[Effect of glucose on biofilm and the gene ica expression in Staphylococcus epidermidis with different biofilm-forming capability].
The infection of S. epidermidis, an opportunistic human pathogen, depends on biofilm formation, and biofilm formation is closely related to environment. Researches in the thesis focused on two strains of S. epidermidis with different capability of biofilm formation. To find the mechanism of response to environment on biofilm formation, biofilm formation and expression of ica, icaR, AtlE in theses S. epidermidis cultivated in different grow environment and in media with glucose for different time were assayed. Glucose can induce the biofilm formation by inducing ica gene, but the inducing do not need continued ica expression, and other genes also contribute to the regulation; anti-ODN specially binding icaADBC can withstand biofilm inducing from glucose. These results suggest that biofilm formation closely related to growth environment, which is a complex regulation mechanism. Biofilm formation is closely related to bacteria energy metabolism and cell wall synthesis. Some crucial factors in the complex and integrated regulation system have not been known yet.
Key Laboratory of Glycoconyugate Research of Ministry of Public Health, Fudan University, Shanghai 200032, China. jwjin@shmu.edu.cn
Jia-wei
JW
Jin
Li
L
Zhang
Xi-liang
XL
Zha
Hua-lin
HL
Li
Di
D
Qu
D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D016376:Oligonucleotides, Antisense; D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D005947:Glucose