Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.
A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.
Emmerechts
Gert
G
Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Herdewijn
Piet
P
Rozenski
Jef
J
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D011560:Pseudouridine / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000737:chemistry*
D013450:Sulfones; D011560:Pseudouridine; C060916:methyl vinyl sulfone