Magnetic force-based mesenchymal stem cell expansion using antibody-conjugated magnetoliposomes.
Recently, there has been an accumulation of evidence indicating that human mesenchymal stem cells (MSCs, multipotent cells resident in the bone marrow) are useful for autologous cell transplantation. However, only small numbers of MSCs have been obtained in bone marrow aspirates. We have developed a novel methodology for enriching and proliferating MSCs from bone marrow aspirates using antibody-conjugated magnetoliposomes (AMLs). The AMLs are liposomes conjugated to anti-CD105 antibody (immunoliposomes) and contain magnetite nanoparticles (diameter 10 nm). In the present study, the AMLs were added to a small volume (1 mL) of human bone marrow aspirate. After a 1-h incubation period, the bone marrow aspirates containing AMLs were seeded into 10-cm tissue culture dishes, and a disk-shaped magnet (diameter 2.2 cm; height 1 cm; 4000 Gauss) was positioned under the dish to enrich MSCs by magnetic force. The MSCs proliferated, forming colonies at the site where the magnet was positioned. In contrast, no colonies and very few viable cells were observed in ordinary culture based on plastic-adherent tendencies of cells without use of AMLs. These results suggest that this AML culture method can rapidly and efficiently expand a small number of MSCs into numbers suitable for clinical application.
Ito
Akira
A
Department of Biotechnology, School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
Hibino
Eri
E
Shimizu
Kazunori
K
Kobayashi
Takeshi
T
Yamada
Yoichi
Y
Hibi
Hideharu
H
Ueda
Minoru
M
Honda
Hiroyuki
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015703:Antigens, CD / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D000071063:Endoglin; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D007074:Immunoglobulin G; D008081:Liposomes; D011956:Receptors, Cell Surface