Immunoblotting analysis of somatic components of Dirofilaria immitis.
SDS-PAGE analysis of Dirofilaria immitis extracts demonstrated the complexity of somatic protein component of adult male similar to that of adult female worm. Western blot analysis showed six major peptide bands of 85, 66, 42, 20, 16.2 and 14.5 kDa recognized in the sera of infected dogs. Western blotting sera from dogs with Dirofilaria immitis infection suggest that antigenic components in the low molecular weight region may be related to the anti-parasitic mechanism of the host.
Oge
H
H
Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, Ankara. oge@veterinary.ankara.edu.tr
Oge
S
S
Yildirim
A
A
Kircali
F
F
Kara
M
M
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D006790:Host-Parasite Interactions; D008297:Male; D008970:Molecular Weight
D000947:Antigens, Helminth