title: A modified cell culture method for autologous chondrocyte transplantation.
abstract: Autologous chondrocyte transplantation (ACT) is a promising method to treat chondral and osteochondral defects. This study introduced a modified method for cell culture in ACT. Porcine chondrocytes were cultured for 3 weeks under low hydrostatic pressure at 250 Pa. The results showed that the dry weight of the cartilage-like membrane in the loading group was 3.0 times more than the control group (no loading) (p < 0.01), and cell numbers were significantly increased by 3.1 times (p < 0.01) after a 3-week culture. Compared with the fresh tissue sample, the mRNA expression of collagen II was not statistically different and the mRNA of aggrecan was only slightly decreased by 19%. These data suggest that the hydrostatic pressure at this level significantly increased the cell numbers and biosynthesis of cultured chondrocytes.
authors: Department of Orthopaedic Surgery, China Medical University Hospital, Taichung, Taiwan.
authors: Chun-Yi
authors: CY
authors: Lee
authors: Xuhui
authors: X
authors: Liu
authors: Horng-Chaung
authors: HC
authors: Hsu
authors: Der-Yean
authors: DY
authors: Wang
authors: Zong-Ping
authors: ZP
authors: Luo
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D019902:Chondrocytes / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D024043:Collagen Type II / Q000096:biosynthesis; D006874:Hydrostatic Pressure; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D014182:Transplantation, Autologous
chemical_list: D024043:Collagen Type II; D012333:RNA, Messenger