Phospholipase D and its application in biocatalysis.
Phospholipase D (PLD) from plants or microorganisms is used as biocatalyst in the transformation of phospholipids and phospholipid analogs in both laboratory and industrial scale. In recent years the elucidation of the primary structure of many PLDs from several sources, as well as the resolution of the first crystal structure of a microbial PLD, have yielded new insights into the structural basis and the catalytic mechanism of this catalyst. This review summarizes some new results of PLD research in the light of application.
Department of Biochemistry/Biotechnology, Martin-Luther University Halle-Wittenberg, D-06120, Halle, Germany. ulbrich-hofmann@biochemtech.uni-halle.de
Renate
R
Ulbrich-Hofmann
Alexandra
A
Lerchner
Marek
M
Oblozinsky
Lydia
L
Bezakova
D002384:Catalysis; D020404:Glycerophospholipids / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008956:Models, Chemical; D010739:Phospholipase D / Q000737:chemistry / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D017434:Protein Structure, Tertiary; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D020404:Glycerophospholipids; D010739:Phospholipase D