Circadian oscillations of RPCH gene expression in the eyestalk of the crayfish Cherax quadricarinatus.
The RPCH and beta-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C. quadricarinatus RPCH and beta-actin mRNAs, as cDNAs, were quantified every 3h by RT-PCR. Single cosinor analysis supports the notion of beta-actin and RPCH mRNA circadian behavior in animals subjected to 12h:12h light/dark regimes. In constant darkness RPCH mRNA concentration changes to ultradian cycles.
Posgraduate School of Biological Sciences, Facultad de Ciencias, Universidad Nacional Autónoma de México, D.F., 04510 México, Mexico.
Francisco
F
Martínez-Pérez
Samuel
S
Zinker
Guadalupe
G
Aguilar
Jesús
J
Valdés
Hugo
H
Aréchiga
D000818:Animals; D003400:Astacoidea / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001683:Biological Clocks / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D005123:Eye / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives*
D009842:Oligopeptides; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid