title: Phosphorylation of c-Jun in avian and mammalian motoneurons in vivo during programmed cell death: an early reversible event in the apoptotic cascade.
abstract: c-Jun is a transcription factor that is involved in various cellular events, including apoptotic cell death. For example, phosphorylation of c-Jun is one of the earliest biochemical changes detected in dying sympathetic neurons after NGF deprivation in vitro. However, currently, it is not known whether a similar molecular event is involved in the developmental programmed cell death (PCD) of neurons in vivo. We observed that only a subpopulation of motoneurons (MNs) exhibit c-Jun phosphorylation during the PCD period in chick [embryonic day 5 (E5)-E12] and mouse (E13-E18) embryos. Experimental perturbation of MN survival-promoting signals by limb bud removal (reduced signals) or by activity blockade (increased signals) in the chick embryo demonstrated that the presence of those signals is negatively correlated with the number of c-Jun-phosphorylated MNs. This suggests that insufficient survival signals (e.g., neurotrophic factors) may induce c-Jun phosphorylation of MNs in vivo. Consistent with the idea that c-Jun phosphorylation is a reversible event during normal PCD of MNs, we found that c-Jun phosphorylation was transiently observed in a subpopulation of mouse MNs rescued from PCD by deletion of the proapoptotic gene Bax. Inhibition of c-Jun signaling significantly reduced MN death in chick embryo, indicating that activation of c-Jun signaling is necessary for the PCD of MNs. Together, c-Jun phosphorylation appears to be required for the initiation of an early and reversible event in the intracellular PCD cascade in vivo after loss of survival-promoting signals such as neurotrophic factors.
authors.affiliation: Department of Anatomy, College of Medicine, Brain Korea 21, Korea University, Sungbuk-Gu, Seoul, Korea 136-705. woongsun@korea.ac.kr <woongsun@korea.ac.kr>
authors.forename: Woong
authors.initials: W
authors.lastname: Sun
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Gould
authors.forename: Jason
authors.initials: J
authors.lastname: Newbern
authors.forename: Carol
authors.initials: C
authors.lastname: Milligan
authors.forename: So Yoen
authors.initials: SY
authors.lastname: Choi
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Oppenheim
mesh_terms: D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D002642:Chick Embryo; D066298:In Vitro Techniques; D018878:Limb Buds / Q000196:embryology / Q000294:innervation; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009046:Motor Neurons / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D051028:bcl-2-Associated X Protein; D051057:Proto-Oncogene Proteins c-akt