Determination of glufosfamide in rat plasma by liquid chromatography/tandem mass spectrometry.
A sensitive and selective high-performance analytical method based on liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) was developed for the quantification of glufosfamide in rat plasma. Zidovudine was employed as internal standard. Glufosfamide was determined after methanol-mediated plasma protein precipitation using LC/MS/MS with an electrospray ionization interface in negative ion mode. Two sets of standard curves were developed, from 0.005 to 1.0 microg/mL and from 1.0 to 50.0 microg/mL. The assay was accurate (% deviations from nominal concentrations < 5%), precise and reproducible (intra- and inter-day coefficients of variation < 10%). Glufosfamide in rat plasma was stable over three freeze/thaw cycles, and at ambient temperatures, for at least 2 h. The validated method was successfully applied to the determination of glufosfamide plasma concentrations in rats for 24 h following an intravenous administration of 25 mg/kg.
Chen
Xiaoyan
X
Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang, China.
Sun
Yuming
Y
Cao
Xueqin
X
Jin
Fengdan
F
Zhong
Dafang
D
D000477:Alkylating Agents / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid; D005947:Glucose / Q000031:analogs & derivatives; D007069:Ifosfamide / Q000031:analogs & derivatives; D013058:Mass Spectrometry; D010752:Phosphoramide Mustards / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
D000477:Alkylating Agents; D010752:Phosphoramide Mustards; D012996:Solutions; C092028:beta-D-glucosylisophosphoramide mustard; D005947:Glucose; D007069:Ifosfamide