Polymorphisms of interleukin-1 gene complex, IL6 and tumour necrosis factor genes in chronic idiopathic neutropenia of adults.
Chronic idiopathic neutropenia of adults (CINA) is a granulocytic disorder characterised by the "unexplained" decrease in the number of circulating neutrophils. Serum inflammatory cytokines and chemokines are increased in CINA. In addition, cytokines gene polymorphisms are associated with increased levels of respective products and related with inflammatory diseases. The aim of the present study was to investigate the association of polymorphisms of IL1B-511C/T and +3953C/T, IL1RN intron 2, IL6-174G/C and TNF-308G/A genes with CINA. We analysed 29 CINA and controls by polymerase chain reaction and restriction fragment length polymorphism. Statistical analyses were performed using chi2 test, and the Hardy-Weinberg equilibrium (HWE) was investigated. All alleles analysed were in HWE in both populations. Similar frequencies of IL1B-511C/T, IL1B+3953C/T, IL1RN, IL6-174G/C and TNF-308G/A genotypes were observed in CINA and controls. These results suggest that cytokine polymorphisms associated with control of gene expression and protein levels were not associated with occurrence of CINA and were not responsible for the increased cytokine in CINA patients.
Addas-Carvalho
Marcelo
M
Haematology and Hemotherapy Center, State University of Campinas, UNICAMP, Campinas, Sao Paulo, Brazil. maddas@unicamp.br
de Paula
Erich V
EV
Lima
Carmen S P
CS
Saad
Sara T O
ST
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009503:Neutropenia / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha