title: Density functional theory calculations on the dielectric constant dependence of the oxidation potential of chlorophyll: implication for the high potential of P680 in photosystem II.
abstract: The primary donor chlorophyll (Chl) of photosystem II (PSII), P680, has an extremely high oxidation redox potential (E(ox)) of approximately 1.2 V, which is essential for photosynthetic water oxidation. The mechanism for achieving a high potential such as that of P680 has been one of the central questions in photosynthesis research. Here, we have examined the dielectric constant (epsilon) dependence of the E(ox) of monomer Chl using density functional theory calculations with the polarizable continuum model. The calculated E(ox) of a model Chl compound exhibited a sharp increase with a decrease in epsilon in the relatively low epsilon region (epsilon < 5). In contrast, in the higher-epsilon region, E(ox) was rather insensitive to epsilon and converged to a constant value at very high epsilon values. This tendency in the high-epsilon region explains the experimental E(ox) values of isolated Chl a that have been observed in a relatively narrow range of 0.74-0.93 V. The E(ox) of Chl in an ideal hydrophobic protein was estimated to be approximately 1.4 V at an epsilon value of 2. This value indicates that Chl in a hydrophobic environment originally has a high E(ox) that is sufficient for oxidizing water (E(ox) = 0.88 V at pH 6). On the basis of the reported X-ray crystallographic structures, the protein environment of P680 in PSII was estimated to be more hydrophobic than that of the primary donors in bacterial reaction centers. It is therefore suggested that the low-dielectric environment around P680 is one of the major factors in its very high E(ox), and thus, introducing nonpolar amino acids into the binding pocket of P680 was an important process in the evolution of PSII.
authors.affiliation: Laboratory for Photo-Biology (I), RIKEN Photodynamics Research Center, Aoba, Sendai, Miyagi 980-0845, Japan. kojihase@ postman.riken.go.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Takumi
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D008958:Models, Molecular; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism; D011199:Potentiometry
chemical_list: D045332:Photosystem II Protein Complex; D002734:Chlorophyll; C025167:P-680