title: The possibility of discriminating within the Bacillus cereus group using gyrB sequencing and PCR-RFLP.
abstract: Based on a combination of PCR and restriction endonuclease (RE) digestion (PCR-RE digestion), we have examined the possibility of differentiating members of the Bacillus cereus group. Fragments of the gyrB gene (362 bp) from pure cultures of 12 B. cereus, 25 B. thuringiensis, 25 B. mycoides and two B. anthracis strains were amplified and subsequently digested with Sau3A1. Furthermore, a majority of the amplicons were sequenced directly to verify the PCR-RE results. The results obtained suggest that only the B. mycoides generates specific fragments following PCR-RE. Conversely, it was not possible to discriminate between the B. cereus and the B. thuringiensis strains using the methods described.
authors: Thomsen Bioscience A/S, Strømmen 6, DK-9400 Nørresundby, Denmark. gbj@thomsen-bioscience.com
authors: Gert B
authors: GB
authors: Jensen
authors: Niels
authors: N
authors: Fisker
authors: Thomas
authors: T
authors: Sparsø
authors: Lars
authors: L
authors: Andrup
mesh_terms: D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D005516:Food Microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D027081:DNA Gyrase