A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection.
A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30 degrees C. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50 x 10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.
Ege University Science Faculty, Chemistry Department, Bornova, Izmir, Turkey. niler@mail.ege.edu.tr
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Oztürk
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Ertaş
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Akyilmaz
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Dinçkaya
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Tural
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D002474:Cell-Free System / Q000737:chemistry*; D031943:Cynara scolymus / Q000737:chemistry*; D005780:Gelatin; D006861:Hydrogen Peroxide / Q000032:analysis*; D008567:Membranes, Artificial; D008892:Milk / Q000737:chemistry; D011138:Polytetrafluoroethylene
D008567:Membranes, Artificial; D005780:Gelatin; D011138:Polytetrafluoroethylene; D006861:Hydrogen Peroxide