A very short segment of the murine Ret promoter contains elements sensitive to in vitro neural cell differentiation.
Regulation of the murine Ret gene has only been partially studied, specifically cis-acting promoter elements and related transcription factors, although in vivo experiments have shown the ability of Ret 5' flanking sequences to drive expression of transgenic reporter genes. This work describes the characterization of a 387-bp fragment of the Ret 5' flanking sequence with the ability to activate expression of the LacZ gene in transfected cells, in which SP1 recognition elements played a fundamental role. Using P19 teratocarcinoma cells differentiated by retinoic acid treatment, we observed an induction of Ret mRNA accompanied by a decrease of SP1 binding due to its proteolytic cleavage.
Zordan
Paola
P
Laboratory of Molecular Genetics, G. Gaslini Institute, Largo G. Gaslini 5, 16147 Genova, Italy.
Ravazzolo
Roberto
R
Bocciardi
Renata
R
D024506:5' Flanking Region / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology; D009841:Oligonucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014162:Transfection
D009841:Oligonucleotides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D008156:Luciferases; D051096:Proto-Oncogene Proteins c-ret; C494999:Ret protein, mouse