Extracorporeal whole blood immunoadsorption of autoimmune myasthenia gravis by cellulose tryptophan adsorbent.
Whole blood immunoadsorption (WBIA) system, using an adsorbent to remove pathogenic antibodies of myasthenia gravis (MG), was studied. Cellulose-tryptophan adsorbent was synthesized and its adsorption capacity of binding with acetylcholine receptor in the plasma of MG patient was evaluated. Experimental autoimmune myasthenia gravis (EAMG) rabbits were induced by Ta183-200 peptide. The rabbits underwent extracorporeal whole blood adsorption for 2 h. Results showed no significant damages on blood cells and no changes in the concentration of electrolytes. Total protein decreased by 12.0% (P < 0.05), and globulin protein decreased 23.9 +/- 5.6% (P < 0.05). The mean overall removal of antibodies against Ta183-200 was 41.12%. The percentage of decrement of compound muscle action potential in 3, 5, 10Hz of EAMG rabbits all dropped down after the treatment. In conclusion, the adsorbent is biocompatible, was safe for whole blood immunoadsorption, and can remove antibodies in an MG patient effectively. Whole blood immunoadsorption improved clinical manifestation and neuromuscular function of the EAMG rabbits.
Neurology Department, Tianjin Medical University General Hospital, PR China.
Li
L
Yang
Yan
Y
Cheng
Wei-Rong
WR
Yan
Yao-Ting
YT
Yu
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D002482:Cellulose / Q000737:chemistry / Q000627:therapeutic use; D005260:Female; D006440:Hemofiltration / Q000379:methods*; D007164:Immunosorbents / Q000737:chemistry / Q000627:therapeutic use*; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry / Q000627:therapeutic use
D001323:Autoantibodies; D007164:Immunosorbents; D011950:Receptors, Cholinergic; D014364:Tryptophan; D002482:Cellulose