Quantification of C13orf19/P38IP mRNA expression by quantitative real-time PCR in patients with urological malignancies.
Quantitative real-time PCR was performed for C13orf19, a gene located on chromosome 13q and previously described to be down-regulated in prostate carcinoma, on different cancer cell lines, on matched prostate tissues from 61 patients with prostate carcinoma and on matched kidney tissues from 23 patients with renal clear cell carcinoma. All data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), porphobilinogen deaminase (PBGD) and hypoxanthine guanine phosphoribosyl transferase (HPRT) mRNA expression. A C13orf19 quantitative PCR (QPCR) showed the mRNA to be down-regulated in matched prostate tissues (P=0.007 and lower, paired Student's t-test). However, an at least 1.5-fold C13orf19 mRNA downregulation was observed in samples from 28 patients (46%) and an at least 1.5-fold upregulation was observed in samples from 17 patients (28%). In contrast, C13orf19 mRNA alterations in expression seemed to be random events in kidney cancers.
Department of Urology, Technical University of Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. uta.schmidt2@mailbox.tu-dresden.de
Uta
U
Schmidt
Susanne
S
Fuessel
Michael
M
Haase
Kai
K
Kraemer
Axel
A
Meye
Manfred P
MP
Wirth
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics; D045744:Cell Line, Tumor; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics; D008297:Male; D016366:Open Reading Frames / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D014571:Urologic Neoplasms / Q000235:genetics*
D012333:RNA, Messenger; C502592:SUPT20H protein, human; D014157:Transcription Factors