Dimethyl sulfone in human cerebrospinal fluid and blood plasma confirmed by one-dimensional (1)H and two-dimensional (1)H-(13)C NMR.
(1)H-NMR spectroscopy at 500 MHz was used to confirm that a previously unidentified singlet resonance at 3.14 ppm in the spectra of cerebrospinal fluid and plasma samples corresponds to dimethyl sulfone (DMSO(2)). A triple resonance inverse cryogenic NMR probe, with pre-amplifier and the RF-coils cooled to low temperature, was used to obtain an (1)H-(13)C HSQC spectrum of CSF containing 8 microM (753 ng/ml) DMSO(2). The (1)H-(13)C correlation signal for DMSO(2) was assigned by comparison with the spectrum from an authentic reference sample. In plasma and CSF from healthy controls, the concentration of DMSO(2) ranged between 0 and 25 micromol/l. The concentration of DMSO(2) in plasma from three of four patients with severe methionine adenosyltransferase I/III (MAT I/III) deficiency was about twice the maximum observed for controls. Thus, DMSO(2) occurs as a regular metabolite at low micromolar concentrations in cerebrospinal fluid and plasma. It derives from dietary sources, from intestinal bacterial metabolism and from human endogenous methanethiol metabolism.
Radboud University Nijmegen Medical Centre, Laboratory of Pediatrics and Neurology, NL-6500 HB Nijmegen, The Netherlands.
Udo F H
UF
Engelke
Albert
A
Tangerman
Michèl A A P
MA
Willemsen
Detlef
D
Moskau
Sandra
S
Loss
S Harvey
SH
Mudd
Ron A
RA
Wevers
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid / Q000378:metabolism; D002247:Carbon Isotopes; D002648:Child; D002675:Child, Preschool; D004121:Dimethyl Sulfoxide; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008659:Metabolic Diseases / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D008875:Middle Aged; D009422:Nervous System Diseases / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D011522:Protons; D013450:Sulfones / Q000097:blood* / Q000134:cerebrospinal fluid*
D015415:Biomarkers; D002247:Carbon Isotopes; D011522:Protons; D013450:Sulfones; C025910:dimethyl sulfone; D004121:Dimethyl Sulfoxide