[The Paris experience in preimplantation genetic diagnosis: evaluation after the first births].
OBJECTIVE
To report the birth of the first thirteen infants conceived after preimplantation genetic diagnosis (PGD) within the medical assistance federation of Paris.


METHODS
Fifty-nine couples were enrolled between January 2000 and July 2001. They had a total of 71 oocyte pick-up cycles. The collected oocytes were inseminated by intracytoplasmic sperm injection. The resulting embryos were biopsied on the third day of development and the genetic analysis was performed on the same day. Most of the embryo transfers were carried out on the fourth day.


RESULTS
The 71 oocyte pick-up cycles yielded 872 oocytes of which 731 were suitable for intracytoplasmic sperm injection. 421 embryos were biopsied and genetic diagnosis was obtained from 312 (74%) of these. 127 embryos were transferred during the course of 58 transfer procedures. There were 18 biologic and 12 clinical (7 singles, 4 twins and 1 triple) pregnancies. Thirteen infants have been born and 4 are expected.


CONCLUSIONS
PGD has gained a place among the choices offered to couples at risk of transmission of a serious and incurable genetic disease.
Frydman
N
N
Service d'Histologie-Embryologie-Cytogénétique à orientation Biologique et Génétique de la Reproduction, Hôpital Antoine Béclère (AP-HP), 157, rue de la Porte de Trivaux, 92141 Clamart.
Romana
S
S
Ray
P
P
Hamamah
S
S
Tachdjian
G
G
Marcadet-Fredet
S
S
Munnich
A
A
Vekemans
M
M
Frydman
R
R
D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D010297:Paris; D011247:Pregnancy; D011296:Prenatal Diagnosis; D020554:Sperm Injections, Intracytoplasmic