title: Fixation of conserved sequences shapes human intron size and influences transposon-insertion dynamics.
abstract: The basis for intron expansion in humans is largely unexplored. In this article, we demonstrate that intron expansion has primarily been determined by fixation of multispecies conserved sequences (MCSs) over time. The presence of MCSs has shaped intron features: the insertion of transposable elements (TEs) has been constrained as more MCSs were fixed. Analysis of TE and MCS distribution suggested an unprecedented estimate of information requirements for proper splicing of long introns with indication of sequence constraints extending up to >3 kb downstream 5' splice sites.
authors: Scientific Institute IRCCS E, Medea, Via Don Luigi Monza 20, 23842 Bosisio Parini (LC), Italy.
authors: Manuela
authors: M
authors: Sironi
authors: Giorgia
authors: G
authors: Menozzi
authors: Giacomo P
authors: GP
authors: Comi
authors: Nereo
authors: N
authors: Bresolin
authors: Rachele
authors: R
authors: Cagliani
authors: Uberto
authors: U
authors: Pozzoli
mesh_terms: D017398:Alternative Splicing; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D005091:Exons; D015894:Genome, Human; D006801:Humans; D007438:Introns
chemical_list: D004251:DNA Transposable Elements