Synthesis and biological activity of M6-P and M6-P analogs on fibroblast and keratinocyte proliferation.
A new synthetic route to obtain the carboxylate analog of mannose 6-phosphate (M6-P) is presented. The effects of the M6-P, the carboxylate and two other analogs (the phosphonate and the alpha,beta ethylenic carboxylate) on the proliferation of human keratinocytes and dermal fibroblasts as well as on the proliferation of a murine fibroblast cell line, 3T3-J2 are tested. We observed that M6-P is a potent inhibitor of proliferation of both fibroblasts and keratinocytes. Among its analogs, the phosphonate showed a similar effect on human dermal fibroblasts but not on keratinocytes.
Clavel
Caroline
C
Laboratoire de Chimie Biomoléculaire, FRE 5032 (UM2, CNRS), ERT 5-ENSCM, Montpellier cedex 5, France.
Barragan-Montero
Véronique
V
Garric
Xavier
X
Molès
Jean-Pierre
JP
Montero
Jean-Louis
JL
D016475:3T3 Cells; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008360:Mannosephosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice
D008360:Mannosephosphates; C027693:mannose-6-phosphate