Influence of extender, freezing rate, and thawing rate on post-thaw motility, viability and morphology of coyote (Canis latrans) spermatozoa.
The objective of this study was to examine the post-thaw effects of three cryoprotective extenders (Tris-fructose-citric acid extender, Tris-glucose-citric acid extender, and lactose extender), three linear freezing rates (-1, -6, and -20 degrees C/min), and three thawing rates (37 degrees C water bath for 120s, 60 degrees C water bath for 30s, and 70 degrees C water bath for 8s) on coyote spermatozoa. After thawing, the findings supported that cryopreservation of coyote (Canis latrans) spermatozoa frozen at a moderate freezing rate (-6 degrees C/min), in either a Tris-fructose or Tris-glucose extender, and thawed at a slow rate (37 degrees C water bath for 120s) or moderate rate (60 degrees C water bath for 30s), resulted in a more vigorous post-thaw motility (range, 57.5-44.0%) and viability (range, 64-49.6%) with the least amount of morphological and acrosomal abnormalities.
Minter
Larry J
LJ
Utah State University, Department of Fisheries and Wildlife, Logan, UT 84322, USA. ljminter@yahoo.com
Deliberto
Thomas J
TJ
D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002470:Cell Survival; D019343:Citric Acid / Q000008:administration & dosage; D046029:Coyotes; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005632:Fructose / Q000008:administration & dosage; D005947:Glucose / Q000008:administration & dosage; D006358:Hot Temperature; D007785:Lactose / Q000008:administration & dosage; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities* / Q000502:physiology; D013997:Time Factors; D014325:Tromethamine / Q000008:administration & dosage
D003451:Cryoprotective Agents; D014325:Tromethamine; D019343:Citric Acid; D005632:Fructose; D005947:Glucose; D007785:Lactose