Fast "hyperlayer" separation development in sedimentation field flow fractionation.
Specific prototypes of sedimentation field flow fractionation devices (SdFFF) have been developed with relative success for cell sorting. However, no data are available to compare these apparatus with commercial ones. In order to compare with other devices mainly used for non-biological species, biocompatible systems were used for standard particle (latex: 3-10 microm of different size dispersities) separation development. In order to enhance size dependent separations, channels of reduced thickness were used (80 and 100 microm) and channel/carrier-phase equilibration procedures were necessary. For sample injection, the use of inlet tubing linked to the FFF accumulation wall, common for cell sorting, can be extended to latex species when they are eluted in the Steric Hyperlayer elution mode. It avoids any primary relaxation steps (stop flow injection procedure) simplifying series of elution processing. Mixtures composed of four different monodispersed latex beads can be eluted in 6 min with 100 microm channel thickness.
Kassab
James R
JR
Laboratoire de Chimie Analytique et de Bromatologie, Université de Limoges, Faculté de Pharmacie, 2 Rue du Dr. Marcland, F-87025 Limoges, Cedex, France.
Cardot
Philippe J P
PJ
Zahoransky
Richard A
RA
Battu
Serge
S
D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D008863:Microspheres; D015203:Reproducibility of Results; D012212:Rheology