title: Extraction of Mycobacterium tuberculosis DNA: a question of containment.
abstract: DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.
authors.affiliation: McGill University Health Centre Research Institute, Montreal, Quebec, Canada.
authors.forename: Wendy
authors.initials: W
authors.lastname: Somerville
authors.forename: Louise
authors.initials: L
authors.lastname: Thibert
authors.forename: Kevin
authors.initials: K
authors.lastname: Schwartzman
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Behr
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003264:Containment of Biohazards; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D019286:Endopeptidase K / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012150:Polymorphism, Restriction Fragment Length; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D002593:Cetrimonium Compounds; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012965:Sodium Chloride; D009113:Muramidase; D019286:Endopeptidase K; D000077286:Cetrimonium