Assays for membrane tyrosine kinase receptors: methods for high-throughput screening and utility for diagnostics.
The development of novel antagonists or agonists of membrane tyrosine kinase receptors is a large focus of discovery research. This review will provide some background on membrane tyrosine kinases as well as a description of some of the better established assays used for the high-throughput screening of membrane tyrosine kinase inhibitors. Biochemical methods detailed include those using labels such as radioactivity and fluorescence (fluorescence energy transfer, fluorescence and fluorescence polarization) as well as label-free assays using luminescence. These assays are solid phase, liquid phase, as well as bead based. In addition, a discussion on which tools are available to screen for membrane tyrosine kinase receptor modulators/activators using whole-cell assays will be presented. The potential clinical need for testing receptor activation/phosphorylation as well as the possibility of using some of these tests to measure biomarkers of disease or as clinical diagnostic tools to tailor drug therapy or monitor its efficacy will also be discussed.
Minor
Lisa K
LK
Johnson & Johnson Pharmaceutical Research and Development, LLC, Springhouse Pennsylvania, Welsh & McKean Rds, Springhouse, PA 19477, USA. lminor@prdus.jnj.com
D000818:Animals; D016503:Drug Delivery Systems; D005453:Fluorescence; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021381:Protein Transport; D011868:Radioisotopes; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis* / Q000378:metabolism*
D011868:Radioisotopes; D020794:Receptor Protein-Tyrosine Kinases