title: Curcumin inhibits interferon-alpha induced NF-kappaB and COX-2 in human A549 non-small cell lung cancer cells.
abstract: The A549 cells, non-small cell lung cancer cell line from human, were resistant to interferon (IFN)-alpha treatment. The IFN-alpha-treated A549 cells showed increase in protein expression levels of NF-kappaB and COX-2. IFN-alpha induced NF-kappaB binding activity within 30 min and this increased binding activity was markedly suppressed with inclusion of curcumin. Curcumin also inhibited IFN-alpha-induced COX-2 expression in A549 cells. Within 10 min, IFN-alpha rapidly induced the binding activity of a gamma-(32)P-labeled consensus GAS oligonucleotide probe, which was profoundly reversed by curcumin. Taken together, IFN-alpha-induced activations of NF-kappaB and COX-2 were inhibited by the addition of curcumin in A549 cells.
authors: Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors: Jeeyun
authors: J
authors: Lee
authors: Young-Hyuck
authors: YH
authors: Im
authors: Hae Hyun
authors: HH
authors: Jung
authors: Joo Hyun
authors: JH
authors: Kim
authors: Joon Oh
authors: JO
authors: Park
authors: Kihyun
authors: K
authors: Kim
authors: Won Seog
authors: WS
authors: Kim
authors: Jin Seok
authors: JS
authors: Ahn
authors: Chul Won
authors: CW
authors: Jung
authors: Young Suk
authors: YS
authors: Park
authors: Won Ki
authors: WK
authors: Kang
authors: Keunchil
authors: K
authors: Park
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage*; D051546:Cyclooxygenase 2; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage*; D008175:Lung Neoplasms / Q000378:metabolism*; D008565:Membrane Proteins; D016328:NF-kappa B / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*
chemical_list: D016898:Interferon-alpha; D008565:Membrane Proteins; D016328:NF-kappa B; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D003474:Curcumin