Toxicity of organic compounds to marine invertebrate embryos and larvae: a comparison between the sea urchin embryogenesis bioassay and alternative test species.
This study investigated the toxic effects of the insecticides lindane and chlorpyrifos, the herbicide diuron, the organometallic antifoulant tributyltin (TBT), and the surfactant sodium dodecyl sulfate (SDS) on the early life stages of Paracentrotus lividus (Echinodermata, Euechinoidea), Ciona intestinalis (Chordata, Ascidiacea), Maja squinado and Palaemon serratus (Arthropoda, Crustacea) in laboratory acute toxicity tests. The assays studied embryogenesis success from fertilized egg to normal larvae in P. lividus (48 h incubation at 20 degrees C) and C. intestinalis (24 h incubation at 20 degrees C), and larval mortality at 24 and 48 h in M. squinado and P. serratus. For P. lividus, the median effective concentrations (EC50) reducing percentages of normal larvae by 50% were: 350 microg l(-1) for chlorpyrifos, 5500 microg l(-1) for diuron, 4277 microg l(-1) for SDS, and 0.309 microg l(-1) for TBT. For C. intestinalis, the EC50 values affecting embryogenesis success were 5666 microg l(-1) for chlorpyrifos, 24,397 microg (l-1) for diuron, 4412 microg l(-1) for lindane, 5145 microg I(-1) for SDS, and 7.1 microg l(-1) for TBT. The median lethal concentrations (LC50) for M. squinado larval survival were 0.84 microg l(-1) (24 h) and 0.79 microg l(-1) (48 h) for chlorpyrifos, 2.23 microg(l(-1) (24 h) and 2.18 microg l(-1) (48 h) for lindane, and 687 microg l(-1) (48 h) for SDS. For P. serratus the LC50 values obtained were 0.35 microg l(-1) (24 h) and 0.22 microg l(-1) (48 h) for chlorpyrifos, 3011 microg l(-1) (24 h) and 3044 microg l(-1) (48 h) for diuron, 5.20 microg l(-1) (24 h) and 5.59 microg l(-1) (48 h) for lindane, and 22.30 microg l(-1) (24 h) and 17.52 microg l(-1) (48 h) for TBT. Decapod larvae, as expected, were markedly more sensitive to the insecticides than sea urchins and ascidians, and SDS was the least toxic compound tested for these organisms. Lowest observed effect concentrations (LOEC) of TBT for sea urchin and ascidian embryos, chlorpyrifos and lindane for crustacean larvae, and SDS, were similar to those found in many coastal areas indicating that there would be a risk to invertebrate embryos and larvae from exposure in the field to these pollutants.
Bellas
Juan
J
Laboratorio de Ecoloxía Mariña, Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, Campus Lagoas-Marcosende, E-36200, Vigo, Galicia, Spain. juan.bellas@uvigo.es
Beiras
Ricardo
R
Mariño-Balsa
José Carlos
JC
Fernández
Nuria
N
D000818:Animals; D001681:Biological Assay; D004390:Chlorpyrifos / Q000592:standards / Q000633:toxicity; D004237:Diuron / Q000592:standards / Q000633:toxicity; D047108:Embryonic Development / Q000187:drug effects; D006540:Herbicides / Q000592:standards / Q000633:toxicity*; D001556:Hexachlorocyclohexane / Q000592:standards / Q000633:toxicity; D007306:Insecticides / Q000592:standards / Q000633:toxicity*; D007448:Invertebrates / Q000187:drug effects* / Q000254:growth & development; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D019055:No-Observed-Adverse-Effect Level; D018570:Risk Assessment; D012617:Sea Urchins; D012623:Seawater; D012967:Sodium Dodecyl Sulfate / Q000592:standards / Q000633:toxicity; D013501:Surface-Active Agents / Q000592:standards / Q000633:toxicity*; D014220:Trialkyltin Compounds / Q000592:standards / Q000633:toxicity
D006540:Herbicides; D007306:Insecticides; D013501:Surface-Active Agents; D014220:Trialkyltin Compounds; D012967:Sodium Dodecyl Sulfate; C011559:tributyltin; D001556:Hexachlorocyclohexane; D004237:Diuron; D004390:Chlorpyrifos