Neoflavonoids and related constituents from Nepalese propolis and their nitric oxide production inhibitory activity.
A methanolic extract of Nepalese propolis yielded 10 new open-chain neoflavonoids (1-10), a new chalcone (11), and eight previously reported compounds (12-19). Their structures were determined on the basis of spectroscopic data and chemical conversion. The isolated compounds other than 5, 8, and 16 showed dose-dependent inhibitory activities on nitric oxide production in lipopolysaccharide-activated macrophage-like J774.1 cells and were more active than a positive control, N(G)-monomethyl-L-arginine (IC(50), 27.1 microM). The most potent activities, of 6 and 7 (IC(50), 0.5 microM), were greater than another positive control, caffeic acid phenethyl ester (IC(50), 4.8 microM).
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
Suresh
S
Awale
Suraj Prakash
SP
Shrestha
Yasuhiro
Y
Tezuka
Jun-ya
JY
Ueda
Katsumichi
K
Matsushige
Shigetoshi
S
Kadota
D002109:Caffeic Acids / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D015394:Molecular Structure; D009390:Nepal; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011429:Propolis / Q000737:chemistry*; D019323:omega-N-Methylarginine / Q000494:pharmacology
D002109:Caffeic Acids; D005419:Flavonoids; D008070:Lipopolysaccharides; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D011429:Propolis; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol