Biological performances of collagen-based scaffolds for vascular tissue engineering.
Collagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular tissue engineering. In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent haemocompatibility and cell viability performance assays. First, haemoglobin-free, thrombelastography and platelet adhesion tests were performed in order to investigate the blood contact performance. Secondly, specimens were seeded with endothelial cells and smooth muscle cells, and cell viability tests were carried out by MTT and SEM. Results show that neutralized acid-soluble type I collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation. Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells.
Laboratory for Biomaterials and Bioengineering, Laval University, Québec City, G1K 7P4, Canada.
F
F
Boccafoschi
J
J
Habermehl
S
S
Vesentini
D
D
Mantovani
D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology / Q000254:growth & development*; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008297:Male; D008422:Materials Testing; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D010973:Platelet Adhesiveness / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D024042:Collagen Type I