Optimization of the sonication extraction method of Hibiscus tiliaceus L. flowers.
The influence of several experimental parameters on the ultrasonic extraction of Hibiscus tiliaceus L. flowers were investigated: extraction time, solvent polarity, sample amount, solvent volume and sample particle size. It was concluded that the most influential variables were extraction time and solvent polarity. The optimized procedure employed 5 g of ground flowers, 150 mL of methanol and 140 min of extraction. The extracts were fractionated using preparative silica columns and the resulting fractions were analyzed by GC/MS. Some saturated hydrocarbons, fatty acids, fatty acid methyl esters, phytosterols, and vitamin E were identified in the plant extracts.
Chemistry Institute, UFRGS, Porto Alegre, RS, av. Bento Gonçalves, 9500, CEP 91501-960, RS, Brazil.
Maria Inês Soares
MI
Melecchi
Valéria Flores
VF
Péres
Cláudio
C
Dariva
Claudia Alcaraz
CA
Zini
Fernanda Contieri
FC
Abad
Migdália Miranda
MM
Martinez
Elina Bastos
EB
Caramão
D035264:Flowers / Q000737:chemistry; D031584:Hibiscus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013010:Sonication
D010936:Plant Extracts; D012997:Solvents