Glucocorticoids remodel nuclear envelope structure and permeability.
The present study describes glucocorticoid induced remodelling of nuclear envelope (NE) structure and permeability. A glucocorticoid analogue, triamcinolone acetonide (TA), is injected into Xenopus laevis oocytes that express an exogeneous glucocorticoid receptor (GR). Electrical, fluorescence and nano-imaging techniques are applied to study the permeability and the structure of the NE at 5 and 60 minutes after injection of TA. A remarkable dilation of nuclear pore complexes (NPCs), a rearrangement of NPC distribution and a significant increase of NE permeability for ions and fluorescent 20 kDa dextran are observed within 5 minutes of TA exposure. At regular distances on local NE patches, NPCs seem to adjoin forming clusters each consisting of several hundred NPCs. Interestingly, at the same time of exposure, hydrophobicity of NPC central channels and NPC-free NE surface increases. The changes in permeability and structure are transient as the NE permeability returns to its initial state within 60 minutes. In conclusion, the NE is a barrier of high plasticity sensitive to hydrophobic molecules. Remodelling of NE structure and permeability is a prerequisite for mediating physiological actions of glucocorticoids.
Shahin
Victor
V
Institute of Physiology II, University of Münster, Robert-Koch Str. 27b, 48149 Münster, Germany. shahin@uni-muenster.de
Ludwig
Yvonne
Y
Schafer
Claudia
C
Nikova
Dessy
D
Oberleithner
Hans
H
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D003911:Dextrans / Q000737:chemistry; D004553:Electric Conductivity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005938:Glucocorticoids / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D009685:Nuclear Envelope / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties; D014222:Triamcinolone Acetonide / Q000494:pharmacology*; D014982:Xenopus laevis
D003911:Dextrans; D005938:Glucocorticoids; D014222:Triamcinolone Acetonide; D016650:Fluorescein-5-isothiocyanate