title: Met-enkephalin involvement in morphine-modulated peritonitis in swiss mice.
abstract: Morphine coinjection with zymosan inhibits pain and leukocyte accumulation during peritonitis in several strains of mice, and affects systems of endogenous opioids. Present investigations focus on Met-enkephalin (Met-ENK) in the inflamed peritoneal cavity and brain centers of Swiss mice. Males of Swiss mice were IP injected with zymosan or zymosan supplemented with morphine. At the selected time points the peritoneal leukocytes were counted and the Met-ENK level was measured in exudatory fluid and leukocytes, striatum, hypothalamus, and pituitary gland. The Met-ENK content in peritoneal fluid rised sharply after zymosan injection, which corresponded with its decline in exudatory leukocytes, hypothalamus, and striatum. Morphine coinjection enhanced intraperitoneal accumulation of Met-ENK and its release from exudatory leukocytes, but inhibited its early fluctuations in hypothalamus and striatum. Effects of morphine-modulated inflammation on the Met-ENK system lasted longer than 7 days.
authors: Department of Evolutionary Immunobiology, Jagiellonian University, Kraków, Poland. chadz@zuk.iz.uj.edu.pl
authors: Magdalena
authors: M
authors: Chadzinska
authors: Anna
authors: A
authors: Scislowska-Czarnecka
authors: Krystyna
authors: K
authors: Pierzchala-Koziec
authors: Barbara
authors: B
authors: Plytycz
mesh_terms: D000701:Analgesics, Opioid / Q000627:therapeutic use*; D000818:Animals; D004744:Enkephalin, Methionine / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology; D008297:Male; D051379:Mice; D009020:Morphine / Q000627:therapeutic use*; D010538:Peritonitis / Q000097:blood / Q000188:drug therapy*; D015054:Zymosan / Q000633:toxicity
chemical_list: D000701:Analgesics, Opioid; D004744:Enkephalin, Methionine; D009020:Morphine; D015054:Zymosan