Surface-sensitive polarized Raman spectroscopy of biological tissue.
In a two-layer diffusing medium, polarized light directly backscattering off the superficial layer will partially retain its sense of polarization, whereas deeper-probing light will be increasingly depolarized by diffusion. This effect has been studied in both elastic scattering and fluorescence contexts. We apply this method to Raman scattering in two two-layer models with a highly diffusing lower layer of glucose powder and an upper layer of either clear plastic or chicken skin. We employ detection of orthogonal polarization states to generate a Raman spectrum of only the superficial layer by combining the orthogonal signals.
The Institute of Optics, University of Rochester, Rochester, New York 14627, USA.
Zachary J
ZJ
Smith
Andrew J
AJ
Berger
D000818:Animals; D002645:Chickens; D005240:Feasibility Studies; D018613:Microscopy, Confocal / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D013059:Spectrum Analysis, Raman / Q000379:methods*