Lidoflazine combined with nucleotide precursors increases ATP content and adenosine production in cardiomyocytes.
We have previously identified that the nucleoside transport blocker dipyridamole increases adenosine production but may cause depletion of the nucleotide pool in cardiomyocytes during extended exposure and that this effect was abolished by co-administration of adenine and ribose. The present study aimed to establish whether lidoflazine, a newer generation of nucleoside transport inhibitor with calcium antagonist properties, would cause a similar effect. We conclude that lidoflazine did not affect the nucleotide pool while the combined application of lidoflazine with precursors of nucleotide resynthesis increased ATP concentration and further enhanced adenosine production.
Kalsi
K K
KK
Heart Science Centre, Imperial College at Harefield Hospital, Harefield, Middlesex UB96JH, UK. K.Kalsi@imperial.ac.uk
Smolenski
R T
RT
Yacoub
M H
MH
D000225:Adenine / Q000494:pharmacology; D000241:Adenosine / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D008013:Lidoflazine / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry; D051381:Rats; D012266:Ribose / Q000494:pharmacology; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
D009711:Nucleotides; D014665:Vasodilator Agents; D012266:Ribose; D000255:Adenosine Triphosphate; D008013:Lidoflazine; D000225:Adenine; D000241:Adenosine