[Changes of ultrastructure and p-53 protein expression on the peripheral blood lymphocytes of patients with vulgar form of psoriasis after balneal therapy in resort "nunisi"].
Analysis of peripheral blood lymphocytes ultrastructure and P-53 apoptotic protein expression in patients with psoriasis before and after balneal therapy in "Nunisi" has been performed. Samples of peripheral blood of 10 patients (22-46 years of age) with vulgar form of psoriasis (4--disseminated form, 6--focal) were investigated. Duration of illness--from 2 to 24 years, remission--6-12 months. Balneal bath therapy in "Nunisi" included the course of 15-18 procedures, t degrees--36-37 degrees. Total number of patients under our observation was 65, 25 of them received basic therapy, 40--balneal therapy. 25 persons were practically healthy, 5 of them were blood donors. Cells of lymphoid population from peripheral blood were investigated by electron microscopy and immunohistochemistry using of P-53 protein's marker ("Novo Castra") visualized by system "DAB". Character of P-53 preapoptotic protein expression and changes in lymphocytes ultrastructure testify to the trend of lymphocytes necrosis and apoptosis development especially in large granular lymphocytes during basic therapy. After the course of balneal therapy in "Nunisi", large granular lymphocytes, with signs of protein synthetic activity, as well as the psoriatic lesion foci in epidermis were reduced.
A.Natishvili Institute of Experimental Morphology, Academy of Sciences of Georgia.
L
L
Rukhadze
Z
Z
Tsagareli
L
L
Gogiashvili
L
L
Nebolsina
R
R
Gudjabidze
D000328:Adult; D001494:Baths; D005260:Female; D006294:Health Resorts; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53