Large-scale screens for cDNAs with in vivo activity.
The pace of biological investigation has been greatly accelerated by technologies that permit comprehensive inventory of gene expression under diverse conditions. We have been developing and using approaches to understand gene function that are based on functional assessments of gene activity, using automated expression cloning followed by gene knockdown using shRNA or by gene knockout. Some recent developments in expression screens and methods to expedite the formation of targeted mutations in mice are discussed. A resource for large scale quantitative profiling of mRNA abundance is also described.
Harvard Medical School, Massachusetts General Hospital, 55 Fruit Street, Molecular Biology, Wellman 911, Boston, MA 02114, USA.
Adrian
A
Ting
Stefan
S
Lichtenthaler
Ramnik
R
Xavier
Soon-Young
SY
Na
Shahrooz
S
Rabizadeh
Tara
T
Holmes
Brian
B
Seed
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling; D051379:Mice; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger