title: In vitro echogenicity characterization of poly[lactide-coglycolide] (plga) microparticles and preliminary in vivo ultrasound enhancement study for ultrasound contrast agent application.
abstract: OBJECTIVE
  This work includes (1) the characterization of a reproducible poly[lactide-coglycolide] (PLGA) microparticle preparation with an optimial mean diameter and size distribution and (2) the preliminary in vivo ultrasonographic investigation of PLGA microparticles.
  
  
  METHODS
  A first series of PLGA microparticle preparations (1 to 15 mum) was acoustically characterized on a hydrodynamic device to select the most appropriate for ultrasound contrast agent application. Preparations of 3-microm microparticles were selected, characterized at different doses, and then injected into 20 melanoma grafted mice for contrast-enhanced power Doppler ultrasonography evaluation.
  
  
  RESULTS
  The 3-microm microparticles (3.26-microm mean diameter with 0.41-microm standard deviation) led to in vitro enhancement of 18.3 dB at 0.62 mg/mL. In vivo experiments showed 47% enhancement of intratumoral vascularization detection after PLGA injection, significantly correlated (P < 0.0001) with preinjection intravascularization and tumoral volume. No toxicity was histologically observed.
  
  
  CONCLUSIONS
  The 3-microm PLGA microparticles provided significant enhancement in vitro and in vivo without any toxicity.
authors: Département d'Imagerie et Laboratoire d'Imagerie du Petit Animal Laboratoire, Institut Gustave Roussy, Villejuif, France.
authors: Sonia
authors: S
authors: Lavisse
authors: Angelo
authors: A
authors: Paci
authors: Valerie
authors: V
authors: Rouffiac
authors: Cecile
authors: C
authors: Adotevi
authors: Paule
authors: P
authors: Opolon
authors: Pierre
authors: P
authors: Peronneau
authors: Philippe
authors: P
authors: Bourget
authors: Alain
authors: A
authors: Roche
authors: Michel
authors: M
authors: Perricaudet
authors: Elias
authors: E
authors: Fattal
authors: Nathalie
authors: N
authors: Lassau
mesh_terms: D000818:Animals; D003287:Contrast Media; D066298:In Vitro Techniques; D019344:Lactic Acid; D008545:Melanoma / Q000000981:diagnostic imaging*; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011134:Polysaccharides; D015203:Reproducibility of Results; D018608:Ultrasonography, Doppler
chemical_list: D003287:Contrast Media; D011108:Polymers; D011134:Polysaccharides; C059891:SHU 508; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid