title: [Comparison of two amine-modified chemical platforms for DNA microarray preparation].
abstract: OBJECTIVE
  To study two amine modification procedures for DNA microarray preparation based on polymeric coatings.
  
  
  METHODS
  One of the proposed approaches utilized poly-amine coating of silanized slides activated by 1,4-phenylene diisothiocyanate, and the other employed acrylic acid-co-acrylamide copolymer and 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide as the coating agents and activator, respectively. The modified slides were used for preparing lambda phage DNA microarrays, whose properties were analyzed by hybridization.
  
  
  RESULTS
  Formation of dendrimeric structure and polymer was observed on the surface of the slides. The signal spots in uniform, steady and regular shape, in comparison with the commercial CMT-GAPS slides, indicated successful manufacture of the microarrays.
  
  
  CONCLUSIONS
  The two platforms are suitable for microarray preparation, and the method of acrylic acid-co-acrylamide copolymer modification is more preferred.
authors: Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. wuqh@fimmu.com
authors: Qing-hua
authors: QH
authors: Wu
authors: Wen-li
authors: WL
authors: Ma
authors: Hong-min
authors: HM
authors: Wang
authors: Xiang-ming
authors: XM
authors: Mao
authors: Bao
authors: B
authors: Zhang
authors: Ling
authors: L
authors: Li
authors: Wen-ling
authors: WL
authors: Zheng
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000588:Amines / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011108:Polymers / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000588:Amines; D011108:Polymers; C431184:poly(acrylamide-co-acrylic acid)