Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells.
OBJECTIVE
To induce apoptosis of endometriotic cells of patients with endometriosis.


METHODS
To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis.


METHODS
University hospital infertility center.


METHODS
Twelve women who visited the center for infertility therapy.


METHODS
Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment.


METHODS
Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining.


RESULTS
The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 microg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 microg of polyglycolic acid/chitosan glue A.


CONCLUSIONS
This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy.
Wang
Yu-Chi
YC
Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Fu
Ru-Huei
RH
Hsieh
Hsyue-Jen
HJ
Chao
Hsiang-Tai
HT
Kao
Shu-Huei
SH
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000473:pathology; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D011100:Polyglycolic Acid / Q000494:pharmacology*
D011100:Polyglycolic Acid; D048271:Chitosan