Facile method for the preparation of lyso-GM1 and lyso-GM2.
This paper reports a facile method for the preparation of lyso-GM1 [Gal beta1-->3GalNAc beta1--> 4(Neu5Ac alpha2-->3)Galbeta1-->4Glc beta1-->1'-sphingosine] and lyso-GM2 [GalNAc beta1-->4(Neu5Ac alpha2-->3)Gal beta1-->4Glc beta1-->sphingosine], respectively, from GM1 [Galbeta1-->3GalNAc beta1-->4(Neu5Ac alpha2-->3)Galbeta1-->4Glc beta1-->1'-Cer] and GM2[GalNAc beta1-->4(Neu5Ac alpha2-->3)Galbeta1-->4Glc beta1-->1'-Cer], using sphingolipid ceramide deacylase and high performance anion-exchange chromatography (HPAEC). The enzymatically released lyso-GM1 and/or lyso-GM2 was effectively separated from its parent ganglioside by HPAEC using a Mono Q HR 5/5 column with an Amersham Biosciences fast protein liquid chromatography system. The yield was almost quantitative and the separation completed in approximately 3 h. This method is more convenient and effective than the conventional method using alkaline hydrolysis and silicic acid chromatography to generate and purify lyso-gangliosides.
Ando
Takayuki
T
Department of Biochemistry, Tulane University Health Sciences Center School of Medicine, New Orleans, LA 70112, USA.
Li
Su-Chen
SC
Ito
Makoto
M
Li
Yu-Teh
YT
D000581:Amidohydrolases / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D005678:G(M2) Ganglioside / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism
C029465:lysoGM1 ganglioside; C048941:lysoganglioside G(M2); D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; D000581:Amidohydrolases; C096410:sphingolipid ceramide N-deacylase