Immunoglobulin M enzyme-linked immunosorbent assay using recombinant polypeptides for diagnosis of dengue.
We demonstrate that a mixture of four recombinant dengue virus E polypeptides corresponding to the N-terminal region of the envelope protein from all serotypes substitutes for standard antigens in two immunoglobulin M enzyme-linked immunosorbent assay formats with 100% concordance, making these polypeptides a useful and accessible reagent for serological diagnosis of dengue in endemic countries.
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, CA 94720-7360, USA.
Elsa
E
Videa
Maria Josefina
MJ
Coloma
Flavia Barreto
FB
Dos Santos
Angel
A
Balmaseda
Eva
E
Harris
D000914:Antibodies, Viral / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007075:Immunoglobulin M; D011994:Recombinant Proteins