Differentially expressed genes in endothelial differentiation.
By screening differentially expressed genes in mouse embryonic stem (ES) cells by subtractive hybridization, we identified three conserved but uncharacterized genes encoding bromodomain containing 3 (BRD3), protein lysine methyltransferase (PLM), and kelch domain containing 2 (KLHDC2), which were downregulated during endothelial differentiation. An RNA blot study showed that these genes were markedly expressed in undifferentiated ES cells, whereas the expression was reduced upon endothelial differentiation; a study of mouse endothelium showed a significant reduction in the expression of BRD3. A study of human BRD3, located on chromosome 9 at q34, a region susceptible to genomic rearrangement, showed an altered expression in 4 of 12 patients with bladder cancer, compared with adjacent noncancerous tissues. Taken together with the result of siRNA inhibition showing the positive regulation of cell proliferation by BRD3, it is suggested that this molecule plays a role in allowing cells to enter the proliferative phase of the angiogenic process.
Ishii
Hideshi
H
Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan. hishiiAms.jichi.ac.jp
Mimori
Koshi
K
Mori
Masaki
M
Vecchione
Andrea
A
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002899:Chromosomes, Human, Pair 9; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000166:cytology*; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000196:embryology; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010179:Pancreas / Q000098:blood supply / Q000166:cytology; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000037:antagonists & inhibitors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology; D000092468:Bromodomain Containing Proteins
D011495:Histone-Lysine N-Methyltransferase; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C518476:Brd3 protein, mouse; D000092468:Bromodomain Containing Proteins