title: C-type lectin OCILRP2/Clr-g and its ligand NKRP1f costimulate T cell proliferation and IL-2 production.
abstract: We are reporting the identification of a novel C-type lectin receptor-ligand pair that is involved in T cell costimulation. The receptor, OCILRP2/Clr-g, is rapidly induced following T cell activation and maintained at a substantial level of up to 72 h. The ligand, NKRP1f, is predominantly expressed on dendritic cells (DC). The soluble OCILRP2-Ig blocking protein significantly suppresses specific antigen-stimulated T cell proliferation as well as IL-2 secretion both in vitro and in vivo; conversely, NKRP1f-expressing antigen presenting cells (APC) enhance B7.1/CD28-mediated costimulation for T cell proliferation through interaction with OCILRP2/Clr-g. Our studies reveal a unique functional interaction between two C-type lectins, OCILRP2/Clr-g and NKRP1f, during APC-mediated T cell costimulation and suggest a role for C-type lectins in maintaining T cell response or memory in vivo.
authors.affiliation: Division of Immunology, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Tian
authors.forename: Rafael
authors.initials: R
authors.lastname: Nunez
authors.forename: Shuhua
authors.initials: S
authors.lastname: Cheng
authors.forename: Yumei
authors.initials: Y
authors.lastname: Ding
authors.forename: Joseph
authors.initials: J
authors.lastname: Tumang
authors.forename: Clay
authors.initials: C
authors.lastname: Lyddane
authors.forename: Christopher
authors.initials: C
authors.lastname: Roman
authors.forename: Hsiou-Chi
authors.initials: HC
authors.lastname: Liou
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors
chemical_list: C470572:Dcl1 protein, mouse; D007376:Interleukin-2; D037181:Lectins, C-Type; D008024:Ligands; D008565:Membrane Proteins; C442138:Nkrp1f protein, mouse; D020033:Protein Isoforms; D011971:Receptors, Immunologic