[Effect of intestinal bacteria on metabolism of ophiopognin saponin D' in rats].
OBJECTIVE
To explore the metabolic transformation and the absorbed metabolites of ophiopognin saponin D' (OD') given orally in rats.


METHODS
The contents of both original OD' and its metabolites were detected by means of HPLC-ELSD and the metabolites of OD' in blood and urine were measured by use of TLC and HPLC-MS in vivo.


RESULTS
OD' could be metabolized by intestinal bacteria in rats. The content of diosgenin, one of the metabolites, increased gradually as the time passed.


CONCLUSIONS
OD' can be metabolized in intestine of rat and its metabolite, diosgenin, was absorbed in blood of rat.
Shen
Lan
L
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Xu
De-Sheng
DS
Feng
Yi
Y
Li
Xiao
X
Wu
Xiao-Ru
XR
D000284:Administration, Oral; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D004144:Diosgenin / Q000097:blood / Q000378:metabolism* / Q000652:urine; D007422:Intestines / Q000382:microbiology*; D008297:Male; D031441:Ophiopogon / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*
D012503:Saponins; D004144:Diosgenin