Trypanosoma vivax: a novel method for purification from experimentally infected sheep blood.
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
González
L E
LE
Universidad Simón Bolívar, Departamento de Biología Celular, Grupo deBioquímica e Inmunología de Hemoparásitos, Caracas, Venezuela.
García
J A
JA
Núñez
C
C
Perrone
T M
TM
González-Baradat
B
B
Gonzatti
M I
MI
Reyna-Bello
A
A
D000818:Animals; D002500:Centrifugation, Isopycnic / Q000662:veterinary; D002848:Chromatography, DEAE-Cellulose / Q000662:veterinary; D007165:Immunosuppression Therapy; D018512:Parasitemia / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D015800:Protozoan Proteins / Q000032:analysis; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology*; D016689:Trypanosoma vivax / Q000737:chemistry / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D015800:Protozoan Proteins