Smg1 nonsense mutations do not abolish nonsense-mediated mRNA decay in Drosophila melanogaster.
Smg1 is a key component of nonsense-mediated decay (NMD) in Caenorhabditis elegans and mammals. Here we report that two nonsense alleles of the ortholog of Smg1 do not affect NMD in Drosophila melanogaster.
Chen
Zhenzhong
Z
Centre for Environmental Stress and Adaptation Research, Department of Genetics, University of Melbourne, Melbourne, Victoria 3010, Australia.
Smith
Katherine R
KR
Batterham
Philip
P
Robin
Charles
C
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D018389:Codon, Nonsense; D003433:Crosses, Genetic; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005260:Female; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics
D018389:Codon, Nonsense; D029721:Drosophila Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; C510482:nonC protein, Drosophila