title: Covalent immobilization of lysozyme on stainless steel. Interface spectroscopic characterization and measurement of enzymatic activity.
abstract: A new strategy aiming at the protection of metallic surfaces against the growth of biofilms is presented here. This work reports the grafting of primary amines by aminosilanization of oxidized stainless steel followed by chemical coupling of the glycosidase lysozyme from hen egg white using glutaraldehyde as homobifunctional cross-linking agent. Controlled characterization of a stainless steel surface by X-ray photoelectron spectroscopy and Fourier transform infrared reflection-absorption spectroscopy at each step enabled the mode of binding, coverage, and orientation of the grafted molecules to be addressed. As a result, the stainless steel samples covered with a covalently immobilized layer of lysozyme showed some lytic activity on a suspension of bacteria Micrococcus lysodeikticus.
authors.affiliation: Ecole Nationale Supérieure de Chimie de Paris, Laboratoire de chimie et biochimie des complexes moléculaires, UMR CNRS 7576, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Minier
authors.forename: Michèle
authors.initials: M
authors.lastname: Salmain
authors.forename: Nadia
authors.initials: N
authors.lastname: Yacoubi
authors.forename: Lucica
authors.initials: L
authors.lastname: Barbes
authors.forename: Christophe
authors.initials: C
authors.lastname: Méthivier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Zanna
authors.forename: Claire-Marie
authors.initials: CM
authors.lastname: Pradier
mesh_terms: D000818:Animals; D002645:Chickens; D004531:Eggs; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D007202:Indicators and Reagents; D007700:Kinetics; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013193:Stainless Steel
chemical_list: D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D013193:Stainless Steel; D009113:Muramidase