Reflectometric interference spectroscopy combined with MALDI-TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives.
This paper describes the combination of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with label free bio-interaction analysis based on reflectometric interference spectroscopy (RIfS). The potential of this concerted approach is demonstrated by measuring the binding properties of different vancomycin-type glycopeptide antibiotic mixtures. Although RIfS is sensitive and does not require use of a label, it cannot determine which components of a mixture have bound to the surface after incubation. Fortunately, each bound species has a unique mass that can, afterwards, be determined by mass spectrometry. Thus, the screening capability of RIfS is combined with the identification capability of mass spectrometry.
Institute for Physical and Theoretical Chemistry, University of Tübingen, 72074 Tuebingen, Germany.
Martin
M
Mehlmann
Alex M
AM
Garvin
Michael
M
Steinwand
Günter
G
Gauglitz
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques; D010316:Particle Size; D010455:Peptides / Q000378:metabolism; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D010455:Peptides; D014640:Vancomycin