In vitro determination of the release kinetics of peptides and free amino acids during the digestion of food proteins.
The kinetics of peptide release during in vitro digestion of 4 protein sources (casein, cod protein, soy protein, and gluten) were investigated. Samples were sequentially hydrolyzed with pepsin (30 min) and pancreatin (2, 4, or 6 h) in a dialysis cell with continuous removal of digestion products. Nondialyzed digests were fractionated by ion-exchange chromatography and ultrafiltration. Animal proteins were digested at a greater rate than plant proteins. Target amino acids of specific enzymes appeared more rapidly in the dialyzed fractions when compared to other amino acids. Throughout the hydrolysis, nondialyzed digests contained a higher proportion of peptide mixtures with basic-neutral properties. Except for gluten, peptide fractions with molecular weights that exceeded 10 kDa (basic-neutral, BN > 10) were rapidly hydrolyzed during the first 2 h of pancreatin digestion. The kinetics of release and the composition of peptide fractions were different when the protein sources were compared. The analysis of amino acids revealed that threonine and proline proportions were relatively high in BN > 10 and in peptide fractions with molecular weight between 10-1 kDa (BN 10-1), while tyrosine, phenylalanine, lysine, and arginine predominated in the low molecular weight (<1 kDa) fractions. More resistant peptides were generally rich in proline and glutamic acid. The role of in vitro digestion assays in dietary protein quality evaluation is discussed.
Savoie
Laurent
L
Université Laval, Department of the Science of Food and Nutrition, Quebec G1K7P4, Canada. laurent.savoie@aln.ulaval.ca
Agudelo
Rafael A
RA
Gauthier
Sylvie F
SF
Marin
Johanne
J
Pouliot
Yves
Y
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001120:Arginine / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D004044:Dietary Proteins; D004063:Digestion; D005504:Food Analysis; D018698:Glutamic Acid / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D015233:Models, Statistical; D010179:Pancreas / Q000378:metabolism; D010194:Pancreatin / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014443:Tyrosine / Q000737:chemistry; D014462:Ultrafiltration
D000596:Amino Acids; D004044:Dietary Proteins; D010455:Peptides; D011506:Proteins; D018698:Glutamic Acid; D014443:Tyrosine; D010649:Phenylalanine; D010194:Pancreatin; D001120:Arginine; D011392:Proline; D010434:Pepsin A; D008239:Lysine