Characterization and transcription of the genes involved in butyrate production in Butyrivibrio fibrisolvens type I and II strains.
The genes in Butyrivibrio fibrisolvens that encode the enzymes involved in butyrate production were sequenced. In a type I strain (ATCC 19171(T)), the genes coding for the enzymes that catalyze the conversion from acetyl-CoA to butyryl-CoA, thl (thiolase), crt (crotonase), hbd (beta-hydroxybutyryl-CoA dehydrogenase), bcd (butyryl-CoA dehydrogenase), etfB (electron transfer flavoprotein [ETF]-beta), and etfA (ETF-alpha), were found to be clustered and arranged in this order. A type II strain (ATCC 51255) had the same clustered genes with the same arrangement, except that crt was not present in the clustered genes. The deduced amino acid sequences of these enzymes did not greatly differ between the two strains, and even between B. fibrisolvens and clostridia. Amino acid identity appeared to be higher within the same type than between types I and II. The clustered genes were shown to be cotranscribed, and constitutively transcribed without being affected significantly by culture conditions.
Asanuma
Narito
N
Department of Life Science, College of Agriculture, Meiji University, Higashimita, Tama-ku, Kawasaki 214-8571, Japan. asanuma@isc.meiji.ac.jp
Ishiwata
Masaya
M
Yoshii
Takahiro
T
Kikuchi
Masahiro
M
Nishina
Youichi
Y
Hino
Tsuneo
T
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics*; D000085:Acetates / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000235:genetics* / Q000378:metabolism; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D000214:Acyl Coenzyme A / Q000096:biosynthesis / Q000235:genetics*; D002087:Butyrates / Q000378:metabolism*; D045856:Butyrivibrio / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D000085:Acetates; D000214:Acyl Coenzyme A; D002087:Butyrates; D000105:Acetyl Coenzyme A; D015094:3-Hydroxyacyl CoA Dehydrogenases; D000101:Acetyl-CoA C-Acetyltransferase