title: Evolution and expression of translationally controlled tumour protein (TCTP) of fish.
abstract: Translationally Controlled Tumour Protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans. In order to better understand its functions in non-mammalians, cDNA encoding full-length TCTP has been isolated and characterized from a teleost fish, Labeo rohita (rohu). Encoded by a 1043 nucleotide mRNA, rohu TCTP consists of 171 amino acids and is expressed in all organs, except in brain. Secondary structure of fish TCTP mRNAs shows that they could be potential substrates for RNA specific protein kinase PKR. The three-dimensional structure of rohu TCTP has been determined, as the first metazoan model of this protein. The conservational and phylogenic clustering of plant and animal TCTP sequences is consistent with the eukaryotic classification, and is suggestive of early origin for the TCTP ortholog in eukaryote evolution, as early as 1.0 x 10(9) years ago. Despite significant conservation, meticulous sequence analysis reveals striking differences that suggest possible new functions for TCTP.
authors: Department of Genetics, Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625021, India. thayanithy.venugopal@umassmed.edu
authors: Venugopal
authors: V
authors: Thayanithy
authors: Thayanithy
authors: T
authors: Venugopal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D019143:Evolution, Molecular; D005399:Fishes; D005786:Gene Expression Regulation; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D010961:Plasmodium / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012568:Schizosaccharomyces / Q000378:metabolism; D020539:Sequence Analysis, Protein; D000090324:Tumor Protein, Translationally-Controlled 1; D019892:eIF-2 Kinase / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D012333:RNA, Messenger; D000090324:Tumor Protein, Translationally-Controlled 1; D019892:eIF-2 Kinase