title: A new fabrication process for a microchip electrophoresis device integrated with a three-electrode electrochemical detector.
abstract: We report here a novel and simple process for the fabrication of a poly(methyl methacrylate) (PMMA)-based microchip electrophoresis device, integrated with a screen-printed three-electrode electrochemical detector that does not require a replicate mold. In this approach, a photoresist layer constitutes both an adhesion layer and side walls of 50 mum wide and 50 mum tall microfluidic channels on a screen-printed three-electrode PMMA substrate. Openings were drilled for buffer reservoirs on an additional piece of PMMA, then the final device was bonded in a PMMA/photoresist/PMMA sandwich configuration. This process is inexpensive, less time-consuming, and simpler compared with traditional fabrication methods. The combination of this PMMA-based microchip fabrication together with screen-printed electrode technology holds great promise for the mass production of a single-use micrototal analytical system. Successful determination of uric acid and L-ascorbic acid with the presented system validates its utility. In combination with a suitable electrochemical detector, this device holds much promise for the determination of other analytes in various biological samples for medical and clinical diagnosis.
authors: Department of Chemistry, National Chung Hsing University, Taichung, Taiwan.
authors: Dong-Mung
authors: DM
authors: Tsai
authors: Kuan-Wen
authors: KW
authors: Lin
authors: Jyh-Myng
authors: JM
authors: Zen
authors: Hung-Yu
authors: HY
authors: Chen
authors: Ray-Hua
authors: RH
authors: Hong
mesh_terms: D001205:Ascorbic Acid / Q000652:urine; D004553:Electric Conductivity; D004563:Electrochemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D006801:Humans; D008839:Microelectrodes; D008904:Miniaturization; D019904:Polymethyl Methacrylate / Q000737:chemistry; D014527:Uric Acid / Q000652:urine
chemical_list: D014527:Uric Acid; D019904:Polymethyl Methacrylate; D001205:Ascorbic Acid