Molecular cytogenetics analysis with whole chromosome paint probes of sperm nuclei from a (13;15) Robertsonian translocation carrier.
Meiotic segregation of a Robertsonian translocation (13;15) was assessed in sperm nuclei using dual-color fluorescent in situ hybridization (FISH) with whole-chromosome paint probes. Most spermatozoa in the (13;15) translocation carrier resulted from alternate segregation. Although an increased frequency of unbalanced gametes was observed, spontaneous pregnancy led to the birth of a boy with a normal karyotype.
Rives
Nathalie
N
Laboratoire de Biologie de la Reproduction-CECOS, CHU Charles Nicolle, Rouen, France. nathalie.rives@chu-rouen.fr.
Ravel
Célia
C
Service d'Histologie, Biologie de la Reproduction et Cytogénétique, Hôpital Tenon, Paris, France.
Duchesne
Véronique
V
Laboratoire de Biologie de la Reproduction-CECOS, CHU Charles Nicolle, Rouen, France.
Siffroi
Jean-Pierre
JP
Service d'Histologie, Biologie de la Reproduction et Cytogénétique, Hôpital Tenon, Paris, France.
Mousset-Siméon
Nathalie
N
Laboratoire de Biologie de la Reproduction-CECOS, CHU Charles Nicolle, Rouen, France.
Macé
Bertrand
B
Laboratoire de Biologie de la Reproduction-CECOS, CHU Charles Nicolle, Rouen, France.
D020223:Chromosome Painting; D020090:Chromosome Segregation / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods; D005817:Genetic Counseling; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000737:chemistry*; D014178:Translocation, Genetic / Q000235:genetics*