Determination of tetracyclines residues in honey using high-performance liquid chromatography with potassium permanganate-sodium sulfite-beta-cyclodextrin chemiluminescence detection.
A novel method was developed for the simultaneous determination of tetracycline antibiotic (TCA) residues such as oxytetracycline (OTC), tetracycline (TC), and metacycline (MTC) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the chemiluminescent enhancement by TCAs of the potassium permanganate-sodium sulfite-beta-cyclodextrin system in a phosphoric acid medium. The separation was carried out with an isocratic elution using a mixture of acetonitrile and 0.001 M phosphoric acid. For the three TCAs, the detection limits at a signal-to-noise of 3 ranged from 0.9 to 5.0 ng/ml. The relative standard deviations for the determination of TCAs ranged from 3.1 to 7.4% within a day (n=11) and ranged from 2.2 to 8.6% in 3 days (n=9), respectively. The method was successfully applied to the determination of TCA residues in honey samples. The possible mechanism of the CL reaction was also discussed.
Wan
Guo-Hui
GH
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
Cui
Hua
H
Zheng
Hai-Song
HS
Zhou
Jian
J
Liu
Li-Juan
LJ
Yu
Xiao-Feng
XF
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006722:Honey / Q000032:analysis*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008690:Methacycline / Q000032:analysis / Q000737:chemistry; D010118:Oxytetracycline / Q000032:analysis / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry; D015203:Reproducibility of Results; D013447:Sulfites / Q000737:chemistry; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
D013447:Sulfites; D013754:Tetracyclines; D047392:beta-Cyclodextrins; D011196:Potassium Permanganate; D008690:Methacycline; C031215:betadex; C025026:sodium sulfite; D010118:Oxytetracycline