Radical scavenging activity and stability of betalains from Beta vulgaris hairy root culture in simulated conditions of human gastrointestinal tract.
Stability and radical scavenging activity of betalains, biosynthesized by Beta vulgaris hairy root culture were investigated at different pH values, bile salts concentration, as well as at the in vitro conditions of gastrointestinal tract. It was established that pH below 3 and concentrations of the bile salts up to 4% had no great influence on betalains stability. At the in vitro conditions of gastrointestinal tract betalains are relatively stable, as their radical scavenging activity decrease from 75% inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH*) to about 38%.
Pavlov
Atanas
A
Technische Universität Dresden, Institut für Lebensmittel und Bioverfahrnstechnik, Mommsenstrasse 13, 01069 Dresden, Germany. Atanas.Pavlov@mailbox.tu-dresden.de
Kovatcheva
Petia
P
Tuneva
Dimka
D
Ilieva
Mladenka
M
Bley
Thomas
T
D027461:Beta vulgaris / Q000737:chemistry*; D050858:Betalains; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000378:metabolism*; D005766:Gastrointestinal Contents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*
D016166:Free Radical Scavengers; D000644:Quaternary Ammonium Compounds; D050858:Betalains