Expression of NTPDase1 and caveolins in human cardiovascular disease.
Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.
Kittel
Agnes
A
Department of Pharmacology, Hungarian Academy of Sciences, Institute of Experimental Medicine, P.O. Box 67, 1450 Budapest, Hungary. kittel@koki.hu
Kiss
Anna L
AL
Müllner
Nándor
N
Matkó
Ida
I
Sperlágh
Beáta
B
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000378:metabolism*; D022461:Caveolins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006868:Hydrolysis; D007150:Immunohistochemistry; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D013997:Time Factors
D015703:Antigens, CD; D022461:Caveolins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D001081:Apyrase; C067869:CD39 antigen