Complete genomic sequence of the temperate bacteriophage PhiAT3 isolated from Lactobacillus casei ATCC 393.
The complete genomic sequence of a temperate bacteriophage PhiAT3 isolated from Lactobacillus (Lb.) casei ATCC 393 is reported. The phage consists of a linear DNA genome of 39,166 bp, an isometric head of 53 nm in diameter, and a flexible, noncontractile tail of approximately 200 nm in length. The number of potential open reading frames on the phage genome is 53. There are 15 unpaired nucleotides at both 5' ends of the PhiAT3 genome, indicating that the phage uses a cos-site for DNA packaging. The PhiAT3 genome was grouped into five distinct functional clusters: DNA packaging, morphogenesis, lysis, lysogenic/lytic switch, and replication. The amino acid sequences at the NH2-termini of some major proteins were determined. An in vivo integration assay for the PhiAT3 integrase (Int) protein in several lactobacilli was conducted by constructing an integration vector including PhiAT3 int and the attP (int-attP) region. It was found that PhiAT3 integrated at the tRNAArg gene locus of Lactobacillus rhamnosus HN 001, similar to that observed in its native host, Lb. casei ATCC 393.
Institute of Molecular Medicine and Department of Life Science, National Tsing Hua University, Hsinchu 30043, Taiwan, ROC.
Ta-Chun
TC
Lo
Tsung-Chieh
TC
Shih
Chao-Fen
CF
Lin
Hung-Wen
HW
Chen
Thy-Hou
TH
Lin
D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D016679:Genome, Viral; D019426:Integrases / Q000235:genetics; D007780:Lacticaseibacillus casei / Q000821:virology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D016662:Virus Integration
D004279:DNA, Viral; D014764:Viral Proteins; D012343:RNA, Transfer; D019426:Integrases