Porcine TCR CD3zeta-chain and eta-chain.
Complete porcine CD3zeta-chain cDNA sequence was obtained for the first time, and its genomic nucleotide sequence was investigated from exon 2 down to CD3eta-chain exon 8. The sequence of porcine CD3zeta-chain showed homologous amino acid sequence with human and murine counterparts, in contrast to CD3eta-chain exon 8 with diversity among animals previously investigated. CD3eta-chain peptide is an alternative splice form of CD3zeta-chain exon 7 splicing to CD3eta-chain exon 8 instead of CD3zeta-chain exon 8. The genomic sequences revealed that the splice acceptor sequences of CD3eta-chain exon 8 of all animals investigated to be completely uniform. Further, CD3eta-chain exon 8 amino acid sequences retained the unique characters of having high proline (Pro) and positively charged amino acid content except for rats and mice. Although the biological role of CD3eta-chain remains to be enigmatic, these evidences suggests the evolutional pressure to maintain its sequence.
Yamamoto
Ryuji
R
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumi-dori Amamiya-machi, Aoba, Sendai 981-8555, Japan.
Isobe
Tamaki
T
Eguchi
Tomoko
T
Tang
Wei-Ran
WR
Kiyokawa
Nobutaka
N
Amemiya
Hiroshi
H
Fujimoto
Junichiro
J
Sato
Eimei
E
Takagaki
Yohtaroh
Y
Yasue
Hiroshi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017252:CD3 Complex / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D011948:Receptors, Antigen, T-Cell; D013552:Swine / Q000235:genetics*
D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D018076:DNA, Complementary; D011948:Receptors, Antigen, T-Cell