Appraisal of the extent of chronic allograft vasculopathy in animal models: proposition of a standardized micromorphometric method.
Arterial obstruction due to intimal myproliferation is an outcome used for investigating chronic allograft vasculopathy in animal models. Since harvested tissue may be distorted while processing, discrepancy may happen in the measure of the obstruction. We propose a standardized micromorphometric method for calculating the percentage of obstruction with eliminating the variability in the shape of the vessel cross-section. The mathematical adjustment avoids the overestimation of the percentage of obstruction and improves accuracy.
Department of Cardiothoracic Surgery, Transplant Immunology Lab, University of Stanford, Stanford, CA, USA.
Géraldine
G
Rousvoal
Marc A
MA
Rousseau
Mona G
MG
Flores
Dominic C
DC
Borie
D000818:Animals; D001011:Aorta / Q000473:pathology / Q000637:transplantation; D002908:Chronic Disease; D004195:Disease Models, Animal; D006083:Graft Occlusion, Vascular / Q000473:pathology*; D006084:Graft Rejection / Q000473:pathology*; D008853:Microscopy / Q000379:methods* / Q000592:standards*; D008954:Models, Biological; D051381:Rats; D014184:Transplantation, Homologous