G protein beta 3 subunit gene variants and essential hypertension in the northern Chinese Han population.
Recently a novel C825T polymorphism in the G protein beta3 subunit gene was identified that showed an association with hypertension in a German population; the results of studies in other populations have been inconsistent. To examine the contribution of GNB3 polymorphisms to the development of hypertension in the northern Chinese Han population, we conducted a case-control study consisting of 501 hypertensive cases and 503 controls using the G(-350)A, C825T and C1429T polymorphisms. Genotypes of samples were determined by PCR and restriction digestion. Single locus analysis showed a significant association between G(-350)A and hypertension (P = 0.01) but no association for C825T or C1429T. The three polymorphisms were in tight linkage disequilibrium (D'=-1 for G(-350)A-C825T, D'= 0.92 for C825T-C1429T) and a total of 7 haplotypes were observed in the entire population. Haplotype A-C-C was found to be significantly related to hypertension (P = 0.032) and A-C-C carriers had a more than two-fold higher risk of hypertension than non-carriers, after adjustment for BMI and glucose. In conclusion, our study suggests that G(-350)A is a potential functional polymorphism that may be related to hypertension, whereas the C825T and C1429T polymorphisms are not associated with hypertension in the northern Chinese Han population.
Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Chaoyang District, Beijing 100101, China.
Biao
B
Li
Dongliang
D
Ge
Yuelan
Y
Wang
Weiyan
W
Zhao
Xiaoyang
X
Zhou
Dongfeng
D
Gu
Runsheng
R
Chen
D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D017354:Point Mutation; D011110:Polymorphism, Genetic; D021122:Protein Subunits / Q000235:genetics*
C454420:G-protein beta3 subunit; D021122:Protein Subunits; D020962:Heterotrimeric GTP-Binding Proteins