Expression of retinoblastoma protein in human growth hormone-secreting pituitary adenomas.
The retinoblastoma gene (RB1) is a tumor-suppressor gene in chromosomal region 13q14.2. Its role in the pathogenesis of pituitary tumors has not been fully clarified. Some studies have shown that losses in this chromosomal region are related to aggressive tumor behavior, although the retinoblastoma protein (pRB) is still expressed. Conversely, lack of expression of pRB was observed in one fourth of GH-secreting pituitary adenomas (GH-tumors). In order to further study the expression of pRB in GH-tumors, we evaluated this protein in 49 tumors from patients with acromegaly (20 noninvasive, 25 invasive, and 4 with no information) and 8 normal pituitaries using immunohistochemistry (IHC). Nuclear staining for pRB ranged from 0 to 90% (median 40%) in the tumors and from 40 to 80% (median 58%) in normal pituitaries. In 10 tumors (20% of total) the adenomatous cells were negative (5 cases) or had very low labeling (5 cases) for pRB. Sixty three percent (31/49) of the tumors showed staining in 10-80% of the cells and in 16% (8/49) of the cases >80% of the adenomatous cells were positive for pRB. The expression of pRB was not different in invasive and noninvasive tumors. In conclusion, pRB is underexpressed in a subgroup of GH-tumors, and this may represent an early event in the pathogenesis of this tumor subtype.
Donangelo
Ines
I
Endocrine Unit, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro.
Marcos
Heliomar Pereira
HP
Araújo
Paula Bruna
PB
Marcondes
Jorge
J
Filho
Paulo Niemeyer
PN
Gadelha
Mônica
M
Chimelli
Leila
L
D000172:Acromegaly / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D000236:Adenoma / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D002452:Cell Count; D002648:Child; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000473:pathology; D010911:Pituitary Neoplasms / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D016160:Retinoblastoma Protein / Q000378:metabolism*
D016160:Retinoblastoma Protein; D019382:Human Growth Hormone