title: In vitro evaluation of caries inhibition promoted by self-etching adhesive systems containing antibacterial agents.
abstract: This study evaluated the cariostatic effect of antibacterial self-etching adhesive systems, by means of an in vitro bacterial caries model. Seventy-five prepared bovine slabs were randomly divided into groups (n=15): (1) unbonded composite, no carious challenge (UNB-NC); (2) unbonded composite, carious challenge (UNB-C); (3) Clearfil SE Bond, no antibacterial agent (CSE); (4) Protect Bond, containing MDPB and fluoride (PB); and (5) Reactmer Bond, fluoride-releasing (RB). All preparations were restored with Filtek Z-250. Groups (2)-(5) were submitted to a medium containing Streptococcus mutans (ATCC-- 25175) for 5 days, and Group (1) was kept in a noninoculated medium. Insoluble polysaccharides present in tooth biofilms were quantified, Knoop hardness (KHN) was measured on the enamel adjacent to restorations, and standard 35-mm polarized light photomicrographs were taken as illustrations. Polysaccharide and Knoop hardness results were analyzed with the use of ANOVA, with a split-split-plot statistical design for KHN. Except for Group (1), all groups showed similar caries formation. Biofilm over PB restorations showed the smallest amounts of polysaccharides (14.37 microg/mg), and CSE showed the highest amounts (20.87 microg/mg). All self-etching systems tested were unable to inhibit secondary caries in a bacterial model simulating a high caries challenge, even though there was reduced glucan synthesis provided by the adhesive system containing MDPB and fluoride.
authors: Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, Avenida Limeira, 901 Piracicaba, São Paulo, 13414-903, Brazil.
authors: Maristela M
authors: MM
authors: Lobo
authors: Reginaldo B
authors: RB
authors: Gonçalves
authors: Luiz André F
authors: LA
authors: Pimenta
authors: Ana Karina B
authors: AK
authors: Bedran-Russo
authors: Patrícia N R
authors: PN
authors: Pereira
mesh_terms: D000269:Adhesives / Q000737:chemistry / Q000494:pharmacology; D000468:Alkalies / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D005459:Fluorides / Q000008:administration & dosage / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry; D012995:Solubility; D013295:Streptococcus mutans / Q000187:drug effects / Q000502:physiology; D014070:Tooth / Q000187:drug effects / Q000382:microbiology
chemical_list: D000269:Adhesives; D000468:Alkalies; D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D005459:Fluorides