Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes.
Adipose tissue contains a stroma that can be easily isolated. Thus, human adipose tissue presents an source of multipotent stromal cells. In order to determine the implication of hematopoietic markers in adipocyte biology, we have defined part of the phenotype of the human adipose tissue-derived stromal cells, and compared this to fully differentiated adipocytes. Flow cytometry demonstrates that the protein expression phenotype of both cell types are similar and includes the expression of CD10, CD13, CD34, CD36, CD55, CD59 and CD65. No significant difference between subcutaneous and omental adipose tissue could be demonstrated concerning the expression of these markers. However, the expression of CD34, CD36 and CD65 is cell-dependent. While the expression of CD36 and CD65 doubled between stromal cells and mature adipocytes, the expression of CD34 decreased, despite this protein being present on the mature adipocyte. As CD34 is described as a stem cell marker and it being unlikely to be expressed on differentiated cells, this result was confirmed by immunostaining and western blot. The clear function of this protein on the adipocyte membrane remains to be determined. The characterization of new proteins on mature adipocytes could have broad implications for the comprehension of the biology of this tissue.
Festy
Franck
F
Laboratoire de Biochimie et Génétique Moléculaire, EA-2526, Université de La Réunion, Faculté des Sciences, 15 Avenue René Cassin, 97415, Saint Denis, France.
Hoareau
Laurence
L
Bes-Houtmann
Sandrine
S
Péquin
Anne-Marie
AM
Gonthier
Marie-Paule
MP
Munstun
Ashik
A
Hoarau
Jean Jacques
JJ
Césari
Maya
M
Roche
Régis
R
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009852:Omentum / Q000166:cytology / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000378:metabolism
D015703:Antigens, CD; D015415:Biomarkers; D008565:Membrane Proteins