title: Pilot field trial of a recombinant Taenia ovis vaccine in lambs exposed to natural infection.
abstract: Previous trials of an experimental Taenia ovis vaccine using the recombinant antigen GST--45W(B/X) established that it was possible to achieve >90% protection against a single artificial challenge of T. ovis eggs. This trial was undertaken to assess vaccine efficacy against artificial challenge and natural infection acquired by lambs grazing contaminated pasture. Two hundred Romney lambs were vaccinated at 6 and 12 weeks of age. One hundred control lambs were not vaccinated but were allowed to run with the vaccinated mob. At 15 weeks of age, 10 controls and 18 vaccinated lambs were artificially challenged with 2000 T. ovis eggs. The remaining control and vaccinated lambs were allowed to graze contaminated pasture for 3 weeks and were then moved to clean pasture for 5 months. The artificially challenged lambs plus 24 of the field-infected lambs were slaughtered and the carcasses dissected to obtain cyst counts. The remaining field-infected lambs were slaughtered at a commercial processing plant and the carcasses examined by conventional meat inspection. The results showed that the vaccine provided a high level of protection against artificial challenge (92%) and natural infection (98%) when assessed by carcass dissection. The data from commercial meat inspection showed that vaccination provided 89% efficacy against downgrading or condemnation compared to non-vaccinated control lambs. The average difference in carcass values between vaccinated and non-vaccinated groups was 4.36 dollars, representing a 35% loss in value due to T.ovis infection in non-vaccinated lambs.
authors: AgResearch, Wallaceville Animal Research Centre, PO Box 40-063, Upper Hutt, New Zealand.
authors: S B
authors: SB
authors: Lawrence
authors: D D
authors: DD
authors: Heath
authors: G B
authors: GB
authors: Harrison
authors: C M
authors: CM
authors: Robinson
authors: R P
authors: RP
authors: Dempster
authors: T K
authors: TK
authors: Gatehouse
authors: M W
authors: MW
authors: Lightowlers
authors: M D
authors: MD
authors: Rickard