title: Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps.
abstract: CONCLUSIONS
  Our results indicate that the electrogenic kidney Na(+)/HCO(3)(-) cotransporter (kNBC), KCl cotransporter (KCC1 and -4) and Ca(2+)-activated Cl(-) channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells.
  
  
  OBJECTIVE
  Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. Transepithelial transport of ions and water in various fluid-transporting epithelia is strictly dependent on the localization of specific membrane proteins in the polarized epithelial cells. In this study we investigated the expression and distribution of mRNA transcripts for kNBC, pancreatic NBC, KCC1, -2, -3, -4 and CaCC1, -2, -3 gene families in human nasal mucosa and nasal polyp.
  
  
  METHODS
  The expression and localization of these gene families were investigated in inferior turbinate tissues and nasal polyp using reverse transcriptase polymerase chain reaction (RT-PCR), semiquantitative RT-PCR and in situ hybridization.
  
  
  RESULTS
  mRNAs for kNBC, KCC1 and -4 and all the CaCC families (CaCC1, -2 and -3) are expressed in human turbinate mucosa and nasal polyp. The expression levels of kNBC and KCC1 and -4 mRNAs did not differ between nasal mucosa and nasal polyp. However, the expression levels of all the CaCC genes were significantly decreased in nasal polyp. In situ hybridization revealed that the expression of these genes was mainly localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyp.
authors: Department of Otorhinolaryngology-Head & Neck Surgery, Colleges of Medicine, Korea University, Seoul, South Korea. sanghag@kumc.or.kr
authors: Sang Hag
authors: SH
authors: Lee
authors: Ji Hoon
authors: JH
authors: Park
authors: Hak Hyun
authors: HH
authors: Jung
authors: Seung Hoon
authors: SH
authors: Lee
authors: Joon Whan
authors: JW
authors: Oh
authors: Heung Man
authors: HM
authors: Lee
authors: Hyun Soo
authors: HS
authors: Jun
authors: Woo Jin
authors: WJ
authors: Cho
authors: Jae Yong
authors: JY
authors: Lee
mesh_terms: D000328:Adult; D001483:Base Sequence; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D017136:Ion Transport / Q000235:genetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism*; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027982:Sodium-Bicarbonate Symporters / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D014420:Turbinates / Q000737:chemistry; D000096922:K Cl- Cotransporters
chemical_list: D018118:Chloride Channels; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D027982:Sodium-Bicarbonate Symporters; D027981:Symporters; D000096922:K Cl- Cotransporters; C119651:SLC12A7 protein, human