Age-dependent change of uric acid level in the dermis using cutaneous microdialysis.
BACKGROUND
We had proposed the usefulness of cutaneous microdialysis for the study of antioxidants in the skin.


OBJECTIVE
We designed a study analyzing the level of uric acid in the skin, one of the major antioxidants, for an age-dependent change.


METHODS
16 healthy male volunteers were divided into two groups according to age. Eleven subjects were in their 3rd decade, under 30 years of age (young group) and the others were their 8th decade (old group), over 70 years of age. Dialysate samples were analyzed using high-performance liquid chromatography analysis.


RESULTS
In the young group the mean level of uric acid was 31.9+/-16.1 microg/ml, while in old group it was 13.4+/-5.2 microg/ml.


CONCLUSIONS
This result demonstrated an in vivo state of antioxidant level in the human skin and the age-dependent difference was concordant with other in vitro or ex vivo studies; therefore, cutaneous microdialysis could be used in analysis and monitoring studies including human antioxidants and anti-aging.
Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
Yong Suk
YS
Lee
Jeong Hoon
JH
Yang
Jung Chul
JC
Choi
Hee Chul
HC
Eun
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D000975:Antioxidants / Q000502:physiology; D020405:Dermis / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008297:Male; D017551:Microdialysis / Q000379:methods*; D014527:Uric Acid / Q000032:analysis
D000975:Antioxidants; D014527:Uric Acid