Nucleic acid complexing glycosyl nucleoside-based amphiphile.
A neutral amphiphile derived from uridine featuring two oleyl chains and one glucose for DNA binding was prepared using a convenient four-step synthetic route. The nucleic acid binding capabilities of this amphiphile were investigated by UV-vis, quasi-elastic light scattering (QELS), transmission electronic microscopy (TEM), gel electrophoresis, 31P NMR, IR, and circular dichroism (CD). Amphiphile-nucleic acid complex formation is a consequence of the amphiphilic character of the molecule, phosphate-sugar, and nucleobase-nucleobase interactions. This work presents for the first time a glyco-nucleo-amphiphile capable of binding efficiently the nucleic acid double helix structure.
Arigon
Jerome
J
Faculté des sciences d'Avignon, 33 rue Louis Pasteur, F-84000 Avignon, France.
Prata
Carla A H
CA
Grinstaff
Mark W
MW
Barthélémy
Philippe
P
D000818:Animals; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D005947:Glucose / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
D009696:Nucleic Acids; D009705:Nucleosides; D005947:Glucose