Direct interaction between metastasis-associated protein 1 and endophilin 3.
The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3.
Institute for Clinical Research, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka 811-1395, Japan.
Yuko
Y
Aramaki
Katsuhiro
K
Ogawa
Yasushi
Y
Toh
Takahiro
T
Ito
Nobuyoshi
N
Akimitsu
Hiroshi
H
Hamamoto
Kazuhisa
K
Sekimizu
Kimihiko
K
Matsusue
Akira
A
Kono
Haruo
H
Iguchi
Soichi
S
Takiguchi
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D018797:Cell Cycle Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012097:Repressor Proteins; D013737:Testis / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution; D015534:Trans-Activators; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C503336:Mta1 protein, mouse; D012333:RNA, Messenger; D012097:Repressor Proteins; C486100:Sh3glb1 protein, mouse; C435983:Spag5 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors