Dyella koreensis sp. nov., a beta-glucosidase-producing bacterium.
A bacterial strain (designated BB4(T)), which has beta-glucosidase activity, was isolated from soil around the roots of bamboo plants. Cells were Gram-negative, aerobic, non-motile and straight-rod-shaped. Phylogenetic analysis of 16S rRNA gene sequences revealed a clear affiliation with members of the family 'Xanthomonadaceae'. The 16S rRNA gene sequence of strain BB4(T) showed the following sequence similarities: 97.7% to Dyella japonica XD53(T), 97.1% to Frateuria aurantia LMG 1558(T), 96.2% to Fulvimonas soli LMG 19981(T), 94.3% to Rhodanobacter lindaniclasticus RP5575(T) and <90% to other members of the 'Gammaproteobacteria'. The G+C content of the genomic DNA was 63.8 mol%. The major fatty acids were branched forms, especially large proportions of iso-C(15:0), iso-C(17:0) and iso-C(17:1)omega9c, similar to the profile of the genus Dyella. The results of DNA-DNA hybridization with D. japonica XD53(T) and Frateuria aurantia LMG 1558(T), in combination with phenotypic characteristics and 16S rRNA gene sequence analysis, demonstrated that strain BB4(T) should be classified as a novel Dyella species. The name Dyella koreensis sp. nov. is proposed, with strain BB4(T) (=KCTC 12359(T)=NBRC 100831(T)) as the type strain.
An
Dong-Shan
DS
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, South Korea.
Im
Wan-Taek
WT
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, South Korea.
Yang
Hee-Chan
HC
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, South Korea.
Yang
Deok-Chun
DC
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, South Korea.
Lee
Sung-Taik
ST
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Guseong-dong 373-1, Yuseong-gu, Daejeon 305-701, South Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D044166:Xanthomonadaceae / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001617:beta-Glucosidase / Q000096:biosynthesis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase