Detection of prenatal exposure to several classes of environmental toxicants and their metabolites by gas chromatography-mass spectrometry in maternal and umbilical cord blood.
We developed a sensitive method to detect several classes of pesticides and their metabolites in maternal and cord whole blood using electron-impact gas chromatography-mass spectrometry (GC-MS). The method can detect parent and metabolite compounds at levels of <0.10 and 0.20mug/mL, respectively, with high accuracy and recovery. Analysis of blood from mother-infant dyads from an area of high pesticide use in the Philippines showed detectable levels of propoxur, 3-phenoxybenzoic acid (3-PBA), and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE) in maternal and umbilical cord blood. GC-MS analysis of several classes of parent pesticides and their metabolites in maternal and cord blood provides a sensitive and specific method to detect pesticide exposure during pregnancy.
Corrion
M L
ML
Department of Pediatrics, Hutzel Women's Hospital, Children's Hospital of Michigan, Wayne State University School of Medicine, 4707 St. Antoine, Detroit, MI 48201, USA.
Ostrea
E M
EM
Bielawski
D M
DM
Posecion
N C
NC
Seagraves
J J
JJ
D001565:Benzoates / Q000097:blood; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D004785:Environmental Pollutants / Q000032:analysis* / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D010575:Pesticides / Q000097:blood*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D001074:Propoxur / Q000097:blood
D001565:Benzoates; D004785:Environmental Pollutants; D010575:Pesticides; D003633:Dichlorodiphenyl Dichloroethylene; C017618:3-phenoxybenzoic acid; D001074:Propoxur