Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.
Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.
Paulsen
Ian T
IT
The Institute for Genomic Research, Rockville, Maryland, USA.
Press
Caroline M
CM
Ravel
Jacques
J
Kobayashi
Donald Y
DY
Myers
Garry S A
GS
Mavrodi
Dmitri V
DV
DeBoy
Robert T
RT
Seshadri
Rekha
R
Ren
Qinghu
Q
Madupu
Ramana
R
Dodson
Robert J
RJ
Durkin
A Scott
AS
Brinkac
Lauren M
LM
Daugherty
Sean C
SC
Sullivan
Stephen A
SA
Rosovitz
Mary J
MJ
Gwinn
Michelle L
ML
Zhou
Liwei
L
Schneider
Davd J
DJ
Cartinhour
Samuel W
SW
Nelson
William C
WC
Weidman
Janice
J
Watkins
Kisha
K
Tran
Kevin
K
Khouri
Hoda
H
Pierson
Elizabeth A
EA
Pierson
Leland S
LS
Thomashow
Linda S
LS
Loper
Joyce E
JE
D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D010944:Plants / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000096:biosynthesis / Q000235:genetics
D017262:Siderophores