title: Polyelectrolyte complex micelles composed of c-raf antisense oligodeoxynucleotide-poly(ethylene glycol) conjugate and poly(ethylenimine): effect of systemic administration on tumor growth.
abstract: An antisense oligodeoxynucleotide (ODN) delivery system based on polyelectrolyte complex (PEC) micelles composed of an ODN-poly(ethylene glycol) (PEG) conjugate and polyethylenimine (PEI) was demonstrated. The PEC micelles having a core/shell structure were spontaneously formed in an aqueous solution by ionic interactions between ODN part in the conjugate and PEI. The ODN/PEI polyelectrolyte complex formed an inner core while PEG chains surrounded it as a shell. The morphology of the micelles was visualized as a separate sphere by atomic force microscopy (AFM). When the micelles containing a c-raf antisense ODN were intravenously administered into tumor-bearing nude mice, significant antitumor activities against human lung cancer were observed. The intravenously injected micelles also showed significantly higher accumulation level in the solid tumor region compared to that of naked ODN.
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea (South.
authors: Ji Hoon
authors: JH
authors: Jeong
authors: Sun Hwa
authors: SH
authors: Kim
authors: Sung Wan
authors: SW
authors: Kim
authors: Tae Gwan
authors: TG
authors: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004573:Electrolytes / Q000737:chemistry*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D018625:Microscopy, Atomic Force; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D019908:Proto-Oncogene Proteins c-raf / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004573:Electrolytes; D008823:Micelles; D016376:Oligonucleotides, Antisense; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D019908:Proto-Oncogene Proteins c-raf