[Study on detection of samples of Rh-weak D and Del].
To study the detection of weak D and Del from samples initially screened RhD(-), RhD phenotype was initially screened by routine serological test, out of which weak D phenotype was detected by indirect antiglobulin test (IAT) and Del phenotype was detected by chloroform-trichloroethylene absorption-elution test. The results showed that 56 samples were RhD(-) confirmed by routine serology test, which were screened out of 26 200 donors, among them 5 samples were typed as weak D by IAT and 9 cases samples were typed as Del by absorption-elution test. In conclusion, the samples which typed as RhD(-) by routine serological test must be identified by IAT and chloroform-trchloroethylene absorption test is order to detect weak D and Del phenotype. It is important for clinical transfusion safely.
Wang
Xiao-Zhu
XZ
Laboratory of Blood Transfusion, Qinhuangdao Blood Center, Qinhuangdao 066001, China.
Lan
Jiong-Cai
JC
Wu
Xu-Hua
XH
Zhou
Hua-You
HY
Liu
Wei-Jia
WJ
Liu
Dan
D
Qi
Cun-Sheng
CS
Zeng
Feng-Qin
FQ
Du
Ke-Qing
KQ
D001782:Blood Donors; D001788:Blood Grouping and Crossmatching / Q000379:methods / Q000592:standards; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D012204:Rh-Hr Blood-Group System / Q000097:blood / Q000276:immunology*
D000939:Epitopes; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen