Syntheses and DNA-binding studies of two ruthenium(II) complexes containing one ancillary ligand of bpy or phen: [Ru(bpy)(pp[2,3]p)2](ClO4)2 and [Ru(phen)(pp[2,3]p)2](ClO4)2.
Two new ruthenium(II) complexes of [Ru(bpy)(pp[2,3]p)2](ClO4)2 and [Ru(phen)(pp[2,3]p)2](ClO4)(2) (bpy=2,2'-bipyridine, phen=1,10-phenanthroline, pp[2,3]p=pyrido[2',3':5,6]pyrazino[2,3-f][1,10]phenanthroline) have been synthesized and characterized by elemental analysis and 1H NMR spectra. The calf thymus DNA-binding properties of the two complexes were investigated by UV-visible and emission spectroscopy, competitive binding experiments with ethidium bromide and viscosity measurements. The results indicate that the two complexes intercalate between the base pairs of the DNA tightly with intrinsic DNA-binding constants of 3.08 x 10(6) and 6.53 x 10(6) M(-1) in buffered 50 mM NaCl, respectively, which are much larger than 6.9 x 10(5) M(-1) for [Ru(bpy)2(pp[2,3]p)](ClO4)2 containing two ancillary ligands of bpy.
Wu
Bao-Yan
BY
Department of Chemistry, Beijing Normal University, Xinjiecouwai Street 19, Beijing 100875, China.
Gao
Li-Hua
LH
Duan
Zhi-Ming
ZM
Wang
Ke-Zhi
KZ
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D008163:Luminescent Measurements; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011522:Protons; D017975:Ruthenium Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D014783:Viscosity
C504122:(Ru(bpy)(pp(2,3)p)2)(ClO4)2; C504121:(Ru(phen)(pp(2,3)p)2)(ClO4)2; D008024:Ligands; D009942:Organometallic Compounds; D011522:Protons; D017975:Ruthenium Compounds; D004247:DNA; C427203:calf thymus DNA