title: [A preliminary proteomic analysis of tauopathies].
abstract: OBJECTIVE
  To investigate the molecular mechanisms of tauopathies. Comparative proteomic analysis of brain proteins was employed to study 4 patients with tauopathies as compared with 4 controls.
  
  
  METHODS
  The brains of subjects who died without clinical or pathological involvement of nervous system and brains of patients with tauopathies were obtained at autopsy. The brain proteins were run by immobilized pH gradient (IPG) isoelectric focusing electrophoresis as the first dimension, and then run by vertical SDS-PAGE as the second dimension. The maps were visualized by silver staining or colloidal coomassie blue and analyzed with Image Master 2D Elite software. The proteins of interest were in-gel digested and identified using MALDI-TOF mass spectrometry or MALDI-TOF/TOF tandem mass spectrometry.
  
  
  RESULTS
  18 protein spots were differentially expressed as compared with age-matched nondemented control brains which were identified as glyceraldehyde 3-phosphate dehydrogenase, uracil DNA glycosylase, human superoxide dismutase, isocitrate dehydrogenase subunit, synaptotagmin I, thioredoxin peroxidase 1, glial fibrillary acidic protein, p25 alpha, enoyl coenzyme A hydratase short chain 1, pyridoxine-5'-phosphate oxidase, Mn-superoxide dismutase and alpha enolase, antioxidant protein 2, ferritin heavy chain, glutamate dehydrogenase precursor, peptidyl-prolyl cis-trans isomerase A, serum albumin precursor and dihydropyrimidinase-related protein 2.
  
  
  CONCLUSIONS
  We got a number of related-proteins of tauopathies. Some proteins are quite useful for discovering the molecular mechanisms of tauopathies and may be helpful for diagnosis and of treatment tauopathies.
authors: Department of Geriatric Neurology, Chinese PLA General Hospital, Beijing 100853, China.
authors: Guo-feng
authors: GF
authors: Yang
authors: Lu-ning
authors: LN
authors: Wang
authors: Jian-guo
authors: JG
authors: Ji
authors: Si-zhi
authors: SZ
authors: He
authors: Ming-wei
authors: MW
authors: Zhu
authors: Qing-song
authors: QS
authors: Wang
mesh_terms: D000368:Aged; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D024801:Tauopathies / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins