Electrochemically assisted microbial production of hydrogen from acetate.
Hydrogen production via bacterial fermentation is currently limited to a maximum of 4 moles of hydrogen per mole of glucose, and under these conditions results in a fermentation end product (acetate; 2 mol/mol glucose) that bacteria are unable to further convert to hydrogen. It is shown here that this biochemical barrier can be circumvented by generating hydrogen gas from acetate using a completely anaerobic microbial fuel cell (MFC). By augmenting the electrochemical potential achieved by bacteria in this MFC with an additional voltage of 250 mV or more, it was possible to produce hydrogen at the cathode directly from the oxidized organic matter. More than 90% of the protons and electrons produced by the bacteria from the oxidation of acetate were recovered as hydrogen gas, with an overall Coulombic efficiency (total recovery of electrons from acetate) of 60-78%. This is equivalent to an overall yield of 2.9 mol H2/mol acetate (assuming 78% Coulombic efficiency and 92% recovery of electrons as hydrogen). This bio-electrochemically assisted microbial system, if combined with hydrogen fermentation that produces 2-3 mol H2/mol glucose, has the potential to produce ca. 8-9 mol H2/mol glucose at an energy cost equivalent to 1.2 mol H2/mol glucose. Production of hydrogen by this anaerobic MFC process is not limited to carbohydrates, as in a fermentation process, as any biodegradable dissolved organic matter can theoretically be used in this process to generate hydrogen from the complete oxidation of organic matter.
Penn State University, University Park, Pennsylvania 16802, USA.
Hong
H
Liu
Stephen
S
Grot
Bruce E
BE
Logan
D000085:Acetates / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D004563:Electrochemistry; D004583:Electrons; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D010084:Oxidation-Reduction; D011522:Protons
D000085:Acetates; D002241:Carbohydrates; D011522:Protons; D006859:Hydrogen