Degradation and drug release of phosphate glass/polycaprolactone biological composites for hard-tissue regeneration.
Phosphate-based glass (P-glass) and poly(epsilon-caprolactone) (PCL) composites were fabricated in a sheet form by solvent extraction and thermal pressing methods, and the antibiotic drug Vancomycin was loaded within the composites for use as a hard-tissue regenerative. The degradation and drug-release rate of the composites in vitro were tailored by modifying the glass composition: 0.45 P(2)O(5)-x CaO-(0.55-x)Na(2)O, where x=0.2, 0.3, 0.4, and 0.5. Compared to pure PCL, all the P-glass/PCL composites degraded to a higher degree, and the composite with lower-CaO glass showed a higher material loss. This was attributed mainly to the dissolution of the glass component. The glass dissolution also increased the degradation of PCL component in the composites. The Vancomycin release from the composites was strongly dependent on the glass composition. Drug release in pure PCL was initially abrupt and flattened out over a prolonged period. However, glass/PCL composites (particularly in the glass containing higher-CaO) exhibited a reduced initial burst and a higher release rate later. Preliminary cell tests on the extracts from the glass/PCL composites showed favorable cell proliferation, but the level was dependent on the ionic concentration of the extracts. The cell proliferation on the diluted extracts from the composite with higher-CaO glass was significantly higher than that on the blank culture dish. These observations confirmed that the P-glass/PCL composites are potentially applicable for use as hard-tissue regeneration and wound-healing materials because of their controlled degradation and drug-release profile as well as enhanced cell viability.
School of Materials Science and Engineering, Seoul National University, Seoul, 151-742, Korea. hwkim@snu.ac.kr
Hae-Won
HW
Kim
Eun-Jung
EJ
Lee
In-Kook
IK
Jun
Hyoun-Ee
HE
Kim
Jonathan C
JC
Knowles
D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration; D002118:Calcium / Q000032:analysis; D002460:Cell Line; D002470:Cell Survival; D003188:Composite Resins / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D016503:Drug Delivery Systems / Q000295:instrumentation*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010710:Phosphates / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing
D001672:Biocompatible Materials; D003188:Composite Resins; D003470:Culture Media; D010710:Phosphates; D011091:Polyesters; C016240:polycaprolactone; D002118:Calcium