An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor.
Apple flavor is characterized by combinations of ester compounds, which increase markedly during fruit ripening. The final step in ester biosynthesis is catalyzed by alcohol acyl transferases (AATs) that use coenzyme A (CoA) donors together with alcohol acceptors as substrates. The gene MpAAT1, which produces a predicted protein containing features of other plant acyl transferases, was isolated from Malus pumila (cv. Royal Gala). The MpAAT1 gene is expressed in leaves, flowers and fruit of apple. The recombinant enzyme can utilize a range of alcohol substrates from short to medium straight chain (C3-C10), branched chain, aromatic and terpene alcohols. The enzyme can also utilize a range of short to medium chain CoAs. The binding of the alcohol substrate is rate limiting compared with the binding of the CoA substrate. Among different alcohol substrates there is more variation in turnover compared with K(m) values. MpAAT1 is capable of producing many esters found in Royal Gala fruit, including hexyl esters, butyl acetate and 2-methylbutyl acetate. Of these, MpAAT1 prefers to produce the hexyl esters of C3, C6 and C8 CoAs. For the acetate esters, however, MpAAT1 preference depends upon substrate concentration. At low concentrations of alcohol substrate the enzyme prefers utilizing the 2-methylbutanol over hexanol and butanol, while at high concentrations of substrate hexanol can be used at a greater rate than 2-methylbutanol and butanol. Such kinetic characteristics of AATs may therefore be another important factor in understanding how the distinct flavor profiles of different fruit are produced during ripening.
Souleyre
Edwige J F
EJ
Molecular Olfaction Group, Mt Albert Research Centre, The Horticultural and Food Research Institute of New Zealand Ltd. (HortResearch), Auckland, New Zealand. esouleyre@hortresearch.co.nz
Greenwood
David R
DR
Friel
Ellen N
EN
Karunairetnam
Sakuntala
S
Newcomb
Richard D
RD
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D004952:Esters / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013649:Taste; D013696:Temperature; D014835:Volatilization
D004952:Esters; D010940:Plant Proteins; D000217:Acyltransferases; C411082:alcohol acyltransferase, Fragaria ananassa