Four new C-21 steroidal glycosides from the roots of Stephanotis mucronata and their immunological activities.
Four new C-21 steroidal glycoside, stemucronatosides D (1), E (2), F (3), and G (4) were isolated from the roots of Stephanotis mucronata. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods including one-dimensional and two-dimensional NMR. These isolated compounds were assayed for their immunological activities in vitro against concanavalin A (Con A)- and lipopolysaccharide (LPS)-induced proliferation of mice splenocytes. Compounds 1, 2, and 4 showed immunosuppressive activities in a dose-dependent manner, while compound 3 showed immunomodulating activities.
Ye
Yiping
Y
Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
Sun
Hongxiang
H
Li
Xiaoyu
X
Chen
Fengyang
F
Qin
Feng
F
Pan
Yuanjian
Y
D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000502:physiology
D007155:Immunologic Factors; D008070:Lipopolysaccharides; D008934:Mitogens; D012503:Saponins; D003208:Concanavalin A