[The diagnostic significance of Ig heavy chain rearrangement detected in B cell lymphoma patients' serum or plasma blood samples].
OBJECTIVE
To evaluate the diagnostic significance of detecting immunoglobulin (Ig) heavy chain (IgH) by using serum or plasma as blood samples.


METHODS
First, collect serum and plasma blood samples of patients with B-NHL and extract tumor-derived DNAs. Then design the primer to amplify framework3 (Fr3) from the V segment regions to the J regions of the IgH complementary determining region III (CDR-III) gene. Detect the positive ratio of IgH rearrangement with PCR and evaluate its diagnostic significance.


RESULTS
B lymphoma cell line Raji was used as the positive control. Of the 30 B-NHL cases diagnosed with morphologic study, 25 (83.3%) showed IgH rearrangement. DNAs extracted from healthy adults and chronic lymphadenitis patients showed negative result. There was no statistical pertinence between IgH gene rearrangement and clinical manifestation, clinical staging and tumor burden.


CONCLUSIONS
Tumor-derived DNA can be detected in serum or plasma of the majority of patients with B-cell lymphoma. Testing of serum or plasma for tumor associated DNA may be a novel parameter for clinical diagnosis without the restriction of the position of enlarged lymph node.
Feng
Qi
Q
The Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China. iamfengqi@263.net
Li
Hong-ling
HL
Sun
Kai
K
Su
Ming-quan
MQ
Shan
Zhen-chuan
ZC
Sun
Bing-zhong
BZ
D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000097:blood; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains