Trichinella nativa in a black bear from Plymouth, New Hampshire.
A suspected case of trichinellosis was identified in a single patient by the New Hampshire Public Health Laboratories in Concord, NH. The patient was thought to have become infected by consumption of muscle larvae (ML) in undercooked meat from a black bear killed in Plymouth, NH in October 2003 and stored frozen at -20 degrees C fro 4 months. In January 2004, a 600 g sample of the meat was thawed at 4 degrees C, digested in hydrochloric acid and pepsin, and larvae were collected by sedimentation. Intact, coiled, and motile ML were recovered (366 larvae per gram (l pg) of tissue), which were passed into mice and pigs. Multiplex PCR revealed a single 127 bp amplicon, indicative of Trichinella nativa. The Reproductive Capacity Index (RCI) for the T. nativa-Plymouth isolate in mice was 24.3. Worm burdens in the diaphragms of two 3-month-old pigs given 2,500 ML were 0.05 and 0.2l pg by 35 days post-inoculation, while 2.2 and 0.75 l pg were recovered from two 3-month-old pigs given 10,000 ML; no larvae were recovered from four 1-year-old pigs given 2,500 ML (n=2) or 10,000 ML (n=2). Viable larvae were also recovered from frozen black bear meat harvested at two additional locations, one in southern Ontario, Canada, and one in upstate New York, USA. Multiplex PCR using genomic DNA from these parasite samples demonstrated that both isolates were T. nativa. This is the first report of the freeze-resistant species, T. nativa, within the continental United States.
Hill
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United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1044, BARC-East, Beltsville, MD 20705, USA. dhill@anri.barc.usda.gov
Gamble
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Zarlenga
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DS
Coss
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Finnigan
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D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005260:Female; D016266:Food Parasitology; D007814:Larva; D008460:Meat / Q000469:parasitology; D051379:Mice; D009132:Muscles / Q000469:parasitology; D009513:New Hampshire; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014234:Trichinella / Q000235:genetics / Q000302:isolation & purification*; D014235:Trichinellosis / Q000469:parasitology*; D001503:Ursidae / Q000469:parasitology*; D015047:Zoonoses / Q000469:parasitology*
D018509:DNA, Helminth