Improvement of xylanase production in solid-state fermentation by alkali tolerant Aspergillus versicolor MKU3.
OBJECTIVE
To optimize the media components for xylanase production by Aspergillus versicolor MKU3 in solid-state fermentation (SSF).


RESULTS
Medium optimization was carried out using De Moe's fractional factorial design with seven components. Maximum production of xylanase 3249.9 U g(-1) was obtained in SSF with an optimized medium containing (g l(-1)): NaNO(3), 20; K(2)HPO(4), 20; MgSO(4), 10; FeSO(4), 0.001; KCl, 1; peptone, 10 and yeast extract, 10. Four components namely NaNO(3), MgSO(4), peptone and K(2)HPO(4) significantly increased the xylanase production by A. versicolor MKU3.


CONCLUSIONS
Fractional factorial design was used to optimize the seven components in the fermentation medium for SSF. The optimized media increased xylanase production by 3.4-fold.


CONCLUSIONS
Aspergillus versicolor MKU3 produced maximum xylanase after two steps of media optimization under alkaline condition. This medium will be significant value for xylanase production in SSF.
Jeya
M
M
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, Madurai, India.
Thiagarajan
S
S
Gunasekaran
P
P
D000468:Alkalies / Q000494:pharmacology; D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D007218:Industrial Microbiology; D014995:Xylosidases / Q000096:biosynthesis*
D000468:Alkalies; D003470:Culture Media; D014995:Xylosidases