Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.
Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Higashimura-cho, Hiroshima, Japan.
Takenori
T
Satomura
Daisuke
D
Shimura
Kei
K
Asai
Yoshito
Y
Sadaie
Kazutake
K
Hirooka
Yasutaro
Y
Fujita
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D017930:Genes, Reporter; D005838:Genotype; D005972:Glutaminase / Q000235:genetics / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D000071677:Histidine Kinase; D007700:Kinetics; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D015534:Trans-Activators; C037853:glutamine transport proteins; D005973:Glutamine; D011494:Protein Kinases; D000071677:Histidine Kinase; D001616:beta-Galactosidase; D005972:Glutaminase