Three-dimensional co-culture models to study prostate cancer growth, progression, and metastasis to bone.
Cancer-stromal interaction results in the co-evolution of both the cancer cells and the surrounding host stromal cells. As a consequence of this interaction, cancer cells acquire increased malignant potential and stromal cells become more inductive. In this review we suggest that cancer-stromal interaction can best be investigated by three-dimensional (3D) co-culture models with the results validated by clinical specimens. We showed that 3D culture promoted bone formation in vitro, and explored for the first time, with the help of the astronauts of the Space Shuttle Columbia, the co-culture of human prostate cancer and bone cells to further understand the interactions between these cells. Continued exploration of cancer growth under 3D conditions will rapidly lead to new discoveries and ultimately to improvements in the treatment of men with hormonal refractory prostate cancer.
Department of Urology, Molecular Urology and Therapeutics Program, Emory University School of Medicine, Atlanta, GA 30322, USA.
Ruoxiang
R
Wang
Jianchun
J
Xu
Lisa
L
Juliette
Agapito
A
Castilleja
John
J
Love
Shian-Ying
SY
Sung
Haiyen E
HE
Zhau
Thomas J
TJ
Goodwin
Leland W K
LW
Chung
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology / Q000556:secondary*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques; D018450:Disease Progression; D004338:Drug Combinations; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008297:Male; D008953:Models, Anatomic; D008962:Models, Theoretical; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D011509:Proteoglycans / Q000494:pharmacology; D017154:Stromal Cells / Q000473:pathology; D013997:Time Factors
D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen