title: Interleukin-4 induced down-regulation of skin homing receptor expression by human viral-specific CD8 T cells may contribute to atopic risk of cutaneous infection.
abstract: Factors controlling the expression of cutaneous lymphocyte-associated antigen (CLA) by T cells are poorly understood, but data from murine and human CD4(+) T cell systems have suggested that cytokines play an important role. However, there are no data examining the influence of cytokines on the expression of CLA by human antigen-specific CD8(+) T cells. Peripheral blood mononuclear cells (PBMC) were isolated from 10 HLA-A*0201-positive healthy individuals. Using HLA-peptide tetrameric complexes refolded with immunodominant peptides from Epstein-Barr virus (EBV), cytomegalovirus (CMV) and influenza A virus, we investigated the temporal associations of CLA expression by viral-specific CD8(+) T cells following stimulation with antigen. Ex vivo influenza matrix-specific CD8(+) T cells expressed significantly (P < 0.05) greater levels of CLA than EBV BMLF1 and CMV pp65-specific CD8(+) T cells (mean 9.7% influenza matrix versus 1.4% BMLF1 versus 1.1% pp65) and these differences were sustained on culture. However, regardless of viral specificity, interleukin (IL)-12 and IL-4 induced significant (P < 0.05) dose-dependent up-regulation and down-regulation of CLA expression, respectively, with IL-4 showing a dominant negative effect. In many cases, IL-4 resulted in complete abrogation of detectable CLA expression by the viral-specific CD8(+) T cells. Overall these data demonstrate that CLA expression by human viral-specific CD8(+) T cells is highly dynamic and that IL-4 causes significant down-regulation. Disorders associated with a type 2 cytokine shift may reduce the efficiency of skin homing by viral-specific CD8(+) T cells. Furthermore, the ability to modify the local and systemic microenvironment may offer novel therapeutic strategies that influence tissue-specific T cell homing.
authors.affiliation: Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
authors.forename: S L
authors.initials: SL
authors.lastname: Seneviratne
authors.forename: L
authors.initials: L
authors.lastname: Jones
authors.forename: A S
authors.initials: AS
authors.lastname: Bailey
authors.forename: R V
authors.initials: RV
authors.lastname: Samuel
authors.forename: A P
authors.initials: AP
authors.lastname: Black
authors.forename: G S
authors.initials: GS
authors.lastname: Ogg
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology; D003876:Dermatitis, Atopic / Q000150:complications / Q000276:immunology; D015536:Down-Regulation / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D012867:Skin / Q000276:immunology; D017193:Skin Diseases, Viral / Q000209:etiology / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; C063419:CTAGE1 protein, human; D018984:Epitopes, T-Lymphocyte; D008562:Membrane Glycoproteins; D016201:Receptors, Lymphocyte Homing; D018664:Interleukin-12; D015847:Interleukin-4