title: Optical and MRI multifunctional nanoprobe for targeting gliomas.
abstract: A multifunctional nanoprobe capable of targeting glioma cells, detectable by both magnetic resonance imaging and fluorescence microscopy, was developed. The nanoprobe was synthesized by coating iron oxide nanoparticles with covalently bound bifunctional poly(ethylene glycol) (PEG) polymer, which were subsequently functionalized with chlorotoxin and the near-infrared fluorescing molecule Cy5.5. Both MR imaging and fluorescence microscopy showed significant preferential uptake of the nanoparticle conjugates by glioma cells. Such a nanoprobe could potentially be used to image resections of glioma brain tumors in real time and to correlate preoperative diagnostic images with intraoperative pathology at cellular-level resolution.
authors.affiliation: Department of Materials Science & Engineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Omid
authors.initials: O
authors.lastname: Veiseh
authors.forename: Conroy
authors.initials: C
authors.lastname: Sun
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gunn
authors.forename: Nathan
authors.initials: N
authors.lastname: Kohler
authors.forename: Patrik
authors.initials: P
authors.lastname: Gabikian
authors.forename: Donghoon
authors.initials: D
authors.lastname: Lee
authors.forename: Narayan
authors.initials: N
authors.lastname: Bhattarai
authors.forename: Richard
authors.initials: R
authors.lastname: Ellenbogen
authors.forename: Raymond
authors.initials: R
authors.lastname: Sze
authors.forename: Andrew
authors.initials: A
authors.lastname: Hallahan
authors.forename: Jim
authors.initials: J
authors.lastname: Olson
authors.forename: Miqin
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry*; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D032383:Myocytes, Cardiac / Q000166:cytology; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D019047:Phantoms, Imaging; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D012604:Scorpion Venoms / Q000737:chemistry
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005290:Ferric Compounds; D012604:Scorpion Venoms; C091539:Chlorotoxin; C000499:ferric oxide; D011092:Polyethylene Glycols