Expression of interleukin-8 and monocyte chemotactic protein-1 in adenomyosis.
BACKGROUND
To clarify the inflammatory nature of adenomyosis, we aimed to investigate the expression of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) by immunohistochemistry to determine their putative role in pathophysiology of adenomyosis.


METHODS
Adenomyosis samples, with their eutopic endometrium, were collected from 30 women undergoing hysterectomy. Endometrium from 27 women without adenomyosis were also collected as a control group. Samples were grouped according to the menstrual cycle phase and examined by immunohistochemistry for IL-8 and MCP-1.


RESULTS
In normal endometrium, secretory phase samples expressed higher levels of epithelial IL-8 than in proliferative phase samples (P = 0.01), and we observed a trend for an increased epithelial MCP-1 expression in the secretory phase samples compared with the proliferative phase samples (P = 0.07). Endometrial samples of women with adenomyosis did not show the same cyclic variation. In the secretory phase, eutopic endometrium of women with adenomyosis expressed lower levels of epithelial IL-8 and MCP-1 compared with normal endometrium (P < 0.05). The expression of epithelial IL-8 and MCP-1 was higher in the adenomyosis foci than the eutopic endometrium (P < 0.05).


CONCLUSIONS
These findings may indicate that an intrinsic abnormality of inflammatory response may be present in eutopic endometrium of women with adenomyosis, and IL-8 and MCP-1 may contribute to the pathophysiology of adenomyosis.
Yale University School of Medicine, Department of Pathology, New Haven, CT 06520, USA.
E Cagnur
EC
Ulukus
Murat
M
Ulukus
Yasemin
Y
Seval
Wenxin
W
Zheng
Aydin
A
Arici
D000328:Adult; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007044:Hysterectomy; D007150:Immunohistochemistry; D007249:Inflammation; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000378:metabolism; D008875:Middle Aged; D012189:Retrospective Studies; D013997:Time Factors
D018932:Chemokine CCL2; D016209:Interleukin-8