title: Development of an infection-resistant, bioactive wound dressing surface.
abstract: Trauma, whether caused by an accident or in an intentional manner, results in significant morbidity and mortality. The goal of this study was to develop a novel biomaterial surface in vitro and ex vivo that provides both localized infection resistance nd hemostatic properties. Our hypothesis is that a combination of specific surface characteristics can be successfully incorporated into a single biomaterial. Functional groups were created with woven Dacron (Cntrl) material via exposure to ethylenediamine (C-EDA). The antibiotic ciprofloxacin (Cipro) was then applied to the C-EDA material using pad/autoclave technique (C-EDA-AB) followed by surface immobilization of the coagulation cascade enzyme thrombin (C-EDA-AB-Thrombin). Antimicrobial activity by the C-EDA-AB surface persisted for 5 days compared with Cntrl and dipped controls, which lasted <1 h. C-EDA-AB-Thrombin surfaces had 2.6- and 105-fold greater surface thrombin activity compared with nonspecifically bound thrombin and Cipro-dyed surfaces, respectively. Surface thrombus formation ex vivo was evident after 1 min of exposure, with thrombus organization evident by 2.5 min. In contrast, C-EDA-AB and Cntrl segments showed only blood protein adsorption on the fibers. Thus, this study demonstrated that Cipro and thrombin can be simultaneously incorporated onto a biomaterial surface while maintaining their respective biological activities.
authors: BioSurfaces, 171 Main Street, Suite 302, Ashland, Massachusetts 01721, USA. biosurfaces@verizon.net
authors: Matthew D
authors: MD
authors: Phaneuf
authors: Martin J
authors: MJ
authors: Bide
authors: Susan L
authors: SL
authors: Hannel
authors: Michael J
authors: MJ
authors: Platek
authors: Thomas S
authors: TS
authors: Monahan
authors: Mauricio A
authors: MA
authors: Contreras
authors: Tina M
authors: TM
authors: Phaneuf
authors: Frank W
authors: FW
authors: LoGerfo
mesh_terms: D001424:Bacterial Infections / Q000517:prevention & control*; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D002939:Ciprofloxacin / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry; D014947:Wounds and Injuries
chemical_list: D001672:Biocompatible Materials; D005029:Ethylenediamines; D011093:Polyethylene Terephthalates; D002939:Ciprofloxacin; C031234:ethylenediamine; D013917:Thrombin