title: Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction.
abstract: Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The I-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate.
authors.affiliation: Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8 Kita-ku, Sapporo 060-8628, Japan. takeda@nano.eng.hokudai.ac.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Takeda
authors.forename: Agus
authors.initials: A
authors.lastname: Sbagyo
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishii
authors.forename: Makoto
authors.initials: M
authors.lastname: Sawamura
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sueoka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.forename: Koichi
authors.initials: K
authors.lastname: Mukasa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics; D008839:Microelectrodes; D037742:Nanotubes, Carbon; D009532:Nickel / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D006388:Hemagglutinins; D037742:Nanotubes, Carbon; D009532:Nickel