[Expression and genetic immunization of hantaan virus G2 recombinant adenovirus].
OBJECTIVE
To express hantaan virus(HTNV) envelope glycoprotein G(2) recombinant adenovirus(Adeno-G(2)) in vero E6 cells and explore its property of inducing immune response.


METHODS
Vero E6 cells were infected with the HTNV Adeno-G(2) (100 MOI). The expression of Adeno-G(2) in the infected Vero E6 cells was detected by IFA. BALB/c mice were immunized with HTNV Adeno-G(2), then the immune response to Adeno-G(2) was tested by ELISA, microcell-culture neutralizing experiment and lymphocyte proliferation test (MTT colorimetry).


RESULTS
IFA detection showed the expression of Adeno-G(2) in the infected Vero E6 cells. The titer of specific antibody was 1:40; The low-titer neutralization antibody was also detected. But the lymphocyte proliferation reaction was not notable.


CONCLUSIONS
The HTNV Adeno-G(2) can stimulate BALB/c mice to develop specific humoral immune response instead of specific cell-mediated immunity. This study provides the experimental basis for the development of gene engineering vaccine of HFRS.
Wu
Xing-an
XA
Department of Microbiology, Faculty of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Yu
Lan
L
Hu
Gang
G
Zhang
Fang-lin
FL
Bai
Wen-tao
WT
Shi
Meng-yuan
MY
Xu
Zhi-kai
ZK
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D018063:Hantaan virus / Q000235:genetics* / Q000276:immunology*; D007114:Immunization; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C090197:glycoprotein G2, Hantavirus