The effect of surface pore size on the differentiation of rat bone marrow cells: morphological observations and expression of bone related protein mRNA.
The purpose of this study was to investigate the behavior of rat bone marrow cells (RBM) growing on surfaces with different pore sizes. RBM behavior on Millipore filters (MF-Millipore membrane filter) made from cellulose mixed esters with 5 different pore surfaces (0.45 microm, 1.2 microm, 3.0 microm, 5.0 microm and 8.0 microm) were compared in terms of morphological changes on the different pore sizes. Furthermore, the expressions of osteopontin and osteocalcin mRNAs were investigated. On the 1.2 microm and 3.0 microm pore surfaces, RBM attached to the substrate well, but cells on the 5.0 microm and 8.0 microm pore surfaces invaded deeply into the pores. Higher levels of both osteopontin and osteocalcin mRNA expression were always observed in cells cultured on the 1.2 microm filter. These results suggest that the 1.2 microm Millipore filter pore size is the most suitable for inducing RBM to differentiate into an osteoblastic phenotype among these surfaces and is probably related to production of the ECM but not to the phenomenon of cell spreading.
Nashimoto
Masanori
M
Department of Pathology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
Matsuzaka
Kenichi
K
Yoshinari
Masao
M
Shimono
Masaki
M
Inoue
Takashi
T
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D048429:Cell Size; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000235:genetics; D008851:Micropore Filters / Q000145:classification*; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000032:analysis* / Q000235:genetics; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics; D016062:Porosity; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis* / Q000235:genetics
D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D015675:Osteocalcin; D053495:Osteopontin