[Advances in Detection Technologies of in vivo Expression of Bacterial Virulence Gene.].
The interactions between bacterial pathogens and their hosts is complex. To further our understanding ofathe pathogenesisaof bacterial pathogens, it is necessary to identify bacterial virulence genes that are specifically induced in vivo during infection and probe their regulation in vivo. Toward this end, several technologies, such as in vivo expression technology (IVET), signature-tagged mutagenesis (STM), differential fluorescence induction (DFI), genomic analysis and mapping by in vitro transposition (GAMBIT) and in vivo induced antigen technology (IVIAT), have been developed. The purpose of this reviewais to update the reader on the many advances of these technologies, and to discuss their advantages and disadvantages.
Chen
Shi-Yong
SY
Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China, Email: Dragonchsy@yahoo.com.cn.
Mo
Zhao-Lan
ZL
Zhang
Zhen-Dong
ZD
Zou
Yu-Xia
YX
Xu
Yong-Li
YL
Zhang
Pei-Jun
PJ
D005798:Genes, Bacterial; D014774:Virulence