title: The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication.
abstract: The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcDeltaie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcDeltaie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAcDeltaie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644, 2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells.
authors: Entomology Department, The Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel.
authors: Liqun
authors: L
authors: Lu
authors: Lu
authors: L
authors: Liqun
authors: Hadassah
authors: H
authors: Rivkin
authors: Nor
authors: N
authors: Chejanovsky
mesh_terms: D000818:Animals; D002460:Cell Line; D017874:Immediate-Early Proteins / Q000502:physiology*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D013045:Species Specificity; D015534:Trans-Activators / Q000502:physiology*; D014779:Virus Replication
chemical_list: D017874:Immediate-Early Proteins; D015534:Trans-Activators; C450080:ie0 protein, baculovirus