The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori.
As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.
Hwang
Jae-Sam
JS
Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea. hwangjs@rda.go.kr
Go
Hyun-Jeong
HJ
Goo
Tae-Won
TW
Yun
Eun-Young
EY
Choi
Kwang-Ho
KH
Seong
Su-Il
SI
Lee
Sang-Mong
SM
Lee
Bong-Hee
BH
Kim
Iksoo
I
Chun
Taehoon
T
Kang
Seok-Woo
SW
D000818:Animals; D015152:Blotting, Northern; D035163:Bombax / Q000196:embryology* / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012333:RNA, Messenger; D003576:Electron Transport Complex IV