AFM studies of water-soluble wheat arabinoxylans--effects of esterase treatment.
The degradation products of water-soluble wheat arabinoxylans treated with Aspergillus niger ferulic acid esterase (FAEA-able to cleave 5,5'- and 8-O-4'-ferulic acid dimers) have been characterised by atomic force microscopy (AFM) and size exclusion chromatography. The AFM images of arabinoxylans confirmed that a small proportion ( approximately 15%) of the population of arabinoxylan molecules contain xylan-based branches attached to the xylan-based backbone. Treatment with FAEA reduced the contour length of the molecules suggesting that certain dimeric ferulic acid linkages may play a previously unconfirmed role in the elongation of arabinoxylans. Overnight treatment with FAEA led to a reduction in the density of branches suggesting that they may also be linked to the backbone through phenolic linkages.
Adams
Elizabeth L
EL
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, UK.
Kroon
Paul A
PA
Williamson
Gary
G
Morris
Victor J
VJ
D001234:Aspergillus niger / Q000201:enzymology; D002138:Calibration; D002236:Carbohydrate Conformation; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry; D004950:Esterases / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D010426:Pentosan Sulfuric Polyester / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*; D014990:Xylans / Q000737:chemistry*
D003373:Coumaric Acids; D011994:Recombinant Proteins; D014990:Xylans; D010426:Pentosan Sulfuric Polyester; C085118:arabinoxylan; C004999:ferulic acid; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase