title: [Induction of protective immunity in rhesus monkey by inoculation with recombinant fusion protein of cholera toxin B subunit-multivalent epitopes of Plasmodium falciparum].
abstract: Rhesus monkeys (5 in each group) were inoculated with recombinant fusion protein of cholera toxin B subunit and multi-valent epitopes of Plasmodium falciparum intranasal or intramuscular (i.m.). Immune-responses and protective effect were evaluated. The antibody titer (Geometry mean) against CTB reached 1:512 (intranasal) and 1:10000 (i.m.) 14 day after 3rd immunization, and antibodies against P. falciparum were also elucidated, the titers in i.m. group were also significantly higher than that in intranasal group. The monkeys were challenged with 1.25 x 10(8) sporozoites of P. cynomolgi, Patent infection was observed in all 5 monkeys in control group inoculated with PBS in 10 - 14 days after challenge. Patent infection was also observed in 5 animals inoculated via intranasal and 2 animals in intramuscular group 19th days after challenge, But the infection last only 4 days in 3 animals in intranasal group and 2 animals in intramuscular group. The results demonstrated that the vaccine candidate could induce protective immune-responses in rhesus monkey against the challenge of P. cynomolgi.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100850, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhong
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Shi
authors.forename: Jie-Zhi
authors.initials: JZ
authors.lastname: Li
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Li
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Shi
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Ma
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000913:Antibodies, Protozoan / Q000097:blood; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology*; D004912:Erythrocytes / Q000469:parasitology; D008253:Macaca mulatta; D008288:Malaria / Q000517:prevention & control / Q000662:veterinary*; D017780:Malaria Vaccines / Q000276:immunology*; D008992:Monkey Diseases / Q000517:prevention & control*; D016789:Plasmodium cynomolgi; D010963:Plasmodium falciparum / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D017780:Malaria Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D002772:Cholera Toxin