Divalent ion-dependent swelling of tomato bushy stunt virus: a multi-approach study.
Time-resolved small-angle X-ray and neutron scattering (SAXS and SANS) in solution were used to study the swelling reaction of TBSV upon chelation of its constituent calcium at mildly basic pH. SAXS intensities comprise contribution from the protein capsid and the RNA moiety, while neutron scattering, recorded in 72% D2O, is essentially due to the protein capsid. Cryo-electron micrographs of compact and swollen virus were used to produce 3D reconstructions of the initial and final conformations of the virus at a resolution of 13 A and 19 A, respectively. While compact particles appear to be very homogeneous in size, solutions of swollen particles exhibit some size heterogeneity. A procedure has been developed to compute the SAXS pattern from the 3D reconstruction for comparison with experimental data. Cryo-electron microscopy thereby provides an invaluable starting (and ending) point for the analysis of the time-resolved swelling process using the scattering data.
Aramayo
R
R
Institut de Minéralogie et de Physique de la Matière Condensée, Université Pierre et Marie Curie UMR7590 CNRS P7 IPGP, Case Postale 115-75252 Paris Cedex 05, France.
Mérigoux
C
C
Larquet
E
E
Bron
P
P
Pérez
J
J
Dumas
C
C
Vachette
P
P
Boisset
N
N
D002413:Cations, Divalent / Q000737:chemistry; D003198:Computer Simulation; D020285:Cryoelectron Microscopy; D013286:Datura stramonium / Q000821:virology; D008958:Models, Molecular; D033363:Neutron Diffraction; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D017864:Tombusvirus / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D014965:X-Rays
D002413:Cations, Divalent