Impairment of VLDL secretion by medium-chain fatty acids in chicken primary hepatocytes is affected by the chain length.
To determine the effect of the chain length of medium-chain fatty acids (MCFAs) on VLDL secretion, the media of chicken hepatocyte cultures were supplemented with hexanoate (6:0), octanoate (8:0), decanoate (10:0), or dodecanoate (12:0). The supplementation of palmitate (16:0) or bovine serum albumin (BSA) alone in media was used as the positive control or the control, respectively. Palmitate significantly increased intracellular triacylglycerol (TG) accumulation and VLDL-TG, -cholesterol, and -apolipoprotein (apo)B secretion. On the other hand, the addition of hexanoate did not affect these variables relative to control cultures supplemented with BSA alone, whereas octanoate, decanoate, and dodecanoate decreased apoB secretion from the chicken hepatocytes. ApoB secretion from hepatocytes cultured with 1.0 mmol/L MCFA, in particular decanoate and dodecanoate, in the presence of 0.2 mmol/L palmitate was significantly lower than that obtained with 0.2 mmol/L palmitate alone. Decanoate at 0.25-1.0 mmol/L dose dependently reduced apoB mRNA expression compared with the control (BSA alone). The levels of 3-hydroxy-3-metylglutaryl-CoA reductase and apoA-I mRNA were significantly lower in cultures supplemented with hexanoate, octanoate, and decanoate than in cultures with dodecanoate and palmitate. These changes did not correspond to the reduction in VLDL-apoB secretion. We suggest that MCFAs with different chain lengths differentially affect apoB secretion and mRNA expression, with decanoate being the most effective at decreasing VLDL-apoB secretion by regulating apoB mRNA expression at the transcriptional level.
Laboratory of Animal Nutrition, Graduate School of Agricultural Science, Tohoku University, Japan.kan@bios.tohoku.ac.jp
Kan
K
Sato
Yositake
Y
Cho
Shizuko
S
Tachibana
Tomoyuki
T
Chiba
Wolfgang J
WJ
Schneider
Yukio
Y
Akiba
D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics / Q000378:metabolism; D002208:Caproates / Q000494:pharmacology; D002478:Cells, Cultured; D002645:Chickens; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D008079:Lipoproteins, VLDL / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000378:metabolism
D001055:Apolipoproteins B; D002208:Caproates; D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; D014280:Triglycerides; C037652:hexanoic acid