Myostatin gene silenced by RNAi show a zebrafish giant phenotype.
Myostatin is a member of the transforming growth factor-beta (TGF-beta) family that functions as a negative regulator of skeletal muscle development and growth. Recently, it has been reported that the transgenic zebrafish expressing myostatin prodomain exhibited an increased number of fiber in skeletal muscle. Other novel results suggest that myostatin plays a mayor role during myogenesis, apart from inhibition of proliferation as well as differentiation. We have investigated the ability of double-stranded RNA (dsRNA) to inhibit myostatin function in the zebrafish. By microinjection dsRNA, corresponding to biologically active C-terminal domain from aminoacid 268 to end codon of tilapia myostatin protein, we produced an increased body mass in treated fish. The dsRNA injection in early development stage in zebrafish produced hyperplasia or hypertrophy. In addition, the interference of gene function showed a strong dependence on the amount of dsRNA.
Acosta
Jannel
J
Aquatics Biotechnology Project, Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box. 6162, Havana 10600, Cuba.
Carpio
Yamila
Y
Borroto
Ingrid
I
González
Osmany
O
Estrada
Mario Pablo
MP
D000818:Animals; D001835:Body Weight / Q000235:genetics; D020868:Gene Silencing; D006965:Hyperplasia / Q000235:genetics / Q000378:metabolism; D006984:Hypertrophy / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000473:pathology* / Q000503:physiopathology*; D055435:Myostatin; D009929:Organ Size / Q000235:genetics; D010641:Phenotype; D034622:RNA Interference; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism*; D029961:Zebrafish Proteins
C525914:Mstnb protein, zebrafish; D055435:Myostatin; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins