[Determination of perfluoropropane in canine whole blood by purge and trap concentrator-GC-MS].
OBJECTIVE
To develop a method for direct determination of perfluoropropane in canine whole blood and to study its pharmacokinetics after a suspension of perfluoropropane-containing albumin microcapsules was administered intravenously.


METHODS
Perfluoropropane-containing albumin microcapsule suspension was administered intravenously to anesthetized canines at the dosage of 0.6 mL x kg(-1). Whole blood samples were collected and added directly into the purging glass tube in Tekmar 3000 Purge and Trap Concentrator coupled with a GC-MS for the determination of perfluoropropane. The pharmacokinetic parameters of perfluoropropane were calculated by non-compartment model statistics.


RESULTS
The linear range was 0.0168-4.03 mg x L(-1). The main pharmacokinetic parameters of perfluoropropane was obtained as follows: mean residence time (MRT) was (63 +/- 5) s, T1/2 was (44 +/- 4) s, Tmax was 30 s, Cmax was (2.20 +/- 0.20) mg x L(-1), AUC0-infinity was (96 +/- 11) mg x s x L(-1).


CONCLUSIONS
The method is sensitive, specific and simple. It can be used to determine fluorocarbon contained in microcapsule ultrasound contrast agents for studying its pharmacokinetics.
Yang
Li
L
Department of Pharmacy, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China. nfyang@126.com
Chen
Zhi-liang
ZL
Wang
Xin-ya
XY
D000418:Albumins; D000818:Animals; D019540:Area Under Curve; D002214:Capsules; D004285:Dogs; D005466:Fluorocarbons / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007275:Injections, Intravenous
D000418:Albumins; D002214:Capsules; D005466:Fluorocarbons; C042852:perflutren