title: Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus.
abstract: A halotolerant, obligately alkaliphilic bacterium, R-2(T), was isolated from the skin of a rainbow trout (Oncorhynchus mykiss), a freshwater fish. The strain is Gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. The isolate grows at pH 9-10 but not at pH 7-8. This micro-organism grows in 0-22% (w/v) NaCl at pH 10. Its major cellular fatty acids are iso-C(15:0), anteiso-C(15:0) and anteiso-C(17:0), the major isoprenoid quinone is MK-7 and the DNA G+C content is 38.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain R-2(T) is a member of the genus Oceanobacillus. DNA-DNA hybridization reveals low relatedness between the isolate and Oceanobacillus iheyensis (21.0%). On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate should be designated as a novel species, for which the name Oceanobacillus oncorhynchi sp. nov. is proposed. The type strain is R-2(T) (=JCM 12661(T)=NCIMB 14022(T)).
authors: Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors: Isao
authors: I
authors: Yumoto
authors: Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors: Kikue
authors: K
authors: Hirota
authors: Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
authors: Yoshinobu
authors: Y
authors: Nodasaka
authors: Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors: Kenji
authors: K
authors: Nakajima
mesh_terms: D000818:Animals; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*; D012965:Sodium Chloride; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride