Whole-genome allelotyping using laser microdissected tissue.
Laser-based microdissection technologies have been recently developed and applied to procure homogenous populations of tumor cells from paraffin-embedded and frozen tissue sections. When combined with whole-genomic amplification techniques, sufficient amounts of DNA can be generated from a small number of tumor cells procured by laser-based microdissection. Amplified DNA can then be used to perform high-throughput genome-wide allelotyping using fluorescent-labeled microsatellite markers spanning the whole genome. Loss of heterozygosity can be assessed by Genescan and Genotyper software (ABI Prism).
Feltmate
Colleen M
CM
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Mok
Samuel C
SC
D000483:Alleles; D005260:Female; D015894:Genome, Human; D006801:Humans; D007834:Lasers; D019656:Loss of Heterozygosity / Q000235:genetics; D042282:Microdissection / Q000379:methods*; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology