Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise.
We hypothesized that an acute bout of strenuous, non-damaging exercise would increase rates of protein synthesis of collagen in tendon and skeletal muscle but these would be less than those of muscle myofibrillar and sarcoplasmic proteins. Two groups (n = 8 and 6) of healthy young men were studied over 72 h after 1 h of one-legged kicking exercise at 67% of maximum workload (W(max)). To label tissue proteins in muscle and tendon primed, constant infusions of [1-(13)C]leucine or [1-(13)C]valine and flooding doses of [(15)N] or [(13)C]proline were given intravenously, with estimation of labelling in target proteins by gas chromatography-mass spectrometry. Patellar tendon and quadriceps biopsies were taken in exercised and rested legs at 6, 24, 42 or 48 and 72 h after exercise. The fractional synthetic rates of all proteins were elevated at 6 h and rose rapidly to peak at 24 h post exercise (tendon collagen (0.077% h(-1)), muscle collagen (0.054% h(-1)), myofibrillar protein (0.121% h(-1)), and sarcoplasmic protein (0.134% h(-1))). The rates decreased toward basal values by 72 h although rates of tendon collagen and myofibrillar protein synthesis remained elevated. There was no tissue damage of muscle visible on histological evaluation. Neither tissue microdialysate nor serum concentrations of IGF-I and IGF binding proteins (IGFBP-3 and IGFBP-4) or procollagen type I N-terminal propeptide changed from resting values. Thus, there is a rapid increase in collagen synthesis after strenuous exercise in human tendon and muscle. The similar time course of changes of protein synthetic rates in different cell types supports the idea of coordinated musculotendinous adaptation.
Institute of Sports Medicine, Copenhagen, Bispebjerg Hospital, Denmark.
Benjamin F
BF
Miller
Jens L
JL
Olesen
Mette
M
Hansen
Simon
S
Døssing
Regina M
RM
Crameri
Rasmus J
RJ
Welling
Henning
H
Langberg
Allan
A
Flyvbjerg
Michael
M
Kjaer
John A
JA
Babraj
Kenneth
K
Smith
Michael J
MJ
Rennie
D000328:Adult; D002250:Carbon Radioisotopes; D003094:Collagen / Q000096:biosynthesis*; D015444:Exercise / Q000502:physiology*; D045782:Hemiterpenes; D006801:Humans; D007651:Keto Acids / Q000097:blood; D007930:Leucine / Q000097:blood; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D009210:Myofibrils / Q000378:metabolism; D009590:Nitrogen Radioisotopes; D010329:Patella; D011392:Proline / Q000097:blood / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013710:Tendons / Q000378:metabolism* / Q000502:physiology; D013848:Thigh; D014633:Valine / Q000097:blood
D002250:Carbon Radioisotopes; D045782:Hemiterpenes; D007651:Keto Acids; D009124:Muscle Proteins; D009590:Nitrogen Radioisotopes; C001505:alpha-ketoisovalerate; C013082:alpha-ketoisocaproic acid; D003094:Collagen; D011392:Proline; D007930:Leucine; D014633:Valine