Genomic analysis of Grapevine Retrotransposon 1 (Gret 1) in Vitis vinifera.
The complete sequence of the first retrotransposon isolated in Vitis vinifera, Gret 1, was used to design primers that permitted its analysis in the genome of grapevine cultivars. This retroelement was found to be dispersed throughout the genome with sites of repeated insertions. Fluorescent in situ hybridization indicated multiple Gret 1 loci distributed throughout euchromatic portions of chromosomes. REMAP and IRAP proved to be useful as molecular markers in grapevine. Both of these techniques showed polymorphisms between cultivars but not between clones of the same cultivar, indicating differences in Gret 1 distribution between cultivars. The combined cytological and molecular results suggest that Gret 1 may have a role in gene regulation and in explaining the enormous phenotypic variability that exists between cultivars.
Pereira
H Sofia
HS
Secção de Genética, Instituto Superior de Agronomia, Centro de Botânica Aplicada à Agricultura, Tapada da Ajuda, 1349-017 Lisboa, Portugal. sofiapereira@isa.utl.pt
Barão
Augusta
A
Delgado
Margarida
M
Morais-Cecílio
Leonor
L
Viegas
Wanda
W
D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008677:Metaphase; D018517:Plant Roots / Q000166:cytology / Q000235:genetics; D015183:Restriction Mapping; D018626:Retroelements; D027843:Vitis / Q000235:genetics*
D017931:DNA Primers; D018626:Retroelements