title: [Role of tissue factor in the invasion of cultured human colon carcinoma cells and its correlation with matrix metalloproteinases].
abstract: OBJECTIVE
  To investigate the role of tissue factor expression in the invasive ability of human colon carcinoma cells and to analyze the correlation between tissue factor and MMP-2 and MMP-9.
  
  
  METHODS
  HT-29 cells with sense-TFcDNA transfection and LoVo cells with antisense-TFcDNA transfection were implanted subcutaneously into nude mice as well as controls. The expressions of MMP-9 and MMP-2 at the protein and mRNA levels were detected by Western blot and RT-PCR respectively; Gelatin zymography was used for assay of the MMP-9 and MMP-2 activities in the supernatant cultured media of LoVo cells with antisense-TFcDNA transfection and controls.
  
  
  RESULTS
  The expressions of MMP-9 and MMP-2 at the protein and mRNA levels in the group of HT-29 cells with sense-TFcDNA were significantly increased with untransfected HT-29 cells. The expression in the group of LoVo cells with antisense-TFcDNA had a significant decrease than that of the controls. The MMP-9 and MMP-2 activities of LoVo cells with antisense-TFcDNA were weakened because of the lower expression of tissue factor by gelatin zymography. The expression of MMP-9 and MMP-2 were closely correlated to the expression of tissue factor.
  
  
  CONCLUSIONS
  Tissue factor can increase the invasive ability of human colon carcinoma cells. The partial mechanism is that TF can upregulate the expressions of MMP-9 and MMP-2. Tissue factor may be an inner agonist in the secretion of MMPs in colon carcinoma cells.
authors.affiliation: Department of General Surgery, Peking University First Hospital, Beijing 100034, China.
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Tang
authors.forename: Yuan-lian
authors.initials: YL
authors.lastname: Wan
authors.forename: Yu-cun
authors.initials: YC
authors.lastname: Liu
authors.forename: Long
authors.initials: L
authors.lastname: Rong
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Yi-sheng
authors.initials: YS
authors.lastname: Pan
authors.forename: Jing-ming
authors.initials: JM
authors.lastname: Ye
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D013925:Thromboplastin; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9