Increased erythromycin production by alginate as a medium ingredient or immobilization support in cultures of Saccharopolyspora erythraea.
Erythromycin production by Saccharopolyspora erythraea immobilized in 2% (w/v) calcium alginate or grown in medium containing 20 g sodium alginate/l inoculated with free cells was almost twice more than that of the control. S. erythraea did not consume alginate, agar, dextran, silicon antifoaming agent or cyclodextrin as a carbon source, although, all of these increased the production of erythromycin. Highest titer of erythromycin (2.3 times more than that of the control) was achieved in medium containing 1 g agar/l.
Microbial Biotechnology Laboratory, Department of Biology, Faculty of Science, University of Tehran, Tehran, Iran. jhamedi@khayam.ut.ac.ir
J
J
Hamedi
F
F
Khodagholi
A
A
Hassani-Nasab
D000362:Agar / Q000737:chemistry; D000464:Alginates / Q000737:chemistry / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000934:Antifoaming Agents / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized; D003470:Culture Media / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D004917:Erythromycin / Q000378:metabolism*; D005285:Fermentation; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D016930:Saccharopolyspora / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry
D000464:Alginates; D000900:Anti-Bacterial Agents; D000934:Antifoaming Agents; D003470:Culture Media; D003911:Dextrans; D006603:Hexuronic Acids; D011108:Polymers; D004917:Erythromycin; D020723:Glucuronic Acid; D000362:Agar; D012825:Silicon