title: Production of sordarin and related metabolites by the coprophilous fungus Podospora pleiospora in submerged culture and in its natural substrate.
abstract: Rabbit pellets collected from the field were colonized by Podospora pleiospora at the exclusion of other coprophilous fungi, suggesting antibiosis. In liquid culture, P. pleiospora produced sordarin (1); sordarin B (2), a new compound in which sordarose is replaced by rhamnose; hydroxysordarin (3); and sordaricin (4). The major compounds 1 and 2 exhibited minimum inhibitory concentrations of 0.5-2.5 microg ml(-1) against the yeasts Nematospora coryli and Sporobolomyces roseus, but showed little or no activity against bacteria or coprophilous filamentous fungi. In liquid culture, the production of 1 and 2 together amounted to 2.7 microg ml(-1), whereas in rabbit dung only 1 was produced at a similar concentration (2.3 microg g(-1) fresh weight). The biosynthesis of these substances was unaffected by the presence of inoculum of other fungi tested (Sporobolomyces roseus or Penicillium claviforme) in liquid culture or on dung. Sordarin-type natural products are therefore synthesized by P. pleiospora at sufficiently high concentrations to account for antibiosis against yeasts, but not against filamentous fungi.
authors: Department of Biotechnology, University of Kaiserslautern, Paul-Ehrlich-Str. 23, D-67663 Kaiserslautern, Germany. rwsweber@rhrk.uni-kl.de
authors: Roland W S
authors: RW
authors: Weber
authors: Anja
authors: A
authors: Meffert
authors: Heidrun
authors: H
authors: Anke
authors: Olov
authors: O
authors: Sterner
mesh_terms: D000818:Animals; D000898:Antibiosis; D017077:Culture Media, Conditioned; D004224:Diterpenes / Q000378:metabolism / Q000494:pharmacology; D007192:Indenes / Q000378:metabolism* / Q000494:pharmacology; D008372:Manure / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D048468:Podospora / Q000378:metabolism / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D012210:Rhamnose; D012988:Soil Microbiology; D013237:Stereoisomerism; D015003:Yeasts / Q000187:drug effects / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D004224:Diterpenes; D007192:Indenes; D008372:Manure; D011500:Protein Synthesis Inhibitors; C458899:hydroxysordarin; C116705:sordaricin; C003329:sordarin; D012210:Rhamnose