A collaborative study of the EDNAP group regarding Y-chromosome binary polymorphism analysis.
A collaborative study was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the performance of Y-chromosome binary polymorphism analysis in different European laboratories. Four blood samples were sent to the laboratories, to be analysed for 11 Y-chromosome single nucleotide polymorphisms (SNPs): SRY-1532, M40, M35, M213, M9, 92R7, M17, P25, M18, M153 and M167. All the labs were also asked to submit a population study including these markers. All participating laboratories reported the same results, indicating the reproducibility and robustness of Y-chromosome SNP typing. A total of 535 samples from six different European populations were also analysed. In Galicia (NW Spain) and Belgium, the most frequent haplogroup was R1b*(xR1b1,R1b3df). Haplogroup F*(xK) is one of the most frequent in Austria and Denmark, while the lowest frequency appear in Belgium. Haplogroup frequencies found in this collaborative study were compared with previously published European Y-chromosome haplogroup data.
Brion
María
M
Institute of Legal Medicine, University of Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain. brioniml@usc.es
Dupuy
Berit M
BM
Heinrich
Marielle
M
Hohoff
Carsten
C
Hoste
Bernardette
B
Ludes
Bertrand
B
Mevag
Bente
B
Morling
Niels
N
Niederstätter
Harald
H
Parson
Walther
W
Sanchez
Juan
J
Bender
Klaus
K
Siebert
Nathalie
N
Thacker
Catherine
C
Vide
Conceiçao
C
Carracedo
Angel
A
D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D005060:Europe; D005819:Genetic Markers; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
D005819:Genetic Markers