Generation of a Bmp2 conditional null allele.
Bone morphogenetic proteins (Bmp's) are known to play many important roles in embryogenesis. In addition, recent data from human genetic studies has revealed that Bmp's also have important functions in maintenance of the adult phenotype and aging. The original Bmp2 germline null allele resulted in lethality at embryonic day 7.0-10.5 due to malformation of the amnion/chorion and cardiac malformations. Because the early embryonic lethality of the Bmp2 germline null allele hinders further investigation into Bmp2 function at later stages, we generated a Bmp2 conditional null allele. Using gene targeting in mouse embryonic stem (ES) cells, we introduced LoxP sites upstream and downstream of Bmp2 exon 3 that encodes the mature peptide. Our results indicate that the Bmp2 conditional null allele is a true conditional null that encodes wildtype activity and reverts to a null allele after cre recombinase-induced recombination.
Ma
Lijiang
L
Alkek Institute of Biosciences and Technology, Texas A&M System Health Science Center, Houston, TX 77030, USA.
Martin
James F
JF
D000483:Alleles; D000818:Animals; D001287:Attachment Sites, Microbiological / Q000235:genetics; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000172:deficiency* / Q000235:genetics*; D002678:Chimera; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005838:Genotype; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D011995:Recombination, Genetic / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000172:deficiency* / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases