title: Secretion of monocyte chemotactic protein-1 by human uterine epithelium directs monocyte migration in culture.
abstract: OBJECTIVE
  To determine whether primary human uterine epithelial cells in culture are able to influence monocyte chemotaxis and to establish whether the causal agent of chemotaxis is monocyte chemotactic protein (MCP)-1.
  
  
  METHODS
  Tissue culture study.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  Women aged 23 to 53 years who were undergoing hysterectomy (n=7).
  
  
  METHODS
  Primary human endometrial epithelial cells were acquired from surgical specimens and grown to confluence and high transepithelial resistance. Conditioned media from epithelial cultures were analyzed for the presence of MCP-1 and for capacity to affect monocyte chemotaxis using the THP-1 monocyte line. Antibody neutralization of conditioned media was used to establish the role of MCP-1 in chemotaxis.
  
  
  METHODS
  Assay of conditioned media for MCP-1, quantitative measurement of monocyte chemotaxis to conditioned media, and inhibition of chemotaxis by antibody neutralization of MCP-1.
  
  
  RESULTS
  Primary endometrial epithelial cells in monolayer culture secrete MCP-1 to both the apical and basolateral compartments. Monocyte chemotactic protein-1 was identified as the primary agent of monocyte chemotaxis by antibody neutralization.
  
  
  CONCLUSIONS
  These findings suggest that biologically active MCP-1 is secreted into both the uterine lumen as well as the underlying stroma and that it mediates the presence of monocytes, macrophages, and other immune cells in the uterine endometrium.
authors: Department of Obstetrics and Gynecology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA. richard.a.meter@hitchcock.org
authors: Richard A
authors: RA
authors: Meter
authors: Charles R
authors: CR
authors: Wira
authors: John V
authors: JV
authors: Fahey
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism* / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D004847:Epithelial Cells / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2