title: Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination.
abstract: The gene encoding histamine dehydrogenase in Rhizobium sp. 4--9 has been cloned and overexpressed in Escherichia coli. The coding region of the gene was 2,079 bp and encoded a protein of 693 amino acids with a calculated molecular mass of 76,732 Da. This histamine dehydrogenase was related to histamine dehydrogenase from Nocardioides simplex (54.5% identical), trimethylamine dehydrogenase from Methylophilus methylotrophus (39.3% identical) and dimethylamine dehydrogenase from Hyphomicrobium X (38.1% identical), which have a covalent 6-S-cysteinyl flavin mononucleotide and a [4Fe--4S] cluster as redox cofactors. Sequence alignment and a UV-visible absorption spectrum supported the presence of these cofactors in this histamine dehydrogenase. The investigation of the enzymatic properties suggested that this enzyme exhibited the most excellent substrate specificity toward histamine among all amine oxidases or dehydrogenases found to date. The recombinant enzyme was able to be used for the colorimetric determination of histamine, which gave a linear calibration curve and identical data with conventional methods.
authors.affiliation: Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278-0037, Japan. mbakke@mail.kikkoman.co.jp
authors.forename: Mikio
authors.initials: M
authors.lastname: Bakke
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Sato
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ichikawa
authors.forename: Ikuko
authors.initials: I
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005502:Food; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity; D005517:Foodborne Diseases / Q000175:diagnosis*; D006632:Histamine / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009841:Oligonucleotides / Q000737:chemistry; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics; D010455:Peptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012231:Rhizobium / Q000201:enzymology* / Q000235:genetics; D013053:Spectrophotometry; D013379:Substrate Specificity; D013696:Temperature; D014413:Tuna; D014466:Ultraviolet Rays
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides; D010455:Peptides; D011994:Recombinant Proteins; D006632:Histamine; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; C020064:dimethylamine dehydrogenase; C506665:histamine dehydrogenase