TAT-mediated protein transduction: delivering biologically active proteins to the heart.
TAT protein transduction is a novel method of delivering biologically active proteins into cells and tissues through the fusion of a protein transduction domain to the protein of interest. The present chapter outlines the methodology pertaining to the preparation of TAT-fusion proteins and how to efficiently transduce these proteins into cultured cells or isolated rat hearts perfused in Langendorff mode.
Gustafsson
Asa B
AB
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
Gottlieb
Roberta A
RA
Granville
David J
DJ
D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006321:Heart; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D010477:Perfusion; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014161:Transduction, Genetic
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins