title: [Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC].
abstract: OBJECTIVE
  To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.
  
  
  METHODS
  The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm.
  
  
  RESULTS
  The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.
  
  
  CONCLUSIONS
  The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.
authors: Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
authors: Sheng
authors: S
authors: Lin
authors: Qi-Wei
authors: QW
authors: Zhang
authors: Ning-Ning
authors: NN
authors: Zhang
authors: Yong-Xin
authors: YX
authors: Zhang
mesh_terms: D019306:Artemisia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D047309:Flavones / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D047309:Flavones; C039824:cirsiliol; C057618:cirsilineol; C007072:cirsimaritin