Inhibition of sortase-mediated Staphylococcus aureus adhesion to fibronectin via fibronectin-binding protein by sortase inhibitors.
The sortase enzymes are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. In Staphylococcus aureus, deletion of the sortase isoforms results in marked reduction in virulence and infection potential, making it an important antivirulence target. Recombinant sortase A (SrtA) and sortase B (SrtB) were incubated with peptide substrate containing either the LPETG or NPQTN motifs. (Z)-3-(2,5-dimethoxyphenyl)-2-(4-methoxyphenyl) acrylonitrile, beta-sitosterol-3-O-glucopyranoside, berberine chloride, and psammaplin A1 showed potent inhibitory activity against SrtA and SrtB. These compounds also exhibited potent inhibitory activity against S. aureus cell adhesion to fibronectin. The fibronectin-binding activity data highlight the potential of these compounds for the treatment of S. aureus infections via inhibition of sortase activity.
Oh
Ki-Bong
KB
School of Agricultural Biotechnology, Seoul National University, San 56-1, Sillim, Gwanak, Seoul 151-921, South Korea. ohkibong@snu.ac.kr
Oh
Mi-Na
MN
Kim
Jae-Gyu
JG
Shin
Dong-Sun
DS
Shin
Jongheon
J
D018829:Adhesins, Bacterial / Q000378:metabolism*; D019881:Aminoacyltransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism*; D015394:Molecular Structure; D011994:Recombinant Proteins; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005353:Fibronectins; D011994:Recombinant Proteins; C078635:fibronectin-binding proteins, bacterial; C509821:sortase B; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases