Expression of the human melanocortin-2 receptor in different eukaryotic cells.
The human melanocortin-2 receptor (hMC2R) is mainly present in the adrenal cortex and has been difficult to express in heterologous cells. The hMC2R fused to the EGFP at its C-terminus has been stably transfected in the murine M3 melanoma and HEK293 cells. In the M3 cells, the hMC2R-EGFP was well-addressed to the cell membrane and functional whereas in the HEK293 cells, the hMC2R-EGFP was retained intracellularly. These results suggest that some specific factors, missing in cells, which do not express any melanocortin receptor, are involved in the correct addressing of the hMC2R to the cell membrane.
Rached
Mohamed
M
INSERM, U418/INRA UMR 1245, IFR Laënnec, Lyon F-69005, France.
El Mourabit
Haquima
H
Buronfosse
Anna
A
Blondet
Antonine
A
Naville
Danielle
D
Begeot
Martine
M
Penhoat
Armelle
A
D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000378:metabolism; D044103:Receptor, Melanocortin, Type 2 / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D044104:Receptor, Melanocortin, Type 3 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D044105:Receptor, Melanocortin, Type 4 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D012333:RNA, Messenger; D044103:Receptor, Melanocortin, Type 2; D044104:Receptor, Melanocortin, Type 3; D044105:Receptor, Melanocortin, Type 4; D011993:Recombinant Fusion Proteins