[Protein profiling of human dendritic cells infected with mycobacterium tuberculosis].
The response of dendritic cells (DCs) plays an essential role in the initiation of immune responses following Mycobacterium tuberculosis (MTB) challenge. Two-dimensional electrophoresis (2-DE) was employed to compare the global protein patterns between human DCs infected and that uninfected with MTB H37Rv ATCC 27294 strains, and 45 protein spots were found to express differentially. Four protein spots which remarkably changed in DCs infected with MTB H37Rv ATCC 27294 strains were measured by matrix assisted laser desorption/ionization tandem time-of-flight (TOF/TOF) mass spectrometry. The data obtained from peptide mass fingerprinting were used in protein database search. Four protein spots in gel were identified as Human Arsenite-stimulated ATPase (hASNA-I), Annexin IV, gamma-actin and Heat shock protein27 (HSP27). These data provide insight into the changed global protein patterns of the DCs after infection and may prove useful for further study in the interaction between MTB and host.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China. cqbjl@163.com
Jia-ling
JL
Bao
Jun
J
Le
Ye-ping
YP
Tian
Yan-ping
YP
Yang
Hong-hai
HH
Wang
D000199:Actins / Q000032:analysis; D017314:Annexin A4 / Q000032:analysis; D053501:Arsenite Transporting ATPases; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D016623:Ion Pumps / Q000032:analysis; D009097:Multienzyme Complexes / Q000032:analysis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000199:Actins; D017314:Annexin A4; D006360:Heat-Shock Proteins; D016623:Ion Pumps; D009097:Multienzyme Complexes; D053501:Arsenite Transporting ATPases