title: Decreased 5-HT2C receptor binding in the cerebral cortex and brain stem during pancreatic regeneration in rats.
abstract: The purpose of this study was to investigate the role of central 5-HT2C receptor binding in rat model of pancreatic regeneration using 60-70% pancreatectomy. The 5-HT and 5-HT2C receptor kinetics were studied in cerebral cortex and brain stem of sham operated, 72 h pancreatectomised and 7 days pancreatectomised rats. Scatchard analysis with [3H] mesulergine in cerebral cortex showed a significant decrease (p < 0.05) in maximal binding (Bmax) without any change in Kd in 72 h pancreatectomised rats compared with sham. The decreased Bmax reversed to sham level by 7 days after pancreatectomy. In brain stem, Scatchard analysis showed a significant decrease (p < 0.01) in Bmax with a significant increase (p < 0.01) in Kd. Competition analysis in brain stem showed a shift in affinity towards a low affinity. These parameters were reversed to sham level by 7 days after pancreatectomy. Thus the results suggest that 5-HT through the 5-HT2C receptor in the brain has a functional regulatory role in the pancreatic regeneration.
authors: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, India.
authors: Valiya Veettil
authors: VV
authors: Mohanan
authors: Finla
authors: F
authors: Chathu
authors: Cheramadathikudyil S
authors: CS
authors: Paulose
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001933:Brain Stem / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004873:Ergolines / Q000378:metabolism; D010179:Pancreas / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D044405:Receptor, Serotonin, 5-HT2C / Q000378:metabolism*; D012038:Regeneration; D012701:Serotonin / Q000378:metabolism; D012702:Serotonin Antagonists / Q000378:metabolism
chemical_list: D004873:Ergolines; D044405:Receptor, Serotonin, 5-HT2C; D012702:Serotonin Antagonists; D012701:Serotonin; C031649:mesulergine