title: CD8+ T-cell tolerance induced by delivery of antigen to the anterior chamber is not the result of de facto intravenous or mucosal administration of antigen.
abstract: OBJECTIVE
  We tested whether antigen administration via the anterior chamber (a.c.) was equivalent to intravenous (i.v.) or mucosal administration antigen.
  
  
  METHODS
  Ovalbumin (OVA)-specific CD8(+) T cells (OT-I) were enumerated in lymphoid tissues of C57Bl/6 (B6) mice via adoptive transfer after the same amount of antigen was administered via a.c., i.v., or mucosal routes. Lytic activity was measured in B6 and gammadeltaT cell-deficient B6 mice given OVA via a.c., i.v, or mucosal routes after injection with OVA in adjuvant.
  
  
  RESULTS
  OVA a.c. induced a pattern of T-cell proliferation distinct from i.v. or mucosal administration. A.c. and i.v., but not mucosal, OVA induced cytolytic T lymphocyte (CTL) tolerance. The inhibition of CTL responses was significantly greater in mice given OVA a.c. rather than i.v. gammadeltaT cells contributed to a.c.-, but not i.v.-, induced CTL tolerance.
  
  
  CONCLUSIONS
  A.c. administration of antigen not de-facto i.v. or mucosal administration of antigen.
authors: Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA, USA.
authors: Kyle C
authors: KC
authors: McKenna
authors: Kimberly M
authors: KM
authors: Anderson
authors: Judith A
authors: JA
authors: Kapp
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000867:Anterior Chamber / Q000166:cytology / Q000276:immunology*; D016827:CD8 Antigens / Q000008:administration & dosage*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D007108:Immune Tolerance / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007267:Injections / Q000379:methods; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D016827:CD8 Antigens; D010047:Ovalbumin