title: [Development of a multiantigenic serological test for tuberculosis diagnosis].
abstract: OBJECTIVE
  The present work evaluated a multi-antigen printing immunoassay (MAPIA) for the serological diagnosis of tuberculosis.
  
  
  METHODS
  Sera were obtained from 66 patients with tuberculosis, verified clinically and bacteriologically and from 47 healthy individuals (control group). Sample sera were used for detection of antibodies against 3 enriched mixtures of proteins and 5 unique recombinant antigens. The antigens were presented in a solid matrix. Sensitivity, specificity and predictive values were evaluated and confirmed by a logistic regression analysis. A prevalence value was calculated and used for the selection of the best antigenic combination.
  
  
  RESULTS
  The sensitivity and specificity values of individual antigens varied between 5-83% and 9-100%. The enriched mixtures values were more accurate than those obtained with the recombinant antigens. Combinations of several antigens improved the sensitivity values up to the 81% level. In most cases, specificity values of 57% or less were obtained.
  
  
  CONCLUSIONS
  These results suggested that the multiantigenic test can be a useful screening tool, to be used in conjunction with the more definitive diagnostic tests.
authors.affiliation: Laboratorio de Micobacterias, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
authors.forename: Claudia Marcela
authors.initials: CM
authors.lastname: Castro
authors.forename: Tania Bibiana
authors.initials: TB
authors.lastname: Porras
authors.forename: Martha Inirida
authors.initials: MI
authors.lastname: Guerrero
authors.forename: Clara Ines
authors.initials: CI
authors.lastname: León
authors.forename: Maria Antonieta
authors.initials: MA
authors.lastname: Mojica
authors.forename: Martha
authors.initials: M
authors.lastname: Lara
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Parada
authors.forename: Clara
authors.initials: C
authors.lastname: Espitia
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial