[Computational search for potential post-translational modification sites in human RNA polymerase III subunits].
The transcription of small stable non-translated RNA genes (class III genes), directed by RNA polymerase III, is strictly regulated in accordance to physiological state of the cell (growth rate, cell cycle stage, apoptosis, etc.) Post-translational modifications of the polymerase may play the important role in class III gene transcription regulation. Using computational programs searching for potential post-translational modifications sites in proteins (MotifScan, NetPhos 2.0, and Yin-Yan 1.2), possible sites of phosphorylation were identified in all 17 subunits of human RNA polymerase III, and possible sites of reciprocal phosphorylation and glycosilation ("yin-yan" sites) - in 13 subunits. Among the identified sites -17 sites of phosphorylation in seven subunits are conservative in human, Saccharomyces cerevisiae and Schizosaccharomyces pombe, including two "yin-yan" sites in two subunits. The data obtained can be used for experimental identification of RNA polymerase III modification sites in vivo in cells being in different physiological states.
Nikitina
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Tishchenko
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D019295:Computational Biology / Q000379:methods; D006801:Humans; D046911:Macromolecular Substances; D046912:Multiprotein Complexes / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D012320:RNA Polymerase III / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods; D012984:Software
D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D012320:RNA Polymerase III