[Isolation and Sequence Analysis of the Xa21 Gene Wxon II Homologus from Different Species of Wild Rice in Yunnan.].
The Xa21 gene previously cloned from the wild rice species Oryzae longistaminata confers broad-spectrum resistance to rice leaf blight caused by different strains of Xanthomonas oryzae pv. oryzae. Here we attempted to determine the existence of Xa21 homologs in other wild rice species and rice cultivars and the sequence differences between the homologs. We synthesized specific primers based on the reported Xa21 sequence to amplify homologs of the gene exon II from several rice cultivars and three wild rice species in Yunnan Province, China. The fragments cloned from various types of O. rufipogon Griff from Jinghong and Yuanjiang, Yunnan Province, were highly homologous to the reported Xa21 gene exon II. However, the fragment was not found in O. officinalis Wall. and O. meyeriana Baill. Sequence analysis suggested that differences in nucleotides were located randomly in the fragments we cloned.
Key Laboratory of Biotechnology, Yunnan Academy of Agricultural Science, Kunming 650091, China, Email: junqqj@126.com.
Jun
J
Qian
Zai-Quan
ZQ
Cheng
Ming-Zhi
MZ
Yang
Ji-Mei
JM
Liu
Cheng-Jun
CJ
Wu
Xing-Qi
XQ
Huang
D002681:China; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D017421:Sequence Analysis; D014974:Xanthomonas
D010940:Plant Proteins