[Induction of specific immunity against tumor endothelial cells by dendritic cells in vitro].
OBJECTIVE
To study the specific cytotoxicity of cytotoxic T lymphocytes(CTLs) induced by dendritic cells(DCs) loaded with tumor-derived endothelial cells (Td-ECs) antigen.


METHODS
Td-ECs were generated from human umbilical vein endothelial cells (HUVECs) by induction with cultured supernatants of tumor cells. The expression of tumor endothelial marker(TEM) was detected by RT-PCR. Human peripheral blood monocyte-derived DCs were loaded with Td-EC freeze-thawing antigen, and the autologous stimulation capacity of DCs was determined via mixed lymphocytes reaction employing MTS colorimetry. Specific cytolytic activity of CTLs induced by DCs was assessed by lactate dehydrogenase(LDH) assay.


RESULTS
TEM1 and TEM8 were detected only on Td-ECs. DCs loaded with Td-EC antigen stimulated lymphocytes proliferation significantly. CTLs induced by DCs loaded with Td-EC antigen showed higher cytolytic activity against Td-EC than that of the control groups (33% vs 14% at E/T ratio 20, 27% vs 10% at E/T ratio 10).


CONCLUSIONS
DC loaded with Td-EC antigen can effectively induce specific CTLs against Td-ECs in vitro.
Xiang
Bang-de
BD
Department of Hepatobiliary Surgery, The First Affiliated Hospital, SUN Yat-Sen University, Guangzhou 510080, China.
Lu
Ming-de
MD
Tang
Qing
Q
Huang
Jie-fu
JF
D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D009369:Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology
D014408:Biomarkers, Tumor