title: [Cold stress causes early apoptosis in caudal fin cells of Colossoma brachypomum].
abstract: CBT cell line developed from a warm-water fish Colossoma Brachypomum,was used to evaluate the effects of cold stress on fish cells. Cell viability was measured by Thiazolyl blue (MTT) method and cellular ultrastructure was examined under transmission electron microscope. Externalization of phosphatidylserine(PS) and mitochondrial membrane potential(MMP) were monitored with confocal laser scanning microscope. Results showed that cell viability and MMP decreased in a time-dependent manner, and the early events of apoptosis such as chromatin condensation, margination and PS externalization occurred, when the cells were exposed to 10 degrees C. It suggested that low temperature (10 degrees C) could induce apoptosis in Colossoma Brachypomum CBT cells.
authors: College of Life Sciences, Zhejiang University, Hangzhou 310012.
authors: Wen Jing
authors: WJ
authors: Yan
authors: Han Min
authors: HM
authors: Chen
authors: Xiao Li
authors: XL
authors: Zhao
authors: Hong
authors: H
authors: Yu
authors: Ming
authors: M
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D003080:Cold Temperature; D005399:Fishes; D053078:Membrane Potential, Mitochondrial / Q000502:physiology