Biological functions of biotinylated histones.
Histones H1, H2A, H2B, H3 and H4 are DNA-binding proteins that mediate the folding of DNA into chromatin. Various posttranslational modifications of histones regulate processes such as transcription, replication and repair of DNA. Recently, a novel posttranslational modification has been identified: covalent binding of the vitamin biotin to lysine residues in histones, mediated by biotinidase and holocarboxylase synthetase. Here we describe a novel peptide-based technique, which was used to identify eight distinct biotinylation sites in histones H2A, H3 and H4. Biotinylation site-specific antibodies were generated to investigate biological functions of histone biotinylation. Evidence was provided that biotinylation of histones plays a role in cell proliferation, gene silencing and cellular response to DNA damage.
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA.
Nagarama
N
Kothapalli
Gabriela
G
Camporeale
Alice
A
Kueh
Yap C
YC
Chew
Anna M
AM
Oommen
Jacob B
JB
Griffin
Janos
J
Zempleni
D000818:Animals; D001710:Biotin / Q000378:metabolism; D019921:Biotinylation; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans
D002843:Chromatin; D004798:Enzymes; D006657:Histones; D001710:Biotin