Rapid assignment of protein side chain resonances using projection-reconstruction of (4,3)D HC(CCO)NH and intra-HC(C)NH experiments.
The reconstruction of higher-dimensional NMR spectra from projections can provide significant savings in instrument time. Here, we demonstrate its application to the (4,3)D HC(CCO)NH and intra-HC(C)NH experiments. The latter experiment contains a novel intra-residue filter element, which selectively correlates the side chain resonances with the corresponding intra-residue amide resonances. Compared to the conventional HC(C)NH experiment, the intra-HC(C)NH experiment reduces the spectral complexity and thus the minimum number of projections required for artifact-free reconstruction by half. The use of the projection-reconstruction technique allows rapid data collection and unambiguous assignment of aliphatic side chain nuclei at high resolution.
Jiang
Ling
L
Department of Biochemistry, Duke University Medical Center, 242 Nanaline Duke Building, Research Drive, Durham, NC 27710, USA.
Coggins
Brian E
BE
Zhou
Pei
P
D000465:Algorithms; D000577:Amides / Q000032:analysis* / Q000737:chemistry*; D000595:Amino Acid Sequence; D003198:Computer Simulation; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
D000577:Amides; C029152:G-substrate; D009419:Nerve Tissue Proteins; D011506:Proteins