Actin-based motility of endosomes is linked to the polar tip growth of root hairs.
Plant tip growth has been recognized as an actin-based cellular process requiring targeted exocytosis and compensatory endocytosis to occur at the growth cone. However, the identity of subcellular compartments involved in polarized membrane trafficking pathways remains enigmatic in plants. Here we characterize endosomal compartments in tip-growing root hair cells. We demonstrate their presence at the growing tip and differential distribution upon cessation of tip growth. We also show that both the presence of endosomes as well as their rapid movements within the tip region depends on an intact actin cytoskeleton and involves actin polymerization. In conclusion, actin-propelled endosomal motility is tightly linked to the polar tip growth of root hairs.
Voigt
Boris
B
Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany.
Timmers
Antonius C J
AC
Samaj
Jozef
J
Hlavacka
Andrej
A
Ueda
Takashi
T
Preuss
Mary
M
Nielsen
Erik
E
Mathur
Jaideep
J
Emans
Neil
N
Stenmark
Harald
H
Nakano
Akihiko
A
Baluska
Frantisek
F
Menzel
Diedrik
D
D000199:Actins / Q000235:genetics / Q000502:physiology*; D000730:Androstadienes / Q000494:pharmacology; D017360:Arabidopsis; D020126:Brefeldin A / Q000494:pharmacology; D047630:Depsipeptides / Q000494:pharmacology; D011992:Endosomes / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008164:Luminescent Proteins; D046913:Medicago truncatula; D018613:Microscopy, Confocal; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000077191:Wortmannin
D000199:Actins; D000730:Androstadienes; D047630:Depsipeptides; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C413663:fluorescent protein 583; C057531:jasplakinolide; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; D000077191:Wortmannin