[The registration of nitric oxide production in mammals using a water-soluble complex of N-methyl-D,L-glucamine dithiocarbamate with Fe3+].
The level of nitric oxide production in the intact rabbit organism was studied using the water-soluble complex of Fe3+ with MGD as a selective spin trap for nitric oxide. The Fe(3+)-MGD3 complex was injected intravenously. It was shown by the EPR method that this injection resulted in the formation of paramagnetic complexes in the urine, as Cu(2+)-MGD2, and nitric oxide spin adducts: nitric oxide-Fe(2+)-MGD2 and nitric oxide-Fe(3+)-MGD2. The level of nitric oxide production was estimated by the ratio of the total amount of these adducts to the nitric oxide-Fe(2+)-MGD2 level, formed after the addition of excessive S-nitrosoglutathione. This value for intact animals was 1.33 +/- 0.13%.
Timoshin
A A
AA
Orlova
Ts R
TsR
Ruuge
E K
EK
Vanin
A F
AF
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005260:Female; D005290:Ferric Compounds / Q000032:analysis*; D009569:Nitric Oxide / Q000097:blood* / Q000652:urine*; D011817:Rabbits; D012680:Sensitivity and Specificity; D019097:Spin Trapping / Q000379:methods; D013859:Thiocarbamates / Q000032:analysis*
D005290:Ferric Compounds; D013859:Thiocarbamates; D009569:Nitric Oxide