Degradation of P(3HB) and P(3HB-co-3HV) in biological media.
The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with beta-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38 degrees C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance.
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russia.
E I
EI
Shishatskaya
T G
TG
Volova
S A
SA
Gordeev
A P
AP
Puzyr
D000818:Animals; D001673:Biodegradation, Environmental; D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D000094122:Polyhydroxybutyrates
D002021:Buffers; D006885:Hydroxybutyrates; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate