Effects of selected xenoestrogens on liver peroxisomes, vitellogenin levels and spermatogenic cell proliferation in male zebrafish.
Environmental estrogenic compounds or xenoestrogens can mimic natural estrogens and cause a variety of adverse effects on aquatic wildlife. The purpose of the present work was to investigate if xenoestrogens are able to cause proliferation of liver peroxisomes using zebrafish (Danio rerio) as a model. Adult male zebrafish were exposed for 15 days to 17beta-estradiol (E2) and the xenoestrogens dibutylphthalate (DBP), methoxychlor (MXC), 4-tert-octylphenol (OP) and 17alpha-ethynylestradiol (EE2). All five tested compounds caused significant proliferation of liver peroxisomes (p < 0.05) as indicated by increased peroxisomal surface and numerical densities and elevated activities of the peroxisomal beta-oxidation enzyme acyl-CoA oxidase (AOX). In the case of DBP, MXC and E2, positive significant correlations between peroxisomal density parameters and AOX were found. The treatments did not produce gross alterations in testis histology, but spermatogenic cell proliferation was disturbed in E2 and EE2-treated groups and vitellogenin levels increased significantly in fish exposed to MXC, OP, EE2 and E2 with respect to controls. Furthermore, a significant correlation between vitellogenin levels and AOX activity was found for MXC, OP and EE2 treatments, suggesting that for the latter xenoestrogens early estrogenic effects are associated with liver peroxisome proliferation. No such association occurred with typical peroxisome proliferators such as DBP.
Biologia Zelularra eta Histologia Laborategia, Zoologia eta Biologia Zelularra Saila, Zientzia eta Teknologia Fakultatea, UPV/EHU, 644 PK, E-48080 Bilbo, Basque Country, Spain.
Maren
M
Ortiz-Zarragoitia
Miren P
MP
Cajaraville
D042962:Acyl-CoA Oxidase / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004958:Estradiol / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008731:Methoxychlor / Q000633:toxicity*; D020675:Peroxisomes / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013091:Spermatogenesis / Q000187:drug effects*; D014819:Vitellogenins / Q000032:analysis*; D015027:Zebrafish
D010636:Phenols; D018809:Proliferating Cell Nuclear Antigen; D014819:Vitellogenins; C474055:octylphenol; D003993:Dibutyl Phthalate; D004997:Ethinyl Estradiol; D004958:Estradiol; D042962:Acyl-CoA Oxidase; D008731:Methoxychlor