Tissue microarrays: bridging the gap between research and the clinic.
Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced to a tissue microarray format and are being applied to preclinical drug development. In clinical research, tissue microarrays are applied at multiple levels: comprehensive analysis of samples in the context of a clinical trial or across a population. Tissue microarrays play a central role in translational research, facilitating the discovery of molecules that have potential roles in the diagnosis, prognosis and prediction of response to therapy.
Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4605, USA. braunsct@mail.nih.gov
Till
T
Braunschweig
Joon-Yong
JY
Chung
Stephen M
SM
Hewitt
D000818:Animals; D002460:Cell Line; D002986:Clinical Trials as Topic; D006801:Humans; D017403:In Situ Hybridization; D046228:Microarray Analysis / Q000379:methods*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D012107:Research Design
D012313:RNA