Identification and characterization of ankyrin repeat and SOCS box-containing gene ASB15 in silico.
The ASBs are a family of ankyrin-repeat proteins that contain a C-terminal SOCS box motif, which was first identified in the suppressor of cytokine signaling (SOCS) proteins. Here, we identified the ASB15 gene by using bio-informatics. Human ASB15 gene, consisting of 12 exons, was located within human genome sequences RP11-390E23 (AC006333.3). Complete coding sequence of human ASB15 cDNA was determined by FLJ43370 cDNA (AK125360), which was identified by using partial coding sequence of human ASB15 cDNA (AF403033) as a query. Human, chimpanzee, bovine, canine, and murine ASB15 showed 88-99% total-amino acid identity. N-terminal and central seven ankyrin repeats, and C-terminal SOCS box were identified within ASB15 protein.
Yoshida
Kenichi
K
Department of Life Sciences, Meiji University, 1-1-1 Higashimita, Kawasaki, Kanagawa 214-8571, Japan. yoshida@isc.meiji.ac.jp
D000595:Amino Acid Sequence; D017089:Ankyrin Repeat / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D020224:Expressed Sequence Tags; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C505604:ASB15 protein, human; D018076:DNA, Complementary; D011506:Proteins