[Optimization of parameters of exogene transfection of bovine fetal fibroblasts in vitro mediated by liposome].
pEGFP-C1 eucaryon expression vector was successfully transfected by liposome into bovine fetal fibroblasts. We investigated the effect of parameter such as the dose of DNA and liposome,number of cell transfected and exposure time of the cell to the DNA-liposome complexes. It was indicated that GFP (green fluorescent protein) expression was enhanced as the dose of DNA and liposome increased and on decline as the exposure time was prolonged. The improvement of transfection efficiency depent on the suitable cell number.
The Research Center for Laboratory Animal Science of Inner Mongolia University, The Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Huhhot 010021, China.
Yang
Y
Li
Kai-feng
KF
Wu
Xu-dong
XD
Guo
Ji-tong
JT
Guo
Shor-gan
SG
Bou