title: Cloning and functional analysis of the orotidine-5'-phosphate decarboxylase gene (PbrURA3) of the pathogenic fungus Paracoccidioides brasiliensis.
abstract: A genomic clone encoding the Paracoccidioides brasiliensis orotidine monophosphate decarboxylase gene (PbrURA3) was isolated by screening a subgenomic plasmid DNA library of this fungus, using a PCR amplification product of the gene as a probe. Sequence analysis revealed that the gene contains an open reading frame of 855 bp with a single intron (162 bp), and encodes a putative 285 amino acids polypeptide of estimated molecular weight 31.1 kDa and isoelectric point 6.5. The deduced amino acid sequence predicted a 73.4% identity with orotidine monophosphate decarboxylase of Aspergillus nidulans. Functionality of the gene was demonstrated by transformation into a Saccharomyces cerevisiae ura3 null mutant.
authors: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors: Cristina
authors: C
authors: Reinoso
authors: Françoise
authors: F
authors: Sorais
authors: Gustavo A
authors: GA
authors: Niño-Vega
authors: Encarnación
authors: E
authors: Fermiñán
authors: Gioconda
authors: G
authors: San-Blas
authors: Angel
authors: A
authors: Domínguez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015723:Gene Library; D005816:Genetic Complementation Test; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D010228:Paracoccidioides / Q000201:enzymology* / Q000235:genetics; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014170:Transformation, Genetic
chemical_list: D012331:RNA, Fungal; D009964:Orotidine-5'-Phosphate Decarboxylase