title: Evaluation of the anticocaine monoclonal antibody GNC92H2 as an immunotherapy for cocaine overdose.
abstract: The illicit use of cocaine continues in epidemic proportions and treatment for cocaine overdose remains elusive. Current protein-based technology offers a new therapeutic venue by which antibodies bind the drug in the blood stream, inactivating its toxic effects. The therapeutic potential of the anticocaine antibody GNC92H2 was examined using a model of cocaine overdose. Swiss albino mice prepared with intrajugular catheters were tested in photocell cages after administration of 93 mg/kg (LD50) of cocaine and GNC92H2 infusions ranging from 30 to 190 mg/kg. GNC92H2 was delivered 30 min before, concomitantly or 3 min after cocaine treatment. Significant blockade of cocaine toxicity was observed with the higher dose of GNC92H2 (190 mg/kg), where premorbid behaviors were reduced up to 40%, seizures up to 77% and death by 72%. Importantly, GNC92H2 prevented death even post-cocaine injection. The results support the important potential of GNC92H2 as a therapeutic tool against cocaine overdose.
authors: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road (BCC 582), La Jolla, CA 92037, United States. rcarrera@scripps.edu
authors: M Rocío A
authors: MR
authors: Carrera
authors: José Manuel
authors: JM
authors: Trigo
authors: Peter
authors: P
authors: Wirsching
authors: Amanda J
authors: AJ
authors: Roberts
authors: Kim D
authors: KD
authors: Janda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D003042:Cocaine / Q000276:immunology / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D062787:Drug Overdose / Q000401:mortality / Q000628:therapy; D007167:Immunotherapy / Q000379:methods; D008297:Male; D051379:Mice; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control; D015996:Survival Rate; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D003042:Cocaine