Oligodendrocyte-myelin glycoprotein is present in lipid rafts and caveolin-1-enriched membranes.
The oligodendrocyte-myelin glycoprotein is a ligand of the neuronal Nogo receptor and a potent inhibitor of neurite outgrowth, but its physiological function remains to be elucidated. The oligodendrocyte-myelin glycoprotein is anchored solely in the outer leaflet of the plasma membrane via its glycosylphosphatidylinositol anchor, and through its leucine-rich repeat domain, it likely interacts with other proteins. In the present study, we compare its buoyancy and detergent solubility characteristics with those of other myelin proteins. Based on its detergent solubility profile and membrane fractionation using established ultracentrifugation procedures, we conclude that the oligodendrocyte-myelin glycoprotein is a lipid raft component that is closely associated with the axolemma. Moreover, it associates with caveolin-1 and caveolin-1-enriched membranes. We postulate that, by virtue of its concentration in lipid rafts and perhaps through interactions with caveolin-1, the oligodendrocyte-myelin glycoprotein may influence signaling pathways.
Boyanapalli
Madanamohan
M
Department of Neurology, University of Michigan, Ann Arbor, MI 48109-0322, USA.
Kottis
Vicky
V
Lahoud
Oscar
O
Bamri-Ezzine
Saoussen
S
Braun
Peter E
PE
Mikol
Daniel D
DD
D000818:Animals; D001369:Axons / Q000737:chemistry / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D058851:GPI-Linked Proteins; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000737:chemistry / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein / Q000737:chemistry / Q000378:metabolism*; D063308:Myelin-Oligodendrocyte Glycoprotein; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D012995:Solubility
D051242:Caveolin 1; D003902:Detergents; D058851:GPI-Linked Proteins; C571327:Mog protein, mouse; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; C560529:Omg protein, mouse