[Identification of Vibrio campbellii isolated from cultured pacific oyster].
Four bacterial strains were isolated from cultured pacific oyster (Crassostrea gigia) in September 2003 at coast of Fujian province. Their morphological, physiological and biochemical characteristics and 16S rRNA sequence were analyzed. And the relationship between reproduction of the bacterium and NaCl concentrations, pH and temperature were also determined. The results showed that four strains were gram-negative rods with a single polar flagellum, glucose fermented without gas production, oxidase positive, required sodium ions for growth, no pigment, non-luminescence; They grew well on TCBS-plate as green colonies and were sensitive to the vibriostratic agent O/129. Therefore, it was confirmed that the strains belonged to the genus of Vibrio. The sequence analysis of 16S rRNA gene of SXS1 isolation and comparison with that of other related vibrios- showed that SXS1 was very close to Vibrio campbellii. The similarities was 99%. The distribution of V. campbellii in environment and its relationship to the diseases of aquaculture animals were discussed.
Shen
Xiao-sheng
XS
East China Sea Fisheries Research Institute, Chinese Fisheries Academy of Fishery Science, Shanghai 200090, China. foodsmc98@tom.com
Cai
You-qiong
YQ
Fang
Wen-hong
WH
Gu
Run-run
RR
Gao
Dan-feng
DF
D000818:Animals; D049896:Crassostrea / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S