A single active-site region for a group II intron.
Despite the biological importance of self-splicing group II introns, little is known about their structural organization. Synthetic incorporation of site-specific photo-cross-linkers within catalytic domains resulted in functional distance constraints that, when combined with known tertiary interactions, provide a three-dimensional view of the active intron architecture. All functionalities important for both steps of splicing are proximal before the first step, suggestive of a single active-site region for group II intron catalysis.
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th St., New York, New York 10032, USA.
Alexandre
A
de Lencastre
Stephanie
S
Hamill
Anna Marie
AM
Pyle
D001665:Binding Sites / Q000235:genetics / Q000502:physiology*; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives; D007438:Introns / Q000235:genetics / Q000502:physiology*; D008958:Models, Molecular; D012326:RNA Splicing / Q000235:genetics / Q000502:physiology*; D013872:Thionucleosides; D013891:Thiouridine
D003432:Cross-Linking Reagents; D013872:Thionucleosides; D006151:Guanosine; D013891:Thiouridine; C005783:6-thioguanosine