Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples.
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
Marques
Fabiana Rangel
FR
Dermatological, Infectious and Parasitical Diseases Department, Faculty of Medicine, Center for Microorganism Investigation, São José do Rio Preto, SP, Brazil.
Cardoso
Luciana Ventura
LV
Cavasini
Carlos Eugênio
CE
Almeida
Magali Carmem de
MC
Bassi
Nair Aparecida
NA
Almeida
Margarete Teresa Gottardo de
MT
Rossit
Andréa Regina Baptista
AR
Machado
Ricardo Luiz Dantas
RL
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000469:parasitology*; D000818:Animals; D016022:Case-Control Studies; D003457:Cryptosporidiosis / Q000175:diagnosis*; D003458:Cryptosporidium / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity