Retroviral expression screening of oncogenes in natural killer cell leukemia.
Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens (n=8).
Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan.
Young Lim
YL
Choi
Ryozo
R
Moriuchi
Mitsujiro
M
Osawa
Atsushi
A
Iwama
Hideki
H
Makishima
Tomoaki
T
Wada
Hiroyuki
H
Kisanuki
Ruri
R
Kaneda
Jun
J
Ota
Koji
K
Koinuma
Madoka
M
Ishikawa
Shuji
S
Takada
Yoshihiro
Y
Yamashita
Kazuo
K
Oshimi
Hiroyuki
H
Mano
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009154:Mutation; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D012190:Retroviridae / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D018631:ras Proteins
D018076:DNA, Complementary; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins