title: Characterization of beta-hydroxy-beta-methylglutaryl coenzyme A reductase inhibitor from Pueraria thunbergiana.
abstract: This study describes the extraction and characterization of an inhibitor for beta-hydroxy-beta-methylglutaryl (HMG) coenzyme A (CoA) reductase from Pueraria thunbergiana. The maximum HMG-CoA reductase inhibitory activity (IC(50) = 79 microg) was obtained when P. thunbergiana was extracted with 70% ethanol at 30 degrees C for 12 h. After purification of the HMG-CoA reductase inhibitor by means of systematic solvent extraction, silica gel column chromatography, and HPLC, an active fraction with an IC(50) of 0.9 microg (4.25 microM) and a yield of 1.3% was obtained. The purified HMG-CoA reductase inhibitor was identified as daidzein (C(15)H(10)O(4); molecular mass, 254 Da).
authors: Department of Genetic Engineering and Bio-medicinal Resources Research Center and Department of Biology and Medicinal Science, Paichai University, Daejeon 302-735, South Korea.
authors: Hyoung-Jong
authors: HJ
authors: Kim
authors: Dae-Hyoung
authors: DH
authors: Lee
authors: Yoo-Yeon
authors: YY
authors: Hwang
authors: Ki-Sung
authors: KS
authors: Lee
authors: Jong-Soo
authors: JS
authors: Lee
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006224:Cricetinae; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008647:Mesocricetus; D029908:Pueraria / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D000960:Hypolipidemic Agents; D006903:Hydroxymethylglutaryl CoA Reductases