Intracellular localization and domain organization of human TRIM41 proteins.
A human gene previously identified as a partial cDNA homologous to the gene of RET finger protein was characterized. Northern hybridization detected three messages of 3.3, 4.2, and 7.5 kb. The coding sequences of the more abundant of the three messages, the 4.2 and the 3.3 kb, were determined. The former encodes a 630 amino acid protein (TRIM41alpha) and the latter a 518 amino acid protein (TRIM41beta). Green fluorescent protein (GFP) fusions of full-length TRIM41alpha and TRIM41beta were both observed as speckles in the cytoplasm and the nucleus. The result was corroborated by Western analysis of cellular fractions. Results with GFP fusions of various segments of the TRIM41 proteins indicated that the nuclear transport of the proteins is mediated by an N-terminal segment common to both isoforms, but independent of a classical nuclear localization signal sequence.
Tanaka
Mayumi
M
Department of Life Science and Frontier Project "Life's Adaptation Strategies to Environmental Changes", Rikkyo (St. Paul's) University, Tokyo 171-8501, Japan.
Fukuda
Yoshiko
Y
Mashima
Keisuke
K
Hanai
Ryo
R
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D049452:Green Fluorescent Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D014162:Transfection; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C505879:TRIM41 protein, human; D044767:Ubiquitin-Protein Ligases