[Cloning and function analysis of L-lactate dehydrogenase gene from Lactobacillus sp. MD-1].
It was constructed that a genomic DNA library from Lactobacillus sp. MD-1 yielding D, L-lactic acid. The gene encoding L-lactate dehydrogenase (L-LDH) was cloned from the genomic library of strain MD-1 by complementation in E. coli FMJ144 which was lactate dehydrogenase and pyruvate-formate lyase double defective mutant. The nucleotide sequence of the ldhL gene predicted a protein of 316 amino acid starting with ATG. The putative molecular weight of the L-LDH amino acid sequence was 33.84kD. A putative typical promoter (-35 and -10 boxes) had been observed in the 5' noncoding region. An rho-independent transcriptional terminator has been observed in the 3' noncoding region. Three highly conserved regions (Gly13 approximately Asp50, Asp73 approximately Ileul00 and Asn123 approximately Arg154) with several conserved residues had been identified. Gly13 approximately Asp50 was NADH-binding site domain. Asp73 approximately Ileu100 and Asn123 approximately Arg154 were reported to be the active site domains. The ldhL and the L-LDH of Lactobacillus sp. MD-1 showed the low identity and similarity with other Lactobacilli, and the highest percentage were 61.9% and 68.9% respectively. All the above indicated this gene is a novel ldhL.
Life College, Nankai University, Tianjin 300071, China.
Jian
J
Li
Yun
Y
Tang
Feng-Lai
FL
Liang
Xin-Ping
XP
Zhang
Ru-Lin
RL
Liu
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data
D007770:L-Lactate Dehydrogenase