Protective effect of egg yolk phosvitin against ultraviolet- light-induced lipid peroxidation in the presence of iron ions.
It is known that nonheme iron accumulates and free radicals are generated in skin exposed to ultraviolet (UV) light. Iron ions have a role in skin photodamage by participating in the formation of reactive oxygen species. In this study, we evaluated the effect of egg yolk phosvitin on UV-light-induced oxidative stress. Mouse dorsal skin homogenate was exposed to UVA light in the presence or absence of ferric nitrilotriacetate (FeNTA). Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances (TBARS). The TBARS concentration increased with increasing FeNTA concentration and UV-light-exposure time. In the presence of FeNTA, phosvitin more effectively inhibited in vitro lipid peroxidation than did bovine serum albumin. According to results of electron spin resonance studies using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent, phosvitin suppressed the formation of hydroxyl radicals. These results suggest that UV-light-induced oxidative stress can be reduced by phosvitin.
Ishikawa
Shin-ichi
S
Faculty of Animal Science, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
Ohtsuki
Shizuna
S
Tomita
Kentarou
K
Arihara
Keizo
K
Itoh
Makoto
M
D000818:Animals; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D017665:Hydroxyl Radical; D007477:Ions; D007501:Iron / Q000378:metabolism*; D015227:Lipid Peroxidation; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress; D010774:Phosvitin / Q000378:metabolism / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D019097:Spin Trapping; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors; D014466:Ultraviolet Rays
D003497:Cyclic N-Oxides; D007477:Ions; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D010774:Phosvitin; D007501:Iron