[Changes of endogenous ouabain in rat serum and certain tissues after exposure to simulated weightlessness].
OBJECTIVE
To investigate the changes of endogenous ouabain (EO) in rat serum and some tissues after exposure to simulated weightlessness and to investigate its possible pathophysiology.


METHODS
Male Wistar rats were randomly divided into control group (Con) and 1 week tail-suspension group (TS). Enzyme-linked immunosorbent assay (ELISA) was used to detect the content of EO in serum, hypothalamus, pituitary, adrenal gland, kidney, heart and liver.


RESULTS
Compared with Con, EO increased significantly in serum, hypothalamus, adrenal gland and kidney after tail suspension (P<0.05).


CONCLUSIONS
Simulated weightlessness induced changes of EO in serum and some tissues, which may have some effects on the regulation of hydro-electrolyte metabolism and cardiovascular functions.
Liu
Zhao-xia
ZX
Department of Medicine, Xi'an Jiaotong University, Xi'an Shannxi, China. liuzx918@sohu.com
Gao
Guang-dao
GD
Hu
Yong-jie
YJ
Wang
De-sheng
DS
WU
Da-wei
DW
Yuan
Ming
M
Li
Zhi-li
ZL
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D019417:Hindlimb Suspension; D007031:Hypothalamus / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010042:Ouabain / Q000097:blood / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation
D010042:Ouabain