[Expressions and role of endogenetic matrix metalloproteinases-9 and transforming growth factor-beta during wound healing of blast injury].
OBJECTIVE
To investigate the expression of endogenetic matrix metalloproteinases-9 (MMP-9) and transforming growth factor-beta(TGF-beta) and their role in the wound healing of blast injury.


METHODS
Rat models of blast injury under a humid and hot environment were established and the effusion from the wound surface was collected at 4, 24, 48 h and 5, 7, 14, 21 and 28 days after injury, respectively. The contents of MMP-9 and TGF-beta in the effusion of the wound were measured by zymography and enzyme-linked immunosorbent assay (ELISA), respectively.


RESULTS
During the wound healing of blast injury, MMP-9 and TGF-beta exhibited changes that followed a regular pattern, both reaching the peak value at 48 h after the injury. TGF-beta content reached the another peak on day 7. TGF-beta value and MMP-9 contents decreased in the second week after injury and their reduction was no longer parallel. Administration of tissue inhibitor of metalloproteinase (TIMP) in the early phase of injury showed no obvious effect, but during the 2 weeks after the injury, its administration caused decrease in MMP-9 content and increase in TGF-beta content in the effusion.


CONCLUSIONS
In the early phase of wound healing, the elevation of MMP-9 and TGF-beta accelerated cell migration to promote the clearance of the inflammatory necrosis tissues, which might be one of the wound healing mechanisms. But overexpression of MMP-9 in the wound may hinder wound healing, and appropriate use of TIMP can accelerate the delayed wound healing.
Zhu
Xin-yong
XY
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zxy517710@sohu.com
Fang
Chi-hua
CH
Zhang
Li-yun
LY
Zuo
Da-ming
DM
Chen
Zheng-liang
ZL
D000818:Animals; D001753:Blast Injuries / Q000378:metabolism*; D005260:Female; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014945:Wound Healing
D016212:Transforming Growth Factor beta; D020780:Matrix Metalloproteinase 9