Luteolin induces vasorelaxion in rat thoracic aorta via calcium and potassium channels.
The aims of the present study were to investigate the vasoactive effects of luteolin and its mechanisms of action on the rat thoracic aorta. Luteolin (4.5-36 micromol/L) caused a concentration-dependent relaxation of endothelium-intact or endothelium-denuded aortic rings precontracted with phenylephrine (PE, 10(-6) mol/L) or a high level of K+ (6 x 10(-2) mol/L). Luteolin induced a shift of the PE concentration-response curve to the right and downward. L-NAME and propranolol did not influence the vascular effect of luteolin. However, 5-hydroxydecanoate, tetraethylammonium, BaCl2 and 4-aminopyridine significantly attenuated the vasorelaxant effect of luteolin. In Ca2+ -free medium, medium with graded concentrations of Ca2+, or K+ -free solution, luteolin reduced PE-induced contraction. It is concluded that luteolin induces endothelium-independent relaxation in rat thoracic aorta. The mechanism involves the inhibition of sarcolemmal Ca2+ channels, release from intracellular Ca2+ stores and activation of K+ channels.
Jiang
Huidi
H
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China. hdjiang@zju.edu.cn
Xia
Qiang
Q
Wang
Xiaoxia
X
Song
Jianfeng
J
Bruce
I C
IC
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000502:physiology; D066298:In Vitro Techniques; D047311:Luteolin / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010656:Phenylephrine / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D000319:Adrenergic beta-Antagonists; D015220:Calcium Channels; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D011433:Propranolol; D047311:Luteolin