Bone marrow stromal cells generate muscle cells and repair muscle degeneration.
Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow.
Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoecho, Sakyo-ku, Kyoto, 606-8501 Japan. dezawa@anat2.med.kyoto-u.ac.jp
Mari
M
Dezawa
Hiroto
H
Ishikawa
Yutaka
Y
Itokazu
Tomoyuki
T
Yoshihara
Mikio
M
Hoshino
Shin-ichi
S
Takeda
Chizuka
C
Ide
Yo-ichi
Y
Nabeshima
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002459:Cell Fusion; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000032:analysis; D006801:Humans; D051379:Mice; D018101:Mice, Inbred mdx; D008819:Mice, Nude; D032342:Muscle Cells / Q000166:cytology*; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D009124:Muscle Proteins / Q000032:analysis; D018482:Muscle, Skeletal / Q000166:cytology; D009135:Muscular Diseases / Q000628:therapy*; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D020880:Neuregulins / Q000494:pharmacology; D051763:PAX7 Transcription Factor; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D014162:Transfection
D018398:Homeodomain Proteins; D009124:Muscle Proteins; D020880:Neuregulins; D051763:PAX7 Transcription Factor; C497321:PAX7 protein, human; C497322:Pax7 protein, mouse; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D005576:Colforsin