Improved routine bio-medical and bio-analytical online fluorescence measurements using fluorescence lifetime resolution.
Fluorescence techniques are widely used as sensitive detection methods in bio-analytics. The use of the bio-physical parameter fluorescence lifetime additional to the spectral characteristics of fluorescence has the potential to improve fluorescence-related detection methods in terms of selectivity in signal recognition, robustness against disturbing influences, and the accessibility of novel bio-chemical process parameters. This article describes the technical set up of a time-resolving instrument with either a fixed time-gated detection principle for improved evaluation of tissue metabolism by an online monitoring of the tissue autofluorescence or a direct fluorescence lifetime detection principle for lifetime-based fluorescent assays.
Pfeifer
Lutz
L
Innovative Optische Messtechnik (IOM) GmbH, Berlin, Germany. info@iom-berlin.de
Stein
Karsten
K
Fink
Ute
U
Welker
Alexander
A
Wetzl
Bianca
B
Bastian
Petra
P
Wolfbeis
Otto S
OS
D000818:Animals; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis; D005415:Flavins / Q000032:analysis; D005453:Fluorescence; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D009206:Myocardium / Q000737:chemistry; D009243:NAD / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013552:Swine
D005415:Flavins; D005456:Fluorescent Dyes; D009243:NAD; D005182:Flavin-Adenine Dinucleotide