[Targeted adhesion of platelet receptor-specific microbubble agent to thrombus in vitro].
OBJECTIVE
To examine the thrombus-targeting effect of platelet receptor-specific lipid microbubbles.


METHODS
The targeted microbubbles were prepared by coupling Arg-Gly-Asp-Ser (RGDS) with the lipid microbubbles, which were added to the microthrombus generated by platelet aggregation. The effects of the targeted microbubbles on the ultrasonic signal was observed in an artificial thrombus model.


RESULTS
The targeted microbubbles were adhesive to the microthrombus, while the non-targeted microbubbles did not possess this property. The ultrasonic signal of the thrombus border was enhanced significantly after the addition of the targeted microbubbles.


CONCLUSIONS
Platelet receptor-specific microbubbles possess significant adhesive property to the thrombus and can improve signal-to-noise ratio of the thrombus, suggesting the potential value of the targeted microbubbles for clinical application in the diagnosis and treatment of thrombus.
Ou
Wen-chao
WC
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Xiu
Jian-cheng
JC
Xie
Jin-guo
JG
Zha
Dao-gang
DG
Liu
Jian
J
Hao
Rui
R
Bin
Jian-ping
JP
Liu
Yi-Li
YL
D002448:Cell Adhesion; D016503:Drug Delivery Systems; D006801:Humans; D045423:Microbubbles; D009842:Oligopeptides; D010974:Platelet Aggregation / Q000187:drug effects*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014463:Ultrasonography
D009842:Oligopeptides; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C046617:arginyl-glycyl-aspartyl-serine