[Polymorphism Analysis of the CSN1S2 Gene Digested.].
PCR-RFLP was applied to analyze the polymorphism of CSN1S2 gene in 170 goats that comprised of five goat breeds, namely Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat. A 310 bp -long PCR product was digested with Alw26I and demonstrated polymorphism in five goat populations that were all at Hardy-Weinberg equilibrium (P>0.05). For Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat, gene heterozygosity/effective allele gene number/Shaanon information entropy /Polymorphism information content were 0.1589/1.1889/0.2955/0.1463, 0.4114/1.6981/0.6017/0.5171, 0.1653/1.1980/0.3046/0.1516, 0646/1.0691/0.1463/ 0.0625, 0.0541/1.0572/0.1270/ 0.0526, respectively. According to the heredity diversity indexes described above of the five goat breeds, Guanzhong dairy goat had the most abundant heredity diversity and showed high polymorphism, and Xinong Saanen dairy goat and Shaannan white goat were inferior, while Angora goat and Boer goat had the lowest genetic variability.
Lan
Xian-Yong
XY
College of Animal Science and Technology, Northwest A & F University, China.
Chen
Hong
H
Zhang
Run-Feng
RF
Tian
Yi
Y
Lei
Chu-Zhao
CZ
Pan
Chuan-Ying
CY
Luo
Jun
J
Hu
Shen-Rong
SR
D000483:Alleles; D000818:Animals; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length