Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
[reaction: see text] We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha(C)U). An efficient photoligation was produced by photoirradiation of an ODN containing alpha(C)U at the 3' end with an ODN containing thymine at the 5' end in the presence of a template ODN. This photoligation method is a new and efficient way to synthesize branched ODNs.
Ogino
Masayuki
M
The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa.
Yoshimura
Yoshinaga
Y
Nakazawa
Akio
A
Saito
Isao
I
Fujimoto
Kenzo
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry; D013698:Templates, Genetic
C509569:5-cyanovinyldeoxyuridine; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine