Aptamer-based biosensors for the detection of HIV-1 Tat protein.
Two biosensors have been constructed using an RNA aptamer as biorecognition element. The aptamer, specific for HIV-1 Tat protein, has been immobilised on the gold surface of piezoelectric quartz crystals or surface plasmon resonance (SPR) chips to develop a quartz crystal microbalance (QCM)-based and an SPR-based biosensor, respectively. Both the biosensors were modified with the same immobilisation chemistry based on the binding of a biotinylated aptamer on a layer of streptavidin. The binding between the immobilised aptamer and its specific protein has been evaluated with the two biosensors in terms of sensitivity, reproducibility and selectivity. A protein very similar to Tat, Rev protein, has been used as negative control. The two biosensors both were very reproducible in the immobilisation and the binding steps. The selectivity was high in both cases.
Tombelli
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Università degli Studi di Firenze, Dipartimento di Chimica Polo Scientifico di Sesto Fiorentino Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.
Minunni
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M
Luzi
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E
Mascini
M
M
D015374:Biosensing Techniques; D015696:Gene Products, tat / Q000032:analysis* / Q000737:chemistry; D015497:HIV-1; D011791:Quartz / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus; D011791:Quartz; D012313:RNA