[Construction of the scaffold model from heterogeneous small blood vessels].
OBJECTIVE
To establish a scaffold model from heterogeneous small blood vessels.


METHODS
Caudal arteries from 34 Wistar rats (average length 12.08 +/- 1.69 cm) were made into acellular blood vessel scaffolds. Some scaffolds were observed by electron microscope, and others were transplanted to the cut ends of ear central arteries of male Japanese big ear white rabbits.


RESULTS
Average external diameter was 0.74 +/- 0.08 mm in proximal, and 0.55 +/- 0.08 mm in distal end of rat caudal arteries. The small blood vessel scaffolds had shin wall which was white and soft, composed of fibrous tissues without cells. On the intima surface the fibrous tissues were arrayed densely in a grid-like pattern. After transplantation, the blood flow was reserved, and kept flowing freely in 24 hours. The pulsation of the transplanted artery was accessible and no blood leakage was found.


CONCLUSIONS
The natural scaffolds are composed of fibrous tissues, and can sustain the artery pulse pressure for 24 hours. It is better to suture the blood vessels by sleeve anastomosis.
Chen
Huayong
H
Department of Tissue Engineering, China Medical University, Shenyang Liaoning, 110001, PR China. chenhy6039@yahoo.com.cn
Bai
Shuling
S
Wang
Jun
J
D000818:Animals; D001158:Arteries / Q000637:transplantation; D001672:Biocompatible Materials; D019917:Blood Vessel Prosthesis Implantation; D005260:Female; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
D001672:Biocompatible Materials