Use of a locked-nucleic-acid oligomer in the clamped-probe assay for detection of a minority Pfcrt K76T mutant population of Plasmodium falciparum.
Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting analysis with a mutation-specific detection probe.
Department of Parasitology, Rangueil University Hospital, 31059 Toulouse 9, France.
Alice
A
Senescau
Antoine
A
Berry
Françoise
F
Benoit-Vical
Olfert
O
Landt
Richard
R
Fabre
Joël
J
Lelièvre
Sophie
S
Cassaing
Jean-François
JF
Magnaval
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D002738:Chloroquine / Q000494:pharmacology; D003062:Codon; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics*; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D026901:Membrane Transport Proteins; D009154:Mutation; D021261:Parasitic Sensitivity Tests / Q000379:methods; D020135:Peptide Nucleic Acids; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000962:Antimalarials; D003062:Codon; D016054:DNA, Protozoan; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D020135:Peptide Nucleic Acids; C416369:PfCRT protein, Plasmodium falciparum; D015800:Protozoan Proteins; D002738:Chloroquine