title: Asymmetrical distribution of ion channels in canine and human left-ventricular wall: epicardium versus midmyocardium.
abstract: The aim of the present study was to compare the distribution of ion currents and the major underlying ion channel proteins in canine and human subepicardial (EPI) and midmyocardial (MID) left-ventricular muscle. Ion currents and action potentials were recorded from canine cardiomyocytes derived from the very superficial EPI and central MID regions of the left ventricle. Amplitude, duration and the maximum velocity of depolarization of the action potential were significantly greater in MID than EPI myocytes, whereas phase-1 repolarization was more pronounced in the EPI cells. Amplitudes of the transient outwards K+ current (29.5+/-1.5 vs. 19.0+/-2.3 pA/pF at +50 mV) and the slow component of the delayed rectifier K+ current (10.3+/-2.3 vs. 6.5+/-1.0 pA/pF at +50 mV) were significantly larger in EPI than in MID myocytes under whole-cell voltage-clamp conditions. The densities of the inwards rectifier K+ current, rapid delayed rectifier K+ current and L-type Ca2+ current were similar in both cell types. Expression of channel proteins in both canine and human ventricular myocardium was determined by Western blotting. In the canine heart, the expression of Kv4.3, Kv1.4, KChIP2 and KvLQT1 was significantly higher, and that of Nav1.5 and MinK much lower, in EPI than in MID. No significant EPI-MID differences were observed in the expression of the other channel proteins studied (Kir2.1, alpha1C, HERG and MiRP1). Similar results were obtained in human hearts, although the HERG was more abundant in the EPI than in the MID layer. In the canine heart, the EPI-MID differences in ion current densities were proportional to differences in channel protein expression. Except for the density of HERG, the pattern of EPI-MID distribution of ion-channel proteins was identical in canine and human ventricles.
authors.affiliation: Department of Physiology, University of Debrecen, 4012 Debrecen, P.O. Box 22, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Szabó
authors.forename: Norbert
authors.initials: N
authors.lastname: Szentandrássy
authors.forename: Tamás
authors.initials: T
authors.lastname: Bíró
authors.forename: Balázs I
authors.initials: BI
authors.lastname: Tóth
authors.forename: Gabriella
authors.initials: G
authors.lastname: Czifra
authors.forename: János
authors.initials: J
authors.lastname: Magyar
authors.forename: Tamás
authors.initials: T
authors.lastname: Bányász
authors.forename: András
authors.initials: A
authors.lastname: Varró
authors.forename: László
authors.initials: L
authors.lastname: Kovács
authors.forename: Péter P
authors.initials: PP
authors.lastname: Nánási
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D020746:Calcium Channels, L-Type / Q000502:physiology; D004285:Dogs; D051638:Ether-A-Go-Go Potassium Channels / Q000032:analysis; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D032383:Myocytes, Cardiac / Q000502:physiology; D010496:Pericardium / Q000502:physiology*; D016276:Ventricular Function
chemical_list: D020746:Calcium Channels, L-Type; D051638:Ether-A-Go-Go Potassium Channels; D007473:Ion Channels; C092509:KCNH6 protein, human