[Sequence and polymorphism analysis of porcine hormone-sensitive lipase gene 5'-UTR and exon I].
Hormone-sensitive lipase (HSL) is the key enzyme responsible for the mobilization of free acids from adipose tissue, and it is also the most important enzyme that affect fat deposition. In this paper, the porcine hormone-sensitive lipase gene 5'-UTR and exon I were sequenced. The sequence number in GenBank are AY332499, AY332497, AY332504, AY332505. A GC-CG in the DNA sequence -13 - -12 bp of porcine HSL gene 5'-UTR was detected between Duroc, Meishan, Qingping pig, Largewhite and Landrace. A G-->A missense mutation was detected in HSL gene exon I of different pig breeds. The characterization of the BsaH I PCR-RFLP polymorphism in exon I of the porcine HSL gene of different breeds and "Largewhite x Meishan" F2 group was analyzed. By association analysis between BsaH I PCR-RFLP polymorphism and GG, GG, AA genotypes of HSL gene exon I, a significant difference of pig eye area was found between AG and GG genotypes (P<0.05) in F2 group.
Lei
Ming-Gang
MG
College of Animal Science, Huazhong Agriculture University, Wuhan 430070, China. Leimg@mail.hzau.edu.cn
Wu
Zhen-Fang
ZF
Deng
Chang-Yan
CY
Dai
Li-He
LH
Zhang
Zhen-Bo
ZB
Xiong
Yuan-Zhu
YZ
D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D002787:Sterol Esterase / Q000235:genetics*; D013552:Swine / Q000145:classification / Q000235:genetics*
D004247:DNA; D002787:Sterol Esterase