The element(s) at the nontranscribed Xist locus of the active X chromosome controls chromosomal replication timing in the mouse.
In female mammalian cells, the inactive X chromosome is replicated late in S phase while the active X chromosome is replicated earlier. The replication times of the X chromosomes reflect a general trend in which late replication is associated with gene repression and earlier replication with transcriptional competence. The X-linked Xist gene is expressed exclusively from the inactive X chromosome where it is involved in the initiation and maintenance of X-inactivation. In contrast, no biological activity has been assigned to the Xist locus of the active X chromosome where the Xist gene is transcriptionally silenced. Here, we provide evidence that the element(s) at the nontranscribed Xist locus of the active X chromosome controls chromosomal replication timing in cis.
Department of Human Genetics, University of California, Los Angeles, 90095, USA.
Silvia
S
Diaz-Perez
Yan
Y
Ouyang
Vanessa
V
Perez
Roxanna
R
Cisneros
Moira
M
Regelson
York
Y
Marahrens
D000818:Animals; D001973:Bromodeoxyuridine; D017931:DNA Primers; D042522:DNA Replication Timing / Q000235:genetics*; D017353:Gene Deletion; D017404:In Situ Hybridization, Fluorescence; D051379:Mice / Q000235:genetics*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics*; D049951:X Chromosome Inactivation / Q000235:genetics*
D017931:DNA Primers; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C077885:XIST non-coding RNA; D001973:Bromodeoxyuridine