[The genotoxic action of uranyl ions on DNA in vitro caused by the generation of reactive oxygen species].
8-Oxoguanine (8-OG) is an important biomarker of oxidative DNA damage induced by reactive oxygen species (ROS). By using ELISA with monoclonal antibodies against 8-OG, the formation of 8-OG in DNA by the action of uranyl ions, gamma-irradiation, and heating at 37 degrees C and their combined action was investigated in view of environmental pollution by uranium oxides as a result of the use of armor piercing shells with depleted uranium. The content of 8-OG in DNA induced by the action of gamma-irradiation, 5 microM uranyl ions and heating changes with time in a complicated manner. These results suggest that, by the action of uranyl ions, an additional generation of ROS occurs, which leads both to the formation of 8-OG in DNA and its further oxidation. Uranyl ions at a conceptration of 5 microM increase the thermal deamination of cytosine in DNA several times but do not influence DNA thermal depurination. It is shown that uranyl ions essentially increase the production of hydrogen peroxide and hydroxyl radicals by the action of heat on water. The results indicate a high chemical genotoxicity of uranyl ions and their enhancing effect on DNA base damage by the action of heat and gamma-irradiation.
V S
VS
Smirnova
S V
SV
Gudkov
I N
IN
Shtarkman
A V
AV
Chernikov
V I
VI
Bruskov
D000818:Animals; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012474:Salmon
D009153:Mutagens; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; C005460:uranyl acetate; D004247:DNA