The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.
El-Sayed
Najib M
NM
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA. nelsayed@tigr.org
Myler
Peter J
PJ
Bartholomeu
Daniella C
DC
Nilsson
Daniel
D
Aggarwal
Gautam
G
Tran
Anh-Nhi
AN
Ghedin
Elodie
E
Worthey
Elizabeth A
EA
Delcher
Arthur L
AL
Blandin
Gaëlle
G
Westenberger
Scott J
SJ
Caler
Elisabet
E
Cerqueira
Gustavo C
GC
Branche
Carole
C
Haas
Brian
B
Anupama
Atashi
A
Arner
Erik
E
Aslund
Lena
L
Attipoe
Philip
P
Bontempi
Esteban
E
Bringaud
Frédéric
F
Burton
Peter
P
Cadag
Eithon
E
Campbell
David A
DA
Carrington
Mark
M
Crabtree
Jonathan
J
Darban
Hamid
H
da Silveira
Jose Franco
JF
de Jong
Pieter
P
Edwards
Kimberly
K
Englund
Paul T
PT
Fazelina
Gholam
G
Feldblyum
Tamara
T
Ferella
Marcela
M
Frasch
Alberto Carlos
AC
Gull
Keith
K
Horn
David
D
Hou
Lihua
L
Huang
Yiting
Y
Kindlund
Ellen
E
Klingbeil
Michele
M
Kluge
Sindy
S
Koo
Hean
H
Lacerda
Daniela
D
Levin
Mariano J
MJ
Lorenzi
Hernan
H
Louie
Tin
T
Machado
Carlos Renato
CR
McCulloch
Richard
R
McKenna
Alan
A
Mizuno
Yumi
Y
Mottram
Jeremy C
JC
Nelson
Siri
S
Ochaya
Stephen
S
Osoegawa
Kazutoyo
K
Pai
Grace
G
Parsons
Marilyn
M
Pentony
Martin
M
Pettersson
Ulf
U
Pop
Mihai
M
Ramirez
Jose Luis
JL
Rinta
Joel
J
Robertson
Laura
L
Salzberg
Steven L
SL
Sanchez
Daniel O
DO
Seyler
Amber
A
Sharma
Reuben
R
Shetty
Jyoti
J
Simpson
Anjana J
AJ
Sisk
Ellen
E
Tammi
Martti T
MT
Tarleton
Rick
R
Teixeira
Santuza
S
Van Aken
Susan
S
Vogt
Christy
C
Ward
Pauline N
PN
Wickstead
Bill
B
Wortman
Jennifer
J
White
Owen
O
Fraser
Claire M
CM
Stuart
Kenneth D
KD
Andersson
Björn
B
D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy / Q000469:parasitology; D004260:DNA Repair; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D006801:Humans; D008540:Meiosis; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D005810:Multigene Family; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D016615:Telomere / Q000235:genetics; D014344:Trypanocidal Agents / Q000494:pharmacology / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; D018626:Retroelements; D014344:Trypanocidal Agents