title: [The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line].
abstract: OBJECTIVE
  To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.
  
  
  METHODS
  Cell proliferation was assayed by MTT analysis and [3H] thymidine incorporation, after Hep-2 cell line culturing with M-CSF and M-CSFR. M-CSF concentration of surpernatant measured by a ELISA technique. Immunohistochemistry was performed on Hep-2 cell line and laryngeal carcinoma tissue.
  
  
  RESULTS
  The concentration of M-CSF increased after cell culturing. M-CSF can reduced the Hep-2 cell count. The action can be blocked by Anti-M-CSFR.
  
  
  CONCLUSIONS
  Our observations support the hypothesis that M-CSF may inhibit the growth and differentiation in Hep-2 cell line.
authors: Department of Otolaryngology-Head and Neck Surgery, Guangdong Province People's Hospital, Guangzhou, 510080, China.
authors: Pingjiang
authors: P
authors: Ge
authors: Baoquan
authors: B
authors: Zhang
authors: Jianmin
authors: J
authors: Zhang
authors: Zhiqiang
authors: Z
authors: Gao
authors: Qingli
authors: Q
authors: Kong
authors: Wei
authors: W
authors: He
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000494:pharmacology*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism
chemical_list: D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor