Immunolocalization of CYP1B1 in normal, human, fetal and adult eyes.
CYP1B1 is a cytochrome P450 enzyme implicated in autosomal recessive primary congenital glaucoma (PCG). The mechanism and function of CYP1B1 in the development of the PCG phenotype is unknown. Previously, investigators have reported detection of Cyp1b1 mRNA in the ciliary body and epithelium and neuroepithelium in the developing mouse eye, employing in situ hybridization techniques. Similarly, additional investigators have detected CYP1B1 mRNA in the iris, ciliary body, non-pigmented ciliary epithelial line, cornea, retinal-pigment epithelium, and retina in the human adult eye, using Northern blotting. This study was designed to immunolocalize CYP1B1 protein in the various ocular structures of normal, human fetal and adult eyes. Normal fetal and adult eyes were immunolabeled with a polyclonal antibody against human CYP1B1 using indirect immunofluorescence, and then compared with appropriate controls. The intensity of immunolabeling of the various ocular structures was assessed by qualitative and semi-quantitative techniques. In the anterior segment anti-CYP1B1 immunoreactivity (IR) was detected early in fetal development in the primitive ciliary epithelium. As well, the most intense CYP1B1 IR was in the non-pigmented ciliary epithelium. In addition, CYP1B1 IR was also present in the corneal epithelium and keratocytes, both layers of the iris pigmented epithelium, and retina. However, CYP1B1 IR was absent in the trabecular meshwork in all of the samples. In general, CYP1B1 immunolabeling in the human fetal eyes was more intense when compared to adult eyes. CYP1B1 IR was primarily immunolocalized to the non-pigmented ciliary epithelium and early in fetal development. In addition, CYP1B1 IR was not detected in the trabecular meshwork. These findings suggest that the abnormalities in the development of the trabecular meshwork in PCG may result from diminished or absent metabolism of important endogenous substrates in the ciliary epithelium due to non-functional CYP1B1 enzyme.
Doshi
Manali
M
Department of Ophthalmology and Visual Sciences, University of Illinois at Chigago, 1855 West Taylor Street, M/C 648, Chicago, IL 60612, USA. mdoshi1@uic.edu
Marcus
Craig
C
Bejjani
Bassem A
BA
Edward
Deepak P
DP
D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D002924:Ciliary Body / Q000737:chemistry / Q000196:embryology; D003315:Cornea / Q000737:chemistry / Q000196:embryology; D065633:Cytochrome P-450 CYP1B1; D005123:Eye / Q000737:chemistry* / Q000196:embryology*; D005260:Female; D047109:Fetal Development; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007498:Iris / Q000737:chemistry / Q000196:embryology; D008297:Male; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D012160:Retina / Q000737:chemistry / Q000196:embryology; D018709:Statistics, Nonparametric; D014129:Trabecular Meshwork / Q000737:chemistry / Q000196:embryology
D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; C585498:Cyp1b1 protein, mouse; D065633:Cytochrome P-450 CYP1B1