LanGT2 Catalyzes the First Glycosylation Step during landomycin A biosynthesis.
The glycosyltransferase LanGT2 is involved in the biosynthesis of the hexasaccharide side chain of the angucyclic antibiotic landomycin A. Its function was elucidated by targeted gene inactivation of lanGT2. The main metabolite of the obtained mutant was identified as tetrangulol (4), the progenitor of the landomycin aglycon (7). The lack of the sugar side chain indicates that LanGT2 catalyzes the priming glycosyl transfer in the hexasaccharide biosynthesis: the attachment of a D-olivose to O-8 of the polyketide backbone. Heterologous expression of urdGT2 from S. fradiae Tü2717 in this mutant resulted in the production of a novel C-glycosylated angucycline (6).
Luzhetskyy
Andriy
A
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Taguchi
Takaaki
T
Fedoryshyn
Marta
M
Dürr
Clemens
C
Wohlert
Sven-Eric
SE
Novikov
Volodymyr
V
Bechthold
Andreas
A
D000617:Aminoglycosides / Q000096:biosynthesis*; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D003837:Deoxy Sugars / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation; D013302:Streptomyces / Q000201:enzymology
D000617:Aminoglycosides; D001551:Benz(a)Anthracenes; D003837:Deoxy Sugars; C064648:landomycin A; C407877:olivose; C103670:tetrangulol; D016695:Glycosyltransferases