Differential intracellular distributions of inositol trisphosphate and ryanodine receptors within and among hematopoietic cells.
To better understand the mechanism(s) of leukocyte Ca(2+) signaling, we have studied the intracellular locations of two Ca(2+)-mobilizing receptors, the inositol 1,4,5-trisphosphate receptor and ryanodine receptor, by immunofluorescence microscopy. Our results show that localization differs not only between receptor classes within a cell, but among leukocyte types as well. We also illustrate the importance of preserving labile cellular filaments in maintaining cell integrity by fixation with the Safiejko-Mroczka and Bell protocol, because conventional fixation methods distort receptor patterns. We suggest that the observed differences influence intracellular Ca(2+) signaling.
Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA.
Andrea J
AJ
Clark
Howard R
HR
Petty
D015220:Calcium Channels / Q000378:metabolism*; D006410:Hematopoiesis; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007962:Leukocytes / Q000378:metabolism* / Q000648:ultrastructure; D008214:Lymphocytes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*
D015220:Calcium Channels; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel