title: Localization and role of leptin in the thyroid gland of the lizard Podarcis sicula (Reptilia, Lacertidae).
abstract: Leptin, the product of the ob gene, is a hormone secreted by adipocytes that regulates food intake and energy expenditure. The hypothalamus-pituitary-thyroid axis is markedly influenced by the metabolism status, being suppressed during food deprivation. The present study was designed to ascertain whether (1) lizard thyroid gland expresses the long form of leptin receptor (Ob-Rb) and (2) the leptin administration affects the thyroid gland activity in this species (and to verify whether leptin plays a similar role in reptiles as observed in the other vertebrates). The presence of leptin receptor in the thyroid gland of Podarcis sicula was demonstrated by immunohistochemical technique (avidin-biotin-peroxidase complex--ABC method). The role of leptin in the control of thyroid gland activity was studied in vivo using light microscopy (LM) technique coupled to a specific radioimmunoassay for thyroid-stimulating hormone (TSH) and thyroid hormones (T4 and T3). Leptin (0.1 mg/100 g body wt)/day increased T4 and T3 release for 3 days but decreased the plasma concentration of TSH; using LM clear signs of stimulation in the thyroid gland were observed. These findings suggest that systemic administration of leptin stimulates the morphophysiology of the thyroid gland in the lizard through a direct mechanism involving Ob-Rb.
authors: Dipartimento di Scienze Biologiche ed Ambientali, Università degli Studi del Sannio, Via Port'Arsa 11, I-82100 Benevento, Italy. sciarrillo@unisannio.it
authors: Rosaria
authors: R
authors: Sciarrillo
authors: Francesca
authors: F
authors: Virgilio
authors: Maria
authors: M
authors: De Falco
authors: Vincenza
authors: V
authors: Laforgia
authors: Lorenzo
authors: L
authors: Varano
authors: Marina
authors: M
authors: Paolucci
mesh_terms: D000818:Animals; D020738:Leptin / Q000494:pharmacology / Q000502:physiology*; D008116:Lizards / Q000502:physiology*; D008297:Male; D011956:Receptors, Cell Surface / Q000502:physiology; D054411:Receptors, Leptin; D030162:Reptilian Proteins / Q000502:physiology*; D013961:Thyroid Gland / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013972:Thyrotropin / Q000502:physiology; D013974:Thyroxine / Q000502:physiology; D014284:Triiodothyronine / Q000502:physiology
chemical_list: D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D030162:Reptilian Proteins; D014284:Triiodothyronine; D013972:Thyrotropin; D013974:Thyroxine