title: Biochemical and molecular characterization of a tetrachloroethene dechlorinating Desulfitobacterium sp. strain Y51: a review.
abstract: A strict anaerobic bacterium, Desulfitobacterium sp. strain Y51, is capable of very efficiently dechlorinating tetrachloroethene (PCE) via trichloroethene (TCE) to cis-1,2-dichloroethene (cis-DCE) at concentrations as high as 960 microM and as low as 0.06 microM. Dechlorination was highly susceptible to air oxidation and to potential alternative electron acceptors, such as nitrite, nitrate or sulfite. The PCE reductive dehalogenase (encoded by the pceA gene and abbreviated as PceA dehalogenase) of strain Y51 was purified and characterized. The purified enzyme catalyzed the reductive dechlorination of PCE to cis-DCE at a specific activity of 113.6 nmol min(-1) mg protein(-1). The apparent K(m) values for PCE and TCE were 105.7 and 535.3 microM, respectively. In addition to PCE and TCE, the enzyme exhibited dechlorination activity for various chlorinated ethanes such as hexachloroethane, pentachloroethane, 1,1,1,2-tetrachloroethane and 1,1,2,2-tetrachloroethane. An 8.4-kb DNA fragment cloned from the Y51 genome revealed eight open reading frames, including the pceAB genes. Immunoblot analysis revealed that PceA dehalogenase is localized in the periplasm of Y51 cells. Production of PceA dehalogenase was induced upon addition of TCE. Significant growth inhibition of strain Y51 was observed in the presence of cis-DCE, More interestingly, the pce gene cluster was deleted with high frequency when the cells were grown with cis-DCE.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan. kfurukaw@agr.kyushu-u.ac.jp
authors.forename: Kensuke
authors.initials: K
authors.lastname: Furukawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Suyama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Taiki
authors.initials: T
authors.lastname: Futagami
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Goto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001673:Biodegradation, Environmental; D045858:Desulfitobacterium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013750:Tetrachloroethylene / Q000378:metabolism*
chemical_list: D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase; D013750:Tetrachloroethylene