Gingival crevicular fluid EMAP-II, MIP-1alpha and MIP-1beta levels of patients with periodontal disease.
BACKGROUND
Periodontal diseases may differ, which could be attributed to the factors that might modify the host response to microbial pathogens. The aim of this study was to examine gingival crevicular fluid (GCF) levels of EMAP-II, MIP-1alpha and MIP-1beta in patients with different periodontal diseases (EMAP-II, endothelial-monocyte activating polypeptide; MIP-1alpha, macrophage inflammatory protein-1alpha; MIP-1beta, macrophage inflammatory protein-1beta).


METHODS
Eighty-two subjects were included in this study. GCF samples were collected from 26 patients with generalized aggressive periodontitis (G-AgP), 26 patients with chronic periodontitis (CP), 15 with gingivitis and 15 periodontally healthy subjects. Clinical periodontal parameters were recorded. GCF EMAP-II, MIP-1alpha and MIP-1beta levels were quantified by enzyme immunoassay.


RESULTS
GCF EMAP-II levels of G-AgP group were higher than those of gingivitis and healthy groups (p<0.008). G-AgP group showed a trend for higher GCF EMAP-II levels compared with CP group (p>0.008). G-AgP, CP, gingivitis and healthy groups had comparable GCF MIP-1alpha and MIP-1beta levels.


CONCLUSIONS
Our results suggest that elevated GCF EMAP-II could contribute to the pathogenesis of G-AgP. Alternatively, EMAP-II reflects the extent of the inflammatory activity in the periodontal tissues. At this point, MIP-1alpha and MIP-1beta levels in GCF do not seem to play a discriminatory role in periodontitis. Our data document for the first time the essential role of EMAP-II in the pathogenesis of different periodontal diseases.
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. gemingil@yahoo.com
Gülnur
G
Emingil
Gül
G
Atilla
Aykut
A
Başkesen
Afig
A
Berdeli
D000208:Acute Disease; D000328:Adult; D016022:Case-Control Studies; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002908:Chronic Disease; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005891:Gingivitis / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D018836:Inflammation Mediators / Q000032:analysis / Q000378:metabolism*; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D010512:Periodontal Index; D010518:Periodontitis / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D018709:Statistics, Nonparametric
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D018836:Inflammation Mediators; D019402:Macrophage Inflammatory Proteins; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; C077219:small inducible cytokine subfamily E, member 1