title: Isogenic autosomes to be applied in optimal screening for novel mutants with viable phenotypes in Drosophila melanogaster.
abstract: Most insertional mutagenesis screens of Drosophila performed to date have not used target chromosomes that have been checked for their suitability for phenotypic screens for viable phenotypes. To address this, we have generated a selection of stocks carrying either isogenized second chromosomes or isogenized third chromosomes, in a genetic background derived from a Canton-S wild-type strain. We have tested these stocks for a range of behavioral and other viable phenotypes. As expected, most lines are statistically indistinguishable from Canton-S in most phenotypes tested. The lines generated are now being used as target chromosomes in mutagenesis screens, and the characterization reported here will facilitate their use in screens of these lines for behavioral and other viable phenotypes.
authors: Department of Genetics, University of Cambridge, Cambridge, UK.
authors: Punita
authors: P
authors: Sharma
authors: Zoltan
authors: Z
authors: Asztalos
authors: Champakali
authors: C
authors: Ayyub
authors: Marien
authors: M
authors: de Bruyne
authors: Anthony J
authors: AJ
authors: Dornan
authors: Araceli
authors: A
authors: Gomez-Hernandez
authors: John
authors: J
authors: Keane
authors: James
authors: J
authors: Killeen
authors: Susanne
authors: S
authors: Kramer
authors: Mayur
authors: M
authors: Madhavan
authors: Helen
authors: H
authors: Roe
authors: Pradeep Dagadu
authors: PD
authors: Sherkhane
authors: Khalid
authors: K
authors: Siddiqi
authors: Elizabeth
authors: E
authors: Silva
authors: John R
authors: JR
authors: Carlson
authors: Stephen F
authors: SF
authors: Goodwin
authors: Martin
authors: M
authors: Heisenberg
authors: Kits
authors: K
authors: Krishnan
authors: Charalambos P
authors: CP
authors: Kyriacou
authors: Linda
authors: L
authors: Partridge
authors: Juan
authors: J
authors: Riesgo-Escovar
authors: Veronica
authors: V
authors: Rodrigues
authors: Tim
authors: T
authors: Tully
authors: Cahir J
authors: CJ
authors: O'Kane
mesh_terms: D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001547:Benzaldehydes / Q000494:pharmacology; D002940:Circadian Rhythm / Q000235:genetics; D003307:Copulation; D004251:DNA Transposable Elements / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005820:Genetic Testing / Q000379:methods; D018404:Isochromosomes / Q000235:genetics*; D007858:Learning / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D008297:Male; D009154:Mutation; D010243:Paralysis / Q000235:genetics; D010641:Phenotype; D012726:Sexual Behavior, Animal; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D000777:Anesthetics; D001547:Benzaldehydes; D004251:DNA Transposable Elements; C032175:benzaldehyde