Determination of peroxidase encapsulated in liposomes using homogentisic acid y-lactone chemiluminescence.
Homogentisic acid gamma-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in piodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.
Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan.
Tamio
T
Kamidate
Nobuhito
N
Kikuchi
Akihiko
A
Ishida
Hirofumi
H
Tani
D002138:Calibration; D003902:Detergents; D006713:Homogentisic Acid / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000032:analysis*; D007202:Indicators and Reagents; D007783:Lactones / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D008163:Luminescent Measurements; D008165:Luminol; D017830:Octoxynol
D003902:Detergents; D007202:Indicators and Reagents; D007783:Lactones; D008055:Lipids; D008081:Liposomes; D008165:Luminol; D017830:Octoxynol; D006735:Horseradish Peroxidase; D006713:Homogentisic Acid