Bcl6 regulates Th2 type cytokine productions by mast cells activated by FcepsilonRI/IgE cross-linking.
Bcl6-deficient (Bcl6-/-) mice displayed Th2 type inflammation, which caused by abnormality of non-lymphoid cells. However, initiators for the Th2 type inflammation were not clear. In order to elucidate the initiators, we investigated property and function of mast cells derived from Bcl6-/- mice. Mast cells were developed from bone marrow cells cultured with IL-3 (BMMCs). Although the development of BMMCs from Bcl6-/- mice was similar to that from wild-type mice, proliferation of Bcl6-/- BMMCs stimulated with IL-3 was slightly lower than that of wild-type BMMCs. When these BMMCs were stimulated by FcepsilonRI/IgE cross-linking, Bcl6-/- BMMCs produced Th2 cytokines more than wild-type BMMCs did. Thus, Bcl6-/- mast cells are one of the initiators for Th2 type inflammation in Bcl6-/- mice, and Bcl6 may be a molecular target for Th2 type allergic diseases.
Ohtsuka
Yuichiro
Y
Department of Developmental Genetics H2, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
Arima
Masafumi
M
Fujimura
Lisa
L
Li
Haifeng
H
Sakamoto
Akemi
A
Okamoto
Yoshitaka
Y
Tokuhisa
Takeshi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000276:immunology; D049109:Cell Proliferation; D002470:Cell Survival; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000276:immunology; D004140:Dinitrophenols / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051560:Proto-Oncogene Proteins c-bcl-6; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000276:immunology*; D012709:Serum Albumin / Q000494:pharmacology; D018418:Th2 Cells / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000911:Antibodies, Monoclonal; C490010:Bcl6 protein, mouse; D016207:Cytokines; D004268:DNA-Binding Proteins; D004140:Dinitrophenols; D051560:Proto-Oncogene Proteins c-bcl-6; D012333:RNA, Messenger; D017455:Receptors, IgE; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D007073:Immunoglobulin E; D019009:Proto-Oncogene Proteins c-kit; D001619:beta-N-Acetylhexosaminidases