Physical, mechanical and degradation properties, and schwann cell affinity of cross-linked chitosan films.
Three kinds of cross-linked chitosan films were prepared with hexamethylene diisocyanate (HDI), epichlorohydrin (ECH) and glutaraldehyde (GA) as cross-linking agents, respectively. The physical and mechanical properties, biodegradability and Schwann cell affinity of the cross-linked films were investigated. A significant decrease in the degradation rate in lysozyme solution and a large change in the mechanical properties were observed compared with non-cross-linked chitosan films. The protein adsorption on chitosan films was determined by means of enzyme-linked immunosorbent assay (ELISA). In comparison with the non-cross-linked films, the chitosan films cross-linked with HDI showed a significant increase (up to 40-50%) in both fibronectin and laminin adsorption, while the protein adsorption on the other two kinds of cross-linked films was similar to that on non-crosslinked films. In addition, cell culture revealed that the HDI cross-linked chitosan films enhanced the spread and proliferation of Schwann cells while the other cross-linked films delayed the cell proliferation. These results suggest that HDI cross-linking of chitosan films provides a combination of physical properties, biodegradability and Schwann cell affinity suitable for peripheral nerve regeneration.
Cao
Wenling
W
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, P R. China.
Cheng
Mingyu
M
Ao
Qiang
Q
Gong
Yandao
Y
Zhao
Nanming
N
Zhang
Xiufang
X
D000327:Adsorption; D000818:Animals; D001673:Biodegradation, Environmental; D049109:Cell Proliferation; D048430:Cell Shape; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003432:Cross-Linking Reagents; D005353:Fibronectins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008297:Male; D019563:Mechanics; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism*
D003432:Cross-Linking Reagents; D005353:Fibronectins; D007797:Laminin; D008565:Membrane Proteins; D048271:Chitosan