[Effect of Xingding Injection on expression of heat shock protein 72 in vascular endothelial cells].
OBJECTIVE
To study the effect of Xingding Injection on the expression of heat shock protein 72 (HSP 72) in vascular endothelial cells after ultraviolet radiation.


METHODS
Porcine aortic endothelial cells were cultured for 72 hours in culture mediums with different concentrations (0.1, 0.5, 1.0, 5.0, 10 mg/ml) of Xingding Injection. Ultraviolet radiation was administered to the cultured cells for 30 minutes. Western-blot assay was used to measure the expression of HSP 72 in the vascular endothelial cells.


RESULTS
There was no expression of HSP 72 in the cultured vascular endothelial cells without ultraviolet radiation, but there was some expression of HSP 72 after ultraviolet radiation. Xingding Injection of different concentrations could significantly improve the expression of HSP 72. The expression of HSP 72 in the vascular endothelial cells cultured in culture medium with 1.0 mg/ml Xingding Injection was the highest, and there was no more increase of expression when the concentration was higher, instead the expression decreased.


CONCLUSIONS
Xingding Injection can protect the vascular endothelial cells from injury during stress. It may be one of its mechanisms in preventing and treating cardio-cerebrovascular disorders.
Li
Jing
J
Department of Emergency, Hubei General Hospital of Chinese People's Armed Police Forces, Wuhan, Hubei Province 430060, China.
Wu
Yun-Xia
YX
Zhang
Mei-Chun
MC
D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D011827:Radiation; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013552:Swine; D014466:Ultraviolet Rays
D004365:Drugs, Chinese Herbal; D050884:HSP72 Heat-Shock Proteins; D011837:Radiation-Protective Agents