title: DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles.
abstract: Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functionality of these sequences was demonstrated by in vitro import assays into isolated organelles. Transient expression of GFP fusion proteins in protoplasts and onion epidermal cells confirmed the localisation of these proteins in plastids or mitochondria, respectively. The possession of organellar targeting sequences seems to be conserved among Why proteins of higher plant species, including potato p24.
authors.affiliation: Institute of Botany, University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Krause
authors.forename: Isabell
authors.initials: I
authors.lastname: Kilbienski
authors.forename: Maria
authors.initials: M
authors.lastname: Mulisch
authors.forename: Anja
authors.initials: A
authors.lastname: Rödiger
authors.forename: Anke
authors.initials: A
authors.lastname: Schäfer
authors.forename: Karin
authors.initials: K
authors.lastname: Krupinska
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism*; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000145:classification / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D015388:Organelles / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C503333:Whirly1 protein, Arabidopsis; C503334:Whirly2 protein, Arabidopsis; C503335:Whirly3 protein, Arabidopsis