Characterization of the 5' regulatory region of the Drosophila Dmdop1 dopamine receptor-gene.
The pharmacological and functional properties of many biogenic-amine receptors have been thoroughly investigated. In contrast, knowledge about the transcriptional regulation of receptor genes is limited. Here we describe the structural and functional properties of the promoter region of a dopamine receptor-gene (Dmdop1) from Drosophila. The transcriptional start site was identified by 5'-RACE (5'-rapid amplification of cDNA ends) cloning and primer-extension analysis. A consensus site for transcriptional initiation (INR element) is located 494 bp upstream of the ATG codon of the open reading-frame. The promoter neither contains TATA- nor CAAT boxes but several GC-rich elements. Relative promoter activity was monitored by CAT reporter-gene analysis in different neuronal cell lines. The Dmdop1 promoter contains one activating (-454/+125) and two silencing regions (-1481/-454 and +125/+495). Interestingly, one silencing region harbours a CRE (cAMP responsive element) site. Since the DmDOP1 receptor leads to cAMP production in cells, the CRE site might contribute to the receptors' own expression by cAMP-dependent transcription factors.
Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Jülich, Postfach 1913, D-52425 Jülich, Germany.
Verena
V
Kehren
Arnd
A
Baumann
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site; D001616:beta-Galactosidase
D017931:DNA Primers; D018076:DNA, Complementary; C093147:Dop1R1 protein, Drosophila; D029721:Drosophila Proteins; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase