[The interaction of the intracellular domain of beta-amyloid precursor protein with JKTBP2].
APP (beta-amyloid precursor protein) plays an important role in the formation of Alzheimer's Disease (AD), and its proteolytic product, the intracellular domain AID is believed to be involved in this process by promoting cell apoptosis. To further understand the function of AID in the pathology of AD, we utilized AID as a bait to identify AID interacting proteins in a yeast two-hybrid system. One of the positive clones encodes the fragment corresponding to amino acids 90-204 of heterogeneous nuclear ribonucleoprotein D-like JKTBP2. The interaction between JKTBP2(90)-204 and AID was further confirmed by co-immunoprecipitation in the mammalian 293T cells. These results indicate that JKTBP2 may have an important function in AD formation.
Wang
Wei Wei
WW
Laboratory of Molecular Cell Neurological Sciences, School of Life Sciences, Xiamen University, Xiamen 361005.
Li
Zhi Dong
ZD
Liu
Run Zhong
RZ
Hong
Sui Gen
SG
Xu
Xua Xi
XX
Chen
Ye Guang
YG
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012261:Ribonucleoproteins / Q000378:metabolism*; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
D016564:Amyloid beta-Protein Precursor; C111986:HNRPDL protein, human; D012261:Ribonucleoproteins