BFP protein expression in transfected embryonic stem cells.
Cultured mouse embryonic stem cells can be transfected with a reporter gene encoding blue fluorescent protein BFP and regulated by drosophila heat shock protein 70 promoter. This gene is activated after heating and synthesizes matrix RNA. Blue protein is synthesized under these conditions. The system for transfection of stem cells allows us to activate automatically the corresponding transgenes.
Pavlova
G V
GV
Institute of Gene Biology, Russian Academy of Sciences, Institute of Molecular Genetics, Russian Academy of Sciences, Russia.
Manuilova
E S
ES
Arsen'eva
E L
EL
Grivennikov
I A
IA
Murkin
E V
EV
Kanaikina
N V
NV
Revishchin
A V
AV
Tarantul
V N
VN
Korochkin
L I
LI
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D011506:Proteins / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection
D011506:Proteins