title: Determination of the relative amounts of Gag and Pol proteins in foamy virus particles.
abstract: We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68Gag moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71Gag plus p68Gag:p85RT),12:1 (p68Gag:p85RT), and 10:1 (pr71Gag plus p68Gag:p40IN). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.
authors: Institut für Virologie, Medizinische Fakultät, Technische, Universität Dresden, Germany. Marc.Cartellieri@mailbox.tu-dresden.de
authors: Marc
authors: M
authors: Cartellieri
authors: Wolfram
authors: W
authors: Rudolph
authors: Ottmar
authors: O
authors: Herchenröder
authors: Dirk
authors: D
authors: Lindemann
authors: Axel
authors: A
authors: Rethwilm
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D002213:Capsid / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism*; D015684:Gene Products, pol / Q000378:metabolism*; D015751:Genes, pol; D011994:Recombinant Proteins / Q000378:metabolism; D016092:Spumavirus / Q000378:metabolism* / Q000472:pathogenicity; D014771:Virion / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D015683:Gene Products, gag; D015684:Gene Products, pol; D011994:Recombinant Proteins