[Studies of immunoaffinity chromatography for carbofuran].
The purified anti-carbofuran antibody was conjugated to carbonyl diimidazole (CDI)-activated Sepharose CL-4B to synthesize the immunosorbent for the immunoaffinity chromatographic (IAC) column specific to carbofuran. The conditions of IAC were optimized as follows: pH 7.2 phosphate buffer (PB) was used as equilibrium and adsorbent medium, and methanol-water (60:40, v/v) as eluent. The results showed that the dynamic column capacity was up to 1.58 mg/L bed volume. The efficiency of enrichment of IAC was more than 167 times when the initial concentration of carbofuran in a standard sample solution was lower than 2 microg/L. The spiked river water was cleaned up and enriched by IAC, and carbofuran in eluate was determined by enzyme linked immunosorbent assay (ELISA). The average recovery of carbofuran from river water was 89.8% with the relative standard deviation of 4.8% at the spiked level of 0.1 mg/L. Meanwhile the eluate was determined by high performance liquid chromatography (HPLC), the results from HPLC correlated well with those from ELISA. The IAC method of carbofuran was successfully established.
Liu
Shuzhao
S
College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, China. szliu@yzu.edu.cn
Wei
Linhong
L
Xu
Weina
W
D000906:Antibodies / Q000032:analysis*; D002235:Carbofuran / Q000032:analysis* / Q000276:immunology*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007120:Immunochemistry / Q000295:instrumentation / Q000379:methods; D007306:Insecticides / Q000032:analysis* / Q000276:immunology*; D015394:Molecular Structure; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods
D000906:Antibodies; D007306:Insecticides; D002235:Carbofuran