Fabrication of a hydrogen peroxide biosensor based on a self-assemble composite oxide film.
A titania film with horseradish peroxidase (HRP) was immobilized on indium tin oxide (ITO) electrodes to act as a biosensor of hydrogen peroxide via the self-assembling and sol-gel route. The film was characterized by the atomic functional microscopy, electrochemical and UV-vis spectroscopy methods. The resulting biosensor exhibited fast amperometric response and good stability to hydrogen peroxide (H2O2). The linear range for H2O2 determination was from 2 x10(-6) to 1.45 x 10(-3) M, with a detection limit of 4 x10(-6) M based on S/N=3. The apparent Michaelis-Menten constant of the biosensor to H2O2 was estimated to be 1.5 mM, showing the HRP on the films had the higher biologic activities.
Fu
Ruiling
R
Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing, 210093, China.
Xu
Jinzhong
J
Li
Yu-Ru
YR
Wei
Yan-Li
YL
Zhu
Jun-Jie
JJ
D015374:Biosensing Techniques / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry*; D014466:Ultraviolet Rays
D010087:Oxides; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase