Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb.
Extracellular matrix (ECM) remodeling is achieved by both production and degradation of ECM molecules during bone development. ADAMTS (a disintegrin and metalloprotease with thrombospondin type 1 motifs) constitutes a family of extracellular proteases which are implicated in cleaving the protein versican. The present study was designed to investigate the expression of versican and ADAMTS1, 4, and 5 mRNA during bone development in rat mandibles and hind limbs by RT-PCR and in situ hybridization. Versican was localized by immunohistochemistry. The process of bone development from day 14 postcoitum through week 6 postnatum was divided into the beginning of osteogenesis, woven bone, and lamellar bone stages. Versican protein was abundant in the woven bone matrix, but decreased in the lamellar bone matrix. Versican mRNA was prominent in some osteoblasts with corresponding localization of the cognate protein. The temporal and spatial mRNA expression pattern of ADAMTS1, 4, and 5 was comparable to that of versican. These results suggest that woven bone rich in versican alters into lamellar bone containing little versican during bone development in both mandibles and hind limbs, where some osteoblasts may be involved in production as well as degradation of versican by secreting ADAMTS1, 4, and 5.
Nakamura
Megumi
M
Divisions of Oral Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Sone
Shinya
S
Takahashi
Ichiro
I
Mizoguchi
Itaru
I
Echigo
Seishi
S
Sasano
Yasuyuki
Y
D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000071097:ADAMTS1 Protein; D000071121:ADAMTS4 Protein; D000818:Animals; D000831:Animals, Newborn; D001846:Bone Development; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D006614:Hindlimb / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008334:Mandible / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D011348:Procollagen N-Endopeptidase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053675:Versicans
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C508324:Vcan protein, rat; D053675:Versicans; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486324:Adamts1 protein, rat; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein