Fructosyl-amino acid oxidases of Aspergillus oryzae are induced by the reaction product, glucosone.
Aspergillus oryzae has two fructosyl-amino acid oxidase (FAOD) isozymes (AoFao1 and AoFao2), which are different in the substrate specificities. Northern blot analysis showed both FAO genes were induced by autoclave-browned medium containing l-lysine or l-valine. Studies with a mutant, that had a disrupted AoFAO2 gene, revealed that the expression of AoFAO1 by fructosyl l-valine depended on the expression of AoFAO2. Both genes were also induced by one of the FAOD-reaction products, glucosone. In contrast, other alpha-dicarbonyl compounds, which display a similar structure to that of glucosone were not able to induce the genes expression. These results imply that glucosone may contribute to the expression of FAO genes.
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp
Nobuyuki
N
Yoshida
Shin-ichi
S
Akazawa
Ayako
A
Kuwahara
Tohoru
T
Katsuragi
Yoshiki
Y
Tani
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005800:Genes, Fungal / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007661:Ketoses / Q000378:metabolism*; D008239:Lysine; D013379:Substrate Specificity; D014633:Valine
D003470:Culture Media; D007527:Isoenzymes; D007661:Ketoses; C017022:glucosone; D000594:Amino Acid Oxidoreductases; C103012:amadoriase; D014633:Valine; D008239:Lysine