[Establishment and preservation of immortal lymphoblastoid cell lines of the 10 ethnic groups in China].
The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhibit the activity of T cells. In the present study,549 immortal cell lines of different ethnic groups of Hazak, Manchu, Korea, Hozhe, Mongolia, Sibe, Hui, Puyi, Han in Fujian and Han in Sichuan were established. Through our research,we found it is harmful for B lymphocytes to transform if excessful leucocytes are inoculated.And it is crisis that cyclosporine A shoud be added the last. Our work is an important part of the research of human genome diversity for the exploration of the origin and evolution of different ethnic groups,and it also provides enough research materials for further studies.Moreover,we have sent 50 cell lines of Hozhe, Mongolia, Sibe, Daur, Oroqen to CEPH. Thus it is possible for us to utilize the genetic resources of CEPH freely.
Department of Medical Genetics, Harbin Medical University, Harbin 150086, China.
Xiao-yi
XY
Huang
An
A
Liu
Yang
Y
Yu
Lin-lin
LL
Ma
Rong-qian
RQ
Shi
Fu-qu
FQ
Lü
Yan
Y
Jiang
Wen-jing
WJ
Sun
Ya-li
YL
Xue
Song-bin
SB
Fu
Pu
P
Li