title: Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping.
abstract: Accurate and comprehensive sequence coverage for large genomes has been restricted to only a few species of specific interest. Lower sequence coverage (survey sequencing) of related species can yield a wealth of information about gene content and putative regulatory elements. But survey sequences lack long-range continuity and provide only a fragmented view of a genome. Here we show the usefulness of combining survey sequencing with dense radiation-hybrid (RH) maps for extracting maximum comparative genome information from model organisms. Based on results from the canine system, we propose that from now on all low-pass sequencing projects should be accompanied by a dense, gene-based RH map-construction effort to extract maximum information from the genome with a marginal extra cost.
authors: CNRS, UMR 6061, Génétique et développement, Faculte de Médecine, Rennes, France.
authors: Christophe
authors: C
authors: Hitte
authors: Jennifer
authors: J
authors: Madeoy
authors: Ewen F
authors: EF
authors: Kirkness
authors: Catherine
authors: C
authors: Priat
authors: Travis D
authors: TD
authors: Lorentzen
authors: Fabrice
authors: F
authors: Senger
authors: Dan
authors: D
authors: Thomas
authors: Thomas
authors: T
authors: Derrien
authors: Christina
authors: C
authors: Ramirez
authors: Carol
authors: C
authors: Scott
authors: Gwenaelle
authors: G
authors: Evanno
authors: Barbara
authors: B
authors: Pullar
authors: Edouard
authors: E
authors: Cadieu
authors: Vinay
authors: V
authors: Oza
authors: Kristelle
authors: K
authors: Lourgant
authors: David B
authors: DB
authors: Jaffe
authors: Sandrine
authors: S
authors: Tacher
authors: Stéphane
authors: S
authors: Dréano
authors: Nadia
authors: N
authors: Berkova
authors: Catherine
authors: C
authors: André
authors: Panagiotis
authors: P
authors: Deloukas
authors: Claire
authors: C
authors: Fraser
authors: Kerstin
authors: K
authors: Lindblad-Toh
authors: Elaine A
authors: EA
authors: Ostrander
authors: Francis
authors: F
authors: Galibert
mesh_terms: D000818:Animals; D004285:Dogs; D006801:Humans; D010802:Phylogeny; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA