Pathology-guided MR analysis of acute and chronic experimental allergic encephalomyelitis spinal cord lesions at 1.5T.
OBJECTIVE
To directly correlate spinal cord pathology of guinea pigs with experimental allergic encephalomyelitis (EAE) to the MRI data obtained at 1.5T.


METHODS
Spinal cords from EAE animals were imaged in vivo with the following MRI sequences: T2-FSE, PD-FSE, fluid-attenuated inversion recovery (FLAIR)-FSE, T2-CSE, T1-CSE, T1-CSE + gadolinium-DTPA (Gd-DTPA), PD-CSE, and short-tau inversion recovery (STIR)-FSE. The spinal cords were removed and the lesions with specific pathological compositions were identified by histological analysis. Regions of interest (ROIs) were drawn on the corresponding MR images, and signal-to-noise ratios (SNRs) were measured for each MR sequence and compared with controls.


RESULTS
The receiver operating characteristic (ROC) analysis of STIR-FSE and PD-CSE was able to differentiate tissue that contained cellular infiltrates with a high degree of accuracy. The SNRs of T2-FSE, STIR-FSE, T2-CSE, PD-CSE, and T1-CSE + Gd-DTPA were elevated in lesions that contained cellular infiltrates alone, whereas the SNRs of PD-CSE and T1-CSE + Gd-DTPA were reduced in demyelinated lesions that also contained inflammation.


CONCLUSIONS
The SNR difference between the two lesion groups suggests that the combination of STIR-FSE, PD-CSE, and T1-CSE + Gd-DTPA sequences may be useful for differentiating inflammatory lesions containing demyelination from lesions with inflammation alone.
Cook
Lisa L
LL
Department of Physiology and Pharmacology, University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 5C1, Canada.
Foster
Paula J
PJ
Karlik
Stephen J
SJ
D000208:Acute Disease; D000818:Animals; D019540:Area Under Curve; D001707:Biopsy, Needle; D001921:Brain / Q000473:pathology*; D002908:Chronic Disease; D003287:Contrast Media; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000175:diagnosis* / Q000473:pathology; D019786:Gadolinium DTPA; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D011336:Probability; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000175:diagnosis* / Q000473:pathology
D003287:Contrast Media; D019786:Gadolinium DTPA