title: Molecular identification of pathogenic house dust mites using 12S rRNA sequences.
abstract: House dust mites are microarthropods implicated in the cause of allergic diseases. Currently, there is no phylogenetic analysis of dust mites based on genomic or mitochondrial DNA (mtDNA) evidence. For the first time, we report evolutionary relationships based on partial mtDNA 12S rRNA sequences among the four dust mite families Pyroglyphidae (Dermatophagoides pteronyssinus), Glycyphagoidea (Glycyphagus privatus), Acaridae (Aleuroglyphus ovatus), and Echimyopodidae (Blomia tropicalis). Thirteen sequence variants were obtained and phylogenetic analysis showed two monophyletic clades composed of two species each. Contrary to current taxonomic classification, the Acaridae clustered in a monophyletic group with the Pyroglyphidae. Considering the current difficulties in identifying these medically important species for the purpose of eradication and treatment, it is significant that sequence data are capable of discriminating between species belonging to different families of dust mites.
authors: Molecular Biology and Human Diversity Laboratory, Department of Biological Sciences/OE 304, Florida International University, Miami, 33199, USA.
authors: Edu B
authors: EB
authors: Suarez-Martinez
authors: Federico
authors: F
authors: Montealegre
authors: Julie M
authors: JM
authors: Sierra-Montes
authors: Rene J
authors: RJ
authors: Herrera
mesh_terms: D040041:Acaridae / Q000145:classification / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D040002:Dermatophagoides pteronyssinus / Q000145:classification / Q000235:genetics; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D039981:Pyroglyphidae / Q000145:classification / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S