Isolation and structure determination of bioactive isoflavones from callus culture of Dipteryx odorata.
Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4',6-dimethoxyisoflavone and 3',7-dihydroxy-4',6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4',6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4',6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP, São Paulo, Brazil. ajanuari@unaerp.br
Ana Helena
AH
Januário
Miriam Verginia
MV
Lourenço
Lilian Aparecida
LA
Domézio
Rosemeire Cristina Linhari Rodrigues
RC
Pietro
Marcelo Santos
MS
Castilho
Daniela Maria
DM
Tomazela
Maria Fátima das Graças Fernandes
MF
da Silva
Paulo Cezar
PC
Vieira
João Batista
JB
Fernandes
Suzelei de Castro
Sde C
França
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D046914:Dipteryx / Q000737:chemistry*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D014349:Trypanosoma cruzi / Q000201:enzymology
D007529:Isoflavones; D011994:Recombinant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases