[Frequency of prothrombin gene G20210A variant in the 3'-untranslated region in Zhuang ethnic Chinese].
OBJECTIVE
To ascertain the frequency of prothrombin (FII) gene 3'-untranslated region (3'-UT) G20210A variant and to explore whether this mutation is related to arterial thrombosis in Chinese Zhuang population.


METHODS
Seventy-six patients with cerebral thrombosis, 23 patients with myocardial infarction and 106 healthy Chinese Zhuang persons were studied. The G20210A mutant allele of the prothrombin gene in all blood specimens was investigated by DNA extraction, polymerase chain reaction amplification, Hind III digestion and polyacrylamide gel electrophoresis.


RESULTS
The patients and normal control subjects were all homozygous for the normal G20210G allele, and there was no FII G20210A variant.


CONCLUSIONS
Factor II gene 3'-UT G20210A mutant allele is absent in the 99 Chinese Zhuang ethnic patients with ischemic stroke and myocardial infarction and is absent in 106 normal healthy Zhuang people. FII G20210A mutation may not be a major risk factor for thrombogenesis in ethnic Chinese.
Chang
Jian-hua
JH
Department of Medical Oncology, Cancer Center of Sun Yat-sen University, Guangzhou, Guangdong, 510060, PR China. changjianhua@163.com
Ma
Yue
Y
Zhang
Guang-sen
GS
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002681:China; D004252:DNA Mutational Analysis; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D020767:Intracranial Thrombosis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009203:Myocardial Infarction / Q000235:genetics; D016133:Polymerase Chain Reaction; D011516:Prothrombin / Q000235:genetics*
D020413:3' Untranslated Regions; D011516:Prothrombin