[Effect of nm23-H1 on reversing malignant phenotype on human lung cancer cell line L9981].
nm23-H1 is a proven tumor metastasis suppressive gene, tumor metastasis phenotype could be reversed by transfected nm23-H1 cDNA. This study was conducted to transfect nm23-H1 cDNA into L9981 cells and to explore the function of nm23-H1 in reversing the malignant phenotype of L9981 cells. The plasmid of pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and the transfected nm23-H1 cDNA cell lines of L9981-nm23-H1 was established. The protein expression of nm23-H1 was detected by Western blot. The biologic features of L9981-nm23-H1 cells were studied in vitro and in vivo. The results showed that the fusion protein of nm23-H1-EGFP was stable, continuous and expressed with high efficiency in L9981-nm23-H1 cells. The cell proliferation, colon formation and invasive ability are significantly lowered in L9981 cells transfected nm23-H1 cDNA (P < 0.01); the tumorgenesis and the lung metastasis incidence was lower in tranfected nm23-H1 cells than in L9981 and L9981-Plxsn in nude mice (P < 0.01); the rate for inhibiting tumorgenesis of nm23 -H1 was 82.56%. These data suggest that the malignant phenotype could be reversed by wild nm23-H1 gene in L9981 cells.
Che
Guowei
G
The Key Laboratory of Lung Cancer Molecular Biology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Qinghua
Q
Wang
Yanping
Y
Liu
Lunxu
L
Qin
Yang
Y
Sun
Zilin
Z
Sun
Zefang
Z
Chen
Xiaohe
X
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins