[Establishment of Genic Male Sterile Lines of Brassica napus by Wide Cross and Their Cytology and Morphology.].
One and three male sterile plants were found in the progenies of the intergeneric hybrids by crossing Brassica napus and Brassica juncea to Orychophragmus violaceus, respectively. After interspecific cross and full-sib crosses, four genic male sterile lines were obtained. The results of cytological observations indicated that somatic chromosome number was 38 in all types of sterile lines, which were considered as the type of B. napus. Most of pollen mother cells (PMC) in all sterile plants were observed normally for chromosome behaviour. But abnormal phenomena of chromosome, which present for chromosome lagging and bridge, were still found at each stage of meiosis in these sterile lines. These lines belonged to complete sterile type. There were distinct differences between sterile plants and normal plants in the flower morphology. Bud death at different degrees was found on anthotaxies in sterile plants. Studies on the flower growth showed that the growths of the pistils in sterile plants were gradually accelerated along with pollen sterility. At the same time there were two slowly growth stages of the pistils growth in normal plants. The prospects of these sterile lines were discussed in this paper.
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University.
Xing-Wu
XW
Lin
Jian-Guo
JG
Wu
Chun-Hai
CH
Shi
D029688:Brassica napus; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D004171:Diploidy; D006824:Hybridization, Genetic; D008540:Meiosis; D011058:Pollen / Q000235:genetics