In vivo import of plastocyanin and a fusion protein into developmentally different plastids of transgenic plants.
Transgenic tomato plants that constitutively express a foreign plastocyanin gene were used to study protein transport in different tissues. Normally expression of endogenous plastocyanin genes in plants is restricted to photosynthetic tissues only, whereas this foreign plastocyanin protein is found to be present in all tissues examined. The protein is transported into the local plastids in these tissues and it is processed to the mature size. We conclude that plastids of developmentally different tissues are capable of importing precursor proteins that are normally not found in these tissues. Most likely such plastids, though functionally and morphologically differentiated, have similar or identical protein import mechanisms when compared to the chloroplasts in green tissue.
de Boer
D
D
Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, NL 3584 CH Utrecht, the Netherlands.
Cremers
F
F
Teertstra
R
R
Smits
L
L
Hille
J
J
Smeekens
S
S
Weisbeek
P
P
D002736:Chloroplasts / Q000235:genetics; D035264:Flowers / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D018087:Plastids / Q000235:genetics*; D010970:Plastocyanin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
D011498:Protein Precursors; D010970:Plastocyanin