[Ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen].
OBJECTIVE
To investigate the ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen.


METHODS
ELISA was used to determine the inhibitory effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen in vitro. After ginseng glycopeptide was intraperitoneally administrated to streptozotocin-induced diabetic rats for 12 weeks, the acid solubility, limited pepsin degradation properties and solubility in SDS-2-mercaptoethanol of the rat tail tendon collagen were determined, and the effect of ginseng glycopeptide on the tail tendon collagen cross-linking was evaluated.


RESULTS
Ginseng glycopeptide inhibited significantly the cross-linking of rat tail tendon collagen in vitro. The solubility of the tail tendon collagen (in acid, pepsin and SDS-2-mercaptoethanol) was markedly decreased in diabetic rats and ginseng glycopeptide-treated diabetic rats had significantly an increase in the collagen solubility in the above-mentioned solutions, suggesting that ginseng glycopeptide decreased severity of the collagen cross-linking.


CONCLUSIONS
Ginsengglycopeptide exhibits an significantly ameliorative effect on cross-linking of rat tail tendon collagen.
Li
Cai
C
Institute of Frontier Medical Science, Jilin University, Changchun 130021, China. licaijia@public.cc.jl.cn
Li
Xiang-jun
XJ
Miao
Chun-sheng
CS
Zhang
Xiu-yun
XY
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013623:Tail; D013710:Tendons / Q000378:metabolism*
D006020:Glycopeptides; D003094:Collagen