Long-range surface plasmon resonance imaging for bioaffinity sensors.
A novel bioaffinity sensor based on surface plasmon resonance (SPR) imaging measurements of a multiple-layered structure that supports the generation of long-range surface plasmons (LRSPs) at the water-metal interface is reported. LRSPs possess longer surface propagation lengths, higher electric field strengths, and sharper angular resonance curves than conventional surface plasmons. LRSPR imaging is a version of SPR imaging that requires a symmetric dielectric arrangement around the gold thin film. This arrangement is created using an SF10 prism/Cytop/gold/water multilayer film structure where Cytop is an amorphous fluoropolymer with a refractive index very close to that of water. LRSPR imaging experiments are performed at a fixed incident angle and lead to an enhanced response for the detection of surface binding interactions. As an example, the hybridization adsorption of a 16-mer single-stranded DNA (ssDNA) onto a two-component ssDNA array was monitored with LRSPR imaging. The ssDNA array was created using a new fabrication technology appropriate for the LRSPR multilayers.
Wark
Alastair W
AW
Department of Chemistry, University of California-Irvine, Irvine, California 92697, USA.
Lee
Hye Jin
HJ
Corn
Robert M
RM
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004277:DNA, Single-Stranded / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*
D004277:DNA, Single-Stranded