title: Dephosphorylation of the Rieske iron-sulfur protein after induction of the mitochondrial permeability transition.
abstract: In the mitochondrial permeability transition (MPT), MPT pores open to cause the mitochondrial inner membrane to become non-selectively permeable to molecules of mass up to 1500 Da. In this study, we used proteomics to investigate protein changes after MPT induction. Isolated rat liver mitochondria were incubated with various MPT inducers, including CaCl2, tert-butylhydroperoxide, and phenylarsine oxide, in the presence and absence of the MPT inhibitor, cyclosporin A. MPT induction was confirmed by an absorbance swelling assay. Mitochondrial membrane proteins prepared from control and treated mitochondria were separated by two-dimensional (2D) gel electrophoresis and stained with SyproRuby or Coomassie blue. Proteins of interest were further identified by mass spectrometry. 2D gel electrophoresis by isoelectric focusing and SDS-PAGE consistently showed a protein spot that shifted to a more basic isoelectric point after the MPT. This shift was prevented by CsA but did not occur after protonophoric uncoupling. Mass spectrometry identified this protein as the Rieske iron-sulfur protein (RISP) of ubiquinol-cytochrome c reductase (Complex III). Phosphatase treatment of sonicated mitochondria caused the same shift in RISP as occurred in MPT inducer-treated mitochondria. 2D gel electrophoresis by blue-native-PAGE and SDS-PAGE showed that RISP existed as an apparent monomer in mitochondrial membranes in addition to forming a complex with ubiquinol-cytochrome c reductase. These findings suggest that RISP may be part of MPT pores and that dephosphorylation of RISP may play a role in regulation of the MPT.
authors: Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599-7090, USA.
authors: Lihua
authors: L
authors: He
authors: John J
authors: JJ
authors: Lemasters
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D014450:Electron Transport Complex III / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D051336:Mitochondrial Membranes / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010539:Permeability / Q000187:drug effects; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D001152:Arsenicals; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; C029341:oxophenylarsine; D016572:Cyclosporine; D020122:tert-Butylhydroperoxide; D010749:Phosphoprotein Phosphatases; D014450:Electron Transport Complex III; D002122:Calcium Chloride