The macrolide roxithromycin impairs NADPH oxidase activation and alters translocation of its cytosolic components to the neutrophil membrane in vitro.
We have studied the interference of roxithromycin with NADPH oxidase, the key enzymatic system for oxidant production by human neutrophils. Roxithromycin alters the reconstitution of an active enzyme and impairs the translocation to the outer membrane of the cytosolic components p47-phox and p67-phox. Interestingly, in resting cells roxithromycin directly triggers the translocation of these factors without stimulating the oxidative burst.
Abdelghaffar
Houria
H
Université Hassan II, Faculté des Sciences et Techniques Mohammedia, Département de Biologie, Unité de Biotechnologies de l'Environnement et de la Santé, Mohammedia, Morocco.
Babin-Chevaye
Catherine
C
Labro
Marie-Thérèse
MT
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D019255:NADPH Oxidases / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology* / Q000276:immunology; D010750:Phosphoproteins / Q000187:drug effects / Q000378:metabolism; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D015575:Roxithromycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D010750:Phosphoproteins; C079789:neutrophil cytosol factor 67K; D015575:Roxithromycin; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D011493:Protein Kinase C