Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus.
We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.
Abe
Takumi
T
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Hokkaido, Japan.
Miyake
Norifumi
N
Nishijima
Yasuyuki
Y
Fujita
Ryousuke
R
Sahara
Ken
K
Asano
Shin-Ichiro
S
Bando
Hisanori
H
D000818:Animals; D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics*; D002478:Cells, Cultured; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D018411:Spodoptera / Q000821:virology*; D014158:Transcription, Genetic
D008156:Luciferases; D012321:DNA-Directed RNA Polymerases