Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands.
A psychrotolerant, Gram-negative, motile bacterium, designated CK 47(T), was isolated from sea water off the subantarctic Kerguelen islands (50 degrees 40' S 68 degrees 25' E). The isolate grew optimally at 22 degrees C and minimum and maximum temperature of growth were 4 and 37 degrees C, respectively. It required Na(+) for growth and exhibited optimum growth at pH 8.5 and 4% NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47(T) had Q9 as the major respiratory quinone and C(16 : 0) (21.7%), C(17:0) (21.3%), C(18:0) (5.7%), C(18:1)omega7c (9.0%) and C(18:1)omega9c (31.4%) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47(T) formed a coherent cluster within the genus Marinobacter. It exhibited highest 16S rRNA gene sequence similarity of 96.8% with Marinobacter lipolyticus. However, the level of DNA-DNA relatedness between strain CK47(T) and M. lipolyticus was only 55%. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47(T) is considered to represent a novel species of the genus Marinobacter. The name Marinobacter maritimus sp. nov. is proposed, with CK 47(T) (=JCM 12521(T)=MTCC 6519(T)) as the type strain.
Shivaji
S
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Gupta
Pratima
P
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Chaturvedi
Preeti
P
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Suresh
K
K
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Delille
Daniel
D
Université P. & M. Curie (Paris 6), Observatoire Océanologique de Banyuls, CNRS URA 2071, F-66650 Banyuls sur Mer, France.
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S