Analysis of aristolochic acids and analogues in medicinal plants and their commercial products by HPLC-PAD-ESI/MS.
An HPLC-UV-MS method for the analysis of aristolochic acids A, B, C and D, 7-OH-aristolochic acid A, and aristolic acid in a number of plant materials and their commercial products has been developed. HPLC with photodiode array detection and electrospray ionisation-MS in the selected ion monitoring mode allowed the identification of the target compounds and increased the selectivity of complex analyses such as those associated with multi-botanical preparations. The presented method was used to analyse 10 plant samples and six commercial products that possibly contained aristolochic acids. The resulting chromatographic profiles of the samples were significantly different from each other, and the method was directly transferred to HPLC-MS, which was used to confirm the presence of the six aristolochic acids mentioned above.
Wei
Feng
F
Division of Chinese Materia Medica and Natural Products, National Institute for the Control of Pharmaceutical and Biological Products, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, People's Republic of China. hograwei@hotmail.com
Cheng
Xian-Long
XL
Ma
Lin-Yun
LY
Jin
Wen-Tao
WT
Schaneberg
Brian T
BT
Khan
Ikhlas A
IA
Lin
Rui-Chao
RC
D034341:Aristolochic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal