title: Androgen regulation of gene expression in the mouse lacrimal gland.
abstract: The objective of this study was to determine the nature and extent of androgen influence on gene expression in the lacrimal gland. Lacrimal glands were obtained from orchiectomized mice that had been treated with testosterone or vehicle for 2 weeks, as well as from testicular feminized mice and their Tabby controls. Samples were pooled according to experiment, processed for the isolation of RNA, and analyzed for differentially expressed mRNAs by using primarily CodeLink Bioarrays, GEM 1 and 2 gene chips and quantitative real-time PCR (qPCR) procedures. Gene chip data were analyzed with GeneSifter.Net software. Our results demonstrate that testosterone regulates the expression of over 2000 genes in the lacrimal gland. Gene ontologies most affected by androgen treatment included those related to cell growth, proliferation and metabolism, cell communication and transport, nucleic acid binding, signal transduction and receptor activities. Our findings also indicate that androgen action may be mediated, at least in part, through classical androgen receptors, and may contribute to the sex-related differences in gene expression of lacrimal tissue. Overall, these results support our working hypothesis that androgen action on the lacrimal gland is mediated primarily through a receptor-associated regulation of gene transcription.
authors: Schepens Eye Research Institute, 20 Staniford Street, Boston, MA 02114, USA.
authors: Stephen M
authors: SM
authors: Richards
authors: Meng
authors: M
authors: Liu
authors: Roderick V
authors: RV
authors: Jensen
authors: Frank
authors: F
authors: Schirra
authors: Hiroko
authors: H
authors: Yamagami
authors: Michael J
authors: MJ
authors: Lombardi
authors: Patricia
authors: P
authors: Rowley
authors: Nathaniel S
authors: NS
authors: Treister
authors: Tomo
authors: T
authors: Suzuki
authors: Benjamin D
authors: BD
authors: Sullivan
authors: David A
authors: DA
authors: Sullivan
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007765:Lacrimal Apparatus / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009919:Orchiectomy; D012333:RNA, Messenger / Q000378:metabolism; D012727:Sex Characteristics; D013739:Testosterone / Q000502:physiology; D015854:Up-Regulation
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D013739:Testosterone