Variants of the primate vesicular monoamine transporter-2.
The vesicular monoamine transporter-2 (VMAT2) sequesters monoamine neurotransmitters into vesicles and prevents neurotoxicity. Human or monkey striatum generated three VMAT2 immunoreactive proteins of approximately 75 kDa, approximately 52-55 kDa, and approximately 45 kDa. The approximately 55-kDa band is considered the unglycosylated native protein. Deglycosylation of the VMAT2 from striatum or human VMAT2 expressed in HEK293 cells yielded a approximately 45-kDa, but not a 55-kDa immunoreactive band. We investigated this apparent mismatch between observed molecular size and predicted size.
Jassen
Amy K
AK
Department of Psychiatry, Harvard Medical School, New England Primate Research Center, One Pine Hill Drive, Southborough, MA 01772-9102, USA.
Brown
Jeffrey M
JM
Panas
Helen N
HN
Miller
Gregory M
GM
Xiao
Danqing
D
Madras
Bertha K
BK
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000493:pharmacokinetics; D006031:Glycosylation; D006801:Humans; D008253:Macaca mulatta; D046911:Macromolecular Substances / Q000378:metabolism; D008958:Models, Molecular; D008970:Molecular Weight; D016296:Mutagenesis; D010766:Phosphorylation; D014162:Transfection; D014316:Tritium / Q000493:pharmacokinetics; D050493:Vesicular Monoamine Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D046911:Macromolecular Substances; D050493:Vesicular Monoamine Transport Proteins; D014316:Tritium; D004298:Dopamine