title: Plasmid-specified FemABX-like immunity factor in Staphylococcus sciuri DD 4747.
abstract: A plasmid from Staphylococcus sciuri DD 4747 had three open reading frames: a replication gene, an N-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). The epr-like gene was introduced into S. aureus RN4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. Based on both its function and its similarity to femAB, this gene is a member of the femABX-like immunity gene family. Furthermore, this is the first example of a femABX-like immunity gene that is not linked to the gene for the bacteriolytic enzyme against which it specifies immunity.
authors: Department of Biological Sciences, The University of Alabama, Tuscaloosa, AL 35487-0334, USA.
authors: Lucie S
authors: LS
authors: Heath
authors: Shaw R
authors: SR
authors: Gargis
authors: S Rochelle
authors: SR
authors: Smithberg
authors: Heather P
authors: HP
authors: Johnson
authors: Harry E
authors: HE
authors: Heath
authors: Paul A
authors: PA
authors: Leblanc
authors: Gary L
authors: GL
authors: Sloan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D013210:Staphylococcus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C107107:Epr protein, Staphylococcus capitis; D010450:Endopeptidases; C021524:lysostaphin endopeptidase