[Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma].
OBJECTIVE
To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).


METHODS
Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).


RESULTS
The expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01).


CONCLUSIONS
The expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Li-jie
LJ
Song
Sheng-long
SL
Ye
Kai-feng
KF
Wang
Yong-qiang
YQ
Weng
Chun-min
CM
Liang
Rui-xia
RX
Sun
Yan
Y
Zhao
Yin-kun
YK
Liu
Zhao-you
ZY
Tang
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D020690:GTPase-Activating Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016297:Mutagenesis, Site-Directed; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
C112352:DLC1 protein, human; D020690:GTPase-Activating Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins