Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis.
Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome.
Battistoni
Federico
F
Laboratory of General Microbiology, University Bremen, P.O. Box 33 04 40, D-28334 Bremen, Germany.
Bartels
Daniela
D
Kaiser
Olaf
O
Marie Reamon-Buettner
Stella
S
Hurek
Thomas
T
Reinhold-Hurek
Barbara
B
D020627:Azoarcus / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D005822:Genetic Vectors; D016680:Genome, Bacterial; D008970:Molecular Weight; D009876:Operon / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal