Describing RNA structure by libraries of clustered nucleotide doublets.
The rapidly increasing wealth of structural information on RNA and knowledge of its varying roles in biology have facilitated the study of RNA structure using computational methods. Here, we present a new method to describe RNA structure based on nucleotide doublets, where a doublet is any two nucleotides in a structure. We restrict our search to doublets that are close together in space, but not necessarily in sequence, and obtain doublet libraries of various sizes by clustering a large set of doublets taken from a data set of high-resolution RNA structures. We demonstrate that these libraries are able to both capture structural features present in RNA and fit local RNA structure with a high level of accuracy. Libraries ranging in size from ten to 100 doublets are examined, and a detailed analysis shows that a library with as few as 30 doublets is sufficient to capture the most common structural features, while larger libraries would be more appropriate for accurate modeling. We anticipate many uses for these libraries, from annotation to structure refinement and prediction.
Department of Structural Biology, Stanford University School of Medicine, D100 Fairchild Building, Stanford, CA 94305 USA. sykes@stanford.edu
Michael T
MT
Sykes
Michael
M
Levitt
D016000:Cluster Analysis; D018900:Dinucleotide Repeats; D015723:Gene Library; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA