title: Novel patterns of the gene expression regulation in the prestalk region along the antero-posterior axis during multicellular development of Dictyostelium.
abstract: Simultaneous hybridization with differentially labeled fluorescent probes for in situ hybridization analysis revealed several novel expression patterns of prestalk genes during multicellular development of Dictyostelium. Seven prestalk genes and one prespore gene (pspA) were analyzed in this study. The patterns identified here indicate that prestalk cells are more heterogeneous than previously thought. Heterogeneity was observed in peripheral prestalk tissues such as the pstAO domain of a slug and the prestalk region surrounding a stalk tube of a culminant. Heterogeneity was also observed in the core pstAB cells of the slug and immature stalk cells within the stalk tube. The upper- and lower-cups of a late culminant were also composed of several subdomains.
authors: Biological Science, Graduate School of Science, Osaka University, Machikaneyama 1-1, Toyonaka, Osaka 560-0043, Japan.
authors: Yoko
authors: Y
authors: Yamada
authors: Haruyo
authors: H
authors: Sakamoto
authors: Satoshi
authors: S
authors: Ogihara
authors: Mineko
authors: M
authors: Maeda
mesh_terms: D000818:Animals; D004023:Dictyostelium / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D017125:Genes, Protozoan / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger