[Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC].
OBJECTIVE
To establish a method for determination of galanthamine in Lycoris radiata.


METHODS
HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.


RESULTS
Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).


CONCLUSIONS
The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.
Wu
Fang-li
FL
Zhejiang Yixin Pharmaceutical Co. LTD, Lanxi 321100, China.
Li
Ai-zhen
AZ
Mao
Hui-fang
HF
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005702:Galantamine / Q000032:analysis*; D031426:Lycoris / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D005702:Galantamine