[The study on the relationship between the tumor-like proliferation of synoviocytes and the expression of c-myc and ODC genes in adjuvant arthritis rats].
OBJECTIVE
To investigate the mechanism of the tumor-like proliferation of synoviocytes in adjuvant arthritis (AA) rats.


METHODS
Wistar rats were randomly divided into 2 groups, AA group and control group. Freund's complete adjuvant was injected into AA group rats to induce adjuvant arthritis. The knee synovial tissues of the rats were taken and synoviocytes were separated and cultured in vitro. The proliferation of cultured synoviocytes was measured by MTT colorimetry. Meanwhile, the mRNAs of C-myc and ODC genes in synovial tissues of the rats were detected by semi-quantitative RT-PCR.


RESULTS
(1)Synoviocytes of AA rats proliferated more markedly than those of control rats (P<0.01), and exhibited a tumor-like proliferation. (2)The mRNAs of C-myc and ODC genes were obviously higher in synovial tissues of AA rats than those of control rats.


CONCLUSIONS
The tumor-like proliferation of synoviocytes from AA rat joint might be related to the increased mRNAs of C-myc and ODC genes.
Department of Immunology, Beijing University of Chinese Medicine, Beijing 100029, China. mengming127@sina.com
Ming
M
Meng
Li-gang
LG
Gu
Qian
Q
Zhang
Hua
H
Xie
Qing-guo
QG
Wang
D000818:Animals; D000843:Ankle Joint / Q000473:pathology; D001169:Arthritis, Experimental / Q000235:genetics* / Q000473:pathology*; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000473:pathology; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D008297:Male; D009369:Neoplasms / Q000473:pathology*; D009955:Ornithine Decarboxylase / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000473:pathology*
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase