Induction of expression of bovine leukemia virus (BLV) in blood taken from BLV-infected cows without removal of plasma.
The expression of bovine leukemia virus (BLV) is blocked at the transcriptional level during the so-called latency period. However, when peripheral blood mononuclear cells and B lymphocytes are isolated from BLV-infected animals and incubated in the presence of activating reagents, such as phorbol ester, the expression of BLV is markedly enhanced. Such "reactivation" is thought to play a crucial role in the spread of BLV from infected to uninfected cattle. In the present study, we found that the expression of BLV in samples of whole blood from BLV-infected cattle was activated immediately upon incubation at 37 degrees C and that such activation did not require the addition of any exogenous factors except for anticoagulants or the removal of blood cells from plasma. The expression of BLV was repressed by an inhibitor of protein kinase C (PKC), namely, H-7, and by a membrane-permeable chelator of Ca2+ ions, BAPTA/AM. We also found that several isotypes of PKC were translocated immediately from the cytoplasm to the membrane fraction upon incubation of whole blood at 37 degrees C. Our data suggest that the actual collection of blood and pathways that involve PKC and Ca2+ might play important roles in the reactivation of expression of BLV in blood from infected cattle.
Retrovirus Research Unit, RIKEN, Wako, Saitama 351-0198, Japan.
Shigeru
S
Tajima
Yoko
Y
Aida
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D051696:Activating Transcription Factors / Q000378:metabolism; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001769:Blood / Q000821:virology*; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D002614:Chelating Agents / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D016583:Enzootic Bovine Leukosis / Q000821:virology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D005814:Genes, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D012367:RNA, Viral / Q000097:blood; D014775:Virus Activation
D051696:Activating Transcription Factors; D000998:Antiviral Agents; D001798:Blood Proteins; D002614:Chelating Agents; D017362:Cyclic AMP Response Element-Binding Protein; D004791:Enzyme Inhibitors; D012367:RNA, Viral; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C