Evaluation of Group B Streptococcus Differential Agar for detection and isolation of Streptococcus agalactiae.
In total, 320 vaginal or rectal swabs were cultured on Granada medium (GM) or Group B Streptococcus Differential Agar (GBSDA), and were also inoculated into LIM broth (Todd-Hewitt broth supplemented with selective antibiotics), for detection of group B Streptococcus (GBS). Overall, GBS isolates were detected on 53 of the 320 swabs; 47 of these isolates grew on both GM and GBSDA, five only on GBSDA, and one only following subculture from LIM broth. GBSDA appears to be a valid alternative to GM for the growth of GBS isolates from pregnant women.
Bou
G
G
Servicio de Microbiologia, Unidad de Investigacion, Complejo Hospitalario Universitario Juan Canalejo, La Coruña, Spain. germanbou@canalejo.org
Figueira
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M
Canle
D
D
Cartelle
M
M
Eiros
J M
JM
Villanueva
R
R
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D005260:Female; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D012007:Rectum / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods; D013290:Streptococcal Infections / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology
D003470:Culture Media; D000362:Agar