Identification and characterization of a novel family of Drosophila beta-adrenergic-like octopamine G-protein coupled receptors.
Insect octopamine receptors carry out many functional roles traditionally associated with vertebrate adrenergic receptors. These include control of carbohydrate metabolism, modulation of muscular tension, modulation of sensory inputs and modulation of memory and learning. The activation of octopamine receptors mediating many of these actions leads to increases in the levels of cyclic AMP. However, to date none of the insect octopamine receptors that have been cloned have been convincingly shown to be capable of directly mediating selective and significant increases in cyclic AMP levels. Here we report on the identification and characterization of a novel, neuronally expressed family of three Drosophila G-protein coupled receptors that are selectively coupled to increases in intracellular cyclic AMP levels by octopamine. This group of receptors, DmOct beta1R (CG6919), DmOct beta2R (CG6989) and DmOct beta3R (CG7078) shows homology to vertebrate beta-adrenergic receptors. When expressed in Chinese hamster ovary cells all three receptors show a strong preference for octopamine over tyramine for the accumulation of cyclic AMP but show unique pharmacological profiles when tested with a range of synthetic agonists and antagonists. Thus, the pharmacological profile of individual insect tissue responses to octopamine might vary with the combination and the degree of expression of the individual octopamine receptors present.
The Inositide Laboratory, The Babraham Institute, Cambridge, UK.
Braudel
B
Maqueira
Heather
H
Chatwin
Peter D
PD
Evans
D018663:Adrenergic Agents / Q000494:pharmacology; D020816:Amino Acid Motifs / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D016466:CHO Cells / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D009655:Octopamine / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000379:methods; D014162:Transfection / Q000379:methods
D018663:Adrenergic Agents; D029721:Drosophila Proteins; C503410:Octbeta1R protein, Drosophila; C503411:Octbeta2R protein, Drosophila; C503412:Octbeta3R protein, Drosophila; D011943:Receptors, Adrenergic, beta; D043562:Receptors, G-Protein-Coupled; D009655:Octopamine; D005576:Colforsin; D000242:Cyclic AMP; D002118:Calcium