[Changes in the permeability of amphibian embryo envelope for fluorochromes under the action of ultrasound].
It was determined whether high-frequency ultra sound can change the permeability of gray toad Bufo bufo and grass frog Rana temporaria under the action of high-frequency ultrasound. The changes in the permeability of embryonic envelope were assessed by using slowly penetrating fluorochromes ANS, FDA, and fluorescein. It was found that the ultrasound of 0.88 MHz and 0.4 - 0.7 W/cm2 intensity increased the permeability of amphibian embryonic envelope for ANS and FDA, whereas the ultrasound of 2.64 MHz and the same intensity increased that for fluorescein with the retention of low permeability for FDA. Embryos continued the normal development after treatment with ultrasound under these conditions.
Mel'nikova
E V
EV
Uteshev
V K
VK
Pashovkin
T N
TN
Sokolova
A A
AA
Iashin
V A
VA
Karnaukhov
V N
VN
Gakhova
E N
EN
D000818:Animals; D002023:Bufo bufo; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000502:physiology*; D004625:Embryo, Nonmammalian; D019793:Fluorescein / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D010539:Permeability; D011896:Rana temporaria; D014465:Ultrasonics
D005456:Fluorescent Dyes; D019793:Fluorescein