Highly fluorescent streptavidin-coated CdSe nanoparticles: preparation in water, characterization, and micropatterning.
CdSe quantum dots (QDs) with a high fluorescence quantum yield of 25% and a narrow size distribution were synthesized in a single step in water using glutathione as a stabilizing molecule. The exceptional optical properties enabled for the first time the detection of in-water-prepared single quantum dots at room temperature. For application as fluorescent bioanalytical probes, the QDs were coated with streptavidin. These QDs self-assemble with high contrast on micropatterned biotin while preserving their optical properties and their capability to bind in addition biotinylated molecules, a prerequisite for the development of novel supramolecular structures and bioassays.
Bäumle
Monika
M
Stamou
Dimitrios
D
Segura
Jean-Manuel
JM
Hovius
Ruud
R
Vogel
Horst
H
D001710:Biotin; D019187:Cadmium Compounds; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005978:Glutathione; D049329:Nanostructures; D045663:Quantum Dots; D018036:Selenium Compounds; D019809:Streptavidin; D014867:Water
D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018036:Selenium Compounds; D014867:Water; D001710:Biotin; D019809:Streptavidin; C058667:cadmium selenide; D005978:Glutathione