Radioprotective property of the ethanolic extract of Piper betel Leaf.
The radioprotective activity of Piper betel ethanolic extract (PE) has been studied using rat liver mitochondria and pBR 322 plasmid DNA as two model in vitro systems. The extract effectively prevented gamma-ray induced lipid peroxidation as assessed by measuring thiobarbituric acid reactive substrates, lipid hydroperoxide and conjugated diene. Likewise, it prevented radiation-induced DNA strand breaks in a concentration dependent manner. The radioprotective activity of PE could be attributed to its hydroxyl and superoxide radicals scavenging property along with its lymphoproliferative activity. The radical scavenging capacity of PE was primarily due to its constituent phenolics, which were isolated and identified as chevibetol and allyl pyrocatechol.
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic medical Sciences, Kolkata, India.
Sayanti
S
Bhattacharya
Mahesh
M
Subramanian
Susri
S
Roychowdhury
Ajay K
AK
Bauri
Jaya P
JP
Kamat
Subrata
S
Chattopadhyay
Sandip Kumar
SK
Bandyopadhyay
D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D000431:Ethanol / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000528:radiation effects*; D031721:Piper betle / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010957:Plasmids / Q000187:drug effects* / Q000528:radiation effects*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D011837:Radiation-Protective Agents; D000431:Ethanol