title: Cloning and characterization of a phospholipase C-beta isoform from the sea urchin Lytechinus pictus.
abstract: Calcium is a ubiquitous intracellular signaling molecule controlling a wide array of cellular processes including fertilization and egg activation. The mechanism for triggering intracellular Ca(2+) release in sea urchin eggs during fertilization is the generation of inositol-1,4,5-trisphosphate by phospholipase C (PLC) hydrolysis of phosphatidylinositol-4,5-bisphosphate. Of the five PLC isoforms identified in mammals (beta, gamma, delta, epsilon and zeta), only PLCgamma and PLCdelta have been detected in echinoderms. Here, we provide direct evidence of the presence of a PLCbeta isoform, named suPLCbeta, within sea urchin eggs. The coding sequence was cloned from eggs of Lytechinus pictus and determined to have the greatest degree of homology and identity with the mammalian PLCbeta4. The presence of suPLCbeta within the egg was verified using a specifically generated antibody. The majority of the enzyme is localized in the non-soluble fraction, presumably the plasma membrane of the unfertilized egg. This distribution remains unchanged 1 min postfertilization. Unlike PLCbeta4, suPLCbeta is activated by G protein betagamma subunits, and this activity is Ca(2+)-dependent. In contrast to all known PLCbeta enzymes, suPLCbeta is not activated by Galphaq-GTPgammaS subunit suggesting other protein regulators may be present in sea urchin eggs.
authors: Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors: Andre
authors: A
authors: Kulisz
authors: Louisa
authors: L
authors: Dowal
authors: Suzanne
authors: S
authors: Scarlata
authors: Sheldon S
authors: SS
authors: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D047331:Lytechinus / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum / Q000201:enzymology; D054799:Phospholipase C beta; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D010738:Type C Phospholipases / Q000235:genetics* / Q000378:metabolism; D015053:Zygote / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D002118:Calcium