[Effect of the pharmacological agent hesperadin on breast and prostate tumor cultured cells].
Aurora B, which is important for cell division control, is highly expressed in large number of cancer cell lines. Hesperadin, a prototype of a pharmacological agent, is a small molecule inhibitor of catalytic activity of Aurora B. In present work we investigate effect of Hesperadin on breast--MCF7 and prostate adenocarcinoma--PC3, cancer cell lines. After Hesperadin treatment we observe stop of cell proliferation due to appearance of multiple mitotic defects caused by Aurora B activity reduction and elimination of checkpoint proteins--such as hBUBR1 and CENP-E--from kinetochores of mitotic chromosomes.
Ladygina
N G
NG
Latsis
R V
RV
Yen
T
T
D064107:Aurora Kinase B; D064127:Aurora Kinases; D001943:Breast Neoplasms; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002868:Chromosomal Proteins, Non-Histone / Q000037:antagonists & inhibitors; D005260:Female; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D018386:Kinetochores / Q000378:metabolism; D008297:Male; D011471:Prostatic Neoplasms; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology*
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D007211:Indoles; D013449:Sulfonamides; C068688:centromere protein E; D011494:Protein Kinases; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases; C474723:hesperadin