Gene structure and expression profile of Manduca sexta prophenoloxidase-activating proteinase-3 (PAP-3), an immune protein containing two clip domains.
Prophenoloxidase-activating proteinase-3 (PAP-3) is a component of the defence system in Manduca sexta. We have isolated genomic clones and elucidated the organization of this gene. The 3' end of exon 2, the entire exon 3 and the 5' end of exon 4 encode the two amino-terminal clip domains. Southern blot analysis suggested a single copy of the PAP-3 gene in the genome. We identified several putative immune-responsive elements in the upstream region. The PAP-3 gene is not highly expressed in the fat body during larval development until the wandering stage begins. The mRNA level is high in the epithelium, fat body and haemocytes. Tissue-specific alternative splicing occurs in the fat body and trachea. A bacterial injection markedly induced the gene expression in the fat body and haemocytes.
Zou
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Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, 74078, USA.
Jiang
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H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D018402:Manduca / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D012313:RNA; D012697:Serine Endopeptidases; C022435:prephenoloxidase-activating enzyme