5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats.
Pre-treatment with 5-aminosalicylic acid (5-ASA) significantly reduced sperm-shape abnormalities in endosulfan-treated rats. The number of abnormal sperm in the epididymis was markedly increased by endosulfan treatment but pre-treatment with 5-ASA kept these values close to normal. Treatment with 5-ASA at a dose of 25 mg/kg bw was more effective in reducing sperm-shape abnormality and sperm count than at a dose of 50 mg/kg bw. Endosulfan significantly increased the level of lactate dehydrogenase (LDH) in rats but a marked decrease was observed upon pre-treatment with 25 mg/kg bw 5-ASA. Changes in plasma testosterone levels were not significantly correlated with 5-ASA pre-treatment but histopathological analysis of seminiferous tubules and Leydig cells showed significant protection from endosulfan-induced tissue damage such as necrosis. The population of Sertoli cells increased and the lumen of the seminiferous tubules contained a greater number of spermatids. There was a corresponding increase in the number of Leydig cells. A curative study with 5-ASA showed a similar protection from endosulfan-induced toxicity and cellular damage, but the extent of protection was significantly lower.
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 576104, India.
Amardeep
A
Jaiswal
Vipan Kumar
VK
Parihar
M
M
Sudheer Kumar
S D
SD
Manjula
B R
BR
Krishnanand
Ravindranath
R
Shanbhag
M K
MK
Unnikrishnan
D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004726:Endosulfan / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D019804:Mesalamine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology*; D013739:Testosterone / Q000097:blood
D000931:Antidotes; D007306:Insecticides; D013739:Testosterone; D019804:Mesalamine; D007770:L-Lactate Dehydrogenase; D004726:Endosulfan