A novel function of transcription factor alpha-Pal/NRF-1: increasing neurite outgrowth.
Alpha-Pal/NRF-1 is a critical regulator of the promoter of human IAP/CD47 gene, a gene related to memory formation in rodents. However, its function in neurons was unknown. We found that stable or transient expression of full-length alpha-Pal/NRF-1 in human neuroblastoma IMR-32 cells significantly induced neurite outgrowth and increased the length of neurites both in medium containing 10% fetal bovine serum and in serum-free medium. In contrast, the dominant-negative mutant of alpha-Pal/NRF-1 inhibited the induction and extension of neurites. Ectopic expression of full-length alpha-Pal/NRF-1 also increased the induction of neurite outgrowth in primary mouse cortical neurons. The IAP antisense cDNA significantly inhibited the increase of neurite outgrowth by alpha-Pal/NRF-1. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene.
Chang
Wen-Teng
WT
Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, Tainan 701, Taiwan.
Chen
Hsiun-ing
HI
Chiou
Rong-Jing
RJ
Chen
Chen-Yun
CY
Huang
A-Min
AM
D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism* / Q000473:pathology*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology*; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D011994:Recombinant Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C088388:alpha-Pal transcription factor; C030193:immunosuppressive acidic protein