Protective effects of a DNA vaccine expressing the infectious salmon anemia virus hemagglutinin-esterase in Atlantic salmon.
Infectious salmon anemia (ISA) is a disease, caused by an orthomyxovirus, which has considerable economic impact on farming of Atlantic salmon. Here we describe the results of immunization against ISA using plasmids expressing the ISA virus hemagglutinin-esterase (HE). Immunized Atlantic salmon demonstrated moderate protection after challenge with ISA virus, with relative percent survival of 39.5 and 60.5 in two parallel groups. No protection was seen after immunization using a plasmid expressing the ISA virus nucleoprotein. Fish in the HE-immunized group had earlier onset of clearance of the virus than control fish. There was no detectable ISA virus specific humoral response after immunization. After challenge a specific humoral response could be demonstrated in the fish in all groups, but no correlation between this response and protection was found.
Mikalsen
Aase B
AB
Department of Food Safety and Infection Biology, The Norwegian School of Veterinary Science, N-0033 Oslo, Norway. aase.mikalsen@veths.no
Sindre
Hilde
H
Torgersen
Jacob
J
Rimstad
Espen
E
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000517:prevention & control*; D005822:Genetic Vectors; D006321:Heart / Q000821:virology; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D046829:Isavirus / Q000235:genetics / Q000276:immunology*; D009206:Myocardium; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020317:Salmo salar; D016019:Survival Analysis; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D012367:RNA, Viral; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins; C060814:hemagglutinin esterase