Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes.
The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39.1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named GECCO. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses.
van Oers
Monique M
MM
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
Abma-Henkens
Marleen H C
MHC
Greenomics, Plant Research International BV, Wageningen, The Netherlands.
Herniou
Elisabeth A
EA
Department of Biological Sciences, Imperial College London, UK.
de Groot
Joost C W
JCW
Applied Bioinformatics, Plant Research International BV, Wageningen, The Netherlands.
Peters
Sander
S
Greenomics, Plant Research International BV, Wageningen, The Netherlands.
Vlak
Just M
JM
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
D000818:Animals; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; D004255:Deoxyribodipyrimidine Photo-Lyase