High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800.
The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.
Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Xiao-Zhou
XZ
Zhang
Zhong-Li
ZL
Cui
Qing
Q
Hong
Shun-Peng
SP
Li
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D008743:Methyl Parathion / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D008743:Methyl Parathion; D006867:Hydrolases; D008666:Metalloendopeptidases; C071298:bacillolysin