title: Genetic basis for dissemination of armA.
abstract: OBJECTIVE
  armA is a novel plasmid-borne 16S rRNA methyltransferase that confers high-level resistance to 4,6-disubstituted deoxystreptamines. Recently, we have isolated from a high-level broad-spectrum aminoglycoside-resistant Escherichia coli animal isolate a plasmid, pMUR050, that bore the armA gene. In order to elucidate the genetic basis for the spread of armA, we have determined the complete nucleotide sequence of pMUR050.
  
  
  RESULTS
  armA was borne by a complex transposon composite flanked by two direct repeats of IS26. The transposon composite included a class one integron with sul1 for resistance to sulphonamides and ant3''9 conferring resistance to spectinomycin-streptomycin, and a macrolide efflux pump and mefE/mel conferring high-level resistance to erythromycin. We identified in GenBank that another plasmid, pCTX-M3, from a Polish Citrobacter freundii human isolate, bore the same genetic structure, including armA.
  
  
  CONCLUSIONS
  armA is present in human and animal isolates within a novel transposon composite. Further spread of armA between bacteria of diverse origin is to be expected.
authors: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain. bgzorn@vet.ucm.es
authors: Bruno
authors: B
authors: González-Zorn
authors: Ana
authors: A
authors: Catalan
authors: Jose A
authors: JA
authors: Escudero
authors: Lucas
authors: L
authors: Domínguez
authors: Tirushet
authors: T
authors: Teshager
authors: Concepción
authors: C
authors: Porrero
authors: Miguel Angel
authors: MA
authors: Moreno
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D023061:Gene Order; D006801:Humans; D032023:Integrons; D026901:Membrane Transport Proteins / Q000235:genetics; D008780:Methyltransferases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D017421:Sequence Analysis
chemical_list: D000617:Aminoglycosides; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D012336:RNA, Ribosomal, 16S; C506767:ArmA protein, E coli; D008780:Methyltransferases