Two-dimensional patterning of thin coatings for the control of tissue outgrowth.
Control of the precise location and extent of cellular attachment and proliferation, and of tissue outgrowth is important in a number of biomedical applications, including biomaterials and tissue engineered medical devices. Here we describe a method to control and direct the location and define boundaries of tissue growth on surfaces in two dimensions. The method relies on the generation of a spatially defined surface chemistry comprising protein adsorbing and non-adsorbing areas that allow control over the adsorption of cell-adhesive glycoproteins. Surface modification was carried out by deposition of thin acetaldehyde and allylamine plasma polymer coatings on silicon wafer and FEP substrates, followed by grafting of a protein resistant layer of poly(ethylene oxide). Spatially controlled patterning of the surface chemistry was achieved by masking during plasma polymerization. XPS and AFM were used to provide evidence of successful surface modifications. Adsorption of the extracellular matrix protein collagen I followed by tissue outgrowth experiments with bovine corneal epithelial tissue for up to 21 days showed that two-dimensional control over tissue outgrowth is achievable with our patterning method over extended time frames. The method promises to be an effective tool for use in a number of in vitro and in vivo applications.
Thissen
Helmut
H
CSIRO Molecular Science, Bayview Avenue, Clayton 3169 VIC, Australia. helmut.thissen@csiro.au
Johnson
Graham
G
Hartley
Patrick G
PG
Kingshott
Peter
P
Griesser
Hans J
HJ
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D003094:Collagen / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D003315:Cornea / Q000378:metabolism / Q000473:pathology; D004577:Electron Probe Microanalysis; D004847:Epithelial Cells / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering
D001672:Biocompatible Materials; D024042:Collagen Type I; D006023:Glycoproteins; D011108:Polymers; D011092:Polyethylene Glycols; D003094:Collagen; D012825:Silicon