Stomatal patterning and differentiation by synergistic interactions of receptor kinases.
Coordinated spacing and patterning of stomata allow efficient gas exchange between plants and the atmosphere. Here we report that three ERECTA (ER)-family leucine-rich repeat-receptor-like kinases (LRR-RLKs) together control stomatal patterning, with specific family members regulating the specification of stomatal stem cell fate and the differentiation of guard cells. Loss-of-function mutations in all three ER-family genes cause stomatal clustering. Genetic interactions with a known stomatal patterning mutant too many mouths (tmm) revealed stoichiometric epistasis and combination-specific neomorphism. Our findings suggest that the negative regulation of ER-family RLKs by TMM, which is an LRR receptor-like protein, is critical for proper stomatal differentiation.
Shpak
Elena D
ED
Department of Biology, University of Washington, Seattle, WA 98195, USA.
McAbee
Jessica Messmer
JM
Pillitteri
Lynn Jo
LJ
Torii
Keiko U
KU
D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D004843:Epistasis, Genetic; D015870:Gene Expression; D017343:Genes, Plant; D018519:Meristem / Q000166:cytology; D009154:Mutation; D019441:Plant Epidermis / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development; D018515:Plant Leaves / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D029681:Arabidopsis Proteins; D011956:Receptors, Cell Surface; C459090:too many mouths protein, Arabidopsis; D011494:Protein Kinases; C457763:ER protein, Arabidopsis; C502168:ERL1 protein, Arabidopsis; C502169:ERL2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases