Gene expression changes induced by a recombinant E1-/E3- adenovirus type 5 vector in human mammary epithelial cells.
OBJECTIVE
Adenoviral vectors are used in transferring exogenous genes to a variety of cells and tissue types both in vitro and in vivo. Gene expression changes induced by an E1/E3-defective adenovirus vector have been studied in human mammary epithelial cells by comparing the gene expression profile in infected and uninfected cells.


METHODS
The human mammary epithelial cell line HB2 was infected with an E1/E3-defective adenovirus type 5 vector. Total RNA was extracted from infected and uninfected cells 24 and 72 h after infection and subjected to microarray analysis using the Affymetrix U133A genomic chip system. Semiquantitative RT-PCR confirmed the regulation of genes observed by microarray analysis.


RESULTS
The microarray analysis showed 24 and 95 transcripts to be regulated 24 and 72 h after infection, respectively. A relatively high number of genes involved in innate and inflammatory host immune responses, including interleukin-8, interleukin-6, NF-kappaB(2), RELB and fos, were induced. As expected from an E1-defective virus, changes in the expression of genes involved in the G1-S transition and in the activation of cell proliferation were not detected.


CONCLUSIONS
Our study provides insight into the host transcriptional response following transduction of an adenoviral vector into mammary epithelial cells.
Scibetta
Angelo G
AG
Cancer Research UK, Breast Cancer Biology Group, Guys Hospital, London, UK. angelo.scibetta@cancer.org.uk
Copier
John
J
Barrett
Angela
A
Chaplin
Tracy
T
Taylor-Papadimitriou
Joyce
J
D017498:Adenovirus E1 Proteins / Q000235:genetics; D017503:Adenovirus E3 Proteins / Q000235:genetics; D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D003673:Defective Viruses / Q000235:genetics; D004847:Epithelial Cells / Q000502:physiology* / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology* / Q000502:physiology / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017498:Adenovirus E1 Proteins; D017503:Adenovirus E3 Proteins