Application of supercritical fluid chromatography in the quantitative analysis of minor components (carotenes, vitamin E, sterols, and squalene) from palm oil.
The application of supercritical fluid chromatography (SFC) coupled with a UV variable-wavelength detector to isolate the minor components (carotenes, vitamin E, sterols, and squalene) in crude palm oil (CPO) and the residual oil from palm-pressed fiber is reported. SFC is a good technique for the isolation and analysis of these compounds from the sources mentioned. The carotenes, vitamin E, sterols, and squalene were isolated in less than 20 min. The individual vitamin E isomers present in palm oil were also isolated into their respective components, alpha-tocopherol, alpha-tocotrienol, gamma-tocopherol, gamma-tocotrienol, and delta-tocotrienol. Calibration of all the minor components of palm as well as the individual components of palm vitamin E was carried out and was found to be comparable to those analyzed by other established analytical methods.
Choo
Yuen May
YM
Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. Choo@mpob.gov.my
Ng
Mei Han
MH
Ma
Ah Ngan
AN
Chuah
Cheng Hock
CH
Hashim
Mohd Ali
MA
D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D000073878:Palm Oil; D010840:Phytosterols / Q000032:analysis* / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D013185:Squalene / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014810:Vitamin E / Q000032:analysis* / Q000737:chemistry
D010840:Phytosterols; D010938:Plant Oils; D014810:Vitamin E; D002338:Carotenoids; D000073878:Palm Oil; D013185:Squalene