Direct MS/MS analysis of proteins blotted on membranes by a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer.
We constructed a system for the microscale identification of membrane-blotted proteins by proteolytic digestion using an instrument developed with piezoelectric chemical inkjet technology and MS/MS analyses of the resulting peptides with a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer (MALDI-QIT-TOF MS). Using this system, bovine serum albumin was clearly identified at levels less than 100 fmol, and proteins from an Escherichia coli extract were also identified by an MS/MS ion search.
Nakanishi
Tsuyoshi
T
Life Science Laboratory, Analytical & Measuring Instruments Division, Shimadzu Corporation,1, Nishinokyo-Kuwabaracho, Nakagyo-ku Kyoto 604-8511, Japan.
Ohtsu
Iwao
I
Furuta
Masaru
M
Ando
Eiji
E
Nishimura
Osamu
O
D015153:Blotting, Western; D029968:Escherichia coli Proteins / Q000032:analysis; D008832:Microchemistry; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D011506:Proteins; D012710:Serum Albumin, Bovine