Modification of the synaptic glutamate turnover in the hippocampal tissue exposed to low-frequency, pulsed magnetic fields.
The influence of pulsed magnetic fields (PMF) on the release and uptake of glutamate was investigated. While the release was examined using hippocampal slices, synaptosomes were chosen to characterize the uptake process. (3)H-D-aspartate was used as a marker of glutamergic transmission. The pulsed magnetic fields (9-15 mT) applied according to the pattern which induced epileptic discharges in hippocampus amplified and attenuated the release and uptake of glutamate, respectively. However, the magnetic fields which induced an increase in neuronal excitability without concomitant seizures amplified both processes. These results confirm our previous reports and indicate that the glutamergic synapses are the target of magnetic fields action.
Wieraszko
Andrzej
A
Department of Biology/Program in Neuroscience, The College of Staten Island/CUNY, NY 10314, USA. Wieraszko@mail.csi.cuny.edu
Armani
Jacquline
J
Maqsood
Nida
N
Raja
Haroon
H
Philip
Susan
S
D000818:Animals; D026603:D-Aspartic Acid / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D066298:In Vitro Techniques; D051379:Mice; D009435:Synaptic Transmission / Q000528:radiation effects*; D013574:Synaptosomes / Q000528:radiation effects; D013997:Time Factors; D014316:Tritium / Q000378:metabolism
D014316:Tritium; D018698:Glutamic Acid; D026603:D-Aspartic Acid