Production of scleroglucan from Sclerotium rolfsii MTCC 2156.
Scleroglucan, a neutral homopolysaccaride consisting of a linear chain of beta-D-(1-3)-glucopyranosyl and beta-D-(1-6)-glucopyranosyl groups, was produced by pure culture fermentation from Sclerotium rolfsii MTCC 2156 by submerged culture. Fermentation process was optimized in two steps. In the first step, one-factor-at-a-time method was used to investigate the effects of medium constituents such as carbon and nitrogen sources. In the second step, concentration of medium components was optimized using an L16-orthogonal array method. In all, 10 different carbon sources and eight different nitrogen sources were evaluated. Maximum yield of 16.58 g/l was obtained in a medium containing sucrose as a carbon source and sodium nitrate and yeast extract as nitrogen source.
Survase
Shrikant A
SA
Food and Fermentation Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
Saudagar
Parag S
PS
Singhal
Rekha S
RS
D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D005936:Glucans; D009566:Nitrates; C045938:scleroglucan; D013395:Sucrose; D002244:Carbon; C031618:sodium nitrate; D009584:Nitrogen