Effect of ultrasonic scaling on salivary lactate dehydrogenase.
OBJECTIVE
To determine Lactate Dehydrogenase Activity (LDHA) in whole saliva in individuals with periodontal disease and the effect of ultrasonic scaling on this enzyme activity.


METHODS
A study group of 50 patients with PD (Community Periodontal Index of Treatment Needs > or = 3) was selected at random. Half of the patients (n=25) received ultrasonic periodontal treatment (Group 1). In the other 25 patients with PD the ultrasonic scaling was not performed (Group 2). A control group was selected (Group 3) consisting of 25 adults without PD (Community Periodontal Index of Treatment Needs = 0), with age and gender distribution similar to the study group. LDHA determinations were carried out with a Cobas Mira Plus II autoanalyzer pursuant to the recommendations of the French Society of Clinical Biology. A baseline determination of LDHA was carried out on all the participants and a second determination was performed 3 months later.


RESULTS
Baseline determination of LDHA was higher in patients with PD than in the control group. These differences were statistically significant (P< 0.001). LDHA was significantly reduced in patients with PD after ultrasonic scaling with respect to baseline determination (P< 0.0001). LDHA differences between Groups 2 and 3 were statistically significant (P< 0.0001), but it was not so between Groups 1 and 3.
Stomatology Department, School of Medicine and Dentistry, Santiago de Compostela University, Spain.
Victor Alonso
VA
de la Peña
Pedro Diz
PD
Dios
Ignacio
I
Rodríguez-Nuñez
Santiago
S
Rodríguez-Segade
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use; D003742:Dental Devices, Home Care; D012534:Dental Scaling; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes / Q000627:therapeutic use; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology* / Q000628:therapy; D012463:Saliva / Q000201:enzymology*; D007507:Therapeutic Irrigation / Q000295:instrumentation; D014099:Toothbrushing / Q000295:instrumentation; D014100:Toothpastes / Q000627:therapeutic use; D014464:Ultrasonic Therapy
D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D014100:Toothpastes; D007770:L-Lactate Dehydrogenase; D002710:Chlorhexidine