Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography.
Four flavonoids, apigenin-7-O-neohesperidoside, luteolin-7-O-glucoside, apigenin-7-O-glucoside and kaempferol-7-O-glucoside have been isolated and purified for the first time from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography with a two-phase solvent system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v). Then, 5 mg apigenin-7-O-neohesperidoside, 4 mg luteolin-7-O-glucoside, 9 mg apigenin-7-O-glucoside and 2.5 mg kaempferol-7-O-glucoside could be obtained after injecting 40 mg sample and their purities were 94, 97, 97 and 96%, respectively. All these constituents were identified by mass spectrometry and nuclear magnetic resonance.
Wang
Xiao
X
Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
Cheng
Chuange
C
Sun
Qinglei
Q
Li
Fuwei
F
Liu
Jianhua
J
Zheng
Chengchao
C
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D029596:Paeonia / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids