title: The solution structure of an N-terminally truncated version of the yeast CDC24p PB1 domain shows a different beta-sheet topology.
abstract: Phox and Bem1 (PB1) domains mediate protein-protein interactions via the formation of homo- or hetero-dimers. The C-terminal PB1 domain of yeast cell division cycle 24 (CDC24p), a guanine-nucleotide exchange factor involved in cell polarity establishment, is known to interact with the PB1 domain occurring in bud emergence MSB1 interacting 1 (BEM1p) during the regulation of the yeast budding process via its OPR/PC/AID (OPCA) motif. Here, we present the structure of an N-terminally truncated version of the Sc CDC24p PB1 domain. It shows a different topology of the beta-sheet than the long form. However, the C-terminal part of the structure shows the conserved PB1 domain features including the OPCA motif with a slight rearrangement of helix alpha1. Residues which are important for the heterodimerization with BEM1p are structurally preserved.
authors.affiliation: Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, 13125 Berlin, Germany. leitner@fmp-berlin.de
authors.forename: Dietmar
authors.initials: D
authors.lastname: Leitner
authors.forename: Martin
authors.initials: M
authors.lastname: Wahl
authors.forename: Dirk
authors.initials: D
authors.lastname: Labudde
authors.forename: Gerd
authors.initials: G
authors.lastname: Krause
authors.forename: Anne
authors.initials: A
authors.lastname: Diehl
authors.forename: Peter
authors.initials: P
authors.lastname: Schmieder
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: Pires
authors.forename: Michele
authors.initials: M
authors.lastname: Fossi
authors.forename: Urs
authors.initials: U
authors.lastname: Wiedemann
authors.forename: Martina
authors.initials: M
authors.lastname: Leidert
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D017384:Sequence Deletion; D012996:Solutions / Q000737:chemistry
chemical_list: C073594:CDC24 protein, S cerevisiae; D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions