An alternative pretreatment procedure in animal transmissible spongiform encephalopathies diagnosis using PrPsc immunohistochemistry.
Because of its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used more often in the diagnosis of transmissible spongiform encephalopathies (TSEs), such as scrapie and bovine spongiform encephalopathy (BSE). PrPsc IHC requires a combination of pretreatments (chemical, heating, and enzymatic). The method of application may depend on the anti-prion antibody considered. If these pretreatments are efficient for diagnostic purpose, it may, however, be interesting to use an alternative method to efficiently detect PrPsc IHC immunohistochemically using chemical pretreatments solely. Here we describe such pretreatments reporting the difficulty (section adhesion) but also the potential advantages of such methods (easy, quick, inexpensive, and amplifying effect).
Bencsik
Anna A
AA
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité Agent Transmissible Non Conventionnel (ATNC), Lyon, France. a.bencsik@lyon.afssa.fr
Debeer
Sabine O S
SO
Baron
Thierry G M
TG
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D018622:PrP 27-30 Protein / Q000276:immunology / Q000378:metabolism; D018620:PrPSc Proteins / Q000032:analysis*; D012608:Scrapie / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D012756:Sheep
D000906:Antibodies; D018620:PrPSc Proteins; D018622:PrP 27-30 Protein