Lactobacillus fermentum CRL 722 is able to deliver active alpha-galactosidase activity in the small intestine of rats.
alpha-galactooligosaccharides (alpha-GOS) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack alpha-galactosidase (alpha-Gal) in the small intestine which is necessary for their hydrolysis. Lactobacillus fermentum CRL 722 is a lactic acid bacterium (LAB) capable of degrading alpha-GOS due to its elevated alpha-Gal activity. When conventional rats were fed live L. fermentum CRL 722 or cell-free extracts of this strain, a short-lived alpha-Gal activity was detected in the upper gastrointestinal tract. The safety of this LAB was also assessed. L. fermentum CRL 722 could thus be used as a vehicle to safely confer alpha-Gal in the small intestine of monogastric animal.
LeBlanc
Jean Guy
JG
Centro de Referencia para Lactobacilos (CERELA-CONICET). Chacabuco 145, San Miguel de Tucumán, Tucumán, Argentina (T4000ILC). leblanc@cerela.org.ar
Piard
Jean-Christophe
JC
Sesma
Fernando
F
de Giori
Graciela Savoy
GS
D000818:Animals; D017077:Culture Media, Conditioned / Q000378:metabolism; D004063:Digestion; D004353:Drug Evaluation, Preclinical; D007421:Intestine, Small / Q000378:metabolism* / Q000382:microbiology; D007778:Lactobacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D000519:alpha-Galactosidase / Q000378:metabolism*
D017077:Culture Media, Conditioned; D000519:alpha-Galactosidase