title: Characterization of prolidase activity in erythrocytes from a patient with prolidase deficiency: comparison with prolidase I and II purified from normal human erythrocytes.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of various amino acids and their metabolites on the activities of prolidase I and II from human erythrocytes compared to those in a patient with prolidase deficiency.
  
  
  METHODS
  Prolidase I and II from human erythrocytes were purified by using column chromatography. Prolidase activity against various iminodipeptides was determined by spectrophotometry using Chinard's method.
  
  
  RESULTS
  The activities of prolidase I and II against glycylproline and methionylproline were enhanced by glycine, L- and D-isoforms of alanine and serine and D-isoforms of valine, leucine and isoleucine. L-isoforms of branched amino acids inhibited the activity of prolidase I. On the other hand, the activity of prolidase II was enhanced by all of these L-branched amino acids. The patient's prolidase activity was also enhanced by all the L- and D-branched amino acids.
  
  
  CONCLUSIONS
  The activities of prolidase I and II against various iminodipeptides were prominently enhanced by glycine, but the effect of L-valine differed between the two enzymes. Enzymatic properties of the patient's prolidase were essentially the same as those of prolidase II.
authors: Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi 783-8505, Japan.
authors: Gang
authors: G
authors: Liu
authors: Kazuko
authors: K
authors: Nakayama
authors: Yasuhiro
authors: Y
authors: Sagara
authors: Shiro
authors: S
authors: Awata
authors: Koichi
authors: K
authors: Yamashita
authors: Masanobu
authors: M
authors: Manabe
authors: Hiroyuki
authors: H
authors: Kodama
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004150:Dipeptidases / Q000097:blood* / Q000172:deficiency* / Q000302:isolation & purification; D004151:Dipeptides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D017895:Manganese Compounds / Q000494:pharmacology; D013237:Stereoisomerism; D014633:Valine / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D002712:Chlorides; D004151:Dipeptides; D017895:Manganese Compounds; C015248:glycylproline; D004150:Dipeptidases; C022449:proline dipeptidase; D014633:Valine; C025340:manganese chloride