Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma.
Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet been identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama University, Japan.
Hidetsugu
H
Tsujigiwa
Hitoshi
H
Nagatsuka
Phuu Pwint
PP
Han
Mehmet
M
Gunduz
Chong Huat
CH
Siar
Shinichiro
S
Oida
Noriyuki
N
Nagai
D000564:Ameloblastoma / Q000235:genetics*; D053523:Amelogenin; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053523:Amelogenin; D003746:Dental Enamel Proteins