High throughput assay for the determination of lumefantrine in plasma.
A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1% at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling.
Annerberg
A
A
Wellcome unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Singtoroj
T
T
Tipmanee
P
P
White
N J
NJ
Day
N P J
NP
Lindegårdh
N
N
D000962:Antimalarials / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D004983:Ethanolamines / Q000097:blood*; D005449:Fluorenes / Q000097:blood*; D006801:Humans; D000078102:Lumefantrine; D015203:Reproducibility of Results
D000962:Antimalarials; D004983:Ethanolamines; D005449:Fluorenes; D000078102:Lumefantrine