Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
The Arabidopsis gene COI1 is required for jasmonic acid (JA)-induced growth inhibition, resistance to insect herbivory, and resistance to pathogens. In addition, COI1 is also required for transcription of several genes induced by wounding or by JA. Here, we use microarray gene transcription profiling of wild type and coi1 mutant plants to examine the extent of the requirement of COI1 for JA-induced and wound-induced gene transcription. We show that COI1 is required for expression of approximately 84% of 212 genes induced by JA, and for expression of approximately 44% of 153 genes induced by wounding. Surprisingly, COI1 was also required for repression of 53% of 104 genes whose expression was suppressed by JA, and for repression of approximately 46% of 83 genes whose expression was suppressed by wounding. These results indicate that COI1 plays a pivotal role in wound- and JA signalling.
Devoto
Alessandra
A
School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK.
Ellis
Christine
C
Magusin
Andreas
A
Chang
Hur-Song
HS
Chilcott
Charles
C
Zhu
Tong
T
Turner
John G
JG
D000085:Acetates / Q000494:pharmacology*; D000872:Anthocyanins / Q000096:biosynthesis; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D016000:Cluster Analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013314:Stress, Mechanical; D013729:Terpenes / Q000378:metabolism
D000085:Acetates; D000872:Anthocyanins; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013729:Terpenes; C072239:methyl jasmonate