[Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials].
OBJECTIVE
To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.


METHODS
Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.


RESULTS
10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.


CONCLUSIONS
The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.
Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Li-jun
LJ
Zhang
Jie-feng
JF
Cui
Yin-kun
YK
Liu
Yun-song
YS
Zhu
Hai-yan
HY
Song
Hua-li
HL
Shen
Ju-tao
JT
Feng
Zhi
Z
Dai
Jie
J
Chen
Rui-xia
RX
Sun
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis; D019000:Phosphotyrosine / Q000032:analysis*
D009363:Neoplasm Proteins; D019000:Phosphotyrosine