Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping.
Accurate and comprehensive sequence coverage for large genomes has been restricted to only a few species of specific interest. Lower sequence coverage (survey sequencing) of related species can yield a wealth of information about gene content and putative regulatory elements. But survey sequences lack long-range continuity and provide only a fragmented view of a genome. Here we show the usefulness of combining survey sequencing with dense radiation-hybrid (RH) maps for extracting maximum comparative genome information from model organisms. Based on results from the canine system, we propose that from now on all low-pass sequencing projects should be accompanied by a dense, gene-based RH map-construction effort to extract maximum information from the genome with a marginal extra cost.
Hitte
Christophe
C
CNRS, UMR 6061, Génétique et développement, Faculte de Médecine, Rennes, France.
Madeoy
Jennifer
J
Kirkness
Ewen F
EF
Priat
Catherine
C
Lorentzen
Travis D
TD
Senger
Fabrice
F
Thomas
Dan
D
Derrien
Thomas
T
Ramirez
Christina
C
Scott
Carol
C
Evanno
Gwenaelle
G
Pullar
Barbara
B
Cadieu
Edouard
E
Oza
Vinay
V
Lourgant
Kristelle
K
Jaffe
David B
DB
Tacher
Sandrine
S
Dréano
Stéphane
S
Berkova
Nadia
N
André
Catherine
C
Deloukas
Panagiotis
P
Fraser
Claire
C
Lindblad-Toh
Kerstin
K
Ostrander
Elaine A
EA
Galibert
Francis
F
D000818:Animals; D004285:Dogs; D006801:Humans; D010802:Phylogeny; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA