[An experimental study on proliferative effects and apoptotic effects of podophyllic acid in K562 cells].
OBJECTIVE
To study the effects of podophyllic acid on proliferation and apoptosis in human leukemia K562 cells.


METHODS
The inhibitory effects of podophyllic acid on K562 cells were investigated by clony-formation and MTT colorimetric assay. The apoptotic effects were observed by FCM.


RESULTS
Podophylic acid could inhibit the proliferation of K562 cells and there were a significant difference between experimental and control groups (P < 0.01). There was a marked positive correlation between drug concentration and the inhibitory rates. Podophyllic acid could induce apoptosis of K562 cells. When K562 cells were treated with 5.0 microg/ml podophyllic acid for 48 hours, apoptosis occurred to the largest extent. The apoptosis rate of podophyllic acid was 33.5%.


CONCLUSIONS
Podophyllic acid has antitumor effect, but the change of structure affects the function of derivatives of podophylloxin greatly.
The Affiliated First Hospital of Lanzhou University, Lanzhou 730000.
Jian
J
Yu
Che
C
Chen
Peilong
P
Gao
Zhengping
Z
Jia
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D010946:Plants, Medicinal / Q000737:chemistry*; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011035:Podophyllum / Q000737:chemistry*; D013997:Time Factors
D000972:Antineoplastic Agents, Phytogenic; C507546:podophyllic acid; D011034:Podophyllotoxin