[Study of the expression membrane protein LASS2].
A human membrane protein LASS2 (Homo sapiens longevity assurance homologue 2 of yeast LAG1), which has important physiologic functions, was expressed in three different expression systems. Only the LASS2 protein carboxyl terminal hydrophilic fragment could be expressed in the prokaryote expression system and its polyclonal antibody was produced. The full length of LASS2 protein could be expressed successfully in both eukaryotic in vitro translation system and Bacuvirus expression system: Bac-to-Bac system. SDS-PAGE analysis revealed that the molecular weight of expressed product of LASS2 was about 28 kD. The product was also proved by Western blot. This recombinant LASS2 protein was purified by metal affinity resin and the purity is above 90%.
Cai
Xing-Feng
XF
Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
Tao
Zhen
Z
Cao
Yu
Y
Yang
Sheng-Li
SL
Gong
Yi
Y
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007313:Insecta; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051049:Sphingosine N-Acyltransferase; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; D025521:Tumor Suppressor Proteins; C438311:CERS2 protein, human; D051049:Sphingosine N-Acyltransferase