title: Evaluation of radiation-induced oral mucositis by optical coherence tomography.
abstract: OBJECTIVE
  Optical coherence tomography (OCT) imaging was evaluated to determine if radiation-induced mucosal damage could be noninvasively monitored in real time and correlated with histopathologic findings.
  
  
  METHODS
  Female C3H mice, ages 7 to 9 weeks, four per group, were immobilized in a custom-made Lucite jig and received 0, 15, 22.5, and 25 Gy in a single fraction to their oral cavity. OCT images were acquired of proximal, middle, and distal aspects of the dorsum of the tongue on days 0, 1, 3, 5, and 7 post-irradiation. Animals were sacrificed on day 7 and samples taken for histologic evaluation. OCT images were visually examined and also quantified by image analysis and compared with histologic findings.
  
  
  RESULTS
  Tongues removed 7 days post-irradiation showed no visible damage; however, upon staining with toluidine blue, ulcers at the base of the tongue became visible (100% for 25 Gy, 75% after 22.5 Gy, and 0% after 15 Gy). Visual inspection of OCT images qualitatively compared with histologic findings and quantitative image analysis of the OCT images (effective light penetration depth) revealed significant changes 7 days post-irradiation compared with unirradiated controls for the base of the tongue.
  
  
  CONCLUSIONS
  OCT allows for direct noninvasive real-time acquisition of digitally archivable images of oral mucosa and can detect radiation-induced changes in the mucosa before visual manifestation. OCT may be a useful technique to quantify subclinical radiation-induced mucosal injury in experimental chemoradiation clinical trials.
authors: Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA.
authors: Thierry M
authors: TM
authors: Muanza
authors: Ana P
authors: AP
authors: Cotrim
authors: Mathew
authors: M
authors: McAuliffe
authors: Anastasia L
authors: AL
authors: Sowers
authors: Bruce J
authors: BJ
authors: Baum
authors: John A
authors: JA
authors: Cook
authors: Felix
authors: F
authors: Feldchtein
authors: Paul
authors: P
authors: Amazeen
authors: C Norman
authors: CN
authors: Coleman
authors: James B
authors: JB
authors: Mitchell
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D009061:Mouth Mucosa; D011832:Radiation Injuries / Q000175:diagnosis* / Q000662:veterinary; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013280:Stomatitis / Q000175:diagnosis* / Q000209:etiology* / Q000662:veterinary; D041623:Tomography, Optical Coherence