Detection of d-Serine in rat brain by capillary electrophoresis with laser induced fluorescence detection.
A capillary zone electrophoresis method with laser induced fluorescence detection for the chiral separation of highly fluorescent enantiomeric derivatives of d/l-Serine from 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-d/l-Serine) was developed and optimized. Enantiomeric separation of NBD-d/l-Serine was accomplished by using 40 mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD) contained in 100 mM borate buffer, pH 10.0. A 70 cm (effective length of 50 cm) uncoated fused-silica capillary at a voltage of 15 kV was used for the separation. The optimized electrophoretic conditions were subsequently applied to the analysis of d-Serine in rat brain, and satisfactory analytical results with respect to accuracy were obtained. This assay showed acceptable precision, with linearity in the d-Serine concentration range of 0.2-20.0 microM. The limit of detection for d-Serine was 3.0 x 10(-7)M.
Zhao
Shulin
S
College of Chemical Engineering, Sichuan University, Chengdu 610065, China. zhaoshulin001@163.com
Yuan
Hongyan
H
Xiao
Dan
D
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D008297:Male; D051381:Rats; D012694:Serine / Q000032:analysis*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D047392:beta-Cyclodextrins; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D012694:Serine; D009327:4-Chloro-7-nitrobenzofurazan; C031215:betadex