title: [Effects of cyclosporin A on gene expression profiles of NIT-1 pancreatic beta cell line].
abstract: OBJECTIVE
  To observe the effects of cyclosporin A (CsA) on gene expression profiles of NIT-1 pancreatic beta cells cell line using microarray technique.
  
  
  METHODS
  NIT-1 cells were exposed to cyclosporin A treatment (10 micromol/L) for 24 h and the differential gene expressions were assessed using microarray technique.
  
  
  RESULTS
  After 24h of CsA treatment, 38 of the 4096 genes tested were up-regulated, including 13 genes with known functions involving stress response, cell growth and protein synthesis. Meanwhile 46 genes were down-regulated, including 25 genes with known functions involving cell growth and maturation, oxidative phosphorylation and protein synthesis. Changes of gene expression of Zfr,Tpi and Pax6 were confirmed by semi-quantitative reverse transcription polymerase chain reaction.
  
  
  CONCLUSIONS
  CsA treatment for 24 h induces changes in the gene expression profiles of NIT-1 pancreatic beta cells. Down-regulation of the genes related to cell growth and maturation, oxidative phosphorylation and protein synthesis may partly explain the mechanisms that CsA inhibits the release of insulin from pancreatic beta cells.
authors: Institute of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. jiazm@fimmu.com
authors: Zhi-min
authors: ZM
authors: Jia
authors: Wei
authors: W
authors: Xu
authors: Le
authors: L
authors: Yu
authors: Jia-jie
authors: JJ
authors: Zhang
authors: Lin
authors: L
authors: Lü
authors: Lin-sheng
authors: LS
authors: Lei
authors: Shu-guang
authors: SG
authors: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D016572:Cyclosporine