title: Vaginal distribution of two volumes of the novel microbicide gel cellulose sulfate (2.5 and 3.5 mL).
abstract: This was a blinded crossover study to determine the in vivo distribution of 2.5 and 3.5 mL of 6% cellulose sulfate gel. This potential vaginal microbicide was mixed with dilute gadolinium chelate and inserted into the vagina. Magnetic resonance imaging was performed at baseline and repeated after 5, 20, 35 and 50 min with subjects either resting or ambulating between data acquisitions. Use of 2.5 mL with no ambulation gave low linear spread (53.1% of vaginal length) and surface contact (61.7%) at 50 min. Using 3.5 mL of gel with ambulation improved linear spread (84.5%) and surface contact (85.9%) of the gel in the same time interval without significant leakage. Most linear spreading took place in the first 5 min after insertion, although lateral spreading continued to increase with time especially when ambulation took place. Leakage and gel was not a significant problem at either volume.
authors.affiliation: Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA. kbarnhart@mail.obgyn.upenn.edu
authors.forename: Kurt T
authors.initials: KT
authors.lastname: Barnhart
authors.forename: E Scott
authors.initials: ES
authors.lastname: Pretorius
authors.forename: Alka
authors.initials: A
authors.lastname: Shaunik
authors.forename: Kelly
authors.initials: K
authors.lastname: Timbers
authors.forename: Marlina
authors.initials: M
authors.lastname: Nasution
authors.forename: Christine
authors.initials: C
authors.lastname: Mauck
mesh_terms: D000282:Administration, Intravaginal; D000293:Adolescent; D000328:Adult; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D002482:Cellulose / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D005782:Gels; D006801:Humans; D007700:Kinetics; D008279:Magnetic Resonance Imaging; D008875:Middle Aged; D014018:Tissue Distribution; D014621:Vagina / Q000737:chemistry*; D016138:Walking
chemical_list: D000890:Anti-Infective Agents; D005782:Gels; D002482:Cellulose; C020254:cellulose sulfate