Gene expression profiling reveals the mechanism of action of anticonvulsant drug QYS.
We have examined the gene expression profiling of inferior colliculus from DBA/2J mice with high-intensity noise induced audiogenic seizure (AGS). We have also tested the effects of Qingyangshenylycosides (QYS), a traditional Chinese medicine, on the audiogenic seizure, and examined how the drug affected the gene expressions in inferior colliculus. Our results demonstrated that the latency was increased and the Tonus% of AGS was decreased in the animals treated with QYS, indicating that the drug effectively prevented audiogenic seizure. Gene expression analysis using Agilent oligo microarray showed that total of 134 genes were either up- or down-regulated during AGS. QYS prevented many of the AGS induced gene expression changes. Nevertheless, some of the AGS induced genes were further enhanced or reversed by QYS treatment. Our gene expression profiling data provided important information regarding the molecular mechanisms of AGS and the mechanism of action of QYS. Further analysis of the function of these genes may help to identify therapeutic targets for epilepsy.
Key Lab of Brain Functional Genomics, MOE&STCSM, Shanghai Institute of Brain Functional Genomics, East China Normal University, 3663 Zhongshan Road N., Shanghai 200062, China.
Xianchun
X
Li
Qingxiong
Q
Yang
Yinghe
Y
Hu
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020195:Epilepsy, Reflex / Q000188:drug therapy / Q000209:etiology; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D008811:Mice, Inbred DBA; D046228:Microarray Analysis / Q000379:methods; D009622:Noise / Q000009:adverse effects; D012333:RNA, Messenger / Q000096:biosynthesis; D011930:Reaction Time / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D000927:Anticonvulsants; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger