A capillary electrophoresis chip with hydrodynamic sample injection for measurements from a continuous sample flow.
A microchip-based capillary electrophoresis device supported by a microfluidic network made of poly(dimethylsiloxane), used for measuring target analytes from a continuous sample flow, is presented. The microsystem was fabricated by means of replica molding in combination with standard microfabrication technologies, resulting in microfluidic components and an electrochemical detector. A new hydrodynamic sample injection procedure is introduced, and the maximum number of consecutive measurements that can be made with a poly(dimethylsiloxane) capillary electrophoresis chip with amperometric detection is investigated with respect to reproducibility. The device features a high degree of functional integration, so the benefits associated with miniaturized analysis systems apply to it.
Büttgenbach
S
S
Technical University of Braunschweig, Institute for Microtechnology, Langer Kamp 8, 38106 Braunschweig, Germany. s.buettgenbach@tu-bs.de
Wilke
R
R
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D012828:Silicones / Q000737:chemistry*; D018511:Systems Integration
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon