[Transient expression of GUS gene controlled by different regulator sequences in rice and Populus tomrutosa].
GUS gene controlled by several kinds of regulator sequences (promoters, TMVOmega enhancer and fragments of 18S rRNA gene from Arabidopsis thaliane) were introduced into the calli of rice and Populus tomrutosa respectively by gene gun. The transient expression of GUS gene in the calli of rice and P. tomrutosa was investigated. The results showed: (1) in the calli of rice, Ubil promoter was the most effective one among the several regulator sequences and CaMV35S with fragments of 18S rRNA gene is the least effective one; (2) in the calli of P. tomrutosa, CaMV35S promoter with 18S rDNA fragments was the most effective one among the several regulator sequences; and (3) tandem CaMV35S-Ubil promoter with TMVOmega sequence could not enhance significantly the expression of GUS gene in the calli of rice and P. tomrutosa.
Liu
Gen-Qi
GQ
Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China. genqiliu@sina.com.cn
Li
Xiu-Li
XL
Yang
Chun-Ling
CL
Chen
Zhong
Z
Zhao
Shi-Min
SM
D019470:Biolistics / Q000379:methods; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D032107:Populus / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
D005966:Glucuronidase