Analysis of flavanone-7-O-glycosides in citrus juices by short-end capillary electrochromatography.
The separation of the major flavanone-7-O-glycoside constituents of Citrus was carried out by isocratic reversed phase capillary electrochromatography using a 75 microm i.d. silica fused column packed with 5 microm ODS silica gel. In comparison to HPLC mode, capillary electrochromatography resolution of flavanone glycosides was obtained with a high selectivity factor. Optimum separation conditions were found using a mixture of ammonium formate (pH 2.5)--acetonitrile (8:2, v/v) as the mobile phase by the short-end injection mode. Under these conditions all the investigated flavanones were baseline-resolved within short analysis time (i.e. between 5 and 10 min). A study, evaluating the intra- and inter-day repeatability as well as limit of detection and method linearity, was developed in accordance with the analytical procedures for method validation. The developed method was applied for the quantitative analysis of flavanone glycosides in commercial fruit juices (sweet orange, lemon and grapefruit).
Desiderio
Claudia
C
Istituto di Metodologie Chimiche, CNR, Area della Ricerca di Roma 1, 00016 Monterotondo Stazione, Rome, Italy. claudia.desiderio@imc.cnr.it
De Rossi
Antonella
A
Sinibaldi
Massimo
M
D000097:Acetonitriles; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D044950:Flavanones / Q000032:analysis* / Q000302:isolation & purification; D005561:Formates; D006027:Glycosides / Q000032:analysis*; D006569:Hesperidin / Q000032:analysis / Q000302:isolation & purification; D015203:Reproducibility of Results
D000097:Acetonitriles; D004187:Disaccharides; D044950:Flavanones; D005561:Formates; D006027:Glycosides; C500601:narirutin; C030544:formic acid; C114706:eriocitrin; D006569:Hesperidin; C005274:naringin; C032159:acetonitrile