Discovery of disease-induced post-translational modifications in cardiac contractile proteins.
Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.
Johns Hopkins University School of Medicine, Department of Pediatrics, Division of Cardiology, 720 Rutland Avenue, Ross 1144, Baltimore, MD 21205, USA.
Chao
C
Yuan
Rajashree
R
Ravi
Anne M
AM
Murphy
D000818:Animals; D006331:Heart Diseases / Q000378:metabolism*; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D009124:Muscle Proteins