title: Detection of human immunodeficiency virus type 1 RNA by in situ hybridization in oral mucosa epithelial cells from anti-HIV-1 positive patients.
abstract: Several in vitro studies have shown that HIV-1 can infect CD4 negative epithelial cells of different origin including normal human oral keratinocytes, but whether this infection of mucosal epithelial cells occurs in vivo is still unclear. In this report, the presence and cell types infected by HIV-1 in paraffin embedded oral mucosa biopsies from 17 anti-HIV-1 positive patients have been examined by in situ hybridization and immunohistochemistry. As controls, oral mucosa biopsies from eight patients without HIV-1 infection markers were also analyzed. The results showed that 8 out of the 17 anti-HIV-1 positive patients had HIV-1 RNA detectable in plasma. Positive hybridization signals were observed in the mucosa biopsies from 14 of the 17 anti-HIV-1 patients (82.3%). The mean percentage of cells showing HIV-1 RNA was 2.64% +/- 1.77% (range: 1% to 5.5%). No differences in the mean percentage of HIV-1 infected cells were found between patients with and without HIV-1 RNA in plasma (3.01% +/- 1.57% vs. 3.4% +/- 1.27% respectively), or between untreated patients and patients under antiretroviral therapy (2.83% +/- 1.63% vs. 3.42% +/- 1.29% respectively). Immunohistochemical detection of S-100 antigen, cytokeratin and CD4 showed that hybridization signals appeared in cytokeratin positive cells and CD4 positive cells but not in S-100 positive cells. In conclusion, this study has demonstrated that HIV-1 infects and replicates in oral mucosa epithelial cells in vivo and that these cells could represent a reservoir of the virus that may escape to the currently used antiretroviral therapy.
authors: Fundacióon para el estudio de las Hepatitis Virales, Madrid, Spain.
authors: Elena
authors: E
authors: Rodríguez-Iñigo
authors: Esther
authors: E
authors: Jiménez
authors: Javier
authors: J
authors: Bartolomé
authors: Nuria
authors: N
authors: Ortiz-Movilla
authors: Begoña
authors: B
authors: Bartolomé Villar
authors: Juan
authors: J
authors: José Arrieta
authors: Felix
authors: F
authors: Manzarbeitia
authors: Vicente
authors: V
authors: Carreño
mesh_terms: D000328:Adult; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D009061:Mouth Mucosa / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis*; D019562:Viral Load
chemical_list: D012367:RNA, Viral