title: Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products.
abstract: A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay.
authors: Department of Biomedical Sciences and Technologies, University of Milan, Via Fratelli Cervi 93, Segrate, Milan, Italy.
authors: Paola
authors: P
authors: Cremonesi
authors: Massimo
authors: M
authors: Luzzana
authors: Milena
authors: M
authors: Brasca
authors: Stefano
authors: S
authors: Morandi
authors: Roberta
authors: R
authors: Lodi
authors: Chiara
authors: C
authors: Vimercati
authors: Dario
authors: D
authors: Agnellini
authors: Giancarlo
authors: G
authors: Caramenti
authors: Paolo
authors: P
authors: Moroni
authors: Bianca
authors: B
authors: Castiglioni
mesh_terms: D000818:Animals; D003611:Dairy Products / Q000382:microbiology*; D004768:Enterotoxins; D005506:Food Contamination; D006801:Humans; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics*
chemical_list: D004768:Enterotoxins; D012338:RNA, Ribosomal, 23S