Characterization of a reuterin-producing Lactobacillus coryniformis strain isolated from a goat's milk cheese.
Lactobacillus coryniformis CECT 5711, a strain isolated from a goat's milk cheese, displayed a broad-spectrum antimicrobial activity; as a consequence, its ability to produce the antagonistic compounds associated to lactic acid bacteria, including bacteriocins, hydrogen peroxide, lactic acid, acetic acid, and reuterin (3-hydroxypropionaldehyde, 3-HPA) was investigated. Production of bacteriocins or hydrogen peroxide by this strain could not be detected. However, in addition to lactic acid and acetic acid, it produced reuterin and cobalamin, a cofactor required for conversion of glycerol to 3-HPA through a glycerol dehydratase. The gene encoding a glycerol dehydratase subunit was detected by PCR and the corresponding amplicon was sequenced. This strain showed a high survival after exposition to conditions simulating those existing in the gastrointestinal tract as well as a notable ability to adhere to intestinal cells, which suggests that its reuterin-producing ability may be used for the host benefit. In addition, the strain showed a strong beta-galactosidase activity. Production of biogenic amines and degradation of mucin could not be detected.
Martín
R
R
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Olivares
M
M
Marín
M L
ML
Xaus
J
J
Fernández
L
L
Rodríguez
J M
JM
D000447:Aldehydes / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000096:biosynthesis; D002611:Cheese / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D006041:Goats; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism*; D008892:Milk / Q000382:microbiology*; D011407:Propane / Q000032:analysis* / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000447:Aldehydes; D001430:Bacteriocins; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D001616:beta-Galactosidase; D014805:Vitamin B 12; D011407:Propane