Purification of astilbin and isoastilbin in the extract of smilax glabra rhizome by high-speed counter-current chromatography.
Purification of astilbin from the rhizome extract of Smilax glabra was conducted using a high-speed counter-current chromatograph equipped with a 700 mL column. In a single operation, 1.5 g of crude sample was separated to yield 105 mg of component astilbin and 48 mg of isoastilbin while the upper phase of the two-phase solvent system composed of n-hexane-n-butanol-water (1:1:2, v/v/v) was used for stationary phase. The chemical structures of the two flavonoid glycosides were confirmed by electrospray ionization ion trap multiple mass spectrometry and nuclear magnetic resonance experiments.
Du
Qizhen
Q
Institute of Food and Biological Engineering, Zhemjiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang 310035, China. qizhendu@mail.zjgsu.edu.cn
Li
Lei
L
Jerz
Gerold
G
D003377:Countercurrent Distribution / Q000379:methods*; D044948:Flavonols / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*; D032281:Smilax / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D044948:Flavonols; D010936:Plant Extracts; C099069:astilbin