title: SARS coronavirus detection methods.
abstract: Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
authors: Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.
authors: Susanna K P
authors: SK
authors: Lau
authors: Xiao-Yan
authors: XY
authors: Che
authors: Patrick C Y
authors: PC
authors: Woo
authors: Beatrice H L
authors: BH
authors: Wong
authors: Vincent C C
authors: VC
authors: Cheng
authors: Gibson K S
authors: GK
authors: Woo
authors: Ivan F N
authors: IF
authors: Hung
authors: Rosana W S
authors: RW
authors: Poon
authors: Kwok-Hung
authors: KH
authors: Chan
authors: J S Malik
authors: JS
authors: Peiris
authors: Kwok-Yung
authors: KY
authors: Yuen
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D006801:Humans; D019590:Nucleocapsid Proteins / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000652:urine
chemical_list: D019590:Nucleocapsid Proteins