Effects of increased cellular zinc levels on gene and protein expression in HT-29 cells.
High cellular zinc concentrations lead to impairments in ATP synthesis and cell cycle control particularly in neurons and epithelial cells. The molecular basis for these dysfunctions is still not fully elucidated. Here we analyzed the effects of a high zinc exposure (10 ppm) on gene and protein expression in the human epithelial cell line HT-29. Of the 1176 genes analyzed with cDNA arrays, nine differentially expressed genes were identified. Proteome analysis based on 1310 detected proteins identified 11 molecular targets. Most of the identified genes/proteins have not been linked to cellular zinc status before (e.g. PEC-60, R-ras3). More than half of the targets participate in ATP production or stress response. Therefore, it appears that higher zinc concentrations mediate their effects mainly via impairments in cellular energy metabolism and stress response.
Molecular Nutrition Unit, Technical University of Munich, 85350 Freising-Weihenstephan, Germany.
Birgit
B
Kindermann
Frank
F
Döring
Dagmar
D
Fuchs
Michael W
MW
Pfaffl
Hannelore
H
Daniel
D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology*
D011506:Proteins; D012333:RNA, Messenger; D019344:Lactic Acid; D015032:Zinc