D-Serine uptake by isolated retinas is consistent with ASCT-mediated transport.
Uptake of the neuromodulator D-serine by isolated larval tiger salamander (Ambystoma tigrinum) retinas was measured using capillary electrophoresis (CE). Excised retinas were incubated in Ringer's solution in the presence of 5 microM D-serine. The supernatant was removed after 30 min, mixed with 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F) to fluorescently label amines and analyzed using CE. Significant D-serine uptake was observed over a period of 1.5h. This is the first observation of D-serine uptake by an intact retinal tissue. D-Serine uptake in the retina was Na(+)-dependent and blocked by l-alanine, l-threonine, and l-cysteine. This pharmacology is consistent with the sodium dependent heteroexchange expected of system ASC-type transporters.
O'Brien
Kylie B
KB
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
Miller
Robert F
RF
Bowser
Michael T
MT
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000378:metabolism; D000409:Alanine / Q000494:pharmacology; D000557:Ambystoma; D026962:Amino Acid Transport System ASC / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D003545:Cysteine / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000379:methods; D066298:In Vitro Techniques; D012160:Retina / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D013912:Threonine / Q000494:pharmacology; D013997:Time Factors
D026962:Amino Acid Transport System ASC; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D013912:Threonine; D012694:Serine; D012964:Sodium; D009327:4-Chloro-7-nitrobenzofurazan; D003545:Cysteine; D000409:Alanine