Reduced mobilization of Rev-responsive element-deficient lentiviral vectors.
Infection of cells transduced with a lentiviral vector by human immunodeficiency virus (HIV) could lead to packaging of the lentiviral vector RNA into HIV particles and unintended transfer of the vector. To prevent this, the Rev-responsive element (RRE) of an HIV-1 vector was functionally replaced by a heterologous RNA element (MS2). Providing Rev fused to an MS2 binding protein allowed efficient vector production. Mobilization of the vector from infected target cells was below the level of detection and at least 10(3)- to 10(4)-fold lower than for the RRE-containing vector. Thus, RRE-deficient lentiviral vectors provide a novel approach to reduce the risk of vector mobilization.
Lucke
Susann
S
Department of Molecular and Medical Virology, Ruhr University Bochum, D-44780 Bochum, Germany.
Grunwald
Thomas
T
Uberla
Klaus
K
D015694:Gene Products, rev / Q000502:physiology*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D020218:Response Elements / Q000502:physiology*; D014162:Transfection; D019065:Virus Assembly; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D054321:rev Gene Products, Human Immunodeficiency Virus