Glycan moiety modifications of feline alpha1-acid glycoprotein in retrovirus (FIV, FeLV) affected cats.
alpha1-Acid glycoprotein (AGP) is considered one of the major acute phase proteins in cats. In humans, AGP is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. In this paper we present the feline AGPs (fAGP) glycan moiety modifications in the course of two prevalent feline diseases, the FIV (feline immunodeficiency virus) dependent feline acquired viral immunodeficiency and the feline leukemia virus (FeLV) associated lymphoma. The glycan moiety of fAGP was investigated by means of the binding of its oligosaccharides residues with specific lectins. Four lectins were used: Sambucus nigra agglutinin I and Maackia amurensis agglutinin lectins were used to detect sialic acid residues, Aleuria aurantia lectin was used to detect L-fucose residues and Concanavalin A was used to evaluate the degree of branching. It was found that fAGP undergoes several post-translational modifications of its glycan pattern: in particular the degree of sialylation is increased in FeLV-positive cats diagnosed with lymphoma, while FeLV-positive that did not presented any specific clinical signs cats do not present any increase of expression of sialic acid on the surface. Furthermore, FIV induced a modification of the glycan moiety of fAGP, which however varied widely among individuals. In order to determine the number and the position of oligosaccharide chains, the cDNA sequence of fAGP was also determined. The translation of the mature fAGP coding sequence gave rise to a sequence of 183 residues, with five potential N-glycosylation sites, but also with seven potential phosphorylation sites.
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, University of Milan, Via Celoria 10, 20133 Milano, Italy.
Vanessa
V
Pocacqua
Elena
E
Provasi
Saverio
S
Paltrinieri
Elena
E
Gelain
Claudio
C
Comunian
Fabrizio
F
Ceciliani
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016181:Feline Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D016089:Immunodeficiency Virus, Feline; D037102:Lectins / Q000378:metabolism; D005256:Leukemia Virus, Feline; D016582:Leukemia, Feline / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009961:Orosomucoid / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D037102:Lectins; D009961:Orosomucoid; D011134:Polysaccharides