title: Pulsed electromagnetic fields reduce knee osteoarthritic lesion progression in the aged Dunkin Hartley guinea pig.
abstract: An experimental in vivo study was performed to test if the effect of Pulsed Electromagnetic Fields (PEMFs) on chondrocyte metabolism and adenosine A2a agonist activity could have a chondroprotective effect on the knee of Dunkin Hartley guinea-pigs of 12 months with spontaneously developed osteoarthritis (OA). After a pilot study, 10 animals were randomly divided into two groups: PEMF-treated group (6 h/day for 3 months) and Sham-treated group. Microradiography and histomorphometry were performed on the entire articular surface of knee joints used in evaluating chondropathy severity, cartilage thickness (CT), cartilage surface Fibrillation Index (FI), subchondral bone plate thickness (SBT) and histomorphometric characteristics of trabecular epiphyseal bone. The PEMF-treated animals showed a significant reduction of chondropathy progression in all knee examined areas (p<0.05). CT was significantly higher (p<0.001) in the medial tibia plateaus of the PEMF-treated group when compared to the Sham-treated group. The highest value of FI was observed in the medial tibia plateau of the Sham-treated group (p<0.05). Significant lower values were observed in SBT of PEMF-treated group in comparison to Sham-treated group in all knee examined areas (p<0.05). The present study results show that PEMFs preserve the morphology of articular cartilage and slower the progression of OA lesions in the knee of aged osteoarthritic guinea pigs. The chondroprotective effect of PEMFs was demonstrated not only in the medial tibial plateau but also on the entire articular surface of the knee.
authors: Department of Experimental Surgery, Codivilla-Putti Research Institute, Rizzoli Institute of Orthopaedics, Via di Barbiano, 1/10, 40136 Bologna, Italy. milena.fini@ior.it
authors: M
authors: M
authors: Fini
authors: G
authors: G
authors: Giavaresi
authors: P
authors: P
authors: Torricelli
authors: F
authors: F
authors: Cavani
authors: S
authors: S
authors: Setti
authors: V
authors: V
authors: Canè
authors: R
authors: R
authors: Giardino
mesh_terms: D000375:Aging; D000818:Animals; D002358:Cartilage, Articular / Q000000981:diagnostic imaging / Q000473:pathology / Q000528:radiation effects; D019902:Chondrocytes / Q000378:metabolism / Q000528:radiation effects*; D018450:Disease Progression; D004599:Electric Stimulation Therapy / Q000379:methods*; D004574:Electromagnetic Fields; D006168:Guinea Pigs; D007719:Knee Joint / Q000000981:diagnostic imaging / Q000378:metabolism / Q000528:radiation effects; D020370:Osteoarthritis, Knee / Q000000981:diagnostic imaging / Q000473:pathology* / Q000628:therapy*; D011859:Radiography; D043705:Receptor, Adenosine A2A / Q000378:metabolism
chemical_list: D043705:Receptor, Adenosine A2A