Applications of short-coherence digital holography in microscopy.
We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.
Martínez-León
Lluís
L
Departament de Ciències Experimentals, Universitat Jaume I, 12071 Castelló, Spain. lluis.martinez@uji.es
Pedrini
Giancarlo
G
Osten
Wolfgang
W
D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*