Use of Fourier-transform infrared spectroscopy to rapidly diagnose gastric endoscopic biopsies.
OBJECTIVE
To determine if Fourier-transform infrared (FT-IR) spectroscopy of endoscopic biopsies could accurately diagnose gastritis and malignancy.


METHODS
A total of 123 gastroscopic samples, including 11 cases of cancerous tissues, 63 cases of chronic atrophic gastritis tissues, 47 cases of chronic superficial gastritis tissues and 2 cases of normal tissues, were obtained from the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance (ATR) accessory was linked to a WQD-500 FT-IR spectrometer for spectral measurement followed by submission of the samples for pathologic analysis. The spectral characteristics for different types of gastroscopic tissues were summarized and correlated with the corresponding pathologic results.


RESULTS
Distinct differences were observed in the FT-IR spectra of normal, atrophic gastritis, superficial gastritis and malignant gastric tissues. The sensitivity of FT-IR for detection of gastric cancer, chronic atrophic gastritis and superficial gastritis was 90.9%, 82.5%, 91.5%, and specificity was 97.3%, 91.7%, 89.5% respectively.


CONCLUSIONS
FT-IR spectroscopy can distinguish gastric inflammation from malignancy.
Li
Qing-Bo
QB
State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Sun
Xue-Jun
XJ
Xu
Yi-Zhuang
YZ
Yang
Li-Min
LM
Zhang
Yuan-Fu
YF
Weng
Shi-Fu
SF
Shi
Jing-Sen
JS
Wu
Jin-Guang
JG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D005260:Female; D005756:Gastritis / Q000175:diagnosis* / Q000473:pathology; D005773:Gastroscopy; D006801:Humans; D008297:Male; D008875:Middle Aged; D017550:Spectroscopy, Fourier Transform Infrared / Q000592:standards; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000473:pathology; D013997:Time Factors