[Expression and genetic immunization of hantaan virus G2 recombinant adenovirus].
OBJECTIVE
To express hantaan virus(HTNV) envelope glycoprotein G(2) recombinant adenovirus(Adeno-G(2)) in vero E6 cells and explore its property of inducing immune response.


METHODS
Vero E6 cells were infected with the HTNV Adeno-G(2) (100 MOI). The expression of Adeno-G(2) in the infected Vero E6 cells was detected by IFA. BALB/c mice were immunized with HTNV Adeno-G(2), then the immune response to Adeno-G(2) was tested by ELISA, microcell-culture neutralizing experiment and lymphocyte proliferation test (MTT colorimetry).


RESULTS
IFA detection showed the expression of Adeno-G(2) in the infected Vero E6 cells. The titer of specific antibody was 1:40; The low-titer neutralization antibody was also detected. But the lymphocyte proliferation reaction was not notable.


CONCLUSIONS
The HTNV Adeno-G(2) can stimulate BALB/c mice to develop specific humoral immune response instead of specific cell-mediated immunity. This study provides the experimental basis for the development of gene engineering vaccine of HFRS.
Department of Microbiology, Faculty of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Xing-an
XA
Wu
Lan
L
Yu
Gang
G
Hu
Fang-lin
FL
Zhang
Wen-tao
WT
Bai
Meng-yuan
MY
Shi
Zhi-kai
ZK
Xu
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D018063:Hantaan virus / Q000235:genetics* / Q000276:immunology*; D007114:Immunization; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C090197:glycoprotein G2, Hantavirus