A public software for energy filtering transmission electron tomography (EFTET-J): application to the study of granular inclusions in bacteria from Riftia pachyptila.
Energy-filtering transmission electron microscopy (EFTEM) allows the determination of elemental distributions out of a sequence of energy filtered images. Combined with electron tomography, EFTEM is a powerful tool to obtain three-dimensional chemical maps from sub-cellular structures. However, there is no existing software in the public-domain for the computation and analysis of 3D-chemical maps. Here, we present a Java-based program to compute 3D-elemental distribution. This program is available as a set of plug-ins for the public-domain Java image processing program Image J inspired by NIH Image. Its implemented algorithms have been successfully applied to the three-dimensional localization of iron granules in semi thin (200 nm) epon sections from the vent worm Riftia pachyptalia.
Institut Curie, UMR168 and LRC-CEA 34v. 11, rue Pierre et Marie Curie, 75005 Paris, France.
T
T
Boudier
J P
JP
Lechaire
G
G
Frébourg
C
C
Messaoudi
C
C
Mory
C
C
Colliex
F
F
Gaill
S
S
Marco
D000465:Algorithms; D000818:Animals; D001419:Bacteria / Q000166:cytology*; D005629:Frozen Sections; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D002479:Inclusion Bodies / Q000737:chemistry*; D007501:Iron / Q000032:analysis; D046032:Microscopy, Energy-Filtering Transmission Electron; D011077:Polychaeta / Q000382:microbiology*; D012984:Software; D014054:Tomography
D007501:Iron