Aerosolized hyaluronan limits airspace enlargement in a mouse model of cigarette smoke-induced pulmonary emphysema.
This study was designed to determine if aerosolized hyaluronan (HA) could prevent airspace enlargement and elastic fiber injury in a mouse model of cigarette smoke-induced pulmonary emphysema. Compared to untreated/smoked controls, HA-treated animals showed statistically significant reductions in mean linear intercept (54 versus 65 microm; P < .001) and elastic fiber breakdown products (desmosine and isodesmosine) in bronchoalveolar lavage fluid (0.3 versus 7.0 ng/mL; P < .05). As in previous studies, the aerosolized HA showed preferential binding to elastic fibers, suggesting that it may protect them from injury. These findings support further investigation of the potential use of HA as a treatment for pulmonary emphysema.
Cantor
Jerome O
JO
St. John's University School of Pharmacy and Allied Health Sciences, Jamaica, New York 11439, USA. cantorj@stjohns.edu
Cerreta
Joseph M
JM
Ochoa
Marcos
M
Ma
Shuren
S
Chow
Tony
T
Grunig
Gabriele
G
Turino
Gerard M
GM
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D003895:Desmosine / Q000378:metabolism; D004195:Disease Models, Animal; D004547:Elastic Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D007524:Isodesmosine / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D012907:Smoking / Q000009:adverse effects*
D000276:Adjuvants, Immunologic; D000336:Aerosols; D003895:Desmosine; D006820:Hyaluronic Acid; D007524:Isodesmosine