Bone marrow cell-seeded biodegradable polymeric scaffold enhances angiogenesis and improves function of the infarcted heart.
BACKGROUND
The present study examined whether a bioengineered polyglycolic acid cloth (PGAC) impregnated with bone marrow cells (BMC) improved the function and angiogenesis of the infarcted heart.


RESULTS
The coronary artery was ligated in Lewis rats and the infarcted area was covered with a PGAC in group 1 (n=8), with a PGAC containing basic-fibroblast growth factor (b-FGF) in group 2 (n=11) and a PGAC containing b-FGF and freshly isolated BMC in group 3 (n=10). In addition, BMC derived from transgenic mice expressing green fluorescent protein (GFP)-BMC were seeded into a PGAC, which was sutured over the infarcted area of C57BL/6 mice (n=5). In the rat study, developed and systolic pressures, dp/dt max and dp/dt min) were the highest in group 3, as were the capillary density in the PGAC and infarcted area. In the mouse study, there were few GFP-BMC in the PGAC, but none in the infarcted area.


CONCLUSIONS
A PGAC with BMC improved cardiac function by inducing angiogenesis without migration of BMC. Freshly isolated BMC work as angiogenic inducers and a PGAC is useful as a "drug delivery system".
Department of Regenerative Medicin, National Cardiovascular Center, Suita, Japan.
Shinya
S
Fukuhara
Shinji
S
Tomita
Takeshi
T
Nakatani
Toshiya
T
Fujisato
Yoshinori
Y
Ohtsu
Michiko
M
Ishida
Chikao
C
Yutani
Soichiro
S
Kitamura
D020341:Absorbable Implants; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D051379:Mice; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D018919:Neovascularization, Physiologic; D011100:Polyglycolic Acid; D051381:Rats; D011917:Rats, Inbred Lew
D011100:Polyglycolic Acid