Nociceptin system does not affect MAC of volatile anaesthetics.
BACKGROUND
Nociceptin is the endogenous agonist of the opioid receptor-like (ORL) 1 receptor (NOP), and both nociceptin and NOP are widely expressed in the brain and spinal cord, which are target organs of general anaesthetics. As nociceptin has been reported to be involved in modulating pain mechanisms and stress responses, it is possible that the activity of the nociceptin system affects the anaesthetic potency of general anaesthetics. To address this possibility, we investigated the minimum alveolar concentrations (MACs) of various volatile anaesthetics in nociceptin receptor knockout mice (NOP-/-) and wild-type mice (NOP+/+).


METHODS
We used male NOP-/- mice and NOP+/+ mice. MACs for halothane, isoflurane and sevoflurane were determined by the tail-clamp method.


RESULTS
MACs for halothane, isoflurane and sevoflurane in NOP-/- mice were 1.60 (SD 0.06), 1.68 (0.08) and 3.36 (0.07)%, respectively. In NOP+/+ mice, MACs for halothane, isoflurane and sevoflurane were 1.59 (SD 0.07), 1.72 (0.07) and 3.38 (0.09)%, respectively.


CONCLUSIONS
MACs in NOP-/- mice did not significantly differ from those in NOP+/+ mice for halothane, isoflurane and sevoflurane. This result suggests that the nociceptin system does not affect the anaesthetic potency of volatile anaesthetics.
Himukashi
S
S
Department of Anaesthesia, Kyoto University Hospital, Kyoto, Japan. himukash@kuhp.kyoto-u.ac.jp
Miyazaki
Y
Y
Takeshima
H
H
Koyanagi
S
S
Mukaida
K
K
Shichino
T
T
Uga
H
H
Fukuda
K
K
D018685:Anesthetics, Inhalation / Q000493:pharmacokinetics* / Q000494:pharmacology*; D000818:Animals; D006221:Halothane / Q000494:pharmacology; D007530:Isoflurane / Q000494:pharmacology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018847:Opioid Peptides / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011957:Receptors, Opioid / Q000235:genetics / Q000502:physiology*; D000077149:Sevoflurane; D000094942:Nociceptin Receptor; D000097629:Nociceptin
D018685:Anesthetics, Inhalation; D006221:Halothane; D007530:Isoflurane; D008738:Methyl Ethers; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000077149:Sevoflurane; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722287:Oprl1 protein, mouse