Identification of unique bacterial gene segments from Streptococcus mutans with potential relevance to dental caries by subtraction DNA hybridization.
Using DNA subtractive hybridization, 49 unique gene segments were identified from a strain of Streptococcus mutans that was isolated from a patient with severe early childhood caries (S-ECC). Further hybridization with DNA from other S. mutans strains isolated from both caries-active and caries-free subjects yielded five unique sequences of DNA common to strains associated with S-ECC.
Division of Diagnostics, Infectious Disease, and Heath Promotion, New York University College of Dentistry, 345 E. 24th Street, New York, NY 10010, USA. ds100@nyu.edu
Deepak
D
Saxena
Yihong
Y
Li
Page W
PW
Caufield
D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D003731:Dental Caries / Q000382:microbiology*; D003733:Dental Caries Susceptibility; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013295:Streptococcus mutans / Q000235:genetics* / Q000472:pathogenicity
D004269:DNA, Bacterial