title: STRUBBELIG defines a receptor kinase-mediated signaling pathway regulating organ development in Arabidopsis.
abstract: An open question remains as to what coordinates cell behavior during organogenesis, permitting organs to reach their appropriate size and shape. The Arabidopsis gene STRUBBELIG (SUB) defines a receptor-mediated signaling pathway in plants. SUB encodes a putative leucine-rich repeat transmembrane receptor-like kinase. The mutant sub phenotype suggests that SUB affects the formation and shape of several organs by influencing cell morphogenesis, the orientation of the division plane, and cell proliferation. Mutational analysis suggests that the kinase domain is important for SUB function. Biochemical assays using bacterially expressed fusion proteins indicate that the SUB kinase domain lacks enzymatic phosphotransfer activity. Furthermore, transgenes encoding WT and different mutant variants of SUB were tested for their ability to rescue the mutant sub phenotype. These genetic data also indicate that SUB carries a catalytically inactive kinase domain. The SUB receptor-like kinase may therefore signal in an atypical fashion.
authors.affiliation: Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8008 Zurich, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Chevalier
authors.forename: Martine
authors.initials: M
authors.lastname: Batoux
authors.forename: Lynette
authors.initials: L
authors.lastname: Fulton
authors.forename: Karen
authors.initials: K
authors.lastname: Pfister
authors.forename: Ram Kishor
authors.initials: RK
authors.lastname: Yadav
authors.forename: Maja
authors.initials: M
authors.lastname: Schellenberg
authors.forename: Kay
authors.initials: K
authors.lastname: Schneitz
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D017403:In Situ Hybridization; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D011494:Protein Kinases; D020794:Receptor Protein-Tyrosine Kinases; C503317:SUB protein, Arabidopsis