Identification of the apoptosis inhibitor gene p49 of Spodoptera litura multicapsid nucleopolyhedrovirus.
Baculoviruses possess two types of genes that suppressed apoptosis, p35 and inhibitor of apoptosis (iap). Computer-assisted analysis indicated that Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF55 (designated as the p49 gene) display 79 and 31% amino acid identity with Spodoptera littoralis (Spli)MNPV P49 and Autographa californica (Ac)MNPV P35, respectively, Splt MNPV putative P49 contains a peptide cleavage site TVTDG recognized by death caspases. In marker rescue assay, Splt-p49 was able to suppress apoptosis induced by infection of a mutant AcMNPV deficient in p35 and rescue the mutant virus replication from apoptosis in Sf-9 cells.
Yu
Mei
M
State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275 Guangzhou, PR China.
Li
Zhaofei
Z
Yang
Kai
K
Lin
Tiehao
T
Gong
Yingxue
Y
Pan
Lijing
L
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D051034:Inhibitor of Apoptosis Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D018411:Spodoptera / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D051034:Inhibitor of Apoptosis Proteins; C117171:P49 protein, Spodoptera littoralis nucleopolyhedrovirus; D014764:Viral Proteins