A prospective randomized trial of N-acetyl cysteine administration during cold preservation of the donor liver for transplantation.
OBJECTIVE
N-acetyl cysteine (NAC), an anti oxidant and a glutathione precursor, is effective in ameliorating liver injury of Tylenol overdose. There is experimental evidence that it also reduces ischemia reperfusion (I/R) injury. This clinical study was undertaken to study the effect of NAC administered in the donor operation.


METHODS
22 patients were randomized to receive NAC (IV & Portal flush) or no NAC (Control Group) during donor operation. Peak AST levels and 1-hour post-reperfusion biopsies were used to assess I/R injury. Episodes of acute rejection were recorded together with immunosuppressive drug levels.


RESULTS
There were 4 exclusions (re-exploration for post-operative hemorrhage x3, OLT for acute liver failure x1). The two groups (n = 9 each) were matched for recipient and donor ages and sex. Viral hepatitis accounted for cirrhosis in 3 patients in NAC Group and 6 patients in Control Group. Statistically, Cold and warm ischemia times were not significantly different as was the use of blood and blood products in both groups. Serum peak AST levels were similar and post- reperfusion biopsy showed moderate to severe reperfusion injury in 3 recipients in the NAC Group and 4 in the Control Group. Excluding ones associated with low Tacrolimus levels (n = 4), there were 6 episodes of acute rejection (2- mild, 4- moderate) in the NAC Group and 5 in the Control Group (3- mild,1- moderate, 1- severe).


CONCLUSIONS
In this pilot study, NAC administered during donor operation did not show a protective effect on I/R injury or on acute cellular rejection.
Khan
Abdaal W
AW
Hepato-Pancreato-Biliary and Transplantation Unit, Royal Free and UCL Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF.
Fuller
Barry J
BJ
Shah
Sudeep R
SR
Davidson
Brian R
BR
Rolles
Keith
K
D000111:Acetylcysteine / Q000627:therapeutic use*; D000328:Adult; D000368:Aged; D003080:Cold Temperature; D005260:Female; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D010865:Pilot Projects; D011446:Prospective Studies; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D020858:Tissue and Organ Harvesting / Q000009:adverse effects*
D000111:Acetylcysteine