Superheated water chromatography-nuclear magnetic resonance spectroscopy of kava lactones.
Three kava lactone constituents of Piper methysticum, namely, kawain, methysticin and desmethoxyyangonin, have been separated and identified by reversed-phase HPLC using superheated deuterium oxide as the mobile phase and on-line 1H-NMR detection. The method avoids the use of organic solvents in the mobile phase and hence interferences in the NMR analysis are minimised.
Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UK.
Orapin
O
Chienthavorn
Roger M
RM
Smith
Ian D
ID
Wilson
Brian
B
Wright
Eva M
EM
Lenz
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D017666:Deuterium Oxide / Q000737:chemistry; D020901:Kava / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification*; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification*
D011714:Pyrans; D011753:Pyrones; D017666:Deuterium Oxide; C076881:methysticin; C110481:yangonin; C011889:kavain