Caspartin and calprismin, two proteins of the shell calcitic prisms of the Mediterranean fan mussel Pinna nobilis.
We used the combination of preparative electrophoresis and immunological detection to isolate two new proteins from the shell calcitic prisms of Pinna nobilis, the Mediterranean fan mussel. The amino acid composition of these proteins was determined. Both proteins are soluble, intracrystalline, and acidic. The 38-kDa protein is glycosylated; the 17-kDa one is not. Ala, Asx, Thr, and Pro represent the dominant residues of the 38-kDa protein, named calprismin. An N-terminal sequence was obtained from calprismin. This sequence, which comprises a pattern of 4 cysteine residues, is not related to any known protein. The second protein, named caspartin, exhibits an unusual amino acid composition, since Asx constitutes by far the main amino acid residue. Preliminary sequencing surprisingly suggests that the first 75 N-terminal residues are all Asp. Caspartin self-aggregates spontaneously into multimers. In vitro tests show that it inhibits the precipitation of calcium carbonate. Furthermore, it strongly interferes with the growth of calcite crystals. A polyclonal antiserum raised against caspartin was used to localize this protein in the shell by immunogold. The immunolocalization demonstrates that caspartin is distributed within the prisms and makes a continuous film at the interface between the prisms and the surrounding insoluble sheets. Our finding emphasizes the prominent role of aspartic acid-rich proteins for the building of calcitic prisms among molluscs.
UMR CNRS 5561 "Biogéosciences," Université de Bourgogne, 6 Boulevard Gabriel, Dijon F-21000, France. frederic.marin@u-bourgogne.fr
Frédéric
F
Marin
Reinout
R
Amons
Nathalie
N
Guichard
Martin
M
Stigter
Arnaud
A
Hecker
Gilles
G
Luquet
Pierre
P
Layrolle
Gérard
G
Alcaraz
Christophe
C
Riondet
Peter
P
Westbroek
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia / Q000737:chemistry* / Q000502:physiology*; D002119:Calcium Carbonate / Q000378:metabolism*; D003460:Crystallization; D004586:Electrophoresis; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D007118:Immunoassay; D008969:Molecular Sequence Data
D006023:Glycoproteins; D002119:Calcium Carbonate