Induction method of tyrosine kinase A-mediated cell death in rat pheochromocytoma.
Nerve Growth Factor (NGF) is a neurotrophic factor that prevents apoptosis in neuronal progenitor cells. In rat pheochromocytoma (PC12) cells, tyrosine kinase A receptor (TrkA) mediates neurotrophic or protective effects, while p75 neurotrophin receptor (p75NTR) functions as a death receptor. We have determined whether TrkA mediates any cytotoxic effect. Following serum deprivation, TrkA expression increased 2.2-fold and apoptosis began with expression of Bax proapoptotic protein. Application of NGF halved cell viability but this was reversed by K252a, the TrkA inhibitor. These results confirmed the paradoxical cytotoxic effect of neurotrophic NGF via TrkA in PC12 cells following serum deprivation.
Department of Neurology, Kangnam General Hospital Public Crop, Seoul, Korea.
Jin-Young
JY
Ahn
Lami
L
Kang
Hyun-Jeong
HJ
Kim
Hyejin
H
Kang
Sung-Hoon
SH
Kim
Je-Yong
JY
Kim
Seung Ki
SK
Kim
Kyu-Chang
KC
Wang
Byung Kyu
BK
Cho
Kwang-Woo
KW
Lee
Jae-Kyu
JK
Roh
Manho
M
Kim
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002227:Carbazoles / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D026121:Indole Alkaloids; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000378:metabolism / Q000473:pathology; D051381:Rats; D020917:Receptor, trkA / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002227:Carbazoles; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D020932:Nerve Growth Factor; C049985:staurosporine aglycone; D020917:Receptor, trkA