Ergosterol peroxide from flowers of Erigeron annuus L. as an anti-atherosclerosis agent.
Flowers of Erigeron annuus L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H2O. Repeated silica gel and ODS column chromatography of the EtOAc fraction led to the isolation of a sterol, through activity-guided fractionation, using ACAT inhibitory activity measurements. From the physico-chemical data, including NMR, MS, and IR, the chemical structure of the compound was determined to be an ergosterol peroxide (1), which has been isolated for the first time from this plant. This compound exhibited hACAT-1 and Lp-PLA2 inhibitory effects, with inhibitory values of 51.6 +/- 0.9 and 51.7 +/- 1.2%, at a treatment concentration of 0.23 mM.
Kim
Dong-Hyun
DH
Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Jung
Sung Je
SJ
Chung
In-Sik
IS
Lee
Youn-Hyung
YH
Kim
Dae-Keun
DK
Kim
Sung-Hoon
SH
Kwon
Byoung-Mog
BM
Jeong
Tae-Sook
TS
Park
Mi-Hyun
MH
Seoung
Nak-Sul
NS
Baek
Nam-In
NI
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D001161:Arteriosclerosis / Q000188:drug therapy*; D019659:Asteraceae / Q000737:chemistry*; D002784:Cholesterol; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D051381:Rats; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; D010741:Phospholipases A; D054467:Phospholipases A2; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol