Treatment of YAC128 mice and their wild-type littermates with cystamine does not lead to its accumulation in plasma or brain: implications for the treatment of Huntington disease.
Cystamine is beneficial to Huntington disease (HD) transgenic mice. To elucidate the mechanism, cystamine metabolites were determined in brain and plasma of cystamine-treated mice. A major route for cystamine metabolism is thought to be: cystamine --> cysteamine --> hypotaurine --> taurine. Here we describe an HPLC system with coulometric detection that can rapidly measure underivatized cystamine, cysteamine and hypotaurine, as well as cysteine and glutathione in the same deproteinized tissue sample. A method is also described for the coulometric estimation of taurine as its isoindole-sulfonate derivative. Using this new methodology we showed that cystamine and cysteamine are undetectable (< or = 0.2 nmol/100 mg protein) in the brains of 3-month-old HD transgenic (YAC128) mice (or their wild-type littermates) treated daily for 2 weeks with cystamine (225 mg/kg) in their drinking water. No significant changes were observed in brain glutathione and taurine but significant increases were observed in brain cysteine. Cystamine and cysteamine were not detected in the plasma of YAC128 mice treated daily with cystamine between the ages of 4 and 12 or 7 and 12 months. These findings suggest that cystamine is not directly involved in mitigating HD but that increased brain cysteine or uncharacterized sulfur metabolites may be responsible.
Pinto
John T
JT
Burke Medical Research Institute, White Plains, New York, New York 10605, USA.
Van Raamsdonk
Jeremy M
JM
Leavitt
Blair R
BR
Hayden
Michael R
MR
Jeitner
Thomas M
TM
Thaler
Howard T
HT
Krasnikov
Boris F
BF
Cooper
Arthur J L
AJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003538:Cystamine / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology*; D003543:Cysteamine / Q000097:blood / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006816:Huntington Disease / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013654:Taurine / Q000031:analogs & derivatives / Q000378:metabolism; D013997:Time Factors
D013654:Taurine; C003949:hypotaurine; D003543:Cysteamine; D005978:Glutathione; D003538:Cystamine