Interaction of the N-terminal segment of pulmonary surfactant protein SP-C with interfacial phospholipid films.
Pulmonary surfactant protein SP-C is a 35-residue polypeptide composed of a hydrophobic transmembrane alpha-helix and a polycationic, palmitoylated-cysteine containing N-terminal segment. This segment is likely the only structural motif the protein projects out of the bilayer in which SP-C is inserted and is therefore a candidate motif to participate in interactions with other bilayers or monolayers. In the present work, we have detected intrinsic ability of a peptide based on the sequence of the N-terminal segment of SP-C to interact and insert spontaneously into preformed zwitterionic or anionic phospholipid monolayers. The peptide expands the pi-A compression isotherms of interfacial phospholipid/peptide films, and perturbs the lipid packing of phospholipid films during compression-driven liquid-expanded to liquid-condensed lateral transitions, as observed by epifluorescence microscopy. These results demonstrate that the sequence of the SP-C N-terminal region has intrinsic ability to interact with, insert into, and perturb the structure of zwitterionic and anionic phospholipid films, even in the absence of the palmitic chains attached to this segment in the native protein. This effect has been related with the ability of SP-C to facilitate reinsertion of surface active lipid molecules into the lung interface during respiratory compression-expansion cycling.
Plasencia
Ines
I
Departamento de Bioquímica, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain.
Keough
Kevin M W
KM
Perez-Gil
Jesus
J
D000327:Adsorption; D020816:Amino Acid Motifs; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002412:Cations; D003545:Cysteine / Q000737:chemistry; D007477:Ions; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010169:Palmitic Acids / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011312:Pressure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D037721:Pulmonary Surfactant-Associated Protein C / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013552:Swine; D013997:Time Factors
D002412:Cations; D007477:Ions; D008055:Lipids; D010169:Palmitic Acids; D010455:Peptides; D010743:Phospholipids; D037721:Pulmonary Surfactant-Associated Protein C; D003545:Cysteine