Transfection of Mülller cells with type I interferon transgenes: resistance to HSV-1 infection.
Gene transfer is a widely used experimental approach to determine the value of specific genes under a variety of conditions. This chapter focuses on the expression of two human anti-viral genes, interferon (IFN)-alpha2 and IFN-beta, driven by a cytomegalovirus immediate-early promoter in a human Müller cell line. The anti-viral efficacy of these two transgenes is determined by measuring resistant to herpes simplex virus type 1 infection assessing viral antigen expression and viral titers recovered from control and IFN transgene-transfected cells. Furthermore, comparing the antiviral efficacy of these two transgenes suggest the anti-viral environment elicited by the human IFN-alpha2 transgene is superior to that of the human IFN-beta, which may be related to the amount of biologically active IFN secreted by these cells.
John-Philip
Benitta
B
Department of Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, USA.
Carr
Daniel J J
DJ
D000818:Animals; D002460:Cell Line; D018014:Gene Transfer Techniques; D006561:Herpes Simplex / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000235:genetics* / Q000276:immunology; D012160:Retina / Q000166:cytology*; D014162:Transfection; D019076:Transgenes
D007370:Interferon Type I