title: In vitro culture of various typed meningiomas and characterization of a human malignant meningioma cell line (HKBMM).
abstract: We placed on culture the 13 cases of meningiomas, succeeded in making a primary culture of 10 cases and maintained 5 cases in vitro over considerable period of time (over three month), and one cell line derived from a malignant meningioma were established. In the early period of the primary culture, meningioma cells were spindle- or round-shaped cells. In the case of psammomatous type, the cultured cells were characterized as forming psammoma bodies. A cell line designated "HKBMM" was established from a human malignant meningioma occurred from frontal lobe. This line grew well without interruption for 5 years and was subcultivated over 120 times. The cells were spindle and fibrous in shape, and neoplastic and pleomorphic features, and multilayering without contact inhibition. The cells proliferated rapidly, and the population doubling time was about 29 hours. The chromosome number showed a wide distribution of aneuploidy. The mode was in the diploid range. The culture cells were easily transplanted into the subcutis of nude mice and produced the tumor resembling the original tumor.
authors: Ishiwata Obstetrics & Gynecology Hospital, Mito-shi, Ibaraki-ken, Japan.
authors: Isamu
authors: I
authors: Ishiwata
authors: Chieko
authors: C
authors: Ishiwata
authors: Emiko
authors: E
authors: Ishiwata
authors: Yoshiro
authors: Y
authors: Sato
authors: Kazushige
authors: K
authors: Kiguchi
authors: Toshiaki
authors: T
authors: Tachibana
authors: Hiroshi
authors: H
authors: Ishikawa
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D007621:Karyotyping; D008579:Meningioma / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation