Tumor-infiltrating B cell immunoglobulin variable region gene usage in invasive ductal breast carcinoma.
A major focus of tumor immunology is to reveal the potential role and capacity of immunocompetent cells found in different solid tumor tissues. The most abundant infiltrating cells (TIL), the T lymphocytes have been investigated in details concerning T-cell receptor usage and specificity. However, B cells have hardly been investigated in this respect, although high cellular B-cell infiltration has been correlated with improved patients' survival in some breast carcinomas. This led to our objectives to study variable region gene usage of the tumor-infiltrating B cells in different breast carcinoma types. By defining the immunoglobulin repertoire of the tumor-infiltrating B lymphocytes in the most common invasive ductal carcinoma (IDC) of the breast we compared it to the rare medullary breast carcinoma (MBC). After phenotyping infiltrating ductal carcinomas, B cells were obtained from tumor tissue by microdissection technique. Numerous rearranged TIL-B immunoglobulin heavy chain V genes (VH) were amplified, cloned, sequenced, and comparatively analyzed. Some characteristics were found for both breast carcinoma types. The immunoglobulins produced by TIL-B in ductal carcinoma are highly matured and oligoclonal. We conclude that Ig variable region gene usage reveals similar and distinguishable characteristics of TIL-B immunoglobulin repertoires, which are representative of the nature of the immune responses in invasive ductal and medullary breast carcinomas.
National Medical Center, Institute of Haematology and Immunology, Budapest, H-1135, Hungary.
Peter
P
Simsa
Jean-Luc
JL
Teillaud
David I
DI
Stott
József
J
Tóth
Beatrix
B
Kotlan
D001402:B-Lymphocytes / Q000276:immunology*; D020295:Brain Stem Neoplasms / Q000276:immunology / Q000473:pathology; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000276:immunology* / Q000473:pathology; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D012189:Retrospective Studies
D007135:Immunoglobulin Variable Region