title: Confirmation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 in shellfish as their anthrylmethyl derivatives using UV radiation.
abstract: A rapid and simple method for confirmation of the diarrhetic shellfish poisons (DSP): okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) using fluorescence detection following derivatization with 9-chloromethylanthracene, has been established as an alternate to LC/MS. Exposure of the anthrylmethyl derivatives of OA, DTX-1 and DTX-2 to near UV light (300-400 nm) resulted in the loss of these compounds to below detection limits within 30 min, with a concurrent appearance of two additional compounds. Based on the mass spectral evidence, we propose that these newly formed compounds are the decarboxylation products of the derivatized diarrhetic shellfish poisons. UV radiation is, therefore, proposed as a rapid and simple confirmation technique for these DSP in mussel samples.
authors: Food Research Division, Bureau of Chemical Safety, Health Products and Food Branch, Health Canada, Sir Frederick Banting Research Centre, 2203D, Tunney's Pasture, Ottawa, Ont., Canada K1A 0L2. thea_rawn@hc-sc.gc.ca
authors: Dorothea F K
authors: DF
authors: Rawn
authors: Cathie
authors: C
authors: Ménard
authors: Barbara
authors: B
authors: Niedzwiadek
authors: David
authors: D
authors: Lewis
authors: Benjamin P Y
authors: BP
authors: Lau
authors: Nathalie
authors: N
authors: Delauney-Bertoncini
authors: Marie Claire
authors: MC
authors: Hennion
authors: James F
authors: JF
authors: Lawrence
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000737:chemistry; D049872:Bivalvia / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D004141:Dinoflagellida / Q000737:chemistry; D008387:Marine Toxins / Q000032:analysis* / Q000528:radiation effects; D013058:Mass Spectrometry / Q000379:methods; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000528:radiation effects; D011714:Pyrans / Q000032:analysis* / Q000528:radiation effects; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D000873:Anthracenes; D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C099162:9-chloromethylanthracene; C051904:dinophysistoxin 1; C076221:dinophysistoxin 2