Role of intramolecular interaction in connexin50: mediating the Ca2+-dependent binding of calmodulin to gap junction.
Gap junction channels formed by connexin50 (Cx50) are critical for maintenance of eye lens transparency. Cleavage of the carboxyl terminus (CT) of Cx50 to produce truncated Cx50 (Cx50trunc) occurred naturally during maturation of lens fiber cells. The mechanism of its altered properties is under confirmation. It has been suggested that calmodulin (CaM) participates in gating some kinds of gap junction. Here, we performed confocal colocalization and co-immunoprecipitation experiments to study the relationships between Cx50 and CaM. Results exhibited that the CaM could colocalize Ca2+ dependently with CT in the linear area of cell-to-cell contact formed by Cx50trunc, while it could not localize in the linear area without expression of CT. Further study indicated that the CT could interact Ca2+ independently with the cytoplasmic loop (CL) of Cx50. These data put forward the importance of Ca2+-independent intramolecular interaction between CT and CL of Cx50, which mediate the Ca2+-dependent binding of CaM to Cx50. These intra- and intermolecular interactions may further improve our understanding of biological significance of the Cx50 in the eye lens.
Zhang
Xianrong
X
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei Province, China.
Qi
Yipeng
Y
D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism*; D017630:Connexins / Q000737:chemistry / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D017629:Gap Junctions / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D007473:Ion Channels / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism / Q000648:ultrastructure; D000099264:Connexin 50
D002118:Calcium; D002147:Calmodulin; D017630:Connexins; D005136:Eye Proteins; D007473:Ion Channels; D000099264:Connexin 50