Studies on the spectral-luminescent properties of the novel homodimer styryl dyes in complexes with DNA.
Series of homodimer styryls containing on (p-dimethylaminostyryl) pyridinium residues that are connected with aliphatic linkage group was synthesized. Spectral luminescent properties of obtained dyes in free state and in nucleic acids presence were studied. It was shown that DNA binding affinity of the novel homodimers exceeds that of parent monomer (p-dimethylaminostyryl)pyridine iodide. For homodimers with the linkage 4-10 carbon atoms preference in binding to DNA than to RNA was observed. It could be concluded that parent monomer has different mechanisms of binding to nucleic acids than corresponding homodimer dye.
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, 03143 Kyiv, Ukraine.
V B
VB
Kovalska
I O
IO
Kocheshev
D V
DV
Kryvorotenko
A
A
Balanda
S M
SM
Yarmoluk
D000818:Animals; D002645:Chickens; D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D066298:In Vitro Techniques; D049449:Luminescence; D046911:Macromolecular Substances; D015394:Molecular Structure; D012331:RNA, Fungal / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013343:Styrenes / Q000138:chemical synthesis / Q000737:chemistry
D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D012331:RNA, Fungal; D013343:Styrenes; D004247:DNA