title: [Detection rate of human papillomavirus-16 in esophageal squamous cell carcinoma from different Chinese populations].
abstract: OBJECTIVE
  Anyang in Henan Province of China is a hyperendemic area of esophageal cancer. The infection of human papillomavirus (HPV) is thought as an important pathogenesis of esophageal cancer in Anyang. This study was to detect infection rate and level of HPV-16 in esophageal squamous cell carcinoma (ESCC) patients from 3 different Chinese areas, and investigate its relationship with the pathogenesis of ESCC.
  
  
  METHODS
  Infection status of HPV-16 in 119 ESCC specimens (43 collected from Anyang, 43 from Beijing, and the rest 33 from Mongolia nationality of Inner Mongolia) was detected by in situ hybridization (ISH) technique with digoxin-labeled HPV-16 E6 probe.
  
  
  RESULTS
  HPV16 infection rates were 81.4%, 69.8%, and 63.6% in the specimens from Anyang, Beijing, and Inner Mongolia, respectively. Infection level of HPV-16 was significantly higher in Anyang group than in Beijing group (H=3.91, P<0.05) and Inner Mongolia group(H=4.22,P<0.05). There was no significant difference between the latter 2 groups. Furthermore, the proportion of strong expression of HPV16 (++ and +++) was significantly higher in Anyang group than in the other 2 groups(H=3.95, P<0.05).
  
  
  CONCLUSIONS
  HPV-16 infection rate is high in the esophageal specimens from the 3 different areas. Infection status of HPV16 is serious in Anyang.
authors: Department of Genetics, Beijing Institute of Cancer Research/Beijing Cancer Hospital/ School of Clinical Oncology, Peking University, Beijing, 100034, PR China.
authors: Lin-Zhong
authors: LZ
authors: Zhu
authors: Xiu-Lan
authors: XL
authors: Su
authors: Ke-Neng
authors: KN
authors: Chen
authors: Ren-Jie
authors: RJ
authors: Yang
authors: Hai-Ping
authors: HP
authors: Xing
authors: Jian-Guo
authors: JG
authors: Cui
authors: Yang
authors: Y
authors: Ke
mesh_terms: D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D002681:China / Q000453:epidemiology; D015344:DNA Probes, HPV / Q000302:isolation & purification*; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000821:virology*; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology
chemical_list: D015344:DNA Probes, HPV