Expression of a calcineurin gene improves salt stress tolerance in transgenic rice.
Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase and has multiple functions in animal cells including regulating ionic homeostasis. We generated transgenic rice plants that not only expressed a truncated form of the catalytic subunit of mouse calcineurin, but also were able to grow and fertilize normally in the field. Notably, the expression of the mouse calcineurin gene in rice resulted in its higher salt stress tolerance than the non-transgenic rice. Physiological studies have indicated that the root growth of transgenic plants was less inhibited than the shoot growth, and that less Na+ was accumulated in the roots of transgenic plants after a prolonged period of salt stress. These findings imply that the heterologous calcineurin plays a significant role in maintaining ionic homeostasis and the integrity of plant roots when exposed to salt. In addition, the calcineurin gene expression in the stems of transgenic plants correlated with the increased expression of the Rab16A gene that encodes a group 2-type late-embryogenesis-abundant (LEA) protein. Altogether our findings provide the first genetic and physiological evidence that expression of the mouse calcineurin protein functionally improves the salt stress tolerance of rice partly by limiting Na+ accumulation in the roots.
Ma
Xujun
X
Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Qian
Qian
Q
Zhu
Dahai
D
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D019703:Calcineurin / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C102591:Rab16A protein, Oryza sativa; D002734:Chlorophyll; D008353:Mannitol; D012965:Sodium Chloride; D012964:Sodium; D019703:Calcineurin; D011188:Potassium