Vaccine efficacy of recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis.
Thirty-seven kilodalton glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Edwardsiella tarda was suggested to be an effective vaccine candidate against E. tarda infection in previous research. For developing a vaccine, obtaining GAPDH in large quantities is necessary. In this study, we determined the complete nucleotide sequence of the gene that encodes GAPDH of E. tarda, and overexpressed the GAPDH of E. tarda by using the Escherichia coli expression system. We immunized Japanese flounder with recombinant GAPDH (rGAPDH) and evaluated its vaccine efficacy. Our results showed that rGAPDH effectively protected Japanese flounder from experimental E. tarda infection, and will contribute to the development of a vaccine against E. tarda.
Liu
Ying
Y
Fish Disease Laboratory, Department of Aquaculture, Faculty of Agriculture, Kochi, Japan.
Oshima
Syun-ichirou
S
Kurohara
Kenrou
K
Ohnishi
Kouhei
K
Kawai
Kenji
K
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D020609:Edwardsiella tarda / Q000737:chemistry* / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000517:prevention & control* / Q000662:veterinary; D004925:Escherichia / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)