Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model.
OBJECTIVE
The 1,450-nm Smoothbeam Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non-ablative laser on Apligraf (bioengineered skin-substitute) and to document its use as a model for non-ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression.


METHODS
Three sheets of Apligraf were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10 J, 12J, and 14J. The sheets were then incubated with 10% CO(2) at 37 degrees C and samples were collected and analyzed 3 days later, using RT-PCR and immunofluorescent staining.


RESULTS
Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12 J.


CONCLUSIONS
There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J.
Nouri
Keyvan
K
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, 1475 NW 12th Avenue, Room 2175, Miami, FL 33136, USA. knouri@med.miami.edu
Zhang
Yan-Ping
YP
Singer
Lauren
L
Zhu
Linjian
L
Huo
Ran
R
Ricotti
Carlos
C
Prodanovich
Srdjan
S
Li
Jie
J
D003094:Collagen / Q000096:biosynthesis* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007231:Infant, Newborn; D028022:Low-Level Light Therapy / Q000379:methods*; D008954:Models, Biological; D017768:Skin, Artificial
C117898:Apligraf; D003094:Collagen