alpha(v)beta(3) Integrin in central nervous system tumors.
alpha(v)beta(3) Is an integrin specifically expressed in endothelial cells of newly forming blood vessels. Integrin-mediated angiogenesis is hypothesized to play a central role in the development and the progression of central nervous system neoplasms. Accordingly, it is considered a potential target for antiangiogenic therapy. In the current study, we compare the expression of alpha(v)beta(3) in ependymomas, oligodendrogliomas, pilocytic astrocytomas, medulloblastomas, and vestibular schwannomas (acoustic neuromas). Samples of 5 tumors of each of the 5 tumor types were harvested surgically and frozen. After the pathological diagnosis was confirmed, immunohistochemistry was performed using an anti- alpha(v)beta(3) monoclonal antibody (LM609). The expression of alpha(v)beta(3) was assessed using a 4-tiered (0-3) grading scheme reflecting the percentage of positively staining vessels. All vestibular schwannomas demonstrated strong (grade 3) alpha(v)beta(3) expression. The expression was uniformly prominent in Antoni B regions of the tumors. Of 5 ependymomas, 4 demonstrated uniformly strong alpha(v)beta(3). Oligodendrogliomas, medulloblastomas, and pilocytic astrocytomas demonstrated more variable alpha(v)beta(3). alpha(v)beta(3) may contribute significantly to angiogenesis in vestibular schwannomas and ependymomas. Despite the high vascular density of oligodendrogliomas, pilocytic astrocytomas, and medulloblastomas, these tumors had variable moderate alpha(v)beta(3) expression. This discrepancy suggests temporal and/or regional variability in the angiogenesis in these types of tumor. This study provides the first demonstration of alpha(v)beta(3) expression in vestibular schwannomas, medulloblastomas, and pilocytic astrocytomas.
Lim
Michael
M
Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA. mklim@stanford.edu
Guccione
Samira
S
Haddix
Terri
T
Sims
Leroy
L
Cheshier
Samuel
S
Chu
Pauline
P
Vogel
Hannes
H
Harsh
Griffith
G
D001254:Astrocytoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000098:blood supply* / Q000378:metabolism*; D004806:Ependymoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D039482:Integrin alpha5 / Q000096:biosynthesis*; D008527:Medulloblastoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D009464:Neuroma, Acoustic / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009837:Oligodendroglioma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology
D039482:Integrin alpha5