title: High expressions of caveolins on the proliferating bile ductules in primary biliary cirrhosis.
abstract: OBJECTIVE
  An increase in bile ductular structures is observed in diverse human liver diseases, especially in primary biliary cirrhosis (PBC). These structures harbor the progenitor cell component of the liver. Caveolins are cholesterol-binding proteins involved in the regulation of several intracellular processes including cholesterol transport. This study aims to examine the role of caveolin in PBC.
  
  
  METHODS
  Immunohistochemical and Western blotting studies were performed on human liver specimens obtained from patients with PBC and normal liver samples. The expression of caveolin (CAV)-1 and -2 was determined using specific antibodies.
  
  
  RESULTS
  In normal liver, scanty immunostaining for CAV-1 and -2 was observed in bile ductules. In PBC liver samples, the expression levels of CAV-1 and -2 were increased on proliferating bile ductules especially in stage 3 cases, but was sparse on interlobular bile duct in stage 1 specimens. Especially, the regenerating bile ductules at the interface of portal tracts and necrotic areas were immunostained intensely for CAV-1 and -2. These phenomena were confirmed by Western blot.
  
  
  CONCLUSIONS
  The present results demonstrate increased expression of caveolins in proliferating bile ductules in PBC, which may be related to the homeostasis of cholesterol transport in regenerating bile ductules in PBC liver.
authors.affiliation: Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Kitamotoshi, Saitama 364-8501, Japan. yokomori-hr@kitasato.or.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yokomori
authors.forename: Masaya
authors.initials: M
authors.lastname: Oda
authors.forename: Go
authors.initials: G
authors.lastname: Wakabayashi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nomura
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
mesh_terms: D000328:Adult; D000368:Aged; D001652:Bile Ducts / Q000378:metabolism / Q000473:pathology*; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D005260:Female; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins