Expression of 15-lipoxygenase-1 in human nasal epithelium: its implication in mucociliary differentiation.
15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here, we investigated the relation between 15-LO-1 expression and the differentiation of human nasal epithelium. In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in normal human nasal epithelial cell time-dependently increased, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 at 1 ng/ml concentration time-dependently induced 15-LO-1 expression. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In murine nasal mucosa, the expression of leukocyte type-12-LO, a functional equivalent of 15-LO-1, reduced after postnatal day 7. Our findings suggest that 15-LO-1 is related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of human nasal epithelium.
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea. ydrhinol@yumc.yonsei.ac.kr
Kyung-Su
KS
Kim
Hee-Sun
HS
Chun
Joo-Heon
JH
Yoon
Jeung Gweon
JG
Lee
Joo-Hwan
JH
Lee
Jong-Bum
JB
Yoo
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D002923:Cilia / Q000201:enzymology; D003470:Culture Media; D015870:Gene Expression; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D051379:Mice; D009077:Mucins / Q000235:genetics; D009093:Mucus / Q000166:cytology; D009297:Nasal Mucosa / Q000166:cytology / Q000201:enzymology*; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation
D003470:Culture Media; D005819:Genetic Markers; C105059:MUC8 protein, human; D009077:Mucins; D015847:Interleukin-4; D014212:Tretinoin; D001093:Arachidonate 15-Lipoxygenase