In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy.
The in situ hybridization kinetics of label-free DNA on mixed monolayers of peptide nucleic acid (PNA) and 6-mercapto-1-hexanol (MCH) on Au electrodes was investigated by electrochemical impedance spectroscopy (EIS) and used to discriminate the fully complementary DNA from the single-base mismatched hybrids.
Liu
Jianyun
J
Max-Planck-Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany.
Tian
Shengjun
S
Nielsen
Peter E
PE
Knoll
Wolfgang
W
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D017097:Electric Impedance; D004563:Electrochemistry; D000441:Hexanols / Q000737:chemistry; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry
C503488:6-mercapto-1-hexanol; D018076:DNA, Complementary; D000441:Hexanols; D020135:Peptide Nucleic Acids; D013438:Sulfhydryl Compounds