title: Protective effect of egg yolk phosvitin against ultraviolet- light-induced lipid peroxidation in the presence of iron ions.
abstract: It is known that nonheme iron accumulates and free radicals are generated in skin exposed to ultraviolet (UV) light. Iron ions have a role in skin photodamage by participating in the formation of reactive oxygen species. In this study, we evaluated the effect of egg yolk phosvitin on UV-light-induced oxidative stress. Mouse dorsal skin homogenate was exposed to UVA light in the presence or absence of ferric nitrilotriacetate (FeNTA). Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances (TBARS). The TBARS concentration increased with increasing FeNTA concentration and UV-light-exposure time. In the presence of FeNTA, phosvitin more effectively inhibited in vitro lipid peroxidation than did bovine serum albumin. According to results of electron spin resonance studies using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent, phosvitin suppressed the formation of hydroxyl radicals. These results suggest that UV-light-induced oxidative stress can be reduced by phosvitin.
authors: Faculty of Animal Science, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
authors: Shin-ichi
authors: S
authors: Ishikawa
authors: Shizuna
authors: S
authors: Ohtsuki
authors: Kentarou
authors: K
authors: Tomita
authors: Keizo
authors: K
authors: Arihara
authors: Makoto
authors: M
authors: Itoh
mesh_terms: D000818:Animals; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D017665:Hydroxyl Radical; D007477:Ions; D007501:Iron / Q000378:metabolism*; D015227:Lipid Peroxidation; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress; D010774:Phosvitin / Q000378:metabolism / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D019097:Spin Trapping; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D003497:Cyclic N-Oxides; D007477:Ions; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D010774:Phosvitin; D007501:Iron