Cyclin-dependent kinase 11(p58) interacts with HBO1 and enhances its histone acetyltransferase activity.
CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. Here we report that histone acetyltransferase (HAT) HBO1, a MYST family protein, interacts with CDK11(p58) in vitro and in vivo. CDK11(p58) and HBO1 colocalize in the cell nucleus. Recombinant CDK11(p58) enhances the HAT activity of HBO1 significantly in vitro. Meanwhile, overexpression of CDK11(p58) in mammalian cells leads to the enhanced HAT activity of HBO1 towards free histones. Thus, we conclude that CDK11(p58) is a new interacting protein and a novel regulator of HBO1. Both of the proteins may be involved in the regulation of eukaryotic transcription.
Zong
Hongliang
H
State Key Laboratory of Genetic Engineering & Gene Research Center, Shanghai Medical College of Fudan University, P.O. Box 103, No. 138 Yi Xue Yuan Road, 200032 Shanghai, People's Republic of China.
Li
Zejuan
Z
Liu
Liyun
L
Hong
Yi
Y
Yun
Xiaojing
X
Jiang
Jianhai
J
Chi
Yayun
Y
Wang
Hanzhou
H
Shen
Xiaoyun
X
Hu
Yun
Y
Niu
Ziyue
Z
Gu
Jianxin
J
D000123:Acetyltransferases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry; D018844:Cyclin-Dependent Kinases; D015723:Gene Library; D051548:Histone Acetyltransferases; D006801:Humans; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D017384:Sequence Deletion; D014158:Transcription, Genetic / Q000502:physiology; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques
D000123:Acetyltransferases; D051548:Histone Acetyltransferases; C496553:KAT7 protein, human; C496552:KAT8 protein, human; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases