title: Isolation and characterization of simple repeat sequences from the yellow fin sea bream Acanthopagrus latus (Sparidae).
abstract: We isolated DNA fragments containing various repetitive elements from the genome of a sea bream Acanthopagrus latus. Sequence analysis indicated that two fragments have particularly interesting features. Fragment AL87 contained a tetranucleotide repeat and a quasipalindromic sequence. Sequence comparison suggested that AL87 may be a part of a gene encoding a serine/threonine protein kinase, and that the quasipalindrome is situated at the junction of an intron and an exon. Moreover, the quasipalindrome is conserved in several other fishes, even though it has the potential to form a stem-loop structure at the splicing site. Fragment AL79 contained a minisatellite sequence made up of six 30-bp units in tandem. DNase I sensitivity assays and statistical analyses showed the repeat region to be flexible when subjected to bending stress. In addition, atomic force microscopic imaging of AL79 showed the presence of highly curved (kinked) segments flanking the repeat region. The structural features of these repetitive elements may be key factors facilitating the amplification of the repeats.
authors: Department of Life Sciences, Osaka Prefecture University College of Integrated Arts and Sciences, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan.
authors: Gamal E
authors: GE
authors: Elmesiry
authors: Sayaka
authors: S
authors: Okai
authors: Shingo
authors: S
authors: Hokabe
authors: Shinsei
authors: S
authors: Minoshima
authors: Shigeru
authors: S
authors: Sugiyama
authors: Tomoyuki
authors: T
authors: Yoshino
authors: Toshio
authors: T
authors: Ohtani
authors: Nobuyoshi
authors: N
authors: Shimizu
authors: Mikio
authors: M
authors: Kato
mesh_terms: D000818:Animals; D001483:Base Sequence; D003850:Deoxyribonuclease I / Q000378:metabolism; D005819:Genetic Markers; D023281:Genomics; D018895:Microsatellite Repeats; D018625:Microscopy, Atomic Force; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D021541:Sea Bream / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D003850:Deoxyribonuclease I