Extraction of Mycobacterium tuberculosis DNA: a question of containment.
DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.
Somerville
Wendy
W
McGill University Health Centre Research Institute, Montreal, Quebec, Canada.
Thibert
Louise
L
Schwartzman
Kevin
K
Behr
Marcel A
MA
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003264:Containment of Biohazards; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D019286:Endopeptidase K / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012150:Polymorphism, Restriction Fragment Length; D012965:Sodium Chloride / Q000494:pharmacology
D002593:Cetrimonium Compounds; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012965:Sodium Chloride; D009113:Muramidase; D019286:Endopeptidase K; D000077286:Cetrimonium