First isolation of Streptococcus downei from human dental plaques.
In this study, we isolated four bacterial strains grown on mitis-salivarius sucrose bacitracin agar. The strains had similar biochemical characteristics to biotypes I or II of mutans streptococci. The four isolates were identified as Streptococcus downei by 16S rDNA and dextranase gene (dex) sequencing as well as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) targeting dex. To our knowledge, this is the first report of the isolation and identification of S. downei from dental plaque in humans. The results suggest that S. downei can inhabit the human oral cavity.
Yoo
So Young
SY
Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea.
Kim
Kwan-Joong
KJ
Lim
Seong-Hoon
SH
Kim
Kwang-Won
KW
Hwang
Ho-Keel
HK
Min
Byung-Moo
BM
Choe
Son-Jin
SJ
Kook
Joong-Ki
JK
D003470:Culture Media; D004275:DNA, Ribosomal / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013291:Streptococcus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D013395:Sucrose
D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013395:Sucrose