title: Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells.
abstract: Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.
authors: The International Livestock Research Institute (ILRI), Nairobi, Kenya.
authors: B
authors: B
authors: Lambson
authors: V
authors: V
authors: Nene
authors: M
authors: M
authors: Obura
authors: T
authors: T
authors: Shah
authors: P
authors: P
authors: Pandit
authors: O
authors: O
authors: Ole-Moiyoi
authors: K
authors: K
authors: Delroux
authors: S
authors: S
authors: Welburn
authors: R
authors: R
authors: Skilton
authors: E
authors: E
authors: de Villiers
authors: R
authors: R
authors: Bishop
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D026863:Ixodidae / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D019476:Insect Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides