title: Anopheles infection responses; laboratory models versus field malaria transmission systems.
abstract: The molecular biology of disease vectors, particularly mosquitoes, has experienced a remarkable progress in the past two decades. This is mainly attributed to methodological advances and the emerging genome sequences of vector species, which have brought experimental biology to an unprecedented level. It is now possible to determine the entire transcriptome of Anopheles gambiae at a variety of conditions, with a low per-gene effort and cost. Proteomic profiles can be generated for as small samples as the hemolymph, and transient reverse genetic and stable germ line based transgenic analyses can be performed to analyze gene function. High throughput screening for receptors and ligands can be used to characterize interactions between vectors and pathogens. At the current breathtaking rates of data production it is essential to question and evaluate the relevance of laboratory infection models to the real disease transmission systems. The majority of scientific discoveries in mosquito molecular biology have been based on highly inbred laboratory strains and rodent malaria parasite infection models, which may differ substantially to their counterparts that transmit human malaria in the field. This review addresses the recent advances in high throughput transcription analyses of Anopheles responses to infection, and discusses considerations for the use of laboratory malaria infection models.
authors: W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205-2179, USA.
authors: Ruth
authors: R
authors: Aguilar
authors: Yuemei
authors: Y
authors: Dong
authors: Emma
authors: E
authors: Warr
authors: George
authors: G
authors: Dimopoulos
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D019411:Clinical Laboratory Techniques / Q000639:trends*; D004199:Disease Vectors; D006801:Humans; D008288:Malaria / Q000517:prevention & control / Q000635:transmission*; D008967:Molecular Biology / Q000639:trends*; D010961:Plasmodium / Q000276:immunology / Q000502:physiology*