title: Spoligotyping of Mycobacterium bovis isolates found in Manitoba.
abstract: Spoligotyping was applied to 44 isolates of Mycobacterium bovis obtained from the Canadian province of Manitoba. Isolates were obtained from submissions of elk (n = 16), deer (n = 1), and cattle (n = 27) tissues spanning the period of 1990 to early 2003. Two spoligotype profiles were obtained differing only in the reaction with oligonucleotide number 12. Forty of the 44 isolates (90.9%) hybridized with oligonucleotide 12 (MB-1 type), while the remaining 4 of 44 (9.1%) did not show a signal at position 12 (MB-2 type). Octal codes for these 2 types are 656573377603600 and 656473377603600, respectively. These spoligotypes have not been reported as occurring elsewhere worldwide.
authors.affiliation: Mycobacterial Diseases Centre of Expertise, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, Ontario. lwallacecy@inspection.gc.ca
authors.forename: Cyril
authors.initials: C
authors.lastname: Lutze-Wallace
authors.forename: Claude
authors.initials: C
authors.lastname: Turcotte
authors.forename: Melanie
authors.initials: M
authors.lastname: Sabourin
authors.forename: Gloria
authors.initials: G
authors.lastname: Berlie-Surujballi
authors.forename: Yvon
authors.initials: Y
authors.lastname: Barbeau
authors.forename: Dianne
authors.initials: D
authors.lastname: Watchorn
authors.forename: John
authors.initials: J
authors.lastname: Bell
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D003670:Deer / Q000382:microbiology*; D008350:Manitoba; D009163:Mycobacterium bovis / Q000145:classification*; D009841:Oligonucleotides / Q000032:analysis; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D009841:Oligonucleotides