title: [Determination of complanatoside A in semen Astragali complanati by HPLC].
abstract: OBJECTIVE
  To establish the determination method for complanatoside A in seeds of Astragalus complanatus.
  
  
  METHODS
  An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.
  
  
  RESULTS
  The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.
  
  
  CONCLUSIONS
  The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.
authors: Beijing University of Chinese Traditional Medicine, Beijing 100029, China.
authors: Jian-Jun
authors: JJ
authors: Zhang
authors: Xing-Li
authors: XL
authors: Yan
authors: Yu-Jie
authors: YJ
authors: Zhang
authors: Yu-Ping
authors: YP
authors: Wang
authors: Qiu-Ju
authors: QJ
authors: Zhang
authors: Feng-Ying
authors: FY
authors: Zeng
authors: Wei
authors: W
authors: Li
authors: Li-Na
authors: LN
authors: Ou
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044948:Flavonols / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012639:Seeds / Q000737:chemistry
chemical_list: D044948:Flavonols; D005960:Glucosides; C057070:complanatuside