Changes in activities of enzymes related to energy metabolism in canine lymphoma cells.
Alterations in the activities of enzymes related to energy metabolism in canine lymphoma cells were investigated. Cytosolic pyruvate kinase (PK) and mitochondrial malate dehydrogenase (MDH) activities in lymphoma cells were significantly higher than those in lymphocytes obtained from lymph nodes of healthy dogs, whereas cytosolic lactate dehydrogenase (LDH) activity was significantly lower in lymphoma cells. The cytosolic M/L ratio (MDH activity/LDH activity), which is considered to be a good indicator of energy metabolism related to glucose utilization in animal tissues, was significantly higher in lymphoma cells than in the normal lymphocytes.
Department of Clinical Pathology, Nippon Veterinary and Animal Science University, Musashino, Tokyo, Japan.
Tsukimi
T
Washizu
Daigo
D
Azakami
Makoto
M
Bonkobara
Makoto
M
Washizu
Toshiro
T
Arai
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D004734:Energy Metabolism / Q000502:physiology*; D006593:Hexokinase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology; D008223:Lymphoma / Q000201:enzymology / Q000662:veterinary*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D011770:Pyruvate Kinase / Q000378:metabolism; D013053:Spectrophotometry / Q000662:veterinary
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D006593:Hexokinase; D011770:Pyruvate Kinase