title: Pseudomonas aeruginosa infection in the hypoventilated middle ear: an experimental model.
abstract: CONCLUSIONS
  This is a suitable model for studying different aspects of the pathophysiology of chronic suppurative otitis media.
  
  
  OBJECTIVE
  To analyze the methodological features of an animal model of chronic suppurative otitis media induced by intratympanic inoculation of Pseudomonas aeruginosa.
  
  
  METHODS
  Otitis media was induced by inoculation of P. aeruginosa through the inferior aspect of the rat bulla and cauterization of the Eustachian tube via a transpalatal approach. Inspection of the tympanic membrane, culturing of middle ear effusion and processing of the temporal bones for light microscopy were performed.
  
  
  RESULTS
  Abnormal otomicroscopic findings and persistence of infection were correlated with the histopathological changes found in middle ear tissues.
authors.affiliation: Department of Otorhinolaryngology, Hospital Puerta de Hierro, Madrid, Spain. atrinid@yahoo.es
authors.forename: Almudena
authors.initials: A
authors.lastname: Trinidad
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez-Camacho
authors.forename: Jose Ramón
authors.initials: JR
authors.lastname: García-Berrocal
authors.forename: José María
authors.initials: JM
authors.lastname: Verdaguer
authors.forename: Javier
authors.initials: J
authors.lastname: Vicente
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Pinilla
mesh_terms: D000818:Animals; D001768:Blister / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000382:microbiology / Q000473:pathology; D008297:Male; D008853:Microscopy; D010034:Otitis Media with Effusion / Q000382:microbiology* / Q000473:pathology*; D038781:Otoscopy; D011552:Pseudomonas Infections / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar; D013701:Temporal Bone / Q000382:microbiology / Q000473:pathology; D014432:Tympanic Membrane / Q000382:microbiology / Q000473:pathology