title: [Study on the reaction mechanism of chloroacetanilide herbicides with urease using fluorescence spectrum and high-performance liquid chromatography].
abstract: The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
authors: Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China.
authors: Hui-jun
authors: HJ
authors: Liu
authors: Xiu-Ming
authors: XM
authors: Zhan
authors: Ke-bin
authors: KB
authors: Li
authors: Wei-ping
authors: WP
authors: Liu
mesh_terms: D000081:Acetamides / Q000737:chemistry* / Q000378:metabolism; D000083:Acetanilides / Q000737:chemistry; D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D015394:Molecular Structure; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014052:Toluidines / Q000737:chemistry; D014510:Urease / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000081:Acetamides; D000083:Acetanilides; D014052:Toluidines; C118050:pretilachlor; C078188:2-chloro-N-(ethoxymethyl)-N-(2-methyl-6-(trifluoromethyl)phenyl)acetamide; C043377:acetochlor; C054409:butachlor; D014510:Urease; C051786:metolachlor