title: Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.
abstract: We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems.
authors.affiliation: Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Universitat Autònoma de Barcelona, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Durany
authors.forename: Philip
authors.initials: P
authors.lastname: Bassett
authors.forename: Amanda M E
authors.initials: AM
authors.lastname: Weiss
authors.forename: Rocky M
authors.initials: RM
authors.lastname: Cranenburgh
authors.forename: Pau
authors.initials: P
authors.lastname: Ferrer
authors.forename: Josep
authors.initials: J
authors.lastname: López-Santín
authors.forename: Carles
authors.initials: C
authors.lastname: de Mas
authors.forename: Julian A J
authors.initials: JA
authors.lastname: Hanak
mesh_terms: D000446:Aldehyde-Lyases / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D007218:Industrial Microbiology / Q000379:methods; D009875:Operator Regions, Genetic; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D012097:Repressor Proteins; D000446:Aldehyde-Lyases; C036027:L-fuculosephosphate aldolase