[Breeding of high-yield strain of taxol by mutagensis of protoplast and primary discussion of genetic differences between mutants and their parent strain].
The breeding of high-yield strain of taxol was performed by protoplast mutagenesis of strain NCEU-1 using ultraviolet radiation and combined treatment of UV and LiCl. The mutants UV40-19 and UL50-6 were obtained, which raised the taxol yield from 314.07 microg/L to 376.38 microg/L and 392.63 microg/L respectively. Genetic differences between the mutants UV40-19, UL50-6 and their parent strain were primarily compared through random amplified polymorphic DNA (RAPD) and isozyme technique. The results showed that the genetic differences were very obviously between the parent strain and its mutants and between the two mutants, which laid foundation of molecular mechanism for the study of genes related to the taxol biosynthesis and mutants for raising the taxol yield.
Life and Science college of Hei Longjiang University, Harbin 150080, China. zk395@yahoo.com.cn
Kai
K
Zhao
Wen-xiang
WX
Ping
Xi
X
Ma
Jun
J
Liu
Dong-po
DP
Zhou
D001203:Ascomycota / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D014644:Genetic Variation; D018021:Lithium Chloride; D016296:Mutagenesis; D017239:Paclitaxel / Q000096:biosynthesis*; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D019105:Random Amplified Polymorphic DNA Technique; D014466:Ultraviolet Rays
D018021:Lithium Chloride; D017239:Paclitaxel