Superheated water chromatography-nuclear magnetic resonance spectroscopy of kava lactones.
Three kava lactone constituents of Piper methysticum, namely, kawain, methysticin and desmethoxyyangonin, have been separated and identified by reversed-phase HPLC using superheated deuterium oxide as the mobile phase and on-line 1H-NMR detection. The method avoids the use of organic solvents in the mobile phase and hence interferences in the NMR analysis are minimised.
Chienthavorn
Orapin
O
Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UK.
Smith
Roger M
RM
Wilson
Ian D
ID
Wright
Brian
B
Lenz
Eva M
EM
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D017666:Deuterium Oxide / Q000737:chemistry; D020901:Kava / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification*; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification*
D011714:Pyrans; D011753:Pyrones; D017666:Deuterium Oxide; C076881:methysticin; C110481:yangonin; C011889:kavain