A versatile vector system for multiple gene expression in plants.
Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.
Chung
Sang-Min
SM
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA.
Frankman
Ellen L
EL
Tzfira
Tzvi
T
D003001:Cloning, Molecular / Q000379:methods; D018506:Gene Expression Regulation, Plant; D005810:Multigene Family; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic / Q000235:genetics