title: [Biological effects of nano-hydroxyapatite/polyamide 66 on the osteoblast].
abstract: OBJECTIVE
  To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.
  
  
  METHODS
  MTT assay was used to determine the growth of osteoblast, enzymatic measure was used to determine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in osteoblasts treated by DMEM eluate of nHA-PA66.
  
  
  RESULTS
  Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98% - 106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P > 0.05).
  
  
  CONCLUSIONS
  nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compatibility is proved.
authors.affiliation: Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.forename: Qin
authors.initials: Q
authors.lastname: Su
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D017886:Durapatite / Q000494:pharmacology*; D009757:Nylons / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D009757:Nylons; D012333:RNA, Messenger; D015675:Osteocalcin; D017886:Durapatite