The effects of flash-annealing on glycerol kinase crystals.
Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (eta) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.
Brown University, Department of Molecular Biology, Cell Biology and Biochemistry, 70 Ship Street, Providence, RI 02903, USA.
Ardeschir
A
Vahedi-Faridi
Vivian
V
Stojanoff
Joanne I
JI
Yeh
D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D016983:Enterococcus / Q000201:enzymology; D005615:Freezing; D005991:Glycerol Kinase / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D005991:Glycerol Kinase