Technical aspects of immunohistochemistry.
Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immunohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.
Ramos-Vara
J A
JA
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA. ramosja@purdue.edu
D000438:Alcohols / Q000737:chemistry; D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification*; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods* / Q000662:veterinary*; D046228:Microarray Analysis / Q000379:methods / Q000662:veterinary; D011506:Proteins / Q000737:chemistry
D000438:Alcohols; D000906:Antibodies; D000941:Antigens; D005404:Fixatives; D007136:Immunoglobulins; D011506:Proteins; D005557:Formaldehyde