High-throughput evaluation of antioxidant and pro-oxidant activities of polyphenols with thymidine protection assays.
A recently reported high-throughput screening strategy has been applied to the rapid selection of new water-soluble antioxidants that display strong protective activities. Based on a competitive immunoassay, a triple-screening procedure was used to evaluate the ability of different compounds to protect thymidine under different oxidative stresses. The pro-oxidant effect of norbadione A in the presence of iron was observed, while some pulvinic acid derivatives proved strongly protective during gamma radiolysis, UV irradiation, and Fenton-like oxidation.
Service de Marquage Moléculaire et de Chimie Bio-Organique, DBJC/DSV, CEA Saclay, 91191 Gif sur Yvette Cedex, France.
Stéphane
S
Meunier
Mikaël
M
Hanédanian
Marine
M
Desage-El Murr
Stéphanie
S
Nowaczyk
Thierry
T
Le Gall
Serge
S
Pin
Jean-Philippe
JP
Renault
Didier
D
Boquet
Christophe
C
Créminon
Charles
C
Mioskowski
Frédéric
F
Taran
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002264:Carboxylic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D005419:Flavonoids / Q000737:chemistry* / Q000502:physiology; D007118:Immunoassay; D007783:Lactones / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry* / Q000494:pharmacology; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry*; D010648:Phenylacetates / Q000737:chemistry; D059808:Polyphenols; D011794:Quercetin / Q000737:chemistry; D017382:Reactive Oxygen Species; D013936:Thymidine / Q000737:chemistry*
D000975:Antioxidants; D002264:Carboxylic Acids; D005419:Flavonoids; D007783:Lactones; D016877:Oxidants; D010636:Phenols; D010648:Phenylacetates; D059808:Polyphenols; D017382:Reactive Oxygen Species; C464564:norbadione A; D011794:Quercetin; D015107:4-Butyrolactone; C492654:pulvinic acid; D013936:Thymidine