Photoresponsive flagellum-independent motility of the purple phototrophic bacterium Rhodobacter capsulatus.
We report the discovery of photoresponsive, flagellum-independent motility of the alpha-proteobacterium Rhodobacter capsulatus, a nonsulfur purple phototrophic bacterium. This motility takes place in the 1.5% agar-glass interface of petri plates but not in soft agar, and cells move toward a light source. The appearances of motility assay plates inoculated with wild-type or flagellum-deficient mutants indicate differential contributions from flagellar and flagellum-independent mechanisms. Electron microscopy confirmed the absence of flagella in flagellar mutants and revealed the presence of pilus-like structures at one pole of wild-type and mutant cells. We suggest that R. capsulatus utilizes a flagellum-independent, photoresponsive mechanism that resembles twitching motility to move in a line away from the point of inoculation toward a light source.
Shelswell
Kristopher J
KJ
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada.
Taylor
Terumi A
TA
Beatty
J Thomas
JT
D002874:Chromosome Mapping; D005407:Flagella / Q000502:physiology* / Q000648:ultrastructure; D009068:Movement / Q000502:physiology; D009154:Mutation; D016366:Open Reading Frames; D018524:Phototropism / Q000502:physiology*; D016354:Rhodobacter capsulatus / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure