Poly(ADP-ribose)polymerase-1 is required for integration of the human immunodeficiency virus type 1 genome near centromeric alphoid DNA in human and murine cells.
This study examined the efficiency of human immunodeficiency virus type 1 (HIV-1) integration in poly(ADP-ribose)polymerase-1 (PARP-1)-deficient murine cells and in human cell lines transfected with small interfering RNA against PARP-1 (PARP-1 siRNA). To semi-quantify the amount of integrated HIV-1 genome, real-time nested PCR was carried out using primers specific for Alu and alphoid DNA combined with primers for the HIV-1 genome. The results showed that the integration efficiency of the HIV-1 genome near Alu DNA, which is randomly distributed in the chromosome, is reduced in PARP-1-deficient murine cells, but not in PARP-1 siRNA-transfected human cells. By contrast, the integration efficiency of the HIV-1 genome near alphoid DNA, which is localized in the centromere region, is significantly reduced in PARP-1-deficient murine cells and in PARP-1 siRNA-transfected human cells. These results suggest that PARP-1 is required for HIV-1 integration near the centromere region both in human and murine cells.
Department of Biochemistry, Nara Medical University School of Medicine, Kashihara, Nara 634-8521, Japan. mkameoka@biken.osaka-u.ac.jp
Masanori
M
Kameoka
Souichi
S
Nukuzuma
Asako
A
Itaya
Yasuharu
Y
Tanaka
Katsuya
K
Ota
Yoshiaki
Y
Inada
Kazuyoshi
K
Ikuta
Koichiro
K
Yoshihara
D000818:Animals; D002460:Cell Line; D002503:Centromere / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D016679:Genome, Viral; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000172:deficiency*; D016662:Virus Integration / Q000502:physiology*
D004247:DNA; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C459820:Parp2 protein, mouse