title: PCR-generated artefact from 16S rRNA gene-specific primers.
abstract: Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex microbial communities, and after amplification from DNA from a microbial culture. Similar repetitive motifs were found in gene sequences deposited in GenBank. The artefact can be avoided by using different primers for the amplification reaction.
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Osborne
authors.forename: Maja
authors.initials: M
authors.lastname: Galic
authors.forename: Parveen
authors.initials: P
authors.lastname: Sangwan
authors.forename: Peter H
authors.initials: PH
authors.lastname: Janssen
mesh_terms: D000818:Animals; D016477:Artifacts; D017931:DNA Primers; D007082:Ileum / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S