Biosynthesis of pyrethrin I in seedlings of Chrysanthemum cinerariaefolium.
The biosynthetic pathway to natural pyrethrins in Chrysanthemum cinerariaefolium seedlings was studied using [1-13C]d-glucose as a precursor, with pyrethrin I isolated using HPLC from a leaf extract. The 13C NMR spectrum of pyrethrin I from the precursor-administered seedlings indicated that the acid moiety was biosynthesized from d-glucose via 2-C-methyl-d-erythritol 4-phosphate, whereas the alcohol moiety was possibly biosynthesized from linolenic acid.
Matsuda
Kazuhiko
K
Department of Applied Biological Chemistry, School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan. kmatsuda@nara.kindai.ac.jp
Kikuta
Yukio
Y
Haba
Atsushi
A
Nakayama
Koji
K
Katsuda
Yoshio
Y
Hatanaka
Akikazu
A
Komai
Koichiro
K
D001711:Biotransformation; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D011723:Chrysanthemum cinerariifolium / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011722:Pyrethrins / Q000302:isolation & purification / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*
D002247:Carbon Isotopes; D011722:Pyrethrins; D005947:Glucose; C009119:pyrethrin I