[Influence of pinacidil on the myocardial mitochondrial structure and the respiratory function in scalded rats].
OBJECTIVE
To investigate the influence of pinacidil preconditioning on the protection of the structure and respiratory function of injured myocardial mitochondria in scalded rats.


METHODS
Seventy-five healthy Wistar rats, weighed 250 approximately 300 g, were randomly divided into three groups: i.e. control (n = 9, with intraperitoneal injection of 50 microg/kg isotonic saline), scald (n = 33, with 30% TBSA full thickness scald) and pre-conditioning (n = 33, with same extent of scald injury after intraperitoneal injection of 50 microg/kg pinacidil) groups. Mitochondrial ultrastructure was observed by transmission electron microscope. The mitochondrial respiratory function, the MDA content and the superoxide anion level were determined with corresponding methods.


RESULTS
The degree of injury to rat myocardial mitochondria in pre-conditioning group was less intensive than that in scald group (P < 0.05 or 0.01). The respiratory control rate in pre-conditioning group was obviously higher than that in scald group (P < 0.05), and the contents of MDA and superoxide anion in pre-conditioning group were markedly lower than those in scald group (P < 0.05 or 0.01), as evidenced by their contents at 3 post scalding hours (0.60 +/- 0.09 micromol/g and 0.127 +/- 0.020) were obviously lower than those in scald group (0.83 +/- 0.07 micromol/g and 0.169 +/- 0.015) (P < 0.01).


CONCLUSIONS
Pinacidil preconditioning was beneficial in the protection of myocardial mitochondria in scalded rats, and it might be related to the pre-opening of potassium channel which was sensitive to mitochondrial ATP.
Department of Geriatrics, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Nian-yin
NY
Peng
Hong
H
Zhou
Liang-yi
LY
Si
D000818:Animals; D002056:Burns / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D019069:Cell Respiration / Q000187:drug effects; D004195:Disease Models, Animal; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000473:pathology; D020110:Pinacidil / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000032:analysis
D013481:Superoxides; D020110:Pinacidil