Thiol specific oxidative stress response in Mycobacteria.
The cellular response of mycobacteria to thiol specific oxidative stress was studied in Mycobacterium bovis BCG cultures. Two-dimensional gel electrophoresis revealed that upon diamide treatment at least 60 proteins were upregulated. Fourteen of these proteins were identified by MALDI-MS; four proteins, AhpC, Tpx, GroEL2, and GroEL1 are functionally related to oxidative stress response; eight proteins, LeuC, LeuD, Rv0224c, Rv3029c, AsnB, Rv2971, PheA and HisH are classified as part of the bacterial intermediary metabolism and respiration pathways; protein EchA14 belong to lipid metabolism, and NrdE, belongs to the mycobacterial information pathway category. Reverse transcription followed by quantitative real time PCR in response to diamide stress demonstrated that protein expression is directly proportional to the corresponding gene transcription.
Dosanjh
Nirpjit S
NS
Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada V5Z 3J5.
Rawat
Mamta
M
Chung
Ji-Hae
JH
Av-Gay
Yossef
Y
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003958:Diamide / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D009163:Mycobacterium bovis / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress; D020543:Proteome; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D020543:Proteome; D013438:Sulfhydryl Compounds; D003958:Diamide