title: Disruption and restoration of cell-cell junctions in mouse vestibular epithelia following aminoglycoside treatment.
abstract: The intracellular junction complexes, which consist of tight junctions (TJ), adherens junctions (AJ), and desmosomes, mediate cell-cell adhesion in epithelial cells. E-cadherin, which is a major component of AJ, plays a role not only in the maintenance of cell-cell junctions, but also in repressing cell proliferation. In this study, we examined changes of E-cadherin expression in mouse vestibular epithelia following local application of neomycin using immunohistochemistry and western blotting, and morphology of cell-cell junctions by transmission electron microscopy (TEM). Immunohistochemistry and western blotting revealed down-expression of E-cadherin and its consecutive recovery. TEM demonstrated temporal disruption of cell-cell junctions. Morphology of cell-cell junctions was more rapidly restored than recovery of E-cadherin expression. Transient disruption of cell-cell junctions and down-expression of E-cadherin is a rational response for the deletion of dying hair cells, and may be associated with a limited capacity for cell proliferations in mammalian vestibular epithelia following their rapid restoration.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Sakyo-ku, 606-8507 Kyoto, Japan. kim@ent.kuhp.kyoto-u.ac.jp
authors.forename: Tae-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kitajiri
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Endo
authors.forename: Shinji
authors.initials: S
authors.lastname: Takebayashi
authors.forename: Fukuichiro
authors.initials: F
authors.lastname: Iguchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kita
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tamura
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D022005:Adherens Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D064032:Calbindin 2; D016022:Case-Control Studies; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006319:Hearing Loss, Sensorineural / Q000139:chemically induced / Q000517:prevention & control; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D009355:Neomycin / Q000633:toxicity*; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D012444:Saccule and Utricle / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D019108:Tight Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015820:Cadherins; D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D009355:Neomycin