[Study on the reaction mechanism of chloroacetanilide herbicides with urease using fluorescence spectrum and high-performance liquid chromatography].
The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
Liu
Hui-jun
HJ
Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China.
Zhan
Xiu-Ming
XM
Li
Ke-bin
KB
Liu
Wei-ping
WP
D000081:Acetamides / Q000737:chemistry* / Q000378:metabolism; D000083:Acetanilides / Q000737:chemistry; D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D015394:Molecular Structure; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014052:Toluidines / Q000737:chemistry; D014510:Urease / Q000737:chemistry* / Q000378:metabolism
D000081:Acetamides; D000083:Acetanilides; D014052:Toluidines; C118050:pretilachlor; C078188:2-chloro-N-(ethoxymethyl)-N-(2-methyl-6-(trifluoromethyl)phenyl)acetamide; C043377:acetochlor; C054409:butachlor; D014510:Urease; C051786:metolachlor