Single-molecule fluorescence spectroscopy of protein folding.
Single-molecule spectroscopy is an important new approach for studying the intrinsically heterogeneous process of protein folding. This Review illustrates how different single-molecule fluorescence techniques have improved our understanding of mechanistic aspects in protein folding, exemplified by a series of recent experiments on a small protein.
Schuler
Benjamin
B
Department of Biochemistry, University of Zürich, Winterthurerstr. 190, 8057 Zürich, Switzerland. schuler@bioc.unizh.ch
D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D011506:Proteins