Studies on nucleolar organiser regions (AgNors) in selected spontaneous and transplantable animal tumors.
Behaviour of argyrophilic nucleolus organising regions (AgNOR) was estimated in various types of spontaneous and transplantable tumors in animals. The studies were performed on spontaneous epithelial and mesenchymal tumors, malignant and non-malignant, as well as transplantable tumors: Morris hepatoma, mammary gland carcinoma and Yoshid sarcoma. The examinations were made on paraffin sections, using silver-staining method according to Ploton et al. Quantitative assessment was made with computer-aided microscopic image analysis system Multi-Scan Base V.8 for Windows, coupled with Carl Zeiss microscope. It was demonstrated that AgNOR index reflects malignancy of the tumor, since it increases clearly in cancers and sarcomas, both spontaneous and transplantable. The highest AgNOR index--0.13--was noted in the group of spontaneous tumors in epithelial malignant tumors, and in the group of transplantable tumors in mesenchymal tumors (Yoshid sarcoma) it was 0.15. Classification of the studied spontaneous and transplantable tumors into groups of the same histogenesis, though phenotypically different, was aimed at demonstration of the increasing tendency of AgNOR index.
Dzimira
S
S
Department of Veterinary Pathology, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Agricultural Academy, Norwid Street 35, 50-375 Wrocław, Poland. dzimira@ozi.ar.wroc.pl
Madej
J A
JA
Nowak
M
M
D000818:Animals; D004283:Dog Diseases / Q000473:pathology; D004285:Dogs; D005260:Female; D007091:Image Processing, Computer-Assisted; D015674:Mammary Neoplasms, Animal / Q000473:pathology; D009369:Neoplasms / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining / Q000662:veterinary*