Use of water to evaluate hydrophobicity of organically-modified xerogel enzyme supports.
Silica xerogels are a new class of materials suitable for the immobilization of enzymes for various applications including biotransformations and biosensors. The physicochemical properties of xerogels, such as hydrophobicity, can be manipulated by the introduction of organically-modified silicates. This allows the immobilization matrix to be engineered to suit the enzyme and its application. Interfacial activation of lipase is a phenomenon in which the enzyme displays increased activity when it is bound to a hydrophobic interface. Lipase was entrapped in organically-modified xerogels in which the hydrophobicity of the enzyme support was modulated by the selection of different alkyltrimethoxysilane co-precursors and the ratio in which they were combined with tetramethyl orthosilicate. Interaction between the enzyme support and water was investigated with two methods to quantitatively assess the hydrophobicity of the entrapment matrix. The contact angle formed between the xerogel and water was used to determine hydrophobicity on a macroscopic level. Temperature-controlled water desorption was used to determine hydrophobicity on a microscopic level. Both methods were suitable for quantitatively discriminating between hydrophobic and hydrophilic materials. Further, the hydrophobicity of the enzyme support influenced the hydrolytic activity of the entrapped lipase under non-aqueous conditions. The specific activity of lipase increased only when entrapped in xerogels which could be classified as hydrophobic materials, that is with contact angles greater than 90 degrees or hydrophobicity values as determined by water desorption greater than 0.65.
Department of Chemical Engineering, University of Waterloo, Ontario, Canada.
Joseph S
JS
Clifford
Raymond L
RL
Legge
D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000032:analysis* / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000032:analysis / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D014867:Water; D058428:Silica Gel; D012822:Silicon Dioxide; D008049:Lipase