Minimal sodium channel pore consisting of S5-P-S6 segments preserves intracellular pharmacology.
We studied the properties of a sodium channel comprised only of S5-P-S6 region of the rat sodium channel alpha-subunit Nav1.4 (micro1pore). Results obtained in HEK cell lines permanently transfected with the sodium channel alpha-subunit or with the micro1pore were compared with data of the native HEK cells. Sodium channel blockers, tetrodotoxin and tetracaine, protect cells transfected with the complete sodium channel against death produced by incubation with veratridine. Veratridine-induced cell death in cell lines expressing the micro1pore construct is antagonised by tetracaine, but not by tetrodotoxin. Whole-cell conductance also increases in the presence of veratridine in micro1pore transfected cells and tetracaine inhibits these currents. Our pharmacological and electrophysiological data suggest that micro1pore keeps binding sites for veratridine and tetracaine, but not for TTX, and reconstitutes the permeation pathway for Na+ ions.
Pincin
Chiara
C
Istituto di Biofisica, CNR, Genoa, Italy.
Ferrera
Loretta
L
Moran
Oscar
O
D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D042541:Intracellular Space / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009124:Muscle Proteins / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D062553:NAV1.4 Voltage-Gated Sodium Channel; D021122:Protein Subunits; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D013329:Structure-Activity Relationship; D013748:Tetracaine / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D009124:Muscle Proteins; D062553:NAV1.4 Voltage-Gated Sodium Channel; D021122:Protein Subunits; C106251:SCN4A protein, human; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013748:Tetracaine; D013779:Tetrodotoxin; D014701:Veratridine