[Molecular identity of double-stranded RNA-elements of viral nature isolated from the sugar beet].
Hybridization analysis of the double-strand RNA-elements isolated from the sugar beet of the Kyiv, Vinnytsya and Poltava Regions was carried out. A positive signal has been obtained with the dsRNA fragment, 1.9 kbp long, which codes the viral RNA- dependent RNA-polymerase having high level of homology with the analogous enzyme of mycoviruses from Partitiviridae family. Molecular identity of these dsRNA-elements in the sugar beet samples from the Vinnytsya and Poltava regions has been shown The RT-PCR test system has been developed for detecting the nondescribed mycovirus which is proposed to be called Helicobasidium purpureum partitivirus.
Mel'nichuk
M D
MD
Spiridonov
V G
VG
D000595:Amino Acid Sequence; D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology*; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000737:chemistry / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase