[Genetic characteristics of human pathogenic Borrelia isolated from Ixodes trianguliceps Bir. and Ixodes pavlovskyi Pom].
To support the results of typing the amplicon of the spacer site 5S-23S of pRNA by restriction fragment length polymorphism (RFLP) assay, the isolates from the ticks I. trianguliceps and I. pavlovskyi underwent sequenation of rrf (5S)-rrl (23S) spacer to make a comparative analysis of derived nucleotide sequences with the DDBJ/EMBL/ GenBank databases. For this purpose, the authors used six isolates (three imago and three nymphal) from I. trianguliceps and three isolates from adult hungry I. pavlovskyi females from the Borrelia museum of the Laboratory of Infection Transmitters, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences. In the natural foci of the Perm Region, in the tick I. trianguliceps the same genetic variants of B. afzelii VS461 and B. garinii NT29 was found to occur as in I. persulcatus and small mammals, the reservoir Borrelia hosts. Borreliae previously detected in the tick I. pavlovskyi belong to B. garinii 20047.
V  V
V
Nefedova
E I
EI
Korenberg
I A
IA
Fadeeva
N B
NB
Gorelova
D000818:Animals; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D026863:Ixodidae / Q000382:microbiology*; D008969:Molecular Sequence Data; D030721:Nymphaea / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S