Human embryonic stem cells as a model for nutritional programming: an evaluation.
Our laboratory is evaluating whether an epigenetic mechanism involving alterations in DNA methylation can alter the trajectory of embryonic/fetal development in response to maternal nutrients. A similar mechanism may operate in embryo culture environments commonly used in human assisted conception. Since developmental studies on early human embryos in utero are obviously not possible, we have begun to investigate the utility of human embryonic stem cells (hESC) to uncover potential programming mechanisms. This review highlights some of the advantages and problems associated with such a model and suggests that these issues are also broadly applicable to utility of hESC for more general toxicology and drug screening applications.
University of Nottingham, Obstetrics and Gynaecology, D Floor East Block, Queens Medical, Nottingham NG7 2UH, UK.
Cinzia
C
Allegrucci
Chris N
CN
Denning
Paul
P
Burridge
William
W
Steele
Kevin D
KD
Sinclair
Lorraine E
LE
Young
D000328:Adult; D019175:DNA Methylation; D047108:Embryonic Development / Q000502:physiology*; D044127:Epigenesis, Genetic / Q000502:physiology*; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D006801:Humans; D008954:Models, Biological; D039904:Pluripotent Stem Cells / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects