Comparison of phytase production on wheat bran and oilcakes in solid-state fermentation by Mucor racemosus.
Comparisons were made for phytase production using wheat bran (WB) and oilcakes as substrates in solid-state fermentation (SSF) by Mucor racemosus NRRL 1994. WB was also used as mixed substrate with oil cakes. Sesame oil cake (SOC) served as the best carbon source for phytase synthesis by the fungal strain as it gave the highest enzyme titres (30.6 U/gds). Groundnut oil cake (GOC) also produced a reasonably good quantity of enzyme (24.3 U/gds). Enzyme production on WB was surprisingly much less (almost 3.5 times less in comparison to SOC). Mixing WB with SOC (1:1 ratio) resulted in better phytase activity (32.2 U/gds). Optimization of various process parameters such as incubation time, initial moisture content and inoculum concentration was carried out using the single variable mode optimization technique. Under optimized conditions, the production of phytase reached 44.5 U/gds, which was almost 1.5-fold higher than the highest yield obtained with any individual substrate used in this study and was more than 4-fold higher than that obtained from WB.
Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695 019, India.
Krishnan
K
Roopesh
Sumitra
S
Ramachandran
K Madhavan
KM
Nampoothiri
George
G
Szakacs
Ashok
A
Pandey
D010832:6-Phytase / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D004043:Dietary Fiber / Q000378:metabolism*; D005285:Fermentation; D009089:Mucor / Q000737:chemistry / Q000145:classification / Q000201:enzymology*; D010938:Plant Oils / Q000378:metabolism*; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry / Q000378:metabolism
D004043:Dietary Fiber; D010938:Plant Oils; D010832:6-Phytase