[Construction and characterization of Salmonella typhimurium SL7207 SifA- mutant strain].
One characteristic of Salmonella typhimurium SifA- mutant strain is to enter the cytosol of eukaryotic cells. The SifA- mutation of S. typhimurium P3H6 was transferred into S. typhimurium vaccine vector SL7207 by P22 phage transduction and the new strain was named as SL7207*. SL7207* had similar growth curve in LB medium and similar invasiveness for eukaryotic cells. It was found that SL7207* replicates within MDCK cells at a higher rate than SL7207, but is defective for replication in macrophage cell line RAW264.7. SL7207* was more attenuated than SL7207 in BALB/c mice when innoculated intraperitoneally or intravenously. SL7207*, but not SL7207, could transfer eukaryotic expression plasmid into RAW264.7 cells. These results showed that SL7207* could be a promising candidate vaccine vector to express foreign antigen or act as a eukaryotic expression plasmid carrier.
Animal Infectious Diseases Laboratory of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China. zxmyzsj@yahoo.com
Xiao-ming
XM
Zhang
Xin-an
XA
Jiao
Li-hua
LH
Tang
Zhi-ming
ZM
Pan
Yue-lan
YL
Yin
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D017100:Bacteriophage P22; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D014161:Transduction, Genetic; D014613:Vaccines, Attenuated; D014774:Virulence
D001426:Bacterial Proteins; D006023:Glycoproteins; C421862:SifA protein, Salmonella; D014613:Vaccines, Attenuated