Synthesis of benzofurazan derivatization reagents for carboxylic acids and its application to analysis of fatty acids in rat plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry.
The derivatization regents for carboxylic acids, DAABD-AE (4-[2-(N,N-dimethylamino)ethylaminosulfonyl]7-(2-aminoethylamino)-2,1,3-benzoxadiazole), MePZBD-AE ([4-(4-N-methyl)piperazinosulfonyl]-7-(2-aminoethylamino)-2,1,3-benzoxadiazole) and APZBD-NHMe ([4-(4-N-aminoethyl)piperazinosulfonyl]-7-methylamino-2,1,3-benzoxadiazole) were developed for electrospray ionization-mass spectrometry (ESI-MS). The derivatization reaction with fatty acids was completed at 60 degrees C within 30 min. The derivatives of fatty acids were separated on a reversed-phase column and detected with ESI-MS. The detection limits attained for fatty acids were femtomol range and the calibration curves were linear over the range from 0.1 to 100 pmol (r2 > 0.992) for DAABD-AE and MePZBD-AE. DAABD-NHMe was applied to the analysis of fatty acids in rat plasma samples.
Tsukamoto
Yuhki
Y
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Santa
Tomofumi
T
Saimaru
Hiroshi
H
Imai
Kazuhiro
K
Funatsu
Takashi
T
D000818:Animals; D001572:Benzofurans / Q000138:chemical synthesis* / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D010069:Oxadiazoles / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D011921:Rats, Inbred WKY; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013449:Sulfonamides / Q000138:chemical synthesis / Q000737:chemistry
C507987:(4-(4-N-aminoethyl)piperazinosulfonyl)-7-methylamino-2,1,3-benzoxadiazole; C507986:(4-(4-N-methyl)piperazinosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole; C507985:4-(2-(N,N-dimethylamino)ethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole; D001572:Benzofurans; D002264:Carboxylic Acids; D005227:Fatty Acids; D007202:Indicators and Reagents; D010069:Oxadiazoles; D013449:Sulfonamides