[Influence of macrophages on the expression of vascular endothelial growth factor receptor mRNA, homeobox B2 mRNA, and integrin alpha nu beta3 in vascular endothelial strain].
OBJECTIVE
To investigate the influence of macrophages on the expression of the vascular endothelial growth factor (VEGF) receptor (KDR) mRNA, homeobox B2 (HOXB2) mRNA, and integrin alpha nu beta3 in vitro in vascular endothelial strain.


METHODS
Human umbilical vein cells (ECV304) were cultured in vitro and divided into 4 groups, i.e. (1) ECV304 group, (2) ECV304 + conA group [with conA (25 microg/ml in culture) added to ECV304], (3) ECV304 + U937 group (with 1 x 10(5)/ml of U937 cells added to 1 x 10(5)/ml ECV 304), (4) ECV304 + U937 + conA group [with 1 x 10(5)/ml of U937 cells and conA (25 microg/ml in culture)] groups. Forty-eight hours after culturing, the expression of integrin receptor alpha nu beta3 and the changes in the expression of KDR mRNA and HOXB2 mRNA in each group were determined by immunofluorescent technique and RT-PCR, respectively.


RESULTS
The expression of integrin receptor alpha nu beta3, KDR mRNA, and HOXB2 mRNA in ECV304 group were 6.7 +/- 1.5, 0.633 +/- 0.012, and 0.674 +/- 0.004, respectively, while those in ECV304 + U937 + conA group (10.2 +/- 1.7, 0.879 +/- 0.003, 0.947 +/- 0.003) were obviously more upregulated when compared with those in ECV304 group (P < 0.01). No difference in the above indices was found between ECV304 and ECV304 + conA, ECV304 + U937 groups (P > 0.05).


CONCLUSIONS
Macrophages activated by ConA can accelerate the proliferation, migration and adhesion to the basement membrane matrix of vascular endothelial cells through the influence on the expression of KDR mRNA, HOXB2 mRNA and integrin alpha nu beta3, and through this pathway the angiogenesis is modulated.
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Liang
L
Liu
Chang
C
Liu
Xiao-qi
XQ
Zhang
Jia
J
Ming
Xu-sheng
XS
Liu
Hui
H
Xu
Tian-min
TM
Cheng
D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
C086061:HOXB2 protein, human; D018398:Homeodomain Proteins; D039302:Integrin alphaVbeta3; D012333:RNA, Messenger; D014157:Transcription Factors; D040262:Receptors, Vascular Endothelial Growth Factor