[Study on ultrasonic extraction technics of oleanolic acid in Akebia trifoliata].
OBJECTIVE
To optimize the ultrasonic extraction condition for oleanolic acid from Akebia trifoliata.


METHODS
According to the orthogonal design L9 (3(4)), the optimum ultrasonic extraction technique was sought through experimental investigation, and was compared with the routine extraction technics. The contents of oleanclic acid were determined by UV-spectrophotometry.


RESULTS
The order of factors to affect the oleanolic acid extraction was extraction time > ethanol concentration > ultrasonic power > ration of interval time to ultrasonic time. The optimum ultrasonic extractions were A2B3C1D2.


CONCLUSIONS
Compared with traditional extraction, ultrasonic method can save time, be easy to operate, improve extraction rates and need no heating.
Sichuan Agriculture University, Ya'an 625014.
Weiguo
W
Liu
Wenyu
W
Yang
Qiyin
Q
Xiao
D000704:Analysis of Variance; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol / Q000008:administration & dosage; D009828:Oleanolic Acid / Q000032:analysis / Q000302:isolation & purification*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
D004365:Drugs, Chinese Herbal; D000431:Ethanol; D009828:Oleanolic Acid