The best control for the specificity of RNAi.
RNA interference (RNAi) is revolutionizing functional genomics. However, there are several reasons to be concerned about the specificity and off-target effects of this technique. A recent paper by Kittler et al. describes a straightforward way to validate RNAi specificity, which exploits the increasing availability of bacterial artificial chromosome (BAC) clone resources. Genetic rescue of the RNAi phenotype by BAC transgenesis is the best control yet described for specificity, and has further implications for reverse genetics.
Sarov
Mihail
M
Genomics, BioInnovationZentrum, The University of Technology, Dresden, Am Tatzberg 47, 01307 Dresden, Germany.
Stewart
A Francis
AF
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D005818:Genetic Engineering; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D019076:Transgenes
D034741:RNA, Small Interfering