title: Binding of natural variants of staphylococcal superantigens SEG and SEI to TCR and MHC class II molecule.
abstract: SEG and SEI are staphylococcal superantigens (SAgs) identified recently that belong to the egc operon and whose genes are in tandem orientation. Only a few allelic variants of SEG and SEI have been reported. Here we analyzed four Staphylococcus aureus strains with genotypic variation in both SAgs. However, both SAgs retain key residues in their putative TCR and MHC binding sites and, accordingly, their superantigenic properties. Thus, SEI significantly stimulates mouse T-cells bearing Vbeta3, 5 and 13, while SEG stimulates Vbeta7 and 9 in the draining node when inoculated in the footpad. As another member of the SEB subfamily, SEG also stimulates mouse Vbeta8.1+2. However, the increase in Vbeta8.1+2 T-cells observed at day 2 after inoculation reverts to normal values at day 4, whereas it remains high at day 4 following inoculation with SEC3 or SSA. T-cell stimulation assays in the mouse and analysis of the putative Vbeta8.2 binding site on SEG, which includes three non-conserved residues, suggest a possibly unique interaction between Vbeta8.2 and SEG. We also analyzed biochemical and biophysical characteristics of SEI and SEG binding to their cognate human beta chains by surface plasmon resonance, and binding to the HLA-DR1 MHC class II molecule by gel filtration. SEI binds human Vbeta5.2 and Vbeta1 with apparent K(D)'s of 23 and 118 microM, respectively; SEG binds Vbeta13.6 with a K(D) of 5 microM. As suggested by sequence homology, SEI requires Zn2+ for strong binding to DR1, which goes undetected in the presence of EDTA. SEG and SEI have characteristics such as co-expression, different interaction with MHC class II and stimulation of completely different subsets of human and mouse T-cells, which indicate complementary superantigenic activity and suggest an important advantage to staphylococcal strains in producing them both.
authors: Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors: Marisa M
authors: MM
authors: Fernández
authors: Mauricio C
authors: MC
authors: De Marzi
authors: Paula
authors: P
authors: Berguer
authors: Dalia
authors: D
authors: Burzyn
authors: Ries J
authors: RJ
authors: Langley
authors: Isabel
authors: I
authors: Piazzon
authors: Roy A
authors: RA
authors: Mariuzza
authors: Emilio L
authors: EL
authors: Malchiodi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004768:Enterotoxins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D015798:HLA-DR1 Antigen / Q000187:drug effects*; D000949:Histocompatibility Antigens Class II / Q000187:drug effects; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000819:agonists*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D018089:Superantigens / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D020349:Surface Plasmon Resonance; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology
chemical_list: D004768:Enterotoxins; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C419397:enterotoxin G, staphylococcal; C419398:enterotoxin I, staphylococcal