Detecting mismatch repair defects in myeloma.
Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma.
Velangi
Mark
M
Northern Institute for Cancer Research, ?Medical School, Newcastle upon Tyne, UK.
Matheson
Elizabeth
E
Hall
Andrew
A
Irving
Julie
J
D015703:Antigens, CD / Q000235:genetics; D020137:Base Pair Mismatch / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D018895:Microsatellite Repeats; D009101:Multiple Myeloma / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D011509:Proteoglycans / Q000235:genetics; D053668:Syndecan-1; D053667:Syndecans
D015703:Antigens, CD; D017931:DNA Primers; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans