title: Biphenotypic acute leukemia with t(15;17).
abstract: Biphenotypic acute leukemias (BAL) represent 5% of all acute leukemias. The most frequent cytogenetic abnormalities described are Philadelphia chromosome and 11q23 involvement. Here we report a BAL case, with blasts showing lymphoblast morphology and positivity for myeloperoxidase (in 6% of the blast cells). Immunophenotype revealed the compromise of myeloid and B-lymphoid lineages. Cytogenetic analysis showed the t(15;17) and 8 trisomy. PML/RARa rearrangement was detected by fluorescent in situ hybridization (FISH) on interphase nuclei while PML/RARa fusion transcript was detected in the bone marrow and peripheral blood by molecular biology studies (RT-PCR). This report describes a BAL case with an unfrequent cytogenetic abnormality, and highlights the importance of correlating the results of multiple diagnostic methods in order to establish a correct diagnosis and treatment in BAL patients.
authors: IIHEMA, Academia Nacional de Medicina de Buenos Aires.
authors: M P
authors: MP
authors: Scolnik
authors: P Negri
authors: PN
authors: Aranguren
authors: M T
authors: MT
authors: Cuello
authors: M F
authors: MF
authors: Palacios
authors: J
authors: J
authors: Sanjurjo
authors: M
authors: M
authors: Giunta
authors: M M E
authors: MM
authors: Bracco
authors: S
authors: S
authors: Acevedo
mesh_terms: D000208:Acute Disease; D002648:Child; D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015321:Gene Rearrangement; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D018365:Neoplasm, Residual / Q000175:diagnosis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014314:Trisomy