title: Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis.
abstract: Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome.
authors: Laboratory of General Microbiology, University Bremen, P.O. Box 33 04 40, D-28334 Bremen, Germany.
authors: Federico
authors: F
authors: Battistoni
authors: Daniela
authors: D
authors: Bartels
authors: Olaf
authors: O
authors: Kaiser
authors: Stella
authors: S
authors: Marie Reamon-Buettner
authors: Thomas
authors: T
authors: Hurek
authors: Barbara
authors: B
authors: Reinhold-Hurek
mesh_terms: D020627:Azoarcus / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D005822:Genetic Vectors; D016680:Genome, Bacterial; D008970:Molecular Weight; D009876:Operon / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal