title: Pancreatic cancer-associated diabetes mellitus: an open field for proteomic applications.
abstract: BACKGROUND
  Diabetes mellitus is associated with pancreatic cancer in more than 80% of the cases. Clinical, epidemiological, and experimental data indicate that pancreatic cancer causes diabetes mellitus by releasing soluble mediators which interfere with both beta-cell function and liver and muscle glucose metabolism.
  
  
  METHODS
  We analysed, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), a series of pancreatic cancer cell lines conditioned media, pancreatic cancer patients' peripheral and portal sera, comparing them with controls and chronic pancreatitis patients' sera.
  
  
  RESULTS
  MALDI-TOF analysis of pancreatic cancer cells conditioned media and patients' sera indicated a low molecular weight peptide to be the putative pancreatic cancer-associated diabetogenic factor. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of tumor samples from diabetic and non-diabetic patients revealed the presence of a 1500 Da peptide only in diabetic patients. The amino acid sequence of this peptide corresponded to the N-terminal of an S-100 calcium binding protein, which was therefore suggested to be the pancreatic cancer-associated diabetogenic factor.
  
  
  CONCLUSIONS
  We identified a tumor-derived peptide of 14 amino acids sharing a 100% homology with an S-100 calcium binding protein, which is probably the pancreatic cancer-associated diabetogenic factor.
authors.affiliation: Department of Laboratory Medicine, University of Padova, Via Giustiniani 2, Padova 35128, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Basso
authors.forename: Eliana
authors.initials: E
authors.lastname: Greco
authors.forename: Paola
authors.initials: P
authors.lastname: Fogar
authors.forename: Piero
authors.initials: P
authors.lastname: Pucci
authors.forename: Angela
authors.initials: A
authors.lastname: Flagiello
authors.forename: Goretta
authors.initials: G
authors.lastname: Baldo
authors.forename: Silvia
authors.initials: S
authors.lastname: Giunco
authors.forename: Anna
authors.initials: A
authors.lastname: Valerio
authors.forename: Filippo
authors.initials: F
authors.lastname: Navaglia
authors.forename: Carlo-Federico
authors.initials: CF
authors.lastname: Zambon
authors.forename: Sergio
authors.initials: S
authors.lastname: Pedrazzoli
authors.forename: Mario
authors.initials: M
authors.lastname: Plebani
mesh_terms: D000818:Animals; D048909:Diabetes Complications / Q000175:diagnosis / Q000209:etiology* / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D010190:Pancreatic Neoplasms / Q000150:complications* / Q000175:diagnosis / Q000378:metabolism*; D040901:Proteomics
chemical_list: D005947:Glucose