Negative regulatory role of mannose receptors on human alveolar macrophage proinflammatory cytokine release in vitro.
Alveolar macrophages (AM) are critical components of lung innate immunity and contribute to an effective host response to Pneumocystis pneumonia. Recognition of unopsonized Pneumocystis organisms by human AM is mediated predominantly via mannose receptors and results in phagocytosis, release of reactive oxygen species, and activation of the nuclear transcription factor (NF)-kappaB. However, the AM host defense genes activated by Pneumocystis have not been defined. In the present study, incubation of AM with unopsonized Pneumocystis organisms was not associated with release of interleukin (IL)-1beta, IL-6, or tumor necrosis factor (TNF)-alpha (important cytokines in the host response to Pneumocystis) and did not induce IL-1beta, IL-6, or TNF-alpha mRNA transcripts. These findings were not attributed to Pneumocystis-induced cytopathic changes, as these same AM released IL-8 and matrix metalloproteinase-9 in response to Pneumocystis. NF-kappaB-mediated IL-8 release was independent of Pneumocystis phagocytosis. The observed response was specific, as IL-1beta, IL-6, and TNF-alpha release and mRNA induction were preserved in response to lipopolysaccharide or serum-opsonized Pneumocystis. The absence of IL-1beta, IL-6, and TNF-alpha release in response to Pneumocystis was predominately influenced by AM mannose receptors, as blocking mannose receptors or targeted mannose receptor small interfering RNA functional gene silencing resulted in TNF-alpha release in response to unopsonized Pneumocystis organisms. Furthermore, ligation of AM mannose receptors by unopsonized Pneumocystis organisms reduced Toll-like receptor 4-mediated TNF-alpha release. Taken together, these data suggest that mannose receptors on human AM may suppress select proinflammatory cytokine release and may serve to regulate the innate inflammatory responses to infectious challenge in the lungs.
Zhang
Jianmin
J
Critical Care and Sleep Medicine, Kirstein Hall, Room E/KSB-23, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA.
Tachado
Souvenir D
SD
Patel
Naimish
N
Zhu
Jinping
J
Imrich
Amy
A
Manfruelli
Pascal
P
Cushion
Melanie
M
Kinane
T Bernard
TB
Koziel
Henry
H
D000293:Adolescent; D000328:Adult; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D025461:Feedback, Physiological / Q000276:immunology; D005260:Female; D020868:Gene Silencing; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis / Q000276:immunology; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000276:immunology*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000276:immunology; D008875:Middle Aged; D016328:NF-kappa B / Q000276:immunology; D011010:Pneumocystis / Q000276:immunology; D011650:Pulmonary Alveoli / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D016209:Interleukin-8; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D016328:NF-kappa B; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D020780:Matrix Metalloproteinase 9