Identification of a CaRAV1 possessing an AP2/ERF and B3 DNA-binding domain from pepper leaves infected with Xanthomonas axonopodis pv. glycines 8ra by differential display.
We isolated a cDNA clone, CaRAV1, which exhibited significant similarity to those of Arabidopsis RAV proteins containing AP2/ERF and B3-like DNA-binding domains. CaRAV1 expression was rapidly and specifically induced in both host and non-host resistant responses against bacterial pathogens in the chili pepper plant. CaRAV1 also strongly increased following salicylic acid and ethephon treatments, whereas methyl-jasmonate only had mild effects. Furthermore, CaRAV1 transcript levels were also investigated in response to ABA and abiotic stress. No significant CaRAV1 expression was evident following ABA, mannitol, or cold treatments. These observations collectively provide initial evidence that the pepper RAV transcription factor homolog may function in plant defense responses.
Kim
Soo-Yong
SY
Plant Genomics Lab., Division of Genomics, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusung, Daejon 305-600, Republic of Korea.
Kim
Young-Cheol
YC
Lee
Jeong-Hee
JH
Oh
Sang-Keun
SK
Chung
Eunsook
E
Lee
Sanghyeob
S
Lee
Yong-Hwan
YH
Choi
Doil
D
Park
Jeong Mee
JM
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology; D029681:Arabidopsis Proteins / Q000235:genetics*; D002212:Capsicum; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005615:Freezing; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005998:Glycine / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics*; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014974:Xanthomonas / Q000502:physiology*
C499534:APETALA2 protein, Arabidopsis; D000085:Acetates; D000890:Anti-Infective Agents; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C117695:RAV1 protein, Arabidopsis; D008353:Mannitol; C072239:methyl jasmonate; D020156:Salicylic Acid; D005998:Glycine; C005073:ethephon