[Construction of efficient conjugal plasmids between Escherichia coli and Streptomycetes].
Conjugal plasmid pGH112 has been developed based on the replicons of Streptomyces coelicolor plasmid SCP2 and E. coli ColE. The plasmid contains ampicilin resistance gene(amp) for selection in E. coli and thiostrepton resistance gene (tsr) for selection in Streptomycetes, and a 0.76 kb oriT fragment of (IncP) RK2. Conjugal transfer of pGH112 was performed from E. coli to S. coelicolor A3(2), S. avermitilis, S. lividans TK54, S. toxytricini NNRL15443, S. venezuelae ISP5230 and Sacc. erythraea by conjugation, results show that the plasmid was able to transfer efficenctly from E. coli to Streptomycetes, was stably inherited in the recipients. pGH113 was constructed from pGH112 by combining the constitutive ermE promoter with green fluorescent protein gene(gfp).
Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, China.
Hong-Bo
HB
Mo
Lin-Quan
LQ
Bai
Sheng-Lan
SL
Wang
Ke-Qian
KQ
Yang
D000668:Ampicillin Resistance / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D010957:Plasmids; D013306:Streptomycetaceae / Q000235:genetics*; D013883:Thiostrepton / Q000494:pharmacology
D049452:Green Fluorescent Proteins; D013883:Thiostrepton