Phytoestrogen-rich herb formula "XLGB" prevents OVX-induced deterioration of musculoskeletal tissues at the hip in old rats.
This study investigated a phytoestrogen-rich herb formula, Xianlinggubao (XLGB) (including genistein 510 microg/g and daidzein 2500 microg/g), concerning prevention of OVX-induced deterioration of musculoskeletal tissues in 11-month-old female Wistar rats, which were randomized into Sham, OVX, and XLGB groups. Daily oral administration of XLGB (250 mg/kg/day) started after OVX for 3 months. mRNA of MHC-I IIa IIb of abductor muscle was determined by RT-PCR. The proximal femoral BMD and geometry, microarchitecture, and mechanical strength were evaluated by pQCT, micro-CT, and compressive testing, respectively. The bone turnover biochemical markers serum osteocalcin (OC) and urinary deoxypyridinoline (DPD) were evaluated. The results showed that (1) XLGB-treated OVX rats showed no difference compared to the Sham group, whereas OVX induced significant deterioration in variables related to bone density, microarchitecture, and mechanical strength (P < 0.05); (2) biochemical markers showed no difference between sham and XLGB groups as compared with higher bone turnover in OVX rats (P < 0.05); (3) mRNA expression of MHC-I IIa IIb was downregulated in OVX rats but upregulated after XLGB treatment (P < 0.05); and (4) as compared with the OVX group, no uterine hypertrophy was found in XLGB-treated rats. In conclusion, findings of this study suggested that the herbal preparation XLGB was able to prevent OVX-induced deterioration of musculoskeletal tissues at the hip without causing uterine stimulation.
Qin
Ling
L
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong SAR, PR China. lingqin@cuhk.edu.hk
Zhang
Ge
G
Hung
Wing-Yin
WY
Shi
Yinyu
Y
Leung
Kwoksui
K
Yeung
Hiu-Yan
HY
Leung
Pingchung
P
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015519:Bone Density / Q000187:drug effects; D005260:Female; D005269:Femur / Q000378:metabolism / Q000473:pathology; D019833:Genistein / Q000008:administration & dosage*; D006131:Growth Inhibitors / Q000008:administration & dosage*; D007529:Isoflavones / Q000008:administration & dosage*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D010024:Osteoporosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D010052:Ovariectomy; D048789:Phytoestrogens / Q000008:administration & dosage*; D028321:Plant Preparations / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D006131:Growth Inhibitors; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; C004742:daidzein; D019833:Genistein