Sensitivity and specificity of a rapid rRNA gene probe assay for simultaneous identification of Staphylococcus aureus and detection of mecA.
rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded against false-negative mecA results.
Kaplan
Shannon
S
Gen-Probe Incorporated, 10210 Genetic Center Drive, San Diego, CA 92121-4362, USA.
Marlowe
Elizabeth M
EM
Hogan
James J
JJ
Doymaz
Mehmet
M
Bruckner
David A
DA
Simor
Andrew E
AE
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000378:metabolism*; D015342:DNA Probes; D020459:Genes, rRNA; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D013997:Time Factors
D001426:Bacterial Proteins; D003030:Coagulase; D015342:DNA Probes; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus