title: Quorum quenching enzyme activity is widely conserved in the sera of mammalian species.
abstract: Acyl-homoserine lactone (AHL) quorum sensing signals play a key role in synchronizing virulence gene expression in Pseudomonas aeruginosa, which could cause fatal bloodstream infections. We showed that AHL inactivation activity, albeit with variable efficiency, was conserved in the serum samples of all the 6 tested mammalian animals. High-performance liquid chromatography and mass spectrometry analyses revealed that mammalian sera had a lactonase-like enzyme(s), which hydrolyzed the lactone ring of AHL to produce acyl homoserine, with enzyme properties reminiscent of paraoxonases (PONs). We further showed that the animal cell lines expressing three mouse PON genes, respectively, displayed strong AHL degradation activities.
authors.affiliation: Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yi-Hu
authors.initials: YH
authors.lastname: Dong
authors.forename: Jiang Yong
authors.initials: JY
authors.lastname: Hu
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Zhang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism; D016466:CHO Cells; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity; D014774:Virulence
chemical_list: C088386:homoserine lactone; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D043303:Aryldialkylphosphatase; D015107:4-Butyrolactone