Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase.
Adereth
Yair
Y
Medical University of South Carolina, Charleston, SC, USA.
Champion
Kristen J
KJ
Hsu
Tien
T
Dammai
Vincent
V
D017122:Bacteriophage T4 / Q000201:enzymology*; D011088:DNA Ligases / Q000378:metabolism*; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases