title: Enteropathogenic Escherichia coli strains carrying genes encoding the PER-2 and TEM-116 extended-spectrum beta-lactamases isolated from children with diarrhea in Uruguay.
abstract: We studied 13 extended-spectrum beta-lactamase (ESBL)-producing enteropathogenic Escherichia coli isolates from children suffering acute diarrhea in Uruguay. ESBL characterization in crude extracts showed a single band at pI 5.4. PCR amplification and sequencing data allowed identification of blaPER-2 and blaTEM-116. Retrospective analysis suggests that these strains were disseminated in the community, even if unnoticed, prior to their access to the hospital environment more than a decade ago.
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, 11600 Montevideo, Uruguay.
authors.forename: Rafael
authors.initials: R
authors.lastname: Vignoli
authors.forename: Gustavo
authors.initials: G
authors.lastname: Varela
authors.forename: María Inés
authors.initials: MI
authors.lastname: Mota
authors.forename: Nicolas F
authors.initials: NF
authors.lastname: Cordeiro
authors.forename: Pablo
authors.initials: P
authors.lastname: Power
authors.forename: Elizabet
authors.initials: E
authors.lastname: Ingold
authors.forename: Pilar
authors.initials: P
authors.lastname: Gadea
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sirok
authors.forename: Felipe
authors.initials: F
authors.lastname: Schelotto
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ayala
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutkind
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018550:Cephalosporin Resistance; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014583:Uruguay / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C472387:beta-lactamase TEM-103, E coli