Fatty acid desaturases from the microalga Thalassiosira pseudonana.
Analysis of a draft nuclear genome sequence of the diatom Thalassiosira pseudonana revealed the presence of 11 open reading frames showing significant similarity to functionally characterized fatty acid front-end desaturases. The corresponding genes occupy discrete chromosomal locations as determined by comparison with the recently published genome sequence. Phylogenetic analysis showed that two of the T. pseudonana desaturase (Tpdes) sequences grouped with proteobacterial desaturases that lack a fused cytochrome b5 domain. Among the nine remaining gene sequences, temporal expression analysis revealed that seven were expressed in T. pseudonana cells. One of these, TpdesN, was previously characterized as encoding a Delta11-desaturase active on palmitic acid. From the six remaining putative desaturase genes, we report here that three, TpdesI, TpdesO and TpdesK, respectively encode Delta6-, Delta5- and Delta4-desaturases involved in production of the health beneficial polyunsaturated fatty acid DHA (docosahexaenoic acid). Furthermore, we show that one of the remaining genes, TpdesB, encodes a Delta8-sphingolipid desaturase with strong preference for dihydroxylated substrates.
Tonon
Thierry
T
CNAP, Department of Biology, University of York, Heslington, York, UK.
Sayanova
Olga
O
Michaelson
Louise V
LV
Qing
Renwei
R
Harvey
David
D
Larson
Tony R
TR
Li
Yi
Y
Napier
Johnathan A
JA
Graham
Ian A
IA
D002849:Chromatography, Gas; D015786:Cytochromes b5 / Q000737:chemistry; D017377:Diatoms / Q000201:enzymology*; D004281:Docosahexaenoic Acids / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013107:Sphingolipids / Q000378:metabolism
D012333:RNA, Messenger; D018749:RNA, Plant; D013107:Sphingolipids; D004281:Docosahexaenoic Acids; D015786:Cytochromes b5; D044943:Fatty Acid Desaturases