Characterization of cDNA and the genomic sequence encoding canine neural-cell adhesion molecule, CD56/N-CAM.
The neural-cell adhesion molecule, CD56/N-CAM is a member of the immunoglobulin superfamily expressed by various tissues and cells, including natural killer (NK) cells. Despite the importance of CD56 as a marker for identifying NK cells in circulating blood, canine CD56 has not been identified. In the present study, we identified the canine counterparts of the 140-kDa (CD56-140) and 120-kDa (CD56-120) isoforms of human DC56. Both of amino acid sequences encoded by the canine CD56-140 and -120 cDNA showed high homology with those of human (both 96% homology), having well-conserved domains (five immunoglobulin, two fibronectin type III, and transmembrane and intracellular or glycosylphosphatidylinositol-linked domain) among various species (human, mouse, and feline). We revealed that the transcripts of canine CD56-140 and -120 arise from alternative mRNA splicing from a single gene located on canine chromosome 5. Moreover, the mRNA encoding canine CD56-140 was expressed at high levels constitutively by nervous system and endocrine tissues as has shown in other animals.
Department of Veterinary Clinical Pathology, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan. bonkobara@nvau.ac.jp
M
M
Bonkobara
T
T
Sato
N
N
Takahashi
Y
Y
Kasahara
H
H
Yagihara
K
K
Tamura
M
M
Isotani
D
D
Azakami
K
K
Ono
T
T
Washizu
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019002:CD56 Antigen / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics* / Q000276:immunology*; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
D019002:CD56 Antigen; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger