title: Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea.
abstract: Vibrio fluvialis is a halophilic Vibrio species associated with acute diarrhoeal illness in humans. It has the potential to cause outbreaks and has an association with paediatric diarrhoea. In this study, 11 V. fluvialis strains isolated from hospitalized patients with acute diarrhoea at the Infectious Diseases Hospital, Kolkata were extensively characterized. All the strains showed growth in peptone broth containing 7% NaCl. The strains showed variable results in Voges-Proskauer test and to a vibriostatic agent. There was also variation in their antibiograms, and some of the strains were multidrug resistant. Among the 11 strains, two showed only a single band difference in their PFGE profile and the remaining strains showed nine different PFGE patterns. However, unlike PFGE, the strains exhibited close matches and clustering in their ribotype patterns. The haemolytic effect on sheep red blood cells varied with strains. Partial sequence analysis revealed that the V. fluvialis haemolysin gene has 81% homology with that of the El Tor haemolysin of Vibrio cholerae. A striking finding was the capability of all the strains to evoke distinct cytotoxic and vacuolation effects on HeLa cells.
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Rupa
authors: R
authors: Chakraborty
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Subhra
authors: S
authors: Chakraborty
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Keya
authors: K
authors: De
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Sutapa
authors: S
authors: Sinha
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Asish K
authors: AK
authors: Mukhopadhyay
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Jasmina
authors: J
authors: Khanam
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Thandavarayan
authors: T
authors: Ramamurthy
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Yoshifumi
authors: Y
authors: Takeda
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: Sujit K
authors: SK
authors: Bhattacharya
authors: National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.
authors: G Balakrish
authors: GB
authors: Nair
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis / Q000494:pharmacology*; D002771:Cholera / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology*; D006367:HeLa Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D014617:Vacuoles / Q000187:drug effects*; D014733:Vibrio / Q000145:classification / Q000472:pathogenicity* / Q000502:physiology; D014734:Vibrio cholerae / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins