Purification of astilbin and isoastilbin in the extract of smilax glabra rhizome by high-speed counter-current chromatography.
Purification of astilbin from the rhizome extract of Smilax glabra was conducted using a high-speed counter-current chromatograph equipped with a 700 mL column. In a single operation, 1.5 g of crude sample was separated to yield 105 mg of component astilbin and 48 mg of isoastilbin while the upper phase of the two-phase solvent system composed of n-hexane-n-butanol-water (1:1:2, v/v/v) was used for stationary phase. The chemical structures of the two flavonoid glycosides were confirmed by electrospray ionization ion trap multiple mass spectrometry and nuclear magnetic resonance experiments.
Institute of Food and Biological Engineering, Zhemjiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang 310035, China. qizhendu@mail.zjgsu.edu.cn
Qizhen
Q
Du
Lei
L
Li
Gerold
G
Jerz
D003377:Countercurrent Distribution / Q000379:methods*; D044948:Flavonols / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*; D032281:Smilax / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D044948:Flavonols; D010936:Plant Extracts; C099069:astilbin