Identification of cellular membrane proteins interacting with hepatitis B surface antigen using yeast split-ubiquitin system.
Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These include a resident protein of endoplasmic reticulum (thioredoxin-related transmembrane protein 2), an adaptor protein involved in clathrin-mediated endocytosis and HIV-mediated downregulation of CD4, and a co-receptor of coxsakie B virus. The significance of our findings is suggested by the identification of cellular membrane proteins interacting with other virus proteins. Further functional analysis of these HBsAg- interacting cellular membrane proteins should shed new insights on their role in HBV morphogenesis.
Toh
Qi Chun
QC
Hepatitis Viruses and Liver Cancer Research Laboratory, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive 05N-10, Singapore 637551.
Tan
Tuan Lin
TL
Teo
Wei Qiang
WQ
Ho
Chin Yee
CY
Parida
Subhajeet
S
Chen
Wei Ning
WN