title: Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate.
abstract: Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of more than one gene cannot be monitored simultaneously by this system because one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay system in which three luciferases that emit green, orange, and red light with a single substrate are used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply and rapidly monitor multiple gene expressions in a one-step reaction.
authors: National Institute of Advanced Industrial Science and Technology, Osaka, Japan.
authors: Yoshihiro
authors: Y
authors: Nakajima
authors: Takuma
authors: T
authors: Kimura
authors: Kazunori
authors: K
authors: Sugata
authors: Toshiteru
authors: T
authors: Enomoto
authors: Atsushi
authors: A
authors: Asakawa
authors: Hidehiro
authors: H
authors: Kubota
authors: Masaaki
authors: M
authors: Ikeda
authors: Yoshihiro
authors: Y
authors: Ohmiya
mesh_terms: D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D008156:Luciferases