Diabetic ketoacidosis increases extracellular levels of the major inducible 70-kDa heat shock protein.
OBJECTIVE
Diabetic ketoacidosis (DKA) represents a metabolic stress whose treatment induces a systemic proinflammatory cytokine profile and accentuates life-threatening acute complications. The present study determined whether serum levels of the major inducible 70-kDa heat shock protein (Hsp72), a modulator of cytokine expression, were influenced by DKA and its treatment.


METHODS
Serum levels of Hsp72 and glucose were measured in five adolescents with type 1 diabetes mellitus (T1DM) prior to, during and following correction of severe DKA. Samples from nine relatively euglycemic T1DM patients served as controls.


RESULTS
DKA pre-treatment samples showed significant elevation in Hsp72 (40.8 +/- 6.9 ng/ml) relative to euglycemic T1DM controls (33.6 +/- 3.2 ng/ml) (P < 0.05). Treatment resulted in a decline in Hsp72 to control levels within 24 h, with Hsp72 and glucose levels being tightly correlated (r = 0.9258).


CONCLUSIONS
Extracellular Hsp72 is increased by DKA, paralleling changes in serum glucose levels.
Oglesbee
Michael J
MJ
Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA. oglesbee.1@osu.edu
Herdman
Anne V
AV
Passmore
Gregory G
GG
Hoffman
William H
WH
D000293:Adolescent; D001786:Blood Glucose / Q000378:metabolism; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D016883:Diabetic Ketoacidosis / Q000097:blood* / Q000628:therapy; D005260:Female; D005440:Fluid Therapy; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000627:therapeutic use; D008297:Male
D001786:Blood Glucose; D018840:HSP70 Heat-Shock Proteins; D007328:Insulin