title: Analysis of gene expression in non-regressed and regressed bovine corpus luteum tissue using a customized ovarian cDNA array.
abstract: The lifespan of the bovine corpus luteum (CL) is an important factor in the control of normal ovarian cyclicity and the establishment and maintenance of pregnancy. There is increasing evidence that CL lifespan is regulated by alternative expression of genes that promote or inhibit luteolysis. To gain further insights into these events a 434 character ovarian cDNA array comprising genes attributed to key aspects of CL function including more than 100 anonymous expressed sequence tags (ESTs) was constructed and screened with alpha(33)P dATP labeled RNA isolated from non-regressed (n=6) and regressed (n=6) CL tissue. Significance analysis of microarrays (SAM) identified 15 genes that changed expression 1.7-fold or more with a false discovery rate of <5%. The differentially expressed genes encoded enzymes involved in steroid biosynthesis and oxygen radical metabolism and proteins involved in extracellular matrix remodeling, apoptosis and cell structure. Results for five of the differentially expressed genes including matrix gla protein and collagen alpha1(I) (extracellular matrix), glutathione-S-transferase alpha I (oxygen metabolism), clusterin (apoptosis) and scavenger receptor BI (steroid biosynthesis) were confirmed by Northern blot analysis and found to be significantly different (P<0.01) between non-regressed and regressed CL tissue. Collectively this study identified genes with recognized roles in CL regression, genes with potential roles in this process and genes whose function have yet to be defined in this event.
authors.affiliation: Animal Reproduction Department, Teagasc, Agriculture and Food Development Authority, Athenry, Galway, Ireland.
authors.forename: Orla M
authors.initials: OM
authors.lastname: Casey
authors.forename: Dermot G
authors.initials: DG
authors.lastname: Morris
authors.forename: Richard
authors.initials: R
authors.lastname: Powell
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Sreenan
authors.forename: Richard
authors.initials: R
authors.lastname: Fitzpatrick
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D018955:CD36 Antigens / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics; D002417:Cattle / Q000378:metabolism*; D051152:Clusterin / Q000235:genetics; D024042:Collagen Type I / Q000235:genetics; D003338:Corpus Luteum / Q000737:chemistry / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary*; D005982:Glutathione Transferase / Q000235:genetics; D003341:Luteolysis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D010053:Ovary / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D000097568:Matrix Gla Protein
chemical_list: D018955:CD36 Antigens; D002135:Calcium-Binding Proteins; D051152:Clusterin; D024042:Collagen Type I; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D005982:Glutathione Transferase; D012333:RNA, Messenger; D000097568:Matrix Gla Protein