Mesenchymal stem cells can be obtained from the human saphena vein.
Mesenchymal stem cells (MSC) can be isolated from many sites adults and the fetus. Cells with osteoblastic, chondrogenic, leiomiogenic and stromogenic potentials have been obtained from the bovine artery wall, and we now show that MSC can be isolated also from the adult human vein wall. Cells detached from internal surface of the saphenous vein are cultured in vitro for 2-3 weeks and replated weekly. The culture forms a semi-confluent layer of spindle-shaped cells that are CD13(+), CD29(+), CD44(+), CD34(-), CD45(-), CD14(-), CD133(-), CD31(-), CD33(-), CD54(+), CD106(-), CD90(+), KDR(-), cadherin-5-, HLA class I(+) and HLA-DR- and differentiate in vitro into osteoblasts, chondrocytes and adipocytes. Gene expression, when compared with seven other normal tissues, shows strong similarity with MSC obtained from other sources. Three genes more expressed in saphenous MSC than in the other two MSC are related to angiogenesis, and the expression of two of them is shared by endothelial cells. These results demonstrate that the human vein wall contains mesenchymal cells with morphologic features, immunophenotypic markers, gene expression profile and differentiation potential that are similar to MSC obtained from the bone marrow and from the umbilical vein.
Covas
Dimas T
DT
Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Piccinato
Carlos E
CE
Orellana
Maristela D
MD
Siufi
Jorge L C
JL
Silva
Wilson A
WA
Proto-Siqueira
Rodrigo
R
Rizzatti
Edgar G
EG
Neder
Luciano
L
Silva
Ane R L
AR
Rocha
Vanderson
V
Zago
Marco A
MA
D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D019070:Cell Lineage; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012501:Saphenous Vein / Q000166:cytology*; D014648:Varicose Veins
D015676:Osteonectin; D012333:RNA, Messenger