Reduced expressions of Foxp1 and Rassf1a genes in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in rats.
To clarify the involvement of the Foxp1 and Rassf1a genes in lung carcinogenesis, we investigated their expressions in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in rats. Six week old male Wistar rats were given 2000 ppm BHP in their drinking water for 12 weeks and maintained without further treatment until they were sacrificed at 25 weeks. A total of 10 lung adenocarcinomas were obtained, along with the total RNA from each for assessment of expression by reverse transcription (RT)-polymerase chain reaction (PCR). The reduced expressions of the Foxp1 and Rassf1a genes were observed in some of the lung adenocarcinomas. These analyses were also confirmed by real-time quantitative RT-PCR. These results suggest that reduced expressions of Foxp1 and Rassf1a genes may play a role in the development of lung adenocarcinomas induced by BHP in rats.
Shimizu
Kyoko
K
Laboratory of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Technology, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
Kato
Ayumi
A
Hinotsume
Daisei
D
Shigemura
Mayumi
M
Hanaoka
Madoka
M
Shimoichi
Yozo
Y
Honoki
Kanya
K
Tsujiuchi
Toshifumi
T
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009602:Nitrosamines; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D051858:Forkhead Transcription Factors; D009602:Nitrosamines; D012333:RNA, Messenger; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; C012457:diisopropanolnitrosamine