title: Actin-based motility of endosomes is linked to the polar tip growth of root hairs.
abstract: Plant tip growth has been recognized as an actin-based cellular process requiring targeted exocytosis and compensatory endocytosis to occur at the growth cone. However, the identity of subcellular compartments involved in polarized membrane trafficking pathways remains enigmatic in plants. Here we characterize endosomal compartments in tip-growing root hair cells. We demonstrate their presence at the growing tip and differential distribution upon cessation of tip growth. We also show that both the presence of endosomes as well as their rapid movements within the tip region depends on an intact actin cytoskeleton and involves actin polymerization. In conclusion, actin-propelled endosomal motility is tightly linked to the polar tip growth of root hairs.
authors: Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany.
authors: Boris
authors: B
authors: Voigt
authors: Antonius C J
authors: AC
authors: Timmers
authors: Jozef
authors: J
authors: Samaj
authors: Andrej
authors: A
authors: Hlavacka
authors: Takashi
authors: T
authors: Ueda
authors: Mary
authors: M
authors: Preuss
authors: Erik
authors: E
authors: Nielsen
authors: Jaideep
authors: J
authors: Mathur
authors: Neil
authors: N
authors: Emans
authors: Harald
authors: H
authors: Stenmark
authors: Akihiko
authors: A
authors: Nakano
authors: Frantisek
authors: F
authors: Baluska
authors: Diedrik
authors: D
authors: Menzel
mesh_terms: D000199:Actins / Q000235:genetics / Q000502:physiology*; D000730:Androstadienes / Q000494:pharmacology; D017360:Arabidopsis; D020126:Brefeldin A / Q000494:pharmacology; D047630:Depsipeptides / Q000494:pharmacology; D011992:Endosomes / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008164:Luminescent Proteins; D046913:Medicago truncatula; D018613:Microscopy, Confocal; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000077191:Wortmannin
chemical_list: D000199:Actins; D000730:Androstadienes; D047630:Depsipeptides; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C413663:fluorescent protein 583; C057531:jasplakinolide; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; D000077191:Wortmannin