Expression of noncoding mRNA in human blood cells activated with synthetic peptide of HIV.
T cells activation includes several steps such as translational events, activation of transcription for different genes, expression of surface molecules, secretion of cytokines, effectors functions. Knowledge has been accumulated on various nontranslatable RNA transcripts that are synthesized. In this context, a member of T cell noncoding transcripts (NTT) has been identified. It has been known that this gene is selectively expressed in activated T cells, as a 17-kb transcript. In this study, we investigate cell activation using RT-PCR to detect NTT. We investigated the expression of IFNgamma mRNA, a cytokine produced by activated blood mononuclear cells treated with HLA-A2 restricted synthetic peptide of HIV (p9) by RT-PCR detecting a fragment of 300 bp. This finding demonstrated that human HLA-A2 blood mononuclear cells have been activated in the presence of synthetic peptide of HIV (p9) and can induce expression of mRNA of NTT and IFNgamma which was confirmed by direct sequencing. For the first time, we have demonstrated an endogenous noncoding human RNA molecule, NTT mRNA, suggesting its implication in the cellular immune response.
Amarante
Marla Karine
MK
Department of Pathological Sciences, Londrina State University, Londrina, Paraná, Brazil.
De Lucca
Fernando Luiz
FL
de Oliveira
Carlos Eduardo Coral
CE
Pelegrinelli Fungaro
Maria Helena
MH
Reiche
Edna Maria Vissoci
EM
Muxel
Sandra Márcia
SM
Ehara Watanabe
Maria Angelica
MA
D001773:Blood Cells / Q000378:metabolism; D006678:HIV / Q000737:chemistry / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000187:drug effects; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D022661:RNA, Untranslated / Q000235:genetics*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000494:pharmacology*
D015789:HLA-A2 Antigen; D010446:Peptide Fragments; D022661:RNA, Untranslated; D014764:Viral Proteins