title: [Ginsenoside Rbl attenuates beta-amyloid peptide25-35 -induced tau hyperphosphorylation in cortical neurons].
abstract: OBJECTIVE
  To explore the effect and the possible mechanism of ginsenoside Rb1 on beta-amyloid peptide (beta-AP)(25-35) -induced tau protein hyperphosphorylation in cortical neurons.
  
  
  METHODS
  Western blotting and immunocytochemical staining were used to detect tau phosphorylation level, total tau and glycogen synthase kinase-3beta (GSK-3beta) in cortical neurons.
  
  
  RESULTS
  After exposure to beta-AP(25-35) (20 micromol x L(-1)) for 12 h, the levels of tau protein phosphorylation in the sites of Ser 396, Ser 199/202, Thr 231 and total tau were raised. Meanwhile, the expression of GSK-3beta also increased. Pretreatment with ginsenoside Rbl or lithium chloride, a specific inhibitor of GSK-3beta, markedly reduced beta-AP(25-35)-induced tau hyperphosphorylation and the expression of GSK-3beta.
  
  
  CONCLUSIONS
  Ginsenoside Rb1 can attenuate beta AP(25-35)-induced tau protein hyperphosphorylation in cortical neurons by inhibiting the expression of GSK-3beta.
authors.affiliation: Fujian Institute of Geriatrics, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
authors.forename: Yu-qi
authors.initials: YQ
authors.lastname: Zeng
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Yuan-gui
authors.initials: YG
authors.lastname: Zhu
authors.forename: Yong-kun
authors.initials: YK
authors.lastname: Li
authors.forename: Xiao-song
authors.initials: XS
authors.lastname: Peng
authors.forename: Li-min
authors.initials: LM
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Tian-wen
authors.initials: TW
authors.lastname: Huang
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D009474:Neurons / Q000378:metabolism; D005894:Panax / Q000737:chemistry; D010446:Peptide Fragments / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D036145:Ginsenosides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D016875:tau Proteins; C442759:ginsenoside Rb1; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3