title: Highly fluorescent streptavidin-coated CdSe nanoparticles: preparation in water, characterization, and micropatterning.
abstract: CdSe quantum dots (QDs) with a high fluorescence quantum yield of 25% and a narrow size distribution were synthesized in a single step in water using glutathione as a stabilizing molecule. The exceptional optical properties enabled for the first time the detection of in-water-prepared single quantum dots at room temperature. For application as fluorescent bioanalytical probes, the QDs were coated with streptavidin. These QDs self-assemble with high contrast on micropatterned biotin while preserving their optical properties and their capability to bind in addition biotinylated molecules, a prerequisite for the development of novel supramolecular structures and bioassays.
authors.forename: Monika
authors.initials: M
authors.lastname: Bäumle
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stamou
authors.forename: Jean-Manuel
authors.initials: JM
authors.lastname: Segura
authors.forename: Ruud
authors.initials: R
authors.lastname: Hovius
authors.forename: Horst
authors.initials: H
authors.lastname: Vogel
mesh_terms: D001710:Biotin; D019187:Cadmium Compounds; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005978:Glutathione; D049329:Nanostructures; D045663:Quantum Dots; D018036:Selenium Compounds; D019809:Streptavidin; D014867:Water
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018036:Selenium Compounds; D014867:Water; D001710:Biotin; D019809:Streptavidin; C058667:cadmium selenide; D005978:Glutathione