title: Fluorescence detection system for capillary separations utilizing a liquid core waveguide with an optical fibre-coupled compact spectrometer.
abstract: A fluorescence detection system for capillary liquid separation methods is described. The system is based on a silica capillary coated with a low refractive index fluoropolymer Teflon AF that serves both as a separation channel and as a liquid core waveguide (LCW). A fibre-coupled laser excites separated analytes in a detection point and arising fluorescence is collected at one end of the LCW capillary into the other optical fibre which brings it to a compact charge-coupled device (CCD) array spectrometer installed in a desktop computer. No additional components such as focusing optics or filters are necessary. This system was used for detecting isoelectrically focused fluorescent low-molecular-mass pI (isoelectric point) markers and fluorescein isothiocyanate (FITC) labelled proteins. The ability of the system to acquire fluorescent spectra is also demonstrated.
authors: Department of Analytical Chemistry, Faculty of Science, Palacky University, tr. Svobody 26, 771 46 Olomouc, Czech Republic. kostal@iach.cz
authors: Vratislav
authors: V
authors: Kostal
authors: Marta
authors: M
authors: Zeisbergerova
authors: Karel
authors: K
authors: Slais
authors: Vladislav
authors: V
authors: Kahle
mesh_terms: D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005336:Fiber Optic Technology; D016650:Fluorescein-5-isothiocyanate; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D008904:Miniaturization; D011138:Polytetrafluoroethylene; D011506:Proteins / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011506:Proteins; D011138:Polytetrafluoroethylene; D016650:Fluorescein-5-isothiocyanate