title: Investigation of Epstein-Barr virus DNA in formalin-fixed and paraffin- embedded breast cancer tissues.
abstract: OBJECTIVE
  To investigate etiological role of Epstein-Barr virus (EBV) DNA in breast cancer.
  
  
  METHODS
  The presence of EBV DNA in 57 breast cancer tissues was investigated with a sensitive PCR assay. The breast cancer tissues were from invasive ductular (n=28), lobular (n=20) and other miscellaneous carcinomas (n=9). Tissues from normal breasts and patients with various benign breast diseases (n=55): fibrocystic disease (n=34), fibroadenoma (n=16), hyperplasia, and granulomatous mastitis (n=5), were used as control samples.
  
  
  RESULTS
  EBV DNA was detected in 13 (23%) cancerous tissues (7 ductular, 4 lobular, 2 other carcinoma) and 19 (35%) in the control tissues. The difference between EBV presence in malignant and benign tissues was not statistically significant (p>0.05).
  
  
  CONCLUSIONS
  The presence of EBV DNA was detected almost equally in both breast cancer and normal tissues, which indicates no etiological role for EBV in breast cancer. We suggest further etiological studies.
authors.affiliation: Department of Infectious Diseases, Firat University Medical School, Elazig, Turkey. akalkan61@hotmail.com
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kalkan
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozdarendeli
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Bulut
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Yekeler
authors.forename: Bengu
authors.initials: B
authors.lastname: Cobanoglu
authors.forename: Mehmet Z
authors.initials: MZ
authors.lastname: Doymaz
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D001943:Breast Neoplasms / Q000209:etiology / Q000821:virology*; D018275:Carcinoma, Lobular / Q000209:etiology / Q000821:virology*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005557:Formaldehyde; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008875:Middle Aged; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014421:Turkey
chemical_list: D004279:DNA, Viral; D005557:Formaldehyde