Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators.
Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes a GRAS protein essential for Nod-factor signaling. NSP2 functions downstream of Nod-factor-induced calcium spiking and a calcium/calmodulin-dependent protein kinase. We show that NSP2-GFP expressed from a constitutive promoter is localized to the endoplasmic reticulum/nuclear envelope and relocalizes to the nucleus after Nod-factor elicitation. This work provides evidence that a GRAS protein transduces calcium signals in plants and provides a possible regulator of Nod-factor-inducible gene expression.
Kaló
Péter
P
Departments of Disease and Stress Biology and Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK.
Gleason
Cynthia
C
Edwards
Anne
A
Marsh
John
J
Mitra
Raka M
RM
Hirsch
Sibylle
S
Jakab
Júlia
J
Sims
Sarah
S
Long
Sharon R
SR
Rogers
Jane
J
Kiss
György B
GB
Downie
J Allan
JA
Oldroyd
Giles E D
GE
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008070:Lipopolysaccharides / Q000378:metabolism*; D029902:Medicago / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D016962:Sinorhizobium meliloti / Q000502:physiology*; D013559:Symbiosis; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D008070:Lipopolysaccharides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium