Relationship between sporidesmin-induced liver injury and serum activity of gamma-glutamyltransferase in Romney lambs sired by facial eczema-resistant or control rams.
OBJECTIVE
To test whether long-term selection for resistance to facial eczema (FE) influences the relationship between liver injury score (LIS) and serum activity of gamma-glutamyltransferase (GGT) in sporidesmin-dosed sheep.


METHODS
Two groups of Romney lambs were generated for this study in 1999, out of an unselected group of ewes and sired by either selected FE-resistant (R) line (n=74 lambs) or unselected Control (C) line (n=119 lambs) rams from the Ruakura Research Centre long-term FE-selection experiment. The lambs were given a single oral dose of sporidesmin, sampled for determination of serum GGT activity 3 weeks later, and lambs that had serum GGT activity >55 IU/l (reactors) were humanely killed and assessed for LIS post mortem, 6 weeks after dosing. A second round of dosing was applied to the nonreactors, followed as above by analysis of serum GGT activity and assessment of LIS post mortem (both reactors and nonreactors).


RESULTS
There was a significant linear relationship between LIS and logeGGT, which did not differ between the 2 sire lines (LIS= -2.96 (SE 0.38) + 0.89 (SE 0.07) x loge GGT, R2=0.54, p<0.001).


CONCLUSIONS
Serum GGT is a valid indicator of LIS in lambs by sires selected for different levels of susceptibility to FE. The logeGGT-LIS relationship has not changed as a result of genetic selection for resistance to FE.
Morris
C A
CA
AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand. chris.norris@agresearch.co.nz
Smith
B L
BL
Hickey
S M
SM