title: Immunoblot analysis of specific antigen bands predictable for Dirofilaria immitis infection in cats.
abstract: Serial sera from four mongrel cats experimentally inoculated with infectious larvae of Dirofilaria immitis were analyzed by immunoblot patterns against a phosphate buffered saline-extract of D. immitis. Antigen-specific protein bands detected indicate that the low molecular weight bands of 36, 32, 22, 19 and 14 kDa, are predictable for positive adult worm infection, suggesting diagnostic usefulness for adult D. immitis infection in cats.
authors.affiliation: Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. hayasaki@yamaguchi-u.ac.jp
authors.forename: Mineo
authors.initials: M
authors.lastname: Hayasaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Mori
authors.forename: Haruka
authors.initials: H
authors.lastname: Hongo
authors.forename: Akiyo
authors.initials: A
authors.lastname: Katsuya
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Song
authors.forename: Satoshi
authors.initials: S
authors.lastname: Une
authors.forename: Tadao
authors.initials: T
authors.lastname: Ikeda
authors.forename: Hong-Kean
authors.initials: HK
authors.lastname: Ooi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uchida
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000097:blood*; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D002415:Cats; D004183:Dirofilaria immitis / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D005260:Female; D008297:Male
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth