HCN subunit-specific and cAMP-modulated effects of anesthetics on neuronal pacemaker currents.
General anesthetics have been a mainstay of surgical practice for more than 150 years, but the mechanisms by which they mediate their important clinical actions remain unclear. Ion channels represent important anesthetic targets, and, although GABA(A) receptors have emerged as major contributors to sedative, immobilizing, and hypnotic effects of intravenous anesthetics, a role for those receptors is less certain in the case of inhalational anesthetics. The neuronal hyperpolarization-activated pacemaker current (Ih) is essential for oscillatory and integrative properties in numerous cell types. Here, we show that clinically relevant concentrations of inhalational anesthetics modulate neuronal Ih and the corresponding HCN channels in a subunit-specific and cAMP-dependent manner. Anesthetic inhibition of Ih involves a hyperpolarizing shift in voltage dependence of activation and a decrease in maximal current amplitude; these effects can be ascribed to HCN1 and HCN2 subunits, respectively, and both actions are recapitulated in heteromeric HCN1-HCN2 channels. Mutagenesis and simulations suggest that apparently distinct actions of anesthetics on V(1/2) and amplitude represent different manifestations of a single underlying mechanism (i.e., stabilization of channel closed state), with the predominant action determined by basal inhibition imposed by individual subunit C-terminal domains and relieved by cAMP. These data reveal a molecular basis for multiple actions of anesthetics on neuronal HCN channels, highlight the importance of proximal C terminus in modulation of HCN channel gating by diverse agents, and advance neuronal pacemaker channels as potentially relevant targets for clinical actions of inhaled anesthetics.
Chen
Xiangdong
X
Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA.
Sirois
Jay E
JE
Lei
Qiubo
Q
Talley
Edmund M
EM
Lynch
Carl
C
Bayliss
Douglas A
DA
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D001933:Brain Stem / Q000502:physiology; D054815:Cyclic Nucleotide-Gated Cation Channels; D017931:DNA Primers; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels; D051381:Rats; D015183:Restriction Mapping; D013788:Thalamus / Q000502:physiology
D000777:Anesthetics; D054815:Cyclic Nucleotide-Gated Cation Channels; D017931:DNA Primers; C578667:Hcn1 protein, rat; C578712:Hcn2 protein, rat; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D015221:Potassium Channels