[Studies of Calotes versicolor on the sexual function amelioration mechanism by hypothalamus inhibition in immature male rats].
OBJECTIVE
To study the amelioration mechanism of Calotes versicolor petroleum ether extracts (CVPEE) on hypothalamus inhibition sexual function induced by morphine in immature male rats.


METHODS
The hypothalamus inhibition induced by morphine in immature male rats was used as model of the testis reproductive functional disturbance. The rats was given CVPEE for 21 days. The levels of testosterone (T) and follicle stimulating hormone (FSH) in serum were determined by the radio-immunoassay. The nitrogen monoxide (NO) contents in serum and penis tissue were determined by nitrate reductase method. The sexual organ and auxiliary sexual organ coefficient were calculated and the histopathological changes in the testes and epididymides were observed with microscope.


RESULTS
CVPEE groups (low and high dosage groups) significantly enhanced the level of serum T, compared with model group (P < 0.05); the low dosage group decreased obviously the level of FSH. CVPEE had the tendency to increase NO contents in serum and penile tissue. The microscopic examination results showed that the testis tissue in CVPEE group was damaged more gently than those in model group.


CONCLUSIONS
CVPEE can enhance the level of serum T and NO contents in penile tissue, improve the sexual functional disturbance in hypothalamus inhibition induced by morphine in immature male rats. It indicated that CVPEE had the effects by the hypothalamus-hypophysis-gonad axial function.
Guangxi College of TCM, Nanning 530001.
Xuemei
X
Liu
Jinxian
J
Xie
Aiyuan
A
Li
Fang
F
Zhou
D000728:Androgens / Q000097:blood; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000097:blood; D007031:Hypothalamus / Q000187:drug effects* / Q000473:pathology; D008116:Lizards; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D009020:Morphine; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012735:Sexual Dysfunction, Physiological / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000097:blood
D000728:Androgens; D008419:Materia Medica; D009569:Nitric Oxide; D013739:Testosterone; D009020:Morphine; D005640:Follicle Stimulating Hormone