[Comparison of three methods for evaluating acrosome reaction in human spermatozoa].
OBJECTIVE
To find a convenient and exact method for evaluating acrosome reaction in human spermatozoa.


METHODS
The semen of the normal male was mixed and then divided into 6 groups. Coomassie brilliant blue (CBB) staining, chlortetracycline (CTC) fluorescence staining and acid phosphatase (ACP) detection were used for morphological observation and data analysis of the acrosome status of the human sperm treated with or without progesterone.


RESULTS
There were obvious morphological differences between the acrosome-reaction and acrosome-intact spermatozoa in CBB staining and CTC fluorescence staining, and significant differences were observed between the experimental and control spermatozoa by the three methods (P < 0.05).


CONCLUSIONS
All the three methods can be used to assess acrosome reaction in human spermatozoa, but Coomassie brilliant blue (CBB) staining is much more convenient and stable.
Zhang
Yun
Y
Institute of Reproductive Immunology, Jinan University, Guangzhou, Guangdong 510632, China.
Xie
Qi-Xuan
QX
Pan
Shan-Pei
SP
Zhang
Chun-Xue
CX
Xiao
Luan-Juan
LJ
Peng
Ya-Lin
YL
D000135:Acid Phosphatase; D020101:Acrosome Reaction / Q000187:drug effects; D002478:Cells, Cultured; D002751:Chlortetracycline; D006801:Humans; D008297:Male; D011374:Progesterone / Q000494:pharmacology; D012394:Rosaniline Dyes; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
D012394:Rosaniline Dyes; D011374:Progesterone; C048139:Coomassie blue; D000135:Acid Phosphatase; D002751:Chlortetracycline