Cyanovirin-N binding to Manalpha1-2Man functionalized dendrimers.
Manalpha1-2Man functionalized G(3) and G(4)-PAMAM dendrimers have been synthesized and characterized by MALDI-TOF MS and NMR spectroscopy. Precipitation assays to assess the binding of the dimannose-functionalized dendrimers to Cyanovirin-N, a HIV-inactivating protein that blocks virus-to-cell fusion through high mannose mediated interactions, are presented.
Mangold
Shane L
SL
Department of Chemistry and Biochemistry and Center for Bioinspired Nanomaterials, 108 Gaines Hall, Montana State University, Bozeman, MT 59717, USA.
Morgan
Joel R
JR
Strohmeyer
Gregory C
GC
Gronenborn
Angela M
AM
Cloninger
Mary J
MJ
D001426:Bacterial Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000737:chemistry*; D006678:HIV / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry*; D008561:Membrane Fusion / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D002352:Carrier Proteins; C107733:cyanovirin N; D008358:Mannose