title: Application of preparative high-speed counter-current chromatography for isolation and separation of schizandrin and gomisin A from Schisandra chinensis.
abstract: Following an initial cleaning-up step on the D101 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.9:0.9:1, v/v) was used to isolate and separate schizandrin and gomisin A from Schisandra chinensis. A total of 107 mg schizandrin and 36 mg gomisin A with purities of 99.5% and 99.1% were obtained from 400 mg crude extract in one-step elution and less than 3 h, and the structure identification was performed by UV, IR, MS, 1H NMR and 13C NMR.
authors: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.
authors: Jinyong
authors: J
authors: Peng
authors: Guorong
authors: G
authors: Fan
authors: Liping
authors: L
authors: Qu
authors: Xin
authors: X
authors: Zhou
authors: Yutian
authors: Y
authors: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D034242:Cyclooctanes / Q000302:isolation & purification*; D004149:Dioxoles / Q000302:isolation & purification*; D017705:Lignans / Q000302:isolation & purification*; D011083:Polycyclic Compounds / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; C033585:schizandrol B; C011105:schizandrin