title: Analysis of poly(amidoamine)-succinamic acid dendrimers by slab-gel electrophoresis and capillary zone electrophoresis.
abstract: Ethylenediamine (EDA)-core poly(amidoamine) (PAMAM) succinamic acid dendrimers (Ex.SAH, where x refers to the generation) were synthesized and analyzed by polyacrylamide gel electrophoresis (PAGE), size-exclusion chromatography (SEC), potentiometric acid-base titration, and capillary zone electrophoresis (CZE). Various generations (E1.SAH-E7.SAH) PAMAMs and a succinamic acid terminated core-shell tecto(dendrimer) (E5(E3.SAH)(n)) were first analyzed by PAGE. PAGE results show that the relative mobilities of generation 2 to generation 7 dendrimers decreased with the increasing number of generations. The molecular mass of a generation 5 core generation 3 shell tecto(dendrimer) (denoted as E5(E3.SAH)(n)) was determined to be between the Mw of E6.SAH and E7.SAH. CZE analysis allowed the evaluation of electrophoretic properties of given-generation dendrimers. The electrophoretic mobilities of individual generations PAMAM polyanions are similar, indicating that the separation mainly depends on their approximately identical charge/mass ratio. The E5(E3.SAH)(n) tectodendrimer had a lower electrophoretic mobility, which was consistent with its lower charge/mass ratio. The combination of PAGE and CZE analysis provides an alternative and effective way to characterize this group of PAMAM-succinamic acid dendrimers.
authors: Center for Biologic Nanotechnology, University of Michigan Medical School, Ann Arbor, USA.
authors: Xiangyang
authors: X
authors: Shi
authors: Anil K
authors: AK
authors: Patri
authors: Wojciech
authors: W
authors: Lesniak
authors: Mohammad T
authors: MT
authors: Islam
authors: Chunxin
authors: C
authors: Zhang
authors: James R
authors: JR
authors: Baker
authors: Lajos P
authors: LP
authors: Balogh
mesh_terms: D050091:Dendrimers / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D011073:Polyamines / Q000032:analysis*; D013386:Succinates / Q000032:analysis* / Q000737:chemistry
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D013386:Succinates