Evaluation of the anti-Trichophyton activity of a prodigiosin analogue produced by gamma-proteobacterium, using stratum corneum epidermis of the Yucatan micropig.
Prodigiosins (PGs) are known to be a family of natural red pigments, characterized by a common pyrrolydipyrrolylmethane skeleton structure with a C-4 methoxy group, and some of these pigments have been isolated from some microorganisms. Members of the PG family have been reported to show several biological activities, such as immunosuppressive and cytotoxic activities. Recently, we discovered a bacterial strain (MS-02-063), from our microbial library, that produces large amounts of a PG analogue (PG-L-1). In this study, we examined the anti-Trichophyton activity of PG-L-1 (produced by strain MS-02-063) against clinically isolated Trichophyton spp., by a method using stratum corneum epidermis (SCE) of the Yucatan micropig, which is suitable for estimating the antifungal activity of drugs in vitro. In the National Committee for Clinical Laboratory Standards (NCCLS) method, PG-L-1 showed potent antifungal activity against nine clinically isolated strains of Trichophyton spp., although the minimum inhibitory concentration (MIC) values were slightly higher than those of bifonazole. In spite of the lower efficiency of PG-L-1 transfer into SCE from medium than that of bifonazole, PG-L-1 transferred into SCE showed more potent antifungal activity than bifonazole, at lower concentrations.
Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Nagasaki, Japan. takuji.nakashima@nifty.com
Takuji
T
Nakashima
Yoko
Y
Kato
Kenichi
K
Yamaguchi
Tatsuya
T
Oda
D000818:Animals; D004353:Drug Evaluation, Preclinical; D004817:Epidermis; D020563:Gammaproteobacteria / Q000378:metabolism*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013552:Swine; D013556:Swine, Miniature; D014008:Tinea Pedis / Q000382:microbiology; D046509:Tissue Culture Techniques; D014249:Trichophyton / Q000187:drug effects*
D011353:Prodigiosin