Structure of human PNP complexed with ligands.
Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.
Programa de Pós-Graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
Fernanda
F
Canduri
Rafael Guimarães
RG
Silva
Denis Marangoni
DM
dos Santos
Mário Sérgio
MS
Palma
Luiz Augusto
LA
Basso
Diógenes Santiago
DS
Santos
Walter Filgueira
WF
de Azevedo
D001375:Azaguanine / Q000737:chemistry; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006147:Guanine / Q000737:chemistry; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D007288:Inosine / Q000737:chemistry; D008024:Ligands; D011684:Purine Nucleosides; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011744:Pyrimidinones / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry
D008024:Ligands; D011684:Purine Nucleosides; D011744:Pyrimidinones; D011758:Pyrroles; D006151:Guanosine; C113101:forodesine; D007288:Inosine; D006147:Guanine; D011683:Purine-Nucleoside Phosphorylase; D001375:Azaguanine