title: [Effects of different factors on direct induction of microtubers from explants in Pinellia ternate].
abstract: OBJECTIVE
  To study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose.
  
  
  METHODS
  Using the orthogonal design method.
  
  
  CONCLUSIONS
  The optimal media to directly induce microtubers from leaves were MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3% and MS+ 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + sucrose 3%. Optimal media to directly induce microtubers from tubers were MS + 6-BA 1.0 mg x L(-1) + PEG 5% + sucrose 5% and MS+ CH 500 mg x L(-1) + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + AC 0.5% + sucrose 5%. Media suitable for plantlet growth were MS + 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + AC 0.5% + sucrose 5% and MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3%.
authors: Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
authors: Ai-Min
authors: AM
authors: Zhang
authors: Sheng-Yu
authors: SY
authors: Yang
authors: Jian-Ping
authors: JP
authors: Xue
authors: Wei
authors: W
authors: Sheng
authors: Xiang
authors: X
authors: Gao
authors: Yan-Fang
authors: YF
authors: Zhu
mesh_terms: D002244:Carbon / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005947:Glucose / Q000494:pharmacology; D030021:Pinellia / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000254:growth & development; D035281:Plant Tubers / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D002364:Caseins; D003470:Culture Media; D010937:Plant Growth Regulators; D011092:Polyethylene Glycols; D013395:Sucrose; C475729:casein hydrolysate; D002244:Carbon; D005947:Glucose