Functionalized head-to-head hairpin polyamides: synthesis, double-stranded DNA-binding activity and affinity.
A series of 4 functionalized head-to-head-linked hairpin oligo(N-methylpyrrole) carboxamides with different linkers have been synthesized. Their ability to bind double-stranded DNA and sequence specificity were compared and the apparent Kd values of their DNA complexes were determined. These compounds, particularly those with iminodiacetic linkers, revealed a high affinity for DNA (Kd = 4.5-4.8 x 10(-9) M) and sequence specific recognition of 9-10 base pairs.
Halby
Ludovic
L
USM 0503-Régulation et Dynamique des Génomes, Muséum National d'Histoire Naturelle, Paris, France.
Ryabinin
Vladimir A
VA
Sinyakov
Alexandre N
AN
Boutorine
Alexandre S
AS
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006678:HIV / Q000235:genetics; D006493:Heparin / Q000138:chemical synthesis* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000138:chemical synthesis* / Q000378:metabolism*; D011533:Proviruses / Q000737:chemistry / Q000235:genetics
D009757:Nylons; D006493:Heparin; D004247:DNA