A biological and molecular characterization of some Egyptian barley genotypes which are resistant to net blotch disease.
A survey for resistance against net blotch disease (caused by Pyrenophora teres) was performed on some Egyptian barley landraces and some selected resistance and susceptible standard German barley genotypes. The results indicated that most of the Egyptian barley landraces are extremely resistant to the disease. Molecular analysis using RAPD and AFLP showed unique banding profiles for the different genotypes, and specific AFLP markers for the Egyptian genotypes were identified. The effectiveness of RAPD and AFLP for identifying different barley genotypes of different origins and with different reactions against P. teres was discussed. The results of the biological evaluation and molecular characterization done in this study can be seen as the starting point needed to identify the valuable net blotch resistant Egyptian barley germplasm at both the phenotype and genotype levels and draw the attention of breeders and banks of natural plant genetic resources towards this valuable yet neglected germplasm.
Saker
Mahmoud M
MM
Plant Biotechnology Dept., National Research Centre, Dokki, Cairo, Egypt. Sakrmahmoud@yahoo.com
D001203:Ascomycota / Q000502:physiology*; D005838:Genotype; D001467:Hordeum / Q000235:genetics* / Q000502:physiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length