The effect of radio-frequency glow discharge treatment of polystyrene on the behavior of porcine chondrocytes in vitro.
The aim of this study was to determine the effects of physicochemical surface properties of tissue-culture substrata on chondrocyte behavior. Polystyrene was modified by radio-frequency glow discharge (RFGD) plasma treatment with various monomers. The changes in surface properties of the modified polystyrene were verified by ESCA and water contact angle measurements. Porcine chondrocytes were seeded on these surfaces and cultured for 5 days. After 5 days of culture, the number of chondrocytes was highest on the N2 plasma-treated surface, followed by the CH2/N2 plasma-treated surface, untreated polystyrene and CF4 plasma-treated surface. The number of chondrocytes decreased with increasing water contact angle. The surface chemical properties influenced the morphology and gene expression of cultured chondrocytes. The cells cultured on the CF4 plasma-treated surface retained a round morphology characteristic of chondrocytes after day 1, while most of the cells grown on the N2 plasma-treated surface or the untreated polystyrene showed a flattened morphology. Using RT-PCR, expression of type-I collagen could not be detected in the chondrocytes cultured on the CF4 plasma-treated surface and the CH2/N2 plasma-treated surface. In contrast, the chondrocytes grown on the N2 plasma-treated surface or the untreated polystyrene surface expressed type-I collagen mRNA. This study shows that modification by RFGD treatment could modulate chondrocyte culture and gene expression.
Tsai
Wei-Bor
WB
Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Road, Sec. 4, Taipei 106, Taiwan. weibortsai@coms.ntu.edu.tw
Wei
Ta-Chin
TC
Lin
Mei-Chiao
MC
Wang
Jie-Ying
JY
Chen
Chun-Hong
CH
D000114:Acetylene; D000818:Animals; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics; D005466:Fluorocarbons; D005786:Gene Expression Regulation / Q000187:drug effects; D009584:Nitrogen; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology*; D011846:Radio Waves; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D005466:Fluorocarbons; D011137:Polystyrenes; C035066:carbon tetrafluoride; D009584:Nitrogen; D000114:Acetylene