On the study of BSA-loaded calcium-deficient hydroxyapatite nano-carriers for controlled drug delivery.
Calcium-deficient hydroxyapatite (CDHA) nano-crystals incorporated with bovine serum albumin (BSA) to form BSA-loaded nano-carriers were synthesized via both in-situ and ex-situ processes. Amount of BSA uptake by the CDHA nano-crystals and subsequent release behaviors of the BSA-loaded nano-carriers were investigated. The amount of BSA uptake by CDHA decreases with increasing pH but a larger amount was observed in the ex-situ compared to in-situ process above pH=8.0. The release profile showed a bursting behavior for the nano-carrier prepared via the ex-situ process, which is probably due to the desorption of BSA molecules. In contrast, for the sample synthesized via the in-situ process at a higher pH level, a slower release profile without bursting behavior due to the dissolution of the BSA-incorporated CDHA crystal is seen from high solution TEM that indicates different extent of interaction between BSA and CDHA. On the other hand, for the nano-carriers prepared via the same process at lower pH level, a two-stage release profile was detected. An initial bursting release is due to the desorption of BSA from the CDHA surface, followed by a slow release as a result of the dissolution of the BSA-incorporated nano-crystals along its c-axis direction.
Department of Materials Science and Engineering, National Chiao Tung University 1001 Ta-hsueh Rd., Hsinchu, Taiwan, ROC.
Tse-Ying
TY
Liu
San-Yuan
SY
Chen
Dean-Mo
DM
Liu
Sz-Chian
SC
Liou
D000818:Animals; D002021:Buffers; D002118:Calcium / Q000737:chemistry*; D002417:Cattle; D003692:Delayed-Action Preparations / Q000737:chemistry*; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D002021:Buffers; D003692:Delayed-Action Preparations; D012710:Serum Albumin, Bovine; D017886:Durapatite; D002118:Calcium