title: Determination of rizatriptan in human plasma by liquid chromatographic-eletrospray tandem mass spectrometry: application to a pharmacokinetic study.
abstract: A sensitive liquid chromatographic-tandem mass spectrometry(LC-MS/MS) method was developed for the determination of rizatriptan in human plasma. The analytes were extracted from plasma samples by liquid-liquid extraction, separated on a Zorbax XDB C8 column (150 x 4.6 mm i.d.) and detected by tandem mass spectrometry with an electrospray ionization interface. Zomitriptan was used as the internal standard. The method had a lower limit of quantitation of 50 pg/mL for rizatriptan, which showed more sensitivity and speed of analysis compared with reported methods. The within- and between-day precision was measured to be below 11.71% and accuracy between -5.87 and 0.86% for all quality control samples. This quantitation method was successfully applied to the evaluation of the pharmacokinetic profiles of rizatriptan after single oral administration of 5, 10 and 15 mg rizatriptan tablets to 10 healthy volunteers (five males and five females).
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, People's Republic of China. guojifen@sohu.com
authors.forename: Ji-fen
authors.initials: JF
authors.lastname: Guo
authors.forename: Ai-jun
authors.initials: AJ
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Zhao
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Gao
authors.forename: Ze-yuan
authors.initials: ZY
authors.lastname: Liu
authors.forename: Shan-yi
authors.initials: SY
authors.lastname: Qiao
mesh_terms: D002138:Calibration; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood* / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014230:Triazoles / Q000097:blood* / Q000493:pharmacokinetics; D014363:Tryptamines / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D017366:Serotonin Receptor Agonists; D014230:Triazoles; D014363:Tryptamines; C093622:rizatriptan