Near IR emitting isothiocyanato-substituted fluorophores: their synthesis and bioconjugation to monoclonal antibodies.
Two near IR emitting fluorophores, based on the phthalocyanine and naphthalocyanine chromophores, which also bear a single isothiocyanato group suitable for conjugation to proteins are reported; their utility as luminescent probes is demonstrated by conjugation to monoclonal antibodies and the ability of these conjugates to selectively bind cells bearing the relevant antigen.
Duan
Wubiao
W
University of Hull, Department of Chemistry and Clinical Biosciences Institute, Hull, UKHU6 7RX.
Smith
Karen
K
Savoie
Huguette
H
Greenman
John
J
Boyle
Ross W
RW
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D007211:Indoles / Q000737:chemistry; D054833:Isoindoles; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D017879:Isothiocyanates; C013647:phthalocyanine