Molecularly imprinted thin film self-assembled on piezoelectric quartz crystal surface by the sol-gel process for protein recognition.
A novel method of combining sol-gel and self-assembly technology to prepare a human serum albumin (HSA)-imprinted film on the surface of piezoelectric quartz crystal (PQC) Au-electrode modified with thioglycolic acid was described in this paper. The imprinting process was characterized by using the piezoelectric quartz crystal impedance (PQCI) technique and electrochemical impedance technique. Scanning electron microscope (SEM) was employed to characterize the surface morphology of the resultant imprinted film. The piezoelectric technique and electrochemical impedance technique were also employed to investigate the binding performance of the sol-gel-imprinted film with the template protein. The results showed that the imprinted PQC film can give selective recognition to the template protein. The effects of salts and solvents on the binding capacity of the imprinted film with protein were discussed in detail. Other influencing factors (temperature and pH) have also been investigated. This self-assembly sol-gel imprinting technique was proved to be an alternative method for the preparation of biomacromolecule-imprinted thin film.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Zhaohui
Z
Zhang
Yumei
Y
Long
Lihua
L
Nie
Shouzhuo
S
Yao
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D005240:Feasibility Studies; D006801:Humans; D008567:Membranes, Artificial; D044367:Phase Transition; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D011791:Quartz; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D013499:Surface Properties; D014159:Transducers
D008567:Membranes, Artificial; D012709:Serum Albumin; D011791:Quartz