title: Motilin inhibits ganglionic transmission in the myenteric plexus of the guinea-pig ileum.
abstract: Motilin is a key factor in triggering interdigestive migrating contractions. Our preceding study demonstrated that motilin caused membrane depolarizations in a minority of S and AH neurons in the myenteric plexus of the guinea-pig ileum after 18 h-fasting period; motilin depolarizations were small and seldom triggered action potentials. Then, the present study was undertaken to examine possible electrophysiological actions of motilin on the ganglionic transmission in the myenteric plexus. Intracellular recordings with sharp glass microelectrodes were made from myenteric S neurons having fast excitatory postsynaptic potentials (EPSPs), evoked by focal electrical stimulation. Motilin inhibited the fast EPSPs in amplitude, associated either with or without membrane depolarizations. Results obtained with the paired stimulus method suggested that the site for motilin-induced inhibition of fast EPSPs might be presynaptic. Furthermore, motilin did not decrease postsynaptic sensitivity to ACh, a main neurotransmitter mediating the fast EPSPs. Therefore, it is concluded that motilin might inhibit presynaptically ganglionic transmission in the myenteric plexus of the guinea-pig ileum.
authors: Department of Autonomic Physiology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan. kataauto@tmd.ac.jp
authors: Yoshifumi
authors: Y
authors: Katayama
authors: Yumi
authors: Y
authors: Noda
authors: Keiji
authors: K
authors: Hirai
authors: Kazuki
authors: K
authors: Honda
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D005725:Ganglia, Autonomic / Q000187:drug effects* / Q000502:physiology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008839:Microelectrodes; D009037:Motilin / Q000494:pharmacology*; D009197:Myenteric Plexus / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology
chemical_list: D009037:Motilin