Protection of turkeys against Chlamydophila psittaci challenge by DNA and rMOMP vaccination and evaluation of the immunomodulating effect of 1 alpha,25-dihydroxyvitamin D(3).
Plasmid DNA expressing the major outer membrane protein (MOMP) of an avian Chlamydophila psittaci serovar D strain and recombinant MOMP (rMOMP) with or without the immunomodulating adjuvant 1 alpha,25-dihydroxyvitamin D(3) have been tested for their ability to elicit an immune response and induce protection in turkeys against challenge with the same serovar. Three vaccination strategies were compared: priming and boosting with either pcDNA1::MOMP or rMOMP and priming with pcDNA1::MOMP followed by rMOMP boosting. Turkeys primed with pcDNA1::MOMP showed significant protection against Cp. psittaci challenge, turkeys primed with rMOMP did not. The steroid hormone 1 alpha,25-dihydroxyvitamin D(3) augmented serum and mucosal antibody titres. However, higher antibody titres were not related to better protection and even had a negative effect on especially bacterial excretion.
Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure Links 653, Ghent B-9000, Belgium. kristel.verminnen@UGent.be
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Verminnen
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Loock
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Cox
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Goddeeris
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Vanrompay
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002117:Calcitriol / Q000494:pharmacology*; D002691:Chlamydophila psittaci / Q000276:immunology*; D008213:Lymphocyte Activation; D011201:Poultry Diseases / Q000517:prevention & control*; D009956:Psittacosis / Q000517:prevention & control / Q000662:veterinary*; D014422:Turkeys; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C078167:OMPA outer membrane proteins; D002117:Calcitriol