A versatile promoter for the expression of proteins in glandular and non-glandular trichomes from a variety of plants.
A DNA regulatory fragment was isolated from the promoter region of the OASA1 gene, encoding the cytosolic O-acetylserine(thiol)lyase enzyme that is highly expressed in Arabidopsis thaliana trichomes. This DNA fragment has been named an ATP fragment and comprises 1435 bp of the genomic region upstream of the OASA1 gene and 375 bp of the transcriptional initiation start site containing the first intron of the gene. The ATP fragment, fused to the green fluorescent protein (GFP) and beta-glucuronidase (GUS) reporter genes, is able to drive high-level gene expression in A. thaliana trichomes. Deletion analysis of the ATP fragment determined that the region from -266 to -66 contains regulatory elements required for trichome expression. In addition, the region from +112 to +375, comprising the first intronic region of the gene, is also essential for trichome gene expression. Expression of the full-length ATP fragment in tobacco and peppermint shows that this fragment is also able to drive expression in glandular trichomes and suggests additional biotechnological applications for this promoter.
Gutiérrez-Alcalá
Gloria
G
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, CSIC-Universidad de Sevilla, Avda Américo Vespucio, 49, 41092-Sevilla, Spain.
Calo
Leticia
L
Gros
Florence
F
Caissard
Jean-Claude
JC
Gotor
Cecilia
C
Romero
Luis C
LC
D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D036142:Mentha piperita / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014026:Nicotiana / Q000235:genetics
D029681:Arabidopsis Proteins; D010940:Plant Proteins