The transcriptome of Populus in elevated CO2.
The consequences of increasing atmospheric carbon dioxide for long-term adaptation of forest ecosystems remain uncertain, with virtually no studies undertaken at the genetic level. A global analysis using cDNA microarrays was conducted following 6 yr exposure of Populus x euramericana (clone I-214) to elevated [CO(2)] in a FACE (free-air CO(2) enrichment) experiment. Gene expression was sensitive to elevated [CO(2)] but the response depended on the developmental age of the leaves, and < 50 transcripts differed significantly between different CO(2) environments. For young leaves most differentially expressed genes were upregulated in elevated [CO(2)], while in semimature leaves most were downregulated in elevated [CO(2)]. For transcripts related only to the small subunit of Rubisco, upregulation in LPI 3 and downregulation in LPI 6 leaves in elevated CO(2) was confirmed by anova. Similar patterns of gene expression for young leaves were also confirmed independently across year 3 and year 6 microarray data, and using real-time RT-PCR. This study provides the first clues to the long-term genetic expression changes that may occur during long-term plant response to elevated CO(2).
Taylor
Gail
G
School of Biological Sciences, Bassett Crescent East, University of Southampton, SO16 7PX, UK. g.taylor@soton.ac.uk
Street
Nathaniel R
NR
Tricker
Penny J
PJ
Sjödin
Andreas
A
Graham
Laura
L
Skogström
Oskar
O
Calfapietra
Carlo
C
Scarascia-Mugnozza
Giuseppe
G
Jansson
Stefan
S
D000222:Adaptation, Physiological; D001272:Atmosphere; D002245:Carbon Dioxide / Q000494:pharmacology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D032107:Populus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors
D002245:Carbon Dioxide