[Study on the presence of hepatitis B virus in first-trimester villi in pregnant women with hepatitis B surface antigen positive].
OBJECTIVE
To observe the presence of hepatitis B virus (HBV) in first-trimester villi cells from pregnant women carrying HBsAg.


METHODS
Immunohistochemical streptavidin-biotin peroxidase complex (SABC) staining with monoclonal HBsAg, hepatitis B core antigen (HBcAg) and PCR, in situ hybridization were used for detection of HBV infection markers in villi. Positive villi ultramicrostructures were observed with transmission electron microscope.


RESULTS
HBV was detected in 8 of 25 villi of HBsAg positive pregnant women, the positive rate was 32%. HBsAg was located in the decidual cell, trophoblastic cell and villous mesenchymal cell. HBV analog was detected in rough endoplasmic reticulum of trophoblastic cell.


CONCLUSIONS
HBV may infect villous cells in first-trimester pregnancy. It would be impossible for HBV to transmit the desmosomes.
Chang
Wen-hui
WH
Department of Epidemiology, Fourth Military Medical University, Xi'an 710032, China.
Xu
De-zhong
DZ
Yan
Yong-ping
YP
Du
Ke-jun
KJ
Men
Ke
K
Zhang
Jing-xia
JX
Wang
Jing-jun
JJ
Xu
Jian-qiu
JQ
Zhang
En-di
ED
Liu
Chen
C
Sun
Feng-mei
FM
D002824:Chorionic Villi / Q000648:ultrastructure / Q000821:virology*; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000097:blood / Q000276:immunology / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011261:Pregnancy Trimester, First
D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens