title: New cytotoxic cyclobutanoid amides, a new furanoid lignan and anti-platelet aggregation constituents from Piper arborescens.
abstract: Three new cyclobutanoid amides with trans-trans-trans configurations, piperarborenine C, piperarborenine D and piperarborenine E, and a new furanoid lignan, (+)-arborone, together with twelve known compounds, were isolated from the stems of Piper arborescens. The structures of these new compounds were determined by means of spectral analyses. Piperarborenine C, (+)-diayangambin, piplartine, piperolactam B, piperolactam C, aristolactam BIII, goniothalactam, and methyl trans-3,4,5-trimethoxycinnamate possessed anti-platelet aggregation activity in vitro. Among them, piplartine showed the most potent anti-platelet aggregation activity induced by collagen and showed an IC50 value of 21.5 microM. Piperarborenines A - E, piperarborenine, aristololactam BIII and goniothalactam showed significant cytotoxic activity (IC50 values < 4 microg/mL) against P-388, HT-29 and A549 cell lines in vitro.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
authors.forename: Ian-Lih
authors.initials: IL
authors.lastname: Tsai
authors.forename: Fan-Pin
authors.initials: FP
authors.lastname: Lee
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Chang-Yih
authors.initials: CY
authors.lastname: Duh
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Jih-Jung
authors.initials: JJ
authors.lastname: Chen
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Hiroko
authors.initials: H
authors.lastname: Seki
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008517:Phytotherapy; D031701:Piper; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors