[Detection of EWS-WT1 fusion transcripts in paraffin-embedded tissues for desmoplastic small round cell tumor].
OBJECTIVE
To detect the EWS-WT1 chimeric mRNA in desmoplastic small round cell tumor (DSRCT) and its clinicopathological significance.


METHODS
Formalin fixed, paraffin-embedded tumor specimens from 4 examples of this entity were studied by reverse transcriptase polymerase chain reaction to detect the EWS-WT1 fusion transcripts resulting from the chromosomal translocation t(11;22)(p13;q12). The following tumor specimens were included as controls: 2 Ewing's sarcomas/primitive neuroectodermal tumors (PNET/ES), 2 alveolar rhabdomyosarcomas, and 2 lymphomas.


RESULTS
EWS-WT1 fusion transcripts were detected in 3 of 4 desmoplastic small round cell tumors but not in any other tumor types studied as controls.


CONCLUSIONS
The analysis of the EWS-WT1 fusion transcript which was performed on formalin-fixed, paraffin-embedded tissues is a sensitive and specific method in the diagnosis and differential diagnosis of DSRCT.
Yang
Yu
Y
Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
Wang
Hua
H
Wang
Yu-ping
YP
Zhang
Wei
W
Hui
Yun-zhong
YZ
Wang
Zi-hui
ZH
Zheng
Jie
J
D000008:Abdominal Neoplasms / Q000235:genetics* / Q000473:pathology; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000032:analysis*; D016612:Paraffin Embedding
D014408:Biomarkers, Tumor; C106032:EWS1-WT1 fusion protein, human; D015514:Oncogene Proteins, Fusion