Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor.
In this work, the results of a method based on an electrochemical biosensor to detect DNA damage in vitro for the evaluation of the antioxidant properties of plant extracts are reported. The biosensor consisted of a dsDNA immobilized on a screen-printed electrode surface (SPE). DNA damage was promoted by the generation of the *OH radicals via Fenton-type reaction. The interaction of the radical species with immobilised DNA in the absence and presence of antioxidants was evaluated by means of changes in the guanine oxidation peak obtained by square wave voltammetry. The results demonstrated that the DNA-based biosensor is suitable as a rapid screening test for the evaluation of antioxidant properties of samples.
Instituto de Química, Universidade de Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
Lucilene Dornelles
LD
Mello
Silvia
S
Hernandez
Giovanna
G
Marrazza
Marco
M
Mascini
Lauro Tatsuo
LT
Kubota
D000975:Antioxidants / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004249:DNA Damage; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010936:Plant Extracts / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D004247:DNA; C427203:calf thymus DNA