[Study on bone marrow transplantation camouflaged with methoxy polyethylene glycol].
To explore the effect of bone marrow camouflaged with methoxy polyethylene glycol (mPEG) on allogeneic bone marrow transplantation, 60 BALB/c(H-2d) mice were randomly divided into 3 groups after irradiation by 8.0 Gy of (60)Co gamma ray. A group was given RPMI 1640 0.5 ml in tail vein. B group was infused with the bone marrow cells (1 x 10(7)) mixed with the spleen cells (1 x 10(7)) of donor 615(H-2k) mice. C group was transplanted with same dose cells, which were camouflaged with mPEG before infusion. Severity GVHD was determined by total manifestation of mice, survival rate, survival time and histo-pathological microscopy, and engraftment of allogeneic bone marrow was evaluated by chromosome examination. The results showed that 75% mice in B group had severe adverse manifestations, such as hunched posture, diarrhea and loss of hair. Occurrence of the same adverse manifestations in C group was 35% and significantly lower than that in B group (P <or= 0.05). The mean survival time and survival rate of C group were 16.1 days and 50% respectively, which were higher than those of B group (12.1 days and 20%). White blood cells in peripheral blood of C group mice recovered more quickly than that in B group mice. The number of GVHD grad III in B group was 59%, whereas in C group was 20% (chi(2) = 3.844, P = 0.050); allogeneic bone marrow was successfully engrafted in B and C groups. In conclusion, GVHD of allogeneic bone marrow transplantation can be alleviated by bone marrow cells camouflaged with mPEG, and such transplantation may be able to prolong survival time and increase survival rate.
Department of Pediatrics, General Hospital of Chinese PLA, Beijing, 100853, China.
Hui
H
Long
Suo-Qin
SQ
Tang
Xiao-Fei
XF
Zhang
D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000379:methods*; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology / Q000401:mortality / Q000517:prevention & control*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D011897:Random Allocation; D016019:Survival Analysis; D015996:Survival Rate; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation
D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol