[Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim].
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
Jin
Zhi-Ping
ZP
Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Zhao
De-Xiu
DX
Qiao
Chuan-Ling
CL
Qu
Wen-Quan
WQ
Chen
Ya-Qiong
YQ
Fu
Chun-Xiang
CX
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D030441:Saussurea / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D014157:Transcription Factors