[Effects of different means of fluid resuscitation on apoptosis of visceral organs in rats with hemorrhagic shock].
OBJECTIVE
To observe the effects of different means of fluid resuscitation on apoptosis of visceral organs in rats with hemorrhagic shock.


METHODS
The tails of 8 male SD rats were cut to cause active bleeding. Blood was collected from the carotid arteries of another 24 male SD rats and heparinized, then the 24 rats were randomly divided into 3 equal groups: no fluid resuscitation group (NF group, carotid blood was collected as described above, the tail was cut and the blood from the tail was collected in container with heparin 30 min after, and hemostasis and heparin blood transfusion were performed 60 min after cutting of the tail), controlled fluid resuscitation group [NS40 group: isosmotic saline was infused during the period of 30 to 60 min after the tail cutting to maintain the mean arterial pressure (MAP) at about 40 mm Hg and then hemostasis and heparin blood transfusion were performed 60 min after cutting of the tail], and great quantity fluid rapid resuscitation group [NS80 group: a great quantity of isosmotic saline was infused during the period of 30 to 60 min after the tail cutting to maintain the MAP at about 80 mm Hg and then hemostasis and heparin blood transfusion were performed 60 min after cutting of the tail]. Blood specimens were collected at the time points 0, 120, and 150 min to undergo blood routine examination. Another blood specimens were collected at the time points 0, 30, 60, and 90 min to undergo lactic acid examination. The surviving rats were killed and their livers, kidneys, lungs, and small intestines were taken out to undergo pathology. Cell apoptosis was examined by flow cytometry and TUNEL.


RESULTS
The survival rates of the NS40 and NS80 groups were significantly higher than that of the NF group (both P < 0.05). The blood lactic acid levels of the NS40 and NS80 groups at the time points 60 and 90 min were all significantly lower than those of the NF and control groups (all P < 0.05). Apoptosis in the liver, kidney, and small intestine mucosa of the NS80 group was significantly marked than in the NF and NS40 groups (all P < 0.01).


CONCLUSIONS
Controlled fluid resuscitation obviously reduces the early death rate of rats with severe hemorrhagic shock and apoptosis in the liver, kidney, and small intestine mucosa thereof and may benefit the prognosis.
Lu
Yuan-qiang
YQ
The Sir Run Run Shaw Hospital Affiliated to Medical College, Zhejiang University, Hangzhou 310016, China.
Cai
Xiu-jun
XJ
Gu
Lin-hui
LH
Wang
Qi
Q
Bao
De-guo
DG
D000818:Animals; D017209:Apoptosis; D005440:Fluid Therapy / Q000379:methods; D007262:Infusions, Intravenous; D007421:Intestine, Small / Q000473:pathology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D011379:Prognosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000473:pathology / Q000628:therapy*