[Screening for new binding proteins which interact with BM2 of influenza B virus with yeast two-hybrid system].
OBJECTIVE
To explore the role of BM2 protein in the life cycle of influenza B virus.


METHODS
The authors screened human kidney MATCHMAKER cDNA library for new binding partners of BM2 of influenza B virus by using the yeast two hybrid system with truncated BM2 (26-109 aa) as the bait.


RESULTS
Six positive plasmids encoding N-acetylneuraminate pyruvate lyase, angiopoietin 3, zinc finger protein 251, ribosomal protein S20, protein arginine N-methyltransferase 1 variant 1 (PRMT) and transcription factor-like 1 (TCFL1) were obtained.


CONCLUSIONS
The results suggest that BM2 may play an important role in the life cycle of influenza B virus.
Yu
Hong
H
State Key Laboratory of Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Yao
Li-hong
LH
Chen
Ai-jun
AJ
He
Jie
J
Jia
Run-qing
RQ
Cheng
Cong-sheng
CS
Zhang
Zhi-qing
ZQ
D000091304:Angiopoietin-Like Protein 1; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D009981:Influenza B virus / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics
C117578:ANGPTL1 protein, human; D000091304:Angiopoietin-Like Protein 1; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins; C064964:BM2 polypeptide, influenza B virus; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D012269:Ribosomal Proteins; D014157:Transcription Factors; C092773:VPS72 protein, human; D014764:Viral Proteins; C029611:ribosomal protein S20; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase