Protective role of lycopene on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats.
The aim of this study was to investigate the possible protective role of lycopene on cisplatin (CP)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches. Adult male Sprague-Dawley rats were randomly divided into four groups. The control group received physiological saline; animals in cisplatin group received only cisplatin; pre-treatment group received a 10-day of lycopene before administration of cisplatin while animals in post-treatment group received a 5-day of lycopene following administration of cisplatin. Cisplatin (7 mg kg(-1)) was intraperitoneally (i.p.) injected as a single dose and lycopene (4 mg kg(-1)) was administered by gavage in corn oil. Traits of reproductive organs; sperm characteristics, testicular histological findings, plasma testosterone levels and the testicular tissue oxidative status were determined. Administration of cisplatin to rats decreased sperm concentration (p < 0.05) and sperm motility (p < 0.001), increased total abnormal sperm rates (p < 0.05) as compared with the control group. While a marked normalization was achieved only in sperm concentration with lycopene in pre-treatment group, significant normalizations were achieved in the sperm concentration, sperm motility, total abnormal sperm rates in post-treatment group. No significant differences in levels of testosterone were observed among all groups. An increase in testes malondialdehyde concentrations (p < 0.05) and glutathione peroxidase activities (p < 0.001) were detected while significant decreases in glutathione levels (p < 0.001) in cisplatin alone group when compared to control group. While pre-treatment with lycopene restoring only malondialdehyde concentrations, its post-treatment caused normalization in both malondialdehyde and glutathione levels when compared with the cisplatin alone group. On the other hand, significant increases were determined in GSH-Px activities in all experimental groups when compared with the control group. Although the mechanism is not clear, the results from this experimental study suggest that the lycopene have a possible protective effect against cisplatin-induced spermiotoxicity, effect of giving lycopene after cisplatin being superior to the giving it before cisplatin.
Ateşşahin
Ahmet
A
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Firat University, 23119 Elaziğ, Turkey. aatessahin@hotmail.com
Karahan
Izzet
I
Türk
Gaffari
G
Gür
Seyfettin
S
Yilmaz
Seval
S
Ceribaşi
Ali Osman
AO
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002945:Cisplatin / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects / Q000254:growth & development; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D000077276:Lycopene; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress; D011467:Prostate / Q000187:drug effects / Q000254:growth & development; D051381:Rats; D017207:Rats, Sprague-Dawley; D012669:Seminal Vesicles / Q000187:drug effects / Q000254:growth & development; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood
D000970:Antineoplastic Agents; D000975:Antioxidants; D002338:Carotenoids; D013739:Testosterone; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005978:Glutathione; D002945:Cisplatin; D000077276:Lycopene