title: [Expression of phytase gene in Bombyx mori].
abstract: Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
authors.affiliation: Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang 212018, China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Xiao
authors.forename: Ping
authors.initials: P
authors.lastname: Ji
authors.forename: Sheng-Peng
authors.initials: SP
authors.lastname: Wang
authors.forename: Jia-Lu
authors.initials: JL
authors.lastname: He
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006863:Hydrogen-Ion Concentration
chemical_list: D005656:Fungal Proteins; D010832:6-Phytase