Biochemical and molecular modeling analysis of the ability of two p-aminobenzamidine-based sorbents to selectively purify serine proteases (fibrinogenases) from snake venoms.
Snake venoms contain several trypsin-like enzymes with equivalent physicochemical characteristics and similar inhibition profiles. These are rather difficult to separate by classical purification procedures and therefore constitute a good model for affinity chromatography analysis. Some of these trypsin homologues present fibrinogenase activity, mimicking one or more features of the central mammalian coagulation enzyme, thrombin. It was previously demonstrated that a number of amidine derivatives are able to interact specifically with some of these serine proteases. To understand the enzyme-sorbent interactions we have investigated the ability of two commercially available benzamidine affinity matrices to purify thrombin-like serine proteases (TLSP) with similar biological properties from two snake venoms (Bothrops jararacussu and Lachesis muta rhombeata). Curiously, each sorbent retained a single but distinct TLSP from each venom with high yield. Molecular modeling analysis suggested that hydrophobic interactions within a specific region on the surface of these enzymes could be generated to explain this exquisite specificity. In addition, it was demonstrated that a specific tandem alignment of the two benzamidine sorbents enables the purification of three other enzymes from B. jararacussu venom.
Laboratório de Bioquímica de Proteínas e Peptídeos, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, 21045-900 Rio de Janeiro, RJ, Brazil. dsimone@ioc.fiocruz.br
S G
SG
De-Simone
C
C
Correa-Netto
O A C
OA
Antunes
R B
RB
De-Alencastro
F P
FP
Silva
D000595:Amino Acid Sequence; D000818:Animals; D001586:Benzoylarginine Nitroanilide / Q000737:chemistry*; D017837:Bothrops; D002846:Chromatography, Affinity / Q000379:methods*; D003435:Crotalid Venoms / Q000201:enzymology*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000737:chemistry; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae
C011568:4-aminobenzamidoethyl-sepharose; D003435:Crotalid Venoms; D015842:Serine Proteinase Inhibitors; D014757:Viper Venoms; D012685:Sepharose; D001586:Benzoylarginine Nitroanilide; D012697:Serine Endopeptidases