Localisation of fucoxanthin chlorophyll a/c-binding polypeptides of the centric diatom Cyclotella cryptica by immuno-electron microscopy.
Antisera were raised against the C termini of three fucoxanthin chlorophyll a/c-binding polypeptides, Fcp2, Fcp4, and Fcp6, of the centric diatom Cyclotella cryptica. Immunogold electron microscopy of ultrathin-sectioned cells indicated that Fcp2 and Fcp4 are present in almost the same amounts, whereas approximately 8- to 10-fold less gold label was registered for Fcp6. Immunogold electron microscopy of freeze-fracture replicas of thylakoid membranes showed that the C termini of at least Fcp2 and Fcp4 were located in the thylakoid lumen, thus demonstrating a 3-dimensional structure similar to that already described for the chlorophyll a/b-binding light-harvesting polypeptides of higher plants.
Elektronenmikroskopisches Zentrum, Klinikum der Friedrich-Schiller-Universität Jena, Jena, Germany.
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Westermann
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Rhiel
D000818:Animals; D015153:Blotting, Western; D017377:Diatoms / Q000201:enzymology* / Q000648:ultrastructure; D005614:Freeze Fracturing; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology; D007150:Immunohistochemistry; D045342:Light-Harvesting Protein Complexes / Q000276:immunology / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000379:methods; D011817:Rabbits
D007106:Immune Sera; D045342:Light-Harvesting Protein Complexes; C088752:fucoxanthin-, chlorophyll a-c-containing protein