Bone marrow donor routine HLA typing identified a novel B*55 allele, B*5517, confirmed by allele-specific DNA cycle sequencing.
In this article, we report the identification of a new human leukocyte antigen-B allele in a sample that was tested in our routine typing for volunteer bone marrow donors. This novel allele officially designed B*5517 was found in a male donor of Bavarian Caucasoid origin (Laboratory code 150113). The search for unrelated bone marrow donors was initiated by the Aktion Knochenmarkspende Bayern. In comparison with the common B*5501 allele, B*5517 differs at three nucleotide positions all located in exon 3, at position 379 (C-->G), 419 (T-->A), and 420 (A-->C), resulting in two amino acid substitutions at codons 127 and 140. The amino acid 127, leucine, is changed to valine, and the amino acid 140, also a leucine, is replaced by tyrosine in B*5517.
Labor of Immunogenetics, University Munich, Munich, Germany. kwitter@med.uni-muenchen.de
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Witter
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Volgger
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Zahn
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Scholz
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Sala
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Albert
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000379:methods; D004247:DNA / Q000737:chemistry; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014019:Tissue Donors; D044465:White People
D015235:HLA-B Antigens; C503972:HLA-B*55:17 antigen; D009841:Oligonucleotides; D004247:DNA