title: Novel bactericidal surface: Catechin-loaded surface-erodible polymer prevents biofilm formation.
abstract: We developed a novel bactericidal surface based on a catechin-loaded surface-erodible polymer. (-)-Epigallocatechin-3-gallate (EGCg), which is the main constituent of tea catechins, showed a dose-dependent inhibitory effect on Escherichia coli biofilm formation and a dose-dependent enhanced destructive effect on biofilm. EGCg-immobilized surfaces were prepared by photopolymerization of liquid biodegradable polyesters. The releasing rate was enhanced with an increase in surface-erosion rate of photocured polymers. Polymers with high releasing capacity dose-dependently reduced biofilm formation on the surfaces. The confocal laser scanning microscopic and scanning electron microscopic observations revealed that EGCg induced biofilm-destructing activities, which include bacterial membrane damage, degradation of exopolysaccharides, and detachment of colonized cells. From these results, potential advantages of the clinical use of catechin-loaded polymer-coated implants or catheters are discussed in terms of a reduced occurrence of biomaterial-centered infections without substantial toxicity or adverse effects.
authors: Division of Biomedical Engineering, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Ryo
authors: R
authors: Maeyama
authors: Il Keun
authors: IK
authors: Kwon
authors: Yoshimitsu
authors: Y
authors: Mizunoe
authors: James M
authors: JM
authors: Anderson
authors: Masao
authors: M
authors: Tanaka
authors: Takehisa
authors: T
authors: Matsuda
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D018441:Biofilms; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D020128:Inhibitory Concentration 50; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011108:Polymers; D011134:Polysaccharides; D002392:Catechin