Molecular properties of membrane-bound FAD-containing D-sorbitol dehydrogenase from thermotolerant Gluconobacter frateurii isolated from Thailand.
There are two types of membrane-bound D-sorbitol dehydrogenase (SLDH) reported: PQQ-SLDH, having pyrroloquinoline quinone (PQQ), and FAD-SLDH, containing FAD and heme c as the prosthetic groups. FAD-SLDH was purified and characterized from the PQQ-SLDH mutant strain of a thermotolerant Gluconobacter frateurii, having molecular mass of 61.5 kDa, 52 kDa, and 22 kDa. The enzyme properties were quite similar to those of the enzyme from mesophilic G. oxydans IFO 3254. This enzyme was shown to be inducible by D-sorbitol, but not PQQ-SLDH. The oxidation product of FAD-SLDH from D-sorbitol was identified as L-sorbose. The cloned gene of FAD-SLDH had three open reading frames (sldSLC) corresponding to the small, the large, and cytochrome c subunits of FAD-SLDH respectively. The deduced amino acid sequences showed high identity to those from G. oxydans IFO 3254: SldL showed to other FAD-enzymes, and SldC having three heme c binding motives to cytochrome c subunits of other membrane-bound dehydrogenases.
Toyama
Hirohide
H
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. hirot@yamaguchi-u.ac.jp
Soemphol
Wichai
W
Moonmangmee
Duangtip
D
Adachi
Osao
O
Matsushita
Kazunobu
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D017124:Conserved Sequence; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D020576:Gluconobacter / Q000201:enzymology*; D007064:L-Iditol 2-Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013012:Sorbitol / Q000378:metabolism; D013785:Thailand
D001426:Bacterial Proteins; D008565:Membrane Proteins; D005182:Flavin-Adenine Dinucleotide; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase