A novel function of transcription factor alpha-Pal/NRF-1: increasing neurite outgrowth.
Alpha-Pal/NRF-1 is a critical regulator of the promoter of human IAP/CD47 gene, a gene related to memory formation in rodents. However, its function in neurons was unknown. We found that stable or transient expression of full-length alpha-Pal/NRF-1 in human neuroblastoma IMR-32 cells significantly induced neurite outgrowth and increased the length of neurites both in medium containing 10% fetal bovine serum and in serum-free medium. In contrast, the dominant-negative mutant of alpha-Pal/NRF-1 inhibited the induction and extension of neurites. Ectopic expression of full-length alpha-Pal/NRF-1 also increased the induction of neurite outgrowth in primary mouse cortical neurons. The IAP antisense cDNA significantly inhibited the increase of neurite outgrowth by alpha-Pal/NRF-1. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene.
Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, Tainan 701, Taiwan.
Wen-Teng
WT
Chang
Hsiun-ing
HI
Chen
Rong-Jing
RJ
Chiou
Chen-Yun
CY
Chen
A-Min
AM
Huang
D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism* / Q000473:pathology*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology*; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D011994:Recombinant Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C088388:alpha-Pal transcription factor; C030193:immunosuppressive acidic protein