Humoral immune response elicited by plasmid DNA containing HGV E2 gene fragment.
In order to study the feasibility of E2 gene fragment of hepatitis virus G(HGV) as a component of DNA vaccine against the hepatitis virus G infection, a 559bp DNA fragment encoding HGV E2 was cloned into plasmid pCMV-S from pThioHis-E2 in the same reading frame with HBsAg gene to form a recombinant plasmid named pCMV-S-E2. BALB/c mice of Kunming strain were immunized with purified plasmid DNA of pCMV-S-E2 by intra-muscularly inoculation. The immunizations were boosted twice at an interval of 14 days. The whole blood was collected from mice orbit on the day-8 after the last boost. Mice sera were screened by ELISA to determine the humoral immune response using E2-GST fusion protein as the immobilized antigen and the sera from mice immunized with pCMV-S as control. The result indicated that the immunization with plasmid DNA of pCMV-S-E2 could induce quite strong humoral immune response.
College of Pharmacy, Wuhan University, Wuhan 430072, China.
Fethia
F
Ben Yebdri
Abderrahmane
A
Aazaz
Kai
K
Ye
Hui-Wen
HW
Ma
Li-Heng
LH
Tong
D000818:Animals; D005260:Female; D029407:GB virus C / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
D006508:Hepatitis Antibodies; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines; C107619:glycoprotein E2, GB virus C