Comparison of three methods for detecting Campylobacter spp. in chilled or frozen meat.
There is a demand from the meat industry as well as from public health authorities for a simple and rapid detection method for thermophilic Campylobacter spp. from food. Hence, we compared different isolation procedures for their usefulness for this purpose. Bolton enrichment medium without blood, incubated statically in stomacher bags in microaerophilic atmosphere, detected more samples positive for thermophilic Campylobacter spp. than did Preston enrichment broth in bottles with small headspace and tight caps, incubated in aerobic atmosphere. Use of an automated antigen detection system to identify enrichment cultures positive for Campylobacter spp. was as sensitive as selective agars, and reduced the detection time by 24 h. Campylobacter spp. were recovered from 18.4% of the 461 samples tested. The prevalence was highest in refrigerated poultry meat (52% of the 80 samples tested) and poultry offal (41% of the 44 samples tested).
Paulsen
Peter
P
Institute of Meat Hygiene, Meat Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A 1210 Vienna, Austria. peter.paulsen@vu-wien.ac.at
Kanzler
Peter
P
Hilbert
Friederike
F
Mayrhofer
Sigrid
S
Baumgartner
Susanne
S
Smulders
Frans J M
FJ
D000818:Animals; D001431:Bacteriological Techniques; D002167:Campylobacter / Q000302:isolation & purification*; D003080:Cold Temperature; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media