The melanocortin-1 receptor carboxyl terminal pentapeptide is essential for MC1R function and expression on the cell surface.
The pigmentary actions of the melanocortins are mediated by the melanocortin-1 receptor (MC1R), a seven transmembrane domains receptor positively coupled to Gs and the cAMP cascade. In order to define the structure-function relationships of potentially relevant domains in MC1R, particularly its C-terminal cytosolic tail, we generated and analyzed several variants with C-terminal deletions, as well as point mutants in selected residues of the human MC1R. We show that the MC1R C-terminal pentapeptide is essential for proper receptor expression on the plasma membrane, and that residues Thr314, Cys315 and Trp317 are at least partially responsible for this effect.
Sánchez-Más
J
J
Department of Biochemistry and Molecular Biology, School of Medicine, University of Murcia, Apto 4021, 30100 Espinardo, Murcia, Spain.
Sánchez-Laorden
B L
BL
Guillo
L A
LA
Jiménez-Cervantes
C
C
García-Borrón
J C
JC
D000409:Alanine / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000502:physiology*; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D044102:Receptor, Melanocortin, Type 1 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D017384:Sequence Deletion; D014162:Transfection
D009842:Oligopeptides; D044102:Receptor, Melanocortin, Type 1; D003545:Cysteine; D000409:Alanine