Engineering of a vascularized scaffold for artificial tissue and organ generation.
Tissue engineering is an emerging field in regenerative medicine to overcome the problem of end-stage organ failure. However, complex tissues and organs need a vascular supply to guaranty graft survival and render bioartificial organ function. Here we developed methods to decellularize porcine small bowl segments and repopulate the remaining venous and arterial tubular structures within these matrices with allogeneic porcine endothelial progenitor cells. Cellular adherence and vitality was characterized by quantitative 2-[18F]-fluoro-2'-desoxy-glucose (FDG) positron emission tomography (PET) and subsequent immunohistological work up. The generated matrices showed insulin-dependent FDG uptake predominantly in the region of the former vascular structures. Stain for vitality and the specific endothelial markers CD31, VE-Cadherin and Flk-1 matched this functional finding. Providing evidence for vitality up to 3 weeks post reconstitution and typical endothelial differentiation, these results indicate that our generated matrix allows the generation of complex bioartificial tissues and organs for experimental and future clinical application.
Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, Germany. heike.mertsching@igb.fraunhofer.de
Heike
H
Mertsching
Thorsten
T
Walles
Michael
M
Hofmann
Johanna
J
Schanz
Wolfram H
WH
Knapp
D000818:Animals; D015703:Antigens, CD; D021522:Bioartificial Organs; D001854:Bone Marrow Cells / Q000166:cytology; D015820:Cadherins / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D004247:DNA / Q000737:chemistry; D004727:Endothelium / Q000166:cytology; D004730:Endothelium, Vascular / Q000378:metabolism; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry; D007150:Immunohistochemistry; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D049268:Positron-Emission Tomography; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D004247:DNA; D019788:Fluorodeoxyglucose F18; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D000099152:Cadherin 5