Single-step conversion of cephalosporin-C to 7-aminocephalosporanic acid by free and immobilized cells of Pseudomonas diminuta.
7-Aminocephalosporanic acid (7-ACA), the starting material for the production of a number of clinically used semisynthetic cephalosporins, is produced by deacylation of cephalosporin-C. The production of 7-ACA was studied in various modes, at the optimal conditions using free and immobilized whole cells of Pseudomonas diminuta.
Nigam
V K
VK
Birla Institute of Scientific Research, Statue Circle, Jaipur-302001, Rajasthan, India. nigam_vinod@yahoo.com
Kundu
S
S
Ghosh
P
P
D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism*; D002511:Cephalosporins / Q000096:biosynthesis / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D010399:Penicillin Amidase / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature
D002511:Cephalosporins; C030735:7-aminocephalosporanic acid; D010399:Penicillin Amidase