Cloning and characterization of the Cu,Zn superoxide dismutase (SOD1) cDNA from the mole cricket, Gryllotalpa orientalis.
A Cu,Zn superoxide dismutase (SOD1) cDNA was cloned from the mole cricket, Gryllotalpa orientalis. The G. orientalis SOD1 (GoSOD1) cDNA contains an open reading frame of 462 bp encoding 154 amino acid polypeptide with a predicted molecular mass of 15.8 kDa and pI of 6.1, and possesses the typical metal binding ligands of six histidines and one aspartic acid common to SOD1s. The deduced amino acid sequence of the GoSOD1 cDNA showed 75% identity to Lasius niger SOD1, 73% to Apis mellifera SOD1, and 70-68% to SOD1 sequences from other insects. Northern blot analysis revealed the presence of GoSOD1 transcripts in all tissues examined. The expression level of GoATX1 mRNA in the fat body was induced when G. orientalis adult was exposed at low (4 degrees C) and high (37 degrees C) temperatures, suggesting that the GoSOD1 seems to play a protective role against oxidative stress caused by temperature shock.
Kim
Iksoo
I
Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon, 441-100, Korea.
Lee
Kwang Sik
KS
Choi
Young Soo
YS
Hwang
Jae Sam
JS
Sohn
Hung Dae
HD
Jin
Byung Rae
BR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D006135:Gryllidae / Q000201:enzymology / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics*; D013696:Temperature
D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D013482:Superoxide Dismutase