A fully predictive model for one-dimensional light attenuation by Chlamydomonas reinhardtii in a torus photobioreactor.
The light attenuation in a photobioreactor is determined using a fully predictive model. The optical properties were first calculated, using a data bank of the literature, from only the knowledge of pigments content, shape, and size distributions of cultivated cells which are a function of the physiology of the current species. The radiative properties of the biological turbid medium were then deduced using the exact Lorenz-Mie theory. This method is experimentally validated using a large-size integrating sphere photometer. The radiative properties are then used in a rectangular, one-dimensional two-flux model to predict radiant light attenuation in a photobioreactor, considering a quasi-collimated field of irradiance. Combination of this radiative model with the predictive determination of optical properties is finally validated by in situ measurement of attenuation profiles in a torus photobioreactor cultivating the microalgae Chlamydomonas reinhardtii, after a complete and proper characterization of the incident light flux provided by the experimental set-up.
Pottier
L
L
GEPEA Laboratory, UMR-CNRS 6144, University of Nantes, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, BP 92208, 44322 NANTES Cedex 3, France.
Pruvost
J
J
Deremetz
J
J
Cornet
J-F
JF
Legrand
J
J
Dussap
C G
CG
D000818:Animals; D019149:Bioreactors; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D003470:Culture Media; D004867:Equipment Design; D008027:Light; D008433:Mathematics; D008954:Models, Biological; D018462:Photobiology / Q000295:instrumentation; D017785:Photons; D010788:Photosynthesis; D011237:Predictive Value of Tests; D011874:Radiometry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
D003470:Culture Media