title: Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions.
abstract: Despite the collective efforts of the international community to sequence the complete rice genome, telomeric regions of most chromosome arms remain uncharacterized. In this report we present sequence data from subtelomere regions obtained by analyzing telomeric clones from two 8.8 x genome equivalent 10-kb libraries derived from partial restriction digestion with HaeIII or Sau3AI (OSJNPb HaeIII and OSJNPc Sau3AI). Seven telomere clones were identified and contain 25-100 copies of the telomere repeat (CCCTAAA)(n) on one end and unique sequences on the opposite end. Polymorphic sequence-tagged site markers from five clones and one additional PCR product were genetically mapped on the ends of chromosome arms 2S, 5L, 10S, 10L, 7L, and 7S. We found distinct chromosome-specific telomere-associated tandem repeats (TATR) on chromosome 7 (TATR7) and on the short arm of chromosome 10 (TATR10s) that showed no significant homology to any International Rice Genome Sequencing Project (IRGSP) genomic sequence. The TATR7, a degenerate tandem repeat which is interrupted by transposable elements, appeared on both ends of chromosome 7. The TATR10s was found to contain an inverted array of three tandem repeats displaying an interesting secondary folding pattern that resembles a telomere loop (t-loop) and which may be involved in a protective function against chromosomal end degradation.
authors.affiliation: Brassica Genomics Team, National Institute of Agricultural Biotechnology, RDA, Suwon, 441-707, Korea.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Yang
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Song-Bin
authors.initials: SB
authors.lastname: Chang
authors.forename: Hans
authors.initials: H
authors.lastname: de Jong
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Oh
authors.forename: Sang-Nag
authors.initials: SN
authors.lastname: Ahn
authors.forename: Eric
authors.initials: E
authors.lastname: Fang
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D005822:Genetic Vectors; D018745:Genome, Plant; D015698:Genomic Library; D023281:Genomics; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D016615:Telomere / Q000235:genetics*
chemical_list: D018744:DNA, Plant