[Effects of mitomycin C on the expression and transport of ice-nuclei proteins of Erwinia herbicola].
Abstract: In this paper, Mitomycin C (MMC) was added to different kinds of medium to study the effects of different cultural conditions on the Erwinia herbicola 10025A. For the first time it was confirmed that the expressed activity of the ice-nuclei active protein was different from its transportable manner from the ice nucleation active bacteria (Erwinia herbicola 10025A). The findings indicated that MMC could stimulate the SOS response,and induce the synthesis of some enzymes and proteins, which take part in repairing the damaged DNA. The effects of the MMC on the E. herbicola under different media were different. It could increase the ice nucleation activity of the E. herbicola, forming new small vesicles, which are secreted to the outside of membrane. The importance of this research for study the living mechanism of cells ander poor condition was discussed.
Chen
Qing-Sen
QS
The Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China. chqsen@tjcu.edu.cn
Gao
Xiu-Zhi
XZ
Yan
Ya-Li
YL
Song
Li-Ping
LP
Pang
Guang-Chang
GC
Guo
Shu-Hua
SH
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D004885:Erwinia / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005615:Freezing; D008854:Microscopy, Electron; D016685:Mitomycin / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D013014:SOS Response, Genetics; D013696:Temperature
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; C063113:ice nucleation protein; D016685:Mitomycin