title: [DNA and chemical analyses of commercial fly agaric-related products].
abstract: Since June 6, 2002, psilocin and psilocybin-containing fungi (commonly called "magic mushrooms") have been regulated by the Narcotics and Psychotropics Control Law in Japan. However, various fly agaric-related products are now entering the Japanese market via the internet. In this study, fly agaric-related products available in this way were investigated for raw materials by DNA analysis and for additives by chemical analysis. Nucleotide sequence analysis of the mitochondrial 12S rDNA region suggested that these fly agaric-related products originate from A. muscaria or A. muscaria var. persicina. Furthermore, they were classified into three strains based on the ITS2-LSU nucleotide sequence. Harmine derivatives and/or tryptamine derivatives were detected in some of these products by LC/MS analysis. In accordance with this, the matK gene of Peganum harmala was found in all of the harmine derivative-containing samples.
authors: National Institute of Health Sciences: 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors: Takuro
authors: T
authors: Maruyama
authors: Nobuo
authors: N
authors: Kawahara
authors: Toshimitsu
authors: T
authors: Fukiharu
authors: Kazumasa
authors: K
authors: Yokoyama
authors: Yukiko
authors: Y
authors: Makino
authors: Yukihiro
authors: Y
authors: Goda
mesh_terms: D000545:Amanita / Q000737:chemistry*; D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D006247:Harmine / Q000032:analysis; D020407:Internet; D014363:Tryptamines / Q000032:analysis
chemical_list: D004271:DNA, Fungal; D014363:Tryptamines; C030820:tryptamine; D006247:Harmine