[Experimental evaluation of the bacteriotoxic phototherapy of inflammatory diseases of the oral cavity].
The combined use of laser radiation and Radachlorine revealed the presence of correlation between the number of viable Streptococcus pyogenes cells and the concentration of photosensitizer (r=-0.62), time of irradiation (r=-0.75), as well as energy density (r=-0.80). The death of 100% of suspended S. pyogenes cells was observed at the concentration of Radachlorine reaching 14.4 mM and radiation power reaching 1 W, irrespective of energy density. Pulse and superpulse laser radiation, carried out simultaneously with the use of Radachlorine at a concentration of 1.8 microM, resulted in the death of 100% of S. pyogenes cells in vitro. On the experimental model of inflammation the application of 0.1% Radachlorine gel demonstrated higher effectiveness (in comparison with 0.35% Radachlorine solution) when used simultaneously with laser radiation, both continuous and superpulse (with energy density being 400 j/cm2 and power being 1 W). The use of these parameters in the treatment of the experimental foci of inflammation in the animals led to the complete elimination of S. pyogenes from infected tissues.
Risovannaia
O N
ON
D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D007834:Lasers; D008826:Microbial Sensitivity Tests; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011166:Porphyrins / Q000627:therapeutic use*; D013280:Stomatitis / Q000188:drug therapy* / Q000382:microbiology; D013290:Streptococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000528:radiation effects
D017319:Photosensitizing Agents; D011166:Porphyrins