difference (P=0.42).
  
  
  CONCLUSIONS
  The distribution of DNMT3B SNP in North China is distinct from that in Caucasians. Although this SNP has been associated with susceptibility to lung, head, neck and breast cancer, it may not be used as a stratification marker to predict susceptibility and lymphatic metastasis of GCA, at least in the population of North China.
authors.affiliation: Hebei Cancer Institute, Hebei Medical University, Jiankanglu 12, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Deng-Gui
authors.initials: DG
authors.lastname: Wen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Wei
authors.forename: Yu-Tong
authors.initials: YT
authors.lastname: He
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D006338:Heart Neoplasms / Q000201:enzymology / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics*; D044465:White People / Q000235:genetics; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B