Movement suppression during anesthesia: neural projections from the mesopontine tegmentum to areas involved in motor control.
Microinjection of pentobarbital and GABA(A)-receptor agonists into a brainstem region we have called the mesopontine tegmental anesthesia area (MPTA; Devor and Zalkind [2001] Pain 94:101-112) induces a general anesthesia-like state. As in systemic general anesthesia, rats show loss of the righting reflex, atonia, nonresponsiveness to noxious stimuli, and apparent loss of consciousness. GABA(A) agonist anesthetics acting on the MPTA might suppress movement by engaging endogenous motor regulatory systems previously identified in research on decerebrate rigidity and REM sleep atonia. Anterograde and retrograde tracing revealed that the MPTA has multiple descending projections to pontine and medullary areas known to be associated with motor control and atonia. Prominent among these are the dorsal pontine reticular formation and components of the rostral ventromedial medulla (RVM). The MPTA also has direct projections to the intermediate gray matter and ventral horn of the spinal cord via the lateral and anterior funiculi. These projections show a rostrocaudal topography: neurons in the rostral MPTA project to the RVM, but only minimally to the spinal cord, while those in the caudal MPTA project to both targets. Finally, the MPTA has ascending projections to motor control areas including the substantia nigra, subthalamic nucleus, and the caudate-putamen. Projections are bilateral with an ipsilateral predominance. We propose that GABA(A) agonist anesthetics induce immobility at least in part by acting on these endogenous motor control pathways via the MPTA. Analysis of MPTA connectivity has the potential for furthering our understanding of the neural circuitry responsible for the various functional components of general anesthesia.
Sukhotinsky
Inna
I
Department of Cell and Animal Biology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Hopkins
David A
DA
Lu
Jun
J
Saper
Clifford B
CB
Devor
Marshall
M
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001479:Basal Ganglia / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives; D002772:Cholera Toxin; D003243:Consciousness / Q000187:drug effects / Q000502:physiology; D003911:Dextrans; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D009068:Movement / Q000187:drug effects / Q000502:physiology*; D009129:Muscle Tonus / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009434:Neural Pathways / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D011149:Pons / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012154:Reticular Formation / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D013681:Tegmentum Mesencephali / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*
D000777:Anesthetics; D003911:Dextrans; C076397:biotinylated dextran amine; D001710:Biotin; D002772:Cholera Toxin