Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypes.
HAT-B is a yeast histone acetyltransferase composed of Hat1, Hat2 and Hif1 proteins. We demonstrate that a hat2 mutant or a hat1hat2 double mutant, but not a hat1 mutant, have an extended life-span. Transcriptome analysis shows that the single hat mutants are not very different from wild type. However, the comparison of the hat1 and hat2 transcriptomes shows that they are different. The hat1hat2 double mutant shows a transcriptional phenotype similar to that of the hat1 mutant but strongly enhanced. These results indicate that Hat2p could have additional functions in the cell to those of Hat1p.
Rosaleny
Lorena E
LE
Departament de Bioquímica i Biologia Molecular, Universitat de València, Dr. Moliner 50, E46100 Burjassot, Spain.
Antúnez
Oreto
O
Ruiz-García
Ana B
AB
Pérez-Ortín
José E
JE
Tordera
Vicente
V
D000123:Acetyltransferases / Q000235:genetics*; D017353:Gene Deletion; D051548:Histone Acetyltransferases; D047468:Immunoprecipitation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D037761:Sirtuins / Q000378:metabolism; D016615:Telomere; D014158:Transcription, Genetic
D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; C110880:histone acetyltransferase type B complex; D037761:Sirtuins