title: [Study of IL-8 overexpression in A549 cells induced by crocidolite fibers].
abstract: OBJECTIVE
  This study was to investigate the effect of crocidolite fibers on IL-8 protein and mRNA levels in A549 cells cultured supernatant and the role of tyrosine kinase inhibitor PD98059 on IL-8 expression.
  
  
  METHODS
  Real Time PCR and ELISA were employed to detect A549 cellular IL-8 mRNA and supernatant IL-8 protein, respectively.
  
  
  RESULTS
  After incubating cells with 100 microg/ml of crocidolite, IL-8 protein releasing in supernatant was significantly increased and IL-8 mRNA was also higher than that of control, the differences were statistically significant (P < 0.05), however both IL-8 protein and mRNA was abrogated by pretreating cells with tyrosine kinase inhibitor PD98059.
  
  
  CONCLUSIONS
  IL-8 was shown as overexpression and could be decreased by PD98059. This indicated that MAPK signal pathway is involved in the process IL-8 expression.
authors.affiliation: School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xin-chao
authors.initials: XC
authors.lastname: Wang
authors.forename: Yu-bao
authors.initials: YB
authors.lastname: Xu
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Wu
authors.forename: Zhuo-wei
authors.initials: ZW
authors.lastname: Li
authors.forename: James M
authors.initials: JM
authors.lastname: Samet
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Ghio
mesh_terms: D017638:Asbestos, Crocidolite / Q000633:toxicity*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005419:Flavonoids; D016209:Interleukin-8; D012333:RNA, Messenger; D017638:Asbestos, Crocidolite; D011505:Protein-Tyrosine Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one