[Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with different metastasis potentials].
OBJECTIVE
To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.


METHODS
Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.


RESULTS
10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.


CONCLUSIONS
The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.
Zhang
Li-jun
LJ
Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Cui
Jie-feng
JF
Liu
Yin-kun
YK
Zhu
Yun-song
YS
Song
Hai-yan
HY
Shen
Hua-li
HL
Feng
Ju-tao
JT
Dai
Zhi
Z
Chen
Jie
J
Sun
Rui-xia
RX
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis; D019000:Phosphotyrosine / Q000032:analysis*
D009363:Neoplasm Proteins; D019000:Phosphotyrosine