title: Assessment of IgM enzyme immunoassay and IgG avidity assay for distinguishing between primary and secondary immune response to rubella vaccine.
abstract: The primary test for the laboratory confirmation of rubella is IgM serology. It is important to distinguish IgM reactivity caused by primary infection from that caused by reinfection or persistence, especially in pregnant women; as termination of pregnancy is considered when primary rubella is diagnosed during the first trimester. In this study, the performance of rubella IgM enzyme immunoassay (IgM-EIA) and rubella IgG avidity assay were compared using well-defined panels of sera from persons vaccinated against rubella and commercial rubella IgM and IgG enzyme immunoassay kits (Dade Behring, Marburg, Germany). The sensitivity and specificity of rubella IgM-EIA were found to be 77.4 and 97.9%, respectively, while the results for rubella IgG avidity assay were 100 and 100%. IgG avidity assay showed higher positive and negative predictive values than the IgM-EIA (100 and 100% compare to 96.9 and 82.9%). In conclusion, the rubella IgG avidity assay is more sensitive and specific than IgM-EIA for differential detection of primary rubella infection from rubella reinfection.
authors: School of Public Health, Tehran University of Medical Sciences, Tehran 14155, Iran. rhamkar@sina.tums.ac.ir
authors: Rasool
authors: R
authors: Hamkar
authors: Somayeh
authors: S
authors: Jalilvand
authors: Talat
authors: T
authors: Mokhtari-Azad
authors: Keramat
authors: K
authors: Nouri Jelyani
authors: Hosein
authors: H
authors: Dahi-Far
authors: Hoorieh
authors: H
authors: Soleimanjahi
authors: Rakhshandeh
authors: R
authors: Nategh
mesh_terms: D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012409:Rubella / Q000097:blood / Q000276:immunology*; D012411:Rubella Vaccine / Q000008:administration & dosage*; D012412:Rubella virus / Q000276:immunology*; D013997:Time Factors; D014611:Vaccination
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D012411:Rubella Vaccine