Comparison of bioMérieux API 20NE and Remel RapID NF Plus, identification systems of type strains of Ralstonia pickettii.
A : Comparison of two commercial miniaturized rapid systems for the identification of Ralstonia pickettii strains.


RESULTS
Varying identification results were encountered using the bioMérieux API NE system and the Remel IDS RapID NF Plus commercial systems for R. pickettii. To compare these two systems, eight strains of R. pickettii were purchased from different commercial culture collections. Additionally, 32 industrial and eight clinical isolates, initially identified using the Vitek Junior (bioMérieux) were tested. Total number of isolates tested was 48. The API 20NE identified 29 isolates, as R. pickettii but was unsuccessful with 19 isolates. The Remel IDS RapID NF Plus identified 46 isolates as R. pickettii. One clinical and one industrial isolates was identified as non-R. pickettii with both systems.


CONCLUSIONS
The above results indicate that the use of API 20NE system for examining the identification of R. pickettii strains is inconsistent.


CONCLUSIONS
This study demonstrated that the RapID NF Plus is more accurate as an inexpensive identification system for the identification of R. pickettii, a potential emerging organism of medically and industrial importance.
Adley
C C
CC
Microbiology Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland. catherine.adley@ul.ie
Saieb
F M
FM
D015373:Bacterial Typing Techniques; D006801:Humans; D049920:Ralstonia pickettii / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism