The Cre-loxP recombination-based reporter system for plant transcriptional expression studies.
To facilitate the characterization of plant genes, the Cre-loxP site-specific recombination system was adapted to make reporter vectors for plant expression studies. This system allows promoter fragments to be cloned into a small vector (univector) and subsequently recombined in vitro with binary vectors containing different reporter genes precisely at near-perfect efficiency. We have constructed univector-adapted vectors with three reporters, beta-glucuronidase, luciferase, and green fluorescent protein, and a BASTA-resistance gene for selection of plant transformants. Expression in plants using the new system was validated by comparison to conventional reporter vectors. These new vectors are efficient and economical alternatives to the other plant reporter vectors currently available. The royalty-free Cre-loxP system serves as a platform for the future expansion of recombination-based cloning vectors for plant research.
Shigaki
Toshiro
T
Children's Nutrition Research Center, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA.
Vyzasatya
Ravindranadha R
RR
Sivitz
Ali B
AB
Ward
John M
JM
Sze
Heven
H
Hirschi
Kendal D
KD
D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D005638:Fruit / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D017923:Sodium-Hydrogen Exchangers; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases; D005982:Glutathione Transferase; C045073:Cre recombinase; D019426:Integrases; D005966:Glucuronidase