Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis.
Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF.
Lacroix
Marlene
M
Laboratoire des IMRCP, UMR 5623, ERT 1046, Université Paul Sabatier, Toulouse, France.
Poinsot
Véréna
V
Fournier
Clara
C
Couderc
François
F
D000230:Adenocarcinoma / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D003110:Colonic Neoplasms / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry; D006801:Humans; D007834:Lasers; D009281:Naphthalenes / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013373:Substance P / Q000032:analysis
C413243:5-furoylquinoline-3-carboxaldehyde; D001565:Benzoates; D005456:Fluorescent Dyes; D005663:Furans; D009281:Naphthalenes; D010455:Peptides; D011506:Proteins; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde; D013373:Substance P; C027717:2,3-naphthalenedicarboxaldehyde; D016650:Fluorescein-5-isothiocyanate