Identification of a novel HLA-A*02 allele, A*027401*.
A novel HLA-A*02 allele was detected in a Caucasian patient from central Italy, requiring a hematopoietic cell transplantation. Direct sequencing identified a variation in one nucleotide position, which was confirmed by cloning. The name A*027401 was officially assigned by the WHO Nomenclature Committee in November 2004. A*027401 differs from A*02010101 by a single G to A substitution at nucleotide position 595 in exon 3. The new variant would lead to a nonsynonymous nucleotide change (GGG to AGG) at codon 175, resulting in a basic Arg in the alpha-helix of the alpha2-domain, in place of a non-polar Gly. The presence of an uncommon variation at a highly conserved nucleotide position could have implications in unrelated hematopoietic cell transplantation.
Piancatelli
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C.N.R. Institute for Organ Transplantation and Immunocytology, L'Aquila, Italy. d.piancatelli@itoi.cnr.it
Beato
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TD
Papola
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F
Aureli
A
A
Fioroni
A
A
Canossi
A
A
Mastrangelo
E
E
Liberatore
G
G
Tessitore
A
A
Adorno
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D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007558:Italy / Q000208:ethnology; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D044465:White People / Q000235:genetics
D015234:HLA-A Antigens