title: Increased sensitivity to sparsely ionizing radiation due to excessive base excision in clustered DNA damage sites in Escherichia coli.
abstract: OBJECTIVE
  In order to clarify the cellular processing and repair mechanisms for radiation-induced clustered DNA damage, we examined the correlation between the levels of DNA glycosylases and the sensitivity to ionizing radiation in Escherichia coli.
  
  
  METHODS
  The lethal effects of gamma-rays, X-rays, alpha-particles and H2O2 were determined in E. coli with different levels of DNA glycosylases. The formation of double-strand breaks by post-irradiation treatment with DNA glycosylase was assayed with gamma-irradiated plasmid DNA in vitro.
  
  
  RESULTS
  An E. coli mutM nth nei triple mutant was less sensitive to the lethal effect of sparsely ionizing radiation (gamma-rays and X-rays) than the wild-type strain. Overproduction of MutM (8-oxoguanine-DNA glycosylase), Nth (endonuclease III) and Nei (endonulease VIII) increased the sensitivity to gamma-rays, whereas it did not affect the sensitivity to alpha-particles. Increased sensitivity to gamma-rays also occurred in E. coli overproducing human 8-oxoguanine-DNA glycosylase (hOgg1). Treatment of gamma-irradiated plasmid DNA with purified MutM converted the covalently closed circular to the linear form of the DNA. On the other hand, overproduction of MutM conferred resistance to H2O2 on the E. coli mutM nth nei mutant.
  
  
  CONCLUSIONS
  The levels of DNA glycosylases affect the sensitivity of E. coli to gamma-rays and X-rays. Excessive excision by DNA glycosylases converts nearly opposite base damage in clustered DNA damage to double-strand breaks, which are potentially lethal.
authors: Laboratory of Radiation Biology, Graduate School of Science, Kyoto University, Japan.
authors: P W
authors: PW
authors: Chang
authors: Q M
authors: QM
authors: Zhang
authors: K
authors: K
authors: Takatori
authors: A
authors: A
authors: Tachibana
authors: S
authors: S
authors: Yonei
mesh_terms: D004249:DNA Damage / Q000502:physiology*; D004252:DNA Mutational Analysis; D004260:DNA Repair / Q000502:physiology* / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial