[The relationship between Helicobacter pylori infection and IL-8 expression in remnant stomach mucosa].
OBJECTIVE
To explore the relationship between helicobacter pylori (Hp) infection and expression of interleukin-8 (IL-8) in the remnant stomach mucosa.


METHODS
Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to detect Hp infection (by H.E. and Giemsa staining) and IL-8 expression (by real-time semi-quantitative RT-PCR).


RESULTS
Hp infection was detected in 65.5% (38/58)of the remnant stomach mucosa. In 34 patients with Billroth I (BI) anastomosis, IL-8 in corpus was significantly higher in Hp-positive patients than that in Hp-negative patients (0.11+/-0.07 vs 0.02+/-0.01, P<0.05). In 24 patients with Billroth II (BII) anastomosis, IL-8 in stoma and corpus in Hp-positive patients was significantly higher than that in Hp-negative patients (P<0.05).


CONCLUSIONS
Hp infection induces IL-8 expression in remnant stomach mucosa. In corpus, IL-8 mRNA expression is mainly related with Hp infection, while in stoma, IL-8 mRNA expressions may be related with bile reflux besides Hp infection.
Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Cheng-zhong
CZ
Xing
Xiao-lin
XL
Guo
Hui-mian
HM
Xu
Yuan
Y
Yuan
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020776:Endoscopes, Gastrointestinal; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000601:surgery; D018530:Gastric Stump; D005763:Gastroenterostomy; D005786:Gene Expression Regulation; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology; D016480:Helicobacter pylori; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D016209:Interleukin-8; D012333:RNA, Messenger