Characterization of Ty3-gypsy-like elements in Hibiscus syriacus.
Southern blot analysis revealed a ubiquitous distribu-tion and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
Jeung
Ji-Ung
JU
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Cho
Sung Ki
SK
Lee
Seung Jae
SJ
Shin
Jeong Sheop
JS
D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D031584:Hibiscus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
D018626:Retroelements