One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch. using high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale has been successfully applied to the separation of bioactive flavonoid compounds, liquiritigenin and isoliquiritigenin in one step from the crude extract of Glycyrrhiza uralensis Risch. The HSCCC was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (2:2:1:0.6:2, v/v). Yields of liquiritigenin (98.9% purity) and isoliquiritigenin (98.3% purity) obtained were 0.52% and 0.32%. Chemical structures of the purified liquiritigenin and isoliquiritigenin were identified by electrospray ionization-MS (ESI-MS) and NMR analysis.
Ma
Cheng-Jun
CJ
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Li
Gui-sheng
GS
Zhang
Da-lei
DL
Liu
Ke
K
Fan
Xiao
X
D002599:Chalcone / Q000031:analogs & derivatives* / Q000302:isolation & purification; D047188:Chalcones; D003377:Countercurrent Distribution / Q000379:methods*; D044950:Flavanones / Q000302:isolation & purification*; D006035:Glycyrrhiza / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D047188:Chalcones; D044950:Flavanones; D002599:Chalcone; C040920:isoliquiritigenin; C083152:liquiritigenin