Effect of histone H1 on estrogen receptor status of human breast cancer MCF 7 cells.
The effects of exogenous histone H1 on estrogen receptor status of human breast cancer MCF 7 cells were investigated in presence and absence of estrogen. Exogenous histone H1 was significantly cytotoxic in a dose- and time-dependent manner. Cell cycle analysis revealed a significant increase in the percentage of cell accumulation in G0/G1 phase. In histone H1-treated cells, a significant decrease in the estrogen receptor content and an increase in the dissociation constant (KD) of ER was observed compared to control.
Vani
G
G
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India. vangan@yahoo.com
Devi
C S Shyamala
CS
D001943:Breast Neoplasms / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D006657:Histones / Q000494:pharmacology*; D006801:Humans; D011960:Receptors, Estrogen / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D004965:Estrogen Antagonists; D006657:Histones; D011960:Receptors, Estrogen; D004958:Estradiol