Characterization of prolidase activity in erythrocytes from a patient with prolidase deficiency: comparison with prolidase I and II purified from normal human erythrocytes.
OBJECTIVE
The purpose of this study was to investigate the effect of various amino acids and their metabolites on the activities of prolidase I and II from human erythrocytes compared to those in a patient with prolidase deficiency.


METHODS
Prolidase I and II from human erythrocytes were purified by using column chromatography. Prolidase activity against various iminodipeptides was determined by spectrophotometry using Chinard's method.


RESULTS
The activities of prolidase I and II against glycylproline and methionylproline were enhanced by glycine, L- and D-isoforms of alanine and serine and D-isoforms of valine, leucine and isoleucine. L-isoforms of branched amino acids inhibited the activity of prolidase I. On the other hand, the activity of prolidase II was enhanced by all of these L-branched amino acids. The patient's prolidase activity was also enhanced by all the L- and D-branched amino acids.


CONCLUSIONS
The activities of prolidase I and II against various iminodipeptides were prominently enhanced by glycine, but the effect of L-valine differed between the two enzymes. Enzymatic properties of the patient's prolidase were essentially the same as those of prolidase II.
Liu
Gang
G
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi 783-8505, Japan.
Nakayama
Kazuko
K
Sagara
Yasuhiro
Y
Awata
Shiro
S
Yamashita
Koichi
K
Manabe
Masanobu
M
Kodama
Hiroyuki
H
D000596:Amino Acids / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004150:Dipeptidases / Q000097:blood* / Q000172:deficiency* / Q000302:isolation & purification; D004151:Dipeptides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D017895:Manganese Compounds / Q000494:pharmacology; D013237:Stereoisomerism; D014633:Valine / Q000494:pharmacology
D000596:Amino Acids; D002712:Chlorides; D004151:Dipeptides; D017895:Manganese Compounds; C015248:glycylproline; D004150:Dipeptidases; C022449:proline dipeptidase; D014633:Valine; C025340:manganese chloride