title: Protective effect of melatonin against oxidative stress on adhesion formation in the rat cecum and uterine horn model.
abstract: This experimental study was designed to evaluate the degree of adhesion formation and peritoneal tissue levels of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) and the effect of melatonin on these metabolites in a postoperative intraperitoneal adhesion formation model in rats. Thirty adult female Wistar albino rats were subjected to standardized lesions by cecal and uterine horn abrasion and were randomly divided into three groups. Control rats were treated with 5% ethanol. Melatonin treated rats received 4 mg/kg melatonin before closure and for 10 consecutive days intraperitoneally after surgery. Rats in the sham operation group underwent a surgical procedure similar to the other groups however the peritoneal abrasion was not performed. On postoperative day 10 relaparatomy was performed. After the assessment of the adhesions, the rats in each group were sacrificed and peritoneal tissues were harvested to determine the tissue levels of MDA, GSH and NO activity. Adhesion formation scores in the melatonin group were significantly lower than that of control and sham group (p<0.01 and p<0.02, respectively). Tissue levels of MDA and NO were significantly lower in the melatonin treated rats when compared with control and sham groups. The levels of GSH in the melatonin treated rats were significantly higher than those of control and sham groups (p<0.01). The results demonstrate that in this experimental model, intraperitoneal administration of melatonin decreases the extent of peritoneal adhesions and causes a decrease in MDA and NO and an increase in GSH levels.
authors.affiliation: Department of General Surgery, Inonu University Medical Faculty, Malatya, Turkey. cara@inonu.edu.tr
authors.forename: Cengiz
authors.initials: C
authors.lastname: Ara
authors.forename: Hale
authors.initials: H
authors.lastname: Kirimlioglu
authors.forename: Aysun Bay
authors.initials: AB
authors.lastname: Karabulut
authors.forename: Sacit
authors.initials: S
authors.lastname: Coban
authors.forename: Seyma
authors.initials: S
authors.lastname: Hascalik
authors.forename: Onder
authors.initials: O
authors.lastname: Celik
authors.forename: Sezai
authors.initials: S
authors.lastname: Yilmaz
authors.forename: Vedat
authors.initials: V
authors.lastname: Kirimlioglu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002432:Cecum / Q000473:pathology*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control*; D014599:Uterus / Q000473:pathology*
chemical_list: D000975:Antioxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D005978:Glutathione; D008550:Melatonin