[Primary research of reconstruction of complete biological tissue engineered blood vessel].
To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells (VSMCs) were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.
Zhang
Yan
Y
Department of Human Anatomy, Second Military Medical University, Shanghai 200433, China.
Liu
Taihua
T
Jiang
Zonglai
Z
Zhang
Xiuhua
X
Liu
Bo
B
Li
Yuquan
Y
D000818:Animals; D001158:Arteries; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D018920:Coculture Techniques; D004285:Dogs; D046529:Microscopy, Electron, Transmission; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds