Inflammatory reaction following cataract surgery and implantation of acrylic intraocular lens in rabbits with endotoxin-induced uveitis.
OBJECTIVE
To investigate whether inflammatory responses are more severe in uveitic eyes than nonuveitic eyes when acrylic intraocular lens (IOL) is implanted after cataract surgery.


METHODS
Clear lens removal (phacoemulsification and aspiration) was conducted and the hydrophobic acrylic IOL (AR40e, AMO) was implanted in adult albino rabbits. Just after the operation, rabbits were divided into two groups. One group (nine rabbits) received intravitreal injection of lipopolysaccharide (LPS, 200 ng/10 microl) into both eyes to induce endotoxin-induced uveitis (EIU) and the other group (nine rabbits) received intravitreal injection of phosphate-buffered saline (PBS, 10 microl) into both eyes as the control. Aqueous humour (AH) and IOLs were harvested 1, 3 , and 7 days after the intravitreal injection. The infiltrating cell number in AH was counted and the protein concentration of AH was measured. IOLs were evaluated morphologically.


RESULTS
At 1 day after intravitreal injection, both the infiltrating cell number in AH and protein concentration of AH were significantly higher in the LPS-injected group than in the PBS-injected group. Similarly, more inflammatory cells attached to the surfaces of the IOLs in the LPS-injected group. However, 7 days later, inflammatory reactions subsided and no clear differences in any of the parameters examined were observed between the two groups.


CONCLUSIONS
At 7 days after the operation, inflammatory reactions in eyes implanted with the hydrophobic acrylic IOLs were similar in uveitic eyes and nonuveitic eyes. The data suggest that the hydrophobic acrylic IOLs may be suitable for patients with uveitis.
Department of Ophthalmology, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Japan.
Y
Y
Koura
A
A
Fukushima
K
K
Nishino
W
W
Ishida
T
T
Nakakuki
M
M
Sento
K
K
Yamazoe
T
T
Yamaguchi
T
T
Misyoshi
H
H
Ueno
D000180:Acrylic Resins; D000208:Acute Disease; D000818:Animals; D001082:Aqueous Humor / Q000166:cytology; D001672:Biocompatible Materials; D002448:Cell Adhesion; D009877:Endophthalmitis / Q000209:etiology; D019654:Lens Implantation, Intraocular / Q000009:adverse effects*; D007910:Lenses, Intraocular; D008070:Lipopolysaccharides; D018918:Phacoemulsification / Q000009:adverse effects*; D011817:Rabbits; D013499:Surface Properties; D014605:Uveitis / Q000150:complications* / Q000473:pathology
D000180:Acrylic Resins; D001672:Biocompatible Materials; D008070:Lipopolysaccharides