Quantitative detection of species-specific DNA in feedstuffs and fish meals.
A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species-derived material in concentrate feed mixtures and fish meals.
Krcmar
Pavel
P
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic. krcmar@vri.cz
Rencova
Eva
E
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000235:genetics; D002645:Chickens / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis*; D005396:Fish Products / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D017096:Prion Diseases / Q000517:prevention & control; D012418:Ruminants; D012680:Sensitivity and Specificity; D012756:Sheep / Q000235:genetics; D013045:Species Specificity; D013552:Swine / Q000235:genetics
D004272:DNA, Mitochondrial