Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth.
Polarized cells frequently use diffusion barriers to separate plasma membrane domains. It is unknown whether diffusion barriers also compartmentalize intracellular organelles. We used photobleaching techniques to characterize protein diffusion in the yeast endoplasmic reticulum (ER). Although a soluble protein diffused rapidly throughout the ER lumen, diffusion of ER membrane proteins was restricted at the bud neck. Ultrastructural studies and fluorescence microscopy revealed the presence of a ring of smooth ER at the bud neck. This ER domain and the restriction of diffusion for ER membrane proteins through the bud neck depended on septin function. The membrane-associated protein Bud6 localized to the bud neck in a septin-dependent manner and was required to restrict the diffusion of ER membrane proteins. Our results indicate that Bud6 acts downstream of septins to assemble a fence in the ER membrane at the bud neck. Thus, in polarized yeast cells, diffusion barriers compartmentalize the ER and the plasma membrane along parallel lines.
Luedeke
Cosima
C
Biology Department, Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), ETH-Hönggerberg, 8093 Zürich, Switzerland.
Frei
Stéphanie Buvelot
SB
Sbalzarini
Ivo
I
Schwarz
Heinz
H
Spang
Anne
A
Barral
Yves
Y
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D002451:Cell Compartmentation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049108:Cell Enlargement; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D016764:Cell Polarity / Q000502:physiology*; D004058:Diffusion; D018871:Endoplasmic Reticulum, Smooth / Q000378:metabolism* / Q000648:ultrastructure; D036681:Fluorescence Recovery After Photobleaching; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008840:Microfilament Proteins / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D021381:Protein Transport / Q000502:physiology; D000069816:SEC Translocation Channels; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
C107727:BUD6 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008840:Microfilament Proteins; D000069816:SEC Translocation Channels; C499383:SEC61 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins