CD8+ T-cell tolerance induced by delivery of antigen to the anterior chamber is not the result of de facto intravenous or mucosal administration of antigen.
OBJECTIVE
We tested whether antigen administration via the anterior chamber (a.c.) was equivalent to intravenous (i.v.) or mucosal administration antigen.


METHODS
Ovalbumin (OVA)-specific CD8(+) T cells (OT-I) were enumerated in lymphoid tissues of C57Bl/6 (B6) mice via adoptive transfer after the same amount of antigen was administered via a.c., i.v., or mucosal routes. Lytic activity was measured in B6 and gammadeltaT cell-deficient B6 mice given OVA via a.c., i.v, or mucosal routes after injection with OVA in adjuvant.


RESULTS
OVA a.c. induced a pattern of T-cell proliferation distinct from i.v. or mucosal administration. A.c. and i.v., but not mucosal, OVA induced cytolytic T lymphocyte (CTL) tolerance. The inhibition of CTL responses was significantly greater in mice given OVA a.c. rather than i.v. gammadeltaT cells contributed to a.c.-, but not i.v.-, induced CTL tolerance.


CONCLUSIONS
A.c. administration of antigen not de-facto i.v. or mucosal administration of antigen.
McKenna
Kyle C
KC
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA, USA.
Anderson
Kimberly M
KM
Kapp
Judith A
JA
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000867:Anterior Chamber / Q000166:cytology / Q000276:immunology*; D016827:CD8 Antigens / Q000008:administration & dosage*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D007108:Immune Tolerance / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007267:Injections / Q000379:methods; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000276:Adjuvants, Immunologic; D016827:CD8 Antigens; D010047:Ovalbumin