[Microbiological evaluation of gastroscope decontamination by electrolysed acid water (Clentop WM-1)].
BACKGROUND
The manual disinfection of endoscopes with glutharaldeyde is widely employed. The great routine in gastroenteroscopy services, low number of equipment and the lack of technical knowledge about the decontamination processes are factors that stimulate the inadequate endoscope disinfection, intensifying the risk of transmission of microorganisms. The electrolysed acid water has been effective in the inactivation and destruction of microorganisms.


OBJECTIVE
The purpose of this investigation was to verify the microbicidal efficiency of electrolyzed acid water (Cleantop WM-1) to decontaminate gastroscopes after their using in patients.


METHODS
Samples from biopsy channel of flexible endoscopes collected after patient use (n = 20) and after disinfection (n = 20) were cultivated in tryptic soy agar, MacConkey agar and Sabouraud dextrose agar.


RESULTS
Seventeen of the 20 samples collected after patients examination yielded gram-negative bacilli, gram-positive coccus and yeast cells in contamination of 3 to 5 log10 ufc/mL. Microbial growth was not verified in samples collected after the decontamination process. Conclusion - In this preliminary study, the mechanical disinfection carried through the Cleantop device with electrolyzed acid water showed satisfactory results for the elimination of microorganisms and time optimization in the reprocessing of gastroscopes.
Machado
Alexandre Paulo
AP
Departamento de Microbiologia, Immunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP.
Fischman
Olga
O
Geocze
Stephan
S
D003666:Decontamination / Q000295:instrumentation / Q000379:methods; D004202:Disinfectants; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D020699:Gastroscopes / Q000382:microbiology*; D006801:Humans; D006861:Hydrogen Peroxide
D004202:Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide