Non protein bound iron concentrations in amniotic fluid.
OBJECTIVE
To investigate whether amniotic fluid concentrations of non protein bound iron (NPBI) vary with growth in healthy fetuses and also offer a reference curve in the second trimester of pregnancy.


METHODS
Amniotic fluid concentrations of NPBI were measured by HPLC in 118 women with physiological singleton pregnancies, who underwent amniocentesis for fetal karyotype between weeks 15 and 18 of gestation.


RESULTS
NPBI increased progressively from weeks 14--15 to weeks 15--16, peaking at 17--18 weeks of gestation. NPBI values regressed positively with gestational age (GA). Multiple linear regression analysis between NPBI, as dependent variable, and various fetal parameters, as independent variables, showed a statistically significant regression coefficient with GA, bi-parietal diameter and transverse cerebellar diameter.


CONCLUSIONS
The present data constitutes the first quantification of NPBI concentrations in amniotic fluid under physiological conditions. Correlations with GA and ultrasound fetal biometry suggest that NPBI may play a role in fetal growth.
Gazzolo
D
D
Department Paediatrics G. Gaslini, Children's University Hospital, 16148 Genoa, Italy.
Perrone
S
S
Paffetti
P
P
Longini
M
M
Vezzosi
P
P
Bruschettini
M
M
Lituania
M
M
Buonocore
G
G
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007501:Iron / Q000032:analysis* / Q000737:chemistry; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000502:physiology; D000509:alpha-Fetoproteins / Q000032:analysis
D000509:alpha-Fetoproteins; D007501:Iron; D009571:Nitrilotriacetic Acid