Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4.
Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-D forms. We show that the all-D variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 microM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.
Thevissen
Karin
K
Centre for Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium. karin.thevissen@agr.kuleuven.ac.be
François
Isabelle E J A
IE
Sijtsma
Lolke
L
van Amerongen
Aart
A
Schaaper
Wim M M
WM
Meloen
Rob
R
Posthuma-Trumpie
Truus
T
Broekaert
Willem F
WF
Cammue
Bruno P A
BP
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D001120:Arginine / Q000235:genetics; D005658:Fungi / Q000187:drug effects; D006461:Hemolysis; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D014364:Tryptophan / Q000235:genetics
C488078:AMP1 protein, Impatiens balsamina; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; C504508:Ib-AMP4 peptide, Impatiens balsamina; D010455:Peptides; D010456:Peptides, Cyclic; D010940:Plant Proteins; D014364:Tryptophan; D001120:Arginine