Study of real-time lectin-carbohydrate interactions on the surface of a quartz crystal microbalance.
A quartz crystal microbalance (QCM) biosensor system for lectin-carbohydrate interactions has been developed. Yeast mannan was immobilised on polystyrene-coated quartz crystals, and interactions tested with the lectin concanavalin A (Con A). The biosensor could be easily operated, where mannan immobilisation and all binding analyses were performed in real-time using a flow-through system. The apparent binding constant for yeast mannan to Con A was estimated to be 0.4 microM, well in accordance to reported literature values. In addition, the effective concentration values (EC50-values) for a series of mannose/mannoside ligands, acting as competitors to the mannan/Con A interaction, were determined to range from 0.18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol.
Pei
Zhichao
Z
KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden.
Anderson
Henrik
H
Aastrup
Teodor
T
Ramström
Olof
O
D015374:Biosensing Techniques / Q000295:instrumentation*; D032449:Canavalia / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism*; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008351:Mannans / Q000378:metabolism*; D011791:Quartz; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D008351:Mannans; D003208:Concanavalin A; D011791:Quartz