[Preparation of a dry red blood cell Trichinella antigenic diagnosticum for indirect hemagglutination test and evaluation of its efficiency].
The present paper presents the stages of a process for manufacturing a dry red blood cell Trichinella antigenic diagnosticum for indirect hemagglutination test (IHAT) and the evaluation of its diagnostic efficiency. The diagnosticum is shown to be a 3% suspension lyophilized from formalinized and tanned sheep red blood cells on which an excretory-secretory antigen of invasion Trichinella larvae was absorbed in a dose of 100 mg/ml. The rather high sensitivity (98.7%) and specificity (97.3%) of IHAT using the Trichinella diagnosticum based on the excretory-secretory antigen allow it to be considered an effective method for diagnosis of human trichinosis.
Tverdokhlebova
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Vaserin
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D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D004912:Erythrocytes / Q000469:parasitology; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006801:Humans; D051381:Rats; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012756:Sheep; D017160:Trichinella spiralis / Q000276:immunology / Q000302:isolation & purification*; D014235:Trichinellosis / Q000175:diagnosis*
D000947:Antigens, Helminth; D011933:Reagent Kits, Diagnostic