Genome-wide RNAi screen for host factors required for intracellular bacterial infection.
Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.
Agaisse
Hervé
H
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. herve.agaisse@yale.edu
Burrack
Laura S
LS
Philips
Jennifer A
JA
Rubin
Eric J
EJ
Perrimon
Norbert
N
Higgins
Darren E
DE
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D003600:Cytosol / Q000382:microbiology; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D017344:Genes, Insect; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008089:Listeria monocytogenes / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology; D019907:Mycobacterium fortuitum / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology; D010641:Phenotype; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000494:pharmacology; D015398:Signal Transduction / Q000235:genetics; D014617:Vacuoles / Q000382:microbiology; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; D012330:RNA, Double-Stranded; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins