[2-14C]Thymidine incorporation activity of stem cells in either tumor or cradle tissues in a normal or transplanted animals.
A novel autoradiographic procedure was developed for such continuously cycling cells as stem cells on account of proliferating rate of which is astronomically high per min. Negative visualization is observed over any mitotic image by use of a biomarker, "[2-14C]thymidine" for a few minutes in both cases, either in vivo or in vitro systems. But, good visualization images were realized by many 14C-beta tracks over stem cells with a few minute labelling of [2-14C]thymidine in originated cradles as predicted by Burkitt, H.G(1993). It is clearly elucidated that a short and quick labelling procedure of [2-14C]thymidine is useful to evaluate toxicity and efficacy of new drug candidates and to diagnose cluster of unknown malignity or proliferation rate of respective stem cell in in vivo or Ex-vivo system. Results show that the cell proliferation rate of the stem cells in respective tissues was markedly suppressed, dependent on time after dosing and the dose of 90Y; 3.7, 37, 370, 3,700, and 37,000 kBq per mouse (25g). In addition to the above, the sensitivity of the proliferation rate was dependent on amitosis or mitosis and the AUC value of 90Y-concentration at specific locations of the cells in the mouse body. The most sensitive cells were the plasmacytoma cells, followed by the pluripotent and unipotent stem cells, the intestinal crypts, epiphysial growth plate and liver cells. Results in this presentation, also gives a clear evidence showing a revival of facultative divider line from G0 stage of epithelium and mascular meditate into the unipotent stem cell cycle. Application of [2-14C]thymidine is useful for evaluation of a grade of maturation in differentiation of malignant cells or replicable unipotent stem cells.
Shigematsu
Akiyo
A
Institute of Whole Body Metabolism, Nauchi, Shiroi City, Chiba, Japan. iwbm@d9.dion.ne.jp
Yui
Joji
J
Hamai
Yuko
Y
Hatori
Akiko
A
D000818:Animals; D001345:Autoradiography; D001707:Biopsy, Needle; D002250:Carbon Radioisotopes; D049109:Cell Proliferation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000166:cytology*; D014411:Neoplastic Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D013936:Thymidine