Evaluation of a new molecular system for simultaneous identification of four Enterococcus species and their glycopeptide resistance genotypes.
We compared the performances of a new DNA-based strip assay and the VITEK 2 system for identification of 105 enterococcal strains and differentiation of their van gene-associated resistance levels. Both methods provided excellent results. The molecular assay showed advantages in time to result for identification of van-associated genes.
Eigner
U
U
Department of Microbiology and Hygiene, Laboratory Group Limbach, Heidelberg, Germany.
Fahr
A
A
Weizenegger
M
M
Witte
W
W
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D015342:DNA Probes; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D011934:Reagent Strips; D013045:Species Specificity; D017334:Teicoplanin / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D011934:Reagent Strips; D017334:Teicoplanin; D014640:Vancomycin