[Detection of gene expression alteration of myeloma cells treated with arsenic trioxide].
OBJECTIVE
To investigate the effect of arsenic trioxide on multiple myeloma (MM) cell gene expression and explore the molecular mechanism of arsenic trioxide therapy for MM.


METHODS
U266 cells were divided into two groups, group A as control group and group B as test group. Cells were cultured for 48 hours, and total RNA and mRNA were extracted. Suppression subtractive hybridization (SSHs) was performed to distinguish the differentially expressed genes. The products were cloned into pGEM-T Easy Vector, and transfected into the competent host JM109 to construct two subtractive libraries. Positive colonies were selected by blue-white screening, and the plasmids were extracted. Homologous comparison was conducted in GenBank.


RESULTS
Five downregulated clones were isolated in the first SSH: (1) Aminopeptidase N, (2) Homosapiens tumor translationally-controlled protein 1, (3) Human ATP synthetase A chain, (4) Signal recognition particle A10, (5) Mitochondrial ATP synthetase/ATPase subunit 6. Four upregulated clones were isolated in the second SSH: (1) Calcium-binding protein A10, (2) Keratin 6A, (3) 45 kD MIP repetitive element containing splicing factor and (4) poly(A)-binding protein.


CONCLUSIONS
Arsenic trioxide exerts proliferation inhibition and apoptosis induction on MM cells by regulating genes expression.
Li
Cui-Lian
CL
Department of Hematology, Chao Yang Hospital, Capital University of Medical Science, Beijing 100020, China.
Chen
Shi-Lun
SL
Chen
Wen-Ming
WM
Liu
Jing-Zhong
JZ
Xiao
Bai
B
Zhang
Hai-Bo
HB
D000970:Antineoplastic Agents / Q000494:pharmacology; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015723:Gene Library; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology; D010087:Oxides / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide