A universal BMV-based RNA recombination system--how to search for general rules in RNA recombination.
At present, there is no doubt that RNA recombination is one of the major factors responsible for the generation of new RNA viruses and retroviruses. Numerous experimental systems have been created to investigate this complex phenomenon. Consequently, specific RNA structural motifs mediating recombination have been identified in several viruses. Unfortunately, up till now a unified model of genetic RNA recombination has not been formulated, mainly due to difficulties with the direct comparison of data obtained for different RNA-based viruses. To solve this problem, we have attempted to construct a universal system in which the recombination activity of various RNA sequences could be tested. To this end, we have used brome mosaic virus, a model (+)RNA virus of plants, for which the structural requirements of RNA recombination are well defined. The effectiveness of the new homomolecular system has been proven in an experiment involving two RNA sequences derived from the hepatitis C virus genome. In addition, comparison of the data obtained with the homomolecular system with those generated earlier using the heteromolecular one has provided new evidence that the mechanisms of homologous and non-homologous recombination are different and depend on the virus' mode of replication.
Alejska
Magdalena
M
Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14, 61-704 Poznań, Poland.
Figlerowicz
Magdalena
M
Malinowska
Nelli
N
Urbanowicz
Anna
A
Figlerowicz
Marek
M
D017795:Bromovirus / Q000235:genetics*; D005822:Genetic Vectors; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D011995:Recombination, Genetic
D012367:RNA, Viral