title: The genome of the kinetoplastid parasite, Leishmania major.
abstract: Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.
authors: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK. alicat@sanger.ac.uk
authors: Alasdair C
authors: AC
authors: Ivens
authors: Christopher S
authors: CS
authors: Peacock
authors: Elizabeth A
authors: EA
authors: Worthey
authors: Lee
authors: L
authors: Murphy
authors: Gautam
authors: G
authors: Aggarwal
authors: Matthew
authors: M
authors: Berriman
authors: Ellen
authors: E
authors: Sisk
authors: Marie-Adele
authors: MA
authors: Rajandream
authors: Ellen
authors: E
authors: Adlem
authors: Rita
authors: R
authors: Aert
authors: Atashi
authors: A
authors: Anupama
authors: Zina
authors: Z
authors: Apostolou
authors: Philip
authors: P
authors: Attipoe
authors: Nathalie
authors: N
authors: Bason
authors: Christopher
authors: C
authors: Bauser
authors: Alfred
authors: A
authors: Beck
authors: Stephen M
authors: SM
authors: Beverley
authors: Gabriella
authors: G
authors: Bianchettin
authors: Katja
authors: K
authors: Borzym
authors: Gordana
authors: G
authors: Bothe
authors: Carlo V
authors: CV
authors: Bruschi
authors: Matt
authors: M
authors: Collins
authors: Eithon
authors: E
authors: Cadag
authors: Laura
authors: L
authors: Ciarloni
authors: Christine
authors: C
authors: Clayton
authors: Richard M R
authors: RM
authors: Coulson
authors: Ann
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authors: Cronin
authors: Angela K
authors: AK
authors: Cruz
authors: Robert M
authors: RM
authors: Davies
authors: Javier
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authors: De Gaudenzi
authors: Deborah E
authors: DE
authors: Dobson
authors: Andreas
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authors: Duesterhoeft
authors: Gholam
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authors: Fazelina
authors: Nigel
authors: N
authors: Fosker
authors: Alberto Carlos
authors: AC
authors: Frasch
authors: Audrey
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authors: Gabel
authors: Arlette
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authors: Goble
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authors: Goffeau
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authors: Harris
authors: Christiane
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authors: Hertz-Fowler
authors: Helmut
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authors: Hilbert
authors: David
authors: D
authors: Horn
authors: Yiting
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authors: Huang
authors: Sven
authors: S
authors: Klages
authors: Andrew
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authors: Knights
authors: Michael
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authors: Kube
authors: Natasha
authors: N
authors: Larke
authors: Lyudmila
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authors: Litvin
authors: Angela
authors: A
authors: Lord
authors: Tin
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authors: Louie
authors: Marco
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authors: Marra
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authors: Masuy
authors: Keith
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authors: Matthews
authors: Shulamit
authors: S
authors: Michaeli
authors: Jeremy C
authors: JC
authors: Mottram
authors: Silke
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authors: Müller-Auer
authors: Heather
authors: H
authors: Munden
authors: Siri
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authors: Nelson
authors: Halina
authors: H
authors: Norbertczak
authors: Karen
authors: K
authors: Oliver
authors: Susan
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authors: O'neil
authors: Martin
authors: M
authors: Pentony
authors: Thomas M
authors: TM
authors: Pohl
authors: Claire
authors: C
authors: Price
authors: Bénédicte
authors: B
authors: Purnelle
authors: Michael A
authors: MA
authors: Quail
authors: Ester
authors: E
authors: Rabbinowitsch
authors: Richard
authors: R
authors: Reinhardt
authors: Michael
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authors: Rieger
authors: Joel
authors: J
authors: Rinta
authors: Johan
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authors: Robben
authors: Laura
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authors: Robertson
authors: Jeronimo C
authors: JC
authors: Ruiz
authors: Simon
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authors: Rutter
authors: David
authors: D
authors: Saunders
authors: Melanie
authors: M
authors: Schäfer
authors: Jacquie
authors: J
authors: Schein
authors: David C
authors: DC
authors: Schwartz
authors: Kathy
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authors: Seeger
authors: Amber
authors: A
authors: Seyler
authors: Sarah
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authors: Sharp
authors: Heesun
authors: H
authors: Shin
authors: Dhileep
authors: D
authors: Sivam
authors: Rob
authors: R
authors: Squares
authors: Steve
authors: S
authors: Squares
authors: Valentina
authors: V
authors: Tosato
authors: Christy
authors: C
authors: Vogt
authors: Guido
authors: G
authors: Volckaert
authors: Rolf
authors: R
authors: Wambutt
authors: Tim
authors: T
authors: Warren
authors: Holger
authors: H
authors: Wedler
authors: John
authors: J
authors: Woodward
authors: Shiguo
authors: S
authors: Zhou
authors: Wolfgang
authors: W
authors: Zimmermann
authors: Deborah F
authors: DF
authors: Smith
authors: Jenefer M
authors: JM
authors: Blackwell
authors: Kenneth D
authors: KD
authors: Stuart
authors: Bart
authors: B
authors: Barrell
authors: Peter J
authors: PJ
authors: Myler
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D020459:Genes, rRNA; D018503:Genome, Protozoan; D006001:Glycoconjugates / Q000096:biosynthesis / Q000378:metabolism; D018320:Leishmania major / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D006001:Glycoconjugates; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan