[Synthesis of a bio-active bone-matrix material and study of the cellular biocompatibility].
To prepare poly(lactic acid/glycolic acid/ asparagic acid-co- polyethylene glycol) (PLGA-[ASP-PEG]) and examine the cellular biocompatibility. PLGA-[ASP-PEG] was obtained by bulk ring-opening copolymerization method, examined by infrared spectrometry (IR) and 1H nuclear magnetic resonance spectroscopy (1H NMR). Bone marrow stromal cells(BMSCs) were cultured with PLGA-[ASP-PEG] (experiment gruop) and PLGA (control group) in vitro respectively, and were observed by phase-contrast microscopy and scanning electron microscopy. The resuls showed that PLGA-[ASP-PEG] was obtained and proved by IR and 1H NMR. The BMSCs of the experiment group could well attach to and extend on the surface of the PLGA-[ASP-PEG], and could proliferate and secrete better extracellular matrix, compared with control. The PLGA-[ASP-PEG] has good cellular a biocompatibility. It can be used as a biomaterial for bone tissue engineering.
Hao
Jie
J
Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong Science and Technology University, Wuhan 430022, China. hjie2005@yahoo.com.cn
Zheng
Qixin
Q
Guo
Xiaodong
X
Quan
Daping
D
Luo
Binghong
B
D001224:Aspartic Acid / Q000737:chemistry; D001672:Biocompatible Materials; D001857:Bone Matrix / Q000166:cytology*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D001224:Aspartic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide)