Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.).
A mapping population of F(8)derived recombinant inbred lines (RILs) was established from a cross between a domesticated breeding line 83A:476 and a wild type P27255 in narrow-leaf lupin (Lupinus angustifolius L.). The parents together with the 89 RILs were subjected to DNA fingerprinting using microsatellite-anchored fragment length polymorphism (MFLP) to rapidly generate DNA markers to construct a linkage map. Five hundred and twenty two unique markers of which 21% were co-dominant, were generated and mapped. Phenotypic data for the domestication traits: mollis (soft seeds), leucospermus (white flower and seed colour); Lentus (reduced pod-shattering), iucundis (low alkaloid), Ku (early flowering) and moustache pattern on seed coats; were included. Three to 7 molecular markers were identified within 5 cM of each of these domestication genes. The anthracnose resistance gene Lanr1 was also mapped. Linkage groups were constructed using MapManager version QTXb20, resulting in 21 linkage groups consisting of 7 or more markers. The total map length was 1543 cM, with an average distance of 3.4 cM between adjacent markers. This is the first published map for a lupin species. The map can be exploited for marker assisted selection for genetic improvement in lupin breeding programs.
Boersma
Jeffrey G
JG
Department of Agriculture, Western Australia, 3 Baron-Hay Court, South Perth, W.A. 6151, Australia. jboersma@agric.wa.gov.au
Pallotta
Margaret
M
Li
Chengdao
C
Buirchell
Bevan J
BJ
Sivasithamparam
Krishnapillai
K
Yang
Huaan
H
D002874:Chromosome Mapping; D020231:Colletotrichum / Q000472:pathogenicity; D018556:Crops, Agricultural / Q000235:genetics*; D004351:Drug Resistance; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D008126:Lod Score; D031297:Lupinus / Q000033:anatomy & histology / Q000235:genetics*; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000235:genetics; D011110:Polymorphism, Genetic
D005819:Genetic Markers