title: Isolation and characterization of murine Cds (CDP-diacylglycerol synthase) 1 and 2.
abstract: Phototransduction in Drosophila is a phosphoinositide-mediated signalling pathway. Phosphatidylinositol 4,5-bisphosphate (PIP2) plays a central role in this process, and its levels are tightly regulated. A photoreceptor-specific form of the enzyme CDP-diacylglycerol synthase (CDS), which catalyzes the formation of CDP-diacylglycerol from phosphatidic acid, is a key regulator of the amount of PIP2 available for signalling. cds mutants develop light-induced retinal degeneration. We report here the isolation and characterization of two murine genes encoding this enzyme, Cds1 and Cds2. The genes encode proteins that are 73% identical and 92% similar but exhibit very different expression patterns. Cds1 shows a very restricted expression pattern but is expressed in the inner segments of the photoreceptors whilst Cds2 shows a ubiquitous pattern of expression. Using fluorescent in situ hybridization we have mapped Cds1 and Cds2 to chromosomes 5E3 and 2G1 respectively. These are regions of synteny with the corresponding human gene localization (4q21 and 20p13). Transient transfection experiments with epitope tagged proteins have also demonstrated that both are associated with the endoplasmic reticulum.
authors: Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, UK.
authors: Suzanne L
authors: SL
authors: Inglis-Broadgate
authors: Louise
authors: L
authors: Ocaka
authors: Ruby
authors: R
authors: Banerjee
authors: Michelle
authors: M
authors: Gaasenbeek
authors: J Paul
authors: JP
authors: Chapple
authors: Michael E
authors: ME
authors: Cheetham
authors: Brian J
authors: BJ
authors: Clark
authors: David M
authors: DM
authors: Hunt
authors: Stephanie
authors: S
authors: Halford
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D002798:Diacylglycerol Cholinephosphotransferase / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005091:Exons; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D002798:Diacylglycerol Cholinephosphotransferase