title: Calorie restriction delays lipid oxidative damage in Drosophila melanogaster.
abstract: The oxidative stress hypothesis predicts that the accumulation of oxidative damage to a variety of macromolecules is the molecular trigger driving the process of aging. Although an inverse relationship between oxidative damage and lifespan has been established in several different species, the precise relationship between oxidative damage and aging is not fully understood. Drosophila melanogaster is a favored model organism for aging research. Environmental interventions such as ambient temperature and calorie restriction can alter adult lifespan to provide an excellent system to examine the relationship between oxidative damage, aging and lifespan. We have developed an enzyme-linked immunosorbent assay (ELISA) using commercially available reagents for measuring 4-hydroxy-2-nonenal (HNE) in proteins, a marker for oxidative damage to lipids, and present data in flies to show that HNE adducts accumulate in an age-dependent manner. With immunohistology, we also find the primary site of HNE accumulation is the pericerebral fat body, where induction of dFOXO was recently shown to retard aging. When subjected to environmental interventions that shorten lifespan, such as elevated ambient temperature, the chronological accumulation of HNE adduct is accelerated. Conversely, interventions that extend lifespan, such as lower ambient temperature or low calorie diets, slow the accumulation of HNE adduct. These studies associate damage from lipid peroxidation with aging and lifespan in Drosophila and show that calorie restriction in flies, as in mammals, slows the accumulation of lipid related oxidative damage.
authors: University of Connecticut Health Center, Department of Genetics and Developmental Biology, Farmington, CT 06030, USA.
authors: Jianyu
authors: J
authors: Zheng
authors: Raye
authors: R
authors: Mutcherson
authors: Stephen L
authors: SL
authors: Helfand
mesh_terms: D000375:Aging / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D031204:Caloric Restriction; D004331:Drosophila melanogaster / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005216:Fat Body / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008136:Longevity / Q000187:drug effects / Q000502:physiology*; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010269:Paraquat / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D024101:Mitochondrial Proteins; C462359:tert-4-hydroxy-2-nonenal; D010269:Paraquat