Antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin.
Antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. Of the 120 isolates Pseudomonas spp. (55.8%) was the predominant group followed by Acinetobacter spp. (14.17%), Flavobacterium spp. (10.83%), Achromobacter spp. (10%), Burkholderia cepacia (3.3%), Agrobacterium/radiobacter (2.5%), Moraxella spp. (1.7%), Aeromonas hydrophila (1.7%). Over 80% of the isolates were resistant to one or more antibiotics and the highest resistance was found for chloramphenicol, ampicillin, colistin and sulfamethizole (60%, 55%, 50% and 47.5%, respectively). Strains with multiple antibiotic resistance (MAR) represented 55% of isolates and the most resistant organism belonged to the genus Pseudomonas. Of 40 randomly selected strains, 27 (67.5%) had antibacterial activity towards one or more indicators. This activity, found in a high percentage in the genus Pseudomonas (92%), emerged mainly against closely related microorganisms. Several producers were active also against Escherichia coli, Salmonella, Listeria monocytogenes and Staphylococcus aureus. Forty-six percent of the isolates harboured 1 to 5 plasmids with molecular weights ranging from 2.1 to 41.5 MDa.
Department of Biomedical Sciences, University of Modena and Reggio E., Via Campi 287, 41100 Modena, Italy.
Patrizia
P
Messi
Elisa
E
Guerrieri
Moreno
M
Bondi
D000900:Anti-Bacterial Agents / Q000032:analysis; D001419:Bacteria; D024881:Drug Resistance, Bacterial; D004784:Environmental Monitoring; D008900:Mineral Waters / Q000382:microbiology*; D012987:Soil; D014871:Water Microbiology; D014881:Water Supply
D000900:Anti-Bacterial Agents; D008900:Mineral Waters; D012987:Soil