title: Alterations of glomerular and extracellular levels of glutathione peroxidase in patients and experimental rats with diabetic nephropathy.
abstract: To investigate the status and role of glutathione peroxidase (GPX) in diabetic nephropathy, we measured GPX in the plasma and urine of 14 patients with diabetic glomerulosclerosis (DGS) and measured glomerular GPX immunostaining in these patients and in rats with streptozotocin-induced diabetes of varying duration. Plasma GPX levels were significantly lower in DGS patients than in diabetic patients without nephropathy (P < .05) or normal controls (P < .01). Urinary GPX concentrations were also significantly lower in DGS patients than in diabetic patients without nephropathy or normal controls (both P < .05). Immunostaining of glomerular GPX was significantly less in DGS patients than in normal controls (P < .05) and was negatively correlated with the glomerular sclerosis score and the index of mesangial expansion. Serial examination of glomerular GPX in diabetic rats showed that immunostaining scores for glomerular GPX in rats were significantly lower than those in normal control rats after 1 and 3 months' duration of diabetes, and staining scores were also significantly lower in rats killed after 3 months of diabetes than in those killed after 1 week. In conclusion, our study demonstrates that GPX concentrations in plasma, urine, and glomeruli are decreased in individuals with DGS and that the immunostaining of glomerular GPX decreases progressively.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University, Taiwan.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chiu
authors.forename: Mei-Chuan
authors.initials: MC
authors.lastname: Kuo
authors.forename: Hung-Tien
authors.initials: HT
authors.lastname: Kuo
authors.forename: Jer-Ming
authors.initials: JM
authors.lastname: Chang
authors.forename: Jinn-Yuh
authors.initials: JY
authors.lastname: Guh
authors.forename: Yung-Hsiung
authors.initials: YH
authors.lastname: Lai
authors.forename: Hung-Chun
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000328:Adult; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D003928:Diabetic Nephropathies / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood* / Q000652:urine*; D006801:Humans; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005979:Glutathione Peroxidase