[Development of transformation system of rice based on transformation-competent artificial chromosome (TAC) vector].
The TAC clone (NK15) containing a ca.50 kb DNA insert was introduced into Agrobacterium tumefaciens strain LBA4404 by electroporation. The NK15 was stable in Agrobacterium tumefaciens strain LBA4404 under kanamycin selection for many generations. The calli of mature embryo of Nongken58S were infected with the Agrobacterium tumefaciens strain LBA4404 carrying NK15. PCR and Southern analyses of transgenic plants indicated that the 50 kb of foreign DNA was transferred into the rice genome, and most of transgenic plants had one copy of the insertion. Genetic and PCR analyses of T1 progeny confirmed that the inserted forgein DNA was stably inherited.
Zhou
Ling-Yan
LY
Genetic Engineeering Laboratory, College of Life Science, South China Agricutural University, Guangzhou 510642, China.
Jiang
Da-Gang
DG
Wu
Hao
H
Zhuang
Chu-Xiong
CX
Liu
Yao-Guang
YG
Mei
Man-Tong
MT
D015139:Blotting, Southern; D022201:Chromosomes, Artificial; D018744:DNA, Plant / Q000235:genetics*; D018274:Electroporation; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D018744:DNA, Plant