Adhesiveness of beta5 integrin variant lacking FNK767-769 is similar to that of the prototype containing FNKFNK764-769.
Little is known about the functions of two different beta5 integrins: repeated-FNK (FNKFNK764-769) and single-FNK (FNK764-766) amino acid sequences in the cytoplasmic domain. We examined whether they occurred as germ line mutations or somatic mutations associated with neoplastic transformation, and whether there were functional alterations. Out of six cultured cell lines, only KATO-III cells had the single-FNK beta5 sequence. The single-FNK beta5 was found in 9 out of 79 patients with colon carcinoma, but no somatic mutations were detected in cancerous tissues. CHO cells were transformed with expression vectors containing single-FNK or repeated-FNK beta5 cDNA, which were derived from KATO-III cells. CHO cells transfected with single-FNK and repeated-FNK showed similar adhesiveness to, and proliferative activity on, vitronectin substrates.
Kawahara
Ei
E
Department of Laboratory Sciences, School of Health Sciences, Kanazawa University Faculty of Medicine, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan. kawahara@kenroku.kanazawa-u.ac.jp
Saito
Ayumi
A
Kobayashi
Jun
J
Maenaka
Shiori
S
Minamoto
Toshinari
T
Imai
Miwa Akasofu
MA
Oda
Yoshio
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms; D006224:Cricetinae; D003412:Cricetulus; D014644:Genetic Variation; D018095:Germ-Line Mutation; D006801:Humans; D039641:Integrin beta Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
D039641:Integrin beta Chains; D008565:Membrane Proteins; D012333:RNA, Messenger; C084344:integrin beta5