Differential expression of the chsE gene encoding a chitin synthase of Aspergillus nidulans in response to developmental status and growth conditions.
Expression of chsE encoding one of the five chitin synthases of Aspergillus nidulans was analyzed. Expression of chsE was moderate in conidiophores, but somewhat weaker in vegetative mycelia. During sexual development, chsE was expressed strongly in young cleistothecia and hülle cells, but little in mature sexual structures. Deletion of chsE caused a significant decrease in the chitin content of the cell wall during early sexual development. Expression of chsE was increased by substituting glucose with lactose or by addition of 0.6M KCl or NaCl, but affected little by substituting glucose with sodium acetate. Consequently, chsE was shown to have a mode of expression distinct from those of the other chitin synthase genes, chsA, chsB and chsC.
Lee
Jeong Im
JI
Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Yu
Yeong Man
YM
Rho
Yoo Mi
YM
Park
Bum Chan
BC
Choi
Jeong Hwan
JH
Park
Hee-Moon
HM
Maeng
Pil Jae
PJ
D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response; D009997:Osmotic Pressure
D003470:Culture Media; D005656:Fungal Proteins; D002686:Chitin; D002687:Chitin Synthase