[Prevalence of C77G polymorphism in exon 4 of the CD45 gene in the Spanish population].
OBJECTIVE
A mutation (C77G) in exon 4 of the CD45 gene is the most common cause of CD45 abnormal splicing in European populations, which has been associated with an increased susceptibility to human immunodeficiency virus infection. We aimed to analyze the C77G frequency in the Spanish population.


METHODS
517 healthy donors. CD45RA and RO expression was determined in circulating T lymphocytes by flow cytometry. CD45 exon 4 sequencing was carried out in individuals with an abnormal coexpression of CD45 isoforms.


RESULTS
6/517 individuals presented CD45RA persistence on memory T cells; all of them were heterozygous for C77G mutation. The resulting allelic frequency was 0,58% (95% confidence interval, 0.23-1.32).


CONCLUSIONS
C77G is present in the Spanish population. Further studies to elucidate its clinical significance are needed.
Gil
Juana
J
Servicio de Inmunología, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain. juana.gil@madrid.org
Ruiz-Tíscar
José Luis
JL
Rodríguez-Sainz
Carmen
C
Hernández
Ana
A
Santamaría
Beatriz
B
García-Sánchez
Félix
F
Fernández-Cruz
Eduardo
E
D005091:Exons / Q000235:genetics*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D015995:Prevalence; D013030:Spain / Q000453:epidemiology
D017493:Leukocyte Common Antigens