title: Cytotoxic effect and electrophysiological activity of S-irniine, a synthesised isomer of the natural R-irniine, on human MRC-5 fibroblasts.
abstract: Numerous original alkaloids are present in tubers of Arisarum vulgare Targ. a species belonging to the Araceae family known in Morocco for its toxicity. Some previous works deal with the activity of these natural compounds as R-irniine. As the enantioselective total synthesis of irniine has been realised, the R- and S-forms were obtained and this last one could be tested. Thus, a study of the cytotoxicity and the electrophysiological activity of S-irniine was carried out on human MRC-5 fibroblasts. A cytotoxic effect of S-irniine at 40 microg/mL was detected on MRC-5 fibroblasts. An electrophysiological study was also carried out on the MRC-5 cells by using the patch-clamp technique and no effect of this compound at this concentration on the outward potassium current of MRC-5 fibroblasts was observed. Thus, this study showed, as it was for R-irniine, that the cytotoxicity of S-irniine was not explained by an effect on the potassium currents.
authors: Laboratory of Phytochemistry and Pharmacognosy, University Mohamed Ier, Route Sidi Maafa, B.P. 524, 60000, Oujda, Morocco.
authors: Mohammed
authors: M
authors: Lamkadem
authors: Isabelle
authors: I
authors: Schwob
authors: Jamal
authors: J
authors: Mimouni
authors: Mohammed
authors: M
authors: Aziz
authors: Abderrahime
authors: A
authors: Bouali
authors: Jacques
authors: J
authors: Rabier
authors: Robert
authors: R
authors: Fontonge
authors: Ahmed
authors: A
authors: Melhaoui
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D029064:Araceae / Q000737:chemistry*; D018929:Cell Culture Techniques; D004594:Electrophysiology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007536:Isomerism; D018408:Patch-Clamp Techniques; D011759:Pyrrolidines / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*
chemical_list: D000470:Alkaloids; D011759:Pyrrolidines; C411262:irniine