title: [Study on the relationship between cytosolic phospholipase A2-gamma activation and myocardial cell injury during cardiopulmonary bypass].
abstract: OBJECTIVE
  To investigate the relationship between cytosolic phospholipase A(2)-gamma(cPLA(2)-gamma) activation and alteration of myocardial ultrastructure during cardiopulmonary bypass (CPB) in the operation of ventricular septal defect.
  
  
  METHODS
  Myocardial tissues from the right atria of 12 patients undergone ventricular septal defect were collected before and after CPB, cPLA(2)-gamma gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and analysis was carried out using gel image analysis software. Meanwhile, alteration of myocardial ultrastructure was observed under electron microscopy.
  
  
  RESULTS
  cPLA(2)-gamma gene expressions were statistically significant between two groups (P<0.05), and they were higher in patients after CPB than those prior to CPB. The alteration of myocardial ultrastructure after CPB included hypertrophy of nucleus, swelling under plasma membrane, chromatin margination and dilatation of smooth endoplasmic reticulum (SER) in myocardial cell, structures degeneration, hyperplasia of mitochondria as well as swelling, and myocardial fibre dissolve, etc.
  
  
  CONCLUSIONS
  Increased cPLA(2)-gamma gene expression in myocardial tissue after CPB might play an important role in damage of membrane integrity, energy decompensation and myocardial contraction dysfunction.
authors: PICU of Children's Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China.
authors: Xiao-yun
authors: XY
authors: Wu
authors: Feng-wu
authors: FW
authors: Kuang
authors: Zi-guo
authors: ZG
authors: Luo
authors: Bing
authors: B
authors: Deng
authors: Chun
authors: C
authors: Wu
authors: Xing-yong
authors: XY
authors: Wang
mesh_terms: D002315:Cardiopulmonary Bypass; D002648:Child; D002675:Child, Preschool; D005260:Female; D054513:Group IV Phospholipases A2 / Q000378:metabolism*; D006345:Heart Septal Defects, Ventricular / Q000601:surgery; D006801:Humans; D007223:Infant; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000648:ultrastructure*
chemical_list: D054513:Group IV Phospholipases A2