Long-term delivery of superoxide dismutase and catalase entrapped in poly(lactide-co-glycolide) microspheres: in vitro effects on isolated neonatal porcine pancreatic cell clusters.
To counterbalance the restricted availability of pancreatic islet tissue for transplant in Type 1 Diabetes Mellitus (T1DM), new methods to provide viable and functional islet cells need to be established. We report on our approach to enhance in vitro viability and function of isolated neonatal pancreatic porcine cell clusters (NPCCs) by co-culturing them with PLGA microsphere entrapped, slowly release superoxide dismutase and catalase. These powerful antioxidizing agents were shown to significantly improve morphology, viability and function, as assessed by microscopy, molecular, biochemical and functional studies, of the incubated NPCCs, as compared to control. Preliminarily, in vitro exposure of isolated NPCCs to slow release microsphere-embedded SOD and CAT could permit or contribute to overcome hurdles associated with scarcity in islet tissue procurement for transplant in T1DM.
Department of Chemistry and Technology of Drugs, Faculty of Pharmacy, University of Perugia, Via del Liceo 1, Perugia 06123, Italy. eureka@unipg.it
Stefano
S
Giovagnoli
Giovanni
G
Luca
Ivan
I
Casaburi
Paolo
P
Blasi
Giacomo
G
Macchiarulo
Maurizio
M
Ricci
Mario
M
Calvitti
Giuseppe
G
Basta
Riccardo
R
Calafiore
Carlo
C
Rossi
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism* / Q000494:pharmacology / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D003692:Delayed-Action Preparations / Q000378:metabolism* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010179:Pancreas / Q000166:cytology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism* / Q000494:pharmacology / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors
D000975:Antioxidants; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002374:Catalase; D013482:Superoxide Dismutase; C018506:diacetylfluorescein