title: A seroepidemiologic survey of canine visceral leishmaniosis among apparently healthy dogs in Croatia.
abstract: Cross-sectional investigation was done on seroprevalence of Leishmania sp. infection among apparently healthy dogs in an area where canine leishmaniosis is endemic. Survey included 68 dogs living in the coastal city of Split, and 238 dogs living in 12 villages scattered in the hinterland. Each dog was clinically examined for the presence of some discrete signs compatible with leishmaniosis and by dot-ELISA modification determined the presence of anti-Leishmania antibodies. The titre 1:600 and higher was regarded as positive in the study. The seroprevalence ranged from 0 to 42.85%, depending on the location. 54.34% of the seropositive dogs had moderately enlarged lymph nodes and/or some discrete changes on the skin. In our parasitological study, Leishmania sp. was isolated from several seropositive animals that had some clinical signs and from a few which did not have any. Data analysis revealed that serological positivity to Leishmania sp. was not associated with a dog's outdoor lifestyle and utility, but was associated with the gender and age.
authors.affiliation: Department for Parasitology and Parasitic Diseases, Veterinary Faculty, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia. tatjanaz@vef.hr
authors.forename: T
authors.initials: T
authors.lastname: Zivicnjak
authors.forename: F
authors.initials: F
authors.lastname: Martinković
authors.forename: A
authors.initials: A
authors.lastname: Marinculić
authors.forename: V
authors.initials: V
authors.lastname: Mrljak
authors.forename: N
authors.initials: N
authors.lastname: Kucer
authors.forename: V
authors.initials: V
authors.lastname: Matijatko
authors.forename: Z
authors.initials: Z
authors.lastname: Mihaljević
authors.forename: R
authors.initials: R
authors.lastname: Barić-Rafaj
mesh_terms: D000367:Age Factors; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D017523:Croatia / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs / Q000469:parasitology*; D019353:Endemic Diseases / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D018314:Leishmania infantum / Q000254:growth & development*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology* / Q000469:parasitology / Q000662:veterinary*; D016015:Logistic Models; D008297:Male; D015999:Multivariate Analysis; D012424:Rural Population; D016036:Seroepidemiologic Studies; D012737:Sex Factors; D011795:Surveys and Questionnaires; D014505:Urban Population
chemical_list: D000913:Antibodies, Protozoan