title: New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides.
abstract: A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the [M--H](-) ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moiety.
authors.affiliation: CNR-Istituto di Chimica e Tecnologia dei Polimeri, Viale Regina Margherita 6, 95123 Catania, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003640:Dealkylation; D007202:Indicators and Reagents; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007202:Indicators and Reagents; D008050:Lipid A; D008070:Lipopolysaccharides