Interactions between catalytic and inhibitory subunits of PDE6.
Rod and cone photoreceptor guanosine 3'5'-cyclic-monophosphate phosphodiesterases (PDEs) are classified into the PDE6 family of cyclic nucleotide PDEs. A unique feature of the PDE6 enzymes is the presence of inhibitory gamma-subunits (Pgamma). The inhibitory interaction between Pgamma and the rod PDE6alphabeta catalytic subunits is critically important for understanding the mechanism of phototransduction. Recent insights into the molecular interface between Pgamma and PDE6alphabeta have been achieved using mutagenesis of Pgamma, fluorescence labeling, and crosslinking approaches.
Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, USA.
Nikolai O
NO
Artemyev
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D005136:Eye Proteins; D006801:Humans; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics; D016296:Mutagenesis
D003432:Cross-Linking Reagents; D005136:Eye Proteins; D009097:Multienzyme Complexes; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; C410522:PDE6A protein, human; C517431:PDE6B protein, human