[Evaluation on the biosafety of classical swine fever DNA vaccine].
The biosafety of DNA vaccine is one of the key questions which should be solved before it is used in the clinical trail. In order to evaluate the biosafety of DNA vaccine, the CSFV DNA vaccine was used in the studying target, two main aspects of the vaccine were explored in the study. Firstly, the possibility of integration of two kinds of DNA vaccine plasmids into pig genome was analyzed by PCR technology after the different vaccines were injected through the intramuscular introduction. The results showed that both plasmids DNA were detected as the form were not integrated into pig genome, it can be detected 30 copies plasmid DNA in 1 microg total genomic DNA as the sensibility of PCR, indicated the safety of the DNA vaccine. Afterward the environmental fecal and soil samples in the experimental pens were picked up. Then the antibiotic resistant bacteria were isolated and its resistant genes were analyzed by PCR and gene sequencing. The results demonstrated that the transfer and spreading of two DNA vaccine plasmids studied into environmental bacteria from receptor pigs were not found. The results showed that the CSFV DNA vaccine is safe to both pigs and the surrounding environment.
Military Veterinary Research Institute of the Academy of Military Medical sciences, Changchun 130062, China. csqu2001@hotmail.com
Cong-sheng
CS
Cheng
Wen-cheng
WC
Wang
Su
S
Li
Rong-liang
RL
Hu
Chang-chun
CC
Tu
D000818:Animals; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D016679:Genome, Viral; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012449:Safety; D013552:Swine; D019444:Vaccines, DNA / Q000009:adverse effects* / Q000235:genetics; D014765:Viral Vaccines / Q000009:adverse effects* / Q000235:genetics
D019444:Vaccines, DNA; D014765:Viral Vaccines