title: Direct interaction between prion protein and tubulin.
abstract: Recently published data show that the prion protein in its cellular form (PrP(C)) is a component of multimolecular complexes. In this report, zero-length cross-linking with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) allowed us to identify tubulin as one of the molecules interacting with PrP(C) in complexes observed in porcine brain extracts. We found that porcine brain tubulin added to these extracts can be cross-linked with PrP(C). Moreover, we observed that the 34 kDa species identified previously as full-length diglycosylated prion protein co-purifies with tubulin. Cross-linking of PrP(C) species separated by Cu(2+)-loaded immobilized metal affinity chromatography confirmed that only the full-length protein but not the N-terminally truncated form (C1) binds to tubulin. By means of EDC cross-linking and cosedimentation experiments, we also demonstrated a direct interaction of recombinant human PrP (rPrP) with tubulin. The stoichiometry of cosedimentation implies that rPrP molecules are able to bind both the alpha- and beta-isoforms of tubulin composing microtubule. Furthermore, prion protein exhibits higher affinity for microtubules than for unpolymerized tubulin.
authors: Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland. k.nieznanski@nencki.gov.pl
authors: Krzysztof
authors: K
authors: Nieznanski
authors: Hanna
authors: H
authors: Nieznanska
authors: Krzysztof J
authors: KJ
authors: Skowronek
authors: Katarzyna M
authors: KM
authors: Osiecka
authors: Dariusz
authors: D
authors: Stepkowski
mesh_terms: D000818:Animals; D001665:Binding Sites; D006801:Humans; D008970:Molecular Weight; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry; D018621:PrPC Proteins / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D013552:Swine; D014404:Tubulin / Q000032:analysis / Q000737:chemistry*
chemical_list: D046912:Multiprotein Complexes; D018621:PrPC Proteins; D014404:Tubulin