[Studies of effects of lipid on rat fetal neural stem cells].
Rat fetal neural stem cells (rFNSCs) was separated from embryo about 14.5-16.5 days, and cultured in DMEM/F12 media with additives and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Effects of lipid on growth and proliferation of rFNSCs was examined by counting the number of neurospheres and incorporation of 3H. The data show that chemical defined lipid improved rFNSCs' growth and cell division. Lipid will be another neural stem cell's culture media's additive.
Li
Xue-ling
XL
Faculty of Life Science,Neimenggu University, Huhhot 010021, China.
Hu
Ting-mao
TM
Zhalagahu
Yu
Hai-quan
HQ
Morrison
John R
JR