[Production of polyclonal antibody against HBD-2 by immunization with recombinant GST-HBD-2 fusion protein].
For the purpose of detecting the HBD-2 expression at protein level, the recombinant prokaryotic expression vector pGEX-1lambdaT-HBD-2 was constructed and the E. coli-based product of GST-HBD-2 fusion protein was prepared. When rabbit was immunized with the fusion protein, the anti-serum against HBD-2 was produced. After caprylic acid and ammonium sulfate precipitation, high titer of specific polyclonal antibody against HBD-2, which was detected by ELISA and Western blot, was obtained. This result suggests that recombinant peptide fusion protein could be used instead of the conjugate of peptide-albumin or peptide-thyroid globulin to produce antibody. The obtained antibodies could be used for revealing the tissue distribution of HBD-2 and the regulation of its gene expression.
Wang
Guoxing
G
Research Unit of Infection & Immunity, West China Medical Center, Sichuan University, Chengdu 610041, China.
Feng
Yun
Y
Wu
Qi
Q
Yan
Ronghua
R
Li
Xun
X
Wang
Boyao
B
Huang
Ning
N
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D007114:Immunization; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D023083:beta-Defensins / Q000276:immunology*
D000906:Antibodies; D011993:Recombinant Fusion Proteins; D023083:beta-Defensins