Effects of a nicotine conjugate vaccine on the acquisition and maintenance of nicotine self-administration in rats.
BACKGROUND
Immunization of rats against nicotine using a nicotine conjugate vaccine reduces the distribution of nicotine to brain in rats and attenuates some of nicotine's physiological and behavioral effects. It is not known whether such a vaccine can attenuate nicotine's reinforcing effects.


OBJECTIVE
The present experiment was conducted to determine whether a nicotine conjugate vaccine could interfere with the acquisition and maintenance of nicotine self-administration (NSA) in rats given 23 h day(-1) access to nicotine.


METHODS
To examine acquisition of NSA, rats were vaccinated with nicotine or control immunogen prior to being given access to a 0.01 mg kg(-1) infusion(-1) nicotine under a fixed-ratio(FR) 1 schedule for week 1, FR 2 for week 2, and FR 3 for week 3. Acquisition of cocaine self-administration (CSA) was similarly examined to determine the specificity of vaccination effects. To examine maintenance of NSA, rats were initially trained to self-administer nicotine under an FR 3 schedule, and then vaccinated with nicotine or control immunogen while NSA continued to be monitored.


RESULTS
NSA was significantly lower in vaccinated rats compared to controls during the acquisition protocol, with a 38% decrease in the number of infusions during the last week of training. The percentage of rats meeting acquisition criteria in the vaccinated group was lower (36%) than that in the control group (70%), but this difference was not statistically significant. Vaccination did not affect acquisition of CSA, demonstrating its specificity for nicotine. Maintenance of NSA was significantly reduced in vaccinated rats as compared to controls after the final vaccine injection, with a mean reduction of 57%. There was no evidence in either protocol that vaccinated rats attempted to compensate for altered nicotine distribution by increasing nicotine intake.


CONCLUSIONS
These data suggest that vaccination against nicotine can reduce the reinforcing effects of nicotine in rats and may have therapeutic potential for the treatment of tobacco dependence.
LeSage
Mark G
MG
Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA. mlesage@mmrf.org
Keyler
Daniel E
DE
Hieda
Yoko
Y
Collins
Greg
G
Burroughs
Danielle
D
Le
Chap
C
Pentel
Paul R
PR
D000818:Animals; D003216:Conditioning, Operant; D004195:Disease Models, Animal; D007117:Immunization, Secondary; D008297:Male; D009538:Nicotine / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012055:Reinforcement Schedule; D012646:Self Administration; D014018:Tissue Distribution; D014029:Tobacco Use Disorder / Q000276:immunology* / Q000517:prevention & control; D018074:Vaccines, Conjugate / Q000276:immunology / Q000494:pharmacology*
D018074:Vaccines, Conjugate; D009538:Nicotine