[Sterilizing effect of atmospheric pressure plasma jet on microbes].
To study the sterilizing effect and mechanism of APPJ on microbes preliminarily, three representative bacteria, Staphylococcus aureus, Escherichia coli, Bacillus subtilis var. niger were treated by two kinds of plasma, DBD (dielectric barrier discharge) and APPJ. The survival curves of different microbes were compared and analyzed; Furthermore, the morphological change to cell walls and cell membranes were studied by scanning electric microscope. These results demonstrated that in the beginning phase the sterilizing effect of two kinds of plasmas on three microorganisms was stronger than in the later phase, and APPJ was more effective than DBD (D value of DBD on Bacillus subtilis var. niger was 70 s, whereas APPJ's was 4 s, much more efficient). Meanwhile, the gross morphological damage of E. coli cells under SEM after APPJ treatment was observed. These gave the obvious evidence that APPJ can destroy the microbes very efficiently, and more likely through the damage of cell walls and membranes of microbes treated by APPJ.
Wu
Xu-Qin
XQ
Institute of Hospital Infection Control & Research, PLA General Hospital, Beijing 100853, China. grglk369@sina.com
Wang
Shou-guo
SG
Han
Li
L
Zhao
Ling-li
LL
Chang
Xiao
X
Chen
Geng
G
Suo
Ji-jiang
JJ
Xing
Yu-bin
YB
Chen
Shi-ping
SP
D001412:Bacillus subtilis / Q000254:growth & development* / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development* / Q000648:ultrastructure; D005740:Gases; D008855:Microscopy, Electron, Scanning; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000648:ultrastructure; D013242:Sterilization
D005740:Gases