Expression of CD44v6 gene in normal human peripheral blood.
OBJECTIVE
To investigate if CD44v6 could be used as a molecular marker of cancer progression and metastasis through the detection of CD44v6 gene expression in normal human peripheral blood.


METHODS
RNA was extracted from the peripheral blood mononuclear cells of 50 healthy donors, the expression of CD44v6 was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
CD44v6 mRNA was detected in 58% of healthy volunteers under the proper controls.


CONCLUSIONS
Our results suggest that the measurement of CD44v6 expression in peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
Song
Jian
J
Department of Pathology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China.
Zhang
Dong-Sheng
DS
Zheng
Jie
J
D015703:Antigens, CD / Q000097:blood / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000276:immunology; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics*; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood* / Q000235:genetics*; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology; D012313:RNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms
D015703:Antigens, CD; D014408:Biomarkers, Tumor; C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D012313:RNA