Identification of a novel gene, HCR2, in aorta of hypercoagulable rats using suppression subtractive hybridization.
Screening and identification of novel genes involved in hypercoagulable state (HCS) is important in understanding the underlying mechanisms of development of atheroslerosis, which implicated in critical vascular diseases such as coronary heart disease and stroke. HCS was induced in rats with high carbohydrate diet. Subtractive hybridization experiments between aorta tissues from hypercoagulable rats and controls showed that a novel cDNA (GenBank Accession No. AY234417), designated as hypercoagulability related gene-2 (HCR2), was highly expressed in aorta tissue. The predicted protein encoded by HCR2 contains 78 amino acids, which has a theoretical isoelectric point (pI) of 8.59 and molecular weight (MW) of 8841.7 based on sequence analysis. Our data suggest that HCR2 may be involved in HCS and identification of the gene may contribute to our understanding of the mechanisms for the production of a hypercoagulable state of several critical clinical disorders.
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, PR China.
Yu-Hua
YH
Zhao
Huai
H
Bai
Bing-Wen
BW
Liu
Tao
T
Shen
Ding-Zhi
DZ
Fang
Yu
Y
Liu
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013489:Suppression, Genetic; D019851:Thrombophilia / Q000378:metabolism*; D014018:Tissue Distribution
D006023:Glycoproteins; C503567:LOC100364673