Fast screening target sites for RNA interference using a cell-free system.
A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but which are more time-consuming than this present method.
Mei
Huang
H
Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China.
Fang-Jun
Jia
J
Chong-Yong
Li
L
Yi-Ping
Li
L
Li-He
Guo
G
D001709:Biotechnology / Q000379:methods*; D015152:Blotting, Northern; D002474:Cell-Free System; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014162:Transfection
D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins