title: [Ultrastructure and crystallin mutant molecular modeling of hereditary coralliform cataract].
abstract: To observe the correlation of gammaD-crystallin P23T mutant with lens ultrastructure of the hereditary coralliform cataract.
  
  
  
  Complete ophthalmologic examinations were performed before lens extraction and lens samples were studied by transmission and scanning electric microscope respectively. Protein molecular modeling was performed using SWISS-MODEL(version 2.0).
  
  
  
  Protein structure modeling demonstrated that the mutant caused a decrease in molecular final total energy and changes in the surface structure of gammaD-crystallin. Ultrastructure study revealed crystals deposited in lens, extensive granules dispersed in uncommon oval structure and the disorganization of lens epithelial cells.
  
  
  
  It is possible that the gammaD-crystallin P23T mutant is associated with abnormal crystals in lens and disorganization of lens epithelial cells.
authors.affiliation: The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Wei-zhen
authors.initials: WZ
authors.lastname: Xu
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.forename: Qi
authors.initials: Q
authors.lastname: Dong
authors.forename: Shan-rong
authors.initials: SR
authors.lastname: Cai
authors.forename: Ke
authors.initials: K
authors.lastname: Yao
authors.forename: Su-zhan
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D002386:Cataract / Q000151:congenital / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000648:ultrastructure*; D008297:Male; D010375:Pedigree; D010641:Phenotype; D017354:Point Mutation; D038222:gamma-Crystallins / Q000235:genetics*
chemical_list: D038222:gamma-Crystallins