RpoS-mediated growth-dependent expression of the Escherichia coli tkt genes encoding transketolases isoenzymes.
Escherichia coli tktA and tktB genes encode two transketolase isoenzymes involved in the pentose-phosphate pathway, In this study, two reporter lacZ fusions, tktA- and tktB-lacZ, were constructed to examine their transcriptional regulation on the E. coli chromosome. The tktA gene was induced in the exponential growth phase and suppressed in the stationary growth phase. However, the genetic elimination of the rpoS, whose product is an alternative sigma factor (RpoS), derepressed the tktA gene expression in the stationary growth phase, indicating that the RpoS sigma factor negatively regulates the tktA gene expression in the stationary growth phase. On the contrary, the tktB gene expression showed the highest value in the stationary growth phase and the RpoS positively regulated the tktB gene expression in the stationary growth phase. We also verified the role of the RpoS affecting the regulation of the tktA and tktB gene expression by the reverse transcription (RT)-PCR experiments. These results suggest that the differential growth-dependent expressions of the tktA and tktB genes are caused by the RpoS action.
Jung
Il Lae
IL
Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, 105, Yusong Taejon, 305-600, Korea.
Phyo
Ki Heon
KH
Kim
In Gyu
IG
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003470:Culture Media; D048168:Escherichia coli K12 / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014174:Transketolase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D007527:Isoenzymes; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D014174:Transketolase