Development of a headspace gas chromatography method to determine residual aliphatic amines in oligonucleotides.
A headspace gas chromatographic method has been developed for analysis of aliphatic amines in oligonucleotides. The linearity was improved by including ammonia in the sample vials. Also, carryover of longer chain primary amines, hexylamine and octylamine, was substantially reduced by cleaning the injection syringe with ammonia and water vapor between sample injections.
Von Wald
Grant
G
Analytical Sciences Laboratory, The Dow Chemical Company, 1897 Building, Midland, MI 48667, USA. vonwaldr@tir.com
Albers
Dave
D
Nicholson
Larry
L
Langhorst
Marsha
M
Bell
Bruce
B
D000588:Amines / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000588:Amines; D009841:Oligonucleotides