Structural and biological characterization of aminated-derivatized oat beta-glucan.
beta-Glucan extracted from oats was subjected to reductive amination, producing a cationic beta-glucan derivative of which physiological properties were characterized. The degree of substitution was obtained from elemental analysis, which was 0.48. In addition, the distribution of amino groups in the beta-glucan derivative was investigated by FT-IR analysis. In vitro bile acid binding capacity of the aminated beta-glucan was examined, showing significantly higher bile acid binding activity than native beta-glucan. Moreover, the beta-glucan derivative showed pronounced antimicrobial effects against Escherichia coli and Bacillus subtilis, and ACE (angiotensin-converting enzyme) inhibition activities which were dependent on its concentration. Furthermore, bronchoalveolar lavage (BAL) experiments demonstrated that the beta-glucan derivative stimulated the synthesis of nitric oxide. The improved functionalities of the derivative could be explained by its polycationic characteristics.
Department of Food and Nutrition, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Korea.
Min Su
MS
Shin
Suyong
S
Lee
Kwang Yeon
KY
Lee
Hyeon Gyu
HG
Lee
D000588:Amines / Q000737:chemistry*; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents; D018554:Avena / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D001647:Bile Acids and Salts / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002412:Cations; D004926:Escherichia coli / Q000187:drug effects; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000588:Amines; D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D002412:Cations; D005936:Glucans; D009569:Nitric Oxide