[Gene frequencies and haplotypes of nine Y-short tandem repeat loci in four minority populations of China].
OBJECTIVE
To investigate the polymorphism of nine Y-short tandem repeat loci in 117 male individuals from four minority populations of China, and to obtain the polymorphism information in these populations.


METHODS
Nine loci in all samples were amplified by PCR. Products were electrophoresed on ABI PRISM 377 DNA sequencer. The electrophoresis result was analyzed by Genotyper 2.0 software. Allele frequencies, diversities and genetic distance were calculated.


RESULTS
Forty-three alleles and 32 haplotypes were found in Manchu population, 43 alleles and 33 haplotypes in Uygur population, 31 alleles and 15 haplotypes in Kirgiz population and 34 alleles and 23 haplotypes in Zhuang population. The closest genetic distance is 0.02530 between Manchu and Uygur, the highest genetic distance is 0.34590 between Kirgiz and Zhuang.


CONCLUSIONS
High haplotype diversities were found in four populations. The study of genetic diversity among different populations is useful in research of their origins, migrations and their relationships.
Yu
Liang
L
Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, Yunnan, 650118, PR China.
Huang
Xiao-qin
XQ
Shi
Li
L
Shi
Lei
L
Yu
Jian-kun
JK
Chu
Jia-you
JY
D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic