Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
alphabeta-Tubulin heterodimers, from which the microtubules of the cytoskeleton are built, have a complex chaperone-dependent folding pathway. They are thought to be unique to eukaryotes, whereas the homologue FtsZ can be found in bacteria. The exceptions are BtubA and BtubB from Prosthecobacter, which have higher sequence homology to eukaryotic tubulin than to FtsZ. Here we show that some of their properties are different from tubulin, such as weak dimerization and chaperone-independent folding. However, their structure is strikingly similar to tubulin including surface loops, and BtubA/B form tubulin-like protofilaments. Presumably, BtubA/B were transferred from a eukaryotic cell by horizontal gene transfer because their high degree of similarity to eukaryotic genes is unique within the Prosthecobacter genome.
Schlieper
Daniel
D
Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom.
Oliva
María A
MA
Andreu
José M
JM
Löwe
Jan
J
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002693:Chlamydiaceae / Q000378:metabolism; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006160:Guanosine Triphosphate / Q000737:chemistry; D008027:Light; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry; D008958:Models, Molecular; D018832:Molecular Chaperones; D009711:Nucleotides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012542:Scattering, Radiation; D014404:Tubulin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D018832:Molecular Chaperones; D009711:Nucleotides; D011108:Polymers; D014404:Tubulin; D006160:Guanosine Triphosphate