Further structural analysis of GnRH complexes with metal ions.
MALDI-TOF mass spectrometry, 1H NMR spectrometry, the continuous variation method and molecular modeling by MM3 calculation confirmed our earlier studies showing that gonadotropin-releasing hormone (GnRH) forms complex with copper(II) ion with the binding ratio 1:1. The copper(II) complex formed at physiological pH has a square planar configuration and GnRH complexes with nickel(II) and cobalt(II) ions are less stable than that of copper(II).
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. k.kochman@ifzz.pan.pl
Kazuhiko
K
Nakamura
Masato
M
Kodaka
Ibrahim M
IM
El-Mehasseb
Alina
A
Gajewska
Hiroaki
H
Okuno
Ewa
E
Ochwanowska
Bozena
B
Witek
Henryk
H
Kozłowski
Kazimierz
K
Kochman
D000818:Animals; D003300:Copper / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019216:Metals, Heavy; D011522:Protons; D007987:Gonadotropin-Releasing Hormone; D003300:Copper; D009532:Nickel