Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells.
BACKGROUND
Changes in the expression of galactose (Gal) alpha1-3Gal, swine lymphocyte antigen (SLA) class II and intracellular adhesion molecule (ICAM)-1 of single porcine pancreatic endocrine (PE) cells during the culture period were investigated.


METHODS
Cultured porcine PE-cells were fixed in 10% buffered formalin for histological evaluation. At 1, 3, 6, 9 and 12 weeks of culture, mRNA was obtained from porcine PE-cells so that the expression of SLA class II and ICAM-1 genes could be examined by reverse transcriptase-polymerase chain reaction.


RESULTS
The rates of Galalpha1-3Gal and SLA class II-positive cells did not decrease during the culture period, but the rates of Galalpha1-3Gal and SLA class II strongly positive cells significantly decreased. ICAM-1-positive cells were scarcely observed during the culture period. SLA class II and ICAM-1 mRNAs were detected at 1 and 3 weeks of culture, but were not detected after 6 weeks of culture.


CONCLUSIONS
These results suggest that partial reduction in the expression of these antigens could be obtained by a long-term culture.
Edamura
Kazuya
K
Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. edamura@brs.nihon-u.ac.jp
Nasu
Koko
K
Iwami
Yukiko
Y
Nishimura
Ryohei
R
Ogawa
Hiroyuki
H
Sasaki
Nobuo
N
Ohgawara
Hisako
H
D000818:Animals; D000941:Antigens / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D046508:Culture Techniques; D004703:Endocrine System / Q000166:cytology*; D005786:Gene Expression Regulation; D010179:Pancreas / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013552:Swine; D013997:Time Factors
D000941:Antigens; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger