Identification of genetic markers for Mycobacterium pinnipedii through genome analysis.
Tuberculosis in seals is caused by Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex. In this study, we evaluated the extent of genetic variability among Mycobacterium bovis and M. pinnipedii by microarray-based comparative genomics. We identified two deletions that are exclusive to M. pinnipedii: PiD1 that removes the orthologues of the M. tuberculosis genes Rv3530c and Rv3531c, and PiD2 that encompasses genes Rv1977 and Rv1978. Interestingly, a deletion overlapping the previously described RD2 region was identified in some isolates of Mycobacterium microti and further characterised.
Bigi
Fabiana
F
Institute of Biotechnology, CICVyA-INTA, 1712 Castelar, Argentina. fbigi@cicv.inta.gov.ar
Garcia-Pelayo
M Carmen
MC
Nuñez-García
Javier
J
Peralta
Andrea
A
Caimi
Karina C
KC
Golby
Paul
P
Hinds
Jason
J
Cataldi
Angel
A
Gordon
Stephen V
SV
Romano
Maria I
MI
D046069:Fur Seals / Q000382:microbiology; D005819:Genetic Markers; D014644:Genetic Variation; D016680:Genome, Bacterial; D009161:Mycobacterium / Q000235:genetics* / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D013045:Species Specificity
D005819:Genetic Markers