[Mangrove soil community analysis using DGGE of 16S rDNA V3 fragment polymerase chain reaction products].
The 16S rDNA V3 fragment polymerase chain reaction products amplified from mangrove soil-DNA extracts were analyzed by denaturing gradient gel electrophoresis (DGGE) followed by clone technology. Regional factors and mangrove species were found to influence the soil microbe community. Two DNA slices separated on denaturing gradient gel from Sonneratia alba soil sample were recovered and the recovered DNA were used to establish two clone libraries. Some clones were sequenced and each sequence was compared with all nucleotide sequences in GenBank database. Most cloned sequences were found not in GenBank database. These results suggest that DGGE followed by clone technique is a practicable protocol to research the complex community of soil microbe.
Wang
Yue-kun
YK
Institute of Tropical Biological Sciences and Biotechnology, Chinese Academy of Tropical Agrocultural Science, Haikou 571101, China. xwangyk@126.com
Hong
Kui
K
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S