Protein dynamics of bovine dentin phosphophoryn.
Bovine dentin phosphophoryn (BDP), a protein rich in aspartyl (Asp) and o-phosphoseryl [Ser(P)] residues, is synthesized by odontoblasts and believed to be involved in matrix-mediated biomineralization of dentin. The elucidation of the structure-function relationship of phosphophoryn has been a challenge because of its high-molecular weight, high negative charge, repetitive sequence, and lability. We have used the dynamic behavior of the (1)H NMR signal at 600 MHz to provide insight into the molecular dynamics of phosphophoryn. Our results indicate that phosphophoryn is a molecule of uniformly high mobility, thus belonging to a recently identified class of intrinsically disordered proteins that are characterized by sequences of low complexity and rich in polar and charged residues. The significance of our results is that phosphophoryn, because of its uniform nature has the potential to be replaced by biomimetic synthetic peptide analogs that together with amorphous calcium phosphate may lead to the development of novel, nontoxic, apatite-based dental restorative materials.
Cross
K J
KJ
Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne, Melbourne, Australia.
Huq
N L
NL
Reynolds
E C
EC
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003804:Dentin / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009804:Odontoblasts; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013329:Structure-Activity Relationship
D010750:Phosphoproteins; C017630:phosphophoryn; D002118:Calcium