Enzymatic polymerization behavior using cellulose-binding domain deficient endoglucanase II.
A mutant enzyme, EGII(core), in which the cellulose-binding domain was deleted from endoglucanase II from Trichoderma viride, was expressed in yeast, and the secreted enzyme was examined for the enzymatic polymerization to obtain artificial cellulose. EGII(core) polymerized beta-cellobiosyl fluoride to afford crystalline cellulose of type II. Comparison of the polymerization behavior of EGII(core) with that of EGII revealed the following: i) the crystalline product obtained with EGII(core) was stable in the polymerization solution, although the product was readily hydrolyzed in the presence of EGII; ii) the turnover number of EGII(core) was as high as that of EGII; iii) EGII(core) produced highly crystalline cellulose. EGII(core) is therefore advantageous for enzymatic polymerization.
Nakamura
Itsuko
I
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Yoneda
Hisanari
H
Maeda
Toshifumi
T
Makino
Akira
A
Ohmae
Masashi
M
Sugiyama
Junji
J
Ueda
Mitsuyoshi
M
Kobayashi
Shiro
S
Kimura
Shunsaku
S
D002475:Cellobiose / Q000031:analogs & derivatives* / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000096:biosynthesis* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis; D011134:Polysaccharides / Q000138:chemical synthesis; D017434:Protein Structure, Tertiary / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017384:Sequence Deletion; D014242:Trichoderma / Q000201:enzymology*
D011108:Polymers; D011134:Polysaccharides; C074099:cellobiosyl fluoride; D002475:Cellobiose; D002482:Cellulose; C056373:endoglucanase 2; D002480:Cellulase