Structure of human PNP complexed with ligands.
Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.
Canduri
Fernanda
F
Programa de Pós-Graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
Silva
Rafael Guimarães
RG
dos Santos
Denis Marangoni
DM
Palma
Mário Sérgio
MS
Basso
Luiz Augusto
LA
Santos
Diógenes Santiago
DS
de Azevedo
Walter Filgueira
WF
D001375:Azaguanine / Q000737:chemistry; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006147:Guanine / Q000737:chemistry; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D007288:Inosine / Q000737:chemistry; D008024:Ligands; D011684:Purine Nucleosides; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011744:Pyrimidinones / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry
D008024:Ligands; D011684:Purine Nucleosides; D011744:Pyrimidinones; D011758:Pyrroles; D006151:Guanosine; C113101:forodesine; D007288:Inosine; D006147:Guanine; D011683:Purine-Nucleoside Phosphorylase; D001375:Azaguanine