title: Pregnancies and births resulting from in vitro matured oocytes fertilized with testicular spermatozoa.
abstract: OBJECTIVE
  In vitro maturation (IVM) of immature human oocytes is an attractive option for the treatment of infertility. Similarly, intracytoplasmic sperm injection (ICSI) followed by testicular fine needle aspiration (TEFNA) is an important treatment for primarily male-factor infertility. This report highlights the combination of these two advanced assisted reproduction techniques, namely IVM and fertilization with TEFNA-retrieved spermatozoa by ICSI to overcome both of male and female infertility problems.
  
  
  METHODS
  Before immature oocyte retrieval (IOR), gonadotropin stimulation was given for 3 or 5 days. Following IVM, and mature oocytes were inseminated by ICSI followed by TEFNA.
  
  
  RESULTS
  Four couples with five completed treatment cycles were performed, and total of 36 immature oocytes were retrieved. Following 36 to 48 h of culture, 32 (88.89%, 32/36) oocytes became mature. The mature oocytes were inseminated with TEFNA-retrieved sperm, and 18 (56.25%, 18/32) oocytes were fertilized normally following ICSI. Eleven embryos were transferred in five cycles and two pregnancies and two singleton births were achieved in two patients.
  
  
  CONCLUSIONS
  This result demonstrates that the successful pregnancies and live births can be established from embryos produced from in vitro matured oocytes that fertilized with testicular sperm.
authors: Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, P R China. qianyun@njmu.edu.cn
authors: Qian
authors: Q
authors: Yun
authors: Feng
authors: F
authors: Ting
authors: Juan
authors: J
authors: Chen
authors: Ling-Bo
authors: LB
authors: Cai
authors: Jia-Yin
authors: JY
authors: Liu
authors: Yun-Dong
authors: YD
authors: Mao
authors: Wei
authors: W
authors: Ding
authors: Jia-Hao
authors: JH
authors: Sha
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D006062:Gonadotropins / Q000502:physiology; D006801:Humans; D008297:Male; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis
chemical_list: D006062:Gonadotropins