Interleukin-1beta (IL-1beta) induces a crosstalk between cAMP and ceramide signaling pathways in thyroid epithelial cells.
Interleukin-1 beta (IL-1beta) is an important regulator of the thyroid cell function. This cytokine has been largely described to trigger an important biological signaling cascade: the sphingomyelin/ceramide pathway. In this report, we show that IL-1beta induces the transient activation of a neutral sphingomyelinase in porcine thyroid cells. Moreover, IL-1beta and ceramides are demonstrated to inhibit the TSH-induced cAMP production via the implication of alphaGi subunit of the adenylyl cyclase system. This crosstalk between cAMP and ceramide pathways constitutes a preponderant process in the TSH-controlled differentiation state of thyrocytes. All these results argue for the involvement of ceramides and IL-1beta in the thyroid function regulation, leading to a cell dedifferentiated state.
Schneider
C
C
Laboratoire de Biochimie, Université de Reims Champagne-Ardenne, IFR 53 Biomolécules, UMR CNRS 6198 Matrice extracellulaire et régulations cellulaires, UFR Sciences Exactes et Naturelles, Moulin de la Housse, BP 1039, 51687 Reims cedex 2, France.
Rath
G M
GM
Delorme
N
N
El Btaouri
H
H
Hornebeck
W
W
Martiny
L
L
D000818:Animals; D002518:Ceramides / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D037342:Pertussis Toxin / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
D002518:Ceramides; D007375:Interleukin-1; D000242:Cyclic AMP; D037342:Pertussis Toxin; D013108:Sphingomyelin Phosphodiesterase