title: Susceptibility and immune responses following experimental infection of MHC compatible Atlantic salmon (Salmo salar L.) with different infectious salmon anaemia virus isolates.
abstract: Infectious salmon anaemia virus (ISAV) is an aquatic orthomyxovirus causing a multisystemic disease in farmed Atlantic salmon (Salmo salar) where disease development, clinical signs, and histopathology vary to a large extent. Here, an experimental trial was designed to determine the effect of variation in viral genes on virus-host interactions, as measured by disease susceptibility and immune responses. The fish were infected using cohabitant transmission, representing a natural route of infection. Variation caused by host factors was minimized using MHC compatible A. salmon half-siblings as experimental fish. Virus isolates were selected according to HE genotype, as European ISAV isolates can be genotyped according to deletion patterns in their hemagglutinin-esterase (HE) surface glycoprotein, and the course of disease they typically induce, classified as acute versus protracted. The different ISAV isolates induced large variations in death prevalence, ranging from 0-47% in the test-group and 3-75% in the cohabitant fish. The use of MHC compatible experimental fish made it possible to determine the relative contribution of humoral versus cellular response in protection against ISA. Ability to induce a strong proliferative response correlated with survival and virus clearance, while induction of a humoral response was less protective.
authors: Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway. siri.mjaaland@veths.no
authors: S
authors: S
authors: Mjaaland
authors: T
authors: T
authors: Markussen
authors: H
authors: H
authors: Sindre
authors: S
authors: S
authors: Kjøglum
authors: B H
authors: BH
authors: Dannevig
authors: S
authors: S
authors: Larsen
authors: U
authors: U
authors: Grimholt
mesh_terms: D000740:Anemia / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D000818:Animals; D005393:Fish Diseases / Q000453:epidemiology / Q000401:mortality / Q000821:virology*; D008285:Major Histocompatibility Complex; D009975:Orthomyxoviridae; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000401:mortality / Q000821:virology*; D020317:Salmo salar / Q000276:immunology / Q000821:virology*