Gadolinium-rhodamine nanoparticles for cell labeling and tracking via magnetic resonance and optical imaging.
A novel dual-labeled nanoparticle for use in labeling and tracking cells in vivo is described. We report the construction and characterization of these gadolinium-rhodamine nanoparticles. These particles are constructed from lipid monomers with diacetylene bonds that are sonicated and photolyzed to form polymerized nanoparticles. Cells are efficiently labeled with these nanoparticles. We have inoculated labeled tumor cells subcutaneouosly into the flanks of C3H mice and have been able to image these labeled tumor cells via MRI and optical imaging. Furthermore, the labeled tumor cells can be visualized via fluorescent microscopy after tissue biopsy. Our results suggest that these nanoparticles could be used to track cells in vivo. This basic platform can be modified with different fluorophores and targeting agents for studying metastisic cell, stem cell, and immune cell trafficking among other applications.
Vuu
Kien
K
Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, 4000 Jones Bridge Road, Chevy Chase, Maryland 20815-6789, USA.
Xie
Jianwu
J
McDonald
Michael A
MA
Bernardo
Marcelino
M
Hunter
Finie
F
Zhang
Yantian
Y
Li
King
K
Bednarski
Mark
M
Guccione
Samira
S
D000818:Animals; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008809:Mice, Inbred C3H; D036103:Nanotechnology; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D055095:Optics and Photonics; D010316:Particle Size; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D012235:Rhodamines; D005682:Gadolinium