[Expression and significance of TERT and c-Myc oncogene protein product on cholesteatoma epithelium].
OBJECTIVE
To detect the expression and localization of human telomerase catalytic subunit (hTERT) and c-Myc oncogene protein product in human middle ear cholesteatoma, and to evaluate their roles in the proliferation of cholesteatoma epithelium.


METHODS
The specimens from the middle ear cholesteatoma tissue of 32 cases and external ear skin of 14 cases were examined by immunohistochemical SP method and computer image analysis system for TERT and c-Myc oncogene protein product.


RESULTS
All 32 samples of cholesteatoma showed a stronger expression of TERT and c-Myc oncogene protein product than that of the external ear skin. The indexes, such as mean positive cell rate and mean obsorbency of cholesteatoma epithelium are much higher than that of the normal external ear shin remarkably( P < 0.05). Accompanied with numerous inflammatory cells(lymphocytes, plasmocytes, neutrophils and macrophages) subcutaneously, the high expression of TERT and c-Myc oncogene protein product were correlated with each other( P < 0. 05).


CONCLUSIONS
Excessive proliferation and keratinization of cholesteatoma are presented by the expression of TERT and c-Myc oncogene protein product in different ways, both of which play a cooperative role in the development of cholesteatoma. Subcutaneous inflammatory response may contribute to the excessive proliferation of keratose cells.
Department of Otorhinolaryngology, Weifang Hospital of Traditional Chinese Medicine, Shandong, 261041, China. zhangfy1@163.com
Fengying
F
Zhang
Jingbo
J
Liu
Tiejun
T
Yuan
Qingming
Q
Liu
Zaiwen
Z
Han
Shuyong
S
Wang
Chuanyi
C
Zhang
Shuping
S
Chen
D000293:Adolescent; D000328:Adult; D000368:Aged; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D019098:Telomerase / Q000096:biosynthesis*
D016271:Proto-Oncogene Proteins c-myc; D019098:Telomerase