Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine.
Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.
Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland.
Damien M
DM
O'halloran
Mary T
MT
Cafferkey
D000293:Adolescent; D000328:Adult; D016667:Bacterial Capsules / Q000235:genetics / Q000276:immunology; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007223:Infant; D007494:Ireland; D008875:Middle Aged; D022242:Pneumococcal Vaccines / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018074:Vaccines, Conjugate / Q000276:immunology*
D004269:DNA, Bacterial; D022242:Pneumococcal Vaccines; D018074:Vaccines, Conjugate