Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin.
To improve the production of the lantibiotic subtilin in Bacillus subtilis ATCC 6633, two genetic engineering strategies were followed. Firstly, additional copies of subtilin self-protection (immunity) genes spaIFEG have been integrated into the genome of the producer strain. Their expression significantly enhanced the subtilin tolerance level, and concomitantly, the subtilin yield 1.7-fold. Secondly, a repressor of subtilin gene expression, the B. subtilis general transition state regulator protein AbrB, was deleted. A sixfold enhancement of the subtilin yield could be achieved with the abrB deletion mutant; however, the produced subtilin fraction predominantly consists of succinylated subtilin species with less antimicrobial activity compared to unmodified subtilin.
Heinzmann
Stefan
S
Institut für Mikrobiologie, Johann Wolfgang Goethe-Universität, Marie-Curie-Str. 9, 60439, Frankfurt am Main, Germany.
Entian
Karl-Dieter
KD
Stein
Torsten
T
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics; D004361:Drug Tolerance; D017353:Gene Deletion; D018628:Gene Dosage; D005818:Genetic Engineering; D016680:Genome, Bacterial; D008074:Lipoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D019802:Succinic Acid / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; C060762:AbrB protein, Bacillus subtilis; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D004268:DNA-Binding Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010455:Peptides; C104626:SpaB protein, Bacillus subtilis; C089314:SpaF protein, Bacillus subtilis; C089316:SpaG protein, Bacillus subtilis; C089313:SpaI protein, Bacillus subtilis; D014157:Transcription Factors; D019802:Succinic Acid; C002836:subtilin