Short acidic peptides isolated from wheat sprout chromatin and involved in the control of cell proliferation. Characterization by infrared spectroscopy and mass spectrometry.
Low molecular weight peptides were isolated from the chromatin of wheat sprouts. Following gel filtration the peptide fraction shows a sharp inhibiting activity on the growth of HeLa cancer cells. Infrared (IR) spectroscopy and mass spectrometry have been utilized to characterize the wheat sprout peptides in an attempt to recognize the peptide sequence involved in the control of cell growth. The quantitative presence of a peptide with MH+=572 appears proportional to the cell growth inhibition activity. This compound has been subjected to extensive mass spectrometry analysis. The automatic computational analysis of the ions of second, third and fourth generations indicate a peptide sequence, AcHis-Asp-Ser-Glu-, that binds at the C-terminal a molecule of ethanolamine. Moreover, the results show that some sequences of the wheat sprout peptide family are present in the peptide fractions isolated from several other tissues, thus supporting the hypothesis of ubiquitous regulatory peptides.
Calzuola
Isabella
I
Dipartimento di Biologia Cellulare e Molecolare, Università degli Studi di Perugia, 06123 Perugia, Italy.
Giavarini
Flavio
F
Sassi
Paola
P
De Angelis
Leonardo
L
Gianfranceschi
Gian Luigi
GL
Marsili
Valeria
V
D049109:Cell Proliferation / Q000187:drug effects*; D002843:Chromatin / Q000737:chemistry*; D019856:Ethanolamine / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020539:Sequence Analysis, Protein; D014908:Triticum / Q000737:chemistry*
D002843:Chromatin; D010455:Peptides; D010940:Plant Proteins; D019856:Ethanolamine