Expression of Pax group III genes in the honeybee (Apis mellifera).
Pax group III genes are involved in a number of processes during insect segmentation. In Drosophila melanogaster, three genes, paired, gooseberry and gooseberry-neuro, regulate segmental patterning of the epidermis and nervous system. Paired acts as a pair-rule gene and gooseberry as a segment polarity gene. Studies of Pax group III genes in other insects have indicated that their expression is a good marker for understanding the underlying molecular mechanisms of segmentation. We have cloned three Pax group III genes from the honeybee (Apis mellifera) and examined their relationships to other insect Pax group III genes and their expression patterns during honeybee segmentation. The expression pattern of the honeybee homologue of paired is similar to that of paired in Drosophila, but its expression is modulated by anterior-posterior temporal patterning similar to the expression of Pax group III proteins in Tribolium. The expression of the other two Pax group III genes in the honeybee indicates that they also act in segmentation and nervous system development, as do these genes in other insects.
Osborne
Peter W
PW
Laboratory for Development and Evolution, Biochemistry Department, University of Otago, Dunedin, New Zealand.
Dearden
Peter K
PK
D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000196:embryology* / Q000235:genetics*; D019521:Body Patterning / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D040461:Gene Components; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D051761:Paired Box Transcription Factors