Cell Cycle Arrest by a Natural Product via G2/M Checkpoint.
CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3sigma (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3sigma in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.
Luk
Sharon Chui-Wah
SC
Technology Development, CK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong Kong, China.
Siu
Stephanie Wing-Fai
SW
Lai
Chun-Kit
CK
Wu
Ying-Jye
YJ
Pang
Shiu-Fun
SF