Electrochemical processing of fibrinogen modified-graphite surfaces: effect on plasmin generation from adsorbed plasminogen.
With the aim to improve the fibrinolytic properties of carbons by different biological and electrochemical treatments, we modified graphite surfaces by fibrinogen adsorption and subsequent application of various constant potentials before submitting them to plasminogen adsorption. First, we verified that plasminogen (purified or present in human plasma) could adsorb onto these modified surfaces and that adsorbed plasminogen could be converted by t-PA (the principal physiological activator of plasminogen) to adsorbed plasmin. The catalytic properties of the generated enzyme were characterized in assay solutions containing t-PA, fibrinogen and the chromogenic substrate S-2403 (pyroGlu-Phe-Lys-p-nitroaniline, HCl). Experiments showed that the application of electrical potentials to the fibrinogen coating could indirectly affect the properties of the material. In the case of anodic potentials, the amidolytic activity of the generated plasmin was significantly enhanced. Especially, this activity was 10 times higher at a particular potential value.
Alfonsi-Hourdin
Séverine
S
LECMA, Université de Cergy-Pontoise, 5 Mail Gay Lussac, 95031 Cergy-Pontoise Cedex, France.
Longchamp
Sylvie
S
Gallet
Olivier
O
Nigretto
Jean-Maxime
JM
D000327:Adsorption; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry*; D005341:Fibrinolysin / Q000737:chemistry; D005342:Fibrinolysis; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008422:Materials Testing; D008956:Models, Chemical; D009842:Oligopeptides / Q000494:pharmacology; D010100:Oxygen / Q000737:chemistry*; D010958:Plasminogen / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D009842:Oligopeptides; C110926:S 2403; D002244:Carbon; D006108:Graphite; D005337:Fibrin; D005340:Fibrinogen; D010958:Plasminogen; D005341:Fibrinolysin; D010100:Oxygen