title: Measurement of small dense low-density lipoprotein particles.
abstract: Low density lipoprotein (LDL) particles are heterogeneous with respect to their size, density and lipid composition. Among LDL particles, the smaller and denser LDL particles [small dense (sd) LDL] are more atherogenic and the sd LDL phenotype is strongly associated with development of coronary heart disease. Here we will review various methods for measurement of sd LDL. Although ultracentrifugation, nuclear magnetic resonance (NMR) spectroscopy and gradient-gel electrophoresis (GGE) are usually employed for the measurement of sd LDL, such methods are either too laborious or expensive for general clinical use. We recently established a simple precipitation method for the quantification of sd LDL. This method is applicable to routine clinical use and allows the rapid measurement of a large number of samples.
authors.affiliation: Division of Diabetes and Metabolism, First Department of Internal Medicine, Showa University School of Medicine, 1-5-8 Hattanodai, Shinagawa-ku, Tokyo 142-8666, Japan. hirano@med.showa-u.ac.jp
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hirano
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Gen
authors.initials: G
authors.lastname: Yoshino
mesh_terms: D011232:Chemical Precipitation; D003324:Coronary Artery Disease / Q000097:blood; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000737:chemistry*; D014461:Ultracentrifugation
chemical_list: D008077:Lipoproteins, LDL; D008345:Manganese; D006493:Heparin