title: Determination of triacylglycerols in donkey milk by using high performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry.
abstract: The separation and determination of triacylglycerols (TAGs), which are the main components of naturally occurring fats and oils, in milk fat is a challenging task due to the very complex nature of this matrix. In the present study the TAG fraction of donkey milk lipids has been characterized by using high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS). HPLC in reversed phase mode has been used for TAG separation and silver ion (Ag+) HPLC has been used as a second dimension to clarify and confirm the identification. The RP-HPLC eluate was fractionated and the fractions of interest were injected onto the Ag+-HPLC column. In both cases peak assignment was carried out by combining retention data with APCI-MS spectra information. In total, 55 TAGs in donkey milk fat were identified (without considering the positional isomers) and quantified on the basis of percentage peak areas in the RP-HPLC chromatogram (without the use of correction factors). Amongst the identified triacylglycerols, POLn, POO, PPO, CaPO, POL, and PPoO proved to be the main components of the TAG fraction of donkey milk.
authors: Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università di Messina, 98166 Messina, Italy.
authors: Paola
authors: P
authors: Dugo
authors: Tiina
authors: T
authors: Kumm
authors: Maria
authors: M
authors: Lo Presti
authors: Biagina
authors: B
authors: Chiofalo
authors: Elisabetta
authors: E
authors: Salimei
authors: Alessia
authors: A
authors: Fazio
authors: Antonella
authors: A
authors: Cotroneo
authors: Luigi
authors: L
authors: Mondello
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018397:Equidae; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D014280:Triglycerides