Sequence analysis and structural prediction of the severe acute respiratory syndrome coronavirus nsp5.
The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5. In this study, bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5. Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP). The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins. The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study provided the foundation for further exploration of the protein's biological function, and contributed to the search for anti-SARS-CoV drugs.
Lu
Jia-Hai
JH
The Public Health School of Sun Yat-Sen University, Guangzhou 510080, China. jiahailu@yahoo.com.cn
Zhang
Ding-Mei
DM
Wang
Guo-Ling
GL
Guo
Zhong-Min
ZM
Li
Juan
J
Tan
Bing-Yan
BY
Ou-Yang
Li-Ping
LP
Ling
Wen-Hua
WH
Yu
Xin-Bing
XB
Zhong
Nan-Shan
NS
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D008875:Middle Aged; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000737:chemistry*
D017361:Viral Nonstructural Proteins