Characterization of Ty3-gypsy-like elements in Hibiscus syriacus.
Southern blot analysis revealed a ubiquitous distribu-tion and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Ji-Ung
JU
Jeung
Sung Ki
SK
Cho
Seung Jae
SJ
Lee
Jeong Sheop
JS
Shin
D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D031584:Hibiscus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
D018626:Retroelements