ZNF328, a novel human zinc-finger protein, suppresses transcriptional activities of SRE and AP-1.
The zinc finger proteins containing the Kruppel-associated box domain (KRAB-ZFPs) are the single largest class of transcription factors in human genome. Many of the KRAB-ZFPs are involved in cardiac development or cardiovascular diseases. Here, we have identified a novel human KRAB zinc finger gene, named ZNF328, from the human fetal heart cDNA library. The complete sequence of ZNF328 cDNA contains a 2376-bp open reading frame (ORF) and encodes a 792 amino acid protein with an N-terminal KRAB domain and classical zinc finger C2H2 motifs in the C-terminus. Northern blot analysis indicates that the protein is expressed in most of the examined human adult and embryonic tissues. ZNF328 is a transcription suppressor when fused to Gal-4 DNA-binding domain and cotransfected with VP-16. Overexpression of ZNF328 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1. Deletion analysis with a series of truncated fusion proteins indicates that the KRAB motif is a basal repression domain when cotransfected with VP-16. Similar results were obtained when the truncated fusion proteins were assayed for the transcriptional activities of SRE and AP-1. These results suggest that ZNF328 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.
Ou
Ying
Y
The Center for Heart Development, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People's Republic of China.
Wang
Shenqiu
S
Cai
Zhenyu
Z
Wang
Yuequn
Y
Wang
Canding
C
Li
Yongqing
Y
Li
Fang
F
Yuan
Wuzhou
W
Liu
Bisheng
B
Wu
Xiushan
X
Liu
Mingyao
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors* / Q000502:physiology; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C502774:SRE protein, human; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C504030:ZNF606 protein, human