Immunoscreening protocols for the identification of clinically useful antibodies and antigens.
The antigen-antibody interaction is a powerful tool for the immuno-screening of several diseases, including cancer and genetic disorders. The high specificity of monoclonal antibodies (mAbs) enables them to target antigens and form complexes that can be detected with enzymes, radionuclides, fluorescent dyes or other markers. The antibody molecule, which has an antigen binding site, can be used as an intact molecule or as a fragment, for example, F(ab)(2), Fab, Fv or scFv. Similarly, the antigen can also be varied. In this review, immuno-screening techniques that can be used to detect clinically relevant antibody-antigen interactions will be discussed.
Mukerjee
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Novopharm Biotech, Inc., 10246 Parkdale Ave., San Diego, CA 92126, USA.
McKnight
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Glassy
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