Characterization of estrogen-induced F-box protein FBXO45.
Members of the F-box protein family are characterized by an approximately 40-amino-acid F-box domain. SCF complexes, formed by Skp1, Cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with Skp1 through the F-box, and with ubiquitination targets. Therefore, F-box proteins are implicated in cell cycle regulation and signal transduction. We characterized the FBXO45 gene, a recently identified F-box protein, as an estrogen-induced gene. By using bioinformatics, human and mouse FBXO45 (286 aa) showed 100% total-amino-acid identity. Human and mouse FBXO45 genes consisting of 3 exons, and the vicinity of the transcriptional start site had several estrogen receptor binding consensus sequences. Finally, human FBXO45 mRNA was rapidly elevated by 17beta-estradiol in MCF-7 cells.
Yoshida
Kenichi
K
Department of Life Sciences, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan. yoshida@isc.meiji.ac.jp
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D004958:Estradiol / Q000494:pharmacology*; D005091:Exons; D044783:F-Box Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D044783:F-Box Proteins; C503710:FBXO45 protein, human; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D004958:Estradiol