[Screening of proteins interacting with Dishevelled2 in mouse 11.5dpc embryo library].
Dishevelled proteins are multifunctional and highly conserved. These proteins are also required for the specification of cell fate and polarity by secreted Wnt proteins. To investigate the molecular mechanism of Dishevelled in mediating Wnt signal transduction, a mouse 11.5dpc embryo library was screened by yeast-two-hybrid system to find mouse Dishevelled2 DEP domain and C-terminal interacting proteins. 15 possitive clones were identified from 4.1 x 10(6) transformants. The DNA sequences of the positive AD/library plasmids were determined. The BLAST results revealed that one of the positive clones contained N-terminus cDNA fragments (amino acids 6-122) of Gli3 protein. The interaction between Dv12 and Gli3 detected by yeast two-hybrid system suggests that Gli3 might play a role in some biological processes with Dishevelled.
Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology, Institute of Biomedicine, Tsinghua University, Beijing 100084, China.
Ser-Sue
SS
Ng
Yong-Gong
YG
Zhai
Liang
L
Han
Xin-Jun
XJ
Zhang
Yin-Yin
YY
Wang
Zhi-Jie
ZJ
Chang
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D000818:Animals; D000072261:Dishevelled Proteins; D015723:Gene Library; D051741:Kruppel-Like Transcription Factors / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000502:physiology*; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D010957:Plasmids; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D051153:Wnt Proteins / Q000502:physiology; D000074290:Zinc Finger Protein Gli3
D048868:Adaptor Proteins, Signal Transducing; D000072261:Dishevelled Proteins; C000606582:Dvl2 protein, mouse; C465571:Gli3 protein, mouse; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D051153:Wnt Proteins; D000074290:Zinc Finger Protein Gli3