[Cloning and sequence analysis of human uric acid transporter gene].
OBJECTIVE
To obtain full-length human urate transporter (hUAT) gene.


METHODS
Primers was designed according to the sequence of hUAT reported in Genbank. The target fragments were obtained by reverse transcriptional (RT) PCR from the mRNA extracted from human renal tubular epithelial cell lines (HK-2), followed by cloning into pEGFP-C1 plasmid. The cloned fragment was subjected to restriction mapping and sequence analysis for confirmation.


CONCLUSIONS
hUAT gene with correct sequence is successfully cloned from HK-2 cells. The restriction maps and sequence analysis of the selected clones were consistent with the sequence reported in Genbank.
Hong
Quan
Q
Center of Nephrology and Key Laboratory of PLA, General Hospital of the PLA, Beijing 100853, China. redhq@163.com
Wu
Di
D
Chen
Xiang-mei
XM
Hou
Kai
K
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014527:Uric Acid
D027361:Organic Anion Transporters; C103862:urate transporter; D014527:Uric Acid