title: Distribution of leucocyte subsets in bronchial mucosa from dogs with eosinophilic bronchopneumopathy.
abstract: Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from all dogs were included in the study, but frozen tissue from only one dog was available. MHC class II(+) cells were found in moderate numbers in the lamina propria (LP). These cells were morphologically either dendritic-like cells or macrophages, but many macrophages did not express MHC class II. Such molecules were expressed by occasional fibroblasts. L1(+) cells, which formed a relatively small component of the LP inflammatory infiltrate, were morphologically either macrophages or polymorphonuclear cells (probably eosinophils). IgA(+) plasma cells were found in varying numbers in the LP, mostly in association with glandular tissue. IgG(+) plasma cells were less common, and IgM(+) plasma cells were present in low numbers. Many CD3(+) cells were present in the LP. In the single case from which frozen tissue was available, most of the lymphocytes were labelled with CD4 marker, while smaller numbers were CD8(+) T cells. Most of the lymphocytes in this case were positively labelled with T-cell receptor (TCR)-alphabeta marker. TCR-gammadelta(+) cells, although less common, were present in significant numbers throughout the LP. CDlc(+) dendritic cells were numerous in the epithelium and in the LP, immediately beneath the basement membrane. These findings, which were similar to those described in human asthma, are suggestive of a Th2 dominant immune response in canine EBP. As in human asthma, this provides a possible basis for new forms of treatment in canine EBP.
authors: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors: D
authors: D
authors: Peeters
authors: M J
authors: MJ
authors: Day
authors: C
authors: C
authors: Clercx
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D015415:Biomarkers / Q000378:metabolism; D001980:Bronchi / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007962:Leukocytes / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D011657:Pulmonary Eosinophilia / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D000949:Histocompatibility Antigens Class II