Embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins towards the sea urchin embryo cells.
The embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins on sea urchin (Lytechinus variegatus) embryo cells was investigated. IC50 doses for toxicity on larvae development varied from 0.6 up to 96.3 microg ml(-1) and these effects were largely reversed by previously heating the proteins. Changes in the glycoconjungate status of the cell surface were assessed by time-course binding of the proteins during embryogenesis according to their carbohydrate-binding specificity. Glucose/mannose binding-proteins bound embryo cells at the same stage of development, at a similar stage to the N-acetylglucosamine/N-acetylneuraminic acid binding-protein (WGA) and earlier than galactose specific ones. FITC-conjugates of these proteins confirmed the above results and revealed the presence of specific and differential receptors for them. Inhibition assays using inhibitory glycoproteins significantly diminished the labelled patterns of FITC-conjugates. In conclusion, the assayed proteins exhibited embryotoxicity and their binding requirements were useful for following changes in the pattern of cell surface glycoconjugates on embryo cells of sea urchin. This property could be useful in analyzing other cell types.
Macedo
Nívea M R
NM
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza-Ceará, CEP 60451-970, Brazil.
Costa-Lotufo
Letícia V
LV
Wilke
Diego V
DV
Bomfim
Liezelotte R
LR
Pessoa
Cláudia
C
Moraes
Manoel O
MO
Ramos
Márcio V
MV
D000117:Acetylglucosamine / Q000737:chemistry; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D020128:Inhibitory Concentration 50; D037102:Lectins / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D037601:Mannose-Binding Lectin / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D011485:Protein Binding; D012617:Sea Urchins; D013696:Temperature; D013997:Time Factors
D002241:Carbohydrates; D006001:Glycoconjugates; D006023:Glycoproteins; D037102:Lectins; D037601:Mannose-Binding Lectin; D010940:Plant Proteins; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose; D008358:Mannose; D000117:Acetylglucosamine