[Study of Five STR Loci in A Chinese Han Population.].
To understanding the allele structure and genetic polymorphisms at five STR loci in Chinese Han population, and construct a preliminary database, EDTA-blood specimens were collected from unrelated individuals. DNA samples were extracted with Chelex method and were amplified by PCR technique. The PCR products were analyzed using both PAGE horizontal electrophoresis with discontinuous buffer system and automated fluorescence detection approach. As a result, three STRs consist of simple repeat motifs, while two STR contain a complex repeat structure. The STR polymorphisms at all of the five loci have been observed in Chinese Han population. In a word, the obtained data are beneficial to understanding the population genetics of the five STR loci in Chinese Han population. As a simple approach, the PAGE horizontal electrophoresis can be employed for typing the five STR markers.
School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhen-Jun
ZJ
Jia
Jin
J
Wu
Hui
H
Li
Yiping
Y
Hou
Wei-Juan
WJ
Zhang
Xue-Ping
XP
Zhou
Jian-Qiang
JQ
Deng
Mei-Lin
ML
Shi
Ji
J
Zhang
Ying-Bi
YB
Li
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D005828:Genetics, Population; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences