A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge.
We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal anti-enterovirus strategy.
Ahn
Jeonghyun
J
Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea.
Jun
Eun Seok
ES
Lee
Hui Sun
HS
Yoon
Seung Yong
SY
Kim
DongHou
D
Joo
Chul-Hyun
CH
Kim
Yoo Kyum
YK
Lee
Heuiran
H
D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D004770:Enterovirus / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000998:Antiviral Agents; D034741:RNA, Small Interfering; D014764:Viral Proteins; C097017:coxsackie B3 virus protein 2B