title: Human sperm centrosomal function during fertilization, a novel assessment for male sterility.
abstract: In human fertilization, the sperm introduces the centrosome-the microtubule organizing center-and microtubules are organized within the inseminated egg from the sperm centrosome. These microtubules form a radial array, the sperm aster, the functioning of which is essential for pronuclear movement for the union of the male and female genomes. We established functional assay for human sperm centrosomal function, by using heterologus ICSI system with bovine and rabbit eggs. After human sperm incorporation into mammalian egg, we observed that the sperm aster was organized from sperm centrosome, and the sperm aster enlarged as the sperm nuclei underwent pronuclear formation. The normal human sperm aster formation rate at 6 h post-ICSI were 60.0% in bovine egg and 36.1% in rabbit egg, respectively. However, sperm aster formation rate following heterologus ICSI into bovine eggs with teratozoospermia (globozoospermia, dysplasia of fibrous sheath) were low. These data indicate that human sperm centrosomal function is low in abnormal shaped sperm. Wherus, elucidation of human sperm centrosomal function can lead us to find a new type of failure in "post ICSI events in fertilization".
authors: Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Miyagi, Japan. terada@mail.tains.tohoku.ac.jp
authors: Yukihiro
authors: Y
authors: Terada
mesh_terms: D000818:Animals; D002417:Cattle; D018385:Centrosome / Q000502:physiology*; D003599:Cytoskeleton; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000628:therapy; D008297:Male; D010063:Ovum / Q000166:cytology / Q000502:physiology; D011817:Rabbits; D027724:Reproductive Techniques, Assisted; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013094:Spermatozoa / Q000002:abnormalities / Q000166:cytology* / Q000502:physiology*