In vivo DNA gene electro-transfer: a systematic analysis of different electrical parameters.
BACKGROUND
Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes.


METHODS
In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short- and long-term protein production.


RESULTS
Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys.
Zampaglione
Immacolata
I
Istituto di ricerche di Biologia Molecolare (IRBM), Via Pontina Km 30.600, 00040 Pomezia (Rome), Italy.
Arcuri
Mirko
M
Cappelletti
Manuela
M
Ciliberto
Gennaro
G
Perretta
Gemma
G
Nicosia
Alfredo
A
La Monica
Nicola
N
Fattori
Elena
E
D000818:Animals; D004247:DNA / Q000378:metabolism*; D017097:Electric Impedance; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D008252:Macaca fascicularis; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors
D004247:DNA