title: Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function.
abstract: In this paper, we aimed to study the role of mannose receptor (MR) in oligochitosan induced RAW264.7 (a murine macrophage cell line) activation. Oligochitosan, which has 3-10 saccharide (N-acetyl-glucosamine or glucosamine) residues, was prepared by enzymatic hydrolysis of chitosan using cellulase. Fluorophore 2-aminoacridone was conjugated to oligochitosan to observe cellular events of oligochitosan-RAW264.7 interaction under Confocal Laser Microscopy. RT-PCR was performed to assess the level of tumor necrosis factor-alpha (TNF-alpha) secretion. Free cytosolic Ca(2+) was measured using the fluorescent Ca(2+) indicator Fluo3/AM. We found that MR was the major receptor responsible for oligochitosan uptake. MR was also engaged in oligochitosan induced TNF-alpha enhancement and [Ca(2+)](in) flux and may serve as a signaling receptor in oligochitosan-induced activation of macrophage function.
authors: Department of Biochemistry and Molecular Biology, Medical College of Zhejiang University, Hangzhou, PR China.
authors: Yanping
authors: Y
authors: Han
authors: Luhang
authors: L
authors: Zhao
authors: Zhijun
authors: Z
authors: Yu
authors: Jie
authors: J
authors: Feng
authors: Qiqi
authors: Q
authors: Yu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D005786:Gene Expression Regulation; D037181:Lectins, C-Type / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D051379:Mice; D009844:Oligosaccharides; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan; D002118:Calcium