Phenolic antioxidants from the heartwood of Acacia confusa.
In the present study, the ethanolic extracts from the heartwood of Acacia confusa, a species indigenous to Taiwan, exhibit strong antioxidant effects. Among all the fractions from ethanolic extracts of heartwood, the EtOAc soluble fraction exhibits the best antioxidant activity. The 80% 1,1-diphenyl-2-picrylhydrazyl radical inhibitory activity by the EtOAc extract was observed at a concentration of 5 mug/mL, and at the same dosage there was a similar free radical scavenging activity for (-)-ascorbic acid and (+)-catechin, both of which are well-known antioxidants. In addition, the EtOAc extract also protects PhiX174 supercoiled DNA against strand scission induced by hydroxyl radical. Furthermore, following by column chromatography and reverse-phase high-performance liquid chromatorgrapy, 10 pure phenolic compounds, including three major antioxidants (3,7,8,3',4'-pentahydroxyflavone, 7,8,3',4'-tetrahydroxy-3-methoxyflavone, and 3,4,2',3',4'-pentahydroxy-trans-chalcone) and a new flavonoid (3,7,8,3'-tetrahydroxy-4'-methoxyflavone), were isolated from the ethanolic extracts of A. confusa heartwood.
Wu
Jyh-Horng
JH
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
Tung
Yu-Tang
YT
Wang
Sheng-Yang
SY
Shyur
Lie-Fen
LF
Kuo
Yueh-Hsiung
YH
Chang
Shang-Tzen
ST
D000045:Acacia / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds; D004249:DNA Damage / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry; D017665:Hydroxyl Radical / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D014934:Wood
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl