MIDAW: a web tool for statistical analysis of microarray data.
MIDAW (microarray data analysis web tool) is a web interface integrating a series of statistical algorithms that can be used for processing and interpretation of microarray data. MIDAW consists of two main sections: data normalization and data analysis. In the normalization phase the simultaneous processing of several experiments with background correction, global and local mean and variance normalization are carried out. The data analysis section allows graphical display of expression data for descriptive purposes, estimation of missing values, reduction of data dimension, discriminant analysis and identification of marker genes. The statistical results are organized in dynamic web pages and tables, where the transcript/gene probes contained in a specific microarray platform can be linked (according to user choice) to external databases (GenBank, Entrez Gene, UniGene). Tutorial files help the user throughout the statistical analysis to ensure that the forms are filled out correctly. MIDAW has been developed using Perl and PHP and it uses R/Bioconductor languages and routines. MIDAW is GPL licensed and freely accessible at http://muscle.cribi.unipd.it/midaw/. Perl and PHP source codes are available from the authors upon request.
Romualdi
Chiara
C
Dipartimento di Biologia, CRIBI Biotechnology Centre, Università degli Studi di Padova Via Ugo Bassi 58/B, I-35121 Padua, Italy.
Vitulo
Nicola
N
Del Favero
Micky
M
Lanfranchi
Gerolamo
G
D000465:Algorithms; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods*; D020407:Internet; D016011:Normal Distribution; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012984:Software; D014584:User-Computer Interface