Complex formation between cationically modified gold nanoparticles and DNA: an atomic force microscopic study.
Atomic force microscopy has been used for direct visualization of the wrapping of DNA around 30-nm-sized functionalized gold nanoparticles for the first time. The morphology of the complexes seems to be dictated by the relative concentration of the nanoparticles and DNA. A higher concentration of the former leads to the formation of a network of nanoparticles assembled on DNA. This assembly pattern seems to be significantly different from the manner in which cationically modified gold nanoparticles of smaller size (< 5 nm) arrange linearly on DNA, as shown in the literature. A DNA-gold nanoparticle can be developed as a model system for in vitro studies on the mechanism of DNA condensation and also for developing novel methods of nanoparticle self-assembly on the DNA template.
Munia
M
Ganguli
J Venkatesh
JV
Babu
Souvik
S
Maiti
D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010957:Plasmids / Q000737:chemistry / Q000648:ultrastructure; D013057:Spectrum Analysis
D002412:Cations; D006046:Gold; D004247:DNA