Hydrolytically stable, diaminocarboxylic acid-based membranes buffering in the pH range from 6 to 8.5 for isoelectric trapping separations.
Diaminocarboxylic acid carrier ampholytes, such as L-histidine, 2,3-diaminopropionic acid, L-ornithine, and L-lysine, were reacted with glycerol-1,3-diglycidyl ether (GDGE) and poly(vinyl alcohol) (PVA) in the presence of sodium hydroxide to produce hydrolytically and mechanically stable hydrogels, supported on a PVA substrate, for use as buffering membranes in isoelectric trapping (IET) separations. The pH values of the DACAPVA membranes were determined with the help of small-molecule pI markers and proteins and were found to be in the 6 < pH < 8.5 range. The membranes were successfully used to isoelectrically trap small ampholytes, desalt ampholyte solutions in IET mode, and effect the binary separation of chicken egg white proteins.
Fleisher
Helen C
HC
Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Vigh
Gyula
G
D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000302:isolation & purification; D004852:Epoxy Compounds / Q000737:chemistry; D005995:Glyceryl Ethers / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D008567:Membranes, Artificial; D011142:Polyvinyl Alcohol / Q000737:chemistry
D004527:Egg Proteins; D004852:Epoxy Compounds; D005995:Glyceryl Ethers; D020100:Hydrogels; D008567:Membranes, Artificial; C058056:glycerol 1,3-diglycidyl ether; D011142:Polyvinyl Alcohol