Separation and determination of four active anthraquinones in Chinese herbal preparations by flow injection-capillary electrophoresis.
A simple, rapid, and accurate method for the separation and determination of physcion, chrysophanol, aloe-emodin, and emodin in Rhubarb, Juemingzi, and Chinese herbal preparations was developed by combination of flow injection-capillary zone electrophoresis for the first time. The analysis was carried out using an unmodified fused-silica capillary (75 mm x 50 microm ID x 375 microm OD, effective separation length of 48 mm) and direct ultraviolet detection at 254 nm. By a series of optimization, the sample solvent consisted of NaOH (100 mmol/L) and ACN (1:1 v/v), and a running buffer composed of 15 mmol/L sodium borate - 12.5 mmol/L sodium dihydrogen phosphate - 42% v/v ACN (pH 10.1) was applied for the separation of the four anthraquinones. The separation was rapid and highly reproducible, with complete resolution of all four compounds within 6 min. The sample throughput rate could reach up to 12 per h. The repeatability (defined as relative standard deviation) was 4.45, 4.44, 4.34, 0.61% with peak height evaluation and 1.62, 0.89, 2.49, 2.19% with peak area evaluation for physcion, chrysophanol, aloe-emodin, and emodin, respectively.
Liu
Lihong
L
Department of Chemistry, Lanzhou University, P.R. China.
Fan
Liuyin
L
Chen
Hongli
H
Chen
Xingguo
X
Hu
Zhide
Z
D000880:Anthraquinones / Q000032:analysis*; D002021:Buffers; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019075:Electrophoresis, Capillary; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis; D017022:Flow Injection Analysis; D006863:Hydrogen-Ion Concentration; D012250:Rheum / Q000737:chemistry
D000880:Anthraquinones; D002021:Buffers; D004365:Drugs, Chinese Herbal; C518327:aloe emodin; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid