[Mechanism of apoptin-induced apoptosis of human lymphoma cells U937].
OBJECTIVE
To study the role of c-Jun N-terminal kinase (JNK) in the apoptosis induced by apoptin gene in human lymphoma cell U937.


METHODS
The U937 cells were transiently transfected by pcDNAA3 plasmids containing apoptin gene. Apoptosis of U937 cells was measured by flow-cytometry. Activation of JNK signal pathway was detected by Western blot.


RESULTS
Apoptin could induce apoptosis of U937 cells in vitro at 48 h after transfection. The level of phosphorylated JNK was increased at 24 h and reached the peak level at 48 h after transfection.


CONCLUSIONS
Apoptin gene can induce apoptosis of U937 cells in which JNK signal pathway plays an important role.
Lab. of Molecular Biology, Center for Diseases Control and Prevention, Shenyang Military Command, Shenyang 110034,China.
Song-bin
SB
Ben
Hong-li
HL
Zhao
Jing
J
Nie
Hong-yang
HY
Li
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008223:Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D020298:U937 Cells
D036022:Capsid Proteins; D012333:RNA, Messenger; C091717:VP3 protein, Chicken anemia virus; D048031:JNK Mitogen-Activated Protein Kinases