Accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms.
A microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. Representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (Yersinia enterocolitica, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli) were printed on a Brain Heart Infusion (BHI) agar slide using a contact micro-spotting robotic system. Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples.
Al-Khaldi
Sufian F
SF
Division of Microbiological Studies, OPDFB, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740-3855, USA. Sufian.Al-Khaldi@cfsan.fda.gov
Mossoba
Magdi M
MM
Ismail
Ashraf A
AA
Fry
Fred S
FS
D001419:Bacteria / Q000302:isolation & purification*; D016971:Citrobacter freundii / Q000302:isolation & purification; D016000:Cluster Analysis; D016972:Enterobacter cloacae / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005516:Food Microbiology; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000302:isolation & purification; D046228:Microarray Analysis / Q000379:methods*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013997:Time Factors; D015008:Yersinia enterocolitica / Q000302:isolation & purification