Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli.
Polythioesters newly emerged as a type of novel polymer and they have showed great potential for application in industries. In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters. When 3-mercaptopropionate and 3-hydroxybutyrate were fed, poly (3-mercaptopropoinate) [poly (3MP)] and poly(3-mercaptopropionate-co-3-hydroxybutyrate) [poly(3MP-co-3HB)] were synthesized by recombinant Escherichia coli JM109 (pBPP1) harboring the constructed metabolic pathway. Results indicated clearly that all these genes are necessary for the synthesis of poly(3MP) and poly(3MP-co-3HB).
Liu
Shuang-Jiang
SJ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. shuangjiang@hotmail.com
Lütke-Eversloh
Tina
T
Steinbüchel
Alexander
A
D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D015097:3-Mercaptopropionic Acid / Q000737:chemistry*; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008954:Models, Biological; D008970:Molecular Weight; D010709:Phosphate Acetyltransferase / Q000235:genetics / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
D011108:Polymers; D015097:3-Mercaptopropionic Acid; D000217:Acyltransferases; C060970:poly-beta-hydroxybutyrate polymerase; D010709:Phosphate Acetyltransferase; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019285:butyrate kinase; D020155:3-Hydroxybutyric Acid