Functional architecture of the mammalian striatum: mouse vascular and striosome organization and their anatomic relationships.
To determine whether the general architecture of striatal vessels and mu opioid receptor-rich striosomes is similar, we investigated 3D reconstructions of coronal sections in 10 FVB mice. The sections were stained for striosomes using a mu opioid receptor antibody (MOR1). We used computerized procedures to detect striosomes and vessels and to calculate volume, number and colocalization of striosomes and vessels. The results showed a lattice-like pattern of striosomes similar to, and often surrounding, blood vessels. Furthermore, co-localization calculations suggested that the striosomes are more vascular than the matrix. Vessel volume was 5.0+/-1.3% per microm3 in striosomes versus 3.6+/-0.9%microm3 in matrix (p=0.01). The findings emphasize the probable importance of a grid- or lattice-like structure as an organizing principle of striatal anatomy and function. In addition, the greater vascularity of the striosomes compared to the matrix suggests a unique function of this compartment in relation to humoral signals and neurotropic drugs.
Breuer
O
O
Faculty of Health Sciences, Ben Gurion University of the Negev, Israel.
Lawhorn
C
C
Miller
T
T
Smith
D M
DM
Brown
L L
LL
D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D017072:Neostriatum / Q000098:blood supply* / Q000166:cytology*; D009434:Neural Pathways / Q000033:anatomy & histology*