Seizure-induced changes of mineralocorticoid and glucocorticoid receptors in the hippocampus in seizure sensitive gerbils.
Abnormal corticosteroid hormone levels during stress and resultant mineralocorticoid receptor (MR)/glucocorticoid receptor (GR) imbalance enhance the vulnerability of specific hippocampal neurons. In the present study, we investigated the distribution of MR and GR in seizure resistant (SR) and seizure sensitive (SS) gerbils, and observed the seizure-induced changes of MR and GR in the hippocampus of SS gerbils using immunohistochemistry and western blot analysis. MR and GR immunoreactivities were higher in the SS pre-seizure gerbils than that in SR gerbils. In the SR gerbils, the immunodensity of GR was high compared to that of MR. The changes of MR and GR immunoreactivities were significant in the stratum pyramidale of the hippocampal CA1 region and the infrablade of the dentate gyrus after seizure on-set. MR immunoreactivity in the CA1 region was significantly increased at 12h after seizure on-set, thereafter MR immunoreactivity was decreased. MR immunoreactivity in the dentate gyrus was decreased time-dependently after seizure on-set. GR immunoreactivity was decreased in the CA1 region and dentate gyrus time-dependently after seizure on-set. At 12h after seizure on-set, differences in MR and GR immunodensity diminished in the CA1 region and dentate gyrus. This imbalance of MR and GR immunoreactivity in these regions may be associated with seizure generation in the Mongolian gerbil, which is a hereditary seizure model.
Department of Anatomy, College of Medicine, Hallym University, 1 Okchon-dong, Chunchon 200-702, South Korea.
In Koo
IK
Hwang
Yoon-Bok
YB
Lee
Ki-Yeon
KY
Yoo
Tae-Cheon
TC
Kang
Dong-Woo
DW
Kim
Won-Kook
WK
Moon
Sang Moo
SM
Kim
Yang-Seok
YS
Oh
Heon-Soo
HS
Sohn
Moo Ho
MH
Won
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D005260:Female; D005849:Gerbillinae; D006624:Hippocampus / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012640:Seizures / Q000378:metabolism*; D013997:Time Factors
D011965:Receptors, Glucocorticoid