Pulmonary exposure to diesel exhaust particles induces airway inflammation and cytokine expression in NC/Nga mice.
Although several studies have reported that diesel exhaust particles (DEP) affect cardiorespiratory health in animals and humans, the effect of DEP on animal models with spontaneous allergic disorders has been far less intensively studied. The Nc/Nga mouse is known to be a typical animal model for human atopic dermatitis (AD). In the present study, we investigated the effects of repeated pulmonary exposure to DEP on airway inflammation and cytokine expression in NC/Nga mice. The animals were randomized into two experimental groups that received vehicle or DEP by intratracheal instillation weekly for six weeks. Cellular profiles of bronchoalveolar lavage (BAL) fluid and expressions of cytokines and chemokines in both the BAL fluid and lung tissues were evaluated 24 h after the last instillation. The DEP challenge produced an increase in the numbers of total cells, neutrophils, and mononuclear cells in BAL fluid as compared to the vehicle challenge (P<0.01). DEP exposure significantly induced the lung expressions of interleukin (IL)-4, keratinocyte chemoattractant (KC), and macrophage inflammatory protein (MIP)-1alpha when compared to the vehicle challenge. These results indicate that intratracheal exposure to DEP induces the recruitment of inflammatory cells, at least partially, through the local expression of IL-4 and chemokines in NC/Nga mice.
Inoue
Ken-ichiro
K
Inhalation Toxicology and Pathophysiology Research Team, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan.
Takano
Hirohisa
H
Yanagisawa
Rie
R
Ichinose
Takamichi
T
Shimada
Akinori
A
Yoshikawa
Toshikazu
T
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005742:Gasoline / Q000633:toxicity*; D007249:Inflammation / Q000209:etiology*; D015847:Interleukin-4 / Q000378:metabolism; D007442:Intubation, Intratracheal; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils; D011897:Random Allocation; D012137:Respiratory System / Q000187:drug effects* / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D005742:Gasoline; D019402:Macrophage Inflammatory Proteins; D001335:Vehicle Emissions; D015847:Interleukin-4