title: A physiological product-release model for baculovirus infected insect cells.
abstract: Existing models describe the product release from baculovirus infected insect cells as an unspecific protein leakage occurring in parallel with protein production. The model presented here shows that the observed product release of normally non-secreted proteins can be described through cell death alone. This model avoids the implicit non-physiological assumption of previous models that cells permeable to recombinant protein as well as trypan blue continue to produce protein.
authors.affiliation: Laboratory for Biological Engineering, Department of Chemical Engineering, the University of Queensland, 4072 Brisbane, Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Haas
authors.forename: Lars K
authors.initials: LK
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D007313:Insecta / Q000166:cytology*; D008962:Models, Theoretical; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology / Q000235:genetics
chemical_list: D011994:Recombinant Proteins