Proteome response of Escherichia coli fed-batch culture to temperature downshift.
During fed-batch cultivation of Escherichia coli K-12, the proteomic response to a temperature downshift from 37 to 20 degrees C was quantitatively monitored and analyzed by using two-dimensional electrophoresis. When the temperature of exponentially growing E. coli K-12 culture was downshifted to 20 degrees C, the synthesis level of 57 intracellular proteins showed significant changes for a prolonged period of time, compared to the fed-batch culture controlled at 37 degrees C. Thus, these proteins are regarded as important stress proteins responsive to cold shock, which were analyzed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and identified using the E. coli SWISS-2DPAGE database. Most of the identified proteins were shown to be involved in energy metabolism, several cellular molecule biosynthetic pathways and catabolism, cell processes, flagellar biosynthesis and motility, and protein translation and folding. The systematic approach to the monitoring of proteomic responses and the detailed analysis results reported in this article would be useful in understanding the metabolic adaptation to lowered culture temperature and designing efficient fermentation strategies for the production of recombinant proteins and metabolites using E. coli strains.
Kim
Yang-Hoon
YH
Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Ku, Seoul, 136-713, South Korea.
Han
Kyung Yeon
KY
Lee
Kibeom
K
Lee
Jeewon
J
D003080:Cold Temperature; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D048168:Escherichia coli K12 / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
D003470:Culture Media; D029968:Escherichia coli Proteins; D020543:Proteome