Effect of water-soluble proteoglycan isolated from Agaricus blazei on the maturation of murine bone marrow-derived dendritic cells.
Water-soluble proteoglycan (WSPG) of Agaricus blazei Murill has been known to stimulate the non-specific complements and humoral immune functions to act as polyclonal activators of B cells and to inhibit tumor growth and metastasis. However, little is known about its immunomodulating effects on murine bone marrow (BM)-derived dendritic cells (DC). In the present study, we examined the maturation process of murine BM-DC. BM cells were cultured in the presence of IL-4 and GM-CSF and the generated immature DC were stimulated with WSPG or LPS (WSPG-DC and LPS-DC, respectively) for 24 h. WSPG significantly enhanced the expression of co-stimulatory molecules (CD80 and CD86) and major histocompatibility complex (MHC) II, as did LPS. IL-12p70 production in WSPG-DC was also identical to that in LPS-DC. The antigen-uptake capacity of WSPG-DC was determined by FITC-labeled dextran uptake. WSPG-DC lost dextran uptake capacity comparable to LPS-DC. The antigen-presenting capacity of WSPG-DC as analyzed by allogeneic T cell proliferation was significantly increased in comparison with immature DC, was identical to LPS-DC, and induced higher levels of IL-2 in responding T cells. These results indicate the immunomodulatory properties of WSPG, which might be therapeutically useful in the control of cancers and immunodeficient diseases through the up-regulation of DC maturation.
Kim
Gi-Young
GY
Department of Microbiology and Medical Research Institute, Pusan National University, College of Medicine, Ami-dong 1-10, Seo-gu, Pusan 602-739, Republic of Korea.
Lee
Moo-Yeol
MY
Lee
Hee-Jeong
HJ
Moon
Dong-Oh
DO
Lee
Chang-Min
CM
Jin
Cheng-Yun
CY
Choi
Yung Hyun
YH
Jeong
Yong-Kee
YK
Chung
Kyung Tae
KT
Lee
Jae-Yun
JY
Choi
In-Hak
IH
Park
Yeong-Min
YM
D000364:Agaricus; D000818:Animals; D018122:B7-1 Antigen / Q000096:biosynthesis; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011509:Proteoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D014867:Water
D018122:B7-1 Antigen; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011509:Proteoglycans; D014867:Water; D018664:Interleukin-12