title: A new plasmid vector for regulated gene expression in Bacillus subtilis.
abstract: We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the P(R) promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage lambda. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.
authors.affiliation: Instituto de Biología Molecular Eladio Viñuela (CSIC), Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Serrano-Heras
authors.forename: Margarita
authors.initials: M
authors.lastname: Salas
authors.forename: Alicia
authors.initials: A
authors.lastname: Bravo
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D002874:Chromosome Mapping; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins