[Clonal study on single apoptotic cells in myelodysplastic syndromes].
OBJECTIVE
To investigate the clonal origin of the apoptotic hematopoietic cells in myelodysplastic syndromes (MDS).


METHODS
Nineteen patients with MDS whose karyotypes had been analysed with G- or R-binding were studied. ISEL (DNA in situ end labelling) and FISH (fluorescence in situ hybridization) (simplified as ISEL/FISH) were performed simultaneously in a single nucleated cells on cytospin slides in 10/19 of the patients. The ratio of FISH positive cells to all nucleated cells and to apoptotic cells were counted. Eight slides from 4 normal donors induced apoptosis by Fas monoclonal antibody (McAb) were used as methodology control. For the rest 9 patients, different fractions of cells (PI-, Annexin V+PI-, Annexin V-PI-) were sorted by flow cytometry and then cytospined for FISH analysis (simplified as FCM/FISH).


RESULTS
The mean percentage of abnormal clonal cells in nuclear cells in the 10 cases detected by ISEL/FISH was 37.1%, whereas it was 24.0% in apoptotic cells. Induction by Fas McAb didn't lead to change of karyotype. Moreover, 8/9 patients analysed by FCM/FISH showed higher cell proportion with normal karyotypes in apoptosis-undergoing cells than that in non-apoptotic cells.


CONCLUSIONS
Over apoptotic hematopoietic cells in MDS are mainly residual normal hematopoietic cells.
Li
Xiao
X
Department of Hematology, the Sixth People's Hospital Affiliated to Jiaotong University, Shanghai 200233, China.
Shen
Wei-ming
WM
Pu
Quan
Q
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020287:In Situ Nick-End Labeling; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000378:metabolism
D019014:fas Receptor