title: [The interaction of the intracellular domain of beta-amyloid precursor protein with JKTBP2].
abstract: APP (beta-amyloid precursor protein) plays an important role in the formation of Alzheimer's Disease (AD), and its proteolytic product, the intracellular domain AID is believed to be involved in this process by promoting cell apoptosis. To further understand the function of AID in the pathology of AD, we utilized AID as a bait to identify AID interacting proteins in a yeast two-hybrid system. One of the positive clones encodes the fragment corresponding to amino acids 90-204 of heterogeneous nuclear ribonucleoprotein D-like JKTBP2. The interaction between JKTBP2(90)-204 and AID was further confirmed by co-immunoprecipitation in the mammalian 293T cells. These results indicate that JKTBP2 may have an important function in AD formation.
authors: Laboratory of Molecular Cell Neurological Sciences, School of Life Sciences, Xiamen University, Xiamen 361005.
authors: Wei Wei
authors: WW
authors: Wang
authors: Zhi Dong
authors: ZD
authors: Li
authors: Run Zhong
authors: RZ
authors: Liu
authors: Sui Gen
authors: SG
authors: Hong
authors: Xua Xi
authors: XX
authors: Xu
authors: Ye Guang
authors: YG
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012261:Ribonucleoproteins / Q000378:metabolism*; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D016564:Amyloid beta-Protein Precursor; C111986:HNRPDL protein, human; D012261:Ribonucleoproteins