Effect of Helicobacter pylori on DNA synthesis of human epithelial cells.
The effect of Helicobacter pylori on DNA synthesis of human cultured cells was examined. Viable H. pylori strains were able to stimulate DNA synthesis by gastric cancer MKN45 cells (eight of eight strains) and laryngeal cancer HEp-2 cells (seven of eight strains) but not by lung cancer A549 cells or fetal intestine Int407 cells. In contrast, neither heat-killed H. pylori nor culture supernatants of H. pylori other than SS-1 strain affected DNA synthesis of MKN45 and HEp-2 cells. In contrast, water extracts of H. pylori strains inhibited DNA synthesis. Soluble outer membrane protein (OMP) of the SS-1 strain stimulated DNA synthesis of MKN45 cells but produced no alteration in cell cycle or apoptosis induction of MKN45 cells. To identify those proteins in the soluble OMP that were capable of stimulating DNA synthesis, the OMP was fractionated by fast performance liquid chromatography with a Mono Q column. The results suggest that two proteins with molecular weights of 18 and 45 kDa, respectively, are associated with stimulation of DNA synthesis by MKN45 cells.
Department of Otolaryngology, Kyorin University School of Medicine, Tokyo, Japan.
Atsushi
A
Toyoda
Takako
T
Osaki
Hiroyuki
H
Yamaguchi
Tomoko
T
Hanawa
Haruhiko
H
Taguchi
Makoto
M
Hasegawa
Shigeru
S
Kamiya
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle; D002460:Cell Line; D002853:Chromatography, Liquid; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008970:Molecular Weight; D012995:Solubility
D001425:Bacterial Outer Membrane Proteins; D004247:DNA