Egg-white protein fractionation using new weak anion-exchange resins based on poly(glycidyl methacrylate-co-ethylendimethacrylate). Preparation and characterization.
A new formulation for a poly(glycidyl methacrylate-co-ethylendimethacrylate)-based resin with a 11:9 proportion of monomer to crosslinker is developed and amino-functionalized in order to obtain new particulate materials suitable for egg-white protein fractionation. Functionalization is carried out using three different chemical reagents: diethylamine (DEA), DEA-tetrahydrofuran (THF) (1:1), and concentrated ammonia. The ammonia- and DEA-THF-treated polymers are used to fractionate egg-white proteins, in particular lysozyme and ovalbumin, by anion-exchange chromatography in packed column experiments, the latter resin showing better performances. Finally, both supports, working at semipreparative scale and step-gradient elution, separate pure ovalbumin with a yield of 83%.
Paredes
B
B
Department of Chemical Engineering and Environmental Technology, Oviedo University, Oviedo, Spain.
González
S
S
Rendueles
M
M
Villa-García
M A
MA
Díaz
J M
JM
D000837:Anion Exchange Resins; D002853:Chromatography, Liquid / Q000379:methods*; D004527:Egg Proteins / Q000302:isolation & purification*; D004529:Egg White / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008768:Methylmethacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010316:Particle Size
D000837:Anion Exchange Resins; D004527:Egg Proteins; D008768:Methylmethacrylates; C020347:GMA-EDMA