Introducing the wide range C-reactive protein (wr-CRP) into clinical use for the detection of microinflammation.
BACKGROUND
The determination of low grade inflammation in apparently healthy individuals (microinflammation) has prognostic significance in terms of future vascular events and accelerated atherothrombotic disease.


METHODS
We compared the Bayer wide range (wr)-C-reactive protein (CRP) immunoturbidometric assay on the ADVIA 1650 system to the Dade Behring high sensitivity (hs)-CRP on the BNII Nephelometer in 1446 apparently healthy individuals having a relatively low (<10 mg/l) concentration. The correlation between the 2 assays was also analyzed in relation to other commonly used microinflammatory biomarkers.


RESULTS
A significant (p<0.0005) correlation was noted between the hs-CRP and the wr-CRP for the entire cohort (r=0.99) as well as for both women (r=0.99 n=483) and men (r=0.99 n=963). The mean difference between the measures (hs-CRP minus wr-CRP) was -0.039 (SD 0.317). The Deming regression results for the entire cohort showed a slope of 1.112+/-0.004 and an intercept of -0.263+/-0.01.


CONCLUSIONS
The Bayer wr-CRP assay performed presents a reasonable alternative to the Behring Dade hs-CRP assay. The advantages of the wr-CRP assay are its online and real time availability as well as lower costs.
Rogowski
Ori
O
Department of Medicine D and Institute for Special Medical Examinations (MALRAM), Tel Aviv Sourasky Medical Center, Tel Aviv, affiliated to Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 6 Weizman Street, Tel Aviv 64239, Israel.
Vered
Yaffa
Y
Shapira
Itzhak
I
Hirsh
Meirav
M
Zakut
Vera
V
Berliner
Shlomo
S
D000328:Adult; D002097:C-Reactive Protein / Q000032:analysis* / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012307:Risk Factors; D016896:Treatment Outcome
D002097:C-Reactive Protein