Identification of broad specificity P450CAM variants by primary screening against indole as substrate.
High-throughput screening of cytochrome P450CAM libraries, for their ability to oxidise indole to indigo and indirubin, has resulted in the identification of variants with activity towards the structurally unrelated substrate diphenylmethane.
Celik
Ayhan
A
School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, UKEH9 3JJ. n.j.turner@ed.ac.uk
Speight
Robert E
RE
Turner
Nicholas J
NJ
D019475:Camphor 5-Monooxygenase / Q000378:metabolism*; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D013379:Substrate Specificity
D007211:Indoles; C030374:indole; D019475:Camphor 5-Monooxygenase