[Antigenic analysis of the recombinant capsid protein of porcine circovirus type 2].
The nuclear localization signal (NLS)-defected capsid protein gene (dCap) of porcine circovirus type 2 (PCV2) was expressed firstly in Escherichia coli as a fusion protein with glutathione S-transferase (rGST-dCap protein). The purified rGST-dCap protein and NLS-defected Cap protein of PCV2 (rdCap protein) from the purified rGST-dCap protein reacted specifically with swine antiserum to PCV2. Furthermore, the obtained monoclonal antibodies (mAbs) to rdCap protein were shown to bind to PCV2 particles replicated in PK15 cell. MAbs to rdCap protein also revealed the neutralizing ability to PCV2 particles. These results demonstrate that rGST-dCap protein expressed in E. coli is folded correctly or at least partly, and all mAbs to rdCap protein possess the binding epitopes of PCV2 particle whereas mAbs 4C4,3F6 and 2G7 to rdCap protein keep the neutralization epitopes of PCV2 particle, showing a potential of rGST-dCap protein as a vaccine antigen or serodiagnostic reagent.
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
Shao-bin
SB
Shang
Ji-yong
JY
Zhou
Jian-xiang
JX
Wu
Qing-xin
QX
Chen
Hui
H
Gong
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase