PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes.
PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity in mediating transcription of different interferon-responsive genes. The selectivity by PBAF requires a novel subunit, BAF200, but not the previously described PBAF-specificity subunit, BAF180 (Polybromo). Our study provides in vivo evidence that PBAF and BAF regulate expression of distinct genes, and suggests that BAF200 plays a key role in PBAF function.
Laboratory of Genetics, National Institute on Aging, Baltimore, Maryland 21224, USA.
Zhijiang
Z
Yan
Kairong
K
Cui
Darryl M
DM
Murray
Chen
C
Ling
Yutong
Y
Xue
Amy
A
Gerstein
Ramon
R
Parsons
Keji
K
Zhao
Weidong
W
Wang
D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D021122:Protein Subunits; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D007370:Interferon Type I; D046912:Multiprotein Complexes; D021122:Protein Subunits; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors