[Chemical diagnosis of carbamazepine intoxication and its usefulness in toxicological jurisprudence].
Intoxication with carbamazepine (Amizepine, Tegretol) represents a significant problem of clinical and forensic toxicology which frequently develops independently of the commonly used monitored therapy. In the study a technique of qualitative and quantitative analysis applied to carbamazepine (CBZ) as well as its metabolites carbamazepine 10, 11-epoxide (CBZ-E) and 10, 11-dihydroxycarbamazepine (CBZ-DH) in biological material using high-performance liquid chromatography (HPLC) was presented. The studies also involve isolation of studied compounds using solid phase extraction, SPE-Abselut NEXUS (Varian), establishment of calibration range and validation of analytical parameters. This was followed by a discussion of results obtained in routine diagnostic investigations in cases of intoxications, corroborating practical suitability of the designed technique.
Katedry i Zakładu Medycyny Sadowej, Akademii Medycznej im. K. Marcinkowskiego, Poznań.
Roman
R
Wachowiak
Jarosław
J
Tobolski
Dorota
D
Klimaszyk
D000927:Anticonvulsants / Q000009:adverse effects / Q000378:metabolism; D018692:Antimanic Agents / Q000009:adverse effects / Q000378:metabolism; D002220:Carbamazepine / Q000008:administration & dosage / Q000009:adverse effects* / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D011041:Poisoning / Q000175:diagnosis / Q000209:etiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*; D014116:Toxicology / Q000331:legislation & jurisprudence
D000927:Anticonvulsants; D018692:Antimanic Agents; D002220:Carbamazepine