Prevention of cellular oxidative damage by an aqueous extract of Anoectochilus formosanus.
Anoectochilus formosanus (AF) is a popular folk medicine in Taiwan whose pharmacological effects have been characterized. In this work we investigated the antioxidant properties of an aqueous extract prepared from AF. The AF extract was capable of scavenging H2O2 in a dose-dependent manner. We induced oxidative stress in HL-60 cells, either by the addition of hydrogen peroxide (H2O2) or by the xanthine/xanthine oxidase reaction. Apoptosis caused by oxidative damage was displayed by DNA fragmentation on gel electrophoresis, and the apoptotic fraction was quantified with flow cytometry. The cell damage induced by oxidative stress was prevented by the plant extract in a concentration-dependent manner. Furthermore, the proteolytic cleavage of poly(ADP-ribose) polymerase during the apoptotic process was also inhibited by AF extract. Our results provide the basis for determining an AF extract to be an antioxidant.
Wang
Leng-Fang
LF
Department of Biochemistry, Taipei Medical University, 250 Wu Hsing St., Taipei, Taiwan, ROC.
Lin
Chun-Mao
CM
Shih
Chwen-Ming
CM
Chen
Hui-Ju
HJ
Su
Borcherng
B
Tseng
Cheng-Chuang
CC
Gau
Bao-Bih
BB
Cheng
Kur-Ta
KT
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D029595:Orchidaceae / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism
D000975:Antioxidants; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D019820:Xanthine; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D011065:Poly(ADP-ribose) Polymerases