Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.
Carter
Mark G
MG
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA. cartermar@mail.nih.gov
Sharov
Alexei A
AA
VanBuren
Vincent
V
Dudekula
Dawood B
DB
Carmack
Condie E
CE
Nelson
Charlie
C
Ko
Minoru S H
MS
D000818:Animals; D001483:Base Sequence; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D016678:Genome; D007438:Introns; D051379:Mice; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger