Assessment of angiogenic markers in oral hemangiomas and pyogenic granulomas.
The purpose of this research was to evaluate the immunohistochemical expression of the vascular endothelial growth factor (VEGF-C1) and measuring the angiogenic activity by the staining for von Willebrand factor (vWF) and CD31 in oral pyogenic granulomas and hemangiomas. The results showed that there was no statistically significant difference in the angiogenesis index between the lesions evaluated. The average microvessel density determined for MVC (microvessel count) using CD31 was 60.64 for hemangiomas and 59.64 for pyogenic granulomas, while angiogenic index determined using vWF was 64.24 and 62.20 in these lesions. The results showed that the cells highlighted by staining for vWF were more uniform than in those stained for CD31. There was no statistically significant difference between the lesions for the number of cells highlighted by staining for VEGF-C1. However, the mean number of cells highlighted in pyogenic granuloma specimens was higher (153.23) when compared to oral hemangioma specimens (115.17). The VEGF-positive cells were endothelial cells and fibroblasts in hemangiomas and macrophages and fibroblasts in pyogenic granulomas. These results effort the role of the angiogenic factors in the etiopathogenesis of the hemangiomas and pyogenic granulomas, however, it showed that microvessel quantification is not useful in the differential diagnosis of these lesions.
Freitas
Tarsila M C
TM
Dentistry School, Federal University of Rio Grande do Norte, Departamento de Odontologia, Programa de Pós-graduação em Patologia Oral, Av. Salgado Filho 1787, Lagoa Nova, Natal-RN 59056-000, Brazil.
Miguel
Márcia C C
MC
Silveira
Ericka J D
EJ
Freitas
Roseana A
RA
Galvão
Hébel C
HC
D015415:Biomarkers / Q000032:analysis*; D003937:Diagnosis, Differential; D042783:Endothelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D017789:Granuloma, Pyogenic / Q000473:pathology*; D006391:Hemangioma / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D009062:Mouth Neoplasms / Q000098:blood supply* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000096:biosynthesis
D015415:Biomarkers; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042582:Vascular Endothelial Growth Factor C; D014841:von Willebrand Factor