Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2.
OBJECTIVE
To investigate the pathological effect of Helicobacter pylori (H pylori) on human hepatic cells, proteomic methods were used to find and to identify proteins that were overexpressed in HepG2 cells treated by H pylori.


METHODS
H pylori was co-cultured with HepG2 for 6 h. Two-dimensional gel electrophoresis was used to gain the protein expression pattern of untreated and H pylori-treated HepG2. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry.


RESULTS
Seven proteins, which were up-regulated in H pylori-treated HepG2 cells, were identified. These proteins included integrin beta-1, protein kinase C alpha, LIM/homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, signal transduction, metabolism and so on.


CONCLUSIONS
H pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of some proteins.
Zhang
Yan
Y
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
Fan
Xue-Gong
XG
Chen
Ren
R
Xiao
Zhi-Qiang
ZQ
Feng
Xue-Ping
XP
Tian
Xue-Fei
XF
Chen
Zhao-Hui
ZH
D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D016481:Helicobacter Infections / Q000378:metabolism* / Q000503:physiopathology*; D016480:Helicobacter pylori; D006801:Humans; D008113:Liver Neoplasms; D013058:Mass Spectrometry; D040901:Proteomics; D015854:Up-Regulation