Heterotopic transplantation of glycerin-preserved trachea: effect of respiratory epithelium desquamation on acute rejection.
An effective preservation method and decreased rejection are essential for tracheal transplantation in the reconstruction of large airway defects. Our objective in the present study was to evaluate the antigenic properties of glycerin-preserved tracheal segments. Sixty-one tracheal segments (2.4 to 3.1 cm) were divided into three groups: autograft (N = 21), fresh allograft (N = 18) and glycerin-preserved allograft (N = 22). Two segments from different groups were implanted into the greater omentum of dogs (N = 31). After 28 days, the segments were harvested and analyzed for mononuclear infiltration score and for the presence of respiratory epithelium. The fresh allograft group presented the highest score for mononuclear infiltration (1.78 +/- 0.43, P < or = 0.001) when compared to the autograft and glycerin-preserved allograft groups. In contrast to the regenerated epithelium observed in autograft segments, all fresh allografts and glycerin-preserved allografts had desquamation of the respiratory mucosa. The low antigenicity observed in glycerin segments was probably the result of denudation of the respiratory epithelium and perhaps due to the decrease of major histocompatibility complex class II antigens.
Saueressig
M G
MG
Serviço de Cirurgia Torácica, Hospital de Clínicas de Porto Alegre, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. msaueressig@hcpa.ufrgs.br
Edelweiss
M I A
MI
Souza
F H
FH
Moreschi
A H
AH
Savegnago
F L
FL
Macedo Neto
A V
AV
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents; D004285:Dogs; D005260:Female; D005990:Glycerol; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D009852:Omentum / Q000601:surgery; D009926:Organ Preservation / Q000379:methods; D020545:Respiratory Mucosa / Q000276:immunology* / Q000473:pathology; D014132:Trachea / Q000276:immunology / Q000473:pathology / Q000637:transplantation*; D016042:Transplantation, Heterotopic / Q000276:immunology* / Q000473:pathology
D003451:Cryoprotective Agents; D005990:Glycerol