[Osteopontin expression and its relation to invasion and metastases in gastric cancer].
OBJECTIVE
To investigate the correlation between expression of the osteopontin (OPN) and invasion and metastases in gastric cancer.


METHODS
The expression of OPN, NF-kappaB p65 and matrix metallo-proteinase 9 (MMP-9) was detected by immunohistochemistry in non-cancer gastric tissue (n = 12 cases) and gastric cancer tissue (n = 72 cases).


RESULTS
(1) OPN, NF-kappaB p65 and MMP-9 were not expressed in 12 non-cancer gastric tissue samples(group A). Their expression rates were 43.3%, 40.0% and 46.7% respectively in 30 gastric cancer samples without lymph nodes metastasis (group B), but they increased to 76.9%, 73.1% and 80.8% in 26 gastric cancer samples with lymph nodes metastases (group C), and 87.5%, 81.3% and 93.8% respectively in 16 gastric cancer samples with lymph node and distant metastases (group D). (2) There were statistically significant differences in their expressions between group D and group B (P(a) = 0.004, P(c) = 0.007, P(e) = 0.002), and between group C and group B (P(b) = 0.011, P(d) = 0.013, P(f) = 0.009). (3) Despite some differences in positive expression rates, correlations existed between OPN and NF-kappaB p65, and between NF-kappaB p65 and MMP-9 (P(1) = 0.042, P(2) = 0.013; r(1)= 0.67, r(2)= 0.72).


CONCLUSIONS
Osteopondin espression is closely related to the invasion and metastases of gastric cancer. It may upregulate the expression of metastasis-related molecule MMP-9 by activating NF-kappaB pathway.
Zhang
Dong-tao
DT
The Key Laboratory of Bio-technology, Life Science School of Ningxia University, Yinchuan 750021, China.
Yuan
Jing
J
Yang
Li
L
Guo
Xin-ning
XN
Hao
Zhi-ming
ZM
Han
Zhe-yi
ZY
Wu
Kai-chun
KC
Fan
Dai-ming
DM
D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051996:Transcription Factor RelA / Q000378:metabolism*
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D051996:Transcription Factor RelA; D053495:Osteopontin; D020780:Matrix Metalloproteinase 9