Clinical use of 16S rRNA gene sequencing to identify Mycoplasma felis and M. gateae associated with feline ulcerative keratitis.
Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less empirical and more organism-specific therapy.
TriHealth Laboratories and Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. larry_gray@trihealth.com
Larry D
LD
Gray
Kerry L
KL
Ketring
Yi-Wei
YW
Tang
D000818:Animals; D015373:Bacterial Typing Techniques; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology*; D002415:Cats; D003315:Cornea / Q000382:microbiology; D003320:Corneal Ulcer / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S