ELISA test for anti-neutrophil cytoplasm antibodies detection evaluated by a computer screen photo-assisted technique.
The computer screen photo-assisted technique (CSPT), a method for substance classification based on spectral fingerprinting, which involves just a computer screen and a web camera as measuring platform is used here for the evaluation of a prospective enzyme-linked immunosorbent assay (ELISA). A anti-neutrophil cytoplasm antibodies (ANCA-ELISA) test, typically used for diagnosing patients suffering from chronic inflammatory disorders in the skin, joints, blood vessels and other tissues is comparatively tested with a standard microplate reader and CSPT, yielding equivalent results at a fraction of the instrumental costs. The CSPT approach is discussed as a distributed measuring platform allowing decentralized measurements in routine applications, whereas keeping centralized information management due to its natural network embedded operation.
Filippini
D
D
Division of Applied Physics, Institute of Physics and Measurement Technology, Linköping University, S-58183 Linköping, Sweden. danfi@ifm.liu.se
Tejle
K
K
Lundström
I
I
D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D003626:Data Display; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D020407:Internet; D010781:Photography / Q000295:instrumentation / Q000379:methods*; D010783:Photometry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014584:User-Computer Interface; D014741:Video Recording / Q000295:instrumentation / Q000379:methods
D019268:Antibodies, Antineutrophil Cytoplasmic