title: Differential expression of the chsE gene encoding a chitin synthase of Aspergillus nidulans in response to developmental status and growth conditions.
abstract: Expression of chsE encoding one of the five chitin synthases of Aspergillus nidulans was analyzed. Expression of chsE was moderate in conidiophores, but somewhat weaker in vegetative mycelia. During sexual development, chsE was expressed strongly in young cleistothecia and hülle cells, but little in mature sexual structures. Deletion of chsE caused a significant decrease in the chitin content of the cell wall during early sexual development. Expression of chsE was increased by substituting glucose with lactose or by addition of 0.6M KCl or NaCl, but affected little by substituting glucose with sodium acetate. Consequently, chsE was shown to have a mode of expression distinct from those of the other chitin synthase genes, chsA, chsB and chsC.
authors: Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Jeong Im
authors: JI
authors: Lee
authors: Yeong Man
authors: YM
authors: Yu
authors: Yoo Mi
authors: YM
authors: Rho
authors: Bum Chan
authors: BC
authors: Park
authors: Jeong Hwan
authors: JH
authors: Choi
authors: Hee-Moon
authors: HM
authors: Park
authors: Pil Jae
authors: PJ
authors: Maeng
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response; D009997:Osmotic Pressure
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002686:Chitin; D002687:Chitin Synthase