Identification and expression of a new type II transmembrane protein in human mast cells.
A cDNA encoding a new type II transmembrane protein has been isolated from human mast cells by subtraction cloning. This cDNA contains an open reading frame of 186 amino acids. RT-PCR analysis showed that this gene is differentially expressed in mast cells. Therefore, the peptide encoded by this gene was termed mast cell-expressed membrane protein 1 (MCEMP1). The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. Monoclonal antibodies against MCEMP1 were generated and characterized by immunoprecipitation and FACS. The results showed that the native MCEMP1 is expressed in cord blood-derived mast cells and HMC-1 and THP-1 cell lines, but not in other cell types that we have tested. Immunochemical staining of human lung sections showed that MCEMP1 staining is specifically associated with lung mast cells.
Li
Kang
K
Tanox, Inc., 10301 Stella Link Road, Suite 110, Houston, TX 77025, USA. kli@tanox.com
Wang
Shen-Wu
SW
Li
Yucheng
Y
Martin
Rachel E
RE
Li
Lixin
L
Lu
Meisheng
M
Lamhamedi-Cherradi
Salah-Eddine
SE
Hu
Guanghui
G
Demissie-Sanders
Sossiena
S
Zheng
Ji
J
Chung
Fan
F
Oates
Tim
T
Yao
Zhengbin
Z
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; C506757:MCEMP1 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins