Determination of ANA specificity using the UltraPlex platform.
SmartBead Technologies has developed a multiplexed immunofluorescence assay, the UltraPlex ANA Profile, which determines nine antinuclear antibodies simultaneously. The UltraPlex assay platform uses bar-coded microparticles to track analytes through assays. These bar-coded microparticles were used to tag and track key ANA markers: Jo-1, Scl-70, Sm, SmRNP, SSA, SSB, U1RNP, Centromere B, dsDNA, and a blank control microparticle. The immunofluorescence assays are fully automated and are performed on a Perkin-Elmer multiprobe II liquid handling system that performs all sera dilutions, additions of reagents, washes, and incubation steps. Results were determined by the automated UltraPlex plate reader. This fully automated multiplex antinuclear antibody (ANA) immunoassay was used to screen commercially available ANA-positive sera and negative control samples. The UltraPlex ANA Profile enables the panels of samples to be screened simultaneously for nine ANA antoantibodies, requiring significantly less labor and fewer reagents, with performance equivalent to existing gold-standard methods.
Smith
Jodie
J
SmartBead Technologies Limited, Babraham Hall, Babraham, Cambridge CB2 4AT, United Kingdom. jsmith@smartbead.com
Onley
David
D
Garey
Caroline
C
Crowther
Stuart
S
Cahir
Nicholas
N
Johanson
Adam
A
Painter
Sianie
S
Harradence
Grant
G
Davis
Ricardo
R
Swarbrick
Peter
P
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis / Q000097:blood; D015415:Biomarkers / Q000032:analysis; D001330:Electronic Data Processing; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D015200:Fluoroimmunoassay; D006801:Humans; D008854:Microscopy, Electron; D008863:Microspheres; D012680:Sensitivity and Specificity
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D015415:Biomarkers