[Study on the direct MAIPA technique in the differential diagnosis of immune and non-immune thrombocytopenia].
OBJECTIVE
To evaluate the clinical usefulness of direct monoclonal antibody immobilization of platelet antigen (MAIPA) technique in the differential diagnosis of immune and non-immune thrombocytopenia.


METHODS
Platelet-bound autoantibodies in thrombocytopenic patients (immune and non-immune) were measured by direct MAIPA. Monoclonal antibodies against GP II b/III a, GPIb and GP I a/II a were used.


RESULTS
The positive rates of platelet-bound GP-specific autoantibodies between immune (76.4%) and non-immune thrombocytopenia (3.6%) were significantly different (P < 0.05). The direct MAIPA had a sensitivity of 76.4%, a specificity of 96.4%, and a positive predictive value of 97.1% for the diagnosis of immune thrombocytopenia. There was a significant inverse correlation between platelet-bound GP II b/III a specific autoantibody levels and platelet counts (r = -0.338, P < 0.05).


CONCLUSIONS
The direct MAIPA technique can be used to differentiate immune from non-immune thrombocytopenias.
Qin
Ping
P
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Hou
Ming
M
Sun
Jian-zhi
JZ
Lu
Lu
L
Shi
Yan
Y
Zhu
Yuan-yuan
YY
Li
Li-zhen
LZ
Zhang
Mao-hong
MH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011696:Purpura, Thrombocytopenic / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D010980:Platelet Membrane Glycoproteins