[Comparison of the detection of anaerobic bacteria in haemoculture systems BACTEC Lytic/10 Anaerobic/F, BacT/Alert FAN Anaerobic and BacT/Alert FN Anaerobic].
The purpose of this study is to deal with the problem of anaerobic cultivation of clinical specimens and consider the possibility of using semi-automated blood culture instruments. The Bactec Lytic, BacT/Alert FAN Anaerobic and BacT/Alert FN Anaerobic bottles were inoculated with Bacteriodes fragilis, Clostridium perfringens, Peptostreptococcus anaerobius and Feingoldia magna strains. The times to detection (TTD) for positive bottles were evaluated with reference to the number of inoculated bacteria. Inoculation with the same suspension of equal bacterial strain resulted in shorter TTD values for all the Bactec Lytic bottles as compared with the BacT/Alert FAN Anaerobic or BacT/Alert FN Anaerobic bottles, respectively. Statistically significant differences were recorded (p=0,05) by Bacteroides fragilis and Clostridium perfringens species. Feingoldia magna was submitted to culture in the Bactec Lytic alone. The anaerobic blood culture bottles are deemed acceptable for application in the culture of anaerobc isolates drawn from other samples except for blood (namely when prompt delivery of the sample to the laboratory is impossible and the sample can suffer damage), however, only a limited range of microorganisms can be taken into account for the detection purposes. The Bactec Lytic bottles are more appropriately designed for the detection of anaerobic bacterial species compared to the BacT/Alert FAN Anaerobic and BacT/Alert FN Anaerobic bottles. They achieve faster growth parametres and provide more successful readings of anaerobic bacteria culture and detection.
Cermák
Pavel
P
Institute of Microbiology, Faculty of Medicine, Hradec Králové, Czech Republic. cermakp@lfhk.cuni.cz
Förstl
Miroslav
M
D001421:Bacteria, Anaerobic / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001769:Blood; D003470:Culture Media; D006801:Humans
D003470:Culture Media