title: Quantitative unspun-urine microscopy as a quick, reliable examination for bacteriuria.
abstract: The diagnosis and treatment of urinary infection are often delayed, causing renal damage, largely because of the unavailability of quick, accurate, diagnostic examinations. Three hundred and twenty-five urine samples from 130 patients were examined for significant bacteriuria using the standard culture method. The urine samples were also examined using the Gram-stain method and quantitative unspun-urine microscopy. When particles could not be distinguished definitely as bacilli by quantitative microscopy, the unspun urine was examined on a slide glass using oil-immersion microscopy at x 1000 magnification. Significant bacteriuria in 37 urine samples was detected by bacterial culture. Using quantitative microscopy, rods were found in 30, cocci in a chain in 3, and indefinite particles in 44 samples. In the 44 indefinite samples, oil-immersion microscopy was able to distinguish rods in one, cocci in a chain in one, cocci in a cluster in two, and negative in 40, which were confirmed by culture as rods, streptococci, staphylococci, and negative, respectively. The quantitative microscopy method was similarly reliable (94.6% sensitivity, 99.3% specificity) for diagnosis of significant bacteriuria when compared with the Gram-stain method (89.2% sensitivity, 98.6% specificity). Quantitative unspun-urine microscopy, confirmed by oil-immersion, is a quick, reliable method for diagnosis of significant bacteriuria, and is considered to be useful for early diagnosis of urinary infection.
authors: Department of Pediatrics, University of Fukui, Faculty of Medical Sciences, Fukui, Japan. hiraoka@fmsrsa.fukui-med.ac.jp
authors: M
authors: M
authors: Hiraoka
authors: Y
authors: Y
authors: Hida
authors: Y
authors: Y
authors: Mori
authors: H
authors: H
authors: Tsukahara
authors: Y
authors: Y
authors: Ohshima
authors: H
authors: H
authors: Yoshida
authors: M
authors: M
authors: Mayumi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001437:Bacteriuria / Q000175:diagnosis* / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005840:Gentian Violet; D006801:Humans; D007223:Infant; D008297:Male; D008828:Microbiological Techniques; D008853:Microscopy; D008875:Middle Aged; D010619:Phenazines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000145:classification / Q000166:cytology / Q000302:isolation & purification; D013291:Streptococcus / Q000145:classification / Q000166:cytology / Q000302:isolation & purification; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet