Cytotoxic effect and electrophysiological activity of S-irniine, a synthesised isomer of the natural R-irniine, on human MRC-5 fibroblasts.
Numerous original alkaloids are present in tubers of Arisarum vulgare Targ. a species belonging to the Araceae family known in Morocco for its toxicity. Some previous works deal with the activity of these natural compounds as R-irniine. As the enantioselective total synthesis of irniine has been realised, the R- and S-forms were obtained and this last one could be tested. Thus, a study of the cytotoxicity and the electrophysiological activity of S-irniine was carried out on human MRC-5 fibroblasts. A cytotoxic effect of S-irniine at 40 microg/mL was detected on MRC-5 fibroblasts. An electrophysiological study was also carried out on the MRC-5 cells by using the patch-clamp technique and no effect of this compound at this concentration on the outward potassium current of MRC-5 fibroblasts was observed. Thus, this study showed, as it was for R-irniine, that the cytotoxicity of S-irniine was not explained by an effect on the potassium currents.
Lamkadem
Mohammed
M
Laboratory of Phytochemistry and Pharmacognosy, University Mohamed Ier, Route Sidi Maafa, B.P. 524, 60000, Oujda, Morocco.
Schwob
Isabelle
I
Mimouni
Jamal
J
Aziz
Mohammed
M
Bouali
Abderrahime
A
Rabier
Jacques
J
Fontonge
Robert
R
Melhaoui
Ahmed
A
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D029064:Araceae / Q000737:chemistry*; D018929:Cell Culture Techniques; D004594:Electrophysiology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007536:Isomerism; D018408:Patch-Clamp Techniques; D011759:Pyrrolidines / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*
D000470:Alkaloids; D011759:Pyrrolidines; C411262:irniine