Effect of amino acid substitutions on the candidacidal activity of LFampin 265-284.
LFampin 265-284, derived from bovine lactoferrin, has broad-spectrum antimicrobial activity against the yeast Candida albicans and several Gram-positive and Gram-negative bacteria. A glycine substitution scan was used to identify residues that are important for its candidacidal activity. Each single substitution of a positively charged residue led to considerable reduction in candidacidal activity, for each residue to a different extent. Substitution within the helix-facilitating N-terminal sequence DLIW had less severe effect; substitution of Ile and Trp led to a somewhat reduced potency. No substantial effects were found on the propensity to adopt a helical structure or to bind to C. albicans cells.
van der Kraan
Marieke I A
MI
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
van der Made
Christel
C
Nazmi
Kamran
K
van't Hof
Wim
W
Groenink
Jasper
J
Veerman
Enno C I
EC
Bolscher
Jan G M
JG
Nieuw Amerongen
Arie V
AV
D019943:Amino Acid Substitution; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; C508417:LFampin 265-284; D010446:Peptide Fragments; D010455:Peptides; D007781:Lactoferrin