title: CpG oligodeoxynucleotides stimulate cord blood mononuclear cells to produce immunoglobulins.
abstract: CpG oligodeoxynucleotides (ODNs) stimulate adult B cells leading to cellular proliferation and immunoglobulin production. It is unknown if CpG-ODNs similarly stimulate neonatal human B cells. Neonates have immature immune responses and are poorly responsive to thymus independent antigens such as polysaccharides. We determined umbilical cord cells' response to CpG-ODNs. Adult and umbilical cord B cells produced similar amounts of IgM (adult 1371 +/- 352 vs. cord 1873 +/- 1084 ng/ml) in response to CpG-ODN stimulation. Although CpG-ODN was able to stimulate adult IgG and IgA production, cord cells produced less IgG (153 +/- 58 vs. 10 +/- 2.5 ng/ml) and no detectable IgA upon CpG-ODN stimulation. CpG ODN stimulated IgM production from adult CD27-negative B cells which may account for their ability to stimulate the mostly naïve cord B cells. The polyclonal IgM response included pneumococcal polysaccharide antigen-specific antibodies. CpG-ODNs may be useful as neonatal vaccine adjuvants for polysaccharide antigens that are otherwise non-immunogenic.
authors.affiliation: Department of Pediatrics, Division of Pediatric Critical Care, 800 Rose Street, Room MN478, University of Kentucky, Lexington, KY 40536, USA. cdland2@uky.edu
authors.forename: Cheri D
authors.initials: CD
authors.lastname: Landers
authors.forename: Subbarao
authors.initials: S
authors.lastname: Bondada
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000328:Adult; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D022242:Pneumococcal Vaccines / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; C408982:CPG-oligonucleotide; D007136:Immunoglobulins; D009838:Oligodeoxyribonucleotides; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial