Accumulation, transformation and tissue distribution of domoic acid, the amnesic shellfish poisoning toxin, in the common cuttlefish, Sepia officinalis.
Domoic acid (DA) is a phycotoxin produced by some diatoms, mainly from the Pseudo-nitzschia genus, and has been detected throughout the marine food web. Although DA has been frequently found in cephalopod prey such as crustaceans and fish, little is known about DA accumulation in these molluscs. This study presents the first data showing relevant concentrations of DA detected in the common cuttlefish, Sepia officinalis, which is one of the most studied cephalopod species in the world. Domoic acid was consistently found throughout 2003 and 2004 in the digestive gland of cuttlefish reaching concentrations of 241.7 microg DA g(-1). The highest DA values were detected during spring and summer months, periods when Pseudo-nitzschia occur in the plankton. In fact, Pseudo-nitzschia blooms preceded the highest DA concentrations in cuttlefish. Evaluation of DA tissue distribution showed elevated DA concentrations in the digestive gland and branchial hearts. Further, DA isomers comprised a relevant percentage of the toxin profile, indicating degradation and biotransformation of the toxin in the branchial hearts. The common cuttlefish, like other cephalopod species, plays a central position in the food web and might be a new DA vector to top predators like marine mammals. Human intoxications are not expected since DA was only seldom detected in the mantle and even then in very low levels (max 0.7 microg DA g(-1)). However, in some countries whole juvenile animals are consumed (i.e. without evisceration) and in this case they might represent a risk to human health. This study contributes to understanding the occurrence of phycotoxins in cephalopods and reveals a new member of the marine food web able to accumulate DA.
Costa
Pedro R
PR
Departamento de Ambiente Aquático, Instituto Nacional de Investigação Agrária e das Pescas (IPIMAR), Avenida de Brasília, 1449-006 Lisboa, Portugal. prcosta@ioimar.pt
Rosa
Rui
R
Duarte-Silva
Alexandra
A
Brotas
Vanda
V
Sampayo
Maria Antónia M
MA
D000818:Animals; D001267:Atlantic Ocean; D002851:Chromatography, High Pressure Liquid; D017377:Diatoms / Q000737:chemistry*; D004064:Digestive System / Q000378:metabolism; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D008387:Marine Toxins / Q000493:pharmacokinetics*; D013058:Mass Spectrometry; D008974:Mollusca / Q000378:metabolism*; D012621:Seasons
D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid