The immunoreactivity of systemic antibodies to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in adult periodontitis.
Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis secrete several potent virulence factors and are known to be two of the major periodontal pathogens. In the present case-control study, the systemic immunoreactivity to A. actinomycetemcomitans exotoxins, cytolethal distending toxin (Cdt) and leukotoxin was analyzed in adult subjects with periodontitis and in periodontally healthy controls. Furthermore, systemic immunoreactivity to P. gingivalis was analyzed in these subjects. Reactivity to the A. actinomycetemcomitans toxins was determined in bioassays that quantified neutralizing antibodies, and P. gingivalis antibodies were detected by enzyme-linked immunosorbent assay (ELISA). The results showed a significantly enhanced immunoreactivity to P. gingivalis in the subjects with periodontitis, while the reactivity to A. actinomycetemcomitans leukotoxin showed no significant difference between patients and controls. However, combined immunoreactivity to leukotoxin and Cdt was more prevalent in the subjects with periodontitis than in the controls. In addition, immunoreactivity to leukotoxin correlated to periodontitis in men but not in women. In conclusion, data from the present study indicate that immunoreactivity to P. gingivalis is frequent in adult periodontitis, while the role of A. actinomycetemcomitans seems to be more complex and depends on gender of the infected subject as well as the virulence of the bacteria.
Johansson
Anders
A
Department of Odontology/Periodontology, Faculty of Medicine, Umeå University, SE-901 87 Umeå, Sweden. anders.johansson@odont.umu.se
Buhlin
Kåre
K
Koski
Riikka
R
Gustafsson
Anders
A
D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood*; D001427:Bacterial Toxins / Q000276:immunology; D016022:Case-Control Studies; D003603:Cytotoxins / Q000276:immunology; D005098:Exotoxins / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D017622:Periodontal Attachment Loss / Q000276:immunology / Q000382:microbiology; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology*; D010519:Periodontium / Q000276:immunology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D012737:Sex Factors; D037521:Virulence Factors / Q000276:immunology
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003603:Cytotoxins; D005098:Exotoxins; D037521:Virulence Factors; C447141:cytolethal distending toxin, Actinobacillus actinomycetemcomitans; C018579:leukotoxin