[Study on analytical method for alkaloids in Bulbus Fritillariae cirrhosae].
OBJECTIVE
To develop a HPLC-ELSD method for the quantitation of peimisine in Bulbus Fritillariae Cirrhosae.


METHODS
HPLC equipped with ELSD was used for the analysis of peimisine in Bulbus Fritillariae Cirrhosae. The separation of peimisine was achieved on a reversed-phase Agilent Hypersil BDS-C18 column with an isocratic phase system consisting of acetonitrile-water-diethylamine (37:63:0. 03) under a flow rate at 1.0 mL x min(-1). Temperature for the detector drift tube was 115 degrees C. The air gas flow rate was 3.0 L x min(-1).


RESULTS
The analysis of peimissine showed good linearity between 0.031 88 - 1.020 mg x mL(-1). The average recovery was 102.1% (n = 5) with RSD 3.86%. According to the results of the 33 different Bulbus Fritillariae Cirrhosae obtained from various origins, the content limit of peimisine was determined to be 0.0070%.


CONCLUSIONS
The assay was successfully utilized to quantify the major biologically active alkaloid in four species of Bulbus Fritillariae Cirrhosae. The developed method is simple, rapid, accurate and sensitive for determining the content of peimisine in crude Bulbus Fritillariae Cirrhosae.
Yu
Hua
H
Key Laboratory of Modern Traditional Chinese Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Jiang
Yan
Y
Li
Ping
P
Li
Shao-Ping
SP
Wang
Yi-Tao
YT
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031410:Fritillaria / Q000737:chemistry* / Q000145:classification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C052510:peimisine