Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus).
Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N2 min(-1) after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus. The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
Linke
Diana
D
Universität Hannover, Institut für Lebensmittelchemie, D-30453 Hannover, Germany.
Zorn
Holger
H
Gerken
Birte
B
Parlar
Harun
H
Berger
Ralf G
RG
D005110:Extracellular Space / Q000201:enzymology; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology*
D005656:Fungal Proteins; D008049:Lipase