title: Trypanosoma cruzi modulates the expression of Rabs and alters the endocytosis in mouse cardiomyocytes in vitro.
abstract: Chagas disease is an incurable illness caused by the protozoan Trypanosoma cruzi. Cardiomyocytes represent important targets for the parasite infection and alterations in their physiology were reported. Because endocytosis is involved in different cellular events and guanosine triphosphatase (GTPase) Rab proteins play important roles in various aspects of the membrane traffic, our aim was to characterize the expression of Rab proteins in T. cruzi-infected cardiomyocytes, which displayed a downregulation of Rab7 and Rab11, whereas the expression of Rab5a was maintained in the infected cultures even after longer periods of parasite internalization, but early endosome antigen 1 was partially downregulated. The parasite infection also decreased the uptake of fluid phase ligands by the cardiac cultures. The regulation of GTPase proteins and effector molecules can contribute to the altered physiology of the host cells by modifying the normal incoming of nutrients as well as interfering with other important events related to the endocytic pathway.
authors: Departamento Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brasil.
authors: Denise G J
authors: DG
authors: Batista
authors: Cristiane F
authors: CF
authors: Silva
authors: Renata A
authors: RA
authors: Mota
authors: Lilian C
authors: LC
authors: Costa
authors: Maria N L
authors: MN
authors: Meirelles
authors: Marcelo
authors: M
authors: Meuser-Batista
authors: Maria N C
authors: MN
authors: Soeiro
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis; D002478:Cells, Cultured; D004705:Endocytosis; D005786:Gene Expression Regulation; D008024:Ligands; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000502:physiology*; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis*; D020696:rab5 GTP-Binding Proteins / Q000096:biosynthesis*; D000090802:rab7 GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
chemical_list: D001324:Autoantigens; D008024:Ligands; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins; D000090802:rab7 GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C093846:early endosome antigen 1; C000716465:rab7 GTP-binding proteins, mouse