title: Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823.
abstract: OBJECTIVE
  To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823.
  
  
  METHODS
  The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line.
  
  
  RESULTS
  Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9+/-4.94% and 79.6+/-5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9+/-0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.
  
  
  CONCLUSIONS
  HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.
authors.affiliation: Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China. wxpphd@yahoo.com.cn
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liao
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Liu
authors.forename: Xing-Cui
authors.initials: XC
authors.lastname: Wang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Shang
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; C018551:glucose-regulated proteins