title: Delivery of antisense oligonucleotides using cholesterol-modified sense dendrimers and cationic lipids.
abstract: Cholesterol modified mono-, di-, and tetrameric oligonucleotides were synthesized and hybridized with antisense oligonucleotides to study their incorporation in cationic liposomes together with the influence of this dendrimeric delivery system on biological activity. Electrostatic interactions seem to play the most important role during complexation with cationic lipids. This oligonucleotide formulation gives a small but significant increase in the inhibition of P-glycoprotein expression in a cellular system.
authors: Laboratory for Medicinal Chemistry, Rega Institute, Catholic University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors: Patrick
authors: P
authors: Chaltin
authors: Anca
authors: A
authors: Margineanu
authors: Damien
authors: D
authors: Marchand
authors: Arthur
authors: A
authors: Van Aerschot
authors: Jef
authors: J
authors: Rozenski
authors: Frans
authors: F
authors: De Schryver
authors: Andreas
authors: A
authors: Herrmann
authors: Klaus
authors: K
authors: Müllen
authors: Rudolph
authors: R
authors: Juliano
authors: Michael H
authors: MH
authors: Fisher
authors: Hyunmin
authors: H
authors: Kang
authors: Steven
authors: S
authors: De Feyter
authors: Piet
authors: P
authors: Herdewijn
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002412:Cations; D002784:Cholesterol / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D002412:Cations; D008055:Lipids; D016376:Oligonucleotides, Antisense; D002784:Cholesterol