title: Subcellular localization of the Triple Gene Block movement proteins of Beet necrotic yellow vein virus by electron microscopy.
abstract: The Triple Gene Block proteins TGBp1, TGBp2, and TGBp3 of Beet necrotic yellow vein virus (BNYVV) are required for efficient cell-to-cell spread of the infection. The TGB proteins can drive cell-to-cell movement of BNYVV in trans when expressed from a co-inoculated BNYVV RNA 3-based 'replicon'. TGBp2 and TGBp3 expressed from the replicon were nonfunctional in this assay if they were fused to the green fluorescent protein (GFP), but addition of a hemagglutinin (HA) tag to their C-termini did not incapacitate movement. Immunogold labeling of ultrathin sections treated with HA-specific antibodies localized TGBp2-HA and TGBp3-HA to what are probably structurally modified plasmodesmata (Pd) in infected cells. A similar subcellular localization was observed for TGBp1. Large gold-decorated membrane-rich bodies containing what appear to be short fragments of endoplasmic reticulum were observed near the cell periphery. The modified gold-decorated Pd and the membrane-rich bodies were not observed when the TGB proteins were produced individually in infections using the Tobacco mosaic virus P30 protein to drive cell-to-cell movement, indicating that these modifications are specific for TGB-mediated movement.
authors: Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, 67084 Strasbourg, France.
authors: M
authors: M
authors: Erhardt
authors: G
authors: G
authors: Vetter
authors: D
authors: D
authors: Gilmer
authors: S
authors: S
authors: Bouzoubaa
authors: K
authors: K
authors: Richards
authors: G
authors: G
authors: Jonard
authors: H
authors: H
authors: Guilley
mesh_terms: D027461:Beta vulgaris / Q000821:virology; D005814:Genes, Viral; D017793:Luteovirus / Q000145:classification / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D009068:Movement; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D014764:Viral Proteins / Q000502:physiology
chemical_list: D014764:Viral Proteins