Changed course of cytotoxic reaction as a result of modification of target cell membrane.
We studied the sensitivity of K-562 erythromyeloblasts cultured for a long time in a nutrient media of common composition (control) or medium modified by proline-rich hexapeptide, to cytolysis mediated by natural killers. On day 2 of culturing the cytotoxicity of human natural killers towards targets maintained in the modified medium was almost 2-fold higher than in the control. By day 30 this difference was retained at the effector:target ratio of 50:1 and 12:1. By day 90 of the experiment activity of natural killers towards K-562 cells cultured in modified medium was lower than in the control. The results are discussed in the context of hydration of the target cell surface structures at the expense of bound water brought by proline-rich open type sequences into the distal regions of extracellular sites of adhesion receptors and target determinants recognized by natural killers.
Cheknev
C B
CB
Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences. cheknev@riem.ru
Gorozhanina
E S
ES
D000328:Adult; D002462:Cell Membrane / Q000276:immunology*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D017077:Culture Media, Conditioned; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010455:Peptides; D011392:Proline / Q000737:chemistry; D013997:Time Factors
D003470:Culture Media; D017077:Culture Media, Conditioned; D010455:Peptides; D011392:Proline