Analysis of pulegone and its enanthiomeric distribution in mint-flavoured food products.
A procedure for the extraction and determination of pulegone enanthiomers in mint essential oils and mint products (syrups, dried leaves, toothpaste, lozenges, candy and chewing-gum) was developed. The compounds were recovered from the food matrices by employing a simultaneous distillation-extraction (SDE) technique with a Likens-Nickerson apparatus using dichloromethane as an extraction solvent. The analyses were performed by capillary gas chromatography mass spectrometry (GC/MS). Experiments on food products spiked at different pulegone concentrations showed recoveries ranging from 95 to 106%. The detection limit was about 5?mg?l(-1) for both pulegone enanthiomers and good linearity was found in the concentration range 0.5-25?mg?l(-1). In a number of repeated analyses, the pulegone peak height repeatability (RSD) was 0.2%. The pulegone enanthiomers were separated and quantified by enanthioselective multidimensional gas chromatography. The results of analyses conducted on essential mint oils and mint-flavoured food products are reported.
Siano
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Stazione Sperimentale per le Industrie delle Essenze e dei Derivati dagli Agrumi, Via Gen. Tommasini 2, I-89127 Reggio Calabria, Italy.
Catalfamo
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M
Cautela
D
D
Servillo
L
L
Castaldo
D
D
D000081005:Cyclohexane Monoterpenes; D005421:Flavoring Agents / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D019649:Food Industry / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D027541:Mentha / Q000737:chemistry*; D036142:Mentha piperita / Q000737:chemistry; D039821:Monoterpenes / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry
D000081005:Cyclohexane Monoterpenes; D005421:Flavoring Agents; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; C039648:pulegone