Synaptic localization of receptor-type protein tyrosine phosphatase zeta/beta in the cerebral and hippocampal neurons of adult rats.
Receptor-type protein tyrosine phosphatase (RPTP) zeta/beta is a nervous tissue-specific chondroitin sulfate proteoglycan. In this study, we investigated the immunohistochemical localization of RPTPzeta/beta in adult rat cerebral cortex and hippocampus at light and electron microscopic levels. Double labeling immunofluorescence microscopy revealed that the immunoreactivity of RPTPzeta/beta was observed at MAP2-positive dendrites and PSD-95-positive spines of pyramidal neurons in the cerebral cortex and hippocampus. Electron microscopic observation demonstrated a strong immunoreactivity of RPTPzeta/beta at the postsynaptic membrane of dendritic spines and shafts, and its moderate immunoreactivity at the dendritic membrane. In cultured cortical neurons, the immunoreactivity of RPTPzeta/beta was observed at some of PSD-95-positive spines. These results demonstrate that RPTPzeta/beta is localized mainly at the postsynaptic membrane of pyramidal neurons in adult cerebral cortex and hippocampus.
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
Noriko
N
Hayashi
Atsuhiko
A
Oohira
Seiji
S
Miyata
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D049229:Dendritic Spines / Q000201:enzymology / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D017966:Pyramidal Cells / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D000074407:SAP90-PSD95 Associated Proteins; D013569:Synapses / Q000201:enzymology* / Q000648:ultrastructure
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D000074407:SAP90-PSD95 Associated Proteins; D017027:Protein Tyrosine Phosphatases; C516897:Ptprz1 protein, rat; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5