Spectrophotometric method for polyphenols analysis: prevalidation and application on Plantago L. species.
The prevalidation strategy was applied to evaluate UV-vis spectrophotometric procedure with Folin-Ciocalteu's reagent for polyphenols determination. Favourable prevalidation characteristics verified this procedure as a valuable tool in polyphenols analysis and it was successfully applied for determination of total polyphenols and tannins in leaves, stems and flowers of Plantago L. species growing in Croatia. The results showed the variety of total polyphenols content between different plant parts (leaves: up to 10.15%; stems: up to 4.34% and flowers: up to 5.56%). The content of tannins in stems was from 0.28% to 1.00%, while leaves and flowers contained tannins in concentrations of 2.26% and 2.21%, respectively. The results of polyphenols determination were evaluated by using multivariate analysis (UPGMA and PCA) as a contribution to elucidation of relations between different taxa of genus Plantago L.
Grubesić
R Jurisić
RJ
Department of Analytics and Control of Medicines, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacića 1, HR-10000 Zagreb, Croatia.
Vuković
J
J
Kremer
D
D
Vladimir-Knezević
S
S
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004345:Drug Industry / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D008962:Models, Theoretical; D015999:Multivariate Analysis; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010943:Plantago / Q000378:metabolism*; D059808:Polyphenols; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013634:Tannins / Q000032:analysis; D014466:Ultraviolet Rays
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013634:Tannins