A cDNA encoding diazepam-binding inhibitor/acyl-CoA-binding protein in Helicoverpa armigera: molecular characterization and expression analysis associated with pupal diapause.
The diazepam binding inhibitor (DBI) or the acyl-CoA-binding protein (ACBP) is a 9-10 kDa highly conserved multifunctional protein that plays important roles in GABA(A) receptor activity regulation, lipid absorption and steroidogenesis in various organisms. To study the functions of DBI/ACBP in insect development or diapause, we cloned the cDNA from Helicoverpa armigera (Har) utilizing rapid amplification of cDNA ends (RACE). By homology search, Har-DBI/ACBP is conserved with the DBI/ACBPs known from other insects. Northern blot analysis showed that DBI/ACBP gene expressed in nonneural and neural tissues. RT-PCR combined Southern blot analysis revealed that DBI/ACBP mRNA in the brain of nondiapause individual was much higher than that in the brain of diapausing insects. At early and middle stages of 6th instar larvae, the level of DBI/ACBP mRNA was higher in the midgut of diapause type than that in nondiapause type and low at late 6th instar larval stage and early pupal stage in both types. In the prothoracic gland (PG), DBI/ACBP expression appeared at a high level at middle and late stages of 6th larval instar in both nondiapause and diapause types, and declined after pupation. In vitro experiments revealed that DBI/ACBP mRNA in PG could be stimulated by synthetic H. armigera diapause hormone (Har-DH), suggesting that Har-DH may stimulate the PG to produce ecdysteroids by the DBI/ACBP signal pathway. By in vitro assay, we also found that FGIN-1-27, which has similar functions to DBI/ACBP in ecdysteroidogenesis, could induce PG ecdysteroidogenesis effectively, suggesting that DBI/ACBP regulates biosynthesis of ecdysteroids in PG. Thus, DBI/ACBP indeed plays a key role in metabolism and development in H. armigera.
Liu
Ming
M
Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, PR China.
Zhang
Tian-Yi
TY
Xu
Wei-Hua
WH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis*; D026261:Diazepam Binding Inhibitor / Q000737:chemistry / Q000378:metabolism*; D026461:Ecdysteroids / Q000096:biosynthesis; D007210:Indoleacetic Acids / Q000494:pharmacology; D007915:Lepidoptera / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D014018:Tissue Distribution
D018076:DNA, Complementary; D026261:Diazepam Binding Inhibitor; D026461:Ecdysteroids; D007210:Indoleacetic Acids; D012333:RNA, Messenger; C081306:N,N-di-n-hexyl-2-(4-fluorophenyl)indole-3-acetamide