title: Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.
abstract: The loss of E-cadherin expression and the translocation of beta-catenin to the nucleus are frequently associated with the metastatic conversion of epithelial cells. In the nucleus, beta-catenin binds to the TCF/LEF-1 (T-cell factor/ lymphoid enhancer factor) transcription factor family resulting in the activation of several genes, some of them having important implications in tumour progression. In our study, we investigated the potential regulation of monocyte chemotactic protein-1 (MCP-1/CCL2) expression by the beta-catenin/TCF pathway. This CC-chemokine has been implicated in tumour progression events such as angiogenesis or tumour associated macrophage (TAM) infiltration. We thus demonstrated that MCP-1 expression correlates with the reorganization of the E-cadherin/beta-catenin complexes. Indeed, MCP-1 was expressed by invasive breast cancer cells (MDA-MB-231, BT549 and Hs578T), which do not express E-cadherin but was not produced by noninvasive breast cancer cell lines (MCF7 and T47D) expressing high level of E-cadherin. In addition, the MCP-1 promoter was activated in BT549 breast cancer cells transfected with beta-catenin and TCF-4 cDNAs. The MCP-1 mRNA level was similarly upregulated. Moreover, we showed that MCP-1 mRNA was downregulated after transfection with a siRNA against beta-catenin in both BT549 and Hs578T cells. Our results therefore identify MCP-1 as a target of the beta-catenin/TCF/LEF pathway in breast tumour cells, a regulation which could play a key role in breast tumour progression.
authors: Laboratory of Developmental and Tumour Biology, University of Liège, Liège, Belgium.
authors: Melanie
authors: M
authors: Mestdagt
authors: Myriam
authors: M
authors: Polette
authors: Giovanna
authors: G
authors: Buttice
authors: Agnes
authors: A
authors: Noël
authors: Atsuhisa
authors: A
authors: Ueda
authors: Jean-Michel
authors: JM
authors: Foidart
authors: Christine
authors: C
authors: Gilles
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D015820:Cadherins / Q000096:biosynthesis*; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D018450:Disease Progression; D015536:Down-Regulation; D006801:Humans; D009389:Neovascularization, Pathologic; D011401:Promoter Regions, Genetic; D051820:TCF Transcription Factors / Q000502:physiology*; D058118:Transcription Factor 7-Like 2 Protein; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D051176:beta Catenin / Q000502:physiology*
chemical_list: C111835:CCL2 protein, human; D015820:Cadherins; D018932:Chemokine CCL2; D051820:TCF Transcription Factors; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D051176:beta Catenin