[Identification of proteins interacting with adaptor protein Bam32].
OBJECTIVE
To study the role of adaptor protein Bam32 in B cell antigen receptor (BCR) signaling cascades.


METHODS
Using full length Bam32 as bait, yeast two-hybrid technique was used to screen the protein that could interact with Bam32. The interaction was further confirmed by co-transfection of 293T cells and coimmunoprecipitation.


RESULTS
Protein tyrosine kinase Lyn was one of the strong positive clones identified by the yeast two-hybrid screening. This interaction was further confirmed in 293T cells by co-transfection and coimmunoprecipitation. By using specific anti-phosphotyrosine antibody, it was found that Bam32 could be phosphorylated by Lyn.


CONCLUSIONS
The interaction of Bam32 with Lyn leads to Bam32 phosphorylation, which might play an important role in activating downstream signaling molecules.
Cheng
Xiao-xing
XX
Department of Microbiology, Third Military Medical University, Chongqing 400038, China. xiaoxing@mail.tmmu.com.cn
Jian
Rui
R
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006801:Humans; D047468:Immunoprecipitation; D008074:Lipoproteins / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation; D011485:Protein Binding; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D020798:Two-Hybrid System Techniques; D019061:src-Family Kinases / Q000032:analysis / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C403524:Dapp1 protein, mouse; D008074:Lipoproteins; D011947:Receptors, Antigen, B-Cell; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases