title: Porcine TCR CD3zeta-chain and eta-chain.
abstract: Complete porcine CD3zeta-chain cDNA sequence was obtained for the first time, and its genomic nucleotide sequence was investigated from exon 2 down to CD3eta-chain exon 8. The sequence of porcine CD3zeta-chain showed homologous amino acid sequence with human and murine counterparts, in contrast to CD3eta-chain exon 8 with diversity among animals previously investigated. CD3eta-chain peptide is an alternative splice form of CD3zeta-chain exon 7 splicing to CD3eta-chain exon 8 instead of CD3zeta-chain exon 8. The genomic sequences revealed that the splice acceptor sequences of CD3eta-chain exon 8 of all animals investigated to be completely uniform. Further, CD3eta-chain exon 8 amino acid sequences retained the unique characters of having high proline (Pro) and positively charged amino acid content except for rats and mice. Although the biological role of CD3eta-chain remains to be enigmatic, these evidences suggests the evolutional pressure to maintain its sequence.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumi-dori Amamiya-machi, Aoba, Sendai 981-8555, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Tamaki
authors.initials: T
authors.lastname: Isobe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Eguchi
authors.forename: Wei-Ran
authors.initials: WR
authors.lastname: Tang
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Amemiya
authors.forename: Junichiro
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Eimei
authors.initials: E
authors.lastname: Sato
authors.forename: Yohtaroh
authors.initials: Y
authors.lastname: Takagaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasue
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017252:CD3 Complex / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D011948:Receptors, Antigen, T-Cell; D013552:Swine / Q000235:genetics*
chemical_list: D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D018076:DNA, Complementary; D011948:Receptors, Antigen, T-Cell