Histatin and lactoferrin derived peptides: antimicrobial properties and effects on mammalian cells.
In order to analyze the clinical potential of two antimicrobial peptides, human lactoferrin 1-11 (hLF1-11) and synthetic histatin analogue Dhvar-5, we measured the killing effect on bacteria, and the potential toxicity on erythrocytes and bone cells. The antimicrobial activity was determined in a killing assay on six strains, including methicillin resistant Staphylococcus Aureus. The effect on human erythrocytes and MC3T3 mouse bone cells was measured with a hemolysis assay and a viability assay, respectively. Both hLF1-11 and Dhvar-5 dose-dependently killed all bacterial strains, starting at concentrations of 6 microg/mL. hLF1-11 had no effect on mammalian cells at concentrations up to 400 microg/mL, but Dhvar-5 induced significant hemolysis (37% at 200 microg/mL) and bone cell death (70% at 400 microg/mL). This indicates that both peptides are able to kill various resistant and non-resistant bacteria, but Dhvar-5 may exert a cytotoxic effect on host cells at higher concentrations.
Orthopedic Surgery, VU Medical Center, PO box 7057, 1007 MB Amsterdam, The Netherlands. h.stallmann@vumc.nl
Hein P
HP
Stallmann
Chris
C
Faber
Antonius L J J
AL
Bronckers
Jolanda M A
JM
de Blieck-Hogervorst
Carlo P J M
CP
Brouwer
Arie V Nieuw
AV
Amerongen
Paul I J M
PI
Wuisman
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D054808:Histatins; D006801:Humans; D007781:Lactoferrin; D016106:Methicillin Resistance; D051379:Mice; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000494:pharmacology*; D012471:Salivary Proteins and Peptides / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000254:growth & development*
D000890:Anti-Infective Agents; C504850:Dhvar-5; D054808:Histatins; D010446:Peptide Fragments; D012471:Salivary Proteins and Peptides; C466658:lactoferrin (1-11), human; D007781:Lactoferrin