[Karyotyping and immunophenotyping analyses of the CD34+ CD38- cells isolated from human umbilical cord blood].
OBJECTIVE
To cultivate hematopoietic stem/progenitor cells (CD34(+)CD38(-)) isolated from umbilical cord blood (UCB) long for the observation of cell growth and expansion in vitro, surface marker expression, and chromosomal complements.


METHODS
By flow cytometry CD34-FITC and CD38-PE labeled CD34(+) and CD38(-) stem/progenitor cells were isolated from UCB. The cells were cultivated in vitro for 6 months in a stem cell culture system with addition of six kinds of cell growth factors (IL-3, IL-6, GM-CSF, Epo, SCF, IGF-1). One month after cultivation, cultured cells were investigated for surface marker expression by flow cytometry and karyotype by G banding method.


RESULTS
After 7-12 days cultivation, the CD34(+)CD38(-) stem/progenitor cells began proliferation. The proliferation rate and the peak proliferation duration were greater in 1 cell/well cultivation conditions than in 10 cells/well. The cells remained CD34(+)CD38(-) and their karyotypic characteristics remained unchanged.


CONCLUSIONS
CD34(+)CD38(-) stem/progenitor cells from UCB may provide a larger than original amount of stem/progenitor cells for transplantation after long-term cultivation in vitro.
Tian
Hong
H
Department of Biology and Medical Genetics, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Zheng
Jin-e
JE
Gong
Fei-li
FL
Wang
Xing-bing
XB
Huang
Shi-ang
SA
Chen
Zhong
Z
D051997:ADP-ribosyl Cyclase 1 / Q000276:immunology; D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping
D018952:Antigens, CD34; D051997:ADP-ribosyl Cyclase 1