Expression of inducible nitric oxide synthase mRNA and nitric oxide production during the development of liver abscess in hamster inoculated with Entamoeba histolytica.
The present study analyzed iNOS and eNOS mRNA expression and NO production during development of hepatic abscess caused by Entamoeba histolytica trophozoites. One 374-bp sequence, which displayed 88% identity to mammalian iNOS protein, was isolated from LPS-stimulated peritoneal hamster macrophages. A separate 365-bp cDNA sequence showed 99% identity with eNOS protein. iNOS mRNA was detected in hamsters during formation of amoebic liver abscesses, but not in control hamsters. eNOS mRNA expression was not modified. Serum nitrite concentration in hamsters infected with E. histolytica was 33 +/- 6 microM, in control hamsters was 20 +/- 3 microM. The study shows that iNOS mRNA expression and NO production are induced by E. histolytica trophozoites during amoebic liver abscess formation. However, in spite of iNOS mRNA expression and NO production, E. histolytica trophozoites induced liver abscess formation in hamster.
Ramírez-Emiliano
Joel
J
Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Noria Alta s/n, Col. Noria Alta, 36050, Guanajuato, Gto. México.
González-Hernández
Angélica
A
Arias-Negrete
Sergio
S
D000595:Amino Acid Sequence; D000818:Animals; D021461:Animals, Outbred Strains; D001483:Base Sequence; D002478:Cells, Cultured; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000276:immunology / Q000469:parasitology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000469:parasitology / Q000503:physiopathology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II