Measurement of the propelled liquid by isolated hamster ileum as a parameter to evaluate peristalsis.
We present a method to measure the volume of liquid propelled by peristaltic movements of isolated hamster ileum as a novel means to assess peristaltic activity. The oral and aboral ends of the dissected ileum were attached to cannulas fixed horizontally. The application of intraluminal pressure by raising the level of liquid in the bottle connected to the oral end evoked peristalsis and intermittent propulsion of the intraluminal liquid. The inhibition of intrinsic neurons by tetrodotoxin stopped propulsion; this indicated that the liquid propulsion was correlated with neuron-regulated peristalsis. The volume of liquid propelled by one complete peristaltic movement was significantly greater than that by incomplete peristalsis, whereas recordings of pressure changes were indistinguishable. Inhibitors of nitric oxide synthase decreased the volume of liquid propelled by peristaltic movements, suggesting a role of nitrergic neurons in peristalsis. Our data show that the method described above might be suitable for analyzing peristalsis.
Shiina
Takahiko
T
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
Shimizu
Yasutake
Y
Suzuki
Yuji
Y
Nikami
Hideki
H
Takewaki
Tadashi
T
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D010528:Peristalsis / Q000187:drug effects / Q000502:physiology*; D011312:Pressure; D012965:Sodium Chloride / Q000378:metabolism* / Q000493:pharmacokinetics; D013779:Tetrodotoxin / Q000494:pharmacology
D004791:Enzyme Inhibitors; D019335:Nitroarginine; D009569:Nitric Oxide; D018738:Hexamethonium; D013779:Tetrodotoxin; D012965:Sodium Chloride; D001285:Atropine; D019001:Nitric Oxide Synthase; D000109:Acetylcholine