[Prokaryotic expression of HPV 16 L1 gene and identification of its immune activity].
OBJECTIVE
To express the major capsid protein of human papillomavirus type 16 L1(HPV 16 L1) in E.coli and identify its immune activity.


METHODS
The L1 gene of HPV 16 was cloned into the expression vector pThioHisC. The recombinant expression vector was transformed into E.coli, and the HisC-L1 protein was expressed under IPTG induction. The fusion protein was characterized by SDS-PAGE and Western blot.


RESULTS
The HPV16-L1 gene in plasmid pThioHisC was expressed in E.coli as a fusion protein with M(r) about 70,800. The fusion protein reacted specifically with antibodies against HPV16-L1.


CONCLUSIONS
The HPV-16 L1 gene was expressed successfully in E.coli, which provides necessary basis for preparing HPV-16 L1 vaccine in human.
Hao
Dong-feng
DF
Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China.
Ma
Zheng-hai
ZH
Wang
Yan-ping
YP
Rexidan
Zhang
Fu-chun
FC
D000918:Antibody Specificity; D015153:Blotting, Western; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; C063317:L1 protein, Human papillomavirus type 16; D004262:DNA Restriction Enzymes