PhoR1-PhoP1, a third two-component system of the family PhoRP from Myxococcus xanthus: role in development.
The pair PhoR1-PhoP1 is the third two-component system of the family PhoRP reported in M. xanthus. PhoR1 is a histidine kinase anchored to the membrane through a transmembrane domain located in the amino-terminal portion of the protein. As a result, 93% of the protein is located in the cytoplasm. This topology is unusual in the PhoR-type histidine kinases. PhoP1 is a response regulator with a helix-loop-helix motif typical of the DNA-binding proteins. Although the operon phoPR1 is expressed during vegetative growth, it peaks during development. The expression levels of this operon are higher in phosphate-containing media than in those in which the nutrient is absent. A deletion mutant in this system exhibits a delay in aggregation and the formation of fruiting bodies larger than those of the wild-type strain. The expression of the operon is autoregulated. This system is also partially responsible for the expression of Mg-independent acid and neutral phosphatases, but it is not required for the expression of alkaline phosphatases.
Departamento de Microbiología, Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, Spain.
Juana
J
Carrero-Lérida
Aurelio
A
Moraleda-Muñoz
Raquel
R
García-Hernández
Juana
J
Pérez
José
J
Muñoz-Dorado
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D005838:Genotype; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development*; D010641:Phenotype; D011487:Protein Conformation; D011494:Protein Kinases / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D014157:Transcription Factors; C061279:PhoR protein, Bacteria; C061064:PhoP protein, Bacteria; D011494:Protein Kinases