Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects.
In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse model. The molecular weight and antigenic property of P23 expressed by Tg/P23 were similar to those of the native P23. Mice immunized with lysed Tg/P23 tachyzoites produced specific neutralizing antibodies against C. parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals.
National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokaido, Japan.
Hiroaki
H
Shirafuji
Xuenan
X
Xuan
Isao
I
Kimata
Yasuhiro
Y
Takashima
Shinya
S
Fukumoto
Haruki
H
Otsuka
Hideyuki
H
Nagasawa
Hiroshi
H
Suzuki
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D015153:Blotting, Western; D002417:Cattle; D003457:Cryptosporidiosis / Q000517:prevention & control*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D016056:Immunodominant Epitopes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic