Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn, New York: epidemiology and recommendations for detection.
Among 257 isolates of Klebsiella pneumoniae collected in Brooklyn, NY, 24% were found to possess bla(KPC). Clinical microbiology laboratories that used automated broth microdilution systems reported 15% of the KPC-possessing isolates as susceptible to imipenem. The imipenem MIC was found to be markedly affected by the inoculum. For accurate detection of KPC-possessing K. pneumoniae, particular attention should be paid to proper inoculum preparation for broth-based susceptibility methods. In addition, using ertapenem or meropenem for class reporting of carbapenem susceptibility will improve detection.
Bratu
Simona
S
Department of Medicine, SUNY-Downstate Medical Center, Brooklyn, New Yoek 11203, USA.
Mooty
Mohamad
M
Nichani
Satyen
S
Landman
David
D
Gullans
Carl
C
Pettinato
Barbara
B
Karumudi
Usha
U
Tolaney
Pooja
P
Quale
John
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D006287:Health Planning Guidelines; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D007753:Laboratories / Q000592:standards; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D008829:Microbiology; D009519:New York City / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D001618:beta-Lactamases