The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin.
The gene for the protein moiety of the light-driven chloride pump halorhodopsin (HR), hop gene, was sequenced and the primary structure of the protein derived thereof. The gene has a GC content of 67% and codes for 274 amino acids. A promoter structure, resembling that of the halobacterial 16S rRNA genes, is present and both a terminating stem and a loop sequence is found downstream of the TGA stop codon. A ribosomal binding site is located within the translated region. The HR protein moiety is processed at the amino terminus, as well as the carboxy terminus, yielding a dominant species of calculated Mr 26 961.
Max-Planck Institut für Biochemie, D-8033, Martinsried, FRG.
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Blanck
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Oesterhelt
D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D025602:Halorhodopsins / Q000235:genetics*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D015183:Restriction Mapping
D025602:Halorhodopsins; D001436:Bacteriorhodopsins