G protein beta 3 subunit gene variants and essential hypertension in the northern Chinese Han population.
Recently a novel C825T polymorphism in the G protein beta3 subunit gene was identified that showed an association with hypertension in a German population; the results of studies in other populations have been inconsistent. To examine the contribution of GNB3 polymorphisms to the development of hypertension in the northern Chinese Han population, we conducted a case-control study consisting of 501 hypertensive cases and 503 controls using the G(-350)A, C825T and C1429T polymorphisms. Genotypes of samples were determined by PCR and restriction digestion. Single locus analysis showed a significant association between G(-350)A and hypertension (P = 0.01) but no association for C825T or C1429T. The three polymorphisms were in tight linkage disequilibrium (D'=-1 for G(-350)A-C825T, D'= 0.92 for C825T-C1429T) and a total of 7 haplotypes were observed in the entire population. Haplotype A-C-C was found to be significantly related to hypertension (P = 0.032) and A-C-C carriers had a more than two-fold higher risk of hypertension than non-carriers, after adjustment for BMI and glucose. In conclusion, our study suggests that G(-350)A is a potential functional polymorphism that may be related to hypertension, whereas the C825T and C1429T polymorphisms are not associated with hypertension in the northern Chinese Han population.
Li
Biao
B
Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Chaoyang District, Beijing 100101, China.
Ge
Dongliang
D
Wang
Yuelan
Y
Zhao
Weiyan
W
Zhou
Xiaoyang
X
Gu
Dongfeng
D
Chen
Runsheng
R
D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D017354:Point Mutation; D011110:Polymorphism, Genetic; D021122:Protein Subunits / Q000235:genetics*
C454420:G-protein beta3 subunit; D021122:Protein Subunits; D020962:Heterotrimeric GTP-Binding Proteins