Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction.
Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The I-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate.
Takeda
Seiji
S
Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8 Kita-ku, Sapporo 060-8628, Japan. takeda@nano.eng.hokudai.ac.jp
Sbagyo
Agus
A
Sakoda
Yoshihiro
Y
Ishii
Atsushi
A
Sawamura
Makoto
M
Sueoka
Kazuhisa
K
Kida
Hiroshi
H
Mukasa
Koichi
K
Matsumoto
Kazuhiko
K
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics; D008839:Microelectrodes; D037742:Nanotubes, Carbon; D009532:Nickel / Q000378:metabolism
D000914:Antibodies, Viral; D006388:Hemagglutinins; D037742:Nanotubes, Carbon; D009532:Nickel