Procedure for minimizing stress for fMRI studies in conscious rats.
Functional magnetic resonance imaging (fMRI) in conscious animals is evolving as a critical tool for neuroscientists. The present study explored the effectiveness of an acclimation procedure in minimizing the stress experienced by the animal as assessed by alterations in physiological parameters including heart rate, respiratory rate, and serum corticosterone levels. Results confirm that as the stress of the protocol is minimized, there is a significant decrease in head movements and enhancement in data quality. The feasibility of improving the quality of fMRI data acquired in alert rats by utilizing a relatively simple technique is presented.
King
Jean A
JA
Center for Comparative Neuroimaging, Department of Psychiatry, University of Massachusetts Medical Center, Worcester, MA 01655, USA. jean.king@umassmed.edu
Garelick
Timothy S
TS
Brevard
Mathew E
ME
Chen
Wei
W
Messenger
Tara L
TL
Duong
Timothy Q
TQ
Ferris
Craig F
CF
D000064:Acclimatization / Q000502:physiology*; D000223:Adaptation, Psychological / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001921:Brain / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000009:adverse effects / Q000523:psychology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control*
D003345:Corticosterone