RanBPM is an L1-interacting protein that regulates L1-mediated mitogen-activated protein kinase activation.
A yeast two-hybrid screen using the last 28 amino acids of the cytoplasmic domain of the neural cell adhesion molecule L1 identified RanBPM as an L1-interacting protein. RanBPM associates with L1 in vivo and the N-terminal region of RanBPM (N-RanBPM), containing the SPRY domain, is sufficient for the interaction with L1 in a glutathione S-transferase pull-down assay. L1 antibody patching dramatically changes the subcellular localization of N-RanBPM in transfected COS cells. Overexpression of N-RanBPM in COS cells reduces L1-triggered extracellular signal-regulated kinase 1/2 activation by 50% and overexpression of N-RanBPM in primary neurons inhibits L1-mediated neurite outgrowth and branching. These data suggest that RanBPM is an adaptor protein that links L1 to the extracellular signal-regulated kinase/MAPK pathway.
Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio, USA.
Ling
L
Cheng
Sandra
S
Lemmon
Vance
V
Lemmon
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins; D004347:Drug Interactions; D004789:Enzyme Activation / Q000502:physiology; D039841:Leukocyte L1 Antigen Complex / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016501:Neurites / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D020931:ran GTP-Binding Protein / Q000235:genetics / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D039841:Leukocyte L1 Antigen Complex; D009687:Nuclear Proteins; C116005:Ran binding protein 9; D020928:Mitogen-Activated Protein Kinases; D020931:ran GTP-Binding Protein