Somatostatin receptor subtype 2-mediated scintigraphy and localization using (99m)Tc-HYNIC-Tyr(3)-octreotide in human hepatocellular carcinoma-bearing nude mice.
OBJECTIVE
To investigate the uptake of (99m)Tc-HYNIC-Tyr(3)-octreotide ((99m)Tc-HYNIC-TOC) in human hepatocellular carcinoma (HCC), which can provide the localizable diagnosis in hepatic carcinoma.


METHODS
The expression of somatostatin receptor 2 (SSTR2) messenger RNA (mRNA) in human HCC cell line HepG(2) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). Uptake of (99m)Tc-HYNIC-TOC was evaluated in the human HCC implanted into BALB/c nude mice. ANMIS2000 nuclear medicine analysis system was used to calculate the ratio of (99m)Tc uptake between tumor tissue and vital organs.


RESULTS
We demonstrated the expression of SSTR2 mRNA in human HCC cell line HepG(2) by RT-PCR. The size of the RT-PCR products was 364 bp detected by sequence analysis of the human SSTR2 mRNA. Scintigraphy proved that (99m)Tc-HYNIC-TOC was uptaken in the tumor tissue, liver and kidney of the tumor-bearing mice.


CONCLUSIONS
Based on expression of the SSTR2 mRNA in human HCC, (99m)Tc-HYNIC-TOC can markedly bind with and be uptaken by human HCC tissues as compared with normal liver tissue. The significant retention of radionuclide in kidney and bladder is probably related to non-specific peptide uptake in the tubulus cells of kidney and possibly due to excretion by kidney. Our results show that localizable diagnosis and targeting radiotherapy with radionuclide-labeled somatostatin analog for HCC are of great value to be further studied.
Li
Yong
Y
Gastrointestinal Laboratory of Clinical Medical Institute of Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.
Si
Jian-Ming
JM
Zhang
Jun
J
Du
Jin
J
Wang
Fan
F
Jia
Bing
B
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging* / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015282:Octreotide / Q000031:analogs & derivatives; D015609:Organotechnetium Compounds; D012333:RNA, Messenger / Q000378:metabolism; D011877:Radionuclide Imaging; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D015609:Organotechnetium Compounds; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; C410263:technetium Tc 99m hydrazinonicotinyl-Tyr(3)-octreotide; C093733:somatostatin receptor 2; D015282:Octreotide