Characteristics of complement activation in mice bearing Lewis lung carcinomas treated by photodynamic therapy.
Following treatment of Lewis lung carcinomas (LLC) by Photofrin-mediated photodynamic therapy (PDT), tumor tissues and sera of host mice were collected for the analysis of complement activity. Elevated tumor C3 levels were detected between 1 and 24 h after PDT, while serum C3 levels increased significantly at 24 h post therapy. Increased alternative complement pathway activity in the serum was evident between 1 and 3 days post PDT. Blocking C3a- or C5a-receptors in the host mice decreased the efficacy of PDT in producing LLC tumor cures, supporting the importance of complement action in PDT-mediated tumor destruction.
Department of Cancer Imaging, BC Cancer Agency/BC Cancer Research Centre, 601 West 10th Avenue, Vancouver, BC, Canada V5Z 1L3.
Ivana
I
Cecic
Katherine
K
Serrano
Maria
M
Gyongyossy-Issa
Mladen
M
Korbelik
D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000276:immunology*; D003167:Complement Activation / Q000276:immunology*; D003176:Complement C3 / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D003170:Complement Pathway, Alternative; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010778:Photochemotherapy; D011951:Receptors, Complement / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D003176:Complement C3; D011951:Receptors, Complement