In tuberculous pleural effusions, activated neutrophils undergo apoptosis and acquire a dendritic cell-like phenotype.
Tuberculous pleuritis usually shows lymphocytic preponderance, but neutrophils are also present. Therefore, pleuritis is a good model for the study of neutrophil fate at sites of active Mycobacterium tuberculosis infection. We have previously demonstrated in vitro that M. tuberculosis-induced neutrophil apoptosis involves p38 mitogen protein kinase activation through Toll-like receptor 2. Herein, we demonstrate that, in tuberculous pleuritis, neutrophil apoptosis increases together with the expression of Toll-like receptor 2 and phosphorylated p38 (p-p38) kinase. In addition, receptors associated with activation/apoptotis (CD11b, CD64, tumor necrosis factor receptor, and Fas ligand) are up-regulated, together with a loss of CD16 expression. However, neutrophils express CD86, CD83, and major histocompatibility complex class II antigens, acquiring dendritic cell (DC) characteristics. Therefore, the cytokine milieu in the pleural space may influence signaling pathways on activated neutrophils, thereby inducing apoptosis and inhibiting their proinflammatory capacity, as well as allowing them acquire DC characteristics that influence the immune response.
Aleman
Mercedes
M
Departamento de Inmunologia, Instituto de Investigaciones Hematologicas, Academia Nacional de Medicina, Buenos Aires, Argentina.
de la Barrera
Silvia S
SS
Schierloh
Pablo L
PL
Alves
Leandro
L
Yokobori
Noemi
N
Baldini
Matias
M
Abbate
Eduardo
E
Sasiain
Maria C
MC
D015703:Antigens, CD / Q000097:blood; D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D003713:Dendritic Cells / Q000473:pathology* / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D009504:Neutrophils / Q000473:pathology* / Q000502:physiology; D010641:Phenotype; D010996:Pleural Effusion / Q000097:blood*; D012016:Reference Values; D014397:Tuberculosis, Pulmonary / Q000097:blood*
D015703:Antigens, CD