title: [Clonal study on single apoptotic cells in myelodysplastic syndromes].
abstract: OBJECTIVE
  To investigate the clonal origin of the apoptotic hematopoietic cells in myelodysplastic syndromes (MDS).
  
  
  METHODS
  Nineteen patients with MDS whose karyotypes had been analysed with G- or R-binding were studied. ISEL (DNA in situ end labelling) and FISH (fluorescence in situ hybridization) (simplified as ISEL/FISH) were performed simultaneously in a single nucleated cells on cytospin slides in 10/19 of the patients. The ratio of FISH positive cells to all nucleated cells and to apoptotic cells were counted. Eight slides from 4 normal donors induced apoptosis by Fas monoclonal antibody (McAb) were used as methodology control. For the rest 9 patients, different fractions of cells (PI-, Annexin V+PI-, Annexin V-PI-) were sorted by flow cytometry and then cytospined for FISH analysis (simplified as FCM/FISH).
  
  
  RESULTS
  The mean percentage of abnormal clonal cells in nuclear cells in the 10 cases detected by ISEL/FISH was 37.1%, whereas it was 24.0% in apoptotic cells. Induction by Fas McAb didn't lead to change of karyotype. Moreover, 8/9 patients analysed by FCM/FISH showed higher cell proportion with normal karyotypes in apoptosis-undergoing cells than that in non-apoptotic cells.
  
  
  CONCLUSIONS
  Over apoptotic hematopoietic cells in MDS are mainly residual normal hematopoietic cells.
authors.affiliation: Department of Hematology, the Sixth People's Hospital Affiliated to Jiaotong University, Shanghai 200233, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Shen
authors.forename: Quan
authors.initials: Q
authors.lastname: Pu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D019014:fas Receptor