Sec13 induces genomic instability in U2OS cells.
Sec13p has been known as an endoplasmic reticulum-Golgi transport protein. Recently, it has also been shown to be required for the formation of septation in the fission yeast Schizosaccharomyces pombe. In the present study, we focused on the role of a human homolog of Saccharomyces cerevisiae SEC13, Sec13 protein during mitosis in U2OS cells. We found that the expression of Sec13 was constant throughout the cell cycle, and localized to the kinetochores at metaphase during mitosis. By using green fluorescent protein technology, we observed that Sec13 is required for evasion of mitotic arrest in response to spindle damage, leading to G1-like phase and apoptotic cell death. In addition, cells expressing exogenous Sec13 showed giant nuclei compared to endogenous ones in the absence of nocodazole. These results demonstrate that Sec13 is involved in the regulation of the metaphase/anaphase transition and may be functionally associated with mitotic machinery to maintain genomic stability during mitosis.
Sihn
Choong-Ryoul
CR
Department of Biology, College of Sciences, Kyung Hee University, Seoul 130-701, Korea.
Suh
Eun-Jung
EJ
Lee
Kee-Ho
KH
Kim
Sang Hoon
SH
D000705:Anaphase; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016193:G1 Phase; D042822:Genomic Instability; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008677:Metaphase; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D028861:Nuclear Pore Complex Proteins; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D029701:Saccharomyces cerevisiae Proteins; D008941:Spindle Apparatus
D000970:Antineoplastic Agents; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; C079620:SEC13 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D015739:Nocodazole