beta-Microseminoprotein binds CRISP-3 in human seminal plasma.
beta-Microseminoprotein (MSP) and cysteine-rich secretory protein 3 (CRISP-3) are abundant constituents of human seminal plasma. Immunoprecipitation and gel filtration of seminal plasma proteins combined with examination of the proteins in their pure form showed that MSP and CRISP-3 form stable, non-covalent complexes. CRISP-3 binds MSP with very high affinity, as evidenced by surface plasmon resonance. Due to far higher abundance of MSP in prostatic fluid, it manifests large overcapacity for CRISP-3 binding. Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its aminoterminal SCP-domain.
Udby
Lene
L
Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Denmark.
Lundwall
Ake
A
Johnsen
Anders H
AH
Fernlund
Per
P
Valtonen-André
Camilla
C
Blom
Anna M
AM
Lilja
Hans
H
Borregaard
Niels
N
Kjeldsen
Lars
L
Bjartell
Anders
A
D006801:Humans; D007700:Kinetics; D008297:Male; D029584:Prostatic Secretory Proteins / Q000378:metabolism*; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*
C489926:CRISP3 protein, human; D029584:Prostatic Secretory Proteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein