Applicability of oral fluid collected onto filter paper for detection and genetic characterization of measles virus strains.
Expansion of measles molecular surveillance to developing countries where measles is endemic will help facilitate measles control. Limited infrastructure in these areas is a barrier to referral of specimens suitable for measles virus (MV) genotyping. In this study, we demonstrate that oral fluid dried onto filter paper can be used for the detection and characterization of MV strains. Using this approach, an MV-positive sample by reverse transcriptase PCR could be obtained from 67% of serologically confirmed acute measles cases. Mimicking certain environmental conditions and duration of transportation established that MV RNA remained detectable and suitable for nucleic acid sequencing in oral fluid spots for at least 1 week. In the context of a measles outbreak in a remote region of the world where infrastructure is poor, oral fluid samples dried onto filter paper and sent to a specialized laboratory for testing will aid in the identification and characterization of the causative MV strain.
Chibo
Doris
D
WHO Regional Measles Reference Laboratory for the Western Pacific Region, Victorian Infectious Diseases Reference Laboratory, North Melbourne, 3051 Victoria, Australia. Doris.chibo@mh.org.au
Riddell
Michaela A
MA
Catton
Mike G
MG
Birch
Chris J
CJ
D005374:Filtration; D006801:Humans; D008457:Measles / Q000175:diagnosis* / Q000821:virology; D008459:Measles virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000821:virology*; D013048:Specimen Handling / Q000379:methods*
D012367:RNA, Viral