Eph/ephrin A- and B-family expression patterns in the leopard frog (Rana utricularia).
Eph/ephrin expression was studied in Rana utricularia larvae and adults with in situ receptor and ligand affinity probes. From stages TK-II (early limb bud) to VI (early foot paddle larva), tectal EphB expression is highest in a band extending transversely across the posterior optic tectum and grades off anteriorly and posteriorly. The ephrin-A expression gradient is parallel to the EphB gradient rather than being orthogonal to it. However, its high point occupies the posterior pole, and it runs from high-posteriorly to low-anteriorly. Tectal EphA expression is high anteriorly and low posteriorly, while ephrin-Bs are expressed only in a thin line at the dorsal midline. At later stages and in adults, tectal EphB expression becomes uniform.
Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA. fscalia@downstate.edu
Frank
F
Scalia
David A
DA
Feldheim
D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002460:Cell Line; D036342:Ephrins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D047468:Immunoprecipitation / Q000379:methods; D007814:Larva; D008024:Ligands; D051379:Mice; D011485:Protein Binding; D011894:Rana pipiens / Q000033:anatomy & histology / Q000502:physiology*; D036081:Receptors, Eph Family / Q000145:classification / Q000378:metabolism; D013477:Superior Colliculi / Q000254:growth & development / Q000378:metabolism; D014162:Transfection / Q000379:methods; D014795:Visual Pathways / Q000254:growth & development / Q000378:metabolism
D036342:Ephrins; D008024:Ligands; D036081:Receptors, Eph Family