title: Intracoronary formation and retention of micro aggregates of leukocytes and platelets contribute to postischemic myocardial dysfunction.
abstract: OBJECTIVE
  Cardiac pump function and coronary regulation can be impaired after short-term ischemia. Recent studies with platelets (P) and neutrophils (PMN) yielded contradicting results about the "cellular" contribution to reperfusion injury.
  
  
  METHODS
  Isolated guinea pig hearts performing pressure-volume work were employed, external heart work (EHW), aortic flow (AF), coronary flow (CF) and heart rate (HR) serving as parameters of cardiac function. After global ischemia, human blood cells were given as bolus (1 min) during reperfusion (intracoronary hematocrit 7%). Expression of specific adhesion molecules (P: CD62P, CD41; PMN: integrin CD11b) was measured on cells before and after coronary passage (FACS analysis).
  
  
  RESULTS
  Postischemic recovery of pump function was significantly reduced in hearts with blood cell application (EHW: -cells 54 +/- 14%, +cells 41 +/-12%, p <0.05). Coronary response to bradykinin and reactive hyperemia were not effected. The blood-cell dependent functional loss was partly reduced by blocking CD18 (anti-CD 18) and completely abrogated by blockage of CD41 (lamifiban). The expression of CD11b on PMN and monocytes (M) and CD62P on platelets was significantly reduced in the coronary effluent and a significant decrease of CD41 on leukocytes occurred during coronary passage after ischemia. Increases in CD41 on PMN in the presence of lamifiban demasked intracoronary formation of micro aggregates (P/PMN). These micro aggregates were visualized by light microscopy. Electron microscopy revealed no significant microvascular plugging.
  
  
  CONCLUSIONS
  1) A specifically blood-cell induced loss of myocardial pump function has been demonstrated after short-term ischemia. 2) CD41 (= GpIIbIIIa) on P is responsible for this cardiac reperfusion damage. 3) The effect is causally linked to the formation of micro aggregates between PMN and P, but seems attenuated in the presence of erythrocytes as compared to effects reported from experiments in which PMN and P were applied singly or co-perfused. 4) Intracoronary retention of PMN, M and platelet-leukocyte micro aggregates seems to be transient, as adherence was not confirmed by electron microscopy.
authors.affiliation: Medizinische Klinik II, Universitätsklinikum Regensburg, Franz-Joseph-Strauss-Allee 11, 93053 Regensburg, Germany. angela.goetz@klinik-regensburg.de
authors.forename: A K
authors.initials: AK
authors.lastname: Götz
authors.forename: S
authors.initials: S
authors.lastname: Zahler
authors.forename: P
authors.initials: P
authors.lastname: Stumpf
authors.forename: U
authors.initials: U
authors.lastname: Welsch
authors.forename: B F
authors.initials: BF
authors.lastname: Becker
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D039481:CD11b Antigen / Q000032:analysis; D002449:Cell Aggregation; D003331:Coronary Vessels / Q000473:pathology* / Q000503:physiopathology; D004912:Erythrocytes / Q000502:physiology; D005434:Flow Cytometry; D006168:Guinea Pigs; D007962:Leukocytes / Q000502:physiology*; D008854:Microscopy, Electron; D009200:Myocardial Contraction; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology*; D040201:Platelet Membrane Glycoprotein IIb / Q000032:analysis
chemical_list: D039481:CD11b Antigen; D040201:Platelet Membrane Glycoprotein IIb