title: Core binding factor in the early avian embryo: cloning of Cbfbeta and combinatorial expression patterns with Runx1.
abstract: We have isolated the avian ortholog for CBFbeta, the common non-DNA binding subunit of the core binding factor (CBF) that has important regulatory roles in major developmental pathways. CBFbeta forms heterodimers with the DNA-binding Runx proteins and increases their affinity for DNA and their protein stability. Here, we describe the Cbfbeta expression pattern during the first 4 days of chick embryo development, with a special interest in the developing hematopoietic system. We have compared its expression pattern to that of Runx1, which is crucial for the generation of definitive hematopoietic cells, and to other hematopoietic- or endothelial-specific markers (c-Myb, Pu.1, CD45, c-Ets-1 and VE-Cadherin). Initially, Cbfbeta is widely expressed in the early mesoderm in both the yolk sac and the embryo proper, but later its expression becomes restricted to specific organs or cell types. We have found that Cbfbeta expression overlaps with Runx1 in the hematopoietic system and neural tube. The somitic and mesonephric structures, however, express Cbfbeta in the absence of detectable Runx1. Finally, Cbfbeta and Runx1 display multiple combinatorial patterns in the endoderm and in specific nerves or ganglia. Taken together, we show that Cbfbeta exhibits a dynamic expression pattern that varies according to the organ, cell type or developmental stage. By revealing multiple combinatorial patterns between Cbfbeta and Runx1, these data provide new insights into the role of CBF during early development.
authors: UMR7622, UMPC Paris VI, 9, Quai St Bernard, 75005 Paris, France.
authors: K
authors: K
authors: Bollerot
authors: S
authors: S
authors: Romero
authors: D
authors: D
authors: Dunon
authors: T
authors: T
authors: Jaffredo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo / Q000737:chemistry / Q000378:metabolism*; D002645:Chickens / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D003001:Cloning, Molecular; D050676:Core Binding Factor Alpha 2 Subunit / Q000032:analysis / Q000378:metabolism*; D050658:Core Binding Factor beta Subunit / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D019170:Somites / Q000737:chemistry / Q000378:metabolism
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D050658:Core Binding Factor beta Subunit; D018076:DNA, Complementary; D012333:RNA, Messenger