Crystal structure of YHI9, the yeast member of the phenazine biosynthesis PhzF enzyme superfamily.
In the Pseudomonas bacterial genomes, the PhzF proteins are involved in the production of phenazine derivative antibiotic and antifungal compounds. The PhzF superfamily however also encompasses proteins in all genomes from bacteria to eukaryotes, for which no function has been assigned. We have determined the three dimensional crystal structure at 2.05 A resolution of YHI9, the yeast member of the PhzF family. YHI9 has a fold similar to bacterial diaminopimelate epimerase, revealing a bimodular structure with an internal symmetry. Residue conservation identifies a putative active site at the interface between the two domains. Evolution of this protein by gene duplication, gene fusion and domain swapping from an ancestral gene containing the "hot dog" fold, identifies the protein as a "kinked double hot dog" fold.
Liger
Dominique
D
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire (CNRS-UMR 8619), Université Paris-Sud, Orsay, France.
Quevillon-Cheruel
Sophie
S
Sorel
Isabelle
I
Bremang
Michael
M
Blondeau
Karine
K
Aboulfath
Ilham
I
Janin
Joël
J
van Tilbeurgh
Herman
H
Leulliot
Nicolas
N
D000591:Amino Acid Isomerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C508553:YHI9 protein, S cerevisiae; D000591:Amino Acid Isomerases; C042032:diaminopimelate epimerase