title: [Granzymes A and B in pulmonary sarcoidosis (experimental study)].
abstract: Sarcoidosis is a systemic disease of unknown aetiology, morphologically characterized by well-formed epithelioid granulomas, which show little or no central necrosis. These may be present in any organ or tissue. The lung is the most frequently and prominently involved target. The granuloma is often very sharply demarcated from the adjacent tissue and is surrounded by a mantle of lymphocytes, which mediate lysis of target cells by various mechanisms, including exocytosis of lytic proteins, perforins and granzymes. Sarcoidosis laboratorial diagnosis is usually made by SACE and Lisozyme dosages. The granzymes A and B could be two other markers of the disease, since the sarcoidosis granuloma is rich in cytotoxic and NK cells. An ELISA Kit was used to measure Granzyme A and B in serum of a normal control group (NC) (n=30), and in two groups with lung pathology: one without sarcoidosis, disease control (DC) (n=21) and other with sarcoidosis (S) (n=11). Our results showed that SACE activity is significantly augmented in S group comparing with NC and DC, respectively: 82,6+/-32,7/31,9+/-17,8 - p=0,00017 and 82,6+/-32,7/31,9+/-17,8 - p=0,00024. Lisozyme activity is significantly augmented in S and DC groups comparing with NC. Granzyme B showed a significant decrease in DC and S groups comparing with NC. Granzyme A showed a significant decrease between S/NC groups. Our results suggest that the decrease of Granzyme A and B in sarcoidotic patients could be related to an ineffective inflammatory local response related to the formation of sarcoidosis granulomas. More studies are needed, particularly in BAL.
authors.affiliation: Fisiopatologia/Patologia Geral da Faculdade de Medicina da Universidade de Coimbra.
authors.forename: Marília
authors.initials: M
authors.lastname: Dourado
authors.forename: Joana
authors.initials: J
authors.lastname: Bento
authors.forename: Luís
authors.initials: L
authors.lastname: Mesquita
authors.forename: Alcide
authors.initials: A
authors.lastname: Marques
authors.forename: Sofia
authors.initials: S
authors.lastname: Vale-Pereira
authors.forename: Ana Bela Sarmento
authors.initials: AB
authors.lastname: Ribeiro
authors.forename: Anabela Mota
authors.initials: AM
authors.lastname: Pinto
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D053804:Granzymes; D006801:Humans; D008297:Male; D017565:Sarcoidosis, Pulmonary / Q000097:blood* / Q000201:enzymology; D012697:Serine Endopeptidases / Q000097:blood*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology
chemical_list: C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases; C509212:GZMA protein, human