Identification and characterization of Shigella boydii 20 serovar nov., a new and emerging Shigella serotype.
Analysis of 163 putative Shigella isolates from Canada and the USA showed biochemical reactions consistent with Shigella species, although none of the isolates reacted with antiserum raised against any of the well-established or provisional Shigella serotypes. All these isolates, provisionally designated serotype SH108, were positive for the ipaH gene and the invasion-associated locus. All fermented mannitol, were serologically indistinguishable from each other and showed no reaction in antisera prepared against Escherichia coli serotypes O1 to O181. PCR-RFLP analysis of the genes involved in O-antigen synthesis revealed a common pattern among these isolates that was distinct from recognized Shigella serotypes and E. coli. Between 1999 and 2003, isolates from across Canada were submitted to the National Laboratory for Enteric Pathogens for antibiotic susceptibility testing, phage typing and PFGE. These assays revealed heterogeneity among the members of this serotype. Antimicrobial susceptibility testing with seven antibiotics identified six profiles, with 90 % (45/50) of the isolates resistant to four or more antibiotics and 72 % (36/50) resistant to five or more. All isolates were typable using a panel of 16 phages, with 11 different phage types (PTs) represented. The most common PTs found were PT 3 (64 %), PT 6 (10 %) and PT 16 (6 %). Analysis of XbaI-restricted genomic DNA revealed 16 highly related patterns that were not readily distinguishable from those obtained for some other Shigella serotypes. The World Health Organization Collaborating Center for Shigella has added serotype SH108 to the Shigella scheme as S. boydii serotype 20 (serovar nov.). Strain SH108 (isolate 99-4528) is the reference strain for this serotype.
Woodward
David L
DL
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Clark
Clifford G
CG
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Caldeira
Richard A
RA
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Ahmed
Rafiq
R
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Soule
Geoff
G
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Bryden
Louis
L
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Tabor
Helen
H
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Melito
Pasquale
P
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Foster
Roger
R
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Walsh
Julie
J
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Ng
Lai-King
LK
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Malcolm
Georgia B
GB
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Strockbine
Nancy
N
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
Rodgers
Frank G
FG
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
And The Canadian Public Health Laboratory Network
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2 2Bureau of Microbial Hazards, Health Products and Food Branch, Frederick G. Banting Building, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 3Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA 4Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824, USA.
D015373:Bacterial Typing Techniques; D005838:Genotype; D006801:Humans; D019081:O Antigens / Q000032:analysis* / Q000276:immunology; D010641:Phenotype; D012703:Serotyping; D012761:Shigella boydii / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence
D019081:O Antigens