title: A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging.
abstract: A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.
authors.affiliation: Central Laser Facility, Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 0QX, UK.
authors.forename: Stanley W
authors.initials: SW
authors.lastname: Botchway
authors.forename: Ignasi
authors.initials: I
authors.lastname: Barba
authors.forename: Randolf
authors.initials: R
authors.lastname: Jordan
authors.forename: Rebecca
authors.initials: R
authors.lastname: Harmston
authors.forename: Peter M
authors.initials: PM
authors.lastname: Haggie
authors.forename: Simon-Peter
authors.initials: SP
authors.lastname: Williams
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Fulton
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Parker
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
mesh_terms: D006916:5-Hydroxytryptophan / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D008856:Microscopy, Fluorescence; D010735:Phosphoglycerate Kinase / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006916:5-Hydroxytryptophan; D010735:Phosphoglycerate Kinase