(+)Insert smooth muscle myosin heavy chain (SM-B) isoform expression in human tissues.
Two smooth muscle myosin heavy chain isoforms differ in their amino terminus by the presence [(+)insert] or absence [(-)insert] of a seven-amino acid insert. Animal studies show that the (+)insert isoform is predominantly expressed in rapidly contracting phasic muscle and the (-)insert isoform is mostly found in slowly contracting tonic muscle. The expression of the (+)insert isoform has never been demonstrated in human smooth muscle. We hypothesized that the (+)insert isoform is present in humans and that its expression is commensurate with the organ's functional requirements. We report, for the first time, the sequence of the human (+)insert isoform and quantification of its expression by real-time PCR and Western blot analysis in a panel of human organs. The (+)insert isoform mRNA and protein expression levels are significantly greater in small intestine compared with all organs studied except for trachea and are significantly greater in trachea compared with uterus and aorta. To assess the functional significance of this differential myosin isoform expression between organs, we measured the rate of actin filament movement (nu(max)) when propelled by myosin purified from rat organs, because the rat and human inserts are identical and their remaining sequences show 93% identity. nu(max) exhibits a rank correlation from the most tonic to the most phasic organ. The selective expression of the (+)insert isoform observed among human organs suggests that it is an important determinant of tissue shortening velocity. A differential expression of the (+)insert isoform could also account for altered contractile properties observed in human pathology.
Léguillette
Renaud
R
Meakins-Christie Laboratories, McGill University, 3626 St-Urbain St., Montréal, QC, Canada, H2X 2P2.
Gil
Fulvio R
FR
Zitouni
Nedjma
N
Lajoie-Kadoch
Stéphane
S
Sobieszek
Apolinary
A
Lauzon
Anne-Marie
AM
D000595:Amino Acid Sequence; D000818:Animals; D015870:Gene Expression; D006801:Humans; D009130:Muscle, Smooth / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000096:biosynthesis*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D024745:Smooth Muscle Myosins / Q000096:biosynthesis*; D014018:Tissue Distribution
D020033:Protein Isoforms; D012333:RNA, Messenger; D024745:Smooth Muscle Myosins; D018995:Myosin Heavy Chains