title: De novo alanine synthesis by bacteroids of Mesorhizobium loti is not required for nitrogen transfer in the determinate nodules of Lotus corniculatus.
abstract: Deletion of both alanine dehydrogenase genes (aldA) in Mesorhizobium loti resulted in the loss of AldA enzyme activity from cultured bacteria and bacteroids but had no effect on the symbiotic performance of Lotus corniculatus plants. Thus, neither indeterminate pea nodules nor determinate L. corniculatus nodules export alanine as the sole nitrogen secretion product.
authors: School of Animal and Microbial Sciences, University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, United Kingdom.
authors: Shalini
authors: S
authors: Kumar
authors: Alexandre
authors: A
authors: Bourdès
authors: Philip
authors: P
authors: Poole
mesh_terms: D000409:Alanine / Q000096:biosynthesis*; D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001439:Bacteroides / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D017353:Gene Deletion; D005798:Genes, Bacterial; D000070116:Lotus / Q000378:metabolism* / Q000382:microbiology; D009584:Nitrogen / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D003470:Culture Media; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D009584:Nitrogen; D000409:Alanine