title: Brain-derived neurotrophic factor selectively enhances allergen-specific IgE production.
abstract: We studied the effect of brain-derived neurotrophic factor (BDNF) on in vitro Japanese cedar pollen (JCP)-specific IgE production by mononuclear cells from atopic keratoconjunctivitis patients with JCP allergy. BDNF enhanced JCP-specific IgE production in a dose-dependent fashion in cultures of mononuclear cells stimulated with JCP, and maximal enhancement was achieved at 10 ng/ml. In contrast, BDNF had no effect on JCP-specific IgA or IgG4 production. On the other hand, other neurotrophins, NGF, NT-3, or NGF failed to enhance JCP-specific IgE production. Moreover, anti-BDNF mAb specifically blocked BDNF-induced enhancement of JCP-specific IgE production. Study for cytokine production revealed that BDNF decreased production of Th1 cytokines, IFN-gamma and IL-12, while it had no effect on production of TH2 cytokines, IL-4, IL-10 and IL-13, in cultures of mononuclear cells stimulated with JCP. These results indicate that BDNF relatively skews cytokine pattern toward Th2 type. Collectively, BDNF may increase allergen-specific IgE production, which may in turn aggravate allergic symptoms.
authors: Department of Allergy, Satou Hospital, 65-1, Yabuhigashimachi, Hirakata-City, Osaka Prefecture 573-1124, Japan. kimata@misugikai.jp
authors: H
authors: H
authors: Kimata
mesh_terms: D000485:Allergens / Q000276:immunology; D000918:Antibody Specificity; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D032485:Cryptomeria / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004347:Drug Interactions / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D011058:Pollen / Q000276:immunology; D013666:Tears / Q000276:immunology
chemical_list: D000485:Allergens; D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D018664:Interleukin-12; D007073:Immunoglobulin E; D007371:Interferon-gamma