Constituents of Holothuroidea, 15. Isolation of ante-iso type regio-isomer on long chain base moiety of glucocerebroside from the sea cucumber Holothuria leucospilota.
An ante-iso type regio-isomer on the long chain base moiety of a glucocerebroside, HLC-2-A, has been isolated from its parent glucocerebroside molecular species HLC-2 composed of iso and ante-iso isomers, from the less polar lipid fraction of a chloroform/methanol extract of the sea cucumber Holothuria leucospilota. Reverse-phase HPLC that included a recycling system was effective in separating the regio-isomer from its counterpart, revealing a very close resemblance in structure. Other typical glucocerebroside molecular species HLC-1 and HLC-3 were obtained together with HLC-2. The structures of these glucocerebrosides were determined on the basis of chemical and spectroscopic evidence.
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Koji
K
Yamada
Noriko
N
Wada
Hiroyuki
H
Onaka
Rei
R
Matsubara
Ryuichi
R
Isobe
Masanori
M
Inagaki
Ryuichi
R
Higuchi
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D005963:Glucosylceramides / Q000737:chemistry / Q000302:isolation & purification*; D047332:Holothuria / Q000737:chemistry*
D005963:Glucosylceramides