title: Fibroblast growth factor 8 regulates neocortical guidance of area-specific thalamic innervation.
abstract: Thalamic innervation of each neocortical area is vital to cortical function, but the developmental strategies that guide axons to specific areas remain unclear. We took a new approach to determine the contribution of intracortical cues. The cortical patterning molecule fibroblast growth factor 8 (FGF8) was misexpressed in the cortical primordium to rearrange the area map. Thalamic axons faithfully tracked changes in area position and innervated duplicated somatosensory barrel fields induced by an ectopic source of FGF8, indicating that thalamic axons indeed use intracortical positional information. Because cortical layers are generated in temporal order, FGF8 misexpression at different ages could be used to shift regional identity in the subplate and cortical plate either in or out of register. Thalamic axons showed strikingly different responses in the two different conditions, disclosing sources of positional guidance in both subplate and cortical plate. Unexpectedly, axon trajectories indicated that an individual neocortical layer could provide not only laminar but also area-specific guidance. Our findings demonstrate that thalamocortical axons are directed by sequential, positional cues within the cortex and implicate FGF8 as an indirect regulator of thalamocortical innervation.
authors: Department of Neurobiology, Pharmacology, and Physiology, University of Chicago, Chicago, Illinois 60637, USA.
authors: Tomomi
authors: T
authors: Shimogori
authors: Elizabeth A
authors: EA
authors: Grove
mesh_terms: D000818:Animals; D001369:Axons / Q000648:ultrastructure; D018274:Electroporation; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D007276:Injections, Intraventricular; D051379:Mice; D009434:Neural Pathways / Q000196:embryology / Q000254:growth & development; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013003:Somatosensory Cortex / Q000196:embryology / Q000254:growth & development; D013687:Telencephalon / Q000196:embryology / Q000254:growth & development; D013788:Thalamus / Q000196:embryology* / Q000254:growth & development; D014162:Transfection; D014738:Vibrissae / Q000294:innervation
chemical_list: C496457:Fgf8 protein, mouse; D011993:Recombinant Fusion Proteins; D051524:Fibroblast Growth Factor 8; C496365:FGFR3 protein, human; D051498:Receptor, Fibroblast Growth Factor, Type 3