The modified ABC model explains the development of the petaloid perianth of Agapanthus praecox ssp. orientalis (Agapanthaceae) flowers.
The class B genes, which belong to the MADS-box gene family, play important roles in regulating the development of petals and stamens in flowering plants. To understand the molecular mechanisms of floral development in Agapanthus praecox ssp. orientalis (Agapanthaceae), we isolated and characterized the homologs of the Antirrhinum majus genes GLOBOSA and DEFICIENS in this plant. These were designated as ApGLO and ApDEF, respectively. ApGLO and ApDEF contain open reading frames that encode deduced protein with 210 and 214 amino acid residues, respectively. Phylogenetic analysis indicated that ApGLO and ApDEF belong to the monocot class B gene family. In situ hybridization experiments revealed that hybridization signals of ApGLO and ApDEF were observed in whorl 1 as well as in whorls 2 and 3. Moreover, the flowers of transgenic Arabidopsis plants that ectopically expressed ApGLO formed petal-like organs in whorl 1. These observations indicate that the flower developmental mechanism of Agapanthus follows the modified ABC model.
Nakamura
Toru
T
Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, 980-8577 Aoba-ku Sendai, Japan. kanno@ige.tohoku.ac.jp
Fukuda
Tatsuya
T
Nakano
Masaru
M
Hasebe
Mitsuyasu
M
Kameya
Toshiaki
T
Kanno
Akira
A
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D026281:DEFICIENS Protein / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D026161:MADS Domain Proteins / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics* / Q000254:growth & development; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D026281:DEFICIENS Protein; D018076:DNA, Complementary; C102172:GLOBOSA protein, plant; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D010940:Plant Proteins; D012333:RNA, Messenger