title: The effects of cranberry juice consumption on antioxidant status and biomarkers relating to heart disease and cancer in healthy human volunteers.
abstract: BACKGROUND
  Consumption of fruit and vegetables is associated with a decreased risk of heart disease and cancer. This has been ascribed in part to antioxidants in these foods inactivating reactive oxygen species involved in initiation or progression of these diseases. Non-nutritive anthocyanins are present in significant amounts in the human diet. However, it is unclear whether they have health benefits in humans.
  
  
  OBJECTIVE
  To determine whether daily consumption of anthocyanin-rich cranberry juice could alter plasma antioxidant activity and biomarkers of oxidative stress.
  
  
  METHODS
  20 healthy female volunteers aged 18-40 y were recruited. Subjects consumed 750 ml/day of either cranberry juice or a placebo drink for 2 weeks. Fasted blood and urine samples were obtained over 4 weeks. The total phenol, anthocyanin and catechin content of the supplements and plasma were measured. Anthocyanin glycosides were identified by tandem mass spectrometry (MS-MS). Vitamin C, homocysteine (tHcy) and reduced glutathione (GSH) were measured by HPLC. Total antioxidant ability was determined using electron spin resonance (ESR) spectrometry and by the FRAP assay. Plasma total cholesterol, high density lipoprotein (HDL), and low density lipoprotein (LDL) cholesterol and triglycerides (TG) were measured. Glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities were measured in erythrocytes. Urine was collected for analysis of malondialdehyde (MDA) by HPLC and 8-oxo-deoxyguanosine (8-oxo-dG) by ELISA. Endogenous and induced DNA damage were measured by single cell gel electrophoresis (SCGE) in lymphocytes.
  
  
  RESULTS
  Vitamin C, total phenol, anthocyanin and catechin concentrations and FRAP and ESR values were significantly higher in the cranberry juice compared with the placebo. Cyanidin and peonidin glycosides comprised the major anthocyanin metabolites [peonidin galactoside (29.2%) > cyanidin arabinoside (26.1%) > cyanidin galactoside (21.7%) > peonidin arabinoside (17.5%) > peonidin glucoside (4.1%) > cyanidin glucoside (1.4 %)]. Plasma vitamin C increased significantly (P<0.01) in volunteers consuming cranberry juice. No anthocyanins (plasma) or catechins (plasma or urine) were detectable and plasma total phenols, tHcy,TC,TG,HDL and LDL were unchanged. The antioxidant potential of the plasma, GSH-Px, CAT and SOD activities, and MDA were similar for both groups. Supplementation with cranberry juice did not affect 8-oxo-deoxyguanosine in urine or endogenous or H(2)O(2)-induced DNA damage in lymphocytes.
  
  
  CONCLUSIONS
  Cranberry juice consumption did not alter blood or cellular antioxidant status or several biomarkers of lipid status pertinent to heart disease. Similarly, cranberry juice had no effect on basal or induced oxidative DNA damage. These results show the importance of distinguishing between the in vitro and in vivo antioxidant activities of dietary anthocyanins in relation to human health.
authors: Phytochemicals and Genomic Stability Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen (Sco) AB21 9SB, UK. sd@rri.sari.ac.uk
authors: S J
authors: SJ
authors: Duthie
authors: A McE
authors: AM
authors: Jenkinson
authors: A
authors: A
authors: Crozier
authors: W
authors: W
authors: Mullen
authors: L
authors: L
authors: Pirie
authors: J
authors: J
authors: Kyle
authors: L S
authors: LS
authors: Yap
authors: P
authors: P
authors: Christen
authors: G G
authors: GG
authors: Duthie
mesh_terms: D000293:Adolescent; D000328:Adult; D000872:Anthocyanins / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism*; D001628:Beverages; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D006331:Heart Diseases / Q000453:epidemiology / Q000209:etiology; D006801:Humans; D009369:Neoplasms / Q000453:epidemiology / Q000209:etiology; D018384:Oxidative Stress / Q000187:drug effects*; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers