Dynamics of mitochondria and mitochondrial Ca2+ near the plasma membrane of PC12 cells: a study by multimode microscopy.
The goal of this study is to examine whether there is a difference in the regulation of Ca2+ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca2+ ([Ca2+]mt) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca2+]mt near the plasma membrane increased earlier and decayed slower after high K+ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca2+]mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca2+.
Yang
De-Ming
DM
Department of Medical Research and Education, Taipei Veterans General Hospital, Republic of China.
Lin
Chung-Chih
CC
Lin
Hsia Yu
HY
Huang
Chien-Chang
CC
Tsai
Din Ping
DP
Chi
Chin-Wen
CW
Kao
Lung-Sen
LS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D048430:Cell Shape; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats
D002118:Calcium