Complementary function of gamma delta T-lymphocytes and dendritic cells in the response to isopentenyl-pyrophosphate and lipopolysaccharide antigens.
Dendritic cells and gamma delta T-lymphocytes play a crucial role in the early response to microbial infections. Since both dendritic cells and gamma delta T-lymphocytes may be activated by specific microbial products, we analyzed their interplay in the presence of such respective ligands: lipopolysaccharide and isopentenyl-pyrophosphate. Activated gamma delta T-cells increased the maturational state of dendritic cells induced by lipopolysaccharide, increasing the expression of co-stimulatory and MHC class I and II molecules. IL-12 production by dendritic cells was strongly amplified in the presence of activated gamma delta T-cells and the Th1 polarization of naïve CD4(+) T-lymphocytes was significantly increased. On the other hand, dendritic cells enhanced gamma delta T-cell functions induced by isopentenyl-pyrophosphate and promote their IL-2 independent proliferation through CD86 contact. Altogether, dendritic cells and gamma delta T-cells exert a complementary function promoting an optimal immune response to non peptidic microbial antigens.
Martino
Angelo
A
Unit of Cellular Immunology, National Institute for Infectious Diseases Lazzaro Spallanzani - IRCCS, Rome, Italy. martino@inmi.it
Casetti
Rita
R
D'Alessandri
Alessandra
A
Sacchi
Alessandra
A
Poccia
Fabrizio
F
D015703:Antigens, CD / Q000502:physiology; D051940:B7-2 Antigen; D002450:Cell Communication / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D045782:Hemiterpenes / Q000494:pharmacology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000502:physiology; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells
D015703:Antigens, CD; D051940:B7-2 Antigen; C497964:CD86 protein, human; D045782:Hemiterpenes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D009943:Organophosphorus Compounds; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018664:Interleukin-12; C004809:isopentenyl pyrophosphate