Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats.
OBJECTIVE
To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca(2+)]) and activity of sarcoplosnic Ca(2+)-ATPase (SRCa(2+)-ATPase) in Adriamycin (ADR)-treated rats.


METHODS
Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca(2+)] was detected by fluorescent spectrophotometry and the activity of SRCa(2+)-ATPase was detected by inorganic phosphate method.


RESULTS
FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa(2+)-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01).


CONCLUSIONS
FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa(2+)-ATPase activity in cardiomyocytes.
Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. weic236@sohu.com
Wei
W
Cai
Jun-zhu
JZ
Chen
Li-ming
LM
Ruan
Yi-na
YN
Wang
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage*; D004338:Drug Combinations; D004347:Drug Interactions; D004789:Enzyme Activation; D005635:Fructosediphosphates / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D019210:Troponin I / Q000097:blood*
D004338:Drug Combinations; D005635:Fructosediphosphates; D019210:Troponin I; D004317:Doxorubicin; D000252:Calcium-Transporting ATPases; C029063:fructose-1,6-diphosphate; D002118:Calcium