Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae.
Spores from the co-culture of Aspergillus foetidus and Rhizopus oryzae were subjected to UV, heat and NTG (3-nitro,5-methylguanidine) mutagenesis. A few colonies were screened from the selected media for tannase study. Amongst all, the best mutant isolated from the heat treatment (60 degrees C for 60 min) was SCPR 337. The maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 U/ml with an incubation period of 30 h as compared to wild strain where the incubation period was 48 h with an enzyme activity of 44.2 U/ml and gallic acid yield of 94%, respectively. The mutant was sensitive to tetracycline and was also an over-producer of protease and amylase.
Purohit
Jogeswar Satchidananda
JS
Microbial Biotechnology and Downstream Processing Laboratory, Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur 721 302, India.
Dutta
Jayati Ray
JR
Nanda
Ranjan Kumar
RK
Banerjee
Rintu
R
D000681:Amylases / Q000378:metabolism; D001230:Aspergillus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D018920:Coculture Techniques; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation; D005707:Gallic Acid / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D012233:Rhizopus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D013752:Tetracycline / Q000494:pharmacology; D014466:Ultraviolet Rays
D004798:Enzymes; D008769:Methylnitronitrosoguanidine; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D000681:Amylases; D013752:Tetracycline