Effect of methanol extract of Sorbus cortex in a rat model of L-NAME-induced atherosclerosis.
Chronic inhibition of nitric oxide (NO) synthesis by administration of high dose of N(G)-nitro-L-arginine methylester (L-NAME) induces vascular inflammation and subsequent atherosclerosis. We aimed to investigate whether the methanol extract of Sorbus commixta cortex (MSC) is able to prevent inflammatory process in a rat model of L-NAME-induced atherosclerosis. Chronic treatment with low or high doses of MSC prevented the L-NAME-induced increase in monocyte chemoattractant protein-1 (MCP-1) and nuclear factor-kappaB (NF-kappaB) p65 expressions as well as adhesion molecules including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in aorta. In addition, increased endothelin-1 (ET-1) and angiotensin converting enzyme (ACE) expressions and decreased endothelial cell NO synthase (ecNOS) expression in aorta from L-NAME treated group was reversed by treatment with MSC. From the histological examination, aortic segment from the L-NAME-treated rats revealed a thickening of intima and media, which was ameliorated by treatment with MSC. In conclusion, our results indicate that MSC can prevent atherosclerosis by inhibiting vascular over-expressions of vasoactive materials, pro-inflammatory transcription factor, and adhesion molecules and by augmenting ecNOS in chronic L-NAME-treated rat model.
Sohn
Eun Jin
EJ
Professional Graduate School of Oriental Medicine and Medicinal Resources Research Institute (MeRRI), Wonkwang University, Jeonbuk, Republic of Korea.
Kang
Dae Gill
DG
Choi
Deok Ho
DH
Lee
An Sook
AS
Mun
Yeon Ja
YJ
Woo
Won Hong
WH
Kim
Jin Sook
JS
Lee
Ho Sub
HS
D000818:Animals; D001161:Arteriosclerosis / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D017931:DNA Primers; D019332:Endothelin-1 / Q000235:genetics; D000432:Methanol / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000633:toxicity*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031994:Sorbus / Q000737:chemistry*; D051996:Transcription Factor RelA
D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D017931:DNA Primers; D019332:Endothelin-1; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D019331:NG-Nitroarginine Methyl Ester; D000432:Methanol