Identification of lymphoma-associated antigens using SEREX.
Serological analysis of recombinant complementary deoxyribonucleic acid (cDNA) expression libraries (SEREX) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies that are naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognize bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full.
Liggins
Amanda P
AP
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, UK.
Guinn
Barbara A
BA
Banham
Alison H
AH
D000912:Antibodies, Neoplasm / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D001324:Autoantigens; D014408:Biomarkers, Tumor; D018076:DNA, Complementary