Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis.
Repetitive sequences in DNA molecules, some of which are palindromic, tend to form stable cruciforms. These are frequently located in promoter regions of a specific operon and origin of replication. Temperature gradient gel electrophoresis can be used to distinguish among various supercoiled DNA topoisomers and to ascertain whether or not the cruciform motif has been extruded. In the current study, this technique is implemented for the first time to address the role of temperature in cruciform extrusion from plasmids.
Víglasky
Viktor
V
Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safárik University, 04154 Kosice, Slovakia. vviglasky@yahoo.com
Danko
Patrik
P
Adamcík
Jozef
J
Valle
Francesco
F
Dietler
Giovanni
G
D004269:DNA, Bacterial / Q000737:chemistry; D045566:DNA, Cruciform / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods; D009690:Nucleic Acid Conformation; D010957:Plasmids; D013696:Temperature
D004269:DNA, Bacterial; D045566:DNA, Cruciform