Phospholipid solubility determined by equilibrium distribution between surface and bulk phases.
A general strategy is proposed for determining the very low aqueous solubility limits of bilayer-forming phospholipids. The strategy exploits the inherent surface activity of phospholipids and has been termed EDSB, which stands for Equilibrium Distribution between Surface and Bulk phases. In this report, EDSB has been used to determine the critical bilayer concentration of dilauroylphosphatidylycholine (DLPC), a short-chain bilayer-forming phospholipid. At room temperature in neutral pH buffer, CBC(DLPC) = 2.5 x 10(-)(8) M. Using a mole fraction concentration scale, this corresponds to a standard-state free energy change of -12.8 kcal/mol for DLPC bilayer membrane formation.
Buboltz
Jeffrey T
JT
Field of Biophysics, Cornell University, Ithaca, New York 14853, USA. jbuboltz@colgate.edu
Feigenson
Gerald W
GW
D002021:Buffers; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D010713:Phosphatidylcholines / Q000737:chemistry*; D012588:Scintillation Counting; D012995:Solubility; D014867:Water
D002021:Buffers; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014867:Water; C015153:1,2-dilauroylphosphatidylcholine