Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts.
Beta-adrenergic receptor signaling, desensitization, and downregualtion are fundamental mechanisms that contribute to both normal and altered myocardial function. The development of pharmacological and biochemical assays has provided the ability to measure alterations both in adrenergic receptor density and the subsequent coupling of adrenergic receptors downstream effectors, namely, adenylyl cyclase. Furthermore, transthoracic echocardiography of the murine heart has provided insight into changes in cardiac physiology that accompany altered adrenergic receptor signaling. The protocols described within this chapter provide the means to quantify beta-adrenergic density, measure adenylyl cyclase activity, and evaluate cardiac hypertrophy to better understand the mechanisms responsible for cardiac hypertrophy and heart failure.
Wolf
Matthew J
MJ
Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Tachibana
Hideo
H
Rockman
Howard A
HA
D000262:Adenylyl Cyclases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism* / Q000503:physiopathology; D002462:Cell Membrane / Q000378:metabolism; D004452:Echocardiography; D006439:Hemodynamics; D008024:Ligands; D051379:Mice; D009206:Myocardium / Q000166:cytology; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000378:metabolism*; D015398:Signal Transduction
D008024:Ligands; D011943:Receptors, Adrenergic, beta; D000262:Adenylyl Cyclases