Biases in human papillomavirus genotype prevalence assessment associated with commonly used consensus primers.
Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type-specific sensitivity may result in misprioritization of vaccine candidates.
Chan
Paul K S
PK
Department of Microbiology, School of Public Health, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin,New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk
Cheung
Tak-Hong
TH
Tam
Ann O Y
AO
Lo
Keith W K
KW
Yim
So-Fan
SF
Yu
May M Y
MM
To
Ka-Fai
KF
Wong
Yick-Fu
YF
Cheung
Jo L K
JL
Chan
Denise P C
DP
Hui
Mamie
M
Ip
Margaret
M
D019496:Cancer Vaccines; D002294:Carcinoma, Squamous Cell / Q000821:virology; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D015995:Prevalence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology
D019496:Cancer Vaccines; D017931:DNA Primers