Drosophila Mob family proteins interact with the related tricornered (Trc) and warts (Wts) kinases.
The function of Tricornered (Trc), the Drosophila Ndr (Nuclear Dbf2-related) serine/threonine protein kinase, is required for the normal morphogenesis of a variety of polarized outgrowths including epidermal hairs, bristles, arista laterals, and dendrites. In yeast the Trc homolog Cbk1 needs to bind Mob2 to activate the RAM pathway. In this report, we provide genetic and biochemical data that Drosophila Trc also interacts with and is activated by Drosophila Dmob proteins. In addition, Drosophila Mob proteins appear to interact with the related Warts/Lats kinase, which functions as a tumor suppressor in flies and mammals. Interestingly, the overgrowth tumor phenotype that results from mutations in Dmob1 (mats) was only seen in genetic mosaics and not when the entire animal was mutant. We conclude that unlike in yeast, in Drosophila individual Mob proteins interact with multiple kinases and that individual NDR family kinases interact with multiple Mob proteins. We further provide evidence that Mo25, the Drosophila homolog of the RAM pathway hym1 gene does not function along with Trc.
Department of Biology, Center for Morphogenesis and Regenerative Medicine and Cancer Center, University of Virginia, Charlottesville, VA 22903, USA.
Ying
Y
He
Kazuo
K
Emoto
Xiaolan
X
Fang
Nan
N
Ren
Xiaojing
X
Tian
Yuh-Nung
YN
Jan
Paul N
PN
Adler
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011679:Pupa / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D025521:Tumor Suppressor Proteins; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; C110689:MOB1 protein, S cerevisiae; C499633:Mats protein, Drosophila; C100024:Mo25 protein, Drosophila; C506562:Mob2 protein, Drosophila; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; C419769:trc protein, Drosophila; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases