Effect of different zinc sources and levels on inhibition of the apoptosis induced by glucocorticoid of thymocytes in vitro.
This experiment was conducted to investigate the effects of zinc sulfate and zinc methionine (Zn-Met) and their levels on apoptosis induced by glucocorticoid of thymocytes and the possible mechanism. Dexamethasone was used to make the apoptosis model of thymocytes; zinc sulfate and zinc methionine were supplemented to the medium at levels of 0, 50, 100, 500, and 1000 microM. The activity of cells, Cu,Zn superoxide dismutase (Cu,Zn-SOD), DNA ladder pattern, intracellular calcium concentration, and the percentage of apoptosis nuclei were determined. Both ZnSO4 and Zn-Met could modulate apoptosis; they inhibited apoptosis and decreased DNA fragmentation. The regulation was concentration dependent. At levels of 50 and 100 microM, the effect of Zn-Met on inhibiting apoptosis was less efficient than that of ZnSO4 (p<0.05), but the activity of the cells cultured with Zn-Met was higher than those cultured with ZnSO4; they showed no difference in modulating apoptosis when added at levels of 500 and 1000 microM to the medium (p>0.05). Intracellular calcium concentrations of cells cultured with Zn-Met were higher than those cultured with ZnSO4 at the same levels. Zinc supplementation decreased the concentration of intracellular calcium significantly (p<0.05) and increased the activity of Cu,Zn-SOD in the extract of the cells (p<0.05). Both zinc sulfate and Zn-Met could modulate apoptosis of thymocytes induced by glucocorticoid; the mechanism might involve the exchange of intracellular calcium, the redox of cells, and the two forms of zinc might go different ways in the regulations.
Ze-peng
Yu
Y
Department of Animal Nutrition, School of Food Science and Technology, Southern Yangtze University, Wuxi, Jiangsu Province, People's Republic of China.
Guo-wei
Le
L
Hong-yu
Huang
H
Yun-yu
Wei
W
Yong-hui
Shi
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D053938:DNA Fragmentation; D003907:Dexamethasone / Q000494:pharmacology; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*; D017967:Zinc Compounds / Q000378:metabolism; D019287:Zinc Sulfate / Q000378:metabolism
D000975:Antioxidants; D004396:Coloring Agents; D005938:Glucocorticoids; D013778:Tetrazolium Salts; D013844:Thiazoles; D017967:Zinc Compounds; D019287:Zinc Sulfate; D003907:Dexamethasone; D004247:DNA; D013482:Superoxide Dismutase; C022616:thiazolyl blue; D015032:Zinc; D002118:Calcium