Why doesn't nicotinic ACh receptor immunoreactivity knock out?
Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha7 nicotinic ACh receptors. These data highlight the conundrum facing histologists--how 'real' is the labelling they see?
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK. i.w.jones@bath.ac.uk
Ian W
IW
Jones
Susan
S
Wonnacott
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D018345:Mice, Knockout / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000172:deficiency / Q000276:immunology / Q000378:metabolism; D064569:alpha7 Nicotinic Acetylcholine Receptor
D000906:Antibodies; C579166:Chrna7 protein, mouse; D016056:Immunodominant Epitopes; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor