Markers of cellular adhesion in diagnosis and therapy control of colorectal carcinoma.
OBJECTIVE
Early diagnosis of the progressive tumor disease and control of the effect of therapy in colorectal carcinoma are most frequently performed by monitoring CEA or CA 19-9 tumor markers. Their clinical application is, however, limited. The aim of our study was to demonstrate the contribution of adhesive molecule assessment to the early diagnosis of progression. We also wanted to find out if changes in the levels of cellular adhesion parameters correlate with the effect of antitumor therapy.


METHODS
Intercellular cell adhesive molecule-1 (ICAM-1) and Vascular cell adhesive molecule-1 (VCAM-1) were assessed using the ELISA method, and the results were correlated with CEA and CA 19-9 tumor markers. Three hundred and sixty-four patients with colorectal carcinoma in Dukes' stages B-D were monitored. The results were processed with the SAS 6.2. statistical program and Statistica.


RESULTS
In 92 patients with first clinical progression (occurrence of distant metastases irrespective of localization), significantly increased ICAM-1 and VCAM-1 values were demonstrated. In ROC evaluation of curves, we also demonstrated high sensitivity of adhesive molecules against both the control healthy group (n =89) and the no evidence of disease group (NED) (n=183). Adhesive molecule levels were closely connected with the type and course of therapy and are presented in the form of case reports.


CONCLUSIONS
Soluble adhesive molecules are a prospective parameter both for the early diagnosis of progression and for control of the effect of therapy. There is a need for a large-scale study, preferably multicentric, which would verify the suitability of introducing cellular adhesion parameter assessment into routine practice.
Holubec
L
L
Charles University Prague, Medical Faculty Pilsen, Czech Republic. HOLUBEC@fnplzen.cz
Topolcan
O
O
Finek
J
J
Holdenrieder
S
S
Stieber
P
P
Pesta
M
M
Pikner
R
R
Holubec Sen
L
L
Sutnar
A
A
Liska
V
V
Svobodova
S
S
Visokai
V
V
Kormunda
S
S
D014408:Biomarkers, Tumor / Q000097:blood*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000097:blood; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules