Environmental tobacco smoke in rats.
OBJECTIVE
We conducted this study to identify the effect of subchronic cigarette smoke inhalation on the vocal cord mucosa of the rat.


METHODS
Experimental study.


METHODS
Department of Otolaryngology, School of Medicine, Karadeniz Technical University, Trabzon, Turkey


METHODS
Twenty-four male Wistar rats were divided randomly into experimental and control groups. The experimental group of rats was exposed to cigarette smoke for 2 hours each day over 60 consecutive days, and the control group was treated in an identical fashion but was exposed only to room air.


METHODS
Transmission electron microscopic examination of the rat vocal cords.


RESULTS
We found very high levels of plasma thiocyanate after exposure to smoke in the experimental group but no increase in the control group. In the experimental group, mean plasma thiocyanate concentrations of 3.7 +/- 0.5 and 119 +/- 11.4 mmol/L were measured before and after exposure to tobacco smoke, respectively (p < .05). On the contrary, mean plasma thiocyanate levels of control rats were found to be 3.0 +/- 0.4 and 3.9 +/- 0.4 mmol/L, respectively, before and after exposure. In the experimental group, the epithelium covering the true vocal cords shows disturbed stratification. Some cells exhibit loss of desmosomal connections between cells and enlargement of the intercellular space.


CONCLUSIONS
Our study demonstrates that the larynx in laboratory animals is one of the sensitive and useful targets for assessing the harmful effects associated with environmental tobacco smoke.
Işik
A Cemal Umit
AC
Department of Otolaryngology, School of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Kalender
Yusuf
Y
Yardimci
Serdar
S
Ergün
Ahmet
A
D000641:Ammonia / Q000032:analysis; D000818:Animals; D008297:Male; D046529:Microscopy, Electron, Transmission; D009538:Nicotine / Q000494:pharmacology; D009566:Nitrates / Q000032:analysis; D009585:Nitrogen Dioxide / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013458:Sulfur Dioxide / Q000032:analysis; D014028:Tobacco Smoke Pollution; D014827:Vocal Cords / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*
D009566:Nitrates; D014028:Tobacco Smoke Pollution; D013458:Sulfur Dioxide; D009538:Nicotine; D000641:Ammonia; D009585:Nitrogen Dioxide