title: NG2 expressing cells in hippocampal cultures survive neurotoxic insult and retain the ability to divide.
abstract: It has been recently proposed that NG2 proteoglycan expressing cells may stand for a unique class of glia in adult CNS referred to as oligodendrocyte progenitor cells (OPCs). These NG2 positive cells can give rise to mature oligodendrocytes, however a large number of them persists in immature stage throughout a lifetime and responds to various types of injury. In order to investigate OPCs reactivity in vitro, a model of trimethyltin evoked neurodegeneration was used. The results demonstrate that NG2 expressing cells survive treatment with the neurotoxin in a concentration that injures most of neurons in the culture. Moreover, progenitors change their morphology when treated with trimethyltin, upregulate the NG2 proteoglycan expression and retain the ability to divide.
authors.affiliation: Laboratory of Mechanisms of Neurodegeneration and Neuroprotection, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland. kdzwonek@nencki.gov.pl
authors.forename: Karolina
authors.initials: K
authors.lastname: Dzwonek
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009498:Neurotoxins / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014298:Trimethyltin Compounds / Q000633:toxicity*; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D009498:Neurotoxins; D018809:Proliferating Cell Nuclear Antigen; D011509:Proteoglycans; D014298:Trimethyltin Compounds; D000099216:Chondroitin Sulfate Proteoglycan 4; C046488:trimethyltin