[Experimental study on the effects of interferon-gamma gene on Tenon's capsule fibroblasts in vitro].
OBJECTIVE
To investigate the effects of interferon-gamma (IFN-gamma) gene on Tenon's capsule fibroblasts in vitro.


METHODS
Using lipofectAMINE, IFN-gamma gene was transferred to human Tenon's capsule fibroblasts with plasmid pcDNA3IFN-gamma in vitro. Cells treated with plasmid pcDNA3 without IFN-gamma gene were used as the controls. The transfected fibroblasts were screened by G418. The expression of IFN-gamma was determined by RT-PCR, immunohistochemistry and flow cytometry assay. The effects of IFN-gamma on the proliferation of Tenon's capsule fibroblasts were evaluated by flow cytometry and MTT.


RESULTS
The Tenon's capsule fibroblasts transferred the IFN-gamma gene could express the IFN-gamma RNA and protein transiently. The proliferation of the fibroblasts transferred the IFN-gamma gene was inhibited.


CONCLUSIONS
The proliferation of the Tenon's capsule fibroblasts in vitro can be inhibited by transferred IFN-gamma gene.
Lan
Yu-qing
YQ
Department of Ophthalmology, the Second affiliated Hospital, Zhongshan University, Guangzhou 510120, China.
Ge
Jian
J
Lin
Ming-kai
MK
Zheng
Jian-liang
JL
Wei
Jing
J
Liu
Hai-quan
HQ
Li
Yan-yan
YY
D003228:Conjunctiva / Q000187:drug effects*; D003238:Connective Tissue / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000235:genetics*; D011994:Recombinant Proteins; D014162:Transfection
D011994:Recombinant Proteins; D007371:Interferon-gamma