RETRACTED: A magnetic nanoprobe technology for detecting molecular interactions in live cells.
Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Jaejoon
J
Won
Mina
M
Kim
Yong-Weon
YW
Yi
Young Ho
YH
Kim
Neoncheol
N
Jung
Tae Kook
TK
Kim
D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002468:Cell Physiological Phenomena; D002470:Cell Survival; D004705:Endocytosis; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D008280:Magnetics; D018613:Microscopy, Confocal; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D049329:Nanostructures; D009842:Oligopeptides / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D045663:Quantum Dots; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D019809:Streptavidin; D016559:Tacrolimus / Q000378:metabolism; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D044844:beta-Transducin Repeat-Containing Proteins / Q000378:metabolism
D005456:Fluorescent Dyes; D020672:I-kappa B Proteins; D015335:Molecular Probes; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D009842:Oligopeptides; D011506:Proteins; D011993:Recombinant Fusion Proteins; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C108637:aspartyl-glutamyl-valyl-aspartal; D044844:beta-Transducin Repeat-Containing Proteins; D000072000:NF-KappaB Inhibitor alpha; D019809:Streptavidin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D016650:Fluorescein-5-isothiocyanate; D016559:Tacrolimus