BAF complex is closely related to and interacts with NF1/CTF and RNA polymerase II in gene transcriptional activation.
Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP II) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP II in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the ConA-stimulated, but not the resting, T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.
Zhao
Li-Hui
LH
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Ba
Xue-Qing
XQ
Wang
Xiao-Guang
XG
Zhu
Xiao-Juan
XJ
Wang
Li
L
Zeng
Xian-Lu
XL
D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000502:physiology*; D004265:DNA Helicases; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019169:Jurkat Cells; D051379:Mice; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000502:physiology*; D012319:RNA Polymerase II / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
D022762:CCAAT-Enhancer-Binding Proteins; D051839:NFI Transcription Factors; C497647:NFIC protein, human; C497648:Nfic protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II; C084108:SMARCA4 protein, human; C513194:Smarca4 protein, mouse; D004265:DNA Helicases