Liquid chromatographic-mass spectrometric assay for simultaneous pyrimethamine and sulfadoxine determination in human plasma samples.
We present a liquid chromatographic-mass spectrometric assay for the simultaneous determination of sulfadoxine and pyrimethamine in human plasma samples. Sample clean-up was achieved by adding acetonitrile for protein precipitation. Gradient elution in only 10 min resulted in high throughput capability. Tandem mass spectrometric detection in multiple reaction monitoring was used for quantification. The developed analytical approach was successfully validated and was applied in the pharmacokinetic evaluation of the bioavailability between two sulfadoxine/pyrimethamine formulations available on the Eastern African market, using a cross-over design.
Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000, Gent, Belgium.
Bart A
BA
Sinnaeve
Tineke N
TN
Decaestecker
Peter G
PG
Risha
Jean-Paul
JP
Remon
Chris
C
Vervaet
Jan F
JF
Van Bocxlaer
D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011739:Pyrimethamine / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013413:Sulfadoxine / Q000097:blood* / Q000493:pharmacokinetics
D000962:Antimalarials; D013413:Sulfadoxine; D011739:Pyrimethamine