Effect of salivary gland extract of Leishmania vector, Lutzomyia longipalpis, on leukocyte migration in OVA-induced immune peritonitis.
Salivary gland extracts (SGE) from Lutzomyia longipalpis potentate L. major infection by inducing a Th2 immune response. However, the effect of SGE on the effector phase of immune response is not known. Herein, we demonstrate that SGE inhibited neutrophil migration in ovalbumin (OVA)-induced peritonitis in immunized mice. SGE pretreatment of mice inhibited OVA-induced CD4+ and CD8+ T lymphocyte migration. The OVA-induced production of TNF-alpha, IL-1beta and leukotriene B4 (LTB4), neutrophil chemotactic mediators in this model, were inhibited by SGE. On the other hand, SGE enhanced production of IL-10 and IL-4. In naive mice, SGE also blocked LTB4-induced neutrophil migration, but not that induced fMLP. Moreover, co-incubation of LTB4 (but not fMLP, TNF-alpha and MIP-1alpha) with SGE inhibited the ability of LTB4 to induce neutrophil migration in vivo and in vitro. Altogether, the results suggest that SGE has anti-inflammatory properties that are associated with inhibition of TNF-alpha and LTB4 production and/or with the neutrophil chemotactic activity of LTB4. The effectiveness of SGE in inhibiting neutrophil migration and inflammatory mediators release in a Th1 immune inflammatory response model reinforces the need for isolation of the compounds responsible for these activities, which could be used as prototypes for the development new anti-inflammatory drugs.
Monteiro
Marta C
MC
Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Nogueira
Luciana G
LG
Almeida Souza
Adelson A
AA
Ribeiro
José M C
JM
Silva
João S
JS
Cunha
Fernando Q
FQ
D000818:Animals; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D007891:Leishmania; D051379:Mice; D009504:Neutrophils / Q000276:immunology / Q000473:pathology*; D010538:Peritonitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D011576:Psychodidae / Q000276:immunology* / Q000469:parasitology*; D012469:Salivary Glands / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology
D016207:Cytokines; D014020:Tissue Extracts