Influence of progesterone, pregnenolone and 17beta-hydroxyprogesterone on the function of bovine luteal cells treated with luteinizing hormone, noradrenaline and prostaglandin E2.
The aim of the present study was to investigate the influence of progesterone (P4), its precursor (pregnenolone; P5) and metabolite (17beta-hydroksyprogesterone; 17betaOHP4) on secretory function of bovine luteal cells on days 6-10 of the estrous cycle and on intracellular Ca2+ mobilization. The luteal cells were pre-incubated for 24 h and after change of medium they were incubated for 30 min with P5 and 17betaOHP4 (10(-5) each). Next, the medium was supplemented with LH (100 ng/ml), noradrenaline (NA; 10(-5) M) and prostaglandin (PG)E2 (10(-6) M), the cells were incubated for further 4 h and the medium was collected for P4 determination. Another set of luteal cells (5x10(4)/well) was incubated with P4, P5 and 17betaOHP4 at the dose of 10(-5) M each for 30 min and intracellular Ca2+ mobilization was measured every 5 s three times before and for 60 s after cells stimulation with LH, NA and PGE2. Metabolite of P4 did not affect the stimulatory effect of LH, PGE2 and NA on P4 secretion to the medium. Whereas all used steroids reduced calcium release from small but not from large luteal cells. It is suggested that steroids could temporary impair effect of luteotropins on the luteal cells via non-genomic way.
Duras
M
M
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Prawocheńskiego 5, 10-718 Olsztyn-Kortowo, Poland.
Brzósko
E
E
Kotwica
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle / Q000097:blood*; D002478:Cells, Cultured / Q000187:drug effects; D015232:Dinoprostone / Q000008:administration & dosage / Q000494:pharmacology; D004971:Estrus / Q000502:physiology; D005260:Female; D042341:Gonadal Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D006908:Hydroxyprogesterones / Q000008:administration & dosage / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D011284:Pregnenolone / Q000008:administration & dosage / Q000494:pharmacology; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology
D042341:Gonadal Hormones; D006908:Hydroxyprogesterones; D011374:Progesterone; D011284:Pregnenolone; D007986:Luteinizing Hormone; D015232:Dinoprostone; D002118:Calcium; D009638:Norepinephrine