Allelic dosage analysis with genotyping microarrays.
Genomic alternations, including dosage and allelic imbalance, constitute a major basis of neoplastic and other genetic disorders. Using oligonucleotide genotyping microarrays, here we report the development and usage of an algorithm, called genome imbalance map (GIM) algorithm, for allelic as well as total gene dosage analysis. Using the GIM algorithm, global genome imbalance status at over 100,000 loci was simultaneously analyzed with unprecedented accuracy and allelic discrimination.
Genome Science Division, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Shumpei
S
Ishikawa
Daisuke
D
Komura
Shingo
S
Tsuji
Kunihiro
K
Nishimura
Shogo
S
Yamamoto
Binaya
B
Panda
Jing
J
Huang
Masashi
M
Fukayama
Keith W
KW
Jones
Hiroyuki
H
Aburatani
D000465:Algorithms; D002874:Chromosome Mapping / Q000379:methods*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D004314:Down Syndrome / Q000235:genetics*; D005240:Feasibility Studies; D018628:Gene Dosage; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity