Study of alanine-73 and aspartate-9 of HLA-C locus in Saudi psoriasis patients, using sequence-specific primers (PCR-SSP).
Alanine at residue 73 (Ala-73) and aspartate at residue 9 (Asp-9) are characteristic to both Cw6 and Cw7 alleles of HLA-C gene and have been suggested as possible markers for psoriasis vulgaris (PsV). However, the results from various ethnic groups/populations are contradictory and inconclusive. In this study, an attempt has been made to examine the association between HLA-C (Ala-73 and Asp-9) and susceptibility to PsV among Saudi patients. Genomic DNA was extracted from 25 Saudi PsV patients and 75 control subjects. Polymerase chain reaction (PCR) was performed to amplify HLA-C sequences using earlier reported primers, C133P and C243PR. Sequence-specific primers were used to specifically detect nucleotide coding for Ala-73 and Asp-9 in all the subjects. The results showed significantly higher frequency of Asp-9 (84.0 % versus 61.3 %) in PsV patients as compared to controls (p < 0.05, 2-tailed Fisher's exact test). The frequencies of Ala-73 among PsV patients (92 %) and controls (88 %) did not differ significantly.
Abanmi
Abdullah
A
Department of Dermatology, Armed Forces Hospital, Riyadh, Saudi Arabia.
Al Harthi
Fahad
F
Al Agla
Rokaiyah
R
Khan
Haseeb Ahmad
HA
Tariq
Mohammad
M
D000409:Alanine; D001224:Aspartic Acid; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D015236:HLA-C Antigens / Q000737:chemistry*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011565:Psoriasis / Q000235:genetics* / Q000276:immunology*; D012016:Reference Values; D012529:Saudi Arabia
D017931:DNA Primers; D015236:HLA-C Antigens; D001224:Aspartic Acid; D004247:DNA; D000409:Alanine