title: SCL, GATA-2 and Lmo2 expression in neurogenesis.
abstract: SCL, Lmo2 and GATA factors form common transcription complexes during hematopoietic differentiation. The overlapping expression of SCL with GATA-2 and GATA-3 in the developing brain indicated that these factors might collaborate also in the course of neural tissue differentiation. The expression pattern of Lmo2 in the developing CNS, however, is not well understood. Here, we show that neural cells in the early embryonic chick mid- and hindbrain express SCL and GATA-2, while Lmo2 is expressed only in vascular elements. The lack of Lmo2 transcripts in neural cells demonstrated that SCL and GATA-2 cannot form common complexes with Lmo2 in the developing brain. In the course of neural tissue genesis, GATA-2 mRNA appeared prior to the SCL transcript. While GATA-2 expression decreased with maturation, SCL expression persisted at a high level also in post-neurogenic periods. The temporal pattern of SCL and GATA-2/3 expression was investigated also in vitro, in the course of induced neurogenesis by NE-4C neural stem cells. While GATA-2 expression increased from the very beginning of differentiation, SCL expression appeared only in more differentiated cells expressing proneural genes. GATA-3 expression, on the other hand, was detected only in advanced stages of the neuronal maturation, which were characterised by the activation of the Math2 neuronal gene. Similarly to the hematopoietic differentiation, GATA-2 expression precedes the activation of both SCL and GATA-3, and may play roles in the activation of the SCL gene in neuronal development. In contrast to hematopoietic differentiation, however, our results failed to demonstrate co-assembling of GATA factors or SCL with Lmo2. While overlapping expression of GATA-2/3 and SCL was detected, Lmo2 activation could not be demonstrated in neural cells in the investigated period of neuronal development.
authors: Institute of Experimental Medicine of Hungarian Academy of Sciences, Neural Cell Biology Group, Szigony u. 43, 1083 Budapest, Hungary. herberth@koki.hu
authors: B
authors: B
authors: Herberth
authors: K
authors: K
authors: Minkó
authors: A
authors: A
authors: Csillag
authors: T
authors: T
authors: Jaffredo
authors: E
authors: E
authors: Madarász
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D050989:GATA2 Transcription Factor; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008667:Metalloproteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; D008667:Metalloproteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors