Polymorphism of the p53 tumor suppressor gene is associated with susceptibility to uterine leiomyoma.
OBJECTIVE
To evaluate the association between the presence of uterine leiomyoma and two single nuclear polymorphisms of the p53 tumor suppressor and the angiopoietin-2 (ANGPT2) genes.


METHODS
Prospective case control study.


METHODS
Academic research institution.


METHODS
One hundred thirty-two women with clinically and surgically diagnosed uterine leiomyomas and 280 controls.


METHODS
Peripheral venous puncture.


METHODS
Genotyping was performed by polymerase chain reaction-based amplification of the Arg and Pro variants at codon 72 of the p53 gene and by restriction fragment length polymorphism analysis of the G/G and G/A alleles in exon 4 of the ANGPT2 gene.


RESULTS
Comparing women with uterine leiomyomas and controls, no statistically significant difference with respect to allele frequency and genotype distribution were ascertained for the ANGPT2 polymorphism (P=.2 and P=.5, respectively). However, for the p53 tumor suppressor gene polymorphism, statistically significant differences in terms of a higher Pro allele frequency and a higher prevalence of the Pro/Pro genotype among women with uterine leiomyoma (32.0% vs. 16.0%, respectively, and 21.3% vs. 4.7%, respectively) were ascertained (P=.001, OR 1.74; 95% CI 1.24-2.45, P=.001; OR 3.84, 95% CI 1.81-8.14; respectively).


CONCLUSIONS
Carriage of the p53 polymorphism at codon 72 predicts the susceptibility to leiomyoma in a Caucasian population and may contribute to the pathogenesis of uterine leiomyoma.
Denschlag
Dominik
D
Department of Obstetrics and Gynecology, University of Freiburg School of Medicine, Freiburg, Germany. ddenschl@frk.ukl.uni-freiburg.de
Bettendorf
Herta
H
Watermann
Dirk
D
Keck
Christoph
C
Tempfer
Clemens
C
Pietrowski
Detlef
D
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D016158:Genes, p53 / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics*
D016159:Tumor Suppressor Protein p53