Genomic analysis of heat-shock factor targets in Drosophila.
We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK. ib107@cam.ac.uk
Ian
I
Birch-Machin
Shan
S
Gao
David
D
Huen
Richard
R
McGirr
Robert A H
RA
White
Steven
S
Russell
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D023281:Genomics; D006360:Heat-Shock Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014158:Transcription, Genetic
D017931:DNA Primers; D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins