Synthesis and DNA-binding affinities of monomodified berberines.
Four new monomodified berberines have been synthesized in moderate to good yields starting from berberine and fully characterized by HRMS and 1H NMR. Spectrometric titration and ethidium bromide displacement experiments indicate that these berberine derivatives, especially the one having primary amino group, strongly bind with calf-thymus DNA, presumably via an intercalation mechanism.
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
Ji-Yan
JY
Pang
Yong
Y
Qin
Wen-Hua
WH
Chen
Guo-An
GA
Luo
Zhi-Hong
ZH
Jiang
D001599:Berberine / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure
D001599:Berberine; D004247:DNA