title: Pharmacokinetic study of caffeic and rosmarinic acids in rats after oral administration.
abstract: p-Coumaric and ferulic acid are actively taken up by monocarboxylic acid transporter (MCT), whereas gallic acid, caffeic acid (CA), and rosmarinic acid (RA) are absorbed by paracellular diffusion in human intestinal Caco-2 cells, although CA has low affinity for MCT. We previously demonstrated that p-coumaric acid has a much higher absorption efficiency than gallic acid in rats, owing to the MCT-mediated absorption of p-coumaric acid in vivo (J. Agric. Food Chem. 2004, 52, 2527-2532). Here, absorption of orally administered CA and RA in rats has been studied to investigate their intestinal absorption characteristics and pharmacokinetics in vivo and to compare the results with those of p-coumaric and gallic acids obtained under identical conditions. Rats were given 100 micromol/kg body weight of CA and RA, and blood was collected from the portal vein and abdominal artery after administration. CA, RA, and their metabolites were quantified by a coulometric detection method using HPLC-ECD. The serum concentration of intact CA and RA in the portal vein peaked at 10 min after administration, with a C(max) of 11.24 micromol/L for CA and 1.36 micromol/L for RA. The area under the curve (AUC) for intact CA and RA in the portal vein was calculated from the serum concentration-time profile to be 585.0 and 60.4 micromol min L-1, respectively. The absorption efficiency of CA was about 9.7-fold higher than that of RA. Overall, the absorption efficiency of these compounds in vivo increases in the order: gallic acid = RA < CA < p-coumaric acid, which is in good agreement with results obtained in Caco-2 cells in vitro.
authors.affiliation: Applied Bioresearch Center, Research and Development Department, Kirin Brewery Company, Ltd., 3 Miyaharacho, Takasaki-shi, Gunma 370-1295, Japan. konishiy@kirin.co.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Konishi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hitomi
authors.forename: Michiko
authors.initials: M
authors.lastname: Yoshida
authors.forename: Eiji
authors.initials: E
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D002934:Cinnamates / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D053630:Depsides; D007408:Intestinal Absorption; D008297:Male; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D000097123:Rosmarinic Acid
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid; C040048:caffeic acid