[Experimental study of an animal model of diffuse panbronchiolitis].
OBJECTIVE
To establish an animal model of diffuse panbronchiolitis (DPB) and to investigate the pathogenesis of DPB.


METHODS
Sprague-Dawley (SD) rats were intubated with a silicone tube pre-coated with Pseudomonas aeruginosa (P. aeruginosa) in the bronchus. After sixty days, the pathological changes of lung tissue were analyzed.


RESULTS
Pathological changes characteristic of DPB were found in the SD rats after the placement of a silicate tube pre-coated with P. aeruginosa in the bronchus. The lung pathology was characterized by diffuse inflammation around bronchi and bronchioles in both lungs, and thickening of the walls of bronchioles with infiltration of lymphocytes, plasma cells, and histiocytes. In addition, bronchus-associated lymphoid tissue (BALT) hyperplasia and accumulation of foamy cells were observed around the bronchi. Thickened walls and enlarged lumen of pulmonary arteries were also noticed occasionally.


CONCLUSIONS
The SD rat model of DPB could be established by infection of P. aeruginosa.
Wu
Xiu-hua
XH
Department of Pulmonary Medicine, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
Shen
Ce
C
D000818:Animals; D001988:Bronchiolitis / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D011552:Pseudomonas Infections; D011550:Pseudomonas aeruginosa; D051381:Rats; D017207:Rats, Sprague-Dawley