Anode glow discharge plasma treatment of titanium plates facilitates adsorption of extracellular matrix proteins to the plates.
Glow discharge plasma (GDP) supplied to an anode (GDP+) promotes calcium phosphate adsorption onto titanium better than that supplied to a cathode (GDP-). However, the adsorption of extracellular matrix (ECM) proteins is crucial for cell adhesion to titanium. Since GDP+ induced both inorganic adsorption and cell adhesion, we hypothesized that the inorganic adsorption in a culture medium might affect the adsorption of the ECM proteins. In this study, ECM proteins adsorbed on titanium with and without GDP were examined by x-ray photoelectron spectroscopy. After 1 hr of incubation, increasing sodium adsorption on GDP+ specimens induced adsorption of ECM proteins as shown by NH(+) and COO(-) signals without calcium adsorption. In contrast, since no specific adsorption of sodium on GDP-specimens was detected, GDP-did not contribute to the adsorption of ECM proteins. Thus, promotion of sodium adsorption of GDP+ was effective, at least in the initial ECM protein adsorption on a titanium surface.
Department of Oral Biomaterials and Technology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. hirokichi@dent.showa-u.ac.jp
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Yamamoto
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Shibata
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Miyazaki
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004577:Electron Probe Microanalysis; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D013499:Surface Properties; D013674:Technology, Dental / Q000379:methods; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D014025:Titanium