Application of ES cells for generation of respiration-deficient mice carrying mtDNA with a large-scale deletion.
In a previous study, we used mouse zygotes as recipients of mtDNA with a large-scale deletion mutation (DeltamtDNA) and generated respiration-deficient mice (mito-mice) carrying DeltamtDNA. In this study, we used mouse ES cells as recipients of DeltamtDNA, and generated mito-mice with DeltamtDNA only when the ES cells carried 17% DeltamtDNA. No chimera mice or their F(1) progenies were obtained from ES cells carrying more than 61% DeltamtDNA. These observations suggest that respiratory defects of ES cells inhibit their normal differentiation into chimera mice and mito-mice, and that ES cells are more effective than zygotes for generation of mito-mice carrying mtDNAs without significant pathogenic mutations.
Ishikawa
Kaori
K
Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan.
Kasahara
Atsuko
A
Watanabe
Naoki
N
Nakada
Kazuto
K
Sato
Akitsugu
A
Suda
Yoko
Y
Aizawa
Shinichi
S
Hayashi
Jun-Ichi
J
D000818:Animals; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D018014:Gene Transfer Techniques; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008928:Mitochondria / Q000235:genetics*; D028361:Mitochondrial Diseases / Q000196:embryology* / Q000235:genetics*; D012120:Respiration Disorders / Q000196:embryology / Q000235:genetics; D013234:Stem Cells
D004272:DNA, Mitochondrial