A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane.
OBJECTIVE
To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's-eye analysis of surgically excised ILM.


METHODS
Laboratory investigation.


METHODS
The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy.


RESULTS
The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet.


CONCLUSIONS
This new bird's-eye-view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective.
Hisatomi
Toshio
T
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. hisatomi@med.kyushu-u.ac.jp
Enaida
Hiroshi
H
Sakamoto
Taiji
T
Kagimoto
Tadahisa
T
Ueno
Akifumi
A
Nakamura
Takao
T
Hata
Yasuaki
Y
Ishibashi
Tatsuro
T
D001485:Basement Membrane / Q000648:ultrastructure; D019773:Epiretinal Membrane / Q000473:pathology* / Q000601:surgery*; D005109:Extracellular Matrix / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D009457:Neuroglia / Q000378:metabolism / Q000648:ultrastructure; D009885:Ophthalmology / Q000379:methods; D014821:Vitrectomy
D005904:Glial Fibrillary Acidic Protein