Lectin arrays for profiling cell surface carbohydrate expression.
We present a strategy for the analysis of cell surface carbohydrate expression patterns using lectin arrays fabricated on gold surfaces. Antibody and glycoprotein binding experiments showed that the lectins were effectively immobilized on the surface and retained their carbohydrate-binding specificities. The approach was demonstrated in the analysis of carbohydrate expression on two mammalian cell lines.
Zheng
Ting
T
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706-1396, USA.
Peelen
Dora
D
Smith
Lloyd M
LM
D000818:Animals; D000954:Antigens, Surface / Q000737:chemistry / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D002477:Cells / Q000166:cytology; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D013379:Substrate Specificity; D013499:Surface Properties
D000954:Antigens, Surface; D002241:Carbohydrates; D037102:Lectins