[Study on quality control of effective fraction in qixue bingzhi decoction].
OBJECTIVE
To develop a method for quality control of effective fraction in Qi-Xue-Bing-Zhi decoction, a traditional Chinese medicine (TCM).


METHODS
PF samples, effective fraction from Qi-Xue-Bing-Zhi decoction, were used as example, and a HPLC assay for chemical fingerprint and quantitative analysis was established.


RESULTS
The contents range of Paeoniflorin (PE), Naringin (NG) and Neohesperidin (NH) in effective fractions were changed from 12.5%-16.0%, 8.4%-12.4%, 12.8%-15.3%, and their average contents were (14.7 +/- 1.1)%, (10.6 +/- 1.2)%, (14.2 +/- 0.8)% (n = 10), respectively. The fingerprints of PF samples showed 25 common peaks, and the fingerprint similarity for PF samples were all above 99.00% by comparing with the standard chromatogram.


CONCLUSIONS
The method reported could be used effectively for the quality control of effective fraction from TCM.
Huang
Zai-Fang
ZF
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
Luan
Lian-Jun
LJ
Shao
Qing
Q
Cheng
Yi-Yu
YY
D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D044950:Flavanones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D006569:Hesperidin / Q000031:analogs & derivatives / Q000032:analysis; D030004:Ligusticum / Q000737:chemistry; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D006569:Hesperidin; D039821:Monoterpenes; C101234:neohesperidin monoglucoside; C015423:peoniflorin; D006027:Glycosides; C005274:naringin