title: The efficacy of a subcutaneous injection of soluble Vitamin B12 in lambs.
abstract: OBJECTIVE
  To assess the efficacy of a soluble Vitamin B12 injection in lambs by measuring changes in the serum and liver Vitamin B12 concentrations.
  
  
  METHODS
  Thirty-six lambs were injected subcutaneously with 2 mg of soluble Vitamin B12 while another group of 36 served as untreated controls. Blood and liver biopsy samples for Vitamin B12 determinations were collected just before the injection and at days 1, 2, 5, 8, 16, 24, 30 and 45.
  
  
  RESULTS
  The serum Vitamin B12 concentrations of the Vitamin B12 treated lambs increased rapidly compared to the untreated lambs. Concentrations peaked at day 2, decreased rapidly to day 8, and then decreased more slowly until day 24 when there were no longer differences between the groups. Liver Vitamin B12 concentrations of the Vitamin B12 treated lambs were significantly greater over days 8-24.
  
  
  CONCLUSIONS
  A subcutaneous injection of 2 mg of soluble Vitamin B12 was effective in increasing and maintaining the Vitamin B12 status of lambs for about 24 days.
  
  
  CONCLUSIONS
  This Vitamin B12 product is only effective for preventing cobalt deficiency in lambs for about 4 weeks.
authors: Dairy and Beef Division, AgResearch Grasslands, Private Bag 11008, Palmerston North, New Zealand.
authors: N D
authors: ND
authors: Grace
authors: D M
authors: DM
authors: West
authors: N D
authors: ND
authors: Sargison