Permanent genetic tagging of podocytes: fate of injured podocytes in a mouse model of glomerular sclerosis.
Injured podocytes lose differentiation markers. Therefore, the true identity of severely injured podocytes remains unverified. A transgenic mouse model equipped with a podocyte-selective injury induction system was established. After induction of podocyte injury, mice rapidly developed glomerulosclerosis, with downregulation of podocyte marker proteins. Proliferating epithelial cells accumulated within Bowman's space, as seen in collapsing glomerulosclerosis. In this study, the fate of injured podocytes was pursued. Utilizing Cre-loxP recombination, the podocyte lineage was genetically labeled with lacZ in an irreversible manner. After podocyte injury, the number of lacZ-labeled cells, which were often negative for synaptopodin, progressively declined, correlating with glomerular damage. Parietal epithelial cells, but not lacZ-labeled podocytes, avidly proliferated. The cells proliferating within Bowman's capsule and, occasionally, on the outer surface of the glomerular basement membrane were lacZ-negative. Thus, when podocytes are severely injured, proliferating parietal epithelial cells migrate onto the visceral site, thereby mimicking proliferating podocytes.
Institute of Medical Sciences, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Takako
T
Asano
Fumio
F
Niimura
Ira
I
Pastan
Agnes B
AB
Fogo
Iekuni
I
Ichikawa
Taiji
T
Matsusaka
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000378:metabolism; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology; D005923:Glomerulosclerosis, Focal Segmental / Q000473:pathology*; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007678:Kidney Glomerulus / Q000473:pathology*; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008954:Models, Biological; D008957:Models, Genetic; D050199:Podocytes / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D019076:Transgenes
D018076:DNA, Complementary