An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replication in vitro.
OBJECTIVE
To explore the activities of Composite Artemisia Capillaris Tablet (CACT) against hepatitis B virus replication in vitro.


METHODS
By means of radioimmunoassay (RIA), Dot blot and Southern blot, the surface and e antigen production of 2.2.15 cells, HBV DNA in 2.2.15 cell culture medium and that in 2.2.15 cells were examined respectively.


RESULTS
HBsAg, HBeAg values of 2.2.15 cells treated by CACT were lower than those of the control, the HBV DNA quantities in culture medium and in 2.2.15 cells decreased as compared with those cells with no treatment by CACT given to them.


CONCLUSIONS
CACT could inhibit HBV DNA replication, showing its potential antiviral activity in hepatitis B treatment.
Department of Pharmacy, 302 Hospital of PLA, Beijing, China.
Jin
J
Han
Yan-ling
YL
Zhao
Li-mei
LM
Shan
Feng-jiao
FJ
Huang
Xiao-he
XH
Xiao
D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000037:antagonists & inhibitors; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008516:Medicine, Chinese Traditional; D028321:Plant Preparations / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D011863:Radioimmunoassay; D013607:Tablets; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D028321:Plant Preparations; D013607:Tablets