Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA.
Five isolates of Cucumber mosaic virus (CMV) from Lilium sp. (lily), which were isolated from specimens in Japan, Korea and Taiwan, were unable to support satellite RNA (satRNA) accumulation. In order to map the CMV sequences that are involved in satRNA support, HL-CMV (Japanese lily isolate), Y-CMV (ordinary strain) and Y-satellite RNA (Y-sat) were used as the source material. The pseudorecombinants between Y-CMV and HL-CMV revealed that RNA1 was essential for satRNA replication in lily. The results of chimeric constructs and various mutations showed that two amino acid residues (at positions 876 and 891) in the 1a protein were the determinants for the inability of HL-CMV to support a satRNA. Specifically, Thr at position 876 had a more pronounced effect than Met at position 891. Specific changes in RNA sequence were also detected in the 3' terminus of Y-sat and these particular alterations allowed it to be supported by HL-CMV. It is believed that, through evolution, the adaptation of CMV to lily resulted in the introduction of amino acid changes in the 1a protein, changes that coincidentally affected the ability of lily CMV to support satRNAs.
Yamaguchi
Naoya
N
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Seshimo
Yuko
Y
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Yoshimoto
Eri
E
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Ahn
Hong Il
HI
Graduate School of Biotechnology, Korea University, Seoul 139-774, Korea.
Ryu
Ki Hyun
KH
Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University, Seoul, 139-774, Korea.
Choi
Jang Kyung
JK
Division of Biological Environment, Kangwon National University, Chunchon 200-701, Korea.
Masuta
Chikara
C
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D019401:Cucumber Mosaic Virus Satellite / Q000096:biosynthesis*; D017799:Cucumovirus / Q000235:genetics / Q000502:physiology*; D006378:Helper Viruses / Q000235:genetics / Q000502:physiology*; D007564:Japan; D007723:Korea; D027762:Lilium / Q000821:virology*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D013624:Taiwan; D014764:Viral Proteins / Q000235:genetics
D019401:Cucumber Mosaic Virus Satellite; D012367:RNA, Viral; D014764:Viral Proteins