Analysis of anatoxin-a using polyaniline as a sorbent in solid-phase microextraction coupled to gas chromatography-mass spectrometry.
A simple and sensitive method for determining anatoxin-a in aqueous samples was developed using solid-phase microextraction (SPME) and gas chromatography with mass spectrometry (GC-MS) detection. Three forms of polyaniline (PANI) films and a single form of polypyrrole (PPY) film were prepared and applied for SPME. The extraction properties of these films to anatoxin-a were examined and it was shown that leucoemeraldine form of PANI displayed a better selectivity to this compound. SPME conditions were optimized by selecting the appropriate extraction parameters, including type of coating (leucoemeraldine form of PANI at 32 microm thicknesses), salt concentration (10%, w/v), time of extraction (30 min) and stirring rate (1000 rpm). The calibration curve was linear in the range from 50 to 10,000 ng/ml, with the detection limit (S/N = 3) of 11.2 ng/ml. This method was successfully applied for the analysis of anatoxin-a in the cultured media of two species of cyanobacteria.
Ghassempour
Alireza
A
Department of Phytochemistry, Medicin Plant Institute, Shahid Beheshti University, P.O. Box 19835-389, Tehran, Iran. a-ghasempour@sbu.ac.ir
Najafi
Nahid Mashkouri
NM
Mehdinia
Ali
A
Davarani
Saied Saeed Hosseiny
SS
Fallahi
Maryam
M
Nakhshab
Maryam
M
D000814:Aniline Compounds / Q000737:chemistry*; D001427:Bacterial Toxins / Q000032:analysis*; D000087522:Cyanobacteria Toxins; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis*; D008832:Microchemistry; D052998:Microcystins; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014326:Tropanes
D000814:Aniline Compounds; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D011108:Polymers; D011758:Pyrroles; D014326:Tropanes; C416807:polyaniline; C067635:polypyrrole; C509783:anatoxin a