[Isolation, characterization and expression analysis of male sterility gene homology sequence in wheat].
One 134 bp fragment was amplified in anthers of male sterile and fertile wheat using one pair of degenerated primer designed based on the conserved domain of MS2 gene in Arabidopsis thaliana and Oryza sativa, and one 1604 bp male sterility gene homology sequence was extended by in silico cloning based on the 134 bp fragment. The amino acids encoded by the male sterility gene homology sequence include a 200 amino acid conserved domain of male sterility, and this sequence expressed only in wheat male fertile anthers; no expression in male sterile anthers, roots and leaves. This research demonstrated that the cloned male sterile homology sequence is specific to wheat anther development.
Chen
Jun-Fang
JF
Key Lab of Crop Germplasm & Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Ren
Zheng-Long
ZL
Kong
Xiu-Ying
XY
Wu
Jia-Jie
JJ
Zhou
Rong-Hua
RH
Jia
Ji-Zeng
JZ
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D051479:Plant Infertility / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant