Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis.
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
Fundación Ciencia para la Vida, Santiago, Chile.
Vivian
V
Wilhelm
Cristián
C
Soza
Rodrigo
R
Martínez
Mario
M
Rosemblatt
Luis O
LO
Burzio
Pablo D T
PD
Valenzuela
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001483:Base Sequence; D018834:Chaperonin 60 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics*; D015698:Genomic Library; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D044147:Piscirickettsiaceae / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012474:Salmon / Q000276:immunology / Q000382:microbiology*
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D004269:DNA, Bacterial; D018840:HSP70 Heat-Shock Proteins