[Detection rate of human papillomavirus-16 in esophageal squamous cell carcinoma from different Chinese populations].
OBJECTIVE
Anyang in Henan Province of China is a hyperendemic area of esophageal cancer. The infection of human papillomavirus (HPV) is thought as an important pathogenesis of esophageal cancer in Anyang. This study was to detect infection rate and level of HPV-16 in esophageal squamous cell carcinoma (ESCC) patients from 3 different Chinese areas, and investigate its relationship with the pathogenesis of ESCC.


METHODS
Infection status of HPV-16 in 119 ESCC specimens (43 collected from Anyang, 43 from Beijing, and the rest 33 from Mongolia nationality of Inner Mongolia) was detected by in situ hybridization (ISH) technique with digoxin-labeled HPV-16 E6 probe.


RESULTS
HPV16 infection rates were 81.4%, 69.8%, and 63.6% in the specimens from Anyang, Beijing, and Inner Mongolia, respectively. Infection level of HPV-16 was significantly higher in Anyang group than in Beijing group (H=3.91, P<0.05) and Inner Mongolia group(H=4.22,P<0.05). There was no significant difference between the latter 2 groups. Furthermore, the proportion of strong expression of HPV16 (++ and +++) was significantly higher in Anyang group than in the other 2 groups(H=3.95, P<0.05).


CONCLUSIONS
HPV-16 infection rate is high in the esophageal specimens from the 3 different areas. Infection status of HPV16 is serious in Anyang.
Zhu
Lin-Zhong
LZ
Department of Genetics, Beijing Institute of Cancer Research/Beijing Cancer Hospital/ School of Clinical Oncology, Peking University, Beijing, 100034, PR China.
Su
Xiu-Lan
XL
Chen
Ke-Neng
KN
Yang
Ren-Jie
RJ
Xing
Hai-Ping
HP
Cui
Jian-Guo
JG
Ke
Yang
Y
D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D002681:China / Q000453:epidemiology; D015344:DNA Probes, HPV / Q000302:isolation & purification*; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000821:virology*; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology
D015344:DNA Probes, HPV