title: Acute microvascular platelet aggregation after subarachnoid hemorrhage.
abstract: OBJECTIVE
  The mechanisms underlying acute cerebral ischemia after subarachnoid hemorrhage (SAH) are not well established. Platelets aggregate within major cerebral vessels hours after SAH, but this has not been studied in the microvasculature. Platelet aggregates within the microvasculature could mechanically obstruct the lumen and initiate events that injure vessel structure. In the present study the authors examined the hypothesis that platelets aggregate within the cerebral microvasculature acutely after SAH.
  
  
  METHODS
  Subarachnoid hemorrhage was induced in the rat by using the endovascular perforation model. The animals were killed between 10 minutes and 48 hours after SAH. Immunostaining for the platelet surface receptor glycoprotein (GP)IIb/IIIa, which mediates platelet aggregation, was used to detect platelet aggregation. Sham-operated animals were used as controls. The GPIIb/IIIa immunoreactive platelet aggregates were abundant in the microvasculature of the basal and frontal cortex, striatum, and hippocampus 10 minutes after SAH. These aggregates decreased in number from 1 to 6 hours post-SAH and then increased to a peak at 24 hours. No immunoreactive aggregates were observed 48 hours after SAH.
  
  
  CONCLUSIONS
  The data indicate that widespread platelet aggregation occurs very rapidly in response to SAH followed by a decrease within 6 hours and a subsequent increase 24 hours after SAH. Microvascular platelet aggregates may contribute to decreased cerebral blood flow and ischemic injury after SAH via a number of mechanisms.
authors: Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029-6574, USA. fatima.sehba@mssm.edu
authors: Fatima A
authors: FA
authors: Sehba
authors: Gulam
authors: G
authors: Mostafa
authors: Victor
authors: V
authors: Friedrich
authors: Joshua B
authors: JB
authors: Bederson
mesh_terms: D000208:Acute Disease; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D001794:Blood Pressure; D002545:Brain Ischemia / Q000097:blood / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D007427:Intracranial Pressure; D008297:Male; D008833:Microcirculation; D010974:Platelet Aggregation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000097:blood / Q000503:physiopathology*
chemical_list: D019039:Platelet Glycoprotein GPIIb-IIIa Complex