Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps.
CONCLUSIONS
Our results indicate that the electrogenic kidney Na(+)/HCO(3)(-) cotransporter (kNBC), KCl cotransporter (KCC1 and -4) and Ca(2+)-activated Cl(-) channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells.


OBJECTIVE
Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. Transepithelial transport of ions and water in various fluid-transporting epithelia is strictly dependent on the localization of specific membrane proteins in the polarized epithelial cells. In this study we investigated the expression and distribution of mRNA transcripts for kNBC, pancreatic NBC, KCC1, -2, -3, -4 and CaCC1, -2, -3 gene families in human nasal mucosa and nasal polyp.


METHODS
The expression and localization of these gene families were investigated in inferior turbinate tissues and nasal polyp using reverse transcriptase polymerase chain reaction (RT-PCR), semiquantitative RT-PCR and in situ hybridization.


RESULTS
mRNAs for kNBC, KCC1 and -4 and all the CaCC families (CaCC1, -2 and -3) are expressed in human turbinate mucosa and nasal polyp. The expression levels of kNBC and KCC1 and -4 mRNAs did not differ between nasal mucosa and nasal polyp. However, the expression levels of all the CaCC genes were significantly decreased in nasal polyp. In situ hybridization revealed that the expression of these genes was mainly localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyp.
Lee
Sang Hag
SH
Department of Otorhinolaryngology-Head & Neck Surgery, Colleges of Medicine, Korea University, Seoul, South Korea. sanghag@kumc.or.kr
Park
Ji Hoon
JH
Jung
Hak Hyun
HH
Lee
Seung Hoon
SH
Oh
Joon Whan
JW
Lee
Heung Man
HM
Jun
Hyun Soo
HS
Cho
Woo Jin
WJ
Lee
Jae Yong
JY
D000328:Adult; D001483:Base Sequence; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D017136:Ion Transport / Q000235:genetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism*; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027982:Sodium-Bicarbonate Symporters / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D014420:Turbinates / Q000737:chemistry; D000096922:K Cl- Cotransporters
D018118:Chloride Channels; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D027982:Sodium-Bicarbonate Symporters; D027981:Symporters; D000096922:K Cl- Cotransporters; C119651:SLC12A7 protein, human