Portal hypertension produces an evolutive hepato-intestinal pro- and anti-inflammatory response in the rat.
An inflammatory etiopathogeny can be suggested in portal hypertensive enteropathy since infiltration of the intestinal wall by mononuclear cells has been described in this condition. This work was carried out with the intention of shedding light on this matter. Male Wistar rats were divided into 4 control groups and 4 groups with partial portal vein ligation at 1, 2, 3 and 15 months. TNF-alpha, IL-1beta and IL-10 were quantified in liver and ileum by ELISA. CO and NO were measured in splanchnic and systemic vein by spectrophotometry and Griess reaction, respectively. Expression of constitutive and inducible isoforms of NO and HO were assayed by Western blot in liver and ileum. An increased hepatic release of proinflammatory mediators (TNF-alpha, IL-1beta and NO) associated with intestinal release of anti-inflammatory mediators (IL-10, CO) occurs in an early evolutive phase (1 month) of experimental portal hypertension. On the contrary, in the long-term (15 months), the increase in the intestinal release of proinflammatory mediators (TNF-alpha, IL-1beta) is associated with an increase in the hepatic release of anti-inflammatory mediators (IL-10, CO). These results suggest that experimental prehepatic portal hypertension presents changes in the serum and tissular (liver and small bowel) concentrations of mediators which are considered as pro- and anti-inflammatory.
Palma
Maria Dolores
MD
Surgery Chair, School of Medicine, Complutense University of Madrid, Spain.
Aller
Maria Angeles
MA
Vara
Elena
E
Nava
Maria Paz
MP
Garcia
Cruz
C
Arias-Diaz
Javier
J
Balibrea
Jose Luis
JL
Arias
Jaime
J
D000818:Animals; D002248:Carbon Monoxide / Q000097:blood; D003097:Collateral Circulation; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis; D006975:Hypertension, Portal / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D007082:Ileum / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D007249:Inflammation / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008642:Mesenteric Veins / Q000473:pathology / Q000503:physiopathology; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D002248:Carbon Monoxide; D019001:Nitric Oxide Synthase; D006419:Heme Oxygenase (Decyclizing)