title: High levels of nucleotide diversity in the European rabbit (Oryctolagus cuniculus) SRY gene.
abstract: We have sequenced 2,388 bp of the European rabbit sex determining region Y (SRY) gene. These data provide a 10-fold increase in the coverage of the Y chromosome in this species, including the entire open reading frame of the SRY, the polyadenylation signal, and two repetitive sequences in the 5' -region. A survey of 2021 bp of this gene in eight domestic breeds and four wild individuals revealed a total of nine single nucleotide polymorphisms and one indel, defining two deeply divergent lineages. The resulting estimation of nucleotide diversity (pi=1.34 x10(-3)) is very high when compared with other species, but no variability was detected among the domestic breeds. This study represents a first step in the characterization of the European rabbit Y chromosome and its variability. These sequences can be used in additional phylogeographical analyses of the European rabbit and other Leporid species, as well as in evolutionary studies of sex determination and the Y chromosome in wild species.
authors: CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, and Departamento de Zoologia e Antropologia, Faculdade de Ciências da Universidade do Porto, Portugal. ageraldes@mail.icav.up.pt
authors: A
authors: A
authors: Geraldes
authors: C
authors: C
authors: Rogel-Gaillard
authors: N
authors: N
authors: Ferrand
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005060:Europe; D040461:Gene Components; D024282:Genes, sry / Q000235:genetics*; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011817:Rabbits / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers