Gene expression profiling reveals an inflammatory process in the anx/anx mutant mice.
Anorexia (anx) is a recessive mutation that causes lethal starvation in homozygous mice. Studies of anx/anx mice hypothalamus have shown abnormalities in the orexigenic (NPY/AGRP neurons) and the anorexigenic (POMC/CART neurons) pathways. By gene expression profiling using cDNA and oligonucleotide microarrays, we have shown that a surexpression of genes involved in inflammatory process occurred in anx mice hypothalamus. This inflammatory process could be the cause of the anorexia phenotype observed in these mice.
Lachuer
Joel
J
Experimental Neurobiology and Physiopathology Laboratory, Inserm U433, University Claude Bernard Lyon1, 8 rue Guillaume Paradin, 69372 Lyon cedex 08, France. lachuer@univ-lyon1.fr
Ouyang
Ling
L
Legras
Catherine
C
Del Rio
Jo
J
Barlow
Carrolee
C
D000818:Animals; D000855:Anorexia / Q000235:genetics* / Q000503:physiopathology*; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D007031:Hypothalamus / Q000378:metabolism; D007249:Inflammation / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008818:Mice, Neurologic Mutants; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D009478:Neuropeptide Y; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin