Microplate ELISA for urine microalbumin: reference values and results in patients with type 2 diabetes and cardiovascular disease.
An ELISA for urine microalbumin using microtiter plates has been developed. The assay uses polyclonal anti-human albumin antibody for coating the microtiter plates and the same antibody conjugated with horseradish peroxidase for detection. The assay sensitivity is 1.6 microg/ml. Results by this in-house ELISA show good correlation (r = 0.99) with those obtained by a commercial assay using the Behring BNII autoanalyzer. Within-day and between-day CVs are 10%. Reference values for microalbumin in 769 urine specimens from healthy Chinese subjects were higher in women than men and higher in subjects 50 yr than in those <50 yr of age. Elevated mean concentrations of urine microalbumin were observed in patients with type 2 diabetes and CVD. This in-house ELISA is simple, sensitive, precise, and especially suited for laboratories without expensive autoanalyzers.
Wu
Tsu-Lan
TL
Department of Pathology, Chang Gung Memorial Hospital, Taipei, Taiwan.
Chang
Pi-yueh
PY
Li
Chia-Chi
CC
Tsao
Kuo-Chien
KC
Sun
Chien-Feng
CF
Wu
James T
JT
D000418:Albumins / Q000032:analysis*; D000419:Albuminuria / Q000175:diagnosis; D002318:Cardiovascular Diseases / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D016482:Urinalysis / Q000379:methods
D000418:Albumins