title: Adenosine A2A and dopamine D2 heteromeric receptor complexes and their function.
abstract: The existence of A2A-D2 heteromeric complexes is based on coimmunoprecipitation studies and on fluorescence resonance energy transfer and bioluminescence resonance energy transfer analyses. It has now become possible to show that A2A and D2 receptors also coimmunoprecipitate in striatal tissue, giving evidence for the existence of A2A-D2 heteromeric receptor complexes also in rat striatal tissue. The analysis gives evidence that these heteromers are constitutive, as they are observed in the absence of A2A and D2 agonists. The A2A-D2 heteromers could either be A2A-D2 heterodimers and/or higher-order A2A -D2 hetero-oligomers. In striatal neurons there are probably A2A-D2 heteromeric complexes, together with A2A-D2 homomeric complexes in the neuronal surface membrane. Their stoichiometry in various microdomains will have a major role in determining A2A and D2 signaling in the striatopallidal GABA neurons. Through the use of D2/D1 chimeras, evidence has been obtained that the fifth transmembrane (TM) domain and/or the I3 of the D2 receptor are part of the A2A-D2 receptor interface, where electrostatic epitope-epitope interactions involving the N-terminal part of I3 of the D2 receptor (arginine-rich epitope) play a major role, interacting with the carboxyl terminus of the A2A receptor. Computerized modeling of A2A-D2 heteromers are in line with these findings. It seems likely that A2A receptor-induced reduction of D2 receptor recognition, G protein coupling, and signaling, as well as the existence of A2A-D2 co-trafficking, are the consequence of the existence of an A2A-D2 receptor heteromer. The relevance of A2A-D2 heteromeric receptor complexes for Parkinson's disease and schizophrenia is emphasized as well as for the treatment of these diseases. Finally, recent evidence for the existence of antagonistic A2A-D3 heteromeric receptor complexes in cotransfected cell lines has been summarized.
authors: Department of Neuroscience, Division of Cellular and Molecular Neurochemistry, Karolinska Institutet, Stockholm, Sweden. Kjell.Fuxe@neuro.ki.se
authors: Kjell
authors: K
authors: Fuxe
authors: Sergi
authors: S
authors: Ferré
authors: Meritxell
authors: M
authors: Canals
authors: Maria
authors: M
authors: Torvinen
authors: Anton
authors: A
authors: Terasmaa
authors: Daniel
authors: D
authors: Marcellino
authors: Steven R
authors: SR
authors: Goldberg
authors: William
authors: W
authors: Staines
authors: Kirsten X
authors: KX
authors: Jacobsen
authors: Carmen
authors: C
authors: Lluis
authors: Amina S
authors: AS
authors: Woods
authors: Luigi F
authors: LF
authors: Agnati
authors: Rafael
authors: R
authors: Franco
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000503:physiopathology; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D043705:Receptor, Adenosine A2A / Q000737:chemistry / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012559:Schizophrenia / Q000188:drug therapy / Q000503:physiopathology
chemical_list: D014150:Antipsychotic Agents; D046911:Macromolecular Substances; D043705:Receptor, Adenosine A2A; D017448:Receptors, Dopamine D2; D011994:Recombinant Proteins