Eye regeneration assay reveals an invariant functional left-right asymmetry in the early bilaterian, Dugesia japonica.
Consistent visceral asymmetry in vertebrates raises fascinating questions about the developmental mechanisms and evolutionary origin of fixed chirality of the left-right axis. One persistent controversy is whether consistently biased asymmetry is a later innovation imposed on a bilaterally symmetrical primitive body-plan, or whether asymmetry is a fundamental property predating the bilateria. The morphology of planaria suggests proximity to the origin of the bilateral body-plan, and they are commonly thought to be left-right symmetrical, as no consistent anatomical asymmetries have been described despite over a century of study of regeneration. Here, we show that D. japonica possess a consistent functional asymmetry in eye patterning defects caused by inhibition of H+/K+-ATPase activity (an ion flux mechanism recently shown to be an important early step in the asymmetry of several vertebrate embryos). Moreover, an endogenous transcript of the non-gastric H+/K+-ATPase subunit alpha is expressed in the head blastema shortly after amputation. Taken together, these data suggest that (1) left-right asymmetry is at least as old as planaria, (2) subtle functional asymmetries should be sought in other more primitive model systems that are believed to be symmetrical, and (3) symmetrical paired structures may in fact contain information about their position on the L or R side.
Nogi
Taisaku
T
Cytokine Biology Department, The Forsyth Institute, and Department of Oral and Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA.
Yuan
Yan Emily
YE
Sorocco
Debra
D
Perez-Tomas
Ricardo
R
Levin
Michael
M
D000818:Animals; D005075:Biological Evolution; D007839:Functional Laterality; D017506:H(+)-K(+)-Exchanging ATPase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016623:Ion Pumps; D009799:Ocular Physiological Phenomena; D010932:Planarians / Q000502:physiology*; D012038:Regeneration
D016623:Ion Pumps; D017506:H(+)-K(+)-Exchanging ATPase