Comparison of different kinds of probes used for analysis of variant telomeric sequences.
In this work we aimed to compare and critically evaluate results obtained by different types of probes used for hybridisation to detect variant telomeric sequences with respect to their reliability and information value. Using slot-blot hybridisation we investigated three types of probes (oligonucleotides, cloned fragments and concatenated probes) under various conditions of hybridisation and washing. The concatenated probes exhibited the highest specificity although all three types are suitable for hybridisation of telomeric sequences under appropriate experimental conditions. We demonstrate how understanding generated from these data enables interpretation of hybridisation patterns of oligonucleotide probes to genomic DNAs.
Neplechová
Kamila
K
Department of Functional Genomics and Proteomics, Masaryk University Brno, Kotlárská 2, CZ-61137 Brno, Czech Republic.
Sýkorová
Eva
E
Fajkus
Jirí
J
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes / Q000032:analysis* / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D016678:Genome / Q000235:genetics; D006801:Humans; D016615:Telomere / Q000235:genetics*
D015342:DNA Probes