Radical scavenging and anti-lipoperoxidative activities of Smallanthus sonchifolius leaf extracts.
Radical scavenging and anti-lipoperoxidative effects of two organic fractions and two aqueous extracts from the leaves of a neglected Andean crop-yacon (Smallanthus sonchifolius Poepp. & Endl., Asteraceae) were determined using various in vitro models. The extracts' total phenolic content was 10.7-24.6%. They exhibited DPPH (IC50 16.14-33.39 microg/mL) and HO* scavenging activities (4.49-6.51 mg/mL). The extracts did not scavenge phenylglyoxylic ketyl radicals, but they retarded their formation. In the xanthine/xanthine oxidase superoxide radical generating system, the extracts' activities were 26.10-37.67 superoxide dismutase equivalents/mg. As one of the extracts displayed xanthine oxidase inhibitory activity, the effect of the extracts on a nonenzymatically generated superoxide was determined (IC50 7.36-21.01 microg/mL). The extracts inhibited t-butyl hydroperoxide-induced lipoperoxidation of microsomal and mitochondrial membranes (IC50 22.15-465.3 microg/mL). These results make yacon leaves a good candidate for use as a food supplement in the prevention of chronic diseases involving oxidative stress.
Palacký University, Faculty of Medicine, Institute of Medical Chemistry and Biochemistry, Hnevotínská 3, CZ-77515 Olomouc, Czech Republic. kata.valentova@email.cz
Katerina
K
Valentová
Frantisek
F
Sersen
Jitka
J
Ulrichová
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000737:chemistry; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D020122:tert-Butylhydroperoxide; C004931:1,1-diphenyl-2-picrylhydrazyl