Effects of total flavone of Abelmoschl Manihot L. Medic on the function of platelets and its mechanism.
OBJECTIVE
To study the effects of total flavone of Abelmoschl Manihot L. Medic (TFA) on the function of platelets and to explore its mechanism.


METHODS
Rat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca(2+)]i) was assayed by Fura-2 method.


RESULTS
TFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl(2)-induced increase of free intracellular calcium concentration ([Ca(2+)]i) in rabbit platelet in vitro.


CONCLUSIONS
TFA has an antiplatelet effect via the inhibition on the influx of Ca(2+).
Department of Pharmacology, Anhui Medical University, Hefei, China. guoyanzx@yahoo.com.cn
Yan
Y
Guo
Li
L
Fan
Liu-yi
LY
Dong
Zhi-wu
ZW
Chen
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002341:Carotid Artery Thrombosis / Q000097:blood* / Q000209:etiology; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D047309:Flavones / Q000008:administration & dosage / Q000494:pharmacology*; D006027:Glycosides / Q000008:administration & dosage / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism; D009994:Osmolar Concentration; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D010979:Platelet Function Tests; D011817:Rabbits / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D004365:Drugs, Chinese Herbal; D047309:Flavones; D006027:Glycosides; D010975:Platelet Aggregation Inhibitors; D003094:Collagen; D002122:Calcium Chloride; D002118:Calcium