title: Anaesthesia-induced hyperglycaemia early in life is not predictive for development of diabetes in diabetes-prone BB rats.
abstract: Type 1 diabetes (T1D) is an autoimmune disease in genetically predisposed individuals characterised by selective destruction of the beta-cells. Development of diabetes is in the asymptomatic pre-diabetic period characterised by impaired first-phase insulin response and the first clinical symptom is elevated blood glucose (BG). It is still uncertain whether stress or incidental hyperglycaemia can be regarded as predictors for development of T1D or not, even when immunologic and genetic markers for T1D are considered. The aim of this study was to investigate if there was any relationship between elevated BG in 30-day-old anaesthetised pre-diabetic diabetes-prone Bio Breeding (BB-DP) rats and later development of diabetes. Rats anaesthetised by intraperitoneal (ip) injection for islet transplantation displayed significantly higher BG values (Delta1.27 mmol/l, p=8.27x10(-12)) compared to non-anaesthetised non-transplanted rats, indicating that ip injection and/or anaesthesia induce a higher BG level. Linear regression analysis of BG and time of onset of diabetes in transplanted and non-transplanted BB-DP rats revealed no correlation (R(2) at 0.0075 and 0.0324 and p-values at 0.56 and 0.23 respectively). We were not able to identify any association or correlation between the induced temporary hyperglycaemia in 30-day-old BB-DP rats and later development of diabetes.
authors.affiliation: Steno Diabetes Center, Gentofte, Denmark. tspa@novonordisk.com
authors.forename: T
authors.initials: T
authors.lastname: Sparre
authors.forename: A M
authors.initials: AM
authors.lastname: Ejrnaes Sprinkel
authors.forename: U
authors.initials: U
authors.lastname: Bjerre Christensen
authors.forename: A E
authors.initials: AE
authors.lastname: Karlsen
authors.forename: F
authors.initials: F
authors.lastname: Pociot
authors.forename: J
authors.initials: J
authors.lastname: Nerup
mesh_terms: D000758:Anesthesia / Q000009:adverse effects*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D006943:Hyperglycemia / Q000453:epidemiology / Q000209:etiology*; D011236:Prediabetic State; D015995:Prevalence; D051381:Rats; D011913:Rats, Inbred BB
chemical_list: D001786:Blood Glucose