The adrenocortical tumor cell line NCI-H295R as an in vitro screening system for the evaluation of CYP11B2 (aldosterone synthase) and CYP11B1 (steroid-11beta-hydroxylase) inhibitors.
Aldosterone plays a key role in salt and water homeostasis but is also involved in the development and progression of congestive heart failure and myocardial fibrosis. As a new pharmacological strategy for the treatment of these diseases, we propose the inhibition of the key enzyme of mineralcorticoid formation, CYP11B2 (aldosterone synthase). For studies of the effects of CYP11B2 inhibitors on the adrenal cortex, we selected the NCI-H295R cell line which expresses most of the key enzymes necessary for steroidogenesis. To evaluate this cell line as a test system for effects and side effects of CYP inhibitors, we established assays using radiolabeled substrates of CYP11B2 and CYP11B1 and subsequently tested a series of CYP11B2 inhibitors including the CYP19 inhibitor fadrozole. Fadrozole and compounds 6, 9 and 10 were more potent towards CYP11B2 compared to CYP11B1 with IC(50) values in the nanomolar range. To analyze their overall effect, the formation of steroids in the cell culture supernatant was monitored. All compounds led to a concentration-dependent reduction of the aldosterone secretion but also reduced the formation of cortisol and androgens. In conclusion, the H295R cell line is a suitable tool for the prediction of overall side effects of CYP11B2 inhibitors on steroidogenesis.
Pharmaceutical and Medicinal Chemistry, Saarland University, Saarbrücken D-66041, Germany.
Ursula
U
Müller-Vieira
Marc
M
Angotti
Rolf W
RW
Hartmann
D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D000450:Aldosterone / Q000378:metabolism; D047072:Aromatase Inhibitors / Q000494:pharmacology; D045744:Cell Line, Tumor; D019405:Cytochrome P-450 CYP11B2 / Q000037:antagonists & inhibitors*; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D017316:Fadrozole / Q000494:pharmacology; D006801:Humans; D013252:Steroid 11-beta-Hydroxylase / Q000037:antagonists & inhibitors*; D013256:Steroids / Q000032:analysis / Q000378:metabolism
D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors; D013256:Steroids; D000450:Aldosterone; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase; D017316:Fadrozole