[Antologus and heterotopic splenic regeneration in rats].
OBJECTIVE
verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats.


METHODS
forty four Wistar rats were assigned to one of two groups A (n=22) and B (n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1 (n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p => 0.05) were used for.


RESULTS
there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p = 0.182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group.


CONCLUSIONS
regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen.
de Souza
José Carlos Lacerda
JC
Programa de Pós-Graduação em Cirurgia da Faculdade de Medicina da Santa Casa de São Paulo. jclsouza@londrina.net
Athiê
Emilio
E
Marigo
Carlos
C
Rahal
Fares
F
Fagundes
Djalma José
DJ
D000818:Animals; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D013154:Spleen / Q000502:physiology* / Q000637:transplantation; D013156:Splenectomy; D014182:Transplantation, Autologous