Dietary fat and an exogenous emulsifier increase the gastrointestinal absorption of a major soybean allergen, Gly m Bd 30K, in mice.
The mechanisms by which food allergens are absorbed and sensitized via the gastrointestinal tract have not been well characterized. In this study, the gastrointestinal absorption of a major soybean allergen, Gly m Bd 30K, in young and older mice, and the effects of dietary fat and exogenous emulsifier were investigated. In Expt. 1, Gly m Bd 30K [0, 500 or 2000 mg/kg body weight (BW)] was administered orally to 24-d-old mice, and blood was sampled at various time points over a 120-min period. Plasma Gly m Bd 30K was measured by sandwich ELISA and immunoblotting. Its concentration peaked at 30 min and was dose dependent. Intact Gly m Bd 30K and its 20-kDa fragments were identified in plasma after absorption. In Expt. 2, 24-d-old mice administered soy milk containing 1 mg Gly m Bd 30K showed a steady increase in plasma Gly m Bd 30K from 60 to 120 min that was significantly higher than that in 10-wk-old mice. In Expt. 3, when corn oil (5 or 30%) was coadministered with Gly m Bd 30K (2000 mg/kg BW) to 24-d-old mice, the plasma concentration increased significantly and generally reached a plateau after 30 min. The absorption after the coadministration of 30% corn oil and 3% sucrose fatty acid ester was higher than after the administration of 30% corn oil alone. Intact Gly m Bd 30K and its fragments that were < 20 kDa survived digestion and were absorbed into the blood. We propose that absorption was enhanced by fat carrier-mediated transport.
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Thanakorn
T
Weangsripanaval
Tatsuya
T
Moriyama
Tadashi
T
Kageura
Tadashi
T
Ogawa
Teruo
T
Kawada
D000485:Allergens; D000818:Animals; D052179:Antigens, Plant; D004041:Dietary Fats / Q000494:pharmacology*; D045703:Emulsifying Agents / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000502:physiology*; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010940:Plant Proteins / Q000493:pharmacokinetics*; D041942:Soy Milk; D030262:Soybean Proteins; D013997:Time Factors; D014018:Tissue Distribution
D000485:Allergens; D052179:Antigens, Plant; D004041:Dietary Fats; D045703:Emulsifying Agents; C103236:Gly m BD 30K protein, Glycine max; D010940:Plant Proteins; D030262:Soybean Proteins