title: Morphological and molecular characterization of a Cypovirus (Reoviridae) from the mosquito Uranotaenia sapphirina (Diptera: Culicidae).
abstract: A novel cypovirus has been isolated from the mosquito Uranotaenia sapphirina (UsCPV) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. The production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. The virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a central core that is surrounded by a single capsid layer. They are usually packaged individually within cubic inclusion bodies (polyhedra, approximately 100 nm across), although two to eight virus particles were sometimes occluded together. The virus was experimentally transmitted per os to several mosquito species. The transmission rate was enhanced by the presence of magnesium ions but was inhibited by calcium ions. Most of the infected larvae survived to adulthood, and the adults retained the infection. Electrophoretic analysis of the UsCPV genome segments (using 1% agarose gels) generated a migration pattern (electropherotype) that is different from those of the 16 Cypovirus species already recognized. UsCPV genome segment 10 (Seg-10) showed no significant nucleotide sequence similarity to the corresponding segment of the other cypoviruses that have previously been analyzed, and it has different "conserved" termini. A BLAST search of the UsCPV deduced amino acid sequence also showed little similarity to Antheraea mylitta CPV-4 (67 of 290 [23%]) or Choristoneura fumiferana CPV-16 (33 of 111 [29%]). We conclude that UsCPV should be recognized as a member of a new Cypovirus species (Cypovirus 17, strain UsCPV-17).
authors: Center for Medical, Agricultural and Veterinary Entomology, USDA/ARS, Gainesville, FL 32604, USA.
authors: Alexandra
authors: A
authors: Shapiro
authors: Terry
authors: T
authors: Green
authors: Shujing
authors: S
authors: Rao
authors: Susan
authors: S
authors: White
authors: Gerry
authors: G
authors: Carner
authors: Peter P C
authors: PP
authors: Mertens
authors: James J
authors: JJ
authors: Becnel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009033:Culicidae / Q000648:ultrastructure / Q000821:virology*; D003593:Cytoplasm / Q000821:virology; D004847:Epithelial Cells / Q000821:virology; D007422:Intestines / Q000821:virology; D007814:Larva / Q000382:microbiology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure*; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D013270:Stomach / Q000821:virology
chemical_list: D012367:RNA, Viral