Improving alpha-tocopherol production in plant cell cultures.
Suspension cell cultures of Helianthus annuus L. were previously established for the production of the most active component of vitamin E, alpha-tocopherol, by optimizing medium composition and culture conditions. In the present work, the possibility of enhancing alpha-tocopherol production by the addition of jasmonic acid to the culture medium was investigated both in sunflower and Arabidopsis cell cultures. A considerable increase (49% and 66%, respectively) of alpha-tocopherol production was obtained in both, after a 72-h treatment with 5 microM jasmonic acid. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.
Gala
Rosa
R
Istituto di Scienze delle Produzioni Alimentari--Sezione di Lecce, CNR, Via Prov. le Lecce-Monteroni, 73100 Lecce, Italy.
Mita
Giovanni
G
Caretto
Sofia
S
D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006368:Helianthus / Q000187:drug effects / Q000378:metabolism*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000378:metabolism*
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D024502:alpha-Tocopherol