title: Physico-chemical and biological evaluation of excimer laser irradiated polyethylene terephthalate (pet) surfaces.
abstract: The aim of this work was to investigate the consequences of excimer laser irradiation on the physico-chemical and biological properties of polyethylene terephthalate (PET) films, currently used for medical devices. Three PET films from different origins were studied in the present work, chosen with respect to their chemical and physical properties, which are of high importance for ulterior medical application as vascular prostheses. Multiple assays were carried out to characterize the physical and chemical effects of the laser irradiation: surface morphology tests (light microscopy, Dektak profilometer and confocal laser scanning microscopy) showed the strong transformation of the surface with the laser treatment. Contact angle measurements revealed a significant increase of the surface energy for each PET depending on the applied fluency. Finally XPS characterization of the surface demonstrated the appearance of new chemical species favorable for cell attachment. This aspect had to be strongly considered regarding to the multiple biological effects of laser irradiated surfaces on living cells. Different cell culture experiments were carried out with L132 human epithelial cells after 6-days culture: proliferation and vitality rate, cell adhesion and cell morphology. Results clearly revealed that laser treatment improved cell proliferation (up to 140% with respect to controls), vitality (10% higher than controls), morphology and adhesion kinetics (more than 16% of control). A significant correlation (R2=0.906) was also established on one PET between the fluencies of laser treatment and the cellular response. These results emphasized high importance of the choice of the PET material for a medical application: only one of the three considered PET films showed really improved cellular response.
authors: Laboratoire de Recherche sur les Biomatériaux, Département de Biophysique UPRES EA 1049, Faculté de Médecine, Université Lille-2, F-59045 Lille Cedex, France.
authors: G
authors: G
authors: Mayer
authors: N
authors: N
authors: Blanchemain
authors: C
authors: C
authors: Dupas-Bruzek
authors: V
authors: V
authors: Miri
authors: M
authors: M
authors: Traisnel
authors: L
authors: L
authors: Gengembre
authors: D
authors: D
authors: Derozier
authors: H F
authors: HF
authors: Hildebrand
mesh_terms: D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007834:Lasers; D008422:Materials Testing; D011093:Polyethylene Terephthalates / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates