[Comparison of two amine-modified chemical platforms for DNA microarray preparation].
OBJECTIVE
To study two amine modification procedures for DNA microarray preparation based on polymeric coatings.


METHODS
One of the proposed approaches utilized poly-amine coating of silanized slides activated by 1,4-phenylene diisothiocyanate, and the other employed acrylic acid-co-acrylamide copolymer and 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide as the coating agents and activator, respectively. The modified slides were used for preparing lambda phage DNA microarrays, whose properties were analyzed by hybridization.


RESULTS
Formation of dendrimeric structure and polymer was observed on the surface of the slides. The signal spots in uniform, steady and regular shape, in comparison with the commercial CMT-GAPS slides, indicated successful manufacture of the microarrays.


CONCLUSIONS
The two platforms are suitable for microarray preparation, and the method of acrylic acid-co-acrylamide copolymer modification is more preferred.
Wu
Qing-hua
QH
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. wuqh@fimmu.com
Ma
Wen-li
WL
Wang
Hong-min
HM
Mao
Xiang-ming
XM
Zhang
Bao
B
Li
Ling
L
Zheng
Wen-ling
WL
D000178:Acrylamides / Q000737:chemistry*; D000588:Amines / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011108:Polymers / Q000737:chemistry
D000178:Acrylamides; D000588:Amines; D011108:Polymers; C431184:poly(acrylamide-co-acrylic acid)