[Butyrylcholinesterase: 3D structure, catalytic mechanisms].
Cholinesterases are the main targets of organophosphorus compounds. Although progresses in prophylaxis and treatment of nerve agent poisoning have been achieved in the past twenty years, pharmacological protection and emergency treatment remain imperfect. All classical pharmacological ways have been explored and, the current medical counter-measure arsenal is generally not expected to change much. However, the emergence of the catalytic bioscavenger concept has aroused new hope. In addition, resolution of the three-dimensional structure of human butyrylcholinesterase (BChE) answered some fundamental issues in medical defense research: 1) a better description of cholinesterase catalytic mechanisms (substrate hydrolysis, inhibition by organophosphorus); 2) aging mechanism of phosphylated cholinesterases caused by the dealkylation of branched organophosphorous moiety; 3) reasoned site-directed mutagenesis of human BChE with the aim of making operational mutants capable of detoxifying organophosphorus substrates.
Nachon
Fl
F
Centre de Recherche du Service de Santé des Armées, Département de toxicologie, Unité d'enzymologie, BP 87, F38702 La Tronche Cedex. fnachon@crssa.net
Nicolet
Y
Y
Masson
P
P
D002091:Butyrylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular
D002800:Cholinesterase Inhibitors; D002091:Butyrylcholinesterase