Identification of Vibrio parahaemolyticus pandemic group-specific DNA sequence by genomic subtraction.
A genomic subtraction between a pandemic Vibrio parahaemolyticus and a nonpandemic strain that seemed to be clonally related was performed. A subtractive DNA fragment was identified to be a part of a 16-kbp insertion sequence which was present in almost all pandemic strains but not in nonpandemic strains tested.
Okura
Masatoshi
M
Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501.
Osawa
Ro
R
Arakawa
Eiji
E
Terajima
Jun
J
Watanabe
Haruo
H
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007564:Japan / Q000453:epidemiology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012703:Serotyping; D013045:Species Specificity; D014735:Vibrio Infections / Q000453:epidemiology*; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial