Identification of mast cell progenitors in adult mice.
It is well known that mast cells are derived from hematopoietic stem cells. However, in adult hematopoiesis, a committed mast cell progenitor has not yet been identified in any species, nor is it clear at what point during adult hematopoiesis commitment to the mast cell lineage occurs. We identified a cell population in adult mouse bone marrow, characterized as Lin(-)c-Kit(+)Sca-1(-)-Ly6c(-)FcepsilonRIalpha(-)CD27(-)beta7(+)T1/ST2+, that gives rise only to mast cells in culture and that can reconstitute the mast cell compartment when transferred into c-kit mutant mast cell-deficient mice. In addition, our experiments strongly suggest that these adult mast cell progenitors are derived directly from multipotential progenitors instead of, as previously proposed, common myeloid progenitors or granulocyte/macrophage progenitors.
Chen
Ching-Cheng
CC
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305-5324, USA.
Grimbaldeston
Michele A
MA
Tsai
Mindy
M
Weissman
Irving L
IL
Galli
Stephen J
SJ
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D017931:DNA Primers; D016650:Fluorescein-5-isothiocyanate; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D019009:Proto-Oncogene Proteins c-kit; D016650:Fluorescein-5-isothiocyanate