title: Enantioselective epoxidation of linolenic acid catalysed by cytochrome P450(BM3) from Bacillus megaterium.
abstract: Cytochrome P450(BM3), from Bacillus megaterium, catalyses the epoxidation of linolenic acid yielding 15,16-epoxyoctadeca-9,12-dienoic acid with complete regio- and moderate enantio-selectivity (60% ee). The absolute configuration of the product is tentatively assigned as 15(R),16(S)-. The Michaelis-Menten parameters kcat and Km for the reaction were determined to be 3126 +/- 226 min(-1) and 24 +/- 6 microM respectively.
authors: School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, UK EH9 3JJ.
authors: Ayhan
authors: A
authors: Celik
authors: Davide
authors: D
authors: Sperandio
authors: Robert E
authors: RE
authors: Speight
authors: Nicholas J
authors: NJ
authors: Turner
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D013237:Stereoisomerism; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: D004852:Epoxy Compounds; D017962:alpha-Linolenic Acid; D003577:Cytochrome P-450 Enzyme System