title: Shutoff of RNA polymerase II transcription by poliovirus involves 3C protease-mediated cleavage of the TATA-binding protein at an alternative site: incomplete shutoff of transcription interferes with efficient viral replication.
abstract: The TATA-binding protein (TBP) plays a crucial role in cellular transcription catalyzed by all three DNA-dependent RNA polymerases. Previous studies have shown that TBP is targeted by the poliovirus (PV)-encoded protease 3C(pro) to bring about shutoff of cellular RNA polymerase II-mediated transcription in PV-infected cells. The processing of the majority of viral precursor proteins by 3C(pro) involves cleavages at glutamine-glycine (Q-G) sites. We present evidence that suggests that the transcriptional inactivation of TBP by 3C(pro) involves cleavage at the glutamine 104-serine 105 (Q104-S105) site of TBP and not at the Q18-G19 site as previously thought. The TBP Q104-S105 cleavage by 3C(pro) is greatly influenced by the presence of an aliphatic amino acid at the P4 position, a hallmark of 3C(pro)-mediated proteolysis. To examine the importance of host cell transcription shutoff in the PV life cycle, stable HeLa cell lines were created that express recombinant TBP resistant to cleavage by the viral proteases, called GG rTBP. Transcription shutoff was significantly impaired and delayed in GG rTBP cells upon infection with poliovirus compared with the cells that express wild-type recombinant TBP (wt rTBP). Infection of GG rTBP cells with poliovirus resulted in small plaques, significantly reduced viral RNA synthesis, and lower viral yields compared to the wt rTBP cell line. These results suggest that a defect in transcription shutoff can lead to inefficient replication of poliovirus in cultured cells.
authors: Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, University of California--Los Angeles, 90095, USA.
authors: Pallob
authors: P
authors: Kundu
authors: Santanu
authors: S
authors: Raychaudhuri
authors: Weimin
authors: W
authors: Tsai
authors: Asim
authors: A
authors: Dasgupta
mesh_terms: D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D005973:Glutamine; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D012694:Serine; D035181:TATA-Box Binding Protein / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D011506:Proteins; D035181:TATA-Box Binding Protein; D014764:Viral Proteins; D005973:Glutamine; D012694:Serine; D012319:RNA Polymerase II; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases