Anti-Trichinella antibodies detected in chronically infected horses by IFA and Western blot, but not by ELISA.
In the Balkan countries, where trichinellosis is a re-emerging zoonosis, it is of great importance to determine Trichinella infection prevalence among the major hosts, including horses. One method for monitoring prevalence is serological surveillance; however, the validity of serological methods in horses is not well understood. The dynamics of anti-Trichinella IgG production and circulating excretory/secretory (ES) antigens were investigated in three horses experimentally-infected with Trichinella spiralis. Horses were slaughtered at 32 week post infection (p.i.). Low worm burdens were found in all three animals. Anti-Trichinella IgG was detected up to 32 weeks p.i. by an indirect immunofluorescence assay (IFA) and by Western blot (Wb), but not by ELISA. The ELISA test detected antibodies for only a short period of time (up to 18 weeks p.i. using ES antigen or up to 20 weeks p.i. using tyvelose-BSA antigen). The presence of circulating muscle larvae ES antigen in sera of infected horses was observed by dot blot from the 4th week p.i. up to the 32nd week p.i.
Institute for the Application of Nuclear Energy (INEP), Banatska 31b, 11080 Belgrade, Serbia and Montenegro. sofronic@inep.cp.yu
Lj
Lj
Sofronic-Milosavljevic
N
N
Ilic
M
M
Djordjevic
M
M
Savic
A
A
Gruden-Movsesijan
K
K
Cuperlovic
K D
KD
Murrell
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000737:chemistry; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015801:Helminth Proteins / Q000737:chemistry; D006601:Hexoses / Q000737:chemistry; D006734:Horse Diseases / Q000097:blood / Q000276:immunology / Q000469:parasitology*; D006736:Horses; D014059:Tongue / Q000469:parasitology; D014234:Trichinella / Q000254:growth & development / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D015022:Yugoslavia
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D006601:Hexoses; C097419:excretory-secretory antigen, Trichinella; C009616:tyvelose