Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival.
Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29A(ficP) mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29A(ficP) males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29A(ficP) mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29A(ficP) mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A+ in male genital development and survival.
Hamada
Noriko
N
Laboratory of Genetics, Waseda University, 2-7-5, Higashi-Fushimi, Nishi-Tokyo,Tokyo 202-0021, Japan.
Bäckesjö
Carl-Magnus
CM
Smith
C I Edvard
CI
Yamamoto
Daisuke
D
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D015153:Blotting, Western; D004330:Drosophila / Q000235:genetics*; D005837:Genitalia, Male / Q000196:embryology*; D006801:Humans; D008297:Male; D009154:Mutation; D010641:Phenotype; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D019076:Transgenes
D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human