[Conditions for SARS-CoV cultivation and inactivation].
OBJECTIVE
To study the method for cultivation and inactivation of SARS-CoV.


METHODS
In order to choose the sensitive cell strain and the best infection dose of the virus, Vero, Vero-E6 and 2BS cell lines were infected with SARS-CoV. The cultivation temperature was selected among 25 degrees C, 33 degrees C and 37 degrees C. The best inactivating time and effect were observed with beta-propiolactone whose concentration ranged from 1:2000 to 1:20,000 at room temperature.


RESULTS
Vero and Vero-E6 cell lines were sensitive to SARS-CoV. The cytopathic changes of the cells were 75% at 37 degrees C in 5 percent CO2 incubator after infection. SARS-CoV was inactivated completely in beta-propiolactone (1:4000). The toxicity of beta-propiolactone was hydrolyzed completely when the inactivated virus was cultured for 16 hours at 2 degrees C, 8 degrees C and in water bath for 2 hours at 37 degrees C.


CONCLUSIONS
The titer of SARS-CoV was the highest when it was cultured in Vero or Vero-E6 cells for 72 hours at 37 degrees C in 5 percent CO2 incubator. SARS-CoV was inactivated completely in beta-propiolactone when its concentration was 1:4000 and the interaction time was 1 hour.
Zhang
Song-le
SL
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net
Ma
Li-juan
LJ
Tian
Guang
G
Zhang
Liang-yan
LY
Zhang
Xue-ying
XY
Wang
Xi-liang
XL
D000818:Animals; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D011420:Propiolactone / Q000494:pharmacology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors; D014709:Vero Cells; D038301:Virus Inactivation / Q000187:drug effects*
D011420:Propiolactone