Modification of beta-lactoglobulin by oligofructose: impact on protein adsorption at the air-water interface.
Maillard products of beta-lactoglobulin (betaLg) and fructose oligosaccharide (FOS) were obtained in different degrees of modification depending on incubation time and pH. By use of a variety of biochemical and spectroscopic tools, it was demonstrated that the modification at limited degrees does not significantly affect the secondary, tertiary, and quaternary structure of betaLg. The consequence of the modification on the thermodynamics of the protein was studied using differential scanning calorimetry, circular dichroism, and by monitoring the fluorescence intensity of protein samples with different concentrations of guanidine-HCl. The modification leads to lowering of the denaturation temperature by 5 degrees C and a reduction of the free energy of stabilization of about 30%. Ellipsometry and drop tensiometry demonstrated that upon adsorption to air-water interfaces in equilibrium modified betaLg exerts a lower surface pressure than native betaLg (16 versus 22 mN/m). Moreover, the surface elastic modulus increased with increasing surface pressure but reached significantly smaller values in the case of FOS-betaLg. Compared to native betaLg, modification of the protein with oligofructose moieties results in higher surface loads and thicker surface layers. The consequences of these altered surface rheological properties are discussed in view of the functional behavior in technological applications.
Wageningen Centre for Food Sciences, Diedenweg 20, 6700 AN Wageningen, The Netherlands.
Daria
D
Trofimova
Harmen H J
HH
de Jongh
D000327:Adsorption; D000388:Air; D002852:Chromatography, Ion Exchange; D004548:Elasticity; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D044367:Phase Transition; D017510:Protein Folding; D017433:Protein Structure, Secondary; D013057:Spectrum Analysis; D013816:Thermodynamics; D013997:Time Factors; D014783:Viscosity; D014867:Water / Q000737:chemistry*
D007782:Lactoglobulins; D009844:Oligosaccharides; C120489:oligofructose; D014867:Water