title: Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint.
abstract: Perlecan is a modular heparan sulphate and/or chondroitin sulphate substituted proteoglycan of basement membrane, vascular tissues and cartilage. Perlecan acts as a low affinity co-receptor for fibroblast growth factors 1, 2, 7, 9, binds connective tissue growth factor and co-ordinates chondrogenesis, endochondral ossification and vascular remodelling during skeletal development; however, relatively little is known of its distribution in these tissues during ageing and development. The aim of the present study was to immunolocalise perlecan in the articular and epiphyseal growth plate cartilages of stifle joints in 2-day to 8-year-old pedigree merino sheep. Perlecan was prominent pericellularly in the stifle joint cartilages at all age points and also present in the inter-territorial matrix of the newborn to 19-month-old cartilage specimens. Aggrecan was part pericellular, but predominantly an extracellular proteoglycan. Perlecan was a prominent component of the long bone growth plates and displayed a pericellular as well as a strong ECM distribution pattern; this may indicate a so far unrecognised role for perlecan in the mineralisation of hypertrophic cartilage. A significant age dependant decline in cell number and perlecan levels was evident in the hyaline and growth plate cartilages. The prominent pericellular distribution of perlecan observed indicates potential roles in cell-matrix communication in cartilage, consistent with growth factor signalling, cellular proliferation and tissue development.
authors: Raymond Purves Laboratory, Institute of Bone and Joint Research, University of Sydney at the Royal North Shore Hospital, St Leonards, NSW, 2065, Australia. jmelrose@med.usyd.edu.au
authors: James
authors: J
authors: Melrose
authors: Susan
authors: S
authors: Smith
authors: Martin
authors: M
authors: Cake
authors: Richard
authors: R
authors: Read
authors: John
authors: J
authors: Whitelock
mesh_terms: D053674:Aggrecans; D000818:Animals; D000831:Animals, Newborn; D002358:Cartilage, Articular / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006132:Growth Plate / Q000737:chemistry*; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis*; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D011509:Proteoglycans / Q000032:analysis; D012756:Sheep; D013264:Stifle / Q000737:chemistry*; D013997:Time Factors
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D037181:Lectins, C-Type; D011509:Proteoglycans; C071927:perlecan