Role of microfilaments and microtubules in the invasion of EPC cells by Aeromonas hydrophila.
The influence of the cytoskeleton on the invasion of Aeromonas hydrophila strain AhJ-1, isolated from diseased fish, in the monolayer cell of epithelioma papillosum cells of carp (EPC) was evaluated by the recovery of gentamicin-resistant bacteria from Triton X-100 cell lysates. The depolymerization of microfilaments (MF) by cytochalasin B and D inhibited the uptake of A. hydrophila in a dose-dependent manner and that of microtubules (MT) by colchicines and nocodazole did not affect the invasion of A. hydrophila in EPC cells significantly. The invasion frequency decreased approximately 62% with the addition of 0.1 microg/ml cytochalasin D and nearly 86% by the addition of 5.0 microg/ml. Invasion decreased approximately 49% and 83% by addition of cytochalasin B in a concentration of 2.5 microg/ml and 10.0 microg/ml. Colchicine and nocodazole, inhibitors of MT formation appears to have little effect on the invasion of EPC cells by strain Ah J-1. Thus MF formation, but not MT formation seems to play an important role in the internalization of A. hydrophila J-1.
Chu
W H
WH
Department of Bioengineering, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. chuweihua@yahoo.com.cn
Lu
C P
CP
D008841:Actin Cytoskeleton / Q000502:physiology; D016980:Aeromonas hydrophila / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D002347:Carps; D002478:Cells, Cultured / Q000382:microbiology; D003572:Cytochalasins / Q000008:administration & dosage / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000382:microbiology; D005393:Fish Diseases / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D007422:Intestines / Q000382:microbiology; D008870:Microtubules / Q000502:physiology
D003572:Cytochalasins