Coxsackievirus B3 replication is related to activation of the late extracellular signal-regulated kinase (ERK) signal.
MAP kinase signaling has been implicated in coxsackievirus B3 (CVB3) pathogenesis and as necessary in the virus lifecycle. We studied the correlation with extracellular signal-regulated kinase 1/2 (ERK1/2) signaling and virus replication in the presence of coxsackievirus and adenovirus receptor (CAR). In CHO cells that do not expressed CAR, specific ERK1/2 phosphorylation (pERK1/2) was not detected, and progeny virus was not produced after infection. By contrast, in HeLa and CHO-CAR cells, which expressed CAR, the specific early and late pERK1/2 at 0.5 and 8 h were induced, and progeny viruses were produced progressively through 24 h after infection. However, when CHO-CAR cells were infected with replication-defective CVB3, specific pERK1/2 was not detected. In addition, when late pERK1/2 is inhibited by the MEK1 inhibitor PD98059, at 4 h after infection, virus replication significantly decreased. Therefore, our findings suggest that early pERK1/2 is a response to virus binding to CAR, whereas late pERK1/2 is related to the viral replication.
Department of Medicine, Sungkyunkwan University School of Medicine, Cardiac and Vascular Center, Samsung Medical Center, 50 Il-Won Dong, Seoul 135-710, South Korea.
Byung-Kwan
BK
Lim
Jae-Hwan
JH
Nam
Chae-Ok
CO
Gil
Soo-Hyeon
SH
Yun
Jin-Ho
JH
Choi
Duk-Kyung
DK
Kim
Eun-Seok
ES
Jeon
D000818:Animals; D016466:CHO Cells; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006224:Cricetinae; D029822:Enterovirus B, Human / Q000235:genetics / Q000502:physiology*; D005419:Flavonoids / Q000494:pharmacology; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D015398:Signal Transduction; D014779:Virus Replication
D005419:Flavonoids; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048052:Mitogen-Activated Protein Kinase 3; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one