[Microbiological external quality assurance program (MEQAP)--evaluation of results obtained in sanitary epidemiological laboratories in 2002].
The aim of this study was to evaluate reliability of identification and determination of sensitivity to antibiotics and chemotherapeutics of some Gram positive cocci strains in 34 sanitary-epidemiological stations. All laboratories engaged in this study received 3 strains: S. aureus (S. aureus SC+ CF+, resistant to methicillin., or S. aureus SC - CF+, sensitive to methicillin), coagulase-negative staphylococci (S. epidermidis or S. haemolyticus or S. saprophyticus) and Enterococcus sp. (E. faecalis HLAR-positive or E. faecalis HLAR-negative or E. faecium or E. gallinarum). All these strains previously were identified in Department of Bacteriology of National Institute of Hygiene. Of the 68 staphylococci strains tested, 66 isolates were correctly identified. Among the 34 enterococcal strains studied the greatest difficulty in identification was caused by E. gallinarum strain--(8 out of 13 strains were incorrectly recognised). The determination of the sensitivity of the control strains to antibiotics and chemotherapeutic agents, generally was performed correctly in accordance with to the NCCLS and national recommendations. Some incorrect results of the antibiograms were caused by an erroneous interpretation of the zones of inhibition.
Kochman
Maria
M
Zakład Bakteriologii PZH w Warszawie.
Piekarska
Katarzyna
K
Lawrynowicz-Paciorek
Maja
M
D016983:Enterococcus / Q000302:isolation & purification; D006095:Gram-Positive Cocci / Q000302:isolation & purification*; D006801:Humans; D007755:Laboratories, Hospital / Q000592:standards*; D008826:Microbial Sensitivity Tests; D011044:Poland; D015397:Program Evaluation; D011785:Quality Assurance, Health Care / Q000592:standards*; D015203:Reproducibility of Results; D013045:Species Specificity; D013210:Staphylococcus / Q000302:isolation & purification