Voluntary wheel-running exercise enhances antigen-specific antibody-producing splenic B cell response and prolongs IgG half-life in the blood.
Exercise has been recognized to provoke upregulation of antibodies. However, the mechanism has not been explained. We examined the effects of voluntary wheel-running exercise on the number of cells which produce tetanus toxoid (TT)-specific IgG, as well as serum level and clearance of administered 125I-labeled mouse IgG in the blood. Male C57BL/6N mice were randomly divided into a voluntary wheel-running exercise group and a sedentary group. Mice were intraperitoneally immunized with 0.375 microg/kg of TT to induce primary and secondary anti-TT antibody responses. ELISPOT assays that identified TT-specific antibody production were performed on day 0 (Baseline, n = 8) and 22 (EX: n = 8, Non-EX: n = 8) after initial immunization (primary response) and on day 32 (EX: n = 8, Non-EX: n = 7) and 43 (EX: n = 7, Non-EX: n = 7). To explain why serum TT-specific IgG was elevated in the exercise group, we conducted an 125I-labeled mouse IgG clearance test on day 32. ELISPOT counts of secondary responses to TT immunization were significantly higher in the running group than in the sedentary group (P<0.05). The serum anti-TT specific IgG concentration was also significantly higher in the running group (P<0.05) than in the sedentary on day 32. The values of both groups were relatively lower on day 43. The (125)I-labeled mouse IgG was more rapidly cleared in the non-exercised than in the exercised group (P<0.05). These results show that voluntary wheel running upregulates the TT-specific humoral immune response. These reactions may be partly explained by the accelerated induction of TT-specific IgG-producing cells and prolonged serum IgG half-life with voluntary exercise.
Suzuki
Koutarou
K
Laboratory of Exercise and Environmental Health, Division of Health and Sport Sciences, Postgraduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8574, Japan.
Tagami
Kazumi
K
D000222:Adaptation, Physiological / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D006207:Half-Life; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology*; D010805:Physical Conditioning, Animal; D005082:Physical Exertion / Q000502:physiology*; D013154:Spleen / Q000276:immunology*; D013744:Tetanus Toxin / Q000008:administration & dosage / Q000276:immunology*; D014836:Volition / Q000502:physiology
D000941:Antigens; D007074:Immunoglobulin G; D013744:Tetanus Toxin