IL-5 inhibits apoptosis by upregulation of c-myc expression in human hematopoietic cells.
Interleukin 5 (IL-5) inhibition of apoptosis is required throughout many hematopoietic lineages to regulate proliferation and differentiation. It is not clear how IL-5 mediates the intracellular molecular events regulating the anti-apoptotic effect. The cell lines TF-1 and JYTF-1 expressed different amounts of the IL-5 receptor alpha (IL-5R alpha) subunit, which caused contrasting effects in response to IL-5. IL-5 supported the survival but not the anti-apoptotic activities of TF-1 cells, which have a low expression of IL-5R alpha. In contrast, IL-5 supported both the survival and the anti-apoptotic activities of JYTF-1 cells, which overexpressed IL-5R alpha compared to TF-1 cells. In this study, IL-5 stimulation increased Annexin V binding (indicative of apoptosis) in TF-1 cells and decreased apoptosis in JYTF-1 cells. The proto-oncogenes c-fos, fosB, and c-jun were not detected, whereas junB was induced by IL-5 stimulation in both types of cells. Most importantly, IL-5 significantly induced c-myc expression in JYTF-1 cells, but did not in TF-1 cells. These results are consistent with the possibility that IL-5 inhibits apoptosis in JYTF-1 cells via the upregulation of c-myc expression.
Juan
Shu-Hui
SH
Graduate Institute of Medical Sciences, Taipei Medical University, Hsinyi District, Taipei 110, Taiwan.
Yen
Jeffrey Jong-Young
JJ
Lee
Horng-Mo
HM
Huang
Huei-Mei
HM
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D006413:Hematopoietic System / Q000378:metabolism*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics*
D015848:Interleukin-5; D016271:Proto-Oncogene Proteins c-myc