Downstream processing of MDCK cell-derived equine influenza virus.
A microcarrier-based process was used to produce equine influenza virus (A/Equi 2 (H3N8), Newmarket 1/93) in Madin Darby Canine kidney (MDCK) cells. The virus was purified in a sequence of downstream processing steps comprising of depth filtration, inactivation, ultrafiltration (UF) and gel filtration. In the ultrafiltration step, the hemagglutinin (HA) was recovered to 100%. A high increase of neuraminidase (NA) activity indicated the removal of some inhibitory compounds during this step. At the same time, the level of contaminating proteins and DNA was reduced by more than 88%. In the subsequent size exclusion chromatography (Sepharose CL 2B), the recovery of HA and NA in the "virus peak" was 37.8 and 59.8%, respectively compared to the concentrated feed material. Inconsistencies in the overall mass balance for HA and NA (70.0 and 69.2%) during gel filtration indicated non-specific interactions of the inactivated virus to the gel matrix which is supported by a HA recovery of about 50% in shake flask experiments performed as a control. Overall 35.8% of HA and 291.6% of NA were recovered. More than 95.7% of the host cell proteins and 98.7% of the host cell DNA were removed during downstream processing.
Nayak
Deba Prasad
DP
Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, D-39106 Magdeburg, Germany.
Lehmann
Sylvia
S
Reichl
Udo
U
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006389:Hemagglutinins, Viral / Q000032:analysis; D009980:Influenza A virus / Q000254:growth & development / Q000302:isolation & purification*; D015203:Reproducibility of Results; D014462:Ultrafiltration; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation / Q000379:methods
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D014764:Viral Proteins