Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA.
RNA interference (RNAi) has been shown to inhibit viral replication in some animals and plants. Whether the RNAi is functional in shrimp remains to be demonstrated. In vitro transcribed dsRNAs of YHV helicase, polymerase, protease, gp116, and gp64 were transfected into shrimp primary cell culture and found to inhibit YHV replication. dsRNA targeted to nonstructural genes (protease, polymerase, and helicase) effectively inhibited YHV replication. Those targeted structural genes (gp116 and gp64) were the least effective. These findings are the first evidence that RNAi-mediated gene silencing is operative in shrimp cells. This could be a powerful tool for studying gene function and to develop effective control of viral infection in shrimp.
The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakornpathom 73170, Thailand. mbwtr@mahidol.ac.th
Witoon
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Tirasophon
Yaowaluck
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Roshorm
Sakol
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Panyim
D000818:Animals; D002478:Cells, Cultured; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D008214:Lymphocytes / Q000821:virology*; D033561:Penaeidae / Q000235:genetics* / Q000821:virology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics*; D046828:Roniviridae / Q000502:physiology*; D014162:Transfection / Q000379:methods; D014779:Virus Replication / Q000235:genetics*
D012330:RNA, Double-Stranded