[Changes of endogenous ouabain in rat serum and certain tissues after exposure to simulated weightlessness].
OBJECTIVE
To investigate the changes of endogenous ouabain (EO) in rat serum and some tissues after exposure to simulated weightlessness and to investigate its possible pathophysiology.


METHODS
Male Wistar rats were randomly divided into control group (Con) and 1 week tail-suspension group (TS). Enzyme-linked immunosorbent assay (ELISA) was used to detect the content of EO in serum, hypothalamus, pituitary, adrenal gland, kidney, heart and liver.


RESULTS
Compared with Con, EO increased significantly in serum, hypothalamus, adrenal gland and kidney after tail suspension (P<0.05).


CONCLUSIONS
Simulated weightlessness induced changes of EO in serum and some tissues, which may have some effects on the regulation of hydro-electrolyte metabolism and cardiovascular functions.
Department of Medicine, Xi'an Jiaotong University, Xi'an Shannxi, China. liuzx918@sohu.com
Zhao-xia
ZX
Liu
Guang-dao
GD
Gao
Yong-jie
YJ
Hu
De-sheng
DS
Wang
Da-wei
DW
WU
Ming
M
Yuan
Zhi-li
ZL
Li
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D019417:Hindlimb Suspension; D007031:Hypothalamus / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010042:Ouabain / Q000097:blood / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018474:Weightlessness Simulation
D010042:Ouabain