title: World Health Organization grade 'a' motility and zona-binding test accurately predict IVF outcome for mild male factor and unexplained infertilities.
abstract: BACKGROUND
  The aim of this study was to determine the pronostic value of a sperm-zona pellucida (ZP) binding assay, combined with World Health Organization (WHO) grade 'a' sperm motility on the day of the IVF attempt, to predict sperm fertilizing ability in unexplained and moderate male factor infertilities.
  
  
  METHODS
  In total, 84 couples (64 unexplained infertility; 20 male factor) underwent both a sperm-ZP binding assay and an IVF attempt, irrespective of the test's result. The test was negative when grade 'a' motility was #5% and/or the ZP binding index was <0.7. Fertilization and pregnancy rates were related to the test's results.
  
  
  RESULTS
  Thirty-one patients had a negative test (group N) and 53 a positive test (group P). A difference was observed concerning the fertilization rate [median (range): 0 (0-75%) versus 50 (0-100%); P = 0.0001] and the number of cycles with fertilization rate <20% (65 versus 23%; P = 0.0002) between groups N and P respectively. In the group of unexplained and male factor infertilities, the test showed a sensitivity of 83 and 60%, specificity of 50 and 90%, positive predictive value of 76 and 86%, and negative predictive value of 61 and 69% respectively.
  
  
  CONCLUSIONS
  Sperm-ZP binding test, combined with WHO grade 'a' motility assessment, is relevant to prevent IVF fertilization failures in unexplained infertility and, most particularly, in moderate male factor infertility.
authors.affiliation: Service d'Histologie-Embryologie-Cytogénétique, Laboratoire de Biologie de la Reproduction, Assistance Publique - Hôpitaux de Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Sifer
authors.forename: T
authors.initials: T
authors.lastname: Sasportes
authors.forename: V
authors.initials: V
authors.lastname: Barraud
authors.forename: C
authors.initials: C
authors.lastname: Poncelet
authors.forename: J
authors.initials: J
authors.lastname: Rudant
authors.forename: R
authors.initials: R
authors.lastname: Porcher
authors.forename: I
authors.initials: I
authors.lastname: Cedrin-Durnerin
authors.forename: B
authors.initials: B
authors.lastname: Martin-Pont
authors.forename: J N
authors.initials: JN
authors.lastname: Hugues
authors.forename: J P
authors.initials: JP
authors.lastname: Wolf
mesh_terms: D000328:Adult; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007246:Infertility / Q000175:diagnosis / Q000628:therapy*; D007248:Infertility, Male / Q000175:diagnosis / Q000628:therapy*; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D018456:Reproductive Medicine / Q000379:methods; D012661:Semen / Q000378:metabolism; D012680:Sensitivity and Specificity; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D016896:Treatment Outcome; D014944:World Health Organization; D015044:Zona Pellucida / Q000473:pathology