Health and reproductive profiles of malaria antigen-producing transgenic goats derived by somatic cell nuclear transfer.
Nuclear transfer (NT) using transfected primary cells is an efficient approach for the generation of transgenic goats. However, reprogramming abnormalities associated with this process might result in compromised animals. We examined the health, reproductive performance, and milk production of four transgenic does derived from somatic cell NT. Goats were derived from two fetal cell lines, each transfected with a transgene expressing a different version of the MSP-1(42) malaria antigen, either glycosylated or non-glycosylated. Two female kids were produced per cell line. Health and growth of these NT animals were monitored and compared with four age-matched control does. There were no differences in birth and weaning weights between NT and control animals. The NT does were bred and produced a total of nine kids. The control does delivered five kids. The NT does expressing the glycosylated antigen lactated only briefly, probably as a result of over-expression of the MSP-1(42) protein. However, NT does expressing the non-glycosylated antigen had normal milk yields and produced the recombinant protein. These data demonstrated that the production of healthy transgenic founder goats by somatic cell NT is readily achievable and that these animals can be used successfully for the production of a candidate Malaria vaccine.
Behboodi
E
E
GTC Biotherapeutics, Inc., Framingham, Massachusetts 01701, USA.
Ayres
S L
SL
Memili
E
E
O'Coin
M
M
Chen
L H
LH
Reggio
B C
BC
Landry
A M
AM
Gavin
W G
WG
Meade
H M
HM
Godke
R A
RA
Echelard
Y
Y
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000502:physiology; D001522:Behavior, Animal; D019976:Cloning, Organism; D004971:Estrus; D006031:Glycosylation; D006041:Goats / Q000235:genetics / Q000502:physiology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008892:Milk; D053652:Nuclear Transfer Techniques; D010961:Plasmodium / Q000276:immunology; D012098:Reproduction
D020066:Merozoite Surface Protein 1