Enhancement of a pentacyclic tyrosine kinase inhibitor production in Cladosporium cf. cladosporioides by Cladosporol.
The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major pentacyclic metabolite 1, a cytokine production and tyrosine kinase inhibitor, was enhanced tenfold when cladosporol was supplied at the highest ratio (200 mg l(-1)) to shaken growing cultures of the fungus. The bioconversion of cladosporol to cladosporol D through reductive cleavage of the epoxide group was also observed. Interest in this kind of metabolites lies in their potential activity vs DNA topoisomerase I.
Assante
Gemma
G
Istituto di Patologia Vegetale, Facoltà di Agraria, Via Celoria 2, 20133 Milan, Italy. gemma.assante@unimi.it
Bava
Adriana
A
Nasini
Gianluca
G
D001711:Biotransformation; D002867:Chromones / Q000737:chemistry; D002963:Cladosporium / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism*; D015394:Molecular Structure; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D059004:Topoisomerase I Inhibitors
D002867:Chromones; D004791:Enzyme Inhibitors; D005449:Fluorenes; D006576:Heterocyclic Compounds, 4 or More Rings; D059004:Topoisomerase I Inhibitors; C081778:bulgarein; C027704:benzo(j)fluoranthene; D011505:Protein-Tyrosine Kinases