Effect of glutathione on UV induction of prophage lambda.
The effect of glutathione (GSH) on the ultraviolet (UV) induction of lambda prophage was investigated in lysogenic Escherichia coli. The data showed that extracellular GSH could inhibit the UV induction of lambda prophage. The inhibitory rates were concentration dependent, and the maximal rate obtained was 94% with 3.0 M GSH. The effect was also measured in three different lambda lysogens: a wild-type strain (wt), an isogenic GSH-deficient strain, and an isogenic strain producing increased amounts of GSH. The result showed that when subjected to UV irradiation (254 nm, 60 J m(-2)), GSH-deficient strain was approximately fivefold more sensitive to be lysed than wt, whereas the strain with higher intracellular GSH levels was only 28% susceptible to be lysed. With electron spin resonance and spin trapping techniques, we observed that free radical signals occurred in the suspensions of UV irradiated lysogenic cells and the intensity of signals was influenced by GSH levels. These results indicate that GSH can significantly inhibit the UV induction of lambda prophage, and that this effect is correlated to its capacity to scavenge free radicals generated after UV irradiation.
Liu
Yancheng
Y
College of Life Sciences, Wuhan University, Wuhan, 430072 People's Republic of China.
Zhang
Qiuju
Q
Fang
Chengxiang
C
Zhu
Shiqiao
S
Tang
Yali
Y
Huang
Shaoyi
S
D010582:Bacteriophage lambda / Q000235:genetics / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000821:virology*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology*; D008242:Lysogeny / Q000187:drug effects; D039002:Prophages / Q000528:radiation effects*; D014466:Ultraviolet Rays
D005609:Free Radicals; D005978:Glutathione