Suppressive effect of deoxynivalenol, a Fusarium mycotoxin, on bovine and porcine neutrophil chemiluminescence: an in vitro study.
We evaluated the immunotoxicity of deoxynivalenol (DON), a Fusarium mycotoxin, on bovine and porcine neutrophils in vitro by using two function parameters, luminol-dependent chemiluminescence and random migration under agarose. A 2-hr DON treatment suppressed the chemiluminescence of bovine and porcine cells by 42% and 35% (on average) at 10(-5)M, and by 19% and 26% at 10(-6)M. Slight suppression was observed at concentrations lower than 10(-6)M. However, after an 18-hr DON treatment, random migration of neutrophils of both species remained unaffected, even at the highest concentration (10(-5)M). Although further extensive studies are needed, to our knowledge this is the first study to have revealed in vitro that DON can affect neutrophil function.
Takayama
Hideko
H
Department of Safety Research, National Institute of Animal Health, 3-1-5 Kannon-dai, Tsukuba, Ibaraki 305-0856, Japan.
Shimada
Nobuaki
N
Mikami
Osamu
O
Murata
Hideo
H
D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D013552:Swine; D014255:Trichothecenes / Q000494:pharmacology*
D014255:Trichothecenes; D008165:Luminol; C007262:deoxynivalenol