title: Acute and long-term effects of IL-6 on cultured dorsal root ganglion neurones from adult rat.
abstract: IL-6 contributes to pain and hyperalgesia in inflamed tissue. We have investigated short- and long-term effects of IL-6 on dorsal root ganglion (DRG) neurones. Glycoprotein 130-like immunoreactivity (the signal transduction receptor subunit) was found in almost all neurones in DRG sections and in cultured DRG neurones from adult rat. In calcium-imaging studies bath application of IL-6 caused an increase of intracellular calcium in about one-third of the DRG neurones suggesting functional IL-6 receptors in a proportion of neurones. Long-term but not short-term exposure of DRG neurones to IL-6 in vitro significantly enhanced the proportion of DRG neurones expressing neurokinin 1 receptor-like immunoreactivity from 10% to up to 40%. This up-regulation was dependent on the activation of mitogen-activated protein kinase kinase (MEK) in the neurones, suggesting that the mitogen-activated protein kinase (MAPK) pathway is important for this effects of IL-6. Calcium-imaging studies demonstrated that previous exposure of DRG neurones to IL-6 enhanced the proportion of neurones that exhibit a substance P-induced rise in intracellular calcium. These data show that IL-6 has short- and long-term effects on a proportion of DRG neurones. These effects are likely to contribute to pro-nociceptive effects of IL-6.
authors.affiliation: Institute of Physiology, University of Jena, Germany.
authors.forename: Gisela Segond
authors.initials: GS
authors.lastname: von Banchet
authors.forename: Michaela
authors.initials: M
authors.lastname: Kiehl
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Schaible
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015850:Interleukin-6; D018040:Receptors, Neurokinin-1; D015232:Dinoprostone; D002118:Calcium