(6-4)-photolyase activity requires a charge shift reaction.
A model compound containing a thymine oxetane moiety linked to a flavin chromophore was investigated regarding (6-4)-photolyase activity. The need for a charge shift reaction was demonstrated by a detailed pH-dependent kinetic analysis.
Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, D-37077 Göttingen, Germany.
Thorsten
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Stafforst
Ulf
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Diederichsen
D002384:Catalysis; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry*; D005415:Flavins / Q000737:chemistry*; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular
D005415:Flavins; D006575:Heterocyclic Compounds, 3-Ring; D004255:Deoxyribodipyrimidine Photo-Lyase