Bone marrow stromal cells generate muscle cells and repair muscle degeneration.
Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow.
Dezawa
Mari
M
Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoecho, Sakyo-ku, Kyoto, 606-8501 Japan. dezawa@anat2.med.kyoto-u.ac.jp
Ishikawa
Hiroto
H
Itokazu
Yutaka
Y
Yoshihara
Tomoyuki
T
Hoshino
Mikio
M
Takeda
Shin-ichi
S
Ide
Chizuka
C
Nabeshima
Yo-ichi
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002459:Cell Fusion; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000032:analysis; D006801:Humans; D051379:Mice; D018101:Mice, Inbred mdx; D008819:Mice, Nude; D032342:Muscle Cells / Q000166:cytology*; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D009124:Muscle Proteins / Q000032:analysis; D018482:Muscle, Skeletal / Q000166:cytology; D009135:Muscular Diseases / Q000628:therapy*; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D020880:Neuregulins / Q000494:pharmacology; D051763:PAX7 Transcription Factor; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D014162:Transfection
D018398:Homeodomain Proteins; D009124:Muscle Proteins; D020880:Neuregulins; D051763:PAX7 Transcription Factor; C497321:PAX7 protein, human; C497322:Pax7 protein, mouse; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D005576:Colforsin