title: Gene expression of the repulsive guidance molecules during development of the mouse intestine.
abstract: Repulsive guidance molecules (RGMs) are recently identified proteins implicated in neuronal differentiation, migration, and apoptosis. However, in non-neural tissues a specific biological function of RGM is still unknown. In this study, we describe the expression patterns of the RGM members (a, b, and c) during embryonic and postnatal development of the small and large murine intestine. We demonstrated by RT-PCR, in situ hybridization, Western blot, and immunocytochemistry that subtypes RGMa and RGMb but not RGMc were strongly expressed in enteric ganglia cells of the fetal and adult gut. In contrast to the enteric nervous system, RGMa and RGMb expression in the intestinal epithelium started during postnatal gut development. Interestingly, both subtypes were predominantly expressed in the proliferative crypt compartment of the gut epithelium and in paneth cells of small intestine. The development-dependent expression in enteric ganglia and intestinal epithelial cells suggests that RGM may be involved in cell migration, differentiation, and apoptosis with similar cellular mechanisms as described in the central nervous system.
authors: Institute of Anatomy, Department of Experimental Embryology, Division of Tissue Engineering, University of Tübingen, Germany.
authors: Marco
authors: M
authors: Metzger
authors: Sabine
authors: S
authors: Conrad
authors: Gonzalo
authors: G
authors: Alvarez-Bolado
authors: Thomas
authors: T
authors: Skutella
authors: Lothar
authors: L
authors: Just
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D005724:Ganglia / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007422:Intestines / Q000196:embryology*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071020:Hemochromatosis Protein
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C483496:Rgma protein, mouse; C483497:Rgmb protein, mouse; C091279:neogenin; C504414:HJV protein, mouse; D000071020:Hemochromatosis Protein