title: Exploring global motions and correlations in the ribosome.
abstract: We studied slower global coupled motions of the ribosome with half a microsecond of coarse-grained molecular dynamics. A low-resolution anharmonic network model that allows for the evolution of tertiary structure and long-scale sampling was developed and parameterized. Most importantly, we find that functionally important movements of L7/L12 and L1 lateral stalks are anticorrelated. Other principal directions of motions include widening of the tRNA cleft and the rotation of the small subunit which occurs as one block and is in phase with the movement of L1 stalk. The effect of the dynamical correlation pattern on the elongation process is discussed. Small fluctuations of the 3' tRNA termini and anticodon nucleotides show tight alignment of substrates for the reaction. Our model provides an efficient and reliable way to study the dynamics of large biomolecular systems composed of both proteins and nucleic acids.
authors.affiliation: Department of Chemistry and Biochemistry and Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, California, USA. joanna@icm.edu.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Trylska
authors.forename: Valentina
authors.initials: V
authors.lastname: Tozzini
authors.forename: J Andrew
authors.initials: JA
authors.lastname: McCammon
mesh_terms: D000926:Anticodon / Q000737:chemistry; D001703:Biophysics / Q000379:methods*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D009711:Nucleotides / Q000737:chemistry; D010444:Peptide Elongation Factor Tu / Q000737:chemistry; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry*; D012984:Software; D013997:Time Factors
chemical_list: D000926:Anticodon; D009711:Nucleotides; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D012343:RNA, Transfer; D010444:Peptide Elongation Factor Tu