Expression of cytokeratins 8 and 18 on Mallory body and oval cell in rats during hepatic fibrosis.
BACKGROUND
The aim of this study was to determine the induction and distribution of Mallory body (MB) and oval cells in carbon-tetrachloride (CCl4)-induced rat liver fibrosis.


METHODS
MBs and oval cells expressing cytokeratins (CKs) 8 and 18 were monitored by immuno-histochemistry and immunoblotting.


RESULTS
MBs were mainly detected within hepatocytes near the fibrotic areas, and oval cells were located along or in the fibrotic areas. Both MBs and oval cells increased in size and number in the development of fibrosis. At cirrhotic liver, most of the oval cells were located in the fibrous septa and around newly formed bile ductules. Moreover, as hepatic injuries developed into fibrosis, a much more prominent single band of CK18 was detected.


CONCLUSIONS
The occurrence and distribution of MB and oval cells in CCl4-induced rat liver fibrosis are reported. This represents the first CCl4 experimental in vivo model of MB induction, which will be useful for further investigations on the pathogenesis of MB.
Department of Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Won-Il
WI
Jeong
Da-Hee
DH
Jeong
Sun-Hee
SH
Do
Hai-Jie
HJ
Yang
Il-Hwa
IH
Hong
Seong-Joon
SJ
Chang
Dong-Wei
DW
Yuan
Dong-Mi
DM
Kwak
Tae-Hwan
TH
Kim
Yoo-Kyeong
YK
Kim
In-Seon
IS
Lee
Kyu-Shik
KS
Jeong
D000818:Animals; D002251:Carbon Tetrachloride; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D007124:Immunoenzyme Techniques; D002479:Inclusion Bodies / Q000378:metabolism* / Q000473:pathology; D007633:Keratins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D007633:Keratins; D002251:Carbon Tetrachloride