Near IR emitting isothiocyanato-substituted fluorophores: their synthesis and bioconjugation to monoclonal antibodies.
Two near IR emitting fluorophores, based on the phthalocyanine and naphthalocyanine chromophores, which also bear a single isothiocyanato group suitable for conjugation to proteins are reported; their utility as luminescent probes is demonstrated by conjugation to monoclonal antibodies and the ability of these conjugates to selectively bind cells bearing the relevant antigen.
University of Hull, Department of Chemistry and Clinical Biosciences Institute, Hull, UKHU6 7RX.
Wubiao
W
Duan
Karen
K
Smith
Huguette
H
Savoie
John
J
Greenman
Ross W
RW
Boyle
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D007211:Indoles / Q000737:chemistry; D054833:Isoindoles; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D017879:Isothiocyanates; C013647:phthalocyanine