Self-assembled monolayers of alpha,omega-diphosphonic acids on Ti enable complete or spatially controlled surface derivatization.
Alpha,omega-diphosphonic acids self-assemble on the native oxide surfaces of Ti or Ti-6Al-4V. Heating gives strongly bonded phosphonate monolayers. Infrared and X-ray spectroscopic and water contact angle data show that the films are bonded to the surface by one phosphonate unit; the other remains a phosphonic acid. Surface loadings were measured by quartz crystal microbalance procedures. Mechanical shear strengths for the films were also measured; these do not correlate simply with surface loadings. Films formed from 1,12-diphosphonododecane were treated with zirconium tetra(tert-butoxide) to give surface Zr complex species; derivatives of these surface complexes are stable to hydrolysis under physiological conditions and are mechanically strong. The complexation reaction can be accomplished over the entire surface; alternatively, dropwise application of the alkoxide to the surface enables spatial control of deposition. The cell attractive peptide derivative RGDC can be bound to these surface Zr alkoxide complexes through (maleimido)-alkylcarboxylate intermediates. Surfaces modified with RGDC were shown to be effective for osteoblast binding and proliferation.
Department of Chemistry, Princeton University, Princeton, New Jersey 08544-1009, USA.
Michael P
MP
Danahy
Michael J
MJ
Avaltroni
Kim S
KS
Midwood
Jean E
JE
Schwarzbauer
Jeffrey
J
Schwartz
D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D006868:Hydrolysis; D008422:Materials Testing; D015394:Molecular Structure; D063065:Organophosphonates / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D014025:Titanium / Q000737:chemistry*
D063065:Organophosphonates; D014025:Titanium