Efficient differentiation of embryonic stem cells into neurons in glial cell-conditioned medium under attaching conditions.
Embryonic stem (ES) cells can differentiate into neurons in vitro, which provides hope for the treatment of some neurodegenerative diseases through cell transplantation. However, it remains a challenge to efficiently induce ES cells to differentiate into neurons. Here, we show that murine ES cells can efficiently differentiate into neurons when cultured in glial cell-conditioned medium (GCM) under attaching conditions without the formation of embryoid bodies. In comparison with murine embryonic fibroblast-conditioned medium, we found that GCM has a positive effect on limiting the generation of non-neuronal cells, such as astrocytes. In addition, compared with suspension conditions, attaching conditions delay the differentiation process of ES cells.
Tian
Hai-Bin
HB
Institute of Development Biology, School of life science, Shandong University, Jinan 250100, China.
Bai
Zeng-Liang
ZL
Wang
Hong
H
Chen
Jian-Quan
JQ
Cheng
Guo-Xiang
GX
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017077:Culture Media, Conditioned