Disk diffusion testing using Candida sp. colonies taken directly from CHROMagar Candida medium may decrease time required to obtain results.
We compared results of disk diffusion antifungal susceptibility testing from Candida sp. strains passaged on CHROMagar and on potato dextrose agar. The overall categorical agreements for fluconazole and voriconazole disk testing were 95% and 98% with 0% and 0.5% very major errors, respectively. Disk diffusion testing by the CLSI (formerly NCCLS) M44-A method can be performed accurately by taking inocula directly from CHROMagar.
Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA.
Michael
M
Klevay
Alex
A
Ebinger
Daniel
D
Diekema
Shawn
S
Messer
Richard
R
Hollis
Michael
M
Pfaller
D000362:Agar; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D002863:Chromogenic Compounds; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D013997:Time Factors
D000935:Antifungal Agents; D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar