In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds.
In vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol exudation and less contamination. Following establishment phase, the small shoots emerged from explants were subcultured on MS medium supplemented with different combinations and concentrations of growth regulators. BA (1.0 mgl(-1)) and GA3 (0.5 mgl(-1)) combination showed highest multiplication rate (1:5), andcl also produced longer shoots. Two step rooting was done by transferring microcuttings to auxin free solid medium after root initiation in dark on 1/2 strength MS liquid medium containing IBA (0.5 mgl(-1) ). Rooted plantlets were transferred to peat containing paper cups and resulting plants of MM 111 acclimated successfully for transfer to field.
Department of Biotechnology, Dr YS Parmar University of Horticulture & Forestry. Nauni, Solan 173 230, India.
N
N
Kaushal
M
M
Modgil
M
M
Thakur
D R
DR
Sharma
D018929:Cell Culture Techniques / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques; D005638:Fruit; D007210:Indoleacetic Acids; D027845:Malus / Q000166:cytology* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism
D003470:Culture Media; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators