Characterization of a long terminal repeat region from an infectious Indian HIV type 2 isolate.
An infectious Indian human immunodeficiency virus type 2 isolate from Mumbai, propagated in this laboratory, was found to bear an unusually short long terminal repeat (LTR) region. Complete sequencing of the 601 bp LTR indicated a loss of around 250 nucleotide pairs from the unique 3' (U3) region as compared to other well-characterized HIV-2 isolates. Phylogenetic analysis of this LTR shows closest relatedness to the Guinea-Bissau subtype A isolates HIV-2(CAM2) and HIV-2(ALI). The LTR from the biologically active infectious clone with the observed deletion contained all functionally relevant promoter and polyadenylation sequences.
Tamhane
Mayur
M
Virology Laboratory, Tata Memorial Centre, Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Kharghar, Navi Mumbai-410 208, India.
Mukhopadhyaya
Rita
R
Mukhopadhyaya
Robin
R
D001483:Base Sequence; D015658:HIV Infections / Q000821:virology*; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA