Occurrence of a unique sialyl tetrasaccharide in colostrum of a bottlenose dolphin (Tursiops truncatus).
Crude oligosaccharides were recovered from bottlenose dolphin (Tursiops truncatus) colostrum after chloroform/methanol extraction of lipids and protein precipitation, and purified using gel filtration, anion exchange chromatography and high performance liquid chromatography (HPLC). Their chemical structures characterized by NMR spectroscopy were as follows: GalNAc(beta1-4)[Neu5Ac(alpha2-3)]Gal(beta1-4)Glc, Neu5Ac(alpha2-3)Gal(beta1-4)Glc, Neu5Ac(alpha2-6)Gal(beta1-4)Glc and Gal(alpha1-4)Gal(beta1-4)Glc. The monosialyltetrasaccharide and neutral trisaccharide have not previously been found as free forms in any natural sources including milk or colostrum, although these structures have been found in the carbohydrate units of glycosphingolipids GM2 and Gb3.
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080-8555, Japan.
Yusuke
Y
Uemura
Sadaki
S
Asakuma
Tadashi
T
Nakamura
Ikichi
I
Arai
Michihiro
M
Taki
Tadasu
T
Urashima
D000818:Animals; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003126:Colostrum / Q000737:chemistry*; D004289:Dolphins / Q000378:metabolism*; D005260:Female; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014312:Trisaccharides / Q000032:analysis
D009844:Oligosaccharides; D014312:Trisaccharides; C113460:globotriose; D019158:N-Acetylneuraminic Acid