Integrative proteomics: functional and molecular characterization of a particular glutamate-related neuregulin isoform.
Glutamate is the major excitatory neurotransmitter in the mammalian brain and is related to memory by calcium-conducting receptors. Neuregulins have emerged as long-term modulating molecules of synaptic signaling by glutamate receptors, playing a role in some cognition/memory-related disorders and moreover being part of transient functional microdomains, called lipid rafts. Here we characterize one specific isoform of neuregulin as a central biomarker for glutamate-related signaling, integrating results from in vitro and in vivo models by a differential functional and proteomic approach.
Schillo
Simone
S
ProteoSys AG, Carl-Zeiss-Str. 51, 55129 Mainz, Germany.
Pejović
Vojislav
V
Hunzinger
Christian
C
Hansen
Torsten
T
Poznanović
Slobodan
S
Kriegsmann
Jörg
J
Schmidt
Werner J
WJ
Schrattenholz
André
A
D000544:Alzheimer Disease / Q000473:pathology; D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D007858:Learning; D009419:Nerve Tissue Proteins / Q000032:analysis; D020890:Neuregulin-1 / Q000032:analysis*; D020033:Protein Isoforms; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; C490580:Nrg1 protein, rat; D020033:Protein Isoforms; C117467:neuregulin beta; D018698:Glutamic Acid; D002118:Calcium