Expression analysis of genes responsible for serotonin signaling in the brain.
To thoroughly understand the function and regulation of neurotransmitter systems in the brain, as well as the underlying disease mechanisms, it is important to comprehensively analyze the expression patterns of genes participating in such systems. Using functional annotated cDNA clones (FANTOM), we examined the gene expression patterns of the serotonin neurotransmitter system, which is involved in psychiatric diseases such as depression. We chose 24 gene products and visualized their endogenous localizations using in situ hybridization (ISH). We were able to fine-tune an automated ISH method to obtain high-resolution cell-based figures within 24 h. We also measured the amounts of mRNAs with quantitative RT-PCR. The outline of the in situ gene expression pattern viewed under low magnification agreed with the results of the RT-PCR. In the high-resolution view obtained with ISH, we could document novel localizations of the several genes critically related to serotonin activity.
Ichikawa
Manabu
M
Laboratory for Genome Exploration Research Group,RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Okamura-Oho
Yuko
Y
Okunishi
Rie
R
Kanamori
Mutsumi
M
Suzuki
Harukazu
H
Ritani
Akishige
A
Nitta
Hiroaki
H
Eguchi
Naomi
N
Urade
Yoshihiro
Y
Hayashizaki
Yoshihide
Y
D000818:Animals; D001921:Brain / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*
D012333:RNA, Messenger; D012701:Serotonin