BLM helicase complements disrupted type II telomere lengthening in telomerase-negative sgs1 yeast.
Recombination-mediated pathways for telomere lengthening may be utilized in the absence of telomerase activity. The RecQ-like helicases, BLM and Sgs1, are implicated in recombination-mediated telomere lengthening in human cells and budding yeast, respectively. Here, we show that BLM expression rescues disrupted telomere lengthening in telomerase-negative sgs1 yeast. BLM helicase activity is required for this complementation, indicating BLM and Sgs1 resolve the same telomeric structures. These data support a conserved function for BLM and Sgs1 in recombination-mediated telomere lengthening.
Lillard-Wetherell
Kate
K
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524, USA.
Combs
Kelly A
KA
Groden
Joanna
J
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004265:DNA Helicases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D053484:RecQ Helicases; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019098:Telomerase / Q000172:deficiency*; D016615:Telomere / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D029701:Saccharomyces cerevisiae Proteins; D019098:Telomerase; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; C090472:SGS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases