Creating new specific ligand-receptor pairs for transgene regulation.
The creation of specifically matched ligand-receptor pairs that are orthogonal to naturally present interacting pairs is essential for the development of small molecule-regulated gene expression systems for biotechnological applications. However, for many years this task has represented a significant challenge for synthetic chemists and protein engineers. Recently, Doyle and colleagues demonstrated that highly specific ligand-receptor pairs can be engineered in a rapid fashion by creating large libraries of protein variants and applying a selection scheme to identify variants with improved activation by the target synthetic ligand.
Chockalingam
Karuppiah
K
Department of Chemical Engineering and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801, USA.
Zhao
Huimin
H
D001665:Binding Sites; D001671:Biochemistry / Q000379:methods; D001709:Biotechnology / Q000379:methods / Q000639:trends; D005786:Gene Expression Regulation; D008024:Ligands; D015202:Protein Engineering / Q000379:methods*; D047488:Retinoid X Receptors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
D008024:Ligands; D047488:Retinoid X Receptors