Culturing of specialized glial cells (olfactory ensheathing cells) of human olfactory epithelium.
A monolayer of dissociated glial cells of human olfactory epithelium was cultured in Petri dishes and 12-well plates using a polylysine-laminin substrate. Primary cultures were subcultured after 10-15 days. The cell cultures were analyzed by phase contrast microscopy at all stages of culturing. A cytological study involved histological methods (trypan blue staining) and immunocytochemical visualization of GFAP, nestin, and low-affinity nerve growth factor receptors. At the final stage of culturing (5 passages) the monolayer cultures included 2 types of cells: GFAP- and p75-positive glial cells and nestin-positive fibroblasts.
Savchenko
E A
EA
Department of Biological Psychiatry, V. P. Serbskii State Research Center of Social and Forensic Medicine, Russia.
Andreeva
N A
NA
Dmitrieva
T B
TB
Viktorov
I V
IV
Chekhonin
V P
VP
D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology*; D009831:Olfactory Mucosa / Q000166:cytology*