Intake of Mediterranean vegetable soup treated by pulsed electric fields affects plasma vitamin C and antioxidant biomarkers in humans.
The bioavailability of vitamin C from pulsed electric fields (PEF)-treated vegetable soup in comparison with freshly made (FM) vegetable soup-gazpacho-and its impact on 8-epiPGF(2alpha) and uric acid concentrations in a human population were assessed. For this purpose six subjects consumed 500 ml PEF-treated vegetable soup/day, and six subjects consumed 500 ml FM vegetable soup/day for 14 days. On the first day of the study, the subjects drank the vegetable soup in one dose (dose-response study), and on days 2-14 they consumed 250 ml in the morning and 250 ml in the afternoon (multiple-dose-response study). Blood was collected every hour for 6 h on the first day and again on days 7 and 14. All blood samples were analyzed for vitamin C, 8-epiPGF(2alpha), and uric acid. The maximum increase in plasma vitamin C occurred 3 h post-dose in both the PEF and the FM groups. Vitamin C remained significantly higher (P = 0.05) on days 7 and 14. The plasma 8-epiPGF(2alpha) concentration was significantly lower at the end of the study in both the PEF group (P = 0.002) and the FM group (P = 0.05). Plasma levels of vitamin C and 8-epiPGF(2alpha) were inversely correlated in both groups (r= -0.549, P = 0.018; and r = -0.743, P = 0.0004, respectively). To summarize, drinking two servings (500 ml) of PEF-treated or FM gazpacho daily increases plasma vitamin C and significantly decreases 8-epiPGF(2alpha) concentrations in healthy humans.
Sánchez-Moreno
Concepción
C
Department of Plant Foods Science and Technology, Instituto del Frío-CSIC, Madrid, Spain. csanchezm@if.csic.es
Pilar Cano
M
M
De Ancos
Begoña
B
Plaza
Lucía
L
Olmedilla
Begoña
B
Granado
Fernando
F
Elez-Martínez
Pedro
P
Martín-Belloso
Olga
O
Martín
Antonio
A
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004435:Eating / Q000502:physiology*; D004560:Electricity; D005260:Female; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D008297:Male; D009753:Nutritive Value; D014527:Uric Acid / Q000097:blood; D014662:Vasoconstrictor Agents / Q000097:blood; D014675:Vegetables
D000975:Antioxidants; D015415:Biomarkers; D014662:Vasoconstrictor Agents; D014527:Uric Acid; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D001205:Ascorbic Acid