Alterations in peripheral blood levels of TIMP-1, MMP-2, and MMP-9 in patients with type-2 diabetes.
OBJECTIVE
In vivo and in vitro experimental findings indicate that the hyperglycemic diabetic milieu can induce altered expression of the matrix metalloproteinase (MMP) genes and contribute to imbalances in vascular matrix homeostasis. We examined the plasma levels of enzymes and inhibitors involved in extracellular matrix turnover.


METHODS
We measured the plasma concentrations of MMP-2, MMP-9, and tissue inhibitor of metalloproteinase 1 (TIMP-1) in 80 type-2 diabetic subjects without uremia and in 80 age-matched controls. In addition, we determined the plasma levels of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and high sensitive(hs) C-reactive protein (CRP) in both groups.


RESULTS
Plasma MMP-2, TIMP-1, and hs-CRP concentrations were significantly elevated in diabetic patients as compared to the control subjects (p<0.05). Plasma levels of MMP-2, MMP-9, TIMP-1, VCAM-1, ICAM-1, and hs-CRP were found not to be significantly associated with age, duration of diabetes, blood pressure, or serum lipid concentrations.


CONCLUSIONS
Plasma MMP-2, TIMP-1 and hs-CRP concentrations were significantly increased in type-2 diabetic patients.
Lee
Seung Won
SW
Department of Endocrinology and Metabolism, Ajou University School of Medicine, San-5 Wonchon Dong, Yongtong Gu,443-721, Suwon, South Korea.
Song
Kyoung Eun
KE
Shin
Dong Sun
DS
Ahn
Sung Min
SM
Ha
Eun Suk
ES
Kim
Dae Jung
DJ
Nam
Moon Suk
MS
Lee
Kwan-Woo
KW
D001794:Blood Pressure; D002097:C-Reactive Protein / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000201:enzymology; D005260:Female; D006706:Homeostasis; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D012016:Reference Values; D012907:Smoking; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*
D008055:Lipids; D019715:Tissue Inhibitor of Metalloproteinase-1; D002097:C-Reactive Protein; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9