Spectral-domain phase microscopy.
Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.
Choma
Michael A
MA
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. mac32@duke.edu
Ellerbee
Audrey K
AK
Yang
Changhuei
C
Creazzo
Tony L
TL
Izatt
Joseph A
JA
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods