The yeast osmosensitive mutant fps1Delta transformed by the cauliflower BobTIP1;1 aquaporin withstand a hypo-osmotic shock.
Osmoregulation plays an important role in cellular responses to osmotic stress in plants and in yeast. Aquaporins contribute to osmotic adjustment by facilitating transport of water or solutes across membranes. The tonoplastic water channel BobTIP1;1 (original name BobTIP26-1) genes are upregulated during dessication stress in cauliflower meristematic tissue. To investigate the physiological importance of BobTIP1;1, we expressed it in a Saccharomyces cerevisiae osmosensitive mutant fps1Delta. We showed that the defect in the yeast glycerol plasma membrane transporter is complemented by a plant cDNA encoding the aquaporin BobTIP1;1 which is localized in the vacuolar membrane of the complemented yeast cells. To our knowledge, this is the first example of a plant aquaporin for which localization in the vacuolar membrane of yeast cells is related to an osmoresistant phenotype under hypo-osmotic shock.
Prudent
Sébastien
S
UMR/INRA 1088/CNRS 5184/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, Université de Bourgogne, F-21065 Dijon Cedex, France.
Marty
Francis
F
Charbonnier
Maryse
M
D020346:Aquaporins / Q000737:chemistry*; D015153:Blotting, Western; D001937:Brassica; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics*; D009154:Mutation; D009995:Osmosis; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013997:Time Factors; D014617:Vacuoles / Q000378:metabolism; D014867:Water / Q000737:chemistry
D020346:Aquaporins; C111630:BobTIP26-1 protein, Brassica oleracea; D018076:DNA, Complementary; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014867:Water; D005990:Glycerol