Use of nano-liquid chromatography for the analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and candies.
Glycyrrhizin (G) and glycyrrhetic acid (GA) were separated by using nano-liquid chromatography (nano-LC) in a fused silica capillary packed with RP18 stationary phase (75 microm ID, effective length 33 cm, packed 23 cm) eluting at 300 nL/min in a gradient mode. The mobile phase was a mixture of water-MeOH-MeCN-acetic acid (29:35:35:1, v/v/v/v) that was delivered for one minute and after this was modified by reducing the water content (14:42.5:42.5:1, v/v/v/v). The intra-day and inter-day relative standard deviations (of retention time and peak area) were satisfactory (lower than 2.9 and 4%, respectively). The linearity of the nano-LC method was assessed in the range 0.62-5.00 microg/mL and 80-200 microg/mL for GA and G, with R2 = 0.996 and 0.995, respectively. The licorice was extracted with a mixture of ethanol-water, diluted with the mobile phase, and injected for the analysis.
Fanali
Salvatore
S
Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma 1, Monterotondo Scalo (Roma), Italy. fanali@imc.cnr.it
Aturkil
Zeineb
Z
D'Orazio
Giovanni
G
Raggi
Maria Augusta
MA
Quaglia
Maria Giovanna
MG
Sabbioni
Cesare
C
Rocco
Anna
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006034:Glycyrrhetinic Acid / Q000032:analysis*; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis*; D036103:Nanotechnology; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid