ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL.
In human cells, the ELL family of transcription factors includes at least three members, which are all capable of stimulating the overall rate of elongation by RNA polymerase II by suppressing transient pausing by the enzyme at many sites along DNA. In this report, we identify the ELL-associated factors (EAF)1 and EAF2 as strong positive regulators of ELL elongation activity. Our findings provide insights into the structure and function of ELL family transcription factors, and they bring to light direct roles for the EAF proteins in regulation of RNA polymerase II transcription.
Kong
Stephanie E
SE
Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Banks
Charles A S
CA
Shilatifard
Ali
A
Conaway
Joan Weliky
JW
Conaway
Ronald C
RC
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007313:Insecta; D009363:Neoplasm Proteins / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D035602:Transcriptional Elongation Factors
D004268:DNA-Binding Proteins; C431383:EAF1 protein, human; C472771:EAF2 protein, human; C090862:ELL protein, human; D009363:Neoplasm Proteins; D010445:Peptide Elongation Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; D004247:DNA