title: Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica.
abstract: Genes of Yersiniae spp. involved in production of the siderophore yersiniabactin are located on the high-pathogenicity island (HPI). Their transcription is controlled by the AraC/XilS-like transcriptional regulator YbtA encoded within the HPI. YbtA-regulated divergent and overlapping ybtA and irp6 promoters contain three YbtA binding sites, RS1, RS2 and RS3. Deleting RSs systematically and using ybtA and irp6 transcriptional fusions, we determined that different modes of YbtA binding are responsible for activation of irp6 and repression of ybtA. Based on these data, we propose a model of irp6 and ybtA promoter regulation.
authors: Max von Pettenkofer-Institute of Hygiene and Medical Microbiology Pettenkofer Strasse 9a, 80336 Munich, Germany. anirom@umich.edu
authors: Roman
authors: R
authors: Anisimov
authors: Daniela
authors: D
authors: Brem
authors: Jürgen
authors: J
authors: Heesemann
authors: Alexander
authors: A
authors: Rakin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D044404:Genomic Islands; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017384:Sequence Deletion; D017262:Siderophores / Q000096:biosynthesis / Q000235:genetics; D013844:Thiazoles / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010636:Phenols; D017262:Siderophores; D013844:Thiazoles; D015534:Trans-Activators; C104398:yersiniabactin