title: [Effects of exogenous nitric oxide donor on germination and activities of hydrolytic enzymes in wheat seed under osmotic stress].
abstract: Results in this paper showed that the effect of nitric oxide (NO) donor, sodium nitroprusside (SNP), which could promote the germination of wheat seeds under osmotic stress, was through enhancing imbibition using water content determination and imbibition analysis of wheat seed. SNP could also enhance amylase isozyme I activities, accelerate the liquefaction of endosperms, while have no effect on esterase. Moreover, SNP could strongly increase the sugars, such as the fructose, glucose and sucrose contents in wheat seeds during early germination (12 h) under normal conditions. When the seeds were treated with SNP with or without PTIO, an NO scavenger, and different exogenous sugars, i.e. fructose, glucose and sucrose, it was found that these sugars might activate amylase isozyme I activity mediated by the signal molecule NO to start the early stage of germination.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Sun
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Nie
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Shen
authors.forename: Lang-Lai
authors.initials: LL
authors.lastname: Xu
mesh_terms: D000681:Amylases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism; D018525:Germination / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014908:Triticum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D020030:Nitric Oxide Donors; D010940:Plant Proteins; D009599:Nitroprusside; D005632:Fructose; D013395:Sucrose; D000681:Amylases; D005947:Glucose