Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence.
In many model systems, cystic fibrosis (CF) phenotype airway epithelial cells in culture respond to P. aeruginosa with greater interleukin (IL)-8 and IL-6 secretion than matched controls. In order to test whether this excess inflammatory response results from the reported increased adherence of P. aeruginosa to the CF cells, we compared the inflammatory response of matched pairs of CF and non CF airway epithelial cell lines to the binding of GFP-PAO1, a strain of pseudomonas labeled with green fluorescent protein. There was no clear relation between GFP-PAO1 binding and cytokine production in response to PAO1. Treatment with exogenous aGM1 resulted in greater GFP-PAO1 binding to the normal phenotype compared to CF phenotype cells, but cytokine production remained greater from the CF cell lines. When cells were treated with neuraminidase, PAO1 adherence was equalized between CF and nonCF phenotype cell lines, but IL-8 production in response to inflammatory stimuli was still greater in CF phenotype cells. The polarized cell lines 16HBEo-Sense (normal phenotype) and Antisense (CF phenotype) cells were used to test the effect of disrupting tight junctions, which allows access of PAO1 to basolateral binding sites in both cell lines. IL-8 production increased from CF, but not normal, cells. These data indicate that increased bacterial binding to CF phenotype cells cannot by itself account for excess cytokine production in CF airway epithelial cells, encourage investigation of alternative hypotheses, and signal caution for therapeutic strategies proposed for CF that include disruption of tight junctions in the face of pseudomonas infection.
Kube
Dianne M
DM
Department of Pediatrics, Case Western Reserve University School of Medicine, BRB 8th floor, 2109 Adelbert Rd, Cleveland, OH 44106, USA. dmkube@adelphia.net
Fletcher
David
D
Davis
Pamela B
PB
D001422:Bacterial Adhesion / Q000276:immunology; D002460:Cell Line; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology*; D016207:Cytokines / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D020545:Respiratory Mucosa / Q000276:immunology* / Q000382:microbiology*
D016207:Cytokines