[Microbial community composition and phylogenetic analysis of deodor biofilter under different pH conditions].
Molecular biological methods were applied to analyze the genetic diversity of microbe community in biofilter. V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. Then PCR-DGGE combined technique were used to analyze the microbial population composition and phylogeny of deodorant biofilter. The results indicate that there are prominent differences in microbial diversity and abundance between acid and neutral conditions. Owing to the selective pressure of extreme acidity, there is less diversity of microbe compared with that in neutral environment. It is demonstrated the obvious distinction of spacial diversity in different biofilter bed as well. PCR and T-vector cloning sequencing results shows that the sulfur-oxidizing bacteria are the predominant population. The research offers the valid scientific basis for better treatment of odorant gas and the theoretical foundation for application of bio-deodorization.
Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China. sand-cts@yahoo.com.cn
Tong-sheng
TS
Chen
Jian-jun
JJ
Li
Ying-hua
YH
Cen
Guo-ping
GP
Sun
D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D005374:Filtration; D014644:Genetic Variation; D006862:Hydrogen Sulfide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006862:Hydrogen Sulfide