[Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber)].
A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific bands.
Chen
Hua
H
Key Laboratory of Phytosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093.
Li
Ping
P
Liu
Jing
J
Li
Yin-Xin
YX
D003470:Culture Media; D018525:Germination / Q000502:physiology*; D018515:Plant Leaves / Q000254:growth & development*; D012038:Regeneration; D031205:Taraxacum / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media