title: beta-Microseminoprotein binds CRISP-3 in human seminal plasma.
abstract: beta-Microseminoprotein (MSP) and cysteine-rich secretory protein 3 (CRISP-3) are abundant constituents of human seminal plasma. Immunoprecipitation and gel filtration of seminal plasma proteins combined with examination of the proteins in their pure form showed that MSP and CRISP-3 form stable, non-covalent complexes. CRISP-3 binds MSP with very high affinity, as evidenced by surface plasmon resonance. Due to far higher abundance of MSP in prostatic fluid, it manifests large overcapacity for CRISP-3 binding. Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its aminoterminal SCP-domain.
authors.affiliation: Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Denmark.
authors.forename: Lene
authors.initials: L
authors.lastname: Udby
authors.forename: Ake
authors.initials: A
authors.lastname: Lundwall
authors.forename: Anders H
authors.initials: AH
authors.lastname: Johnsen
authors.forename: Per
authors.initials: P
authors.lastname: Fernlund
authors.forename: Camilla
authors.initials: C
authors.lastname: Valtonen-André
authors.forename: Anna M
authors.initials: AM
authors.lastname: Blom
authors.forename: Hans
authors.initials: H
authors.lastname: Lilja
authors.forename: Niels
authors.initials: N
authors.lastname: Borregaard
authors.forename: Lars
authors.initials: L
authors.lastname: Kjeldsen
authors.forename: Anders
authors.initials: A
authors.lastname: Bjartell
mesh_terms: D006801:Humans; D007700:Kinetics; D008297:Male; D029584:Prostatic Secretory Proteins / Q000378:metabolism*; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*
chemical_list: C489926:CRISP3 protein, human; D029584:Prostatic Secretory Proteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein