title: Junctional adhesion molecule-A-deficient polymorphonuclear cells show reduced diapedesis in peritonitis and heart ischemia-reperfusion injury.
abstract: Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A is required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or in the heart upon ischemia-reperfusion injury. The defect was not observed in mice with an endothelium-restricted deficiency of the protein but was still detectable in mice transplanted with bone marrow from JAM-A(-/-) donors. Microscopic examination of mesenteric and heart microvasculature of JAM-A(-/-) mice showed high numbers of PMN adherent on the endothelium or entrapped between endothelial cells and the basement membrane. In vitro, in the absence of JAM-A, PMN adhered more efficiently to endothelial cells and basement membrane proteins, and their polarized movement was strongly reduced. This paper describes a nonredundant role of JAM-A in controlling PMN diapedesis through the vessel wall.
authors: Fondazione Italiana per la Ricerca sul Cancro, Institute of Molecular Oncology, 20139 Milan, Italy.
authors: Monica
authors: M
authors: Corada
authors: Stefano
authors: S
authors: Chimenti
authors: Maria Rosaria
authors: MR
authors: Cera
authors: Maria
authors: M
authors: Vinci
authors: Monica
authors: M
authors: Salio
authors: Fabio
authors: F
authors: Fiordaliso
authors: Noeleen
authors: N
authors: De Angelis
authors: Antonello
authors: A
authors: Villa
authors: Mario
authors: M
authors: Bossi
authors: Lidia I
authors: LI
authors: Staszewsky
authors: Annunciata
authors: A
authors: Vecchi
authors: Dario
authors: D
authors: Parazzoli
authors: Toshiyuki
authors: T
authors: Motoike
authors: Roberto
authors: R
authors: Latini
authors: Elisabetta
authors: E
authors: Dejana
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000172:deficiency*; D002465:Cell Movement / Q000502:physiology; D004730:Endothelium, Vascular / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D010538:Peritonitis / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules