New synchrotron radiation circular dichroism end-station on DISCO beamline at SOLEIL synchrotron for biomolecular analysis.
The novel Synchrotron Radiation Circular Dichroism (SRCD) technique is becoming a new tool of investigation for the molecular structures of biomolecules, like proteins, carbohydrates or others bio-materials. Here, we describe the characteristics of a new experimental end-station for circular dichroism studies, in construction on DISCO beamline at SOLEIL synchrotron (Saint-Aubin, France). This experimental end-station will be an open facility for the community of researchers in structural biology. In order to show the kind of information accessible with this type of technique, we give an example: the conformational study of the galactose mutarotase from Escherichia coli, an enzyme involved in the galactose metabolism. This study was made using an operational SRCD station available at SRS (Daresbury Laboratory, UK).
Miron
Simona
S
Centre de Biophysique Moléculaire, UPR 4301 CNRS, Université d'Orléans, Rue Charles Sadron, 45071 Orléans cedex 02, France. miron@cnrs-orleans.fr
Réfregiers
Matthieu
M
Gilles
Anne-Marie
AM
Maurizot
Jean-Claude
JC
D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism / Q000295:instrumentation* / Q000639:trends; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017356:Synchrotrons / Q000295:instrumentation*