ASF/SF2 and SC35 regulate the glutamate receptor subunit 2 alternative flip/flop splicing.
The properties of the glutamate receptor subunits 1-4 (GluR1-4) are influenced by the alternative splicing of two homologous and mutually exclusive exons flip and flop. The flip form is most abundant during early development, while the flop form is dominant in adults. From transfections with a GluR2 mini-gene we show that flip is the preferred splice form in all tested cell lines, but coexpression of the SR-proteins ASF/SF2 and SC35 increases the flop to flip splice ratio. The increased flop incorporation depends on ASF/SF2- and SC35-dependent enhancer elements located in the flop exon, which stimulate the splicing between the flop exon and the preceding exon 13.
Crovato
Trine Elkjaer
TE
Department of Molecular Biology, University of Aarhus, C.F. Møllers Alle 130, DK-8000 Aarhus C, Denmark. tel@mb.au.dk
Egebjerg
Jan
J
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA; D005091:Exons; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017470:Receptors, Glutamate / Q000235:genetics / Q000378:metabolism*
D016601:RNA-Binding Proteins; D017470:Receptors, Glutamate; D004247:DNA