Amyloid-beta oligomers are inefficiently measured by enzyme-linked immunosorbent assay.
Amyloid-beta (Abeta) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Abeta results in underestimated Abeta ELISA levels. The implications are that comprehensive analysis of soluble Abeta requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.
Department of Public Health/Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
Charlotte
C
Stenh
Hillevi
H
Englund
Anna
A
Lord
Ann-Sofi
AS
Johansson
Claudia G
CG
Almeida
Pär
P
Gellerfors
Paul
P
Greengard
Gunnar K
GK
Gouras
Lars
L
Lannfelt
Lars N G
LN
Nilsson
D000544:Alzheimer Disease / Q000175:diagnosis; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D014162:Transfection
D016229:Amyloid beta-Peptides