Novel synthesis and characterization of bioconjugate block copolymers having oligonucleotides.
Novel and efficient synthesis of polymers terminated with nucleotides via the phosphoramidite method has been developed. A hydroxyl-terminated polymer was converted into a polymer capped with a nucleotide in three steps, where the conversion of the reactions was very high, almost 100%. By repetition of this synthetic method, a block copolymer composed of a synthetic polymer, polystyrene, and biological oligonucleotides with thymidine units has been successfully synthesized. A microphase-separated structure of this block copolymer was observed by both transmission electron microscopy and small-angle X-ray scattering, and a cylindrical structure was confirmed.
Noro
Atsushi
A
Department of Applied Chemistry, Graduate School of Engineering, and Laboratory of Bioorganic Chemistry, Graduate School of Information Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Nagata
Yutaka
Y
Tsukamoto
Masaki
M
Hayakawa
Yoshihiro
Y
Takano
Atsushi
A
Matsushita
Yushu
Y
D001704:Biopolymers / Q000737:chemistry*; D002850:Chromatography, Gel; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D009841:Oligonucleotides / Q000737:chemistry*; D013942:Thymine Nucleotides / Q000737:chemistry
D001704:Biopolymers; D009841:Oligonucleotides; D013942:Thymine Nucleotides