DNA damages in ovarian surface epithelial cells of ovulatory hens.
A cause-effect relationship between ovulation and common (surface) epithelial ovarian cancer has been suspected for many years. The ovarian surface epithelium apparently becomes exposed to genotoxins that are generated during the ovulatory process. Intensive egg-laying hens readily develop ovarian carcinomatosis. Indeed, elevated levels of potentially mutagenic 8-oxo-guanine adducts were detected in avian ovarian epithelial cells isolated from the apical surfaces and perimeters of pre-and postovulatory follicles, respectively. Internucleosomal DNA fragmentation indicative of apoptosis was evident in ovarian surface epithelial cells associated with the formative site of ovulation (stigma line) and regressive ruptured follicles. It is conceivable that a genetically altered progenitor cell with unrepaired DNA but not committed to death (i.e., a unifocal "escape") could give rise to a transformed phenotype. Hence, the high rate of ovarian cancer in egg-laying hens could be the consequence of genomic damages to the ovarian surface epithelium associated with incessant ovulations, thereby increasing the likelihood of mutation and clonal expansion.
Murdoch
William J
WJ
Department of Animal Science and Reproductive Biology Program, 1000 E. University Avenue, University of Wyoming, Laramie, Wyoming 82071, USA. wmurdoch@uwyo.edu
Van Kirk
Edward A
EA
Alexander
Brenda M
BM
D000818:Animals; D017209:Apoptosis; D002645:Chickens; D018736:DNA Adducts / Q000096:biosynthesis; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005498:Follicular Phase; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008183:Luteal Phase; D006080:Ovarian Follicle / Q000378:metabolism / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000209:etiology*; D010060:Ovulation
D018736:DNA Adducts; C024829:8-hydroxyguanine; D006147:Guanine