title: Characterization of the ferrichrome A biosynthetic gene cluster in the homobasidiomycete Omphalotus olearius.
abstract: Under iron deprivation Omphalotus olearius was found to produce the hydroxamate siderophore ferrichrome A. A gene cluster consisting of three genes: fso1, a nonribosomal peptide synthetase whose expression is enhanced in the absence of iron; omo1, a l-ornithine-N(5)-monooxygenase; and ato1, an acyltransferase probably involved in the transfer of the methylglutaconyl residue to N(5)-hydroxyorinithine was identified. The fso1 sequence is interrupted by 48 introns and its derived protein sequence has a similar structure to the homologous genes of Ustilago maydis and Aspergillus nidulans. This is the first report of a nonribosomal peptide synthetase gene and a biosynthetic gene cluster in homobasidiomycetes.
authors: Institut für Biotechnologie und Wirkstoff-Forschung e.V. (IBWF), Erwin-Schrödinger-Str. 56, D-67663 Kaiserslautern, Germany.
authors: Kai
authors: K
authors: Welzel
authors: Katrin
authors: K
authors: Eisfeld
authors: Luis
authors: L
authors: Antelo
authors: Timm
authors: T
authors: Anke
authors: Heidrun
authors: H
authors: Anke
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000363:Agaricales / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000595:Amino Acid Sequence; D005291:Ferrichrome / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D007501:Iron / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D005291:Ferrichrome; D007501:Iron; D006899:Mixed Function Oxygenases; C059419:ornithine N5-oxygenase; D000217:Acyltransferases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase