Use of biodegradable PLGA microspheres as a slow release delivery system for the Boophilus microplus synthetic vaccine SBm7462.
The synthetic anti-Boophilus microplus vaccine SBm7462 derived from the tick intestinal protein, Bm86, induced a protective immune response when emulsified in saponin and used in cattle. Using a mice model, and with the objective of improving the vaccine by continual peptide release, it was encapsulated in PLGA 50:50 microspheres and inoculated in BALB/c mice to assess the immunological response by detection of anti-peptide IgGs. Comparative studies were made with the peptide emulsified in saponin and with another synthetic vaccine, and the microsphere/peptide was characterized for efficiency of encapsulation, in vitro release profile, morphology, size, peptide integrity after encapsulation and stability in different pHs. The findings showed that saponin enhances a better immune response from SBm7462 and that the PLGA 50:50 microspheres are suitable for use with this peptide.
Laboratory of Biology and Control of Haematozoa and Vectors, BIOAGRO/Veterinary Department, Federal University of Viçosa, 36571-000 Viçosa, MG, Brazil.
P A
PA
Sales-Junior
F
F
Guzman
M I
MI
Vargas
S
S
Sossai
A M
AM
Patarroyo V
C Z L
CZ
González
J H
JH
Patarroyo
D000818:Animals; D001673:Biodegradation, Environmental; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D003692:Delayed-Action Preparations; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D026863:Ixodidae / Q000276:immunology*; D019344:Lactic Acid; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D013983:Tick Control; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000378:metabolism / Q000633:toxicity
D003692:Delayed-Action Preparations; D007074:Immunoglobulin G; D011108:Polymers; D014614:Vaccines, Synthetic; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid