Analysis of gene expression during staurosporine-induced neuronal differentiation of human prostate cancer cells.
Staurosporine induces neuronal differentiation and suppresses malignancy of human prostate cancer TSU-Pr1 cells. To investigate the mechanism underlying neuronal differentiation and suppression of malignancy, we used cDNA microarrays to examine gene expression profiles in TSU-Pr1 cells treated with staurosporine. mRNAs isolated from untreated and staurosporine-treated TSU-Pr1 cells were hybridised to microarrays consisting of approximately 9100 genes. Changes in gene expression were verified by Northern blot analysis. Staurosporine-responsive genes were involved in a variety of cellular functions including growth regulation, differentiation, replication, DNA repair, G2/M transition and inhibition of apoptosis. Interestingly, expression of genes associated with cell proliferation and malignancy, such as Cyr61 and CTGF, was reduced. Expression of CD73/NT5E, which is involved in neuronal differentiation, was increased. In the present study, we identified various staurosporine-responsive genes in TSU-Pr1 cells. Further studies of the roles of these genes may clarify the mechanisms underlying neuronal differentiation and inhibition of malignancy by staurosporine and identify better approaches for the prevention and treatment of prostate cancer.
Shimizu
Takahisa
T
Department of Hygiene-Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki Noda-shi, Chiba 278-8510, Japan. takahisa@rs.noda.tus.ac.jp
Okayama
Akiko
A
Inoue
Toshihiro
T
Takeda
Ken
K
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology*
D012333:RNA, Messenger; D019311:Staurosporine