[The relationship between the expression of HIF-1alpha and the angiogenesis in infancy hemangioma].
OBJECTIVE
To investigate the expression of HIF-1alpha and its roles on the vascular endothelial cell growth factor (VEGF) and microvessel density (MVD ) in hemangioma.


METHODS
Immunohistochemical streptavidin/peroxidase(SP) stain was applied to examine the expression of HIF-1alpha, VEGF and CD34. The amount of microvessel was counted, which the endothelial cell expressed CD34.


RESULTS
The nuclei and cytoplasm of the endothelial cell showed positive staining for HIF-1alpha in hemangioma. In 28 cases, positive rate for HIF-1alpha was 64%, for VEGF was 71.4%. In the proliferating phase, positive rate for HIF-1alpha was 87.5%, for VEGF was 93.7%. However, the positive rate for HIF-1alpha, in the involuting phase, was 33.3%, and for VEGF was 41.7%. There was a very significantly different between two phases (P < 0.01). In the proliferating phase, MVD was 73.4 +/- 14.63 . In the involuting phase, MVD was 30.2 +/- 9.1 (P < 0.01). The correlation between the HIF-1alpha and VEGF was positive (P < 0.01). There was a positive correlation between the HIF-1alpha and MVD (P < 0.01).


CONCLUSIONS
There might be a special hypoxia microenvironment in endothelial cell of the hemangioma in proliferating phase , which could up-regulate the expression of the HIF-1alpha protein. The HIF-1alpha protein could also up-regulate the VEGF protein and then result in the angiogenesis.
Chen
Da
D
Department of Plastic Surgery, Ninth Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Lin
Xiao-Xi
XX
Li
Wei
W
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006391:Hemangioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A