Blood-derived dendritic cells in an acute brain injury.
Dendritic cells (DC), while absent from the normal CNS parenchyma, have been demonstrated in delayed type hypersensitivity (DTH), experimental allergic encephalomyelitis (EAE), and some MS lesions. We demonstrate that an acute excitotoxic lesion results in DC recruitment to the brain, with maximal cells between days 3 and 5. Clodronate liposome-mediated depletion of cerebral perivascular macrophages does not prevent recruitment of DC; however, a lesion in a whole-body irradiated animal does not show evidence of DC recruitment. We conclude that DC are recruited to an excitotoxic lesion from a blood-derived cell population. This may have implications for neuropathologies involving DC recruitment.
Newman
Tracey A
TA
CNS Inflammation Group, University of Southampton, UK. tan@soton.ac.uk
Galea
Ian
I
van Rooijen
Nico
N
Perry
V Hugh
VH
D000208:Acute Disease; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000473:pathology / Q000528:radiation effects; D001927:Brain Diseases / Q000097:blood* / Q000139:chemically induced / Q000473:pathology*; D002460:Cell Line; D003713:Dendritic Cells / Q000473:pathology*; D018690:Excitatory Amino Acid Agonists; D007608:Kainic Acid; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014916:Whole-Body Irradiation
D000943:Antigens, Differentiation; D015415:Biomarkers; D018690:Excitatory Amino Acid Agonists; C459465:OX-62 antigen, rat; D007608:Kainic Acid