A new cancer diagnostic system based on a CDK profiling technology.
A series of molecular pathological investigations of the molecules that stimulate the cyclin dependent kinases (CDK1, 2, 4, and 6) have led to enormous accumulation of knowledge of the clinical significance of these molecules for cancer diagnosis. However, the molecules have yet to be applied to clinical cancer diagnosis, as there is no available technology for application of the knowledge in a clinical setting. We hypothesized that the direct measurement of CDK activities and expressions (CDK profiling) might produce clinically relevant values for the diagnosis. This study investigated the clinical relevance of CDK profiling in gastrointestinal carcinoma tissues by using originally developed expression and activity analysis methods. We have established novel methods and an apparatus for analyzing the expression and activities of the CDK molecules in lysate of tumor tissue in a clinical setting, and examined 30 surgically dissected gastrointestinal carcinomas and corresponding normal mucosal specimens. We demonstrate here that remarkably elevated CDK2 activity is evident in more than 70% of carcinoma tissues. Moreover, a G1-CDK activity profiling accurately mirrored the differences in proliferation between tumor and normal colonic tissues. Our results suggest that CDK profiling is a potent molecular-clinical approach to complement the conventional pathological diagnosis, and to further assist in the individualized medications.
Ishihara
Hideki
H
Central Research Laboratories, Sysmex Corporation, 4-4-4, Takatsukadai, Nishi-ku, Kobe 651-2271, Japan. Hideki_Ishihara@sysmex.co.jp
Yoshida
Tomokazu
T
Kawasaki
Yuko
Y
Kobayashi
Hironori
H
Yamasaki
Masatoshi
M
Nakayama
Satoshi
S
Miki
Erika
E
Shohmi
Kei-Ichiro
K
Matsushima
Tomoko
T
Tada
Sachiyo
S
Torikoshi
Yasuhiro
Y
Morita
Masakatsu
M
Tamura
Shigeyuki
S
Hino
Yoko
Y
Kamiyama
Jun
J
Sowa
Yoshihiro
Y
Tsuchihashi
Yasunari
Y
Yamagishi
Hisakazu
H
Sakai
Toshiyuki
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000175:diagnosis*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D005260:Female; D005453:Fluorescence; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*
D018844:Cyclin-Dependent Kinases