title: [Prevalence of Escherichia coli strains in the canteens].
abstract: The prevalence of enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) E. coli strains in stool specimens from asymptomatic human carriers working in the canteens and also in the kitchen and sanitary facilities was evaluated. The E. coli genes coding for the following virulence markers: intimin (eae), enterohaemolysin (hlyA), and verotoxins type I and II (stx1 and stx2) were sought by multiplex PCR assay. E. coli isolates were obtained from 144 stool specimens, 295 swabs taken from kitchen hardware and surrounding facilities, and from 33 meat specimens. Only 66 (8.5%) of total 777 E. coli isolates belonged to O44, O18, O25, O127, O55, O114, O125, and O142 serogroups, the prevalent serogroups in Poland. None of the strains was classified as serogroup O157. The serogroups O44 and O18 were present most often among all typeable strains and their incidence was 51.5% and 25.8% respectively. Among 363 isolates assayed for the presence of the genes encoding virulence markers only 10 isolates (2.8%) carried eae gene. None of the isolates possessing eae gene belonged to the serogroups tested. The hlyA, stx1 and stx2 genes were absent in all E. coli isolates tested.
authors.affiliation: Zakład Mikrobiologii i Epidemiologii, Wojskowego Instytutu Higieny i Epidemiologii w Warszawie.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Szpakowska
authors.forename: Elzbieta A
authors.initials: EA
authors.lastname: Trafny
authors.forename: Krystyna
authors.initials: K
authors.lastname: Kozłowska
authors.forename: Jacek
authors.initials: J
authors.lastname: Grzybowski
mesh_terms: D001427:Bacterial Toxins / Q000302:isolation & purification*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity*; D006801:Humans; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D001427:Bacterial Toxins