title: Low temperature of GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor DnaK.
abstract: Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30 degrees C. Here we show that the synthetic lethality of deltatigdeltadnaK52 cells is abrogated either by growth below 30 degrees C or by overproduction of GroEL/GroES. At 23 degrees C deltatigdeltadnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of deltatigdeltadnaK52 cells at 30 degrees C and suppressed protein aggregation including proteins >/= 60 kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.
authors: Ciphergen Biosystems GmbH, Hannah-Vogt-Str. 1, 37085 Göttingen, Germany.
authors: S
authors: S
authors: Vorderwülbecke
authors: G
authors: G
authors: Kramer
authors: F
authors: F
authors: Merz
authors: T A
authors: TA
authors: Kurz
authors: T
authors: T
authors: Rauch
authors: B
authors: B
authors: Zachmann-Brand
authors: B
authors: B
authors: Bukau
authors: E
authors: E
authors: Deuerling
mesh_terms: D018835:Chaperonin 10 / Q000096:biosynthesis / Q000502:physiology*; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000502:physiology*; D003080:Cold Temperature; D004926:Escherichia coli / Q000254:growth & development*; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D019696:Peptidylprolyl Isomerase