Tissue microarrays: bridging the gap between research and the clinic.
Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced to a tissue microarray format and are being applied to preclinical drug development. In clinical research, tissue microarrays are applied at multiple levels: comprehensive analysis of samples in the context of a clinical trial or across a population. Tissue microarrays play a central role in translational research, facilitating the discovery of molecules that have potential roles in the diagnosis, prognosis and prediction of response to therapy.
Braunschweig
Till
T
Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4605, USA. braunsct@mail.nih.gov
Chung
Joon-Yong
JY
Hewitt
Stephen M
SM
D000818:Animals; D002460:Cell Line; D002986:Clinical Trials as Topic; D006801:Humans; D017403:In Situ Hybridization; D046228:Microarray Analysis / Q000379:methods*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D012107:Research Design
D012313:RNA