Pseudoalteromonas spongiae sp. nov., a novel member of the gamma-Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters.
A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006(T)) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006(T) did not have flagella and were non-motile. Colonies were pale orange in colour, 2-4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006(T) was heterotrophic, strictly aerobic and required NaCl for growth (2.0-6.0%). It grew at pH 5.0-10.0 and between 12 and 44 degrees C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006(T) within the genus Pseudoalteromonas of the gamma-subclass of the Proteobacteria. The DNA G+C content is 40.6 mol% and the dominant fatty acids were 12:0 3-OH, 14:0, 15:0 iso 2-OH, 16:0, 16:1omega7, 17:1omega8 and 18:1omega7 (altogether representing 75.9% of the total). These data supported the affiliation of UST010723-006(T) to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95.4 to 96.8%. UST010723-006(T) differed from these closest relatives by 9-19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006(T) constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006(T) (=NRRL B-41100(T)=JCM 12884(T)).
Lau
Stanley C K
SCK
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Tsoi
Mandy M Y
MMY
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Li
Xiancui
X
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Dobretsov
Sergey
S
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Plakhotnikova
Yulia
Y
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Wong
Po-Keung
PK
Department of Biology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Qian
Pei-Yuan
PY
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D006723:Hong Kong; D008969:Molecular Sequence Data; D010641:Phenotype; D011161:Porifera / Q000382:microbiology*; D031161:Pseudoalteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S