[Detection of small molecules based on surface plasmon (correction of plasman) resonance technology].
OBJECTIVE
To develop a detective method applied in online assaying of astronauts' humours using the portable online bio-molecules analyzer (POBA) based on surface plasmon resonance biosensor.


METHODS
An assay format was developed based on the detection of 2, 4-Dinitrophenyl-hydrazine. The bio-molecule slide was made by DNP-BSA. Range of detection and standard curve were obtained using inhibition assay. Reliability and specificity of the assay were also tested.


RESULTS
1) The linear range of the assay was 7.8 ng/ml-2 micrograms/ml with lower detection limit of 2.5 ng/ml; 2) Preparation of the bio-molecule slide and regeneration of the biosensor ensured detections for many samples.


CONCLUSIONS
This assay method can be used to detect small molecules sensitively, rapidly and easily. It can be repeated with good reliability, and has a good application in space medicine.
Chun-yan
CY
Wang
Jiang-hui
JH
Xiong
Ping
P
Zhong
Tang-bin
TB
Yang
Na
N
Li
Ying-hui
YH
Li
D000337:Aerospace Medicine / Q000295:instrumentation; D018480:Astronauts; D004867:Equipment Design; D006801:Humans; D010659:Phenylhydrazines / Q000032:analysis*; D013026:Space Flight / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine