Antifungal coating by biofunctionalized polyelectrolyte multilayered films.
The surface of medical devices is a common site of bacterial and fungal adhesion, first step to the constitution of a resistant biofilm leading frequently to chronic infections. In order to prevent such complications, several physical and chemical modifications of the device surface have been proposed. Here, we experiment a new type of topical antifungal coating using the layer-by-layer technique. The nanometric multilayer film obtained by this technique is functionalized by the insertion of a chromogranin A-derived antifungal peptide (CGA 47-66, chromofungin). We show that the embedded peptide keeps its antifungal activity by interacting with the fungal membrane and penetrating into the cell. In vitro studies demonstrate that such an antifungal coating is able to inhibit the growth of yeast Candida albicans by 65% and completely stop the proliferation of filamentous fungus Neurospora crassa. The cytotoxicity of such a coating was also assessed by growing human gingival fibroblasts at its surface. Finally, the antifungal coating of poly(methylmethacrylate), a widely used material for biomedical devices, is successfully tested in an in vivo oral candidiasis rat model. Taken together, these results assessed the functionalized multilayer films containing a new potent antifungal non-toxic peptide, as a novel and promising technique for local antifungal protection.
Institut National de la Santé et de la Recherche Médicale, Unité 595, 11, rue Humann, 67085 Strasbourg Cedex, France.
Olivier
O
Etienne
Claire
C
Gasnier
Corinne
C
Taddei
Jean-Claude
JC
Voegel
Dominique
D
Aunis
Pierre
P
Schaaf
Marie-Helène
MH
Metz-Boutigue
Anne-Laure
AL
Bolcato-Bellemin
Christophe
C
Egles
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002176:Candida albicans / Q000378:metabolism; D053379:Chromogranin A; D002864:Chromogranins / Q000737:chemistry; D025141:Drug Resistance, Fungal; D004573:Electrolytes / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D051381:Rats; D017386:Sequence Homology, Amino Acid
D000935:Antifungal Agents; D001672:Biocompatible Materials; D053379:Chromogranin A; D002864:Chromogranins; D004573:Electrolytes; D010446:Peptide Fragments; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D019904:Polymethyl Methacrylate; C437333:chromofungin