title: [Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo].
abstract: OBJECTIVE
  To investigate the effect of IL-24 expression on the growth of glioma cells.
  
  
  METHODS
  The IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor.
  
  
  RESULTS
  It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.
  
  
  CONCLUSIONS
  IL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.
authors.affiliation: Cell Biology Division, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Li
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Shan
authors.forename: Yun-li
authors.initials: YL
authors.lastname: Yan
authors.forename: Li-fen
authors.initials: LF
authors.lastname: Zheng
authors.forename: Qiao-xia
authors.initials: QX
authors.lastname: Li
authors.forename: Na-jing
authors.initials: NJ
authors.lastname: Zhou
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D011994:Recombinant Proteins; D000099180:Interleukin-24