title: [Advances in analysis techniques of phosphoproteome].
abstract: In eukaryotes protein phosphorytion is a key event. By reversible protein phosphorylation eukaryotes control many cellular processes including signal transduction, gene expression, the cell cycle etc. Phosphoproteomics involves identification of phosphoproteins and phosphopeptides, localization of the exact residues that are phosphorylated and quantitation of phosphorylation. Because protein phosphorylation is a dynamic process, and it is present at low abundance within cells, and the phosphorylated sites on proteins might vary, and mass spectrometry (MS) signals from phosphopeptides are usually suppressed etc., so phosphoprotein analysis have more difficulties than nonphosphoprotein. In this article, we outline several analysis techniques for separation, identification and quantitation of phosphorylated proteins and peptides, and discuss the progress in these techniques. At present, MS is still an essential core identification technology for phosphoproteomic studies, To search better enrichment strategies are the main challenges in this rapidly evolving field. A major goal of quantitative proteomics is precise quantification and identification of proteins in complex mixtures. A common method for quantitative proteome analysis is the stable isotope labeling method. Today there is no single method that supersedes all others techniques for Phosphoproteomic studies. With continued development of sample preparation techniques and instrumentation, it should be possible to perform a global analysis of protein phosphorylation.
authors.affiliation: Department of Clinical Microbiology, Third Military Medical University, Chongqing 400038, China. W8301991@263.net
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Quan-Ming
authors.initials: QM
authors.lastname: Zou
authors.forename: Shao-Xi
authors.initials: SX
authors.lastname: Cai
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*
chemical_list: D010750:Phosphoproteins