Characterization of monoclonal antibody specific to the Z39Ig protein, a member of immunoglobulin superfamily.
Z39Ig, a recently identified immunoglobulin (Ig) superfamily member, is localized in the pericentromeric region of human chromosome X and detectable in all human tissue, but it is predominantly expressed in fetal human tissues as well as in adult lungs and placenta. In the present study, we generated a monoclonal antibody against Z39Ig protein to investigate the immunological role of Z39Ig protein on various immune cells. The anti-Z39Ig mAb that we generated specifically bound to Z39Ig protein on human promonocytic THP-1 cells, monocytes isolated from human peripheral blood mononuclear cells (PBMC) and mature CD14(+) dendritic cells (DC) differentiated from umbilical-cord blood CD34(+) hematopoietic progenitor cells. In addition, a signal through the Z39Ig protein induced an obvious cell surface expression of HLA-DR on THP-1 cells mediated by MHC class II transactivator (CIITA). These data suggest that the Z39Ig protein might be a critical molecule to regulate an immune response mediated by phagocytosis and/or antigen presentation.
Kim
Jin-Kyung
JK
Immunomodulation Research Center, University of Ulsan, South Korea.
Choi
Eun Mi
EM
Shin
Hyo-Il
HI
Kim
Chang H
CH
Hwang
Sun-Hee
SH
Kim
Seong-Mi
SM
Kwon
Byoung S
BS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D018952:Antigens, CD34 / Q000276:immunology; D018076:DNA, Complementary / Q000096:biosynthesis; D005260:Female; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis; D019169:Jurkat Cells; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D009687:Nuclear Proteins / Q000096:biosynthesis; D011951:Receptors, Complement; D015534:Trans-Activators / Q000096:biosynthesis; D014162:Transfection
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D018076:DNA, Complementary; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D011951:Receptors, Complement; D015534:Trans-Activators; C414427:VSIG4 protein, human; D007371:Interferon-gamma