Ultrathin functional star PEG coatings for DNA microarrays.
In this study, star PEG coatings on glass substrates have been used as support material for oligonucleotide microarrays. These coatings are prepared from solutions of six armed star shaped prepolymers that carry reactive isocyanate endgroups. As described earlier, such films prevent the adsorption of proteins and the adhesion of cells but can easily be functionalized for specific biological recognition. Here we used the high functionality of these coatings for the covalent immobilization of amino terminated 20mer oligonucleotides, both by microcontact printing and spotting techniques. The permanent immobilization of fluorescently labeled DNA as well as hybridization of 20mer oligonucleotides have been monitored by fluorescence microscopy. The hybridization efficiency as determined by fluorescence intensity varied from 30% to 80% depending on the way of layer preparation. The direct spotting without additional activation and blocking steps of the surface demonstrates the potential of star PEG coatings as ultrathin surface modification for microarrays.
Ameringer
Thomas
T
Deutsches Wollforschungsinstitut an der RWTH Aachen e.V., Pauwelsstrasse 8, 52074 Aachen, Germany.
Hinz
Michael
M
Mourran
Claudia
C
Seliger
Hartmut
H
Groll
Juergen
J
Moeller
Martin
M
D001483:Base Sequence; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011092:Polyethylene Glycols / Q000737:chemistry*
D011092:Polyethylene Glycols