Quantitative sandwich ELISA for the determination of lupine (Lupinus spp.) in foods.
The use of lupine in foods has increased considerably during the past decade, reflected by a corresponding increase in reported lupine-induced allergic incidents. Lupine allergy may arise either by primary sensitization or by clinical cross-reactivity in peanut-allergic persons. Detection of lupine proteins in food has previously been based on the use of patient serum. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of lupine in processed foods was developed, using a polyclonal rabbit antilupine capture antibody and a biotinylated conjugate of the same antibody for detection. The antibody was highly specific for lupine, apart from minor cross-reactivities to other legumes. The assay had a detection limit of 1 mug/g and was successfully used to quantify lupine protein in various food matrixes. Recoveries ranged from 60 to 116%, while the intra-and interassay coefficients of variation were <6% and <21%, respectively.
Holden
Lise
L
National Veterinary Institute, Post Office Box 8156 Dep., N-0033 Oslo, Norway. lise.holden@vetinst.no
Faeste
Christiane K
CK
Egaas
Eliann
E
D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity; D031297:Lupinus / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D010940:Plant Proteins