Dimethyl sulfone in human cerebrospinal fluid and blood plasma confirmed by one-dimensional (1)H and two-dimensional (1)H-(13)C NMR.
(1)H-NMR spectroscopy at 500 MHz was used to confirm that a previously unidentified singlet resonance at 3.14 ppm in the spectra of cerebrospinal fluid and plasma samples corresponds to dimethyl sulfone (DMSO(2)). A triple resonance inverse cryogenic NMR probe, with pre-amplifier and the RF-coils cooled to low temperature, was used to obtain an (1)H-(13)C HSQC spectrum of CSF containing 8 microM (753 ng/ml) DMSO(2). The (1)H-(13)C correlation signal for DMSO(2) was assigned by comparison with the spectrum from an authentic reference sample. In plasma and CSF from healthy controls, the concentration of DMSO(2) ranged between 0 and 25 micromol/l. The concentration of DMSO(2) in plasma from three of four patients with severe methionine adenosyltransferase I/III (MAT I/III) deficiency was about twice the maximum observed for controls. Thus, DMSO(2) occurs as a regular metabolite at low micromolar concentrations in cerebrospinal fluid and plasma. It derives from dietary sources, from intestinal bacterial metabolism and from human endogenous methanethiol metabolism.
Engelke
Udo F H
UF
Radboud University Nijmegen Medical Centre, Laboratory of Pediatrics and Neurology, NL-6500 HB Nijmegen, The Netherlands.
Tangerman
Albert
A
Willemsen
Michèl A A P
MA
Moskau
Detlef
D
Loss
Sandra
S
Mudd
S Harvey
SH
Wevers
Ron A
RA
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid / Q000378:metabolism; D002247:Carbon Isotopes; D002648:Child; D002675:Child, Preschool; D004121:Dimethyl Sulfoxide; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008659:Metabolic Diseases / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D008875:Middle Aged; D009422:Nervous System Diseases / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D011522:Protons; D013450:Sulfones / Q000097:blood* / Q000134:cerebrospinal fluid*
D015415:Biomarkers; D002247:Carbon Isotopes; D011522:Protons; D013450:Sulfones; C025910:dimethyl sulfone; D004121:Dimethyl Sulfoxide