title: The role of XTRAP-gamma in Xenopus pronephros development.
abstract: We isolated and characterized the Xenopus translocon-associated protein XTRAP-gamma, one of four subunits of the translocon-associated protein complex. TRAP has been proposed to aid the translocation of nascent polypeptides into the lumen of the endoplasmic reticulum, but this has not been demonstrated until now. XTRAP-gamma was specifically expressed in the pronephros tubules of Xenopus laevis from stage 25 during kidney development. Antisense morpholino oligonucleotide-mediated knockdown of XTRAP-gamma suppressed tubulogenesis and decreased expression of the pronephros marker genes Pax-2 and Wnt-4. XTRAP-gamma morpholinos also inhibited differentiation of the pronephros in activin/retinoic acid-treated animal caps. We conclude that XTRAP-gamma plays an important role in the process of pronephros differentiation.
authors.affiliation: Department of Pediatric Surgery and Oncology, Graduate School of Medicine, The University of Tokyo, Japan.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Li
authors.forename: Techuan
authors.initials: T
authors.lastname: Chan
authors.forename: Reiko
authors.initials: R
authors.lastname: Satow
authors.forename: Shinji
authors.initials: S
authors.lastname: Komazaki
authors.forename: Kouhei
authors.initials: K
authors.lastname: Hashizume
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D028341:Activins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D007265:Inhibins; D007668:Kidney / Q000196:embryology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018000:Receptors, Peptide / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D002135:Calcium-Binding Proteins; D018398:Homeodomain Proteins; D008562:Membrane Glycoproteins; D016376:Oligonucleotides, Antisense; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D029867:Xenopus Proteins; C066973:signal sequence receptor; D028341:Activins; D007265:Inhibins