title: Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells.
abstract: OBJECTIVE
  To induce apoptosis of endometriotic cells of patients with endometriosis.
  
  
  METHODS
  To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis.
  
  
  METHODS
  University hospital infertility center.
  
  
  METHODS
  Twelve women who visited the center for infertility therapy.
  
  
  METHODS
  Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment.
  
  
  METHODS
  Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining.
  
  
  RESULTS
  The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 microg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 microg of polyglycolic acid/chitosan glue A.
  
  
  CONCLUSIONS
  This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy.
authors: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors: Yu-Chi
authors: YC
authors: Wang
authors: Ru-Huei
authors: RH
authors: Fu
authors: Hsyue-Jen
authors: HJ
authors: Hsieh
authors: Hsiang-Tai
authors: HT
authors: Chao
authors: Shu-Huei
authors: SH
authors: Kao
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000473:pathology; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D011100:Polyglycolic Acid / Q000494:pharmacology*
chemical_list: D011100:Polyglycolic Acid; D048271:Chitosan