Aspergillus nidulans uvsBATR and scaANBS1 genes show genetic interactions during recovery from replication stress and DNA damage.
The ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsB(ATR) and the Mre11 complex (scaA(NBS1)). We demonstrate that there is an epistatic relationship between uvsB(ATR), the homolog of the ATR/MEC1 gene, and scaA(NBS1), the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaA(NBS1) expression during recovery from replication stress depends on uvsB(ATR). In addition, we also show that the formation of UvsC foci during recovery from replication stress is dependent on both uvsB(ATR) and scaA(NBS1) function. Furthermore, ScaA(NBS1) is also dependent on uvsB(ATR) for nuclear focus formation upon the induction of DNA double-strand breaks by phleomycin. Our results highlight the extensive genetic interactions between UvsB and the Mre11 complex that are required for S-phase progression and recovery from DNA damage.
Fagundes
Marcia Regina von Zeska Kress
MR
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil.
Semighini
Camile P
CP
Malavazi
Iran
I
Savoldi
Marcela
M
de Lima
Joel Fernandes
JF
de Souza Goldman
Maria Helena
MH
Harris
Steven D
SD
Goldman
Gustavo Henrique
GH
D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D004261:DNA Replication / Q000235:genetics* / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000502:physiology*; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D016196:S Phase / Q000235:genetics; D014466:Ultraviolet Rays
D011494:Protein Kinases