Analysis of potential and free furfural compounds in milk-based formulae by high-performance liquid chromatography. Evolution during storage.
A simple and reproducible HPLC-diode array detection method for the qualitative and quantitative analysis of potential and free furfural compounds (5-hydroxymethyl-2-furaldehyde, HMF; 2-furaldehyde, F; 2-furyl methyl ketone, FMC; and 5-methyl-2-furaldehyde, MF) in milk-based formulae was developed and validated. The method showed good linearity with determination coefficients over 0.999. The limits of detection and quantification were acceptable for all furfurals. The relative standard deviations (RSDs) for repeatability and reproducibility were <4.28. Recoveries in all furfurals were between 94.5 and 98.7%. In addition, we report the evolution over shelf life of furfural compound levels in an experimental powder formula for pregnant women stored at 25 and 37 degrees C from production until 15 months.
Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, E-08028 Barcelona, Spain.
Jorge L
JL
Chávez-Servín
Ana I
AI
Castellote
M Carmen
MC
López-Sabater
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000032:analysis*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005662:Furaldehyde