[Antioxidative activity of water-soluble extracts from Fructus Polygoni Orientalis].
OBJECTIVE
To study the antioxidative activity of the water-soluble extracts from Fructus Polygoni Orientalis.


METHODS
Malondialdehyde and superoxide dismutase were measured by thiobarbituric acid assay, nitrite method, respectively. The activity of glutathione peroxidase was determined by 5,5'-dithionbis (2-nitrobenzoic acid) photometric method. Lipofuscin was determined by Sohal method in the brain of senile model mice induced by D-galactose. The extracts of 0.8, 1.6 and 3.2 g.kg(-1).d(-1) were administered to the mice separately through oral gavage daily for 7 weeks.


RESULTS
Malondialdehyde content increased, activities of superoxide dismutase and glutathione peroxidase in the heart, liver and kidney decreased. Levels of lipofuscin in the brain of senile model mice induced by D-galactose increased. The three doses of the extracts could inhibit the levels of lipofuscin in the brain, malondialdehyde in the heart, liver and kidney. These results showed that there was a negative dose-effect relationship. The extracts also up-regulated the levels of superoxide dismutase and glutathione peroxidase in the heart, liver and kidney with a positive dose-effect relationship.


CONCLUSIONS
The water-soluble extracts exert its antioxidative activity by eliminating free hydroxyl radicals, increasing activities of superoxide dismutase and glutathione peroxidase.
Lei
Xiao-yan
XY
Department of Pediatrics, People's Hospital of Gansu Province, Lanzhou 730000, China.
Xu
Ai-xia
AX
Gao
Xiang
X
Ge
Bin
B
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005638:Fruit / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D028502:Polygonum / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase