A novel yeast genomic DNA library on a geneticin-resistance vector.
We have constructed a Saccharomyces cerevisiae genomic library utilizing a novel centromere-containing vector bearing the TRP1 and KANMX4 G418-resistance selectable markers. Random Sau3AI genomic DNA fragments with an average size of 6.4 kb were introduced into a unique BamHI site in the vector's polylinker. Six independent library pools were generated and the characteristics of these pools were determined. This library can be used to introduce low-copy genomic DNA fragments into any strain that is not G418-resistant, making it useful for low-copy overexpression analysis or complementation of mutant phenotypes in strains that lack a suitable auxotrophy marker or only exhibit phenotypes on rich, undefined, growth media.
Jauert
Peter A
PA
Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall, Minneapolis, MN 55455, USA.
Jensen
Linnea E
LE
Kirkpatrick
David T
DT
D019747:Aldose-Ketose Isomerases / Q000235:genetics*; D000935:Antifungal Agents / Q000494:pharmacology; D004271:DNA, Fungal / Q000032:analysis; D025141:Drug Resistance, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D015698:Genomic Library; D005839:Gentamicins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014170:Transformation, Genetic
D000935:Antifungal Agents; D004271:DNA, Fungal; D005839:Gentamicins; D029701:Saccharomyces cerevisiae Proteins; C010680:antibiotic G 418; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae