The transcription factor PLA-1/SKN-1A is expressed in human placenta and regulates the placental lactogen-3 gene expression.
Here we report the selective expression of two POU transcription factor genes, PLA-1 and OCT-1, in human placenta and choriocarcinoma cell lines JAR, JEG-3 and BeWo. Pla-1 protein binds to a POU-consensus DNA sequence in the human placental lactogen-3 (PL-3) promoter and it is capable of trans-activating its transcription up to 18-fold. Other tissue-specific or ubiquitous POU transcription factors such as Pit-1/GHF-1 or Oct-1 showed none or low levels of trans-activation of the PL-3 promoter. In addition, we identified an unique and highly charged region in the N-terminal portion of Pla-1 protein required for full trans-activation of the PL-3 promoter.
Jiménez-Mateo
O
O
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain.
Rodríguez-Torres
A
A
Avila
S
S
Castrillo
J-L
JL
D001665:Binding Sites; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D050809:POU Domain Factors / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators
D050809:POU Domain Factors; D015534:Trans-Activators; C511759:placental lactogen-3, human; C085699:placental lactogen I, rat; D010928:Placental Lactogen