Comparative study on radiosensitivity of various tumor cells and human normal liver cells.
OBJECTIVE
To investigate the radiation response of various human tumor cells and normal liver cells.


METHODS
Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with (60)Co gamma-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A.


RESULTS
Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired.


CONCLUSIONS
The results suggest that the gamma-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.
Yang
Jian-She
JS
Radiobiology Laboratory, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China. tuyangjs@impcas.ac.cnit
Li
Wen-Jian
WJ
Zhou
Guang-Ming
GM
Jin
Xiao-Dong
XD
Xia
Jing-Guang
JG
Wang
Ju-Fang
JF
Wang
Zhuan-Zi
ZZ
Guo
Chuan-Ling
CL
Gao
Qing-Xiang
QX
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000528:radiation effects; D008113:Liver Neoplasms / Q000473:pathology*