title: cDNA cloning, genomic structure and expression analysis of the goose (Anser cygnoides) MHC class I gene.
abstract: To provide data for studies on avian disease resistance, goose MHC class I cDNA (Ancy-MHC I) was cloned from a goose cDNA library, it's genomic structure and expression analysis were investigated. The mature peptides of Ancy-MHC I cDNA encoded 333 amino acids. The genomic organization is composed of eight exons and seven introns. Based on the genetic distance, six Ancy-MHC I genes from six individuals can be classified into four lineages. A total of nineteen amino acid positions in peptide-binding domain showed high scores by Wu-kabat index analysis. The Ancy-MHC I amino acid sequence displayed seven critical HLA-A2 amino acids that bind with antigen polypeptides, and have an 85.4-98.9% amino acid homology with each genes, and a 59.8-66.0% amino acid homology with chicken MHC class I. Expression analyses using Q-RT-PCR to detect the tissue-specific expression of Ancy-MHC I mRNA in an adult goose. The result appeared that Ancy-MHC I cDNA was expressed in the liver, spleen, intestine, kidney, lung, pancreas, heart, brain, and skin. The phylogenetic tree appears to branch in an order consistent with accepted evolutionary pathways.
authors: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China. xiachun@cau.edu.cn
authors: Chun
authors: C
authors: Xia
authors: Tuanjun
authors: T
authors: Hu
authors: Tianyao
authors: T
authors: Yang
authors: Li
authors: L
authors: Wang
authors: Guangxian
authors: G
authors: Xu
authors: Changyou
authors: C
authors: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005777:Geese / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D015723:Gene Library; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I