Investigations into the electrooxidation of guanosine-5'-triphosphate at the pyrolytic graphite electrode.
The electrochemical oxidation of guanosine-5'-triphosphate has been investigated in phosphate-containing electrolytes in the pH range 1.5-10.9 at a pyrolytic graphite electrode by cyclic sweep voltammetry, spectral studies, bulk electrolysis and related techniques. In this pH range, the oxidation occurred in a single well-defined peak (Ia). The peak potential of oxidation peaks (Ep) was found to be dependent on pH, concentration and sweep rate. The kinetics of the UV-absorbing intermediates was followed spectrophotometrically and the decay of the intermediate occurred in a pseudo-first-order reaction. The first-order rate constants for the disappearance of the UV-absorbing intermediate have also been calculated. The products of the electrode reaction were characterized by HPLC and GC/MS. A tentative mechanism for the formation of the products has also been suggested.
Goyal
Rajendra N
RN
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India. rngcyfcy@iitr.ernet.in
Tyagi
Anuradha
A
D019281:Dimerization; D004563:Electrochemistry; D004566:Electrodes; D004573:Electrolytes; D006108:Graphite / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
D004573:Electrolytes; D006108:Graphite; D006160:Guanosine Triphosphate