Solvent versatility of immobilized 3,5-dimethylphenylcarbamate of amylose in enantiomeric separations by HPLC.
CHIRALPAK IA is a new chiral stationary phase containing amylose 3.5-dimethylphenylcarbamate immobilized onto silica gel. It is compatible with the whole range of organic solvents. Its solvent versatility has been thoroughly investigated. The option to use a wide range of solvents, especially the "non-standards" ones, in the mobile phase enables the enhancement of chiral separation methods in terms of enantioselectivity, resolution, analysis time, sample injection and sample solubility. Parameters such as the mobile phase type, the nature of modifier and eluting strength of various solvents are examined and discussed. A guideline for method development and optimization on CHIRALPAK IA is also proposed.
Chiral Technologies Europe, Parc d'lnnovation, Bd. Gonthier d 'Andernach, 67404 Illkirch, Cedex, France. tzhang@chiral.fr
Tong
T
Zhang
Christophe
C
Kientzy
Pilar
P
Franco
Atsushi
A
Ohnishi
Yasuhiro
Y
Kagamiharb
Hidehiro
H
Kurosawa
D000688:Amylose / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D048448:Phenylcarbamates; D012997:Solvents / Q000737:chemistry*; D013237:Stereoisomerism
C504080:3,5-dimethylphenylcarbamate; D002219:Carbamates; D048448:Phenylcarbamates; D012997:Solvents; D000688:Amylose