[Expression of keratinocyte basement membrane related genes during the process of re-epithelialization of burn wound in scalded rats].
OBJECTIVE
To study the expression of keratinocyte (KC) basement membrane (BM) related genes during the process of re-epithelialization of burn wound in scalded rats with cDNA microarray technique.


METHODS
Twenty-four SD rats were inflicted with deep partial thickness scald with an area of 45 cm(2) on the back, and they were randomly divided into A [3 postscald day (PSD)], B (10 PSD), C (14 PSD) and D (re-epithelialization complete day) groups, with 6 rats in each group. Tissue samples were harvested from 1 cm of wound margin on 3, 10 and 14 PSD. On the re-epithelialization complete day, tissue samples were harvested from the center of the wound in D group and digested with enzyme into KC suspensions. Skin samples from the back of 6 uninjured rats were taken as normal control. The differential expression of KC BM related genes during different stages of re-epithelialization was assayed with cDNA microarray.


RESULTS
The expression of laminin (LN) gamma 1 (2.068) and integrin beta8 (2.200) was up-regulated on 3 PSD compared with that in control. The expression of integrin beta1 and LN receptor 1 was up-regulated on 10 and 14 PSD, (2.472 and 2.658), while that of integrin beta2 and beta1 (0.419 and 0.462) down-regulated on 10 and 14 PSD, and the expression of type IV collagen alpha1 and alpha3 was up-regulated during re-epithelialization.


CONCLUSIONS
The expression of integrin beta1, LN gamma 1, LN receptor 1, type IV collagen alpha1 and alpha3 genes were up-regulated during re-epithelialization, which might be beneficial to the construction of BM in new skin and the formation of stable conjunction between KC and BM.
Department of Burns, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P.R. China.
Xiong
X
Zhang
Yan
Y
Liu
Zhi
Z
Zhang
Wei-shi
WS
Xu
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D024141:Collagen Type IV / Q000235:genetics; D004195:Disease Models, Animal; D004848:Epithelium / Q000378:metabolism; D015870:Gene Expression; D019012:Integrin beta1 / Q000235:genetics; D016023:Integrins / Q000235:genetics; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D007797:Laminin / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012867:Skin / Q000166:cytology; D014945:Wound Healing
D024141:Collagen Type IV; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; C089141:laminin gamma 1