title: [Expressions and role of endogenetic matrix metalloproteinases-9 and transforming growth factor-beta during wound healing of blast injury].
abstract: OBJECTIVE
  To investigate the expression of endogenetic matrix metalloproteinases-9 (MMP-9) and transforming growth factor-beta(TGF-beta) and their role in the wound healing of blast injury.
  
  
  METHODS
  Rat models of blast injury under a humid and hot environment were established and the effusion from the wound surface was collected at 4, 24, 48 h and 5, 7, 14, 21 and 28 days after injury, respectively. The contents of MMP-9 and TGF-beta in the effusion of the wound were measured by zymography and enzyme-linked immunosorbent assay (ELISA), respectively.
  
  
  RESULTS
  During the wound healing of blast injury, MMP-9 and TGF-beta exhibited changes that followed a regular pattern, both reaching the peak value at 48 h after the injury. TGF-beta content reached the another peak on day 7. TGF-beta value and MMP-9 contents decreased in the second week after injury and their reduction was no longer parallel. Administration of tissue inhibitor of metalloproteinase (TIMP) in the early phase of injury showed no obvious effect, but during the 2 weeks after the injury, its administration caused decrease in MMP-9 content and increase in TGF-beta content in the effusion.
  
  
  CONCLUSIONS
  In the early phase of wound healing, the elevation of MMP-9 and TGF-beta accelerated cell migration to promote the clearance of the inflammatory necrosis tissues, which might be one of the wound healing mechanisms. But overexpression of MMP-9 in the wound may hinder wound healing, and appropriate use of TIMP can accelerate the delayed wound healing.
authors: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zxy517710@sohu.com
authors: Xin-yong
authors: XY
authors: Zhu
authors: Chi-hua
authors: CH
authors: Fang
authors: Li-yun
authors: LY
authors: Zhang
authors: Da-ming
authors: DM
authors: Zuo
authors: Zheng-liang
authors: ZL
authors: Chen
mesh_terms: D000818:Animals; D001753:Blast Injuries / Q000378:metabolism*; D005260:Female; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D016212:Transforming Growth Factor beta; D020780:Matrix Metalloproteinase 9