Supercritical fluid extraction of sinomenine from Sinomenium acutum (Thumb) Rehd et Wils.
Supercritical carbon dioxide, with and without a methanol modifier, was used to extract sinomenine from Sinomenium acutum (Thumb) Rehd et Wils. Sinomenine determinations were carried out using high-performance liquid chromatography (HPLC). The results show that the yield obtained after 2.5 h extraction with methanol-modified supercritical carbon dioxide was the highest (7.47 mg/g), while that obtained with only supercritical carbon dioxide was the lowest (0.17 mg/g). The recovery obtained with supercritical carbon dioxide, with and without a methanol modifier, could not be increased greatly by the method of the alkalinization of sample. Higher recoveries were obtained than extraction using methanol in Soxhlet extractor.
Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. liu_ben0@hotmail.com
Ben
B
Liu
Hailiang
H
Jiang
Bo
B
Shen
Yiling
Y
Chang
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D009019:Morphinans / Q000302:isolation & purification*; D032841:Sinomenium / Q000737:chemistry*
D009019:Morphinans; C009271:sinomenine