The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion.
The lineage of dendritic cells (DC), and in particular their relationship to monocytes and macrophages, remains obscure. Furthermore, the requirement for the macrophage growth factor CSF-1 during DC homeostasis is unclear. Using a transgenic mouse in which the promoter for the CSF-1R (c-fms) directs the expression of enhanced GFP in cells of the myeloid lineage, we determined that although the c-fms promoter is inactive in DC precursors, it is up-regulated in all DC subsets during differentiation. Furthermore, plasmacytoid DC and all CD11c(high) DC subsets are reduced by 50-70% in CSF-1-deficient osteopetrotic mice, confirming that CSF-1 signaling is required for the optimal differentiation of DC in vivo. These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo.
MacDonald
Kelli P A
KP
Bone Marrow Transplantation Laboratory, Queensland Institute of Medical Research, Queensland, Australia. kelliM@qimr.edu.au
Rowe
Vanessa
V
Bofinger
Helen M
HM
Thomas
Ranjeny
R
Sasmono
Tedjo
T
Hume
David A
DA
Hill
Geoffrey R
GR
D000818:Animals; D001772:Blood Cell Count; D039521:CD11c Antigen / Q000096:biosynthesis / Q000097:blood; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016173:Macrophage Colony-Stimulating Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology
D039521:CD11c Antigen; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor; D017493:Leukocyte Common Antigens