title: Quantifying uncertainty associated with microbial count data: a Bayesian approach.
abstract: We consider the problem of estimating bacterial concentration in a substance, given microbial count data. A Bayesian approach is proposed which naturally allows the incorporation of both plate-count data and extra information from confirmatory tests such as genotyping by polymerase chain reaction (PCR). The estimation methods yield posterior credible regions for bacterial concentration, in contrast to the previous methods, which generally only produce point estimates. The approach is illustrated with specific reference to the enumeration of the food-borne pathogen Escherichia coli O157 by spiral plating, although the methodology can be applied to any bacterium or counting method of interest. The results obtained provide guidance to the experimenter as to the number of confirmatory tests which should be performed, and also suggest that in the initial plate count one should err on the side of including rather than excluding colonies whose genotype seems unclear.
authors: Department of Veterinary Clinical Sciences, University of Liverpool, Leahurst, Neston, South Wirral, CH64 7TE, U.K.
authors: H E
authors: HE
authors: Clough
authors: D
authors: D
authors: Clancy
authors: P D
authors: PD
authors: O'Neill
authors: S E
authors: SE
authors: Robinson
authors: N P
authors: NP
authors: French
mesh_terms: D001499:Bayes Theorem; D015169:Colony Count, Microbial; D003627:Data Interpretation, Statistical; D019453:Escherichia coli O157 / Q000378:metabolism; D005516:Food Microbiology; D005838:Genotype; D008829:Microbiology / Q000592:standards; D015233:Models, Statistical; D016133:Polymerase Chain Reaction / Q000379:methods; D012107:Research Design; D012306:Risk; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D013223:Statistics as Topic / Q000379:methods; D035501:Uncertainty