Unravelling the structure of the pneumococcal autolytic lysozyme.
The LytC lysozyme of Streptococcus pneumoniae forms part of the autolytic system of this important pathogen. This enzyme is composed of a C-terminal CM (catalytic module), belonging to the GH25 family of glycosyl hydrolases, and an N-terminal CBM (choline-binding module), made of eleven homologous repeats, that specifically recognizes the choline residues that are present in pneumococcal teichoic and lipoteichoic acids. This arrangement inverts the general assembly pattern of the major pneumococcal autolysin, LytA, and the lytic enzymes encoded by pneumococcal bacteriophages that place the CBM (made of six repeats) at the C-terminus. In the present paper, a three-dimensional model of LytC built by homology modelling of each module and consistent with spectroscopic and hydrodynamic studies is shown. In addition, the putative catalytic-pair residues are identified. Despite the inversion in the modular arrangement, LytC and the bacteriophage-encoded Cpl-1 lysozyme most probably adopt a similar global fold. However, the distinct choline-binding ability and their substrate-binding surfaces may reflect a divergent evolution directed by the different roles played by them in the host (LytC) or in the bacteriophage (Cpl-1). The tight binding of LytC to the pneumococcal envelope, mediated by the acquisition of additional choline-binding repeats, could facilitate the regulation of the potentially suicidal activity of this autolysin. In contrast, a looser attachment of Cpl-1 to the cell wall and the establishment of more favourable interactions between its highly negatively charged catalytic surface and the positively charged chains of pneumococcal murein could enhance the lytic activity of the parasite-encoded enzyme and therefore liberation of the phage progeny.
Monterroso
Begoña
B
Instituto de Química-Física Rocasolano (CSIC), Serrano 119, 28006 Madrid, Spain.
López-Zumel
Consuelo
C
García
José L
JL
Sáiz
José L
JL
García
Pedro
P
Campillo
Nuria E
NE
Menéndez
Margarita
M
D000595:Amino Acid Sequence; D001433:Bacteriolysis; D001665:Binding Sites; D020134:Catalytic Domain; D002942:Circular Dichroism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry*; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017550:Spectroscopy, Fourier Transform Infrared; D013296:Streptococcus pneumoniae / Q000201:enzymology*
D009113:Muramidase; C419222:LytC protein, Streptococcus; D009238:N-Acetylmuramoyl-L-alanine Amidase