title: Neuropeptide Y2 receptor protein is present in peptidergic and nonpeptidergic primary sensory neurons of the mouse.
abstract: The localization of the neuropeptide tyrosine (NPY) Y2 receptor (Y2R) protein was studied in mouse dorsal root ganglia (DRGs) and spinal cord, by using a recently developed rabbit anti-Y2R antibody and a sensitive immunohistochemical method. Y2R-like immunoreactivity (-LI) was observed in about 10% of the small/medium-sized lumbar DRG neurons. Among these, about 44% were calcitonin gene-related peptide-immunoreactive, and about 38% bound isolectin B4. In the dorsal horn of the spinal cord, an intense Y2R-LI was seen in the most superficial layers, mostly restricted to laminae I-II. This immunoreactivity was completely abolished by dorsal rhizotomy. Y2R-L1 was also detected on the skin, more abundantly in hairy than glabrous skin. Specificity experiments showed complete disappearance of the Y2R-LI described above after incubation with antibody preadsorbed with the immunogenic peptide. Furthermore, Y2R-LI was also absent in a Y2R knockout mouse. These results demonstrate that the NPY Y2R is associated mainly with both peptidergic and nonpeptidergic small, presumably nociceptive, neurons projecting to the superficial layers of the dorsal horn. The results also support a role for this receptor and NPY in pain mechanisms.
authors: Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden. pablo.brumovsky@neuro.ki.se
authors: Pablo
authors: P
authors: Brumovsky
authors: Davor
authors: D
authors: Stanic
authors: Sam
authors: S
authors: Shuster
authors: Herbert
authors: H
authors: Herzog
authors: Marcelo
authors: M
authors: Villar
authors: Tomas
authors: T
authors: Hökfelt
mesh_terms: D000818:Animals; D019771:Axotomy / Q000379:methods; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009409:Nerve Crush / Q000379:methods; D009475:Neurons, Afferent / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000172:deficiency / Q000378:metabolism*; D019051:Rhizotomy / Q000379:methods; D013116:Spinal Cord / Q000166:cytology*; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: C045475:Griffonia simplicifolia lectins; D009479:Neuropeptides; D037121:Plant Lectins; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse; D015740:Calcitonin Gene-Related Peptide