World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays.
We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 degrees C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.
Oh
Kwang W
KW
Bio Lab, Samsung Advanced Institute of Technology, P.O. Box 111, Suwon 440-600, Korea. MrOhio@Samsung.com
Park
Chinsung
C
Namkoong
Kak
K
Kim
Jintae
J
Ock
Kyeong-Sik
KS
Kim
Suhyeon
S
Kim
Young-A
YA
Cho
Yoon-Kyoung
YK
Ko
Christopher
C
D004279:DNA, Viral / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral