Capillary electrophoretic method for the determination of diterpenoid isomers in Acanthopanax species.
Acanthoic, continentalic and kaurenoic acids are bioactive diterpenoids that are structural isomers isolated from Acanthopanax species. Due to the interest in their potent biological activity, an analytical method of diterpenoids was developed for the quality control and the classification of Acanthopanax species. Capillary electrophoresis was used to separate and quantify the isomers. The three compounds were successfully separated from each other and from the matrices in the extracts of leaves, stems and roots of Acanthopanax species. The contents of acanthoic, continentalic and kaurenoic acids showed taxonomical differences in Acanthopanax species. Relatively higher concentrations of diterpenoids were found from A. koreanum and A. trifoliatus, while only trace amounts were found from the four other species tested: A. senticosus, A. senticosus f. inermis, A. chiisanensis, and A. divaricatus var. albeofructus. The contents of diterpenoids in association with lignans and triterpenoids in the Acanthopanax species could provide a chemotaxonomical index able to be used in the classification and discrimination of the species.
College of Pharmacy, Chungnam National University, Yu-Seong-Gu, Daejeon 305764, Republic of Korea.
Nguyen Thi
NT
Phuong
Kyung Ah
KA
Lee
Su Jin
SJ
Jeong
Cai Xing
CX
Fu
Jung Kap
JK
Choi
Young Ho
YH
Kim
Jong Seong
JS
Kang
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D004586:Electrophoresis; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D028001:Eleutherococcus / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008956:Models, Chemical; D010936:Plant Extracts; D015203:Reproducibility of Results; D013997:Time Factors
D002021:Buffers; D004224:Diterpenes; D010936:Plant Extracts; C099958:acanthoic acid; C510689:continentalic acid; C051436:kaurenoic acid