Chitinases from Serratia marcescens Nima.
Chitinolytic activity of Serratia marcescens Nima (130 U ml(-1)) was up to 43 times higher than those produced by other S. marcescens strains. This strain synthesized an endochitinase (Chi-60), an exochitinase (Chi-50) and a novel N-acetylglucosaminidase. This latter showed two putative isoforms (Chi-180.5 and Chi-180.8) with isoelectric points of 5 and 8.1, respectively.
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, 11340 México.
A
A
Ruiz-Sánchez
R
R
Cruz-Camarillo
R
R
Salcedo-Hernández
J E
JE
Barboza-Corona
D000118:Acetylglucosaminidase / Q000737:chemistry* / Q000235:genetics; D001413:Bacillus thuringiensis / Q000201:enzymology; D001709:Biotechnology / Q000379:methods*; D002688:Chitinases / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D020033:Protein Isoforms; D012706:Serratia marcescens / Q000201:enzymology*
D006023:Glycoproteins; D020033:Protein Isoforms; D006026:Glycoside Hydrolases; D002688:Chitinases; D000118:Acetylglucosaminidase