Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation.
Glutaminase from Stenotrophomonas maltophilia NYW-81 was purified to homogeneity with a final specific activity of 325 U/mg. The molecular mass of the native enzyme was estimated to be 41 kDa by gel filtration. A subunit molecular mass of 36 kDa was measured with SDS-PAGE, thus indicating that the native enzyme is a monomer. The N-terminal amino acid sequence of the enzyme was determined to be KEAETQQKLANVVILATGGTIA. Besides L: -glutamine, which was hydrolyzed with the highest specific activity (100%), L: -asparagine (74%), D: -glutamine (75%), and D: -asparagine (67%) were also hydrolyzed. The pH and temperature optima were 9.0 and approximately 60 degrees C, respectively. The enzyme was most stable at pH 8.0 and was highly stable (relative activities from 60 to 80%) over a wide pH range (5.0-10.0). About 70 and 50% of enzyme activity was retained even after treatment at 60 and 70 degrees C, respectively, for 10 min. The enzyme showed high activity (86% of the original activity) in the presence of 16% NaCl. These results indicate that this enzyme has a higher salt tolerance and thermal stability than bacterial glutaminases that have been reported so far. In a model reaction of Japanese soy sauce fermentation, glutaminase from S. maltophilia exhibited high ability in the production of glutamic acid compared with glutaminases from Aspergillus oryzae, Escherichia coli, Pseudomonas citronellolis, and Micrococcus luteus, indicating that this enzyme is suitable for application in Japanese soy sauce fermentation.
Wakayama
Mamoru
M
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi, Kusatsu, 525-8577, Japan. wakayama@se.ritsumei.ac.jp
Yamagata
Tomohiro
T
Kamemura
Aki
A
Bootim
Nitaya
N
Yano
Shigekazu
S
Tachiki
Takashi
T
Yoshimune
Kazuaki
K
Moriguchi
Mitsuaki
M
D000595:Amino Acid Sequence; D001216:Asparagine / Q000378:metabolism; D001236:Aspergillus oryzae / Q000201:enzymology; D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology; D005285:Fermentation; D018698:Glutamic Acid / Q000378:metabolism; D005972:Glutaminase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D016982:Micrococcus luteus / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology; D012462:Saline Solution, Hypertonic; D045730:Soy Foods; D020615:Stenotrophomonas maltophilia / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012462:Saline Solution, Hypertonic; D005973:Glutamine; D018698:Glutamic Acid; D001216:Asparagine; D005972:Glutaminase; C477762:salt-tolerant glutaminase