title: A genetically engineered strain of Pseudomonas putida as a useful tool for identifying new therapeutic herbicides.
abstract: A genetically engineered strain of Pseudomonas putida U designed for the identification of new therapeutic herbicides has been obtained. In this bacterium, deletion of the homogentisate gene cluster (hmgRABC) confers upon this mutant huge biotechnological possibilities since it can be used: (i) as a target for testing new specific herbicides (p-hydroxy-phenylpyruvate dioxygenase inhibitors); (ii) to identify new therapeutic drugs-effective in the treatment of alkaptonuria and other related tyrosinemia - and (iii) as a source of homogentisic acid in a plant-bacterium association.
authors: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, España, Spain.
authors: Elsa
authors: E
authors: Arias-Barrau
authors: Angel
authors: A
authors: Sandoval
authors: Mario
authors: M
authors: Arcos
authors: Sagrario
authors: S
authors: Arias
authors: Germán
authors: G
authors: Naharro
authors: Elías R
authors: ER
authors: Olivera
authors: José M
authors: JM
authors: Luengo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005818:Genetic Engineering / Q000379:methods; D006540:Herbicides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D016958:Pseudomonas putida / Q000187:drug effects / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D006540:Herbicides