title: Labeling of cytochrome oxidase patches in intact flatmounts of striate cortex.
abstract: Primate striate cortex contains a prominent system of columns referred to as cytochrome oxidase (CO) patches. Studies directed at the morphology and interconnections of cells in patches would be facilitated by a method that revealed the location of patches in the intact cortex. In three adult rhesus monkeys we prepared flatmounts of striate cortex [Horton JC, Hocking DR. Intrinsic variability of ocular dominance column periodicity in normal macaque monkeys. J Neurosci 1996;16:7228-39; Sincich LC, Adams DL, Horton JC. Complete flatmounting of the macaque cerebral cortex. Vis Neurosci 2003;20:663-86]. The flattened specimens were then reacted for CO activity prior to sectioning. Transillumination of the intact cortical sheet revealed an extensive pattern of dark ovals. It was confirmed that this pattern corresponded to the CO patches by subsequently cutting tangential sections and comparing them with images from the intact block. In vitro labeling of CO patches in the intact striate cortex may prove useful for directing injections of anatomical tracers such as Lucifer Yellow or DiI into identified patch and interpatch compartments.
authors: Beckman Vision Center, University of California, 10 Koret Way, San Francisco, CA 94143-0730, USA.
authors: John R
authors: JR
authors: Economides
authors: Jonathan C
authors: JC
authors: Horton
mesh_terms: D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male; D009415:Nerve Net / Q000166:cytology* / Q000201:enzymology*; D013194:Staining and Labeling / Q000379:methods*; D014793:Visual Cortex / Q000166:cytology* / Q000201:enzymology*
chemical_list: D003576:Electron Transport Complex IV