The variation of the novel allele HLA-B*0739 suggests low alloreactivity when mismatched with HLA-B*0702.
We here report the identification of a new HLA-B*07 allele in a male Caucasian. The new allele was initially typed as B*0713 by sequence-specific primed PCR. Because of the infrequence of that allele, a sequencing-based typing was performed to confirm that result. This yielded the detection of the novel allele. It is closest to B*070201, while it differs from B*0713 in 12 positions in exon 2. Compared to B*070201, the new variant is characterized by a non-synonymous nucleotide exchange (C-->T) at nucleotide position 118 of exon 2. Previously, this was considered a constant position, suggesting that it is likely to be caused by a single-point mutation. It results in the amino acid exchange Ala-->Val at position 40 of the mature polypeptide. As this position is located in an outer loop of the HLA molecule, it is highly unlikely to affect peptide binding or T-cell receptor interaction. Thus, the newly found allele should have a low alloreactive potential in case of a mismatch to the most common HLA-B allele B*0702.
Department of Transfusion Medicine, Hannover Medical School, Hannover, Germany.
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Blasczyk
D000483:Alleles; D001483:Base Sequence; D005091:Exons; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000235:genetics*; D015793:HLA-B7 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D044465:White People
D015235:HLA-B Antigens; C477444:HLA-B*07:02 antigen; C503971:HLA-B*07:39 antigen; D015793:HLA-B7 Antigen; D010455:Peptides