Setaria digitata: identification and characterization of a hypodermally expressed SXP/RAL2 protein.
SXP-1, an immunodominant filarial protein identified from Wuchereria bancrofti from our centre and previously exploited for diagnosis of human lymphatic filariasis, has been shown to be well conserved across several filarial species. In the present study, we describe the identification of SXP protein from the cattle filarid Setaria digitata using antiserum raised against recombinant WbSXP-1, and were able to detect 34 and 66kDa proteins from the crude protein extracts of S. digitata. These reactive proteins were found to be sheath proteins localized to the hypodermal region of the parasite.
Sasisekhar
B
B
Centre for Biotechnology, Anna University, Chennai, India.
Suba
N
N
Sindhuja
S
S
Sofi
G M A
GM
Narayanan
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D017124:Conserved Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005629:Frozen Sections; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D011817:Rabbits; D017182:Setaria Nematode / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D016056:Immunodominant Epitopes; C079249:SPX-1 protein, Brugia malayi