title: Effect of surface composition on the adsorption of photosystem I onto alkanethiolate self-assembled monolayers on gold.
abstract: We have used self-assembled monolayers (SAMs) prepared from omega-terminated alkanethiols on gold to generate model surfaces and examine the effect of surface composition on the adsorption of Photosystem I (PSI), stabilized in aqueous solution by Triton X-100. Triton-stabilized PSI adsorbs to high-energy surfaces prepared from HO- and HO2C-terminated alkanethiols but does not adsorb to low-energy surfaces. The inhibition of PSI adsorption at low-energy surfaces is consistent with the presence of a layer of Triton X-100 that adsorbs atop the hydrophobic SAM and presents a protein-resistant poly(ethylene glycol) (PEG) surface. While the presence of the PEG surface prevents the adsorption of PSI, the displacement of the inhibiting layer of Triton X-100 by dodecanol, a more active surfactant, greatly enhances the adsorption of PSI. This inhibiting effect by Triton X-100 can be extended to other protein systems such as bovine serum albumin.
authors.affiliation: Department of Chemical Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA.
authors.forename: B Samuel
authors.initials: BS
authors.lastname: Ko
authors.forename: Brian
authors.initials: B
authors.lastname: Babcock
authors.forename: G Kane
authors.initials: GK
authors.lastname: Jennings
authors.forename: S Grey
authors.initials: SG
authors.lastname: Tilden
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Peterson
authors.forename: David
authors.initials: D
authors.lastname: Cliffel
authors.forename: Elias
authors.initials: E
authors.lastname: Greenbaum