title: Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae.
abstract: Spores from the co-culture of Aspergillus foetidus and Rhizopus oryzae were subjected to UV, heat and NTG (3-nitro,5-methylguanidine) mutagenesis. A few colonies were screened from the selected media for tannase study. Amongst all, the best mutant isolated from the heat treatment (60 degrees C for 60 min) was SCPR 337. The maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 U/ml with an incubation period of 30 h as compared to wild strain where the incubation period was 48 h with an enzyme activity of 44.2 U/ml and gallic acid yield of 94%, respectively. The mutant was sensitive to tetracycline and was also an over-producer of protease and amylase.
authors.affiliation: Microbial Biotechnology and Downstream Processing Laboratory, Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur 721 302, India.
authors.forename: Jogeswar Satchidananda
authors.initials: JS
authors.lastname: Purohit
authors.forename: Jayati Ray
authors.initials: JR
authors.lastname: Dutta
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Nanda
authors.forename: Rintu
authors.initials: R
authors.lastname: Banerjee
mesh_terms: D000681:Amylases / Q000378:metabolism; D001230:Aspergillus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D018920:Coculture Techniques; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation; D005707:Gallic Acid / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D012233:Rhizopus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D013752:Tetracycline / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D004798:Enzymes; D008769:Methylnitronitrosoguanidine; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D000681:Amylases; D013752:Tetracycline