Evaluation of boar sperm maturation after co-incubation with caput, corpus and cauda epididymal cultures [corrected].
Boar sperm from the proximal caput epididymis were co-incubated with 1, 4, 7, 10 and 14-day old caput, corpus and cauda epididymal cultures for 24, 48 and 72 h. Boar kidney epithelial cells (LLC-PK1) and ECM alone were used as negative controls. Sperm motility, morphology and membrane integrity were studied to evaluate boar sperm maturation in vitro. Our results showed that epithelial cell monolayers (10, 14-day old) create a suitable microenvironment for the survival of proximal caput sperm and the maintenance of sperm motility over a 72 h period. Moreover, corpus epididymal tubule fragments in culture (1, 4-day old) are capable of promoting the migration of the cytoplasmic droplet along the sperm tail after 24h of co-incubation.
Bassols
J
J
Biotechnology of Porcine Reproduction, Department of Biology, INTEA, Faculty of Sciences, University of Girona, Campus Montilivi s/n, 17071 Girona, Spain.
Kádár
E
E
Briz
M
M
Pinart
E
E
Sancho
S
S
Garcia-Gil
N
N
Badia
E
E
Pruneda
A
A
Bussalleu
E
E
Yeste
M
M
Casas
I
I
Dacheux
J L
JL
Bonet
S
S
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D018920:Coculture Techniques; D004822:Epididymis / Q000166:cytology* / Q000502:physiology*; D004847:Epithelial Cells; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000254:growth & development* / Q000648:ultrastructure; D013552:Swine