Generation of cell adhesive substrates using peptide fluoralkyl surface modifiers.
Previous studies reported on the delivery of vitamin E to the surface of a polycarbonate polyurethane (PCNU) to produce antioxidant surfaces, using a bioactive fluorinated surface modifer (BFSM). In the current report, a cell adhesive peptide sequence was coupled to the BFSM, and when blended into PCNU, generated a cell adhesive substrate. An NH2-GK*GRGD-CONH2 peptide sequence (referred to as RGD) with a dansyl label (*) on the lysine residue was coupled via the N-terminal to a BFSM precursor molecule. The resulting RGD BFSM was purified and the pmol peptide/mg BFSM value was assayed by amino acid quantification. The migration of the RGD BFSM in a PCNU blend was confirmed by X-ray photoelectron spectroscopy analysis. U937 macrophage-like cells and human monocytes were seeded onto the PCNU and blends of PCNU with non-bioactive fluorinated surface modifier or the RGD BFSM, in order to study the cell response. Both U937 cells and human monocytes adhered in greater numbers to the RGD BFSM substrate when compared to unmodified PCNU or the blend of PCNU with the non-bioactive fluorinated surface modifying macromolecule substrate. The study demonstrated a novel approach for the introduction of peptides onto the surface of polymers by modifying the surface from within the polymer as opposed to the use of cumbersome post-surface modification techniques. The generation of a peptide substrate points to the possibility of producing complex bioactive surfaces using various peptide BFSMs or pharmaceuticals simultaneously to manipulate cell functions.
Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ont., Canada.
Mark J
MJ
Ernsting
Geneviève C
GC
Bonin
Meilin
M
Yang
Rosalind S
RS
Labow
J Paul
JP
Santerre
D000269:Adhesives / Q000737:chemistry; D000975:Antioxidants / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002855:Chromatography, Thin Layer; D004577:Electron Probe Microanalysis / Q000379:methods; D006018:Glycols / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D010649:Phenylalanine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties; D020298:U937 Cells
D000269:Adhesives; D000975:Antioxidants; D001672:Biocompatible Materials; D006018:Glycols; D046911:Macromolecular Substances; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; C047554:polytetramethylene glycol; D010649:Phenylalanine; C047981:arginyl-glycyl-aspartic acid; D008239:Lysine