[Relationship Between Molecular Marker of Western Main Pig H-FABP Gene and IMF Content.].
By using 265 pigs from eight breeds including Duroc,Landrace,Large White,Neijiang,Rongchang,Hanjiang Black,Hanzhong White,Bamei and wild ones, the genetic variations of 5'-upstream region from and the second intron in porcine H-FABP gene were checked by PCR-RFLP molecular marker with HinfI, Hae III and MspI,and effect of H-FABP gene on IMF content was then analyzed by least square analysis.The results showed as follows:(1) 8 pig breeds and wild pig had polymorphism at Hinf I-RFLP site. In above detected breeds,large white,Bamei pig, Hanjiang Black,Hanzhong White pig breeds and wild pig presented low polymorphism while other breeds have mediate polymorphism;(2)Among the tested breeds only 4 Chinese local pig breeds had no polymorphism at the Hae III-RFLP and Msp I-RFLP sites,but Duroc,Landrace,Largewhite, Hanzhong White pig breeds and wild pig had polymorphism. Wild pig at the Hae III-RFLP , Landrace,Largewhite and wild pig at the Hae III-RFLP and Msp I-RFLP sites were low polymorphism,others were mediate polymorphism;(3) H-FABP gene increased IMF content significantly(p0.05). Genetic effect of H-FABP gene on IMF content were HH>Hh>hh,DD.
College of Animal Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China, Email: pwj1226@hotmail.com.
Wei-Jun
WJ
Pang
Shi-Duo
SD
Sun
Ying
Y
Li
Guo-Dong
GD
Chen
Gong-She
GS
Yang
D000818:Animals; D001947:Breeding; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa; D013552:Swine