Relationship between antitumor efficiency of photodynamic therapy with photoditasine and photoenergy density.
We studied the effect of photodynamic therapy with photoditasine at different protocols of photoenergy exposure on morphofunctional parameters of M-1 sarcoma. It was found that proliferative activity of tumor cells (evaluated by immunostaining for PCNA) nonlinearly decreases after exposure to 150, 300, and 600 J/cm2. The main form of cell death during the early period after photodynamic therapy was direct photocoagulation necrosis caused by destruction of sensitized cell structures and ischemic necrosis developing as a result of alteration of vascular network in the tumors. Photoenergy density was not essential for the intensity of induced apoptosis.
Romanko
Yu S
YS
Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk, Russia. y_romanko@hotmail.com
Tsyb
A F
AF
Kaplan
M A
MA
Popuchiev
V V
VV
D000818:Animals; D008297:Male; D008536:Meglumine / Q000627:therapeutic use*; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D051381:Rats; D012513:Sarcoma, Experimental / Q000188:drug therapy*
D017319:Photosensitizing Agents; D008536:Meglumine