Virulence attenuation and live vaccine potential of aroA, crp cdt cya, and plasmid-cured mutants of Salmonella enterica serovar Abortusovis in mice and sheep.
Three live vaccine candidates of Salmonella enterica subspecies I serotype Abortusovis (aroA, cya crp cdt, and plasmid-cured strains) have been developed, and their efficacies in inducing humoral antibodies and protecting against abortion after challenge with wild-type strain SS44 were evaluated in sheep. Following estrus synchronization, animals were immunized 3 weeks after fertilization and boosted once 3 weeks later. Following challenge with wild-type SS44, pregnancy failure of vaccinated ewes was reduced compared to that of nonimmunized controls. Attenuation of each vaccine was also assessed in challenge experiments with nonimmunized pregnant ewes and in BALB/c mice. All three vaccine candidates appear to be safe for use in sheep and provide a model for the development of live vaccine candidates against naturally occurring ovine salmonellosis.
Dipartimento di Scienze Biomediche, Center for Biotechnology Development and Biodiversity Research, Università di Sassari, Italy.
Sergio
S
Uzzau
Gavino
G
Marogna
Guido Sisinnio
GS
Leori
Roy
R
Curtiss
Giuseppe
G
Schianchi
Bruce A D
BA
Stocker
Salvatore
S
Rubino
D000034:Abortion, Veterinary / Q000517:prevention & control; D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010957:Plasmids; D011247:Pregnancy; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology*; D019779:Salmonella enterica / Q000276:immunology*; D012756:Sheep; D014436:Typhoid-Paratyphoid Vaccines / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence
D022562:Salmonella Vaccines; D014436:Typhoid-Paratyphoid Vaccines; D014613:Vaccines, Attenuated; C087350:aroA Salmonella vaccine