Opiate regulation of IL-1beta and TNF-alpha in cultured human articular chondrocytes.
In order to investigate if beta-endorphins anti-inflammatory effect in cartilage-damaging states is mediated via tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), we examined its influence on these two cytokines in vitro. Human articular chondrocytes were obtained from patients undergoing total knee arthroplasty and stimulated with beta-endorphin (60-6000 ng/ml). Protein levels of TNF-alpha and IL-1 beta were measured by ELISA in supernatants from articular chondrocyte cultures. beta-Endorphin significantly increased the levels of IL-1 beta for all concentrations used after 15 min incubation, and when stimulated with 600 and 6000 ng/ml after 24 h incubation. The opioid-induced increase in IL-1 beta was blocked by naltrexone in the group tested. TNF-alpha expression was also significantly stimulated by 60 and 600 ng/ml beta-endorphin after 15 min, an effect blocked by naltrexone in the group tested. These findings indicate that the mechanism of beta-endorphins anti-inflammatory influence in cartilage-damaging states is not apparently mediated via these two cytokines modulation.
Department of Orthopaedics, University Hospital of Northern-Norway, 9038 Tromsø, Norway. nenad.andjelkov@fagmed.uit.no
Nenad
N
Andjelkov
Jan
J
Elvenes
James
J
Martin
Oddmund
O
Johansen
D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D009294:Narcotics / Q000008:administration & dosage; D010100:Oxygen / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D001615:beta-Endorphin / Q000008:administration & dosage*
D007375:Interleukin-1; D009294:Narcotics; D014409:Tumor Necrosis Factor-alpha; D001615:beta-Endorphin; D010100:Oxygen