The Trypanosoma cruzi proteome.
To complement the sequencing of the three kinetoplastid genomes reported in this issue, we have undertaken a whole-organism, proteomic analysis of the four life-cycle stages of Trypanosoma cruzi. Peptides mapping to 2784 proteins in 1168 protein groups from the annotated T. cruzi genome were identified across the four life-cycle stages. Protein products were identified from >1000 genes annotated as "hypothetical" in the sequenced genome, including members of a newly defined gene family annotated as mucin-associated surface proteins. The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes.
Atwood
J A
JA
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
Weatherly
D B
DB
Minning
T A
TA
Bundy
B
B
Cavola
C
C
Opperdoes
F R
FR
Orlando
R
R
Tarleton
R L
RL
D000222:Adaptation, Physiological; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D002853:Chromatography, Liquid; D019295:Computational Biology; D030541:Databases, Genetic; D004734:Energy Metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics; D006639:Histidine / Q000378:metabolism; D008018:Life Cycle Stages; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D009077:Mucins / Q000032:analysis; D005810:Multigene Family; D009439:Neuraminidase / Q000032:analysis / Q000235:genetics; D010455:Peptides / Q000032:analysis; D021381:Protein Transport; D020543:Proteome; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D000953:Antigens, Protozoan; D004798:Enzymes; D006023:Glycoproteins; D008565:Membrane Proteins; D009077:Mucins; D010455:Peptides; D020543:Proteome; D015800:Protozoan Proteins; D006639:Histidine; C069715:trans-sialidase; D009439:Neuraminidase