[Study of Five STR Loci in A Chinese Han Population.].
To understanding the allele structure and genetic polymorphisms at five STR loci in Chinese Han population, and construct a preliminary database, EDTA-blood specimens were collected from unrelated individuals. DNA samples were extracted with Chelex method and were amplified by PCR technique. The PCR products were analyzed using both PAGE horizontal electrophoresis with discontinuous buffer system and automated fluorescence detection approach. As a result, three STRs consist of simple repeat motifs, while two STR contain a complex repeat structure. The STR polymorphisms at all of the five loci have been observed in Chinese Han population. In a word, the obtained data are beneficial to understanding the population genetics of the five STR loci in Chinese Han population. As a simple approach, the PAGE horizontal electrophoresis can be employed for typing the five STR markers.
Jia
Zhen-Jun
ZJ
School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wu
Jin
J
Li
Hui
H
Hou
Yiping
Y
Zhang
Wei-Juan
WJ
Zhou
Xue-Ping
XP
Deng
Jian-Qiang
JQ
Shi
Mei-Lin
ML
Zhang
Ji
J
Li
Ying-Bi
YB
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D005828:Genetics, Population; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences