Amyloid-beta oligomers are inefficiently measured by enzyme-linked immunosorbent assay.
Amyloid-beta (Abeta) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Abeta results in underestimated Abeta ELISA levels. The implications are that comprehensive analysis of soluble Abeta requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.
Stenh
Charlotte
C
Department of Public Health/Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
Englund
Hillevi
H
Lord
Anna
A
Johansson
Ann-Sofi
AS
Almeida
Claudia G
CG
Gellerfors
Pär
P
Greengard
Paul
P
Gouras
Gunnar K
GK
Lannfelt
Lars
L
Nilsson
Lars N G
LN
D000544:Alzheimer Disease / Q000175:diagnosis; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D014162:Transfection
D016229:Amyloid beta-Peptides