Isolation and characterization of a new Mycobacterium austroafricanum strain, IFP 2015, growing on MTBE.
A new Mycobacterium austroafricanum strain, IFP 2015, growing on methyl tert-butyl ether (MTBE) as a sole carbon source was isolated from an MTBE-degrading microcosm inoculated with drain water of an MTBE-supplemented gasoline storage tank. M. austroafricanum IFP 2015 was able to grow on tert-butyl formate, tert-butyl alcohol (TBA) and alpha-hydroxyisobutyrate. 2-Methyl-1,2-propanediol was identified as the TBA oxidation product in M. austroafricanum IFP 2015 and in the previously isolated M. austroafricanum IFP 2012. M. austroafricanum IFP 2015 also degraded ethyl tert-butyl ether more rapidly than M. austroafricanum IFP 2012. Specific primers designed to monitor the presence of M. austroafricanum strains could be used as molecular tools to detect similar strains in MTBE-contaminated environment.
Lopes Ferreira
Nicolas
N
Institut Français du Pétrole, 1-4, avenue de Bois-Préau, 92852, Rueil-Malmaison, France.
Maciel
Helena
H
Mathis
Hugues
H
Monot
Frédéric
F
Fayolle-Guichard
Françoise
F
Greer
Charles W
CW
D001426:Bacterial Proteins / Q000235:genetics; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D005742:Gasoline; D008738:Methyl Ethers / Q000378:metabolism*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001426:Bacterial Proteins; D018834:Chaperonin 60; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005742:Gasoline; D008738:Methyl Ethers; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C074119:heat-shock protein 65, Mycobacterium; C043243:methyl tert-butyl ether; D018833:Chaperonins