Intriguing mass spectrometric behavior of guanosine under low energy collision-induced dissociation: H2O adduct formation and gas-phase reactions in the collision cell.
An in-depth study of the fragmentation pathway of guanosine was conducted by using an in-source collision-induced dissociation high-mass accuracy tandem mass spectrometry experiment. The equivalent of MS4 data, a level of information normally achieved on ion trap instruments, was obtained on a Q-TOF mass spectrometer. The combination of the features of high-resolution, accuracy, and in-source CID permitted the unambiguous elucidation of the different fragmentation pathways. Furthermore the elemental compositions of the product ions generated were assigned and their mutual genealogical relationships established. Formerly proposed dissociation pathways of guanosine were revisited and elaborated on more deeply. Furthermore, the presence of H2O in the collision cell of several tandem MS instruments was demonstrated and its effect on the product ion spectra investigated. The neutral gain of H2O by particular fragments of guanosine was experimentally proven by using argon, saturated with H2(18)O, as the collision gas. Data indicating the occurrence of more complex reactions in the collision cell as a result of the presence of H2O were produced, specifically relating to neutral gain/neutral loss sequences. In silico calculations supported the experimental observation of neutral gain by guanosine fragments and predicted a similar behavior for adenosine. The latter was subsequently experimentally confirmed.
Tuytten
Robin
R
Department of Chemistry, Nucleoside Research Unit [corrected] University of Antwerp, Antwerp, Belgium.
Lemière
Filip
F
Van Dongen
Walter
W
Esmans
Eddy L
EL
Witters
Erwin
E
Herrebout
Wouter
W
Van Der Veken
Benjamin
B
Dudley
Ed
E
Newton
Russell P
RP
D001128:Argon; D004735:Energy Transfer; D006151:Guanosine / Q000032:analysis* / Q000737:chemistry*; D013058:Mass Spectrometry; D010103:Oxygen Isotopes; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry
D010103:Oxygen Isotopes; D010446:Peptide Fragments; D014867:Water; D006151:Guanosine; D001128:Argon