[Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique].
OBJECTIVE
To screen the antibibody mimic peptide binding to hepatocellular carcinoma (HCC) from the circular 7-mer peptide libraries.


METHODS
The enrichment of phage was achieved by repetitious biopanning. The affinity of peptide was evaluated by ELISA, and the binding site of the peptide was analysed by the competition ELISA.


RESULTS
The output of phage gradually rose along with increasing of biopanning times. The obtained circular 7-mer peptide showed good binding activity to hepatocellular carcinoma cell lines SMMC-7721 and BEL-7402 (P<0.05), and its affinity to SMMC-7721 cells was superior to that of BEL-7402 (P<0.05). At the level of alpha=0.01, the screened 7-mer peptide monoclonal stocks could obviously compete with scFv to bind SMMC-7721 cells (0.005<P<0.01). As the concentration of scFv gradually fell, the competition inhibition rate dropped accordingly. Sequence analysis showed that the sequences of 7-mer positive monoclonal stocks were identical.


CONCLUSIONS
The peptide obtained through biopanning was specific and effective, which may provide further support for target therapy of HCC.
The State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Han-ping
HP
Li
Yu-ling
YL
Zheng
Hong
H
Zhou
Zheng-ying
ZY
Xiong
Yong-qiang
YQ
Jiang
D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity; D001483:Base Sequence; D001667:Binding, Competitive; D032701:Biomimetics; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D045744:Cell Line, Tumor; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000473:pathology; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000032:analysis / Q000276:immunology*; D011485:Protein Binding
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D010456:Peptides, Cyclic