[Study on the culture of crown gall from Panax quinquefolium and the prouction of its secondary metabolites--Ginsenosides Re and Rg1].
It was clearly demonstrated that T-DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown gall in our experiment. This paper reported the influences of some kinds of physical-chemistry factors on the growth of crown gall of Panax quinquefolium and the production of its main active compounds--ginsenoside Re and ginsensoside Rg1. The results showed that White medium was the best one for ginsensoside Rg1 accumulation (0.095%) among the six media, but ginsensoside Re accumulation (0.194%) was the highest on the MS medium; The highest contents of ginsensoside Re (0.147%) and ginsensoside Rg1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5.6 for ginsensoside Rg1 synthesis(0.054%), and 5.8 for ginsensoside Re synthesis(0. 184% ); The contents of ginsensoside Re and ginsensoside Rg1 was the highest in the inoculum of 4 g and 2 g/flask (FW) respectively. The result also indicated that the concentration of inositol in 0.05 g/L could obviously promote ginsensoside Re synthesis (0.182%), and in 0.30 g/L for ginsensoside Rg1 (0.055%).
Yu
Rong-Min
RM
College of Pharmacy, Jinan University, Guangzhou 510632, China. rongminyu99@hotmail.com
Song
Yong-Bo
YB
Zhang
Hui
H
Ye
Wen-Cai
WC
Zhang
Yin-Lin
YL
Yao
Xin-Sheng
XS
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000502:physiology; D018929:Cell Culture Techniques; D036145:Ginsenosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000254:growth & development* / Q000378:metabolism* / Q000382:microbiology; D010941:Plant Tumors / Q000382:microbiology*
D036145:Ginsenosides; C049864:ginsenoside Re; C035054:ginsenoside Rg1