Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing.
To determine the genotypes of Giardia intestinalis from domestic and wild animals in Japan, Giardia isolates obtained from feces of 24 dogs kept in households and breeding kennels, three companion cats, five dairy calves and three wild monkeys, Macaca fuscata, were genotyped using the 177 bp sequence of the glutamete dehydrogenase gene (gdh). The genotypes were assemblages A, C, D or A/D for dog isolates, Assemblage F for cat isolates, assemblages A or E for calf isolates and assemblage B for monkey isolates. This is the first report on the genotypes of Giardia isolates from cats, calves and wild monkeys in Japan.
Itagaki
Tadashi
T
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-0105, Japan. itagaki@iwate-u.ac.jp
Kinoshita
Shizuka
S
Aoki
Mikiko
M
Itoh
Naoyuki
N
Saeki
Hideharu
H
Sato
Naoto
N
Uetsuki
Junya
J
Izumiyama
Shinji
S
Yagita
Kenji
K
Endo
Takuro
T
D000818:Animals; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005873:Giardiasis / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D000882:Haplorhini; D008992:Monkey Diseases / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D005969:Glutamate Dehydrogenase