title: A study of the effect of salicylic acetic acid on a lymphocyte cell model of cellular activation and proliferation.
abstract: Salicylic acetic acid (SAA) is a drug that has formed part of the treatment of many diseases for many years. Its anti-inflammatory activity is well known, but recently its possible role in the interference in the oncogenesis mechanisms has become apparent. With the aim of supporting these yet preliminary observations, we studied the effect of salicylic acetic acid on a cellular activation and proliferation model. We used lymphocytes obtained from peripheral blood, which were later exposed to cellular activation and proliferation stimulus by the SEB antigen. Lymphocyte activation was determined by direct immunoflourescence through expression of the receptor IL-2 (CD25) alpha chain and proliferation through the incorporation of tritiated thymidine to the DNA in synthesis together with the determination of the cellular cycle by flow cytometry. We found that both processes, activation and proliferation, are inhibited by increasing doses of SAA.
authors: Medical Oncology Service, Reina Sofia University Hospital, Córdoba, Spain.
authors: Enrique Aranda
authors: EA
authors: Aguilar
authors: Juan
authors: J
authors: de la Haba-Rodríguez
authors: Antonio
authors: A
authors: Macho
authors: Concha
authors: C
authors: Lucena
authors: Auxiliadora
authors: A
authors: Gómez
authors: Marco
authors: M
authors: Calzado
authors: Eduardo
authors: E
authors: Muñoz
mesh_terms: D001241:Aspirin / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004768:Enterotoxins / Q000494:pharmacology; D005434:Flow Cytometry; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D008954:Models, Biological; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D004768:Enterotoxins; D015375:Receptors, Interleukin-2; C031818:enterotoxin B, staphylococcal; D004247:DNA; D001241:Aspirin; D013936:Thymidine