Role of extracellular superoxide dismutase in patients under maintenance hemodialysis.
BACKGROUND
It has been well documented that free radical injury is involved in the progression of chronic renal failure. Extracellular superoxide dismutase (EC-SOD), localized on the endothelial cell surface, plays an important role in reducing oxidative stress especially in the vessels by binding to the endothelial cell surface via the heparin-binding domain. Although EC-SOD Arg213Gly, which cannot bind on endothelial cells, has been considered a polymorphism, the effect of EC-SOD on hemodialysis patients has not been well examined.


METHODS
In 178 hemodialysis patients, the following examinations were performed. EC-SOD Arg213Gly was examined by polymerase chain reaction (PCR)-induced mutation restriction analysis (PCR-IMRA). As indexes of atherosclerosis, the annual progression in intima-media thickness (DeltaIMT), plaque score, pulse wave velocity (PWV) and plasma-oxidized low-density lipoprotein (OxLDL) values were examined.


RESULTS
PCR-IMRA revealed that 20 of 178 patients possessed the mutation (11.2%), and the incidence was about twice as high as that in a previously reported Japanese population. Although there were no statistical differences in plaque score and PWV with and without EC-SOD Arg213Gly, DeltaIMT and plasma OxLDL values in patients with EC-SOD Arg213Gly were significantly higher than those in patients without the mutation.


CONCLUSIONS
EC-SOD Arg213Gly is an accelerating factor for the progression of renal failure and atherosclerosis.
Department of Diagnostic Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Masaaki
M
Nakamura
Yukio
Y
Ando
Keiko
K
Sasada
Katsuki
K
Haraoka
Mitsuharu
M
Ueda
Hiroaki
H
Okabe
Yoshihiro
Y
Motomiya
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D050197:Atherosclerosis / Q000209:etiology*; D001794:Blood Pressure; D002339:Carotid Arteries / Q000000981:diagnostic imaging; D005260:Female; D005838:Genotype; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000235:genetics / Q000502:physiology*; D014463:Ultrasonography
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D013482:Superoxide Dismutase