Expression of the promoter of HyPRP, an embryo-specific gene from Zea mays in maize and tobacco transgenic plants.
zmHyPRP is a gene specifically expressed in maize immature embryos where its transcripts are mainly observed in the scutellum. It has been shown that zmHyPRP expression in the embryo is arrested when ABA levels increase at the beginning of the maturation stage. Here we report the ability of 2 Kb zmHyPRP promoter to reproduce the zmHyPRP gene specific expression pattern in the maize embryo and its repression by ABA at the end of the morphogenetic process. Three different approaches have been used, transient particle bombardment of maize immature excised embryos and stable transformation of maize and tobacco plants with a construct containing 2 Kb of zmHyPRP promoter fused to the GUS gene. This construct has shown to confer specific expression to maize and tobacco embryos but in tobacco expression in the embryo was very low. The same construct was also negatively regulated by ABA in embryos of both species. This suggests that 2 Kb of the zmHyPRP promoter contain all regulatory elements sufficient to confer the developmental expression patterns of the gene characterized to date.
José-Estanyol
Matilde
M
Laboratori Genètica Molecular Vegetal, CSIC-IRTA, IBMB-CSIC, c/. Jordi Girona, 18, 08034-Barcelona, Spain. mjegmp@ibmb.csic.es
Pérez
Pascual
P
Puigdomènech
Pere
P
D000040:Abscisic Acid / Q000494:pharmacology; D005470:Fluorometry / Q000379:methods; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D003313:Zea mays / Q000187:drug effects / Q000196:embryology / Q000235:genetics*
D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C076045:hybrid proline-rich protein, plant; D000040:Abscisic Acid; D005966:Glucuronidase