[Synthesis of daidzein and genistein by streptomycetes and their effect on production of antibiotics].
The strain Streptomyces globisporus 1912 and its derivative mutants 3-1 high and deficient producers of landomycin E and B1, accordingly, formed, when grown on the minimum and soy media, isoflavones which after the extraction and purification by thin layer chromatography were identified as daidzein and genistein on the basis of Rf indices and maxima of absorption in UV light identical to standard isoflavones. Mutant, B2, defective in biosynthesis of antibiotic, did not produce the above isoflavones and restored the synthesis of landomycin E under action of genistein introduced to the medium or secreted by the B1 culture in the cosynthesis test. Daidzein did not restore biosynthesis of antibiotic by this mutant. The cosynthesis test was used for preliminary selection of Streptomyces strains producing genistein. It was shown that 10% of 200 fresh isolated soil Spreptomycetes produced daidzein and genistein. The synthesis of isoflavones by streptomycetes can take place de novo on the minimum medium or by means of beta-galactosidase hydrolysis of the corresponding soy glycosides on soy medium. Genistein increased the production of polyketide antibiotics landomycines A and E, tetracenomycin and chlortetracycline by 11-42% and repressed the synthesis of urdamycin by 15%.
Matseliukh
B P
BP
Polishchuk
L V
LV
Lutchenko
V V
VV
Bambura
O I
OI
Kopiyko
O P
OP
D000617:Aminoglycosides / Q000096:biosynthesis / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000145:classification / Q000494:pharmacology*; D003470:Culture Media; D019833:Genistein / Q000302:isolation & purification / Q000378:metabolism*; D007529:Isoflavones / Q000096:biosynthesis* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009154:Mutation; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D007529:Isoflavones; C113593:landomycin E; C004742:daidzein; D019833:Genistein