Angiotensin II and interleukin-1 interact to increase generation of dopaminergic neurons from neurospheres of mesencephalic precursors.
Cultures of rat mesencephalic precursors treated with interleukin-1beta or angiotensin II contained significantly more dopaminergic neurons than controls. However, simultaneous treatment with angiotensin II and interleukin-1beta did not induce any further increase. Treatment with the angiotensin type-2 receptor antagonist PD 123319 precluded both the angiotensin- and the interleukin-induced increase. The present results indicate that angiotensin type-2 receptors and interleukin-1 cooperate to induce the dopaminergic phenotype.
Rodriguez-Pallares
Jannette
J
Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Guerra
Maria J
MJ
Labandeira-Garcia
Jose L
JL
D000704:Analysis of Variance; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D004347:Drug Interactions; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D008636:Mesencephalon / Q000166:cytology*; D009287:Naphthyridines / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D013234:Stem Cells / Q000187:drug effects*; D014404:Tubulin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D007093:Imidazoles; D007375:Interleukin-1; D009287:Naphthyridines; D011725:Pyridines; D014404:Tubulin; C095905:ZD 7155; D000804:Angiotensin II; C073402:PD 123319; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine