[Biochip development for polymorphism analysis in biotransformation system genes].
Large-scale population researches, diagnostics of genetic predisposition to multifactorial diseases, screening of the polymorphic loci associated with individual sensitivity to pharmaceutical preparations, require the development of effective, exact and rapid methods of analysis for detection of many mutations simultaneously. One of the most perspective methods to solve these problems is a method of allele-specific hybridization with biochips. Taking the analysis of mutations in genes CYP1A1, CYP2D6, GSTM1, GSTT1, NAT2, CYP2C9, CYP2C19 and MTHFR as an example we showed the efficiency of using the approach for identification of individual genetic polymorphism. We believe that the biochips can be also a convenient tool in pharmacogenetics researches.
a S
aS
Glotov
T V
TV
Nasedkina
T E
TE
Ivashchenko
R A
RA
Iurasov
S A
SA
Surzhikov
S V
SV
Pan'kov
A V
AV
Chudinov
V S
VS
Baranov
A S
AS
Zasedatelev
D001711:Biotransformation / Q000235:genetics; D004798:Enzymes / Q000235:genetics*; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D010597:Pharmacogenetics / Q000295:instrumentation / Q000379:methods; D011110:Polymorphism, Genetic
D004798:Enzymes