[Pharmacokinetics of ginsenosides Rg1 and Re in Shenmai injection].
OBJECTIVE
To study the pharmacokinetics of ginsenosides Rg1 and Re after iv infusion of Shenmai injection in human.


METHODS
Ginsenosides Rg1 and Re in plasma were determined by LC/MS/MS and the pharmacokinetic parameters were calculated.


RESULTS
The linear regressive curves were obtained in the range of 1.023-1023 microg x L(-1) for Rg1 and 1.05-1050 microg x L(-1) for Re. Recoveries using the method of Rg1 and Re were 99%-105% and 99%-104%, respectively. The within-day and between-day RSDs were less than 15%. After iv infusion of Shenmai injection to volunteers, the concentration-time curves of Rg1 and Re fitted to the two-compartment model, T1/2alpha were 0.28 h and 0.10 h, T1/2beta were 2.1 h and 1.2 h, respectively.


CONCLUSIONS
The method is specific, simple, sensitive and suitable for the measurement of plasma Rg1 and Re concentrations. The distribution and elimination of Rg1 and Re were rapid after iv infusion of Shenmai injection in volunteers, the pharmacokinetic characteristics were fitted with the two-compartment model.
Liu
Yi-ming
YM
Department of Clinical Pharmacology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China.
Yang
Liu
L
Zeng
Xing
X
Deng
Yuan-hui
YH
Feng
Yi
Y
Liang
Wei-xiong
WX
D019540:Area Under Curve; D002853:Chromatography, Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005260:Female; D036145:Ginsenosides / Q000097:blood / Q000493:pharmacokinetics*; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D031441:Ophiopogon / Q000737:chemistry; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C049864:ginsenoside Re; C035054:ginsenoside Rg1