Preparative HPLC separation of methoxytetralins, ligands for melatonin receptors, containing two chiral centers with polysaccharide chiral stationary phases. Determination of enantiomeric purity.
Preparative chromatography was used to obtain approximately 200 mg of each of the four individual isomers of the three methoxytetrahydronaphthalenic derivatives, new agonist and antagonist ligands for melatonin receptors to be tested for binding. The mobile and stationary phases were chosen to achieve best resolution in shorter runtime. Enantiomeric purity was verified and quantified using normal and reversed phase methodology on cellulose CSPs. Enantiomer elution order was analysed and discussed. Limit of detection (LOD) and limit of quantification (LOQ) were calculated.
Laboratoire de Chimie Analytique EA 1043, UFR de Physique, Bâtiment P5, Université de Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
Emmanuelle
E
Lipka
Abdelhalim
A
Guelzim
Said
S
Yous
Jean-Paul
JP
Bonte
Claude
C
Vaccher
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008024:Ligands; D044094:Receptors, Melatonin / Q000819:agonists*; D013237:Stereoisomerism; D013764:Tetrahydronaphthalenes / Q000302:isolation & purification*
D008024:Ligands; D044094:Receptors, Melatonin; D013764:Tetrahydronaphthalenes