Integrated RF probe for in vivo multinuclear spectroscopy and functional imaging of rat brain using an 11.7 Tesla 89 mm bore vertical microimager.
To acquire high quality in vivo NMR data from rat brain using a vertical 89-mm bore magnet, specially designed NMR probes with integrated RF coils and animal handling capability are required. An RF probe design that is also capable of rat head fixation, body support and suitable for physiology monitoring and maintenance was constructed for an 89 mm bore, 11.7 T, vertical microimager which is equipped with a 57-mm i.d. gradient insert. Design concept and practical aspects of probe construction are described in detail. The device allows accurate and highly reproducible positioning of rat head inside the magnet while providing excellent RF performance. Typical results from fMRI, localized in vivo proton and multinuclear spectroscopy using this probe system are presented.
Li
S
S
NIMH Magnetic Resonance Spectroscopy Core Facility, National Institute of Mental Health, National Institute of Health, 9000 Rockville Pike, Bethesda, MD 20892-1527, USA.
Shen
J
J
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation* / Q000379:methods; D011522:Protons; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration
D018377:Neurotransmitter Agents; D011522:Protons; D005947:Glucose