Paraoxonase (PON1) 55 and 192 polymorphism and its effects to oxidant-antioxidant system in turkish patients with type 2 diabetes mellitus.
Paraoxonase (PON1) is a serum enzyme with an antioxidant function, protecting the low density lipoproteins (LDL) from oxidative modifications. Because diabetic patients are at greater risk of oxidative stress, we investigated the effect of PON1 55 methione (M)/leucine (L) and PON1 192 glutamine (A)/arginine (B) polymorphisms on oxidant-antioxidant system in 213 individuals with type 2 diabetes mellitus and 116 non-diabetic control subjects from Turkish population were included in the study. Polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and agarose gel electrophoresis techniques were used to determine the PON1 genotypes. Thiobarbituric acid reactive substances (TBARS), conjugated dienes levels in the serum and glutathione (GSH) levels in whole blood were measured spectrophotometrically. In both groups PON1 192 AA and PON1 55 MM genotypes had higher TBARS, conjugated dienes levels and lower GSH levels, whereas PON1 192 BB and PON1 55 LL genotypes had lower TBARS, conjugated diene levels and higher GSH level than other genotypes. We thus conclude that PON1 192 BB and PON1 55 LL alleles have protective effect against oxidative stress.
University of Istanbul, Institute for Experimental Medical Research, Department of Molecular Medicine, Istanbul, Turkey.
B
B
Agachan
H
H
Yilmaz
H A
HA
Ergen
Z E
ZE
Karaali
T
T
Isbir
D043303:Aryldialkylphosphatase / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D005820:Genetic Testing / Q000379:methods*; D005978:Glutathione / Q000097:blood*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D017382:Reactive Oxygen Species / Q000097:blood; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*; D014421:Turkey / Q000453:epidemiology
D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D043303:Aryldialkylphosphatase; D005978:Glutathione