title: Improved protein identification through the use of unstained gels.
abstract: In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data.
authors.affiliation: Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Loiselle
authors.forename: William R
authors.initials: WR
authors.lastname: Thelin
authors.forename: Carol E
authors.initials: CE
authors.lastname: Parker
authors.forename: Nedyalka N
authors.initials: NN
authors.lastname: Dicheva
authors.forename: Barry A
authors.initials: BA
authors.lastname: Kesner
authors.forename: Viorel
authors.initials: V
authors.lastname: Mocanu
authors.forename: Frank
authors.initials: F
authors.lastname: Wang
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Milgram
authors.forename: Maria R Esteban
authors.initials: MR
authors.lastname: Warren
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D005782:Gels; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000592:standards; D012371:Robotics; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005782:Gels; D011506:Proteins