title: [Effects of alcohol on the expression of heat shock protein 70 and super microstructure of astrocytes in rat embryonic brain].
abstract: OBJECTIVE
  To explore effects of alcohol on the expression of heat shock protein 70 (HSP70) and super microstructure of astrocytes.
  
  
  METHODS
  Different dosage of alcohol (1, 5, 25, 50mmol/L) was administered to astrocytes of rat embryos. Effects of alcohol on expression of HSP70 were studied by immunocytochemistry, and changes of super microstructure of astrocytes were observed by electron microscope.
  
  
  RESULTS
  The results showed that with increase of alcohol dosage, the expression of HSP70 increased, hepatin granule and survival astrocytes in 25mmol/L and 50mmol/L dosage group decreased, cell membrane and mitochondrion were damaged, and even apoptosis and necrosis occurred.
  
  
  CONCLUSIONS
  It suggested that alcohol could increase expression of HSP of astrocytes, which could depress the expression of normal function protein, and mainly impaired cell membrane and mitochondrion, which could lead to apoptosis and necrosis.
authors.affiliation: School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Qu
authors.forename: Seng
authors.initials: S
authors.lastname: Ya
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: Tianbao
authors.initials: T
authors.lastname: Zhang
authors.forename: Songhui
authors.initials: S
authors.lastname: Jiang
authors.forename: Huigang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018840:HSP70 Heat-Shock Proteins; D000431:Ethanol