[Effect of exogenous beta-carotene and illumination on sclerotial differentiation and carotenoid yield of Penicillium sp. PT95].
The influence of exogenous beta-carotene and illumination on sclerotial differentiation and carotenoid yield of Penicillium sp. PT95 was studied. The results showed that in the medium supplemented with exogenous beta-carotene, the time of both exudates and sclerotial initials of PT95 strain were delayed. The higher the concentration of exogenous beta-carotene was, the longer the time of delay. But the time of sclerotial maturation was not changed. On the other hand, the exogenous beta-carotene also caused a concentration-dependent reduction of both lipid peroxidation and content of carotenoid in sclerotia. The growth condition at high (light) oxidative stress favored the sclerotial differentiation and pigment accumulation of PT95. The sclerotia biomass and carotenoid yield was 1.18 and 2.01 fold higher respectively at high than at low (dark) oxidative stress. These data revealed that in order to attain higher sclerotia biomass and pigment yield, the strain PT95 should be grown under high oxidative stress and in the absence of antioxidants.
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China. maomaozx@126.com
Wen-jing
WJ
Zhao
Yu-ying
YY
Gao
Jian-rong
JR
Han
D018533:Biomass; D002338:Carotenoids / Q000378:metabolism*; D003470:Culture Media; D008027:Light; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D019207:beta Carotene / Q000494:pharmacology*
D003470:Culture Media; D019207:beta Carotene; D002338:Carotenoids