Mutations in the gyrA and parC genes in ciprofloxacin-resistant clinical isolates of Acinetobacter baumannii in Korea.
Mutation with Ser-83-->Leu in gyrA gene was associated with the principal mutation for ciprofloxacin resistance in clinical isolates of Acinetobacter baumannii. Double mutation, Ser-83-->Leu in gyrA gene and Ser-80-->Leu in parC gene, was the most frequently detected among ciprofloxacin-resistant isolates. A novel mutation with Ser-80-->Trp in parC gene, in addition to mutation in gyrA gene, was associated with a high-level ciprofloxacin resistance. These results suggested that the presence of an additional mutation in the parC gene contributed to a higher-level of ciprofloxacin resistance than a single mutation in the gyrA gene (geometric mean MICs of ciprofloxacin, 44.1 versus 16 microg/ml, P < 0.05).
Lee
Jeom Kyu
JK
Division of Antimicrobial Resistance Pathogens, Department of Bacteriology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul.
Lee
Yeong Seon
YS
Park
Yong Keun
YK
Kim
Bong Su
BS
D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010802:Phylogeny
D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase