title: Syntheses and DNA-binding studies of two ruthenium(II) complexes containing one ancillary ligand of bpy or phen: [Ru(bpy)(pp[2,3]p)2](ClO4)2 and [Ru(phen)(pp[2,3]p)2](ClO4)2.
abstract: Two new ruthenium(II) complexes of [Ru(bpy)(pp[2,3]p)2](ClO4)2 and [Ru(phen)(pp[2,3]p)2](ClO4)(2) (bpy=2,2'-bipyridine, phen=1,10-phenanthroline, pp[2,3]p=pyrido[2',3':5,6]pyrazino[2,3-f][1,10]phenanthroline) have been synthesized and characterized by elemental analysis and 1H NMR spectra. The calf thymus DNA-binding properties of the two complexes were investigated by UV-visible and emission spectroscopy, competitive binding experiments with ethidium bromide and viscosity measurements. The results indicate that the two complexes intercalate between the base pairs of the DNA tightly with intrinsic DNA-binding constants of 3.08 x 10(6) and 6.53 x 10(6) M(-1) in buffered 50 mM NaCl, respectively, which are much larger than 6.9 x 10(5) M(-1) for [Ru(bpy)2(pp[2,3]p)](ClO4)2 containing two ancillary ligands of bpy.
authors: Department of Chemistry, Beijing Normal University, Xinjiecouwai Street 19, Beijing 100875, China.
authors: Bao-Yan
authors: BY
authors: Wu
authors: Li-Hua
authors: LH
authors: Gao
authors: Zhi-Ming
authors: ZM
authors: Duan
authors: Ke-Zhi
authors: KZ
authors: Wang
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D008163:Luminescent Measurements; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011522:Protons; D017975:Ruthenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D014783:Viscosity
chemical_list: C504122:(Ru(bpy)(pp(2,3)p)2)(ClO4)2; C504121:(Ru(phen)(pp(2,3)p)2)(ClO4)2; D008024:Ligands; D009942:Organometallic Compounds; D011522:Protons; D017975:Ruthenium Compounds; D004247:DNA; C427203:calf thymus DNA