Oxidative damage in mitochondrial DNA is not extensive.
Since 1988 several research groups have reported greater levels of oxidative damage in mitochondrial DNA than in nuclear DNA, while others have suggested that the greater damage in mtDNA might be due to artifactual oxidation. The popular theory that mtDNA is more heavily damaged in vivo than nDNA does not stand on firm ground. Using an improved GC-MS method and pure mtDNA, our analyses revealed that the damage level in mtDNA is not higher, and may be somewhat lower, than that in nDNA.
Lim
Kok Seong
KS
Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117597.
Jeyaseelan
Kandiah
K
Whiteman
Matthew
M
Jenner
Andrew
A
Halliwell
Barry
B
D000818:Animals; D004249:DNA Damage / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D019800:Phenol; D051381:Rats; D017207:Rats, Sprague-Dawley
D004272:DNA, Mitochondrial; D019800:Phenol