title: Hypercholesterolemia attenuates postischemic ventricular dysfunction in the isolated rabbit heart.
abstract: The effects of the chronic administration of cholesterol on the stunned myocardium have not been studied. The objective was to determine the effect of a cholesterol enriched diet on postischemic ventricular dysfunction. In group 1 (G1, n = 7) isolated rabbit hearts underwent a follow up of ventricular function during 30 min in aerobic conditions. In group 2 (G2, n = 6) G1 was repeated but the animals were subjected to a 1% cholesterol enriched diet during 4 weeks (hypercholesterolemic animals). In group 3 (G3, n = 8) hearts underwent 15 min of global ischemia followed by 30 min of reperfusion. In Group 4 (G4, n = 11) G3 was repeated, but in hypercholesterolemic animals. Since cholesterol decreased the inotropism in basal situation, and this makes the comparison between groups difficult, we performed a Group 5 (G5, n = 7), in which G4 protocol was repeated but isoproterenol (8 microg/kg/min) was administered 10 min before ischemia, in order to match the preischemic inotropic state with respect to the normocholesterolemic ones. G1 and G2 maintained a stable inotropism during the 30 min of perfusion. The preischemic left ventricular developed pressure (LVDP) in G3 and G4 was 91.4 +/- 4.3 and 70.8 +/- 3.4 mmHg (p < 0.05), respectively, and after 30 min of reperfusion differences were not observed between G3 and G4. Nevertheless, when LVDP is expressed as a percentage, we detected an attenuation of postischemic systolic alterations in hypercholesterolemic animals (67.3 +/- 3.6 in G4 vs. 90.8 +/- 3.1% in G3, p < 0.05). When LVDP in G5 was increased until matching the one of G3, there were no differences after 30 min of reperfusion. Left ventricular end diastolic pressure increased 285 +/- 46%, 61 +/- 25% (p < 0.05 vs. G3 and G5) and 216 +/- 25% in G3, G4 and G5 at 30 min of reperfusion. There were no differences either in the values of tau or infarct size between groups. Thus, in hypercholesterolemic animals, a decrease of the preischemic inotropism exists and there is an attenuation of the stunned myocardium. When contractility of the normo and hypercholesterolemic animals is matched, the beneficial effect disappears.
authors: Institute of Cardiovascular Pathophysiology, Department of Pathology, Faculty of Medicine, University of Buenos Aires, Argentina.
authors: Verónica
authors: V
authors: D'Annunzio
authors: Martín
authors: M
authors: Donato
authors: Melina
authors: M
authors: Sabán
authors: Silvia M
authors: SM
authors: Sanguinetti
authors: Regina L W
authors: RL
authors: Wikinski
authors: Ricardo J
authors: RJ
authors: Gelpi
mesh_terms: D000818:Animals; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D006937:Hypercholesterolemia / Q000209:etiology*; D009200:Myocardial Contraction / Q000502:physiology; D017202:Myocardial Ischemia / Q000209:etiology / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000503:physiopathology*; D017682:Myocardial Stunning / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits; D018487:Ventricular Dysfunction, Left / Q000209:etiology / Q000503:physiopathology*
chemical_list: D002791:Cholesterol, Dietary