Toward cocaine esterase therapeutics.
Cocaine is among the most reinforcing of all drugs of abuse, yet no effective pharmacotherapy is available. Herein, we report the development and characterization of phage-displayed cocaine esterases with pharmacologically relevant kinetic parameters (kcat/Km approximately 104 M-1 s-1).
Rogers
Claude J
CJ
The Skaggs Institute for Chemical Biology and Departments of Chemistry and Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Mee
Jenny M
JM
Kaufmann
Gunnar F
GF
Dickerson
Tobin J
TJ
Janda
Kim D
KD
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D001435:Bacteriophages / Q000737:chemistry* / Q000201:enzymology* / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D003042:Cocaine / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D007700:Kinetics
D001426:Bacterial Proteins; D002265:Carboxylic Ester Hydrolases; C019921:cocaine esterase; D003042:Cocaine