Ceramides, sphinganine, sphingosine and acid sphingomyelinases in the human umbilical cord blood.
Ceramides, sphingosine, sphinganine, as well as Zn (++)-dependent and Zn (++)-independent acid sphingomyelinase are present in the plasma of adults. The aim of the present study was to examine the concentrations of these compounds and activities of both enzymes in the umbilical cord blood in humans. Twenty-two women with uncomplicated term pregnancy volunteered for the study. Blood was taken from the umbilical cord artery and from the antecubital vein of the mother immediately after delivery. Free ceramides were isolated by thin layer chromatography, and their fatty acids were identified and quantified by gas-liquid chromatography. Free sphingosine and sphinganine concentrations were determined using high-performance liquid chromatography. Acid Zn (++)-dependent and Zn (++)-independent sphingomyelinase activity was measured using sphingomyelin [choline-methyl-14C] as a substrate. We found that the compounds examined are present in the umbilical cord blood. The total fatty acid-containing ceramide concentrations in fetal blood were lower than in mother's blood. The mean sphingosine and sphinganine concentrations in the fetal and maternal serum were similar. The examined enzymes were present in the fetal serum, and their mean activity did not differ from that in the mother. In conclusion, we have shown the presence of ceramides, sphingosine and sphinganine and both isoforms of acid sphingomyelinase in the human fetal cord blood. They are most likely the product of the fetus itself.
Knapp
P
P
Department of Gynecology, Medical University of Białystok, 15-089 Białystok, Poland.
Dobrzyń
A
A
Górski
J
J
D000328:Adult; D002518:Ceramides / Q000097:blood*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D005312:Fetal Blood / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D008055:Lipids / Q000097:blood; D011247:Pregnancy; D013108:Sphingomyelin Phosphodiesterase / Q000097:blood*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood*; D015032:Zinc / Q000502:physiology
D002518:Ceramides; D005227:Fatty Acids; D008055:Lipids; D013108:Sphingomyelin Phosphodiesterase; D015032:Zinc; D013110:Sphingosine; C005682:safingol