Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments.
Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial; mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-alpha-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-alpha-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-alpha-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-alpha-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.
Yuasa
Tetsuya
T
Department of Oral and Maxillofacial Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima, 770-8504, Japan. yuasa@dent.tokushima-u.ac.jp
Miyamoto
Youji
Y
Kon
Masayuki
M
Ishikawa
Kunio
K
Takechi
Masaaki
M
Momota
Yukihiro
Y
Tatehara
Seiko
S
Takano
Hideyuki
H
Mimamiguchi
Shiho
S
Nagayama
Masaru
M
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000704:Analysis of Variance; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D024042:Collagen Type I / Q000096:biosynthesis; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; D012333:RNA, Messenger; D015675:Osteocalcin; D017886:Durapatite; C020243:calcium phosphate; D005993:Glycerolphosphate Dehydrogenase; D000469:Alkaline Phosphatase