Development of a headspace gas chromatography method to determine residual aliphatic amines in oligonucleotides.
A headspace gas chromatographic method has been developed for analysis of aliphatic amines in oligonucleotides. The linearity was improved by including ammonia in the sample vials. Also, carryover of longer chain primary amines, hexylamine and octylamine, was substantially reduced by cleaning the injection syringe with ammonia and water vapor between sample injections.
Analytical Sciences Laboratory, The Dow Chemical Company, 1897 Building, Midland, MI 48667, USA. vonwaldr@tir.com
Grant
G
Von Wald
Dave
D
Albers
Larry
L
Nicholson
Marsha
M
Langhorst
Bruce
B
Bell
D000588:Amines / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000588:Amines; D009841:Oligonucleotides