Determination of bisphenol-A in water by semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent.
A highly sensitive method for the determination of bisphenol-A in water with semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent has been developed. The labeling reaction was carried out at 70 degrees C for 20 min in borate buffer (pH 9.5). The derivative eluted at 11.6 min on a reversed-phase column with methanol-water (78:22, v/v) at a flow-rate of 0.2 ml/min. The fluorescence was monitored at 308 nm for excitation and 410 nm for emission. The detection limit (S/N = 3) was 10 fmol per injection. The labeling yield was about 95%.
Tsuruta
Yasuto
Y
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan. tsuruta@fupharm.fukuyama-u.ac.jp
Inoue
Hirofumi
H
Fukunaga
Keiko
K
Munemura
Sayaka
S
Ozaki
Misa
M
Ohta
Masaya
M
Matsuura
Fumito
F
D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D005456:Fluorescent Dyes; D005618:Fresh Water / Q000032:analysis; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis*; D010797:Phthalimides / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013441:Sulfinic Acids / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010636:Phenols; D010797:Phthalimides; D013441:Sulfinic Acids; D014874:Water Pollutants, Chemical; C410032:2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride; C006780:bisphenol A; D000098826:Bisphenol A Compounds