title: Human placental explants in culture: approaches and assessments.
abstract: Placental explant cultures in vitro are useful for studying tissue functions including cellular uptake, production and release of secretory components, cell interactions, proliferation, growth and differentiation, gene delivery, pharmacology, toxicology, and disease processes. A variety of culture conditions are required to mimic in utero environments at different times of gestation including differing oxygen partial pressures, extracellular matrices and culture medium. Optimization of explant methods is examined for first and third trimester human placental tissue and the biological processes under investigation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642-8668, USA. richardk_miller@urmc.rochester.edu
authors.forename: R K
authors.initials: RK
authors.lastname: Miller
authors.forename: O
authors.initials: O
authors.lastname: Genbacev
authors.forename: M A
authors.initials: MA
authors.lastname: Turner
authors.forename: J D
authors.initials: JD
authors.lastname: Aplin
authors.forename: I
authors.initials: I
authors.lastname: Caniggia
authors.forename: B
authors.initials: B
authors.lastname: Huppertz
mesh_terms: D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D004338:Drug Combinations; D005109:Extracellular Matrix; D005260:Female; D006801:Humans; D007797:Laminin; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D011509:Proteoglycans
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen