[Analysis of sequence-tagged site in bcr and abl genes by DNA pooling and dHPLC].
To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people.
Department of Hematology, Fuzhou General Hospital of Nanjing Military Area, Fuzhou 350025, China. tianh710@hotmail.com
Hong
H
Tian
Dao-Ming
DM
Liu
Bing
B
Xu
Wei-Yang
WY
Zheng
Shu-Yun
SY
Zhou
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites
D016044:Fusion Proteins, bcr-abl; D051562:Proto-Oncogene Proteins c-bcr