Effect of cationic surfactants on the interaction between sodium perfluorooctanoate and beta-lactoglobulin.
The interaction between the fluorocarbon surfactant, sodium perfluorooctanoate (SPFO), and beta-lactoglobulin (BLG) was studied. In particular, the effects of cationic surfactants, such as alkyltriethylammonium bromide (C(n)NE, n=8, 10, 12), on SPFO-BLG interaction were examined. It was shown that the anionic fluorocarbon surfactant, SPFO, was a strong denaturant of BLG. The ability of SPFO to denature BLG could be weakened by the addition of C(n)NE. The effect of C(n)NE on SPFO-BLG interaction was related to the hydrocarbon chain length of C(n)NE, and also the molar ratio of the added C(n)NE to the SPFO in SPFO-BLG solutions ([C(n)NE]/[SPFO]). Our findings might provide a way to design surfactant systems that are less denaturing to proteins or tailor the ability of surfactant to denature proteins through the appropriate mixing with other surfactants.
Lu
Run-Chao
RC
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Cao
Ao-Neng
AN
Lai
Lu-Hua
LH
Xiao
Jin-Xin
JX
D002210:Caprylates / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002942:Circular Dichroism; D004553:Electric Conductivity; D005466:Fluorocarbons / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
D002210:Caprylates; D002412:Cations; D005466:Fluorocarbons; D007782:Lactoglobulins; D013501:Surface-Active Agents; C023036:perfluorooctanoic acid