title: L1 CAM expression is increased surrounding the lesion site in rats with complete spinal cord transection as neonates.
abstract: L1 is a cell adhesion molecule associated with axonal outgrowth, fasciculation, and guidance during development and injury. In this study, we examined the long-term effects of spinal cord injury with and without exercise on the re-expression of L1 throughout the rat spinal cord. Spinal cords from control rats were compared to those from rats receiving complete mid-thoracic spinal cord transections at postnatal day 5, daily treadmill step training for up to 8 weeks, or both transection and step training. Three months after spinal cord transection, we observed substantially higher levels of L1 expression by both Western blot analysis and immunocytochemistry in rats with and without step training. Higher expression levels of L1 were seen in the dorsal gray matter and in the dorsal lateral funiculus both above and below the lesion site. In addition, L1 was re-expressed on the descending fibers of the corticospinal tract above the lesion. L1-labeled axons also expressed GAP-43, a protein associated with axon outgrowth and regeneration. Treadmill step training had no effect on L1 expression in either control or transected rats despite the fact that spinal transected rats displayed improved stepping patterns indicative of spinal learning. Thus, spinal cord transection at an early age induced substantial L1 expression on axons near the lesion site, but was not additionally augmented by exercise.
authors: Department of Physiological Science, UCLA, Los Angeles, CA 90095-1606, USA.
authors: M D
authors: MD
authors: Kubasak
authors: E
authors: E
authors: Hedlund
authors: R R
authors: RR
authors: Roy
authors: E M
authors: EM
authors: Carpenter
authors: V R
authors: VR
authors: Edgerton
authors: P E
authors: PE
authors: Phelps
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005080:Exercise Test; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D009434:Neural Pathways / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011712:Pyramidal Tracts / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000254:growth & development* / Q000378:metabolism / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000503:physiopathology / Q000534:rehabilitation*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015415:Biomarkers; D019922:GAP-43 Protein; D039842:Neural Cell Adhesion Molecule L1