Nucleotide sequence and genomic organization of a newly isolated densovirus infecting Dendrolimus punctatus.
The nucleotide sequence of a novel icosahedral DNA virus infecting Dendrolimus punctatus has been determined. The genome is 5039 nt long and includes inverted terminal repeats of 200 nt containing 131 nt long J-shaped terminal hairpins. The 'plus' strand of the genome contains three large open reading frames (ORFs), the left and the mid-ORFs (within the left ORF) in the left-half encoding the non-structural proteins and the right ORF in the right-half encoding viral capsid proteins. NS1 protein contains conserved replication initiation and DNA-dependent ATPase/helicase domains. VP1 protein contains a conserved PGY and phospholipase A2 motifs and shows high identities with VPs of Casphalia extranea densovirus and Bombyx mori densovirus-1 belonging to the genus Iteravirus. Phylogenetic analysis also revealed that this virus is most closely related to Casphalia extranea densovirus and Bombyx mori densovirus-1. Consequently, this virus was considered as a new third member of the genus Iteravirus of the subfamily Densovirinae, and designated Dendrolimus punctatus densovirus.
Wang
Junping
J
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Zhang
Jiamin
J
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Jiang
Hong
H
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Liu
Chuanfeng
C
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Yi
Fuming
F
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Hu
Yuanyang
Y
Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics; D017990:Densovirus / Q000235:genetics*; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010741:Phospholipases A / Q000235:genetics; D054467:Phospholipases A2; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D017361:Viral Nonstructural Proteins; C045264:replication initiator protein; D010741:Phospholipases A; D054467:Phospholipases A2; D004265:DNA Helicases