TLRs and NODs mRNA expression pattern in healthy mouse eye.
OBJECTIVE
To look for TLR and NOD mRNA expression in the healthy eye and in other immune privileged and non-immune privileged mouse organs.


METHODS
Semiquantitative RT-PCR was performed to look for TLR1-9 and NOD1 and NOD2 mRNA expressions in the whole eye, in the anterior (AP) and posterior (PP) portions of the eye, in corneal fibroblasts (CF) and in ovary, brain, testis, heart, lung, and spleen.


RESULTS
All the TLR mRNAs were expressed in the whole eye of Balb/c mice. NIH and C57BL/6 did not express TLR9 and TLR8, respectively. NIH expressed higher levels of TLR1, 2, 3, and 6 than the other strains. C57BL/6 expressed the lowest levels of all TLRs. TLR9, 5, and 4 were the less expressed in all strains. All TLRs were expressed in Balb/c PP and TLR1 was not expressed in AP. In NIH and Balb/c CF the majority of TLRs were overexpressed with LPS. In testis, expression of most TLRs was absent. Non-immune privileged organs expressed most of the TLRs. All the organs expressed NOD1 and NOD2. In PP NOD2 was not expressed.


CONCLUSIONS
TLRs and NODs are expressed in the eye, and could have an important role in the innate immunity.
Rodríguez-Martínez
S
S
Laboratorio de Microbiología General, Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas del Instituto Politecnico Nacional, Carpio y Plan de Ayala, México, México.
Cancino-Díaz
M E
ME
Jiménez-Zamudio
L
L
García-Latorre
E
E
Cancino-Díaz
J C
JC
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005123:Eye / Q000737:chemistry / Q000276:immunology; D005136:Eye Proteins / Q000032:analysis* / Q000276:immunology; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000276:immunology; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D053474:Nod1 Signaling Adaptor Protein; D053473:Nod2 Signaling Adaptor Protein; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D051194:Toll-Like Receptor 1; D051200:Toll-Like Receptor 8; D051193:Toll-Like Receptors
D048868:Adaptor Proteins, Signal Transducing; D005136:Eye Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein; D053473:Nod2 Signaling Adaptor Protein; C495945:Nod2 protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051194:Toll-Like Receptor 1; D051200:Toll-Like Receptor 8; D051193:Toll-Like Receptors