[Cloning tumor-related genes and tumor suppressor genes in glioma with polymerase chain reaction-based subtractive hybridization].
OBJECTIVE
Glioma is a common tumor in central nervous system with no specific clinical therapy. Its pathogenesis is unclear. This study was to clone tumor-related genes and tumor suppressor genes in glioma with polymerase chain reaction (PCR)-based subtractive hybridization, and to explore the molecular biological mechanism of tumorigenesis of glioma.


METHODS
mRNA was isolated from a sample of human glioma, and reversely transcribed into cDNA. PCR-based subtractive hybridization was used to clone tumor-related genes and tumor suppressor genes from it.


RESULTS
In tumor-related candidate gene group, phospho-protein enriched in astrocytes of 15 (PEA15) and homology of acid fibroblast growth factor (aFGF) were picked up. Whereas, in tumor suppressor gene group, interferon-induced protein 17 and ndr2 were picked up. ndr2 was widely expressed in normal brain tissue, but absent in glioma tissue.


CONCLUSIONS
ndr2 gene is a candidate tumor suppressor gene, and may play a role in tumorigenesis of glioma.
Deng
Yan-Chun
YC
Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P. R. China.
Wang
Ji-Cun
JC
Liu
Xin-Ping
XP
Ji
Shao-Ping
SP
Yao
Li-Bo
LB
D051017:Apoptosis Regulatory Proteins; D001932:Brain Neoplasms / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D016207:Cytokines / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics; D016220:Fibroblast Growth Factor 1 / Q000032:analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis; D009693:Nucleic Acid Hybridization / Q000379:methods; D009857:Oncogenes; D010750:Phosphoproteins / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics; D014452:Ubiquitins / Q000032:analysis
D051017:Apoptosis Regulatory Proteins; D016207:Cytokines; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C092039:PEA15 protein, human; D010750:Phosphoproteins; D012333:RNA, Messenger; D014452:Ubiquitins; D016220:Fibroblast Growth Factor 1; C074034:ISG15 protein, human; D017346:Protein Serine-Threonine Kinases; C496014:STK38L protein, human