title: Flow injection spectrophotometric determination of ofloxacin in pharmaceuticals and urine.
abstract: A sensitive and fast flow-injection spectrophotometric method for the determination of ofloxacin is proposed. The method is based on the formation of a yellow complex between ofloxacin and Fe (III), in sulphuric medium. The influence of FI and chemical variables were studied. The calibration graph resulting from measuring the absorbance at 420 nm is linear over the range 5.0 x 10(-6)8.0 x 10(-4)M (1.8-289 mg l(-1)) with a detection limit of 2.0 x 10(-6)M (0.72 mg l(-1)). The method is applied to the routine analysis of ofloxacin in pharmaceuticals and human urine.
authors: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain.
authors: M
authors: M
authors: Soledad García
authors: M
authors: M
authors: Isabel Albero
authors: Concepción
authors: C
authors: Sánchez-Pedreño
authors: Mustafa
authors: M
authors: Salem Abuherba
mesh_terms: D002138:Calibration; D005290:Ferric Compounds / Q000737:chemistry; D017022:Flow Injection Analysis; D006801:Humans; D015242:Ofloxacin / Q000008:administration & dosage / Q000032:analysis / Q000652:urine*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013464:Sulfuric Acids / Q000737:chemistry; D013607:Tablets
chemical_list: D005290:Ferric Compounds; D004364:Pharmaceutical Preparations; D013464:Sulfuric Acids; D013607:Tablets; C024823:ferric sulfate; D015242:Ofloxacin; C033158:sulfuric acid