[Congener specific determination of PCBs in human blood using gel permeation chromatography (GPC) and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS)].
We studied the progressive analytical method for polychlorinated biphenyls (PCBs) in blood samples collected from Yusho patients. This study established a systematic procedure for determining both "total concentration of PCBs and their profiles" and "concentrations of mono-ortho PCBs as dioxin-like compounds", providing a significant index for diagnosis. The method we developed consists of a rapid clean-up using gel permeation chromatography (GPC) and sensitive identification with high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). This method provides identification of all of PCB congeners typically detected in human blood samples, especially it is worth noting that nine significant congeners on Yusho diagnosis (IUPAC #105, #114, #118, #123, #153, #156, #157, #167, #189) were detected from a five-gram blood sample. Using the present method, we analyzed sixty-six individual blood samples collected during the annual Yusho inspection in the 2004, and could identified sixty-eight PCB isomers in all samples. As a result, ten patients were classified into pattern A, the typical Yusho pattern. The mean total PCB concentration of pattern A subjects was 2.95 ppb, while that of control subjects was 0.77 ppb, at whole blood basis respectively.
Hori
Tsuguhide
T
Fukuoka Institute of Health and Environmental Sciences, Fukuoka 818-0135, Japan.
Tobiishi
Kazuhiro
K
Ashizuka
Yuki
Y
Nakagawa
Reiko
R
Todaka
Takashi
T
Hirakawa
Hironori
H
Iida
Takao
T
D015415:Biomarkers / Q000097:blood; D002850:Chromatography, Gel / Q000379:methods*; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012275:Oryza / Q000506:poisoning; D010938:Plant Oils / Q000506:poisoning; D011041:Poisoning / Q000175:diagnosis; D011078:Polychlorinated Biphenyls / Q000097:blood* / Q000506:poisoning
D015415:Biomarkers; D010938:Plant Oils; D011078:Polychlorinated Biphenyls