Cross protection studies with Eimeria maxima strains.
The purpose of this study was to determine whether differences in fecundity of Eimeria maxima isolates were related to their abilities to elicit cross-protective immunity. Immunizations were initiated by low-dose gavages of sporulated oocysts to day-old broiler chicks under conditions that allowed parasite recycling, and chickens were challenged with homologous and heterologous strains. Immunization efficacies were measured using a protective index calculated from weight gain, gross lesion score, plasma carotenoid, and NO2- + NO3- data. A 4x4 cross- immunization study of four E. maxima strains (designated A-D) showed that strain A, which displayed the lower fecundity, provided no cross-protection against the other three strains. Following several maintenance passages, the fecundity of strain A was increased to that of strain C, and infection with strain A oocysts was able to provide cross-immune protection against challenge with strain C. This study indicates that parasite fecundity is important in providing good immune stimulation, and should be carefully monitored when characterization of the unique immune potentials of Eimeria strains is undertaken.
USDA/ARS, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Bldg. 1040, Rm 103, BARC-East, Beltsville, MD 20705, USA. pallen@ANRI.barc.usda.gov
Patricia C
PC
Allen
Mark C
MC
Jenkins
Katarzyna B
KB
Miska
D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D004539:Eimeria / Q000276:immunology* / Q000472:pathogenicity; D005298:Fertility / Q000276:immunology; D008297:Male; D033661:Oocysts / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D013045:Species Specificity; D014611:Vaccination / Q000379:methods / Q000662:veterinary*