Effect of a cyclohexenonic long-chain fatty alcohol on calcium mobilization.
Cyclohexenonic long-chain fatty alcohols constitute a family of synthetic compounds with trophic, secretagogue and antioxidant properties. Despite their multiple biological actions in neuronal and non-neuronal tissues, the intracellular mechanisms underlying CFA activity remain unknown. In the present study, we show that 3-(15-hydroxypentadecyl)-2,4,4-trimethyl-2-cyclohexen-1-one (tCFA15) directly mobilizes Ca(2+) in the pituitary neural lobe synaptosomes and in primary sensory neurons from dorsal root ganglia. This effect is dependent on the presence of extracellular Ca(2+), but does not involve transmembrane voltage-operated calcium channels. Using a combination of pharmacological agents that block or deplete intracellular Ca(2+) stores, our results suggest the implication of a calcium induced-calcium release mechanism evoked by tCFA15-induced Ca(2+) influx. To our knowledge, these findings constitute the first attempt towards the comprehension of the biological actions of cyclohexenonic long-chain fatty alcohols at a molecular level.
Jover
Emmanuel
E
Laboratoire de Neurophysiologie Cellulaire et Intégrée, UMR CNRS 7519, Neurotransmission et sécrétion neuroendocrine, France. jover@neurochem.u-strasbg.fr
Gonzalez de Aguilar
Jose-Luis
JL
Luu
Bang
B
Lutz-Bucher
Bernadette
B
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003512:Cyclohexanones / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
C420963:3-(15-hydroxypentadecyl)-2,4,4-trimethyl-2-cyclohexen-1-one; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D003512:Cyclohexanones; D005233:Fatty Alcohols; D012701:Serotonin; D000255:Adenosine Triphosphate; D020866:omega-Conotoxin GVIA; D009543:Nifedipine; D002118:Calcium