Effects of Jiantaiye on estrogen receptor and mRNA expressions in uterus of mice with embryo implantation dysfunction.
OBJECTIVE
To explore the effect of Jiantaiye (JTY) on the expression of estrogen receptor (ER) and ER mRNA in uterus of mice with embryo implantation dysfunction.


METHODS
Embryo implantation dysfunction mouse models were induced with mifepristone and treated with JTY. All animals were sacrificed on day 8 of pregnancy. The endometrial ER and ER mRNA expressions were assessed by immunnohistochemical SP method and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
Area ratio and absorbency of ER in the JTY treated group's gland and stroma were higher than those of the model group, quite similar to those of the normal control's, and ER mRNA expression in treated group's uterus was significantly higher than that in the models, but it was not significantly different from the normal control.


CONCLUSIONS
JTY improves the endometrial development by increasing ER and ER mRNA expressions of uterus of mice with embryo implantation dysfunction.
Liu
Yan-juan
YJ
Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. tjyjliu@hotmail.com
Huang
Guang-ying
GY
Yang
Ming-wei
MW
Gong
Ping
P
Zhang
Ming-min
MM
Lu
Fu-er
FE
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010064:Embryo Implantation; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D013194:Staining and Labeling; D014018:Tissue Distribution; D014591:Uterine Diseases / Q000276:immunology / Q000503:physiopathology*; D014599:Uterus / Q000187:drug effects* / Q000276:immunology*
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D011960:Receptors, Estrogen