[Determination of urinary NAG to detect renal ischemia-reperfusion injury and the protective effect of Allopurinol].
We analysed the effect of ischemia-reperfusion injury to renal parenchyma after unilateral renal artery clamping using urinary N-acetyl-beta-D-glucosaminidase (NAG) that is a sensitive parameter of early renal tubular injury. In the study 60 mongrel dogs were divided into 3 groups: in the 1st group the left renal artery was clamped for 45 minutes, in the 2nd group Allopurinol was administered before the clamping, the 3rd was the control group, where only laparotomy and closure of the abdomen was performed. Urinary NAG activity referring to urinary creatinine (NAG index) was determined before the operation, at the beginning of the reperfusion, in the 60th and 120th minute of the reperfusion then 1, 2, 3 and 5 days after the operation. The highest NAG indices relating to injury of the proximal tubuli were found at the beginning of the reperfusion, in the 60th and 120th minutes of the reperfusion, then NAG returned to preoperative level in each group. Significantly higher NAG indices were found in the ischemia-reperfusion group compared to the group with Allopurinol pretreatment. Renal ischemia-reperfusion injury and the protective effect of Allopurinol could be detected by lysosomal NAG enzyme. The injury of the tubular function was reversible so it could be a change in tubular function.
Peto
Katalin
K
Debreceni Egyetem Orvos- és Egészségtudományi Centrum, Sebészeti Mutéttani Tanszék. kpeto@jaguar.unideb.hu
Oláh
V Anna
VA
Bráth
Endre
E
Németh
Norbert
N
Gulyás
Adrienn
A
Szilasi
Mária
M
Sárvári
Mónika
M
Furka
István
I
Mikó
Irén
I
D000118:Acetylglucosaminidase / Q000652:urine*; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D003404:Creatinine / Q000652:urine; D004285:Dogs; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007684:Kidney Tubules / Q000201:enzymology / Q000293:injuries / Q000378:metabolism*; D015427:Reperfusion Injury / Q000175:diagnosis* / Q000201:enzymology / Q000652:urine; D013997:Time Factors
D004791:Enzyme Inhibitors; D000493:Allopurinol; D003404:Creatinine; D000118:Acetylglucosaminidase