title: Identification of cellular membrane proteins interacting with hepatitis B surface antigen using yeast split-ubiquitin system.
abstract: Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These include a resident protein of endoplasmic reticulum (thioredoxin-related transmembrane protein 2), an adaptor protein involved in clathrin-mediated endocytosis and HIV-mediated downregulation of CD4, and a co-receptor of coxsakie B virus. The significance of our findings is suggested by the identification of cellular membrane proteins interacting with other virus proteins. Further functional analysis of these HBsAg- interacting cellular membrane proteins should shed new insights on their role in HBV morphogenesis.
authors: Hepatitis Viruses and Liver Cancer Research Laboratory, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive 05N-10, Singapore 637551.
authors: Qi Chun
authors: QC
authors: Toh
authors: Tuan Lin
authors: TL
authors: Tan
authors: Wei Qiang
authors: WQ
authors: Teo
authors: Chin Yee
authors: CY
authors: Ho
authors: Subhajeet
authors: S
authors: Parida
authors: Wei Ning
authors: WN
authors: Chen