Glutathione S-transferase M1, T1, and P1 genotypes and rheumatoid arthritis.
OBJECTIVE
To determine the effects of genetic polymorphisms of glutathione S-transferase (GST) M1, GSTT1, and GSTP on risk and severity of rheumatoid arthritis (RA) in a Korean population.


METHODS
A total of 258 patients with RA and 400 disease-free controls were enrolled. GST genotypes were determined by RFLP-PCR. HLA-DRB 1 typing and further subtyping of all alleles was performed using sequence-specific oligonucleotide probe hybridization after PCR. Severity of RA among cases was assessed by Steinbrocker anatomical stage. Risk was assessed by calculating the age and sex adjusted odds ratio (OR) and 95% confidence intervals (CI).


RESULTS
The OR for risk of RA with the GSTM1-null genotype was 1.40 (95% CI 1.02- 1.92, p = 0.04), and 1.86 (95% CI 1.12- 3.09, p = 0.005) among individuals without the shared epitope (SE). Among patients with RA, the OR for risk of severe RA for the GSTM1-null genotype was 2.45 (95% CI 1.04- 5.77, p = 0.02). No association was observed between the GSTT1 or GSTP1 genotypes and either risk or severity of RA.


CONCLUSIONS
These results suggest that the deletion polymorphism of GSTM1 is associated with increased susceptibility for RA, particularly among individuals who are not carriers of the HLA-DRB 1 SE.
Yun
Bo Ra
BR
Department of Internal Medicine, Division of Rheumatology, and the Hospital for Rheumatic Diseases, Hanyang University, Seoul, Korea.
El-Sohemy
Ahmed
A
Cornelis
Marilyn C
MC
Bae
Sang-Cheol
SC
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000235:genetics* / Q000503:physiopathology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007723:Korea; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012720:Severity of Illness Index
D000939:Epitopes; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D007527:Isoenzymes; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human