Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.
Satomura
Takenori
T
Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Higashimura-cho, Hiroshima, Japan.
Shimura
Daisuke
D
Asai
Kei
K
Sadaie
Yoshito
Y
Hirooka
Kazutake
K
Fujita
Yasutaro
Y
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D017930:Genes, Reporter; D005838:Genotype; D005972:Glutaminase / Q000235:genetics / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D000071677:Histidine Kinase; D007700:Kinetics; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D015534:Trans-Activators; C037853:glutamine transport proteins; D005973:Glutamine; D011494:Protein Kinases; D000071677:Histidine Kinase; D001616:beta-Galactosidase; D005972:Glutaminase