title: Flow-through microdispenser for interfacing micro-HPLC to Raman and mid-IR spectroscopic detection.
abstract: A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.
authors: Institute for Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9-164, A-1060 Vienna, Austria.
authors: Izabella
authors: I
authors: Surowiec
authors: Josefa R
authors: JR
authors: Baena
authors: Johannes
authors: J
authors: Frank
authors: Thomas
authors: T
authors: Laurell
authors: Johan
authors: J
authors: Nilsson
authors: Marek
authors: M
authors: Trojanowicz
authors: Bernhard
authors: B
authors: Lendl
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005419:Flavonoids / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis*; D008832:Microchemistry / Q000295:instrumentation*; D009862:Online Systems; D010636:Phenols / Q000032:analysis; D059808:Polyphenols; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014920:Wine / Q000032:analysis
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D059808:Polyphenols