Importance of quantitative PCR primer location for short interfering RNA efficacy determination.
Shepard
Allan R
AR
Glaucoma Research, Alcon Research Ltd., Fort Worth, TX 76134, USA. allan.shepard@alconlabs.com
Jacobson
Nasreen
N
Clark
Abbot F
AF
D002460:Cell Line; D055513:Connective Tissue Growth Factor; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics*; D034741:RNA, Small Interfering / Q000032:analysis*; D014129:Trabecular Meshwork / Q000737:chemistry; D014162:Transfection
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; C034477:RNA primers; D034741:RNA, Small Interfering; D055513:Connective Tissue Growth Factor; D012313:RNA