[Screening of differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis].
OBJECTIVE
To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.


METHODS
A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastasis Hca-F and its synogenetic cell line Hca-P with low metastatic potential was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.


RESULTS
Ten differentially expressed cDNA fragments of Hca-F with high potential of lymphatic spreading were obtained, two of which were newly identified ones.


CONCLUSIONS
SSH is a useful technique to detect genes of differential expression and an effective method to clone novel genes.
Cui
Xiao-nan
XN
Department of Oncology, the 1st Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Tang
Jian-wu
JW
Hou
Li
L
Song
Bo
B
Liu
Ji-wei
JW
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger