title: The effect of estradiol and progesterone application on leptin concentration in ovariectomized rats.
abstract: The aim of the present study was to investigate the effects of estradiol and progesterone application on leptin secretion in ovariectomised rats. The study included 30 adult female Sprague-Dawley rats. Rats were divided into three groups as follows: Group 1; Sham ovariectomy group (n=10), Group 2; Ovariectomy group (n=10), Group 3; Ovariectomized and estradiol propionate (450 microg/kg rat) and medroxyprogesterone acetate (15 mg/kg rat) supplemented group (n=10). One week after ovariectomy, rats in Group 3 were injected estradiol and progesterone for 4 weeks. Twenty-four hours after the last injection, rats were decapitated and blood samples were collected for leptin analysis. Serum leptin levels in Group 2 were found significantly higher than those in Groups 1 and 3 (p<0.01), while those in Group 3 were significantly lower when compared to Group 1 (p<0.0 1). The findings of the present study have shown that ovariectomy led to a significant increase in leptin levels. However, administration of estradiol and progesterone in combination following ovariectomy inhibites increases of leptin levels.
authors: Department of Biochemistry, Meram Medical School, Selcuk University, Konya, Turkey. eoztekin@selcuk.edu.tr
authors: E
authors: E
authors: Oztekin
authors: R
authors: R
authors: Mogulkoc
authors: A K
authors: AK
authors: Baltaci
authors: A M
authors: AM
authors: Tiftik
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D020738:Leptin / Q000097:blood*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020738:Leptin; D004958:Estradiol; D017258:Medroxyprogesterone Acetate