title: Wavelength-scanning surface plasmon resonance imaging.
abstract: A new surface plasmon resonance (SPR) imaging technique was proposed. After measurements were conducted at varying wavelengths, the wavelength affording the minimum brightness (SPR wavelength) was determined at each pixel of the image. A two-dimensional map of the SPR wavelength could be converted to a thickness profile by use of a nonlinear calibration curve, which was obtained by Fresnel calculation. An array of protein thin layers on a gold film was evaluated in air to present the layers' surface structure in nanometer scale.
authors: Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology, Hayashi-cho, Takamatsu 761-0395, Japan. otsuki-s@aist.go.jp
authors: Soichi
authors: S
authors: Otsuki
authors: Kaoru
authors: K
authors: Tamada
authors: S
authors: S
authors: Wakida
mesh_terms: D000465:Algorithms; D002138:Calibration; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*