Supercritical fluid extraction of sinomenine from Sinomenium acutum (Thumb) Rehd et Wils.
Supercritical carbon dioxide, with and without a methanol modifier, was used to extract sinomenine from Sinomenium acutum (Thumb) Rehd et Wils. Sinomenine determinations were carried out using high-performance liquid chromatography (HPLC). The results show that the yield obtained after 2.5 h extraction with methanol-modified supercritical carbon dioxide was the highest (7.47 mg/g), while that obtained with only supercritical carbon dioxide was the lowest (0.17 mg/g). The recovery obtained with supercritical carbon dioxide, with and without a methanol modifier, could not be increased greatly by the method of the alkalinization of sample. Higher recoveries were obtained than extraction using methanol in Soxhlet extractor.
Liu
Ben
B
Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. liu_ben0@hotmail.com
Jiang
Hailiang
H
Shen
Bo
B
Chang
Yiling
Y
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D009019:Morphinans / Q000302:isolation & purification*; D032841:Sinomenium / Q000737:chemistry*
D009019:Morphinans; C009271:sinomenine