Effect of caspase inhibitors on the post-thaw motility, and integrity of acrosome and plasma membrane of cryopreserved equine spermatozoa.
The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addition of these caspase inhibitors to cryopreservation medium may not be beneficial in protecting the sperm from the stress of cryopreservation.
Peter
Augustine T
AT
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, Harrison Street, West Lafayette, IN 47907, USA. petera@purdue.edu
Colenbrander
Ben
B
Gadella
Barend M
BM
D000177:Acrosome / Q000378:metabolism; D020101:Acrosome Reaction; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D017304:Annexin A5 / Q000494:pharmacology; D061945:Caspase Inhibitors; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D005615:Freezing; D006736:Horses; D008297:Male; D019887:Peanut Agglutinin / Q000378:metabolism; D011419:Propidium / Q000494:pharmacology; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism*
D000590:Amino Acid Chloromethyl Ketones; D017304:Annexin A5; D061945:Caspase Inhibitors; D003451:Cryoprotective Agents; D004791:Enzyme Inhibitors; D019887:Peanut Agglutinin; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D011419:Propidium