Magneto-optical tweezers built around an inverted microscope.
We present a simple experimental setup of magneto-optical tweezers built around an inverted microscope. Two pairs of coils placed around the focal point of the objective generate a planar-rotating magnetic field that is perpendicular to the stretching direction. This configuration allows us to control the rotary movement of a paramagnetic bead trapped in the optical tweezers. The mechanical design is universal and can be simply adapted to any inverted microscope and objective. The mechanical configuration permits the use of a rather large experimental cell and the simple assembly and disassembly of the magnetic attachment.
Claudet
Cyril
C
Laboratoire de Spectrométrie Physique, Unité Mixte de Recherche 5588, 140 avenue de la Physique, 38402 St. Martin d'Hères, France.
Bednar
Jan
J
D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D008280:Magnetics / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D009690:Nucleic Acid Conformation; D013314:Stress, Mechanical
D004247:DNA