Identification of a universal Group B streptococcus vaccine by multiple genome screen.
Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens.
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Domenico
D
Maione
Immaculada
I
Margarit
Cira D
CD
Rinaudo
Vega
V
Masignani
Marirosa
M
Mora
Maria
M
Scarselli
Hervé
H
Tettelin
Cecilia
C
Brettoni
Emilia T
ET
Iacobini
Roberto
R
Rosini
Nunzio
N
D'Agostino
Lisa
L
Miorin
Scilla
S
Buccato
Massimo
M
Mariani
Giuliano
G
Galli
Renzo
R
Nogarotto
Vincenzo
V
Nardi-Dei
Vincenzo
V
Nardi Dei
Filipo
F
Vegni
Claire
C
Fraser
Giuseppe
G
Mancuso
Giuseppe
G
Teti
Lawrence C
LC
Madoff
Lawrence C
LC
Paoletti
Rino
R
Rappuoli
Dennis L
DL
Kasper
John L
JL
Telford
Guido
G
Grandi
D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D019295:Computational Biology; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D007112:Immunity, Maternally-Acquired; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins; D010587:Phagocytosis; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D014611:Vaccination
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D009895:Opsonin Proteins; C414463:SIP protein, Streptococcus group B; D022541:Streptococcal Vaccines