Detection of human metapneumovirus from children with acute otitis media.
Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.
Virus Research Center, Sendai Medical Center, Sendai, Japan.
Akira
A
Suzuki
Oshi
O
Watanabe
Michiko
M
Okamoto
Hiroko
H
Endo
Hisakazu
H
Yano
Mitsuko
M
Suetake
Hidekazu
H
Nishimura
D000208:Acute Disease; D000818:Animals; D001424:Bacterial Infections / Q000150:complications / Q000382:microbiology; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004432:Ear, Middle; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification*; D010033:Otitis Media / Q000382:microbiology / Q000821:virology*; D018184:Paramyxoviridae Infections / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000150:complications / Q000821:virology; D014777:Virus Diseases / Q000150:complications / Q000821:virology