Genetic markers on the HTLV-1 p12I protein sequences from Brazilian HAM/TSP patients and asymptomatic HTLV-1 carrier isolates.
The human T cell leukemia/lymphotropic virus type-1 (HTLV-1) genome has approximately 9 kb and contains the pX region that codes for regulatory and accessory proteins. The pX ORF-I encodes for the p12 protein, a 99 aa peptide, which presents several functional putative domains, such as leucine zipper motifs, SH3- binding motifs, and a dileucine motif, p12I. Also, a rare p12IK88 allele was found mainly in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients, suggesting it is a marker of pathogenesis, although recent studies showed p12IK in asymptomatic HTLV-1 carriers. To extend the observations on p12I motifs, we sequenced 26 p12I from HAM/TSP patients and asymptomatic HTLV-1 carriers. Amino acid analysis of 48 p12I motifs demonstrated the presence of several alleles, but the allelic variation, including p12IK, was not prevalent in HAM/TSP isolates. Nonetheless, some genetic markers were recognized in association with isolates from HTLV-1a subgroup B and Brazilian HTLV-1aA strains.
Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, 21045-900, Brazil. alena@ioc.fiocruz.br
Alena M
AM
Iñiguez
Koko
K
Otsuki
Gustavo P
GP
Magalhães
Edimilson A
EA
Silva
Ana Carolina P
AC
Vicente
D000595:Amino Acid Sequence; D001938:Brazil; D002353:Carrier State / Q000821:virology*; D005819:Genetic Markers; D015490:HTLV-I Infections / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000235:genetics; D015493:Paraparesis, Tropical Spastic / Q000821:virology*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
D005819:Genetic Markers; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins; C084305:p12I protein, Human T-lymphotropic virus 1