Isolation and purification of closely related citrus limonoid glucosides by flash chromatography.
Several citrus limonoid glycosides have proved to be particularly difficult to purify using conventional techniques. A reversed-phase flash chromatographic technique has been developed for the separation and isolation of the closely related limonoid glucosides, nomilin 17-beta-D-glucopyranoside and nomilinic acid 17-beta-D-glucopyranoside, with confirmation of their identities by electrospray ionisation mass spectrometry. Furthermore, the semi-purification of the mixture of glucosides enriched with flavanone glucosides such as naringin, narirutin and other limonoid glucosides was obtained. The closely eluting glucosides were successfully separated to achieve a good yield and purity of 93%.
Raman
Girija
G
Texas A&M University, Kingsville Citrus Center, 312 N. International Blvd., Weslaco, TX 78596, USA.
Cho
Minhee
M
Brodbelt
Jennifer S
JS
Patil
Bhimanagouda S
BS
D002845:Chromatography / Q000379:methods; D032083:Citrus paradisi / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D036701:Limonins / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D005960:Glucosides; D036701:Limonins