Stimulation of reparative osteogenesis after xenotransplantation of human prenatal mesenchymal stem cells and chondroblasts.
Regeneration of the bone tissue after unilateral xenogeneic transplantation of a suspension of human mesenchymal cells and chondroblasts was studied in rats with experimental damage to both femurs. The state of animals was satisfactory and non-depressed in the early and late postoperation period. No local pathological reactions and complications were seen. Administration of mesenchymal stem cells and chondroblasts into the area of bone defect accelerated regeneration on days 10 and 30 (compared to the control group). Implantation of mesenchymal stem cells more significantly stimulated reparative osteogenesis compared to treatment with chondroblasts. This was mainly associated with increased ratio of mature lamellar bone tissue. Unilateral transplantation of the cell suspension stimulated regeneration in the contralateral limb due to accelerated maturation of the bone tissue. The bone tissue formed after transplantation of mesenchymal stem cells and chondroblasts was integrated into bone organ and underwent complete remodeling. Xenotransplantation of prenatal mesenchymal stem cells and chondroblasts without immunosuppression was not followed by the development of early and delayed complications or local reactions of graft rejection.
Fatkhudinov
T Kh
TKh
Institute of Stem Cell and Cell Technologies, Russian Academy of Medical Sciences, Russia. fatkhudinov@iseet.ru
Gol'dshtein
D V
DV
Kulakov
A A
AA
Grigor'yan
A S
AS
Pulin
A A
AA
Makarov
A V
AV
Shamenkov
D A
DA
Rzhaninova
A A
AA
Gornostaeva
S A
SA
D019902:Chondrocytes / Q000637:transplantation*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D010012:Osteogenesis; D014183:Transplantation, Heterologous