Transcription profiling of the metal-hyperaccumulator Thlaspi caerulescens (J. & C. PRESL).
Thlaspi caerulescens is a well-studied metal-hyperaccumulator of zinc, cadmium and nickel, belonging to the Brassicaceae family. Moreover it is one of the few hyperaccumulators that occur on different metalliferous soil types, as well as on nonmetalliferous soils. We are interested in the development of systems to improve phytoremediation of metal contaminated soils through improved metal-accumulation. About 1900 cDNAs isolated from T. caerulescens roots were hybridized with reverse transcribed RNA from zinc-treated T. caerulescens plants of two accessions originating from two different soil types. This comparative transcript profiling of T. caerulescens plants resulted in the identification of genes that are affected by heavy metals. The developed microarray proved to be an appropriate tool for a large scale analysis of gene expression in this metal-accumulator species.
Plessl
Markus
M
Institute of Biochemical Plant Pathology, GSF, National Research Center for Environment and Health, Ingolstaedter Landdstr, Germany. Plessl@gsf.de
Rigola
Diana
D
Hassinen
Viivi
V
Aarts
Mark G M
MG
Schat
Henk
H
D019607:Brassicaceae / Q000235:genetics* / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008670:Metals / Q000493:pharmacokinetics*; D009532:Nickel / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics; D014158:Transcription, Genetic; D015032:Zinc / Q000378:metabolism
D018744:DNA, Plant; D008670:Metals; D010940:Plant Proteins; D018749:RNA, Plant; D002104:Cadmium; D009532:Nickel; D015032:Zinc