Staphylococcal Drp35 is the functional counterpart of the eukaryotic PONs.
Drp35 has been identified as a protein that is induced in Staphylococcus aureus in response to exposure to certain antibiotics. Here we demonstrate that Drp35 is a lactonase that does not contribute directly to the resistance to the inducer antibiotics except for bacitracin. The detailed analysis on the expression of Drp35 revealed that in addition to a broad range of antibiotics, agents such as detergents that perturb the membrane integrity could induce its expression. The significance of this characteristic expression is discussed in relation to its activity similarity to the eukaryotic counterparts, paraoxonase family proteins.
Morikawa
Kazuya
K
Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennoh-dai, Tsukuba 305-8575, Japan. morikawa@sakura.cc.tsukuba.ac.jp
Hidaka
Toshie
T
Murakami
Hiroyuki
H
Hayashi
Hideo
H
Ohta
Toshiko
T
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D001414:Bacitracin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D005057:Eukaryotic Cells / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial; D006867:Hydrolases; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; C400364:drp35 protein, Staphylococcus aureus; D001414:Bacitracin; D006867:Hydrolases; D043303:Aryldialkylphosphatase