Extreme hemodilution with PEG-hemoglobin vs. PEG-albumin.
Isovolemic hemodilution to 11% systemic hematocrit was performed in the hamster window chamber model using 6% dextran 70 kDa (Dx 70) and 5% human serum albumin (HSA). Systemic and microvascular effects of these solutions were compared with polyethylene glycol (PEG)-conjugated 5% albumin (MPA) and PEG-conjugated 4.2% Hb (MP4). These studies were performed for the purpose of comparing systemic and microvascular responses of PEG vs. non-PEG plasma expanders and similar oxygen-carrying vs. noncarrying blood replacement fluids. Mean arterial blood pressure was statistically significantly reduced for all groups compared with baseline (P < 0.05), HSA, MPA, and MP4 higher than Dx 70 (P < 0.05). MP4 and MPA had a significantly higher cardiac index than HSA and Dx 70, in addition to a positive base excess. Microvascular blood flow and capillary perfusion were significantly higher for the PEG compounds compared with HSA and Dx 70. Intravascular PO2 for MP4 and MPA was higher in arterioles (P < 0.05) compared with HSA and Dx 70, but there was no difference in either tissue or venular PO2 between groups. Total Hb in the MP4 group was 4.8 +/- 0.4 g/dl, whereas the remaining groups had a range of 3.6-3.8 g/dl. The hemodilution results showed that PEG compounds maintained microvascular conditions with lower concentrations than conventional plasma expanders. Furthermore, microvascular oxygen delivery and extraction in the window chamber tissue were significantly higher for the PEG compounds. MP4 was significantly higher than MPA (P < 0.05) and was not statistically different from baseline, an effect due to the additional oxygen release to the tissue by the Hb MP4.
Cabrales
Pedro
P
La Jolla Bioengineering Institute, 505 Coast Blvd. S., Suite 405, La Jolla, CA 92037, USA. pcabrales@ucsd.edu
Tsai
Amy G
AG
Winslow
Robert M
RM
Intaglietta
Marcos
M
D000418:Albumins / Q000032:analysis* / Q000737:chemistry*; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001802:Blood Substitutes / Q000032:analysis / Q000737:chemistry; D006224:Cricetinae; D006438:Hemodilution / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D008647:Mesocricetus; D008833:Microcirculation / Q000166:cytology / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*; D011092:Polyethylene Glycols / Q000737:chemistry*
D000418:Albumins; D001802:Blood Substitutes; D006454:Hemoglobins; D011092:Polyethylene Glycols; D010100:Oxygen