Up-regulation expression of MLC1 in human liver cancer tissue and enhanced SMMC7721 cell tumorigenesis in vivo and vitro.
OBJECTIVE
We screened a novel gene MLC1 in human liver cancer tissue by differential display, and its cDNA full-length is 1600bp. The purpose of this study is to find expression of MLC1 gene in human liver cancer tissue and the affect to SMMC7721 cell tumorigenesis in vivo and vitro.


METHODS
250 cases of primary HCC tissue samples were studied for MLC1 mRNA and protein expression using RT-PCR, western blot, immunohistochemistry, MLC1 stable transfection into SMMC771, and SMMC7721 cells growth curve was analyzed by MTT method and SMMC7721 cells tumorigenesis in vivo.


RESULTS
RT-PCR results showed that 98.1% (245/250) was MLC1 up-regulation expression, 1.9% (5/250) was MLC1 down-regulation (p<0.01). Immunohistochemistry showed that 97.2% (243/250) was MLC1 up-regulation expression, 2.8% (7/250) was down-regulation (p<0.01) in cancer tissue compared with paracancerous tissue (p<0.01). Western blotting results showed that 98.9% (247/250) was MLC1 up-regulation expression, 1.1% (3/250) was MLC1 down-regulation. Overexpression of MLC1 enhanced the growth of SMMC7721 cells compared with the control cells. pcDNA3.1-MLC1 cells accelerated tumor formation compared with pcDNA3.1.


CONCLUSIONS
MLC1 gene showed up-regulation expression at both the mRNA and protein levels in HCC tissues and that MLC1 plays an important role in the growth of hepatoma cell SMMC7721 in vitro and vivo.
Dong-Dong
Lu
L
Tsinghua Institute of Genome Research, Institute of Biomedicine, School of Medicine, Tsinghua University Beijing, PR China. Ludondon88@163.com
D000328:Adult; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000378:metabolism*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology* / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
C425069:MLC1 protein, human; D008565:Membrane Proteins; C498927:Mlc1 protein, mouse; D012333:RNA, Messenger