title: Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG)( n ) telomeric probe in some species of Lepidoptera.
abstract: We have developed a simple method to resolve the sex chromosome constitution in females of Lepidoptera by using a combination of genomic in situ hybridization (GISH) and fluorescence in situ hybridization with (TTAGG)( n ) telomeric probe (telomere-FISH). In pachytene configurations of sex chromosomes, GISH differentiated W heterochromatin and telomere-FISH detected the chromosome ends. With this method we showed that Antheraea yamamai has a standard system with a fully differentiated W-Z sex chromosome pair. In Orgyia antiqua, we confirmed the presence of neo-W and neo-Z chromosomes, which most probably originated by fusion of the ancestral W and Z with an autosome pair. In contrast to earlier data, Orgyia thyellina females displayed a neo-ZW(1)W(2) sex chromosome constitution. A neo-WZ(1)Z(2) trivalent was found in females of Samia cynthia subsp. indet., originating from a population in Nagano, Japan. Whereas another subspecies collected in Sapporo, Japan, and determined as S. cynthia walkeri, showed a neo-W/neo-Z bivalent similar to O. antiqua, and the subspecies S. cynthia ricini showed a Z univalent (a Z/ZZ system). The combination of GISH and telomere-FISH enabled us to acquire not only reliable information about sex chromosome constitution but also an insight into sex chromosome evolution in Lepidoptera.
authors: Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University N9, W9, Kita-ku, Sapporo 060-8589, Japan.
authors: Atsuo
authors: A
authors: Yoshido
authors: Frantisek
authors: F
authors: Marec
authors: Ken
authors: K
authors: Sahara
mesh_terms: D000818:Animals; D005075:Biological Evolution; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007814:Larva / Q000166:cytology / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics*; D009036:Moths / Q000235:genetics; D010802:Phylogeny; D012730:Sex Chromosomes / Q000235:genetics*; D013045:Species Specificity; D016615:Telomere / Q000737:chemistry* / Q000235:genetics