Identification of an immunogenic EWS-FLI1-derived HLA-DR-restricted T helper cell epitope.
Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFN(gamma) video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.
Meyer-Wentrup
Friederike
F
Department of Pediatrics, Children's Hospital Medical Center, Munich University of Technology, München, Germany. friederike.meyer-wentrup@medizin.uni-halle.de
Richter
Günther
G
Burdach
Stefan
S
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D003713:Dendritic Cells / Q000276:immunology; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000032:analysis; D015514:Oncogene Proteins, Fusion / Q000276:immunology*; D019151:Peptide Library; D010455:Peptides / Q000276:immunology; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D012512:Sarcoma, Ewing; D037182:T-Cell Antigen Receptor Specificity; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology*
C106031:EWS-FLI fusion protein; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D015514:Oncogene Proteins, Fusion; D019151:Peptide Library; D010455:Peptides; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D014157:Transcription Factors; D007371:Interferon-gamma