Comparative analysis of the pig BAC sequence involved in the regulation of myostatin gene.
Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. In order to study its expression and regulatory mechanism deeply, we have presented a comparative analysis of about 170-kb pig BAC sequence containing the myostatin gene among pig, human and mouse. The genomic region is characterized by high interspersed repeats and low G+C content. As for the myostatin gene, a higher sequence similarity is found between human and pig than between these species and the mouse. One striking feature is that the structure of two TATA-boxes in the nearby downstream of CCAAT-box is identified in the promoter. Further analysis reveals that the TATA-box1 is responsible for the transcription in pig and human, but the TATA-box2 acts on the transcription in mouse. The other interesting feature is that two polyadenylation signal sequences (AATAAA) exist in 3'UTR of the pig myostatin gene. Moreover, a large number of potential transcription factor-binding sites are also identified in evolutionary conserved regions (ECRs), which may be associated with the regulation of myostatin. Many putative transcription factors play an important role in the muscle development, and the complex interaction between myostatin and these factors may be required for proper muscle development.
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Zhengquan
Z
Yu
Yan
Y
Li
Qingyong
Q
Meng
Jing
J
Yuan
Zhihui
Z
Zhao
Wei
W
Li
Xiaoxiang
X
Hu
Bingxue
B
Yan
Baoliang
B
Fan
Shuyang
S
Yu
Ning
N
Li
D024509:3' Flanking Region; D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018899:CpG Islands; D019143:Evolution, Molecular; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D055435:Myostatin; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine; D016385:TATA Box; D020080:Tandem Repeat Sequences; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*
D020413:3' Untranslated Regions; C525911:MSTN protein, human; C525912:Mstn protein, mouse; D055435:Myostatin; D014157:Transcription Factors; D016212:Transforming Growth Factor beta