Rapid screening of selective serotonin re-uptake inhibitors in urine samples using solid-phase microextraction gas chromatography-mass spectrometry.
In this paper a solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) method is proposed for a rapid analysis of some frequently prescribed selective serotonin re-uptake inhibitors (SSRI)-venlafaxine, fluvoxamine, mirtazapine, fluoxetine, citalopram, and sertraline-in urine samples. The SPME-based method enables simultaneous determination of the target SSRI after simple in-situ derivatization of some of the target compounds. Calibration curves in water and in urine were validated and statistically compared. This revealed the absence of matrix effect and, in consequence, the possibility of quantifying SSRI in urine samples by external water calibration. Intra-day and inter-day precision was satisfactory for all the target compounds (relative standard deviation, RSD, <14%) and the detection limits achieved were <0.4 ng mL(-1) urine. The time required for the SPME step and for GC analysis (30 min each) enables high throughput. The method was applied to real urine samples from different patients being treated with some of these pharmaceuticals. Some SSRI metabolites were also detected and tentatively identified.
Salgado-Petinal
Carmen
C
Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Química, Instituto de Investigación y Análisis Alimentario, Campus Sur, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Lamas
J Pablo
JP
Garcia-Jares
Carmen
C
Llompart
Maria
M
Cela
Rafael
R
D018687:Antidepressive Agents, Second-Generation / Q000652:urine*; D015283:Citalopram / Q000652:urine; D003511:Cyclohexanols / Q000652:urine; D005473:Fluoxetine / Q000652:urine; D016666:Fluvoxamine / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008803:Mianserin / Q000031:analogs & derivatives / Q000652:urine; D000078785:Mirtazapine; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000652:urine*; D020280:Sertraline / Q000652:urine; D052617:Solid Phase Microextraction / Q000379:methods*; D000069470:Venlafaxine Hydrochloride
D018687:Antidepressive Agents, Second-Generation; D015283:Citalopram; D003511:Cyclohexanols; D005473:Fluoxetine; D016666:Fluvoxamine; D008803:Mianserin; D000078785:Mirtazapine; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline; D000069470:Venlafaxine Hydrochloride