A low-protein diet alters rat behavior and neurotransmission in normothermic and hyperthermic environments.
Dietary protein contains amino acids used in the brain for synthesis of neurotransmitters. Although information on pre- and post-natal exposure to low-protein diets in rodents is available, little is known about effects of such diets on adult animals. Therefore, the behavioral and neurochemical consequences of exposure to a brief (11 days), low (4%)-protein diet in animals exposed to normothermic and hyperthermic test conditions were examined. In separate groups of animals, the Porsolt Swim test and elevated plus maze were administered. These tasks are sensitive to nutritional and/or environmental manipulations. In other groups of rats exposed to the same dietary and environmental conditions, dopamine, norepinephrine, epinephrine, and serotonin in the striatum were assessed using microdialysis. In the Porsolt swim test, which assesses coping behavior, performance was impaired under normothermic and hyperthermic conditions in animals on the low-protein diet. Performance on the plus maze, a measure of exploration and anxiety, was altered in the hyperthermic condition by low protein, with the diet increasing exploration. Microdialysis detected increased norepinephrine in the striatum of hyperthermic animals on the low-protein diet. This study demonstrates that changes in stress-related behaviors of adult animals occur following brief exposure to low-protein diets.
Military Nutrition Division, United States Army Research Institute of Environmental Medicine, Natick, MA 01760-5007, USA. harris.lieberman@na.amedd.army.mil
Harris R
HR
Lieberman
Sylva K
SK
Yeghiayan
Timothy J
TJ
Maher
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001923:Brain Chemistry / Q000502:physiology*; D018753:Diet, Protein-Restricted / Q000379:methods*; D005334:Fever / Q000503:physiopathology*; D046353:Immobility Response, Tonic / Q000502:physiology; D008297:Male; D018782:Maze Learning; D017551:Microdialysis / Q000379:methods; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012016:Reference Values; D013550:Swimming; D013997:Time Factors
D018377:Neurotransmitter Agents