Reflectometric interference spectroscopy combined with MALDI-TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives.
This paper describes the combination of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with label free bio-interaction analysis based on reflectometric interference spectroscopy (RIfS). The potential of this concerted approach is demonstrated by measuring the binding properties of different vancomycin-type glycopeptide antibiotic mixtures. Although RIfS is sensitive and does not require use of a label, it cannot determine which components of a mixture have bound to the surface after incubation. Fortunately, each bound species has a unique mass that can, afterwards, be determined by mass spectrometry. Thus, the screening capability of RIfS is combined with the identification capability of mass spectrometry.
Mehlmann
Martin
M
Institute for Physical and Theoretical Chemistry, University of Tübingen, 72074 Tuebingen, Germany.
Garvin
Alex M
AM
Steinwand
Michael
M
Gauglitz
Günter
G
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques; D010316:Particle Size; D010455:Peptides / Q000378:metabolism; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D010455:Peptides; D014640:Vancomycin