Tissue processing prior to protein analysis and amyloid-beta quantitation.
Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well-suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate, multiple characterizations can be done, including Western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Abeta in the brain of a transgenic mouse model of beta-amyloid deposition. The ELISA technique is described in detail in the following chapter.
Schmidt
Stephen D
SD
Center for Dementia Research, Nathan Kline Institute, Orangeburg, NY, USA.
Jiang
Ying
Y
Nixon
Ralph A
RA
Mathews
Paul M
PM
D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000378:metabolism; D006801:Humans; D051379:Mice; D016707:Tissue Fixation / Q000379:methods
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor