Development of a novel protein microarray method for serotyping Salmonella enterica strains.
An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allowed complete serovar identification of 86 target strains and partial identification of 30 target strains and allowed exclusion of the 73 nontarget strains from the target serovars.
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Laboratory Services Division, University of Guelph, P.O. Box 3650, 95 Stone Road West, Guelph, Ontario, Canada N1H 8J7.
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Muckle
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Prescott
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Chen
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D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis; D015373:Bacterial Typing Techniques; D005407:Flagella / Q000276:immunology; D006801:Humans; D019081:O Antigens / Q000032:analysis; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D019779:Salmonella enterica / Q000145:classification*; D012703:Serotyping
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D019081:O Antigens