title: A variety of synergistic and antagonistic interactions mediated by cis-acting DNA motifs regulate gene expression in plant cells and modulate stability of the transcription complex formed on a basal promoter.
abstract: Several synthetic promoters containing a variety of commonly found cis-acting DNA sequence motifs were constructed to study the motif-motif and motif-protein interactions involved in gene expression in plants. Transient expression of the reporter gene gusA in tobacco leaves was used to demonstrate that several sequence elements can be arranged upstream of a basal promoter to function synergistically in enhancing gene expression. A cis-acting DNA motif could function as an activator by itself as well as a synergizing activator in the presence of other homologous as well as heterologous motifs in the neighbourhood. The function of a complex promoter comprising several activation motifs was arrested nearly completely in vivo, following titration with any one of the motifs. The results suggested a hierarchical assembly of several motif-binding factors, leading to the stabilization of the transcriptional complex formed on the TATA-box.
authors: National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India.
authors: Samir V
authors: SV
authors: Sawant
authors: Kanti
authors: K
authors: Kiran
authors: Rajesh
authors: R
authors: Mehrotra
authors: Chandra Prakash
authors: CP
authors: Chaturvedi
authors: Suraiya A
authors: SA
authors: Ansari
authors: Pratibha
authors: P
authors: Singh
authors: Niraj
authors: N
authors: Lodhi
authors: Rakesh
authors: R
authors: Tuli
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins