title: Molecular cloning of enantioselective ester hydrolase from Bacillus pumilus DBRL-191.
abstract: A gene from Bacillus pumilus expressed under its native promoter was cloned in Escherichia coli. Recombinant B. pumilus esterase (BPE) affects the kinetic resolution of racemic mixtures such as unsubstituted and substituted 1-(phenyl)ethanols (E approximately 33-103), ethyl 3-hydroxy-3-phenylpropanoate (E approximately 45-71), trans-4-fluorophenyl-3-hydroxymethyl-N-methylpiperidine (E approximately 10-13) and ethyl 2-hydroxy-4-phenylbutyrate (E approximately 7). The enzyme is composed of a 34-amino acid signal peptide and a 181-amino acid mature protein corresponding to a molecular weight of approximately 19.2kD and pI approximately 9.4. 3-D the structural model of the enzyme built by homology modelling using the atomic coordinates from the crystal structure of B. subtilis lipase (LipA) showed a compact minimal alpha/beta hydrolase fold.
authors: Biotechnology Division, Regional Research Laboratory, Canal Road, Jammu Tawi 180001, India.
authors: Shafaq
authors: S
authors: Rasool
authors: Sarojini
authors: S
authors: Johri
authors: Syed
authors: S
authors: Riyaz-ul-Hassan
authors: Qurrat-ul-Ain
authors: QU
authors: Maqbool
authors: Vijeshwar
authors: V
authors: Verma
authors: Surrinder
authors: S
authors: Koul
authors: Subhash C
authors: SC
authors: Taneja
authors: Ghulam N
authors: GN
authors: Qazi
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004950:Esterases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004952:Esters / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D004952:Esters; D004950:Esterases