Inhibitory activity on matrix metalloproteinases and cell-proliferating activity of tissue inhibitor of metalloproteinases-1 (TIMP-1)-contrastive difference between human and bovine TIMP-1s on mouse cell proliferation.
Either human or bovine TIMP-1 inhibited matrix metalloproteinase (MMP) activities, such as collagenolytic, gelatinolytic and caseinolytic, expressed by mouse cells as well as those expressed by human cells. Bovine TIMP-1 stimulated the proliferation of both human and mouse cells, but human TIMP-1 only proliferate human cells and not mouse cells indicating that the cell-proliferating activity has more strict species specificity than MMP inhibitory activity. All the results from [(3)H]thymidine incorporation, the phosphorylation of tyrosine-containing cellular proteins and extracellular-signal-regulated protein kinases supported the cell-proliferating properties of both human and bovine TIMP-1s. Human TIMP-1 seems not to bind to TIMP-1 receptors on mouse cells.
Nishimura
Kazuaki
K
Department of Orthodontics, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
Yamashita
Kyoko
K
Kato
Yuki
Y
Iwata
Toshio
T
Iwata
Kazushi
K
Nishikawa
Taira
T
Goto
Shigemi
S
Hayakawa
Taro
T
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured / Q000378:metabolism; D006224:Cricetinae; D017077:Culture Media, Conditioned / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000737:chemistry; D013045:Species Specificity; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D061965:Matrix Metalloproteinase Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D019000:Phosphotyrosine; D048049:Extracellular Signal-Regulated MAP Kinases