title: Deletion of open reading frames 9, 10 and 11 from the avian adenovirus CELO genome: effect on biodistribution and humoral responses.
abstract: In this study, the in vivo effect of the 3.6 kbp deletion of the three open reading frames (ORF) 9, 10 and 11 found at the right end of the CELO genome was examined. Groups of chickens were inoculated oronasally with 10(5)-10(7) p.f.u. per animal of wild-type virus and two recombinant CELO strains (rCELO) expressing luciferase and secreted alkaline phosphatase (SEAP). The tissue biodistribution, assessed by PCR, was similar for both wild-type and recombinant viruses. The infectious viral particle titre was determined by a p.f.u. counting method and the antibody responses to the CELO vector and the SEAP antigen were evaluated by ELISA. Infectious particle titres in tissues from chickens inoculated with the wild-type CELO virus increased up to 6 days post-inoculation, and declined until 11 days while titres in organs from chickens inoculated with the rCELO strain were low and only detectable at 4 days post-inoculation. Moreover, although anti-CELO antibody levels were three times lower in sera from chickens inoculated with rCELO, antibodies directed to the heterologous SEAP antigen were detected. Based on these results, no differences in tropism were observed, but the level of production of viral particles and the humoral responses appeared to decrease. Viruses replicate less efficiently with a deletion performed at the right end of the CELO genome. Nevertheless, the presence of antibodies directed to heterologous antigens makes the CELO virus an advantageous candidate for avian vaccination.
authors.affiliation: Unité de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, Site Les Croix, BP 53, 22440 Ploufragan, France.
authors.forename: Frédérick
authors.initials: F
authors.lastname: Le Goff
authors.affiliation: Unité de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, Site Les Croix, BP 53, 22440 Ploufragan, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Méderlé-Mangeot
authors.affiliation: Unité de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, Site Les Croix, BP 53, 22440 Ploufragan, France.
authors.forename: André
authors.initials: A
authors.lastname: Jestin
authors.affiliation: Unité de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, Site Les Croix, BP 53, 22440 Ploufragan, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Langlois
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary / Q000821:virology; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D025681:Fowl adenovirus A / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D017353:Gene Deletion; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D016366:Open Reading Frames / Q000235:genetics*; D009928:Organ Specificity; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology; D011995:Recombination, Genetic; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D008156:Luciferases; D000469:Alkaline Phosphatase