title: Shaping time: chromatin structure and the DNA replication programme.
abstract: DNA is replicated according to a precise and reproducible temporal pattern. The S-phase programme has previously been analyzed in metazoan and yeast cells using different methods: cytological chromosome banding in human cells and DNA isotopic-labeling techniques in yeast. Microarray-based approaches for the analysis of the replication programme and chromatin structure are bringing us closer to a molecular understanding of the factors that determine replication time. In this article, I assess the impact of recent investigations and compare our knowledge of DNA replication-timing controls in yeast with those of metazoans.
authors.affiliation: Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. a.d.donaldson@abdn.ac.uk
authors.forename: Anne D
authors.initials: AD
authors.lastname: Donaldson
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D016681:Genome, Fungal; D015894:Genome, Human; D006801:Humans; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D002843:Chromatin; D004271:DNA, Fungal