Extraction of haptens from solid products and their delivery to the skin, exemplified by dithiocarbamates from rubber gloves.
Allergic contact dermatitis is often caused by solid products such as rubber gloves. Patch testing with the product as is often gives negative results. Extraction of the haptens into an organic solvent is commonly performed to achieve a more correct investigation. The technique used for extraction of haptens from solid materials is only sporadically described. In this study, we investigated and optimized the yields of dithiocarbamates obtained by extraction from rubber gloves. The influence of solvent, extraction time and the procedures for extraction are evaluated. The delivery of zinc dibutyldithiocarbamate from the patch test preparation to the skin is determined.
Department of Dermatology, Lund University, Lund, Sweden.
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O
Bergendorff
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C
Persson
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C
Hansson
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis*; D004050:Ditiocarb / Q000032:analysis*; D017575:Gloves, Protective / Q000009:adverse effects*; D006229:Hand Dermatoses / Q000175:diagnosis; D006241:Haptens / Q000032:analysis*; D006801:Humans; D020315:Latex Hypersensitivity / Q000175:diagnosis*; D010328:Patch Tests / Q000379:methods
D006241:Haptens; D004050:Ditiocarb