title: Simultaneous quantification of stavudine, lamivudine and nevirapine by UV spectroscopy, reverse phase HPLC and HPTLC in tablets.
abstract: In the present study, simultaneous quantification of stavudine (SV), lamivudine (LV) and nevirapine (NV) in tablets by UV spectroscopy, reverse phase HPLC (RP-HPLC) and HPTLC methods were developed. In the UV multi-component spectral method, SV, LV and NV was quantified at 266, 271 and 315 nm, respectively. In the RP-HPLC method, the drugs were resolved using a mobile phase of 20 mM sodium phosphate buffer (containing 8 mM 1-octanesulphonicacid sodium salt):acetonitrile (4:1, v/v) with pH adjusted to 3.5 using phosphoric acid on a C18-ODS-Hypersil (5 microm, 250 mm x 4.6 mm) column in isocratic mode. The retention time of SV, LV and NV was 2.85, 4.33 and 8.39 min, respectively. In the HPTLC method, the chromatograms were developed using a mobile phase of chloroform:methanol (9:1, v/v) on precoated plate of silica gel 60 F254 and quantified by densitometric absorbance mode at 265 nm. The Rf of SV, LV and NV were 0.21-0.27, 0.62-0.72 and 0.82-0.93, respectively. Recovery values of 99.16-101.89%, percentage relative standard deviation of <0.7 and correlation coefficient (linear dynamic range) of 0.9843-0.9999 shows that the developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing SV, LV and NV.
authors.affiliation: Department of Pharmaceutical Analysis and Chemistry, C. L. Baid Metha College of Pharmacy, Old Mahabalipuram Road, Jyothi Nagar, Thorapakkam, Chennai 600096, India. anbu_azhagan@yahoo.com
authors.forename: Sockalingam
authors.initials: S
authors.lastname: Anbazhagan
authors.forename: Narayanareddy
authors.initials: N
authors.lastname: Indumathy
authors.forename: Pitchaimuthu
authors.initials: P
authors.lastname: Shanmugapandiyan
authors.forename: Seshaiah Krishnan
authors.initials: SK
authors.lastname: Sridhar
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D019380:Anti-HIV Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D006863:Hydrogen-Ion Concentration; D019259:Lamivudine / Q000032:analysis* / Q000737:chemistry; D019829:Nevirapine / Q000032:analysis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D018119:Stavudine / Q000032:analysis* / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013607:Tablets; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000097:Acetonitriles; D019380:Anti-HIV Agents; D010756:Phosphoric Acids; D018894:Reverse Transcriptase Inhibitors; D013451:Sulfonic Acids; D013607:Tablets; D019259:Lamivudine; D019829:Nevirapine; D012964:Sodium; D018119:Stavudine; C030242:phosphoric acid; C032159:acetonitrile