Analysis of semicarbazide in baby food by liquid chromatography tandem mass spectrometry (LC-MS-MS)--in-house method validation.
A method for detection of semicarbazide (SEM) in baby food was validated. SEM was extracted with hydrochloric acid and derivatised with 2-nitrobenzaldehyde, using [15N2,13C] semicarbazide as internal standard. The extract was neutralised, purified on a solid phase extraction cartridge and SEM was determined by reversed phase LC-MS-MS. Linearity was demonstrated in the ranges from 0.1 ng ml(-1) to 1 ng ml(-1) and from 2 ng ml(-1) to 80 ng ml(-1). Matrix effects were non significant for meat-based and significant for apple and rice-based baby foods, in both ranges. Mean recoveries ranged from 87.8% to 107.2% with relative standard deviation from 0.2% to 9.1%, considering both ranges. Limits of detection and quantification were 0.1 microg kg(-1) and 0.25 microg kg(-1), respectively. The results of the validation process demonstrated the method suitability for use in food control.
de Souza
Scheilla V C
SV
Universidade Federal de Minas Gerais (UFMG), Faculdade de Farmácia (FAFAR), Departamento de Alimentos, Av. AntOnio Carlos 6627, Campus da UFMG, Pampulha, Zip-Code 31.270-010, Belo Horizonte, MG, Brazil.
Junqueira
Roberto G
RG
Ginn
Richard
R
D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012663:Semicarbazides / Q000032:analysis*; D012680:Sensitivity and Specificity
D012663:Semicarbazides; C010059:carbamylhydrazine