X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi.
Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA3 staining reveals that these silver positive telomeres are the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA.
Criniti
A
A
Dipartimento di Scienze Agrarie, Università di Modena e Reggio Emilia, Reggio Emilia, Italia.
Simonazzi
G
G
Cassanelli
S
S
Ferrari
M
M
Bizzaro
D
D
Manicardi
G C
GC
D000818:Animals; D001042:Aphids / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006570:Heterochromatin / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence; D014960:X Chromosome / Q000737:chemistry
D004275:DNA, Ribosomal; D006570:Heterochromatin