The uses of AFLP for detecting DNA polymorphism, genotype identification and genetic diversity between yeasts isolated from Mexican agave-distilled beverages and from grape musts.
OBJECTIVE
The objectives were to determine the variability and to compare the genetic diversity obtained using amplified fragment length polymorphism (AFLP) markers in analyses of wine, tequila, mezcal, sotol and raicilla yeasts.


RESULTS
A molecular characterization of yeasts isolated from Mexican agave musts, has been performed by AFLP marker analysis, using reference wine strains from Italian and South African regions.


CONCLUSIONS
A direct co-relation between genetic profile, origin and fermentation process of strains was found especially in strains isolated from agave must. In addition, unique molecular markers were obtained for all the strains using six combination primers, confirming the discriminatory power of AFLP markers.


CONCLUSIONS
This is the first report of molecular characterization between yeasts isolated from different Mexican traditional agave-distilled beverages, which shows high genetic differences with respect to wine strains.
Flores Berrios
E P
EP
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Jalisco, México. eflores@ciatej.net.mx
Alba González
J F
JF
Arrizon Gaviño
J P
JP
Romano
P
P
Capece
A
A
Gschaedler Mathis
A
A
D027882:Agave / Q000737:chemistry / Q000382:microbiology; D000434:Alcoholic Beverages / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D005838:Genotype; D008800:Mexico; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D027843:Vitis / Q000737:chemistry / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
D004271:DNA, Fungal; D012337:RNA, Ribosomal, 18S