Gender-specific association of insertion/deletion polymorphisms in the nogo gene and chronic schizophrenia.
Nogo is a myelin-associated protein associated with neurite outgrowth and regeneration. A previous study has reported an association between an insertion/deletion polymorphism in schizophrenia. We tested for the distribution of the polymorphism and haplotypes of this and another insertion/deletion polymorphism in our population. We have also developed an assay combining allele-specific polymerase chain reaction (AS-PCR) and restriction fragment length polymorphism (RFLP) to simultaneously type these two insertion/deletion polymorphisms. There was a statistically significant difference at the allelic level for both the CAA (chi2 = 4.378, df = 1, P value = 0.036) and TATC (chi2 = 5.807, df = 1, P = 0.016) polymorphisms in the female subgroup, but not in males. With our genotyping method, we also determined the molecular haplotype. Within the female gender, odds ratio is at 1.57 (95% CI 1.05-2.37) for CAACAA-TATC and 1.40 (95% CI 0.55-3.60) for CAA-TATC, the two at-risk haplotypes. Odds ratio is 0.63 (95% CI 0.42-0.93) for the protective wildtype haplotype CAA-TATCTATC. Further study of these two polymorphisms to investigate functional significance and confirm gender-specific association should be carried out.
Tan
Ene-Choo
EC
Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Kent Ridge, 117510, Singapore. tenechoo@dso.org.sg
Chong
Siow-Ann
SA
Wang
Hanhui
H
Chew-Ping Lim
Eileen
E
Teo
Yik-Ying
YY
D000328:Adult; D016009:Chi-Square Distribution; D002908:Chronic Disease; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009185:Myelin Proteins / Q000235:genetics* / Q000378:metabolism; D000070798:Nogo Proteins; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012559:Schizophrenia / Q000235:genetics*; D012727:Sex Characteristics
D009185:Myelin Proteins; D000070798:Nogo Proteins; D012333:RNA, Messenger; C000603199:RTN4 protein, human