title: Ultrafine carbon black particles cause early airway inflammation and have adjuvant activity in a mouse allergic airway disease model.
abstract: To gain more insight into the mechanisms of particulate matter (PM)-induced adjuvant activity, we studied the kinetics of airway toxicity/inflammation and allergic sensitization to ovalbumin (OVA) in response to ultrafine carbon black particles (CBP). Mice were exposed intranasally to OVA alone or in combination with different concentrations of CBP. Airway toxicity and inflammation were assessed at days 4 and 8. Immune adjuvant effects were studied in the lung draining peribronchial lymph nodes (PBLN) at day 8. Antigen-specific IgE was measured at days 21 and 28, whereas allergic airway inflammation was studied after OVA challenges (day 28). Results show that a total dose of 200 microg CBP per mouse, but not 20 microg or 2 microg, induced immediate airway inflammation. This 200 microg CBP was the only dose that had immune adjuvant activity, by inducing enlargement of the PBLN and increasing OVA-specific production of Th2 cytokines (IL-4, IL-5, and IL-10). The immune adjuvant activity of 200 microg CBP dosing was further examined. Whereas increased OVA-specific IgE levels in serum on day 21 confirms systemic sensitization, this was further supported by allergic airway inflammation after challenges with OVA. Our data show a link between early airway toxicity and adjuvant effects of CBP. In addition, results indicate that local cytokine production early after exposure to CBP is predictive of allergic airway inflammation. In addition this model appears suitable for studying the role of airway toxicity, inflammation and other mechanisms of particle adjuvant activity, and predicting the adjuvant potential of different particles.
authors: Department of Immunotoxicology, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands. C.deHaar@iras.uu.nl
authors: Colin
authors: C
authors: de Haar
authors: Ine
authors: I
authors: Hassing
authors: Marianne
authors: M
authors: Bol
authors: Rob
authors: R
authors: Bleumink
authors: Raymond
authors: R
authors: Pieters
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002244:Carbon / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000473:pathology; D012140:Respiratory Tract Diseases / Q000139:chemically induced* / Q000473:pathology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D007073:Immunoglobulin E; D002244:Carbon; D010047:Ovalbumin