title: Expression of matrix metalloproteinase 7, laminin and type IV collagen-associated liver metastasis in human colorectal cancer: immunohistochemical approach.
abstract: OBJECTIVE
  Matrix metalloproteinase 7 (MMP-7) plays an important role in vessel invasion and metastasis in human colorectal cancer.
  
  
  METHODS
  The significance of MMP-7, laminin and type IV collagen expression in human colorectal cancer was investigated by immunohistochemical assay, and the correlation with liver metastasis was analyzed.
  
  
  RESULTS
  In a synchronous metastasis group, 26 of 36 cases (72%) showed positive staining of MMP-7: There were 32 cases (89%) in the lymph channel and 28/32 cases (87%) in the vessels, and 17/34 cases (50%) showed a positive rate of laminin. In the metachronous metastasis group, 14 of 30 cases (47%) showed positive staining of MMP-7: There were 19 cases (63%) in the lymph channel and 13/19 cases (69%) in the vessels, and 17/30 cases (57%) showed a positive rate of laminin. In the control group, which was a 5-year disease-free group, despite there being no significant clinicopathological factors compared with the other two groups, 17 of 37 cases (51%) showed positive staining of MMP-7: There were 12 cases (37%) in the lymph channel and 6 cases (18%) in the vessels, and 2/31 cases (5%) showed a positive rate of laminin. The expression of type IV collagen attenuated in 19 out of 32 cases (59%) in Group S, 10 out of 19 cases (53%) in Group M, and 14 out of 37 cases (38%) in Group C, with no significant differences among the groups. Thus, the metastatic groups were significantly higher than the control group in terms of expression of laminin and MMP-7 in the lymph channel.
  
  
  CONCLUSIONS
  These findings suggest that laminin and the expression of MMP-7 in the lymph channel is a useful parameter for predicting liver metastasis.
authors.affiliation: Department of Surgery 1, Jikei University School of Medicine, Tokyo, Japan. masatchmo@k5.dion.ne.jp
authors.forename: Masaichi
authors.initials: M
authors.lastname: Ogawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Ikeuchi
authors.forename: Michiaki
authors.initials: M
authors.lastname: Watanabe
authors.forename: Ken
authors.initials: K
authors.lastname: Etoh
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yoshihiiko
authors.initials: Y
authors.lastname: Takao
authors.forename: Sadao
authors.initials: S
authors.lastname: Anazawa
authors.forename: Yoji
authors.initials: Y
authors.lastname: Yamazaki
mesh_terms: D000368:Aged; D024141:Collagen Type IV / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis*; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D020783:Matrix Metalloproteinase 7; D008666:Metalloendopeptidases / Q000032:analysis*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D009378:Neoplasms, Multiple Primary / Q000473:pathology; D016609:Neoplasms, Second Primary / Q000473:pathology; D011379:Prognosis; D012016:Reference Values; D013223:Statistics as Topic
chemical_list: D024141:Collagen Type IV; D007797:Laminin; D008666:Metalloendopeptidases; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7