[An in vitro study of cytotoxic and antineoplastic effect of Solanum nigrum L extract on U266].
OBJECTIVE
To study cytotoxicity and antineoplastic effect in vitro Solanum nigrum L extract on U266.


METHODS
U266 cells were cultured together with the extract of Solanum nigrum L. Cytotoxicity assay was tested by CCK-8. Cell cycle and apoptosis were determined using flow cytometry (FCM) analysis.


RESULTS
Extract of Solanum nigrum L showed strong cytotoxicity against U266 cells. The IC50 was about 117 mg/L. After exposure of U266 cells to the drug for 48 hours, the cell cycle distribution was changed compared with the controls. There was decrease of cells in the G0/G1 phase with increase of cells in the S phase and G2/M phase. Apoptosis of U266 cells could be shown with staining of Annexin V FITC/PI or TFAR19 testing through FCM. The proportion of apoptotic cells increased in parallel with the increase of the drug dosage.


CONCLUSIONS
Solanum nigrum L extract showed strong cytotoxicity effect on U266 cells. The antineoplastic effect of the drug can partly be ascribed to its apoptotic inducing effect.
Wang
Wei
W
Institute of hematology, Peking University People's Hospital, Beijing 100044, China.
Lu
Dao-pei
DP
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D003601:Cytotoxicity Tests, Immunologic; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D032324:Solanum nigrum / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal