title: Identification of enterococcal, streptococcal and Weissella species in the faecal flora of individually owned dogs.
abstract: OBJECTIVE
  To improve the limited information on the composition of the faecal Gram-positive coccal flora of healthy dogs by the use of a molecular identification method.
  
  
  RESULTS
  Faecal swabs were collected for the selective isolation of Gram-positive coccal strains. Colonies with enterococcal- and streptococcal-like morphology were identified by tRNA intergenic length polymorphism analysis (tDNA-PCR). Fourteen known species belonging to three genera (Enterococcus, Streptococcus and Weissella) and one alleged new enterococcal species were found.
  
  
  CONCLUSIONS
  The faecal flora of dogs comprises an unusually broad diversity of culturable Gram-positive coccal species with Enterococcus faecalis being most frequently present followed by not less than six other species of about equal importance.
  
  
  CONCLUSIONS
  Many human- and animal-associated enterococci and streptococci are also present in dog faeces together with the largely uncharacterized Weissella cibaria and a group of strains resembling Enterococcus dispar, but representing a distinct and hitherto unknown species. Phenotypic characteristics of the latter two species were determined and the test results were compared with the species descriptions of W. cibaria and E. dispar respectively.
authors: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. evelyne.degraef@ugent.be
authors: E M
authors: EM
authors: Graef
authors: L A
authors: LA
authors: Devriese
authors: M
authors: M
authors: Baele
authors: M
authors: M
authors: Vancanneyt
authors: J
authors: J
authors: Swings
authors: F
authors: F
authors: Haesebrouck
authors: A
authors: A
authors: Decostere
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016983:Enterococcus / Q000235:genetics / Q000302:isolation & purification*; D013293:Enterococcus faecalis / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000032:analysis; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer