In vivo and in vitro proliferative and differentiation activity of human embryonic retinal cells.
Differentiation of human embryonic retinal cells (20-22 weeks gestation) was studied using morphological, immunohistochemical, and biomolecular approaches. The retina included several regions differing by the degree of cell differentiation. Mitoses were rarely found in the marginal zone. This zone contained low differentiated cells. The central retinal area consisted of typical layers with differentiated cells. Culturing was accompanied by the formation of aggregates and neurospheres, where mitoses and progenitor or differentiated cells expressing markers of photoreceptors, neurons, and glia were found.
Panova
I G
IG
N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Russia. pinag@mail.ru
Podgornyi
O V
OV
Verdiev
B
B
Smirnova
Yu A
YA
Poltavtseva
R A
RA
Grigoryan
E N
EN
Zinov'eva
R D
RD
Aleksandrova
M A
MA
Sukhikh
G T
GT
Mitashov
V I
VI
D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051603:Recoverin / Q000235:genetics; D012160:Retina / Q000166:cytology* / Q000196:embryology*; D014404:Tubulin / Q000235:genetics; D014746:Vimentin / Q000235:genetics
D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D014404:Tubulin; D014746:Vimentin; D051603:Recoverin