Melanin photoprotection in the human retinal pigment epithelium and its correlation with light-induced cell apoptosis.
Time-resolved electron paramagnetic resonance (TREPR) spectroscopy was used to study melanin free radicals in human retinal pigment epithelium (RPE) cells and tyrosine-derived synthetic melanin. TREPR signal traces from RPE cells reveal in vivo light-induced melanin free radical photochemistry in more detail than previously known. Electron spin polarization reflecting a non-Boltzmann population within the energy levels of the spin system is observed in RPE cells as the result of the triplet state photoproduction and subsequent disappearance of free radicals in the melanin polymer. In a set of RPE cells cultured from individual sources, differences in optical absorption, continuous wave EPR spectra, and TREPR signals were correlated with apoptosis assays performed by flow cytometry. Continuous wave EPR spectra of RPE cells and TREPR of acidified synthetic melanin suggest that increased melanin aggregation provides an increase in photoprotection in the RPE cells that are relatively less susceptible to blue light-induced apoptosis.
Seagle
Brandon-Luke L
BL
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Rezai
Kourous A
KA
Kobori
Yasuhiro
Y
Gasyna
Elzbieta M
EM
Rezaei
Kasra A
KA
Norris
James R
JR
D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D005333:Fetus; D006801:Humans; D008027:Light; D008543:Melanins / Q000494:pharmacology / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D011835:Radiation Protection
D008543:Melanins