Water intake during the development of renal hypertension (2K-1C) in mice.
For both practical and methodological reasons, mice have been the most widely employed species for development of transgenic and gene knockin and knockout animals. However, basic behavioral and physiology control and regulatory mechanisms in mice are not well characterized. To broaden our understanding of the processes maintaining body fluid and blood pressure homeostasis in the mouse, the objectives of this study were to evaluate voluntary water, and sodium intakes during the development of renal hypertension and to examine the relationship between hypertension and the quantities of water and salt ingested. In male, C57BL/6J mice, two-kidney, one-clip renal hypertension (2K-1C) was induced, and water and 1.8% NaCl intakes were monitored for 2 weeks. At the end of this period, all animals received arterial catheters for direct recording of blood pressure. The mice that received renal artery clips were sorted into hypertensive (152+/-4 mm Hg) and normotensive (122+/-2 mm Hg) groups and were compared to control (117+/-4 mm Hg) animals that underwent a sham renal clipping procedure. Hypertensive 2K-1C animals had significantly elevated water intake compared to control animals. On most of the postsurgical days, the normotensive 2K-1C animals did not display increased water intake in comparison to the control group. No significant effect was detected for 1.8% saline intake between any of the pairs of groups. In summary, the reduction of blood flow to a single kidney in the 2K-1C model of renal hypertension induces high blood pressure accompanied by sustained hyperdipsia in the mouse.
Department of Psychology, University of Iowa, Iowa City, IA 52242, USA.
Claudia M
CM
Santos
Ralph F
RF
Johnson
Terry G
TG
Beltz
Robert L
RL
Thunhorst
Alan Kim
AK
Johnson
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D004326:Drinking / Q000502:physiology*; D006977:Hypertension, Renal / Q000503:physiopathology*; D006978:Hypertension, Renovascular / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage; D012964:Sodium / Q000378:metabolism; D013997:Time Factors
D012462:Saline Solution, Hypertonic; D012964:Sodium