[The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line].
OBJECTIVE
To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.


METHODS
Cell proliferation was assayed by MTT analysis and [3H] thymidine incorporation, after Hep-2 cell line culturing with M-CSF and M-CSFR. M-CSF concentration of surpernatant measured by a ELISA technique. Immunohistochemistry was performed on Hep-2 cell line and laryngeal carcinoma tissue.


RESULTS
The concentration of M-CSF increased after cell culturing. M-CSF can reduced the Hep-2 cell count. The action can be blocked by Anti-M-CSFR.


CONCLUSIONS
Our observations support the hypothesis that M-CSF may inhibit the growth and differentiation in Hep-2 cell line.
Ge
Pingjiang
P
Department of Otolaryngology-Head and Neck Surgery, Guangdong Province People's Hospital, Guangzhou, 510080, China.
Zhang
Baoquan
B
Zhang
Jianmin
J
Gao
Zhiqiang
Z
Kong
Qingli
Q
He
Wei
W
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000494:pharmacology*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism
D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor