Visualization of sperm by immunohistochemistry: application of alternative counterstains.
The aim of this study was to evaluate the application of hematoxylin, safranin, light green and picric acid as counterstain in sperm immunohistochemistry. This is important to visualize the best staining procedure and to determine the advantage of picric acid as a counterstain in some situations. Picric acid used for counterstaining in the immunohistochemical procedure gives the best image of reaction on sperms when DAB was used as a chromogen.
Erdogan
D
D
Department of Histology and Embryology, Gazi Hospital, Gazi University, Faculty of Medicine, Besevler, Ankara, Turkey. denizer@gazi.edu.tr
Elmas
C
C
Ilgaz
C
C
D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007248:Infertility, Male / Q000175:diagnosis; D008297:Male; D008853:Microscopy / Q000379:methods; D010851:Picrates; D013094:Spermatozoa / Q000166:cytology* / Q000473:pathology; D013194:Staining and Labeling
D010851:Picrates; C005858:picric acid