title: Blood transfusion and iatrogenic risks in Mexico City. Anti-Trypanosoma cruzi seroprevalence in 43,048 blood donors, evaluation of parasitemia, and electrocardiogram findings in seropositive.
abstract: Iatrogenous transmission of Trypanosoma cruzi by blood transfusion was suggested as a potential risk by Pellegrino (1949). Seropositive blood donors in Mexico were first reported in 1978, however, limited information is available due to small sampling, the use of heterogeneous serologic assays, and geographically limited studies. A wide survey carried out in 18 out of the 32 states of Mexico, showed a national mean of 1.6% seropositive among 64,969 donors, ranging from 0.2 to 2.8%. In the present study, we have screened 43,048 voluntary blood donors in a period of five years at the Instituto Nacional de Cardiología I. Chávez, a concentration hospital located in Mexico city which serves mainly the metropolitan area and accepts from all over the country. Standardized ELISA and IIF were used to identify seropositive individuals in addition to hemoculture, PCR and standard 12 lead ECG tests that were applied to a group of seropositive patients (29/161). The result showed a seropositivity of 0.37% (161/43,048). From the group of seropositive individuals 40% (12/29) were potential carriers of T. cruzi at the donation time and 5/29 had subclinical ECG abnormalities. Parasitological tests performed in 70 erythrocyte and platelet fractions from seropositive units (70/161) showed negative results. Our findings strongly support T. cruzi screening in the transfusion medicine practice and identify subclinical heart disease among seropositive blood donors.
authors: Laboratorio Inmunoparasitología, Instituto Nacional Cardiología I. Chávez, Juan Badiano 1, Taplan, 14080 México DF.
authors: Nidia
authors: N
authors: Hernández-Becerril
authors: Ana Maria
authors: AM
authors: Mejía
authors: Martha Alicia
authors: MA
authors: Ballinas-Verdugo
authors: Verónica
authors: V
authors: Garza-Murillo
authors: Elsa
authors: E
authors: Manilla-Toquero
authors: Ruth
authors: R
authors: López
authors: Sergio
authors: S
authors: Trevethan
authors: Manuel
authors: M
authors: Cardenas
authors: Pedro Antonio
authors: PA
authors: Reyes
authors: Kenji
authors: K
authors: Hirayama
authors: Victor Manuel
authors: VM
authors: Monteón
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001782:Blood Donors; D014355:Chagas Disease / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission; D004562:Electrocardiography; D005260:Female; D006801:Humans; D008297:Male; D008403:Mass Screening; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D018512:Parasitemia / Q000097:blood; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D011795:Surveys and Questionnaires; D065227:Transfusion Reaction; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan