[Quantitative studies of the production phase in rHSA fermentation].
The model equations of the production phase of rHSA fermentation were derived on the base of both elemental balance and metabolic balance, then the unknown parameters of the model were estimated by multivariable optimization. The possible reasons of discrepancy of production rate between different period of fermentation were discussed. The model could preferably described the relations between different macroscopic reaction rates of the process and keys for the high-efficiency expression of HAS were deduced.
Huang
Ming-Zhi
MZ
State Key Laboratory of Bioreactor Engineering, National Center of Biotechnology, Bioengineering College, East China University of Science and Technology, Shanghai 200237, China. huangmingzhi@263.net.cn
Guo
Mei-Jin
MJ
Chu
Ju
J
Hang
Hai-Feng
HF
Zhuang
Ying-Ping
YP
Zhang
Si-Liang
SL
D005285:Fermentation / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D012709:Serum Albumin