High-density electrode array for imaging in vitro electrophysiological activity.
The development of a high-density active microelectrode array for in vitro electrophysiology is reported. Based on the Active Pixel Sensor (APS) concept, the array integrates 4096 gold microelectrodes (electrode separation 20 microm) on a surface of 2.5 mmx2.5 mm as well as a high-speed random addressing logic allowing the sequential selection of the measuring pixels. Following the electrical characterization in a phosphate solution, the functional evaluation has been carried out by recording the spontaneous electrical activity of neonatal rat cardiomyocytes. Signals with amplitudes from 130 microVp-p to 300 microVp-p could be recorded from different pixels. The results demonstrate the suitability of the APS concept for developing a new generation of high-resolution extracellular recording devices for in vitro electrophysiology.
Berdondini
L
L
Sensors, Actuators and Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland. luca.berdondini@unine.ch
van der Wal
P D
PD
Guenat
O
O
de Rooij
N F
NF
Koudelka-Hep
M
M
Seitz
P
P
Kaufmann
R
R
Metzler
P
P
Blanc
N
N
Rohr
S
S
D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation*; D006046:Gold; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000502:physiology; D051381:Rats
D006046:Gold