Selective cytolysis of tumor cells by mumps virus S79.
We studied the sensitivity of several human cancer cell strains (HeLa, HT1080, SPC-A1, and ACHN) and normal cell strains (MRC-5 and Wish) to mumps virus (MuV) S79, a live attenuated vaccine strain. These cells exhibited a differential sensitivity to infection with MuV, and the susceptible sequences were ACHN > HeLa > HT1080 > SPC-A1 > Wish > MRC-5. In experiments in vivo, nude mice with HT1080 fibrosarcoma xenografts were randomly divided into three groups for intratumoral treatment with MuVS79, UV-inactivated MuVS79, and PBS. At 10(7) PFU, live MuVS79 injection caused in 7 of 9 mice complete regression by day 15 while rapid tumor growth occurred in all 9 mice treated with PBS. Rapid tumor progression also occurred in all 8 mice treated with UV-inactivated virus; however, tumor growth was delayed in the logarithmic phase relative to the PBS-treated tumors.
Yan
Yin-Fang
YF
Institute of Medical Virology, Wuhan University, Wuhan, People's Republic of China. yanyinfang11@yahoo.com.ch
Chen
Xiao
X
Zhu
Ying
Y
Wu
Jian-Guo
JG
Dong
Chang-Yuan
CY
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D005354:Fibrosarcoma / Q000628:therapy* / Q000821:virology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009109:Mumps virus / Q000502:physiology*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology / Q000628:therapy* / Q000821:virology*; D014779:Virus Replication