[Expression of gastrin in human gastric cancer cell line and effect of anti-gastrin mAb on the cell growth].
OBJECTIVE
To investigate the expression of gastrin in human gastric cancer cell line SGC-7901 and the effects of gastrin-17 and anti-gastrin mAb on its growth.


METHODS
The expression of gastrin was determined by immunohistochemistry with anti-gastrin mAb prepared by our group. In a series of experiments, the growth of SGC-7901 cells was evaluated by MTT assay on cells grown in serum-free medium and treated with gastrin-17 and/or anti-gastrin mAb.


RESULTS
Immunohistochemical examination of SGC-7901 cells revealed a specific gastrin immunoreactivity. Gastrin-17 significantly stimulated cell growth at the concentrations of 1 x 10(-9) mol/L approximately 1 x 10(-5) mol/L in a dose-dependent manner. The growth of SGC-7901 cells treated with anti-gastrin mAb, either alone or in combination with gastrin-17 (1 x 10(-7) mol/L), was significantly inhibited.


CONCLUSIONS
Growth of human gastric cancer cells SGC-7901 can be stimulated in an autocrine fashion. The gastrin-stimulated growth of gastric cancer cells can be blocked by anti-gastrin mAb bound specifically with gastrin. Further study on the significance of anti-gastrin mAb in designing immunotherapy targeting to gastrin or gastrin receptor is warranted.
Immunology Department, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. xiawang1971@sohu.com
Xia
X
Wang
Da-peng
DP
Wei
Mei-ying
MY
Cai
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005755:Gastrins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
D000911:Antibodies, Monoclonal; D005755:Gastrins; D043887:Receptor, Cholecystokinin B; C013550:gastrin 17