Pathogenomics analysis of Leishmania spp.: flagellar gene families of putative virulence factors.
The trypanosomatid flagellar apparatus contains conventional and unique features, whose roles in infectivity are still enigmatic. Although the flagellum and the flagellar pocket are critical organelles responsible for all vesicular trafficking between the cytoplasm and cell surface, still very little is known about their roles in pathogenesis and how molecules get to and from the flagellar pocket. The ongoing analysis of the genome sequences and proteome profiles of Leishmania major and L infantum, Trypanosoma cruzi, T. brucei, and T. gambiensi ( www.genedb.org ), coupled with our own work on L. chagasi (as part of the Brazilian Northeast Genome Program- www.progene.ufpe.br ), prompted us to scrutinize flagellar genes and proteins of Leishmania spp. promastigotes that could be virulence factors in leishmaniasis. We have identified some overlooked parasite factors such as the MNUDC-1 (a protein involved in nuclear development and genomic fusion) and SQS (an enzyme of sterol biosynthesis), among the described flagellar gene families. A database concerning the results of this work, as well as of other studies of Leishmania and its organelles, is available at http://nugen.lcc.uece.br/LPGate . It will serve as a convenient bioinformatics resource on genomics and pathology of the etiological agents of leishmaniasis.
Oliveira
Diana M
DM
Núcleo de Genômica e Bioinformática, Faculdade de Veterinária, Universidade Estadual do Ceara (UECE), Campus do Itaperi, Fortaleza, Ceara, Brazil. nugen@lcc.uece.br
Gouveia
Joao Jose S
JJ
Diniz
Nilo B
NB
Pacheco
Ana Carolina L
AC
Vasconcelos
Elton Jose R
EJ
Diniz
Michely C
MC
Viana
Daniel A
DA
Ferreira
Thiago D
TD
Albuquerque
Marianna C
MC
Fortier
Daniel C
DC
Maia
Allan R S
AR
Costa
Luis A C
LA
Melo
João Osmar P
JO
da Silva
Maria Cristina
MC
Walter
Cezar A
CA
Faria
Jose O
JO
Tome
Adriana R
AR
Gomes
Marcos Jose N
MJ
Oliveira
Sonia M P
SM
Araújo-Filho
Raimundo
R
Costa
Raimundo B
RB
Maggioni
Rodrigo
R
D000595:Amino Acid Sequence; D000818:Animals; D030541:Databases, Genetic; D005407:Flagella / Q000235:genetics* / Q000502:physiology; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D007891:Leishmania / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D020543:Proteome; D015800:Protozoan Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics
D020543:Proteome; D015800:Protozoan Proteins