Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS.
BACKGROUND
HIV-infected adults are highly susceptible to pneumococcal disease.


OBJECTIVE
To examine if alveolar macrophages from HIV-infected subjects exhibited a failure of cytokine production in response to Streptococcus pneumoniae in vitro.


METHODS
Case-control comparison of alveolar macrophages from 11 HIV-infected and 13 non-infected adults.


METHODS
Type 1 opsonized S. pneumoniae were used to challenge the alveolar macrophages in vitro. Cell supernatant fluid was collected from unstimulated cells, and cells challenged with bacteria for 0, 6, 12 and 24 h. Cytokine production (interleukins 1beta, 6 and 8) was measured in all fluids using an enzyme-linked immunosorbent assay.


RESULTS
All the cytokines tested increased over time in both HIV-infected and uninfected subjects. Interleukin-8 release was significantly lower in HIV-infected than in non-HIV-infected subjects (P = 0.02).


CONCLUSIONS
Reduced interleukin-8 production may result in decreased neutrophil recruitment, and hence increased susceptibility to pneumococcal infection in HIV-infected subjects.
Gordon
Stephen B
SB
Malawi-Liverpool-Wellcome Trust Research Programme, Universities of Malawi and Liverpool, Malawi. sbgordon@liverpool.ac.uk
Jarman
Elizabeth R
ER
Kanyanda
Stonard
S
French
Neil
N
Pridmore
Alison C
AC
Zijlstra
Eduard E
EE
Molyneux
Malcolm E
ME
Read
Robert C
RC
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D016676:Macrophages, Alveolar / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D011008:Pneumococcal Infections / Q000378:metabolism* / Q000821:virology; D013296:Streptococcus pneumoniae
D016209:Interleukin-8