Induction of G0/G1 arrest and apoptosis by 3-hydroxycinnamic acid in human cervix epithelial carcinoma (HeLa) cells.
The present studies were undertaken to analyze the factors regulating 3-hydroxycinnamic acid-induced apoptosis and cell cycle arrest. Treatment of human cervix HeLa cells with 3-hydroxycinnamic acid induced apoptosis and G0/G1-phase arrest. The percentage of apoptosis induced by 3-hydroxycinnamic acid in HeLa cells was increased with incubation time. The results also demonstrated that 3-hydroxycinnamic acid increased the expression of p53, caspase-3, Bax and cyclin B. These results demonstrated that 3-hydroxycinnamic acid induced apoptosis through p53- and caspase-3-dependent pathways.
Chuang
Jing-Yuan
JY
School of Medical Laboratory Science and Biotechnology,China Medical University, Taiwan, ROC. jgchung@mail.cmu.edu.tw
Tsai
Yueh-Yu
YY
Chen
Ssu-Ching
SC
Hsieh
Tian-Jye
TJ
Chung
Jing-Gung
JG
D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D019926:Cyclin B / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D016193:G1 Phase / Q000187:drug effects*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D003373:Coumaric Acids; D019926:Cyclin B; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases