Synthesis of DNA conjugate by mechanochemical solid-state polymerization and its affinity separation of oligonucleotides having single-base difference by capillary electrophoresis.
In this communication, we discuss the characterization of DNA conjugate synthesized by mechanochemical polymerization, Con-M, on the separation of model oligo-DNA and its single nucleotide polymorphisms (SNPs) by affinity capillary electrophoresis, compared with that prepared by radical-initiated solution polymerization, Con-RL. The average molecular weight of Con-M was similar to that of Con-RL, although the molecular weight distribution of Con-M was narrower than that of Con-RL. Capillary electrophoresis of oligo-DNA was performed using the capillary filled with DNA conjugate. The resolution of the capillary filled with Con-M was apparently higher than with Con-RL. It is considered that higher resolution using the capillary filled with Con-M could be ascribed not only to the narrow molecular weight distribution but also to the difference of copolymer structure.
Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University, Japan. skondo@gifu-pu.ac.jp
Shin-ichi
S
Kondo
Daisuke
D
Shichijyou
Yasushi
Y
Sasai
Yukinori
Y
Yamauchi
Masayuki
M
Kuzuya
D001704:Biopolymers; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D020641:Polymorphism, Single Nucleotide
D001704:Biopolymers; D009841:Oligonucleotides; D004247:DNA