Determining mutational status of immunoglobulin v genes in chronic lymphocytic leukemia: a useful prognostic indicator.
Determining the clonal origins of malignant B-cells will have an impact on disease understanding and management. In this regard, immunoglobulin variable (V) region gene analysis already is having a significant impact in delineating the tumor cell of origin. It can identify, among other features whether such a cell has undergone somatic mutation, which usually occurs within germinal centres. Remarkably, in chronic lymphocytic leukemia (CLL), the mutational status of V genes has allowed researchers to identify two subsets of disease, one originating from an unmutated B-cell with a markedly poorer disease outcome and the other from a mutated B-cell, which associates with long-term survival. The V gene status in CLL thus provides a robust indicator of disease outcome, which is beginning to shape clinical treatment. This chapter describes in detail the methodology for determining V gene usage in CLL, from acquisition of patient sample to generating the V-gene readout.
Sahota
Surinder S
SS
Cancer Sciences Division, Southampton University Hospitals NHS Trust, University of Southampton, UK.
Babbage
Gavin
G
Zojer
Niklas
N
Ottensmeier
Christian H
CH
Stevenson
Freda K
FK
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D011379:Prognosis
D004273:DNA, Neoplasm; D007135:Immunoglobulin Variable Region