A comparison between in vivo and ex vivo HR-MAS 1H MR spectra of a pediatric posterior fossa lesion.
The present case report was aimed at identifying the molecular profile characteristic of a primitive neuro-ectodermal tumor (PNET) in a 3-year-old child affected by a lesion localized in the cerebellar region. The histological diagnosis was medulloblastoma. In vivo single voxel 1H magnetic resonance spectroscopy (MRS) shows high specificity in detecting the main metabolic alterations in the primitive cerebellar lesion; a very high amount of the choline-containing compounds and very low level of creatine derivatives and N-acetylaspartate. Ex vivo high resolution magic angle spinning (HR-MAS) 1H magnetic resonance spectroscopy, performed at 9.4 Tesla on the neoplastic specimen collected during surgery, allows for the unambiguous identification of several metabolites giving a more in-depth evaluation of the metabolic pattern of the lesion. The ex vivo HR-MAS MR spectra show that the spectral detail is much higher than that obtained in vivo and that, for example, myo-inositol, taurine and phosphorylethanolamine contribute to the in vivo signal at 3.2 ppm, usually attributed to choline-containing compounds. In addition, the spectroscopic data appear to correlate with some morphological features of the medulloblastoma. Consequently, the present study shows that ex vivo HR-MAS 1H MRS is able to strongly improve the clinical possibility of in vivo MRS and can be used in conjunction with in vivo spectroscopy for clinical purposes.
Tugnoli
V
V
Dipartimento di Biochimica, Università di Bologna, via Belmeloro 8/2, 40126 Bologna, Italy. vitaliano.tugnoli@unibo.it
Schenetti
L
L
Mucci
A
A
Nocetti
L
L
Toraci
C
C
Mavilla
L
L
Basso
G
G
Rovati
R
R
Tavani
F
F
Zunarelli
E
E
Righi
V
V
Tosi
M R
MR
D019002:CD56 Antigen / Q000032:analysis; D002528:Cerebellar Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D002675:Child, Preschool; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008527:Medulloblastoma / Q000175:diagnosis* / Q000378:metabolism; D018242:Neuroectodermal Tumors, Primitive / Q000175:diagnosis* / Q000378:metabolism
D019002:CD56 Antigen