Assessment of a platelet function analyser in horses: reference range and influence of a platelet aggregation inhibitor.
The objective of this study was to assess whether a new human platelet function analyser (the PFA-100) could be used to evaluate platelet function in horses and detect acetylsalicylic acid (ASA)-induced platelet dysfunctions. Citrated blood samples from 40 healthy horses were processed to obtain reference values for closure time (CT) using cartridges with collagen-ADP (CT-ADP) and collagen-epinephrine (CT-EPI) as platelet agonists. In addition, CT-ADP and CT-EPI were also measured before and 24 h after oral ASA administration in another 12 healthy horses. The sensitivity and specificity of the test were also determined. In normal horses, means+/-SD value for CT-ADP was 85.1+/-13.1 s (median, 82 s), and CT-EPI ranged from 158 to >300 s (median 291 s). Calculated reference ranges were 60.5-115.9 s and 158.5->300 s for CT-ADP and CT-EPI, respectively. Administration of ASA significantly (P<0.001) prolonged CT-ADP values from 91.0+/-13 to 113.5+/-14.4 s, and CT-EPI values were also significantly (P<0.008) prolonged after ASA administration. Sensitivity and specificity results for ADP cartridges showed that a prolonged CT value would be highly suggestive of a platelet aggregation inhibition. In conclusion, ADP cartridges can be used in horses to assess primary haemostasis and may be a valuable test for the detection of platelet aggregation inhibition.
Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona. didac.segura@uab.es
D
D
Segura
L
L
Monreal
Y
Y
Espada
J
J
Pastor
I
I
Mayós
J
J
Homedes
D000818:Animals; D001241:Aspirin / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D006736:Horses / Q000097:blood*; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage* / Q000097:blood; D010979:Platelet Function Tests / Q000295:instrumentation / Q000662:veterinary*; D011237:Predictive Value of Tests; D012016:Reference Values
D010975:Platelet Aggregation Inhibitors; D001241:Aspirin