Identification of microsatellite DNA markers for the giant anteater Myrmecophaga tridactyla.
The giant anteater (Myrmecophaga tridactyla) is found from Belize and Guatemala to Paraguay and Argentina. Its conservation status is considered vulnerable by IUCN. Here we report the isolation and characterization of six microsatellite loci. Positive loci for (GT)(n) were isolated using a magnetic bead hybridization selection protocol. The number of alleles per locus as well as the heterozygosity and PCR conditions are described. These loci will be useful for studying population structure, genetic diversity, and paternity in M. tridactyla wild populations.
Garcia
J E
JE
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil. jegarcia@uel.br
Vilas Boas
L A
LA
Lemos
M V F
MV
de Macedo Lemos
E G
EG
Contel
E P B
EP
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D004490:Xenarthra / Q000235:genetics*
D017931:DNA Primers