Addressable electric fields for size-fractioned sample extraction in microfluidic devices.
Fraction collection following electrophoresis is of major importance for a variety of biological analyses. These assays typically need to identify specific fractions in the separated sample for further processing and require extraction of one or a group of fragments. In this paper, we have developed and characterized a technique to generate addressable electric fields for improved extraction during electrophoresis in microfluidic devices. The addressable electric field is achieved by applying a low bias voltage (1-2 V) to microelectrode pairs within the electrophoresis microchannel. Theoretical analysis shows the purity of the extracted sample can be improved as much as 30% over extraction without the shaped electric fields, and nearly 100% predicted yield can be achieved. We also describe the theoretical design of shaped electric fields by characterizing the optimal electrode geometry, field strength, channel configuration, and electrophoretic migration behavior needed for efficient band extraction.
Lin
Rongsheng
R
Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2136, USA.
Burke
David T
DT
Burns
Mark A
MA
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*
D004247:DNA