Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus.
Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 microl greatly reduced the number of isolates requiring induction.
University of North Carolina Hospitals, University of North Carolina School of Medicine, Campus Box 7525, Chapel Hill, NC 27599-7525, USA. mbmiller@unch.unc.edu
Melissa B
MB
Miller
Heather
H
Meyer
Elaine
E
Rogers
Peter H
PH
Gilligan
D001426:Bacterial Proteins / Q000235:genetics*; D003030:Coagulase / Q000378:metabolism; D006801:Humans; D007841:Latex Fixation Tests; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins / Q000378:metabolism*; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C487789:mecA protein, Staphylococcus aureus; D010068:Oxacillin