SARS coronavirus detection methods.
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
Lau
Susanna K P
SK
Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.
Che
Xiao-Yan
XY
Woo
Patrick C Y
PC
Wong
Beatrice H L
BH
Cheng
Vincent C C
VC
Woo
Gibson K S
GK
Hung
Ivan F N
IF
Poon
Rosana W S
RW
Chan
Kwok-Hung
KH
Peiris
J S Malik
JS
Yuen
Kwok-Yung
KY
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D006801:Humans; D019590:Nucleocapsid Proteins / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000652:urine
D019590:Nucleocapsid Proteins