Spontaneous growth of a laminin-apatite nano-composite in a metastable calcium phosphate solution.
We have previously reported that a laminin-apatite composite layer is formed on an ethylene-vinyl alcohol copolymer (EVOH) in a laminin-containing calcium phosphate (LCP) solution. In this work, the stability of the LCP solution and growth process of the laminin-apatite composite layer have been investigated. Dynamic light scattering technique revealed that the LCP solution was stable for periods as long as 24 h; it did not induce homogeneous precipitation of laminin or calcium phosphates in the solution. Analysis of the EVOH surface and the LCP solution showed that the laminin-apatite composite layer was formed via coprecipitation of laminin and apatite on the EVOH plate, i.e., spontaneous growing of apatite and simultaneous immobilization of laminin molecules or laminin-calcium phosphate nano-complexes onto its surface. Transmission electron microscopy also revealed that the laminin molecules in the resulting composite layer were not localized or aggregated, but were dispersed on a nano-scale in the entire layer. Because of this nano-composite structure, a large number of laminin molecules were stably immobilized on the EVOH plate. This may be responsible for the excellent cell adhesion properties of this type of composite material.
Oyane
Ayako
A
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. a-oyane@aist.go.jp
Uchida
Masaki
M
Onuma
Kazuo
K
Ito
Atsuo
A
D000818:Animals; D001031:Apatites / Q000737:chemistry*; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D008422:Materials Testing; D008854:Microscopy, Electron; D011145:Polyvinyls / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction
D001031:Apatites; D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D007797:Laminin; D011145:Polyvinyls; C020320:ethylene-vinyl alcohol copolymer; C020243:calcium phosphate