Somatic embryogenesis from flower explants of cocoa (Theobroma cacao L.).
Two types of flower explants, staminoides and petals, were used for in vitro induction of somatic embryos in cocoa. After 14 days in culture, we observed globular structures and callus formation on both types of explants. However, the better results were obtained on staminoides: 98.3% formed callus and 86.2% somatic embryos on Murashige and Skoog (1962) medium supplemented with sucrose, coconut water, 2,4-D, kinetin and agar.
Silva
J J
JJ
Plant Biotechnology Study Centre, Granma University, AP 21, Bayamo, Granma, Cuba.
Debergh
P
P
D002099:Cacao / Q000196:embryology* / Q000502:physiology; D035264:Flowers / Q000254:growth & development; D066298:In Vitro Techniques; D012038:Regeneration; D012639:Seeds