Effect of adrenomedullin administration on acetic acid-induced colitis in rats.
Adrenomedullin (AM) administered intracolonically ameliorated the severity of acetic acid-induced colonic ulceration in rats. Ulcers were induced by subserosal injection of acetic acid into the colon. AM-treated group was administered 0.25-1.0 microg of AM in 0.5 ml of saline intracolonically once a day; the control group received only saline. AM administration dose-dependently and significantly reduced the size of the ulcerative lesions, the associated edema, and the infiltration of the affected area by inflammatory cells. AM also reduced tissue levels of interleukin-6, but not interferon-gamma. AM reduces the severity of acetic acid-induced colitis in rats, probably by inhibiting the production and/or release of Th-2 cell-derived factors such as interleukin-6.
First Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. sashi@fc.med.miyazaki-u.ac.jp
Shinya
S
Ashizuka
Naoto
N
Ishikawa
Johji
J
Kato
Junichi
J
Yamaga
Haruhiko
H
Inatsu
Tanenao
T
Eto
Kazuo
K
Kitamura
D019342:Acetic Acid / Q000633:toxicity*; D053607:Adrenomedullin; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D007202:Indicators and Reagents / Q000633:toxicity*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D007371:Interferon-gamma; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D010455:Peptides / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D018418:Th2 Cells / Q000378:metabolism / Q000473:pathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D015850:Interleukin-6; D010455:Peptides; D053607:Adrenomedullin; D007371:Interferon-gamma; D019342:Acetic Acid