Synthesis and cellular uptake of cell delivering PNA-peptide conjugates.
The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.
School of Chemistry, Edinburgh University, Joseph Black Buildings, Edinburgh, UK EH9 3JJ.
Juan J
JJ
Díaz-Mochón
Laurent
L
Bialy
Jon
J
Watson
Rosario M
RM
Sánchez-Martín
Mark
M
Bradley
D001120:Arginine / Q000737:chemistry; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008968:Molecular Conformation; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids; D010455:Peptides; D001120:Arginine