title: Cytological properties of stromal cells derived from giant cell tumor of bone (GCTSC) which can induce osteoclast formation of human blood monocytes without cell to cell contact.
abstract: When human blood monocytes were cocultured with stromal cells derived from human giant cell tumor of bone (GCTSC) and a Millipore filter (0.4 microm) was interposed between monocytes and GCTSC, multinucleated giant cell formation of monocytes was induced. The multinucleated giant cells have characters as osteoclast-like cells, indicating that a soluble osteoclast-inducing factor(s) is secreted from GCTSC expressing RANK, RANKL/ODF/OPGL and TACE mRNA. Furthermore, OCIF/OPG inhibited GCTSC-induced osteoclastogenesis, showing that the RANK-RANKL system is involved in GCTSC-induced osteoclastogenesis and that soluble form of ODF/RANKL induces osteoclasts from monocytes. GCTSC expressed the cytokine mRNAs such as M-CSF, GM-CSF, IL-3, IL-4, IL-6, and IFN-gamma mRNAs. None of IL-1ralpha, IL-1alpha, IL-1beta, IL-2, IL-4, IL-10, IL-18, TNF-alpha, G-CSF and IFN-gamma could be detected in all culture media. A significant amount of IL-6 could be detected in the culture media of all GCTSC. IL-8 was found in the culture media of two GCTSC and two osteosarcoma-derived cells. M-CSF was detected in all culture media. GCTSC express CaSR, and stimulation of GCTSC with either extracellular Ca(2+) or neomycin, agonist of CaSR, augmented the expression of RANKL. Some lines of GCTSC expressed alkaline phosphatase, osteocalcin and Cbfa1, suggesting that GCTSC are intimately related to osteoblastic lineage.
authors: Department of Microbiology, Mie University School of Medicine, Tsu-Shi, Mie Prefecture 514-8507, Japan.
authors: Makoto
authors: M
authors: Nishimura
authors: Kimitaka
authors: K
authors: Yuasa
authors: Kouki
authors: K
authors: Mori
authors: Noriki
authors: N
authors: Miyamoto
authors: Morihiro
authors: M
authors: Ito
authors: Masato
authors: M
authors: Tsurudome
authors: Machiko
authors: M
authors: Nishio
authors: Mitsuo
authors: M
authors: Kawano
authors: Hiroshi
authors: H
authors: Komada
authors: Atsumasa
authors: A
authors: Uchida
authors: Yasuhiko
authors: Y
authors: Ito
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001859:Bone Neoplasms / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D002450:Cell Communication; D019070:Cell Lineage; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005870:Giant Cell Tumors / Q000473:pathology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D009000:Monocytes / Q000166:cytology*; D015675:Osteocalcin / Q000032:analysis; D010010:Osteoclasts / Q000502:physiology*; D010012:Osteogenesis; D053495:Osteopontin; D053245:RANK Ligand; D012333:RNA, Messenger / Q000032:analysis; D053246:Receptor Activator of Nuclear Factor-kappa B; D044169:Receptors, Calcium-Sensing / Q000235:genetics; D012795:Sialoglycoproteins / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*
chemical_list: C095550:CASR protein, human; D002352:Carrier Proteins; D016207:Cytokines; D008562:Membrane Glycoproteins; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D044169:Receptors, Calcium-Sensing; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human; D015675:Osteocalcin; D053495:Osteopontin; D000469:Alkaline Phosphatase