A preliminary study of applying chemical biliary duct embolization to chemical hepatectomy in rats.
The high recurrence of hepatolithiasis, together with the high operative risk of hepatectomy for specially located stones, has not been settled effectively to date. Thus, this study was designed to investigate the feasibility of applying chemical biliary duct embolization (CBDE) to chemical hepatectomy in rats. As revealed in our results, the intrahepatic biliary ducts could be partially or completely occluded by both phenol and absolute ethanol. In addition, the embolization effect was greatly enhanced by further using cyanoacrylate. Also noteworthy is that CBDE resulted in massive death of hepatocytes, which were replaced by proliferated bile ductules and collagen. More importantly, the hepatocytes disappeared completely in the periphery of the embolized lobe where chemical hepatectomy was achieved. As for the comparison of embolic agents, the combination of phenol and cyanoacrylate exhibited even better fibrogenic effects than the combination of ethanol and cyanoacrylate. In conclusion, CBDE might be a promising approach for achieving the effects of chemical hepatectomy. The combination of phenol and cyanoacrylate potentially acted as a more effective agent for biliary duct embolization.
Li
Fu Yu
FY
Department of Hepatobiliary Surgery, West China Hospital of Sichuan University, Chengdu 610041, China. 1fy_74@vip.163.com
Cheng
Jing Qiu
JQ
He
Sheng
S
Li
Ning
N
Zhang
Ming Ming
MM
Dong
Jia Hong
JH
Jiang
Li Sheng
LS
Cheng
Nan Sheng
NS
Xiong
Xian Zhe
XZ
D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D002779:Cholestasis / Q000139:chemically induced; D003487:Cyanoacrylates / Q000494:pharmacology; D004621:Embolization, Therapeutic / Q000379:methods*; D000431:Ethanol / Q000494:pharmacology; D005240:Feasibility Studies; D022781:Hepatocytes / Q000187:drug effects; D020347:Lithiasis / Q000188:drug therapy; D008099:Liver / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000139:chemically induced*; D008107:Liver Diseases / Q000188:drug therapy; D008297:Male; D023421:Models, Animal; D019800:Phenol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012597:Sclerosing Solutions / Q000494:pharmacology*; D014014:Tissue Adhesives / Q000494:pharmacology*
D003487:Cyanoacrylates; D012597:Sclerosing Solutions; D014014:Tissue Adhesives; D019800:Phenol; D000431:Ethanol