Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32.
The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection.
Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
Pricila
P
Hauk
Soledad
S
Negrotto
Eliete Caló
EC
Romero
Sílvio Arruda
SA
Vasconcellos
Margareth Elide
ME
Genovez
Richard John
RJ
Ward
Mirta
M
Schattner
Ricardo Martín
RM
Goméz
Paulo Lee
PL
Ho
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D004357:Drug Synergism; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007921:Leptospira interrogans / Q000145:classification / Q000378:metabolism*; D008074:Lipoproteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins; D011994:Recombinant Proteins; C094114:hemolysin hlx protein, bacteria