Expression of developmentally regulated endothelial cell locus 1 was induced by tumor-derived factors including VEGF.
Developmentally regulated endothelial cell locus 1 (Del1) is a new angiogenic molecules expressed specifically in early embryonic endothelial cells. We investigated the relationship between Del1 and tumor cell-derived vascular endothelial growth factor (VEGF). Dunn osteosarcoma cells and high- and low-metastatic murine sarcoma cells did not express Del1. However, the expression of Del1 was observed in these primary tumor tissues and the pulmonary metastatic tissues after subcutaneous inoculation in vivo. Every tumor cell-conditioned medium containing VEGF induced the expression of Del1 in murine lung microvascular endothelial (MLE) cells, although control MLE cells did not express Del1. The anti-mouse VEGF monoclonal antibody inhibited the induction of the Del1 expression. In addition, mouse recombinant interleukin-1alpha and tumor necrosis factor-alpha also induced Del1 in MLE cells. Del1 may play an important role in tumor angiogenesis through the effects of tumor-derived factors including VEGF.
Aoki
Masato
M
Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, Toyama, Japan. aoki@med.kurobe.toyama.jp
Kanamori
Masahiko
M
Ohmori
Kazuo
K
Takaishi
Mikiro
M
Huh
Nam-ho
NH
Nogami
Shigeharu
S
Kimura
Tomoatsu
T
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics*; D015815:Cell Adhesion Molecules; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000166:cytology / Q000503:physiopathology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000502:physiology*
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D017077:Culture Media, Conditioned; D017931:DNA Primers; C491706:Edil3 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A