The two Cg-timp mRNAs expressed in oyster hemocytes are generated by two gene families and differentially expressed during ontogenesis.
We previously characterized a Crassostrea gigas tissue inhibitor of metalloproteinase (Cg-timp 1.3) with potential role in wound healing and defense mechanisms. Here we isolated a second cDNA (Cg-timp 1.1) encoding a protein that contains the characteristic signature of TIMP proteins. Sequence analysis of the two transcripts showed that they originate from two distinct genes. The two proteins, Cg-TIMP 1.1 and 1.3, are closely related and share 81% identity. Northern blot analysis of Cg-timp gene expression in adult oyster hemocytes indicated that the ratio between the two transcripts was constant from one oyster to another (Cg-timp 1.1 and 1.3 represent 32 and 68%, respectively). Conversely, during ontogenesis the expression pattern of the two Cg-timp genes was different. Indeed, Cg-timp 1.3 mRNAs were detected from the larval D stage whereas Cg-timp 1.1 transcripts were undetectable up to 22 days post-fertilization. The difference in expression pattern of the two Cg-timp genes may reveal distinct implications of these genes in the embryos and larvae developments.
Génome, Populations, Interactions, Adaptation (GPIA), UMR5171 (IFREMER, CNRS, UMII) Université de Montpellier II, place Eugène Bataillon, CC80, 34095 Montpellier, France.
Caroline
C
Montagnani
Ana
A
Tirape
Viviane
V
Boulo
Jean-Michel
JM
Escoubas
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010125:Ostreidae / Q000196:embryology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; C431265:TIMP protein, Crassostrea gigas; D019714:Tissue Inhibitor of Metalloproteinases