title: Effect of cysteine protease inhibitor Ep-475 on TNF-alpha-independent cyclophosphamide-induced apoptosis in mouse lymphosarcoma LS cells.
abstract: Cyclophosphamide 1.5-2.0-fold increased activity of cathepsins B and L in tumor tissue of mouse lymphosarcoma LS and caused tumor regression. The effect was most pronounced on day 5 after treatment. Twofold treatment with a selective cathepsin inhibitor Ep-475 slightly stimulated tumor growth in control mice and significantly reduced the antitumor effect of cyclophosphamide. Lysosomal cysteine proteases cathepsins B and L are involved, but do not play a key role in TNF-alpha-independent apoptosis in LS cells induced by cyclophosphamide.
authors: Institute of Physiology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk. djsja@mail.ru
authors: S Ya
authors: SY
authors: Zhanaeva
authors: T A
authors: TA
authors: Korolenko
authors: O M
authors: OM
authors: Khoshchenko
authors: V K
authors: VK
authors: Spiridonov
authors: V P
authors: VP
authors: Nikolin
authors: V I
authors: VI
authors: Kaledin
mesh_terms: D000818:Animals; D017209:Apoptosis; D002401:Cathepsin B / Q000037:antagonists & inhibitors*; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors*; D003520:Cyclophosphamide / Q000494:pharmacology; D003546:Cysteine Endopeptidases; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015853:Cysteine Proteinase Inhibitors; D014409:Tumor Necrosis Factor-alpha; C032425:N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine; D003520:Cyclophosphamide; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D007930:Leucine