title: Mineralization of SaOS-2 cells on enzymatically (silicatein) modified bioactive osteoblast-stimulating surfaces.
abstract: There is a demand for novel bioactive supports in surgery, orthopedics, and tissue engineering. The availability of recombinant silica-synthesizing enzyme (silicatein) opens new possibilities for the synthesis of silica-containing bioactive surfaces under ambient conditions that do not damage biomolecules like proteins. Here it is shown that growth of human osteosarcoma SaOS-2 cells on cluster plates precoated with Type 1 collagen is not affected by additional coating of the plates with the recombinant silicatein and incubation with its enzymatic substrate, tetraethoxysilane (TEOS). However, the enzymatic modification of the plates by biosilica deposition on the protein-coated surface caused a marked increase in calcium phosphate formation of SaOS-2 cells as revealed by alizarin red-S staining to quantify calcium mineral content. The increased occurrence of calcium-phosphate nodules on the modified surface was also observed by scanning electron microscopy. These results suggest that by supporting calcium-phosphate deposition in vitro, biosilica (silicatein)-modified surfaces are potentially bioactive in vivo, by stimulating osteoblast mineralization function.
authors: Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
authors: Heinz C
authors: HC
authors: Schröder
authors: Oleksandra
authors: O
authors: Boreiko
authors: Anatoli
authors: A
authors: Krasko
authors: Andreas
authors: A
authors: Reiber
authors: Heiko
authors: H
authors: Schwertner
authors: Werner E G
authors: WE
authors: Müller
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism; D002113:Calcification, Physiologic / Q000502:physiology*; D002130:Calcium Phosphates / Q000378:metabolism; D002403:Cathepsins / Q000502:physiology* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000502:physiology; D006801:Humans; D010006:Osteoblasts / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000378:metabolism / Q000648:ultrastructure; D012821:Silanes / Q000378:metabolism; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D024042:Collagen Type I; D011994:Recombinant Proteins; D012821:Silanes; C112571:silicatein alpha; C040733:tetraethoxysilane; C020243:calcium phosphate; D002403:Cathepsins