Pregnancies and births resulting from in vitro matured oocytes fertilized with testicular spermatozoa.
OBJECTIVE
In vitro maturation (IVM) of immature human oocytes is an attractive option for the treatment of infertility. Similarly, intracytoplasmic sperm injection (ICSI) followed by testicular fine needle aspiration (TEFNA) is an important treatment for primarily male-factor infertility. This report highlights the combination of these two advanced assisted reproduction techniques, namely IVM and fertilization with TEFNA-retrieved spermatozoa by ICSI to overcome both of male and female infertility problems.


METHODS
Before immature oocyte retrieval (IOR), gonadotropin stimulation was given for 3 or 5 days. Following IVM, and mature oocytes were inseminated by ICSI followed by TEFNA.


RESULTS
Four couples with five completed treatment cycles were performed, and total of 36 immature oocytes were retrieved. Following 36 to 48 h of culture, 32 (88.89%, 32/36) oocytes became mature. The mature oocytes were inseminated with TEFNA-retrieved sperm, and 18 (56.25%, 18/32) oocytes were fertilized normally following ICSI. Eleven embryos were transferred in five cycles and two pregnancies and two singleton births were achieved in two patients.


CONCLUSIONS
This result demonstrates that the successful pregnancies and live births can be established from embryos produced from in vitro matured oocytes that fertilized with testicular sperm.
Yun
Qian
Q
Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, P R China. qianyun@njmu.edu.cn
Ting
Feng
F
Chen
Juan
J
Cai
Ling-Bo
LB
Liu
Jia-Yin
JY
Mao
Yun-Dong
YD
Ding
Wei
W
Sha
Jia-Hao
JH
D000328:Adult; D018929:Cell Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D006062:Gonadotropins / Q000502:physiology; D006801:Humans; D008297:Male; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis
D006062:Gonadotropins