Protective effect of puerariae radix on oxidative stress induced by hydrogen peroxide and streptozotocin.
This study evaluated the protective effect of Puerariae radix against the oxidative stress induced by hydrogen peroxide (H2O2) and streptozotocin in vitro and in vivo, respectively. The ethanol extract scavenged intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. This radical scavenging activity of the ethanol extract protected the cell viability of Chinese hamster lung fibroblast (V79-4) cells exposed to H2O2. Furthermore, this extract reduced the formation of apoptotic cells induced by H2O2, which was demonstrated by the decreased number of sub G(1) hypo-diploid cells and apoptotic cell body formation. The extract increased the activities of the cellular antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Administration of the extract to the streptozotocin induced diabetic rats decreased the blood glucose levels. The diabetic rats showed low activities of superoxide dismutase and catalase in the liver, and the ethanol extract increased the CAT activity. The increased level of lipid peroxidation in the diabetic rats reverted to near normal levels after being treated with the extract. This study showed that Puerariae radix was effective in the amelioration of diabetes, which may be a consequence of its antioxidant potential.
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Korea.
Kyoung Ah
KA
Kang
Sungwook
S
Chae
Young Sang
YS
Koh
Jin Sook
JS
Kim
Jae-Hwa
JH
Lee
Ho Jin
HJ
You
Jin Won
JW
Hyun
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D013311:Streptozocin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D001786:Blood Glucose; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013311:Streptozocin; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase