Effect of mechanical stress imposition on co-culture of hepatic parenchymal and nonparenchymal cells: possibility of stimulating production of regenerating factor.
The effect of mechanical stress generated within a three-dimensional bioreactor on the co-culture of hepatic parenchymal cells (PC) and hepatic nonparenchymal cells (NPC) was assessed to develop a bioartificial liver that can produce factors accelerating liver regeneration. A rotating radial flow bioreactor was used to provide mechanical stress to a co-culture of PC and NPC that were isolated from rats. They were co-cultured in the reactor under static or dynamic conditions. Albumin, interleukin-6 (IL-6), hepatocyte growth factor (HGF), and lactate dehydrogenase (LDH) were measured at intervals. Electron microscopy was also performed. LDH was not significantly different between the static and mechanical stress-loaded cultures, while albumin and interleukin-6 levels were higher in the latter at all sampling times. Only the co-cultures loaded with mechanical stress produced HGF in the early stage of culture (hours 3 and 6). Histologically, the cells retained their structure when cultured under dynamic conditions. These results suggested that an appropriate level of mechanical stress enabled co-cultures of PC and NPC to produce IL-6, HGF, and other factors that accelerate liver regeneration.
Miyazawa
M
M
Department of Surgery, Saitama Medical School, 38-Morohongou, Moroyama, Iruma-gun, Saitama 350-0495, Japan.
Torii
T
T
Toshimitsu
Y
Y
Koyama
I
I
D018920:Coculture Techniques; D017228:Hepatocyte Growth Factor / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D012709:Serum Albumin / Q000032:analysis; D013314:Stress, Mechanical
D015850:Interleukin-6; D012709:Serum Albumin; D017228:Hepatocyte Growth Factor; D007770:L-Lactate Dehydrogenase