Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate.
Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of more than one gene cannot be monitored simultaneously by this system because one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay system in which three luciferases that emit green, orange, and red light with a single substrate are used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply and rapidly monitor multiple gene expressions in a one-step reaction.
Nakajima
Yoshihiro
Y
National Institute of Advanced Industrial Science and Technology, Osaka, Japan.
Kimura
Takuma
T
Sugata
Kazunori
K
Enomoto
Toshiteru
T
Asakawa
Atsushi
A
Kubota
Hidehiro
H
Ikeda
Masaaki
M
Ohmiya
Yoshihiro
Y
D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids; D013379:Substrate Specificity
D008156:Luciferases