Effects of surface undulations of biphasic calcium phosphate tablets on human osteoblast behavior.
In this work, the in vitro behavior of human osteoblast cells on the undulated surfaces of biphasic calcium phosphate tablets was investigated. The tablets were produced by uniaxial pressing with convex cylindrical undulations occupying only half of the surface area; the other half was flat. Chemical and physical characterization was performed by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). XRD and FTIR analyses revealed the presence of hydroxyapatite (HA) and alpha-tricalcium phosphate (alpha-TCP) in a well-defined ratio. Moreover, microtopography, evaluated by SEM and AFM, was similar on the flat region and on that with undulations. However, surface undulations induced different cellular arrangements, confirming the influence of the macrotopography on the cells orientation.
dos Santos
Euler A
EA
Dep. de Eng. Metal. e de Materiais, UFRJ, P.O.Box 68505, Rio de Janeiro, 21941-972, RJ, Brasil.
Linhares
Adriana B R
AB
Rossi
Alexandre M
AM
Farina
Marcos
M
Soares
Gloria A
GA
D001672:Biocompatible Materials / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties; D013607:Tablets / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D013607:Tablets; C020243:calcium phosphate