Multiple roles of vertebrate REV genes in DNA repair and recombination.
In yeast, Rev1, Rev3, and Rev7 are involved in translesion synthesis over various kinds of DNA damage and spontaneous and UV-induced mutagenesis. Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40. REV1-/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants. Surprisingly, the triple-knockout cells showed a suppressed level of sister chromatid exchanges (SCEs), which may reflect postreplication repair events mediated by homologous recombination, while each single mutant showed an elevated SCE level. Furthermore, REV1-/- cells as well as triple mutants showed a decreased level of immunoglobulin gene conversion, suggesting participation of Rev1 in a recombination-based pathway. The present study gives us a new insight into cooperative function of three Rev molecules and the Polzeta (Rev3-Rev7)-independent role of Rev1 in vertebrate cells.
Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
Takashi
T
Okada
Eiichiro
E
Sonoda
Michio
M
Yoshimura
Yoshiaki
Y
Kawano
Hideyuki
H
Saya
Masaoki
M
Kohzaki
Shunichi
S
Takeda
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology; D002460:Cell Line; D002645:Chickens / Q000235:genetics; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000502:physiology*; D005803:Genes, Immunoglobulin / Q000235:genetics; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic / Q000235:genetics*
D004259:DNA-Directed DNA Polymerase