title: The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron.
abstract: Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD ( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.
authors.affiliation: National Institute for Cellular Biotechnology, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Reiber
authors.forename: Emer P
authors.initials: EP
authors.lastname: Reeves
authors.forename: Claire M
authors.initials: CM
authors.lastname: Neville
authors.forename: Robert
authors.initials: R
authors.lastname: Winkler
authors.forename: Peter
authors.initials: P
authors.lastname: Gebhardt
authors.forename: Kevin
authors.initials: K
authors.lastname: Kavanagh
authors.forename: Sean
authors.initials: S
authors.lastname: Doyle
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017262:Siderophores / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017262:Siderophores; D007501:Iron; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase