Isolation of equine herpesvirus-1 lacking glycoprotein C from a dead neonatal foal in Japan.
We isolated a variant equine herpesvirus-1 (EHV-1), strain 5089, from the lung of a dead neonatal foal in Japan and characterized the biological nature of the virus. The virus spread in cultured cells mainly by cell-to-cell infection, unlike wild-type EHV-1, which spreads efficiently as a cell-free virus. The virus titer in cultured supernatant and the intracellular virus titer were low compared to those of wild-type EHV-1. Heparin treatment of the virus had no effect on viral infectivity in cell culture. Glycoprotein C (gC) was not detected by Western blotting and fluorescent antibody tests in 5089 virions and 5089-infected cells, respectively. RT-PCR analysis revealed that the expression level of 5089 gC mRNA was reduced considerably compared to that of wild-type EHV-1. Sequencing analysis of the 5089 gC coding region showed a point mutation in the promoter region of the gC open reading frame. However, the mutation did not affect the promoter activity. These results suggested that the lack of gC in 5089 virions might be one of the reasons for spread of the virus by cell-to-cell infection and that gC mRNA expression might not be activated efficiently due to factors other than the mutation in the gC promoter region.
Kirisawa
R
R
Department of Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan. r-kirisa@rakuno.ac.jp
Hosoi
Y
Y
Yamaya
R
R
Taniyama
H
H
Okamoto
M
M
Tsunoda
N
N
Hagiwara
K
K
Iwai
H
H
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000302:isolation & purification*; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D007564:Japan; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis
D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C080410:glycoprotein C, Equid herpesvirus 1