[Ecological interpretation and related strategies for low culturability of microorganisms].
Pure culture technique has been a fundamental method since its invention in microbiology, but its isolated and monotonous environment contradicts microbial diversity and cooperation relationship in natural environment. And this contradiction directly results in the uncultivability of some microbes in media. From ecological viewpoint, the recovery barriers of natural microbes mainly include the crash of cooperation in natural environment, the great change of nutrient in new environment and the loss of native niche. The main methods and strategies on low curturability of microorganisms are culture-independent methods, enrichment culture, mixed culture, dilution culture, simulating nature culture and synthetic method, which can to some degree compensate for the traditional approach and improve the low culturability of some bacteria.
Key Laboratory of Eco-environments of Three Gorges Reservoir Region of Ministry of Education, City Construction and Environmental Engineering Academy, Chongqing University, Chongqing 400045, China. yejy@swnu.edu.cn
Jiang-yu
JY
Ye
Gu-yuan
GY
Luo
D044822:Biodiversity; D003470:Culture Media; D017753:Ecosystem; D004783:Environmental Microbiology; D008828:Microbiological Techniques
D003470:Culture Media