Synthesis of magnetic chelator for high-capacity immobilized metal affinity adsorption of protein by cerium initiated graft polymerization.
A novel magnetic chelator with high adsorption capacity of protein by immobilized metal affinity adsorption was prepared by cerium (IV) initiated graft polymerization of tentacle-type polymer chains with iminodiacetic acid (IDA) chelating group on magnetic particles with hydroxyl groups. The micron-sized magnetic poly(vinyl acetate-divinylbenzene) (PVAc-DVB) particles were prepared by a modified suspension polymerization in the presence of oleic acid-coated magnetite nanoparticles and subsequently modified by ester exchange reaction to introduce functional hydroxyl groups. Bovine hemoglobin (BHb) was selected as a model protein to investigate the adsorption capacity of these magnetic particles. The magnetic particles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), superconducting quantum interference device (SQUID) magnetometry, diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), and X-ray diffraction (XRD). The results showed that the magnetic particles had an average size of 5 microm and superparamagnetism with saturation magnetization of 20.0 emu/g at room temperature. The protein adsorption indicated that the graft polymerization of tentacle type polymer chains on the magnetic particles could produce magnetic adsorbents with high adsorption capacity (1428.21 mg/g) and low nonspecific adsorption of protein. The magnetic particles with grafted tentacle polymer chains have potential application in large-scale affinity separation of proteins.
Ma
Z Y
ZY
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, P. R. China.
Guan
Y P
YP
Liu
X Q
XQ
Liu
H Z
HZ
D000327:Adsorption; D002563:Cerium / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis*; D005583:Fourier Analysis; D008280:Magnetics; D008670:Metals / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019301:Oleic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D014961:X-Ray Diffraction
D002614:Chelating Agents; D008670:Metals; D011108:Polymers; D011506:Proteins; D019301:Oleic Acid; D002563:Cerium