Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA. cartermar@mail.nih.gov
Mark G
MG
Carter
Alexei A
AA
Sharov
Vincent
V
VanBuren
Dawood B
DB
Dudekula
Condie E
CE
Carmack
Charlie
C
Nelson
Minoru S H
MS
Ko
D000818:Animals; D001483:Base Sequence; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D016678:Genome; D007438:Introns; D051379:Mice; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger