Surfaces and orientations: much to FRET about?
Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.
Rasnik
Ivan
I
Physics Department and Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA.
McKinney
Sean A
SA
Ha
Taekjip
T
D001710:Biotin; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008968:Molecular Conformation; D011485:Protein Binding; D011791:Quartz; D012710:Serum Albumin, Bovine; D019809:Streptavidin
D011791:Quartz; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin