Complex humoral immune response against a benign tumor: frequent antibody response against specific antigens as diagnostic targets.
There are numerous studies on the immune response against malignant human tumors. This study was aimed to address the complexity and specificity of humoral immune response against a benign human tumor. We assembled a panel of 62 meningioma-expressed antigens that show reactivity with serum antibodies of meningioma patients, including 41 previously uncharacterized antigens by screening of a fetal brain expression library. We tested the panel for reactivity with 48 sera, including sera of patients with common-type, atypical, and anaplastic meningioma, respectively. Meningioma sera detected an average of 14.6 antigens per serum and normal sera an average of 7.8 antigens per serum (P = 0.0001). We found a decline of seroreactivity with malignancy with a statistical significant difference between common-type and anaplastic meningioma (P < 0.05). We detected 17 antigens exclusively with patient sera, including 12 sera that were reactive against KIAA1344, 9 against natural killer tumor recognition (NKTR), and 7 against SRY (sex determining region Y)-box2 (SOX2). More than 80% of meningioma patients had antibodies against at least one of the antigens KIAA1344, SC65, SOX2, and C6orf153. Our results show a highly complex but specific humoral immune response against a benign tumor with a distinct serum reactivity pattern and a decline of complexity with malignancy. The frequent antibody response against specific antigens offers new diagnostic and therapeutic targets for meningioma. We developed a statistical learning method to differentiate sera of meningioma patients from sera of healthy donors.
Department of Human Genetics, Medical School, University of Saarland, Building 60, 66421 Homburg/Saar, Germany.
Nicole
N
Comtesse
Andrea
A
Zippel
Sascha
S
Walle
Dominik
D
Monz
Christina
C
Backes
Ulrike
U
Fischer
Jens
J
Mayer
Nicole
N
Ludwig
Andreas
A
Hildebrandt
Andreas
A
Keller
Wolf-Ingo
WI
Steudel
Hans-Peter
HP
Lenhof
Eckart
E
Meese
D000912:Antibodies, Neoplasm / Q000097:blood*; D000917:Antibody Formation / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D017931:DNA Primers; D016002:Discriminant Analysis; D015723:Gene Library; D006801:Humans; D008578:Meninges / Q000378:metabolism; D008579:Meningioma / Q000097:blood / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012698:Serologic Tests
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D017931:DNA Primers