[Detection of CCAAT/enhancer binding protein alpha gene mutations in acute myeloid leukemia].
OBJECTIVE
To explore the relationship between CCAAT/enhancer binding protein alpha (C/EBPalpha) gene mutations and the development of acute myeloid leukemia (AML).


METHODS
The whole coding region of C/EBPalpha gene were screened in 48 cases of AML and 11 normal subjects by PCR-single strand conformation polymorphism (PCR-SSCP) and sequencing.


RESULTS
C/EBPalpha mutations were detected in 5 of 48 AML patients. Four duplications and 1 deletion were confirmed by DNA sequencing. All of those are newly identified mutations.


CONCLUSIONS
Different mutation types of C/EBPalpha gene exist in a small number of patients with AML and might be related to the pathogenesis of some leukemias.
Zhao
Jin-mei
JM
Institute of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China.
Wang
Hong-wei
HW
Xu
Zhi-fang
ZF
Zhu
Lei
L
Bai
Bo
B
Ge
Xiao-yan
XY
D000293:Adolescent; D000328:Adult; D000368:Aged; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics*; D002648:Child; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation
D022763:CCAAT-Enhancer-Binding Protein-alpha