HCV-RNA qualitative assay based on transcription mediated amplification improves the detection of hepatitis C virus infection in patients on hemodialysis: results from five hemodialysis units in central Greece.
BACKGROUND
End-stage renal disease patients (ESRD) on maintenance hemodialysis (HD) are at increased risk of acquiring hepatitis C virus (HCV) infection. An early and accurate diagnosis of HCV infection is important for the prevention of viral transmission and the management of ESRD patients on HD but conventional ELISA and PCR have often failed to reveal active HCV infection.


OBJECTIVE
This study evaluated the prevalence of HCV infection in ESRD patients from all HD units in central Greece using a sensitive HCV-RNA transcription mediated amplification (TMA) assay and compared its sensitivity with that of anti-HCV ELISA.


METHODS
Anti-HCV antibody (third generation ELISA), HCV-RNA (TMA) and HCV genotypes (HCV TMA-LiPA) were determined in 366 ESRD Greek patients.


RESULTS
In total, 132 (36%) ESRD patients were HCV positive by ELISA or TMA; 44 by TMA alone, 16 by ELISA alone and 72 positive by both assays. More than half of the viraemic patients had genotype 3a.


CONCLUSIONS
HCV-RNA (TMA) assay appears to increase the accuracy in the diagnosis of HCV infection in HD patients compared to the anti-HCV ELISA and could serve as an additional screening tool in these patients.
Rigopoulou
Eirini I
EI
Faculty of Health Sciences, Department of Medicine, Academic Liver Unit and Research Laboratory of Internal Medicine, Medical School, University of Thessaly, 22 Papakiriazi str., 412 22 Larissa, Greece.
Stefanidis
Ioannis
I
Liaskos
Christos
C
Zervou
Eleftheria K
EK
Rizos
Christos
C
Mina
Paraskevi
P
Zachou
Kalliopi
K
Syrganis
Christos
C
Patsidis
Eftichios
E
Kyriakopoulos
George
G
Sdrakas
Lambros
L
Tsianas
Nikolaos
N
Dalekos
Georgios N
GN
D005260:Female; D005784:Gene Amplification; D006115:Greece / Q000453:epidemiology; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology* / Q000209:etiology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000628:therapy; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006435:Renal Dialysis / Q000009:adverse effects*; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D012367:RNA, Viral