Hydroxysafflor yellow A protects rat brains against ischemia-reperfusion injury by antioxidant action.
The present study was conducted to investigate whether hydroxysafflor yellow A (HSYA) has a protective effect on brain injury after focal cerebral ischemia reperfusion, and to determine the possible mechanism. Behavioral tests were used to evaluate the damage to central nervous system. The infarct volume of brain was assessed in brain slices stained with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Adult male Wistar rats were subjected to 2h of middle cerebral artery occlusion and 24h of reperfusion. Spectrophotometric assay was used to determine the content of malondialdehyde (MDA), and the activity of total antioxidative capability (T-AOC) and superoxide dismutase (SOD). The results showed that treatment with HSYA (2, 4, 8 mg/kg, i.v.) significantly decreased neurological deficit scores and reduced the percentage of infarction in the ipsilateral hemisphere compared with the model group. At the same time, HSYA treatment significantly attenuated the elevation of MDA content, the decrease in SOD activity, and the T-AOC in the ipsilateral hemisphere and serum. All of these findings suggest that HSYA might provide neuroprotection against cerebral ischemia/reperfusion injury through its antioxidant action.
Wei
Xinbing
X
Department of Pharmacology, School of Medicine, Shandong University, Wenhua West Road 44#, Jinan, Shandong 250012, PR China.
Liu
Huiqing
H
Sun
Xia
X
Fu
Fenghua
F
Zhang
Xiumei
X
Wang
Jin
J
An
Jie
J
Ding
Hua
H
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D008315:Malondialdehyde / Q000378:metabolism; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011809:Quinones / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000378:metabolism; D016896:Treatment Outcome
D000975:Antioxidants; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D011809:Quinones; C085278:hydroxysafflor yellow A; D008315:Malondialdehyde; D002599:Chalcone; D013482:Superoxide Dismutase