Identification and expression of the amphioxus Cks1 gene.
A full-length Cks1 homologue gene, AmphiCks1, was identified in amphioxus, Branchiostoma belcheri tsingtauense. Sequence characteristics, phylogeny and patterns of expression during embryonic and larval development were established. The protein predicted from AmphiCks1 showed high sequence identity with vertebrate and invertebrate homologues. Protein structural studies and phylogenetic analysis suggested that Cks homologues are evolutionarily conserved. The AmphiCks1 transcript was detected in most early developmental stages by northern blotting and whole-mount in situ hybridization, suggesting a role for the gene in cell division.
Yang
Hui
H
School of life science, Shandong University, Jinan 250100, China.
Chen
Zhong-Ke
ZK
Zhang
Xiao-Hui
XH
Huang
Xiang-Wei
XW
Zhang
Pei-Jun
PJ
Zhang
Hong-Wei
HW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002816:Chordata, Nonvertebrate / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D030561:Databases, Nucleic Acid; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D015723:Gene Library; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D012333:RNA, Messenger