Somatic hypermutation and mismatch repair in non-B cells.
Mismatch repair contributes to hypermutation in B lymphocytes, both by increasing the frequency of mutations and by changing the mutational patterns. In this paper, we investigated whether or not mismatch repair influences activation-induced cytidine deaminase (AID)-mediated hypermutation in a non-B lymphocyte line. We did so by regulating expression of MutL homologue MLH 1, which is essential in mismatch repair, in a kidney cell line that had been transduced by an AID-containing vector. Whether or not MLH1 was expressed, we found no difference in the mutation rates of an indicator gene. We conclude that in order to contribute to hypermutation, mismatch repair needs additional factors that are present in activated B lymphocytes, but absent in the cell line investigated.
Klasen
Maik
M
Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0670, USA.
Spillmann
Freia J X
FJ
Marra
Giancarlo
G
Cejka
Petr
P
Wabl
Matthias
M
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D020137:Base Pair Mismatch / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins; D043525:Cytosine Deaminase / Q000096:biosynthesis / Q000235:genetics; D004260:DNA Repair / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D051379:Mice; D000070957:MutL Protein Homolog 1; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D027041:Somatic Hypermutation, Immunoglobulin / Q000276:immunology / Q000502:physiology*; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C501486:MLH1 protein, human; C501485:Mlh1 protein, mouse; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D043525:Cytosine Deaminase; D000070957:MutL Protein Homolog 1