title: DREF is required for efficient growth and cell cycle progression in Drosophila imaginal discs.
abstract: Based on overexpression studies and target gene analyses, the transcription factor DNA replication-related element factor (DREF) has been proposed to regulate growth and replication in Drosophila melanogaster. Here we present loss-of-function experiments to analyze the contribution of DREF to these processes. RNA interference-mediated extinction of DREF function in vivo demonstrates a requirement for the protein for normal progression through the cell cycle and consequently for growth of imaginal discs and the derived adult organs. We show that DREF regulates the expression of genes that are required for the transition of imaginal disc cells through S phase. In conditions of suppressed apoptosis, DREF activation can cause overgrowth of developing organs. These data establish DREF as a global regulator of transcriptional programs that mediate cell proliferation and organ growth during animal development.
authors.affiliation: Department of Biomedical Genetics, The Aab Institute of Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 633, Rochester, NY 14642, USA.
authors.forename: Joogyung
authors.initials: J
authors.lastname: Hyun
authors.forename: Heinrich
authors.initials: H
authors.lastname: Jasper
authors.forename: Dirk
authors.initials: D
authors.lastname: Bohmann
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology*; D049109:Cell Proliferation; D004330:Drosophila / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000502:physiology*; D009154:Mutation; D009929:Organ Size / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D016196:S Phase; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D018797:Cell Cycle Proteins; C099494:Dref protein, Drosophila; D029721:Drosophila Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D014157:Transcription Factors