A genetically engineered strain of Pseudomonas putida as a useful tool for identifying new therapeutic herbicides.
A genetically engineered strain of Pseudomonas putida U designed for the identification of new therapeutic herbicides has been obtained. In this bacterium, deletion of the homogentisate gene cluster (hmgRABC) confers upon this mutant huge biotechnological possibilities since it can be used: (i) as a target for testing new specific herbicides (p-hydroxy-phenylpyruvate dioxygenase inhibitors); (ii) to identify new therapeutic drugs-effective in the treatment of alkaptonuria and other related tyrosinemia - and (iii) as a source of homogentisic acid in a plant-bacterium association.
Arias-Barrau
Elsa
E
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, España, Spain.
Sandoval
Angel
A
Arcos
Mario
M
Arias
Sagrario
S
Naharro
Germán
G
Olivera
Elías R
ER
Luengo
José M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005818:Genetic Engineering / Q000379:methods; D006540:Herbicides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D016958:Pseudomonas putida / Q000187:drug effects / Q000235:genetics*; D015183:Restriction Mapping
D017931:DNA Primers; D006540:Herbicides