The study of metabolic pathways in tumors based on the transcriptome.
DNA microarray technology revolutionized gene-expression analysis in molecular biology to observe patterns of gene expression in genomic scale. We review the biological aspects of genome-wide gene-expression activity in tumors specially focusing on the analysis of enzyme coding genes. First, the methods for analyzing gene-expression data for the study of metabolome in silico are discussed showing SV40T antigen expressing liver tumor data as an example. Next, an application for tumor metabolome analysis utilizing a reference set of gene-expression profiles is shown.
Bono
Hidemasa
H
Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan. bono@saitama-med.ac.jp
Okazaki
Yasushi
Y
D000818:Animals; D019295:Computational Biology; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D016678:Genome; D009363:Neoplasm Proteins / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000235:genetics*
D009363:Neoplasm Proteins