L-NAME administration prevents the inhibition of nucleotide hydrolysis by rat blood serum subjected to hyperargininemia.
The main objective of the present study was to evaluate the in vivo and in vitro effect of Arg on serum nucleotide hydrolysis. The action of Nomega-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase, on the effects produced by Arg was also examined. Sixty-day-old rats were treated with a single or a triple (with an interval of 1 h between each injection) intraperitoneal injection of saline (group I), Arg (0.8 g/kg) (group II), L-NAME (2.0 mg/kg or 20 mg/kg) (group III) or Arg (0.8 g/kg) plus L-NAME (2.0 mg/kg or 20 mg/kg) (group IV) and were killed 1 h later. The present results show that a triple Arg administration decreased ATP, ADP and AMP hydrolysis. Simultaneous injection of L-NAME (20 mg/kg) prevented such effects. Arg in vitro did not alter nucleotide hydrolysis. It is suggested that in vivo Arg administration reduces nucleotide hydrolysis in rat serum, probably through nitric oxide or/and peroxynitrite formation.
Delwing
D
D
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Gonçalves
M C F
MC
Sarkis
J J F
JJ
Wyse
A T S
AT
D000244:Adenosine Diphosphate / Q000097:blood / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000097:blood / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000097:blood / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D006868:Hydrolysis / Q000187:drug effects; D020162:Hyperargininemia / Q000097:blood*; D066298:In Vitro Techniques; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage*; D009711:Nucleotides / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009711:Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester