Direct measurement of osmotic pressure of glycosaminoglycan solutions by membrane osmometry at room temperature.
Articular cartilage is a hydrated soft tissue composed of negatively charged proteoglycans fixed within a collagen matrix. This charge gradient causes the tissue to imbibe water and swell, creating a net osmotic pressure that enhances the tissue's ability to bear load. In this study we designed and utilized an apparatus for directly measuring the osmotic pressure of chondroitin sulfate, the primary glycosaminoglycan found in articular cartilage, in solution with varying bathing ionic strength (0.015 M, 0.15 M, 0.5 M, 1 M, and 2 M NaCl) at room temperature. The osmotic pressure (pi) was found to increase nonlinearly with increasing chondroitin sulfate concentration and decreasing NaCl ionic bath environment. Above 1 M NaCl, pi changes negligibly with further increases in salt concentration, suggesting that Donnan osmotic pressure is negligible above this threshold, and the resulting pressure is attributed to configurational entropy. Results of the current study were also used to estimate the contribution of osmotic pressure to the stiffness of cartilage based on theoretical and experimental considerations. Our findings indicate that the osmotic pressure resulting from configurational entropy is much smaller in cartilage (based on an earlier study on bovine articular cartilage) than in free solution. The rate of change of osmotic pressure with compressive strain is found to contribute approximately one-third of the compressive modulus (H(A)(eff)) of cartilage (Pi approximately H(A)(eff)/3), with the balance contributed by the intrinsic structural modulus of the solid matrix (i.e., H(A) approximately 2H(A)(eff)/3). A strong dependence of this intrinsic modulus on salt concentration was found; therefore, it appears that proteoglycans contribute structurally to the magnitude of H(A), in a manner independent of osmotic pressure.
Chahine
Nadeen O
NO
Musculoskeletal Biomechanics Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York 10027, USA.
Chen
Faye H
FH
Hung
Clark T
CT
Ateshian
Gerard A
GA
D000818:Animals; D001703:Biophysics / Q000379:methods*; D002356:Cartilage / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D019277:Entropy; D006025:Glycosaminoglycans / Q000737:chemistry*; D007477:Ions; D008954:Models, Biological; D009995:Osmosis; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011312:Pressure; D011509:Proteoglycans / Q000737:chemistry; D012492:Salts / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D006025:Glycosaminoglycans; D007477:Ions; D011108:Polymers; D011509:Proteoglycans; D012492:Salts; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D002809:Chondroitin Sulfates