A putative early response of antifungal Bacillus lentimorbus WJ5 against the plant pathogenic fungus, Colletotrichum gloeosporioides, analyzed by a DNA microarray.
The global RNA transcription profiles of Bacillus lentimorbus WJ5 under an in vitro co-culture with Colletotrichum gloeosporioides were analyzed in order to study the antagonistic bacteria-fungi interactions. Using a filter membrane system, B. lentimorbus WJ5 was exposed to the spores of C. gloeosporioides at the late exponential stage. The transcription profiles of the B. lentimorbus WJ5, both with and without a challenge from C. gloeosporioides, were analyzed using custom DNA chips containing 2,000 genome fragments. A total of 337 genes were expressed, with 87 and 47 up- and down-regulated, respectively. Of these, 12 genes, which were involved in central carbon metabolisms, and 7 from minor catabolism were relatively highly up-regulated (> 10 fold) and down-regulated (< 0.2 fold), respectively. Nine genes, which were thought to be related to the antifungal activity, were also up-regulated, but their levels were not so high (2.0 - 9.7 folds). From the results, during the early stage of the co-culture of B. lentimorbus WJ5 and C. gloeosporioides, nutrient competition seemed to occur; therefore, the genes from central carbon metabolisms could be up-regulated, while those from minor catabolism could be down-regulated.
Lee
Young-Keun
YK
Radiation Application Research Division, Korea Atomic Energy Research Institute, Sinjeong, Jeongeup 580-185, Republic of Korea. yklee@kaeri.re.kr
Jang
Yu-Sin
YS
Chang
Hwa-Hyoung
HH
Hyung
Seok Won
SW
Chung
Hye-Young
HY
D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology; D020231:Colletotrichum / Q000254:growth & development* / Q000472:pathogenicity; D003470:Culture Media; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000382:microbiology*
D000935:Antifungal Agents; D001426:Bacterial Proteins; D003470:Culture Media