title: [Leukemic bone marrow stromal cells in vitro protect leukemic cell line Jurkat cells from daunorubicin-induced apoptosis].
abstract: OBJECTIVE
  Tumor microenvironment affects tumor cells growth. Bone marrow microenvironment may protect leukemic cells from drug-induced damages, but the mechanism is unclear. This study was to explore the protection of bone marrow stromal cells (BMSCs) on leukemic cells against chemotherapy and its mechanism.
  
  
  METHODS
  Normal and leukemic BMSCs were isolated using Percoll, and cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. After treatment of 0.5 micromol/L of daunorubicin (DNR), apoptosis and cell cycle distribution of Jurkat cells were analyzed by flow cytometry.
  
  
  RESULTS
  When treated with DNR for 24 h, apoptosis rate of normal BMSCs-cocultured Jurkat cells was significantly lower than that of Jurkat cells without coculture [(8.39+/-4.08)% vs. (16.02+/-1.00)%, P < 0.05], and apoptosis rate of leukemic BMSCs-cocultured Jurkat cells was significantly lower than that of normal BMSCs-cocultured Jurkat cells [(5.73+/-1.78)% vs. (8.39+/-4.08)%, P < 0.05]; G(0)/G(1) phase percentage of BMSCs-cocultured Jurkat cells was significantly higher than that of Jurkat cells without coculture (P < 0.05), but the difference between Jurkat cells cocultured with normal and leukemic BMSCs was not significant (P > 0.05).
  
  
  CONCLUSIONS
  Leukemic BMSCs may inhibit DNR-induced apoptosis in leukemic cells partly through G(0)/G(1) phase arrest.
authors.affiliation: Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P. R. China.
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Ben-Xiu
authors.initials: BX
authors.lastname: Teng
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Pei-Yan
authors.initials: PY
authors.lastname: Kong
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Wang
authors.forename: Xian-Gui
authors.initials: XG
authors.lastname: Peng
authors.forename: Zhen-Zhou
authors.initials: ZZ
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D018920:Coculture Techniques; D003630:Daunorubicin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007399:Interphase / Q000187:drug effects; D019169:Jurkat Cells; D007938:Leukemia / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
chemical_list: D000903:Antibiotics, Antineoplastic; D003630:Daunorubicin