Isolation and structure determination of bioactive isoflavones from callus culture of Dipteryx odorata.
Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4',6-dimethoxyisoflavone and 3',7-dihydroxy-4',6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4',6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4',6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.
Januário
Ana Helena
AH
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP, São Paulo, Brazil. ajanuari@unaerp.br
Lourenço
Miriam Verginia
MV
Domézio
Lilian Aparecida
LA
Pietro
Rosemeire Cristina Linhari Rodrigues
RC
Castilho
Marcelo Santos
MS
Tomazela
Daniela Maria
DM
da Silva
Maria Fátima das Graças Fernandes
MF
Vieira
Paulo Cezar
PC
Fernandes
João Batista
JB
França
Suzelei de Castro
Sde C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D046914:Dipteryx / Q000737:chemistry*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D014349:Trypanosoma cruzi / Q000201:enzymology
D007529:Isoflavones; D011994:Recombinant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases