Use of genotypic identification by sodA sequencing in a prospective study to examine the distribution of coagulase-negative Staphylococcus species among strains recovered during septic orthopedic surgery and evaluate their significance.
A total of 212 coagulase-negative Staphylococcus strains recovered prospectively during 119 surgeries for proven or suspected bone and joint infection (BJI) were identified by sodA sequencing. These strains were identified as 151 Staphylococcus epidermidis isolates, 15 S. warneri isolates, 14 S. capitis isolates, 9 S. hominis isolates, 6 S. lugdunensis isolates, 5 S. haemolyticus isolates, 4 S. caprae isolates, 4 S. pasteuri isolates, 3 S. simulans isolates, and 1 S. cohnii isolate. Only S. epidermidis, S. lugdunensis, S. capitis, and S. caprae were found to be infecting organisms and were involved, respectively, in 35 (81.4%), 3 (7.0%), 3 (7.0%), and 2 (4.6%) cases of BJI.
Sivadon
V
V
Laboratoire de Microbiologie, Hôpital Raymond Poincaré (AP-HP), 104 Bd Raymond Poincaré, 92380 Garches, France. valerie.sivadon-tardy@apr.ap-hop-paris.fr
Rottman
M
M
Chaverot
S
S
Quincampoix
J-C
JC
Avettand
V
V
de Mazancourt
P
P
Bernard
L
L
Trieu-Cuot
P
P
Féron
J-M
JM
Lortat-Jacob
A
A
Piriou
P
P
Judet
T
T
Gaillard
J-L
JL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001178:Arthroplasty / Q000009:adverse effects; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001850:Bone Diseases, Infectious / Q000382:microbiology / Q000601:surgery*; D003030:Coagulase / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D007592:Joint Diseases / Q000382:microbiology / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009985:Orthopedics / Q000379:methods; D011446:Prospective Studies; D012086:Reoperation; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D013482:Superoxide Dismutase / Q000235:genetics*
D001426:Bacterial Proteins; D003030:Coagulase; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase