title: Danshen inhibits oxysterol-induced endothelial cell apoptosis in vivo.
abstract: Oxysterols induce apoptosis in vascular endothelial cells in vitro, but it is not clear whether they do so in vivo. We intravenously injected an oxysterol, cholestan-3beta, 5alpha, 6beta-triol, into rats and quantitatively analyzed endothelial cell apoptosis in the aorta. Oxysterol significantly promoted apoptosis in a time- and dose-dependent fashion. The apoptosis had increased 4.5-fold 6 hrs after injection, and returned to the background level at 48 hrs. An extract of the Chinese herb Danshen as well as probucol abolished triol-induced endothelial cell apoptosis in vitro and in vivo. Since apoptotic cells are quickly cleared, oxysterol-induced apoptosis could significantly affect endothelial integrity over a long period of time. Radical scavengers may be useful for the prevention of endothelial damage.
authors: National Cardiovascular Center Research Institute, Osaka, Japan.
authors: Toru
authors: T
authors: Nakazawa
authors: Ning
authors: N
authors: Xui
authors: Zeng
authors: Z
authors: Hesong
authors: Mikio
authors: M
authors: Kinoshita
authors: Tsuyoshi
authors: T
authors: Chiba
authors: Eiji
authors: E
authors: Kaneko
authors: Katsumasa
authors: K
authors: Yui
authors: Kentaro
authors: K
authors: Shimokado
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects*; D018929:Cell Culture Techniques; D002777:Cholestanols / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D000960:Hypolipidemic Agents / Q000008:administration & dosage*; D007275:Injections, Intravenous; D008297:Male; D010618:Phenanthrolines / Q000008:administration & dosage*; D011341:Probucol / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
chemical_list: D000975:Antioxidants; D002777:Cholestanols; D004365:Drugs, Chinese Herbal; D000960:Hypolipidemic Agents; D010618:Phenanthrolines; C000474:cholestane-3,5,6-triol; C028972:dan-shen root extract; D011341:Probucol