Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique.
We already found that beta-1,3-glucan polysaccharides form polymeric complexes with certain polynucleotides, but the parallel vs. anti-parallel orientation in those complexes had remained unsolved. In this paper, this controversial problem has been discussed for curdlan/oligo(dA) complexes utilizing two different energy transfer techniques. The first system consists of a combination of fluorescein-labeled curdlan and 3'-(or 5'-)tetramethyl-rhodamine (TAMRA)-labeled oligo(dA). The second system utilizes gold nanoparticles: that is, two curdlan chains were linked by a disulfide bond and after complexation with oligo(dA), the complex was immobilized on gold nanoparticles. In this system, TAMRA was attached to the 3'(or 5') end of oligo(dA) and the gold particle acted as a fluorescence quencher (energy acceptor). These experiments have led us to conclude that in the curdlan/oligo(dA) complex, parallel orientation is more favourable than anti-parallel orientation. These findings have enabled us to envision a clearer image for the complexation mode between beta-1,3-glucan polysaccharides and polynucleotides.
Numata
Munenori
M
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Koumoto
Kazuya
K
Mizu
Masami
M
Sakurai
Kazuo
K
Shinkai
Seiji
S
D019793:Fluorescein / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D047071:beta-Glucans / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D047071:beta-Glucans; C030570:oligodeoxyadenylic acid; C038459:curdlan; D019793:Fluorescein