Studies on soluble ectodomain proteins of relaxin (LGR7) and insulin 3 (LGR8) receptors.
The ectodomains of both the relaxin (LGR7) and the INSL3 (LGR8) receptors can be expressed on the cell surface using only a single transmembrane domain. These membrane-anchored proteins retain the ability to bind relaxin and can be cleaved from the cell surface. The subsequent LGR7 protein, 7BP, binds relaxin and can act as a functional relaxin antagonist. By contrast, the equivalent LGR8 protein 8BP does not bind relaxin or antagonize LGR8 activity. The 7BP protein has been successfully immobilized onto chemically derivatized surfaces for the capture of relaxin peptides and subsequent identification via SELDI-MS analysis.
Yan
Yan
Y
Howard Florey Institute, University of Melbourne, Victoria 3010, Australia.
Cai
Jin
J
Fu
Ping
P
Layfield
Sharon
S
Ferraro
Tania
T
Kumagai
Jin
J
Sudo
Satoko
S
Tang
Jian-Guo
JG
Giannakis
Eleni
E
Tregear
Geoffrey W
GW
Wade
John D
JD
Bathgate
Ross A D
RA
D002460:Cell Line; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism*; D018000:Receptors, Peptide; D012065:Relaxin / Q000378:metabolism*; D012995:Solubility
D007328:Insulin; C084797:Leydig insulin-like protein; D008565:Membrane Proteins; D011506:Proteins; C417543:RXFP1 protein, human; C472867:RXFP2 protein, human; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; D012065:Relaxin