A newly identified HLA-C allele: Cw*1210, and confirmation of HLA-Cw*080102.
HLA-Cw*1210 and -Cw*080102 were identified in Taiwan populations using reverse-line SSO typing method. Sequencing showed that HLA-Cw*1210 and -Cw*120201/02 were identical at exon 2 but differed at exon 3 with three nucleotide substitutions at codons 99 and 105 and an amino acid change from tyrosine to phenylalanine at codon 99. Also, allele HLA-Cw*080102 differed from HLA-Cw*080101 in exon 3 by a single synonymous nucleotide transition at codon 99. HLA-Cw*080102 was found only in the Puyuma indigenous tribe of Taiwan and had a frequency of 5%. Interestingly, nucleotide substitutions of HLA-Cw*1210 and -Cw*080102 were located in a noticeably heterogeneous region of the 5' end of exon 3, where similar substitution motifs are frequently found in other HLA-C alleles, therefore suggesting preferential gene conversion in this region.
Chu
C C
CC
Transfusion Medicine Laboratory, Medical Research Department, Mackay Memorial Hospital, Taipei, Taiwan.
Lee
H L
HL
Chen
Z X
ZX
Lin
M
M
D000483:Alleles; D001483:Base Sequence; D003062:Codon; D005091:Exons; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014443:Tyrosine / Q000737:chemistry
D003062:Codon; D015236:HLA-C Antigens; C503974:HLA-C*08:01:02 antigen; C503973:HLA-C*12:10 antigen; D014443:Tyrosine; D010649:Phenylalanine