Spatial learning and long-term potentiation of mutant mice lacking D-amino-acid oxidase.
We evaluated the role of D-amino-acid oxidase on spatial learning and long-term potentiation (LTP) in the hippocampus, since this enzyme metabolizes D-amino-acids, some of which enhance the N-methyl-D-aspartate receptor functions. The Morris water maze learning and the LTP in the CA1 area of the hippocampal slice were observed in wild-type mice and mutant mice lacking D-amino-acid oxidase. The mutant mice showed significantly shorter platform search times in the water maze and significantly larger hippocampal LTPs than the wild-type mice. These results suggest that the abundant D-amino-acids in the mutant mouse brain facilitate hippocampal LTP and spatial learning.
Department of Physiology and Biological Information, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu, Tochigi 321-0293, Japan. maekawa@dokkyomed.ac.jp
Masao
M
Maekawa
Masashi
M
Watanabe
Shigeki
S
Yamaguchi
Ryuichi
R
Konno
Yuuichi
Y
Hori
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D003605:D-Amino-Acid Oxidase / Q000172:deficiency*; D004558:Electric Stimulation / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D066298:In Vitro Techniques; D007858:Learning / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D017774:Long-Term Potentiation / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques / Q000379:methods; D017966:Pyramidal Cells / Q000187:drug effects / Q000528:radiation effects; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013550:Swimming / Q000502:physiology
D018691:Excitatory Amino Acid Antagonists; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D003605:D-Amino-Acid Oxidase