Free radical scavenging and hepatoprotective constituents from the leaves of Juglans sinensis.
In the course of searching for hepatoprotective agents from natural products, six compounds were isolated from the MeOH extract of the leaves of Juglans sinensis, as guided by their DPPH free radical scavenging activity. The structures were determined as juglanoside B (1), quercetin 3-O-alpha-L-arabinofuranoside (avicularin, 2), quercetin 3-O-alpha-L-arabinopyranoside (guaijaverin, 3), quercetin 3-O-alpha-L-rhamnopyranoside (quercitrin, 4), (+)-catechin (5) and quercetin 3-O-beta-D-galactopyranoside (hyperin, 6). Compounds 2-6 showed significant DPPH free radical scavenging effects. An evaluation for the hepatoprotective activity of the isolated compounds on drug-induced cytotoxicity was conducted, and compounds 1, 2, and 5 showed protective effects against nitrofurantoin-induced cytotoxicity, and compound 5 also exhibited a moderate protective effect on amiodarone-induced cytotoxicity in Hep G2 cells.
College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
Ren-Bo
RB
An
Hyun-Chul
HC
Kim
Yu-Hua
YH
Tian
Youn-Chul
YC
Kim
D002460:Cell Line; D016166:Free Radical Scavengers / Q000302:isolation & purification*; D006801:Humans; D031324:Juglans / Q000737:chemistry*; D008099:Liver / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry*; D020011:Protective Agents / Q000302:isolation & purification
D016166:Free Radical Scavengers; D020011:Protective Agents