Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.
The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.
Sato
Masahiro
M
The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masasato@is.icc.u-tokai.ac.jp
Ohtsuka
Masato
M
Ohmi
Yuhsuke
Y
D017103:Bacillus Phages / Q000235:genetics*; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D004996:Ethidium / Q000494:pharmacology; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D012984:Software; D013698:Templates, Genetic; D013997:Time Factors; D014466:Ultraviolet Rays
D004270:DNA, Circular; D049452:Green Fluorescent Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004996:Ethidium