title: [Experimental study on the effects of interferon-gamma gene on Tenon's capsule fibroblasts in vitro].
abstract: OBJECTIVE
  To investigate the effects of interferon-gamma (IFN-gamma) gene on Tenon's capsule fibroblasts in vitro.
  
  
  METHODS
  Using lipofectAMINE, IFN-gamma gene was transferred to human Tenon's capsule fibroblasts with plasmid pcDNA3IFN-gamma in vitro. Cells treated with plasmid pcDNA3 without IFN-gamma gene were used as the controls. The transfected fibroblasts were screened by G418. The expression of IFN-gamma was determined by RT-PCR, immunohistochemistry and flow cytometry assay. The effects of IFN-gamma on the proliferation of Tenon's capsule fibroblasts were evaluated by flow cytometry and MTT.
  
  
  RESULTS
  The Tenon's capsule fibroblasts transferred the IFN-gamma gene could express the IFN-gamma RNA and protein transiently. The proliferation of the fibroblasts transferred the IFN-gamma gene was inhibited.
  
  
  CONCLUSIONS
  The proliferation of the Tenon's capsule fibroblasts in vitro can be inhibited by transferred IFN-gamma gene.
authors: Department of Ophthalmology, the Second affiliated Hospital, Zhongshan University, Guangzhou 510120, China.
authors: Yu-qing
authors: YQ
authors: Lan
authors: Jian
authors: J
authors: Ge
authors: Ming-kai
authors: MK
authors: Lin
authors: Jian-liang
authors: JL
authors: Zheng
authors: Jing
authors: J
authors: Wei
authors: Hai-quan
authors: HQ
authors: Liu
authors: Yan-yan
authors: YY
authors: Li
mesh_terms: D003228:Conjunctiva / Q000187:drug effects*; D003238:Connective Tissue / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000235:genetics*; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma