Detection of prenatal exposure to several classes of environmental toxicants and their metabolites by gas chromatography-mass spectrometry in maternal and umbilical cord blood.
We developed a sensitive method to detect several classes of pesticides and their metabolites in maternal and cord whole blood using electron-impact gas chromatography-mass spectrometry (GC-MS). The method can detect parent and metabolite compounds at levels of <0.10 and 0.20mug/mL, respectively, with high accuracy and recovery. Analysis of blood from mother-infant dyads from an area of high pesticide use in the Philippines showed detectable levels of propoxur, 3-phenoxybenzoic acid (3-PBA), and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE) in maternal and umbilical cord blood. GC-MS analysis of several classes of parent pesticides and their metabolites in maternal and cord blood provides a sensitive and specific method to detect pesticide exposure during pregnancy.
Department of Pediatrics, Hutzel Women's Hospital, Children's Hospital of Michigan, Wayne State University School of Medicine, 4707 St. Antoine, Detroit, MI 48201, USA.
M L
ML
Corrion
E M
EM
Ostrea
D M
DM
Bielawski
N C
NC
Posecion
J J
JJ
Seagraves
D001565:Benzoates / Q000097:blood; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D004785:Environmental Pollutants / Q000032:analysis* / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D010575:Pesticides / Q000097:blood*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D001074:Propoxur / Q000097:blood
D001565:Benzoates; D004785:Environmental Pollutants; D010575:Pesticides; D003633:Dichlorodiphenyl Dichloroethylene; C017618:3-phenoxybenzoic acid; D001074:Propoxur