title: Analysis of mechanism for human gammadelta T cell recognition of nonpeptide antigens.
abstract: Whereas human gammadelta T cells respond to nonpeptide antigens like pyrophosphomonoesters and alkyl amines in the primary reactions, only pyrophosphomonoesters provoke proliferative responses in the secondary responses. To elucidate the differences in stimulatory activity between the two groups of nonpeptide antigens, we systematically analyzed time courses of gene expressions by microarray analyses. While 253 genes were induced by stimulation with 2-methyl-3-butenyl-1-pyrophosphate (2M3B1PP), only 35 genes were detected after stimulation with isobutyl amine. Then, gammadelta T cells expressed various cytokines like XCL1-2, CCL3-4, TNF-alpha, and IFN-gamma in response to 2M3B1PP in a time-dependent manner, while transient expressions were observed in IBA during the time period. The differences in such responsiveness are likely to originate from the activation state of NFAT, which is involved in the expression of transcription factors, EGR1-3 and NR4A1-2, and might play a crucial role in effector functions of gammadelta T cells.
authors: Genome Science Division, Research Center for Advanced Science and Technology, The University of Tokyo, Meguro-Ku, Tokyo 153-8904, Japan. yseizi91@yahoo.co.jp
authors: Seiji
authors: S
authors: Yamashita
authors: Yoshimasa
authors: Y
authors: Tanaka
authors: Shuichi
authors: S
authors: Tsutsumi
authors: Hiroyuki
authors: H
authors: Aburatani
authors: Nagahiro
authors: N
authors: Minato
authors: Sigeo
authors: S
authors: Ihara
mesh_terms: D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D016207:Cytokines; D010455:Peptides; D016692:Receptors, Antigen, T-Cell, gamma-delta