Involvement of Listeria monocytogenes phosphatidylinositol-specific phospholipase C and host protein kinase C in permeabilization of the macrophage phagosome.
We have previously shown that phosphatidylinositol-specific phospholipase C (PI-PLC) produced by Listeria monocytogenes activates a host protein kinase C (PKC) cascade which promotes escape of the bacterium from a macrophage-like cell phagosome. Here, we provide evidence linking bacterial PI-PLC and host PKC beta to phagosome permeabilization, which precedes escape.
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6076, USA.
Mathilde A
MA
Poussin
Howard
H
Goldfine
D000818:Animals; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins / Q000502:physiology; D006460:Hemolysin Proteins; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000201:enzymology*; D008264:Macrophages / Q000378:metabolism*; D008301:Maleimides / Q000494:pharmacology; D051379:Mice; D010539:Permeability; D010588:Phagosomes / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase / Q000502:physiology*; D054801:Phosphoinositide Phospholipase C; D011493:Protein Kinase C / Q000502:physiology*
D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D007211:Indoles; D008301:Maleimides; C071924:Ro 31-8425; D011493:Protein Kinase C; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; C038705:hlyA protein, Listeria monocytogenes