Effect of JP-8 jet fuel exposure on protein expression in human keratinocyte cells in culture.
Dermal exposure to jet fuel is a significant occupational hazard. Previous studies have investigated its absorption and disposition in skin, and the systemic biochemical and immunotoxicological sequelae to exposure. Despite studies of JP-8 jet fuel components in murine, porcine or human keratinocyte cell cultures, proteomic analysis of JP-8 exposure has not been investigated. This study was conducted to examine the effect of JP-8 administration on the human epidermal keratinocyte (HEK) proteome. Using a two-dimensional electrophoretic approach combined with mass spectrometric-based protein identification, we analyzed protein expression in HEK exposed to 0.1% JP-8 in culture medium for 24 h. JP-8 exposure resulted in significant expression differences (p<0.02) in 35 of the 929 proteins matched and analyzed. Approximately, a third of these alterations were increased in protein expression, two-thirds declined with JP-8 exposure. Peptide mass fingerprint identification of effected proteins revealed a variety of functional implications. In general, altered proteins involved endocytotic/exocytotic mechanisms and their cytoskeletal components, cell stress, and those involved in vesicular function.
Witzmann
F A
FA
Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, 1345 W 16th Street, Rm 308, Indianapolis, IN 46202-2111, USA. fwitzman@iupui.edu
Monteiro-Riviere
N A
NA
Inman
A O
AO
Kimpel
M A
MA
Pedrick
N M
NM
Ringham
H N
HN
Riviere
J E
JE
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006838:Hydrocarbons / Q000494:pharmacology* / Q000633:toxicity; D007091:Image Processing, Computer-Assisted; D016209:Interleukin-8 / Q000096:biosynthesis; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism*; D016273:Occupational Exposure; D010449:Peptide Mapping; D014176:Protein Biosynthesis / Q000187:drug effects*; D020543:Proteome / Q000096:biosynthesis / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006838:Hydrocarbons; D016209:Interleukin-8; C098607:JP8 aviation fuel; D020543:Proteome