Capillary electrophoresis of glutathione to monitor oxidative stress and response to antioxidant treatments in an animal model.
Glutathione plays a central role in metabolism and antioxidant defence. Several factors can influence the analytical efficiency and rapidity of the quantitative determination of glutathione. Procedures in sample pre-treatment have been compared in order to minimize analytical errors. Capillary electrophoresis has been chosen as a more adequate technique for obtaining a rapid and simple method for glutathione and glutathione disulfide determination in the blood and liver of the rat. The methods, once optimised, have been validated and applied for monitoring the oxidative stress in an animal model, such as the rat made diabetic by streptozotocin injection, when the animals are treated with antioxidants and compared with the corresponding controls.
Sección Química Analítica, Facultad de Farmacia, Universidad San Pablo-CEU, Urbanización Montepríncipe, Madrid, Spain.
N
N
Maeso
D
D
García-Martínez
F J
FJ
Rupérez
A
A
Cifuentes
C
C
Barbas
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D005978:Glutathione / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D019803:Glutathione Disulfide / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D005978:Glutathione; D001205:Ascorbic Acid; D019803:Glutathione Disulfide