Arsenite oxidation in batch reactors with alginate-immobilized ULPAs1 strain.
Arsenic is one of the major groundwater contaminants worldwide. It was previously demonstrated that the beta-proteobacterium Cenibacterium arsenoxidans has an efficient As[III] oxidation ability. The present study was conducted to evaluate the performance of alginate-immobilized ULPAs1 in the oxidation of As[III] to As[V] in batch reactors. A two-level full factorial experimental design was applied to investigate the influence of main parameters involved in the oxidation process, i.e., pH (7-8), temperature (4 degrees C-25 degrees C), kind of nutrient media (2%-20% sauerkraut brine), and arsenic concentration (10-100 mg/L). One hundred milligram per liter of As[III] was fully oxidized by calcium-alginate immobilized cells in 1 h. It was found that the temperature as well as the kind of nutrient media used were significant parameters at a 95% confidence interval whereas only temperature was a significant parameter at a 99% confidence interval. The immobilization of the As[III] oxidizing strain in alginate beads offers a promising way to implement new treatment processes in the remediation of arsenic contaminated waters.
Laboratoire de Dynamique, Evolution et Expression de Génomes de Microorganismes, FRE 2326 Université Louis Pasteur-CNRS, 28 rue Goethe, 67083 Strasbourg Cédex, France.
Diliana D
DD
Simeonova
Kalina
K
Micheva
Daniel A E
DA
Muller
Florence
F
Lagarde
Marie-Claire
MC
Lett
Veneta I
VI
Groudeva
Didier
D
Lièvremont
D000464:Alginates / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism*; D020562:Betaproteobacteria / Q000378:metabolism*; D019149:Bioreactors; D018914:Cells, Immobilized / Q000378:metabolism*; D003470:Culture Media; D005618:Fresh Water; D010084:Oxidation-Reduction; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism
D000464:Alginates; D003470:Culture Media; D014874:Water Pollutants, Chemical; D001151:Arsenic