[The effect of ribozyme specially cleaving per1 mRNA on c-fos mRNA and its expression in hippocampus of morphine addicted mice].
OBJECTIVE
To study the change of c-fos mRNA and protein in hippocampus of morphine addicted mice after injected with ribozyme specially cleaving per1 mRNA.


METHODS
The recombined plasmid pcDNA 3.1-per1RZ DNA was injected into the ventricles of morphine addicted mice to transcript the corresponding ribozyme which cleaves per1 mRNA particularly. And then, the brains of mice were fixed by perfusion. The level of c-fos mRNA was assayed by in situ hybridization and c-fos protein was detected by immunohistochemical staining.


RESULTS
The level of c-fos mRNA and protein decreased after injection of the recombined plasmid pcDNA 3.1-per1RZ DNA expressing the ribozyme cleaving per1 mRNA.


CONCLUSIONS
The ribozyme specially cleaving per1 mRNA has potential function in inhibiting the transcription and expression of c-fos and blocking the morphine addiction.
Department of Biochemistry and Molecular Biology, School of Basic Medicine and Forensic, Sichuan University, Chengdu Sichuan, China.
Ying-hui
YH
Liu
Tao
T
Peng
Li-guo
LG
Chen
Jing
J
Xiao
Zhen-rong
ZR
Wang
Yan-you
YY
Liu
D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009021:Morphine Dependence / Q000503:physiopathology; D009687:Nuclear Proteins / Q000494:pharmacology*; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger