The interface behavior and biocatalytic activity of superoxide dismutase at carbon nanotube.
The interface behavior and biocatalytic activity of superoxide dismutase (SOD) were studied at carbon nanotube (CNT) surface with cyclic voltammetry (CV). The results show that SOD participates in a rapid exchange with CNT and the process is a single-electron-single-proton process. The electron-transfer coefficient was calculated to be 0.52, the electron-transfer rate constant was 1.4s(-1) and the average surface coverage was measured to be 6.93 x 10(-11)+/-4.2 x 10(-12) mol cm(-2). The bioactive measurements show that SOD keeps its bioactivity at the CNT surface. SOD's remarkable ability to catalyze the dismutation of the superoxide anion (O (2)(-)) has been demonstrated.
Key Laboratory of Biomedical Photonics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, HuBei 430074, PR China.
Yan-Hua
YH
Bi
Zhen-Li
ZL
Huang
Yuan-Di
YD
Zhao
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002384:Catalysis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000737:chemistry*; D013499:Surface Properties
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D013482:Superoxide Dismutase; D010100:Oxygen