[Study on the expression of IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis].
OBJECTIVE
To investigate the cell type which express IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis.


METHODS
12 patients with chronic periodontitis who were undergoing flap operation were randomly chosen as periodontitis group;10 patients undergoing gingivectomy were chosen as gingivitis group;6 patients undergoing extraction of impacted teeth were chosen as control group. Assays of in situ hybridization and immunohistochemistry were used to observe the expression of IL-10 mRNA and the protein in gingival tissues of each group respectively . Wilcoxon test was used to compare the expression of IL-10 of each group at two levels; differences between the two groups were analyzed by one-way ANOVA test.


RESULTS
IL-10 mRNA and the protein were all expressed in gingival tissues of periodontium,they were found in lymphocytes, fibroblasts and macrophages. There were no significant differences between two levels of IL-10 expression of periodontitis group and control group (P>0.05) gingivitis group,P<0.05). The IL-10 expression of periodontitis group was significantly higher than gingivitis group and control group (P<0.01); The IL-10 mRNA expression of periodontitis group was significantly higher than control group (P<0.01),but there was no significant difference between the IL-10 mRNA expression of periodontitis group and gingivitis group (P>0.05).


CONCLUSIONS
There is a local secretion mechanism of IL-10 in the periodontal tissues.
Department of Oral Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
Pei-hong
PH
Cheng
Xiang-min
XM
Qi
Shan-zhen
SZ
Sun
Lin
L
Liu
D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism*; D005881:Gingiva; D005891:Gingivitis / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D012333:RNA, Messenger
D012333:RNA, Messenger; D016753:Interleukin-10