title: SNAREs can promote complete fusion and hemifusion as alternative outcomes.
abstract: Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run-like events.
authors: Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
authors: Claudio G
authors: CG
authors: Giraudo
authors: Chuan
authors: C
authors: Hu
authors: Daoqi
authors: D
authors: You
authors: Avram M
authors: AM
authors: Slovic
authors: Eugene V
authors: EV
authors: Mosharov
authors: David
authors: D
authors: Sulzer
authors: Thomas J
authors: TJ
authors: Melia
authors: James E
authors: JE
authors: Rothman
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D008561:Membrane Fusion / Q000235:genetics / Q000502:physiology*; D051379:Mice; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins