Analysis of semicarbazide in baby food by liquid chromatography tandem mass spectrometry (LC-MS-MS)--in-house method validation.
A method for detection of semicarbazide (SEM) in baby food was validated. SEM was extracted with hydrochloric acid and derivatised with 2-nitrobenzaldehyde, using [15N2,13C] semicarbazide as internal standard. The extract was neutralised, purified on a solid phase extraction cartridge and SEM was determined by reversed phase LC-MS-MS. Linearity was demonstrated in the ranges from 0.1 ng ml(-1) to 1 ng ml(-1) and from 2 ng ml(-1) to 80 ng ml(-1). Matrix effects were non significant for meat-based and significant for apple and rice-based baby foods, in both ranges. Mean recoveries ranged from 87.8% to 107.2% with relative standard deviation from 0.2% to 9.1%, considering both ranges. Limits of detection and quantification were 0.1 microg kg(-1) and 0.25 microg kg(-1), respectively. The results of the validation process demonstrated the method suitability for use in food control.
Universidade Federal de Minas Gerais (UFMG), Faculdade de Farmácia (FAFAR), Departamento de Alimentos, Av. AntOnio Carlos 6627, Campus da UFMG, Pampulha, Zip-Code 31.270-010, Belo Horizonte, MG, Brazil.
Scheilla V C
SV
de Souza
Roberto G
RG
Junqueira
Richard
R
Ginn
D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012663:Semicarbazides / Q000032:analysis*; D012680:Sensitivity and Specificity
D012663:Semicarbazides; C010059:carbamylhydrazine