title: Does apoptosis occur in amyotrophic lateral sclerosis? TUNEL experience from human amyotrophic lateral sclerosis (ALS) tissues.
abstract: The role that apoptosis plays in the pathogenesis of amyotrophic lateral sclerosis (ALS) is still unclear. From our autopsy samples, we have undertaken an effort to verify if apoptosis in ALS really occurs or if can at least be detected. The study was performed using TUNEL method for screening the apoptotic changes in the autopsy samples from 8 ALS cases compared with 16 control cases. No features of apoptosis (DNA cleavages) were noted in any of the investigated regions of the central nervous system in ALS cases as well as in controls. These preliminary results seem to support the reports, which deny the role of apoptosis in human ALS. The following investigations using additional methods will be performed for detection the apoptotic signals in ALS.
authors: Department of Neurology, Jagiellonian University Medical College, Botaniczna 3, 31-503 Krakow, Poland. tomik@neuro.cm-uj.krakow.pl
authors: Barbara
authors: B
authors: Tomik
authors: Dariusz
authors: D
authors: Adamek
authors: Piotr
authors: P
authors: Pierzchalski
authors: Steven
authors: S
authors: Banares
authors: Aleksandra
authors: A
authors: Duda
authors: Dorota
authors: D
authors: Partyka
authors: Wiesław
authors: W
authors: Pawlik
authors: Józef
authors: J
authors: Kałuza
authors: Stan
authors: S
authors: Krajewski
authors: Andrzej
authors: A
authors: Szczudlik
mesh_terms: D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology*; D017209:Apoptosis / Q000502:physiology*; D001921:Brain / Q000473:pathology*; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D009046:Motor Neurons / Q000473:pathology