Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas.
A Gram-negative, rod-shaped bacterium (designated strain UST030701-295(T)) with fast gliding motility was isolated from the surface of the sponge Lissodendoryx isodictyalis in the Bahamas. Colonies of UST030701-295(T) were yellow in colour, 2-4 mm in diameter, convex with a smooth surface and entire margins. UST030701-295(T) was heterotrophic, strictly aerobic and required NaCl for growth (1.0-4.0%). Growth was observed at pH 6.0-10.0 and at 12-44 degrees C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST030701-295(T) within the genus Winogradskyella of the family Flavobacteriaceae, sharing 94.7-95.8% similarity with the three recognized members of the genus. The G+C content of the DNA was 32.8 mol% and the predominant fatty acids were iso-C(15:1), iso-C(15:0), iso-C(15:0) 2-OH, iso-C(15:0) 3-OH, iso-C(16:0) 3-OH, C(16:1)omega7 and iso-C(17:0) 3-OH (together representing 75.4% of the total); these data supported the affiliation of UST030701-295(T) to the genus Winogradskyella. UST030701-295(T) differed from the three recognized species of Winogradskyella in 7-17 traits. Molecular evidence together with phenotypic characteristics suggests that UST030701-295(T) represents a novel species within the genus Winogradskyella, for which the name Winogradskyella poriferorum sp. nov. is proposed. The type strain is UST030701-295(T) (=NRRL B-41101(T)=JCM 12885(T)).
Lau
Stanley C K
SCK
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Tsoi
Mandy M Y
MMY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Li
Xiancui
X
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Plakhotnikova
Ioulia
I
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Dobretsov
Sergey
S
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Lau
Ken W K
KWK
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Wu
Madeline
M
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Wong
Po-Keung
PK
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, People's Republic of China.
Pawlik
Joseph R
JR
Center for Marine Science, University of North Carolina at Wilmington, USA.
Qian
Pei-Yuan
PY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
D000818:Animals; D015373:Bacterial Typing Techniques; D001444:Bahamas; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S