Specific transport of S-nitrosocysteine in human red blood cells: Implications for formation of S-nitrosothiols and transport of NO bioactivity within the vasculature.
The transport of various S-nitrosothiols, NO and NO donors in human red blood cells (RBC) and the formation of erythrocytic S-nitrosoglutathione were investigated. Of the NO species tested only S-nitrosocysteine was found to form S-nitrosoglutathione in the RBC cytosol. L-Serine, L-cysteine and L-lysine inhibited formation of S-nitrosoglutathione. Incubation of RBC pre-incubated with S-[15N]nitroso-L-cysteine with native plasma or platelet-rich plasma led to formation of S-[15N]nitrosoalbumin and inhibited platelet aggregation, respectively. The specific transporter system of S-nitroso-L-cysteine in the RBC membrane may have implications for formation of S-nitrosoalbumin and S-nitrosohemoglobin and for transport of NO bioactivity within the vasculature.
Sandmann
Jörg
J
Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30623 Hannover, Germany.
Schwedhelm
Kathrin S
KS
Tsikas
Dimitrios
D
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D001808:Blood Vessels / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009569:Nitric Oxide / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects; D026403:S-Nitrosothiols / Q000097:blood / Q000378:metabolism*
D000596:Amino Acids; D026403:S-Nitrosothiols; D009569:Nitric Oxide; D000244:Adenosine Diphosphate; C021315:S-nitrosocysteine; D003545:Cysteine