Surface-initiated growth of poly d(A-T) by Taq DNA polymerase.
In this paper, we report surface-initiated d(A-T) polymerization by Taq DNA polymerase as a method for constructing DNA-tethered surfaces using an enzyme. The enzymatic polymerization was conducted successfully via two steps: tethering of oligo d(A-T)s onto the surface presenting carboxylic acids by amide coupling and surface-initiated polymerization using Taq DNA polymerase. In this enzymatic polymerization process, the design and construction of carboxylic acid-presenting surfaces were found to be an important factor: DNA growth did not occur on the gold surface coated only with the self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA), but effectively proceeded on the surfaces presenting mixed SAMs of MHDA and 1-pentadecanethiol. The coupling of oligo d(A-T)s and the subsequent DNA polymerization reaction were characterized by polarized infrared external reflectance spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy.
Chi
Young Shik
YS
Department of Chemistry and School of Molecular Science (BK21), Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 Korea.
Jung
Young Hwan
YH
Choi
Insung S
IS
Kim
Yang-Gyun
YG
D006046:Gold; D018625:Microscopy, Atomic Force; D010169:Palmitic Acids; D011067:Poly dA-dT / Q000096:biosynthesis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013057:Spectrum Analysis; D013499:Surface Properties; D019914:Taq Polymerase / Q000737:chemistry / Q000378:metabolism; D014965:X-Rays
C450319:16-mercaptohexadecanoic acid; D010169:Palmitic Acids; D011067:Poly dA-dT; D006046:Gold; D019914:Taq Polymerase