[Study on apoptosis of human cervical carcinoma HeLa cells with RNA interference targeting hTERT].
OBJECTIVE
To study the induction of tumor cell apoptosis by RNA interference-mediated inhibition of the expression of telomerase in cancer cells.


METHODS
HeLa cells were transfected with the successfully established siRNA(small interfering RNA) expression vectors targeting hTERT (human telomerase reverse transcriptase). By electronic microscopy, Western blot and FCM (flow cytometry), the apoptosis of HeLa cells was tested.


RESULTS
The established siRNA expression vectors could induce apoptosis of HeLa cells.


CONCLUSIONS
Transfection of siRNA expression vectors targeting hTERT can induce apoptosis of HeLa cells.
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. zhaoying@fmmu.edu.cn
Ying
Y
Zhao
Jun-lin
JL
Ren
Rui
R
Zhang
Long-yang
LY
Ma
Wei
W
Bao
Cheng-ji
CJ
Wang
An-gang
AG
Yang
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D034622:RNA Interference; D019098:Telomerase / Q000235:genetics*; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
D019098:Telomerase