title: [Quantitative studies of the production phase in rHSA fermentation].
abstract: The model equations of the production phase of rHSA fermentation were derived on the base of both elemental balance and metabolic balance, then the unknown parameters of the model were estimated by multivariable optimization. The possible reasons of discrepancy of production rate between different period of fermentation were discussed. The model could preferably described the relations between different macroscopic reaction rates of the process and keys for the high-efficiency expression of HAS were deduced.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, National Center of Biotechnology, Bioengineering College, East China University of Science and Technology, Shanghai 200237, China. huangmingzhi@263.net.cn
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Huang
authors.forename: Mei-Jin
authors.initials: MJ
authors.lastname: Guo
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Hang
authors.forename: Ying-Ping
authors.initials: YP
authors.lastname: Zhuang
authors.forename: Si-Liang
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D005285:Fermentation / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012709:Serum Albumin