Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
[reaction: see text] We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha(C)U). An efficient photoligation was produced by photoirradiation of an ODN containing alpha(C)U at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new and efficient way to synthesize branched ODNs.
The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa.
Masayuki
M
Ogino
Yoshinaga
Y
Yoshimura
Akio
A
Nakazawa
Isao
I
Saito
Kenzo
K
Fujimoto
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry; D013698:Templates, Genetic
C509569:5-cyanovinyldeoxyuridine; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine