[Angiogenesis and regulatory factors in rats with BPH induced by testosterone].
OBJECTIVE
To study angiogenesis and regulatory factors in the proliferated prostatic tissues of Sprague Dawley (SD) rats with BPH induced by testosterone.


METHODS
Sixteen castrated SD rats, aged 8 weeks and weighing 200 approximately 250 g, were equally randomized into a model group and a control group, and the BPH model was established by subcutaneous injection of testosterone. Immunohistochemistry and MIAS (micro-image analysis system) were used to test the manifestations of MVD (microvessel density), VEGF (vascular endothelium growth factor), flk-1, endostatin, MMP-2 (matrix metalloproteinase-2) and TIMP-2 (tissue inhibitor of metalloproteinase-2) in the prostatic tissues of both the model and the control groups. Multiple linear regression with the stepwise method was adopted to analyze the data.


RESULTS
The manifestations of MVD, VEGF, flk-1, MMP-2, MMP-2/TIMP-2 and VEGF/endostatin in the model group were higher, while that of endostatin was lower than in the control group (P < 0.01), and the manifestation of TIMP-2 showed no statistical difference between the two groups. The regression analysis indicated that MVD was positively correlated to VEGF, VEGF/endostatin and MMP-2/TIMP-2 (r = 0.974, 0.986, 0.982, P < 0.05) and negatively correlated to endostatin (r = - 0.975, P < 0.05) .


CONCLUSIONS
Testosterone could induce BPH in SD rats by increasing MVD and promoting the multiplication of vascular endothelial cells after regradation of basement membrane.
Department of Urology, Affiliated Hospital of Chengdu University of TCM, Chengdu, Sichuan 610072, China. shaoji93@163.com
Ji-Chun
JC
Shao
Yi
Y
Wang
Shu-Wu
SW
Zhang
De-Kang
DK
Luo
De-Gui
DG
Chang
Xian-Qi
XQ
Wu
Min
M
Tang
Zi-Ming
ZM
He
D000818:Animals; D004195:Disease Models, Animal; D043169:Endostatins / Q000096:biosynthesis; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D009389:Neovascularization, Pathologic / Q000139:chemically induced* / Q000378:metabolism; D011467:Prostate / Q000098:blood supply* / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000139:chemically induced* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis
D043169:Endostatins; D042461:Vascular Endothelial Growth Factor A; D019716:Tissue Inhibitor of Metalloproteinase-2; D013739:Testosterone; D040301:Vascular Endothelial Growth Factor Receptor-2; D020778:Matrix Metalloproteinase 2