Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.
A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 16502 Praha 6, Czech Republic. dobrev@ueb.cas.cz
P I
PI
Dobrev
L
L
Havlícek
M
M
Vagner
J
J
Malbeck
M
M
Kamínek
D000040:Abscisic Acid / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007210:Indoleacetic Acids / Q000032:analysis / Q000302:isolation & purification*; D010937:Plant Growth Regulators / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D000040:Abscisic Acid