[Characterization of the interaction between low density lipoprotein receptor-related protein-associated protein 1 and the cancer and embryo expression protein 65].
OBJECTIVE
To understand the mechanism of cancer and embryo expression protein 65 (CEP65) in cancer development.


METHODS
CEP65 was used as a bait protein to isolate the partners of CEP65 from a human placenta cDNA library with yeast two hybrid system. The DNA fragments from positive clone were expressed prokaryotic and mammalian cells respectively, the GST pull-down experiment was performed to ascertain the binding activity.


RESULTS
After screening a human placenta cDNA library, the low density lipoprotein receptor-related protein-associated protein 1 (RAP) was isolated and shown to interact with CEP65. GST pull-down assay results indicated that His-CEP65 and GST-RAP expressed by prokaryotic system were immunoprecipitated, and so were Myc-RAP and GST-P65 expressed by mammalian cells.


CONCLUSIONS
RAP can interact with CEP65 and RAP may be a candidate to mediate the function of CEP65.
Jin
Geng-lin
GL
Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100034, China.
Zhang
Jian-zhi
JZ
Su
Rong
R
Liu
Xiao-ying
XY
Wu
Jian
J
Meng
Lin
L
Shou
Cheng-chao
CC
D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D019556:COS Cells; D018797:Cell Cycle Proteins; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques
D000951:Antigens, Neoplasm; D018797:Cell Cycle Proteins; C493903:Cep290 protein, human; D003598:Cytoskeletal Proteins; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins