Dietary soy prevents brain Na+, K(+)-ATPase reduction in streptozotocin diabetic rats.
The aim of this study was to investigate Na+, K(+)-ATPase activity in cerebral cortex, hippocampus and hypothalamus of diabetic rats. The action of dietary soy protein on the effect produced by diabetes on this activity was also tested. Forty-nine-day-old Wistar were divided into two groups: diabetes streptozotocin (50 mg/kg body weight) and control (citrate solution). Rats were sacrificed 56 days later. In other set of experiments, rats received a dietary with casein (control) from day 21 to the 49 of postnatal-age and were subjected to diabetes or received citrate (control). One week later, rats received a special dietary with soy protein with isoflavones or casein (control) from day 56 to the 105 of postnatal-age. Results showed that diabetic rats presented a reduction ( approximately 40%) of Na+, K(+)-ATPase activity in all structures studied. Pretreatment with soy protein prevented the inhibitory effects of diabetes on the enzyme activity. Assuming the possibility that these effects might also occur in the human condition, our findings may be relevant to explain, at least in part, the neurologic dysfunction associated with diabetes and might support a novel therapeutic strategy (soy protein) to slow the progression of neurodegeneration in this disorder.
Franzon
Renata
R
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003 Porto Alegre, RS, Brazil.
Chiarani
Fábria
F
Mendes
Roberta H
RH
Belló-Klein
Adriane
A
Wyse
Angela T S
AT
D000818:Animals; D002364:Caseins; D002462:Cell Membrane / Q000201:enzymology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004044:Dietary Proteins / Q000627:therapeutic use*; D006624:Hippocampus / Q000201:enzymology*; D007031:Hypothalamus / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D030262:Soybean Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D013570:Synaptic Membranes / Q000201:enzymology
D002364:Caseins; D004044:Dietary Proteins; D030262:Soybean Proteins; D000254:Sodium-Potassium-Exchanging ATPase