Coral red fluorescence protein as genetic modified baculovirus tracer.
Genetic modified baculovirus (GMBV) are among the most promising alternatives to chemical insecticides. One of the deterrents to the GMBV development is the lack of simple and cost-effective methods for monitoring their efficacy and ecology in fields. Here, we demonstrate the DsRed gene from coral can serve as a convenient GMBV tracer. Insect larvae, including Trichoplusia ni, Spodoptera exigua, and Spodoptera litura, infected the GMBV containing the DsRed gene can emit red fluorescence under sun light without any prosthetic apparatus.
Jinn
Tzyy-Rong
TR
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Kao
Suey-Sheng
SS
Tzen
Jason T C
JT
Wu
Tzong-Yuan
TY
D000818:Animals; D037421:Anthozoa; D016367:Baculoviridae / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics*; D008856:Microscopy, Fluorescence; D009036:Moths / Q000821:virology; D018411:Spodoptera / Q000821:virology; D000097573:Red Fluorescent Protein
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein