title: Chromosomal aberrations and frequency of micronuclei in sheep subchronically exposed to the fungicide Euparen Multi (tolylfluanid).
abstract: We analyzed chromosome aberrations, micronucleus frequency, mitotic index (MI), and nuclear division index (NDI) in peripheral lymphocytes of sheep subchronically exposed to the fungicide Euparen Multi (containing 50% tolylfluanid). Euparen Multi was administered by rumen sonde to group of Merino sheep (seven sheep/group) at 93 mg/kg body weight (1/20 LD50) daily for 28 days to assess its genotoxic effects. The frequencies of aberrant cells (ABC) in the experimental and control groups were 5.50+/-1.38% and 2.40+/-1.14%, respectively, and the increase in ABC in the treated group was significant (P = 0.003). Significantly increased numbers of chromatid breaks (5.67+/-1.21% against 2.40+/-1.14%; P = 0.001), chromatid gaps (10.33+/-2.73% against 4.00+/-1.23%; P = 0.001), and chromosome gaps (1.83+/-0.75% against 0.80+/-0.45%; P = 0.025) and exchanges (3.17+/-1.94% against 0.20+/-0.45%; P = 0.009) were observed in exposed animals in comparison to control animals. The frequency of micronuclei (MN) was 29.40+/-5.86 per 1000 binucleated cells in peripheral lymphocytes of sheep in the control group and 49.57+/-19.12 per 1000 binucleated cells in the treated group. A significant increase in the frequency of MN in peripheral lymphocytes also was observed between the two groups (P = 0.0477). No statistical differences in MI and NDI values were found in the groups (P = 0.181 and 0.761, respectively). Thus, our results suggest that exposure to Euparen Multi may cause genome damage in somatic cells.
authors.affiliation: University of Veterinary Medicine, Komenského 73, 041 81 Kosice, Slovak Republic. sutiakova@pobox.sk
authors.forename: Irena
authors.initials: I
authors.lastname: Sutiaková
authors.forename: Natália
authors.initials: N
authors.lastname: Kovalkovicová
authors.forename: Juraj
authors.initials: J
authors.lastname: Pistl
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Novotný
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Legáth
authors.forename: Gabriel
authors.initials: G
authors.lastname: Kovác
authors.forename: Sabina
authors.initials: S
authors.lastname: Hlincíková
authors.forename: Václav
authors.initials: V
authors.lastname: Sutiak
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D000935:Antifungal Agents / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced*; D005260:Female; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D012756:Sheep / Q000235:genetics; D013449:Sulfonamides / Q000633:toxicity*; D014052:Toluidines
chemical_list: D000814:Aniline Compounds; D000935:Antifungal Agents; D009153:Mutagens; D013449:Sulfonamides; D014052:Toluidines; C070021:N-dichlorofluoromethylthio-N',N'-dimethyl-N-p-tolylsulfamide