Analyzing the radiation of the melanocortins in amphibians: cloning of POMC cDNAs from the pituitary of the urodele amphibians, Amphiuma means and Necturus maculosus.
Proopiomelanocortin (POMC) cDNAs were cloned and sequenced from brain extracts of two species of urodele amphibians: Amphiuma means and Necturus maculosus. Although the two species of urodele amphibians belong to separate families, and do not share a direct common ancestor, the level of primary sequence identity for the open reading of the POMC cDNAs was 90% at the amino acid level and 79% at the nucleotide level. It appears that the POMC gene in these urodele amphibians has been accumulating mutations at the amino acid level at a slower rate than the POMC gene in other sarcopterygian orders.
Kozak
Katarzyna
K
University of Denver, Department of Biological Sciences, Denver, CO 80210, USA.
Costantino
David
D
Lecaude
Stephanie
S
Sollars
Cristina
C
Danielson
Phillip
P
Dores
Robert M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009338:Necturus maculosus / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism / Q000528:radiation effects; D011333:Pro-Opiomelanocortin / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014562:Caudata / Q000235:genetics*; D019825:gamma-MSH / Q000235:genetics / Q000528:radiation effects
D019825:gamma-MSH; D011333:Pro-Opiomelanocortin