Alterations in neutrophil (PMN) free intracellular alpha-keto acid profiles and immune functions induced by L-alanyl-L-glutamine, arginine or taurine.
The objective of this study was to determine the dose as well as duration of exposure-dependent effects of L-alanyl-L-glutamine, arginine or taurine on polymorphonuclear neutrophil (PMN) free alpha-keto acid profiles and, in a parallel study, on PMN immune functions. Exogenous L-alanyl-L-glutamine significantly increased PMN alpha-ketoglutarate, pyruvate PMN superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase (MPO) activity. Arginine also led to significant increases in alpha-ketoglutarate, pyruvate, MPO release and H2O2 generation. Formation of O2- on the other hand was decreased by arginine. Incubation with taurine resulted in lower intracellular pyruvate and alpha-ketobutyrate levels, decreased O2- and H2O2 formation and a concomitant significantly increased MPO activity. We therefore believe that considerable changes in PMN free-alpha-keto-acid profiles, induced for example by L-alanyl-L-glutamine, arginine or taurine, may be one of the determinants in cell nutrition that considerably modulates the immunological competence of PMN.
Mühling
J
J
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany. joerg.muehling@chiru.med.uni-giessen.de
Nickolaus
K A
KA
Halabi
M
M
Fuchs
M
M
Krüll
M
M
Engel
J
J
Wolff
M
M
Matejec
R
R
Langefeld
T W
TW
Welters
I D
ID
Menges
T
T
Dehne
M G
MG
Sablotzki
A
A
Hempelmann
G
G
D000328:Adult; D001120:Arginine / Q000494:pharmacology*; D004151:Dipeptides / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007651:Keto Acids / Q000737:chemistry / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000187:drug effects; D013654:Taurine / Q000494:pharmacology*; D013997:Time Factors
D004151:Dipeptides; D007651:Keto Acids; D013654:Taurine; D001120:Arginine; D009195:Peroxidase; C054122:alanylglutamine