title: Surface-initiated growth of poly d(A-T) by Taq DNA polymerase.
abstract: In this paper, we report surface-initiated d(A-T) polymerization by Taq DNA polymerase as a method for constructing DNA-tethered surfaces using an enzyme. The enzymatic polymerization was conducted successfully via two steps: tethering of oligo d(A-T)s onto the surface presenting carboxylic acids by amide coupling and surface-initiated polymerization using Taq DNA polymerase. In this enzymatic polymerization process, the design and construction of carboxylic acid-presenting surfaces were found to be an important factor: DNA growth did not occur on the gold surface coated only with the self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA), but effectively proceeded on the surfaces presenting mixed SAMs of MHDA and 1-pentadecanethiol. The coupling of oligo d(A-T)s and the subsequent DNA polymerization reaction were characterized by polarized infrared external reflectance spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy.
authors: Department of Chemistry and School of Molecular Science (BK21), Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 Korea.
authors: Young Shik
authors: YS
authors: Chi
authors: Young Hwan
authors: YH
authors: Jung
authors: Insung S
authors: IS
authors: Choi
authors: Yang-Gyun
authors: YG
authors: Kim
mesh_terms: D006046:Gold; D018625:Microscopy, Atomic Force; D010169:Palmitic Acids; D011067:Poly dA-dT / Q000096:biosynthesis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013057:Spectrum Analysis; D013499:Surface Properties; D019914:Taq Polymerase / Q000737:chemistry / Q000378:metabolism; D014965:X-Rays
chemical_list: C450319:16-mercaptohexadecanoic acid; D010169:Palmitic Acids; D011067:Poly dA-dT; D006046:Gold; D019914:Taq Polymerase