title: Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.
abstract: The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.
authors: The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masasato@is.icc.u-tokai.ac.jp
authors: Masahiro
authors: M
authors: Sato
authors: Masato
authors: M
authors: Ohtsuka
authors: Yuhsuke
authors: Y
authors: Ohmi
mesh_terms: D017103:Bacillus Phages / Q000235:genetics*; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D004996:Ethidium / Q000494:pharmacology; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D012984:Software; D013698:Templates, Genetic; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D004270:DNA, Circular; D049452:Green Fluorescent Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004996:Ethidium