Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339.
Triglyceride (TG), a water-insoluble energy-rich lipid, is secreted by the liver as part of very low density lipoproteins (VLDLs) to supply energy to extrahepatic tissues. Overproduction of VLDL is associated with increased risk of cardiovascular heart disease; this has renewed an interest in factors that affect hepatic TG production. The TG production rate is determined by measuring temporal increases in plasma TG under conditions in which TG hydrolysis by lipoprotein lipase (LPL) is inhibited. The nonionic detergent, Triton WR-1339 (Triton), has commonly been used to inhibit LPL for this purpose. Triton, in addition to inhibition of TG hydrolysis, has properties that have the potential to adversely influence lipoprotein metabolism. Another nonionic detergent, poloxamer 407 (P-407), also inhibits LPL. In these studies, we demonstrate that P-407 is comparable to Triton in the determination of TG production but without the unwanted side effects of Triton.
Millar
John S
JS
Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USA.
Cromley
Debra A
DA
McCoy
Mary G
MG
Rader
Daniel J
DJ
Billheimer
Jeffrey T
JT
D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020442:Poloxamer / Q000493:pharmacokinetics / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000493:pharmacokinetics / Q000494:pharmacology*; D011973:Receptors, LDL / Q000172:deficiency; D013501:Surface-Active Agents; D014280:Triglycerides / Q000096:biosynthesis* / Q000097:blood
D001057:Apolipoproteins E; D004791:Enzyme Inhibitors; D011973:Receptors, LDL; D013501:Surface-Active Agents; D014280:Triglycerides; D020442:Poloxamer; D011092:Polyethylene Glycols; D008071:Lipoprotein Lipase; C016811:tyloxapol