title: The wheat TaGI1, involved in photoperiodic flowering, encodes an Arabidopsis GI ortholog.
abstract: Wheat (Triticum aestivum L.) is an important crop and requires long day and short night to flower. To study the molecular mechanism of photoperiodic regulation of flowering in this species, we isolated a wheat TaGI1 gene, an ortholog of GIGANTEA (GI) in Arabidopsis. RNA blot hybridization revealed that TaGI1 is expressed in leaves in a rhythmic manner under long day and short day conditions and its rhythmic expression is regulated by photoperiods and circadian clocks. Further study demonstrated that the TaGI1 rhythmic expression in the leaves of seedlings is initiated by photoperiods, implying that TaGI1 does not show circadian regulation until after being entrained in a light/dark cycle. Interestingly, TaGI1 mRNA was detected in adaxial epidermal cells right above the vascular bundles of leaves, suggesting that the localization of TaGI1 transcripts in leaves may function to regulate flowering in response to photoperiods. Since overexpression of TaGI1 altered flowering time in wild type and complemented the gi mutant in Arabidopsis, it confirmed that TaGI1 is an ortholog of GI in Arabidopsis.
authors: Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, China.
authors: Xiang Yu
authors: XY
authors: Zhao
authors: Mao Sen
authors: MS
authors: Liu
authors: Jia Rui
authors: JR
authors: Li
authors: Chun Mei
authors: CM
authors: Guan
authors: Xian Sheng
authors: XS
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics; D015152:Blotting, Northern; D002940:Circadian Rhythm; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D019697:Onions / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D017440:Photoperiod; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C121183:GI protein, Arabidopsis; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins