Neomycin improves cationic lipid-mediated transfection of DNA in human cells.
Delivery of oligonucleotides has been a major impediment in the development of nucleic acid based drugs. In this report, we show that neomycin, an aminoglycoside antibiotic, when combined with a cationic lipid preparation such as DOTAP, enhances transfection efficiency of both reporter plasmids and oligonucleotides and results in a significant increase in transgene expression. The results described here open a new lead in ongoing efforts for oligonucleotide delivery.
Napoli
Sara
S
Laboratory of Experimental Oncology, Oncology Institute of Southern Switzerland (IOSI) Via Vela 6, 6500 Bellinzona, Switzerland.
Carbone
Giuseppina M
GM
Catapano
Carlo V
CV
Shaw
Nick
N
Arya
Dev P
DP
D002240:Carbohydrate Sequence; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009355:Neomycin / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D014162:Transfection / Q000379:methods*
D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D004247:DNA; D009355:Neomycin; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane