[Transcriptional repression of pltZ on pyoluteorin ABC transporter of Pseudomonas sp. M18].
A pltZ gene involved in negatively regulating pyoluteorin (Plt) biosynthesis, and an ABC (ATP-binding cassette) transporter involved in pyoluteorin secretion and itself resistance, were identified downstream of Plt biosynthesis gene cluster in Pseudomonas sp. M18. The ABC transporter gene pltH'-lacZ translational and transcriptional fusion expression plasmids, pHZLF and pHZCF, were constructed using promoter probe vector pME6015 and pME6522 respectively, and then were introduced into the wild-type strain of Pseudomonas sp. M18 and the pltZ mutant strain M18Z. The chromosomal pltZ disruption mutant gave rise to 3.7-8.4-fold enhancement of translational pltH'-'lacZ fusion expression and 2.8-7.4-fold enhancement of transcriptional pltH'-'lacZ fusion expression compared with those in the wild-type strain M18. These results suggested that pltZ can repress transcription of Plt ABC transporter, and it can indirectly regulate Plt biosynthesis negatively through the Plt ABC transporter system.
School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China. xqhuang66@126.com
Xian-qing
XQ
Huang
Yi-he
YH
Ge
Xue-hong
XH
Zhang
Yu-quan
YQ
Xu
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010636:Phenols / Q000378:metabolism*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism*; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin