[Expression of proline-rich tyrosine kinase-2 (Pyk2) in gastric carcinoma and its significance].
OBJECTIVE
To investigate whether proline-rich tyrosine kinase-2 (Pyk2) is expressed differently in normal gastric mucosas and gastric carcinoma tissues and further to evaluate its significance.


METHODS
Expressions of Pyk2 in 59 cases of normal gastric mucosas and 52 cases of gastric carcinoma tissues were analysed by immunohistochemical methods.


RESULTS
Immunohistochemical studies showed that the positive rates of Pyk2 protein expression in normal gastric mucosas and gastric carcinoma tissues were 86.44% (51/59) and 19.23% (10/52) respectively. The difference between normal gastric mucosas and gastric carcinoma tissues was statistically significant (P<0.05). The positive rates of Pyk2 expression in highly differentiated gastric carcinoma and moderately/lowly differentiated gastric carcinoma were 47.37% (9/19) and 3.03% (1/33) respectively. Statistically significant differences (P<0.05) were observed in the levels of Pyk2 expression between highly differentiated gastric carcinoma and moderately/lowly differentiated gastric carcinoma. The positive rates of Pyk2 expression at different TNM stages gastric carcinoma were respectively: stage I 66.67% (6/9), stage II 30% (3/10), stage III 3.45% (1/29), stage IV 0% (0/4). The differences were statistically significant [(II+III+IV) v I, chi2=15.767, P<0.05].


CONCLUSIONS
In this study, we demonstrate that Pyk2 is expressed in normal gastric mucosas, whereas its expression declines significantly or almost disappears in gastric carcinoma tissues. The expression of Pyk2 progressively decreases with increasing grade of malignancy and TNM stages of gastric carcinoma. This phenomenon indicates that Pyk2 expression may be involved in the generation and development of gastric carcinoma.
Guo
Hong-jie
HJ
Department of General Surgery, Peking University First Hospital, Beijing 100034, China.
Wang
Xin
X
Liu
Yu-cun
YC
Wan
Yuan-lian
YL
Yin
Hong-fang
HF
Li
Ting
T
Zhu
Jing
J
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D051418:Focal Adhesion Kinase 2 / Q000096:biosynthesis* / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
D051418:Focal Adhesion Kinase 2