Development of rapid immunochromatographic test with recombinant NcSAG1 for detection of antibodies to Neospora caninum in cattle.
An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Min
M
Liao
Shoufa
S
Zhang
Xuenan
X
Xuan
Guohong
G
Zhang
Xiaohong
X
Huang
Ikuo
I
Igarashi
Kozo
K
Fujisaki
D000034:Abortion, Veterinary / Q000097:blood* / Q000175:diagnosis; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D003048:Coccidiosis / Q000097:blood* / Q000175:diagnosis / Q000662:veterinary; D004285:Dogs; D005260:Female; D018688:Neospora / Q000276:immunology; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood* / Q000469:parasitology / Q000662:veterinary; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan