title: Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum.
abstract: Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and lambda(max) at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.
authors.affiliation: Department of Biology, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Subramanium
authors.initials: S
authors.lastname: Gopalakrishnan
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.forename: Jane L
authors.initials: JL
authors.lastname: Ward
authors.forename: Richard N
authors.initials: RN
authors.lastname: Strange
mesh_terms: D029863:Cicer / Q000382:microbiology*; D005670:Fusarium / Q000302:isolation & purification / Q000472:pathogenicity*; D009682:Magnetic Resonance Spectroscopy; D009285:Naphthoquinones / Q000302:isolation & purification / Q000633:toxicity*; D010935:Plant Diseases / Q000382:microbiology
chemical_list: C504634:8-O-methyl-fusarubin; D009285:Naphthoquinones