Proliferative and re-defferentiative effects of photo-immobilized micro-patterned hyaluronan surfaces on chondrocyte cells.
A photo-immobilisation procedure was utilised to create two different micro-patterned surfaces (tracks 25 and 5 microm wide) of hyaluronan (Hyal) on polyethylene-terephthalate (PET) previously plasma activated. Aim of the study was to investigate the proliferation and re-differentiation capacity of articular chondrocytes cultured on micro-patterned Hyal, compared to homogeneous Hyal and plain plasma-treated (pt-)PET substrates. Cytotoxicity, cell proliferation, activation and differentiation of articular knee cartilage chondrocytes (Mongrel sheep) were evaluated after 14 days of culture. It was found that micro-patterned Hyal surfaces induced the adhesion, migration and alignment of chondrocytes, as shown by light and scanning electron microscopy. Furthermore, the same surfaces induced chondrocyte differentiation, with a significant increase of aggrecan and collagen type II production, while homogeneous Hyal and pt-PET surfaces did not.
Barbucci
Rolando
R
C.R.I.S.M.A. and Department of Chemical and Biosystems Sciences and Technologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy. barbucci@unisi.it
Torricelli
Paola
P
Fini
Milena
M
Pasqui
Daniela
D
Favia
Pietro
P
Sardella
Eloisa
E
d'Agostino
Riccardo
R
Giardino
Roberto
R
D053674:Aggrecans; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000473:pathology; D002358:Cartilage, Articular / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000737:chemistry; D003470:Culture Media / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D037181:Lectins, C-Type / Q000737:chemistry; D008027:Light; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry; D012756:Sheep; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013997:Time Factors; D014466:Ultraviolet Rays; D014945:Wound Healing
D053674:Aggrecans; D001672:Biocompatible Materials; D024043:Collagen Type II; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011093:Polyethylene Terephthalates; D011509:Proteoglycans; D006820:Hyaluronic Acid