Anti-tumour potential of a gallic acid-containing phenolic fraction from Oenothera biennis.
A phenolic fraction purified form defatted seeds of Oenothera biennis promoted selective apoptosis of human and mouse bone marrow-derived cell lines following first-order kinetics through a caspase-dependent pathway. In non-leukemia tumour cell lines, such as human colon carcinoma CaCo(2) cells and mouse fibrosarcoma WEHI164 cells, this fraction inhibited (3)H-thymidine incorporation but not cell death or cell cycle arrest. Human peripheral blood mononuclear cells showed low sensitivity to treatment. Single bolus injection of the phenolic fraction could delay the growth of established myeloma tumours in syngeneic animals. HPLC and mass spectrometry analysis revealed that the fraction contains gallic acid. However, the biological activity of the fraction differs from the activity of this phenol and hence it should be attributed to other co-purified molecules which remain still unidentified.
Pellegrina
Chiara Dalla
CD
Dipartimento Scientifico e Tecnologico, Facolta' di Scienze MM.FF.NN., Universita' di Verona, Strada Le Grazie, 15 CV-1, I-37134 Verona, Italia.
Padovani
Giorgia
G
Mainente
Federica
F
Zoccatelli
Gianni
G
Bissoli
Gaetano
G
Mosconi
Silvia
S
Veneri
Gianluca
G
Peruffo
Angelo
A
Andrighetto
Giancarlo
G
Rizzi
Corrado
C
Chignola
Roberto
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D020169:Caspases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000188:drug therapy; D031665:Oenothera biennis / Q000737:chemistry*; D010636:Phenols; D010936:Plant Extracts / Q000494:pharmacology*; D014183:Transplantation, Heterologous
D010636:Phenols; D010936:Plant Extracts; D005707:Gallic Acid; D020169:Caspases