[Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP.].
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
Hu
Xiao-Fen
XF
Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China, Email: hxf999.student@sina.com.
Xie
Bei
B
Yu
Rui-Song
RS
Huang
Qi-Zhong
QZ
Zhang
De-Fu
DF
Huang
Lu-Sheng
LS
Li
Zhen
Z
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics; D002678:Chimera; D047108:Embryonic Development; D005854:Germ Cells