High-performance liquid chromatographic stationary phases based on poly(dimethylsiloxane) immobilized on silica.
This work describes the preparation and characterization of six stationary phases for high-performance liquid chromatography (HPLC) obtained by deposition of poly(dimethylsiloxane) (PDMS) in HPLC silica particles, followed by immobilization using different processes (thermal treatments, thermal treatment + microwave irradiation, self-immobilization + gamma irradiation and self-immobilization + microwave irradiation). The chromatographic parameters of all the phases were evaluated with a mixture of test compounds having varied natures (acid, basic and neutral). The stability of one of these phases was evaluated in both a neutral mobile phase and a higher pH mobile phase used at an elevated temperature, with promising results.
Tonhi
Edivan
E
Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971, Campinas, SP, Brazil.
Collins
Kenneth E
KE
Collins
Carol H
CH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012822:Silicon Dioxide / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D012822:Silicon Dioxide