title: Mutation of retS, encoding a putative hybrid two-component regulatory protein in Pseudomonas aeruginosa, attenuates multiple virulence mechanisms.
abstract: Two-component regulatory systems play an important role in bacterial virulence. We report that mutation of a Pseudomonas aeruginosa gene designated retS (previously designated fimK; accession number PA4856) encoding a putative hybrid two-component regulator, attenuates multiple virulence mechanisms. The retS mutant was selected from a Tn5 transposon library of the cytotoxic P. aeruginosa strain PA103 based upon expression of a small-colony phenotype suggestive of reduced surface-associated "twitching" motility, a property dependent upon type IV pili. Subsequent analysis revealed that the mutant expressed pilin, albeit at lower levels than wild-type PA103. In a murine model of corneal infection, retS mutation was associated with delayed disease development and altered pathology. In vitro, retS mutants demonstrated loss of acute cytotoxic activity towards corneal epithelia as determined by trypan blue exclusion and by LDH release assays (P<0.0001). This coincided with loss of ExsA-regulated type III secretion. Mutation of retS also impaired ExsA-independent pathogenic mechanisms. When compared to the exsA mutant of PA103, retS mutants exhibited reduced epithelial adherence and invasion and reduced intracellular survival within the cells after invasion. Time-lapse video microscopy revealed that retS mutants, compared to exsA mutants, had a reduced capacity to access, and move along, the basal cell surfaces of corneal epithelial cell monolayers. Taken together, these data suggest that the protein encoded by retS regulates various properties of P. aeruginosa including both ExsA-dependent and ExsA-independent virulence mechanisms.
authors: School of Optometry, 688 Minor Hall, University of California, Berkeley, CA 94720-2020, USA.
authors: Irandokht
authors: I
authors: Zolfaghar
authors: Annette A
authors: AA
authors: Angus
authors: Pil J
authors: PJ
authors: Kang
authors: Aaron
authors: A
authors: To
authors: David J
authors: DJ
authors: Evans
authors: Suzanne M J
authors: SM
authors: Fleiszig
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D003603:Cytotoxins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000472:pathogenicity*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003603:Cytotoxins; D004268:DNA-Binding Proteins; C095092:ExsA protein, bacteria; C507835:RetS protein, Pseudomonas aeruginosa; D015534:Trans-Activators; D037521:Virulence Factors