Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction.
Heterosis was defined as the advantage of hybrid performance over its parents in terms of growth and productivity. Previous studies showed that differential gene expression between hybrids and their parents is responsible for the heterosis; however, information on systematic identification and characterization of the differentially expressed genes are limited. In this study, an interspecific hybrid between common wheat (Triticum aestivum. L., 2n = 6x = 42, AABBDD) line 3338 and spelt (Triticum spelta L. 2n = 6x = 42, AABBDD) line 2463 was found to be highly heterotic in both aerial growth and root related traits, and was then used for expression assay. A modified suppression subtractive hybridization (SSH) was used to generate four subtracted cDNA libraries, and 748 nonreduandant cDNAs were obtained, among which 465 had high sequence similarity to the GenBank entries and represent diverse of functional categories, such as metabolism, cell growth and maintenance, signal transduction, photosynthesis, response to stress, transcription regulation and others. The expression patterns of 68.2% SSH-derived cDNAs were confirmed by reverse Northern blot, and semi-quantitative RT-PCR exhibited the similar results (72.2%). And it was concluded that the genes differentially expressed between hybrids and their parents involved in diverse physiological process pathway, which might be responsible for the observed heterosis.
Yao
Yingyin
Y
Department of Plant Genetics and Breeding, Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing, China Agricultural University, 100094 Beijing, China.
Ni
Zhongfu
Z
Zhang
Yinhong
Y
Chen
Yan
Y
Ding
Yuhua
Y
Han
Zongfu
Z
Liu
Zhiyong
Z
Sun
Qixin
Q
D015152:Blotting, Northern; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D006823:Hybrid Vigor / Q000235:genetics; D006824:Hybridization, Genetic / Q000235:genetics; D007178:Inbreeding; D009693:Nucleic Acid Hybridization / Q000379:methods; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
D010940:Plant Proteins