Two-photon excitation in fluorescence polarization receptor-ligand binding assay.
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.
Tirri
Marko E
ME
Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland. marko.tirri@lfy.utu.fi
Huttunen
Roope J
RJ
Toivonen
Juha
J
Härkönen
Pirkko L
PL
Soini
Juhani T
JT
Hänninen
Pekka E
PE
D005454:Fluorescence Polarization; D008024:Ligands; D017785:Photons; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000378:metabolism*
D008024:Ligands; D011960:Receptors, Estrogen