A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA.
A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly degraded DNA from casework samples, which had shown no or only poor results for these loci in previous analyses with standard primer sets. The application of the new miniSTR-multiplex resulted in an increased overall typing success rate for degraded DNA samples. In a concordance study between the conventional and the newly designed primers, no genotype differences were revealed in 124 randomly selected individuals.
Grubwieser
P
P
Institute of Legal Medicine, Innsbruck Medical University, Müllerstrasse 44, 6020 Innsbruck, Austria.
Mühlmann
R
R
Berger
B
B
Niederstätter
H
H
Pavlic
M
M
Parson
W
W
D053523:Amelogenin / Q000235:genetics; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D003850:Deoxyribonuclease I; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences
D053523:Amelogenin; D017931:DNA Primers; D003850:Deoxyribonuclease I