Multicomponent phenol hydroxylase-catalysed formation of hydroxyindoles and dyestuffs from indole and its derivatives.
OBJECTIVE
To establish multicomponent phenol hydroxylases (mPHs) as novel biocatalysts for producing dyestuffs and hydroxyindoles such as 7-hydroxyindole (7-HI) from indole and its derivatives.


RESULTS
We have isolated Pseudomonas sp. KL33, which possesses a phenol degradation pathway similar to that found in Pseudomonas sp. CF600. Pseudomonas sp. KL28 is a strain that can grow on n-alkylphenols as a carbon and energy source. Escherichia coli strains expressing mPH from strain KL28 (mPH(KL28)) and strain KL33 (mPH(KL33)) catalysed the formation of indigo and 7-HI, respectively, from indole. In addition, both mPHs catalysed the production of dyestuffs and hydroxyindoles from indole derivatives. The mPH(KL28) has proved to be one of the most versatile biocatalysts that can accommodate a wide range of indole derivatives for catalysing the formation of dyestuffs.


CONCLUSIONS
The present work provides a new approach in producing various dyestuffs and hydroxyindoles from indole and its derivatives by mPHs.


CONCLUSIONS
These results indicate that mPHs may serve as potential agents for organic syntheses as well as bioremediation.
Kim
J Y
JY
Department of Microbiology, Changwon National University, Changwon, Kyongnam, Korea.
Kim
J-K
JK
Lee
S O
SO
Kim
C-K
CK
Lee
K
K
D001711:Biotransformation; D002384:Catalysis; D004396:Coloring Agents / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D007211:Indoles / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
C494864:7-hydroxyindole; D004396:Coloring Agents; D007211:Indoles; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase