HPLC determination of telmisartan in human plasma and its application to a pharmacokinetic study.
A sensitive, simple, and accurate HPLC method was developed for the assay of telmisartan in human plasma. Using naproxen as internal standard, the assay involved liquid-liquid extraction of the compound from acidified plasma into organic solvent and reversed-phase chromatography with fluorescence detection. The assay was shown to be linear from 0.5 to 1000 ng/mL. In 24 healthy volunteers, the plasma concentrations of the drug were determined after a single oral dose of 160 mg.
Clinical pharmacy laboratory, Hua Shan Hospital, Fudan University, Shanghai, PR China.
J
J
Shen
Z
Z
Jiao
Z D
ZD
Li
X J
XJ
Shi
M K
MK
Zhong
D000328:Adult; D057912:Angiotensin II Type 2 Receptor Blockers; D001562:Benzimidazoles / Q000097:blood* / Q000493:pharmacokinetics*; D001565:Benzoates / Q000097:blood* / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D000077333:Telmisartan
D057912:Angiotensin II Type 2 Receptor Blockers; D001562:Benzimidazoles; D001565:Benzoates; D007202:Indicators and Reagents; D000077333:Telmisartan