The potential of phage display virions expressing malignant tumor specific antigen MAGE-A1 epitope in murine model.
The tumor specific antigen epitope melanoma antigen A1(161--169) (MAGE-A1(161--169)) was displayed on the major protein (p VIII) of the filamentous bacteriophage (fd). The immune responses of the phage display particles expressing MAGE-A1(161--169) in mice were studied. Using phage display particles as vaccine was effective in affording protection from tumor growth, inducing growth control of established tumors and prolonging survival of tumor-bearing mice. The hybrid phage particles elicited MAGE-A1(161--169) specific CTL responses, NK activity and DTH. Our results showed that the phage display particles might be a new vaccine candidate for tumor-associated antigen epitope in cancer immunotherapy.
Fang
Jinbo
J
School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Nanguan District, Changchun 130024, PR China.
Wang
Guiyun
G
Yang
Qiong
Q
Song
Jinna
J
Wang
Ye
Y
Wang
Li
L
D000818:Animals; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007694:Killer Cells, Natural / Q000276:immunology; D058950:Melanoma-Specific Antigens; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000276:immunology*; D009368:Neoplasm Transplantation; D019151:Peptide Library; D014771:Virion / Q000276:immunology
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D000939:Epitopes; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D019151:Peptide Library