Spectrofluorometric determination of trace amounts of coenzyme II using norfioxacin-terbium complex as a fluorescent probe.
When terbium ion (Tb3+)-norfloxacin (NFLX) complex is issued a fluorescent probe, in a buffer solution of pH = 7.6, NADP can remarkably enhance the fluorescence intensity of the Tb3+ -NFLX complex at lambda = 545 nm. The enhanced fluorescence intensity of Tb3+ is in proportion to the concentration of NADP. The dynamic range for the determination of NADP is 1.11 x 10(-7) - 6.16 x 10(-5) mol l(-1), with a detection limit of 4.31 x 10(-8) mol l(-1). This method is simple, practical and relatively free of interference from coexisting substances, so it can be successfully applied to determination of NADP in synthetic water samples.
Wang
Yusheng
Y
Department of Chemistry, Shandong Normal University, Jinan, 250014, China.
Liu
Jinkai
J
Jiang
Chongqiu
C
D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009249:NADP / Q000032:analysis* / Q000737:chemistry; D009643:Norfloxacin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*; D014867:Water / Q000032:analysis
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D014867:Water; D013725:Terbium; D009249:NADP; D009643:Norfloxacin