title: Proinflammatory liver and antiinflammatory intestinal mediators involved in portal hypertensive rats.
abstract: Proinflammatory (TNF-alpha , IL-1beta, and NO) and antiinflammatory (IL-10, CO) levels were assayed in serum, liver, and small bowel in order to verify a hypothetic inflammatory etiopathogeny of portal hypertension that could be the cause of its evolutive heterogeneity. Male Wistar rats were divided into one control group (n=11) and one group with a triple stenosing ligation of the portal vein (n=23) after 28 days of evolution. In one subgroup of portal hypertensive rats, portal pressure, collateral venous circulation, mesenteric vasculopathy, and liver and spleen weights were determined. In the remaining rats with portal hypertension TNF-alpha, IL-1beta, and IL-10 were quantified in liver and ileum by enzyme-linked immunosorbent assay. NO synthase activity was studied in liver and ileum. CO and NO were measured in portal and systemic blood by spectrophotometry and Griess reaction, respectively. Portal hypertensive rats with mayor spleen weight show hepatomegaly and mayor development of collateral circulation. Ileum release of IL-10 (0.30 +/- 0.12 versus 0.14 +/- 0.02 pmol/mg protein; P< .01) is associated with a liver production of both proinflammatory mediators (TNF-alpha: 2 +/- 0.21 versus 1.32 +/- 0.60 pmol/mg protein; P< .05, IL-1beta: 19.17 +/- 2.87 versus 5.96 +/- 1.84 pmol/mg protein; P=.005, and NO: 132.10 +/- 34.72 versus 61.05 +/- 8.30 nmol/mL; P=.005) and an antiinflammatory mediator (CO: 6.49 +/- 2.99 versus 3.03 +/- 1.59 pmol/mL; P=.005). In short-term prehepatic portal hypertension a gut-liver inflammatory loop, which could be fundamental in the regulation both of the portal pressure and of its complications, could be proposed.
authors: Department of Surgery, Complutense University of Madrid, Spain. maaller@med.ucm.es
authors: Maria Angeles
authors: MA
authors: Aller
authors: Elena
authors: E
authors: Vara
authors: Cruz
authors: C
authors: Garcia
authors: Maria Dolores
authors: MD
authors: Palma
authors: Jorge L
authors: JL
authors: Arias
authors: Maria Paz
authors: MP
authors: Nava
authors: Jaime
authors: J
authors: Arias
mesh_terms: D000818:Animals; D001835:Body Weight; D002248:Carbon Monoxide / Q000097:blood; D003097:Collateral Circulation; D006975:Hypertension, Portal / Q000209:etiology*; D018836:Inflammation Mediators / Q000502:physiology*; D007375:Interleukin-1 / Q000502:physiology*; D016753:Interleukin-10 / Q000502:physiology*; D007422:Intestines / Q000502:physiology*; D008099:Liver / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D018836:Inflammation Mediators; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; D002248:Carbon Monoxide; D019001:Nitric Oxide Synthase