Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases.
Based on previous screening for keratinolytic nonpathogenic fungi, Paecilomyces marquandii and Doratomyces microsporus were selected for production of potent keratinases. The enzymes were purified and their main biochemical characteristics were determined (molecular masses, optimal temperature and pH for keratinolytic activity, N-terminal amino acid sequences). Studies of substrate specificity revealed that skin constituents, such as the stratum corneum, and appendages such as nail but not hair, feather, and wool were efficiently hydrolyzed by the P. marquandii keratinase and about 40% less by the D. microsporus keratinase. Hydrolysis of keratin could be increased by the presence of reducing agents. The catalytic properties of the keratinases were studied and compared to those of some known commercial proteases. The profile of the oxidized insulin B-chain digestion revealed that both keratinases, like proteinase K but not subtilisin, trypsin, or elastase, possess broad cleavage specificity with a preference for aromatic and nonpolar amino acid residues at the P-1 position. Kinetic studies were performed on a synthetic substrate, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide. The keratinase of P. marquandii exhibited the lowest Km among microbial keratinases reported in the literature, and its catalytic efficiency was high in comparison to that of D. microsporus keratinase and proteinase K. All three keratinolytic enzymes, the keratinases of P. marquandii and D. microsporus as well as proteinase K, were significantly more active on keratin than subtilisin, trypsin, elastase, chymotrypsin, or collagenase.
Laboratory of Biotechnology, National Institute of Chemistry, Hajdrihova 19, Ljubljana 1000, Slovenia. helena.gradisar@ki.si
Helena
H
Gradisar
Jozica
J
Friedrich
Igor
I
Krizaj
Roman
R
Jerala
D000595:Amino Acid Sequence; D000818:Animals; D001203:Ascomycota / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010143:Paecilomyces / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D002364:Caseins; D007633:Keratins; D010447:Peptide Hydrolases; C035855:keratinase