Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1.
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.
Department of Biological Science, School of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California 92834-6850, USA.
Atousa
A
Pourreza
Mavee
M
Witherspoon
Jessica
J
Fox
Jason
J
Newmark
Duyen
D
Bui
Marcelo E
ME
Tolmasky
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000409:Alanine; D000583:Amikacin / Q000494:pharmacology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007612:Kanamycin; D000105:Acetyl Coenzyme A; D000583:Amikacin; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase; D000409:Alanine