[Alogenic islet transplantation on the rat liver after alogenic dendritic cells injection in the thymus].
BACKGROUND
The major indication for pancreas or islet transplantation is diabetes mellitus type I. This process has to supply the insulin necessity keeping glucose under control.


OBJECTIVE
We studied alogenic islet transplantation on the rat liver, Wistar (RT1u) to Lewis (RT1(1)) as a recipient. Control group (n = 8) and dendritic cell group (n = 9) respectively with injection of Hanks solution and dendritic cells in the thymus before islet transplantation.


METHODS
With the method of isolation and purification of the islets we obtained both in the control group 3637 +/- 783,3 islets with purity of 85 +/- 3,52% and dendritic cell group 3268 +/- 378 islets with purity of 87 +/- 4,47%. The dendritic cells were retrieved from the spleen and we obtained 3,34 x 105+/-1,16 cells. Diabetes was induced by i.v. streptozotocin.


RESULTS
Control group the transplantation of 3637 +/- 783,3 islets in the rat liver normalized glucose test, 7,21 +/- 0,57 mmol/L in the second post-operative day. Acute rejection came in the 10 postoperative day with significantly increase of glucose test. Dendritic cell group, the transplantation of 3258 +/- 378 islets in the rat liver, normalized the glucose test was 9,3 +/- 2,85 mmoL/L in the second postoperative day. From the 4th postoperative day to 10th postoperative day the glucose test increase significantly showing an early acute rejection.


CONCLUSIONS
The injection of dendritic cells in the thymus before alogenic islet transplantation in the rat liver lead to an early acute rejection.
Departamento de Cirurgia, Universidade de Cambridge, Inglaterra.
Eleazar
E
Chaib
Apostolos
A
Papalois
Ingrid G M
IG
Brons
Roy Y
RY
Calne
D000818:Animals; D002469:Cell Separation / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005260:Female; D006085:Graft Survival; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods*; D008297:Male; D010180:Pancreatectomy; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D013311:Streptozocin; D013950:Thymus Gland; D014185:Transplantation, Isogeneic
D013311:Streptozocin