title: Effect of glutathione on UV induction of prophage lambda.
abstract: The effect of glutathione (GSH) on the ultraviolet (UV) induction of lambda prophage was investigated in lysogenic Escherichia coli. The data showed that extracellular GSH could inhibit the UV induction of lambda prophage. The inhibitory rates were concentration dependent, and the maximal rate obtained was 94% with 3.0 M GSH. The effect was also measured in three different lambda lysogens: a wild-type strain (wt), an isogenic GSH-deficient strain, and an isogenic strain producing increased amounts of GSH. The result showed that when subjected to UV irradiation (254 nm, 60 J m(-2)), GSH-deficient strain was approximately fivefold more sensitive to be lysed than wt, whereas the strain with higher intracellular GSH levels was only 28% susceptible to be lysed. With electron spin resonance and spin trapping techniques, we observed that free radical signals occurred in the suspensions of UV irradiated lysogenic cells and the intensity of signals was influenced by GSH levels. These results indicate that GSH can significantly inhibit the UV induction of lambda prophage, and that this effect is correlated to its capacity to scavenge free radicals generated after UV irradiation.
authors: College of Life Sciences, Wuhan University, Wuhan, 430072 People's Republic of China.
authors: Yancheng
authors: Y
authors: Liu
authors: Qiuju
authors: Q
authors: Zhang
authors: Chengxiang
authors: C
authors: Fang
authors: Shiqiao
authors: S
authors: Zhu
authors: Yali
authors: Y
authors: Tang
authors: Shaoyi
authors: S
authors: Huang
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000821:virology*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology*; D008242:Lysogeny / Q000187:drug effects; D039002:Prophages / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D005609:Free Radicals; D005978:Glutathione