[The role of oxidative metabolism disturbance in the development of NO-related endothelial dysfunction during chronic hearth failure].
The aim of the work was to establish the oxidative metabolism changes and NO data in Chronic Hearth Failure (HF). 52 patients were included in the investigation, among them 37 patients with CHD and chronic HF (II-IV functional class by NIHA) and 17 without it (control group). For revealing of organism redox-status (ceruloplasmine, Fe3+-transfferine, Mn2+, methemoglobine) the blood paramagnetic centers was studied by electron paramagnetic resonance method. For revealing of blood free NO, the diethyldithiocarbamat (SIGMA) was used. In chronic HF the oxidative process intensification and organism compensate reaction reduction with low Fe3+-transferine levels, increased Mn2++, methaemoglobin and inactivation of erythrocytes membranes adrenergic receptors were revealed. In chronic HF the accumulation of reactive oxygen levels provoke NO transformation in peroxynitrote with following decreases of blood free NO and develop the endothelial dysfunction.
A.Natishvili Institute of Experimental Morphology, Academy of Sciences of Georgia.
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N
Goishvili
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Kakauridze
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Sanikidze
D002303:Cardiac Output, Low / Q000209:etiology / Q000378:metabolism*; D002570:Ceruloplasmin / Q000032:analysis; D002908:Chronic Disease; D004578:Electron Spin Resonance Spectroscopy; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008706:Methemoglobin / Q000032:analysis; D017202:Myocardial Ischemia / Q000150:complications*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D014168:Transferrin / Q000032:analysis
D014168:Transferrin; D009569:Nitric Oxide; D008706:Methemoglobin; D002570:Ceruloplasmin