title: Reliable characterization of coffee bean aroma profiles by automated headspace solid phase microextraction-gas chromatography-mass spectrometry with the support of a dual-filter mass spectra library.
abstract: This investigation is based on the automated solid phase microextraction GC-MS analysis of the volatile fraction of a variety of coffee bean matrices. Volatile analytes were extracted by headspace (HS)-SPME which was achieved with the support of automated instrumentation. The research was directed towards various important aspects relating to coffee aroma analysis: monitoring of the volatile fraction formation during roasting; chromatographic differentiation of the two main coffee species (Arabica and Robusta) and of a single species from different geographical origins; evaluation of the influence of specific industrial treatments prior to roasting. Reliable peak assignment was carried out through the use of a recently laboratory-constructed "flavour and fragrance" library and a dual-filter MS spectral search procedure. Further emphasis was placed on the automated SPME instrumentation and on its ability to supply highly repeatable chromatographic data.
authors: Dipartimento Farmaco-chimico, Università degli Studi di Messina, Viale Annunziata, 98168 Messina, Italy.
authors: Luigi
authors: L
authors: Mondello
authors: Rosaria
authors: R
authors: Costa
authors: Peter Quinto
authors: PQ
authors: Tranchida
authors: Paola
authors: P
authors: Dugo
authors: Maria
authors: M
authors: Lo Presti
authors: Saverio
authors: S
authors: Festa
authors: Alessia
authors: A
authors: Fazio
authors: Giovanni
authors: G
authors: Dugo
mesh_terms: D003069:Coffee / Q000737:chemistry* / Q000145:classification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D003069:Coffee