Aberrations of 11q13 in laryngeal squamous cell lines and their prognostic significance.
Chromosomal aberrations were analyzed in 12 established cell lines derived from laryngeal squamous cell carcinoma. Cytogenetic and fluorescence in situ hybridization studies were used to identify aberrations in the 11q13 region and in some other chromosome regions. Amplification of 11q13 was established only in the cell lines derived from subjects with a survival period of less than 5 years and, together with the 3q gain, were the only chromosomal structural abnormalities connected with short survival. In this group we also found translocations with a breakpoint within 11q13. In three cell lines, 11q13 was observed as a homogenously staining region. The results suggest that amplification of 11q13, as well as re-arrangements potentially involved in up-regulation of the oncogenes mapped in 11q13, should be considered as markers of poor prognosis in laryngeal cancer. A diagnostic significance of 11q13 may be increased by a parallel determination with 3q gain.
Jarmuz
Małgorzata
M
Institute of Human Genetics, Polish Academy of Sciences, Institute of Human Genetics, ul. Strzeszynska 32, Poznan 60-479, Poland.
Grenman
Reidar
R
Golusinski
Wojciech
W
Szyfter
Krzysztof
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D019938:Cyclin D1 / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis
D019938:Cyclin D1