[Effects of ethyl pyruvate on splenocyte proliferation and apoptosis in burn rats with delayed resuscitation].
OBJECTIVE
To investigate the effects of ethyl pyruvate (EP) on splenocyte proliferation and apoptosis in burn rats with delayed resuscitation, and its potential underlying mechanism.


METHODS
Seventy two male Wistar rats were randomly divided into sham-scalded control group (n=24), scald group (n=24), and scald with EP treatment group (n=24). Animals were sacrificed on days 1, 3, and 5 postburn, and spleen samples were collected to determine splenocyte proliferation and apoptosis.


RESULTS
Splenic lymphocyte proliferation response to T cell mitogen, concanavalin A (Con A), as significantly depressed from 1 to 5 days after burn injury (all P<0.05). Meanwhile, burn injury resulted in a marked increase in splenic CD3(+)CD4(+)T lymphocyte apoptosis in comparison with that in sham-scalded controls on day 1 postburn (P<0.05). Treatment with EP after burns resulted in a dramatic restoration of lymphocyte proliferation response and reduction of splenic CD3(+)CD4(+)T lymphocyte apoptosis compared with scald group (P<0.05).


CONCLUSIONS
Administration of EP can markedly improve the splenocyte proliferation response and inhibit splenic CD3(+)CD4(+)T lymphocyte apoptosis in thermally injured rats.
Dong
Ning
N
Burns Institute, 304th Hospital Affiliated to General Hospital of PLA, Beijing 100037, China.
Yao
Yong-ming
YM
Dong
Yue-qing
YQ
Liu
Hui
H
Wei
Peng
P
Yu
Yan
Y
Sheng
Zhi-yong
ZY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002056:Burns / Q000276:immunology* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D011773:Pyruvates / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000473:pathology
D011773:Pyruvates; C046522:ethyl pyruvate