Determination of the relative amounts of Gag and Pol proteins in foamy virus particles.
We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68Gag moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71Gag plus p68Gag:p85RT),12:1 (p68Gag:p85RT), and 10:1 (pr71Gag plus p68Gag:p40IN). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.
Cartellieri
Marc
M
Institut für Virologie, Medizinische Fakultät, Technische, Universität Dresden, Germany. Marc.Cartellieri@mailbox.tu-dresden.de
Rudolph
Wolfram
W
Herchenröder
Ottmar
O
Lindemann
Dirk
D
Rethwilm
Axel
A
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D002213:Capsid / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism*; D015684:Gene Products, pol / Q000378:metabolism*; D015751:Genes, pol; D011994:Recombinant Proteins / Q000378:metabolism; D016092:Spumavirus / Q000378:metabolism* / Q000472:pathogenicity; D014771:Virion / Q000378:metabolism* / Q000472:pathogenicity
D015683:Gene Products, gag; D015684:Gene Products, pol; D011994:Recombinant Proteins