A model for studying epithelial attachment and morphology at the interface between skin and percutaneous devices.
Percutaneous devices are indispensable in modern medicine, yet complications from their use result in significant morbidity, mortality, and cost. Bacterial biofilm at the device exit site accounts for most infections in short-term devices. We hypothesize that advanced biomaterials can be developed that facilitate attachment of skin cells to percutaneous devices, forming a seal to preclude bacterial invasion. To study the skin/biomaterial interface systematically, we first identified biomaterials with physical properties compatible with histological processing of skin. Second, we developed an organ culture system to study skin response to implants. Organ cultures implanted with porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] or polytetrafluoroethylene (PTFE) could easily be evaluated histologically with preservation of the skin/biomaterial interface. Epithelial cells migrated down the cut edges of the biomaterial in a pattern seen in marsupialization of percutaneous devices in vivo. This in vitro model maintains skin viability and allows histologic evaluation of the skin/biomaterial interface, making this a useful, inexpensive test-bed for studies of epidermal attachment to modified biomaterials.
Department of Medicine (Dermatology), University of Washington, Seattle, Washington 98195-6524, USA.
Negar G
NG
Knowles
Yuko
Y
Miyashita
Marcia L
ML
Usui
Andrew J
AJ
Marshall
Annalisa
A
Pirrone
Kip D
KD
Hauch
Buddy D
BD
Ratner
Robert A
RA
Underwood
Philip
P
Fleckman
John E
JE
Olerud
D000279:Administration, Cutaneous; D001672:Biocompatible Materials; D002404:Catheterization / Q000295:instrumentation*; D002448:Cell Adhesion / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008954:Models, Biological; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D001672:Biocompatible Materials