[Construction of infectious clone of subgroup J avian leukosis virus strain NX0101 and its pathogenicity].
By using PCR, 3 fragments of provirus cDNA of avian leukosis virus (ALV-J) strain NXO101 were amplified from the genomic DNA of ALV-J infected cells,and then combined in the right direction and sequences into recombinant plasmid pALV-J-NX, containing the whole genome of NX0101. After transfection of chicken embryo fibroblast (CEF) cells with plasmid pALV-J-NX DNA, the rescued virus was identified in CEF by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The rescued virus could replicate in CEF at a titer of 10(5.6)/mL. The chicken experiment demonstrated that the rescued virus was still able to induce tumors in commercial meat-type broilers.
Zhang
Ji-yuan
JY
College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China. uniquezjy@163.com
Cui
Zhi-zhong
ZZ
Ding
Jia-bo
JB
Jiang
Shi-jin
SJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000472:pathogenicity*; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014162:Transfection
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D004279:DNA, Viral