[Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC].
OBJECTIVE
To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.


METHODS
The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm.


RESULTS
The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.


CONCLUSIONS
The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.
Lin
Sheng
S
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhang
Qi-Wei
QW
Zhang
Ning-Ning
NN
Zhang
Yong-Xin
YX
D019306:Artemisia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D047309:Flavones / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D047309:Flavones; C039824:cirsiliol; C057618:cirsilineol; C007072:cirsimaritin