title: Molecular cloning of a novel LOS2 gene from Capsella bursa-pastoris.
abstract: A new LOS2 gene was cloned from C. bursa-pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA of C. bursa-pastoris LOS2 gene (designated as Cblos2) was 1694 bp containing a 1332 bp open reading frame (ORF) encoding a 444 amino acid protein. The predicted CbLOS2 protein contained enolase-N domain, enolase domain, conserved putative DNA-binding and repression domains like LOS2 from A. thaliana. Bioinformatic analysis indicated that CbLOS2 had similarity with other enolase proteins. Cold acclimation assay revealed that Cblos2 expressed constitutively in C. bursa-pastoris and was involved in the cold acclimation process, implying CbLOS2 was a bi-functional enolase.
authors: State Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R & D Center, Morgan-Tan International Center for Life Sciences, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors: Si-Xiu
authors: SX
authors: Liu
authors: Xing-Long
authors: XL
authors: Wang
authors: Jin-Shui
authors: JS
authors: Yang
authors: Zheng-Qiu
authors: ZQ
authors: Fan
authors: Xiao-Fen
authors: XF
authors: Sun
authors: Xiang-Rong
authors: XR
authors: Wang
authors: Ke-Xuan
authors: KX
authors: Tang
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000595:Amino Acid Sequence; D029687:Capsella / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D010751:Phosphopyruvate Hydratase