Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences.
The 18S small subunit ribosomal RNA (18S rRNA) gene of an unnamed Babesia species (designated B. U sp.) was sequenced and analyzed in an attempt to distinguish it from other Babesia species in China. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was ligated to the pGEM-T Easy vector for sequencing. It was found that the length of the 18S rRNA gene of all B. U sp. Kashi 1 and B. U sp. Kashi 2 was 1699 bp and 1689 bp. Two phylogenetic trees were, respectively, inferred based on 18S rRNA sequence of the Chinese bovine Babesia isolates and all of Babesia species available in GenBank. The first tree showed that B. U sp. was situated in the branch between B. major Yili and B. bovis Shannxian, and the second tree revealed that B. U sp. was confined to the same group as B. caballi. The percent identity of B. U sp. with other Chinese Babesia species was between 74.2 and 91.8, while the percent identity between two B. U sp. isolates was 99.7. These results demonstrated that this B. U sp. is different from other Babesia species, but that two B. U sp. isolates obtained with nymphal and adultal Hyalomma anatolicum anatolicum tick belong to the same species.
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 11, Lanzhou, Gansu 730046, PR China. ttbdlvri@public.lz.gs.cn
Jianxun
J
Luo
Hong
H
Yin
Zhijie
Z
Liu
Dongying
D
Yang
Guiquan
G
Guan
Aihong
A
Liu
Miling
M
Ma
Shengzhi
S
Dang
Bingyi
B
Lu
Caiqin
C
Sun
Qi
Q
Bai
Wenshun
W
Lu
Puyan
P
Chen
D000818:Animals; D001403:Babesia / Q000235:genetics*; D001404:Babesiosis / Q000469:parasitology / Q000662:veterinary*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002681:China; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S