Spectrofluorometric determination of trace amounts of coenzyme II using norfioxacin-terbium complex as a fluorescent probe.
When terbium ion (Tb3+)-norfloxacin (NFLX) complex is issued a fluorescent probe, in a buffer solution of pH = 7.6, NADP can remarkably enhance the fluorescence intensity of the Tb3+ -NFLX complex at lambda = 545 nm. The enhanced fluorescence intensity of Tb3+ is in proportion to the concentration of NADP. The dynamic range for the determination of NADP is 1.11 x 10(-7) - 6.16 x 10(-5) mol l(-1), with a detection limit of 4.31 x 10(-8) mol l(-1). This method is simple, practical and relatively free of interference from coexisting substances, so it can be successfully applied to determination of NADP in synthetic water samples.
Department of Chemistry, Shandong Normal University, Jinan, 250014, China.
Yusheng
Y
Wang
Jinkai
J
Liu
Chongqiu
C
Jiang
D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009249:NADP / Q000032:analysis* / Q000737:chemistry; D009643:Norfloxacin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*; D014867:Water / Q000032:analysis
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D014867:Water; D013725:Terbium; D009249:NADP; D009643:Norfloxacin