[Discovery of the main active constituents of siwu mixture for reversing the multidrug resistance of human erythrocyte leukemic cell line K562/ADM].
OBJECTIVE
To study the main active constituents of Siwu mixture for reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM.


METHODS
Macroporous resin was used to separate Siwu mixture and an in vitro model was used to evaluate the reversal index of each fraction for discovery of active fraction. HPLC-MS was used for investigating the main active constituents of Siwu mixture.


RESULTS
The reversal index of the gross polysaccharide of Rehmannia glutinosa was significantly higher than that of blank control (P < 0.01). There was little difference between the reversal effect of the gross polysaccharide of Rehmannia glutinosa and that of the active fraction A (P > 0.05).


CONCLUSIONS
The gross polysaccharide of Rehmannia glutinosa is one of the main active constituents of Siwu mixture.
Shen
Peng
P
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. shenp@zju.edu.cn
Xia
Lei
L
Zhang
Hai-jiang
HJ
D004317:Doxorubicin; D004338:Drug Combinations; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D030004:Ligusticum / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C403258:si-wu-tang; D004317:Doxorubicin