Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol.
Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 mug GTP ml(-1) significantly (p<0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.
Han
Dong-Wook
DW
Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea.
Kim
Hak Hee
HH
Lee
Mi Hee
MH
Baek
Hyun Sook
HS
Lee
Kwon-Yong
KY
Hyon
Suong-Hyu
SH
Park
Jong-Chul
JC
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D005615:Freezing; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D013662:Tea
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013662:Tea; D006160:Guanosine Triphosphate; D000469:Alkaline Phosphatase; D004121:Dimethyl Sulfoxide