title: A novel canine model of spinal cord ischemia with reproducible neurologic outcomes.
abstract: OBJECTIVE
  To develop a canine model of spinal cord ischemia (SCI) with highly reproducible neurologic outcomes.
  
  
  METHODS
  Spinal cord ischemia was induced by cross-clamping the proximal descending aorta. To produce substantial ischemia in the critical lumbar region, the proximal aortic blood pressure (PAP) was reduced to 80 mmHg by withdrawing blood into a reservoir connected to the left subclavian artery. We conducted an intraischemia spinal cord electrophysiologic study and a postischemia assessment of hindlimb motor function in six animals subjected to this procedure with an aortic occlusion time of 40 min, and in six animals subjected only to aortic occlusion for 60 min.
  
  
  RESULTS
  All the animals subjected to this procedure exhibited a significant decrease in motor-evoked spinal cord potentials to transcranial electric stimulation (MEPs) during the acute ischemic phase, and they were paraplegic 48 h after ischemia. In contrast, two of the animals not subjected to PAP reduction showed complete functional recovery with intact MEP findings.
  
  
  CONCLUSIONS
  This model is feasible for experimental SCI studies because it can reliably and easily reproduce substantial ischemia.
authors: Department of Surgery, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima 734-8551, Japan.
authors: Yuji
authors: Y
authors: Sugawara
authors: Hajime
authors: H
authors: Kumagai
authors: Taijiro
authors: T
authors: Sueda
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery; D003250:Constriction; D004285:Dogs; D004558:Electric Stimulation; D004568:Electrodiagnosis; D019054:Evoked Potentials, Motor; D023421:Models, Animal; D017063:Outcome Assessment, Health Care; D010264:Paraplegia / Q000209:etiology*; D020127:Recovery of Function; D020760:Spinal Cord Ischemia / Q000150:complications* / Q000175:diagnosis / Q000628:therapy