The protective action of alpha-tocopherol on the white matter lipids during moderate hypoxia in rats.
Hypoxia and ischemia acting on the brain cause alterations of the level of lipids and sterols. Famile 3.0-3.5-month-old rats were used for the experiment. They were given alpha-tocopherol in the dose of 11.43 mg/kg of body weight through seven days, then underwent hypoxia (7% of oxygen in the breathing mixture) and myelin was isolated in four times after experiment: 4, 24 hours, 14 days and 2 months after experiment. Three lipids groups were isolated that are neutral lipids, galactolipids and phospholipids. They were quantitatively analyzed with spectrophotocolorimetry. The obtained results indicate that vitamin E administration to animals does not cause significant changes of brain lipids levels. However, alpha-tocopherol administred before moderate hypoxia balances the concentrations of lisophosphatidylcholine and phosphatidylinositide and cerebrosides with control level 2 months after experiment. Vitamin E changes in concentration of the myelin neutral lipids. Vitamin E administered before experimental moderate hypoxia stabilizes some membrane lipids and could be used in brain hypoxia.
Kapelusiak-Pielok
Magdalena
M
Laboratory of Neurobiology, Department of Neurology, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland.
Adamczewska-Goncarzewicz
Zofia
Z
Dorszewska
Jolanta
J
Grochowalska
Alina
A
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003124:Colorimetry; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000517:prevention & control*; D008055:Lipids / Q000032:analysis*; D009186:Myelin Sheath / Q000737:chemistry / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry; D024502:alpha-Tocopherol / Q000494:pharmacology*
D008055:Lipids; D018696:Neuroprotective Agents; D024502:alpha-Tocopherol