Stress response in Cu2+ and Cd2+ exposed oysters (Crassostrea gigas): an immunohistochemical approach.
Localization of heat shock proteins (HSPs) and metallothioneins (MTs) was investigated in a marine bivalve (Crassostrea gigas) by immunohistochemical methods. Differential protein expression was demonstrated in digestive gland, gonad and gills, using a polyclonal antibody against C. gigas proteins. Application of this technique showed the cellular and tissue immunolabelling specificity of the two proteins. HSPs and MTs were localized in the epithelium of the digestive gland and gills in contact with the palleal compartment. For the first time, localization of MTs was observed in mature gametes of bivalve molluscs. Our results establish a basis for the use of immmunodetection techniques to study the tissue-specific localization of stress proteins in marine bivalves exposed to metal stress.
Moraga
Dario
D
Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Nicolas Copernic, F-29280, Plouzané, France. Dario.Moraga@univ-brest.fr
Meistertzheim
Anne-Leïla
AL
Tanguy-Royer
Séverine
S
Boutet
Isabelle
I
Tanguy
Arnaud
A
Donval
Anne
A
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D008668:Metallothionein / Q000032:analysis; D010125:Ostreidae / Q000187:drug effects* / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D003300:Copper; D008668:Metallothionein