Total flavones from Elsholtzia blanda reduce infarct size and improve heart function during acute myocardial infarction by inhibiting myocardial apoptosis in canines.
OBJECTIVE
The current study tests the hypothesis that inhibition of apoptotic cell death with total flavones from Elsholtzia blanda (TFEB), a traditional Chinese medicine, reduces infarct size and improves heart function during myocardial ischaemia induced by coronary occlusion in canines.


RESULTS
Myocardial apoptosis was detected by the TdT-mediated dUTP nick end labelling (TUNEL) method and DNA laddering. Infarct size of the left ventricle, serum level of CK-MB, haemodynamic parameters including rate of rise and decline of left ventricular pressure (+/- dp/dtmax), left ventricular systolic pressure (LVSP), main arterial pressure (MAP), coronary blood flow (CBF), coronary vascular resistance (CVR) were measured in this study. TUNEL positive cells were markedly reduced from 27.83 +/- 8.15% in the control dogs to 7.74 +/- 3.50% in the TFEB-treated ones (P < 0.01), consistent with the absence of DNA laddering. TFEB significantly reduced infarct size from 19.30 +/- 6.66% in the control dogs to 8.87 +/- 1.66% (P < 0.01), confirmed by lower serum CK-MB activity. TFEB significantly reduced MAP and CVR. The decrease in +/- dp/dtmax, LVSP and CBF also tended to be much smaller in the TFEB treated group.


CONCLUSIONS
These data suggest that the inhibitory effect of TFEB on apoptosis during coronary occlusion is associated with reduction in infarct size and improvement in heart function.
Ling
Haiyun
H
Department of Pharmacology and Toxicology, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, PR China.
Lou
Yija
Y
Wu
Honghai
H
Lou
Honggang
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003402:Creatine Kinase / Q000097:blood; D052279:Creatine Kinase, MB Form; D053938:DNA Fragmentation / Q000187:drug effects; D004285:Dogs; D004365:Drugs, Chinese Herbal; D047309:Flavones / Q000627:therapeutic use*; D006439:Hemodynamics / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D007527:Isoenzymes / Q000097:blood; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology*; D008517:Phytotherapy
D004365:Drugs, Chinese Herbal; D047309:Flavones; D007527:Isoenzymes; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form