Ergosterol peroxide from flowers of Erigeron annuus L. as an anti-atherosclerosis agent.
Flowers of Erigeron annuus L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H2O. Repeated silica gel and ODS column chromatography of the EtOAc fraction led to the isolation of a sterol, through activity-guided fractionation, using ACAT inhibitory activity measurements. From the physico-chemical data, including NMR, MS, and IR, the chemical structure of the compound was determined to be an ergosterol peroxide (1), which has been isolated for the first time from this plant. This compound exhibited hACAT-1 and Lp-PLA2 inhibitory effects, with inhibitory values of 51.6 +/- 0.9 and 51.7 +/- 1.2%, at a treatment concentration of 0.23 mM.
Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Dong-Hyun
DH
Kim
Sung Je
SJ
Jung
In-Sik
IS
Chung
Youn-Hyung
YH
Lee
Dae-Keun
DK
Kim
Sung-Hoon
SH
Kim
Byoung-Mog
BM
Kwon
Tae-Sook
TS
Jeong
Mi-Hyun
MH
Park
Nak-Sul
NS
Seoung
Nam-In
NI
Baek
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D001161:Arteriosclerosis / Q000188:drug therapy*; D019659:Asteraceae / Q000737:chemistry*; D002784:Cholesterol; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D051381:Rats; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; D010741:Phospholipases A; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol