Toxicity of indocyanine green (ICG) in combination with light on retinal pigment epithelial cells and neurosensory retinal cells.
OBJECTIVE
To evaluate the toxicity of indocyanine green (ICG) in combination with light.


METHODS
Human retinal pigment epithelial cells (ARPE-19) and rat neurosensory retinal cells (R28) were treated with four different concentrations of ICG in combination with light exposure. Cell viability, mitochondrial function, and DNA synthesis were measured.


RESULTS
All concentrations of ICG with 10 min of light exposure caused a significant decrease in mitochondrial dehydrogenase activity in R28 and ARPE-19 cells. ICG without light exposure did not decrease mitochondrial dehydrogenase activity. In both cell lines, [(3)H]thymidine incorporation was increased when treated with ICG with or without light. R28 cells did not show any significant decrease in cell viability.


CONCLUSIONS
The duration of light was a significant factor in ICG toxicity. ICG needs to be used with caution as it decreases the mitochondrial dehydrogenase activity and increases the DNA synthesis in retinal cells, markers for cell toxicity and dysfunction.
Narayanan
Raja
R
Department of Ophthalmology, University of California-Irvine, 118 Med Surge I, Irvine, CA 92697-4375, USA.
Kenney
M Cristina
MC
Kamjoo
Sami
S
Trinh
Thuan-Hau T
TH
Seigel
Gail M
GM
Resende
Gilberto P
GP
Kuppermann
Baruch D
BD
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004396:Coloring Agents / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007208:Indocyanine Green / Q000008:administration & dosage / Q000633:toxicity*; D008027:Light; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D004396:Coloring Agents; D010088:Oxidoreductases; D007208:Indocyanine Green; D013936:Thymidine