Variants of the primate vesicular monoamine transporter-2.
The vesicular monoamine transporter-2 (VMAT2) sequesters monoamine neurotransmitters into vesicles and prevents neurotoxicity. Human or monkey striatum generated three VMAT2 immunoreactive proteins of approximately 75 kDa, approximately 52-55 kDa, and approximately 45 kDa. The approximately 55-kDa band is considered the unglycosylated native protein. Deglycosylation of the VMAT2 from striatum or human VMAT2 expressed in HEK293 cells yielded a approximately 45-kDa, but not a 55-kDa immunoreactive band. We investigated this apparent mismatch between observed molecular size and predicted size.
Department of Psychiatry, Harvard Medical School, New England Primate Research Center, One Pine Hill Drive, Southborough, MA 01772-9102, USA.
Amy K
AK
Jassen
Jeffrey M
JM
Brown
Helen N
HN
Panas
Gregory M
GM
Miller
Danqing
D
Xiao
Bertha K
BK
Madras
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000493:pharmacokinetics; D006031:Glycosylation; D006801:Humans; D008253:Macaca mulatta; D046911:Macromolecular Substances / Q000378:metabolism; D008958:Models, Molecular; D008970:Molecular Weight; D016296:Mutagenesis; D010766:Phosphorylation; D014162:Transfection; D014316:Tritium / Q000493:pharmacokinetics; D050493:Vesicular Monoamine Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D046911:Macromolecular Substances; D050493:Vesicular Monoamine Transport Proteins; D014316:Tritium; D004298:Dopamine