title: Cell Cycle Arrest by a Natural Product via G2/M Checkpoint.
abstract: CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3sigma (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3sigma in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.
authors.affiliation: Technology Development, CK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong Kong, China.
authors.forename: Sharon Chui-Wah
authors.initials: SC
authors.lastname: Luk
authors.forename: Stephanie Wing-Fai
authors.initials: SW
authors.lastname: Siu
authors.forename: Chun-Kit
authors.initials: CK
authors.lastname: Lai
authors.forename: Ying-Jye
authors.initials: YJ
authors.lastname: Wu
authors.forename: Shiu-Fun
authors.initials: SF
authors.lastname: Pang