title: Human papillomaviruses of the mucosal type are present in some cases of extragenital Bowen's disease.
abstract: BACKGROUND
  Bowen's disease in the genital area is generally considered to be caused by mucosal high-risk human papillomaviruses (HPVs). However, the detection rate and spectrum of HPVs in extragenital Bowen's disease are various and it is not clear to what extent HPV is involved in its pathogenesis.
  
  
  OBJECTIVE
  To assess the degree of association of HPV in extragenital cases by examining detection rates, types, quantities and localization of HPV.
  
  
  METHODS
  A polymerase chain reaction (PCR) approach that we had previously established, which can give sensitive detection of a broad range of HPVs from cutaneous [including epidermodysplasia verruciformis-related HPVs (EV-HPVs)] to mucosal HPVs, was applied to samples from 41 patients with extragenital Bowen's disease and normal skin samples from 48 individuals. Semiquantitative L1-PCR and tyramide-based in situ hybridization (ISH) were also employed for positive cases.
  
  
  RESULTS
  HPVs belonging to the mucosal high-risk group were detected in three patients with Bowen's disease (7%; two HPV 16 and one HPV 33), with 10(1)-10(3) copy equivalents per diploid amount of cellular DNA. They were distributed among most nuclei of tumour cells but in none of the cells of adjacent normal skin. HPVs belonging to the cutaneous group were detected in two patients (5%; HPV 27 and HPV 76) at 10(-2)-10(-3) copy equivalents, the same level as in a normal skin specimen positive for type 23 EV-HPV. No positive signals were observed by ISH.
  
  
  CONCLUSIONS
  HPVs belonging to the mucosal high-risk group may participate in the development of extragenital Bowen's disease.
authors.affiliation: Department of Dermatology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.forename: A
authors.initials: A
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: S-I
authors.initials: SI
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Yasue
authors.forename: A
authors.initials: A
authors.lastname: Sakakibara
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: T
authors.initials: T
authors.lastname: Nagasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Tomita
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001913:Bowen's Disease / Q000821:virology*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000821:virology; D012878:Skin Neoplasms / Q000821:virology*; D014412:Tumor Virus Infections / Q000821:virology*
chemical_list: D004279:DNA, Viral