Peptic digestibility of raw and heat-coagulated hen's egg white proteins at acidic pH range.
Allergenicity in food proteins is generally dependent on their heat stability and resistance to digestive enzymes together with the presence of IgE-recognizing epitopes on the molecules. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, we assessed peptic digestibility of raw and heat-coagulated hen's egg white proteins at acidic pH range (1.5-4.0). Ovalbumin in raw egg white was slightly digested by pepsin at pH 1.5 and pH 2.0, and was almost resistant to the enzyme at pH 2.5 and over, which was altered in heat-coagulated egg white at the pH range from 1.5 to 2.5 where the protein was well digestive against the enzyme. Peptic digestibility of ovomucoid in raw egg white was good at the pH range from 1.5 to 2.5, but almost non-existent at pH 3.0 and over where the improvement of the digestibility of the protein was not found even in heat-coagulated egg white. As the stomach in new born infants shows a low amount of secretary pepsin and an out of optimum pH of peptic activity, low digestibility of ovalbumin and ovomucoid in raw and heat-coagulated egg white at over pH 3.0 is supposed to be responsible for their allergenicity and delayed outgrowth from hen's egg allergy in patients with delayed maturation of stomach functions.
Yoshino
Kenji
K
Department of Nutrition, School of Medicine, The University of Tokushima, Japan.
Sakai
Kentaro
K
Mizuha
Yoko
Y
Shimizuike
Ayako
A
Yamamoto
Shigeru
S
D000818:Animals; D004063:Digestion / Q000502:physiology*; D021181:Egg Hypersensitivity / Q000209:etiology / Q000378:metabolism; D015387:Egg Proteins, Dietary / Q000378:metabolism*; D004529:Egg White; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D010047:Ovalbumin / Q000378:metabolism; D010059:Ovomucin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism*
D015387:Egg Proteins, Dietary; D010059:Ovomucin; D010047:Ovalbumin; D010434:Pepsin A