title: Evaluation of estrogenic and androgenic activity of butylated hydroxyanisole in immature female and castrated rats.
abstract: We evaluated the estrogenic and androgenic activity of butylated hydroxyanisole (BHA) using immature rat uterotrophic assay and Hershberger assay. To investigate (anti-) estrogenicity, BHA alone or with 17beta-estradiol was administered to 20-days-old immature female rats for three consecutive days. Absolute and relative uterine weights were significantly decreased by BHA (50, 100, 250, 500 mg/kg) alone and 17beta-estradiol-stimulated weights of uterine and vagina were also decreased by BHA (500 mg/kg), while uterine epithelial cell height was not affected. In Hershberger assay, BHA alone or with testosterone propionate (TP) was administered daily to 51-days-old castrated male rats for 10 days. BHA alone or with testosterone propionate (TP) caused no significant effect on androgen-dependent accessory sex organ weights; seminal vesicle/coagulative glands, glans penis, Cowper's gland, ventral prostate gland and levator ani plus bulbocarvernosus muscle. Although, the relative weight of ventral prostate gland was increased by the co-treatment of BHA 250 mg/kg with TP 0.4 mg/kg compared to that of TP alone, the relative and absolute weights of other androgen-dependent organs and absolute and formalin-fixed ventral prostate gland weight showed no changes. Our studies suggest that BHA have anti-estrogenic activity for the decrease of uterine weight in immature female rat but have negligible effect on the androgenic activity in castrated male rats.
authors: Toxicology Lab, Division of Toxicology, National Veterinary Research and Quarantine Service, 480 Anyang 6-Dong, Anyang 430-824, Republic of Korea.
authors: Hwan Goo
authors: HG
authors: Kang
authors: Sang Hee
authors: SH
authors: Jeong
authors: Joon Hyoung
authors: JH
authors: Cho
authors: Dong Gyu
authors: DG
authors: Kim
authors: Jong Myung
authors: JM
authors: Park
authors: Myung Haing
authors: MH
authors: Cho
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D008297:Male; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D043343:Testosterone Propionate / Q000097:blood / Q000378:metabolism; D013974:Thyroxine / Q000097:blood
chemical_list: D000975:Antioxidants; D002083:Butylated Hydroxyanisole; D004958:Estradiol; D013974:Thyroxine; D043343:Testosterone Propionate