A rapid multiresidual determination of type A and type B trichothecenes in wheat flour by HPLC-ESI-MS.
A new, rapid and sensitive method is reported for the multiresidual determination of type A (diacetoxyscirpenol, HT-2 toxin, T-2 toxin) and type B (nivalenol, deoxynivalenol, fusarenon X, 15-O-acetyl-4-deoxynivalenol) trichothecenes in wheat flour samples. Sample extraction was performed with acetonitrile/water mixtures. Mycosep columns were used for a fast and effective clean-up procedure. The analytes were separated by HPLC with a RP C18 column by means of a gradient elution and detected in an ESI-interfaced single quadrupole mass spectrometer. Type B and type A trichothecenes were monitored in the negative and in the positive ion mode, respectively. The method performance is reported in terms of linearity (r2 = 0.999), specificity, accuracy (recoveries from 70-120%) and precision (CV% = 5), the LOQs are in the range 10-20 microg/Kg.
Biancardi
A
A
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
Gasparini
M
M
Dall'Asta
C
C
Marchelli
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D009183:Mycotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000032:analysis; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum
D009183:Mycotoxins; D014255:Trichothecenes; C002469:fusarenon-X; C038405:nivalenol; C046760:15-acetyldeoxynivalenol; D013605:T-2 Toxin; C007262:deoxynivalenol; C012351:HT-2 toxin; C001809:diacetoxyscirpenol