title: Theoretical calculations on hydrogenase kinetics: explanation of the lag phase and the enzyme concentration dependence of the activity of hydrogenase uptake.
abstract: Two models of the hydrogenase reaction cycle were investigated by means of theoretical calculations and model simulations. The first model is the widely accepted triangular hydrogenase reaction cycle with minor modifications; the second is a modified triangular model, where we have introduced an autocatalytic step into the reaction cycle. Both models include a one-step activation reaction. The theoretical calculations and model simulations corroborate the assumed autocatalytic reaction step concluded from the experimental characteristics of the hydrogenase reaction.
authors.affiliation: Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Osz
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bodó
authors.forename: Rui Miguel Mamede
authors.initials: RM
authors.lastname: Branca
authors.forename: Csaba
authors.initials: C
authors.lastname: Bagyinka
mesh_terms: D002384:Catalysis; D003901:Desulfovibrio / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006864:Hydrogenase / Q000737:chemistry*; D007700:Kinetics; D046911:Macromolecular Substances / Q000378:metabolism; D008956:Models, Chemical; D008962:Models, Theoretical; D012984:Software; D020616:Thiocapsa roseopersicina / Q000201:enzymology*; D013997:Time Factors
chemical_list: D046911:Macromolecular Substances; D006864:Hydrogenase