Vitellogenin expression in queen ovaries and in larvae of both sexes of Apis mellifera.
In the honeybee, Apis mellifera, vitellogenin (Vg) expression has been detected in the ovary of queens, but not in that of workers. In addition, larvae of both sexes produce Vg in significant amounts, which suggest that Vg serves for functions additional to oocyte growth and energy supply to the embryo. In vivo hormone treatment experiments suggest that the decrease of 20-hydroxyecdysone concentration occurring in previtellogenic phases allows Vg production. Southern analysis indicates that the Vg gene is present as a single copy in the honeybee genome.
Guidugli
Karina R
KR
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto-SP, Brasil.
Piulachs
Maria-Dolors
MD
Bellés
Xavier
X
Lourenço
Anete P
AP
Simões
Zilá L P
ZL
D000818:Animals; D001516:Bees / Q000378:metabolism*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D018628:Gene Dosage; D007605:Juvenile Hormones / Q000494:pharmacology; D008297:Male; D010053:Ovary / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014819:Vitellogenins / Q000096:biosynthesis* / Q000235:genetics
D007605:Juvenile Hormones; D011725:Pyridines; D014819:Vitellogenins; C055613:pyriproxyfen; D004441:Ecdysterone