title: Improved detection of allergen-specific T-cell responses in allergic contact dermatitis through the addition of 'cytokine cocktails'.
abstract: The gold standard for the diagnosis of allergic hypersensitivity is skin patch testing with the suspected allergens. This diagnostic tool, however, has distinct disadvantages, and therefore the development of alternative or complementary in vitro tests is of great importance. In this study, we evaluate the applicability of an in vitro test method, as developed earlier for nickel allergy, to detect allergen-specific T cells in the blood of patients allergic to frequent sensitizers (chromate, cobalt, paraphenylenediamine, fragrances and chloromethyl-isothiazolinone). Peripheral blood mononuclear cells (PBMCs) of allergic patients and healthy controls were cultured in the absence or presence of allergen. Additionally, type 1 (IL-7 and IL-12) or type 2 (IL-7 and IL-4) stimulating cytokines were added; after 6-day proliferation, IFN-gamma and IL-5 secretions were determined. Without the addition of cytokines, consistent allergen-induced proliferation was observed in PBMCs of nickel-allergic patients only. By contrast, the addition of type 1 or type 2 stimulating cytokines resulted in a significantly enhanced allergen-specific proliferation for all allergens tested (sensitivity increased from 26 to 43% or 38%, respectively, P < 0.05). In these cultures, allergen-induced IFN-gamma and IL-5 secretion was also significantly increased, compared to healthy controls (P < 0.05, for IFN-gamma sensitivity 79%, specificity 93%; for IL-5 sensitivity 74%, specificity 81%). In conclusion, these results demonstrate an increased proliferative capacity and cytokine production by allergen-specific T cells from allergic patients, but not of healthy individuals upon stimulation with allergens in combination with type 1 or 2 skewing cytokines. The present data warrant further exploration of the application of this test to a broader set of allergens.
authors: Department of Dermatology, VU University Medical Center, Amsterdam, The Netherlands.
authors: Helen
authors: H
authors: Moed
authors: Mary
authors: M
authors: von Blomberg
authors: Derk P
authors: DP
authors: Bruynzeel
authors: Rik
authors: R
authors: Scheper
authors: Susan
authors: S
authors: Gibbs
authors: Thomas
authors: T
authors: Rustemeyer
mesh_terms: D000328:Adult; D000485:Allergens / Q000378:metabolism*; D049109:Cell Proliferation; D004396:Coloring Agents / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015851:Interleukin-7 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009532:Nickel / Q000494:pharmacology; D010328:Patch Tests / Q000379:methods*; D010655:Phenylenediamines / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
chemical_list: D000485:Allergens; D004396:Coloring Agents; D016207:Cytokines; D015850:Interleukin-6; D015851:Interleukin-7; D010655:Phenylenediamines; D018664:Interleukin-12; D015847:Interleukin-4; D009532:Nickel; D007371:Interferon-gamma; C029728:4-phenylenediamine