title: Dysmorphic neuromuscular junctions associated with motor ability in cerebral palsy.
abstract: Cerebral palsy (CP) is the most prevalent neurologic disease in children and a leading cause of severe physical disability. Research and clinical experience indicate that children with CP have abnormal neuromuscular junctions (NMJs), and we present evidence that nonapposition of neuromuscular junction components is associated with the severity of motor system deficit in CP. Leg muscle biopsies collected from ambulatory (n = 21) or nonambulatory (n = 38) CP patients were stained in order to detect acetylcholine receptor (AChR) and acetylcholine esterase (AChE). Image analysis was used to calculate the extra-AChE spread (EAS) of AChR staining to estimate the amount of AChR occurring outside the functional, AChE-delimited NMJ. Nonambulatory children exhibited higher average EAS (P = 0.025) and had a greater proportion of their NMJs with significantly elevated EAS (P = 0.023) than ambulatory children. These results indicate that physical disability in children with CP is associated with structurally dysmorphic NMJs, which has important implications for the management of CP patients, especially during surgery and anesthesia.
authors: Nemours Biomedical Research, AI duPont Hospital for Children, Wilmington, Delaware 19803, USA.
authors: Mary C
authors: MC
authors: Theroux
authors: Karyn G
authors: KG
authors: Oberman
authors: Justine
authors: J
authors: Lahaye
authors: Bobbie A
authors: BA
authors: Boyce
authors: David
authors: D
authors: Duhadaway
authors: Freeman
authors: F
authors: Miller
authors: Robert E
authors: RE
authors: Akins
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D002547:Cerebral Palsy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D009469:Neuromuscular Junction / Q000378:metabolism* / Q000473:pathology*; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: D011978:Receptors, Nicotinic; D000110:Acetylcholinesterase