[Isolation and identification of a strain DN2 degrading nicotine].
A bacterial strain which was able to utilize nicotine as its sole carbon source was isolated from soil in which tobacco had grown at Sanming region in FUjian Province and named as DN2. Upon chemotaxonomic characterization and phylogenetic inference based 16S rDNA analysis, the strain DN2 was identified as a-proteobacteria, Ochrobactrum intermedium. For DN2 degrading nicotine, the optimal pH and temperature is 6.5, 30 degrees C respectively. It can tolerate high-concentration of nicotine up to 4000 mg/L in basal media. Using 500 mg/L nicotine as its sole carbon, the strain was able to degrade 15 mg/L of nicotine per liter per hour and reached its stationary phase in about 36 hours.
Yuan
Yong-jun
YJ
Key Laboratory of Food Processing and Quality Control of Ministry of Agriculture, College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, China. yyj132618@sina.com.cn
Lu
Zhao-xin
ZX
Huang
Li-jin
LJ
Lv
Feng-xia
FX
Bie
Xiao-mei
XM
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D009538:Nicotine / Q000378:metabolism*; D042041:Ochrobactrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D009538:Nicotine