Expanding the conformational pool of cis-beta-sugar amino acid: accommodation of beta-hGly motif in robust 14-helix.
Tendencies of forming stable helices of heterooligomers composed of alternating rigid cis-beta-sugar amino acid and flexible beta-hGly motifs have been investigated, using a combination of molecular mechanics, CD, FT-IR, and NMR techniques. The results show that the solution structures of these oligomers exist as robust right-handed 14-helices. Here, we examine the role of conformationally rigid cis-beta-sugar amino acid in preorganizing the conformation of beta-hGly to form the 14-helix. Our findings also show that a right-handed 14-helix can be formed with as few as four properly sequenced heterogeneous residues. These results represent the expansion of the conformational pool of sugar amino acid in the design of well-folded 14-helices, which can be used to develop beta-peptides endowed with biological activity.
Division of Organic Chemical Sciences, Indian Institute of Chemical Technology, Hyderabad 500 007, India. srivaric@iict.res.in
Srivari
S
Chandrasekhar
Marepally Srinivasa
MS
Reddy
Bathini Nagendra
BN
Babu
Bharatam
B
Jagadeesh
Anabathula
A
Prabhakar
Bulusu
B
Jagannadh
D002241:Carbohydrates / Q000737:chemistry*; D002942:Circular Dichroism; D005998:Glycine / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D017433:Protein Structure, Secondary
D002241:Carbohydrates; D005998:Glycine