title: Polymorphism of the p53 tumor suppressor gene is associated with susceptibility to uterine leiomyoma.
abstract: OBJECTIVE
  To evaluate the association between the presence of uterine leiomyoma and two single nuclear polymorphisms of the p53 tumor suppressor and the angiopoietin-2 (ANGPT2) genes.
  
  
  METHODS
  Prospective case control study.
  
  
  METHODS
  Academic research institution.
  
  
  METHODS
  One hundred thirty-two women with clinically and surgically diagnosed uterine leiomyomas and 280 controls.
  
  
  METHODS
  Peripheral venous puncture.
  
  
  METHODS
  Genotyping was performed by polymerase chain reaction-based amplification of the Arg and Pro variants at codon 72 of the p53 gene and by restriction fragment length polymorphism analysis of the G/G and G/A alleles in exon 4 of the ANGPT2 gene.
  
  
  RESULTS
  Comparing women with uterine leiomyomas and controls, no statistically significant difference with respect to allele frequency and genotype distribution were ascertained for the ANGPT2 polymorphism (P=.2 and P=.5, respectively). However, for the p53 tumor suppressor gene polymorphism, statistically significant differences in terms of a higher Pro allele frequency and a higher prevalence of the Pro/Pro genotype among women with uterine leiomyoma (32.0% vs. 16.0%, respectively, and 21.3% vs. 4.7%, respectively) were ascertained (P=.001, OR 1.74; 95% CI 1.24-2.45, P=.001; OR 3.84, 95% CI 1.81-8.14; respectively).
  
  
  CONCLUSIONS
  Carriage of the p53 polymorphism at codon 72 predicts the susceptibility to leiomyoma in a Caucasian population and may contribute to the pathogenesis of uterine leiomyoma.
authors: Department of Obstetrics and Gynecology, University of Freiburg School of Medicine, Freiburg, Germany. ddenschl@frk.ukl.uni-freiburg.de
authors: Dominik
authors: D
authors: Denschlag
authors: Herta
authors: H
authors: Bettendorf
authors: Dirk
authors: D
authors: Watermann
authors: Christoph
authors: C
authors: Keck
authors: Clemens
authors: C
authors: Tempfer
authors: Detlef
authors: D
authors: Pietrowski
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D016158:Genes, p53 / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53