title: Differentiation of neuroblastoma cell line N1E-115 involves several signaling cascades.
abstract: No systematic searches for differential expression of signaling proteins (SP) in undifferentiated vs. differentiated cell lineages were published and herein we used protein profiling for this purpose. The NIE-115 cell line was cultivated and an aliquot was differentiated with dimethylsulfoxide (DMSO), that is known to lead to a neuronal phenotype. Cell lysates were prepared, run on two-dimensional gel electrophoresis followed by MALDI-TOF-TOF identification of proteins and maps of identified SPs were generated. Seven SPs were comparable, 27 SPs: GTP-binding/Ras-related proteins, kinases, growth factors, calcium binding proteins, phosphatase-related proteins were observed in differentiated NIE-115 cells and eight SPs of the groups mentioned above were observed in undifferentiated cells only. Switching-on/off of several individual SPs from different signaling cascades during the differentiation process is a key to understand mechanisms involved. The findings reported herein are challenging in vitro and in vivo studies to confirm a functional role for deranged SPs.
authors: Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, A 1090, Vienna, Austria.
authors: Ji-eun
authors: JE
authors: Oh
authors: Karlin Raja
authors: KR
authors: Karlmark
authors: Joo-ho
authors: JH
authors: Shin
authors: Arnold
authors: A
authors: Pollak
authors: Angelika
authors: A
authors: Freilinger
authors: Markus
authors: M
authors: Hengstschläger
authors: Gert
authors: G
authors: Lubec
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D015398:Signal Transduction / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization