title: Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene.
abstract: In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
authors.affiliation: Department of Microbiology, Konkuk University, Chungju, Chungchongbuk-Do, Korea.
authors.forename: Hyo-Soon
authors.initials: HS
authors.lastname: Park
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Hong
authors.forename: Tae-Kyu
authors.initials: TK
authors.lastname: Park
authors.forename: Tae-Yung
authors.initials: TY
authors.lastname: Kim
authors.forename: Won-Jong
authors.initials: WJ
authors.lastname: Jang
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Park
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000453:epidemiology* / Q000276:immunology; D017422:Sequence Analysis, DNA; D014437:Typhus, Endemic Flea-Borne / Q000453:epidemiology* / Q000276:immunology
chemical_list: D018834:Chaperonin 60; D004275:DNA, Ribosomal