title: Degradation of myosin light chain in isolated rat hearts subjected to ischemia-reperfusion injury: a new intracellular target for matrix metalloproteinase-2.
abstract: BACKGROUND
  Matrix metalloproteinase-2 (MMP-2) contributes to cardiac dysfunction resulting from ischemia-reperfusion (I/R) injury. MMP-2 not only remodels the extracellular matrix but also acts intracellularly in I/R by degrading troponin I. Whether other intracellular targets exist for MMP-2 during I/R is unknown.
  
  
  RESULTS
  Isolated rat hearts were subjected to 20 minutes of ischemia and 30 minutes of reperfusion. The impaired recovery of mechanical function of the heart was attenuated by the MMP inhibitors o-phenanthroline or doxycycline. Quantitative 2D electrophoresis of homogenates of aerobically perfused hearts (control) or those subjected to I/R injury (in the presence or absence of MMP inhibitors) showed 3 low-molecular-weight proteins with levels that were significantly increased upon I/R injury and normalized to control levels by MMP inhibitors. Mass spectrometry analysis identified all 3 proteins as fragments of myosin light chain 1, which possesses theoretical cleavage recognition sequences for MMP-2 and is rapidly degraded by it in vitro. The association of MMP-2 with the thick myofilament in fractions prepared from I/R hearts was observed with immunogold electron microscopy, gelatin zymography for MMP-2 activity, and immunoprecipitation. MMP-2 was found to cleave myosin light chain 1 between tyrosine 189 and glutamine 190 at the C terminus.
  
  
  CONCLUSIONS
  Our results demonstrate that myosin light chain 1 is another novel substrate for MMP-2 in the cardiomyocyte and that its degradation may contribute to contractile dysfunction resulting from I/R injury to the heart.
authors: Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada.
authors: Grzegorz
authors: G
authors: Sawicki
authors: Hernando
authors: H
authors: Leon
authors: Jolanta
authors: J
authors: Sawicka
authors: Meltem
authors: M
authors: Sariahmetoglu
authors: Costas J
authors: CJ
authors: Schulze
authors: Paul G
authors: PG
authors: Scott
authors: Danuta
authors: D
authors: Szczesna-Cordary
authors: Richard
authors: R
authors: Schulz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000502:physiology*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D018994:Myosin Light Chains / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D051381:Rats; D017207:Rats, Sprague-Dawley; D012518:Sarcomeres / Q000201:enzymology
chemical_list: D018994:Myosin Light Chains; C076659:myosin light chain I; D020778:Matrix Metalloproteinase 2