Characterization of Lactobacillus strains and monitoring by RAPD-PCR in controlled fermentations of "Almagro" eggplants.
The characterization of 23 Lactobacillus strains was performed. The strains were assayed for biogenic amine-forming capacity, hydrogen peroxide production, pectin esterase, cellulase and polygalacturonase production, growth rate, acidifying capacity and salt tolerance. Three strains were selected which belonged to the species, Lactobacillus brevis, Lactobacillus plantarum and Lactobacillus fermentum. Different starter cultures prepared as combinations of these three strains were assayed in pilot scale fermentations and Randomly Amplified Polymorphic DNA (RAPD) analysis, using a previously selected random primer, was applied for monitoring the inoculated strains. The course of fermentations was similar in all batches but sensorial analysis of eggplants fermented using a mixed culture of the three strains displayed the best results, and no differences were obtained when compared with commercial eggplants.
Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias del Medio Ambiente, Avda. Carlos III s/n, 45071 Toledo, Spain. ssprieto@amb-to-uclm.es
Susana
S
Seseña
Isabel
I
Sánchez
Llanos
L
Palop
D001679:Biogenic Amines / Q000096:biosynthesis; D005285:Fermentation; D005516:Food Microbiology; D005524:Food Technology; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D052196:Levilactobacillus brevis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D052197:Limosilactobacillus fermentum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D011157:Population Dynamics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D032323:Solanum melongena / Q000382:microbiology*; D013045:Species Specificity
D001679:Biogenic Amines; D006861:Hydrogen Peroxide