Sp1 elements in SULT2B1b promoter and 5'-untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition.
The steroid/sterol sulfotransferase gene (SULT2B1) encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human SULT2B1 gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5'-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibition.
Lee
Young C
YC
Section on Steroid Regulation, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892-4510, USA.
Higashi
Yuko
Y
Luu
Chu
C
Shimizu
Chikara
C
Strott
Charles A
CA
D020121:5' Untranslated Regions / Q000235:genetics*; D000595:Amino Acid Sequence; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D015603:Keratinocytes / Q000201:enzymology; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D020218:Response Elements; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D051704:Sp2 Transcription Factor; D015238:Sulfotransferases / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D024363:Transcription Initiation Site; D015533:Transcriptional Activation
D020121:5' Untranslated Regions; D004268:DNA-Binding Proteins; D056572:Histone Deacetylase Inhibitors; D007527:Isoenzymes; D012333:RNA, Messenger; C497119:SP2 protein, human; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D051704:Sp2 Transcription Factor; D008156:Luciferases; D015238:Sulfotransferases; C433248:SULT2B1 protein, human