Synthesis of hydroquinone-alpha-glucoside by alpha-glucosidase from baker's yeast.
Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation reaction were at 30 degrees C for 20 h with 50 mM: hydroquinone and 1.5 M: maltose in 100 mM: sodium citrate/phosphate buffer at pH 5.5. The glucoside was obtained at 0.6 mg/ml with a 4.6% molar yield with respect to hydroquinone.
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia and Montenegro. rprodano@chem.bg.ac.yu
Radivoje M
RM
Prodanović
Nenad B
NB
Milosavić
Dusan
D
Sladić
Tanja Cirković
TC
Velicković
Zoran
Z
Vujcić
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005960:Glucosides / Q000032:analysis / Q000096:biosynthesis*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008320:Maltose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*
D005960:Glucosides; D006873:Hydroquinones; D029701:Saccharomyces cerevisiae Proteins; C096512:hydroquinoneglucoside; D008320:Maltose; D000520:alpha-Glucosidases; C031927:hydroquinone