[Inhibitory effect of endostatin mediated by lipofectin on transplanted ovarian cancer].
OBJECTIVE
To study the inhibitory effect of endostatin mediated by lipofectin on transplanted ovarian cancer in nude mice.


METHODS
Constructed recombinant vector pVAX1-sEn expressing human endostatin protein was transfected into ovarian cancer cell line 3AO by lipofectin. mRNA of endostatin was detected by RT-PCR. The expression of endostatin in supernatants was detected by enzyme-linked immunosorbent assay (ELISA). The inhibitory effect of pVAX1-sEn on endothelial cell line ECV-204 was detected by methyl thiazolyl tetrazolium (MTT). By use of lipofectin mediated pVAX1-sEn for intratumor injection, the inhibitory effect on growth of ovarian cancer was observed.


RESULTS
The result of RT-PCR showed there was a specific band at 610 bp. The expression quantity of endostatin in transfected cell supernatant was (201 +/- 8) ng/ml by ELISA. MTT showed pVAX1-sEn transfected cell supernatant could effectively inhibit the growth of ECV-204, the highest inhibitory ratio was 42%. The tumor volumes in pVAX1-sEn treatment group was (0.85 +/- 0.18) cm(3), significantly smaller than that in normal saline control group (1.90 +/- 0.28) cm(3) and pVAX1 control group (1.78 +/- 0.32) cm(3) (P < 0.05). HE stain in tumor tissue showed that there were obvious necrosis cells in the pVAX1-sEn treatment group, but there were flourishly growing tumor cells in pVAX1 and normal saline control groups.


CONCLUSIONS
pVAX1-sEn mediated by lipofectin can effectively inhibit the growth of ovarian cancer.
Wei
Zeng-tao
ZT
Department of Obstetrics and Gynecology, Medical College, Shandong University, Jinan 250012, China.
Wang
Xiao-yan
XY
Dong
Jian-chun
JC
Ma
Chun-hong
CH
Zhao
Dong
D
Zhang
Yan
Y
Sun
Wen-sheng
WS
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D043169:Endostatins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology*; D010714:Phosphatidylethanolamines; D011897:Random Allocation; D014162:Transfection
D043169:Endostatins; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine