Splenic marginal zone lymphoma: 7q abnormalities.
7q abnormalities are the most common cytogenetic or genetic aberrations found in splenic marginal zone lymphoma. The molecular methods whereby these regions of genetic loss can be characterized are discussed in this chapter. Emphasis is given to careful experimental design, for example sample purification and optimization, that is, ensuring that only target sequences are amplified. There also is discussion of result interpretation, pitfalls that may be encountered, and alternative visualization techniques that might be used.
Molecular Biology Department, Royal Bournemouth Hospital, UK.
Rachel E
RE
Ibbotson
Anton E
AE
Parker
David G
DG
Oscier
D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D019656:Loss of Heterozygosity; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D013160:Splenic Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm