Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol gel.
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is via room-temperature Schiff chemistry in which an alkoxysilane reagent (linker) with an aldehyde functional group links to an inactive amine on trypsin to form an imine bond. The proteolytic activity of the immobilized trypsin was measured by monitoring the formation of N alpha-benzoyl-L-arginine (BA), the digestion product of BAEE. The BA is separated from BAEE by capillary electrophoresis and detected downstream (18.5 cm from the microreactor) by absorption (254 nm). Using the Bradford assay, we determined that 97 ng of trypsin is bound to the 1-cm microreactor located at the entrance of capillary column. The bioactivity of the trypsin-PSG-PEG microreactor at 20 degrees C for the digestion of BAEE was found to be 2270 units/mg of immobilized trypsin. The bioactivity of trypsin bound to the capillary wall in the open segment upstream from the monolith was 332 units/mg of immobilized trypsin under the same conditions. In contrast, the activity of free trypsin could not be observed for the digestion of BAEE at 20 degrees C after 16 h of incubation time.
Dulay
Maria T
MT
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Baca
Quentin J
QJ
Zare
Richard N
RN
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005782:Gels / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry / Q000378:metabolism; D008027:Light; D009496:Neurotensin / Q000378:metabolism; D011092:Polyethylene Glycols; D021122:Protein Subunits; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005782:Gels; D007328:Insulin; D021122:Protein Subunits; D009496:Neurotensin; D011092:Polyethylene Glycols; D001120:Arginine; C009727:benzoylarginine ethyl ester; D014357:Trypsin