The 'anomalous' absorption of labelled and unlabelled vitamin C in man.
Previous studies of vitamin C absorption in man using stable isotope probes have given results which cannot easily be reconciled with those obtained using non-isotope measurement. In order to investigate some of the apparent paradoxes we have conducted a study using two consecutive doses of vitamin C, one labelled and one unlabelled, given 90 min apart. Compatibility of the experimental results with two feasible models was investigated. In Model 1, ingested vitamin C enters a pre-existing pool before absorption, which occurs only when a threshold is exceeded; in Model 2, ingested vitamin C is exchanged with a pre-existing flux before absorption. The key difference between these two models lies in the predicted profile of labelled material in plasma. Model 1 predicts that the second unlabelled dose will produce a secondary release of labelled vitamin C which will not be observed on the basis of Model 2. In all subjects Model 1 failed to predict the observed plasma concentration profiles for labelled and unlabelled vitamin C, but Model 2 fitted the experimental observations. We speculate on possible physiological explanations for this behaviour, but from the limited information available cannot unequivocally confirm the model structure by identifying the source of the supposed flux.
Bluck
Leslie J C
LJ
MRC Human Nutrition Research, Fulbourn Road, Cambridge CB1 9NL, UK. les.bluck@mrc-hmr.cam.ac.uk
Jones
Kerry S
KS
Coward
W Andy
WA
Bates
Christopher J
CJ
D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D001682:Biological Availability; D002247:Carbon Isotopes / Q000493:pharmacokinetics; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D007553:Isotope Labeling; D008297:Male; D008954:Models, Biological
D002247:Carbon Isotopes; D001205:Ascorbic Acid