[Expression of aurora-A kinase in human lung cancer cell lines PG, A549, and NCI-H460].
OBJECTIVE
Recent researches found that Aurora-A overexpresses in various malignancies. This study was to detect the expression of Aurora-A in lung cancer cell lines PG (highly-metastatic giant cell lung cancer), A549 (lung adenocarcinoma), and NCI-H460 (large cell lung cancer) and explore its correlation to DNA content, provide a theoretical basis for screening tumor marker and molecular therapeutic target of lung cancer.


METHODS
mRNA and protein levels of Aurora-A in PG, A549, and NCI-H460 cells were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. Flow cytometry was used to analyze DNA contents in cell cycles of PG, A549, and NCI-H460 cells.


RESULTS
mRNA level of Aurora-A was 1.14 in PG cells, 1.16 in A549 cells, and 0.84 in NCI-H460 cells, respectively; protein level of Aurora-A was 8.96 in PG cells, 21.13 in A549 cells, and 6.43 in NCI-H460 cells, respectively. The proportion of cells with tetraploid DNA was 19.88% in PG cells, 14.97% in A549 cells, and 10.6% in NCI-H460 cells, respectively (P<0.01); the proportion of cells with polyploid DNA was 2.66% in PG cells, 3.59% in A549 cells, and 2.30% in NCI-H460 cells, respectively.


CONCLUSIONS
Aurora-A is overexpressed in the 3 lung cancer cell lines, but the mRNA levels are different.
Zou
Li-Juan
LJ
Department of Oncology, The Second Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, 116027, PR China.
Li
Guo-Quan
GQ
Gong
Lin-Lin
LL
Wang
Yang
Y
Jin
Wei
W
Zhao
Jin-Yao
JY
Ma
Hai-Ying
HY
Yang
Pei-Man
PM
Shao
Shu-Juan
SJ
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D064127:Aurora Kinases; D018286:Carcinoma, Giant Cell / Q000235:genetics / Q000378:metabolism*; D018287:Carcinoma, Large Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D011123:Polyploidy; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases