A general model of reaction kinetics in biological systems.
Dynamic mathematical models in biotechnology require, besides the information about the stoichiometry of the biological reaction system, knowledge about the reaction kinetics. Modulation phenomena like limitation, inhibition and activation occur in different forms of competition with the key enzymes responsible for the respective metabolic reaction steps. The identification of a priori unknown reaction kinetics is often a critical task due to the non-linearity and (over-) parameterization of the model equations introduced to account for all the possible modulation phenomena. The contribution of this paper is to propose a general formulation of reaction kinetics, as an extension of the Michaelis-Menten kinetics, which allows limitation/activation and inhibition effects to be described with a reduced number of parameters. The versatility of the new model structure is demonstrated with application examples.
Haag
J E
JE
Faculté Polytechnique de Mons, Service d'Automatique, 31 Boulevard Dolez, 7000 Mons, Belgium.
Vande Wouwer
A
A
Remy
M
M
D000465:Algorithms; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D003198:Computer Simulation; D005786:Gene Expression Regulation / Q000502:physiology*; D007700:Kinetics; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D008956:Models, Chemical; D009716:Numerical Analysis, Computer-Assisted; D015398:Signal Transduction / Q000502:physiology*; D049490:Systems Biology / Q000379:methods*
D001704:Biopolymers