title: A physical map of the genome of Atlantic salmon, Salmo salar.
abstract: A physical map of the Atlantic salmon (Salmo salar) genome was generated based on HindIII fingerprints of a publicly available BAC (bacterial artificial chromosome) library constructed from DNA isolated from a Norwegian male. Approximately 11.5 haploid genome equivalents (185,938 clones) were successfully fingerprinted. Contigs were first assembled via FPC using high-stringency (1e-16), and then end-to-end joins yielded 4354 contigs and 37,285 singletons. The accuracy of the contig assembly was verified by hybridization and PCR analysis using genetic markers. A subset of the BACs in the library contained few or no HindIII recognition sites in their insert DNA. BglI digestion fragment patterns of these BACs allowed us to identify three classes: (1) BACs containing histone genes, (2) BACs containing rDNA-repeating units, and (3) those that do not have BglI recognition sites. End-sequence analysis of selected BACs representing these three classes confirmed the identification of the first two classes and suggested that the third class contained highly repetitive DNA corresponding to tRNAs and related sequences.
authors: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6.
authors: Siemon H S
authors: SH
authors: Ng
authors: Carlo G
authors: CG
authors: Artieri
authors: Ian E
authors: IE
authors: Bosdet
authors: Readman
authors: R
authors: Chiu
authors: Roy G
authors: RG
authors: Danzmann
authors: William S
authors: WS
authors: Davidson
authors: Moira M
authors: MM
authors: Ferguson
authors: Christopher D
authors: CD
authors: Fjell
authors: Bjorn
authors: B
authors: Hoyheim
authors: Steven J M
authors: SJ
authors: Jones
authors: Pieter J
authors: PJ
authors: de Jong
authors: Ben F
authors: BF
authors: Koop
authors: Martin I
authors: MI
authors: Krzywinski
authors: Krzysztof
authors: K
authors: Lubieniecki
authors: Marco A
authors: MA
authors: Marra
authors: Leslie A
authors: LA
authors: Mitchell
authors: Carrie
authors: C
authors: Mathewson
authors: Kazutoyo
authors: K
authors: Osoegawa
authors: Sarah E
authors: SE
authors: Parisotto
authors: Ruth B
authors: RB
authors: Phillips
authors: Matthew L
authors: ML
authors: Rise
authors: Kristian R
authors: KR
authors: von Schalburg
authors: Jacqueline E
authors: JE
authors: Schein
authors: Heesun
authors: H
authors: Shin
authors: Asim
authors: A
authors: Siddiqui
authors: Jim
authors: J
authors: Thorsen
authors: Natasja
authors: N
authors: Wye
authors: George
authors: G
authors: Yang
authors: Baoli
authors: B
authors: Zhu
mesh_terms: D000818:Animals; D020451:Contig Mapping / Q000379:methods; D016172:DNA Fingerprinting; D016678:Genome; D006657:Histones / Q000235:genetics; D008297:Male; D020161:Physical Chromosome Mapping / Q000379:methods* / Q000592:standards; D015183:Restriction Mapping; D020317:Salmo salar / Q000235:genetics*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics
chemical_list: D006657:Histones; C090528:DNA modification methylase HindIII; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)