[Effects of hypothermia on antioxidant capacity of rat testes after testicular torsion].
OBJECTIVE
To investigate the effects of hypothermia on the antioxidant capacity of rat testes after testicular torsion.


METHODS
Twenty-four healthy pubertal male Sprague-Dawley rats were randomly divided into three groups of equal number: Group A (torsion) , Group B (torsion + hypothermia) and Group C (control). The animals were submitted to unilateral 720 degrees testicular torsion, and underwent detorsion two hours later. Fourteen days later, the total antioxidant capacity (T-AOC) and the level of malonic diethylaldehyde(MDA) were detected with spectrophotometer and histological changes were observed by light microscope.


RESULTS
The T-AOC of Group B was significantly greater than that of Group A (P < 0.01), but less than that of Group C (P < 0.01). The MDA level of Group B was lower than that of Group A (P < 0.01), but higher than that of Group C (P < 0.05).


CONCLUSIONS
Hypothermia can restrain the production of oxygen free radicals following testicular torsion/detorsion in rats, which in turn can inhibit lipid peroxidation and increase the survivability of the torsional testis.
Chen
Hai-Tao
HT
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Zheng
Xin-Min
XM
Yang
Zhi-Wei
ZW
Zheng
Hang
H
Li
Shi-Wen
SW
Hu
Li-Quan
LQ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D007036:Hypothermia, Induced; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000473:pathology / Q000503:physiopathology*; D013737:Testis / Q000473:pathology
D000975:Antioxidants; D008315:Malondialdehyde