Application of accelerated solvent extraction followed by gel performance chromatography and high-performance liquid chromatography for the determination of polycyclic aromatic hydrocarbons in mussel tissue.
Accelerated solvent extraction (ASE) has been evaluated as a fast alternative to methanolic saponification for the extraction of 12 polycyclic aromatic hydrocarbons (PAHs) from mussel tissue. Several solvent systems and different operating conditions were investigated. The mixture dichloromethane-acetone (1:1, v/v) gave the best recoveries at 125 degrees C and 1500 psi, in a total time of 10 min. No yield difference was found between freeze-drying (Fd) or drying the wet mussel with diatomaceous earth (Ded) prior to extraction. The ASE method was validated using the standard reference material SRM 2977, a freeze-dried mussel tissue with naturally present organic contaminants. The performance characteristics of the ASE method (trueness: 70-110%; precision: 4-14% and limit of quantification (LOQ): 0.1-0.25 microg/kg) meet the criteria established by the European Union for quantitative methods of analysis for official control of organic residues and contaminants. ASE provides a 24 times faster extraction than MSE and reduces 12 times the volume of solvent required.
Yusà
V
V
Public Health Laboratory of Valencia, Generalitat Valenciana, C/Micer Mascó 31, 46010-Valencia, Spain. yusa_vic@gva.es
Pardo
O
O
Martí
P
P
Pastor
A
A
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D005612:Freeze Drying; D006358:Hot Temperature; D009930:Organic Chemicals / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012997:Solvents; D013997:Time Factors; D014020:Tissue Extracts / Q000032:analysis
D004785:Environmental Pollutants; D009930:Organic Chemicals; D011084:Polycyclic Aromatic Hydrocarbons; C504352:SRM 2977; D012997:Solvents; D014020:Tissue Extracts