title: The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori.
abstract: As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.
authors: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea. hwangjs@rda.go.kr
authors: Jae-Sam
authors: JS
authors: Hwang
authors: Hyun-Jeong
authors: HJ
authors: Go
authors: Tae-Won
authors: TW
authors: Goo
authors: Eun-Young
authors: EY
authors: Yun
authors: Kwang-Ho
authors: KH
authors: Choi
authors: Su-Il
authors: SI
authors: Seong
authors: Sang-Mong
authors: SM
authors: Lee
authors: Bong-Hee
authors: BH
authors: Lee
authors: Iksoo
authors: I
authors: Kim
authors: Taehoon
authors: T
authors: Chun
authors: Seok-Woo
authors: SW
authors: Kang
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D035163:Bombax / Q000196:embryology* / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D003576:Electron Transport Complex IV