Effects of pure n-alkanes and crude oil on bacterial phospholipid classes and molecular species determined by electrospray ionization mass spectrometry.
Phospholipids are major components of bacterial membrane. Furthermore, the growth in vitro on xenobiotics such as n-alkanes, aromatic compounds or alkanols bring about to a bacterial membrane adaptive response. Concerning this work, we studied the membrane lipid composition of a hydrocarbon-degrading gram-positive bacterium (Corynebacterium sp.) on a soluble substrate and we detected four different phospholipid classes: phosphatidylglycerol, phosphatidylinositol, cardiolipin and acyl phosphatidylglycerol. In addition, a study of the lipid composition was performed after an in vitro culture on either pure n-alkane or crude oil. The growths on such hydrophobic substrates showed major qualitative and quantitative modifications. In the case of a growth on either heneicosane or crude oil, an increase of odd-numbered fatty acids was observed. Furthermore, the phospholipid polar head group composition was highly influenced by the crude oil addition. These modifications were, respectively, interpreted as the consequence of hydrocarbon assimilation and membrane fluidity adaptation. Finally, Corynebacterium sp. was taken back on the initial ammonium acetate substrate in order to determine its restoration abilities after a petroleum contamination.
Mazzella
Nicolas
N
Laboratoire de Chimie Analytique de l'Environnement, UMR 6171, IFR PMSE 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
Molinet
Josiane
J
Syakti
Agung Dhamar
AD
Barriol
Alexandre
A
Dodi
Alain
A
Bertrand
Jean-Claude
JC
Doumenq
Pierre
P
D000473:Alkanes / Q000494:pharmacology*; D003352:Corynebacterium / Q000737:chemistry* / Q000187:drug effects*; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D010578:Petroleum; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000473:Alkanes; D004952:Esters; D005227:Fatty Acids; D010578:Petroleum; D010743:Phospholipids