High performance liquid chromatography-mass spectrometry based chemometric characterization of olive oils.
In this study the effective discrimination of extra virgin olive oils is described using HPLC-MS, combined with chemometric evaluation. The presented method is simple since the diluted oil sample is directly injected into the system, without any preliminary chemical derivatization or purification step. Separation of diacylglycerols, triacylglycerols and sterols occurs within 20 min and is achieved using an octadecyl-silica column. Detection is performed by positive APCI mass spectrometry which provided sensitivity to detect over 50 compounds in the sample. After extraction of data, stepwise discriminant function analysis is used to select the variables with the highest discriminative power. These variables are used to perform linear discriminant analysis and classify/predict the samples. One-hundred per cent classification and 99% prediction rate was achieved for olive oils obtained from Nocellara, Biancolilla and Cerausola cultivars. Reliability of prediction was tested by cross validation.
Nagy
Kornél
K
Institute of Structural Chemistry, Chemical Research Center, Hungarian Academy of Sciences, H-1025 Pusztaszeri út 59-67, Budapest, Hungary.
Bongiorno
David
D
Avellone
Giuseppe
G
Agozzino
Pasquale
P
Ceraulo
Leopoldo
L
Vékey
Károly
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004075:Diglycerides / Q000302:isolation & purification; D016002:Discriminant Analysis; D013058:Mass Spectrometry / Q000379:methods; D000069463:Olive Oil; D010840:Phytosterols / Q000302:isolation & purification; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000302:isolation & purification
D004075:Diglycerides; D000069463:Olive Oil; D010840:Phytosterols; D010938:Plant Oils; D014280:Triglycerides