A modified analytical method for total antioxidant potential assay using RP-HPLC with electrochemical detection and its application for pro- and antioxidative properties of dopamine measurement.
Total antioxidant potential (TAP) is usually measured using photometric or fluorometric assays. Preliminary results of a reversed-phase high-performance liquid chromatography--electrochemical detection assay are given. The method is based on the generation of hydroxyl radicals in a Fenton reaction and analysis of the product of their interaction with p-hydroxybenzoic acid (3,4-dihydroxybenzoic acid). The method is applied to estimate the TAP of dopamine. As a result, depending on the concentration, dopamine is pro- or antioxidant. The results are compared with TAP measurements using a standard photometric method.
Glód
Bronislaw K
BK
Meat and Fat Research Institute, Jubilerska 4, 04-190 Warsaw, Poland. bkg@cmdik.pan.pl
Stanczak
Kinga I
KI
Wozniak
Anna
A
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D017665:Hydroxyl Radical / Q000032:analysis / Q000737:chemistry; D010226:Parabens / Q000737:chemistry; D010783:Photometry
D000975:Antioxidants; D010226:Parabens; D017665:Hydroxyl Radical; C038193:4-hydroxybenzoic acid; D004298:Dopamine