[RAPD Identification of Three Types of Three-lines Hybrid Rice with Defferent Male Sterile Cytoplasms and Their Parent Lines.].
The RAPD analysis was conducted on genome DNA from 21 sets of rice, including 6 three-line hybrid rice combinations, separately derived from three different kinds of cytoplasmic male sterile (CMS) lines and their related parents. Out of 264 random primers screened first, 25 primers displayed well in polymorphisms. It was shown that only 7 bands amplified respectively from 7 primers were enough to discriminate the different types of CMS, the hybrid combinations and their parents.
Biotechnological Laboratory of National Commission for Nationalities, Colloge of Chemistry and Life Science, South-central University for Nationalities, Wuhan 430074, China.
Xing
X
Xu
Xue-Qun
XQ
Liu
Bo
B
Qu
Ying-Guo
YG
Zhu
Chun-Tai
CT
Wang
D003593:Cytoplasm / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics; D010290:Parents; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D018744:DNA, Plant