[Identification of nm23-M2/NDPK B expression during the blastocyst adhesiveness in the mouse endomerium by two-dimentional electrophoresis and MALDI-TOF-MASS spectrometry].
The nm23 gene family was involved in cellular multiphysiopathological processes including differentiation, development, apoptosis and cancer promotion, progression or metastasis. Some data indicate that nm23 plays an important role in regulating reproductive processes. In the present study, we analyzed the proteome of the implantation sites and the peri-implantation sites in NIH. mice on Day 5 of gestation by using two-Dimensional gel electrophoresis (2-D PAGE), while the virgin mice as the control. A protein spot with pI 7.1, Mr 18 kDa showed up-regulated expression in endometrium during the blastocysts adhesiveness. Using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), this protein was identified as nm23-M2/NDPK B. The nm23-M2 expression in mice endometrium was shown progressive increase on Day 5 of gestation by RT-PCR, which was consistent with the result obtained by immunohistochemistry. These findings suggest that nm23-M2/NDPK B was involved in the process of blastocyst implantation.
Pan
Wei
W
Department of Reproductive Medical Science, Chongqing University of Medical Sciences,Chongqing 400016; China. pw_6602@sina.com
Wang
Ying Xiong
YX
Li
Gang
G
Weng
Ya Guang
YG
Liu
Fang
F
Yu
Qui Bo
QB
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D054778:NM23 Nucleoside Diphosphate Kinases