Sequences of clustered epitopes in Gag and Nef potentially presented by predominant class I human leukocyte antigen (HLA) alleles A and B expressed by human immunodeficiency virus type 1 (HIV-1)-infected patients in Vietnam.
The aim of this study was to define pluriepitopic regions in Gag and Nef possibly relevant in the perspective of a vaccine design in a vietnamese population. The protein sequences derived from gag and nef genes and phenotyping of the class I human leukocyte antigens (HLA) A and B alleles were established for 28 human immunodeficiency virus type 1 (HIV-1)-infected patients from Ho Chi Minh City, Vietnam. The protein sequences display polymorphism mutations as compared with a B reference strain (HXB2). The most frequently represented HLA-A and -B alleles were HLA-A11, A02, and A33 expressed by 35.7, 23.2, and 21.4% of the patients, respectively, and HLA-B75, B46, and B62 expressed by 35.7, 25, and 17.9% of the patients, respectively. This study allows us to determine four pluriepitopic regions in Gag and Nef that should be chosen for a vaccine design in a Vietnamese population.
Lazaro
Estibaliz
E
Laboratoire de Virologie (EA 2968), Université Victor Segalen-Bordeaux 2, Bordeaux, France.
Theodorou
Ioannis
I
Legrand
Elisabeth
E
Recordon-Pinson
Patricia
P
Boucher
Sebastien
S
Capoulade
Corinne
C
Lan
Thi Hoang
TH
Hung
Pham Van
PV
Debre
Patrice
P
Fleury
Herve
H
D016915:AIDS Vaccines; D000483:Alleles; D000595:Amino Acid Sequence; D015195:Drug Design; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015693:Gene Products, nef / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology; D015497:HIV-1; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic; D014744:Vietnam; D054311:nef Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag; D015693:Gene Products, nef; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D054311:nef Gene Products, Human Immunodeficiency Virus