Full verification of the liquid exclusion-adsorption chromatography theory using monolithic capillary columns.
Capillary electrochromatography (CEC) was used to separate alkyl phenol ethoxylates (APEs) as model diblock copolymers, with monolithic polymers as stationary phases. The order of elution indicate that the two polymer blocks follow distinct chromatographic modes: size-exclusion for the poly(oxyethylene) group and adsorption interaction for the alkyl part. Therefore, our experimental results were compared to the theory describing liquid exclusion-adsorption chromatography (LEAC). They were found in perfect agreement with the theory, which turned to be verified for the first time over the full range of polymer lengths.
Lacharme
Frédéric
F
Laboratoire d'Analyse Chimique par Reconnaissance Moléculaire (LACReM), ENSCPB, 16 Av Pey Berland, 33607 Pessac, France.
Lapeyre
Véronique
V
Ravaine
Valérie
V
D000098:Acetophenones / Q000302:isolation & purification; D000327:Adsorption; D002850:Chromatography, Gel / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000302:isolation & purification*; D011427:Propiophenones / Q000302:isolation & purification
D000098:Acetophenones; D011427:Propiophenones; D011092:Polyethylene Glycols; C038699:acetophenone