title: Inhibitory effect of Gamibojungikgitang extract on mast cell-mediated allergic reaction in murine model.
abstract: OBJECTIVE
  Gamibojungikgitang (GBIT) is an Oriental herbal prescription medication, which has been commonly used to treat allergic rhinitis in far Eastern countries including Korea, China, and Japan. Additionally, GBIT effectively treats ovarian cysts and improves ovarian functions by regulating both endocrine and metabolic activities. However, it has not been cleared how it prevents allergic diseases in experimental model. Here we report the effect of GBIT on mast cell-mediated allergic reactions.
  
  
  METHODS
  The anti-anaphylactic effect of GBIT extract was studied against compound 48/80-induced systemic anaphylactic shock model in mice. Rat Peritoneal Mast Cells (RPMCs) were used to investigate the effect of GBIT extract on histamine release induced by compound 48/80. Passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE was used to know the effect of GBIT extract. In addition, human mast cell line HMC-1 cells culture supernatants that GBIT extract pretreated were assayed for IL-6 protein levels by enzyme-linked immunosorbent assay method.
  
  
  RESULTS
  GBIT extract dose dependently inhibited compound 48/80-induced systemic anaphylactic shock. When GBIT extract was given as pretreatment at concentrations ranging 0.01-1 mg/ml, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. GBIT extract also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE. In addition, GBIT extract inhibited phorbol 12-myristate 13-acetate + A23187-induced interleukin-6 secretion from human mast cell line HMC-1 cells.
  
  
  CONCLUSIONS
  These results suggest a potential role for GBIT extract as a source of anti-anaphylactic agents for allergic disorders.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, South Korea.
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Jo-Jeong
authors.initials: JJ
authors.lastname: Na
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Chae
authors.forename: Hyung-Ryong
authors.initials: HR
authors.lastname: Kim
authors.forename: Jeong-On
authors.initials: JO
authors.lastname: Choi
authors.forename: Si-Hyeong
authors.initials: SH
authors.lastname: Lee
authors.forename: Jo-Young
authors.initials: JY
authors.lastname: Shin
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018514:Plant Structures; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal