Identification of a feline coronavirus type I strain from a cat with feline infectious peritonitis by RT-pCR and phylogenetic analysis.
A case of feline infectious peritonitis (FIP) in an 11-month-old European shorthair cat is reported. The infected cat displayed loss of weight, respiratory distress, ascitis, anemia and died within 15 days after the first appearance of clinical signs. Lesions typical of a mixed form (effusive and non-effusive) of FIP were observed and by RT-PCR a feline coronavirus (FCoV) type I strain was detected in several tissues. The RT-PCR results were confirmed by sequence analysis of the amplified products. Phylogeny carried out on fragments of the M and S genes showed that the FCoV strain segregates with typical type I FCoVs.
Campolo
Marco
M
Department of Animal Health and Well-being, Faculty of Veterinary Medicine, Valenzano (BA), Italy. n.decaro@veterinaria.uniba.it
Desario
Costantina
C
Ricci
Dominga
D
Elia
Gabriella
G
Decaro
Nicola
N
Martella
Vito
V
Tempesta
Maria
M
Di Trani
Livia
L
Cavaliere
Nicola
N
Buonavoglia
Canio
C
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D016765:Coronavirus, Feline / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D017809:Fatal Outcome; D016766:Feline Infectious Peritonitis / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D004279:DNA, Viral