[Long distance vectorette PCR for detecting translocation of IgH gene in switch regions of patients with multiple myeloma].
Chromosomal translocations involving immunoglobulin heavy chain (IgH) locus at 14q32 are commonly seen in multiple myeloma (MM), which may involve in pathogenesis and associate with prognosis. To rapidly detect the occurrence of translocation involving IgH locus and the fellow chromosomes and isolate the break points, According to the break points occured in switch regions, patents and references, a long distance vectorette PCR method was developed. The results showed that using this method, a 3.5 kb PCR product from MM cell line U266 was gained, each ends aligned with 11q and IgHalpha 1 switch region, respectively. It is concluded that LDV-PCR is effective detection technique. Detecting China MM patients by using this method can interpret some specific clinic statues and pathogenesis mechanisms, and help to find new therapy targets and to assess the patient prognosis.
Guo
Cha
C
Department of Hematology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China.
Huo
Jian
J
Jiang
Hua
H
D001483:Base Sequence; D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007134:Immunoglobulin Switch Region / Q000235:genetics*; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014178:Translocation, Genetic
D007143:Immunoglobulin Heavy Chains