Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.
The present study evaluated the presence of LAT1 and LAT2 amino acid transporters in human Caco-2 cells and rat IEC-6 cells along the mucosa of the rat digestive tract. The LAT1 cDNA was amplified by PCR using two sets of primers (one specific for rat LAT1 and another simultaneously specific for human, rat and mice). The LAT2 cDNA was amplified by PCR using one set of primers simultaneously specific for human, rat and mice LAT2. The presence of LAT1 and LAT2 protein was examined by means of immunoblotting using an antibody raised against the rat LAT1 and mouse LAT2. Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 transporter transcripts and protein. LAT1 protein is most abundant in IEC-6 cells, which is in agreement with functional data previously reported. The findings in the rat intestinal mucosa indicate that LAT1 protein is most abundant in the colon and its abundance markedly decreases at the level of jejunum and ileum, which contrast with relative homogeneous presence of LAT2 across the digestive tract. In conclusion, Caco-2 and IEC-6 cells, as well as the rat intestinal mucosa, are endowed with both LAT1 and LAT2 amino acid transporter transcripts and protein.
Fraga
S
S
Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal.
Pinho
M J
MJ
Soares-da-Silva
P
P
D027182:Amino Acid Transport System y+ / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D018938:Caco-2 Cells; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D027301:Fusion Regulatory Protein 1, Light Chains / Q000096:biosynthesis* / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012313:RNA / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D027182:Amino Acid Transport System y+; D027301:Fusion Regulatory Protein 1, Light Chains; D027283:Large Neutral Amino Acid-Transporter 1; C420646:SLC7A8 protein, human; C486582:Slc7a8 protein, rat; D012313:RNA