Interaction of the plasmid-encoded quinolone resistance protein QnrA with Escherichia coli topoisomerase IV.
Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.
Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
John H
JH
Tran
George A
GA
Jacoby
David C
DC
Hooper
D000890:Anti-Infective Agents / Q000494:pharmacology; D027101:DNA Topoisomerase IV / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology
D000890:Anti-Infective Agents; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D015363:Quinolones; D027101:DNA Topoisomerase IV