Two-dimensional electrophoresis database of fluorescence-labeled proteins of colon cancer cells.
We constructed a novel database of the proteome of DLD-1 colon cancer cells by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of fluorescence-labeled proteins followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis. The database consists of 258 functionally categorized proteins corresponding to 314 protein spots. The majority of the proteins are oxidoreductases, cytoskeletal proteins and nucleic acid binding proteins. Phosphatase treatment showed that 28% of the protein spots on the gel are phosphorylated, and mass spectrometric analysis identified 21 of them. Proteins of DLD-1 cells and of laser-microdissected colon cancer tissues showed similar distribution on 2D gels, suggesting the utility of our database for clinical proteomics.
Mori
Yasuharu
Y
Cancer Proteomics Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Tokyo 104-0045, Japan.
Kondo
Tadashi
T
Yamada
Tesshi
T
Tsuchida
Akihiko
A
Aoki
Tetsuya
T
Hirohashi
Setsuo
S
D000595:Amino Acid Sequence; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002232:Carbocyanines; D010750:Phosphoproteins; D011506:Proteins; C089187:cyanine dye 3; C085321:cyanine dye 5; D010744:Phosphoric Monoester Hydrolases