High-performance liquid chromatography-mass spectrometry method for determination of anagrelide in human plasma.
This paper describes a simple, fast and sensitive liquid chromatography-mass spectrometry method for quantification of an anti-thrombocythemic agent, anagrelide in human plasma. The samples were subjected to a liquid-liquid extraction after addition of a buffer and an internal standard. Chromatography was performed on an Inertsil ODS2 column and the extract was injected onto a HPLC system coupled with mass spectrometric detection. Linear responses for standards were observed from 50 to 7500 pg/ml. The accuracy of intra-assay and inter-assay were in the ranges 4.3-4.4% and 4.8-5.6%, respectively. The method is simple and reproducible with a run time of less than 2 min.
Zhu
Z
Z
Pharmascience, 6111 Royalmount Ave. Suite 100, Montreal, Que., Canada. zzhu@pharmascience.com
Gonthier
R
R
Neirinck
L
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005343:Fibrinolytic Agents / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011799:Quinazolines / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005343:Fibrinolytic Agents; D011799:Quinazolines; C021139:anagrelide