Oral administration of a live Aro attenuated Salmonella vaccine strain expressing 14-kDa Schistosoma mansoni fatty acid-binding protein induced partial protection against experimental schistosomiasis.
We report the oral vaccination of SWISS mice with an Aro attenuated Salmonella enterica var. Typhimurium vaccine strain expressing the 14-kDa Schistosoma mansoni antigen, Sm14. Bacterial adjuvants, including (i) Lactococcus lactis expressing interleukin-12 (IL-12) and (ii) Lactobacillus delbrueckii UFV-H2b20, were also employed in oral immunization assays. Detection assays to specific IgG and IgA anti-Sm14 antibodies were performed to evaluate humoral immune responses in vaccinated mice. An increase in specific IgG titers was observed; however, no IgA production was detected. The protection levels against schistosomiasis (34.9-49.5%) obtained with all experimental formulations in this work were very similar to values reported by previous studies, which used purified recombinant Sm14 for parenteral vaccination of mice. There was a slight reduction in hepatic granulomas of mice vaccinated with Salmonella. Oogram studies showed diminished numbers of S. mansoni eggs in the intestinal wall of vaccinated mice, but individual female worm fecundity did not seem to be affected by our immunization protocol.
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627-Pampulha, Belo Horizonte, MG 31270-901, Brazil.
Luis G C
LG
Pacheco
Eder
E
Zucconi
Vitor L T
VL
Mati
Rachel M
RM
Garcia
Anderson
A
Miyoshi
Sergio C
SC
Oliveira
Alan L
AL
de Melo
Vasco
V
Azevedo
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D050612:Fatty Acid Transport Proteins; D005260:Female; D015801:Helminth Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology*; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D022562:Salmonella Vaccines / Q000627:therapeutic use*; D012550:Schistosoma mansoni / Q000187:drug effects*; D012552:Schistosomiasis / Q000276:immunology* / Q000517:prevention & control
D000947:Antigens, Helminth; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; D022562:Salmonella Vaccines; C068709:SM14 protein, Schistosoma mansoni