Palestinian infantile visceral leishmaniasis caused by a genetic variant of Leishmania infantum belonging to a new zymodeme.
The parasites causing a Palestinian case of infantile visceral leishmaniasis (IVL) and those from four dogs from the Jenin District were identified serologically, biochemically and molecular biologically as Leishmania infantum, showing dogs act as a reservoir. The strain from the human case was distinct because of its unique 200-bp kDNA-polymerase chain reaction (PCR) component in its restriction fragment length polymorphism (RFLP) profile after digestion with the endonuclease RsaI, and by the electrophoretic mobility of its malate dehydrogenase (MDH(140)), designating it the reference strain of a new zymodeme of L. infantum, MON-281.
Bader
Khaldoun A
KA
Department of Parasitology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Schnur
Lionel F
LF
Nasereddin
Abedelmajeed
A
Pratlong
Francine
F
Dedet
Jean-Pierre
JP
Shaheen
Loay
L
Yousef
Obaida
O
Greenblatt
Charles L
CL
D000818:Animals; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis; D004197:Disease Reservoirs / Q000662:veterinary*; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000235:genetics / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
D016054:DNA, Protozoan