Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells.
We have synthesized a biologically inert, photosensitive derivative of the major excitatory amino acid, L-glutamate (which we call MDNI-glu) that makes more efficient use of incident light than all other caged glutamates. Laser flash photolysis of MDNI-glu in acutely isolated hippocampal brain slices evoked a rapid increase in intracellular Ca2+ concentration in astrocytes.
Fedoryak
Olesya D
OD
Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
Sul
Jai-Yoon
JY
Haydon
Philip G
PG
Ellis-Davies
Graham C R
GC
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D005971:Glutamates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010782:Photolysis / Q000528:radiation effects; D017785:Photons; D013057:Spectrum Analysis
D005971:Glutamates; D002118:Calcium