title: In vivo and in vitro proliferative and differentiation activity of human embryonic retinal cells.
abstract: Differentiation of human embryonic retinal cells (20-22 weeks gestation) was studied using morphological, immunohistochemical, and biomolecular approaches. The retina included several regions differing by the degree of cell differentiation. Mitoses were rarely found in the marginal zone. This zone contained low differentiated cells. The central retinal area consisted of typical layers with differentiated cells. Culturing was accompanied by the formation of aggregates and neurospheres, where mitoses and progenitor or differentiated cells expressing markers of photoreceptors, neurons, and glia were found.
authors: N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Russia. pinag@mail.ru
authors: I G
authors: IG
authors: Panova
authors: O V
authors: OV
authors: Podgornyi
authors: B
authors: B
authors: Verdiev
authors: Yu A
authors: YA
authors: Smirnova
authors: R A
authors: RA
authors: Poltavtseva
authors: E N
authors: EN
authors: Grigoryan
authors: R D
authors: RD
authors: Zinov'eva
authors: M A
authors: MA
authors: Aleksandrova
authors: G T
authors: GT
authors: Sukhikh
authors: V I
authors: VI
authors: Mitashov
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051603:Recoverin / Q000235:genetics; D012160:Retina / Q000166:cytology* / Q000196:embryology*; D014404:Tubulin / Q000235:genetics; D014746:Vimentin / Q000235:genetics
chemical_list: D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D014404:Tubulin; D014746:Vimentin; D051603:Recoverin