title: A single exposure to hyperbaric oxygen does not cause oxidative stress in isolated platelets: no effect on superoxide dismutase, catalase, or cellular ATP.
abstract: OBJECTIVE
  The aim of the study was to investigate whether a single hyperbaric oxygen exposure causes oxidative stress in isolated platelets.
  
  
  METHODS
  Isolated horse platelets were exposed to 100% oxygen at 2.2 atmospheres, or 100% oxygen under normobaric conditions, or air under normobaric conditions for 90 min.
  
  
  RESULTS
  There were no differences in platelet SOD activity between conditions, but there was a rise in SOD in all cases after 24 h (in control platelets at 24 h, SOD was 11.9 +/- 1.9 nmol/min/mg protein compared to initial background levels of 8.2 +/- 1.9 nmol/min/mg protein) (P < 0.05). Neither platelet catalase activity nor platelet GSH concentration changed over time, nor between conditions (catalase activity remained at around 12 units/mg protein, and GSH at around 1.58 nmol/mg protein).
  
  
  CONCLUSIONS
  These data suggest that a single HBO exposure has no detrimental effect on platelet biochemistry, and does not cause overt oxidative stress in vitro.
authors.affiliation: Institute of Biomedical and Clinical Science, Peninsula Medical School, Exeter, EX1 2LU, UK. frances.shaw@pms.ac.uk
authors.forename: Frances L
authors.initials: FL
authors.lastname: Shaw
authors.forename: Richard D
authors.initials: RD
authors.lastname: Handy
authors.forename: Phil
authors.initials: P
authors.lastname: Bryson
authors.forename: J Robert
authors.initials: JR
authors.lastname: Sneyd
authors.forename: A John
authors.initials: AJ
authors.lastname: Moody
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000097:blood / Q000187:drug effects*; D005978:Glutathione / Q000097:blood; D006736:Horses; D006931:Hyperbaric Oxygenation; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects*
chemical_list: D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione