Treponema berlinense sp. nov. and Treponema porcinum sp. nov., novel spirochaetes isolated from porcine faeces.
Limit-dilution procedures were used to isolate seven, helically coiled bacterial strains from faeces of swine that constituted two unidentified taxa. Comparative 16S rRNA gene sequence analysis showed highest similarity values with species of the genus Treponema indicating that the isolates are members of this genus. Strain 7CPL208(T), as well as five further isolates, and 14V28(T) displayed the highest 16S rRNA gene sequence similarities with Treponema pectinovorum ATCC 33768(T) (92.3%) and Treponema parvum OMZ 833(T) (89.9%), respectively. Polar lipid profiles distinguished 7CPL208(T) and 14V28(T) from each other as well as from related species. Based on their phenotypic and genotypic distinctiveness, strains 7CPL208(T) and 14V28(T) are suggested to represent two novel species of the genus Treponema, for which the names Treponema berlinense sp. nov. and Treponema porcinum sp. nov. are proposed. The type strain for Treponema berlinense is 7CPL208(T) (=ATCC BAA-909(T)=CIP 108244(T)=JCM 12341(T)) and for Treponema porcinum 14V28(T) (=ATCC BAA-908(T)=CIP 108245(T)=JCM 12342(T)).
Nordhoff
Marcel
M
Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, D-10115 Berlin, Germany.
Taras
David
D
Institut für Tierernährung, Freie Universität Berlin, D-10115 Berlin, Germany.
Macha
Moritz
M
Institut für Tierernährung, Freie Universität Berlin, D-10115 Berlin, Germany.
Tedin
Karsten
K
Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, D-10115 Berlin, Germany.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Wieler
Lothar H
LH
Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, D-10115 Berlin, Germany.
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine / Q000382:microbiology*; D014208:Treponema / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S