Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.
Several stromal cells were established from murine bone marrow cultures. One of the KUSA subclones, KUSA-A1 cells, displays osteogenic characteristics in vitro and in vivo. The calcium deposition, osteocalcin release, and parathyroid hormone (PTH) responsiveness of KUSA-A1 cells indicate that they are mature osteoblasts or osteocytes. Bone had formed in subcutaneous tissue 1 week after subcutaneous injection of cells into immunodeficient mice. The osteogenesis by KUSA-A1 was not mediated by chondrogenesis and thus was considered to be membranous ossification. These unique characteristics of KUSA-A1 cells provide an opportunity to analyze the process of membranous ossification in detail.
Department of Reproductive Biology and Pathology, National Institute for Child Health and Development, Setagaya-ku, Tokyo 157-8535, Japan.
Satoshi
S
Matsumoto
Isao
I
Shibuya
Satoshi
S
Kusakari
Kaoru
K
Segawa
Taro
T
Uyama
Akinori
A
Shimada
Akihiro
A
Umezawa
D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D002478:Cells, Cultured; D005260:Female; D017629:Gap Junctions / Q000502:physiology; D051379:Mice; D008854:Microscopy, Electron; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism