Fragmentation of oligosaccharide ions with 157 nm vacuum ultraviolet light.
The 157 nm photofragmentation of native and derivatized oligosaccharides was studied in a linear ion trap and in a home-built matrix-assisted laser desorption/ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometer, and the results were compared with collision-induced dissociation (CID) experiments. Photodissociation produces product ions corresponding to high-energy fragmentation pathways; for cation-derivatized oligosaccharides, it yields strong cross-ring fragment ions and provides better sequence coverage than low- and high-energy CID experiments. On the other hand, for native oligosaccharides, CID yielded somewhat better sequence coverage than photodissociation. The ion trap enables CID hybrid MS3 experiments on the high-energy fragment ions obtained from photodissociation.
Devakumar
Arugadoss
A
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Thompson
Matthew S
MS
Reilly
James P
JP
D002240:Carbohydrate Sequence; D019791:Guanidine / Q000737:chemistry; D007477:Ions / Q000737:chemistry / Q000528:radiation effects; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000528:radiation effects*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014466:Ultraviolet Rays
D007477:Ions; D009844:Oligosaccharides; D019791:Guanidine