Structural basis of interaction between protein tyrosine phosphatase PCP-2 and beta-catenin.
PCP-2 is a member of receptor-like protein tyrosine phosphatase of the MAM domain family. To investigate which part of PCP-2 was involved in its interaction with beta-catenin, we constructed various deletion mutants of PCP-2. These PCP-2 mutants and wild-type PCP-2 were co-transfected into BHK-21 cells with beta-catenin individually. An in vivo binding assay revealed that the expression of wild-type PCP-2, PCP-2 deltaC1C2 (deleted PCP-2 without both PTP domains) and PCP-2 deltaC2 (deleted PCP-2 without the second PTP domain) could be immunoprecipitated by anti-catenin antibody in every co-transfection, but PCP-2 EXT (deleted PCP-2 without the juxtamembrane region and both PTP domains) was missing, which implied that PCP-2 and beta-catenin could associate directly and the juxtamembrane region in PCP-2 was sufficient for the process.
International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
Yaqin
Y
He
Hexin
H
Yan
Hui
H
Dong
Peng
P
Zhang
Liang
L
Tang
Xiuhua
X
Qiu
Mengchao
M
Wu
Hongyang
H
Wang
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D011233:Precipitin Tests; D017027:Protein Tyrosine Phosphatases / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry* / Q000378:metabolism*; D014162:Transfection; D051176:beta Catenin
D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin; D017027:Protein Tyrosine Phosphatases