title: Per-follicle measurements indicate that anti-müllerian hormone secretion is modulated by the extent of follicular development and luteinization and may reflect qualitatively the ovarian follicular status.
abstract: OBJECTIVE
  To investigate the possible influence of follicular maturation and luteinization on anti-müllerian hormone (AMH) secretion and the relationship between per-follicle AMH levels, ovarian follicular status, and responsiveness to controlled ovarian hyperstimulation (COH).
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital in France.
  
  
  METHODS
  Thirty seven in vitro fertilization/embryo transfer candidates undergoing COH.
  
  
  METHODS
  On the day of oocyte retrieval, serum samples and follicular fluids from two small (8-12 mm in diameter) and two large (16-20 mm in diameter) follicles were collected for AMH, E2, and progesterone (P4) measurements.
  
  
  METHODS
  Per-follicle AMH levels.
  
  
  RESULTS
  Small follicles secreted AMH levels that were approximately three times as high as large follicles. Follicular fluid AMH and P4 levels were negatively correlated to each other both in small and large follicles. Per-follicle AMH levels in both follicular classes were positively correlated with antral follicle count on cycle day 3 before COH and with growing follicle (> or =12 mm) count and oocytes retrieved, but negatively correlated with FSH requirement.
  
  
  CONCLUSIONS
  Both final follicular maturation and luteinization interfere with granulosa cell AMH production. The relationship between intrafollicular AMH content, the surrounding follicular status, and ovarian response to COH indicates that peripheral AMH levels reflect not only follicle count but also per-follicle AMH production.
authors: Department of Obstetrics and Gynecology and Reproductive Medicine, Clamart, France. renato.fanchin@abc.ap-hop-paris.fr
authors: Renato
authors: R
authors: Fanchin
authors: Nabil
authors: N
authors: Louafi
authors: Daniel H
authors: DH
authors: Méndez Lozano
authors: Nelly
authors: N
authors: Frydman
authors: René
authors: R
authors: Frydman
authors: Joëlle
authors: J
authors: Taieb
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D034461:Luteinization / Q000378:metabolism*; D006080:Ovarian Follicle / Q000254:growth & development* / Q000378:metabolism*; D011446:Prospective Studies; D013735:Testicular Hormones / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone