[Raman spectral study of nitrosyhemoglobin and several other hemoglobins].
The Raman spectra of nitrosyhemoglobin (NOHb), methemoglobin (MetHb), oxygenated hemoglobin (oxyHb), and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggestingthat Raman spectra provide anew simple way to measure and distinguish NOHb and MetHb.
Cen
Yan
Y
Department of Physics, Fudan University, Shanghai 200433, China.
Zhang
Ren
R
Yao
Wen-hua
WH
Ma
Jiong
J
Chen
Ji-yao
JY
D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008706:Methemoglobin / Q000737:chemistry*; D010108:Oxyhemoglobins / Q000737:chemistry*; D015203:Reproducibility of Results; D013059:Spectrum Analysis, Raman / Q000379:methods*
D006454:Hemoglobins; D010108:Oxyhemoglobins; C008657:nitrosyl hemoglobin; C100003:deoxyhemoglobin; D008706:Methemoglobin