title: Screening of grapes and wine for azoxystrobin, kresoxim-methyl and trifloxystrobin fungicides by HPLC with diode array detection.
abstract: The Quinone outside Inhibitors (QoI) are one of the most important and recent fungicide groups used in viticulture and also allowed by Integrated Pest Management. Azoxystrobin, kresoxim-methyl and trifloxystrobin are the main active ingredients for treating downy and powdery mildews that can be present in grapes and wines. In this paper, a method is reported for the analysis of these three QoI-fungicides in grapes and wine. After liquid-liquid extraction and a clean-up on commercial silica cartridges, analysis was by isocratic HPLC with diode array detection (DAD) with a run time of 13 min. Confirmation was by solid-phase micro-extraction (SPME), followed by GC/MS determination. The main validation parameters for the three compounds in grapes and wine were a limit of detection up to 0.073 mg kg(-1), a precision not exceeding 10.0% and an average recovery of 93% +/- 38.
authors: LEPAE-DEQ, Faculdade de Engenharia da Universidade do Porto, Portugal.
authors: Susana
authors: S
authors: De Melo Abreu
authors: Manuela
authors: M
authors: Correia
authors: Paulo
authors: P
authors: Herbert
authors: Lúcia
authors: L
authors: Santos
authors: Arminda
authors: A
authors: Alves
mesh_terms: D000085:Acetates / Q000032:analysis; D000179:Acrylates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis*; D007097:Imines / Q000032:analysis; D008689:Methacrylates; D010573:Pesticide Residues / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D015203:Reproducibility of Results; D000073739:Strobilurins; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000085:Acetates; D000179:Acrylates; D005659:Fungicides, Industrial; D007097:Imines; D008689:Methacrylates; D010573:Pesticide Residues; D010648:Phenylacetates; D011743:Pyrimidines; D000073739:Strobilurins; C469328:kresoxim-methyl; C467051:trifloxystrobin; C087670:azoxystrobin