title: Two-dimensional electrophoresis database of fluorescence-labeled proteins of colon cancer cells.
abstract: We constructed a novel database of the proteome of DLD-1 colon cancer cells by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of fluorescence-labeled proteins followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis. The database consists of 258 functionally categorized proteins corresponding to 314 protein spots. The majority of the proteins are oxidoreductases, cytoskeletal proteins and nucleic acid binding proteins. Phosphatase treatment showed that 28% of the protein spots on the gel are phosphorylated, and mass spectrometric analysis identified 21 of them. Proteins of DLD-1 cells and of laser-microdissected colon cancer tissues showed similar distribution on 2D gels, suggesting the utility of our database for clinical proteomics.
authors: Cancer Proteomics Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Tokyo 104-0045, Japan.
authors: Yasuharu
authors: Y
authors: Mori
authors: Tadashi
authors: T
authors: Kondo
authors: Tesshi
authors: T
authors: Yamada
authors: Akihiko
authors: A
authors: Tsuchida
authors: Tetsuya
authors: T
authors: Aoki
authors: Setsuo
authors: S
authors: Hirohashi
mesh_terms: D000595:Amino Acid Sequence; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002232:Carbocyanines; D010750:Phosphoproteins; D011506:Proteins; C089187:cyanine dye 3; C085321:cyanine dye 5; D010744:Phosphoric Monoester Hydrolases