HR2 haplotype in Arab population and patients with venous thrombosis in Kuwait.
BACKGROUND
Venous thromboembolism (VTE) occurs due to a number of hereditary and acquired disorders of hemostasis. A recently identified polymorphism in factor V gene (A4070G; named HR2) has been reported to be a possible risk factor for the development of VTE, with a high prevalence of 9.5%-15.2% in patients of different ethnic groups in different parts of the world. However, the prevalence of HR2 has not yet been tested in VTE patients of Arab ethnicity.


OBJECTIVE
To study the prevalence and possible risk of HR2 haplotype in Arabs.


METHODS
Exactly 188 VTE patients and 100 healthy subjects, all being of Arab ethnicity, were examined for HR2 using Polymerase chain reaction, restriction fragment length polymorphism and agarose gel electrophoresis.


RESULTS
Data showed that 31 patients and seven healthy subjects had HR2 haplotype, with a prevalence of 16.5% and 7%, respectively. Furthermore, 43 patients (22.9%) had more than one risk factor for VTE.


CONCLUSIONS
The prevalence of HR2 in Arabs is quite high, with a 2.62-fold greater risk of developing VTE. Moreover, coexistence of two or more genetic/acquired defects of VTE is quite common in Arab patients.
Jadaon
M M
MM
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University, Kuwait. mehrez@hsc.kuniv.edu.kw
Dashti
A A-R
AA
D018912:Arabs; D016022:Case-Control Studies; D004587:Electrophoresis, Agar Gel; D005165:Factor V / Q000235:genetics*; D005260:Female; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D007730:Kuwait; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012306:Risk; D012307:Risk Factors; D020246:Venous Thrombosis / Q000453:epidemiology* / Q000235:genetics*
D005165:Factor V