Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold.
The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins of unknown function or are classified as conserved hypothetical proteins. We have determined the three-dimensional structure of one of these, the rv0216 gene product, which has been shown to be essential for M. tuberculosis growth in vivo. The structure exhibits the greatest similarity to bacterial and eukaryotic hydratases that catalyse the R-specific hydration of 2-enoyl coenzyme A. However, only part of the catalytic machinery is conserved in Rv0216 and it showed no activity for the substrate crotonyl-CoA. The structure of Rv0216 allows us to assign new functional annotations to a family of seven other M. tuberculosis proteins, a number if which are essential for bacterial survival during infection and growth.
Castell
Alina
A
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Sweden.
Johansson
Patrik
P
Unge
Torsten
T
Jones
T Alwyn
TA
Bäckbro
Kristina
K
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D002470:Cell Survival; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D017510:Protein Folding; D017386:Sequence Homology, Amino Acid; D014376:Tuberculosis / Q000382:microbiology
D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; C010701:crotonyl-coenzyme A