Adenosine inhibits the release of interleukin-1beta in activated human peripheral mononuclear cells.
The effects of adenosine and subtype-specific activators of adenosine receptors (A1, A2A, A2B and A3) were studied on the release of interleukin-1beta (IL-1beta) from peripheral mononuclear cells, monocytes and lymphocytes. In the cells activated by the protein kinase C specific phorbol ester (phorbol 12-myristate 13-acetate) and Ca(2+) ionophore (A23187) both adenosine and the subtype-specific receptor agonists, CPA (A1), CGS 21680 (A2A) and IB-MECA (A3) induced a concentration-dependent inhibition of IL-1beta release. The rank order of potency in the inhibition of IL-1beta release was CPA=CGS 21680>IB-MECA>adenosine>NECA (in the presence of A1, A2A and A3 receptor inhibitors). The inhibitory actions of CPA, CGS 21680 or IB-MECA were significantly reduced in the presence of DPCPX, ZM 243185 or MRS 1191 as subtype-specific antagonists on A1, A2A and A3 adenosine receptors, respectively. It can be concluded that adenosine inhibits the release of IL-1beta from the activated human peripheral mononuclear cells. In this process A1, A2A and A3 receptors are involved.
Sipka
Sandor
S
3rd Department of Internal Medicine, University of Debrecen, Hungary. sipka@iiibel.dote.hu
Kovács
Ildikó
I
Szántó
Sándor
S
Szegedi
Gyula
G
Brugós
László
L
Bruckner
Geza
G
József Szentmiklósi
A
A
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology; D058906:Purinergic P1 Receptor Agonists; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007375:Interleukin-1; D010627:Phenethylamines; D058906:Purinergic P1 Receptor Agonists; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; D000001:Calcimycin; D000241:Adenosine; D013755:Tetradecanoylphorbol Acetate