[Effects of different factors on direct induction of microtubers from explants in Pinellia ternate].
OBJECTIVE
To study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose.


METHODS
Using the orthogonal design method.


CONCLUSIONS
The optimal media to directly induce microtubers from leaves were MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3% and MS+ 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + sucrose 3%. Optimal media to directly induce microtubers from tubers were MS + 6-BA 1.0 mg x L(-1) + PEG 5% + sucrose 5% and MS+ CH 500 mg x L(-1) + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + AC 0.5% + sucrose 5%. Media suitable for plantlet growth were MS + 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + AC 0.5% + sucrose 5% and MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3%.
Zhang
Ai-Min
AM
Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
Yang
Sheng-Yu
SY
Xue
Jian-Ping
JP
Sheng
Wei
W
Gao
Xiang
X
Zhu
Yan-Fang
YF
D002244:Carbon / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005947:Glucose / Q000494:pharmacology; D030021:Pinellia / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000254:growth & development; D035281:Plant Tubers / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
D002364:Caseins; D003470:Culture Media; D010937:Plant Growth Regulators; D011092:Polyethylene Glycols; D013395:Sucrose; C475729:casein hydrolysate; D002244:Carbon; D005947:Glucose