[Preparation of 5-fluorouracil-peanut agglutinin conjugate and determination of the ratio of drug to conjugate].
5-fluorouracil was combined with peanut agglutinin by a water-soluble carbodiimide to prepare the tumor target conjugate of 5-fluorouracil-peanut agglutinin and the ratio of drug to conjugate was determined by the modified trinitrobenzenesulfonic acid method (TNBS). The ratio of drug to conjugate was 76.33%. The result showed that 5-fluorouracil could link to the peanut agglutinin by EDC/NHS crosslinking with high drug ratio.
Cai
Qin
Q
West China Pharmacy College of Sichuan University, Chengdu 610041, China.
Meng
Yanfa
Y
Zhang
Zhirong
Z
D000970:Antineoplastic Agents / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D005472:Fluorouracil / Q000737:chemistry*; D019887:Peanut Agglutinin / Q000737:chemistry*
D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D019887:Peanut Agglutinin; D005472:Fluorouracil