Ferrite supports for isolation of DNA from complex samples and polymerase chain reaction amplification.
The influence of cobalt ferrite particles, with non-modified or modified surface, on the course of polymerase chain reaction (PCR) was investigated. DNA isolated from bacterial cells of Bifidobacterium bifidum was used in PCR evaluation of magnetic microspheres. The presence of cobalt ferrite particles inhibits PCR amplification. The effect is not dependent on the functional groups of the modifying reagents used (none, amino, carboxyl). Amplification was improved after the magnetic separation of magnetic particles. Proposed indirect method enabled verification of the suitability of designed particles for their application in PCR assays. Magnetic particles coated with alginic acid under high PEG and sodium chloride concentration were used for the isolation of PCR-ready bacterial DNA from various dairy products. DNA was isolated from crude bacterial cell lysates without phenol extraction of samples. Bifidobacterium and Lactobacillus DNAs were identified in dairy products using PCR.
Spanová
Alena
A
Masaryk University in Brno, Faculty of Science, CZ-602 00 Brno, Czech Republic
Rittich
Bohuslav
B
Benes
Milan J
MJ
Horák
Daniel
D
D001644:Bifidobacterium / Q000235:genetics; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D007778:Lactobacillus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D005290:Ferric Compounds; C001215:ferrite