Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA.
The purpose of this study is to evaluate the usefulness of the new real-time PCR COBAS TaqMan 48 analyzer, comparing it to the existing COBAS AMPLICOR HBV MONITOR based on conventional PCR technology. The study used 104 samples from different patients. No differences were found in the sensitivity of the tests. There was an excellent correlation between the sample with a viral load within the dynamic range of the two tests (r = 0.938). The COBAS TaqMan test has a wider linear range, and this fact enables quantifying of the viral load without diluting the sample.
Gordillo
Rafael M
RM
Microbiology and Parasitology Department, Hospital Universitario Reina Sofía, Cordoba, Spain.
Gutiérrez
Juan
J
Casal
Manuel
M
D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase / Q000378:metabolism*; D019562:Viral Load
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase