Characterization of a functional P2X(7)-like receptor in cerebellar granule neurons from P2X(7) knockout mice.
The presence of ionotropic P2X(7) receptor has been studied in mice brain from wild type and P2X(7) receptor knockout animals. Western blot and immunocytochemical assays show the presence of a protein containing the P2X(7) immunogenic epitopes in the brain of knockout model. Reverse transcriptase polymerase chain reaction experiments demonstrate the absence of the disrupted sequence, but other sequences of P2X(7) specific mRNA expression have been detected. Functional calcium imaging experiments in cultured granule neurons from P2X(7) knockout cerebella show the existence of a functional P2X(7)-like receptor that keeps some of the properties of the genuine receptor.
Department of Biochemistry, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain.
Jesús
J
Sánchez-Nogueiro
Patricia
P
Marín-García
M Teresa
MT
Miras-Portugal
D000818:Animals; D002531:Cerebellum / Q000166:cytology*; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7
C546469:P2rx7 protein, mouse; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7