Isolation of a reovirus from a broiler chicken flock with high early mortality.
A reovirus was isolated from a flock of 12,000 broiler chickens which experienced a total mortality of 6.3% up to 35 days. The reovirus produced large syncytia in primary chicken kidney cell cultures with eosinophilic intracytoplasmic inclusion body formation. Infected cells reacted with fluorescein conjugated specific anti-reovirus serum. Negatively strained virions had a double shelled appearance with overall diameter of 65 to 70 nm. The nucleic acid had ten discrete segments with electrophoretic mobilities as would be expected for a reovirus.
Saifuddin
M
M
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
Wilks
C R
CR
Christensen
N H
NH
Fu
Z F
ZF
Rice
M
M