Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor.
This paper describes a microfluidic channel that allows for diffusion-based analysis of adsorbing species without passivation of the channel surfaces. The sheath flow configuration was used to measure the diffusion coefficient of fluorescently labeled species from their spatial distribution within the microchannel by analyzing the derivative of the intensity profile at the interface between two distinct core fluids. Measurements for both a small molecule (rhodamine B) and an intermediate-sized protein (wheat germ agglutinin) were made, demonstrating the utility of the sheath flow T-sensor.
Munson
Matthew S
MS
University of Washington, Department of Bioengineering, Box 352255, Seattle, WA, USA. msmunson@u.washington.edu
Hawkins
Kenneth R
KR
Hasenbank
Melissa S
MS
Yager
Paul
P
D004058:Diffusion; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D012235:Rhodamines / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D012235:Rhodamines; D014909:Wheat Germ Agglutinins; C029773:rhodamine B