title: Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65).
abstract: The nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria.
authors: Department of Microbiology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Hong
authors: H
authors: Kim
authors: Department of Microbiology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Sun-Hyun
authors: SH
authors: Kim
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-600, Korea.
authors: Tae-Sun
authors: TS
authors: Shim
authors: Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-600, Korea.
authors: Mi-Na
authors: MN
authors: Kim
authors: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Korea.
authors: Gill-Han
authors: GH
authors: Bai
authors: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Korea.
authors: Young-Gil
authors: YG
authors: Park
authors: Department of Microbiology, College of Medicine, Konkuk University, Chungju 380-230, Korea.
authors: Sueng-Hyun
authors: SH
authors: Lee
authors: Institute of Hansen's Disease, The Catholic University Medical College, Seoul 137-7014, Korea.
authors: Gue-Tae
authors: GT
authors: Chae
authors: Department of Microbiology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Chang-Yong
authors: CY
authors: Cha
authors: Department of Microbiology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Yoon-Hoh
authors: YH
authors: Kook
authors: Department of Microbiology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Bum-Joon
authors: BJ
authors: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D018833:Chaperonins