[The effects of TNF alpha and IFN gamma on the expression of pattern recognition receptors on the surface of mouse alveolar macrophages].
OBJECTIVE
To investigate the effects of tumor necrosis factor alpha (TNF alpha) and interferon gamma (IFN gamma) on the expression of pattern recognition receptors (PRRs) on the surface of mouse alveolar macrophages.


METHODS
Alveolar macrophages from mouse were cultured in DMEM supplemented with 10% (V/V) endotoxin-free calf serum. After the alveolar macrophages were stimulated with TNF alpha and IFN gamma (concentration, 20 ng/ml) for 3 h, 6 h and 12 h, the expression of PRRs, including cluster of differentiation 14 (CD14), scavenger receptor (SR), toll-like receptor 4 (TLR4), TLR2 and TLR9 mRNA and proteins were examined by RT-PCR and immunohistochemistry.


RESULTS
The expressions of CD14, TLR2 and TLR9 receptors, which were related with cellular activation, were up-regulated by the stimulation of TNF alpha and IFN gamma (P < 0.05), while SR, which was related with cellular defense action, was down-regulated (P < 0.05). Although the expression of TLR4 was up-regulated, there was no statistical significance (P > 0.05).


CONCLUSIONS
The cytokines such as TNF alpha and IFN gamma could also produce feedback regulation on the expression of PRRs at the levels of genes and proteins. Such regulation on the PRRs expression would be significant for further amplification of inflammation cascade and eventually leading to uncontrolled inflammation.
Huang
Hong
H
Research Institute for Traffic Medicine, Department 4, Institute of Field Surgery Research, The Third Affiliated Hospital, Third Military Medical University, Chongqing 400042, China. huanghongcq@hotmail.com
Jiang
Jian-xin
JX
Zhu
Pei-fang
PF
Wang
Zheng-guo
ZG
Zhang
Dao-jie
DJ
Yang
Cheng
C
D000818:Animals; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics; D016676:Macrophages, Alveolar / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D051192:Receptors, Pattern Recognition / Q000096:biosynthesis*; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D051192:Receptors, Pattern Recognition; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma