[Tomato R-protein Tm-2(2) gene interacting with ToMV movement protein triggered programmed cell death in tobacco transformants].
Tomato Tm-2(2) and ToMV MP gene are a pair of R-Avr mediated response genes. Tm-2(2) and MP gene expressed in tobacco transformants with single-copy integration respectively. Tm-2(2) transformants displayed virus-resistant specificity as the reaction of original Tm-2(2) tomato species in virus-inoculation test. The results of agro-infiltration experiment and transformant-crossing test between Tm-2(2) plants and MP plants indicated that: (1) Tm-2(2) and ToMV-MP maintained "Gene for Gene" characters in transgenic tobacco; (2) The interaction between Tm-22 and MP of ToMV could induce the programmed cell death in the host tobacco by exo-ethylene stimulation. This result will be useful in furthering understanding of molecular mechanism of Tm-2(2) interaction with ToMV-MP.
Jiang
Guo-yong
GY
Fujian Agricultural and Forestry University, Fuzhou 350002, China. jiangguoyong@hotmail.com
Yang
Ren-cui
RC
D017209:Apoptosis; D015870:Gene Expression; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000821:virology*; D009029:Mosaic Viruses / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D030821:Plants, Genetically Modified / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D010940:Plant Proteins; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins