Expression of beta-defensin-2 in human gastric tumors: a pilot study.
The present work was AIMED on the study of patterns of human beta-defensin-2 (hBD-2) expression in human gastric tumors.


METHODS
17 samples of surgically resected human gastric adenocarcinoma paired with respective normal tissue controls have been analyzed for the expression of hBD-2 by semi-quantitative RT-PCR and by immunohistochemical analysis using anti-hBD-2 monoclonal antibodies (mAbs) generated against recombinant hBD-2 by standard hybridoma technology.


RESULTS
The hyperexpression of hBD-2 on mRNA and protein levels was registered in 6 from 17 gastric tumor samples compared to respective controls; however, hBD-2 expression patterns seems not be related to the differentiation grade and stage of tumors and the presence of anti-Helicobacter pylori-antibodies in the blood serum of the patients.


CONCLUSIONS
In human gastric adenocarcinomas expression of hBDs possesses heterogeneic character; the research on larger cohort of the patients with gastric cancer should be further performed.
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine. pogrebnoy@onconet.kiev.ua
Natalya
N
Markeeva
Igor
I
Lisovskiy
Valeriy
V
Lyzogubov
Vasilij
V
Usenko
Maria
M
Soldatkina
Serhey
S
Merentsev
Serhey
S
Zaitsev
Yuriy
Y
Kondratskii
Anatoliy
A
Tofan
Serhey
S
Osinskiy
Petro
P
Pogrebnoy
D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D005753:Gastric Mucosa / Q000378:metabolism; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009367:Neoplasm Staging; D010865:Pilot Projects; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D023083:beta-Defensins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
C106641:DEFB4A protein, human; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D023083:beta-Defensins