Increased circulating endothelial progenitor cells are associated with survival in acute lung injury.
BACKGROUND
Repair of damaged endothelium is important in recovery from acute lung injury. In animal models, bone marrow-derived endothelial progenitor cells differentiate into mature endothelium and assist in repairing damaged vasculature.


OBJECTIVE
The quantity of endothelial progenitor cells in patients with acute lung injury is unknown. We hypothesize that increased numbers of circulating endothelial progenitor cells will be associated with an improved outcome in acute lung injury and the acute respiratory distress syndrome.


METHODS
Peripheral blood mononuclear cells from the buffy coat of patients with early acute lung injury (n=45), intubated control subjects (n=10), and healthy volunteers (n=7) were isolated using Ficoll density gradient centrifugation, and plated on fibronectin-coated cellware. After 24 hours, nonadherent cells were removed and replated on fibronectin-coated cellware at a concentration of 1x10(6) cells/well. Colony-forming units were counted after 7 days' incubation.


RESULTS
Endothelial progenitor cell colony numbers were significantly higher in patients with acute lung injury compared with healthy control subjects (p<0.05), but did not differ between patients with acute lung injury and intubated control subjects. However, in the 45 patients with acute lung injury, improved survival correlated with a higher colony count (p<0.04). Patients with acute lung injury with a colony count of >or= 35 had a mortality of 30%, compared with 61% in those with colony counts <35 (p<0.03), results that persisted in a multivariable analysis correcting for age, sex, and severity of illness.


CONCLUSIONS
An increased number of circulating endothelial progenitor cells in acute lung injury is associated with improved survival.
Division of Pulmonary, Allergy, and Critical Care, Department of Medicine, Emory University School of Medicine, and the Atlanta Venterans' Affairs Medical Center, Atlanta, GA 30335, USA. eburnha@emory.edu
Ellen L
EL
Burnham
W Robert
WR
Taylor
Arshed A
AA
Quyyumi
Mauricio
M
Rojas
Kenneth L
KL
Brigham
Marc
M
Moss
D000328:Adult; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012128:Respiratory Distress Syndrome / Q000097:blood* / Q000401:mortality*; D013234:Stem Cells / Q000502:physiology*