The synthetic peptide Trp-Lys-Tyr-Met-Val-D-Met inhibits human monocyte-derived dendritic cell maturation via formyl peptide receptor and formyl peptide receptor-like 2.
Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm) has been reported to stimulate monocytes, neutrophils, and dendritic cells (DCs). However, although WKYMVm has been reported to function as a DC chemoattractant, its role on DC maturation has not been examined. In this study, we investigated the effects of WKYMVm on human DC maturation. The costimulation of DCs with WKYMVm and LPS dramatically inhibited LPS-induced IL-12 production, CD86 and HLA-DR surface expression, and DC-mediated T cell proliferation. However, DC phagocytic activity was increased by WKYMVm stimulation. These findings demonstrate that WKYMVm inhibits DC maturation by LPS. In terms of the mechanism underlying DC maturation inhibition by WKYMVm, we found that LPS-induced DC maturation was negatively regulated by WKYMVm-stimulated ERK activity. Moreover, the costimulation of DCs with WKYMVm and LPS dramatically inhibited the LPS-induced accumulations of IL-12 mRNA, thus suggesting that WKYMVm inhibits LPS-induced IL-12 production at the transcriptional level. We also found that DCs express two WKYMVm receptors, formyl peptide receptor (FPR) and FPR-like 2 (FPRL2). In addition, formyl-Met-Leu-Phe (a FPR ligand), Trp-Lys-Tyr-Met-Val-Met, Hp(2-20) peptide, and F2L (three FPRL2 ligands) inhibited LPS-induced IL-12 production in DCs. Taken together, our findings indicate that the activations of FPR and FPRL2 inhibit LPS-induced DC maturation, and suggest that these two receptors should be regarded as important potential therapeutic targets for the modulation of DC maturation.
Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Korea.
Hyun Kyu
HK
Kang
Ha-Young
HY
Lee
Mi-Kyoung
MK
Kim
Kyoung Sun
KS
Park
Yeong Min
YM
Park
Jong-Young
JY
Kwak
Yoe-Sik
YS
Bae
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000502:physiology; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D004789:Enzyme Activation / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism / Q000502:physiology; D006131:Growth Inhibitors / Q000138:chemical synthesis / Q000502:physiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000502:physiology*; D010446:Peptide Fragments / Q000502:physiology; D010587:Phagocytosis / Q000276:immunology; D044042:Receptors, Formyl Peptide / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology
D001426:Bacterial Proteins; C469985:FPR3 protein, human; D006131:Growth Inhibitors; C433783:Hp (2-20) protein, Helicobacter pylori; D008070:Lipopolysaccharides; D009842:Oligopeptides; D010446:Peptide Fragments; D044042:Receptors, Formyl Peptide; C113617:Trp-Lys-Tyr-Met-Val-Met; D018664:Interleukin-12; D048049:Extracellular Signal-Regulated MAP Kinases