Polyelectrolyte complex micelles composed of c-raf antisense oligodeoxynucleotide-poly(ethylene glycol) conjugate and poly(ethylenimine): effect of systemic administration on tumor growth.
An antisense oligodeoxynucleotide (ODN) delivery system based on polyelectrolyte complex (PEC) micelles composed of an ODN-poly(ethylene glycol) (PEG) conjugate and polyethylenimine (PEI) was demonstrated. The PEC micelles having a core/shell structure were spontaneously formed in an aqueous solution by ionic interactions between ODN part in the conjugate and PEI. The ODN/PEI polyelectrolyte complex formed an inner core while PEG chains surrounded it as a shell. The morphology of the micelles was visualized as a separate sphere by atomic force microscopy (AFM). When the micelles containing a c-raf antisense ODN were intravenously administered into tumor-bearing nude mice, significant antitumor activities against human lung cancer were observed. The intravenously injected micelles also showed significantly higher accumulation level in the solid tumor region compared to that of naked ODN.
Jeong
Ji Hoon
JH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea (South.
Kim
Sun Hwa
SH
Kim
Sung Wan
SW
Park
Tae Gwan
TG
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004573:Electrolytes / Q000737:chemistry*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D018625:Microscopy, Atomic Force; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D019908:Proto-Oncogene Proteins c-raf / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology
D004573:Electrolytes; D008823:Micelles; D016376:Oligonucleotides, Antisense; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D019908:Proto-Oncogene Proteins c-raf