title: The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells.
abstract: Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aalpha, Bbeta, and gamma, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.
authors: Institute of Molecular and Cell Biology, Proteos, Singapore. mcbtanyj@imcb.a-star.edu.sg
authors: Yee-Joo
authors: YJ
authors: Tan
authors: Puay-Yoke
authors: PY
authors: Tham
authors: Daphne Z L
authors: DZ
authors: Chan
authors: Chih-Fong
authors: CF
authors: Chou
authors: Shuo
authors: S
authors: Shen
authors: Burtram C
authors: BC
authors: Fielding
authors: Timothy H P
authors: TH
authors: Tan
authors: Seng Gee
authors: SG
authors: Lim
authors: Wanjin
authors: W
authors: Hong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D005340:Fibrinogen / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D045473:Severe acute respiratory syndrome-related coronavirus; D015854:Up-Regulation; D014709:Vero Cells; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: C487105:3a protein, SARS-CoV; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000086082:Viroporin Proteins; D005340:Fibrinogen