Antibody response to a non-conserved C-terminal part of human histone deacetylase 3 in colon cancer patients.
Antibodies to cancer antigens can often be detected in the sera of patients, although the mechanism of the underlying humoral immune response is poorly understood. Using immunoscreening of tumor-derived cDNA expression libraries (SEREX), we identified human histone deacetylase 3 (HDAC3) as serologically defined antigen in colon cancer. Closely related HDAC1 and HDAC2 do not elicit humoral response in colon cancer patients. We show that the C-terminal region of HDAC3 protein lacking the homology to other Class I HDAC contains at least 3 distinct B-cell epitopes that are recognized by the serum antibodies. HDAC3 in combination with other SEREX antigens may become a useful molecular biomarker with diagnostic or prognostic value for a subset of colon cancer patients.
Basic Research Program, SAIC-Frederick, and Laboratory of Molecular Immunoregulation, Center for Cancer Research, NCI Frederick, Frederick, MD, USA.
Yuriy V
YV
Shebzukhov
Ekaterina P
EP
Koroleva
Svetlana V
SV
Khlgatian
Pavel V
PV
Belousov
Ksenia E
KE
Kuz'mina
Boris V
BV
Radko
Fanny
F
Longpre
Maria A
MA
Lagarkova
Tatiana S
TS
Kadachigova
Olga V
OV
Gurova
Andrey A
AA
Meshcheryakov
Mikhail R
MR
Lichinitser
Alexander
A
Knuth
Elke
E
Jager
Dmitry V
DV
Kuprash
Sergei A
SA
Nedospasov
D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000276:immunology*; D017931:DNA Primers; D000939:Epitopes / Q000276:immunology; D006655:Histone Deacetylases / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D000099186:Histone Deacetylase 3
D000912:Antibodies, Neoplasm; D017931:DNA Primers; D000939:Epitopes; D006655:Histone Deacetylases; D000099186:Histone Deacetylase 3