title: [Study on the presence of hepatitis B virus in first-trimester villi in pregnant women with hepatitis B surface antigen positive].
abstract: OBJECTIVE
  To observe the presence of hepatitis B virus (HBV) in first-trimester villi cells from pregnant women carrying HBsAg.
  
  
  METHODS
  Immunohistochemical streptavidin-biotin peroxidase complex (SABC) staining with monoclonal HBsAg, hepatitis B core antigen (HBcAg) and PCR, in situ hybridization were used for detection of HBV infection markers in villi. Positive villi ultramicrostructures were observed with transmission electron microscope.
  
  
  RESULTS
  HBV was detected in 8 of 25 villi of HBsAg positive pregnant women, the positive rate was 32%. HBsAg was located in the decidual cell, trophoblastic cell and villous mesenchymal cell. HBV analog was detected in rough endoplasmic reticulum of trophoblastic cell.
  
  
  CONCLUSIONS
  HBV may infect villous cells in first-trimester pregnancy. It would be impossible for HBV to transmit the desmosomes.
authors: Department of Epidemiology, Fourth Military Medical University, Xi'an 710032, China.
authors: Wen-hui
authors: WH
authors: Chang
authors: De-zhong
authors: DZ
authors: Xu
authors: Yong-ping
authors: YP
authors: Yan
authors: Ke-jun
authors: KJ
authors: Du
authors: Ke
authors: K
authors: Men
authors: Jing-xia
authors: JX
authors: Zhang
authors: Jing-jun
authors: JJ
authors: Wang
authors: Jian-qiu
authors: JQ
authors: Xu
authors: En-di
authors: ED
authors: Zhang
authors: Chen
authors: C
authors: Liu
authors: Feng-mei
authors: FM
authors: Sun
mesh_terms: D002824:Chorionic Villi / Q000648:ultrastructure / Q000821:virology*; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000097:blood / Q000276:immunology / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011261:Pregnancy Trimester, First
chemical_list: D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens