Enzymes that hydrolyze adenine nucleotides in chronic renal failure: relationship between hemostatic defects and renal failure severity.
The activities of the enzymes NTPDase (E.C.3.6.1.5, apyrase, ATP diphosphohydrolase, ecto-CD 39) and 5'-nucleotidase (E.C.3.1.3.5, CD 73) were analyzed in platelets from patients with chronic renal failure (CRF), both undergoing hemodialysis treatment (HD) and not undergoing hemodialysis (ND), as well as from a control group. The results showed an increase in platelet NTPDase activity in CRF patients on HD treatment (52.88%) with ATP as substrate (P<0.0001). ADP hydrolysis was decreased (33.68% and 39.75%) in HD and ND patients, respectively. In addition, 5'-nucleotidase activity was elevated in the HD (160%) and ND (81.49%) groups when compared to the control (P<0.0001). Significant correlation was found among ATP, ADP and AMP hydrolysis and plasma creatinine and urea levels (P<0.0001). Patients were compared statistically according the time of hemodialysis treatment. We found enhanced NTPDase and 5'-nucleotidase activities between 49 and 72 months on HD patients. Our result suggests the existence of alterations in nucleotide hydrolysis in platelets of CRF patients. Possibly, this altered nucleotide hydrolysis could contribute to hemostasis abnormalities found in CRF.
Silva
Adriane C
AC
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Avenue Roraima, 97105-900, Santa Maria, RS, Brazil.
Morsch
André L B
AL
Zanin
Rafael F
RF
Corrêa
Maísa C
MC
Arantes
Luís C
LC
Araujo
Maria C
MC
Morsch
Vera M
VM
Schetinger
Maria R C
MR
D015720:5'-Nucleotidase / Q000378:metabolism*; D000225:Adenine / Q000378:metabolism*; D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D003401:Creatine / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000628:therapy; D006435:Renal Dialysis; D013997:Time Factors; D014508:Urea / Q000097:blood
D015703:Antigens, CD; D014508:Urea; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen; D000225:Adenine; D003401:Creatine