Alcaligenes faecalis kw-a biofilm for denitrification of nitrate-rich effluent.
Alcaligenes faecalis kw-A selected for possessing good denitrification efficiency was used for biofilm development. The biofilm could be developed on a glass surface within 12 hr when 5%, Ix 10(8) cells/ml was used as inoculum. The microcolonies were seen in 6 hr and glycocalyx in 9 hr stage. At 24 hr the biofilm was developed fully and hence was visualised as dense mass. The biofilm protein content showed 48.5% increase in shake flask than in static condition. The exopoplymer is produced in larger amounts in biofilm as compared to the suspended cells. Also, its amount was more by 43% in the biofilm produced in shake flask condition than in static condition. The biofilm could remove 95% nitrate from nitrate-rich effluent in a bench-scale process in 36 hr. The attached growth technique demonstrated here can be utilised to study the effect of favourable as well as adverse conditions on the denitrification efficiency of a culture. The ultimate application of a denitrifying biofilm would be in attached growth or biofilm reactor.
T S
TS
Jadhav
N J
NJ
Faldu
P
P
Patel
S N
SN
Narolkar
A S
AS
Nerurkar
D042481:Alcaligenes faecalis / Q000378:metabolism*; D000818:Animals; D001671:Biochemistry / Q000379:methods; D018441:Biofilms; D002417:Cattle; D005898:Glass; D019276:Glycocalyx / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia; D008853:Microscopy; D008858:Microscopy, Phase-Contrast; D009566:Nitrates / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010461:Peptones / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D009566:Nitrates; D010461:Peptones; D011134:Polysaccharides; D009584:Nitrogen