Identification of ginsenosides in roots of Panax ginseng by HPLC-APCI/MS.
A new HPLC-APCI/MS method for the identification of ginsenosides has been developed. The analyses were performed on a reversed-phase C18 column using a binary eluent (acetonitrile and water) under gradient conditions. Although APCI is a high-temperature evaporative process, HPLC-APCI/MS could effectively identify thermo-labile ginsenosides. The [M-H]- ions and the thermal degradation ions of ginsenosides could be clearly observed under negative and positive ion conditions, respectively, and these were used to identify the molecular masses, the aglycone structures and the sugar groups of ginsenosides. APCI/MS can provide more explicit information than ESI/MS for identifying and distinguishing ginsenosides. Using the HPLC-APCI/MS method, 35 ginsenosides were identified in Panax ginseng.
Ma
Xiao-Qiong
XQ
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, People's Republic of China.
Liang
Xin-Miao
XM
Xu
Qing
Q
Zhang
Xiao-Zhe
XZ
Xiao
Hong-Bin
HB
D002681:China; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
D036145:Ginsenosides