Functional analysis of the pyrimidine de novo synthesis pathway in solanaceous species.
Pyrimidines are particularly important in dividing tissues as building blocks for nucleic acids, but they are equally important for many biochemical processes, including sucrose and cell wall polysaccharide metabolism. In recent years, the molecular organization of nucleotide biosynthesis in plants has been analyzed. Here, we present a functional analysis of the pyrimidine de novo synthesis pathway. Each step in the pathway was investigated using transgenic plants with reduced expression of the corresponding gene to identify controlling steps and gain insights into the phenotypic and metabolic consequences. Inhibition of expression of 80% based on steady-state mRNA level did not lead to visible phenotypes. Stepwise reduction of protein abundance of Asp transcarbamoylase or dihydro orotase resulted in a corresponding inhibition of growth. This was not accompanied by pleiotropic effects or by changes in the developmental program. A more detailed metabolite analysis revealed slightly different responses in roots and shoots of plants with decreased abundance of proteins involved in pyrimidine de novo synthesis. Whereas in leaves the nucleotide and amino acid levels were changed only in the very strong inhibited plants, the roots show a transient increase of these metabolites in intermediate plants followed by a decrease in the strong inhibited plants. Growth analysis revealed that elongation rates and number of organs per plant were reduced, without large changes in the average cell size. It is concluded that reduced pyrimidine de novo synthesis is compensated for by reduction in growth rates, and the remaining nucleotide pools are sufficient for running basic metabolic processes.
Schröder
Michael
M
Botanisches Institut, Im Neuenheimer Feld 360, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany.
Giermann
Norbert
N
Zrenner
Rita
R
D001221:Aspartate Carbamoyltransferase / Q000235:genetics / Q000378:metabolism; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics / Q000378:metabolism; D004080:Dihydroorotase / Q000235:genetics / Q000378:metabolism; D004081:Dihydroorotate Oxidase / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011743:Pyrimidines / Q000096:biosynthesis* / Q000737:chemistry; D011198:Solanum tuberosum / Q000201:enzymology*; D013045:Species Specificity; D014026:Nicotiana / Q000201:enzymology*
D009097:Multienzyme Complexes; D011743:Pyrimidines; C027157:uridine 5'-monophosphate synthase; D004081:Dihydroorotate Oxidase; D001221:Aspartate Carbamoyltransferase; D009962:Orotate Phosphoribosyltransferase; D004080:Dihydroorotase; D009964:Orotidine-5'-Phosphate Decarboxylase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)