Crystallization and preliminary crystallographic studies of the recombinant antitumour lectin from the edible mushroom Agrocybe aegerita.
The antitumour lectin from Agrocybe aegerita, named AAL, shows strong inhibition effects on human and mouse tumour cells via apoptosis induction activity. Recombinant AAL (rAAL) has been expressed and purified. Both rAAL and rAAL-lactose complex have been crystallized and their X-ray diffraction data were collected to resolutions of 1.9 A and 1.6 A, respectively. Both crystals belong to space group P2(1) with unit cell parameters a = 53.20 A, b = 66.01 A, c = 57.86 A, beta = 109.38 and a = 53.38 A, b = 66.29 A, c = 58.02 A, beta = 109.03, respectively.
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, People's Republic of China.
Na
N
Yang
Xin
X
Tong
Ye
Y
Xiang
Ying
Y
Zhang
Hui
H
Sun
Da-Cheng
DC
Wang
D000363:Agaricales / Q000737:chemistry / Q000235:genetics*; D000970:Antineoplastic Agents / Q000737:chemistry; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D007785:Lactose / Q000737:chemistry; D037102:Lectins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D000970:Antineoplastic Agents; D005656:Fungal Proteins; D037102:Lectins; D011994:Recombinant Proteins; D007785:Lactose