[Effects of hypothermia on antioxidant capacity of rat testes after testicular torsion].
OBJECTIVE
To investigate the effects of hypothermia on the antioxidant capacity of rat testes after testicular torsion.


METHODS
Twenty-four healthy pubertal male Sprague-Dawley rats were randomly divided into three groups of equal number: Group A (torsion) , Group B (torsion + hypothermia) and Group C (control). The animals were submitted to unilateral 720 degrees testicular torsion, and underwent detorsion two hours later. Fourteen days later, the total antioxidant capacity (T-AOC) and the level of malonic diethylaldehyde(MDA) were detected with spectrophotometer and histological changes were observed by light microscope.


RESULTS
The T-AOC of Group B was significantly greater than that of Group A (P < 0.01), but less than that of Group C (P < 0.01). The MDA level of Group B was lower than that of Group A (P < 0.01), but higher than that of Group C (P < 0.05).


CONCLUSIONS
Hypothermia can restrain the production of oxygen free radicals following testicular torsion/detorsion in rats, which in turn can inhibit lipid peroxidation and increase the survivability of the torsional testis.
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Hai-Tao
HT
Chen
Xin-Min
XM
Zheng
Zhi-Wei
ZW
Yang
Hang
H
Zheng
Shi-Wen
SW
Li
Li-Quan
LQ
Hu
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D007036:Hypothermia, Induced; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000473:pathology / Q000503:physiopathology*; D013737:Testis / Q000473:pathology
D000975:Antioxidants; D008315:Malondialdehyde