[Study on apoptosis of human cervical carcinoma HeLa cells with RNA interference targeting hTERT].
OBJECTIVE
To study the induction of tumor cell apoptosis by RNA interference-mediated inhibition of the expression of telomerase in cancer cells.


METHODS
HeLa cells were transfected with the successfully established siRNA(small interfering RNA) expression vectors targeting hTERT (human telomerase reverse transcriptase). By electronic microscopy, Western blot and FCM (flow cytometry), the apoptosis of HeLa cells was tested.


RESULTS
The established siRNA expression vectors could induce apoptosis of HeLa cells.


CONCLUSIONS
Transfection of siRNA expression vectors targeting hTERT can induce apoptosis of HeLa cells.
Zhao
Ying
Y
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. zhaoying@fmmu.edu.cn
Ren
Jun-lin
JL
Zhang
Rui
R
Ma
Long-yang
LY
Bao
Wei
W
Wang
Cheng-ji
CJ
Yang
An-gang
AG
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D034622:RNA Interference; D019098:Telomerase / Q000235:genetics*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
D019098:Telomerase