[Influence of the mutants of hepatitis B surface antigen on the cell immunity].
OBJECTIVE
To study the influence of the mutants of hepatitis B surface antigen on the cell immunity.


METHODS
The recombinant plasmids of NS2Swt, NS2S126, NS2S133, NS2S141 and NS2S145 were transfected into Chinese hamster ovary (CHO) cells and the expressed proteins were detected by means of ELISA. Following PHA-activated lymphoblasts proliferation assay and IFN-gamma, IL-2, IL-10 induction assay were done with these proteins.


RESULTS
It was identified that these proteins of HBsAg could stimulate human lymphoblasts proliferation. Besides, there were no significant difference between the mutants and the wild. It was deserved to point out that the HBsAg with T126S mutation could increase the expression of IFN-gamma in the culture medium while the HBsAg with M133T mutation induced more expression of IL-10.


CONCLUSIONS
The results suggested that the cellular immune response to mutants of HBV might not be strengthened or weakened. But it should not be ignored that HBV T126S or M133T mutation may assert a potential impact on the cell immunity.
Bai
Yu
Y
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Xia
Guo-liang
GL
Guo
Yu
Y
Cong
Yu
Y
Jia
Zhi-yuan
ZY
Guo
Min-zhuo
MZ
Zhao
Hong-lan
HL
Zhan
Mei-yun
MY
D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics*; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D014162:Transfection
D006514:Hepatitis B Surface Antigens; D007376:Interleukin-2; D050505:Mutant Proteins; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma