Susceptibility testing of Pseudomonas aeruginosa by the Vitek 2 system: a comparison with Etest results.
P. aeruginona infections need accurate antimicrobial susceptibility data, as treatment mainly relies on antibiotic efficiency in debilitated patients. Vitek 2, a popular automated susceptibility testing method, was compared with Etest to assess its reliability on 150 Belgian P. aeruginonas isolates. Vitek 2 and Etest exhibited a high degree of concordance, but some discrepancies in clinical category were evident for cefepime (high minor and borderline very major error rate) and for piperacillin/tazobactam (high very major error rate). Vitek 2 appears to yield valuable information to the clinician concerning the antimicrobials amikacin, ceftazidime, ciprofloxacin and meropenem, in the setting of pseudomonas infection. For cefepime and piperacillin/tazobactam, a confirmatory testing by means of disk diffusion is worth considering.
UZ Gasthuisberg, KULeuven, Dept. Microbiologie, Herestraat 49, 3000 Leuven. veroniek.saegeman@uz.leuven.ac.be
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Saegeman
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Huynen
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Colaert
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Melin
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Verhaegen
D001431:Bacteriological Techniques; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic