Characterization of a novel P[25],G11 human group a rotavirus.
A novel rotavirus strain (Dhaka6) isolated from a 21-year-old Bangladeshi male patient was characterized by sequence analysis of its VP7 and VP4 gene segments. Phylogenetic analysis of the VP7 gene of the Dhaka6 strain revealed a common evolutionary lineage with porcine G11 rotavirus strains. This isolate is the first reported G11 rotavirus strain infecting a human host. Comparison of the VP4 gene sequences with all currently recognized 24 different P genotypes revealed only low nucleotide (54 to 71%) and amino acid (52 to 76%) sequence identities. This lack of high sequence similarity in the VP4 gene indicates that the Dhaka6 isolate represents a new group A rotavirus P genotype, to which we propose assignment of the designation P[25].
ICDDR,B, Centre for Health and Population Research, Mohakhali, Dhaka-1212, Bangladesh.
Mustafizur
M
Rahman
Jelle
J
Matthijnssens
Sharifun
S
Nahar
Goutam
G
Podder
David A
DA
Sack
Tasnim
T
Azim
Marc
M
Van Ranst
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus