title: Effect of pulsed magnetic field on regenerating rat sciatic nerve: an in-vitro electrophysiologic study.
abstract: Some experimental studies report that low-frequency pulsed electromagnetic field (PEMF) stimulation may accelerate regeneration in peripheral nerves. In the present study, effects of PEMF on the regeneration of the crushed rat sciatic nerves were investigated with histological and in-vitro electrophysiological methods (sucrose-gap). After crush injury of the sciatic nerves, rats were divided into 5, 15, 25, 38 day-groups and exposed to PEMF (1.5 h/day, intensity; 1.5 mT, consecutive frequency; 10-40-100 Hz). In the 15th day post crush, compound action potential (CAP) amplitude was measured as 5.5+/-1 mV (crush group) and 5.4+/-1.2 mV (crush+PEMF group). In addition, half width of CAP extended ~3 fold in both groups and frequency-dependent amplitude inhibition (FDI) decreased approximately 20% at 100 Hz. In the 38th day, amplitude of CAP, half width of CAP and FDI were measured nearly intact nerve values in both groups. In histological examinations, Wallerian degeneration was observed similar progress between both groups. The results were compared between crush and crush + PEMF groups, it was found that the effect of PEMF was not significant. The authors conclude that PEMF were ineffective on rat sciatic nerve regeneration.
authors: Cukurova University, Medical School, Department of Biophysics, Adana, Turkey. guvenm@cu.edu.tr
authors: Mustafa
authors: M
authors: Güven
authors: Ismail
authors: I
authors: Günay
authors: Kerem
authors: K
authors: Ozgünen
authors: Suzan
authors: S
authors: Zorludemir
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D004574:Electromagnetic Fields; D005260:Female; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology*