Enzyme-cleavable linkers for peptide and glycopeptide synthesis.
Hydroxymethylphenoxy linkers that are commonly used in solid phase peptide synthesis are surprisingly susceptible to efficient cleavage by the protease chymotrypsin with a broad range of amino acid residues being tolerated at the scissile bond; this enzyme-cleavable linker system has been applied to peptide and glycopeptide synthesis.
School of Chemistry, Edinburgh University, Kings Buildings, West Mains Road, Edinburgh, UK EH9 3JJ.
Beatrice A
BA
Maltman
Mallesham
M
Bejugam
Sabine L
SL
Flitsch
D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D006868:Hydrolysis; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D003432:Cross-Linking Reagents; D006020:Glycopeptides; D010455:Peptides; D002918:Chymotrypsin