Genes involved in cyclic lipopeptide production are important for seed and straw colonization by Pseudomonas sp. strain DSS73.
Survival in natural bulk soil and colonization of sugar beet seeds and barley straw residues were determined for Pseudomonas sp. strain DSS73 and Tn5 mutants in amsY (encoding a peptide synthetase involved in production of the cyclic lipopeptide amphisin) and gacS (encoding the sensory kinase of the two-component GacA/GacS regulatory system). No differences in survival or growth in response to carbon amendment (citrate) were observed in bulk soil. However, both mutants were impaired in their colonization of sugar beet seeds and barley straw residues by an inoculum established in the bulk soil. The two mutants had comparable colonization phenotypes, suggesting that amphisin production is more important for colonization than other gacS-controlled traits.
Section of Genetics and Microbiology, Department of Ecology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Tommy H
TH
Nielsen
Ole
O
Nybroe
Birgit
B
Koch
Michael
M
Hansen
Jan
J
Sørensen
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D027461:Beta vulgaris / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D001467:Hordeum / Q000382:microbiology; D008074:Lipoproteins / Q000096:biosynthesis; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012639:Seeds / Q000382:microbiology*; D012988:Soil Microbiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D008074:Lipoproteins; D010456:Peptides, Cyclic; D014157:Transcription Factors; C438267:amphisin; C074165:lemA protein, bacterial; D010453:Peptide Synthases