title: Training enhances ANP lipid-mobilizing action in adipose tissue of overweight men.
abstract: OBJECTIVE
  This study was designed to evaluate whether a 4-month endurance training program could improve ANP- as well as isoproterenol-mediated (beta-adrenergic receptor agonist) in situ lipolysis and adipose tissue blood flow (ATBF) in the subcutaneous adipose tissue (SCAT) of untrained overweight subjects.
  
  
  METHODS
  Ten overweight men aged 26.0 +/- 1.4 yr with a mean body mass index of 27.6 +/- 0.2 kg.m(-2), performed aerobic exercise 5 d.wk(-1) for 4 months. Before and after the training period, SCAT responsiveness was investigated in situ during a 60-min infusion of 1 micromol.L(-1) isoproterenol and 10 micromol.L(-1) ANP through microdialysis probes. Plasma metabolic parameters and physical fitness variables were measured as well.
  
  
  RESULTS
  Endurance training significantly increased fat-free mass and VO2max, while reducing plasma insulin, glucose, NEFA, low density lipoprotein (LDL)-C and the respiratory exchange ratio at rest. Training significantly lowered resting dialysate glycerol levels in SCAT. The lipid-mobilizing effect of ANP was markedly enhanced (by 191%, P < 0.05) after training as was that of isoproterenol (by 145%, P < 0.05). Resting adipose tissue blood flow as well as ANP- and isoproterenol-mediated rise in ATBF was increased after training.
  
  
  CONCLUSIONS
  The present study shows that endurance training improves ANP- as well as beta-adrenergic-receptor-mediated lipid mobilization and ATBF in the SCAT of overweight subjects. The recovery of a higher lipolytic efficiency in adipose tissue is an important benefit of a training program in overweight subjects.
authors: Franco-Czech Laboratory for Clinical Research on Obesity, Inserm U586, Institut Louis Bugnard, Centre Hospitalier Universitaire de Toulouse, Université Paul Sabatier, Toulouse, France.
authors: Cedric
authors: C
authors: Moro
authors: Fabien
authors: F
authors: Pillard
authors: Isabelle
authors: I
authors: De Glisezinski
authors: Isabelle
authors: I
authors: Harant
authors: Daniel
authors: D
authors: Rivière
authors: Vladimir
authors: V
authors: Stich
authors: Max
authors: M
authors: Lafontan
authors: François
authors: F
authors: Crampes
authors: Michel
authors: M
authors: Berlan
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply* / Q000187:drug effects; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000328:Adult; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D005602:France; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008053:Lipid Mobilization / Q000502:physiology*; D008297:Male; D017551:Microdialysis; D009765:Obesity
chemical_list: D000318:Adrenergic beta-Agonists; D009320:Atrial Natriuretic Factor; D007545:Isoproterenol