Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections.
A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.
Morris
Julie M
JM
Arctic Investigations Program, CDC, 4055 Tudor Centre Drive, Anchorage, AK 99508, USA. jmorris@cdc.gov
Reasonover
Alisa L
AL
Bruce
Michael G
MG
Bruden
Dana L
DL
McMahon
Brian J
BJ
Sacco
Frank D
FD
Berg
Douglas E
DE
Parkinson
Alan J
AJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D005456:Fluorescent Dyes; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008826:Microbial Sensitivity Tests; D016612:Paraffin Embedding; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013270:Stomach; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; D017291:Clarithromycin