Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part III. Hydrogels as carriers for immobilization of proteins.
Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)-sodium methacrylate (MANa), copolymer HEMA-[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA-MANa-MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.
Michálek
J
J
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic. jiri@imc.cas.cz
Prádný
M
M
Artyukhov
A
A
Slouf
M
M
Smetana
K
K
D000327:Adsorption; D000418:Albumins / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D001360:Avidin / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D020100:Hydrogels / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D008422:Materials Testing; D008689:Methacrylates / Q000032:analysis / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D013499:Surface Properties
D000418:Albumins; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D008689:Methacrylates; D001360:Avidin; C005044:hydroxyethyl methacrylate