Expression of multiple drug resistance conferring proteins in normal Chinese and Caucasian small and large intestinal tissue samples.
Multidrug resistance conferring proteins (MDRCP) are ATP-binding cassette (ABC) transporters known to significantly influence the absorption, distribution, metabolism, and elimination (ADME) and toxic behavior of many therapeutic agents. Research in the pharmacogenomics area has suggested that mutations and variable expression patterns of these MDCRPs may exist in tissue samples from different ethnic groups. The goal of this study was to examine the expression of P-glycoprotein (PGP), sister of PGP (S-PGP), multidrug resistance protein 3 (Mdr3), multidrug resistance like proteins 1-5 (MRP 1-5), and lung resistance associated protein (LRP) in tissue slides and protein lysates derived from normal adult small or large intestines of Caucasian or Chinese origin. Our results demonstrated ubiquitous expression of PGP, MRP 1, MRP 4, and LRP in the small and large intestinal epitheliums originating from both Caucasian and Chinese origin. S-PGP, Mdr3, MRP 2, and MRP 3 exhibited variable expression in the tissue slides and protein lysates derived from the Chinese and Caucasian small and large intestines. MRP 5 was not observed in any of the samples studied. The results suggest that MDCRPs may have distinct expression profiles in the small and large intestines that potentially vary with genetic background. These studies provide a foundation for further investigations to verify these findings across a wider number of patients of different ethnic backgrounds.
Wang
Qing
Q
Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854-8022, USA.
Bhardwaj
Rajinder K
RK
Herrera-Ruiz
Dea
D
Hanna
Nazeeh N
NN
Hanna
Iman T
IT
Gudmundsson
Olafur S
OS
Buranachokpaisan
Thitiwan
T
Hidalgo
Ismael J
IJ
Knipp
Gregory T
GT
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis / Q000096:biosynthesis*; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000096:biosynthesis*; D000328:Adult; D044466:Asian People; D002681:China; D018432:Drug Resistance, Multiple; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis / Q000096:biosynthesis*; D012016:Reference Values; D044465:White People
D018435:ATP Binding Cassette Transporter, Subfamily B; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C093491:ABCB11 protein, human; C000715087:ABCC2 protein, human; C095122:multidrug resistance protein 3