Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region.
We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T. britovi and T. nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use.
Trichinella National Reference Center, Assistance Publique-Hôpitaux de Paris, Hôpital Cochin, R. Descartes University, 27 rue du Fbrg St. Jacques, 75014 Paris, France.
Aymeric
A
De Bruyne
Hélène
H
Yera
Franck
F
Le Guerhier
Pascal
P
Boireau
Jean
J
Dupouy-Camet
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014234:Trichinella / Q000145:classification* / Q000235:genetics
D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S