[Cefotaxime-resistant shiga toxin-producing Escherichia coli O26 : H11 isolated from a patient with diarrhea].
A shiga toxin-producing Escherichia coli (STEC) O26 strain resistant to cefotaxime (CTX) and cefpodoxime (but not ceftazidime) was isolated from the faecal sample of a 17-year-old outpatient with diarrhea. The double disk synergy test, twin test, polymerase chain reaction and sequence analysis confirmed that the strain produced CTX-M-3 type extended-spectrum beta-lactamase (ESBL). Conjugation experiment results suggested that the CTX resistance in this strain was determined by an approximately 85kbp plasmid that was readily transferable to a susceptible recipient E. coli strain. This is the first report from Japan of CTX-M-3type ESBL-producing STEC O26.
Division of Clinical Microbiology, Saitama Institute of Public Health.
Marina
M
Kon
Takayuki
T
Kurazono
Mariko
M
Ohshima
Masanori
M
Yamaguchi
Koji
K
Morita
Noboru
N
Watanabe
Masato
M
Kanamori
Shigeru
S
Matsushita
D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology*; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D006801:Humans; D022621:Shiga Toxin / Q000096:biosynthesis*; D018440:beta-Lactam Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D022621:Shiga Toxin; D002439:Cefotaxime