Description of inflammation and cytokine profile at the inoculation site and in heart tissue of mice re-infected with Trypanosoma cruzi vector derived-metacyclic trypomastigotes.
We studied the role of Trypanosoma cruzi reinfection in regard to inflammatory and cytokine response at the inoculation site, lymph node and heart. We reinfected Balb/c mice intradermically into the hind foot-pad with natural infective metacyclic trypomastigotes. They were followed from 24 h to 30 days after the last reinfection. At the inoculation site 24 h after the last re-infection, the infiltrating inflammatory cells increased dramatically with respect to baseline inflammation, reaching maximum infiltrates for the third day. In contrast, parasite DNA was undetectable 24 h after inoculation, despite poor cytokine induction, only IFN-gamma, IL-12 and TGF-beta were noticeable on days 7 and 15, whereas in the lymph nodes draining the inoculation site positive expression of IL-2, IL-4, IL-12 and TGF-beta were found to be induced as soon as 24 h after re-entry of parasite. In the heart, the inflammatory response increased immediately 24 h after re-entry of parasites, reaching its maximum on the 7th day and returning to baseline on day 30. In conclusion, although the inflammatory response is triggered in both compartments by re-entry of parasites, the inflammatory process returns almost to baseline after 30 days, leaving a persistent low-grade inflammation.
Laboratorio Inmunoparasitologia, Instituto Nacional Cardiologia "Ignacio Chávez", México DF.
L
L
Gómez-García
R
R
Alejandre-Aguilar
A
A
Aranda-Fraustro
R
R
Lopez
V M
VM
Monteón
D000818:Animals; D017209:Apoptosis; D014355:Chagas Disease / Q000276:immunology* / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism*; D003872:Dermatitis / Q000469:parasitology; D005260:Female; D020287:In Situ Nick-End Labeling; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000276:immunology* / Q000473:pathology*; D018512:Parasitemia; D012867:Skin / Q000276:immunology / Q000473:pathology*
D016207:Cytokines