title: Trans-scleral diffusion of triamcinolone acetonide.
abstract: OBJECTIVE
  To assess ex vivo human scleral permeability to triamcinolone acetonide (TA).
  
  
  METHODS
  The experiments were carried out using scleral samples and a Franz-type vertical diffusion cell. A suspension containing TA was prepared and placed in the donor chamber. The concentration of TA in the receptor chamber was measured by high-performance liquid chromatography (HPLC) assay and expressed as a percentage relative to TA concentration dissolved in the donor chamber. Control experiments using a commercial TA suspension were performed.
  
  
  RESULTS
  TA (+/-SEM) dissolved in the donor suspension was 10.69 +/- 1.28 microg/ml. The diffusion rate of TA varied from 30% after 1 day to 72% after 4 days, after which equilibrium was reached. The human scleral permeability coefficient (P(s) +/- SEM) was 1.47+/- 0.17 x 10(- 5) cm/s.
  
  
  CONCLUSIONS
  TA crossed human sclera. The mean amount of drug retained in the sclera increased with time, 4 days being necessary to equilibrate the unidirectional flux. The TA permeability coefficient was comparable to that of other corticosteroids.
authors: Jules Gonin Eye Hospital, University of Lausanne, Ocular Immunology, Lausanne, Switzerland.
authors: Paolo
authors: P
authors: Mora
authors: Simone
authors: S
authors: Eperon
authors: Olivia
authors: O
authors: Felt-Baeyens
authors: Robert
authors: R
authors: Gurny
authors: Serge
authors: S
authors: Sagodira
authors: Pascal
authors: P
authors: Breton
authors: Yan
authors: Y
authors: Guex-Crosier
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D005938:Glucocorticoids / Q000493:pharmacokinetics*; D006801:Humans; D008875:Middle Aged; D010539:Permeability; D012590:Sclera / Q000378:metabolism*; D014222:Triamcinolone Acetonide / Q000493:pharmacokinetics*
chemical_list: D005938:Glucocorticoids; D014222:Triamcinolone Acetonide