Determination of saikosaponins a, c, and d in Bupleurum Chinese DC from different areas by capillary zone electrophoresis.
A fast capillary zone electrophoresis (CZE) method was developed for the determination and separation of saikosaponins a, c, and d in Chinese herbal extracts of Bupleurum Chinese DC from different areas. Detection at 214 nm with a system containing sodium borate buffer and mono-3-phenylcarbamoyl-beta-CD was found to be the most suitable approach for this analysis. Saikosaponins a, c, and d could be easily determined within 8 min. The effect of the concentration of mono-3-phenylcarbamoyl-beta-CD, the concentration of the running buffer and buffer pH value on the migration behavior of the saikosaponins is discussed.
Lin
Xiuli
X
School of Pharmacy, Shandong University, Wen Hua Xi Road 44, Jinan 250012, P. R. China.
Xue
Ling
L
Zhang
Huiyun
H
Zhu
Chenfu
C
D001881:Borates / Q000737:chemistry; D002021:Buffers; D029972:Bupleurum / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D019075:Electrophoresis, Capillary; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D011208:Powders; D015203:Reproducibility of Results; D012503:Saponins / Q000302:isolation & purification*; D013237:Stereoisomerism
D001881:Borates; D002021:Buffers; D003505:Cyclodextrins; D004365:Drugs, Chinese Herbal; D011208:Powders; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D