Triplet excited States as chiral reporters for the binding of drugs to transport proteins.
The triplet excited state of flurbiprofen methyl ester (FBPMe) has been used as a chiral reporter for the two binding sites of human serum albumin (HSA). The occupation level of the binding sites has been estimated from regression analysis of the triplet decays at several [FBPMe]/[HSA] ratios. The data agree with two high affinity binding sites (I and II) that are populated to a different extent. A remarkable stereodifferentiation has been found in the drug triplet lifetimes within the protein microenvironment.
Jiménez
M Consuelo
MC
Departamento de Química, Instituto de Tecnología Química UPV-CSIC, Universidad Politécnica de Valencia, camino de Vera s/n, E-46022 Valencia, Spain.
Miranda
Miguel A
MA
Vayá
Ignacio
I
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D005480:Flurbiprofen / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D006801:Humans; D010782:Photolysis; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002352:Carrier Proteins; D012709:Serum Albumin; D005480:Flurbiprofen