Thermal injury and sepsis modulates beta-adrenergic receptors and cAMP responses in monocyte-committed bone marrow cells.
We have previously reported that adrenergic stimulation enhances monocytopoiesis following experimental burn injury and sepsis (BI/S). In the present work we measured beta-adrenergic receptor number and affinity in bone marrow committed monocyte progenitor cells (CD59(+)) following BI/S. We find that BI/S treatment significantly decreased monocyte progenitor cell beta-adrenergic receptors but significantly increased receptor binding affinity and isoproterenol-stimulated cAMP production. CD14 expression in macrophages derived in vitro from CD59(+) cells following BI/S was significantly increased by epinephrine and this change was blocked by beta(2)-adrenergic receptor antagonist. PCR analysis suggests the presence of beta(2)- but not beta(1)-adrenergic receptors. Enhanced adrenergic receptor signaling in CD59(+) bone marrow cells following BI/S may be important in macrophage development.
Muthu
Kuzhali
K
Department of Physiology, Loyola University Medical Center, 2160 South First Ave., Maywood, IL 60153, United States.
Deng
Jiangping
J
Romano
Fred
F
He
L-K
LK
Gamelli
Richard
R
Shankar
Ravi
R
Jones
Stephen B
SB
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D002056:Burns / Q000276:immunology* / Q000378:metabolism* / Q000401:mortality; D018957:CD59 Antigens / Q000096:biosynthesis; D002454:Cell Differentiation / Q000276:immunology; D000242:Cyclic AMP / Q000819:agonists / Q000096:biosynthesis*; D007424:Intracellular Fluid / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018805:Sepsis / Q000276:immunology* / Q000378:metabolism* / Q000401:mortality; D015854:Up-Regulation / Q000276:immunology
D018957:CD59 Antigens; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D000242:Cyclic AMP