Expression of 15-lipoxygenase-1 in human nasal epithelium: its implication in mucociliary differentiation.
15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here, we investigated the relation between 15-LO-1 expression and the differentiation of human nasal epithelium. In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in normal human nasal epithelial cell time-dependently increased, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 at 1 ng/ml concentration time-dependently induced 15-LO-1 expression. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In murine nasal mucosa, the expression of leukocyte type-12-LO, a functional equivalent of 15-LO-1, reduced after postnatal day 7. Our findings suggest that 15-LO-1 is related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of human nasal epithelium.
Kim
Kyung-Su
KS
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea. ydrhinol@yumc.yonsei.ac.kr
Chun
Hee-Sun
HS
Yoon
Joo-Heon
JH
Lee
Jeung Gweon
JG
Lee
Joo-Hwan
JH
Yoo
Jong-Bum
JB
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D002923:Cilia / Q000201:enzymology; D003470:Culture Media; D015870:Gene Expression; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D051379:Mice; D009077:Mucins / Q000235:genetics; D009093:Mucus / Q000166:cytology; D009297:Nasal Mucosa / Q000166:cytology / Q000201:enzymology*; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation
D003470:Culture Media; D005819:Genetic Markers; C105059:MUC8 protein, human; D009077:Mucins; D015847:Interleukin-4; D014212:Tretinoin; D001093:Arachidonate 15-Lipoxygenase