Comparative evaluation of intranasal and subcutaneous route of immunization for development of mucosal vaccine against experimental tuberculosis.
Activation of mucosal immunity in the respiratory tract is crucial for protection against respiratory infections. Whether the intranasal route of vaccination imparts better protection against pulmonary tuberculosis than that of subcutaneous vaccination remains a debatable issue. In this study, we have investigated the effect of the routes of immunization on the induction of immunoprotection against experimental tuberculosis employing mycobacterial culture filtrate proteins complexed with dimethyldioctadecylammonium bromide. Vaccination via intranasal and subcutaneous routes triggered immune activation in the spleen and cervical lymph node, while the former route of vaccination lead to higher antigen-specific lymphocyte proliferation, interferon-gamma, interleukin-12 and interleukin-4 responses in cervical lymph node and induction of antigen-specific IgA responses at mucosal level of the respiratory tract. Mice vaccinated via the intranasal route were found to be better protected against experimental tuberculosis particularly in lung compared to subcutaneous-immunized mice. These results emphasize the importance of the intranasal route vaccination in tuberculosis.
Giri
Pramod K
PK
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160 012 Chandigarh, India.
Sable
Suraj B
SB
Verma
Indu
I
Khuller
Gopal K
GK
D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control*; D014611:Vaccination
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D007070:Immunoglobulin A; D000644:Quaternary Ammonium Compounds; D032581:Tuberculosis Vaccines; C015831:dimethyldioctadecylammonium