Novel agmatine-containing poly(amidoamine) hydrogels as scaffolds for tissue engineering.
Novel biocompatible and biodegradable amphoteric poly(amidoamine) (PAA) hydrogels were designed for applications as scaffolds for tissue engineering. These hydrogels (PAA-AG1 and PAA-AG2) were obtained by polyaddition of 2,2-bisacrylamidoacetic acid with 2-methylpiperazine and 4-aminobutyl guanidine, a bioactive molecule with a known ability to induce adhesion to cell membranes. They contain carboxylic functions in their main chain and interchain connections deriving from two different cross-linking agents: for PAA-AG1, a multifunctional primary amine, that is, 1,10-decanediamine; for PAA-AG2, a purposely synthesized PAA (PAA-NH(2)) containing pendant NH(2). Both PAA-AG1 and PAA-AG2 proved noncytotoxic and adhesive to cell membranes, as ascertained by means of cytotoxicity and proliferation tests carried out on fibroblast cell lines. Good apparent mechanical strength was also observed in the case of PAA-AG2, cross-linked with the PAA-NH(2). Both PAA-AG1 and PAA-AG2 underwent degradation tests under controlled conditions simulating the biological environments, that is, Dulbecco medium at pH 7.4 and 37 degrees C. They completely dissolved within 10 and about 40 days, respectively. In both cases, the degradation products were completely noncytotoxic. All the results of this paper point to the conclusion that agmatine-based PAA hydrogels are excellent substrates for cell proliferation.
Ferruti
Paolo
P
Dipartimento di Chimica Organica e Industriale, Università di Milano, via Venezian 21, 20133 Milano, Italy. paolo.ferruti@unimi.it
Bianchi
Sabrina
S
Ranucci
Elisabetta
E
Chiellini
Federica
F
Piras
Anna Maria
AM
D016475:3T3 Cells; D000376:Agmatine / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D009757:Nylons / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D023822:Tissue Engineering
D020100:Hydrogels; D009757:Nylons; D011073:Polyamines; D000376:Agmatine