Dose-dependent inhibition of gynecophoral canal protein gene expression in vitro in the schistosome (Schistosoma japonicum) by RNA interference.
The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose-dependent. RT-PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied.
Cheng
Guo-Feng
GF
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China.
Lin
Jiao-Jiao
JJ
Shi
Yi
Y
Jin
You-Xin
YX
Fu
Zhi-Qiang
ZQ
Jin
Ya-Mei
YM
Zhou
Yuan-Cong
YC
Cai
You-Min
YM
D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis*; D015801:Helminth Proteins / Q000096:biosynthesis*; D008297:Male; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal
D006023:Glycoproteins; D015801:Helminth Proteins; D012330:RNA, Double-Stranded; C103128:gynecophoral canal protein, 86-kDa