Effect of cholesterol and temperature on the elastic properties of niosomal membranes.
The mechanical characteristics of non-ionic bilayer membranes composed of sorbitan monostearate, cholesterol and poly-24-oxyethylene cholesteryl were studied by measuring the modulus of surface elasticity (mu), a measure of membrane strength, as a function of cholesterol content and temperature. The modulus of surface elasticity increased slowly with increasing cholesterol concentration, with a sharp increase around 40 mol% cholesterol (on average an increment of 0.43x10(6) Nm(-2) per molar percentage), and displayed a maximum of 6.5x10(6) Nm(-2) around 47.5 mol% cholesterol. Further cholesterol resulted in a decrease in mu. Generally the interaction of cholesterol with sorbitan monostearate should increase the rigidity of the membrane. However, the latter effect may be due to the formation of cholesterol clusters at high cholesterol content where excess amounts of cholesterol cannot interact with the sorbitan monostearate, and deposits on the bilayers compromising their uniformity, strength and permeability. This behavior was evident when measurements were carried out above and below 25 degrees C.
Nasseri
Behrooz
B
Centre for Drug Delivery Research, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK. behrooz.nasseri@ulsop.ac.uk
D002784:Cholesterol / Q000737:chemistry*; D004337:Drug Carriers; D004548:Elasticity; D006601:Hexoses; D006860:Hydrogen Bonding; D008051:Lipid Bilayers / Q000737:chemistry*; D013696:Temperature
D004337:Drug Carriers; D006601:Hexoses; D008051:Lipid Bilayers; D002784:Cholesterol; C009298:sorbitan monostearate