Two primer pairs to detect OTA producers by PCR method.
Fungi contaminating foods and feeds may produce many mycotoxins including ochratoxin A (OTA). Early and rapid detection of potential OTA producing fungi is important to reduce the negative impacts of OTA. In this study, two PCR specific primer pairs, AoLC35-12L/AoLC35-12R and AoOTAL/AoOTAR, were designed from a DNA sequence of a polyketide synthase gene in Aspergillus ochraceus NRRL 3174. On 14 different fungi tested by PCR, AoLC35-12L/AoLC35-12R amplified a unique band from either OTA or citrinin producers while AoOTAL/AoOTAR amplified one PCR product only from A. ochraceus. So these primers could be used to detect both OTA and citrinin producing fungi (AoLC35-12L/AoLC35-12R) or only A. ochraceus (AoOTAL/AoOTAR) from foodstuffs using PCR method.
Equipe Génie des Systèmes Microbiens, Laboratoire de Génie Chimique UMR5503 (CNRS/INPT/UPS), Ecole Nationale Supérieure Agronomique de Toulouse, Institut National Polytechnique de Toulouse, 1, avenue de l'Agrobiopôle, BP107, 31326 Castanet Tolosan, France.
Huy Phong
HP
Dao
Florence
F
Mathieu
Ahmed
A
Lebrihi
D000595:Amino Acid Sequence; D001235:Aspergillus ochraceus; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D009793:Ochratoxins / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment
D017931:DNA Primers; D004271:DNA, Fungal; D009793:Ochratoxins; C025589:ochratoxin A