Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia pastoris.
The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZalphaA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P. pastoris X-33, and EntP was heterologously secreted from the recombinant P. pastoris X-33t1 derivative at a higher production and antagonistic activity than from E. faecium P13.
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Jorge
J
Gutiérrez
Raquel
R
Criado
María
M
Martín
Carmen
C
Herranz
Luis M
LM
Cintas
Pablo E
PE
Hernández
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010843:Pichia / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D011994:Recombinant Proteins; C417717:enterocin P