Macrophages promote development of human erythroid precursors in transferrin-free culture medium.
Iron is mandatory for cell growth and development. Erythroid precursors need iron to a greater degree for hemoglobinization. Culturing erythroid precursors under serum and transferrin-free conditions resulted in their death, whereas under the same conditions, but in the presence of macrophages erythroid cell growth and development was evident as measured by hemoglobin (Hb)-specific cytochemical staining, flow cytometric immuno-staining of glycophorin A and Hb quantitation by a spectrophotometric method as well as by high performance liquid chromatography. Thus, macrophages support erythroid cell growth in the absence of transferrin, most likely by providing iron.
Leimberg
J M
JM
Department of Human Nutrition and Metabolism, Faculty of Medicine, The Hebrew University, Jerusalem, Israel.
Konijn
A M
AM
Fibach
E
E
D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D014168:Transferrin
D003470:Culture Media; D014168:Transferrin