[Construction, expression and bioactivity characterization of targeting toxin DT-VEGF].
Tumor rapid growth depends on neovascularization. Vascular endothelial growth factor, the main mediator during the occurrence and formation of vascularization, has specific receptors whose expression rate shows difference of orders of magnitude between tumor and the normal tissue, so it can be used to transport toxin molecules to the proliferative tumor endothelial and kill cancer cells. In our experiment, we constructed fusion protein DT-VEGF by linking diphtheria toxin's forward 389 amino acids's gene and VEGF165 via a linker. DT-VEGF is expressed in E. coli and purified. Our experiment proves in can kill vascular endothelial cells specifically, and the inhibition of neovascularization of chicken chorionic membrane is also confirmed.
Chai
Ying-Shuang
YS
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Cheng
Xin
X
Yao
Li-Hong
LH
Chen
Ai-Jun
AJ
Yu
Hong
H
Yan
Xin-Rui
XR
Jia
Run-Qing
RQ
Huang
Guo-Jin
GJ
Zhang
Zhi-Qing
ZQ
D020533:Angiogenesis Inhibitors / Q000096:biosynthesis; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D000922:Immunotoxins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics
D020533:Angiogenesis Inhibitors; D004167:Diphtheria Toxin; D000922:Immunotoxins; D011993:Recombinant Fusion Proteins; D042442:Vascular Endothelial Growth Factors