Synthesis and triplex-forming ability of 2',4'-BNAs bearing imidazoles as a nucleobase.
To expand the sequence of double-stranded DNA (dsDNA) targets in a triplex formation, 2',4'-BNAs (2'-O,4'-C-methylene bridged nucleic acids) having imidazoles as a nucleobase were synthesized and incorporated into oligonucleotides. Triplex-forming ability of the modified oligonucleotides was evaluated by using melting temperature (Tm) measurements.
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Yoshiyuki
Y
Hari
Satoshi
S
Obika
Hiroyasu
H
Inohara
Masahiro
M
Ikejiri
Daisuke
D
Une
Takeshi
T
Imanishi
D002851:Chromatography, High Pressure Liquid; D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
D007093:Imidazoles; D009696:Nucleic Acids