Isogenic autosomes to be applied in optimal screening for novel mutants with viable phenotypes in Drosophila melanogaster.
Most insertional mutagenesis screens of Drosophila performed to date have not used target chromosomes that have been checked for their suitability for phenotypic screens for viable phenotypes. To address this, we have generated a selection of stocks carrying either isogenized second chromosomes or isogenized third chromosomes, in a genetic background derived from a Canton-S wild-type strain. We have tested these stocks for a range of behavioral and other viable phenotypes. As expected, most lines are statistically indistinguishable from Canton-S in most phenotypes tested. The lines generated are now being used as target chromosomes in mutagenesis screens, and the characterization reported here will facilitate their use in screens of these lines for behavioral and other viable phenotypes.
Sharma
Punita
P
Department of Genetics, University of Cambridge, Cambridge, UK.
Asztalos
Zoltan
Z
Ayyub
Champakali
C
de Bruyne
Marien
M
Dornan
Anthony J
AJ
Gomez-Hernandez
Araceli
A
Keane
John
J
Killeen
James
J
Kramer
Susanne
S
Madhavan
Mayur
M
Roe
Helen
H
Sherkhane
Pradeep Dagadu
PD
Siddiqi
Khalid
K
Silva
Elizabeth
E
Carlson
John R
JR
Goodwin
Stephen F
SF
Heisenberg
Martin
M
Krishnan
Kits
K
Kyriacou
Charalambos P
CP
Partridge
Linda
L
Riesgo-Escovar
Juan
J
Rodrigues
Veronica
V
Tully
Tim
T
O'Kane
Cahir J
CJ
D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001547:Benzaldehydes / Q000494:pharmacology; D002940:Circadian Rhythm / Q000235:genetics; D003307:Copulation; D004251:DNA Transposable Elements / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005820:Genetic Testing / Q000379:methods; D018404:Isochromosomes / Q000235:genetics*; D007858:Learning / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D008297:Male; D009154:Mutation; D010243:Paralysis / Q000235:genetics; D010641:Phenotype; D012726:Sexual Behavior, Animal; D014921:Wings, Animal / Q000033:anatomy & histology
D000777:Anesthetics; D001547:Benzaldehydes; D004251:DNA Transposable Elements; C032175:benzaldehyde