Molecular characterisation of Xanthomonas strains isolated from aroids in Mauritius.
Mauritius is one of the largest world producers of Anthurium cut flowers but outbreaks of bacterial blight have never been reported on the island. This work was about the characterisation and identification of bacterial strains isolated from Anthurium andreanum, Dieffenbachia maculata and Aglaonema simplex in Mauritius. Fifteen strains, that showed the morphological properties of Xanthomonas on conventional media, were tested on two semi-selective media (Esculin-trehalose and cellobiose-starch). ELISA tests using a panel of monoclonal antibodies were carried out and three out of 15 strains reacted with a Xanthomonas-specific monoclonal antibody (MAb XII). Analysis using four sets of ribosomal primers revealed that the same three Mauritius strains shared conserved PCR products with reference xanthomonads including virulent strains of Xanthomonas axonopodis pv. dieffenbachiae (Xad). BIOLOG tests and the Sherlock Microbial Identification system (MIDI) identified these three new strains at the species level as X. axonopodis. The complementary tests that were carried out clearly confirmed that the three strains are xanthomonads and, moreover, a DNA probe which showed specificity to Xad strains suggested that the three Mauritius strains are non-virulent forms of the pathogen causing Anthurium blight.
Biotechnology Unit, Faculty of Agriculture, University of Mauritius, Mauritius.
M H R
MH
Khoodoo
F
F
Sahin
M F
MF
Donmez
Y Jaufeerally
YJ
Fakim
D000942:Antigens, Bacterial / Q000032:analysis; D029064:Araceae / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000032:analysis; D008436:Mauritius; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D005227:Fatty Acids