Application of triazine "superactive esters" in the repetitive synthesis of oligopeptides. Part 1. Synthesis of [54-59] fragment of human beta-casein.
A new synthetic protocol which considerably improves the classic REMA procedure is proposed herein. The offered modification is based on the application of triazine "superactive esters" as the superior substitutes for mixed anhydrides, which have been used as acylating reagent in the classic procedure. The improved repetitive procedure in solution was applied to the preparation of [54-59] fragment of human beta-casein. The structure and high purity of the intermediates, as well as of the final products, was confirmed by FAB-MS, 1H-NMR and HPLC.
Kamiński
Zbigniew J
ZJ
Institute of Organic Chemistry, Technical University of Lódź, 116 Zeromskiego Str., 90-924 Lódź, Poland. kaminsz@mail.p.lodz.pl
Saleh
Bashar
B
Kolesińska
Beata
B
Redliński
Adam
A
Rudziński
Juliusz
J
D000812:Anhydrides; D002364:Caseins / Q000138:chemical synthesis*; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009842:Oligopeptides / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000138:chemical synthesis; D014227:Triazines / Q000737:chemistry*
D000812:Anhydrides; D002364:Caseins; D007202:Indicators and Reagents; D009842:Oligopeptides; D010446:Peptide Fragments; D014227:Triazines