Human gastric tissues simultaneously express the classical and alternative splicing cholecystokinin-B/gastrin receptors.
To explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant preserving intron 4 (CCKBRi4sv) are expressed in human gastric carcinoma cell line and tissue, we detect mRNA expression of CCKBRwt and CCKBRi4sv in 30 gastric carcinoma and their corresponding normal tissues, 10 gastritis, and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells by RT-PCR and sequencing. The results revealed that human normal, inflammatory, and malignant gastric tissues simultaneously expressed the classical and alternative splicing cholecystokinin-B/gastrin receptor genes. The alternative splicing variant contains the intron 4 of cholecystokinin-B/gastrin receptor gene.
Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Sichuan University, Chengdu, China. zjjdjb@hotmail.com
Jianjiang
J
Zhou
Manling
M
Chen
Qunzhou
Q
Zhang
Jiankun
J
Hu
Wenling
W
Wang
D017398:Alternative Splicing; D001483:Base Sequence; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000378:metabolism; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000378:metabolism
D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B