Effect of nano cadmium sulfide on the electron transfer reactivity and peroxidase activity of hemoglobin.
Hemoglobin (Hb) is immobilized with cadmium sulfide (CdS) nanoparticles (NPs) on pyrolytic graphite (PG) electrode to characterize the electrochemical reactivity and peroxidase activity of the protein. The result demonstrates that fine redox waves of Hb can be achieved after this protein is entrapped in CdS NPs. Meanwhile, the protein can exhibit nice catalytic activity towards hydrogen peroxide (H2O2). Linear relationship between the reductive peak current and the H2O2 concentration has been obtained from 5.0 x 10(-6) to 4.0 x 10(-4) mol/L, on the basis of which a new kind of H2O2 biosensor might be developed in the future.
Zhou
Hui
H
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
Gan
Xin
X
Liu
Tao
T
Yang
Qianlu
Q
Li
Genxi
G
D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D049329:Nanostructures; D013440:Sulfides / Q000737:chemistry*
D019187:Cadmium Compounds; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D013440:Sulfides; C034939:cadmium sulfide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase