title: Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
abstract: The Arabidopsis gene COI1 is required for jasmonic acid (JA)-induced growth inhibition, resistance to insect herbivory, and resistance to pathogens. In addition, COI1 is also required for transcription of several genes induced by wounding or by JA. Here, we use microarray gene transcription profiling of wild type and coi1 mutant plants to examine the extent of the requirement of COI1 for JA-induced and wound-induced gene transcription. We show that COI1 is required for expression of approximately 84% of 212 genes induced by JA, and for expression of approximately 44% of 153 genes induced by wounding. Surprisingly, COI1 was also required for repression of 53% of 104 genes whose expression was suppressed by JA, and for repression of approximately 46% of 83 genes whose expression was suppressed by wounding. These results indicate that COI1 plays a pivotal role in wound- and JA signalling.
authors: School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK.
authors: Alessandra
authors: A
authors: Devoto
authors: Christine
authors: C
authors: Ellis
authors: Andreas
authors: A
authors: Magusin
authors: Hur-Song
authors: HS
authors: Chang
authors: Charles
authors: C
authors: Chilcott
authors: Tong
authors: T
authors: Zhu
authors: John G
authors: JG
authors: Turner
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000872:Anthocyanins / Q000096:biosynthesis; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D016000:Cluster Analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013314:Stress, Mechanical; D013729:Terpenes / Q000378:metabolism
chemical_list: D000085:Acetates; D000872:Anthocyanins; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013729:Terpenes; C072239:methyl jasmonate