Hepatic morphological changes and oxidative stress in chronic streptozotocin-diabetic rats.
Oxidative stress (OS) is a biological entity quoted as responsible for several pathologies including diabetes. Diabetes mellitus (DM) has been also associated to human cirrhosis. The present work was designed to study the occurrence of OS as well as morphologic alterations in rat livers following induction of DM. Two groups of rats were used: Control and Diabetic. DM was induced in the second group by streptozotocin (STZ) in a single dose of 60 mg/kg, injected i.p. The occurrence of OS was determined in liver homogenates by measuring the hydroperoxide-initiated chemiluminescence and the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). Liver sinusoids were morphometrically analyzed. In conclusion, livers from the diabetic group did not show evidence of the occurrence of OS, as it would be expected, but dilation of hepatic sinusoids was documented and it was significantly different from control group.
Evelson
Pablo
P
Laboratorio de Estrés Oxidativo en Patologías Humanas (LEOPH). Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Susemihl
Carlota
C
Villarreal
Irene
I
Llesuy
Susana
S
Rodríguez
Ricardo
R
Peredo
Horacio
H
Lemberg
Abraham
A
Perazzo
Juan
J
Filinger
Ester
E
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002908:Chronic Disease; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000473:pathology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000473:pathology*; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase