Kinetics of protein binding in solid-phase immunoassays: theory.
In a solid-phase immunoassay, binding between an antigen and its specific antibody takes place at the boundary of a liquid and a solid phase. One of the reactants (receptor) is immobilized on a surface. The other reactant (ligand) is initially free in solution. We present a theory describing the kinetics of immunochemical reaction in such a system. A single essential restriction of the theory is the assumption that the reaction conditions are uniform along the binding surface. In general, the reaction rate as a function of time can be obtained numerically as a solution of a nonlinear integral equation. For some special cases, analytical solutions are available. Various immunoassay geometries are considered, in particular, the case when the reaction is carried out on a microspot.
Klenin
Konstantin V
KV
Division of Biophysics of Macromolecules, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120, Heidelberg, Germany.
Kusnezow
Wlad
W
Langowski
Jörg
J
D000465:Algorithms; D000937:Antigen-Antibody Reactions; D007118:Immunoassay / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D008962:Models, Theoretical; D011485:Protein Binding
D008024:Ligands