Structural and biological characterization of aminated-derivatized oat beta-glucan.
beta-Glucan extracted from oats was subjected to reductive amination, producing a cationic beta-glucan derivative of which physiological properties were characterized. The degree of substitution was obtained from elemental analysis, which was 0.48. In addition, the distribution of amino groups in the beta-glucan derivative was investigated by FT-IR analysis. In vitro bile acid binding capacity of the aminated beta-glucan was examined, showing significantly higher bile acid binding activity than native beta-glucan. Moreover, the beta-glucan derivative showed pronounced antimicrobial effects against Escherichia coli and Bacillus subtilis, and ACE (angiotensin-converting enzyme) inhibition activities which were dependent on its concentration. Furthermore, bronchoalveolar lavage (BAL) experiments demonstrated that the beta-glucan derivative stimulated the synthesis of nitric oxide. The improved functionalities of the derivative could be explained by its polycationic characteristics.
Shin
Min Su
MS
Department of Food and Nutrition, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Korea.
Lee
Suyong
S
Lee
Kwang Yeon
KY
Lee
Hyeon Gyu
HG
D000588:Amines / Q000737:chemistry*; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents; D018554:Avena / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D001647:Bile Acids and Salts / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002412:Cations; D004926:Escherichia coli / Q000187:drug effects; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D000588:Amines; D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D002412:Cations; D005936:Glucans; D009569:Nitric Oxide