Physicochemical model of alginate-poly-L-lysine microcapsules defined at the micrometric/nanometric scale using ATR-FTIR, XPS, and ToF-SIMS.
Alginate-poly-L-lysine-alginate (APA) microcapsules are currently being investigated as a means to immuno-isolate transplanted cells, but their biocompatibility is limited. In this study, we verified the hypothesis that poly-L-lysine (PLL), which is immunogenic when unbound, is exposed at the APA microcapsule surface. To do so, we analysed the microcapsule membrane at the micrometric/nanometric scale using attenuated total reflectance Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and time-of-flight secondary ion mass spectrometry. The results indicate that PLL and alginate molecules interact within the membrane. PLL exists in considerable amounts near the surface, contributing to the majority of the carbon within the outermost 100 Angstroms of the membrane. PLL was also detected at the true surface (the outermost monolayer) of the microcapsules. The exposure of PLL does not appear to result from defects in the outer alginate coating. This physicochemical model of APA microcapsules could explain their immunogenicity and will play an important role in the optimization of the microcapsule design.
Tam
Susan K
SK
Groupe de Recherche en Biomatériaux/Biomécanique, Ecole Polytechnique de Montréal, C.P. 6079, succ. Centre-ville, Qué., Canada.
Dusseault
Julie
J
Polizu
Stefania
S
Ménard
Martin
M
Hallé
Jean-Pierre
JP
Yahia
L'hocine
L
D000464:Alginates / Q000032:analysis / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D020723:Glucuronic Acid / Q000032:analysis / Q000737:chemistry; D006603:Hexuronic Acids / Q000032:analysis / Q000737:chemistry; D008422:Materials Testing / Q000379:methods; D008832:Microchemistry / Q000379:methods; D008863:Microspheres; D008956:Models, Chemical; D008958:Models, Molecular; D036103:Nanotechnology / Q000379:methods*; D011107:Polylysine / Q000032:analysis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011107:Polylysine; D020723:Glucuronic Acid