Fructosyl-amino acid oxidases of Aspergillus oryzae are induced by the reaction product, glucosone.
Aspergillus oryzae has two fructosyl-amino acid oxidase (FAOD) isozymes (AoFao1 and AoFao2), which are different in the substrate specificities. Northern blot analysis showed both FAO genes were induced by autoclave-browned medium containing l-lysine or l-valine. Studies with a mutant, that had a disrupted AoFAO2 gene, revealed that the expression of AoFAO1 by fructosyl l-valine depended on the expression of AoFAO2. Both genes were also induced by one of the FAOD-reaction products, glucosone. In contrast, other alpha-dicarbonyl compounds, which display a similar structure to that of glucosone were not able to induce the genes expression. These results imply that glucosone may contribute to the expression of FAO genes.
Yoshida
Nobuyuki
N
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp
Akazawa
Shin-ichi
S
Kuwahara
Ayako
A
Katsuragi
Tohoru
T
Tani
Yoshiki
Y
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005800:Genes, Fungal / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007661:Ketoses / Q000378:metabolism*; D008239:Lysine; D013379:Substrate Specificity; D014633:Valine
D003470:Culture Media; D007527:Isoenzymes; D007661:Ketoses; C017022:glucosone; D000594:Amino Acid Oxidoreductases; C103012:amadoriase; D014633:Valine; D008239:Lysine