Factors affecting the free radical scavenging behavior of chitosan sulfate.
Scavenging activity of hydroxyethyl chitosan sulfate (HCS) against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl and carbon-centered radical species were studied using electron spin resonance (ESR) spectroscopy. In addition, its antioxidant activity to retard lipid peroxidation was also evaluated in a linoleic acid model system. HCS could scavenge DPPH (33.78%, 2.5 mg/mL) and carbon-centered radicals (67.74%, 0.25 mg/mL) effectively. However, chitosan sulfate did not exhibit any scavenging activity against hydroxyl radicals, but increased its generation. This was different from the published literature and was presumed due to the loss of chelating ability on Fe2+. This assumption could further confirm from the results obtained for Fe2+-ferrozine method that upon sulfation chitooligosaccharides lost its chelation properties. Therefore, HCS can be identified as antioxidant that effectively scavenges carbon centered radicals to retard lipid peroxidation.
Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Ronghua
R
Huang
Eresha
E
Mendis
Se-Kwon
SK
Kim
D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds; D002244:Carbon / Q000737:chemistry; D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D007202:Indicators and Reagents / Q000494:pharmacology; D007501:Iron / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009844:Oligosaccharides / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D007202:Indicators and Reagents; D009844:Oligosaccharides; D010851:Picrates; C480355:chitosan sulfate; D002686:Chitin; D002244:Carbon; D048271:Chitosan; D004492:Edetic Acid; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron