Development and application of a new on-line SPE system combined with LC-MS/MS detection for high throughput direct analysis of pharmaceutical compounds in plasma.
A technique using a fully automated on-line solid phase extraction (SPE) system (Symbiosis, Spark Holland) combined with liquid chromatography (LC)-mass spectrometry (MS/MS) has been investigated for fast bioanalytical method development, method validation and sample analysis using both conventional C18 and monolithic columns. Online SPE LC-MS/MS methods were developed in the automated mode for the quantification of model compounds (propranolol and diclofenac) directly in rat plasma. Accuracy and precision using online SPE LC-MS/MS with conventional C18 and monolithic columns were in the range of 88-111% and 0.5-14%, respectively. Total analysis cycle time of 4 min per sample was demonstrated using the C18 column. Monolithic column allowed for 2 min total cycle time without compromising the quality and validation criteria of the method. Direct plasma sample injection without on-line SPE resulted in poor accuracy and precision in the range of 41-108% and 3-81%. Furthermore, the increase in back pressure resulted in column damage after the injection of only 60 samples.
Alnouti
Yazen
Y
Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Eastern Point RD MS 4022, Groton, CT 06340, USA.
Srinivasan
Karthik
K
Waddell
David
D
Bi
Honggang
H
Kavetskaia
Olga
O
Gusev
Arkady I
AI
D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004008:Diclofenac / Q000097:blood; D007052:Ibuprofen / Q000097:blood; D007654:Ketoconazole / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D009862:Online Systems; D004364:Pharmaceutical Preparations / Q000097:blood*; D011433:Propranolol / Q000097:blood; D015203:Reproducibility of Results
D004364:Pharmaceutical Preparations; D004008:Diclofenac; D011433:Propranolol; D007654:Ketoconazole; D007052:Ibuprofen