Development and immunophenotyping of squamous cell carcinoma xenografts: tools for translational immunology.
OBJECTIVE
The objectives of this study were to delineate methods for the development of primary squamous cell carcinoma (SCCHN) xenografts and to define human leukocyte antigen (HLA), melanoma-associated antigen (MAGE)-A3, and human papilloma virus (HPV) 16 antigenic expression in resultant cellular products.


METHODS
Prospective experimental model.


METHODS
Freshly isolated SCCHN xenografts were established in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice using a variety of methods. Resultant tumors were analyzed for expression patterns of HLA-A, MAGE-A3, and HPV 16. Appropriate controls were included to ensure the presence of human RNA.


RESULTS
Three xenografts were successfully established and passaged in vivo. Characterization of the resultant products revealed that one was positive for HLA-A2 at both the DNA and protein levels. One of the tumor lines expressed MAGE-A3, whereas none expressed HPV 16.


CONCLUSIONS
Freshly isolated SCCHN can be used to generate primary xenografts. Characterization of select patterns of protein expression in established xenografts is a precursor to the development of a mouse model for SCCHN using tumor bearing animals reconstituted with autologous patient leukocytes.
Department of Otorhinolaryngology--Head and Neck Surgery, University of Maryland Medical Center, Baltimore, Maryland 21201, USA.
Wei
W
Lin
Xioayu
X
Zhang
Zhaorong
Z
Chen
Nancy
N
Borson
Steve
S
Voss
Schuyler
S
Sanderson
Linda
L
Murphy
Peter
P
Wettstein
Scott E
SE
Strome
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D045744:Cell Line, Tumor / Q000276:immunology / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics / Q000276:immunology; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D007150:Immunohistochemistry; D016130:Immunophenotyping / Q000379:methods*; D051379:Mice; D016688:Mice, Inbred NOD; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D009368:Neoplasm Transplantation / Q000276:immunology* / Q000473:pathology*; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D011446:Prospective Studies; D012313:RNA; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D012334:RNA, Neoplasm / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D015789:HLA-A2 Antigen; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012313:RNA