Retrovirally transduced NCAM140 facilitates neuronal fate choice of hippocampal progenitor cells.
Neural cell adhesion molecule (NCAM) influences proliferation and differentiation of neuronal cells. However, only a little is known about the downstream effects of NCAM signalling, such as alterations in gene transcription, which are associated with cell fate choice. To examine whether NCAM plays a role in cell fate choice during hippocampal neurogenesis, we performed a gain-of-function study, using a retroviral vector which contained full-length NCAM140 cDNA and the marker gene EGFP, and found that NCAM140 promoted neurogenesis by activating proneural transcription activators with concurrent inhibition of gliogenesis. The enhanced transcript levels of proneural transcription factors in NCAM140-transduced cells were down-regulated by treatment of the cells with mitogen-activated protein kinase kinase (MEK) inhibitor PD098059. Overall, these findings suggest that NCAM140 may facilitate hippocampal neurogenesis via regulation of proneurogenic transcription factors in an extracellular signal-regulated kinase (ERK)-dependent manner.
Department of Bicohemistry, Hanyang University College of Medicine, Seoul, Korea.
Ju Hee
JH
Kim
Jung-Ha
JH
Lee
Jin-Yong
JY
Park
Chang-Hwan
CH
Park
Chae-Ok
CO
Yun
Sang-Hun
SH
Lee
Yong-Sung
YS
Lee
Hyeon
H
Son
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004347:Drug Interactions; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000821:virology; D011247:Pregnancy; D020033:Protein Isoforms / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology* / Q000821:virology; D014161:Transduction, Genetic / Q000379:methods*; D014404:Tubulin / Q000378:metabolism
D004791:Enzyme Inhibitors; D005419:Flavonoids; D007211:Indoles; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D012794:Sialic Acids; D014404:Tubulin; C103469:polysialyl neural cell adhesion molecule; D049452:Green Fluorescent Proteins; C007293:DAPI; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one