title: Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis.
abstract: The molecular karyotype of Paranosema grylli Sokolova, Seleznev, Dolgikh et Issi, 1994, a monomorphic diplokaryotic microsporidium, comprises numerous bright and faint bands of nonstoichiometric staining intensity. Restriction analysis of chromosomal DNAs by "karyotype and restriction display" 2-D PFGE has demonstrated that the complexity of molecular karyotype of P. grylli is related to the pronounced length polymorphism of-homologous chromosomes. The background of this phenomenon is discussed in the context of ploidy state, reproductive strategy and population structure in this microsporidium. We propose that the remarkable size variation between homologous chromosomes in P. grylli may be a consequence of ectopic recombination at the chromosome extremities.
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. nosema@mail.ru
authors: Elena
authors: E
authors: Nassonova
authors: Emmanuel
authors: E
authors: Cornillot
authors: Guy
authors: G
authors: Méténier
authors: Nina
authors: N
authors: Agafonova
authors: Boris
authors: B
authors: Kudryavtsev
authors: Sergei
authors: S
authors: Skarlato
authors: Christian P
authors: CP
authors: Vivarès
mesh_terms: D000818:Animals; D016817:Apansporoblastina / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D015825:Chromosomes, Fungal / Q000235:genetics*; D017931:DNA Primers; D016521:Electrophoresis, Gel, Pulsed-Field; D005453:Fluorescence; D016681:Genome, Fungal; D006135:Gryllidae / Q000382:microbiology*; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011003:Ploidies; D012098:Reproduction / Q000502:physiology
chemical_list: D017931:DNA Primers