Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase.
Cross-linked enzyme crystals (CLECs) are a versatile form of biocatalyst that can also be used for biosensor application. Laccase from Trametes versicolor (E.C.1.10.3.2) was crystallized, cross-linked and lyophilized with beta-cyclodextrin. The CLEC laccase was found to be highly active towards phenols like 2-amino phenol, guaiacol, catechol, pyrogallol, catechin and ABTS (non-phenolic). The CLEC laccase was embedded in 30% polyvinylpropylidone (PVP) gel and mounted into an electrode to make the sensor. The biosensor was used to detect the phenols in 50-1000 micromol concentration level. Phenols with lower molecular weight such as 2-amino phenol, catechol and pyrogallol gave a short response time where as the higher molecular weight substrates like catechin and ABTS had comparatively a long response time. The optimum pH of the analyte was 5.5-6.0 when catechol was used as substrate. The CLEC laccase retained good activity for over 3 months.
Roy
J Jegan
JJ
Bioactive Polymer Engineering Section, Polymer Science Division, Regional Research Laboratory (CSIR), Trivandrum 695019, India.
Abraham
T Emilia
TE
Abhijith
K S
KS
Kumar
P V Sujith
PV
Thakur
M S
MS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D003432:Cross-Linking Reagents; D003460:Crystallization; D007700:Kinetics; D042845:Laccase / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D020072:Polyporales / Q000201:enzymology; D011145:Polyvinyls
D003432:Cross-Linking Reagents; D010636:Phenols; D011145:Polyvinyls; D042845:Laccase