Enzyme nanoparticles-based electronic biosensor.
A simple and effective method to prepare an enzyme electronic biosensor by immobilizing enzyme nanoparticles directly onto the gold electrode surface is described; prepared horseradish peroxidase nanoparticles have been successfully used to develop reagentless electronic biosensors for H2O2 detection without promoters and mediators and offer great potential to develop enzyme-based electronic biosensors.
Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Guodong
G
Liu
Yuehe
Y
Lin
Veronika
V
Ostatná
Joseph
J
Wang
D015374:Biosensing Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013056:Spectrophotometry, Ultraviolet
D004800:Enzymes, Immobilized; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase