High-porosity activated carbon as a possible matrix for native DNA and dextran-sulfate immobilization.
In this study, specific and nonspecific activity of calf thymus DNA and Dextran-Sulfate (DS)-containing synthetic carbonic adsorbents (0.7-7mg of each ligands per 1 cm3 of activated carbonic beads, 0.3-0.6mm diameter, bulk density gamma=0.1-0.2g/cm3) have been compared in stir-bath and micro-column in vitro tests. DS coating as well as DNA coating does not demonstrate deep influence on the unspecific adsorptive activity of carbonic matrix toward creatinine, vitamin B12, and unconjugated bilirubin. No essential difference has been found in the specific activity of DNA and DS containing adsorbents toward anti-ds- and anti-ss-DNA-antibodies, as well as antibodies against DNA-protein complexes (anti-DNP-antibodies): in both cases the percentage of decrease of appropriate antibody concentration varied between 35 and 51% for single-pass microcolumn experiments with moderate enhancement of extraction efficacy (up to 60-75%) due to additional recirculation (2 h) or preliminary plasma dilution in 2-5 times. In the micro-column experiments with the proinflammatory cytokines DNA or DS-coating did not diminish TNF-alpha, IL-1beta, and IL-6 adsorption from 3% BSA solution, but even improves to some extent its removal compared with uncoated matrix.
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of Nat. Acad. Sci. of Ukraine, Kiev, Ukraine. aos@onconet.kiev.ua
Elizaveta A
EA
Snezkova
Daniel
D
Muller
Kvitoslava I
KI
Bardakhivskaya
Sergey V
SV
Mikhalovsky
Vladimir G
VG
Nikolaev
D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D016264:Dextran Sulfate / Q000737:chemistry*; D007164:Immunosorbents / Q000737:chemistry*
D007164:Immunosorbents; D002244:Carbon; D004247:DNA; D016264:Dextran Sulfate; C427203:calf thymus DNA