The effect of hyperbaric oxygen treatment on the renal functions in septic rats: relation to oxidative damage.
OBJECTIVE
To investigate the effects of hyperbaric oxygen (HBO) treatment on renal functions and damage in septic rats.


METHODS
The animals were divided into four groups, each containing ten animals: control, hyperbaric oxygen, sepsis, and sepsis/hyperbaric oxygen. One milliliter of saline containing live Escherichia coli cells (2.1 x 10(9)) was injected intraperitoneally to induce sepsis. The groups treated with HBO were given five sessions of 2 atmospheres absolute of 100% oxygen at intervals of 6 h. Blood, urine, and tissue samples were then collected, and the functional renal parameters, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and catalase activities were examined.


RESULTS
The reduced glomerular filtration rate and urine flow returned to normal levels after HBO treatment; however, the increase in fractionated sodium excretion continued. The increased MDA levels in the renal cortex and medulla also decreased to the level of the control group. In the sepsis group, both the SOD and catalase activities decreased in the renal cortex, while a reduction was observed only in the catalase activity in the medulla. The reduced enzyme activities significantly increased in the sepsis/hyperbaric oxygen group.


CONCLUSIONS
HBO treatment has a beneficial effect on renal dysfunction in sepsis. The probable reason for this effect is the reduction in oxidative damage because of the increase in antioxidative capacity.
Edremitlioğlu
Mustafa
M
Department of Physiology, Kirikkale University School of Medicine, 71100 Kirikkale, Turkey.
Kiliç
Dilek
D
Oter
Sükrü
S
Kisa
Uçler
U
Korkmaz
Ahmet
A
Coşkun
Omer
O
Bedir
Orhan
O
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D007668:Kidney / Q000503:physiopathology; D007674:Kidney Diseases / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications*
D000975:Antioxidants