title: Mutations in the gyrA and parC genes in ciprofloxacin-resistant clinical isolates of Acinetobacter baumannii in Korea.
abstract: Mutation with Ser-83-->Leu in gyrA gene was associated with the principal mutation for ciprofloxacin resistance in clinical isolates of Acinetobacter baumannii. Double mutation, Ser-83-->Leu in gyrA gene and Ser-80-->Leu in parC gene, was the most frequently detected among ciprofloxacin-resistant isolates. A novel mutation with Ser-80-->Trp in parC gene, in addition to mutation in gyrA gene, was associated with a high-level ciprofloxacin resistance. These results suggested that the presence of an additional mutation in the parC gene contributed to a higher-level of ciprofloxacin resistance than a single mutation in the gyrA gene (geometric mean MICs of ciprofloxacin, 44.1 versus 16 microg/ml, P < 0.05).
authors: Division of Antimicrobial Resistance Pathogens, Department of Bacteriology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul.
authors: Jeom Kyu
authors: JK
authors: Lee
authors: Yeong Seon
authors: YS
authors: Lee
authors: Yong Keun
authors: YK
authors: Park
authors: Bong Su
authors: BS
authors: Kim
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010802:Phylogeny
chemical_list: D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase