Co-expression of radial glial marker in macrophages/microglia in rat spinal cord contusion injury model.
Macrophages/microglia are implicated in spinal cord injury but their precise role in the process is not clear. Our previous studies have reported that radial glia (RG) possess properties of neural stem cells and remerged after central nervous system (CNS) injury which may play an important role in neural repair and regeneration. In the present study, we examined the expression of ED1 (a specific marker for activated macrophages/microglia) and RG in a spinal cord injury (SCI) model and detected the activation at 1, 4, 8, and 12 weeks in both dorsal funiculus and ventral white matter after SCI. For both ED1-positive cells and RG cells, there was a gradual increase in density and in number from 1 to 4 weeks followed by down-regulation up to 12 weeks after injury. The morphologies of macrophages and radial glia were different. However, some ED1-positive cells were also stained by RG marker. These results suggest that macrophages may have some lineage to radial glial cells.
Department of Orthopaedic Surgery, School of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
Di
D
Wu
Osamu
O
Miyamoto
Sei
S
Shibuya
Satoshi
S
Mori
Hiromichi
H
Norimatsu
Najma A
NA
Janjua
Toshifumi
T
Itano
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D053331:Ectodysplasins; D007150:Immunohistochemistry; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D017628:Microglia / Q000166:cytology* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D015415:Biomarkers; D053331:Ectodysplasins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins