Valuation of exfoliative cytology as prediction factor in oral mucosa lesions.
The aim of this study was immunolabeling oncoproteins Ck14, p53, p21 and Bcl-2 in order to evaluate their expression in premalignant and malignant stomatological lesions in oral epithelial, and to compare this expression with exfoliative cytology alterations in the same patients. It was studied biopsies and cytologies of 13 subjects with oral lichen planus, with or without Human Papilloma Virus (HPV), leukoplakia and squamous cell carcinoma clinically diagnosed and confirmed by anatomopathological studies. The oral lichen planus lesion presented binuclei orange cells; and in leukoplakia lesions only orange stained was observed; meanwhile koilocytes, inflammatory cells, enlarge nuclear volume and pathogenic microorganisms were observed in the HPV infections and squamous cells carcinoma (SCC). The Ck14, p53, p21 and Bcl-2 proteins were found modified in the leukoplakia, oral lichen planus and cancer. Cytological alterations and positive immunolabeling or over-expression of Ck14 cytokeratine in the upper epithelial stratus should be indicator of malignant transformations as doing subsequence exams.
Brunotto
Mabel
M
Facultad de Odontología, Universidad Nacional de Córdoba, Argentina. mbruno@odo.unc.edu.ar
Zárate
Ana María
AM
Cismondi
Adriana
A
Fernández
María del Carmen
Mdel C
Noher de Halac
Rita Inés
RI
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000378:metabolism; D016022:Case-Control Studies; D003581:Cytodiagnosis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis; D007972:Leukoplakia, Oral / Q000737:chemistry* / Q000378:metabolism; D017676:Lichen Planus, Oral / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D027383:Papillomaviridae / Q000302:isolation & purification; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis
D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D007633:Keratins; D016283:Proto-Oncogene Proteins p21(ras)