Comparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle.
A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot-ELISA, was more convenient in field application. By the test (dot-ELISA) the infection could be equally detected in animals infected with 100, 200 and 300 metacercariae of F. gigantica with high sensitivity. Further, the antigen (27 kDa) was not found to react with goat sera infected with Paramphistomum epiclitum, which are giving strong reaction to homologous immature and mature fluke antigens of P. epiclitum.
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India. sghoshp@yahoo.co.in
S
S
Ghosh
Preeti
P
Rawat
S C
SC
Gupta
B P
BP
Singh
D000818:Animals; D000909:Antibodies, Helminth / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D000947:Antigens, Helminth / Q000737:chemistry; D002417:Cattle; D003488:Cyanogen Bromide / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005209:Fasciola / Q000378:metabolism*; D005211:Fascioliasis / Q000175:diagnosis*; D006023:Glycoproteins / Q000737:chemistry; D006041:Goats; D007074:Immunoglobulin G / Q000737:chemistry; D008195:Lymnaea; D011817:Rabbits; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000737:chemistry; D013997:Time Factors; D014201:Trematode Infections / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000941:Antigens; D000947:Antigens, Helminth; D006023:Glycoproteins; D007074:Immunoglobulin G; D012685:Sepharose; D003488:Cyanogen Bromide