Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects.
Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.
Wang
Jimin
J
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520-8114, USA. wang@mail.csb.yale.edu
Rho
Seong Hwan
SH
Park
Hyun Ho
HH
Eom
Soo Hyun
SH
D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D049071:Endopeptidase Clp / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D012697:Serine Endopeptidases / Q000737:chemistry*
C483566:ClpYQ protease, E coli; D029968:Escherichia coli Proteins; C423319:CodWX protease; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp