Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin.
The presence of human Giardia in several animals suggests a zoonotic transmission. We studied G. Intestinalis isolates obtained from: children with diarrhea (n=6), asymptomatic children (n=7), axenic cultures (n=7) and dogs (n=11). The sequence corresponding to 16 S rRNA was amplified by PCR, sequenced and compared with genotypes A, B and Dog sequences reported in the Gene Bank database. Results show that 9/20 (45%) of children isolates belonged to genotype A and 11/20 (55%) showed some variable sites, allowing classification in three arbitrary clusters: A1, A2 and A3. In addition 7/11 (63%) of dog isolates were genotype A, including those dogs that lived in the same locality as the children lived, while 4/11 (37%) belonged to an arbitrary A4 cluster living in a different locality. In this study, genotype A was associated with samples from children and dogs, and, therefore, we could infer zoonotic transmission as a way of getting the disease.
Eligio-García
Leticia
L
Laboratory of Parasitology Research, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, 06720 DF, Mexico.
Cortes-Campos
Adrian
A
Jiménez-Cardoso
Enedina
E
D000818:Animals; D001483:Base Sequence; D002648:Child; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D020459:Genes, rRNA / Q000235:genetics; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology* / Q000635:transmission / Q000662:veterinary; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015047:Zoonoses
D016054:DNA, Protozoan; D004275:DNA, Ribosomal