[Differentiation of mouse bone marrow mesenchymal stem cells into hepatocyte in vivo].
To explore the possibility of mouse bone marrow mesenchymal stem cells (BMSCs) differentiating into hepatocyte in vivo, the BMSCs isolated from donor male BALB/C mice at age of 6-8 weeks were transplanted to female BALB/C mice as recipients by transfusion via tail vein or direct liver injection. By the end of 1st, 2nd, 4th weeks after transplantation, the Y chromosome positive and albumin expression positive hepatocytes were detected from the recipient female mouse liver tissue by fluorescence in situ hybridization (FISH ) and immunohistochemistry (IHC). In the two groups of direct liver injection, no matter whether the donor cells are fresh isolated bane marrow cells or passage 3 cells, the Y chromosome positive cells with albumin expression simultaneously were detected by the end of the 1st week after transplantation. Similar result has been observed in the other subgroup of tail vein injection, but only by the end of the 2nd week after transplantation. BMSCs are capable of differentiating into hepatocyte in vivo.
Zhang
Yi
Y
Key Laboratory for Biotherapy of Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu 610041, China.
Fan
Yulana
Y
Zhao
Liansan
L
Tang
Hong
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D005260:Female; D022781:Hepatocytes / Q000166:cytology*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C