Ascorbylated 4-hydroxy-2-nonenal as a potential biomarker of oxidative stress response.
Oxidative stress, resulting from the generation of reactive oxygen species, contributes to the development of a multitude of age-related diseases. Current methods of assessing oxidative stress levels range from the detection of lipid peroxidation products, such as F(2)-isoprostanes and malondialdehyde, to monitoring the redox status of glutathione. While useful, traditional biomarkers of oxidative stress are not without their drawbacks, including low in vitro concentrations and possible artifact formation. In the present study, we utilize liquid chromatography coupled with tandem mass spectrometry for investigation into the use of a novel compound, ascorbylated 4-hydroxy-2-nonenal, as a potential biomarker of oxidative stress.
Sowell
John
J
Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA.
Conway
Heather M
HM
Bruno
Richard S
RS
Traber
Maret G
MG
Frei
Balz
B
Stevens
Jan F
JF
D000293:Adolescent; D000328:Adult; D000447:Aldehydes / Q000032:analysis / Q000097:blood* / Q000737:chemistry*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D018384:Oxidative Stress / Q000502:physiology*; D012907:Smoking / Q000097:blood
D000447:Aldehydes; D015415:Biomarkers; C507302:ascorbyl 4-hydroxy-2-nonenal; C027576:4-hydroxy-2-nonenal; D001205:Ascorbic Acid