title: Effect of cationic surfactants on the interaction between sodium perfluorooctanoate and beta-lactoglobulin.
abstract: The interaction between the fluorocarbon surfactant, sodium perfluorooctanoate (SPFO), and beta-lactoglobulin (BLG) was studied. In particular, the effects of cationic surfactants, such as alkyltriethylammonium bromide (C(n)NE, n=8, 10, 12), on SPFO-BLG interaction were examined. It was shown that the anionic fluorocarbon surfactant, SPFO, was a strong denaturant of BLG. The ability of SPFO to denature BLG could be weakened by the addition of C(n)NE. The effect of C(n)NE on SPFO-BLG interaction was related to the hydrocarbon chain length of C(n)NE, and also the molar ratio of the added C(n)NE to the SPFO in SPFO-BLG solutions ([C(n)NE]/[SPFO]). Our findings might provide a way to design surfactant systems that are less denaturing to proteins or tailor the ability of surfactant to denature proteins through the appropriate mixing with other surfactants.
authors: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
authors: Run-Chao
authors: RC
authors: Lu
authors: Ao-Neng
authors: AN
authors: Cao
authors: Lu-Hua
authors: LH
authors: Lai
authors: Jin-Xin
authors: JX
authors: Xiao
mesh_terms: D002210:Caprylates / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002942:Circular Dichroism; D004553:Electric Conductivity; D005466:Fluorocarbons / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002210:Caprylates; D002412:Cations; D005466:Fluorocarbons; D007782:Lactoglobulins; D013501:Surface-Active Agents; C023036:perfluorooctanoic acid