[Study on relationships between seven microsatellite loci and somatic cell score in Beijing Holstein cows].
Genetic variation of seven microsatellite loci BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 which were closely linked to somatic cell score (SCS) was analyzed in 240 Beijing Holstein cows with nondenaturing polyacrylamide gel electrophoresis. Allele frequencies, heterozygosity, polymorphic information content, the effective number of alleles of seven microsatellite loci were calculated. Relationships between seven microsatellite loci and somatic cell score in Beijing Holstein cows were primarily analyzed by least squares linear model. Least squares means of SCS for BM1818 (284 bp/284 bp), BM1258 (106 bp/92 bp), BM1443 (166 bp/160 bp), BM1905 (187 bp/187 bp), BM302 (142 bp/140 bp), BM4505 (240 bp/236 bp) and CYP21 (215 bp/198 bp) were relatively lower,and their genotypes were the most favorable genotypes in respective locus for mastitis resistance. Least squares means of SCS for BM1818 (286 bp/286 bp), BM1258 (102 bp/102 bp), BM1443 (170 bp/160 bp), BM1905 (197 bp/195 bp), BM302 (154 bp/145 bp), BM4505 (240 bp/238 bp) and CYP21 (204 bp/192 bp) were relatively higher,and their genotypes were the most unfavorable genotypes in respective locus for mastitis resistance. The information found in the present study would be very important for improving mastitis resistance in dairy cattle by marker assisted selection.
Chu
Ming-Xing
MX
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China. mxchu@263.net
Zhou
Guo-Li
GL
Jin
Hai-Guo
HG
Shi
Wan-Hai
WH
Cao
Fu-Cun
FC
Fang
Li
L
Ye
Su-Cheng
SC
Zhu
Yan
Y
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002452:Cell Count; D002681:China; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D005838:Genotype; D008414:Mastitis, Bovine / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008892:Milk / Q000166:cytology*; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable