Applications of two-photon microscopy in the neurosciences.
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.
Cruz
Hans G
HG
Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
Lüscher
Christian
C
D001931:Brain Mapping; D008853:Microscopy; D008856:Microscopy, Fluorescence / Q000639:trends*; D009474:Neurons / Q000502:physiology*; D009488:Neurosciences / Q000379:methods*; D038761:Photobleaching; D017785:Photons