title: [Discovery of the main active constituents of siwu mixture for reversing the multidrug resistance of human erythrocyte leukemic cell line K562/ADM].
abstract: OBJECTIVE
  To study the main active constituents of Siwu mixture for reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM.
  
  
  METHODS
  Macroporous resin was used to separate Siwu mixture and an in vitro model was used to evaluate the reversal index of each fraction for discovery of active fraction. HPLC-MS was used for investigating the main active constituents of Siwu mixture.
  
  
  RESULTS
  The reversal index of the gross polysaccharide of Rehmannia glutinosa was significantly higher than that of blank control (P < 0.01). There was little difference between the reversal effect of the gross polysaccharide of Rehmannia glutinosa and that of the active fraction A (P > 0.05).
  
  
  CONCLUSIONS
  The gross polysaccharide of Rehmannia glutinosa is one of the main active constituents of Siwu mixture.
authors: The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. shenp@zju.edu.cn
authors: Peng
authors: P
authors: Shen
authors: Lei
authors: L
authors: Xia
authors: Hai-jiang
authors: HJ
authors: Zhang
mesh_terms: D004317:Doxorubicin; D004338:Drug Combinations; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D030004:Ligusticum / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C403258:si-wu-tang; D004317:Doxorubicin