Evaluation of ELISA for detection of Trichinella antibodies in muscle juice samples of naturally infected pigs.
The performance characteristics of an ELISA test for trichinellosis in pigs applied to muscle juice was assessed using 314 samples collected from pigs located in endemic areas of Croatia. Peptic digestion was used as the reference method. The diagnostic accuracy of the two compared dilutions (1:10 and 1:100) was considered to be high because the area under the curve (AUC) index was 0.922 and 0.920 for each dilution, respectively. In this study the two graph-receiver operating characteristic (TG-ROC) analysis was used as a tool for selecting cut-off points. Sensitivity, specificity, likelihood ratios, efficiency and Youden's index were used as indices of test accuracy. The cut-off values that minimize overall misclassification cost under an assumption of 3% prevalence were calculated. Our results indicate that the ELISA applied to muscle juice is a highly accurate test and can be adapted to process a large number of samples.
Department of Parasitology, Veterinary Faculty, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia. reljab@vef.hr
R
R
Beck
A
A
Gaspar
Z
Z
Mihaljević
A
A
Marinculić
D
D
Stojcević
M
M
Brstilo
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000737:chemistry; D017523:Croatia; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015801:Helminth Proteins / Q000737:chemistry; D018482:Muscle, Skeletal / Q000469:parasitology*; D011237:Predictive Value of Tests; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000469:parasitology*; D014234:Trichinella / Q000302:isolation & purification*; D014235:Trichinellosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella