Assay for uric acid level in rat striatum by a reagentless biosensor based on functionalized multi-wall carbon nanotubes with tin oxide.
A novel reagentless amperometric uric acid biosensor based on functionalized multi-wall carbon nanotubes (MWCNTs) with tin oxide (SnO2) nanoparticles has been developed. This was successfully applied to assay uric acid levels from an in vivo microdialysis sampling. Compared with unfunctionalized or traditional carboxylic acid (-COOH)-functionalized MWCNTs, the MWCNTs-SnO2 electrode exhibited higher electrocatalytic oxidation to uric acid. Here, MWCNTs-SnO2 may act as an efficient promoter, and the system exhibited a linear dependence on the uric acid concentration over the range from 1.0 x 10(-7) to 5.0 x 10(-4) mol L(-1). In addition, there was little ascorbic acid interference. The high sensitivity of the MWCNTs-SnO2 modified enzyme electrode enabled the monitoring of trace levels of uric acid in dialysate samples in rat striatum.
Zhang
Fen-Fen
FF
Department of Chemistry, East China Normal University, Shanghai 200062, China. fenfenzhang@yahoo.com.cn
Wang
Xiao-Li
XL
Li
Chen-Xin
CX
Li
Xiao-Hua
XH
Wan
Qiao
Q
Xian
Yue-Zhong
YZ
Jin
Li-Tong
LT
Yamamoto
Katsunobu
K
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D003342:Corpus Striatum / Q000737:chemistry*; D004563:Electrochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds / Q000737:chemistry*; D014503:Urate Oxidase / Q000737:chemistry*; D014527:Uric Acid / Q000032:analysis*
D037742:Nanotubes, Carbon; D017971:Tin Compounds; D014527:Uric Acid; D014503:Urate Oxidase; C045358:stannic oxide