Rapid detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery.
A gfp- and kanamycin-resistance gene-containing plasmid pUCGK was successfully constructed and transformed into Enterobacter aerogenes to develop a rapid GFP-based method for quantifying the bacterial concentration under anaerobic conditions for production of biohydrogen. Since the use of GFP as a molecular reporter is restricted by its requirement for oxygen in the development of the fluorophore, fluorescence detection for the fluorescent E. aerogenes grown anaerobically for hydrogen production was performed by developing a method of aerobic fluorescence recovery (AFR) of the anaerobically expressed GFP. By using this AFR method, rapid and non-disruptive cell quantification of E. aerogenes by fluorescence density was achieved for analyzing the hydrogen production process.
Zhang
Chong
C
Department of Chemical Engineering, Tsinghua University, Tsinghua Yuan, Beijing 100084, PR China. zhangchong98@mails.tsinghua.edu.cn
Xing
Xin-Hui
XH
Lou
Kai
K
D000332:Aerobiosis; D000693:Anaerobiosis; D002455:Cell Division; D017931:DNA Primers; D021902:Enterobacter aerogenes / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D007700:Kinetics; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence
D017931:DNA Primers; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins