Detection of betaig-H3, a TGFbeta induced gene, during cardiac development and its complementary pattern with periostin.
Regulation of normal cardiac development involves numerous transcription factors, cytoskeletal proteins, signaling molecules, and extracellular matrix proteins. These key molecular components act in concert to induce morphological changes essential for the proper development of a functional four-chambered heart. Growth factors such as BMPs and TGFbeta's play a role in migration, proliferation and differentiation during cardiac development and are important regulators of the extracellular matrix (ECM). Genes responsive to these morphogens are likely to play an equally significant role during cardiac development. Therefore, we sought to clone the chicken TGFbeta induced gene betaig-H3 and evaluate its spatio-temporal expression during heart morphogenesis. Our studies show by Northern analysis, whole mount and section in situ hybridization experiments that betaig-H3 is expressed primarily in the mesenchyme of the atrioventricular and outflow tract cushions and later in the right and left atrioventricular valve leaflets and supporting valve structures. The mRNA expression domains of betaig-H3 show a complementary pattern compared to that of its highly homologous relative, periostin.
Norris
Russell A
RA
Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Ave BSB 642, Charleston, SC 29425, USA.
Kern
Christine B
CB
Wessels
Andy
A
Wirrig
Elaine E
EE
Markwald
Roger R
RR
Mjaatvedt
Corey H
CH
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002642:Chick Embryo; D017124:Conserved Sequence; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006321:Heart / Q000196:embryology*; D006352:Heart Ventricles / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D008943:Mitral Valve / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D014261:Tricuspid Valve / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D000099222:betaIG-H3 Protein
D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C512835:POSTN protein, human