Identification of a universal Group B streptococcus vaccine by multiple genome screen.
Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens.
Maione
Domenico
D
Chiron srl, Via Fiorentina 1, 53100 Siena, Italy.
Margarit
Immaculada
I
Rinaudo
Cira D
CD
Masignani
Vega
V
Mora
Marirosa
M
Scarselli
Maria
M
Tettelin
Hervé
H
Brettoni
Cecilia
C
Iacobini
Emilia T
ET
Rosini
Roberto
R
D'Agostino
Nunzio
N
Miorin
Lisa
L
Buccato
Scilla
S
Mariani
Massimo
M
Galli
Giuliano
G
Nogarotto
Renzo
R
Nardi-Dei
Vincenzo
V
Nardi Dei
Vincenzo
V
Vegni
Filipo
F
Fraser
Claire
C
Mancuso
Giuseppe
G
Teti
Giuseppe
G
Madoff
Lawrence C
LC
Paoletti
Lawrence C
LC
Rappuoli
Rino
R
Kasper
Dennis L
DL
Telford
John L
JL
Grandi
Guido
G
D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D019295:Computational Biology; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D007112:Immunity, Maternally-Acquired; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins; D010587:Phagocytosis; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D014611:Vaccination
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D009895:Opsonin Proteins; C414463:SIP protein, Streptococcus group B; D022541:Streptococcal Vaccines