Pathologic alterations detected in acute pancreatitis induced by sodium taurocholate in rats and therapeutic effects of curcumin, ciprofloxacin and metronidazole combination.
OBJECTIVE
Secondary bacterial infections and free radical injury have been known to play an important role in the pathogenesis and clinical outcome of acute pancreatitis. Despite the therapy models developed in recent years, the mortality rate is still reported to be higher than expected. The objective of this study therefore was to investigate the effectiveness of ciprofloxacin and metronidazole combination and curcumin together in the treatment of acute pancreatitis.


METHODS
Acute pancreatitis was induced in rats by sodium taurocholate (n = 60). Starting 6 h after the induction of acute pancreatitis, groups I and II were injected 200 mg/kg ciprofloxacin and 500 mg/kg metronidazole intraperitoneally every 12 h for 6 days. Groups II and III received 100 mg/kg curcumin since day 20 prior to the initiation of acute pancreatitis. On day 6, animals of all groups were killed. Blood and tissue samples were taken for biochemical, pathologic and bacteriologic examination.


RESULTS
No statistical difference in the treatment groups versus the non-treatment group has been detected in the pancreatic tissue on the basis of histopathological scoring results. Prevalences of bacterial translocation were significantly lower in the treatment groups (groups I-III) than in the non-treatment group (group IV) (p < 0.001, p < 0.001, p < 0.05, respectively). Serum amylase, lipase, malon dialdehyde and nitric oxide (except for nitric oxide level in group I), levels of groups I, II and III were significantly lower than those of group IV (p < 0.05).


CONCLUSIONS
The administration of ciprofloxacin and metronidazole in combination and curcumin in acute pancreatitis failed to provide a preventive effect on the occurrence of tissue injury, whereas free radical injury and prevalence of bacterial translocation were reduced significantly.
Gülçubuk
Ahmet
A
Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey. agulcubuk@yahoo.com
Sönmez
Kivilcim
K
Gürel
Aydin
A
Altunatmaz
Kemal
K
Gürler
Nezahat
N
Aydin
Seval
S
Oksüz
Lütfiye
L
Uzun
Hafize
H
Güzel
Ozlem
O
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D018988:Bacterial Translocation / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology / Q000627:therapeutic use*; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008795:Metronidazole / Q000494:pharmacology / Q000627:therapeutic use*; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid; D016896:Treatment Outcome
D000890:Anti-Infective Agents; D000975:Antioxidants; D004791:Enzyme Inhibitors; D008795:Metronidazole; D013656:Taurocholic Acid; D002939:Ciprofloxacin; D003474:Curcumin