[Effect of curcumin on expression of human low density lipoprotein receptors in Xenopus Laevis oocytes].
OBJECTIVE
To investigate the molecular mechanism of curcumin in reducing blood lipids by establishing gene expression system of human low density lipoprotein receptors (LDL-R) in Xenopus Laevis oocytes (XLO).


METHODS
The expression of LDL-R on cytomembrane was determined using immuno-fluorescent, ligand-fluorescent and immune colloidal gold techniques after human LDL-R containing p3.7 LDL plasmid was led into nucleus. And the expression of LDL-R gene in XLO was quantitatively determined by ELISA after being interfered with different concentrations of curcumin.


RESULTS
The human LDL-R gene could be expressed on XLO, which could be significantly enhanced by curcumin in a dose-dependent manner. Conclusion One of the paths of curcumin in reducing blood lipids and anti-atherosclerosis was improving LDL-R gene expression and increasing the LDL-cholesterol absorption of cells.
Fan
Chun-lei
CL
Department of Life Science, Zhejiang College of Traditional Chinese Medicine, Hangzhou.
Wo
Xing-de
XD
Luo
Yan
Y
D000818:Animals; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics; D014982:Xenopus laevis
D011973:Receptors, LDL; D003474:Curcumin