title: [Reproducibility of exhaled nitric oxide (FENO) measurements in healthy subjects].
abstract: The aim of the study was to evaluate the short-term variability of FENO in healthy subjects.
  
  
  METHODS
  33 healthy volunteers (26 F, 7 M) aged 32.6 +/- 9.5 yrs with body mass index (BMI) of 23.3 +/- 3 kg/m2 participated in the study. Exhaled nitric oxide was analyzed on 5 consecutive days with a chemiluminescence analyzer (NIOX, Aerocrine, Sweden) according to the ATS recommendations. The exhalation flow was between 0.045 and 0.055 l/s. The measurements were performed at the same time of the day and the subjects were asked to refrain from eating and drinking for at least one hour before the analysis.
  
  
  RESULTS
  The mean value of FENO for the whole group was 13.9 +/- 5.4 ppb, there were no correlations between FENO and age, BMI, sex or the concentration of ambient nitric oxide. Day-to-day coefficient of variation was 13.3 +/- 5.3% (range 4.6 - 23.9%), the value of pooled SD - 2.1 ppb and ICC (intraclass correlation coefficient) was 0.84. No relationship was observed between variability of FENO and intervals between measurement of exhaled nitric oxide and intake of food or beverages.
  
  
  CONCLUSIONS
  Chemiluminescence analysis of FENO with NIOX is a highly reproducible method, however one has to take into account the possibility of about 13% variability of FENO within 5 days.
authors: Katedra i Klinika Chorób Wewnetrznych, Pneumonologii i Alergologii A. M. w Warszawie.
authors: Marta
authors: M
authors: Kumor
authors: Tadeusz
authors: T
authors: Przybyłowski
authors: Marta
authors: M
authors: Maskey-Warzechowska
authors: Katarzyna
authors: K
authors: Hildebrand
authors: Adam
authors: A
authors: Fangrat
authors: Piotr
authors: P
authors: Bielicki
authors: Katarzyna
authors: K
authors: Górska
authors: Justyna
authors: J
authors: Kościuch
authors: Joanna
authors: J
authors: Kucińska
authors: Ryszarda
authors: R
authors: Chazan
mesh_terms: D000328:Adult; D015992:Body Mass Index; D045853:Exhalation; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009569:Nitric Oxide