Activities of fresh juice of Scutellaria barbata and warmed water extract of Radix Sophorae Tonkinensis on anti-proliferation and apoptosis of human cancer cell lines.
The possible antiproliferative and apoptotic inducing potentials of fresh juice prepared from Scutellaria barbata (SBJ) and warmed water extract of Radix Sophorae Tonkinensis (RSTE) have been tested on a series of cancer cell lines, including HepG2 hepatoblastoma, Hep3B hepatocellular carcinoma, MDA-MB231 breast carcinoma, A549 lung cancer and KG-1 acute myelogenous leukaemia in vitro. Both SBJ and RSTE were able to inhibit the growth of cancer cell lines and induce apoptosis. Further analysis of the action of RSTE on HepG2 cells suggested that the activity of the central machinery of apoptosis, caspase 3, was significantly elevated. Oligo-nucleosomal length DNA fragments formation was readily detected by TdT-mediated dUTP nick end labelling assay after RSTE treatment. Taken together, we believe that, although Radix Sophorae Tonkinensis was demonstrated to have toxic components including matrine and oxymatrine, it is still worthwhile to further investigate its anti-cancer potential under a safety toxicological precaution.
Chui
Chung Hin
CH
Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong Polytechnic University, Hong Kong, P.R. China.
Lau
Fung Yi
FY
Tang
Johnny Cheuk On
JC
Kan
Kin Luen
KL
Cheng
Gregory Yin Ming
GY
Wong
Raymond Siu Ming
RS
Kok
Stanton Hon Lung
SH
Lai
Paul Bo San
PB
Ho
Rocky
R
Gambari
Roberto
R
Chan
Albert Sun Chi
AS
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001628:Beverages; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007887:Fabaceae; D006801:Humans; D020287:In Situ Nick-End Labeling; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D031363:Scutellaria; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014867:Water; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases