Comparative characterization of cytokine production by concanavalin A-activated splenocytes from BALB/c and C57BL/6 mice after cold exposure.
The level of cytokines produced by ConA activated splenocytes was studied in male BALB/c and C57Bl/6 mice after single and repeated cold exposure (-20 degrees C, 3 min). Single cold exposure significantly decreased IL-2, -3, -4, -5, -10, -12, IFN-gamma production in BALB/c mice and decreased IL-2 content and increased TNF-alpha level in C57Bl/6 mice. Repeated cold exposure normalized the content of IL-2, -4, -10, -12, and IFN-gamma in BALB/c mice, which reflects the development of adaptive immune reactions. In C57Bl/6 mice IL-2, -3, -5, -10, -12, and IFN-gamma production remained significantly decreased, which attested to dysadaptive processes.
Makarova
O V
OV
Laboratory of Inflammation Immunomorphology, Institute of Human Morphology, Russian Academy of Medical Sciences, Moscow. tgv@shchyolkovo.ru
Trunova
G V
GV
Diatroptov
M E
ME
Serebryakov
S N
SN
Kondashevskaya
M V
MV
Malaitsev
V V
VV
D000818:Animals; D003080:Cold Temperature; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D003208:Concanavalin A