Angiogenic response induced by acellular femoral matrix in vivo.
We investigated the angiogenic response induced by acellular femoral matrices implanted in vivo on to the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. The results showed that acellular matrices were able to induce a strong angiogenic response, comparable with that of fibroblast growth factor-2 (FGF-2), a well-known angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta-1 (TGF-beta1) was added to the matrices and inhibited by the addition of anti-FGF-2 or anti-TGF-beta1 antibodies. The response may be considered to be dependent on a direct angiogenic effect exerted by the matrices, and also in part by the presence of FGF-2 and TGF-beta1 in the acellular matrices.
Conconi
Maria Teresa
MT
Department of Pharmaceutical Sciences, University of Padua, Italy.
Nico
Beatrice
B
Rebuffat
Piera
P
Crivellato
Enrico
E
Parnigotto
Pier Paolo
PP
Nussdorfer
Gastone G
GG
Ribatti
Domenico
D
D043925:Angiogenesis Inducing Agents / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D005109:Extracellular Matrix / Q000502:physiology / Q000637:transplantation*; D005269:Femur; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007150:Immunohistochemistry / Q000379:methods; D018919:Neovascularization, Physiologic; D051381:Rats; D046509:Tissue Culture Techniques; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
D043925:Angiogenesis Inducing Agents; D000911:Antibodies, Monoclonal; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2