title: Acceleration of wound healing in healing-impaired db/db mice with a photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2.
abstract: Application of ultraviolet light irradiation to a photocrosslinkable chitosan (Az-CH-LA) aqueous solution including fibroblast growth factor-2 (FGF-2) results within 30 seconds in an insoluble, flexible hydrogel. The FGF-2 molecules retained in the chitosan hydrogel remain biologically active and are released from the chitosan hydrogel upon in vivo biodegradation of the hydrogel. To evaluate the accelerating effect on wound healing of this hydrogel, full-thickness skin incisions were made in the backs of healing-impaired diabetic (db/db) mice and their normal (db/+) littermates. The mice were later killed, and histological sections of the wound were prepared. The degree of wound healing was evaluated using several histological parameters such as the rate of contraction, epithelialization, and tissue filling. Application of the chitosan hydrogel significantly advanced the rate of contraction on Days 0 to 2 in db/db and db/+ mice. Although the addition of FGF-2 into the chitosan hydrogel in db/+ mice had little effect, application of the chitosan hydrogel-containing FGF-2 further accelerated the adjusted tissue filling rate (Days 2 to 4 and Days 4 to 8) in db/db mice. Furthermore, the chitosan hydrogel-containing FGF-2 markedly increased the number of CD-34-positive vessels in the wound areas of db/db mice on Day 4. Thus, the application of chitosan hydrogel-containing FGF-2 onto a healing-impaired wound induces significant wound contraction and accelerates wound closure and healing.
authors: Department of Surgery II, Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors: Kiyohaya
authors: K
authors: Obara
authors: Masayuki
authors: M
authors: Ishihara
authors: Masanori
authors: M
authors: Fujita
authors: Yasuhiro
authors: Y
authors: Kanatani
authors: Hidemi
authors: H
authors: Hattori
authors: Takemi
authors: T
authors: Matsui
authors: Bonpei
authors: B
authors: Takase
authors: Yuichi
authors: Y
authors: Ozeki
authors: Shingo
authors: S
authors: Nakamura
authors: Takamitsu
authors: T
authors: Ishizuka
authors: Susumu
authors: S
authors: Tominaga
authors: Sadayuki
authors: S
authors: Hiroi
authors: Toshiaki
authors: T
authors: Kawai
authors: Tadaaki
authors: T
authors: Maehara
mesh_terms: D000287:Administration, Topical; D000818:Animals; D048271:Chitosan / Q000008:administration & dosage*; D048909:Diabetes Complications / Q000150:complications; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D051379:Mice; D023421:Models, Animal; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000150:complications / Q000628:therapy*
chemical_list: D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan