Heat shock protein 27 expression in patients with chronic liver damage.
The aim of this study was to evaluate a possible relationship between lymphomonocyte expression of heat shock proteins (HSP) 60/27 and plasma levels of pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6) and markers of antioxidant/oxidative status [glutathione (GSH), alpha glutathione-S-transferase activity (alpha GST), malonyldialdeyde (MDA), 4-hydroxinonenal (4-HNE), and S-nitrosothiols (S-NO)] in patients with chronic liver diseases. Entered into the study were 47 subjects: 10 healthy controls, 16 patients with HCV-related chronic hepatitis (CH), and 16 patients with HCV-related and 5 with alcohol-related liver cirrhosis (10 Child A and 11 Child B+C). HSP60 was clearly expressed only in 5% of patients and lowly in the control group. HSP27 was clearly expressed in 46.7% of CH and 71.4% of cirrhotic patients but was lowly present in healthy subjects. A significant difference was found between patients with a low expression of HSP27 (negative patients) and those with a high HSP27 expression (positive patients) of plasma levels both of antioxidants (GSH, p < 0.05), and of markers of enhanced production of free radicals and cytokines (alpha GST, TNF-alpha and IL-6, p < 0.05; MDA, 4-HNE and S-NO, p < 0.01) as well as for alcohol use and degree of liver impairment. The present data are the first showing that, particularly in conditions of enhanced oxidative stress, lymphomonocytes from liver disease patients present an increased expression of HSP27.
Federico
Alessandro
A
Centro Interuniversitario per Ricerche su Alimenti, Nutrizione e Apparato Digerente (CIRANAD), Cattedra di Gastroenterologia, II Università di Napoli, via Pansini 5, Napoli, Italy. alessandro.federico@unina2.it
Tuccillo
Concetta
C
Terracciano
Fulvia
F
D'Alessio
Carmela
C
Galdiero
Marilena
M
Finamore
Emiliana
E
D'Isanto
Marina
M
Peluso
Lucia
L
Del Vecchio Blanco
Camillo
C
Loguercio
Carmela
C
D000328:Adult; D000368:Aged; D000428:Alcohol Drinking; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002908:Chronic Disease; D005260:Female; D005786:Gene Expression Regulation; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction
D000975:Antioxidants; D015415:Biomarkers; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins