[Determination of complanatoside A in semen Astragali complanati by HPLC].
OBJECTIVE
To establish the determination method for complanatoside A in seeds of Astragalus complanatus.


METHODS
An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.


RESULTS
The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.


CONCLUSIONS
The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.
Zhang
Jian-Jun
JJ
Beijing University of Chinese Traditional Medicine, Beijing 100029, China.
Yan
Xing-Li
XL
Zhang
Yu-Jie
YJ
Wang
Yu-Ping
YP
Zhang
Qiu-Ju
QJ
Zeng
Feng-Ying
FY
Li
Wei
W
Ou
Li-Na
LN
D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044948:Flavonols / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012639:Seeds / Q000737:chemistry
D044948:Flavonols; D005960:Glucosides; C057070:complanatuside