Chemoenzymatic synthesis of 6omega -modified maltooligosaccharides from cyclodextrin derivatives.
Regioselectively substituted maltooligosaccharides were prepared by enzymatic transformation of modified cyclodextrins by using simultaneously two different enzymes: cyclodextrin glucanotransferase (CGTase) and amyloglucosidase. Oligosaccharides were obtained in very good yields and their structures were identified by 1D and 2D NMR spectroscopy. These results provided new information about the specificity of the catalytic sites of CGTase and amyloglucosidase. They also offered new ways for the synthesis of regioselectively modified maltooligosaccharides.
Fraschini
Carole
C
Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), FR CNRS 2607, BP 53, 38041 Grenoble cedex 9, France.
Greffe
Lionel
L
Driguez
Hugues
H
Vignon
Michel R
MR
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000737:chemistry*; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D014312:Trisaccharides / Q000737:chemistry
D002241:Carbohydrates; D003505:Cyclodextrins; D009844:Oligosaccharides; D014312:Trisaccharides; C021705:maltooligosaccharides; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose