title: [Inhibition of hepatitis B virus by combined siRNAs in vitro].
abstract: Small interfering RNA (siRNA) technology is a powerful tool to knockdown gene expression in mammalian cells including genes of viral origin. To study the inhibition of hepatitis B virus (HBV) by combined siRNAs in vitro, HepG2 2.2.15, a human hepatoblastoma cell that constitutively produces HBV particles, was transfected with 12 siRNAs that were designed to target different regions on HBV genome. Enzyme-linked immunosorbent assay was used to measure the HBsAg and HBeAg levels in culture media, and HBV replication was determined by real-time quantitative PCR. The results showed that HBV replication could be inhibited by treatment with these siRNAs in a dose-dependent manner. The expression of HBsAg was inhibited by 80% and 90% with 80 nmol/L single siRNA treatment and 20 nmol/L combined siRNAs treatment, respectively. HBeAg was inhibited by about 60% with single siRNA treatment, and the inhibition didn't increase much in the case of combined siRNAs treatment. HBV replication was inhibited by 90% with 80 nmol/L single siRNA treatment or 20 nmol/L combined siRNAs treatment. These data provide solid evidence for developing new approaches in HBV treatment with combined siRNAs.
authors: Cancer Institute, the Second Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310009, China.
authors: Jing Jia
authors: JJ
authors: Ye
authors: Ze Feng
authors: ZF
authors: Xu
authors: Zhe
authors: Z
authors: Chen
authors: Jiang
authors: J
authors: Cao
authors: Shu
authors: S
authors: Zheng
authors: Jia Yi
authors: JY
authors: Ding
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D006514:Hepatitis B Surface Antigens; D034741:RNA, Small Interfering