[Chemically modified specific immunoglobulins for legionellosis laboratory diagnostics].
On an example of the rabbit antilegionella immunoglobulins (antLegIgRb) and monospecific human serum (antLegSerHum) the influence of a protein with different acylation level on specific and nonspecific activity of the above immunobiopreparations was investigated. It was determined, that 5 % level of chemical modification provides the highest level of augmentation (2.0-2.7 times) of specific and nonspecific activity of antLegIgRb and antLegSerHum. Acylated forms of immunoglobulins and sera retain the beneficial biological properties (high level of specific activity, ability to be labelled with fluorochromes, resistance at long-term storage) and can be used for producing diagnostic immunobiological drugs.
Martynov
A V
AV
Tymchenko
O M
OM
Pokhyl
S I
SI
D000215:Acylation; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D007875:Legionella / Q000276:immunology*; D007876:Legionellosis / Q000175:diagnosis* / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity
D000942:Antigens, Bacterial; D007136:Immunoglobulins