Morphofunctional characteristics of blood mononuclear cells during in vitro culturing under dynamic conditions.
The primary culture of human blood mononuclear cells was incubated on fibrous membranes under a constant directional flow of the nutrient medium modulating intraocular fluid flow. Incubation was performed for 24, 48, and 72 h. Intracellular enzyme activity of mononuclear cells increased under dynamic conditions. Differentiation of blood monocytes and circulating fibroblast precursors into macrophageal cells and mature cells, respectively, was accelerated.
Krivosheina
O I
OI
Siberian State Medical University, Tomsk. olena2@yandex.ru
Zapuskalov
I V
IV
Khlusov
I A
IA
D000469:Alkaline Phosphatase / Q000032:analysis; D001773:Blood Cells; D043182:Carboxylesterase / Q000037:antagonists & inhibitors; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008264:Macrophages / Q000166:cytology; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D009283:Naphthol AS D Esterase; D012969:Sodium Fluoride / Q000494:pharmacology; D013997:Time Factors
D004791:Enzyme Inhibitors; D012969:Sodium Fluoride; D009283:Naphthol AS D Esterase; D043182:Carboxylesterase; D000469:Alkaline Phosphatase