Interaction between proteoglycans and alpha-elastin in construction of extracellular matrix of human yellow ligament.
One type of large proteoglycan and three types of small proteoglycans (decorin, decorin-subtype, and biglycan) were purified by chromatography, and alpha-elastin was isolated by alkali treatment from human yellow ligaments taken at the time of operation. The interaction of the proteoglycans with immobilized alpha-elastin on a sensor was analyzed by surface plasmon resonance, and we confirmed that each of the small proteoglycans exhibited a specific binding to alpha-elastin. The binding sites of small proteoglycans were contained in the protein cores. In addition, the differences in the interaction of the small proteoglycans with alpha-elastin of normal and ossified ligaments were compared. The interactions of the small proteoglycans with alpha-elastin of the ossified ligaments were lower than those with alpha-elastin of the normal ligaments. In the ossified ligaments, neodesmosine, one of the cross-linking amino acids, was significantly less than in the normal ligaments (p < .05).
Itabashi
Taito
T
Departments of Biochemistry and Orthopaedic Surgery, Hirosaki University School of Medicine, Hirosaki, Japan. taito46312@nifty.com
Harata
Seiko
S
Endo
Masahiko
M
Takagaki
Keiichi
K
Yukawa
Masahiro
M
Ueyama
Kazumasa
K
Toh
Satoshi
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000032:analysis; D058578:Biglycan; D001665:Binding Sites; D058575:Decorin; D004549:Elastin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000502:physiology*; D016326:Extracellular Matrix Proteins; D006801:Humans; D017843:Ligamentum Flavum / Q000737:chemistry* / Q000166:cytology*; D008875:Middle Aged; D009999:Ossification, Heterotopic / Q000503:physiopathology; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism*; D013131:Spine; D020349:Surface Plasmon Resonance
D000596:Amino Acids; C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D004549:Elastin