Reduced platelet adhesion to titanium metal coated with apatite, albumin-apatite composite or laminin-apatite composite.
Titanium metal coated with apatite (HA-Ti), albumin-apatite composite (AA-Ti) or laminin-apatite composite (LA-Ti) was prepared by the immersion of NaOH- and heat-treated titanium metal in a calcium phosphate solution, or one supplemented with albumin or laminin. Platelet adhesion to the obtained materials under flow conditions was investigated in real time using a cone- and plate-type viscometer and fluorescence labeled platelets. Adhesion and activation of the platelets on the HA-Ti, AA-Ti and LA-Ti were definitely suppressed as compared with those on untreated titanium metal with a mirror surface. Furthermore, the numbers of platelets adhered to AA-Ti and LA-Ti are smaller than those adhered to HA-Ti, although the differences were not statistically significant. These findings suggest that HA-Ti, AA-Ti and LA-Ti, especially AA-Ti and LA-Ti, would exhibit thromboresistance that is superior to commercially pure titanium metal in terms of platelet adhesion.
Uchida
Masaki
M
Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, Tsukuba, Ibaraki, Japan. uchida-m@aist.go.jp
Ito
Atsuo
A
Furukawa
Katsuko S
KS
Nakamura
Keigo
K
Onimura
Yuji
Y
Oyane
Ayako
A
Ushida
Takashi
T
Yamane
Takashi
T
Tamaki
Tamotsu
T
Tateishi
Tetsuya
T
D000328:Adult; D000418:Albumins / Q000737:chemistry / Q000494:pharmacology*; D001031:Apatites / Q000737:chemistry*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D014025:Titanium / Q000737:chemistry*
D000418:Albumins; D001031:Apatites; D020099:Coated Materials, Biocompatible; D007797:Laminin; D014025:Titanium