Increase in inflammatory cytokines in median nerves in a rat model of repetitive motion injury.
We examined cytokines in rat median nerves following performance of a high repetition reaching and grasping task at a rate of 8 reaches/min for up to 8 weeks. IL-1alpha, IL-1beta, TNF-alpha, IL-6 and IL-10 were analyzed by immunohistochemistry. Double-labeling immunohistochemistry for ED1, a marker of phagocytic macrophages, was also performed. We found increased immunoexpression of IL-6 by week 3, increases in all 5 cytokines by week 5. This response was transient as all cytokines returned to control levels by 8 weeks of performance of a high repetition negligible force task. Cytokine sources included Schwann cells, fibroblasts and phagocytic macrophages (ED1-immunopositive). These findings suggest that cytokines are involved in the pathophysiology of repetitive motion injuries in peripheral nerves.
Al-Shatti
Talal
T
Kuwait University, Faculty of Allied Health Sciences, P.O. Box 31470, Sulaibekhat, Kuwait 90805, Kuwait. mary.barbe@temple.edu
Barr
Ann E
AE
Safadi
Fayez F
FF
Amin
Mamta
M
Barbe
Mary F
MF
D000704:Analysis of Variance; D000818:Animals; D012090:Cumulative Trauma Disorders / Q000150:complications / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D053331:Ectodysplasins; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation / Q000209:etiology / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008475:Median Nerve / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism; D013997:Time Factors
D016207:Cytokines; D053331:Ectodysplasins; D008565:Membrane Proteins