[Effects of culture conditions on coenzyme Q10 production by Rhizobium radiobacter by metabolic flux analysis].
Metabolic pathway network of CoQ10 synthesis by R. radiobacter WSH2601 were instructed. The metabolic flux and its changes were determined under the conditions of changing DO concentration and addition of 1% CSL in the medium. The results illustrated that the Ru5P flux (r7) increased by 26.6 when increasing the DO concentration, r7 increased by 17.2 when addition of 1% CSL. The ratio of EMP and HMP flux as well as TCA flux decreased at these two conditions. DPP flux had a little change at these two conditions. Therefore, the CoQ10 accumulation is greatly determined by two key enzymes activities of condensation reaction between p-hydroxybenzoate acid (PHB) and decaprenyl diphosphate (DPP). The nodes of G6P, pyruvate and PEP are principal nodes in primary metabolism of CoQ10 fermentation. The flexibility of principal nodes was evaluated that the G6P node is elastic, while pyruvate node is weakly flexibility, at the condition of changing culture conditions. The increase of DCW is associated with the improvement of HMP pathway flux.
Wu
Zu-fang
ZF
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China. wzjwpf@163.com
Du
Guo-cheng
GC
Chen
Jian
J
D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D003067:Coenzymes; D003470:Culture Media; D005285:Fermentation; D010100:Oxygen / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D000596:Amino Acids; D003067:Coenzymes; D003470:Culture Media; D014451:Ubiquinone; C024989:coenzyme Q10; D010100:Oxygen