The impact of pronuclear orientation to select chromosomally normal embryos.
OBJECTIVE
To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied.


METHODS
Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclear-nucleoli morphology.


RESULTS
Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-good-morphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology.


CONCLUSIONS
Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.
Borges
Edson Júnior
EJ
Fertility-Centro de Fertilização Assistida, São Paulo, Brazil.
Rossi
Lia Mara
LM
Farah
Leila
L
Guilherme
Patricia
P
Rocha
Claudia C
CC
Ortiz
Valdemar
V
Iaconelli
Assumpto Júnior
AJ
D002455:Cell Division; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005306:Fertilization; D006801:Humans; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*