Six novel Arthrobacter species isolated from deteriorated mural paintings.
A group of 21 bacterial strains was isolated from samples of biofilm formation in the Servilia tomb (necropolis of Carmona, Spain) and the Saint-Catherine chapel (castle at Herberstein, Austria). A polyphasic taxonomic study of these isolates, including morphological, biochemical and chemotaxonomic characterization, rep-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA base ratio and DNA-DNA relatedness studies, allocated them to the genus Arthrobacter. The isolates represent six novel species, for which the names Arthrobacter castelli sp. nov., Arthrobacter monumenti sp. nov., Arthrobacter parietis sp. nov., Arthrobacter pigmenti sp. nov., Arthrobacter tecti sp. nov. and Arthrobacter tumbae sp. nov. are proposed. The respective type strains are LMG 22283(T) (=DSM 16402(T)), LMG 19502(T) (=DSM 16405(T)), LMG 22281(T) (=DSM 16404(T)), LMG 22284(T) (=DSM 16403(T)), LMG 22282(T) (=DSM 16407(T)) and LMG 19501(T) (=DSM 16406(T)).
Heyrman
Jeroen
J
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Verbeeren
Jens
J
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Swings
Jean
J
BCCM/LMG Bacteria Collection, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.|Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
De Vos
Paul
P
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
D001173:Arthrobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001317:Austria; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms / Q000254:growth & development*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010151:Paintings; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S