Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in zebrafish.
The doublesex and mab-3-related transcription factor 1 (Dmrt1) is the founding member of a family of DM domain genes, involved in gonadogenesis. Here we report the cloning of zebrafish Sox5 and use real time PCR to show that its expression increases while expression of Dmrt1 decreases during embryogenesis. Characterization of the proximal promoter of zebrafish Dmrt1 revealed two positive and two negative regulatory regions and a Sox5-binding site. Co-transfection of dmrt1 (with or without the Sox-binding site), driving an EGFP reporter and Sox5 showed further that Sox5 bound the Dmrt1 promoter and inhibited Dmrt1 expression. This antagonistic partnership between Dmrt1 and Sox5 suggests a potential transcriptional regulatory mechanism for Dmrt1 in early embryogenesis.
Gao
Shang
S
Department of Genetics and Center for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Zhang
Tao
T
Zhou
Xiang
X
Zhao
Yang
Y
Li
Qin
Q
Guo
Yiqing
Y
Cheng
Hanhua
H
Zhou
Rongjia
R
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015536:Down-Regulation; D004625:Embryo, Nonmammalian; D005260:Female; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055755:SOXD Transcription Factors; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics*
C110547:DMRT1 protein; D012097:Repressor Proteins; D055755:SOXD Transcription Factors; C506396:Sox5 protein, zebrafish; D014157:Transcription Factors; D029961:Zebrafish Proteins