title: Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells.
abstract: BACKGROUND
  Changes in the expression of galactose (Gal) alpha1-3Gal, swine lymphocyte antigen (SLA) class II and intracellular adhesion molecule (ICAM)-1 of single porcine pancreatic endocrine (PE) cells during the culture period were investigated.
  
  
  METHODS
  Cultured porcine PE-cells were fixed in 10% buffered formalin for histological evaluation. At 1, 3, 6, 9 and 12 weeks of culture, mRNA was obtained from porcine PE-cells so that the expression of SLA class II and ICAM-1 genes could be examined by reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  The rates of Galalpha1-3Gal and SLA class II-positive cells did not decrease during the culture period, but the rates of Galalpha1-3Gal and SLA class II strongly positive cells significantly decreased. ICAM-1-positive cells were scarcely observed during the culture period. SLA class II and ICAM-1 mRNAs were detected at 1 and 3 weeks of culture, but were not detected after 6 weeks of culture.
  
  
  CONCLUSIONS
  These results suggest that partial reduction in the expression of these antigens could be obtained by a long-term culture.
authors.affiliation: Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. edamura@brs.nihon-u.ac.jp
authors.forename: Kazuya
authors.initials: K
authors.lastname: Edamura
authors.forename: Koko
authors.initials: K
authors.lastname: Nasu
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Iwami
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Hisako
authors.initials: H
authors.lastname: Ohgawara
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D046508:Culture Techniques; D004703:Endocrine System / Q000166:cytology*; D005786:Gene Expression Regulation; D010179:Pancreas / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013552:Swine; D013997:Time Factors
chemical_list: D000941:Antigens; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger