Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries.
Non-typeable isolates of Streptococcus pneumoniae collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, multilocus sequence typing of housekeeping genes, amplification of virulence-related genes, 16S rDNA-RsaI digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of recP and xpt from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and lytA sequences from typical S. pneumoniae strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of S. pneumoniae. These atypical strains may belong to different bacterial species from S. pneumoniae.
Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Republic of Korea.
Kwan Soo
KS
Ko
Won Sup
WS
Oh
Kyong Ran
KR
Peck
Jang Ho
JH
Lee
Nam Yong
NY
Lee
Jae-Hoon
JH
Song
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001208:Asia; D001647:Bile Acids and Salts / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000382:microbiology*; D011803:Quinine / Q000031:analogs & derivatives / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics
D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D037521:Virulence Factors; C017303:ethylhydrocupreine; D011803:Quinine