[Cloning of alpha-tubulin gene from Fusarium graminearum and analyzing its relationship with carbendazim-resistance].
The full-length nucleotide sequence of alpha-tubulin gene from each of 6 Fusarium graminearum strains from China which had different carbendazim (MBC) sensitivity phenotypes were separated using PCR with 4 primer sets designed in accordance with nucleotide sequence of the gene from the reference isolate, NRRL 31084 (PH-1). The DNA sequence comparison showed that there was no difference in the nucleotide sequence of alpha-tubulin gene amongst 3 sensitive and 3 resistant strains from China. This result demonstrates that there is no relationship between MBC-resistance and alpha-tubulin gene. The full-length of the gene spans 1718 bp, including 6 introns, encoding 449 amino acids. With 99% homology, there is 5 nucleotide differences in alpha-tubulin gene between PH-1 isolate and the 6 strains from China. The homology of the deduced amino acid sequence of the gene is 99.78% between the 6 strains and PH-1 isolate, and 37%-86% between the 6 strains and other 6 species of fungi.
Chen
Chang-jun
CJ
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. ccj100cn@1613.com
Li
Jun
J
Qi
Zhi-qiu
ZQ
Wang
Jian-xin
JX
Zhou
Ming-guo
MG
D000595:Amino Acid Sequence; D001483:Base Sequence; D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D003001:Cloning, Molecular; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics; D005800:Genes, Fungal; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014404:Tubulin / Q000737:chemistry / Q000235:genetics*
D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; D014404:Tubulin; C006698:carbendazim