RNA polymerase structure and function at lac operon.
Transcription of E. coli lac operon by RNA polymerase (RNAP) is a classic example of how the basic functions of this enzyme, specifically the ability to recognize/bind promoters, melt the DNA and initiate RNA synthesis, is positively regulated by transcription activators, such as cyclic AMP-receptor protein, CRP, and negatively regulated by lac-repressor, LacI. In this review, we discuss the recent progress in structural and biochemical studies of RNAP and its binary and ternary complexes with CRP and lac promoter. With structural information now available for RNAP and models of binary and ternary elongation complexes, the interaction between these factors and RNAP can be modeled, and possible molecular mechanisms of their action can be inferred.
Borukhov
Sergei
S
Department of Cell Biology, UMDNJ-SOM at Stratford, Stratford, NJ 08084, USA. serbor@aol.com
Lee
Jookyung
J
D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D008958:Models, Molecular; D011401:Promoter Regions, Genetic / Q000235:genetics; D011487:Protein Conformation; D014158:Transcription, Genetic
D002373:Cyclic AMP Receptor Protein; D004247:DNA; D012321:DNA-Directed RNA Polymerases