Inhibitory effects of sasanquasaponin on over-expression of ICAM-1 and on enhancement of capillary permeability induced by burns in rats.
The purpose of this study was to investigate the effects of sasanquasaponin (SQS) on ICAM-1 expression and capillary permeability induced by burns in rats. Male adult Sprague-Dawley (SD) rats were subjected to burns in the presence or absence of SQS, and then intravenously injected with Evans blue (60.0 mg/kg body weight). The levels of soluble ICAM-1 (sICAM-1) in sera were assayed using ELISA and the expression levels of transmembrane ICAM-1 (mICAM-1) in aorta were determined by Western blots and ICAM-1 mRNA levels were measured using semi-quantification RT-PCR. The capillary permeability was determined spectrophotometrically. The results showed that SQS markedly lowered the levels of sICAM-1 in sera, and considerably inhibited the over-expression as well as transcription of mICAM-1 in rat aorta. In addition, SQS dramatically inhibited the enhancement of dermal capillary permeability induced by burns in a dose-dependent manner. These results suggest that SQS, developed from Chinese traditional herbs, might be effective in decreasing inflammation induced by burns.
Huang
Qiren
Q
Department of Pharmacology, Jiangxi Medical College, Ba-Yi Road 603, Nanchang 330006, PR China. huangqiren67@hotmail.com
Shao
Lijian
L
He
Ming
M
Chen
Heping
H
Liu
Dan
D
Luo
Yongming
Y
Dai
Yucheng
Y
D000818:Animals; D001011:Aorta / Q000378:metabolism; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*
C497504:22-O-angeloylcamelliagenin C-3-O-(glucopyranosyl-1-2)(glucopyranosyl-1-2-O-arabinopyranosyl-1-3-)glucopyranosiduronic acid; D012503:Saponins; D018799:Intercellular Adhesion Molecule-1