Further structural analysis of GnRH complexes with metal ions.
MALDI-TOF mass spectrometry, 1H NMR spectrometry, the continuous variation method and molecular modeling by MM3 calculation confirmed our earlier studies showing that gonadotropin-releasing hormone (GnRH) forms complex with copper(II) ion with the binding ratio 1:1. The copper(II) complex formed at physiological pH has a square planar configuration and GnRH complexes with nickel(II) and cobalt(II) ions are less stable than that of copper(II).
Nakamura
Kazuhiko
K
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. k.kochman@ifzz.pan.pl
Kodaka
Masato
M
El-Mehasseb
Ibrahim M
IM
Gajewska
Alina
A
Okuno
Hiroaki
H
Ochwanowska
Ewa
E
Witek
Bozena
B
Kozłowski
Henryk
H
Kochman
Kazimierz
K
D000818:Animals; D003300:Copper / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019216:Metals, Heavy; D011522:Protons; D007987:Gonadotropin-Releasing Hormone; D003300:Copper; D009532:Nickel