A modified cell culture method for autologous chondrocyte transplantation.
Autologous chondrocyte transplantation (ACT) is a promising method to treat chondral and osteochondral defects. This study introduced a modified method for cell culture in ACT. Porcine chondrocytes were cultured for 3 weeks under low hydrostatic pressure at 250 Pa. The results showed that the dry weight of the cartilage-like membrane in the loading group was 3.0 times more than the control group (no loading) (p < 0.01), and cell numbers were significantly increased by 3.1 times (p < 0.01) after a 3-week culture. Compared with the fresh tissue sample, the mRNA expression of collagen II was not statistically different and the mRNA of aggrecan was only slightly decreased by 19%. These data suggest that the hydrostatic pressure at this level significantly increased the cell numbers and biosynthesis of cultured chondrocytes.
Lee
Chun-Yi
CY
Department of Orthopaedic Surgery, China Medical University Hospital, Taichung, Taiwan.
Liu
Xuhui
X
Hsu
Horng-Chaung
HC
Wang
Der-Yean
DY
Luo
Zong-Ping
ZP
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D019902:Chondrocytes / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D024043:Collagen Type II / Q000096:biosynthesis; D006874:Hydrostatic Pressure; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D014182:Transplantation, Autologous
D024043:Collagen Type II; D012333:RNA, Messenger