Lipopolysaccharide binding protein-deficient mice have a normal defense against pulmonary mycobacterial infection.
Lipopolysaccharide (LPS) binding protein (LBP) facilitates the transfer of LPS of Gram-negative bacteria to the pattern recognition receptor CD14, resulting in activation of immunocompetent cells. LBP can also facilitate the binding of lipoarabinomannan, a major cell wall component of mycobacteria, to immune cells. To determine the role of LBP in the immune response to pulmonary Mycobacterium tuberculosis infection, LBP gene-deficient (-/-) and normal wild-type (WT) mice were intranasally infected with M. tuberculosis. LBP-/- mice displayed a similar survival and mycobacterial outgrowth in lungs and liver, although they demonstrated a reduced lymphocyte recruitment and activation during the early stages of infection. The clearance of pulmonary infection with the non-pathogenic M. smegmatis was also unaltered in LBP-/- mice. These data suggest that LBP does not contribute to an effective host response in M. tuberculosis infection.
Department of Experimental Internal Medicine, Academic Medical Center, Room F4-222, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. J.Branger@amc.uva.nl
Judith
J
Branger
Jaklien C
JC
Leemans
Sandrine
S
Florquin
Peter
P
Speelman
Douglas T
DT
Golenbock
Tom
T
van der Poll
D000209:Acute-Phase Proteins / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D002452:Cell Count; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000473:pathology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009165:Mycobacterium Infections, Nontuberculous / Q000276:immunology / Q000382:microbiology; D020102:Mycobacterium smegmatis / Q000276:immunology; D016019:Survival Analysis; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D018925:Chemokines; D016207:Cytokines; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein