[Construction and expression of recombinant human thymosin alpha1 fusion gene in Pichia pastoris].
The human Thymosin alpha1 (hTalpha1) gene was synthesized according to the optimal codons of Pichia pastoris and was fused in 5' terminal of ribosomal protein (RP) gene using over lapping polymerase chain reaction. The fusion gene was inserted into expression vector of pPIC3. 5K and was transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE and Western blot indicated that this fusion protein was expressed. The expression level was about 25mg/L.
Dalian Engineering University, Liao Ning Province, China.
Wen
W
Huang
Zhao-yu
ZY
Li
Li-ji
LJ
Jin
Li-jia
LJ
An