title: In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy.
abstract: The in situ hybridization kinetics of label-free DNA on mixed monolayers of peptide nucleic acid (PNA) and 6-mercapto-1-hexanol (MCH) on Au electrodes was investigated by electrochemical impedance spectroscopy (EIS) and used to discriminate the fully complementary DNA from the single-base mismatched hybrids.
authors.affiliation: Max-Planck-Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Liu
authors.forename: Shengjun
authors.initials: S
authors.lastname: Tian
authors.forename: Peter E
authors.initials: PE
authors.lastname: Nielsen
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D017097:Electric Impedance; D004563:Electrochemistry; D000441:Hexanols / Q000737:chemistry; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C503488:6-mercapto-1-hexanol; D018076:DNA, Complementary; D000441:Hexanols; D020135:Peptide Nucleic Acids; D013438:Sulfhydryl Compounds