Ebselen: a thioredoxin reductase-dependent catalyst for alpha-tocopherol quinone reduction.
The thioredoxin system, composed of thioredoxin (Trx), thioredoxin reductase (TrxR), and NADPH, is a powerful protein disulfide reductase system with a broad substrate specificity. Recently the selenazol drug ebselen was shown to be a substrate for both mammalian TrxR and Trx. We examined if alpha-tocopherol quinone (TQ), a product of alpha-tocopherol oxidation, is reduced by ebselen in the presence of TrxR, since TQ was not a substrate for the enzyme itself. Ebselen reduction of TQ in the presence of TrxR was caused by ebselen selenol, generated from fast reduction of ebselen by the enzyme. TQ has no intrinsic antioxidant activity, while the product of reduction of TQ, alpha-tocopherolhydroquinone (TQH(2)), is a potent antioxidant. The thioredoxin system dependence of ebselen to catalyze reduction of other oxidized species, such as hydrogen peroxide, dehydroascorbate, and peroxynitrite, is discussed. The ability of ebselen to reduce TQ via the thioredoxin system is a novel mechanism to explain the effects of the drug as an antioxidant in vivo.
Fang
Jianguo
J
The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Zhong
Liangwei
L
Zhao
Rong
R
Holmgren
Arne
A
D000818:Animals; D001393:Azoles / Q000494:pharmacology*; D002384:Catalysis; D006801:Humans; D054833:Isoindoles; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013880:Thioredoxin-Disulfide Reductase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000378:metabolism
D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D014810:Vitamin E; C042986:ebselen; C002421:tocopherylquinone; D013880:Thioredoxin-Disulfide Reductase