title: Neonatal platelets from cord blood and peripheral blood.
abstract: The haemostatic system in neonates is different from that of adults, possibly contributing to an increased incidence of bleeding disorders, such as intracranial hemorrhage. In this study, we analyzed platelets from cord blood and peripheral blood, collected at three time points after delivery from 20 term and 37 preterm neonates as well as blood from 20 healthy adults. Platelet membrane glycoproteins (GP) were quantified and P-selectin expression and PAC-1 binding ability before and after stimulation with TRAP were analyzed by whole blood flow cytometry. We found no significant differences in neonatal platelets from cord blood and peripheral blood within the first 24h of life. Platelets from infants less than 30 weeks of gestation expressed lower levels of GP (33271+/-9381 vs. 44085+/-17287 for GPIIIa, P<0.05) and were less reactive than platelets from term newborns (4.3+/-3.3 vs. 20.1+/-11.8% PAC-1 positive platelets after stimulation with TRAP, P<0.05). A significantly lower level of GPIIb/IIIa expression on platelets from peripheral blood was seen in term newborns as well as preterm infants, compared to adults. There was only a partial enhancement in the degranulation ability (alpha-granules) (13.4+/-12.3 vs. 50.3+/-16.1% P-selectin positive platelets, P<0.05) and no significant increase for PAC-1 binding (13.6+/-10.9 vs. 15.3+/-5.9% PAC-1 positive platelets, P=0.8) during the first 12 days of life. In conclusion, we could demonstrate that neonatal platelet reactivity increases with gestational age.
authors: Institute of Clinical Biochemistry and Pathbiochemistry, Central Laboratory, University of Würzburg, Würzburg, Germany.
authors: A G
authors: AG
authors: Sitaru
authors: S
authors: S
authors: Holzhauer
authors: C P
authors: CP
authors: Speer
authors: D
authors: D
authors: Singer
authors: A
authors: A
authors: Obergfell
authors: U
authors: U
authors: Walter
authors: R
authors: R
authors: Grossmann
mesh_terms: D000135:Acid Phosphatase / Q000494:pharmacology; D000328:Adult; D001773:Blood Cells / Q000166:cytology*; D001792:Blood Platelets / Q000737:chemistry* / Q000502:physiology; D015550:Cell Degranulation; D054641:Dual Specificity Phosphatase 2; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes / Q000494:pharmacology; D019007:P-Selectin / Q000032:analysis; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D047928:Premature Birth; D054648:Protein Phosphatase 2; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D054648:Protein Phosphatase 2; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C517032:DUSP2 protein, human; D054641:Dual Specificity Phosphatase 2; D017027:Protein Tyrosine Phosphatases