Restriction mapping in nanofluidic devices.
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of approximately 1.5 kbp in 1 min using single DNA molecules.
Riehn
Robert
R
Departments of Physics, Princeton University, Princeton, NJ 08544, USA. rha@suiling.princeton.edu
Lu
Manchun
M
Wang
Yan-Mei
YM
Lim
Shuang Fang
SF
Cox
Edward C
EC
Austin
Robert H
RH
D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D008274:Magnesium; D008853:Microscopy; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods*
D004247:DNA; D004262:DNA Restriction Enzymes; D008274:Magnesium