Effects of yeast stress and pH on 3-monochloropropanediol (3-MCPD)-producing reactions in model dough systems.
A major precursor of 3-monochloropropanediol (3-MCPD) in leavened cereal products is glycerol, which is formed as a natural by-product of yeast fermentation. However, yeast metabolism is affected by stresses such as low osmotic pressure from, for example, the incorporation of sugar or salt in the dough recipe. Tests with model doughs have shown that glycerol production was proportional to yeast mass and limited by available sugars, but that high levels of yeast inhibited 3-MCPD formation. The yeast fraction responsible for the inhibition of 3-MCPD in model dough was shown to be the soluble cytosol proteins, and the inhibition mechanism could be explained by the known reactions of 3-MCPD and/or its precursors with ammonia/amino acids (from yeast proteins). Added glucose did not increase the production of glycerol by yeast but it did promote the generation of 3-MCPD in cooked doughs. The latter effect was attributed to the removal of 3-MCPD inhibitors such as ammonia and amino acids by their reactions with added glucose (e.g. Maillard). The thermal generation of organic acids from added glucose also reduced the pH of cooked doughs, so the effect of pH and short-chain organic acids on 3-MCPD generation in dough was measured. There was a good correlation between initial dough pH and the level of 3-MCPD generated. The effect was weaker than that predicted by simple kinetic modelling, suggesting that the involvement of H+ and/or the organic acid was catalytic. The results showed that modifications to dough recipes involving the addition of reducing sugars and/or organic acids can have a significant impact on 3-MPCD generation in bakery products.
Hamlet
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RHM Technology Ltd, The Lord Rank Centre, High Wycombe, UK. colin.g.hamlet@rhm.com
Sadd
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D000596:Amino Acids / Q000378:metabolism; D003296:Cooking; D005433:Flour; D005506:Food Contamination; D005511:Food Handling / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D015003:Yeasts / Q000378:metabolism*; D000517:alpha-Chlorohydrin
D000596:Amino Acids; D005656:Fungal Proteins; D000517:alpha-Chlorohydrin; D005947:Glucose; D005990:Glycerol