title: recA gene expression in a streptomycete is mediated by the unusual C-terminus of RecA protein.
abstract: Streptomyces RecA proteins are characterized by a conserved, positively charged extension of unknown function appended at their C-termini. To investigate the function of this element, we introduced the Streptomyces rimosus recA gene and its mutant form encoding the protein with a C-terminal deletion into S. rimosus. Both transcript and protein levels were dramatically increased in the strain expressing the truncated gene compared to the strain bearing the wild-type recA, indicating involvement of the characteristic C-terminal extension in regulating the recA expression in Streptomyces. Considering that RecA acts as a major regulator of DNA damage response in bacteria, this mode of regulation is expected to have broader implications and significance that outreaches our current understanding of RecA autoregulation.
authors.affiliation: Department of Molecular Biology, Rudjer Boskovic Institute, pp180, 10002 Zagreb, Croatia. iahel@irb.hr
authors.forename: Ivan
authors.initials: I
authors.lastname: Ahel
authors.forename: Andreja
authors.initials: A
authors.lastname: Mikoc
authors.forename: Vera
authors.initials: V
authors.lastname: Gamulin
mesh_terms: D000595:Amino Acid Sequence; D004249:DNA Damage; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D011938:Rec A Recombinases