Differentiation of five strains of infectious bursal disease virus: development of a strain-specific multiplex PCR.
Infectious bursal disease virus (IBDV) is a major cause of disease problems in the poultry industry and vaccination has therefore been applied intensively to control the infection. The classical methods of detection and characterization of IBDV are by the use of immunodiffusion test and histopathology. Since these methods are laborious and have low specificity alternatives are needed. In the present study, we report the development of a strain-specific multiplex RT-PCR technique, which can detect and differentiate between field strains of IBDV and vaccine virus strains including a so-called hot vaccine strain widely used in the European poultry industry. The method, which is highly specific, fast and inexpensive, can be applied in all laboratories with basal PCR capabilities and equipment.
Department of Poultry, Fish and Fur Animals, Danish Institute for Food and Veterinary Research, Hangøvej 2, DK-8200, Arhus N, Denmark.
Mette
M
Kusk
Susanne
S
Kabell
Poul H
PH
Jørgensen
Kurt J
KJ
Handberg
D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology*; D002645:Chickens; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D007243:Infectious bursal disease virus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics
D012367:RNA, Viral; D015678:Viral Structural Proteins