Application of preparative high-speed counter-current chromatography for separation of methyl gallate from Acer truncatum Bunge.
Preparative separation of methyl gallate in leaves extract of Acer truncatum Bunge was conducted using high-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-ethanol-water at volume ratios of 5:1:5 (v/v/v). In a single operation, 57.5 mg of methyl gallate was obtained from 120 mg of the extract. HPLC analyses of the counter-current chromatography (CCC) fraction revealed that the methyl gallate was having over 97% purity. Its structure was identified by 1H NMR and 13C NMR.
Department of Biology, The Graduate School of Chinese Academy of Sciences, Beijing 100049, China.
Xiaofeng
X
Ma
Linhuan
L
Wu
Yoichiro
Y
Ito
Weixi
W
Tian
D031002:Acer / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy
C052082:methyl gallate; D005707:Gallic Acid