[Effect of ginsenoside Rg1 on neprilysin expression induced by LPS in SK-N-SH cell line].
OBJECTIVE
To investigate the effect of ginsenoside Rg1 on the neprilysin (NeP) expression induced by LPS in SK-N-SH cell line.


METHODS
MTT was used to measure the survival rate of SK-N-SH cultured with ginsenoside Rg1 at different concentrations (5, 10, 20 micromol/L) and LPS (50 mg/L). The expression of NEP was measured by RT-PCR.


RESULTS
The survival rate of SK-N-SH was obviously inhibited by LPS, the expression of NEP was decreased. On the other hand, the above alteration induced by LPS was reversed by ginsenoside Rg1.


CONCLUSIONS
This study demonstrates that LPS can cause cell damage and the decrease of NEP expression. Ginsenoside Rg1 can protect SK-N-SH cells against the injury induced by LPS, increase NEP expression.
Lü
Junhua
J
Jinan University, Guangzhou 510632.
Huang
Feng
F
Liu
Yueli
Y
Hu
Haiyan
H
Zhang
Ronghua
R
Cai
Yu
Y
Yang
Li
L
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D036145:Ginsenosides / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides; D015260:Neprilysin / Q000096:biosynthesis* / Q000235:genetics; D009447:Neuroblastoma / Q000201:enzymology* / Q000473:pathology; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008070:Lipopolysaccharides; D015260:Neprilysin