title: Plasmodium falciparum genotyping by microsatellites as a method to distinguish between recrudescent and new infections.
abstract: In vivo tests for susceptibility to antimalarial drugs require molecular methods to distinguish recrudescence from new infection. The most commonly used DNA markers (merozoite surface proteins [MSPs]) are under immune selective pressure, which might lead to misclassification. We evaluated immunologically neutral microsatellite markers in blood samples collected during a drug efficacy trial in Rwanda. Fifty percent of the infections classified as recrudescent by MSP were classified as new by microsatellite markers. Reciprocally, 23.3% of infections classified as recrudescent by microsatellite markers were identified as new by MSP. In drug efficacy studies, microsatellite markers should complement MSP genotyping to distinguish a recrudescence from a new infection.
authors: Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium.
authors: Atunga
authors: A
authors: Nyachieo
authors: Chantal
authors: C
authors: VAN Overmeir
authors: Thierry
authors: T
authors: Laurent
authors: Jean-Claude
authors: JC
authors: Dujardin
authors: Umberto
authors: U
authors: D'Alessandro
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use; D002675:Child, Preschool; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000188:drug therapy; D018895:Microsatellite Repeats; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012008:Recurrence; D017211:Treatment Failure; D016896:Treatment Outcome
chemical_list: D000962:Antimalarials; D005819:Genetic Markers