High-throughput proteomics using Fourier transform ion cyclotron resonance mass spectrometry.
The advent of high-throughput proteomic technologies for global detection and quantitation of proteins creates new opportunities and challenges for those seeking to gain greater understanding of the cellular machinery. Here, recent advances in high-resolution capillary liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry are reviewed along with its potential application to high-throughput proteomics. These technological advances combined with quantitative stable isotope labeling methodologies provide powerful tools for expanding our understanding of biology at the system level.
Qian
Wei-Jun
WJ
Biological Science Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA. weijun.qian@pnl.gov
Camp
David G
DG
Smith
Richard D
RD
D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation*; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*; D013997:Time Factors
D020543:Proteome