Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology.
In this work, a three-level Box-Behnken factorial design was employed combining with response surface methodology (RSM) to optimize the medium composition for the production of the mycelial biomass and exo-polymer in submerged cultures by Grifola frondosa GF9801. A mathematical model was then developed to show the effect of each medium composition and their interactions on the production of mycelial biomass and exo-polymer. The model estimated that, a maximal yield of mycelial biomass (17.61 g/l) could be obtained when the concentrations of glucose, KH2PO4, peptone were set at 45.2 g/l, 2.97 g/l, 6.58 g/l, respectively; while a maximal exo-polymer yield (1.326 g/l) could be achieved when setting concentrations of glucose, KH2PO4, peptone at 58.6 g/l, 4.06 g/l and 3.79 g/l, respectively. These predicted values were also verified by validation experiments. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a significant increase in the yields of mycelial biomass and exo-polymer. Maximum mycelial biomass yield of 22.50 g/l was achieved in a 15-l fermenter using the optimized medium.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, PR China. fengjiecui@sytu.edu.cn
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D018533:Biomass; D001704:Biopolymers / Q000096:biosynthesis*; D003470:Culture Media / Q000032:analysis*; D005285:Fermentation; D048691:Grifola / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015233:Models, Statistical; D025282:Mycelium / Q000166:cytology / Q000254:growth & development*; D011237:Predictive Value of Tests; D012044:Regression Analysis; D015203:Reproducibility of Results
D001704:Biopolymers; D003470:Culture Media