[Cbfal's effect on chondrogenic gene expression in fibroblast].
OBJECTIVE
Core-binding factor a1, Cbfa1, which belongs to the runt-domain gene family, is an essential transcription factor for osteoblastic differentiation and osteogenesis. To examine the effects of Cbfa1 exhibit on gene expression which involving in the chondrogenesis.


METHODS
On the first, using RT-PCR method, we directly cloned Cbfa1/Runx2 full length cDNA from E13.5 days mouse embryos limb buds. Consequently Cbfa1/Runx2 was cloned into pcDNA3.1 plasmid to make a eukaryotic expression vector. All the clones were proved to be correct by enzyme cutting and sequencing analysis. On the second, we used pcDNA3.1-Cbfa1 to tansfect fibroblast. 48 hours later, we contrasted the gene transcription of Sox9, Sox5 and Sox6 between tansfected and untransfected. 72 hours later, type II collagen have also been examined by Western blotting.


RESULTS
Cbfa1 overexpression in fibroblast can upregulate the expression of Sox9 and type II collagen, but it have no effect on the expression of Sox5 and Sox6.


CONCLUSIONS
Cbfa1 may involve in the regulation of chondrogenesis.
Zhang
Wei
W
Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhu
Ya-ping
YP
Wang
Jun
J
Deng
Lian-fu
LF
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D024043:Collagen Type II / Q000096:biosynthesis / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006609:High Mobility Group Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D055758:SOX9 Transcription Factor; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D024043:Collagen Type II; D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse; D014157:Transcription Factors