title: Optimal recovery of high-purity rutin crystals from the whole plant of Fagopyrum esculentum Moench (buckwheat) by extraction, fractionation, and recrystallization.
abstract: Rutin, one of the flavonoids derived from plants, is increasingly in demand in the food, pharmaceutical, and cosmetic industries due to its various biological and physiological activities including antioxidation, anti-inflammation, and anti-hypertension. The whole plant of buckwheat (Fagopyrum esculentum Moench) is a major source of natural rutin. This study developed a low-cost process encompassing the efficient extraction, fractionation, and recrystallization to obtain high-purity rutin from buckwheat, and it could improve the economic utilization of this abundant low-value agricultural product. The sequential separation and purification procedures established in this study involved extraction with 50% (v/v) aqueous ethanol at 80 degrees C for 1 h followed by elution with water and aqueous ethanols at 20% and 30% (v/v) on a styrene-based resin column, and recrystallization at 4 degrees C for 12 h. These conditions resulted in the recovery of 92% of total rutin with over 95% purity. In the present study, high-purity rutin was obtained from whole buckwheat through low-cost processes, the separation and purification strategy established in this study could provide valuable information to the relevant industries.
authors: Division of Food Science, College of Life and Environmental Sciences, Korea University, Seoul 136-713, Republic of Korea. khekim@korea.ac.kr
authors: Kyoung Heon
authors: KH
authors: Kim
authors: Ki Won
authors: KW
authors: Lee
authors: Dong Young
authors: DY
authors: Kim
authors: Hyung Hwan
authors: HH
authors: Park
authors: Ik Boo
authors: IB
authors: Kwon
authors: Hyong Joo
authors: HJ
authors: Lee
mesh_terms: D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D000431:Ethanol; D019612:Fagopyrum / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification; D012431:Rutin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D000431:Ethanol; D012431:Rutin