Acute lung injury with endotoxin or NO2 does not enhance development of airway epithelium from bone marrow.
Adult marrow-derived stem cells can localize to lung and acquire immunophenotypic characteristics of lung epithelial cells. Lung injury increases recruitment of the marrow-derived cells. We speculated that comparing patterns of lung engraftment following different lung injuries would provide insight into potential mechanisms by which marrow-derived cells were recruited to lung. To evaluate this, adult female C57Bl/6 mice irradiated and engrafted with marrow from adult male transgenic GFP mice were exposed to either intranasal inhalation of endotoxin (25 microg/mouse) or 3 days of 25 ppm NO(2) and then compared 1 or 3 months later to transplanted but otherwise uninjured mice. In all cases, the majority of marrow-derived cells recruited to lung were CD45(+) leukocytes. In lungs of transplanted but otherwise uninjured mice, small numbers of CD45(-) donor-derived cells in alveolar septae stained positively for pro-surfactant protein C. Rare donor-derived cells located in the airway epithelium stained positively with cytokeratin. Subsequent exposure of engrafted mice to NO(2) or endotoxin did not significantly increase the number or pattern of donor-derived CD45(-) cells found in recipient lungs. These results suggest that NO(2) or endotoxin lung injury does not result in significant engraftment of marrow-derived cells in lung.
Beckett
Travis
T
Pulmonary and Critical Care, Vermont Lung Center, University of Vermont College of Medicine, Burlington, 05405, USA.
Loi
Roberto
R
Prenovitz
Robert
R
Poynter
Matthew
M
Goncz
Kaarin K
KK
Suratt
Benjamin T
BT
Weiss
Daniel J
DJ
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000502:physiology*; D004731:Endotoxins; D004847:Epithelial Cells; D005260:Female; D049452:Green Fluorescent Proteins; D007962:Leukocytes; D008168:Lung / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide; D011650:Pulmonary Alveoli / Q000528:radiation effects*; D011828:Radiation Chimera; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D014916:Whole-Body Irradiation
D004731:Endotoxins; D049452:Green Fluorescent Proteins; D009569:Nitric Oxide