title: Evaluation of the Konelab 20XT clinical chemistry analyzer.
abstract: The Konelab 20XT (Thermo Electron Oy, Finland) is a clinical chemistry analyzer for colorimetric, immunoturbidimetric and ion-selective electrode measurements. The aim of our work was to evaluate the analytical performances of the Konelab 20XT according to the European Clinical Chemistry Laboratory Standards Guidelines. A total of 30 analytes including substrates, enzymes, electrolytes and specific proteins were tested. Investigation results showed low imprecision (within-run coefficient of variation was below 3.5% and between-day coefficient of variation was less than 2.5% for most analytes at all three levels studied) and acceptable accuracy of the analyzer. No significant sample- or reagent-related carry-over was found. It was demonstrated that the analytical system operates within the claimed linearity ranges. The results compared well with those obtained by instruments routinely used in our laboratory (Olympus AU2700, Behring Nephelometer II). In general, the data on interference by hemoglobin, hyperbilirubinemia and turbidity are in accordance with known facts. However, slight hemolysis was found to interfere with the alkaline phosphatase (ALP) assay and mild lipemia affected the glucose assay. The Konelab 20XT is an easy-to-use analyzer that is suitable for routine and emergency analyses in small laboratories.
authors: Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
authors: Natasa
authors: N
authors: Stojanović
authors: Dunja
authors: D
authors: Rogić
authors: Ana
authors: A
authors: Stavljenić-Rukavina
mesh_terms: D002138:Calibration; D002624:Chemistry, Clinical / Q000295:instrumentation*; D003124:Colorimetry; D004566:Electrodes; D006801:Humans; D007118:Immunoassay; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results