Effect of salicylate on the elasticity, bending stiffness, and strength of SOPC membranes.
Salicylate is a small amphiphilic molecule which has diverse effects on membranes and membrane-mediated processes. We have utilized micropipette aspiration of giant unilamellar vesicles to determine salicylate's effects on lecithin membrane elasticity, bending rigidity, and strength. Salicylate effectively reduces the apparent area compressibility modulus and bending modulus of membranes in a dose-dependent manner at concentrations above 1 mM, but does not greatly alter the actual elastic compressibility modulus at the maximal tested concentration of 10 mM. The effect of salicylate on membrane strength was investigated using dynamic tension spectroscopy, which revealed that salicylate increases the frequency of spontaneous defect formation and lowers the energy barrier for unstable hole formation. The mechanical and dynamic tension experiments are consistent and support a picture in which salicylate disrupts membrane stability by decreasing membrane stiffness and membrane thickness. The tension-dependent partitioning of salicylate was utilized to calculate the molecular volume of salicylate in the membrane. The free energy of transfer for salicylate insertion into the membrane and the corresponding partition coefficient were also estimated, and indicated favorable salicylate-membrane interactions. The mechanical changes induced by salicylate may affect several biological processes, especially those associated with membrane curvature and permeability.
Zhou
Yong
Y
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA.
Raphael
Robert M
RM
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects / Q000494:pharmacology; D001703:Biophysics / Q000379:methods*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004548:Elasticity; D004563:Electrochemistry; D007249:Inflammation; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D008956:Models, Chemical; D015233:Models, Statistical; D010713:Phosphatidylcholines / Q000737:chemistry*; D012459:Salicylates / Q000737:chemistry; D020156:Salicylic Acid / Q000009:adverse effects* / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013053:Spectrophotometry; D013816:Thermodynamics; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D012459:Salicylates; D012709:Serum Albumin; C027891:1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine; D020156:Salicylic Acid