title: A high-throughput structural biology/proteomics beamline at the SRS on a new multipole wiggler.
abstract: The North West Structural Genomics Centre's beamline, MAD10, at the SRS receives the central part of the radiation fan (0.5 mrad vertically, 4 mrad horizontally) produced by a new 2.46 T ten-pole wiggler. The optical arrangement of the beamline consists of a Rh-coated collimating Si mirror, a fixed-exit-beam double-crystal monochromator with sagittal bending for horizontal focusing and a second Rh-coated Si mirror for vertical focusing. The double-crystal Si (111) monochromator allows data collection in the 5-13.5 keV photon energy range with rapid (subsecond) tunability and high energy resolution. The monochromatic beam is optimized through a 200 microm collimator. The beamline end station has been designed around a Mar desktop beamline with high-throughput cryogenic sample changer, Mar225 CCD detector, liquid-N(2) autofill system and an ORTEC C-TRAIN-04 energy-resolving high-count-rate X-ray fluorescence detector. The instrument is optimized for MAD/SAD applications in protein crystallography with the additional mode of operation of online single-crystal EXAFS studies on the same crystals. Thus, screening of metals/Se in the crystal can be performed quickly prior to MAD/SAD data collection by exciting the crystal with X-rays of appropriate energy and recording an energy-dispersive fluorescence spectrum. In addition, this experimental set-up allows for parallel XAFS measurements on the same crystal to monitor 'radiation-induced' changes, if any, in e.g. the redox state of metal centres to be detected for a 'metallic' functional group during crystallographic data collection. Moreover, careful minimization of the thickness of the Be window maximizes the intensity performance for the 2.0-2.5 A softer wavelength range. This range also covers the K-edges of a number of important 3d transition metals as well as the L-edges of xenon and iodine and enhanced sulfur f ''.
authors.affiliation: CCLRC Daresbury Laboratory, Warrington WA4 4AD, Cheshire, UK.
authors.forename: Michele
authors.initials: M
authors.lastname: Cianci
authors.forename: Svetlana
authors.initials: S
authors.lastname: Antonyuk
authors.forename: Neil
authors.initials: N
authors.lastname: Bliss
authors.forename: Mike W
authors.initials: MW
authors.lastname: Bailey
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Buffey
authors.forename: Kan C
authors.initials: KC
authors.lastname: Cheung
authors.forename: Jim A
authors.initials: JA
authors.lastname: Clarke
authors.forename: Gareth E
authors.initials: GE
authors.lastname: Derbyshire
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Ellis
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Enderby
authors.forename: Alan F
authors.initials: AF
authors.lastname: Grant
authors.forename: Martin P
authors.initials: MP
authors.lastname: Holbourn
authors.forename: David
authors.initials: D
authors.lastname: Laundy
authors.forename: Colin
authors.initials: C
authors.lastname: Nave
authors.forename: Roy
authors.initials: R
authors.lastname: Ryder
authors.forename: Paul
authors.initials: P
authors.lastname: Stephenson
authors.forename: John R
authors.initials: JR
authors.lastname: Helliwell
authors.forename: S Samar
authors.initials: SS
authors.lastname: Hasnain
mesh_terms: D000465:Algorithms; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008967:Molecular Biology / Q000295:instrumentation* / Q000379:methods; D011487:Protein Conformation; D020543:Proteome / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods; D013052:Spectrometry, X-Ray Emission / Q000295:instrumentation* / Q000379:methods; D013482:Superoxide Dismutase / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D014584:User-Computer Interface
chemical_list: D020543:Proteome; D013482:Superoxide Dismutase