title: Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif.
abstract: Fragile X mental retardation protein (FMRP), the protein responsible for the fragile X syndrome, is an RNA-binding protein involved in localization and translation of neuronal mRNAs. One of the RNAs known to interact with FMRP is the dendritic non-translatable brain cytoplasmic RNA 1 BC1 RNA that works as an adaptor molecule linking FMRP and some of its regulated mRNAs. Here, we showed that the N terminus of FMRP binds strongly and specifically to BC1 and to its potential human analog BC200. This region does not contain a motif known to specifically recognize RNA and thus constitutes a new RNA-binding motif. We further demonstrated that FMRP recognition involves the 5' stem loop of BC1 and that this is the region that exhibits complementarity to FMRP target mRNAs, raising the possibility that FMRP plays a direct role in BC1/mRNA annealing.
authors: Dipartimento di Biologia, Università Tor Vergata, 00133 Rome, Italy.
authors: Francesca
authors: F
authors: Zalfa
authors: Salvatore
authors: S
authors: Adinolfi
authors: Ilaria
authors: I
authors: Napoli
authors: Eva
authors: E
authors: Kühn-Hölsken
authors: Henning
authors: H
authors: Urlaub
authors: Tilmann
authors: T
authors: Achsel
authors: Annalisa
authors: A
authors: Pastore
authors: Claudia
authors: C
authors: Bagni
mesh_terms: D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005600:Fragile X Syndrome / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020733:RNA, Small Cytoplasmic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: C117734:BC1 RNA; D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D020733:RNA, Small Cytoplasmic; D016601:RNA-Binding Proteins