Bone formation in calvarial defects of Sprague-Dawley rats by transplantation of calcium phosphate glass.
The purpose of this study was to investigate the bone-regenerative effect of calcium phosphate glass in vivo. We prepared amorphous calcium phosphate glass powder having a mean particle size of 400 microm in the system CaO-CaF2-P2O5-MgO-ZnO. Calvarial critical-sized defects (8 mm) were created in 60 male Sprague-Dawley rats. The animals were divided into an experimental group and control group of 30 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As a control, the defect was left empty. The rats were sacrificed 2, 4, or 8 weeks postsurgery, and the results evaluated using radiodensitometric and histological studies; they were also examined histomorphometrically. When the calcium phosphate glass powders with 400-microm particles were grafted, the defects were nearly completely filled with new-formed bone in a clean healing condition after 8 weeks. It was observed that the prepared calcium phosphate glass enhanced new bone formation in the calvarial defect of Sprague-Dawley rats and could be expected to have potential for use as a hard tissue regeneration material.
Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul 120-752, Korea.
Hyun-Ju
HJ
Moon
Kyoung-Nam
KN
Kim
Kwang-Mahn
KM
Kim
Seong-Ho
SH
Choi
Chong-Kwan
CK
Kim
Kee-Deog
KD
Kim
Racquel Z
RZ
LeGeros
Yong-Keun
YK
Lee
D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D002130:Calcium Phosphates; D017102:Fracture Healing / Q000502:physiology*; D005898:Glass; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000293:injuries*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C519480:amorphous calcium phosphate; C020243:calcium phosphate