Assignment of sockeye salmon (Oncorhynchus nerka) to spawning sites using DNA markers.
Randomly amplified polymorphic DNA (RAPD) markers were used to assign individual adult sockeye salmon to their spawning sites using a genotype assignment test. Six primers were selected for use by screening bulked DNA samples for markers missing in fish from one or more of 5 sites in British Columbia or Alaska. Of 73 markers scored, 54 showed variation between or within sites among the sampled fish. Thirty-seven of the variable markers were not detected in any fish from one or more sites; 18 variable markers were detected in all fish from one or more other sites. Thus 25% of markers scored were found in all fish of some sites and in no fish of some other sites. An assignment test placed all 70 fish tested into their correct populations. Principal coordinate analysis of genetic variation produced clusters of fish corresponding to each sampling site. No sex-specific RAPD markers were detected among more than 1300 screened.
Department of Molecular Biology and Biochemistry, Simon Fraser University, BC V5A 1S6, Canada.
Graham E
GE
Corley-Smith
Liv
L
Wennerberg
Joy A
JA
Schembri
Chinten J
CJ
Lim
Karen L
KL
Cooper
Bruce P
BP
Brandhorst
D000413:Alaska; D000818:Animals; D001955:British Columbia; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005820:Genetic Testing; D005843:Geography; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011157:Population Dynamics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012474:Salmon / Q000145:classification / Q000235:genetics* / Q000502:physiology; D012726:Sexual Behavior, Animal
D005819:Genetic Markers