[Multiplex amplification test system for the identification and differentiation of Bacillus anthracis].
The multiplex amplification test system for the identification of Bacillus anthracis with primers to plasmid cya (pX01), capC (pX02) genes and chromosomal sap gene were developed. The primers to sap gene were selected by the authors and, after being tested on 72 microbial strains of the genus Bacillus, proposed as more specific in comparison with the known primers to chromosomal locus Ba 813. The proposed test system permitted the simultaneous identification of B. anthracis of all plasmid variants, the evaluation of their potential virulence and the differentiation of B. anthracis nonplasmid strains from bacilli of the group Bacillus cereus.
Tsygankova
O I
OI
Eremenko
E I
EI
Riazanova
A G
AG
Tsygankova
E A
EA
D000942:Antigens, Bacterial / Q000235:genetics; D001408:Bacillus anthracis / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D008562:Membrane Glycoproteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C030325:anthrax toxin