title: Nicotine upregulates its own receptors through enhanced intracellular maturation.
abstract: Chronic exposure to nicotine elicits upregulation of high-affinity nicotinic receptors in the smoker's brain. To address the molecular mechanism of upregulation, we transfected HEK293 cells with human alpha4beta2 receptors and traced the subunits throughout their intracellular biosynthesis, using metabolic labeling and immunoprecipitation techniques. We show that high-mannose glycosylated subunits mature and assemble into pentamers in the endoplasmic reticulum and that only pentameric receptors reach the cell surface following carbohydrate processing. Nicotine is shown to act inside the cell and to increase the amount of beta subunits immunoprecipitated by the conformation-dependent mAb290, indicating that nicotine enhances a critical step in the intracellular maturation of these receptors. This effect, which also takes place at concentrations of nicotine found in the blood of smokers upon expression of alpha4beta2 in SH-SY5Y neuroblastoma cells, may play a crucial role in nicotine addiction and possibly implement a model of neural plasticity.
authors.affiliation: CNRS URA D2182 Récepteurs et Cognition, Institut Pasteur, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Sallette
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Pons
authors.forename: Anne
authors.initials: A
authors.lastname: Devillers-Thiery
authors.forename: Martine
authors.initials: M
authors.lastname: Soudant
authors.forename: Lia
authors.initials: L
authors.lastname: Prado de Carvalho
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Changeux
authors.forename: Pierre Jean
authors.initials: PJ
authors.lastname: Corringer
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001345:Autoradiography / Q000379:methods; D001667:Binding, Competitive / Q000187:drug effects; D015153:Blotting, Western / Q000379:methods; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D004079:Dihydro-beta-Erythroidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005455:Fluorescent Antibody Technique / Q000379:methods; D006031:Glycosylation; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D042541:Intracellular Space / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D008954:Models, Biological; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D018408:Patch-Clamp Techniques / Q000379:methods; D011485:Protein Binding / Q000187:drug effects; D021122:Protein Subunits / Q000276:immunology; D011725:Pyridines / Q000493:pharmacokinetics; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods; D014316:Tritium / Q000493:pharmacokinetics; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000906:Antibodies; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018722:Nicotinic Agonists; D018733:Nicotinic Antagonists; D021122:Protein Subunits; D011725:Pyridines; D011978:Receptors, Nicotinic; C403531:nicotinic receptor alpha4beta2; D014316:Tritium; D004079:Dihydro-beta-Erythroidine; D009538:Nicotine; D002217:Carbachol; C082748:epibatidine