Angiotensin I converting enzyme (ACE) inhibitory peptide derived from the sauce of fermented blue mussel, Mytilus edulis.
The angiotensin I converting enzyme (ACE) inhibitory activity of fermented blue mussel sauce (FBMS) was investigated. Blue mussels were fermented with 25% NaCl (w/w) at 20 degrees C for 6 months and the resultant mixture was passed through a 40-mesh sieve, desalted using an electrodialyzer and then lyophilized. The IC(50) value of FBMS for ACE activity was 1.01 mg/ml. An ACE inhibitory peptide was purified from FBMS using Sephadex G-75 gel chromatography, SP-Sephadex C-25 ion exchange chromatography and reversed-phase high-performance liquid chromatography on a C(18) column. The IC(50) value of purified ACE inhibitory peptide was 19.34 microg/ml, and 10 amino acid residues of the N-terminal sequence was EVMAGNLYPG. The purified peptide was evaluated for antihypertensive effect in spontaneously hypertensive rats (SHR) following oral administration. Blood pressure significantly decreased after peptide ingestion. This result suggested that FBMS may have beneficial effects on hypertension.
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Jae-Young
JY
Je
Pyo-Jam
PJ
Park
Hee-Guk
HG
Byun
Won-Kyo
WK
Jung
Se-Kwon
SK
Kim
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D001794:Blood Pressure / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D045424:Complex Mixtures / Q000737:chemistry*; D003911:Dextrans; D005285:Fermentation; D020128:Inhibitory Concentration 50; D007723:Korea; D009943:Organophosphorus Compounds; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR
C043517:2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; D000806:Angiotensin-Converting Enzyme Inhibitors; D045424:Complex Mixtures; D003911:Dextrans; D009943:Organophosphorus Compounds; D010455:Peptides; C025614:sephadex