title: [Effect of epigallocatechingallate on acute lung injury induced by oleic acid in mice].
abstract: OBJECTIVE
  To investigate the effect of epigallocatechingallate (EGCG) on acute lung injury induced by oleic acid in mice and the possible mechanism.
  
  
  METHODS
  Acute lung injury was induced by oleic acid in mice. Light microscopy and electron microscopy were used to examine histological changes and lung index as well as wet to dry weight ratio was calculated. Serum TNF-a level was measured by enzyme linked immunosorbent assay (ELISA) and the phosphorylation of p38 MAPK was determined by Western blotting.
  
  
  RESULTS
  Pretreatment of EGCG significantly alleviated oleic acid induced lung injury accompanied by reduction of lung index and wet to dry weight ratio, decreased of TNF-a level in serum and inhibition of phosphorylation of p38 MAPK.
  
  
  CONCLUSIONS
  EGCG showed beneficial effect on acute lung injury induced by oleic acid in mice. The ultimate reduction of TNF-alpha in serum caused by inhibition of phosphorylated p38 MAPK is involved in the mechanism of action of EGCG.
authors.affiliation: Center for Drug Research and Development, College of Life Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Guang-lin
authors.initials: GL
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Yao
authors.forename: Shu-qin
authors.initials: SQ
authors.lastname: Yu
authors.forename: Dan
authors.initials: D
authors.lastname: Bu
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Zhu-nan
authors.initials: ZN
authors.lastname: Gong
authors.forename: Shuang-quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008168:Lung / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D019301:Oleic Acid; D010766:Phosphorylation / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D019301:Oleic Acid; D002392:Catechin; C045651:epigallocatechin gallate; D048051:p38 Mitogen-Activated Protein Kinases