[Generation of A/Chicken/Shanghai/F/98 (H9N2) avian influenza virus from eight plasmids].
Eight-plasmid system was used for the generation of Avian influenza virus (AIV) strain A/Chicken/Shanghai/F/98 (H9N2) which was isolated in China in 1998. In this plasmid-based expression system, viral cDNA was inserted beteen the RNA polymerase I (pol I) promoter and terminator sequences. The entire pol I transcription unit was flanked by an RNA polymerase II (pol II) promoter and a poly (A) site. Twenty-four hours after the transfection of eight expression plasmid into cos1 cells, the supernatant and cos1 cells transfected were inoculated into the allantoic cavity of 10-day-old specific-pothgen-free (SPF) chicken eggs. The HA titer was determined after passage of the rescued virus in chicken eggs, and as high as that of the parental wild-type virus.
Animal Infectious Disease Laboratory, MOA, Yangzhou University, Yangzhou 225009, China. shihuoying2002@hotmail.com
Huo-ying
HY
Shi
Jian-hong
JH
Lu
Su-juan
SJ
Chen
Lei
L
Sun
Xiu-fan
XF
Liu
D000818:Animals; D019556:COS Cells; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral; D012319:RNA Polymerase II; D012318:RNA Polymerase I