[Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes].
Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants without hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.
Kishchenko
E M
EM
Komarnitskiĭ
I K
IK
Kuchuk
N V
NV
D027461:Beta vulgaris / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D018744:DNA, Plant / Q000032:analysis; D005966:Glucuronidase / Q000096:biosynthesis; D007150:Immunohistochemistry; D010940:Plant Proteins / Q000096:biosynthesis; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D027343:Rhizome / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D014170:Transformation, Genetic
D018744:DNA, Plant; D010940:Plant Proteins; D005966:Glucuronidase