title: Effects of raw biles and their non-protein fractions from fox, pig, sheep and chicken on the survival of Trichinella spp. in vitro.
abstract: The in vitro differential effect of fox, pig, sheep and chicken bile and corresponding non-protein fractions at various concentrations on the motility of released muscle larvae of Trichinella spiralis, Trichinella nativa and Trichinella nelsoni was examined. In many cases, the percentages of motile (live) larvae of the three Trichinella species cultured in the presence of the non-protein fractions of bile from the study animals were significantly higher (p<0.001) compared to their respective control cultures. In addition, the percentages of motile (live) larvae of all Trichinella species cultured in the presence of the non-protein fraction of bile at every concentration from all study animals were significantly higher (p<0.001) compared to their respective cultures in the presence of raw bile. Not only did results with non-protein fractions of bile differ from those with raw bile, but also the non-protein fraction, with increased dilution, showed a decrease in the percentages of motile larvae while the opposite was true with the raw bile (p<0.001). These observations indicate that the non-protein fraction of bile prolongs the in vitro survival of larvae.
authors: Department of Anatomy and Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens, 75 Iera Odos, Votanikos, Athens 11855, Greece. gtheo@aua.gr
authors: G
authors: G
authors: Theodoropoulos
authors: M
authors: M
authors: Prokou
authors: V
authors: V
authors: Georgiadou
authors: M
authors: M
authors: Petrakos
authors: P
authors: P
authors: Webster
authors: C M O
authors: CM
authors: Kapel
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D005589:Foxes / Q000378:metabolism*; D051379:Mice; D012756:Sheep / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D014234:Trichinella / Q000254:growth & development* / Q000378:metabolism