Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA.
Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high-throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as a target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR.
Borucki
Monica K
MK
USDA-ARS, Animal Disease Research Unit, Pullman, WA 99164-6630, USA. mborucki@vetmed.wsu.edu
Reynolds
James
J
Call
Douglas R
DR
Ward
Todd J
TJ
Page
Brent
B
Kadushin
James
J
D015373:Bacterial Typing Techniques; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D016680:Genome, Bacterial; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011137:Polystyrenes
D015342:DNA Probes; D011137:Polystyrenes; D004247:DNA