Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections.
A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.
Arctic Investigations Program, CDC, 4055 Tudor Centre Drive, Anchorage, AK 99508, USA. jmorris@cdc.gov
Julie M
JM
Morris
Alisa L
AL
Reasonover
Michael G
MG
Bruce
Dana L
DL
Bruden
Brian J
BJ
McMahon
Frank D
FD
Sacco
Douglas E
DE
Berg
Alan J
AJ
Parkinson
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D005456:Fluorescent Dyes; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008826:Microbial Sensitivity Tests; D016612:Paraffin Embedding; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013270:Stomach; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; D017291:Clarithromycin