Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus.
Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 microl greatly reduced the number of isolates requiring induction.
Miller
Melissa B
MB
University of North Carolina Hospitals, University of North Carolina School of Medicine, Campus Box 7525, Chapel Hill, NC 27599-7525, USA. mbmiller@unch.unc.edu
Meyer
Heather
H
Rogers
Elaine
E
Gilligan
Peter H
PH
D001426:Bacterial Proteins / Q000235:genetics*; D003030:Coagulase / Q000378:metabolism; D006801:Humans; D007841:Latex Fixation Tests; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins / Q000378:metabolism*; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C487789:mecA protein, Staphylococcus aureus; D010068:Oxacillin