Effect of soybean lipoxygenase on volatile generation and inhibition of Aspergillus flavus mycelial growth.
Volatiles generated from lipoxygenase (LOX) normal and LOX deficient soybean (Glycine max) varieties with and without added lipase inhibited Aspergillus flavus mycelial growth and aflatoxin production. Soybean volatiles were analyzed using a solid phase microextraction (SPME) method combined with gas chromatography-mass spectrometry (GC-MS). Twenty-one compounds, including 11 aldehydes, three alcohols, four ketones, one furan, one alkane, and one alkene were detected in the LOX normal soybean line. However, only nine volatile compounds were observed in the LOX deficient soybean variety. The antifungal aldehydes hexanal and (E)-2-hexenal were observed in both LOX normal and LOX deficient lines and were detected at significantly higher amounts in soybean homogenate with added lipase. These aldehydes may be formed through alternate pathways, other than the LOX pathway, and may account for the inhibition of A. flavus growth observed. Other volatiles detected, particularly the ketones and alcohols, may contribute to the antifungal activity observed in both LOX normal and LOX deficient soybean lines. These results suggest that other factors, other than LOX activity, may better explain why soybeans are generally not as severely affected by A. flavus and aflatoxin contamination as other oilseed crops.
Boué
Stephen M
SM
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@srrc.ars.usda.gov
Shih
Betty Y
BY
Carter-Wientjes
Carol H
CH
Cleveland
Thomas E
TE
D000348:Aflatoxins / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism / Q000494:pharmacology; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology; D001231:Aspergillus flavus / Q000187:drug effects / Q000254:growth & development*; D008401:Gas Chromatography-Mass Spectrometry; D007659:Ketones / Q000378:metabolism / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism* / Q000494:pharmacology; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development*; D013025:Glycine max / Q000201:enzymology* / Q000382:microbiology; D014835:Volatilization
D000348:Aflatoxins; D000438:Alcohols; D000447:Aldehydes; D007659:Ketones; D008084:Lipoxygenase