Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis.
A method has been developed for the separation and determination of phospholipids by nonaqueous capillary electrophoresis in a separation medium of acetonitrile-2-proponol (3:2, v/v), 0.3% acetic acid and 60 mM ammonium acetate. To optimize the separation conditions, the composition of separation medium including alcohols, acetic acid, n-hexane and ammonium acetate was studied. The solvation interaction and ion-dipole interaction were also investigated. The contents of phospholipids in soybean, sunflower, peanut, apricot kernel, filbert and walnut were determined by the recommended method. The results obtained by the nonaqueous capillary electrophoreses were in good agreement with those determined by micellar electrokinetic chromatography.
Guo
Bao-Yuan
BY
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Wen
Bei
B
Shan
Xiao-Quan
XQ
Zhang
Shu-Zheng
SZ
Lin
Jin-Ming
JM
D000085:Acetates; D019342:Acetic Acid; D000438:Alcohols; D020374:Chromatography, Micellar Electrokinetic Capillary; D019075:Electrophoresis, Capillary / Q000379:methods*; D006586:Hexanes; D006863:Hydrogen-Ion Concentration; D010743:Phospholipids / Q000032:analysis / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
D000085:Acetates; D000438:Alcohols; D006586:Hexanes; D010743:Phospholipids; C026385:n-hexane; D019342:Acetic Acid; C018824:ammonium acetate