Influence of adjuvant formulation on the induced protection of mice immunized with total soluble antigen of Trichinella spiralis.
Vaccination of pigs against the helminth nematode Trichinella could be a good alternative to prevent the risk of human infection. In order to develop an efficient and safe vaccine, the choice of the adjuvant is an important issue. In this study, two adjuvants were selected to prepare vaccines based on total soluble Trichinella spiralis muscle larvae (ML) antigen: Montanide ISA 70 water in oil emulsion and Montanide IMS nanoparticles. Aluminium hydroxide was used as a reference adjuvant. The immune response was checked by ELISA of parasite antigen specific IgG1 and IgE. Finally, protection induced in vaccinated mice was measured after a T. spiralis challenge by counting ML burdens. The results clearly showed an impact of adjuvants on the specific IgG1 and IgE antibody responses against T. spiralis. Differences were observed between the rates of protection induced according to the type of formulation, although the three adjuvants tested were able to enhance the humoral immune response. This work demonstrated the need to use an adjuvant to obtain a specific IgG1 and IgE responses directed against the total soluble extract of T. spiralis.
Deville
Sébastien
S
SEPPIC, 75 Quai d'Orsay, 75321 Paris Cedex 07, France. sebastien.deville@airliquide.com
Pooter
Ambre de
Ad
Aucouturier
Jérôme
J
Lainé-Prade
Véronique
V
Cote
Martine
M
Boireau
Pascal
P
Vallée
Isabelle
I
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D016207:Cytokines / Q000097:blood; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007700:Kinetics; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology; D049329:Nanostructures / Q000737:chemistry; D013047:Specific Pathogen-Free Organisms; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D016207:Cytokines; D004655:Emulsions; D007074:Immunoglobulin G; D007073:Immunoglobulin E