Development of a new sampling medium for bioaerosols.
OBJECTIVE
To develop a new sampling medium for detecting of bioaerosols.


METHODS
The sampling media were tested by using Escherichia coli, Staphylococcus aureus and Serratia marcescens under static and active conditions, preliminary applications were performed using AGI-10 and high volume sampler.


RESULTS
The average recovery rates were raised to 24.7%, 58.2%, 40.5%, 44.1%, 20.5%, and 15.4%, respectively in six consecutive experiments under static condition for 60 min at room temperature. Four kinds of sampling media were singled out after static experiments, which were referred to as "samplutions" PD1, PX2, TD1, and TX2, respectively. Under the active condition, the protective efficacy of PD1, PX2, TD1, and TX2 was 226% (153/47), 553% (111/17), 150% (120/48), and 268% (419/114), respectively.


CONCLUSIONS
The samplutions have some effects on the subsequent nucleic acid detection, which could be avoided by employing standard nucleic acid extraction procedure. The newly developed samplution can be applied to the detection of bioaerosols.
Institute of Microbiology and Epidemiology, 20 Dongdajie, Fengtai District, Beijing 100071, China.
Jun-Hui
JH
Zhai
Mei-Ling
ML
Chen
Xiu-Zhi
XZ
Xu
Zhen-Hai
ZH
Sun
Yu
Y
Zhou
Feng-Xiang
FX
Che
Rui-Fu
RF
Yang
D000336:Aerosols / Q000032:analysis; D000391:Air Microbiology; D000393:Air Pollutants / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification; D009696:Nucleic Acids / Q000302:isolation & purification; D012494:Sampling Studies; D012706:Serratia marcescens / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification
D000336:Aerosols; D000393:Air Pollutants; D009696:Nucleic Acids