Meningiomas expressing and responding to cholecystokinin (CCK).
The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism.
School of Health and Life Sciences, Biomedical Chemistry Research Group, Aston University, B4 7ET, Birmingham, UK.
Eftychia
E
Oikonomou
Ana L
AL
Machado
Michael
M
Buchfelder
Eric F
EF
Adams
D000328:Adult; D001483:Base Sequence; D001570:Benzodiazepinones / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008579:Meningioma / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010671:Phenylurea Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D001570:Benzodiazepinones; D010671:Phenylurea Compounds; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; C058121:L 365260; D002766:Cholecystokinin