Osteoblast growth promotion by protein electrostatic self-assembly on biodegradable poly(lactide).
Extracellular matrix (ECM)-like coating was developed on biodegradable biomaterials based on the electrostatic self-assembly (ESA) technique to promote osteoblast growth. Poly(ethylenimine) (PEI) was first employed to obtain a stable positively charged surface on poly (DL-lactide) (PDL-LA) substrate. Gelatin was selected as ECM-like biomacromolecule to deposit on the activated PDL-LA substrate using the ESA technique. zeta-Potential results showed alternating charge of polyelectrolytes (PEI/gelatin) layering on PDL-LA microspheres. Quartz crystal microbalance (QCM) measurement further verified the gradual deposition of PEI/gelatin on PDL-LA thin film. Osteoblast cells (MC3T3) were chosen to test the cell behavior on modified PDL-LA substrates. The osteoblast test about cell activity, intracellular total DNA content, total protein content and cell morphology by SEM investigation on ECM-like multilayer-modified PDL-LA substrate showed to promote osteoblast growth. Comparing conventional coating methods, polyelectrolyte multilayers are easy and stable to prepare. It may be a good choice for the surface modification of complex biomedical devices. These very flexible systems allow broad medical applications for drug delivery and tissue engineering.
Zhu
Huiguang
H
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Ji
Jian
J
Shen
Jiacong
J
D016475:3T3 Cells; D000818:Animals; D001673:Biodegradation, Environmental; D001861:Bone Regeneration; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005109:Extracellular Matrix; D005780:Gelatin / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology* / Q000627:therapeutic use; D011506:Proteins; D055672:Static Electricity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011506:Proteins; C033616:poly(lactide); D005780:Gelatin