Evaluation of the cytotoxicity, cytostaticity and genotoxicity of argentatins A and B from Parthenium argentatum (Gray).
Argentatins A and B are abundant triterpenes present in Parthenium argentatum. Both compounds have shown cytotoxic properties on K562, MCF-7, PC-3, HCT-15 and U251 human cancer cell lines. Furthermore the cytotoxic, cytostatic and genotoxic effects of the argentatins on proliferating lymphocytes were evaluated using cytokinesis-block micronucleus test. Argentatin A had no cytostatic properties, but it was cytotoxic for proliferating lymphocytes at a concentration of 25 microM (P < 0.005). On the other hand, argentatin B showed significant cytostatic effects (P < 0.001) at concentrations of 5 to 25 microM and it did not show cytotoxic effects at the same concentrations. Neither argentatin showed genotoxic effects in terms of micronucleus frequency in human lymphocytes. According to these results the argentatins are not able to cause injury on DNA by clastogenic or aneugenic mechanisms.
Parra-Delgado
Hortensia
H
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior, Coyoacán, C. P. 04510, México, D.F.
García-Pillado
Fátima
F
Sordo
Monserrat
M
Ramírez-Apan
Teresa
T
Martínez-Vázquez
Mariano
M
Ostrosky-Wegman
Patricia
P
D000328:Adult; D000704:Analysis of Variance; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D019659:Asteraceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D048750:Cell Nucleus Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048749:Cytokinesis / Q000187:drug effects; D004121:Dimethyl Sulfoxide; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D012235:Rhodamines; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*
D000970:Antineoplastic Agents; D010936:Plant Extracts; D012235:Rhodamines; D014315:Triterpenes; C474473:argentatin B; C465304:argentatin A; C050829:sulforhodamine 101; D004121:Dimethyl Sulfoxide