title: Clear evidence of fluorescence resonance energy transfer in gas-phase ions.
abstract: Fluorescence resonance energy transfer (FRET) is a distance-sensitive method that correlates changes in fluorescence intensity with conformational changes, for example, of biomolecules in the cellular environment. Applied to the gas phase in combination with Fourier transform ion cyclotron resonance mass spectrometry, it opens up possibilities to define structural/conformational properties of molecular ions, in the absence of solvent, and without the need for purification of the sample. For successfully observing FRET in the gas phase it is important to find suitable fluorophores. In this study several fluorescent dyes were examined, and the correlation between solution-phase and gas-phase fluorescence data were studied. For the first time, FRET in the gas phase is demonstrated unambiguously.
authors: Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland.
authors: Maxim
authors: M
authors: Dashtiev
authors: Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland.
authors: Vladimir
authors: V
authors: Azov
authors: Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland.
authors: Vladimir
authors: V
authors: Frankevich
authors: Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland.
authors: Ludwig
authors: L
authors: Scharfenberg
authors: Department of Chemistry, ETH Honggerberg, Swiss Federal Institute of Technology, HCI E 329, CH-8093, Zurich, Switzerland. zenobi@org.chem.ethz.ch.
authors: Renato
authors: R
authors: Zenobi
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005740:Gases / Q000737:chemistry*; D007477:Ions; D044367:Phase Transition; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D005740:Gases; D007477:Ions