title: Genetic and environmental determinants of risk for cholangiocarcinoma via Opisthorchis viverrini in a densely infested area in Nakhon Phanom, northeast Thailand.
abstract: Infection with Opisthorchis viverrini (OV) is associated with cholangiocarcinoma. OV is common in northeast Thailand, but less than 10% of the inhabitants develop cholangiocarcinoma. Animal experiments suggest that OV infection alone does not cause cholangiocarcinoma, and thus other environmental and genetic factors may play a role in causation. We conducted a population-based case-control study in which sex, age and place of residence were matched individually. Polymorphisms of GSTM1 and GSTT1 alone were not associated with risk for cholangiocarcinoma, while an elevated level of antibodies against OV (ELISA) > or = 0.200 was the strongest risk indicator (odds ratio as compared to that <0.200 = 27.09 [95% confidence interval (CI): 6.30-116.57]. Compared to subjects who had a normal antibody range and the wild-type GSTM1 gene, those who had elevated antibodies had higher odds ratios of 10.34 (95% CI: 1.31-81.63) [corrected] for wild-type GSTM1 and 18.00 (95% CI: 3.33-97.40) [corrected] for the null variant thereof, respectively. Past and current regular drinkers of alcohol had higher risk [odds ratio = 5.39 (95% CI: 1.11-26.06) and 4.82 (95% CI: 1.29-18.06), respectively]. Eating fermented products was an independent risk factor. Smokers or consumers of fermented fish had substantially increased risk if they were past or current drinkers. Infection with OV correlates strongly with cholangiocarcinoma, susceptibility to which may be possibly associated with GSTM1 polymorphism. Alcohol may affect metabolic pathways of endogenous and exogenous nitrosamines.
authors.affiliation: Epidemiology Unit, Research Institute, Tochigi Cancer Centre, Utsunomiya, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Honjo
authors.forename: Petcharin
authors.initials: P
authors.lastname: Srivatanakul
authors.forename: Hutcha
authors.initials: H
authors.lastname: Sriplung
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kikukawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Hanai
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Uchida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Todoroki
authors.forename: Adisorn
authors.initials: A
authors.lastname: Jedpiyawongse
authors.forename: Pacharin
authors.initials: P
authors.lastname: Kittiwatanachot
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
authors.forename: Somyos
authors.initials: S
authors.lastname: Deerasamee
authors.forename: Masanao
authors.initials: M
authors.lastname: Miwa
mesh_terms: D000818:Animals; D001483:Base Sequence; D018281:Cholangiocarcinoma / Q000453:epidemiology* / Q000469:parasitology; D017931:DNA Primers; D006801:Humans; D008019:Life Style; D009889:Opisthorchiasis / Q000150:complications* / Q000453:epidemiology; D009891:Opisthorchis / Q000302:isolation & purification*; D012307:Risk Factors; D013785:Thailand / Q000453:epidemiology
chemical_list: D017931:DNA Primers