Aldosterone inhibits uncoupling protein-1, induces insulin resistance, and stimulates proinflammatory adipokines in adipocytes.
Aldosterone is a mineralocorticoid hormone that regulates blood pressure and salt/water balance. Increased aldosterone levels are found in states of disturbed energy balance such as the metabolic syndrome. Adipose tissue has been recognized to play a pivotal role in the regulation of energy homeostasis. We investigated direct aldosterone effects on brown adipocyte function. Aldosterone dose-dependently inhibited expression of uncoupling protein-1 (UCP-1) by 30% (p < 0.01). Furthermore, aldosterone dose-dependently impaired insulin-induced glucose uptake by about 25% (p < 0.01). On a transcriptional level, mRNA of the proinflammatory adipokines leptin and monocyte chemoattractant protein-1 (MCP-1) was increased by 5,000% and 40%, respectively, by aldosterone exposure (p < 0.05). This study demonstrates that aldosterone directly impacts on major adipose functions including stimulation of proinflammatory adipokines.
Kraus
D
D
Department of Internal Medicine I, University of Lübeck, 23538 Lübeck, Germany.
Jäger
J
J
Meier
B
B
Fasshauer
M
M
Klein
J
J
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D018932:Chemokine CCL2 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D007249:Inflammation / Q000473:pathology*; D007301:Insect Hormones / Q000096:biosynthesis*; D007333:Insulin Resistance / Q000502:physiology*; D007473:Ion Channels; D020738:Leptin / Q000096:biosynthesis; D008565:Membrane Proteins / Q000037:antagonists & inhibitors*; D051379:Mice; D024101:Mitochondrial Proteins; D009842:Oligopeptides / Q000096:biosynthesis*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives*; D012333:RNA, Messenger / Q000096:biosynthesis; D022722:Thermogenesis / Q000187:drug effects; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D018932:Chemokine CCL2; D007301:Insect Hormones; D007473:Ion Channels; D020738:Leptin; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009842:Oligopeptides; D012333:RNA, Messenger; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; C044833:adipokinetic hormone; D000450:Aldosterone; D005947:Glucose; D011761:Pyrrolidonecarboxylic Acid