title: Thioredoxin gene expression in rat knee articular cartilage after full-thickness injury.
abstract: To study the expression of the antioxidative protein thioredoxin (TRX) in intact and injured articular cartilage, we examined the presence of trx mRNA in rat knee joints by in situ hybridization. Our results showed that in the intact knee, most cells, including articular cartilage chondrocytes, expressed trx mRNA. We examined joints at 1, 7, 14, and 28 days after the infliction of full-thickness cartilage injuries on distal femoral condyles. At 1 day after injury, no significant changes were observed in the wound or in trx expression pattern. However, at 7 to 28 days after injury, the wound became filled with repair tissue. Also, trx expression was detected in differentiating mesenchymal cells in the deeper zones of the wound but not in fibroblast-like cells in the upper part of the repair tissue, toward the joint cavity. This lack of TRX expression in the fibroblast-like cells may underlie the susceptibility of the repair tissue fibrocartilage to oxidative stress.
authors: Department of Orthopaedic Surgery, Shimane University School of Medicine, Izumo, Japan. kakimaru@med.shimane-u.ac.jp
authors: Hiroyuki
authors: H
authors: Kakimaru
authors: Hiroko
authors: H
authors: Kataoka
authors: Koh-ichi
authors: K
authors: Enomoto
authors: Nobuyuki
authors: N
authors: Kumahashi
authors: Akihiro
authors: A
authors: Ohira
authors: Junji
authors: J
authors: Yodoi
authors: Yuji
authors: Y
authors: Uchio
authors: Mitsuo
authors: M
authors: Ochi
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D005121:Extremities / Q000293:injuries / Q000473:pathology*; D005786:Gene Expression Regulation / Q000235:genetics*; D007596:Joints / Q000293:injuries / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D013879:Thioredoxins