Possible role of 5'-adenosine triphosphate in synchronization of Ca2+ oscillations in primate luteinizing hormone-releasing hormone neurons.
LHRH neurons derived from the olfactory placode region of monkey embryos exhibit spontaneous intracellular Ca2+ ([Ca2+]i) oscillations that synchronize among LHRH neurons and nonneuronal cells at a frequency similar to pulsatile LHRH release. To understand the mechanism of intercellular communication between LHRH neurons and nonneuronal cells, which leads to synchronization, we examined the possible role of ATP. 1) ATP, not ADP or AMP, stimulated both LHRH release and [Ca2+]i concentration, whereas the ATP-induced [Ca2+]i response was abolished by infusion of apyrase, which hydrolyzes ATP; 2) the ATP-induced [Ca2+]i response occurred in normal (but not low) extracellular Ca2+ and was blocked by the voltage-dependent L-type Ca2+ channel blocker, nifedipine; 3) pharmacological experiments with purinergic receptor agonists and antagonists indicated that the ATP-induced [Ca2+]i response in LHRH neurons was mediated through P2X, but not P2Y, receptors; 4) cloning and sequencing studies suggested that P2X2 and P2X4 transcripts were present in olfactory placode cultures; and 5) P2X2 receptors and P2X4 were expressed in LHRH neurons. The results suggest that ATP may play a role in intercellular communication when LHRH neurons synchronize, and raise the possibility that nonneuronal cells, such as glia, may be a crucial component of the in vivo LHRH neurosecretory system.
Terasawa
Ei
E
Wisconsin National Primate Research Center, Department of Pediatrics, University of Wisconsin, 1223 Capitol Court, Madison, Wisconsin 53715-1299, USA. terasawa@primate.wisc.edu
Keen
Kim L
KL
Grendell
Richard L
RL
Golos
Thaddeus G
TG
D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects; D020013:Calcium Signaling / Q000187:drug effects / Q000235:genetics; D002450:Cell Communication; D002478:Cells, Cultured; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D008253:Macaca mulatta; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D058476:Receptors, Purinergic P2X2; D058848:Receptors, Purinergic P2X4; D014158:Transcription, Genetic
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; D058848:Receptors, Purinergic P2X4; D007987:Gonadotropin-Releasing Hormone; D000255:Adenosine Triphosphate; D002118:Calcium