Human fetal neural stem cells in rat brain: effects of preculturing and transplantation.
The fate of human fetal stem/progenitor cells transplanted into rat brain depends on conditions of preculturing (long or short) and state and site of transplantation. Human nestin-positive stem cells cultured according to the short protocol did not migrate into hypoxic and normal brain after transplantation, but actively migrated in damaged spinal cord. After transplantation of long-cultured cells into the brain mainly committed neuroblasts and solitary nestin-positive cells migrated from the site of transplantation into the brain.
Revishchin
A V
AV
Institute of Problems of Ecology and Evolution, Russian Academy of Sciences, Moscow. aleksandrova@vigg.ru
Aleksandrova
M A
MA
Podgornyi
O V
OV
Marei
M V
MV
Poltavtseva
R A
RA
Korochkin
L I
LI
Stepanov
G A
GA
Sukhikh
G T
GT
D000818:Animals; D001921:Brain / Q000166:cytology*; D018929:Cell Culture Techniques; D002465:Cell Movement; D004622:Embryo, Mammalian / Q000166:cytology / Q000294:innervation; D005333:Fetus; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000502:physiology*; D014183:Transplantation, Heterologous
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin